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From: zaluzec-at-microscopy.com
Date: Tue, 1 Jan 2002 08:59:58 -0600
Subject: Testing Jan 01, 2002

Contents Retrieved from Microscopy Listserver Archives
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This is just simple test to check that the new
files for 2002 are operating correctly.

Nestor


From daemon Tue Jan 1 13:15:26 2002



From: zaluzec-at-microscopy.com
Date: Tue, 1 Jan 2002 13:08:38 -0600
Subject: Administrivia: 2001 Archives and Search Engine Updated

Contents Retrieved from Microscopy Listserver Archives
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Colleagues...

Well, I had some spare time on my hands these last few days (a bad sign),
so I've finally got around to updating the Listserver Search Engine as so
many of you have requested.

You can now search the Microscopy Listserver Archives by
a specific Month/Year or if you choose for a complete Year at a time.
Just go to:

http://www.msa.microscopy.com/MicroscopyListserver/

and follow the Listserver Utilies links. Remember searching
a full year can take some time and produce a long output
page if your not careful with your search parameters.

A minor problem occurs when/if you do a "complete year " search
of the older archives. There will be a few anomolies in the output
files created as the file format for these very old data files changes alot,
and sometimes on a monthly basis. Fixing the formats is ALOT
of work, so if you supect problems just do a monthly search.

Hope you all enjoy the New Years present.


Nestor
Your Friendly Neighborhood SysOp


From daemon Tue Jan 1 17:36:08 2002



From: Earl Weltmer :      eweltmer-at-home.com
Date: Tue, 1 Jan 2002 15:28:23 -0800
Subject: Cold FE Guns

Contents Retrieved from Microscopy Listserver Archives
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Hi All & Happy New Year,

In my daydreaming & contemplating the universe I have come across a question
concerning FE guns that may seem like a stupid question to some but I would
like a response anyway.

Hitachi series cold FE guns have three elements: the FE tip, the extraction
or first anode (the equivalent to a wehnelt cap on conventional SEMs) & the
second anode ( the equivalent to the anode on standards SEMs).

Gun activation is done by a microprocessor in the following manner:

1. The FE tip & the first anode is raised to the primary voltage, say -15
KV.
2. The first anode voltage is reduced from the primary voltage in 100 volt
increments until the desired emission current is reached: usually 5 to 10
uA. The first anode voltage is nominally reduced from the primary voltage by
3 kv to 6.3 kv depending upon the filament life. In this example say it
takes 5 kv to extract the desired 10 uA of emission current, then the
filament is at -15 KV while the first anode is at -10 KV.
3. The second anode is at ground potential (0 volts).

I understand the physics behind this as electrons are extracted from the
source by the first anode. In this case the electrons are at a -15 KV
potential and continue to be accelerated down the column as the second anode
is at ground potential. All well & good.

The problem that I have is when the primary voltage is at say -1 kv.
Using the same filament and parameters, the filament is at -1 kv while the
first anode is at +4 kv.
The electrons that are extracted from the filament are at the primary
voltage: - 1 kv.

The first anode is now at +4 KV while the second anode is at 0 volts.
The electrons that are extracted are now between the first & second anode at
a -1 kv potential.
It would seem that when electrons are at the - 1 kv potential that they
would be attracted to the first anode as the first anode is now at +4 kv.
Instead the -1 kv electrons continue down the column in seeming defiance of
the laws of physics.

I have measured the voltages in question & I have examined the gun
components.

I am sure the laws of physics are intact but there is something that I am
missing that can be explained away (Dr. Fred Schamber are you listening?).

Not that this presents an immediate problem but just to satisfy my
curiosity.

Thanks to All.

Earl Weltmer
Scanservice Corp.




From daemon Wed Jan 2 09:02:16 2002



From: Simon Watkins :      swatkins+-at-pitt.edu
Date: Wed, 02 Jan 2002 09:52:59 -0500
Subject: Quantitative Fluorescence Microscopy 2002

Contents Retrieved from Microscopy Listserver Archives
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Folks:
I thought I should let you all know about the Fourth Annual Course in
Quantitative Fluorescence Microscopy to be taught between June 16 and
21st 2002 at the Mount Desert Island Marine Biology Laboratories in
Arcadia National Park in Maine.
This team taught course led by Dave Piston (Vanderbilt), Mike Nathanson
(Yale), Guillermo Romero (Pittsburgh) and myself focusses specifically
on the development and application of modern fluorescence microscopic
methods. This intensive course covers all aspects of the technology
from microscope and dye design, cameras, confocal microscopy, live cell
microscopy, multiphoton microscopy and GFP. Considerable attention is
also given to quantitative analysis in 2 and 3 dimensions and time. The
specific focus of the course allows an in depth treatment of these
methods.
The goal of the course it to teach students how to best implement these
methods within their labs, using either their own cells and tissues or
using material supplied by the course. An extensive array
instrumentation, provided by all the major microscope and associated
software, hardware and camera manufacturers will be available for
students to use. For the last 3 years it has been a very successful
event and we were encouraged to give the course again. A full
description of the course lectures together with lecture outlines,
registration etc. is available at http://sbic6.sbic.pitt.edu/microscopy.
I would encourage you to spread the word, or sign up if you are
interested.
The total number of students is limited to 20, it generally fills up
pretty early though final enrollment is decided by the course faculty.
If you have any further questions please feel free to contact me
Thanks
Simon

-------------------------------------------
Simon C. Watkins Ph.D. MRC Path
Professor Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
3500 Terrace St
University of Pittsburgh
Pittsburgh PA 15261
Tel: 412-648-3051
Fax: 412-383-8894
URL: http://www.cbi.pitt.edu
--------------------------------------------



From daemon Wed Jan 2 09:04:01 2002



From: =?iso-8859-1?q?Richard=20Muscat?= :      themuscat-at-yahoo.co.uk
Date: Wed, 2 Jan 2002 14:58:41 +0000 (GMT)
Subject: The Future?

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

My name is Richard Muscat and i am currently in
my
third year of studying biology at Lancaster
University, England. My final year dissertation is on
microscopes and a number of people recommended this
emailing list to me and encouraged to me to try and
obtain some information this way.

I understand that most of you on this emailing
list will be very busy but if you have any spare time
i
would really appreciate your views on where you think
microscopy is heading in the future.

Thank you very much for your time. Happy new year to
all.

Yours sincerly

Richard Muscat

Email: themuscat-at-yahoo.co.uk


Richard Muscat,
Lonsdale College,
Lancaster University,
Lancaster,
Lancs,
LA1 4YU
England


__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com


From daemon Wed Jan 2 12:45:43 2002



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Wed, 02 Jan 2002 13:30:58 -0500
Subject: Fwd: vibration isolation standards

Contents Retrieved from Microscopy Listserver Archives
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} Happy New Year to everyone!
}
} We are setting up a totally new EM/LM lab - being built from the ground
} up. We have told our engineers that we need to have a vibration-free
} environment for optimum equipment operation. They would like to know
} exactly what vibration is tolerable and what isn't. Are there any
} standards or measurements out there that detail what limits can be
} tolerated and what can't?
}
} Thank you!
}
} Lesley
}
} Lesley S. Bechtold
} Supervisor, Biological Imaging
} The Jackson Laboratory
} 600 Main St.
} Bar Harbor, ME 04609
} 207-288-6191

Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Wed Jan 2 12:56:15 2002



From: Julian Martinez Fernandez :      julianmartinezfernandez-at-hotmail.com
Date: Wed, 02 Jan 2002 19:50:20 +0100
Subject: Help about CDU EDAX detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleges:

We have recently purchased a “Phoenix” EDAX microanalysis system for our
Philips CM200 TEM microscope with a sapphire detector and CDU (Compact
Detecting Unit). We are currently waiting for the equipment to arrive.

The distributor of EDAX for Philips microscopes is suggesting now changing
the configuration and substituting the CDU unit for a 10-liter Liquid
Nitrogen Dewar, maintaining the original price. The reason that they give us
for this suggestion is that the nitrogen in the 10-liter detector last
longer than in the CDU unit. According to them, neither of these systems
needs to have nitrogen when they are not been used.

I would like advising in this matter because we always had thought that the
CDU unit had a superior performance.

Many thanks,
Julian

___________________
Julian Martinez Fernandez
University of Seville
Spain


_________________________________________________________________
MSN Photos es la manera más sencilla de compartir, editar e imprimir sus
fotos favoritas. http://photos.latam.msn.com/Support/WorldWide.aspx



From daemon Wed Jan 2 13:29:31 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Wed, 02 Jan 2002 13:22:33 -0600
Subject: Parts needed

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

We have an old (at least 1960's) Wild Heerbrugg Stereomicroscope that
needs a new fine focus. The model number is M7 S. The bit that
is broken is the rack and pinion with the focusing knob. This part
mounts to the microscope stand and the microscope head mounts into it.

Anyone out there know of someone who might have this part or a
substitute? The scope is a nice one and we can't afford to replace it
right now.

Thanks,
Karen Pawlowski, Ph.D.



From daemon Wed Jan 2 14:17:17 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Wed, 2 Jan 2002 14:07:10 -0600
Subject: Re: Cold FE Guns

Contents Retrieved from Microscopy Listserver Archives
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Your analysis is correct as far as it goes.

But you may have forgotten that the electrons passing through the
hole in Anode 1 each have 5 keV of kinetic energy. As they continue
on their merry past the +4 kV electrode, they slow down and this does
effect the aberration coefficients of the "electrostatic gun lens".

However, they reach Anode 2 before they slow down completely. In fact
they still have 1 keV of kinetic energy at this point and are now
able to zip down the column in a beam along the axis. The gun lens
will bring this beam to a focus at some point along the axis. In fact
the earliest CWICSCAN FE SEMs used only this lens to focus the beam.

Cheers,

Jim P.


}
}
} The problem that I have is when the primary voltage is at say -1 kv.
} Using the same filament and parameters, the filament is at -1 kv while the
} first anode is at +4 kv.
} The electrons that are extracted from the filament are at the primary
} voltage: - 1 kv.
}
} The first anode is now at +4 KV while the second anode is at 0 volts.
} The electrons that are extracted are now between the first & second anode at
} a -1 kv potential.
} It would seem that when electrons are at the - 1 kv potential that they
} would be attracted to the first anode as the first anode is now at +4 kv.
} Instead the -1 kv electrons continue down the column in seeming defiance of
} the laws of physics.
}
} I have measured the voltages in question & I have examined the gun
} components.
}
} I am sure the laws of physics are intact but there is something that I am
} missing that can be explained away (Dr. Fred Schamber are you listening?).
}
} Not that this presents an immediate problem but just to satisfy my
} curiosity.
}
} Thanks to All.
}
} Earl Weltmer
} Scanservice Corp.

--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Wed Jan 2 16:48:58 2002



From: Victor Sidorenko :      antron-at-space.ru
Date: Thu, 3 Jan 2002 01:30:24 +0300
Subject: Re: Cold FE Guns

Contents Retrieved from Microscopy Listserver Archives
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Dear Earl!

I think, not all depends on voltages on anodes and tip only.
It is necessary to consider as well where equipotential
curves are located, i.e. what is the field strength picture,
i.e. it is necessary to analyze also the form of electrodes.
The similar situation exists in a mirror electron
microscope: electrons are braked near sample, but reach it,
because previously were accelerated.
Regards and season greetings,

Victor Sidorenko, ANTRON Ltd., Moscow, Russia.

EW} Hi All & Happy New Year,

EW} In my daydreaming & contemplating the universe I have come across a question
EW} concerning FE guns that may seem like a stupid question to some but I would
EW} like a response anyway.

EW} Hitachi series cold FE guns have three elements: the FE tip, the extraction
EW} or first anode (the equivalent to a wehnelt cap on conventional SEMs) & the
EW} second anode ( the equivalent to the anode on standards SEMs).

EW} Gun activation is done by a microprocessor in the following manner:

EW} 1. The FE tip & the first anode is raised to the primary voltage, say -15
EW} KV.
EW} 2. The first anode voltage is reduced from the primary voltage in 100 volt
EW} increments until the desired emission current is reached: usually 5 to 10
EW} uA. The first anode voltage is nominally reduced from the primary voltage by
EW} 3 kv to 6.3 kv depending upon the filament life. In this example say it
EW} takes 5 kv to extract the desired 10 uA of emission current, then the
EW} filament is at -15 KV while the first anode is at -10 KV.
EW} 3. The second anode is at ground potential (0 volts).

EW} I understand the physics behind this as electrons are extracted from the
EW} source by the first anode. In this case the electrons are at a -15 KV
EW} potential and continue to be accelerated down the column as the second anode
EW} is at ground potential. All well & good.

EW} The problem that I have is when the primary voltage is at say -1 kv.
EW} Using the same filament and parameters, the filament is at -1 kv while the
EW} first anode is at +4 kv.
EW} The electrons that are extracted from the filament are at the primary
EW} voltage: - 1 kv.

EW} The first anode is now at +4 KV while the second anode is at 0 volts.
EW} The electrons that are extracted are now between the first & second anode at
EW} a -1 kv potential.
EW} It would seem that when electrons are at the - 1 kv potential that they
EW} would be attracted to the first anode as the first anode is now at +4 kv.
EW} Instead the -1 kv electrons continue down the column in seeming defiance of
EW} the laws of physics.

EW} I have measured the voltages in question & I have examined the gun
EW} components.

EW} I am sure the laws of physics are intact but there is something that I am
EW} missing that can be explained away (Dr. Fred Schamber are you listening?).

EW} Not that this presents an immediate problem but just to satisfy my
EW} curiosity.

EW} Thanks to All.

EW} Earl Weltmer
EW} Scanservice Corp.



From daemon Wed Jan 2 17:44:58 2002



From: David Stokes :      stokes-at-saturn.med.nyu.edu
Date: Wed, 02 Jan 2002 18:33:03 -0500
Subject: TEM: NYSBC faculty positions in cryoEM

Contents Retrieved from Microscopy Listserver Archives
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POSITIONS IN CRYOELECTRON MICROSCOPY

NEW YORK STRUCTURAL BIOLOGY CENTER

The New York Structural Biology Center (http://www.nysbc.org ) seeks
both junior and senior applicants for two faculty level positions in the
field of Cryoelectron Microscopy.The Center was founded by nine New York
academic research institutions* to implement high-end, state-of-the art
instrumentation for both NMR and Cryoelectron Microscopy in order to
stimulate their institutional research programs. The Center is
purchasing three cryoelectron microscopes at 120, 200, and 300 kV, the
last with a liquid-helium stage and an energy filter.In addition to
housing independent investigators with active research programs, the
Center will serve as a research resource for consortium members,
providing local investigators with excellent opportunities for
collaboration on a wide array of biological targets.We now seek
applicants for two CryoEM positions at the Center who have a record of
excellent research achievement and active research programs in any of
three CryoEM fields: tomography, single-particles, or
crystallography.Send a biographical sketch, a brief statement of
research accomplishments and future plans, together with names and
addresses of three individuals who can provide letters of
recommendation.Applications should be sent as soon as possible to:
CryoEM Search Committee, at nysbc-at-nysbc.org .

* Albert Einstein College of Medicine, Columbia University, City
University of New York, Memorial Sloan-Kettering Cancer Center, Mt.
Sinai School of Medicine, New York University, Rockefeller University,
Wadsworth Center, Weill Medical College at Cornell University.
** this ad will appear in Jan 3 issue of Nature and can be accessed at
http://www.nysbc.org/cem1.htm



--
------------------------------
David L. Stokes
Skirball Institute
NYU Medical Center
540 First Ave
New York, NY 10016
tel and FAX: 212-263-1580
http://saturn.med.nyu.edu/~stokes
------------------------------





From daemon Wed Jan 2 17:45:36 2002



From: David Stokes :      stokes-at-saturn.med.nyu.edu
Date: Wed, 02 Jan 2002 18:34:27 -0500
Subject: TEM: EM manager position at NYSBC

Contents Retrieved from Microscopy Listserver Archives
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MANAGER IN CRYOELECTRON MICROSCOPY
NEW YORK STRUCTURAL BIOLOGY CENTER

The New York Structural Biology Center ( http//www.nysbc.org )
seeks a technical manager for a high-end, state of the art facility in
Cryoelectron Microscopy. The facility will include three cryoelectron
microscopes at 120, 200, and 300 kV, the last with a liquid-helium stage

and an energy filter, as well as all necessary ancillary equipment. The

facility will be used by investigators from nine New York academic
research institutions*, and for in-Center researchers, on a broad range
of biological targets employing any of three CryoEM methodologies:
tomography, single-particles, and crystallography. The appointee will
act initially as liaison between scientists and manufacturers in
developing specifications, testing, and installing the three
instruments, and later in maintaining their performance, and in
supporting user applications and new developments. In addition, the
appointee will implement a variety of specialized technologies
associated with cryoEM, especially for automated data collection. A
strong technical background in electron microscopy is essential and
familiarity with biological samples would be a bonus. Send a
biographical sketch, a brief statement of previous research experience,
together with names and addresses of three individuals who can provide
letters of recommendation. Applications should be sent as soon as
possible to: CryoEM Search Committee, at nysbc-at-nysbc.org .

* Albert Einstein College of Medicine, Columbia University, City
University of New York, Memorial Sloan-Kettering Cancer Center, Mt.
Sinai School of Medicine, New York University, Rockefeller University,
Wadsworth Center, Weill Medical College at Cornell University.
** this ad can be accessed at http://www.nysbc.org/cemm2.htm



--
------------------------------
David L. Stokes
Skirball Institute
NYU Medical Center
540 First Ave
New York, NY 10016
tel and FAX: 212-263-1580
http://saturn.med.nyu.edu/~stokes
------------------------------





From daemon Wed Jan 2 18:01:33 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 02 Jan 2002 15:55:56 -0800
Subject: Re: Help about CDU EDAX detectors

Contents Retrieved from Microscopy Listserver Archives
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I've been looking at the CDU as part of their "Falcon" system.

From what I have seen, the CDU has the same performance
as the 10L detector. Supposedly, the 10L detector can be
fitted with a smaller dewar. The idea behind the CDU is that
it is intended for intermittent use. i.e., not used everyday.
So its Dewar capacity is smaller but same performance as
big Dewar unit. LN2 is poured in (smaller amount) and
in an hour, the system is ready to go. The LN2 in the CDU
might last only a day or so, whereas the larger Dewar
would keep the detector cold for more days.

As far as I can see, it is an issue of frequency of use
and how often, therefore, you have to fill the Dewar.

gary g.


At 10:50 AM 1/2/2002, you wrote:

} Dear colleges:
}
} We have recently purchased a “Phoenix” EDAX microanalysis system
} for our Philips CM200 TEM microscope with a sapphire detector and CDU
} (Compact Detecting Unit). We are currently waiting for the equipment to arrive.
}
} The distributor of EDAX for Philips microscopes is suggesting now
} changing the configuration and substituting the CDU unit for a 10-liter
} Liquid Nitrogen Dewar, maintaining the original price. The reason that
} they give us for this suggestion is that the nitrogen in the 10-liter
} detector last longer than in the CDU unit. According to them, neither of
} these systems needs to have nitrogen when they are not been used.
}
} I would like advising in this matter because we always had
} thought that the CDU unit had a superior performance.
}
} Many thanks,
} Julian
}
} ___________________
} Julian Martinez Fernandez
} University of Seville
} Spain
}
}
} _________________________________________________________________
} MSN Photos es la manera más sencilla de compartir, editar e imprimir sus
} fotos favoritas. http://photos.latam.msn.com/Support/WorldWide.aspx
}



From daemon Wed Jan 2 19:32:29 2002



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Wed, 02 Jan 2002 21:02:13 -0500
Subject: Re: Help about CDU EDAX detectors

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Pawley,

This makes sense.
I assumed that the electrons had only -1 kv of energy as the filament is a -
1kv.

Thank You for you explanation.

Now perhaps I can daydream about other more important issues.

Happy New Year.

Earl Weltmer



----- Original Message -----
} From: "James Pawley" {jbpawley-at-facstaff.wisc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 02, 2002 12:07 PM


We have had a CDU/Sapphire detector on an SEM for over 2 years, and it does
work as EDAX promised. It does save Liq. N2 if you have an environment in
which the EDX analysis is performed only infrequently. I have discerned no
degradation in performance in that time, and obviously icing is not a
problem. It is much lighter than the 10L system, and that might alter the
mechanical effects on the microscope column, but the 10L system is fine in
that regard anyway.

There are downsides, though. It will not stay cold over the weekend, and
takes a couple of hours to cool after you fill it. This means that you
can't do EDX on a Monday morning, and you (or your users, in a multi-user
facility) have to remember to check that the dewar is cold a couple of
hours before starting work, but without interfering with other users of the
microscope. On a high-resolution TEM this might (would?) also degrade the
thermal stability. If your EDX workload is heavy, the CDU will take more
of your technician's time, as it will have to be filled more often to keep
it cold.

I'm not aware of any way in which the CDU is supposed to have any technical
advantage over the 10L system.

Each potential customer will have to judge the relative merits. The
hardware works, at least for us, as advertised.

Tony Garratt-Reed

At 07:50 PM 1/2/2002 +0100, Julian Martinez Fernandez wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jan 2 21:13:40 2002



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Wed, 02 Jan 2002 22:06:57 -0500
Subject: Re: Fwd: vibration isolation standards

Contents Retrieved from Microscopy Listserver Archives
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There are some of us who have spent most of the last 20 years trying to
justify how to move our labs to the coast of Maine, just to get the granite
bedrock!

Vibration, sound and electromagnetic interference are all huge unknowns,
when it comes to microscope performance. Each manufacturer will provide
you with a set of specifications which are "required" for their instrument
to meet its specifications. I would strongly suggest that you build a new
lab to be significantly better than those specs, because the lab will
(hopefully!) outlast the instruments you buy now. New instruments will, in
all probability, have tighter specs than the present ones. Not only that,
but not even the manufacturer KNOWS, for certain, that the instrument will
work, even in an environment that meets the specs.. Only eating the
pudding will provide the answer!

This, by the way, is because the specifications are entirely empirical.
There is no magic formula a manufacturer can use, plugging in various
pieces of information about the microscope, which tells them the tolerable
interference levels. They just pluck a figure from the air (well, based on
the experience of *lots* of earlier installations, and their own lab
instruments), and hope for the best. If your installation has
difficulties, the next customer will find the specs tightened. Each site
is slightly different, and can present some new twist of vibrational
frequency, direction, or whatever, that can excite a previously unknown
resonance in the microscope system. Alternatively, perhaps, your system
may be subtly different from others (a new batch of wire for some of the
springs in the stage, for example, changing the resonant frequencies), so
it respondes differently.

Some listers may not agree with my next comment, but in my experience,
manufacturers will not abandon you if your site is a few percent out of
spec - they will work with you, within reason, to get the instrument
running well (it is bad publicity for them otherwise). The difference is
that you may have to pay for extra amelioration, whereas if your site is in
spec, they will (usually) pay.

Intuitively, granite would be thought to be a good site - after all, don't
we try to put pilings down to bedrock to get stable sites in other areas.
However, it also transmits vibrations very well if any are induced.
Sandstone, I am told, is much better, because it damps the vibrations much
more. Any of them, I am certain, are better than the mud-filled
saltmarshes on which MIT is built (hence my opening remark!).

So where does that leave us, as users? Architects will ask you for the
"Specifications" of the instruments you want to install, and will find the
cheapest way of meeting them. In 1980, our EM site easily met the
requirements for our EM300 and JEOL200CX. Surprise, surprise - it doesn't
come close for a modern FEG-IVEM! On the other hand, given your location,
is there much that can be done? Usually one tries to reach the bedrock,
but in your case, the bedrock reaches you. It may be a case of what you
have is all you can get. If there is too much vibration on your floor, you
may have to live with what improvement an isolation system can provide
(modern active ones are very effective).

Get qualified, experienced engineers to perform your surveys. Your
architects should have contact with people they have worked with in the
past, and the microscope vendors certainly have such contacts. Don't
forget acoustical interference, electromagnetic problems (magnetic fields,
once generated, cannot be eliminated - only moved!). Ask for - nay, DEMAND
- the best site money can buy. It will, in the long run, be a good
investment for you lab.

By the way, very little of the above represents quality scientific research
- just a lot of strongly held subjective opinions formed over the years.
Good lock!

Tony Garratt-Reed

At 01:30 PM 1/2/2002 -0500, Lesley S. Bechtold wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jan 3 02:23:21 2002



From: rajdeep-at-aripune.ernet.in (Rajdeep Dongre)
Date: Thu, 03 Jan 02 11:12:07 PST
Subject: SEM Problem

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Friends,

I have two problems regarding SEM Stereoscan S120:

1. Do you no any supplier who can supply with Spray Aperture? Tell me
address of Indian suppliers, if you know any?

2. Tell why I am getting poor quality image and a very astigmatic
image, even at high KV. I have cleaned the column many times but
cannot get improvement in the quality of the image.

a) Is it because of the problem of Spray Aperture? [I am use since
12 years]

OR

b) I found the screw attached with the electro-optic column magnetic
in nature. I have changed it. Is it because of the column having
similar problem?

Thanks in advance.

Rajdeep Dongre
Electron Microscopy Laboratory
Agharkar Research Institute
G.G. Agarkar Road
Pune - 411 004, India



From daemon Thu Jan 3 05:31:08 2002



From: =?ISO-8859-1?Q?=E4=BE=BA=D9=C5=C2=EC_=B9=C7=C5=B9=D4=C5_=28PAIBOON_?=
Date: Thu, 3 Jan 2002 18:23:38 +0700 (ICT)
Subject: Re: SEM Problem

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Dear Dongre


Most problems of poor image quality and astigmatism are from
objective aperture and for the image contrast is from scintillator
(electron
detector) .
Clean column it does mean clean or new apertures as well.
No effect from the scews.

Good luck

Paiboon Nuannin
Scientific Equipment Center
Prince of Songkla University
Hatyai
Thailand


On Thu, 3 Jan 2002, Rajdeep Dongre wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Friends,
}
} I have two problems regarding SEM Stereoscan S120:
}
} 1. Do you no any supplier who can supply with Spray Aperture? Tell me
} address of Indian suppliers, if you know any?
}
} 2. Tell why I am getting poor quality image and a very astigmatic
} image, even at high KV. I have cleaned the column many times but
} cannot get improvement in the quality of the image.
}
} a) Is it because of the problem of Spray Aperture? [I am use since
} 12 years]
}
} OR
}
} b) I found the screw attached with the electro-optic column magnetic
} in nature. I have changed it. Is it because of the column having
} similar problem?
}
} Thanks in advance.
}
} Rajdeep Dongre
} Electron Microscopy Laboratory
} Agharkar Research Institute
} G.G. Agarkar Road
} Pune - 411 004, India
}
}



From daemon Thu Jan 3 11:25:25 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 03 Jan 2002 11:17:00 -0600
Subject: Thanks for Parts lead

Contents Retrieved from Microscopy Listserver Archives
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Thankyou everyone who responded for my plea for a lead on who might be
able to fix or get parts for our old Wild Heerbruug microscope.
You people are great!

Karen Pawlowski



From daemon Thu Jan 3 11:33:24 2002



From: Mick Thomas :      mgt3-at-ccmr.cornell.edu
Date: Thu, 03 Jan 2002 12:29:06 -0500
Subject: basic bio TEM questions

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Fellow microscopists,

I was recently asked for some help with TEM of biological specimens
(cells). Since I work in the materials side of microscopy, I could answer
some questions but not all. I would appreciate any help with the following
questions:

1) Specimen thickness: How thick can a cell structure be and still be able
to resolve 20nm features at 100keV? 300keV?
2) Beam damage: What sort of damage typically occurs to such specimens and
are cold stages required?
3) Charging: Do bio specimens need to be coated for TEM (to dissipate the
charge) and if so what kind of coating is used and how thick?
4) Contrast: Are such specimens typically stained and if so what sort of
staining is used?

I realize many volumes could probably be written on each of the above, but
any help pointing us in the right direction would be very much appreciated.

Thanks,

Mick Thomas

-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu



From daemon Thu Jan 3 12:27:01 2002



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Thu, 03 Jan 2002 10:22:23 -0800
Subject: Re: Fwd: vibration isolation standards

Contents Retrieved from Microscopy Listserver Archives
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Lesley,
There is, in Physics Today an article about the Triebenberg Lab that Hannes Lichte has built to isolate his high-resolution microscopes from vibration, fields, etc. He told me last January that the information limit of his microscope went from 1.2A to 0.9A just by relocating it to the new
facility. The article is at http://physicstoday.org/pt/vol-54/iss-3/p24.html
Also, check out:
“Design and implementation of a site for a one-Ångstrom TEM”, John H. Turner, Michael A. O’Keefe and Robert Mueller, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1177-1178.
“The Triebenberg laboratory -- Designed for highest resolution electron microscopy and holography”, Hannes Lichte et al., in 59th Ann. Proc. MSA, Long Beach, California (2001) 894-895, Microscopy & Microanalysis 7, suppl.2.
Happy New Year,
Michael A. O'Keefe

"Lesley S. Bechtold" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } Happy New Year to everyone!
} }
} } We are setting up a totally new EM/LM lab - being built from the ground
} } up. We have told our engineers that we need to have a vibration-free
} } environment for optimum equipment operation. They would like to know
} } exactly what vibration is tolerable and what isn't. Are there any
} } standards or measurements out there that detail what limits can be
} } tolerated and what can't?
} }
} } Thank you!
} }
} } Lesley
} }
} } Lesley S. Bechtold
} } Supervisor, Biological Imaging
} } The Jackson Laboratory
} } 600 Main St.
} } Bar Harbor, ME 04609
} } 207-288-6191
}
} Lesley S. Bechtold
} Supervisor, Biological Imaging
} The Jackson Laboratory
} 600 Main St.
} Bar Harbor, ME 04609
} 207-288-6191



From daemon Thu Jan 3 15:04:21 2002



From: Nguyen Hoan :      hoan-at-opea.com
Date: Thu, 03 Jan 2002 21:50:52 +0100
Subject: Electron Energy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
The electrons are emitted from the cathode. They go down to the earth
potential. Their energy is the potential between cathode and earth, even

when there are any kind or number of potential on their trajectory.
Happy New Year
Hoan

--

Hoan Nguyen
OPEA
114 rue de la Jarry
94300 VINCENNES (F)
Tél. 33 1 43283496
Fax. 33 1 43280364
E-mail: hoan-at-opea.com
WEB: www.opea.com





From daemon Thu Jan 3 15:12:43 2002



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Thu, 03 Jan 2002 16:12:54 -0500
Subject: service contract for microscope with Specialty Underwriters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I would appreciate very much if anyone could provide me information on the
pit falls to pursue microscope service contract with third party (in
particular, the Specialty Underwriters) instead of Jeol. Inc.

We have one Jeol SEM and one Jeol-2010 TEM to be put on contract this
year. I have encountered difficulties in persuading our purchasing agent
to go with Jeol Inc., because Specialty Underwriters has a quotation $1,000
lower. Because of the State Law, we need to provide evidence not to choose
the lowest price vendor. I read comments concerning the pit falls of the
service contract with third party on the server. But I would really need
hard data.

It will be great if anyone could help.

Best Regards
Yan Xin


=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Fri Jan 4 00:48:40 2002



From: M, Prabhakar (CORP, GEITC) :      M.Prabhakar-at-geind.ge.com
Date: Fri, 4 Jan 2002 12:08:50 +0530
Subject: SEM Problem

Contents Retrieved from Microscopy Listserver Archives
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Dongre,
Refer to problem no 2.
Poor image quality/Astigmatism could be due to various reasons.
If the scintillator tip is coloured or damaged, you might try replacing it.
Check to see if the pole pieces are not misaligned. Make sure that the gap
is at the lower end of the pole piece assembly. (Align the gun with spot
size 5-6)
If you fail after above rectifications, you should try removing the
condensor lenses and clean the stigmator assembly. Minutest dust sitting in
this area can result in poor image/image movement/astigmatism.
Regards
Prabhakar





-----Original Message-----
} From: rajdeep-at-aripune.ernet.in [mailto:rajdeep-at-aripune.ernet.in]
Sent: Friday, January 04, 2002 12:42 AM
To: microscopy-at-sparc5.microscopy.com


Dear Friends,

I have two problems regarding SEM Stereoscan S120:

1. Do you no any supplier who can supply with Spray Aperture? Tell me
address of Indian suppliers, if you know any?

2. Tell why I am getting poor quality image and a very astigmatic
image, even at high KV. I have cleaned the column many times but
cannot get improvement in the quality of the image.

a) Is it because of the problem of Spray Aperture? [I am use since
12 years]

OR

b) I found the screw attached with the electro-optic column magnetic
in nature. I have changed it. Is it because of the column having
similar problem?

Thanks in advance.

Rajdeep Dongre
Electron Microscopy Laboratory
Agharkar Research Institute
G.G. Agarkar Road
Pune - 411 004, India



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From daemon Fri Jan 4 01:33:21 2002



From: PECZ Bela :      pecz-at-mfa.kfki.hu
Date: Fri, 4 Jan 2002 08:35:58 +0100
Subject: Re: Fwd: vibration isolation standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleague,

I took the information leaflet of JEOL 3010 and found for floor
vibration: 1 micrometer at 2 Hz and 2 micrometer from 3 to 9 Hz. External
magnetic field should be 0.1 microTesla or less. (Of course I knew this data
by heart, as our microscope was installed last November.)
However, it is surprising, that Philips advertising materials do not
contain the specs for the installation room.
For the antivibration table we went down to 3 m and made a block of
concrete, which is isolated from the sorrounding by several other layers,
the most important is a 5 cm layer of cork. Strain magnetic field was
decreased by changing the second ceiling of the room for a wooden one.
Anyway, the microscope works well, 0.122 nm is very nice on images (Al,
311), even a colleague took 440 spacings of GaAs, which is just below 1
Angstrom, while the guarranted line resolution of the microscope is 1.40
Angstrom.
We take nice images, despite we do not have any CCD camera on the
microscope. By the way, does someone know a cheap solution for that?
Sorry, back to the original topic, once the new EM lab will be a
biological one, I think no high resolution is needed, the simple
antivibration table we ordered should be anough for you. Tell me if you need
images on how it was built at the different stages of the work.
Good luck, Bela Pecz
-------------------------------------------------------
Dr. Béla Pécz
Head of the Thin Films Physics Laboratory
Research Institute for Technical Physics and Matl. Sci.
H-1525 Budapest, POBox 49
Hungary
phone: 36-1-392-2587
fax: 36-1-275-4996
E-Mail: pecz-at-mfa.kfki.hu
http://www.mfa.kfki.hu/int/thin/
http://www.mfa.kfki.hu/~pecz/
-------------------------------------------------------





} "Lesley S. Bechtold" wrote:
} } } Happy New Year to everyone!
} } }
} } } We are setting up a totally new EM/LM lab - being built from the ground
} } } up. We have told our engineers that we need to have a vibration-free
} } } environment for optimum equipment operation. They would like to know
} } } exactly what vibration is tolerable and what isn't. Are there any
} } } standards or measurements out there that detail what limits can be
} } } tolerated and what can't?
} } }
} } } Thank you!
} } }
} } } Lesley
} } }
} } } Lesley S. Bechtold
} } } Supervisor, Biological Imaging
} } } The Jackson Laboratory
} } } 600 Main St.
} } } Bar Harbor, ME 04609
} } } 207-288-6191
} }
} } Lesley S. Bechtold
} } Supervisor, Biological Imaging
} } The Jackson Laboratory
} } 600 Main St.
} } Bar Harbor, ME 04609
} } 207-288-6191
}
}



From daemon Fri Jan 4 02:29:53 2002



From: Janos Labar :      labar-at-mfa.kfki.hu
Date: Fri, 4 Jan 2002 09:40:22 +0100
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe



From daemon Fri Jan 4 09:35:20 2002



From: JHoffpa464-at-aol.com
Date: Fri, 04 Jan 2002 10:26:47 EST
Subject: cincinnati EM people

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hello all, any univ of cinci EM techs or EM lab managers here. i will be traveling to cinci on buisness at the univ of cinci. would be nice to know someone there, perhaps to talk with if i run into problems. email me back.
John Hoffpauir
Thomas Jefferson University
Philadelphia Pa


From daemon Fri Jan 4 10:22:36 2002



From: James Martin :      james.s.martin-at-att.net
Date: Fri, 4 Jan 2002 11:17:08 -0500
Subject: contract lab for thin-sections by cryotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings in this new year.

I need cryo sections made of multi-layer polymer films (PE/EVA). Any
recommendations for a contract lab that does cryo sectioning?

James Martin



From daemon Fri Jan 4 11:12:52 2002



From: Petr Schauer :      Petr-at-isibrno.cz
Date: Fri, 4 Jan 2002 18:06:35 +0100
Subject: Re: List of Microscopy Meetings for 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

My apologies for troubles with my Petr's Microscopy Resources
(http://www.petr.isibrno.cz/microscopy/) during the Christmas and/or
New Year Holiday. The server possessed hardware problems and no
repair was possible, because I was out of institute. Now, the server
is after complete reinstallation and all problems are fixed.

In spite of this, all the links (suggested for the inclusion to the
list of meetings of 2002) have been added.

Regards,

Petr

} Dear Microscopists,
}
} I should like to inform you, that the list of microscopy meetings for
} the year 2002 at so called Petr's Microscopy Resources has been
} extended. You can see it at the
}
} http://www.petr.isibrno.cz/microscopy/meetings.php#2002
}
} Regards,
}
} Petr Schauer
} +---------------------------------------------------------------------+
} | Dr. Petr Schauer tel.: (+420 5) 41514313 |
} | Head of Electron Microscopy Laboratory fax : (+420 5) 41514404 |
} | INSTITUTE OF SCIENTIFIC INSTRUMENTS (+420 5) 41514402 |
} | ACADEMY OF SCIENCES OF THE CZECH REPUBLIC e-mail: petr-at-isibrno.cz |
} | Kralovopolska 147, CZ-612 64 Brno petr-at-dp.fce.vutbr.cz |
} | Czech Republic www: http://www.petr.isibrno.cz/ |
} +---------------------------------------------------------------------+
}
}




From daemon Fri Jan 4 12:45:42 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 4 Jan 2002 13:33:48 -0500
Subject: RE: vibration isolation standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try: http://www.vibeng.com/microscopy.htm

Happy New Year,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


} ----------
} From: Lesley S. Bechtold
} Sent: Wednesday, January 2, 2002 1:30 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Fwd: vibration isolation standards
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} } Happy New Year to everyone!
} }
} } We are setting up a totally new EM/LM lab - being built from the ground
} } up. We have told our engineers that we need to have a vibration-free
} } environment for optimum equipment operation. They would like to know
} } exactly what vibration is tolerable and what isn't. Are there any
} } standards or measurements out there that detail what limits can be
} } tolerated and what can't?
} }
} } Thank you!
} }
} } Lesley
} }
} } Lesley S. Bechtold
} } Supervisor, Biological Imaging
} } The Jackson Laboratory
} } 600 Main St.
} } Bar Harbor, ME 04609
} } 207-288-6191
}
} Lesley S. Bechtold
} Supervisor, Biological Imaging
} The Jackson Laboratory
} 600 Main St.
} Bar Harbor, ME 04609
} 207-288-6191
}
}
}


From daemon Fri Jan 4 13:51:11 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 04 Jan 2002 14:45:16 -0500
Subject: Cryo-Sectioning PE/EVA for TEM

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

James Martin wrote:
=====================================================================
I need cryo sections made of multi-layer polymer films (PE/EVA). Any
recommendations for a contract lab that does cryo sectioning?
=====================================================================
You can consider the laboratories of Structure Probe, Inc. an independent
analytical laboratory. We have been doing this kind of work for clients
since 1970. We are experienced with multi-layer polymer films generally,
and the PE/EVA system specifically. We are fully equipped to do this kind
of work in-house.

We are accredited by the American Association for Laboratory Accreditation
to the standard is ISO Guide 17025. More information about our laboratory
services capabilities can be found on our website URL
http://www.2spi.com/ils/ils.html

Let me know how we can help you.

Chuck

Disclaimer: Structure Probe, Inc. and SPI Supplies operate as one corporate
entity and provide both analytical services as well as products for
microscopy and microanalysis.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Fri Jan 4 15:22:28 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 04 Jan 2002 13:11:19 -0800
Subject: Re: SEM Problem

Contents Retrieved from Microscopy Listserver Archives
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I'm not familiar with this system but I suspect that
the basic principles are very close to those of other
SEMs.

I view astigmatism and resolution as two separate topics.
Mostly because they seem to be caused and cured by
mostly different areas in the SEM column. Bad stigmatism
would be seen when the two stig pots are rotated beyond
the 2 O'clock and 10 O'clock positions--basically, +- 45
degrees from zero. If your system has a final aperture
holder with more than one final aperture, move to another
aperture and re-stig. If the stig pots' position moves significantly
between apertures, then most likely, you have dirty final
apertures. If, in the other hand, the other apertures make
little difference in the position of the stig pots, then I would
guess that the scan coil liner is dirty. This little bugger can
have a huge effect on obtaining high resolution images.

The column liner seems to not have much effect on overall
performance--unless it is really dirty. It may also have
an aperture and that should be replaced on a routine
basis. The anode cap may also have one or two apertures
and these too should be replaced. A good check for a
dirty column liner is to obtain an image, increase magnification
to around 10KX. Then, rotate one of the beam alignment
knobs (pots for electronic position, not mechanical alignment
knobs on column) until it stops. Your image will of course
go away. Let it sit for about 30 seconds and then rotate
the pot back into position to re-establish the image.
Now watch the image and see if it drifts up or down or
left to right. If it does, odds are that your column liner
is dirty. If it does not, the liner is clean.

You should be able to find apertures from most any
of the large SEM materials suppliers, like Pella, SPI,
Ladd, etc. The quality seems to vary for regular
Pt apertures. Try different suppliers products until
you find one that works best for you. Any 12 year old
aperture I would think is long past its useful lifetime.

Would need more info about what you are talking about
relative to the magnetic screw. What brought the screw
into issue in the first place?

gary g.


At 11:12 AM 1/3/2002, you wrote:

} Dear Friends,
}
} I have two problems regarding SEM Stereoscan S120:
}
} 1. Do you no any supplier who can supply with Spray Aperture? Tell me
} address of Indian suppliers, if you know any?
}
} 2. Tell why I am getting poor quality image and a very astigmatic
} image, even at high KV. I have cleaned the column many times but
} cannot get improvement in the quality of the image.
}
} a) Is it because of the problem of Spray Aperture? [I am use since
} 12 years]
}
} OR
}
} b) I found the screw attached with the electro-optic column magnetic
} in nature. I have changed it. Is it because of the column having
} similar problem?
}
} Thanks in advance.
}
} Rajdeep Dongre
} Electron Microscopy Laboratory
} Agharkar Research Institute
} G.G. Agarkar Road
} Pune - 411 004, India



From daemon Fri Jan 4 21:27:53 2002



From: nicholas.welham-at-anu.edu.au ()
Date: Fri, 4 Jan 2002 21:18:22 -0600
Subject: Ask-A-Microscopist: heating stage for an inverted metallographic

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (nicholas.welham-at-anu.edu.au) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on
Thursday, January 3, 2002 at 15:44:19
---------------------------------------------------------------------------

Email: nicholas.welham-at-anu.edu.au
Name: Nick Welham

Organization: Australian National University

Education: Graduate College

Location: Canberra, Australia

Question: Probably not the best place to ask this, but here goes....
I have just "inherited" from a store room a Unitron HHS vacuum
heating stage for an inverted metallographic microscope.
Unfortunately, there are no instructions with it and I'm reluctant to
try using it until I have tried getting some instructions. Unitron no
longer have a copy, can you think of anywhere else which may have a
copy I could buy/borrow/get a copy of?
regards
Nick

---------------------------------------------------------------------------


From daemon Fri Jan 4 21:27:53 2002



From: dwhite-at-HuntingtonIndiana.Com ()
Date: Fri, 4 Jan 2002 21:17:52 -0600
Subject: Ask-A-Microscopist: microscopes to do comparisons

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dwhite-at-HuntingtonIndiana.Com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
January 3, 2002 at 13:42:49
---------------------------------------------------------------------------

Email: dwhite-at-HuntingtonIndiana.Com
Name: Dawn White

Organization: Marion General Hospital

Education: Graduate College

Location: Huntington, Indiana

Question: I see people using expensive comparison microscopes to do
comparisons (hairs, fibres, bullets etc)I was very surprised when I
heard that these things cost $50,000 or more !

Would it not be cheaper to use a sterio microscope and camera like we
have in our lab and compare the photographs ? This system cost less
than $2,000 and would seem like a cost effective option.

---------------------------------------------------------------------------


From daemon Fri Jan 4 22:25:14 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 04 Jan 2002 23:22:46 -0500
Subject: Re: Fwd: vibration isolation standards

Contents Retrieved from Microscopy Listserver Archives
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Lesley,
All of your equipment manufacturers should have specs for vibration. A
long long long time ago I worked for ETEC and their vibration spec was
not more than 10 micro inches displacement in any axis. In general,
frequencies below 17 Hz presented the largest problems. Each instrument
is different.

Ken Converse
owner (wish I were in Maine)
Quaity Images
Delta, PA

Lesley S. Bechtold wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} } Happy New Year to everyone!
} }
} } We are setting up a totally new EM/LM lab - being built from the
} } ground up. We have told our engineers that we need to have a
} } vibration-free environment for optimum equipment operation. They
} } would like to know exactly what vibration is tolerable and what
} } isn't. Are there any standards or measurements out there that detail
} } what limits can be tolerated and what can't?
} }
} } Thank you!
} }
} } Lesley
} }
} } Lesley S. Bechtold
} } Supervisor, Biological Imaging
} } The Jackson Laboratory
} } 600 Main St.
} } Bar Harbor, ME 04609
} } 207-288-6191
}
}
} Lesley S. Bechtold
} Supervisor, Biological Imaging
} The Jackson Laboratory
} 600 Main St.
} Bar Harbor, ME 04609
} 207-288-6191
}
}
}
}



From daemon Fri Jan 4 22:37:43 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 04 Jan 2002 23:37:07 -0500
Subject: Re: Fwd: vibration isolation standards

Contents Retrieved from Microscopy Listserver Archives
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Tony,
I heard about a microscope a long time ago that was sited in a
sub-basement on bedrock. Everyone was very happy.............until it
was installed. Vibes out the wazoo! Turns out US Steel had a
drop-forge plant on the same piece of bedrock about a mile away.

Some say an isolated cement slab on sand gives terrific isolation. I
know sand does a great job isolating 100 year old cast iron water pipe
from nearby dynamite. (Don't ask) Apparently your Charles River mud
transmits too well, huh?

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Tony Garratt-Reed wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} There are some of us who have spent most of the last 20 years trying to
} justify how to move our labs to the coast of Maine, just to get the granite
} bedrock!
}
} Vibration, sound and electromagnetic interference are all huge unknowns,
} when it comes to microscope performance. Each manufacturer will provide
} you with a set of specifications which are "required" for their instrument
} to meet its specifications. I would strongly suggest that you build a new
} lab to be significantly better than those specs, because the lab will
} (hopefully!) outlast the instruments you buy now. New instruments will, in
} all probability, have tighter specs than the present ones. Not only that,
} but not even the manufacturer KNOWS, for certain, that the instrument will
} work, even in an environment that meets the specs.. Only eating the
} pudding will provide the answer!
}
} This, by the way, is because the specifications are entirely empirical.
} There is no magic formula a manufacturer can use, plugging in various
} pieces of information about the microscope, which tells them the tolerable
} interference levels. They just pluck a figure from the air (well, based on
} the experience of *lots* of earlier installations, and their own lab
} instruments), and hope for the best. If your installation has
} difficulties, the next customer will find the specs tightened. Each site
} is slightly different, and can present some new twist of vibrational
} frequency, direction, or whatever, that can excite a previously unknown
} resonance in the microscope system. Alternatively, perhaps, your system
} may be subtly different from others (a new batch of wire for some of the
} springs in the stage, for example, changing the resonant frequencies), so
} it respondes differently.
}
} Some listers may not agree with my next comment, but in my experience,
} manufacturers will not abandon you if your site is a few percent out of
} spec - they will work with you, within reason, to get the instrument
} running well (it is bad publicity for them otherwise). The difference is
} that you may have to pay for extra amelioration, whereas if your site is in
} spec, they will (usually) pay.
}
} Intuitively, granite would be thought to be a good site - after all, don't
} we try to put pilings down to bedrock to get stable sites in other areas.
} However, it also transmits vibrations very well if any are induced.
} Sandstone, I am told, is much better, because it damps the vibrations much
} more. Any of them, I am certain, are better than the mud-filled
} saltmarshes on which MIT is built (hence my opening remark!).
}
} So where does that leave us, as users? Architects will ask you for the
} "Specifications" of the instruments you want to install, and will find the
} cheapest way of meeting them. In 1980, our EM site easily met the
} requirements for our EM300 and JEOL200CX. Surprise, surprise - it doesn't
} come close for a modern FEG-IVEM! On the other hand, given your location,
} is there much that can be done? Usually one tries to reach the bedrock,
} but in your case, the bedrock reaches you. It may be a case of what you
} have is all you can get. If there is too much vibration on your floor, you
} may have to live with what improvement an isolation system can provide
} (modern active ones are very effective).
}
} Get qualified, experienced engineers to perform your surveys. Your
} architects should have contact with people they have worked with in the
} past, and the microscope vendors certainly have such contacts. Don't
} forget acoustical interference, electromagnetic problems (magnetic fields,
} once generated, cannot be eliminated - only moved!). Ask for - nay, DEMAND
} - the best site money can buy. It will, in the long run, be a good
} investment for you lab.
}
} By the way, very little of the above represents quality scientific research
} - just a lot of strongly held subjective opinions formed over the years.
} Good lock!
}
} Tony Garratt-Reed
}
} At 01:30 PM 1/2/2002 -0500, Lesley S. Bechtold wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } } Happy New Year to everyone!
} } }
} } } We are setting up a totally new EM/LM lab - being built from the ground
} } } up. We have told our engineers that we need to have a vibration-free
} } } environment for optimum equipment operation. They would like to know
} } } exactly what vibration is tolerable and what isn't. Are there any
} } } standards or measurements out there that detail what limits can be
} } } tolerated and what can't?
} } }
} } } Thank you!
} } }
} } } Lesley
} } }
} } } Lesley S. Bechtold
} } } Supervisor, Biological Imaging
} } } The Jackson Laboratory
} } } 600 Main St.
} } } Bar Harbor, ME 04609
} } } 207-288-6191
} }
} } Lesley S. Bechtold
} } Supervisor, Biological Imaging
} } The Jackson Laboratory
} } 600 Main St.
} } Bar Harbor, ME 04609
} } 207-288-6191
} }



From daemon Sat Jan 5 09:04:18 2002



From: ykim39-at-uic.edu ()
Date: Sat, 5 Jan 2002 08:52:56 -0600
Subject: Ask-A-Microscopist: fluorescence emission queching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ykim39-at-uic.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
January 5, 2002 at 08:38:25
---------------------------------------------------------------------------

Email: ykim39-at-uic.edu
Name: YOUNGJUN KIM

Organization: UIC

Education: Graduate College

Location: Chicago, IL. USA

Question: Dear all;
I would like to know the principle of fluorescence emission queching.
If you know the information resource about this,
please tell me that(books, review paper)

Best wishs,

01-05-02

---------------------------------------------------------------------------


From daemon Sat Jan 5 12:25:09 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 5 Jan 2002 10:04:39 -0800
Subject: Re: cincinnati EM people

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

John -

Are you a MSA member? Members can search the membership list (on the
website) using any address criterion - including city. And anyone can look
at the list of Local Affiliate Society officers; there's an active one in
the Cincinnati area.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sat Jan 5 12:32:49 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 5 Jan 2002 10:26:56 -0800
Subject: Re: cincinnati EM people

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} hello all, any univ of cinci EM techs or EM lab managers here. i will be
} traveling to cinci on buisness at the univ of cinci. would be nice to know
} someone there, perhaps to talk with if i run into problems. email me back.
} John Hoffpauir
} Thomas Jefferson University
} Philadelphia Pa

John -

Are you a MSA member? Members can search the membership list (on the
website) using any address criterion - including city. And anyone can look
at the list of Local Affiliate Society officers; there's an active one in
the Cincinnati area.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sat Jan 5 13:56:32 2002



From: Nicholas Welham :      Nicholas.Welham-at-anu.edu.au
Date: Sun, 06 Jan 2002 06:48:45 +1100
Subject: LM- Unitron HHS heating stage instructions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Apologies to those who already have received this via Ask-a-microscopist.
I have just inherited a Unitron HHS heating stage but do not have any
instructions for its use. I have a good idea what to do but would prefer to
see if I can find a copy of the original instructions (Unitron don't have
them) before things go bang. Does anyone else have one of these with
instructions?
regards
Nick
___________________________________________________________
Dr. Nicholas Welham
Electronic Materials Engineering
Research School of Physical Sciences and Engineering
Australian National University
Canberra
ACT 0200, Australia

tel +61-2-6125-0520 fax +61-2-6125-0511

Nick's homepage: http://rsphysse.anu.edu.au/~njw109/



From daemon Sat Jan 5 17:10:56 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 05 Jan 2002 18:04:34 -0500
Subject: Re: service contract for microscope with Specialty Underwriters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yan Xin,
Is this Specialty Underwriters a third party service company or an
insurance company? This makes a big difference in how to approach the
problem. The name screams insurance company, not service company, in
which case you will still probably have JEOL doing the work but you will
now be a "billable" customer and go to the back of the class behind all
the "contract" customers. How time sensitive are you? Also, if this
is an insurance company, you will not get any direct technical help (a
potential time saver) and the field engineer coming in may not have
talked directly with you beforehand. so there may be misinformation that
causes further delays.

If it is actually a third party service company then you need
references. They could actually be a better deal, or a headache.
References, references references. And call them! Figure out what is
important to you and see how they stack up according to their own customers.

Good luck.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Yan Xin wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
} I would appreciate very much if anyone could provide me information on
} the pit falls to pursue microscope service contract with third party
} (in particular, the Specialty Underwriters) instead of Jeol. Inc.
}
} We have one Jeol SEM and one Jeol-2010 TEM to be put on contract this
} year. I have encountered difficulties in persuading our purchasing
} agent to go with Jeol Inc., because Specialty Underwriters has a
} quotation $1,000 lower. Because of the State Law, we need to provide
} evidence not to choose the lowest price vendor. I read comments
} concerning the pit falls of the service contract with third party on
} the server. But I would really need hard data.
}
} It will be great if anyone could help.
}
} Best Regards
} Yan Xin
}
}
} =======================================
} Yan Xin (Ph.D)
} Magnet Science & Technology
} National High Magnetic Field Laboratory
} Florida State University
} 1800 E. Paul Dirac Drive
} Tallahassee, FL 32310
} Tel: (850) 644 1529
} Fax: (850) 644 0867
} ========================================
}
}
}
}
}
}



From daemon Sun Jan 6 21:59:42 2002



From: kfdavis-at-pacbell.net ()
Date: Sun, 6 Jan 2002 21:38:54 -0600
Subject: Ask-A-Microscopist: LM prepared slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kfdavis-at-pacbell.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
January 6, 2002 at 19:30:47
---------------------------------------------------------------------------

Email: kfdavis-at-pacbell.net
Name: Kenneth Davis

Education: Undergraduate College

Location: Rocklin, CA, USA

Question: Recently purchased a stereo binocular zoom
microscope (7-35 with 10x eyepieces) for our
grandson. Can you recommend a source or supplier
of professionally prepared slides, on a range of
subjects, that would complement the instrument
and captivate a Middle School student?

Thanks in advance
KF & JA Davis

---------------------------------------------------------------------------


From daemon Mon Jan 7 02:47:23 2002



From: Mustapha Jouiad :      jouiad-at-lmpm.ensma.fr
Date: Mon, 07 Jan 2002 09:31:29 +0100
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe


--------------------------------------------------------
DR. Mustapha JOUIAD

ENSMA/LMPM
Teleport 2, 1 Ave. Clément Ader, Futuroscope-Chasseneuil 86960
Tel. 33 (0) 5 49 49 82 09
Fax. 33 (0) 5 49 49 82 38
Email. jouiad-at-lmpm.ensma.fr
Web. www.lmpm.ensma.fr
[]
--------------------------------------------------------


From daemon Mon Jan 7 05:38:46 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 7 Jan 2002 11:28:36 +0000
Subject: gold removal from SEM specimens

Contents Retrieved from Microscopy Listserver Archives
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Dear All

I have been asked for methods of removing the gold sputtered
coating from
polished geological specimens that have been used as SEM
specimens. I have suggested washing with mercury, or alkaline
sodium cyanide solution, or ammonium thiocyanate solution.

Are there any other methods which would be preferable?

Best wishes, and Happy New Year

Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Mon Jan 7 08:54:54 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 7 Jan 2002 08:46:05 -0600
Subject: service contract for microscope with Specialty Underwriters

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
} From: Yan Xin [mailto:xin-at-magnet.fsu.edu]
Sent: Thursday, January 03, 2002 3:13 PM
To: Microscopy-at-sparc5.microscopy.com

I believe that Specialty Underwriters is an insurance (or "service
management") company. As Ken Converse has said, if they are third-party
service providers, then it's a different story and please disregard the
following:

Our experience with insurance companies has been absolutely miserable. At
one point it took us from April to December simply to get a preventive
maintenance visit scheduled on one machine. One insurance company declared
bankruptcy, costing a service provider a LOT of money. Our second insurance
company seems to be very responsible about paying its bills, but we have
definitely been put at the end of the line for service by service providers.

Through some recent discussions (they should be archived by Nestor in the
MSA site), I have been educated to see that this is a combination of several
factors. One is that service providers must ethically give priority to
those holding service contracts with them. Another is that service
providers are very reluctant to deal with ANY insurance company after one
insurance company has burned them for a lot of money. Yet another is that
field service engineers are spread very thin (see reason #1 above).
Finally, I believe that insurance companies are simply not set up to deal
with instruments like electron microscopes. Save them for centrifuges and
elevators.

I strongly (put "strongly" in boldface type and underline it) recommend that
you go with the JEOL contract. The $1000 you save will be nothing in
comparison with the headaches you will have, if our experience is any
indication. When we were on OEM contracts, we had no problems. Hopefully,
we will be in that happy state again in the near future.

(I have no financial, emotional, political, or blood ties with any OEM or
insurance company, by the way.)

I would be happy to discuss this more with you, if you like.

Good luck,

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/






Hi,
I would appreciate very much if anyone could provide me information on the
pit falls to pursue microscope service contract with third party (in
particular, the Specialty Underwriters) instead of Jeol. Inc.

We have one Jeol SEM and one Jeol-2010 TEM to be put on contract this
year. I have encountered difficulties in persuading our purchasing agent
to go with Jeol Inc., because Specialty Underwriters has a quotation $1,000
lower. Because of the State Law, we need to provide evidence not to choose
the lowest price vendor. I read comments concerning the pit falls of the
service contract with third party on the server. But I would really need
hard data.

It will be great if anyone could help.

Best Regards
Yan Xin


=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Mon Jan 7 10:17:59 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 7 Jan 2002 08:09:11 -0800
Subject: Re: Ask-A-Microscopist: LM prepared slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Email: kfdavis-at-pacbell.net
} Name: Kenneth Davis
}
} Education: Undergraduate College
}
} Location: Rocklin, CA, USA
}
} Question: Recently purchased a stereo binocular zoom
} microscope (7-35 with 10x eyepieces) for our
} grandson. Can you recommend a source or supplier
} of professionally prepared slides, on a range of
} subjects, that would complement the instrument
} and captivate a Middle School student?
}
} Thanks in advance
} KF & JA Davis
}
} ---------------------------------------------------------------------------
To answer your specific question, get a copy of the catalog from a large
supplier, such as Carolina Biological or Flinn Scientific. But if you
really want to "captivate a Middle School student", encourage him to
prepare his own samples. Please look at the MICRO bibliography for a
reviewed listing of books, videos, and CD-ROMs that will help. Don't miss
the "Eye of the Cyclops" middle school video series, prepared by a
naturalist-photographer who lives just a few miles from you, in Loomis.
And since the inverted image of a compound scope frustrates beginners, I
suggest you get the "Scopemaster" CD. Here's an updated listing that will
appear online soon:
-----------------------------
Neuronware 1997 Scopemaster Neuronware, 15 Madison Ave, Toronto,
Ontario M5R 2F2, Canada. For Mac or Windows. $70 - $75 from 3 U.S.
sources: Clearvue, 800-253-2788, Flinn Scientific, 800-452-1261, and
Sargent Welch, 800-727-4368 (New ordering information)
An interactive microscope teaches the use of the controls of a
compound microscope: The user can select three objectives, adjust the
substage diaphragm, and use coarse and fine focus. Advice on microscope
use appears if a mistake is made; if the advice is ignored, the high power
objective even breaks with a resounding "crack" if the slide hits it!
Slides must be centered on the stage in a realistic way that makes the
inverted image of the compound microscope understandable; it will be
nonthreatening, nondestructive practice for a beginner. Ten sets of nine
slides each (mostly biological) are included, each with its own
well-written reference book; the goal is specimen identification. The
images are good color light micrographs; each can be viewed full-screen
after the microscope is in focus, or all can be reviewed quickly in
"teacher" mode. There is a self-test, and a printable test for class use.
More information (and a downloadable update for Windows) is available on
the web at www.snap.ca/neuronware/index.htm. Middle and high school.
RECOMMENDED
---------------------------


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon Jan 7 12:20:24 2002



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Mon, 7 Jan 2002 18:16:10 -0000
Subject: Gold removal with KI/I2

Contents Retrieved from Microscopy Listserver Archives
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After the signature there is a collection of replies and search results
when this came up a month or two ago.

+-----------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+-----------------------------------------+
Phone:
{direct line +44 (0) 118 9318572
{University internal extension 7867
Fax: +44 (0) 118 9750203
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+-----------------------------------------+

(a) Recipe 1
4.6 g potassium iodide 1.3 g Iodine 100 ml DI water

Recipe 2
12.5 g potassium iodide 6.25 g iodine DI water to make 40 mls.

(b) 100 grams KI 60 grams I2 100 ml of DI water: dissolve all
together and add to 900 ml of Methanol.

and another version uses

(c) Mix 3 grams of potassium iodide and 5 grams of iodine in a beaker
with 50 ml of water.

b and c seem to have the correct molar ratio of KI to I2 (with I2
slightly in excess?) and I think one of these is the best to go for.





From daemon Mon Jan 7 12:55:29 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Mon, 7 Jan 2002 12:34:45 -0600
Subject: Collagen diameter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listies,

I have a query??????????????????

I'm imaging (TEM) collagen in primate and avian skin. I've found that the
diameter varies from 50 nm to 200 nm within the same "bundle" of collagen.
Is this typical?

Or, am I possibly having fixation / osmilarity problems???????????



Tim Quinn
University of Kansas
Research Assistant
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu


From daemon Mon Jan 7 14:14:45 2002



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Mon, 07 Jan 2002 12:06:22 -0800
Subject: Pics of embedding, sectioning, etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I am trying to track down some pictures of the embedding and sectioning
process to show to students in an introductory cellular biology class. We
want to demonstrate the process of acquiring the sample, processing,
infiltrating, embedding, and sectioning. We would especially like a
picture of the sections coming off the knife and floating as a ribbon on
the water. The instructor would like color photos, web quality for his web
based lecture notes. Any help, pointers to any specific sites, would be
appreciated.





Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Mon Jan 7 14:46:30 2002



From: Caroline Miller :      camiller-at-creighton.edu
Date: Mon, 07 Jan 2002 14:41:46 -0600
Subject: JB-4 Microtomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Are JB-4 microtomes still made and where can we get supplies for them,
such as chucks? Thanks, Caroline Miller



From daemon Mon Jan 7 15:35:47 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Mon, 07 Jan 2002 12:40:53 -0800
Subject: Re: gold removal from SEM specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris:

A similar question arose on another listserver, although it was removing
a 2 mciron gold metallizton from GaAs. The responses from there were
as follows:

Response 1
"Greetings!

It is possible that a cut or buffered aqua regia might do the trick. I
have had some success with the recipe below on Au
metallization at M2 and above on GaAs dice. The interlayer dielectric
was silicon dioxide, so there was not a lot of seepage into the
die substrate layer - this, more than the recipe, might have kept the
damage down. Assuming SiO2 as the ILD on all layers of your
part, this might still work:

50 ml deionized water

30 ml HCl

20 ml HNO3

Swirl or agitate the part in the solution for five minutes. Inspect and
repeat once if needed.

One minute rinse in deionized water, followed by 30 seconds rinse in
acetone. Dry under heat lamp to minimize surface staining.

As always, try this on a practice part first in case this does not
buffer the reaction with GaAs enough.

Good Luck !

Regards,

Carl Nail
National Semiconductor
Carl.Nail-at-nsc.com"

Response 2
"A solution of potassium iodide and iodine in water is a decent gold
etch. I have no idea how it would affect GaAs, but I suspect it would
be less destructive than Aqua Regia.

Alan Street
alan-at-irsi.com"

I hope this helps!

David

Chris Jeffree wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
}
} I have been asked for methods of removing the gold sputtered
} coating from
} polished geological specimens that have been used as SEM
} specimens. I have suggested washing with mercury, or alkaline
} sodium cyanide solution, or ammonium thiocyanate solution.
}
} Are there any other methods which would be preferable?
}
} Best wishes, and Happy New Year
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Mon Jan 7 16:02:44 2002



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Mon, 07 Jan 2002 15:54:05 -0600
Subject: oil soluble fluorochrome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
I have a user who wishes to study oil/water emulsions trapped in a
cellulose membrane using confocal microscopy. He wishes to visualize
trapped oil droplets by labeling them with an oil-soluble fluorescent
dye. It appears that Oil Red O works to a degree, however it doesn't hold
up well under the laser. Perhaps Nile Red would be a better
alternative? The membrane yields significant background so any suggestions
for selectively quenching autofluorescence from regenerated nitrocellulose
would be greatly appreciated. I welcome any suggestions for oil soluble
fluorochomes. Thanks in advance.
Cheers,
Karl G.


_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Mon Jan 7 16:14:57 2002



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Mon, 07 Jan 2002 16:10:56 -0600
Subject: Cancer research

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,
Can anyone who knows or is doing research on adenocarcinoma of the
lung (non-small cell) please contact me off the list for some
references? Thank you so much!




Tracey M. Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
Ames, IA 50011-1020
p. 515.294.3872
f. 515.294.1337



From daemon Mon Jan 7 17:18:58 2002



From: Sklyarov :      andskl-at-csd.uwm.edu
Date: Mon, 07 Jan 2002 16:17:03 -0800
Subject: EDX choice

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

We are very close to buy a new EDX System (instead of old HNU) for our
Topcon SEM. Following a bid process we should choose between PGT and
EVEX systems (both include digitized image subsystem). Price is about
the same for both although PGT offers a system with Be window (and thus
"Na and up") and EVEX - a system with a low element detector. I should
note that our Topcon SEM does not have an intermediate (or "prep")
chamber, and therefore the system will be open to atmosphere during a
sample change. How it might effect a low element detector performance?
How reliable those systems (EVEX and PGT). Shortly, how often did you
have problems with those systems and vendors?
All replies will be accepted with the "great thanks".

Andrey Sklyarov,
Advanced Analysis Facility
University of Wisconsin-Milwaukee
andskl-at-csd.uwm.edu
414/229-6692



From daemon Mon Jan 7 18:08:48 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 07 Jan 2002 16:04:28 -0800
Subject: Re: gold removal from SEM specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've worked on the same problem but with Au/Pd
on microchips. I use Ar plasma etching by setting
my Anatech Hummer VII to ETCH. I etch at 10%
power setting at 100mT vacuum. The trick is to
know when to stop etching. This is solved by plating
or coating a #1 cover slip the same as is the specimen.
Then, put both in the chamber and etch until the cover
slip is clear.

Use a low power and be prepared to etch for a LONG
time (perhaps an hour). Biological specimens might be
able to handle higher power settings than microchips.
High power for an IC will blow runners and poly. Try
a sacrificial specimen first, before committing your
actual specimen.

gary g.


At 03:28 AM 1/7/2002, you wrote:

} Dear All
}
} I have been asked for methods of removing the gold sputtered
} coating from
} polished geological specimens that have been used as SEM
} specimens. I have suggested washing with mercury, or alkaline
} sodium cyanide solution, or ammonium thiocyanate solution.
}
} Are there any other methods which would be preferable?
}
} Best wishes, and Happy New Year
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================



From daemon Mon Jan 7 18:42:47 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 07 Jan 2002 16:32:08 -0800
Subject: Re: gold removal from SEM specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chris,
I believe the best way is to lightly re-polish at the finest grit size used
for the original polish. This may not remove all the gold.
At 11:28 AM 1/7/02 +0000, you wrote:

} Dear All
}
} I have been asked for methods of removing the gold sputtered
} coating from
} polished geological specimens that have been used as SEM
} specimens. I have suggested washing with mercury, or alkaline
} sodium cyanide solution, or ammonium thiocyanate solution.
}
} Are there any other methods which would be preferable?
}
} Best wishes, and Happy New Year
}
} Chris
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Mon Jan 7 20:45:06 2002



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Mon, 7 Jan 2002 21:37:55 -0500
Subject: FL Society for Microscopy meeting in March

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mark you calendars!

The FL Society for Microscopy will be meeting with the FL AVS on March
11-12, 2002 at the University of Central Florida

Invited Speakers - Physical Sciences

David Williams, Lehigh Univ.
David Joy, U. Tennessee
David Field, Washington State Univ.
Molly McCartney, Arizona State Univ.
Leonid Chernyak, University of Central Florida

Invited Speakers - Biological Sciences

Eugene Goldberg , University of Florida
Laurie Gower, University of Florida


There will also be a FIB session and FIB users group meeting on March 12.
For more information on submitting an abstract or participating in the
users group meeting please contact either

Lucille Giannuzzi, lag-at-mail.ucf.edu

or

Fred Stevie, fred_stevie-at-ncsu.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Mon Jan 7 20:49:45 2002



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Mon, 7 Jan 2002 21:44:39 -0500
Subject: Physical Sciences Specimen Prep Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


UCF TEM Specimen Preparation Short Course - 2002

A Short Course With Emphasis on Recent Innovations in Tools and Methods

(Including tripod polishing, ion milling, and FIB techniques.)

Instructors:
Ron Anderson, IBM (retired);
Fred Stevie, NC State;
Lucille Giannuzzi, UCF


At the University of Central Florida (prior to the FL AVS/FL Society for
Microscopy Meeting)
Orlando, FL

Friday, Saturday and Sunday, March 8,9,10, 2002


for registration information please contact: Lucille Giannuzzi,
lag-at-mail.ucf.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Tue Jan 8 00:11:30 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Mon, 07 Jan 2002 21:57:41 -0500
Subject: Re: basic bio TEM questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 1/3/02 12:29 PM, Mick Thomas at mgt3-at-ccmr.cornell.edu wrote:
}
} I was recently asked for some help with TEM of biological specimens
} (cells). Since I work in the materials side of microscopy, I could answer
} some questions but not all. I would appreciate any help with the following
} questions:
}
} 1) Specimen thickness: How thick can a cell structure be and still be able
} to resolve 20nm features at 100keV? 300keV?
} 2) Beam damage: What sort of damage typically occurs to such specimens and
} are cold stages required?
} 3) Charging: Do bio specimens need to be coated for TEM (to dissipate the
} charge) and if so what kind of coating is used and how thick?
} 4) Contrast: Are such specimens typically stained and if so what sort of
} staining is used?
}
} I realize many volumes could probably be written on each of the above, but
} any help pointing us in the right direction would be very much appreciated.
}
} Thanks,
}
Dear Mick,
Since no experts have answered you, I'll take a shot.

1) At 100 keV, sections are typically no more than ~100 nm; at 300 keV,
roughly 250-500 nm sections can be examined. I don't know the level of
resolution for these thicknesses, so I can't be sure that 20 nm features can
be resolved, but this seems a modest requirement. Furthermore, these
thicknesses depend on the nature of the cells--the limitation at 300 keV is
likely to be the overlap of features more than transparency of the
section--the staining procedure, and other parameters (such as if you wish
to take a tomographic series, where a high tilt gives a larger effective
thickness).

2) If the cells are fixed, embedded in epoxy, and stained, the specimens
are usually not badly damaged by radiation (i.e., the loss of resolution
after irradiation is usually not significant), so cold stages are not needed
for these conventionally-prepared specimens; cells not fixed, embedded, and
stained are very susceptible to radiation damage, and a cold stage is
essential. Again, if tomography is needed, there will be 50 to 100
exposures of the same area, so the effects of radiation damage will be
greater by that factor, and will not be the same in each exposure.

3) Usually the formvar-covered grid is carbon coated to eliminate charging.

4) Yes. Heavy-metal stains are the most common: uranyl acetate with or
without lead citrate, osmium tetroxide (as both a stain and lipid fixative),
and phosphotungstic acid are the ones I've heard of.

Many volumes have, indeed, been written on these--Hyatt has a whole series,
and Bozzola and Russell have written a book on biological EM. I apologize
to all the other authors on the Microscopy List and elsewhere whom I have
not cited due to ignorance of their work. Good luck.
Yours,
Bill Tivol



From daemon Tue Jan 8 00:29:45 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 08 Jan 2002 01:25:37 -0500
Subject: Removal of gold layer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Chris Jeffree wrote:
====================================================
I have been asked for methods of removing the gold sputtered
coating from
polished geological specimens that have been used as SEM
specimens. I have suggested washing with mercury, or alkaline
sodium cyanide solution, or ammonium thiocyanate solution.

Are there any other methods which would be preferable?
===================================================
I would be a bit worried about some of these liquid suggestions possibly
dissolving out or reacting with species in the geological sample.

Could one not

a) put the sample into a sputter coater with etch mode (I realize not all
sputter coaters have the etch mode, but this might be one of the few good
applications for it) and literally sputter off the gold layer? This is not
a brand-specific suggestion, just about any sputter coater with etch should
do it.

b) expose to an oxygen plasma in a reactive plasma etcher such as the SPI
Plasma Prep™ II plasma etcher. I have mentioned this before, and that the
chemistry we have never figured out, but after about thirty minutes, most
gold layers seem to somehow get removed. Unless there were organics in the
geological sample, the exposure should not change the sample. However, the
oxygen plasma will start to etch away the embedding plastic, which might not
necessarily be a bad thing because that will permit a different kind of a
view of the polished geological sample ( you can now look into the voids
that have been opened up).

Disclaimer: SPI Supplies manufactures the Plasma Prep™ II plasma etcher for
this kind of application.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Tue Jan 8 01:44:44 2002



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Tue, 08 Jan 2002 08:47:07 +0100
Subject: Re: Collagen diameter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I can't comment on primate and avian specifically although I guess since we
share so much genome and collagen is so highly conserved the story should
be similar.

Type III would be the thinner of what you are seeing, Type I would be
thicker but not 200nm - more like 80nm if memory serves. Any possibility of
elastin???

Have you seen this 'problem' before??

Nadolig Llawen a Blwddyn Newydd Dda / God Jul och Gott Nytt År / Merry
Christmas and a Happy New Year

Med vänliga hälsningar/With best wishes

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734 Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi,
Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Dept of Biomedical Laboratory Science,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Tue Jan 8 08:38:56 2002



From: Chen Chen :      cche1-at-mail.jhmi.edu
Date: Tue, 08 Jan 2002 09:34:29 -0500 (EST)
Subject: knock-out mice

Contents Retrieved from Microscopy Listserver Archives
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Hi, everyone. Does anybody know which company can do knock-out mice?

Thank you.

Chen Chen

Department of Biological Chemistry
The Johns Hopkins University
School of Medicine
725 N. Wolfe Street
Baltimore, Maryland 21218



From daemon Tue Jan 8 08:43:24 2002



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Tue, 8 Jan 2002 08:46:25 -0500
Subject: RE: service contract for microscope with Specialty Underwriters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Yan,

Specialty Underwriters is an insurance company. In my previous position in
a hospital pathology lab; we tried "Specialty". It wasn't so bad with our
more general equipment, however when it came to the EM scopes; it was a
different story. I must say, I agree with Randy's comments. We were always
the last on the priority list, no matter what the emergency was. It didn't
matter that we were a hospital and that patient care was dependent on our
work. Also, you have to be careful how the contract is written. For
example: 1 PM or 2 for the year; are all parts included or just some; and
how are emergency visits scheduled? Make sure you have all the details of
the contract. Personally, it was a bad experience and certainly not worth
the financial savings.

If you do decide to go with "Specialty", then at least have a good PM from
your current service provider prior to the end of the contract.

Also, one final point, which you should investigate. If there is a lapse in
coverage, some companies will charge a "qualifying or inspection fee" prior
to offering a service contract. I had that happen with Zeiss when we wanted
to switch back to them after having a contract with Specialty. Even though
Zeiss provided the service for the duration of our Specialty contract,
because we didn't have a contract with them exclusively, there was no
guarantee that anyone else hadn't worked on the scope. So for their
protection, they required an instrument inspection at our cost. Of course,
we learned this the hard way. No one warned us of this. So please, talk to
JEOL about this potential cost. If "Specialty" doesn't work out and you
should want to go back to JEOL...Are there any hidden costs to re-sign with
JEOL?

Good Luck with your decision.
Jackie

Jackie Garfield
Electron Microscopist
Lifecell Corporation
One Millennium Way
Branchburg, NJ 08807
E-mail: jgarfield-at-lifecell.com



From daemon Tue Jan 8 09:26:11 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 08 Jan 2002 10:20:25 -0500
Subject: Re: Pics of embedding, sectioning, etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick Harris wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Colleagues,
}
} I am trying to track down some pictures of the embedding and sectioning
} process to show to students in an introductory cellular biology class. We
} want to demonstrate the process of acquiring the sample, processing,
} infiltrating, embedding, and sectioning. We would especially like a
} picture of the sections coming off the knife and floating as a ribbon on
} the water. The instructor would like color photos, web quality for his web
} based lecture notes. Any help, pointers to any specific sites, would be
} appreciated.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} 530 754 7536 fax
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu

Dear Rick:

Several books on EM sample prep have such photos, but putting them on a
website might be copyright infringment? Does your University have a media dept.
that could take photos for you? Or a photography dept.? Getting good shots of
sections floating in the boat is NOT easy, the sections must be close to
parallel to the film plane for optimum focus, fluorescent lighting makes
correct color balance difficult to achieve, etc.
The movie "The Andromeda Strain" has cinemacrophotography of thin
sectioning in progress, also a nice shot of an RCA/ForgeFlo 4C microscope.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Jan 8 09:41:55 2002



From: Bradley Starcevich :      starcharuski-at-mysun.com
Date: Tue, 08 Jan 2002 07:33:22 -0800
Subject: TEM

Contents Retrieved from Microscopy Listserver Archives
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Greetings,

I have a Zeiss TEM (EM-9S-2) available. Needs
chiller and vacuum pump. Many extra spare parts.
Complete with manuals and schematics. First
$1,000.00 takes it. Buyer responsible for
shipping.

Bradley K. Starcevich
Microscopist_1-at-lycos.com


Bradley K. Starcevich
http://www.starcevich.org



From daemon Tue Jan 8 10:29:34 2002



From: Stanley L. Flegler :      flegler-at-pilot.msu.edu
Date: Tue, 08 Jan 2002 11:22:06 -0500
Subject: Osmium Plasma Coater Users

Contents Retrieved from Microscopy Listserver Archives
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Are there any users of osmium plasma coaters that would be willing to share
their experiences? I am especially interested in knowing the following:

1. How long have you had your unit?
2. Have you had any problems with it?
3. Are you happy with the coatings that you obtain?
4. What type of samples do you typically coat?
5. Have you had opportunity to compare your coatings with other high
resolution coatings?

Any other comments you would care to share would be appreciated.

Stanley L. Flegler, Director
Center for Advanced Microscopy
Michigan State University


From daemon Tue Jan 8 10:29:35 2002



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Tue, 08 Jan 2002 08:22:29 -0800
Subject: Re: Pics of embedding, sectioning, etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for your replies. I was approached at noon for these photos by a
faculty member notorious for waiting until the last minute. He wanted the
pics by 5pm. After searching several texts and not finding what he needed
I posted my note to the list then I got out the digital camera and shot the
pictures myself. I was able to get a pic of a ribbon of sections by
shooting thru the eyepiece on the dissecting scope on the
ultramicrotome. I would not consider the picture to be excellent but it
was pretty good. The other stuff was easy and he got what he needed.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Tue Jan 8 11:59:25 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 8 Jan 2002 09:51:45 -0800 (PST)
Subject: Re: Collagen diameter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have looked at human collagen diameters in the reticular dermis and the
fibers tend to run from 80 - 100 nm + or - about 8 nm in a nice bell
curve.

Bob

On Mon, 7 Jan 2002, Quinn, Tim Lee wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear listies,
}
} I have a query??????????????????
}
} I'm imaging (TEM) collagen in primate and avian skin. I've found that the
} diameter varies from 50 nm to 200 nm within the same "bundle" of collagen.
} Is this typical?
}
} Or, am I possibly having fixation / osmilarity problems???????????
}
}
}
} Tim Quinn
} University of Kansas
} Research Assistant
} Natural History Museum and Biodiversity Research Center
} Dyche Hall Room 414
} Lawrence, KS 6604-2454
} 785-864-4556
} tquinn-at-ku.edu
}
}



From daemon Tue Jan 8 13:04:01 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Tue, 8 Jan 2002 13:55:07 -0800
Subject: agfa photographic paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
does anyone have a favorite place to buy Agfa RC photographic paper
(multi-contrast and #4).
happy new year to everyone,
Beth

***************************************************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
***************************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Tue Jan 8 13:16:03 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 8 Jan 2002 13:10:22 -0600
Subject: RE: service contract for microscope with Specialty Underwriters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I concur with everything Jackie says. We also will be expected to recertify
any scope that was not covered by OEM contracts for a period, if we want to
return to our old service contracts (which we do!), even if the OEM service
engineers were the only ones to touch the scope. This recertification will
cost about $1500 per microscope, unless you have two or more of the same
make that can be done in a single visit.

I repeat my first advice: stay with an OEM contract. Contracts with
third-party service providers are also an option, but have a contract with
somebody who recognizes you as a priority customer they deal with directly.


Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Jacqueline D. Garfield [mailto:JGarfield-at-lifecell.com]
Sent: Tuesday, January 08, 2002 7:46 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Dear Yan,

Specialty Underwriters is an insurance company. In my previous position in
a hospital pathology lab; we tried "Specialty". It wasn't so bad with our
more general equipment, however when it came to the EM scopes; it was a
different story. I must say, I agree with Randy's comments. We were always
the last on the priority list, no matter what the emergency was. It didn't
matter that we were a hospital and that patient care was dependent on our
work. Also, you have to be careful how the contract is written. For
example: 1 PM or 2 for the year; are all parts included or just some; and
how are emergency visits scheduled? Make sure you have all the details of
the contract. Personally, it was a bad experience and certainly not worth
the financial savings.

If you do decide to go with "Specialty", then at least have a good PM from
your current service provider prior to the end of the contract.

Also, one final point, which you should investigate. If there is a lapse in
coverage, some companies will charge a "qualifying or inspection fee" prior
to offering a service contract. I had that happen with Zeiss when we wanted
to switch back to them after having a contract with Specialty. Even though
Zeiss provided the service for the duration of our Specialty contract,
because we didn't have a contract with them exclusively, there was no
guarantee that anyone else hadn't worked on the scope. So for their
protection, they required an instrument inspection at our cost. Of course,
we learned this the hard way. No one warned us of this. So please, talk to
JEOL about this potential cost. If "Specialty" doesn't work out and you
should want to go back to JEOL...Are there any hidden costs to re-sign with
JEOL?

Good Luck with your decision.
Jackie

Jackie Garfield
Electron Microscopist
Lifecell Corporation
One Millennium Way
Branchburg, NJ 08807
E-mail: jgarfield-at-lifecell.com



From daemon Tue Jan 8 15:21:35 2002



From: Tamara Howard :      thoward-at-unm.edu
Date: Tue, 8 Jan 2002 14:13:37 -0700 (MST)
Subject: Xe lamp turning off

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are having a minor disagreement here so I thought I'd consult the
experts: Do the same cautions apply when turning OFF Hg and Xe lamps, as
far as protecting computers from a pulse, as when turning the lamps ON?

Sorry if some of you see this twice; I'm cross-posting to both the
Microscopy and Confocal servers.

TIA

Tamara

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|




From daemon Tue Jan 8 18:33:09 2002



From: Rotole, John A. :      John.Rotole-at-ispat.com
Date: Tue, 8 Jan 2002 18:23:11 -0600
Subject: WDX measurement uncertainty

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning everyone,

I have recently inherited a Joel JXA-8600 EPMA and the quality system
issues associated with it. Can anyone give suggestions as how best
to characterize the measurement uncertainty of WDX measurements? The
goal is for our laboratory to comply with measurement uncertainty
requirements specified in section 5.4 of ISO/IEC 17025. Any
suggestions as to how others have accomplished this task would be
greatly appreciated.

Thanks!

John

John A. Rotole, Ph.D.
Project Engineer
Coatings & Surface Technology
Ispat Inland Product Research
3001 E. Columbus Drive
East Chicago, Indiana 46312
Tel: 219-399-6308
Fax: 219-399-6562


From daemon Wed Jan 9 07:27:33 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 9 Jan 2002 07:09:45 -0600
Subject: RE: Xe lamp turning off

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


While there can be some "inductive kick-back" from a transformer when the
load is removed quickly, it is not the same as turning on a high pressure
xenon lamp. To light a Xe lamp, ionization is usually initiated by a rather
high voltage pulse. After ionization, the Xe becomes a good conductor
instead of an insulator. At that point the lower voltage, high current
portion of the Xe power supply takes over to sustain the lamp.

A well designed, filtered, etc. power supply *should not* feed back much
trash to the supply line - starting or otherwise. Can't speak to all
brands/designs... In a poorly shielded system there could be a significant
amount of radiated energy during Xe start-up, but even that should not
damage a PC unless rather tightly coupled.

Woody

} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} We are having a minor disagreement here so I thought I'd consult the
} experts: Do the same cautions apply when turning OFF Hg and
} Xe lamps, as
} far as protecting computers from a pulse, as when turning the
} lamps ON?
}
} Sorry if some of you see this twice; I'm cross-posting to both the
} Microscopy and Confocal servers.
}
} TIA
}
} Tamara
}
} |--------------------------------------------------|
} Tamara Howard
} Department of Cell Biology and Physiology
} University of New Mexico - Health Sciences Center
} Albuquerque, NM 87131
} thoward-at-unm.edu
} |--------------------------------------------------|
}
}
}


From daemon Wed Jan 9 08:37:47 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Wed, 9 Jan 2002 10:59:55 -0330
Subject: RE: WDX measurement uncertainty

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John writes ...

} I have recently inherited a Joel JXA-8600 EPMA and the quality system
} issues associated with it. Can anyone give suggestions as how best
} to characterize the measurement uncertainty of WDX measurements? The
} goal is for our laboratory to comply with measurement uncertainty
} requirements specified in section 5.4 of ISO/IEC 17025. ...

To me, section 5.4 is pretty darn vague ... but is does include a
reference to "Use of latest valid edition of standards" which is most times
difficult to adhere to unless your facility's adherence to 17025 is
extremely focussed. For example, I am not aware of any "valid edition" of
EPMA standards ... although many reference standards are well characterized.

However, I will be exploring the following wwwsite for "ACCREDITED
CALIBRATION & TESTING LABORATORIES" (EPMA), and its "UNCERTAINTY LINKS" for
EPMA references:
http://www.fasor.com/iso25/

genuinely ... michael shaffer :o)
Avalon Peninsula, Newfoundland



From daemon Wed Jan 9 09:30:25 2002



From: Peter Heimann :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Wed, 09 Jan 2002 16:23:04 +0100
Subject: EM-Film "AGFA ORTHO 25" ? equivalent successor available ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,
we are using for the last 16 years at our ZEISS transmission
electron microscope EM 109 (with transfiber plate) the
orthochromatic b/w roll film type 120 "AGFA ORTHO 25".
However, this film is not produced anymore. Has anybody found an
equivalent successor? Does anybody know an orthochromatic
"120" roll film?
Thank you for a short informal answer!
Peter Heimann
**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************


From daemon Wed Jan 9 14:18:21 2002



From: =?iso-8859-1?Q?=22Mirtha_Romano=2E_Lab=2E_Microscop=EDa_Electr=F3nica?=.=?iso-8859-1?Q?=22_=3Cmromano=40pasteur=2Eivic=2Eve=3E?=-at-ivic.ve
Date: Wed, 09 Jan 2002 16:08:32 -0400
Subject: Cell Culture Osteoblast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all:

I need to buy Cell Culture Osteoblast. Does anyone out there know
what companies sell this kind of cells?

Thanks in advance for your help.
Regards,

Mirtha Romano
Instituto Venezolano de Investigaciones Científicas
Centro de Microbiología y Biología Celular
Servicio de Microscopía Electrónica
Apartado 21827
Caracas 1020-A
Venezuela

mromano-at-ivic.ve





From daemon Wed Jan 9 20:25:30 2002



From: alan stone :      as-at-astonmet.com
Date: Wed, 09 Jan 2002 20:16:01 -0600
Subject: RE: WDX measurement uncertainty

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a problem for all ISO 17025 labs. We are A2LA accredited and have
to comply with this for all of our analytical procedures. Unless all labs
calculate their uncertainty budgets according to some common criteria, it
will be useless, time consuming and expensive exercise.

How do we calculate our uncertainty for something as subjective as grain size?

The whole idea sounds good, but its implementation was not thought out
prior to imposing it onto the labs.

My 2.0000 plus 0.0005/minus 0.0002 worth.





} John writes ...
}
} } I have recently inherited a Joel JXA-8600 EPMA and the quality system
} } issues associated with it. Can anyone give suggestions as how best
} } to characterize the measurement uncertainty of WDX measurements? The
} } goal is for our laboratory to comply with measurement uncertainty
} } requirements specified in section 5.4 of ISO/IEC 17025. ...
}
} To me, section 5.4 is pretty darn vague ... but is does include a
} reference to "Use of latest valid edition of standards" which is most times
} difficult to adhere to unless your facility's adherence to 17025 is
} extremely focussed. For example, I am not aware of any "valid edition" of
} EPMA standards ... although many reference standards are well characterized.
}
} However, I will be exploring the following wwwsite for "ACCREDITED
} CALIBRATION & TESTING LABORATORIES" (EPMA), and its "UNCERTAINTY LINKS" for
} EPMA references:
} http://www.fasor.com/iso25/
}
} genuinely ... michael shaffer :o)
} Avalon Peninsula, Newfoundland
}




From daemon Wed Jan 9 21:33:37 2002



From: Gordon Couger :      gcouger-at-couger.com
Date: Wed, 9 Jan 2002 21:26:38 -0600
Subject: Re: Xe lamp turning off

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


: }
: }
: } We are having a minor disagreement here so I thought I'd consult the
: } experts: Do the same cautions apply when turning OFF Hg and
: } Xe lamps, as
: } far as protecting computers from a pulse, as when turning the
: } lamps ON?
: }
: } Sorry if some of you see this twice; I'm cross-posting to both the
: } Microscopy and Confocal servers.
: }
: } TIA
: }
: } Tamara
: }
: } |--------------------------------------------------|
: } Tamara Howard
: } Department of Cell Biology and Physiology

The easy way to find out is to connect a fast recording digital oscilloscope
across the connection to the power mains and record what happens when you
turn on and off the light if you suspect the problems coming through the
power line. This should be easily controlled with the proper power
conditioner connected to the light power source. It is nothing more than a
large inductance with capacitors and other elements shorting the high
frequency and high voltage components to ground before they get on the
mains.

If you think the problems are being radiated through the air a spectrum
analyzer should show up any problems in that area. Proper shielding and
grounding should take care of any problems in this area.

In both cases a good short low impedance path to earth ground helps a great
deal. The ground for this should be physically separate from the ground in
the wiring but there should be no potential between the grounds. The should
be electrically connected. To get this to function correctly and meet
electrical code is beyond the scope of most electricians. A good radio
broadcast engineer would be the person I would ask to find some one local to
help or possibly one of the older faculty members in electrical engineering
that has worked with radio frequency transmitters.

Good luck
Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger




From daemon Thu Jan 10 07:36:40 2002



From: Teresa Flores :      tflore-at-lsuhsc.edu
Date: Thu, 10 Jan 2002 07:26:28 -0500
Subject: AGFA ORTHO 25 TEM Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter, our EM lab has an EM 109 Zeiss and we also encountered this problem
several years ago and resolved it by using:
Technical Pan Film 6415 (TP 120)
Cat # 74133
120mm, E. Kodak #151 1054
Ordered from:
Electron Microscopy Sciences
215-646-1566 Phone
SGK cck-at-aol.com
We use Dektol to develope the TP 120 and KodaFix to fix it.
Hope this helps.Teresa

Colleagues,
we are using for the last 16 years at our ZEISS transmission
electron microscope EM 109 (with transfiber plate) the
orthochromatic b/w roll film type 120 "AGFA ORTHO 25".
However, this film is not produced anymore. Has anybody found an
equivalent successor? Does anybody know an orthochromatic
"120" roll film?
Thank you for a short informal answer!
Peter Heimann




From daemon Thu Jan 10 10:02:32 2002



From: Kathryn Schubel :      schubelk-at-lafayette.edu
Date: Thu, 10 Jan 2002 10:53:09 -0500
Subject: wanted: charles supper precision measuring device

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in obtaining a Charles Supper precision measuring device.
If anyone has one for sale please contact me.

Thanks, KS

Kathryn Schubel
Assistant Professor
Department of Geology and
Environmental Geosciences
Lafayette College
Easton, PA 18042

(610) 330-5194 (phone)
(610) 330-5717 (fax)
schubelk-at-lafayette.edu

Spring 2002:

KAS
Visiting Assistant Professor
Department of Earth and Planetary Sciences
Johns Hopkins University
Baltimore, MD 21218

schubelk-at-lafayette.edu





From daemon Thu Jan 10 13:18:00 2002



From: robert.fowler-at-tdktca.com
Date: Thu, 10 Jan 2002 14:05:49 -0500
Subject: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,
Due to the fact that Polaroid will soon stop production of 667 B&W instant
film, I have an immediate need for a digital camera for my JEOL T220 A SEM.
Any help (vendors welcome) would be appreciated on or off listserver.
Budget is around 2 - 3k. I am down to my last few boxes, and although I
still can reorder I prefer not to................

TIA

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



From daemon Thu Jan 10 13:41:58 2002



From: Harry Walsh :      h_walsh-at-acs.org
Date: Thu, 10 Jan 2002 14:29:03 -0500
Subject: Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings, I am writing with the request that you include the following
announcement:

----------------------------------------------------------------------------
----------------------------------------------------------------------------
--------------------------------------
The American Chemical Society will be offering its popular short course,
Applied Optical Microscopy, on March 15-17, 2002, immediately preceding
PITTCON 2002 in New Orleans, LA. The course is designed for researchers,
technicians, and quality assurance and failure analysis scientists who need
to develop a strong foundation in optical microscopy or who wish to extend
their current capability in the field. A full course description can be
found in the online catalog at www.chemistry.org/shortcourses. Look under
"What's New" for the "ACS Short Courses at PITTCON 2002" catalog which is
downloadable as a pdf file. Or, contact the ACS at shortcourses-at-acs.org or
800-227-5558, extension 4508. The course registration fee is $1,095 for ACS
members and $1,195 for nonmembers.
----------------------------------------------------------------------------
----------------------------------------------------------------------------
---------------------------------------

Thank you for your help. Please feel free to contact me if you have
questions or need additional information.


********************************************************************
Harold G. Walsh
Department of Continuing Education
American Chemical Society
1155 Sixteenth Street, N.W.
Washington, DC 20036

Phone: 800-227-5558, extension 4507, or 202-872-4507
Fax: 202-872-6336
Email: h_walsh-at-acs.org

Visit our web site at www.chemistry.org/shortcourses
********************************************************************



From daemon Thu Jan 10 13:42:47 2002



From: evgenia.pekarskaya-at-exxonmobil.com
Date: Thu, 10 Jan 2002 14:37:46 -0500
Subject: sample prep/wire EDM vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I am looking for a wire EDM (spark cutter) for both slicing specimens and
drilling holes (TEM disks).
Ideally, I would like to buy a model of a small size (not an industrial
scale), that could be put on top of a bench.
I made a search on the Web but it was not very successful.
I would appreciate if somebody could share his/her experience and recommend
a vendor, preferably in the US.

Thank you very much.
Evgenia

**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355
e-mail: evgenia.pekarskaya-at-exxonmobil.com



From daemon Thu Jan 10 13:44:37 2002



From: S Keller :      swtkeller-at-yahoo.com
Date: Thu, 10 Jan 2002 11:39:53 -0800 (PST)
Subject: Service for ISI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:
There was a thread about ISI service. Who
is now responsible for their service?
Thanks in advance,
Sandra

__________________________________________________
Do You Yahoo!?
Send FREE video emails in Yahoo! Mail!
http://promo.yahoo.com/videomail/


From daemon Thu Jan 10 14:00:12 2002



From: David Spector :      spector-at-cshl.org
Date: Thu, 10 Jan 2002 14:54:37 -0500
Subject: Position Available: Biological Microscopy Core Manager

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cold Spring Harbor Laboratory on the north shore of Long Island, New
York is seeking an experienced and responsible Biological Microscopy
Facility Core Manager for the laboratory's state-of-the-art central
microscopy facility. The individual should have practical expertise
in transmission electron microscopy, confocal and widefield
fluorescence microscopy, and digital imaging. The successful
candidate will be involved in designing and carrying out experimental
protocols for users, training individuals in the use of various
microscopes, and aligning microscopes and keeping the facility
operating at an efficient and high level of productivity. Interested
individuals should send their resume, including a description of
their expertise and the names and addresses of 3 references to: Dr.
David L. Spector, email: spector-at-cshl.org
--
Dr. David L. Spector
Cold Spring Harbor Laboratory
One Bungtown Road
Cold Spring Harbor, New York 11724
Tel. (516) 367-8456
Fax (516) 367-8876
email: spector-at-cshl.org


From daemon Thu Jan 10 15:55:50 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 10 Jan 2002 16:47:44 -0500
Subject: sample prep/wire EDM vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ernest F Fullam http://www.fullam.com/ sells such a unit and so does South Bay Technology https://www.southbaytech.com/

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: "evgenia.pekarskaya-at-exxonmobil.com"-at-sparc5.microscopy.com
[mailto:"evgenia.pekarskaya-at-exxonmobil.com"-at-sparc5.microscopy.com]
Sent: Thursday, January 10, 2002 2:38 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Colleagues,

I am looking for a wire EDM (spark cutter) for both slicing specimens and
drilling holes (TEM disks).
Ideally, I would like to buy a model of a small size (not an industrial
scale), that could be put on top of a bench.
I made a search on the Web but it was not very successful.
I would appreciate if somebody could share his/her experience and recommend
a vendor, preferably in the US.

Thank you very much.
Evgenia

**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355
e-mail: evgenia.pekarskaya-at-exxonmobil.com



From daemon Thu Jan 10 17:43:58 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 10 Jan 2002 18:42:41 -0500
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
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Robert,
A "digital camera" is not going to be of any value to you. An SEM takes
pictures by scanning one dot at a time and that is how your Polaroid
film is exposed. You can, however, add a digital imaging system (active
or passive) that will capture your images. Unfortunately your budget is
not suited to that as they tend to run about 10k and up.

On the other hand, you seem to be in a materials oriented application.
Do you have an EDS system? They often have, or can be upgraded to,
digital imaging. This may be your best bet.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Hello Listers,
} Due to the fact that Polaroid will soon stop production of 667 B&W instant
} film, I have an immediate need for a digital camera for my JEOL T220 A SEM.
} Any help (vendors welcome) would be appreciated on or off listserver.
} Budget is around 2 - 3k. I am down to my last few boxes, and although I
} still can reorder I prefer not to................
}
} TIA
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}
}
}
}



From daemon Thu Jan 10 20:21:51 2002



From: Beverly Giammara :      giammara-at-bellsouth.net
Date: Thu, 10 Jan 2002 20:44:17 -0500
Subject: TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Friends and Colleagues,
To gain space, we have two Philips 201s for sale. They are serviced
and are in excellent condition. If you are interested in one or both,
please call or email Dr. Fred Roisen, Anatomical Sciences and
Neurobiology, University of Louisville School of Medicine, Louisville,
KY.
Telephone: 502-852-5165. The email is fjrois01-at-gwise.louisville.edu
Thank you very much.
Kind regards to all.
Beverly Giammara



From daemon Thu Jan 10 20:25:42 2002



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Fri, 11 Jan 2002 15:20:24 +1200
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robert

For a simple low cost digital image option try looking at ImageSlave
http://members.ozemail.com.au/~sbwisbey/imageslave.html

Ian

} Hello Listers,
} Due to the fact that Polaroid will soon stop production of 667 B&W instant
} film, I have an immediate need for a digital camera for my JEOL T220 A SEM.
} Any help (vendors welcome) would be appreciated on or off listserver.
} Budget is around 2 - 3k. I am down to my last few boxes, and although I
} still can reorder I prefer not to................
}
} TIA
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}
}



Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From daemon Thu Jan 10 21:06:30 2002



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Fri, 11 Jan 2002 13:59:59 +1100
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
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Hi Robert

Our facility also uses ImageSlaves for almost all our routine SEM
imaging, we are very happy with them.

Sally

Sally Stowe
ANU Electron Microscopy Unit
http://www.anu.edu.au/EMU/index.html


} } } "IAN HALLETT" {ihallett-at-hortresearch.co.nz} 01/11/02 02:20PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Robert

For a simple low cost digital image option try looking at ImageSlave
http://members.ozemail.com.au/~sbwisbey/imageslave.html

Ian

} Hello Listers,
} Due to the fact that Polaroid will soon stop production of 667 B&W
instant
} film, I have an immediate need for a digital camera for my JEOL T220 A
SEM.
} Any help (vendors welcome) would be appreciated on or off listserver.
} Budget is around 2 - 3k. I am down to my last few boxes, and although I
} still can reorder I prefer not to................
}
} TIA
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}
}



Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________



From daemon Thu Jan 10 22:19:45 2002



From: =?ks_c_5601-1987?B?v8DH9r/s?= :      oh0504-at-mail.kribb.re.kr
Date: Fri, 11 Jan 2002 13:21:35 +0900
Subject: a postdoctoral position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear

I am searching a postdoctoral position.

On my searching activity,
Randy Tindall, EM Specialist, Electron Microscopy Core Facility,University of Missouri, suggest me to send my email to the Microscopy Society of America listserver, which reaches many EM laboratories at universities and companies around the world and tell me this email address.


The followings are my letter of application for a postdoctoral position and my CV.


Dear



I am writing this E-mail to inquire about a postdoctoral position.

Throughout my academic and professional carrier, I have been quite well equipped with broad range of experimental techniques in biological electron microscopy.

(I have been responsibility of electron microscopy lab. for 10 years)

With your kind consideration, I would like to get further information about postdoctoral training and availability of financial support at your lab.

Thank you in advance for your deep concern at my scientific interest and further advanced career.



Sincerely yours





Hyun Woo Oh

Tel: +82-42-860-4255

Fax: +82-42-860-4677

E-mail: ohwoo-at-mail.kribb.re.kr






Curriculum Vitae

Personal Information



Name: Hyun Woo Oh

Sex: Male

Birth date: May 4, 1963

Family relation: Married, one son and one daughter

Birthplace: (Seoul)Korea



Present Address



Electron microscopy Lab,

Korean Collection for Type Cultures,

Korea Research Institute of Bioscience and Biotechnology,

Eundong 52 Yusong, Taejon, Korea

Tel: 82-42-860-4255

Fax: 82-42-860-4677

E-mail: ohwoo-at-mail.kribb.re.kr



Education



1982 - 1986 Seoul National University B.S. Major on Entomology

1986 - 1990 Seoul National University M.S. Major on Entomology

1994 - 1999 Seoul National University Ph.D. Major on Entomology




Techniques


Maintain and operate all aspects of transmission electron microscopy, scanning electron microscopy and dark room works.

- Fix, embed, section, stain biological samples for transmission electron microscopy.

- Biological sample preparation for scanning electron microscopy.

- Examine and photograph thin sections using an electron microscope.

- Develop film and print micrographs.

- Prepare micrographs for publication.

- Make formvar or carbon coated grids.

- Order supplies and maintain inventory.

- Direct and occasionally perform the procurement and preparation of tissues(plant, animal), tissue culture specimens, microorganisms(bacteria, fungi, etc.) and other specimens of biological samples.


Recent Journal Articles



Lee, I.H., Y.H. JE, J.H. Chang, J.Y. Roh, H.W. Oh, S.G. Lee, S.C. Shin and K.S. Boo (2001) Isolation and characterization of a Bacillus thuringiensis ssp. kurstaki strain toxic to Spodoptera exigua and Culex pipens. Current Microbiology 43:284-287



Hong, S.G., J. Chun, H.W. Oh and K.S. Bae (2001) Metschnikowia koreensis sp. nov., a novel yeast species isolated from flowers in korea. Int J Syst Evol Micrbial 51:1927-1931


Park, S.J., H.W. Oh, Y.N. Youn and H.Y. Park (2001) Structure of antennal sensilla on the adult asian ladybird, Hamonia axyridis Pallas(Coleoptera: Coccinellidae). Korean J. Electron Microscopy 31:91-99



Moon, E.Y., H.W. Oh, P.J. Maeng and K.S. Bae (2001) Identification of Enteric bacteria from Nephila clavata. Kor. J. Microbiol 37:1-8



Kim, M.G., H.W. Oh, H.M. Park and H.Y. Park (2000) Molecular identification of Wolbachia naturally infected in Thecodiplosis japonensis(Diptera: Cecidominideii) Korean J. Entomol. 30:139-146



Oh H.W., M.G. Kim, S.W. Shin K.S. Bae, Y.J. Ahn and H.Y. Park (2000) Ultrastructural and macular identification of Wolbachia endosymbiont in spider, Nephila clavata. Insect Mol Biol 9:539-543

*******************************

Hyun Woo Oh **^^**

oh0504-at-mail.kribb.re.kr


From daemon Thu Jan 10 23:50:45 2002



From: mhkish :      mhkish-at-cic.aku.ac.ir
Date: Fri, 11 Jan 2002 09:07:10 +0330
Subject: LM. Fiber Identification

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleague;
For identification of fibers we use light microscope efficiently to identify
fibers such as nylon, polyester, cotton, wool, etc. Now there are several
type of fibers in market with one broad and general name. I would like to
know: "Is it possible to identify regular polyester from anti- pill
polyester, using light microscope?
Will you please send me the answer to the following E-mail.
With Regards.
Dr. M. Haghighat Kish, Professor
Synthetic Fiber Research Center
Amirkabir University of Technology
Hafez Ave.
Tehran, Iran
E-Mail: mhkish-at-cic.aku.ac.ir




From daemon Fri Jan 11 05:41:52 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 11 Jan 2002 12:27:19 +0100
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ask Jeol. Jeol Finland has developped a usefull system, the SEM-Aphore, to
digitalise the SEM image in photo quality (3200x4000 pixel). In Europe it
costs somethings like 8000E. It takes wunderfull images. Jeol US should be
able to say you if it works on the T220 A. Collegues have it on a
840.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Fri Jan 11 06:32:45 2002



From: Richard W. Fonda :      fonda-at-anvil.nrl.navy.mil
Date: Fri, 11 Jan 2002 07:26:41 -0500
Subject: Re: sample prep/wire EDM vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I looked into EDMs a couple years ago for similar purposes and was
not able to find any small-sized wire EDMs (for cutting complicated
geometries). Instead we purchased a table-top ram-type EDM from US
EDM Systems (800-837-6808, 7960 S. Roberts Rd., Bridgeview, IL
60455). You can cut TEM disks with a hollow cylinder and can slice
material (up to about 4" wide) with the wire attachment. We've been
generally happy with the versatility and performance of this machine
so far. I was unaware of the Fullam and SBT models at that time, so
I don't know how they compare.

Dick Fonda

_____________________________________________________________
Richard W. Fonda Naval Research Laboratory
(202) 767-2622 Code 6324
(202) 767-2623 fax Washington DC 20375
_____________________________________________________________



At 2:37 PM -0500 1/10/02,
"evgenia.pekarskaya-at-exxonmobil.com"-at-sparc5.microscopy.com wrote:
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From daemon Fri Jan 11 07:39:07 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 11 Jan 2002 08:27:50 -0500
Subject: Re: Digital Camera For JEOL T220 A

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From: Debby Sherman :      dsherman-at-purdue.edu
Date: 11 Jan 2002 08:27:50 -0500
Subject: Re: Digital Camera For JEOL T220 A

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Robert,
Have you tried the Polaroid Type 55 p/n film. Positives do have to be coated but the negatives give 7x the resolution of the positives and are what we are primarily interested in. Digital cameras are great for most SEM work but we still rely on film for situations requiring maximum resolution or when we expect to enlarge the image. Since we deal with primarily low atomic number samples (biological) the problem of "empty magnification" occurs at relatively low magnifications. Take the originals at higher mags is just not equivalent to taking them on film with the option of enlarging the print using a high quality photographic enlarger.

This is not to say that you should not digitize your instrument. We digitized an older instrument a few years ago. I find that most investigators take more pictures than formerly (very good for sampling reasons) and, of course, spend less in the process with less waste. I feel quite strongly that there is definite need for both options and am puzzled that many people buy new instruments without the option of a film camera. Someday digital will be equivalent but I question whether we are there now with the cameras usually supplied with the instruments.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907

On Thursday, January 10, 2002 2:05 PM, robert.fowler-at-tdktca.com {robert.fowler-at-tdktca.com-at-sparc5.microscopy.com} wrote:
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From daemon Fri Jan 11 07:40:24 2002



From: John Foust :      jfoust-at-threedee.com
Date: Fri, 11 Jan 2002 07:24:03 -0600
Subject: Re: Digital Camera For JEOL T220 A

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At 03:20 PM 1/11/2002 +1200, IAN HALLETT wrote:
} For a simple low cost digital image option try looking at ImageSlave
} http://members.ozemail.com.au/~sbwisbey/imageslave.html

ImageSlave and its software look nice. Is there a price?
Also, it seems to use a full-length 8-bit ISA slot, which
is being quite rare these days in new PCs.

As a programmer and tinkerer, I can't help but wonder
what speed and resolution of an analog-to-digital converter
you'd need to watch the slow-scan video output of most SEMs,
and to let the software detect the start and end of scan
lines and image. Is 8-bit enough? Full-speed NTSC color
capture devices are $50-100. You'd think it would be
Simpy A Matter of Programming to tell them to scan less
at slower speeds.

- John



From daemon Fri Jan 11 09:47:14 2002



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Fri, 11 Jan 2002 09:34:05 -0600
Subject: EDS: Human source contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A while ago someone posted a question about reference EDS spectra for human
source contamination (related to semiconductor manufacturing).

I found some references that I'm posting for anyone who's interested:

R.K. Lowry, et al., "Analysis of Human Contaminants Pinpoint Sources of IC
Defects," Semiconductor International, July 1987, p. 73.

R.W. Thomas, "The Identification and Elimination of Human Contamination in
the Manufacture of ICs," Proceedings of the 23rd International Reliability
Physics Symposium, Mar. 26-28, 1985, Orlando, Fla., p. 228.

J.A. Lange, "Sources of Semiconductor Wafer Contamination," Semiconductor
International, April 1983, p. 124.

"Cosmetics, Skin Oils, Flakes Contaminate Clean Rooms," Semiconductor
International, Sept. 1983, p. 20.

The first article contains both EDS and Auger spectra for spittle,
perspiration, sneeze residue and cosmetics residue.

Regards,

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Fri Jan 11 11:07:37 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Fri, 11 Jan 2002 08:55:20 -0800
Subject: Re: sample prep/wire EDM vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Evgenia:

You can find information on our small spark cutter which is designed for
TEM
applications by typing in the key word "TL-SC1" on our website. The
price on the system is $1,895.

If you type in the keyword "EM", you will find an overview of our entire
range of EM related products.

Best regards-

David

"evgenia.pekarskaya-at-exxonmobil.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Dear Colleagues,
}
} I am looking for a wire EDM (spark cutter) for both slicing specimens and
} drilling holes (TEM disks).
} Ideally, I would like to buy a model of a small size (not an industrial
} scale), that could be put on top of a bench.
} I made a search on the Web but it was not very successful.
} I would appreciate if somebody could share his/her experience and recommend
} a vendor, preferably in the US.
}
} Thank you very much.
} Evgenia
}
} **********************************************************
} Evgenia Pekarskaya
} ExxonMobil Research & Engineering Co.
} 1545 Route 22 East, Rm. LB388
} Annandale, NJ, 08801
} Tel. (908) 730-2272
} Fax (908) 730-3355
} e-mail: evgenia.pekarskaya-at-exxonmobil.com

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Fri Jan 11 11:49:23 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 11 Jan 2002 09:44:31 -0800
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



If your goal is not instant results, why not just use
cut sheet 4x5" film? They fit in the same slot that
the Polaroid holder does and cost about 10 cents a
sheet. Of course they have to be processed in a
darkroom but the image quality and tonal range is
superior to Polaroid positives or PN film. Plus, their
exposure latitude is much wider than Polaroid.

gary g.


At 11:05 AM 1/10/2002, you wrote:
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From daemon Fri Jan 11 12:20:39 2002



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Fri, 11 Jan 2002 11:13:40 -0700
Subject: Position--Arizona State University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Research Professional in Electron Microscopy
Center for High Resolution Electron Microscopy
Arizona State University

The Center for Solid State Science seeks applicants for the position of
Assistant/Associate/ Senior Research Professional. This appointment is a
state-funded Academic Professional position on a year-to-year basis.
Essential job functions of this position include: design and engineering of
electronic circuits for analog and digital functions; working closely with
faculty and students to design and manufacture items for research and
development; testing circuit functions to the component level using direct
and indirect trouble-shooting methods for failure diagnosis; and diagnosing
and repairing vacuum and mechanical systems

Required Qualifications: Master's degree in physical or engineering
sciences and five years of experience in electronic repair and maintenance
of analytical equipment; or Bachelor's degree in physical or engineering
sciences and 8 years of experience in electronic repair and maintenance of
analytical equipment. Associate and Full Research Professional ranks also
require a Doctorate degree in related area and/or additional extensive
experience appropriate to rank.

Desired Qualifications:
… Previous experience with electron microscopes
… Experience with low and high power distribution systems
… Demonstrated working knowledge of electron microscopy maintenance and repair

Further information about the Center for High Resolution Electron
Microscopy can be found at www.asu.edu/clas/csss/chrem.

Applicants must submit a cover letter, resume/vitae with names, addresses,
phone numbers and email addresses for three professional references to:
CSSS Research Professional Search Committee, Center for Solid State
Science, PO Box 1704, Arizona State University, Tempe Arizona, 85287-1704.
Application deadline is January 28, 2002, or each Monday thereafter until
position is filled.


Arizona State University is an AA/EO employer




John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Fri Jan 11 13:04:02 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 11 Jan 2002 13:56:03 -0500
Subject: Passive image capture systems

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ian Hallett wrote:
============================================
For a simple low cost digital image option try looking at ImageSlave http:
//members.ozemail.com.au/~sbwisbey/imageslave.html

{ { {snip

} Hello Listers,
} Due to the fact that Polaroid will soon stop production of 667 B&W
instant
} film, I have an immediate need for a digital camera for my JEOL T220 A SEM

From daemon Fri Jan 11 16:22:49 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Fri, 11 Jan 2002 16:15:08 -0600
Subject: EDS System thoughts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like any users to compare and contrast the ease of use and
reliability of the following two EDS systems in a multi-user facility:

EDAX Falcon Genesis with S-UTW/CDU detector or Super-UTW detector

Oxford Inca Energy 200

Please respond offline directly to me.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu



From daemon Fri Jan 11 18:02:14 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 11 Jan 2002 15:57:02 -0800
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ISA is certainly a dead end. Plus, 1Kx1K is not
all that much resolution. If it does the job for some
users, great. I find I mostly capture at 4Kx2600
for 1:5 aspect ratio (same as 35mm slide).

8-bits is plenty of bit depth I think. Regarding speed,
it depends on pixel dwell time. This time affects
image noise and how long you wait for the final image.

Check out these Excel files at http://photoweb.net

mag-ratios.xls
dwelltime.xls

There are no links to them, so load them explicitly.


My ADDA unit will go down to 1.33uS dwell time. I find
that about 7-10uS is optimal for any retained image. Rapid
scan is used for histogram adjustment of contrast and
brightness. Then the slow scan captures the image.
If the image is intended for subsequent deconvolution
focusing, I capture it as 16-bit pixels. This provides the
greatest dynamic range for deconvolution. Otherwise,
8-bits is fine.

So essentially, the A/D conversion time is 1uS or slower.
Not a big deal. Probably the main challenge that could
occur with active scan systems are ground loops. By
having proper isolation and good grounds, synchronizing
to 60Hz will eliminate this problem.

As far as TV rate is concerned, it can be done. But
I don't think it is a direct thing. Direct TV produces
poor resolution. Slow scan with TV readout produces
excellent results. Doing this requires a frame buffer
which collects the scanned info and then outputs it
at TV rate. This output can be easily frame grabbed
at 640x480 8-bit pixels. There are some that are twice
to three times this resolution. But the cost is not low.

gary g.



At 05:24 AM 1/11/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Jan 11 19:19:40 2002



From: Cindy Shannon :      cshannon-at-nctimes.net
Date: Sat, 12 Jan 2002 16:43:12 -0800
Subject: phosphotungstic acid staining of tissue sections for tem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List Server group,
Does anyone use phosphotungstic acid to stain tissue sections for tem?
( or use any other kind of non-radio active staining method )
I would appreciate any information on this subject.
Thank you.
Cindy Shannon
cshannon-at-nctimes.net




From daemon Fri Jan 11 19:41:25 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 11 Jan 2002 19:35:44 -0600
Subject: NSOM Costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A colleague is interested in the purchase price (a range would be
fine) of an NSOM for a wish list they are preparing.

If anyone has good/bad experiences with a particular vendor, this
would be valuable information as well.

I shall forward the info to him. Thank you.

John B.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Sat Jan 12 13:15:29 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 12 Jan 2002 11:07:22 -0800
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Its a passive system. This can be simple or hard, depending
on signal access for a particular SEM. I found that once
spoiled by active scan, passive is passe.

Orion is not the only system with total isolation. The Soft-Imaging
ADDA is fiber optic isolated between PC and SEM electronics.
Perhaps Orion forgot about that?

gary g.


At 10:57 AM 1/12/2002, you wrote:
} Orion has a PCI buss solution - see
} http://www.bright.net/~secmhs/orion_information/orion_digital_imaging_system.htm
}
}
} At 06:57 PM 1/11/02, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Jan 12 13:15:29 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Sat, 12 Jan 2002 13:57:16 -0500
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Orion has a PCI buss solution -
see
http://www.bright.net/~secmhs/orion_information/orion_digital_imaging_system.htm


At 06:57 PM 1/11/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Jan 12 13:15:29 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 12 Jan 2002 11:02:19 -0800
Subject: Re: Digital image capture [take 2]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I'd like to make one clarifying fine point about A/D
conversion of active scan image capture.

Since the purpose of variable pixel dwell time is
to realtime integrate the image captured, the detector
output is not directly converted. I would expect that
the detector output is fed to a sample & hold which
precedes the A/D. The gate to the hold capacitor
is enabled for the pixel dwell time. After this time, the
gate is disabled and a conversion is initiated. Ideally,
the conversion should be as fast as possible--since
either the beam is still at the current position while it
waits for completion of conversion, or the beam moves
to the next pixel while conversion is still underway.

If it is the latter model, then integration is not totally
correct. The beam would be in the new position but
no data was being taken yet. Since this is a slow scan
situation, it seems appropriate to leave the beam as it
until the conversion is completed.

Admittedly a fine point. The folks who have already
implemented these systems certainly would have
already dealt with these issues. Those who are
thinking about new systems or general theory of
image capture may be interested in the finer points.

gary g.



From daemon Sat Jan 12 16:29:27 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Sat, 12 Jan 2002 17:18:53 -0500
Subject: Did Evex get you?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everybody,

I am just curious. Did Evex Spam anybody else directly, using your post
to the listserver, or did they single me out? I was surprised to receive
anything from the Tarquinio brothers, and I made sure I scanned the
attachment for viruses before I looked to see what they were sending
out now.

Take care,
Darrell
Any views expressed are mine, and ABSOLUTELY not my employers.



From daemon Sun Jan 13 17:37:37 2002



From: ctoretta-at-mit.edu ()
Date: Sun, 13 Jan 2002 17:17:46 -0600
Subject: Ask-A-Microscopist: glass bead that is coated with a metal oxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ctoretta-at-mit.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
January 13, 2002 at 14:47:51
---------------------------------------------------------------------------

Email: ctoretta-at-mit.edu
Name: Cara Toretta

Organization: MIT

Education: Undergraduate College

Location: Cambridge, Massachusetts

Question: I am doing research on a 1mm glass bead that is coated with
a metal oxide. I am trying to take digital pictures of the bead to
look for cracks. I am new to microscopy, so i was wondering how it
could be done. I used a normal microscope where the light was emitted
from the bottom, and nothing could really be seen. What is the best
way to get a 30X image and where could i get one of these
microscopes? Thanks.

---------------------------------------------------------------------------


From daemon Sun Jan 13 23:42:27 2002



From: BrigitB22-at-aol.com ()
Date: Mon, 14 Jan 2002 00:33:04 -0500 (EST)
Subject: heya

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(BrigitB22-at-aol.com) on Monday, January 14, 2002 at 00:33:04
---------------------------------------------------------------------------

message: Hi, my name is Brigit and I am a 19 year old female from San Diego, California. Ever since my 14th birthday, I have been really sexually active, but I am still a virgin. Now I am 19 and away from home, attending school at San Diego State University and sharing a dorm with four of my girlfriends and are all VERY turned on to meet a guy and satisfy ALL of his pleasures. To see our sexy pictures we took just last week and to meet some other couples, go to my website {br} { a href="http://www.joinfreee.com"} http://www.joinfreee.com {br} {br} {br} {/a}

---------------------------------------------------------------------------



From daemon Mon Jan 14 02:26:22 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Mon, 14 Jan 2002 09:16:27 +0100
Subject: sample prep/wire EDM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I take avantage of this topic to ask for help. I have an old EDM unit, the
"Servomet Spark Machine" from Metals Research Limited in Cambridge, which
works well in spite of it's say 35 years old. I have a lot of accessories,
the instruction manual, but... a few pages of the manual are lacking, and
of course these with the circuit diagrams.

I have tried to find if Metals Research still exists, with no results. So
does a listmember knows something about that, or perheps has someone the
same machine, and could send me these diagrams.

Thanks to all

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Mon Jan 14 06:56:19 2002



From: Isabel Nogueira :      isabeln-at-popsrv.ist.utl.pt
Date: Mon, 14 Jan 2002 12:48:12 -0000
Subject: Problems with dimpler model D500i (VCR, now South Bay)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have a VCR dimpler Model D500i which is not working. There seems to be an
electronic problem, but so far we haven't been able to isolate the cause.
Our main difficulty is that the manual has no diagrams of the circuits and
that makes it twice as difficult to locate the origin of the problem.
We have contacted South Bay Technology, who have acquired VCR, asking for
such diagrams and the answer we got was "send us the equipment".
I would like to know if anyone out there as experienced this kind of
problems and/or if anyone knows where we could get the electronic diagrams
of such a dimpler.

Thank you in advance,

Isabel



Isabel Nogueira
Instituto Superior Técnico
Dep. Materiais
Avenida Rovisco Pais
1049-001 Lisboa
Portugal
tel.: +351 218418123
fax: +351 218418120
email: isabeln-at-popsrv.ist.utl.pt




From daemon Mon Jan 14 08:17:25 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Mon, 14 Jan 2002 08:00:59 -0600
Subject: Wrinkles in sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Darrell and Listers,

I see you received an unsolicited e-mail. (Less then 20 emails sent total)
OOPS! my apologies.
Please disregard that email. Please rest assured the email was an adobe
acrobat file, and anti-virus is installed on all machines.


Regards
Peter
Evex Analytical








Evex Analytical
Microanalysis and Digital Imaging
857 State Road
Princeton, NJ 08540
609-252-9192 T
609-252-9091 F
www.evex.com
sales-at-evex.com
----- Original Message -----
} From: "Darrell Miles" {milesd-at-US.ibm.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, January 12, 2002 5:18 PM


Listies

I have wrinkles in my sections that are already on grids. The sections
didn't appear to have wrinkles when I collected them.

Will chloroform work on wrinkles after the sections are on the grids?

Is there another method?

Tim Quinn
University of Kansas
Research Assistant
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu


From daemon Mon Jan 14 09:15:16 2002



From: Sara_Lundgren-at-s-and-s.com
Date: Mon, 14 Jan 2002 10:05:28 -0500
Subject: Please unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Unsubscribe




From daemon Mon Jan 14 09:25:55 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Mon, 14 Jan 2002 07:42:28 -0800 (PST)
Subject: Re: phosphotungstic acid staining of tissue sections for tem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Oh, I see. You 'accidentally' used one of my posts to send an "unsolicited
e-mail."
Spam is spam, no matter how you want to paint it, or how few your
experiment
reached.

Darrell



"Evex" {ptarq-at-ns1.tradezone.net} on 01/14/2002 09:08:23 AM

Please respond to "Evex" {ptarq-at-ns1.tradezone.net}

To: {Microscopy-at-sparc5.microscopy.com} , Darrell Miles/Fishkill/IBM-at-IBMUS
cc:



Hi Cindy,
We use PTA on thin sections for TEM to stain the collagen. We use a 1%
solution in distilled water. Leave it as an acidic pH and filter at least
twice through a #1 filter. Best to use it fresh. 30 min staining time done
before UA and lead.

Bob
Derm Research Center
U of W
Seattle

On Sat, 12 Jan 2002, Cindy Shannon wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear List Server group,
} Does anyone use phosphotungstic acid to stain tissue sections for tem?
} ( or use any other kind of non-radio active staining method )
} I would appreciate any information on this subject.
} Thank you.
} Cindy Shannon
} cshannon-at-nctimes.net
}
}
}
}



From daemon Mon Jan 14 10:12:57 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 14 Jan 2002 11:25:18 -0500
Subject: Core Facility Management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Before this gets out of hand, I would like to ask that we drop this subject right now.

This is bordering on the personal side. There was an apology given.

Let's remember that Nestor moderates this forum very effectively and fairly.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Darrell Miles [mailto:milesd-at-US.ibm.com]
Sent: Monday, January 14, 2002 10:18 AM
To: Microscopy-at-sparc5.microscopy.com



Oh, I see. You 'accidentally' used one of my posts to send an "unsolicited
e-mail."
Spam is spam, no matter how you want to paint it, or how few your
experiment
reached.

Darrell



"Evex" {ptarq-at-ns1.tradezone.net} on 01/14/2002 09:08:23 AM

Please respond to "Evex" {ptarq-at-ns1.tradezone.net}

To: {Microscopy-at-sparc5.microscopy.com} , Darrell Miles/Fishkill/IBM-at-IBMUS
cc:

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


List members,
I am in the process of organizing the session on Core Facility Management for M&M 2002. I would appreciate receiving contact information for the following:

1) names of staff members at your school/company who serve as on-site service engineers. These are individuals who maintain electron microscopes and related equipment that are not on OEM service contracts.

2) Individuals at academic institutions who are actively involved in doing microscopy for commercial companies.

3) Individuals from Private for-profit companies who do microscopy for commercial companies or academic institutions.

Thanks in advance,
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907




From daemon Mon Jan 14 14:30:15 2002



From: Mary McKee :      mckee-at-helix.mgh.harvard.edu
Date: Mon, 14 Jan 2002 14:18:39 -0600
Subject: digital imaging for Philips CM10 TEM

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Message-Id: {p05100300b868ed9e4866-at-[206.69.208.21]}


Dear listmembers,

We're getting to the point of seriously considering adding a digital camera
and workstation to our Philips CM10 TEM. I was wondering if anyone has done
a retrofit, what equipment worked well for them, etc. Thanks.

Mary

Mary McKee
MGH Renal Unit
Charlestown, MA 02129
(617)726-3696


From daemon Mon Jan 14 15:53:52 2002



From: Bob :      bobrobs-at-earthlink.net
Date: Mon, 14 Jan 2002 14:46:16 -0700
Subject: Re: digital imaging for Philips CM10 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Mary,

I would recommend taking a close look at Emispec Systems, Inc. Their
system offers the capability for future
expansion that includes all detectors associated with TEM, should your
needs from just digital imaging.

Bob Roberts
EM Lab Services, Inc.
2409 S. Rural Rd Suite C
Tempe, Arizona 85282
480.967.3946


Mary McKee wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear listmembers,
}
} We're getting to the point of seriously considering adding a digital
} camera
} and workstation to our Philips CM10 TEM. I was wondering if anyone
} has done
} a retrofit, what equipment worked well for them, etc. Thanks.
}
} Mary
}
} Mary McKee
} MGH Renal Unit
} Charlestown, MA 02129
} (617)726-3696
}
}




From daemon Mon Jan 14 17:44:41 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 14 Jan 2002 16:34:12 -0700
Subject: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robert,

you are not the only one in this situation. We have had a number of people
who called us and asked the same question. So perhaps a bit of general
information helps everybody.

Replacing your Polaroid unit with a digital camera may be doable, but is
definitely not straightforward. your Polaroid unit consists of a high
resolution CRT and a Polaroid camera. It may be possible to take out the
camera and put in a digital camera instead, but there are certain things you
need to keep in mind and they may well create problems that can't be
overcome. First, you would have to find a lens, which would allow you to
image the monitor without distortions. You could probably use a macro lens,
but depending on the distance from the monitor and the lens, you could
potential introduce distortions. Second, you need to be able to use an
exposure time of however long it takes to take a photo (2 minutes?). That
may not be possible, depending on software and camera. You may also need a
cooled camera to reduce the amount of dark current accumulated during this
time. Third, the camera is digital with discrete pixels in X and Y. The
phototube is analog in X, but discrete in Y direction. If you are not
careful, you can create Moire Effects from mismatch between the number of
lines and the Y-resolution of the camera. Fourth, I am not so sure, what
kind of non-linearities in the signals are created by the
detector-amplifier-CRT-camera chain.

This leaves of course the direct acquisition of the image signals. If your
SEM has a TV output, you can certainly use a relatively cheap image
acquisition card. However, you would have to run your SEM in TV mode to use
this. The problem is, that TV is pretty much limited to 640x480x8 (NTSC) or
756x564x8 (PAL), i.e., low resolution. If you purchase one of those cards,
stay away from ISA cards. That's technology that went out of fashion with
the Pentium computers, and computers with ISA slots are hard to find these
days. Even worse is EISA, which was never very popular to begin with. The
current technology uses PCI slots, with many cards probably moving to
FireWire (IEEE 1394) in the near future. You also want to make sure you can
frame average to reduce noise.
Unfortunately, it is not so easy to digitize the signals from non-standard
signals. If you have NTSC or PAL, which are TV standards, it is very simple
to acquire and decode the signals, simply because they are standards, and
precisely defined. However, the slower scan (non-standard) signals require,
that one can change timings and/or levels and delays to identify line
retrace and screen retrace signals. This makes them much more expensive.
There is simply no mass production for these devices.

I will not further comment on Gary Gaugler's excellent remarks about passive
and active systems and optical data transmission (suffice it to say that we
were to my knowledge the first ones to implement it).

To summarize: There are various options to replace a Polaroid unit on an
SEM. The price range of 2-3K is a bit "optimistic", as these devices cannot
be "mass-produced" like frame grabbers, around 10K is a more realistic
price. On the other hand, you save about $2-$3 per photo, and do not create
a lot of chemical waste in the process. I'd be more than happy to discuss
this in more detail with you, but we should probably not do that on the list
server.

Mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com
[mailto:"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com]
Sent: Thursday, January 10, 2002 12:06 PM
To: Microscopy-at-sparc5.microscopy.com


Hello Listers,
Due to the fact that Polaroid will soon stop production of 667 B&W instant
film, I have an immediate need for a digital camera for my JEOL T220 A SEM.
Any help (vendors welcome) would be appreciated on or off listserver.
Budget is around 2 - 3k. I am down to my last few boxes, and although I
still can reorder I prefer not to................

TIA

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



From daemon Mon Jan 14 17:56:40 2002



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Mon, 14 Jan 2002 18:52:34 -0500
Subject: RE: A non-radio active positive stain for sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cindy:
Phosphotungstic acid has indeed been used, but as a substitute for uranyl
acetate stain, I much prefer bismuth for staining sections on grids.
Bismuth tends to act as a general contrast enhancing stain for sections due
in part to its ability to interact with reduced osmium. It stains DNA,
ferritin, glycogen, lysosomes, polysaccharides, and ribosomes. The one
caveat is that at high mags bismuth stain graininess becomes apparent.
Bismuth can be used with uranyl and lead stain to achieve an even greater
contrast enhancing effect.
Hope this helps,
Henry

Cindy Shannon wrote:.............................
"Dear List Server group,
Does anyone use phosphotungstic acid to stain tissue sections for tem?
( or use any other kind of non-radio active staining method )
I would appreciate any information on this subject.
Thank you.
Cindy Shannon
cshannon-at-nctimes.net"





From daemon Mon Jan 14 19:34:14 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Mon, 14 Jan 2002 19:26:18 -0600
Subject: Low Vac SEM

Contents Retrieved from Microscopy Listserver Archives
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Group

Looking for anyone who is using Low Vacuum (poor vacuum) SEM to image and
characterize insulating polymer materials. I am not very familiar with this
type of system but understand the basic idea of how it works. Want to
explore the practical side of looking at non-coated insulators or water
containing samples from both an imaging and EDS analysis perspective.

Prefer direct replies.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu


From daemon Mon Jan 14 20:10:32 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Mon, 14 Jan 2002 21:12:59 -0500
Subject: Re: Ask-A-Microscopist: glass bead that is coated with a metal oxide

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Dear Cara,

Your problem is a good example of the general problem of defining
inspection criteria, since the lighting may critically influence what
one can see. I once had a similar problem looking for cracks in a
graphite coating on quartz fibers. The answerto your question depends a
bit on the size of the cracks and the opacity and reflectivityof the
oxide coated surface. But you should ensure that you have a lighting set
up that allows you to dependably see the cracks before worrying about
the camera. Most of the digital cameras that can be used with a
microscope would probably be adequate to recording the crack, once you
get the viewing situation set up.

Low magnification approaches include the following:
You might first try the microscope that you used before with lighting as
follows: If it has a condenser lens below the sample position,
experiment with placing a sheet of something opaque with a small hole in
it in the light path so that the light is blocked from passing around
your sample. Then you will have a better chance to see any light passing
through your glass bead due to a crack in the coating. You might want to
turn off the room lights to get the best view.

Alternatively, you could arrange for "dark field" illumination by doing
the opposite: cut some disks (or try some coins in different sizes) and
hold them with tweezers in the lightpath beneath the condenser lens so
that light cannot pass through the sample bead directly but must bounce
off the sides of the sample to be seen. This may be effective if the
bare glass in the cracks is more reflective than the coating.

A 1mm round object could be observed well under a stereo-binocular
microscope common in many biology labs as a "dissecting microscope".
This has the advantage of an upright image that will make turning the
sphere to view it from all sides easier, and it has good "depth of
field". However, you would need to arrange the lighting to suit your
problem and if the cracks are very narrow, you might have to inspect at
a magnification that exceeds the upper limit of such a 'scope. The light
generally comes from above on such a microscope. Some microscopes of
this kind are equipped with a "ring light", a small fluorescent lamp or
a fiber optic fixture around the lens that gives a very diffuse light.
This might be helpful if both the bead and the coating are shiny and
tend to reflect strongly.

Another low mag ( or no mag!) approach might be possible with a fiber
optic lamp if one is available and your coating is opaque. If you make
an opaque cover to fit over the end of the fiber optic with a hole in it
that is smaller than 1mm, you might be able to see light projected
through a crack when you place the bead over the hole. Again, use a
darkened room to see the light projected through the bead. If this
works, orient the fiber optic and bead so that they can be observed with
the microscope while you're doing this.

For very narrow cracks there may be no alternative to using
magnification that is too high to allow you to see the entire bead in
focus at one time. In that case, there may be no alternative to racking
the focus up and down and systematically rotating the bead to all
orientations. If you can find a metallurgical microscope (engineering,
materials science departments) it will have a simple means of switching
between bright field and dark field reflected light. Either could be
best, depending on the reflectivities involved.

John Twilley
Conservation Scientist



ctoretta-at-mit.edu {mailto:ctoretta-at-mit.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (ctoretta-at-mit.edu {mailto:ctoretta-at-mit.edu} ) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
} January 13, 2002 at 14:47:51
} ---------------------------------------------------------------------------
}
}
} Email: ctoretta-at-mit.edu {mailto:ctoretta-at-mit.edu}
} Name: Cara Toretta
}
} Organization: MIT
}
} Education: Undergraduate College
}
} Location: Cambridge, Massachusetts
}
} Question: I am doing research on a 1mm glass bead that is coated with
} a metal oxide. I am trying to take digital pictures of the bead to
} look for cracks. I am new to microscopy, so i was wondering how it
} could be done. I used a normal microscope where the light was emitted
} from the bottom, and nothing could really be seen. What is the best
} way to get a 30X image and where could i get one of these microscopes?
} Thanks.
}
} ---------------------------------------------------------------------------
}
}
}
}




From daemon Mon Jan 14 20:25:08 2002



From: JLCastner-at-aol.com
Date: Mon, 14 Jan 2002 21:19:37 EST
Subject: Brightfield Photomicroscopy w Nikon D1X Camera

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

I am a scientific photographer, but have done little in the way of
photomicroscopy. I am in the process of evaluating compound microscopes for
purchase to use specifically with a Nikon D1X professional digital camera. I
will be taking brightfield photomicrographs of prepared botanical specimens
(such as root cross sections, anther cross sections, etc.). I would like to
speak to anyone who has used a Nikon D series digital camera on a compound
microscope in similar photomicroscopy applications. Perhaps you can help me
avoid making an expensive purchasing error.

Thank you very much, and please feel free to reply privately if you feel
most of the list would not be interested.

Sincerely,

Jim Castner
352-371-6439
jlcastner-at-aol.com


From daemon Mon Jan 14 21:59:31 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Mon, 14 Jan 2002 22:58:55 -0500
Subject: Re: Digital Camera For JEOL T220 A

Contents Retrieved from Microscopy Listserver Archives
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Debbie,
In general, I fully agree with you, especially for those still using
ETECs because the recording system can actually resolve all 2000 lines
in a scan. However, I was recently at JEOL headquarters in Danvers, MA
and saw sometruly outstanding digital images in their lobby. They are
several feet squareand digitally scanned at 16k x 16k (at least one was
made up of 4 of these).

File size still presents a major problemfor digital as you could only
fit 2 of these on a CD-R (256M each or 2 CD-Rs for the 4 part montage).
However, 2k x 2k (4M file) is much more reasonable than it was just a
couple of years ago in terms of both storage costs and processing time
and 4k x 4k, I feel, is probably as good as you'll see off a CRT at 2000
lines. Of course if you're just printing on plain paper, you'll never
get an equivalent image, but if you use "photo" paper in your inkjet,
you're up to a buck a shot, and still not quite as good as film. For
those who ponder this quandry, try glossy brochure paper. It's a lot
cheaper and works almost as well as the "photo" paper.

It pains me to see the lack of interest in high quality photographs in
all areas, but the digital is rapidly getting better, as are the inkjet
printers.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA


Debby Sherman wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Robert,
} Have you tried the Polaroid Type 55 p/n film. Positives do have to be coated but the negatives give 7x the resolution of the positives and are what we are primarily interested in. Digital cameras are great for most SEM work but we still rely on film for situations requiring maximum resolution or when we expect to enlarge the image. Since we deal with primarily low atomic number samples (biological) the problem of "empty magnification" occurs at relatively low magnifications. Take the originals at higher mags is just not equivalent to taking them on film with the option of enlarging the print using a high quality photographic enlarger.
}
} This is not to say that you should not digitize your instrument. We digitized an older instrument a few years ago. I find that most investigators take more pictures than formerly (very good for sampling reasons) and, of course, spend less in the process with less waste. I feel quite strongly that there is definite need for both options and am puzzled that many people buy new instruments without the option of a film camera. Someday digital will be equivalent but I question whether we are there now with the cameras usually supplied with the instruments.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu {mailto:dsherman-at-purdue.edu}
} S-052 Whistler Building
} West Lafayette, IN 47907
}
} On Thursday, January 10, 2002 2:05 PM, robert.fowler-at-tdktca.com {mailto:robert.fowler-at-tdktca.com} {robert.fowler-at-tdktca.com-at-sparc5.microscopy.com} {mailto:robert.fowler-at-tdktca.com-at-sparc5.microscopy.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello Listers,
} } Due to the fact that Polaroid will soon stop production of 667 B&W instant
} } film, I have an immediate need for a digital camera for my JEOL T220 A SEM.
} } Any help (vendors welcome) would be appreciated on or off listserver.
} } Budget is around 2 - 3k. I am down to my last few boxes, and although I
} } still can reorder I prefer not to................
} }
} } TIA
} }
} } Robert Fowler
} } Quality Assurance Technician (Failure Analysis)
} } TDK Components USA, Inc.
} } Multilayer Ceramic Capacitor Division
} } 1 TDK Boulevard
} } Peachtree City GA 30269-2051
} } Telephone: (770) 631-0410 Ext.315
} } Fax: (770) 487-1460
} } email: rfowler-at-tdktca.com {mailto:rfowler-at-tdktca.com}
} } www.tdk.com {http://www.tdk.com}
} }
} }




From daemon Tue Jan 15 10:59:28 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 15 Jan 2002 16:44:49 +0000 (GMT Standard Time)
Subject: Re: RE: A non-radio active positive stain for sections

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I would be grateful if you would post your bismuth staining
protocol.

Dave


On Mon, 14 Jan 2002 18:52:34 -0500 Henry Eichelberger
{heichelb-at-binghamton.edu} wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Cindy:
} Phosphotungstic acid has indeed been used, but as a substitute for uranyl
} acetate stain, I much prefer bismuth for staining sections on grids.
} Bismuth tends to act as a general contrast enhancing stain for sections due
} in part to its ability to interact with reduced osmium. It stains DNA,
} ferritin, glycogen, lysosomes, polysaccharides, and ribosomes. The one
} caveat is that at high mags bismuth stain graininess becomes apparent.
} Bismuth can be used with uranyl and lead stain to achieve an even greater
} contrast enhancing effect.
} Hope this helps,
} Henry
}
} Cindy Shannon wrote:.............................
} "Dear List Server group,
} Does anyone use phosphotungstic acid to stain tissue sections for tem?
} ( or use any other kind of non-radio active staining method )
} I would appreciate any information on this subject.
} Thank you.
} Cindy Shannon
} cshannon-at-nctimes.net"
}
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Jan 15 14:22:18 2002



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Tue, 15 Jan 2002 15:16:21 -0500
Subject: Contact info for Scanning Microscopy International

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Hello Microscopists:

I'd like to obtain permission to reproduce a figure from a paper in
Scanning Microscopy Supplement, Volume 10 (1996), a.k.a the Proceedings of
the 14th Pfefferkorn Conference. The publisher is listed as Scanning
Microscopy International, with the following contact information:

Dr. Om Johari
P.O. Box 66507
Chicago (A.M.F. O'Hare), IL 60666-0507
USA

E-mail: 73211.647-at-compuserve.com
Web: http://hem2.passagen.se/smi/
Tel: 847-524-6677

But... the phone number has been disconnected, there's no reply to mail or
e-mail, and the web address gives a "page not found" default (in Swedish);
searching this ISP gave me no results, and neither do the regular search
engines (Google, etc.).

Does anyone know what happened to Scanning Microscopy International and how
to contact them, or who owns the copyright to this publication now?

Thanks in advance,

Rick Powell


*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Tue Jan 15 17:59:04 2002



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Tue, 15 Jan 2002 17:49:04 -0600 (CST)
Subject: Surface session at Durban ICEM

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As I am sure you are already aware, the deadline for abstracts for
the 15th International Conference on Electron Microscopy, Durban,
South Africa (http://www.icem15.com) is Febuary 1st - rapidly
approaching. I am organizing a session on "Instrumentation and
Characterization of Surfaces". Loosely I would describe the session
as covering:

a) All TEM/SEM/STEM based techniques for obtaining surface
sensitive signals from surfaces, particularly under controlled
conditions (e.g. UHV, controlled gas).

b) All TEM/SEM/STEM techniques for obtaining nanoscale or
atomic scale information from surfaces. This would include both
profile and plan view imaging of surfaces.

c) Any new types of instrumentation for obtaining surface
information.

d) Other types of surface microscopies, for instance HREM
in plan or profile modes, LEEM, PEEM, REM.

e) New methods of obtaining atomic-scale surface information,
e.g. Direct Methods.

f) Applications of surface-sensitive techniques to problems,
e.g. heterogeneous catalysis, semiconductor or oxide growth.

I hope you will have the opportunity to submit an abstract. Please
feel free to contact me if you have any questions.

-----------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
-----------------------------------------------




From daemon Wed Jan 16 01:57:46 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Wed, 16 Jan 2002 08:50:10 +0100
Subject: Fw: Brightfield Photomicroscopy w Nikon D1X Camera

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Hi Jim,

Here at our departement I've been using the Nikon D1 on a Zeiss Stemi 2000 C
and Sv 11 to take photo's of whole mice and organs.
I've to say that with 800 ISO I still needed a shutter time of about more
than 1/10 to get a bright image, but you can fix the camera, so that's not a
real problem in general, only when taking images of live mice it's not
always easy. The Stemi is a stereomicroscope , I don't know if it's such a
one you would like to use?!
The only "problem", don't call it a real problem, but you know what I mean,
is to set up the correct white balance. When you make a small change in the
light intensity of the microscope, it can result in a big change for the
white balance. But in general, I, and the professors/students, are very
satisfied with the results. I hope I was of some help for you, if you need
some more info, feel free to ask!
Best regards,

Sven

__________________________________________
Sven Terclavers
Research Assistent
Center for Molecular and Vascular Biology
Center for Transgene Technology and Gene Therapy
Campus Gasthuisberg O/N
Herestraat 49
3000 Leuven
Belgium
___________________________________________


----- Original Message -----
} From: {"JLCastner-at-aol.com"-at-sparc5.microscopy.com}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, January 15, 2002 3:19 AM
} Subject: Brightfield Photomicroscopy w Nikon D1X Camera
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello All,
} }
} } I am a scientific photographer, but have done little in the way of
} } photomicroscopy. I am in the process of evaluating compound microscopes
} for
} } purchase to use specifically with a Nikon D1X professional digital
camera.
} I
} } will be taking brightfield photomicrographs of prepared botanical
} specimens
} } (such as root cross sections, anther cross sections, etc.). I would
like
} to
} } speak to anyone who has used a Nikon D series digital camera on a
compound
} } microscope in similar photomicroscopy applications. Perhaps you can
help
} me
} } avoid making an expensive purchasing error.
} }
} } Thank you very much, and please feel free to reply privately if you
} feel
} } most of the list would not be interested.
} }
} } Sincerely,
} }
} } Jim Castner
} } 352-371-6439
} } jlcastner-at-aol.com
} }
} }
}



From daemon Wed Jan 16 07:10:02 2002



From: a7528922 :      a7528922-at-cic.aku.ac.ir
Date: Wed, 16 Jan 2002 06:59:37 -0600
Subject: SEM. polaroid film type 52

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Dear Colleagues;
We have a HITACHI SEM. We use Polaroid Type 52 for making pictures. Is
there any
replacement for this type of film without replacing the holder on the
microscope? Where one can buy this type of film? Type 52 seems to be very
rare and it can not be found in the
market.
M. H. Kish
mhkish-at-cic.aku.ac.ir


From daemon Wed Jan 16 07:22:34 2002



From: Val Moshkovskiy :      Moshkovskiy-at-rochester.mellesgriot.com
Date: Wed, 16 Jan 2002 08:21:26 -0500
Subject: FW: Need a Study

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Can anyone recommend a market research firm or consultant to conduct a
} study on an optical instrument to be used in microscopy. Please, respond
} to moshkovskiy-at-rochester.mellesgriot.com
}
} Thank you,
}
} Val Moshkovskiy
}


From daemon Wed Jan 16 08:06:28 2002



From: Tom :      Hanley_Tom-at-colstate.edu
Date: Wed, 16 Jan 2002 09:00:11 -0400
Subject: high power objective for Leitz pol

Contents Retrieved from Microscopy Listserver Archives
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I would like to buy a 60 or 100 X objective for one of our Leitz
Laborlux 11 pol. I have seen a couple on ebay but am reluctant. I
am not sure they would fit. Is there a more dependable source?

The documentation that came with our Leitz scopes has drifted away.

Thanks.
-------------------------------------------------------------------
Dr. Tom Hanley, Department of Chemistry and Geology, Columbus State U.,
4225 University Ave., Columbus, Georgia 31907-5645.
Links to the ACRES project and to 1998 and 1999 pictures I took in Panama may be found at:
http://chemgeo.ColState.edu/th_hp.htm
VOX: 706-568-2075; FAX: 706-569-3133.


From daemon Wed Jan 16 09:10:33 2002



From: Pierre-M. Charest :      pcharest-at-rsvs.ulaval.ca
Date: Wed, 16 Jan 2002 10:00:23 -0500
Subject: Meeting - Microscopy & Microanalysis 2002

Contents Retrieved from Microscopy Listserver Archives
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Quebec City will hot the Microscopy & Microanalysis 2002 Meeting on
August 4-8. Deadline for abstract submission is February 15. All
information concerning the scientific program and the abstract
submission process is available on the MSA web site at the following
URL address:

http://www.microscopy.com/MSAMeetings/MMMeeting.html


Quebec City is an exciting destination also for you vacation. The
local French culture is charming and people are very open to
visitors. The historic Old City as well as the close wild nature of
the Laurentides are a Guarantee of the success of your next summer
vacation. For a foretaste of Quebec City, please check the Local
Arrangement Committee web site at:

http://msc.rsvs.ulaval.ca


Pierre M. Charest, Chair
LAC - M&M 2002
pcharest-at-rsvs.ulaval.ca
--
******************************************
MICROSCOPY& MICROANALYSIS 2002
QUEBEC CITY 4-8 AUGUST 2002
VISIT OUR WEB SITE AT:
http://msc.rsvs.ulaval.ca/2002/2002.html
http://www.microscopy.com/MSAMeetings/MMMeeting.html
******************************************
Dr. Pierre M. Charest, Professeur titulaire
Departement de phytologie
Pavillon C.-E. Marchand, bureau 4245
Universite Laval
Sainte-Foy, Que., CANADA
G1K 7P4
Tél. (418) 656-7792 (bur), 656-2131 #6629 (lab)
Fax (418) 656-7176
Email: pcharest-at-rsvs.ulaval.ca
http://msc.rsvs.ulaval.ca
http://www.rsvs.ulaval.ca/
http://alpha.eru.ulaval.ca/phytowww/CV/pm_charest.html
http://www.crefsip.ulaval.ca/
******************************************


From daemon Wed Jan 16 09:25:41 2002



From: Robin Scribailo :      RScrib-at-purduenc.edu
Date: Wed, 16 Jan 2002 08:41:25 -0600
Subject: Dear Microscopy listserver,

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopy listserver,

I have a Nikon SMZ-U and Nikon optiphot 2 microscope that I am trying to adapt to digital photography. Both have trinocular tubes and are hooked up to a Nikon HFX-DX 35 mm camera system. I was informed that the Nikon coolpix 5000 cannot be adapted to these microscopes because Nikon does not (and does not plan to) make a lens for this. As a result I purchased their coolpix MDC lens and a Diagnostic instruments, Inc. Part # D 10 NLC C-Mount for a 38 mm ISO port to adapt for the Nikon Coolpix 995 .
I have since learned that a stop-down ring is made for the coolpix 5000 to adapt it to optional lenses for the coolpix 995. Would this work with the microscopes or would parfocality be a problem?

I have been told that the megapixel difference between the two cameras will not make any difference to image quality particularly at higher mag since the microsocope aperture size will limit this anyway.

Part of the issue here is that the camera will also be used for outdoor photography and close-ups. Although the megapixels may not make a difference for microscopy it will make a difference for outdoor shots where the images will be blown up considerably.

Any advice would be most appreciated. Please send the information directly to me and I will summarize for the group.

Sincerely,

Robin

Robin W. Scribailo Ph.D.
Associate Professor of Biological Sciences
Director of the Aquatic Plant Herbarium
Biological Sciences
Purdue University North Central
1401 S. U.S. 421
Westville, IN 46391-9528
(219) 785-5255
Fax (219) 785-5483




From daemon Wed Jan 16 09:37:25 2002



From: Kevin Mackenzie :      k.s.mackenzie-at-abdn.ac.uk
Date: Wed, 16 Jan 2002 15:29:45 +0000
Subject: TEM collagen fibrils

Contents Retrieved from Microscopy Listserver Archives
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A colleague is planning to investigate collagen reinforcement in natural
tissues using the TEM.
Part of the project is to measure the diameter and spacing of the collagen
fibrils. However they are finding that the preparation of the tissue is
changing both the diameters and spacing of the fibres.

Has anyone suggestions/comments on how to improve tissue structure or any
other alternative methods.


thanks


Kevin
Electron Microscope unit
Department of Zoology
University of Aberdeen
Aberdeen
AB24 2TZ

Tel 01224-272847
Fax 01224-272396
------------
Kevin Mackenzie
k.s.mackenzie-at-abdn.ac.uk



From daemon Wed Jan 16 09:46:30 2002



From: Mark Germani :      mgermani-at-micromaterialsresearch.com
Date: Wed, 16 Jan 2002 09:38:35 -0600
Subject: Electron Microscopy and Microanalysis Short Course

Contents Retrieved from Microscopy Listserver Archives
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Electron Microscopy and Microanalysis is a one-day short course to be held
on Sunday March 17, 2002 at PITTCON 2002 in New Orleans. The course is an
introduction to SEM, TEM and x-ray microanalysis and is designed for
analytical chemists and others who need to use these techniques for
industrial problem-solving and product research and development. For more
information and online registration visit www.pittcon.org. The short course
is number 229 and can be found under the Microscopy section on the Short
Courses web page.

Mark Germani, Ph.D.
MicroMaterials Research, Inc.
136 Shore Drive, #200
Burr Ridge, IL 60521

(630) 325-8170
(630) 325-8178 fax

mgermani-at-micromaterialsresearch.com





From daemon Wed Jan 16 11:56:26 2002



From: Tina Schwach :      tschwach-at-mindspring.com
Date: Wed, 16 Jan 2002 11:47:22 -0600
Subject: Contact info for Scanning Microscopy International

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick,
The organization is Scanning and you can find them at www.scanning-fams.org.

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112
----- Original Message -----
} From: Rick Powell at Nanoprobes
To: microscopy-at-sparc5.microscopy.com
Sent: Tuesday, January 15, 2002 2:16 PM


Hello Microscopists:

I'd like to obtain permission to reproduce a figure from a paper in
Scanning Microscopy Supplement, Volume 10 (1996), a.k.a the Proceedings of
the 14th Pfefferkorn Conference. The publisher is listed as Scanning
Microscopy International, with the following contact information:

Dr. Om Johari
P.O. Box 66507
Chicago (A.M.F. O'Hare), IL 60666-0507
USA

E-mail: 73211.647-at-compuserve.com
Web: http://hem2.passagen.se/smi/
Tel: 847-524-6677

But... the phone number has been disconnected, there's no reply to mail or
e-mail, and the web address gives a "page not found" default (in Swedish);
searching this ISP gave me no results, and neither do the regular search
engines (Google, etc.).

Does anyone know what happened to Scanning Microscopy International and how
to contact them, or who owns the copyright to this publication now?

Thanks in advance,

Rick Powell


****************************************************************************
*************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
****************************************************************************
*************



From daemon Wed Jan 16 12:12:31 2002



From: Ken Gaugler :      ken-at-gaugler.com
Date: Wed, 16 Jan 2002 10:10:07 -0800
Subject: Re: Service for ISI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sandra,

Richard Murphy is an excellent service engineer and who worked for ISI
for
many years. He gave me great support when I was at Lawrence Berkeley
Laboratory and elsewhere. I will forward his pager number to you.

Best Regards,
Ken Gaugler

S Keller wrote:

}
}
} Hi All:
} There was a thread about ISI service. Who
} is now responsible for their service?
} Thanks in advance,
} Sandra
}
}

--
Ken Gaugler
Santa Clara, CA.
~~ spark: N6OSK ~~
PGP: 2EF7 437E 1D0B 602D BC2C 92D1 A548 C11B B901 8CDE





From daemon Wed Jan 16 16:27:03 2002



From: Don Grimes :      microtoday-at-mindspring.com
Date: Wed, 16 Jan 2002 16:58:40 -0600
Subject: Contact info for Scanning Microscopy International

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tina,
I humbly suggest that you are making a mistake. What this guy is looking for
is what us old folks called the "SEM Conference" - which Om Johari ran for
many years and which he closed down a number of years ago.
"Scanning", a meeting and a publication, is a more recent operation run by
the FAMS group and which is still in existance. I do not believe, and I
could be wrong, that the new "SCANNING" acquired the old "SEM Conference".
The last address that I had for Om has been given to the requester.
Regards,
Don Grimes, Microscopy Today

-----Original Message-----
} From: Tina Schwach [mailto:tschwach-at-mindspring.com]
Sent: Wednesday, January 16, 2002 11:47 AM
To: microscopy-at-sparc5.microscopy.com; Rick Powell at Nanoprobes


Rick,
The organization is Scanning and you can find them at www.scanning-fams.org.

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112
----- Original Message -----
} From: Rick Powell at Nanoprobes
To: microscopy-at-sparc5.microscopy.com
Sent: Tuesday, January 15, 2002 2:16 PM


Hello Microscopists:

I'd like to obtain permission to reproduce a figure from a paper in
Scanning Microscopy Supplement, Volume 10 (1996), a.k.a the Proceedings of
the 14th Pfefferkorn Conference. The publisher is listed as Scanning
Microscopy International, with the following contact information:

Dr. Om Johari
P.O. Box 66507
Chicago (A.M.F. O'Hare), IL 60666-0507
USA

E-mail: 73211.647-at-compuserve.com
Web: http://hem2.passagen.se/smi/
Tel: 847-524-6677

But... the phone number has been disconnected, there's no reply to mail or
e-mail, and the web address gives a "page not found" default (in Swedish);
searching this ISP gave me no results, and neither do the regular search
engines (Google, etc.).

Does anyone know what happened to Scanning Microscopy International and how
to contact them, or who owns the copyright to this publication now?

Thanks in advance,

Rick Powell


****************************************************************************
*************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
****************************************************************************
*************





From daemon Wed Jan 16 16:42:20 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 16 Jan 2002 16:42:29 -0600
Subject: Texas Society for Microscopy Sring Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All: the Texas Society for Microscopy Spring meeting will
be held in Fort Worth, TX
on April 18-20 at the Ramada Plaza Hotel, 1701 Commerce St.,
Fort Worth Tx.
Please see our website at http://www.microscopy.cjb.net/ for
more details.
Schedules and registration will be available at a later
date. Any questions about the
meeting should be directed to Alice Stacey, Program Chair.
Her email address is
kevalc-at-earthlink.net.

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
webmaster for TSM
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed Jan 16 18:24:04 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 16 Jan 2002 19:14:28 -0500
Subject: Re: gold removal from SEM specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To All;

We routinely use a potassium iodide mixture off the shelf made by Acton
Industries, Pennsylvania, USA, on GaAs semiconductors. It's called "GE-6". I
imagine for "gold etch." The pure Au metal is ~ 3.5 uM and is removed
cleanly by this material. The only caveat is that it attacks exposed GaAs
very quickly.

If anyone needs details, please contact me directly.

Peter Tomic
Group Leader
Failure Analysis & Analytical Services
Anadigics, Inc.
Warren, New Jersey

-----Original Message-----
} From: David Henriks [mailto:henriks-at-southbaytech.com]
Sent: Monday, January 07, 2002 3:41 PM
To: c.jeffree-at-ed.ac.uk
Cc: Microscopy Listerver


Chris:

A similar question arose on another listserver, although it was removing
a 2 mciron gold metallizton from GaAs. The responses from there were
as follows:

Response 1
"Greetings!

It is possible that a cut or buffered aqua regia might do the trick. I
have had some success with the recipe below on Au
metallization at M2 and above on GaAs dice. The interlayer dielectric
was silicon dioxide, so there was not a lot of seepage into the
die substrate layer - this, more than the recipe, might have kept the
damage down. Assuming SiO2 as the ILD on all layers of your
part, this might still work:

50 ml deionized water

30 ml HCl

20 ml HNO3

Swirl or agitate the part in the solution for five minutes. Inspect and
repeat once if needed.

One minute rinse in deionized water, followed by 30 seconds rinse in
acetone. Dry under heat lamp to minimize surface staining.

As always, try this on a practice part first in case this does not
buffer the reaction with GaAs enough.

Good Luck !

Regards,

Carl Nail
National Semiconductor
Carl.Nail-at-nsc.com"

Response 2
"A solution of potassium iodide and iodine in water is a decent gold
etch. I have no idea how it would affect GaAs, but I suspect it would
be less destructive than Aqua Regia.

Alan Street
alan-at-irsi.com"

I hope this helps!

David

Chris Jeffree wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
}
} I have been asked for methods of removing the gold sputtered
} coating from
} polished geological specimens that have been used as SEM
} specimens. I have suggested washing with mercury, or alkaline
} sodium cyanide solution, or ammonium thiocyanate solution.
}
} Are there any other methods which would be preferable?
}
} Best wishes, and Happy New Year
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Wed Jan 16 20:54:40 2002



From: Oliver Hockenhull :      hereticfilms-at-shaw.ca
Date: Wed, 16 Jan 2002 18:44:54 -0800
Subject: video footage request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am a media artist based in Canada. I am presently working on a low
budget, art funded DVD project destined for non-profit art gallery use.

The work is a philosophic and poetic look at evolution and bio-diversity.

I am very interested in getting and using some of the video members may
have made on life forms such as viruses, bacteria, etc.

I would be also interested in
acquiring from you a high quality beta-sp or preferable dv tape you might
have of these studies as well as other studies you may have of microscopic
life forms/elements dna, diatoms, etc. Full credit to your organization
(and the videographer if desired) would be given in the final piece. I
would pay for all shipping and handling costs.

The video and images would be a small component of a much longer work.

My last work recently screened at the Canadian Embassy in Washington, D.C.
and I have had shows at the Museum of Modern Art in New York City and at
many other prestigious venues around the world. I include my bio. below.

Please get back to me regarding my request.


Best Wishes,
Oliver Hockenhull
Contact: hereticfilms-at-shaw.ca



From daemon Thu Jan 17 00:11:41 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 17 Jan 2002 01:04:29 -0500
Subject: Market research firm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Val Moshkovskiy wrote:
==========================================================
Can anyone recommend a market research firm or consultant to conduct a
study on an optical instrument to be used in microscopy.
============================================================
We have used in the past the following group:

Microscopy/Marketing & Education
125 Paridon Street, Suite 102
Springfield, MA 01118-2140
PH: (413) 746-6931
FX: (413) 746-9311
Email: mme-at-map.com

Our contact there is Ms. Barbara Foster. We have no financial interest in
this firm, we are just a satisfied client.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Thu Jan 17 05:36:05 2002



From: Anaspec :      anaspec-at-icon.co.za
Date: Thu, 17 Jan 2002 13:24:37 +0200
Subject: ICEM 15, Durban South Africa 1 - 6 September ***Reminder****

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all
A good new year to all from all of us down here in sunny South Africa.

News on the 15th International Conference on Electron Microscopy ( ICEM 15 )
to be held in South Africa, is that things are well in hand. The Scientific
Programme is filling up very quickly and promising to be very interesting.
( http://www.icem15.com/Scientific%20Prog.htm )

Tourists to the country will also be glad to hear that the South African
currency is moving in their favour !!!

Just for those who have not heard of ICEM before, this conference does not
only cover electron microscopy but all disciplines of microscopy. Come along
and see for your self.

We are also pleased that the world Earth Summit, Johannesburg, has
rescheduled their conference to the week before ICEM 15 and not in the same
week as was planned. This conference will attract an estimated 60,000
delegates to Johannesburg, South Africa, which would have made flight
bookings a bit of a problem.( For more on this summit
http://www.joburgsummit2002.com/ ) However we do suggest that you do get
your booking done as soon as possible as most delegates of the earth summit
are sure to visit Durban just to see the beaches. Your travel bookings can
be done via the Turners website (
http://www.turners.co.za/icem15/default.htm ) who are the conference
organisers and travel agents.

However, the main reason for the email is as a reminder that abstracts must
be in by February 2002. Yes, you should panic, that is only a few weeks
away. Please make sure you get them in on time.
If you are having any problems please consult with the relevant people for
your session.

Should you have any further questions, queries or simply would like to see
the current scientific programme, please consult www.icem15.com website for
more details .

Any suppliers still interested in exhibiting should also contact us fairly
quickly, as there are not many stands left.

Best regards and look forward to seeing all 1500, and more, of you at the
beach in Durban.

Luc Harmsen

Marketing ICEM15



From daemon Thu Jan 17 07:25:14 2002



From: ramos-at-argo-tech.com
Date: Thu, 17 Jan 2002 08:12:57 -0500
Subject: SEM. polaroid film type 52

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


M.H. Kish

We utilize both digital photography and type 52 polariod in our
metallographic and SEM lab areas. We buy the type 52 film (by the case)
from:
Laube Photo and Digital Imaging
151 West Exchange Street
Akron, OH 44302
1-800-395-2748



Kelly A. Ramos
Argo-Tech Corporation
Materials Laboratories
Metallurgical Engineer/Supervisor
23555 Euclid Avenue
Cleveland, OH 44117
216-692-5904 (or 216-692-5446)
FAX---} 216-692-5816


Dear Colleagues;
We have a HITACHI SEM. We use Polaroid Type 52 for making pictures. Is
there any
replacement for this type of film without replacing the holder on the
microscope? Where one can buy this type of film? Type 52 seems to be very
rare and it can not be found in the
market.
M. H. Kish
mhkish-at-cic.aku.ac.ir




From daemon Thu Jan 17 07:25:15 2002



From: Jaakko Keranen :      jaakko.keranen-at-tut.fi
Date: Thu, 17 Jan 2002 15:16:14 +0200
Subject: Summer School on Electron Crystallography and Tomography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

The 8th Euro School on Electron Crystallography, 7 - 12 June 2002, Tampere,
Finland

The School will consist of a series of tutorial lectures at a level suited
to graduate students and others wanting to explore the principles of
electron crystallography and tomography. The topics of the School are as
follows: high resolution electron microscopy and image simulation,
quantitative electron diffraction, crystallographic image processing,
convergent beam electron diffraction, Fourier synthesis, direct methods for
crystal structure refinement, and electron tomography of virus structures.
Practical software training is an essential part of the School. The
participants are welcome to present posters.

Applications should be sent before the 15th of February 2002. The number of
participants is limited to 50. You can use on-line registration at the
homepage of Electron Crystallography School,
http://conferences.tut.fi/ecschool2002. For further information, please
contact the Conference Secretary of Electron Crystallography School 2002:
Ms. Minnamari Vippola, Tampere University of Technology, Centre for
Electron Microscopy, e-mail: minnamari.vippola-at-tut.fi

Further information on electron crystallography in the Finnish wilderness
can be found by clicking on the website
http://www.conferences.tut.fi/ecschool2002

***********************************************************************************
Jaakko Keränen

Research Fellow

Tampere University of Technology
Institute of Materials Science
Centre for Electron Microscopy
P.O. Box 589
FIN-33101 Tampere
FINLAND

Phone +358-(0)3-3115 3562
Fax. +358-(0)3-3115 2330
Email jaakko.keranen-at-tut.fi
http://www.tut.fi/units/ms/elm/enindex.htm

The 8th Euro Summer School on Electron Crystallography will be held in
Murikka, Finland, on 7 - 12 June 2002.

Log on to http://www.conferences.tut.fi/ecschool2002/

The 53rd meeting of The Scandinavian Society for Electron Microscopy
SCANDEM 2002 will be held in Tampere, Finland, on 12 - 15 June 2002

Log on to http://www.scandem2002.tut.fi/
***********************************************************************************



From daemon Thu Jan 17 08:04:02 2002



From: Zulal Misirli :      zmisirli-at-boun.edu.tr
Date: Thu, 17 Jan 2002 07:54:15 -0600
Subject: About ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear sirs,
Would you give me some information about observation of polymer
emulsions by ESEM-FEG .
I could saw something at crosssectioned(fractured in liquid nitrogen)
samples( a water-soluble substance was considered as a surface
activator is not seen so good as a network.
I have not cryogenic specimen preparation system.
Thank you for your help.
Sincerelly,
Zulal Misirli


From daemon Thu Jan 17 09:25:36 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 17 Jan 2002 10:14:00 -0500
Subject: RE: TEM collagen fibrils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The skeletal muscle folks always fix in situ or with extirpated muscle under
slight tension (by pinning) to prevent tetanic collapse of sarcomeres during
fixation. This effect is established rather quickly after fixation is
begun.

If one looks at the CT fibers in air-dried (on the slide and stained with
Gomori's aldehyde fuchsin followed by H&E or one of the common blood dyes)
mesentery with BrtFld LM, for example, one will note that the magenta (from
Gomori) elastic fibers run straight while the collagen bundles commonly run
'wavy'. So, to the point.

It would appear that elastin is generally stretched and collagen is normally
NOT under tension in those tissues in which one finds randomly organized
loose connective tissue (LCT) or even the 'woven' fibrous CT of dermis.
Again, to the point.

My suggestion to someone who is trying to normalize the appearance of
collagen filaments, fibrils and/or fibers would be to insure that every
tissue is under equivalent, circumferential tension during fixation. Given
the 'wavy', relaxed(?) condition of collagen bundles in most non-tendinous,
non-ligamentous CT, I would exert sufficient tension on the subject tissues
to insure that the fibers themselves were under tension (i.e., slightly
stretched). Using a tensometer with a 4x40mm slice of dermis(skin) for
example, one should be able to define the tensile force rise point easily as
the slice is stretched and then determine, microscopically, in semi-thin
sections, those fiber bundles of collagen that were under tension when
fixed. They will be straight.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
The best research
Center for Advanced Scientific Imaging
occurs before work
West Chester University
at the bench.
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu




} ----------
} From: Kevin Mackenzie
} Sent: Wednesday, January 16, 2002 10:29 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM collagen fibrils
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} A colleague is planning to investigate collagen reinforcement in natural
} tissues using the TEM.
} Part of the project is to measure the diameter and spacing of the collagen
}
} fibrils. However they are finding that the preparation of the tissue is
} changing both the diameters and spacing of the fibres.
}
} Has anyone suggestions/comments on how to improve tissue structure or any
} other alternative methods.
}
}
} thanks
}
}
} Kevin
} Electron Microscope unit
} Department of Zoology
} University of Aberdeen
} Aberdeen
} AB24 2TZ
}
} Tel 01224-272847
} Fax 01224-272396
} ------------
} Kevin Mackenzie
} k.s.mackenzie-at-abdn.ac.uk
}
}
}


From daemon Thu Jan 17 10:05:21 2002



From: zubkova lidia :      zubkoval-at-yahoo.com
Date: Thu, 17 Jan 2002 07:58:36 -0800 (PST)
Subject: Quantification of Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi, listers,

Does anyone do quantification of Images
using Co-localization algorithm.Where can I get the
software? Any help would be appreciate.


Dr.Lidia A Zubkova
Pharmacology department
845 Union Ave
University of Tennessee
Memphis, TN 38107
Ph(901) 4486009
e-mail:Lzoubkova-at-utmem.edu

__________________________________________________
} } } } Do You Yahoo!?
} } } } Send FREE video emails in Yahoo! Mail!
} } } } http://promo.yahoo.com/videomail/
} } }
} }
} }
} } _______________________________________

__________________________________________________
Do You Yahoo!?
Send FREE video emails in Yahoo! Mail!
http://promo.yahoo.com/videomail/


From daemon Thu Jan 17 10:28:56 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 17 Jan 2002 08:21:42 -0800 (PST)
Subject: Re: Quantification of Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have been doing alot of fluorescence double immuno localization of
proteins and have found simple image multiplication to be very useful in
showing areas of co-localization. If a pixel has a low value in the one
image and its counterpart in the adjacent image is a high signal or value,
the result of multiplication will give you a low value. But if there is a
good signal at that location in both images the multiplication gives you a
amplified pixel value. These values can very quickly become out of the
range of your image display (255 grey values) so we have been using the
multipication in "the Image Processing Tool Kit" Reindeer Games Inc.. It
does the multiplication and then rescales it to fit the scale of the
original images.

Bob
U of Washington
Seattle

On Thu, 17 Jan 2002, zubkova lidia wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Hi, listers,
}
} Does anyone do quantification of Images
} using Co-localization algorithm.Where can I get the
} software? Any help would be appreciate.
}
}
} Dr.Lidia A Zubkova
} Pharmacology department
} 845 Union Ave
} University of Tennessee
} Memphis, TN 38107
} Ph(901) 4486009
} e-mail:Lzoubkova-at-utmem.edu
}
} __________________________________________________
} } } } } Do You Yahoo!?
} } } } } Send FREE video emails in Yahoo! Mail!
} } } } } http://promo.yahoo.com/videomail/
} } } }
} } }
} } }
} } } _______________________________________
}
} __________________________________________________
} Do You Yahoo!?
} Send FREE video emails in Yahoo! Mail!
} http://promo.yahoo.com/videomail/
}
}



From daemon Thu Jan 17 10:44:10 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 17 Jan 2002 11:32:47 -0500
Subject: FW: Cornea disease Not HO HO

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} ----------
} From: Monson, Frederick C.
} Sent: Thursday, January 17, 2002 11:31 AM
} To: 'WWmn916-at-aol.com'
} Subject: RE: Cornea disease Not HO HO
}
} Not disease, BUT!!!!
}
} I remember an early electron microscopist describing how, as a graduate
} student, he would sit at his dissecting scope watching the course of
} osmium tetroxide fixation. He would stop when his foggy cornea would no
} longer permit him to see anything. He would resume when his cornea
} cleared a couple days later. He did not publish the data he collected on
} corneal renewal, because he didn't think of it as an experiment in
} progress, but only as an experiment delayed.
}
} Oh well, I've done my damage for the day,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} The best research
} Center for Advanced Scientific Imaging
} occurs before work
} West Chester University
} at the bench.
} West Chester, Pennsylvania, USA, 19383
} 610-738-0437
} fmonson-at-wcupa.edu
}
}
} ----------
} From: WWmn916-at-aol.com
} Sent: Wednesday, January 16, 2002 8:43 PM
} To: histonet-at-pathology.swmed.edu
} Subject: Cornea disease
}
} Greetings to all,
} I'm curious if anyone would know if developing cornea disease is
} somewhat common in the lab world? With all the chemicals and fumes we
} work with, I wouldn't be surprised.
}
} Deb King, HT
} Sacramento, CA
}
}


From daemon Thu Jan 17 11:06:16 2002



From: Delilah Wood :      wood-at-pw.usda.gov
Date: Thu, 17 Jan 2002 09:01:40 -0800
Subject: Re: Contact info for Scanning Microscopy International

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick, Actually, Scanning Microscopy International is defunct. Scanning is NOT the same organization.


De Wood


At 11:47 AM 1/16/2002 -0600, Tina Schwach wrote:

} ------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} -----------------------------------------------------------------------.

}

}

} Rick,

} The organization is Scanning and you can find them at www.scanning-fams.org.

}

} Dr.Tina S. Schwach, President

} Microscopy Consulting Services Inc.

} 651-681-0112

} ----- Original Message -----

} } From: Rick Powell at Nanoprobes

} To: microscopy-at-sparc5.microscopy.com

} Sent: Tuesday, January 15, 2002 2:16 PM

} Subject: Contact info for Scanning Microscopy International

}

}

} ------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} -----------------------------------------------------------------------.

}

}

} Hello Microscopists:

}

} I'd like to obtain permission to reproduce a figure from a paper in

} Scanning Microscopy Supplement, Volume 10 (1996), a.k.a the Proceedings of

} the 14th Pfefferkorn Conference. The publisher is listed as Scanning

} Microscopy International, with the following contact information:

}

} Dr. Om Johari

} P.O. Box 66507

} Chicago (A.M.F. O'Hare), IL 60666-0507

} USA

}

} E-mail: 73211.647-at-compuserve.com

} Web: http://hem2.passagen.se/smi/

} Tel: 847-524-6677

}

} But... the phone number has been disconnected, there's no reply to mail or

} e-mail, and the web address gives a "page not found" default (in Swedish);

} searching this ISP gave me no results, and neither do the regular search

} engines (Google, etc.).

}

} Does anyone know what happened to Scanning Microscopy International and how

} to contact them, or who owns the copyright to this publication now?

}

} Thanks in advance,

}

} Rick Powell

}

}

} ****************************************************************************

} *************

} Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com

} NANOPROBES, Incorporated

} 95 Horse Block Road, Yaphank, NY 11980-9710, USA

}

} US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)

} 980-3608

}

} Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html

} ****************************************************************************

} *************

}

}

}

{bold} ********************************************************************

Delilah F. Wood

Botanist

USDA-ARS-WRRC

800 Buchanan St.

Albany, CA 94710


Tel: 510-559-5653

Fax: 510-559-5818 {/bold}


From daemon Thu Jan 17 11:37:25 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 17 Jan 2002 08:31:16 -0800
Subject: Re: SEM. polaroid film type 52

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Kish,
We have always used Polaroid type 55 in our Hitachi SEM, since it also
gives a negative for reproducing the photos. It is slower than the type 52,
so you have to adjust your exposure. As far as we know, it is still
available. However, several years ago we switched our SEM to a digital image
capture system, since it acquires the photo in computer-compatible format,
and we have not used the Polaroid since.
At 06:59 AM 1/16/02 -0600, you wrote:
}
} Dear Colleagues;
} We have a HITACHI SEM. We use Polaroid Type 52 for making pictures. Is
} there any
} replacement for this type of film without replacing the holder on the
} microscope? Where one can buy this type of film? Type 52 seems to be very
} rare and it can not be found in the
} market.
} M. H. Kish
} mhkish-at-cic.aku.ac.ir
}
Regards,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Thu Jan 17 12:30:11 2002



From: Wilson, Nick :      nwilson-at-nrcan.gc.ca
Date: Thu, 17 Jan 2002 13:22:37 -0500
Subject: Anyone used a Gatan PanaCL unit?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We are considering purchasing a Gatan PanaCL detector for our Philips XL30
SEM?
Anybody used one of these and do you have any comments / recommendations?
We would be using it for petrological and paragenetic studied of sandstones
etc.
Thanks
Nick Wilson
Dr. Nick Wilson
Research Scientist
Geological Survey of Canada
3303-33rd St. N.W.
Calgary AB, Canada T2L 2A7
Tel: 403-292-7045
Fax: 403-292-7159
Email: nwilson-at-nrcan.gc.ca {mailto:nwilson-at-nrcan.gc.ca}


From daemon Thu Jan 17 13:18:25 2002



From: Kim Riddle :      riddle-at-bio.fsu.edu
Date: Thu, 17 Jan 2002 14:13:23 -0500
Subject: service providers - Galveston, TX area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

Could someone send me the names and phone #'s of service providers other
than JEOL for service on an 100CX in the Galveston, TX area?

Thanks, Kim
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Kimberly A. Riddle
Florida State University tel: 850.644.6519
Biological Science Imaging Resource
119 Bio Unit I, 4370 fax: 850.644.0481
Tallahassee, FL 32306 riddle-at-bio.fsu.edu
http://bio.fsu.edu/~taylor/imaging
~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu Jan 17 15:20:03 2002



From: DrJohnRuss-at-aol.com
Date: Thu, 17 Jan 2002 16:11:34 EST
Subject: Re: Quantification of Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 1/17/02 11:07:24 AM, zubkoval-at-yahoo.com writes:

} Does anyone do quantification of Images
} using Co-localization algorithm.Where can I get the
} software? Any help would be appreciate.

Two of the software packages that I know contain that functionality are Image
Pro Plus version 4.5 (http://www.mediacy.com) and Fovea Pro
(http://reindeergraphics.com). Probably there are many others as well.


From daemon Thu Jan 17 17:03:37 2002



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu
Date: Thu, 17 Jan 2002 16:55:44 -0600
Subject: RE: SEM. polaroid film type 52

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } Dear Colleagues;
} } We have a HITACHI SEM. We use Polaroid Type 52 for making pictures. Is
} } there any
} } replacement for this type of film without replacing the holder on the
} } microscope? Where one can buy this type of film? Type 52 seems to be very
} } rare and it can not be found in the
} } market.
} } M. H. Kish
} } mhkish-at-cic.aku.ac.ir


Try http://www.ngscorp.com/photo_film.php
We have ordered Polaroid film from them in the past, so my only connection is
as a customer (insert financial interest disclaimer here).
Randy



From daemon Thu Jan 17 17:03:38 2002



From: Linda Jenkins :      lcjenkins-at-asub.arknet.edu
Date: Thu, 17 Jan 2002 16:49:30 -0600
Subject: Training for Optical Microscope Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to be trained to maintain and repair optical microscopes that are
used in biology, microbiology, and A & P classes. Where is such training
available?
Thank-you,
Linda




From daemon Thu Jan 17 21:26:14 2002



From: DAS :      metallurgy-at-skynet.be
Date: Wed, 16 Jan 2002 18:57:00 +0100
Subject: Measuring microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An interest exists in measuring cross sections of tubes, wires, etc
(diameters, thickness). I would deeply appreciate if you submit some
information on supplies and any existing experience.

Thanking you in advance,

Dr Dimitri ASLANIDIS
AMS World Services
rue de la Chaussee 1, 1320 Beauvechain, Belgium
tel +32 478 296969, fax +32 10 862134



From daemon Thu Jan 17 21:41:12 2002



From: mtl :      mtl-at-njcc.com
Date: Thu, 17 Jan 2002 22:39:00 -0500
Subject: Re: SEM. polaroid film type 52

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We no longer use Polaroid Type 52 in our lab. We found that Polaroid Type 72
gives better exposure range and doesn't require coating. Our last bulk film
purchase was from FilmAce (www.filmace.com) who advertised in Microscopy
Today. However, they also sell Type 52. Usual customer disclaimers.
Roy Nelson
Material Testing Lab.
mtl-at-njcc.com

a7528922 wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Colleagues;
} We have a HITACHI SEM. We use Polaroid Type 52 for making pictures. Is
} there any
} replacement for this type of film without replacing the holder on the
} microscope? Where one can buy this type of film? Type 52 seems to be very
} rare and it can not be found in the
} market.
} M. H. Kish
} mhkish-at-cic.aku.ac.ir



From daemon Fri Jan 18 07:07:57 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Fri, 18 Jan 2002 07:58:50 -0500
Subject: Re: About ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Zulal - the hardest part is sample preparation - as the biology guys know
from many years of experience, cryo preparation is fraught with freezing
artifacts. There is a large body of literature about freeze
fracture/freeze etch that you can peruse for the myriad of artifacts and
pitfalls associates with the technique. You certainly do not need an ESEM
- indeed, you probably do not even want to use an ESEM - to do this - it
will only make a difficult task harder.

Briefly, the sample must be frozen extremely rapidly - you'll need a jet
freezer, spray freezer, diamond anvil slammer or high pressure freezer -
even then the most artifact free material you can expect is less than
500um and most of the techniques yield good samples only in the 15-25um
range. The sample must be kept below -100C or the amorphous ice will change
to crystalline ice and ruin your samples. You will,of course have to have a
cold stage in your microscope and a good cry transfer system to do all of
this.


Bill Miller


At 08:54 AM 1/17/02, Zulal Misirli wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Jan 18 07:45:20 2002



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Fri, 18 Jan 2002 08:38:31 -0500
Subject: 3-D reconstruction software recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

We are looking for a relatively simple (inexpensive) 3-D reconstruction
software package for Mac or PC. Can anyone recommend some software?

Thanks
Henk Colijn


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Fri Jan 18 08:34:50 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 18 Jan 2002 07:28:20 -0700
Subject: Re: Quantification of Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just for the record:

The mFIP (multiple Fluorescence Image Processing) module in our analySIS
software also includes colocalization.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "DrJohnRuss-at-aol.com"-at-sparc5.microscopy.com
[mailto:"DrJohnRuss-at-aol.com"-at-sparc5.microscopy.com]
Sent: Thursday, January 17, 2002 2:12 PM
To: zubkoval-at-yahoo.com; Microscopy-at-sparc5.microscopy.com



In a message dated 1/17/02 11:07:24 AM, zubkoval-at-yahoo.com writes:

} Does anyone do quantification of Images
} using Co-localization algorithm.Where can I get the
} software? Any help would be appreciate.

Two of the software packages that I know contain that functionality are
Image
Pro Plus version 4.5 (http://www.mediacy.com) and Fovea Pro
(http://reindeergraphics.com). Probably there are many others as well.


From daemon Fri Jan 18 09:50:25 2002



From: heiko.stegmann-at-amd.com
Date: Fri, 18 Jan 2002 16:41:20 +0100
Subject: RE: 3-D reconstruction software recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Henk,

which particular 3D reconstruction technique are you referring to?
Reconstruction from serial sections, single axis tilt series, randomly tilted single particles... ????

-Heiko



-----Ursprüngliche Nachricht-----
Von: Hendrik O. Colijn [mailto:colijn.1-at-osu.edu]
Gesendet: Freitag, 18. Januar 2002 14:39
An: Microscopy-at-sparc5.microscopy.com
Betreff: 3-D reconstruction software recommendations

Hi all,

We are looking for a relatively simple (inexpensive) 3-D reconstruction
software package for Mac or PC. Can anyone recommend some software?

Thanks
Henk Colijn


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.




From daemon Fri Jan 18 13:06:28 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 18 Jan 2002 10:58:32 -0800
Subject: SEM of feces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This topic came up earlier but did not come to
a conclusion, as I recall. I subsequently found some references
to SEM of skunk and opossum feces at several
veterinary sites. Their purpose was to examine the
animal feces for remnants of honey bees. Apparently,
these nocturnal mammals raid bee hives and eat the
bees.

I did not find any references to SEM of human feces.
From what I can see, there are basically two types
of feces. One is of a slime nature while the other
is more compacted and solid. The nature is basically
determined by its location in the sequence of expulsion.

I collected both types of specimens (slime on Al stub,
compacted on C stub) and began working with
the slime type. Both were vacuum desiccated at 40mT for
4 hours. After this, the slime specimen was coated with
60A of Pt. The specimen was placed in high vacuum SEM
chamber and allowed to reach about 2E-8T.

The images were rather boring. The set of images taken
can be found at:

http://photoweb.net/feces/Catalog.pdf

This PDF file is about 3.9MB in size. Individual files
can be uploaded but I did not do this yet.

From the images, it is clear that the slime cracks
severely under vacuum. Some of the gaps are between
1-5um. Even with 60A of coating, some "islands"
of slime charge. Thus, it would seem that 60A is
not enough coating to prevent charging. I did some
other images (in the PDF catalog) which were BSE.

Even so, there does not seem to be anything
remarkable about this specimen. This is in stark
contrast to LM analysis using BF, DIC, phase and DF.
The problem with LM is that the bacteria are motile and
cannot effectively be photographed--due to too long
of exposure time. If I cool the specimen, I can approach
useable images, since the bacterias' movement slows
appreciably. But the limited depth of field does not
make are particularly great image.

In the SEM, there simply is not much to discern.
I suspect that part of the problem is that I am not
able to prepare these specimens as biological types.
I am fundamentally set up for metallurgical work.

The specimens should be rinsed, filtered, fixed and
then mounted. Without fixation, the water in the
bacteria is removed and the structure/morphology
of the bacteria is destroyed. Additionally, without
removing the indigenous liquid material, one cannot
see the bacteria anyway, since they are below the
surface of the liquid. This is where filtering would
help. I've had this same problem with samples of
pus and of spider blood. The fluid is so thick compared
to any solid object that might be in the fluid, all
that is seen is the surface of the fluid. The LM,
as would a TEM, can see through the fluid. The
SEM does not.

My conclusion is that for my particular setup,
SEM of feces is not possible--or at least, not
useful. If anyone has any alternative ideas about
SEMing feces, I'd appreciate hearing them.
If it is a worthless effort, I'd appreciate knowing that too.

tnx,
Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA
916.791.8191



From daemon Fri Jan 18 13:27:30 2002



From: John Sutko :      sutko-at-med.unr.edu
Date: Fri, 18 Jan 2002 11:29:38 -0800
Subject: Antibodies against human cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We need an antibody or antibodies, preferably against a cell surface antigen,
that recognizes cells of human origin specifically (i.e. does not stain cells
from other mammalian species), and which works for immuno-fluorescence. We
would like to investigate a wide range of cell types from different organs. We
have made enquires with many companies, spent a significant amount of money, but
have yet to find a suitable antibody. Thanks in advance for any and all
suggestions. This message has been sent to several lists, apologies for any
duplication.

John

John Sutko
Department of Pharmacology/318
Univ. Nevada, Reno
Reno, NV 89557

Tel. (775) 784-4121, -4537
Fax (775) 784-1620
email sutko-at-unr.edu




From daemon Fri Jan 18 13:58:26 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 18 Jan 2002 11:52:03 -0800 (PST)
Subject: Re: 3-D reconstruction software recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Imod is for linux, runs nicely on a PC, and is free.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Fri, 18 Jan 2002, Hendrik O. Colijn wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
}
} We are looking for a relatively simple (inexpensive) 3-D reconstruction
} software package for Mac or PC. Can anyone recommend some software?
}
} Thanks
} Henk Colijn
}
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://www.ceof.ohio-state.edu
} Fools are pleased when they discover error.
} The wise are pleased when they discover truth.
}
}



From daemon Fri Jan 18 15:49:15 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Fri, 18 Jan 2002 16:39:13 -0500
Subject: RE: 3-D reconstruction software recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used Slicer for 3D reconstruction of tif images. Slicer will
automatically read in a series of tif images that contain a sequential ID
number in their name and you can set the separation distance between each
image for display. Slicer will also make AVI movies of zooms and rotations.
Everett Ramer
Cellomics, Inc.
Pittsburgh, PA

-----Original Message-----
} From: "heiko.stegmann-at-amd.com"-at-sparc5.microscopy.com
[mailto:"heiko.stegmann-at-amd.com"-at-sparc5.microscopy.com]
Sent: Friday, January 18, 2002 10:41 AM
To: Microscopy-at-sparc5.microscopy.com


Hi Henk,

which particular 3D reconstruction technique are you referring to?
Reconstruction from serial sections, single axis tilt series, randomly
tilted single particles... ????

-Heiko



-----Ursprüngliche Nachricht-----
Von: Hendrik O. Colijn [mailto:colijn.1-at-osu.edu]
Gesendet: Freitag, 18. Januar 2002 14:39
An: Microscopy-at-sparc5.microscopy.com
Betreff: 3-D reconstruction software recommendations

Hi all,

We are looking for a relatively simple (inexpensive) 3-D reconstruction
software package for Mac or PC. Can anyone recommend some software?

Thanks
Henk Colijn


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.




From daemon Fri Jan 18 15:51:19 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Fri, 18 Jan 2002 16:43:56 -0500
Subject: RE: 3-D reconstruction software recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used Slicer for 3D reconstruction of tif images. Slicer will
automatically read in a series of tif images that contain a sequential ID
number in their name and you can set the separation distance between each
image for display. Slicer will also make AVI movies of zooms and rotations.
Everett Ramer
Cellomics, Inc.
Pittsburgh, PA

-----Original Message-----
} From: "heiko.stegmann-at-amd.com"-at-sparc5.microscopy.com
[mailto:"heiko.stegmann-at-amd.com"-at-sparc5.microscopy.com]
Sent: Friday, January 18, 2002 10:41 AM
To: Microscopy-at-sparc5.microscopy.com


Hi Henk,

which particular 3D reconstruction technique are you referring to?
Reconstruction from serial sections, single axis tilt series, randomly
tilted single particles... ????

-Heiko



-----Ursprüngliche Nachricht-----
Von: Hendrik O. Colijn [mailto:colijn.1-at-osu.edu]
Gesendet: Freitag, 18. Januar 2002 14:39
An: Microscopy-at-sparc5.microscopy.com
Betreff: 3-D reconstruction software recommendations

Hi all,

We are looking for a relatively simple (inexpensive) 3-D reconstruction
software package for Mac or PC. Can anyone recommend some software?

Thanks
Henk Colijn


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.




From daemon Fri Jan 18 17:08:56 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 18 Jan 2002 14:58:43 -0800
Subject: Rapid fixer yellow precipitate?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Before old time photography fades from the scene completely and no one on
this list remembers it, I have a question I have been curious about for a
while.

Sometimes, after a long time in storage, a yellow precipitate forms in some
containers of Rapid Fix. I just noticed it again in a bottle of Mohr
Profix, similar formula to Rapid Fix, I think.

What is this stuff and why does it form? It is pale yellow, sticks mostly
to the bottom of the bottle. It is hard to clean off and smells a little
sulphurish.

Is there any way to clean it out of the bottle, if I do, is it more toxic
than ordinary fixer?

Inquiring minds want to know.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Sat Jan 19 02:01:10 2002



From: Ken Gaugler :      ken-at-gaugler.com
Date: Fri, 18 Jan 2002 23:51:50 -0800
Subject: Re: Rapid fixer yellow precipitate?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jonathan,

The yellow precipitate is probably elemental sulfur. Fixer's main ingredient is
sodium thiosulfate, which over time can get oxidized. You can filter the stuff
out, but IMHO if your rapid fixer is that deteriorated, you should probably
toss it. Using bad fixer can cause stains to appear on your prints after a few
weeks or months.

Best Regards,
Ken Gaugler

Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi:
}
} Before old time photography fades from the scene completely and no one on
} this list remembers it, I have a question I have been curious about for a
} while.
}
} Sometimes, after a long time in storage, a yellow precipitate forms in some
} containers of Rapid Fix. I just noticed it again in a bottle of Mohr
} Profix, similar formula to Rapid Fix, I think.
}
} What is this stuff and why does it form? It is pale yellow, sticks mostly
} to the bottom of the bottle. It is hard to clean off and smells a little
} sulphurish.
}
} Is there any way to clean it out of the bottle, if I do, is it more toxic
} than ordinary fixer?
}
} Inquiring minds want to know.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu

--
Ken Gaugler
Santa Clara, CA.
~~ spark: N6OSK ~~
PGP: 2EF7 437E 1D0B 602D BC2C 92D1 A548 C11B B901 8CDE





From daemon Sat Jan 19 07:44:22 2002



From: Samuel Purdy :      spurdy52-at-mac.com
Date: Sat, 19 Jan 2002 07:32:09 -0600
Subject: Rapid fixer yellow precipitate?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello!
It is elemental sulphur. It is also a signal that your rapid fix is
beginning to decompose and is losing its strenth. It sould be dumped.

Sam Purdy
spurdy52-at-earthlink.net


From daemon Sat Jan 19 07:44:22 2002



From: Edward_Principe-at-amat.com
Date: Sat, 19 Jan 2002 07:32:29 -0600
Subject: 3-D reconstruction software recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



VolumeJ is an add-in to ImageJ, which is the PC version of NIH image. It
is free. Scan the web, you'll find it.

OpenDX is a unix free software.

Matlab has volume reconstruction capabilities that are quite nice.

Regards,
Ed


"Hendrik O. Colijn" {colijn.1-at-osu.edu} on 01/18/2002 05:38:31 AM


To: Microscopy-at-sparc5.microscopy.com
cc:


Hi all,

We are looking for a relatively simple (inexpensive) 3-D reconstruction
software package for Mac or PC. Can anyone recommend some software?

Thanks
Henk Colijn


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.


From daemon Mon Jan 21 08:16:00 2002



From: SJT-at-NP.EDU.SG ()
Date: Mon, 21 Jan 2002 07:54:44 -0600
Subject: Ask-A-Microscopist: ophthalmoloscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (SJT-at-NP.EDU.SG) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
January 21, 2002 at 02:54:09
---------------------------------------------------------------------------

Email: SJT-at-NP.EDU.SG
Name: Jeanette T. Sasam

Organization: NgeeAnn Polytechnic

Education: Undergraduate College

Location: Singapore

Question: Can an ophthalmoloscope also be considered a microscope?
What is a typical magnification of an Ophthalmoloscope?

---------------------------------------------------------------------------


From daemon Mon Jan 21 08:16:00 2002



From: MIKE.HALE-at-AVON-RUBBER.COM ()
Date: Mon, 21 Jan 2002 07:56:00 -0600
Subject: Ask-A-Microscopist: polymer blends by SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (MIKE.HALE-at-AVON-RUBBER.COM) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
January 21, 2002 at 06:07:56
---------------------------------------------------------------------------

Email: MIKE.HALE-at-AVON-RUBBER.COM
Name: MIKE HALE

Organization: AVON-RUBBER

Education: Graduate College

Location: WESTBURY, WILTS, UK

Question: To study the phase morphology of polymer blends by SEM, we
have been staining thin films with osmium tetroxide solution. The
method we have been using is to simply soak small pieces of film
overnight in a 2% OsO4 solution, wash with water an acetone, gold
coat and view. This seems to work quite well, the samples becoming
darker in colour and the SEM SEI images being usable although of
relatively low contrast.

The materials being studied are blends of saturated and unsaturated
polymers but we have noticed the presence of osmium in both phases,
which is not what we would have expected.

I would be most grateful if you can offer any suggestions regarding
the staining of polymer blends with osmium tetroxide as I feel we are
not getting the best from this technique. Thanking you in advance
for your assistance.

---------------------------------------------------------------------------


From daemon Mon Jan 21 08:16:00 2002



From: Jesse Rodrigues :      Jesse_Rodrigues-at-kopin.com
Date: Mon, 21 Jan 2002 09:05:30 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




From daemon Mon Jan 21 11:45:48 2002



From: Caroline Miller :      camiller-at-creighton.edu
Date: Mon, 21 Jan 2002 11:38:57 -0600
Subject: Knifebreakers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like information about an older Sorvall glass knife breaker
making knifes that are 12-13 mm wide. Caroline Miller



From daemon Mon Jan 21 11:57:22 2002



From: Josh Kahn :      4jbk1-at-qlink.queensu.ca
Date: Mon, 21 Jan 2002 12:51:59 -0500
Subject: TEM Poly-l-Lysine coating sinlge slot grids protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can I use poly-l-lysine against formvar or does it have to be used against a
carbon coat?

JBK
Read books



From daemon Mon Jan 21 13:26:01 2002



From: gary.m.brown-at-exxonmobil.com
Date: Mon, 21 Jan 2002 13:17:39 -0600
Subject: Re: Ask-A-Microscopist: polymer blends by SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Mike,

I have three comments/suggestions for you:
OsO4 staining is selective not only for unsaturation but also for
certain functional groups (i.e. -OH). I suggest that you research the
stain specificity of OsO4 as it applies to the polymers you are
studying. Sawyer and Grubbs "Polymer Morphology" may provide much of the
information you need.
Vapor staining, instead of solution staining, may improve your results.
During solution staining, the OsO4 may diffuse into both phases but
chemically react with only the unsaturated phase. An excess of OsO4 in
your resin may impede diffusion of unreacted OsO4 out of your sample. I
suggest that the sample be vapor stained by attaching it to the inner
surface of the cap for your vial of OsO4 and staining overnight. Be sure
to degas the sample in a nitrogen purge or vacuum to remove all
unreacted OsO4. If the sample is too thick to permit diffusion of OsO4
throughout the film's full thickness, create a cryogenically sectioned
plane through the film and stain this new surface in OsO4 vapors.
Metal coating the stained sample will reduce the differential contrast
created by OsO4 staining. You may opt to examine the stained samples at
low accelerating voltage in a field-emission SEM or in a variable
pressure SEM. Charging of the stained but uncoated polymer can be
controlled using either of these instruments. If you must coat, a thin
layer of carbon should help to minimize charging and optimize the domain
contrast that you desire.

Good luck,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



MIKE.HALE-at-AVON-
RUBBER.COM () To: Microscopy-at-sparc5.microscopy.com
cc:
Subject: Ask-A-Microscopist: polymer blends by
01/21/02 07:56 SEM
AM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (MIKE.HALE-at-AVON-RUBBER.COM) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
January 21, 2002 at 06:07:56
---------------------------------------------------------------------------

Email: MIKE.HALE-at-AVON-RUBBER.COM
Name: MIKE HALE

Organization: AVON-RUBBER

Education: Graduate College

Location: WESTBURY, WILTS, UK

Question: To study the phase morphology of polymer blends by SEM, we
have been staining thin films with osmium tetroxide solution. The
method we have been using is to simply soak small pieces of film
overnight in a 2% OsO4 solution, wash with water an acetone, gold
coat and view. This seems to work quite well, the samples becoming
darker in colour and the SEM SEI images being usable although of
relatively low contrast.

The materials being studied are blends of saturated and unsaturated
polymers but we have noticed the presence of osmium in both phases,
which is not what we would have expected.

I would be most grateful if you can offer any suggestions regarding
the staining of polymer blends with osmium tetroxide as I feel we are
not getting the best from this technique. Thanking you in advance
for your assistance.

---------------------------------------------------------------------------







From daemon Tue Jan 22 02:11:41 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 22 Jan 2002 03:00:12 -0500
Subject: Staining of polymers for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Mike Hale wrote:
=======================================================
Question: To study the phase morphology of polymer blends by SEM, we have
been staining thin films with osmium tetroxide solution. The method we
have been using is to simply soak small pieces of film overnight in a 2%
OsO4 solution, wash with water an acetone, gold coat and view. This seems
to work quite well, the samples becoming darker in colour and the SEM SEI
images being usable although of relatively low contrast.

The materials being studied are blends of saturated and unsaturated
polymers but we have noticed the presence of osmium in both phases, which
is not what we would have expected.

I would be most grateful if you can offer any suggestions regarding the
staining of polymer blends with osmium tetroxide as I feel we are not
getting the best from this technique. Thanking you in advance for your
assistance.
=============================================================
We have found over the years that in general, TEM is light years better than
the best SEMs for the characterization of the multiphase structure of
modified polymer systems.

When staining polymers, remember that just about any polymer, if left in the
presence of osmium tetroxide, will eventually turn black. What makes the
osmium approach work is that some phases stain far faster than other phases.
A good rule of thumb is that if you want to increase the contrast between
the two phases, slow down the staining, either by reducing the concentration
(if vapor phase, then the partial pressure) of the osmium tetroxide or by
lowering the staining temperature.

If one must use only an SEM, then we have found that we

a) first make a "faced off piece" of the sample with a diamond knife in a
cryo stage ultramicrotome, thereby making the smoothest possible surface,

b) vapor stain the system with osmium tetroxide (or ruthenium tetroxide
depending on the chemistry of the sample). However if it is looked at by
SEM at this point, there can be back ground staining of the unstained
portions of the sample. The next step then is to

c) oxygen plasma etch the sample in a simple RF plasma etcher, such as our
SPI Plasma Prep II (see our website given below), using oxygen. The oxygen
will etch down the unstained portion of the sample and will etch far more
slowly the osmium (or ruthenium) stained portions of the sample. One need
not etch very long but this does produce enough additional topography to
result in acceptable contrast whereby, without etching, there was
insufficient contrast.

d) We think that coating with osmium metal (in the OPC osmium coater, see
URL
http://www.2spi.com/catalog/osmium-plasma-coater-opc-60.html) instead of
gold or carbon has some advantages. One might normally think that a heavy
metal is not the way to go, but the layer is much thinner and for BSE
imaging, it can have some advantages, for example, see URL
http://www.2spi.com/catalog/osmium-plasma-coater-demonstration.html This is
speculation on my part, but it is based on the experience with osmium
coating of immunogold tagged cells.


The main problem with SEM approaches is that if matrix inclusions are
present, they tend to be small, and are often times missed, whereas by TEM,
they would be seen readily.

Disclaimer: SPI Supplies offers the Plasma Prep II plasma etcher and the
OPC-60 Osmium Plasma Coater. Our laboratory services division performs
polymer electron microscopy on these kinds of samples for commercial clients

From daemon Tue Jan 22 03:20:18 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Tue, 22 Jan 2002 10:14:00 +0100
Subject: Multi-photon microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Here at our lab, I managed to generate a 3D image with a Zeiss LSM510
confocal microscope in tissue with a thickness of about 400 micrometer. The
deepest optical slice I could scan was about 250 - 300 micrometer. This was
in lung tissue. Now I know depth of scanning is dependant of the tissue
density, magnification etc.

In the future, we would like to make scans in pig heart tissue, but I think
that with our confocal, I wouldn't be able to scan deeper than about 200 -
250 micrometer. Now we're looking for a solution to scan through the whole
heart-wall, to detect a fluorophore coupled to the Y-chromosone.

With a multiphoton confocal microscope, it is possible to scan deeper, also
in combination with a non-descanned detector even deeper. But how deep,
with what staining etc.? And the final question: has anyone any experience
with scanning through pig heart tissue, and, how deep were you able to scan?
It is very important for us, because we're thinking about buying such a
multi-photon confocal microscope, but we should be sure before spending the
money!

Thanks in advance,

Sven

___________________________________________
Sven Terclavers
Research Assistent
Center for Molecular and Vascular Biology
Center for Transgene Technology and Gene Therapy
Campus Gasthuisberg O/N
Herestraat 49
3000 Leuven - Belgium
___________________________________________



From daemon Tue Jan 22 08:53:49 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 22 Jan 2002 09:37:51 -0500
Subject: RE: Knifebreakers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Sorvall knifebreaker was manufactured 'in the same breath' as the JB-4
to provide the wide glass knives that would cut small specimens embedded in
GMA. The knife breaker was capable of cutting quite good knives from 6mm
glass for routine thin sectioning. As far as I am aware, the one I bought
in 1983(?) is still working.

Fred Monson

Frederick C. Monson, PhD
The best research
Center for Advanced Scientific Imaging
occurs before work
West Chester University
at the bench.
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


} ----------
} From: Caroline Miller
} Sent: Monday, January 21, 2002 12:38 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Knifebreakers
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would like information about an older Sorvall glass knife breaker
} making knifes that are 12-13 mm wide. Caroline Miller
}
}
}


From daemon Tue Jan 22 09:00:21 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 22 Jan 2002 06:55:18 -0800 (PST)
Subject: Re: Rapid fixer yellow precipitate?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I think you are correct in the "smell test" it is sulphur from the
ammonium thio(sulphate).

Bob
Old Timer

On Fri, 18 Jan 2002, Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} Before old time photography fades from the scene completely and no one on
} this list remembers it, I have a question I have been curious about for a
} while.
}
} Sometimes, after a long time in storage, a yellow precipitate forms in some
} containers of Rapid Fix. I just noticed it again in a bottle of Mohr
} Profix, similar formula to Rapid Fix, I think.
}
} What is this stuff and why does it form? It is pale yellow, sticks mostly
} to the bottom of the bottle. It is hard to clean off and smells a little
} sulphurish.
}
} Is there any way to clean it out of the bottle, if I do, is it more toxic
} than ordinary fixer?
}
} Inquiring minds want to know.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}



From daemon Tue Jan 22 13:19:42 2002



From: Jo Ann Moore :      jamoore-at-hsc.usf.edu
Date: Tue, 22 Jan 2002 14:06:14 -0500
Subject: need 24 well glass culture plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:

A grad student in the Anatomy department is looking for glass 24 well
tissue culture plates however they are only made of plastic now. Does
anyone here at the university have some in their lab that our student
could borrow? Or does anyone know of a source from which to purchase
them?

Please contact me by email or phone if you can help.
Thanks,

Jo Ann Moore
Sr. Biological Scientist
Anatomy Dept.
4 x9446





From daemon Tue Jan 22 22:37:11 2002



From: Cavin Mooers :      cavinm-at-vsl.cua.edu
Date: Tue, 22 Jan 2002 22:24:50 -0600
Subject: EM Position Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Research Assistant, Electron Microscopist
Vitreous State Laboratory, Washington, D.C.


Appointment Date: 20 May, 2002

Salary and Benefits: 36,000 to 39,000, 2x matching 401k, (up to 5% of
salary after 1 year,) 21 days paid vacation, 15 days paid holiday, sick
leave, health and life insurance, flexible spending account for
medical/dental reimbursement, tuition reimbursement, (up to 6 credits
per semester,) 5% pay raise after 3 months, (dependent upon positive
review,) and relocation assistance.

Essential Duties: Microstructural characterization of materials
utilizing OLM, SEM, EDS, WDS, TEM, STEM, and XRD. Provide technical
expertise to researchers on equipment operation and microscopy
laboratory protocols. Conduct basic EM maintenance on related
equipment.

Qualifications: Candidate must either have a two-year degree in
electron microscopy, or a BS in physics, chemistry, or materials
science with three years of electron microscopy experience. Candidate
must have operational knowledge of scanning and transmission electron
microscopes, as well as, energy-dispersive spectroscopy systems,
fundamental understanding of image processing and analysis techniques,
capacity to prepare SEM specimens utilizing standard metallographic
techniques, ability to prepare TEM specimens via tripod polishing, jet
polishing, dimpling/ion milling, and extraction replication, a
generally good understanding of electron microscopy lab maintenance,
and strong verbal and written communication skills. Knowledge of FTIR
and Raman spectroscopy operations considered a bonus.

Instrumentation: JEOL 5900LV and 35c SEM’s, JEOL 2000EX / FX TEM,
Noran Vantage and Oxford INCA ENERGY 300/WAVE 700 EDS/WDS systems,
Olympus upright light microscope w/ brightfield/darkfield/polarizing
light, Olympus inverted stage w/
brightfield/darkfield/polarizing/Nomarski’s.


From daemon Wed Jan 23 08:59:34 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Wed, 23 Jan 2002 09:44:15 -0500
Subject: RE: Knifebreakers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Entire Sorvall, JB-4 Microtomy system for plastics (and paraffin) was
purchased by Energy Beam Sciences around 1990 and we still offer that line
today. It consists of:

1. JB-4(Manual) and JB-4A(Automatic) Microtomes
2. GKM Triangular Knifemaker, for making knives up to 12 mm wide that are
used for thin sectioning.
3. Ralph Knifemaker, for long knives up to 38 mm wide that are used for
semi-thin paraffin or resin embedded sections.

Contact EBSciences for any product, service or accessory information you
need at (800) 992-9037 or via email at ebs-at-ebsciences.com.

Best regards,

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
www.ebsciences.com
"Adding Brilliance to Your Vision"






-----Original Message-----
} From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
Sent: Tuesday, January 22, 2002 9:38 AM
To: 'Caroline Miller'
Cc: 'List-Microscopy'


The Sorvall knifebreaker was manufactured 'in the same breath' as the JB-4
to provide the wide glass knives that would cut small specimens embedded in
GMA. The knife breaker was capable of cutting quite good knives from 6mm
glass for routine thin sectioning. As far as I am aware, the one I bought
in 1983(?) is still working.

Fred Monson

Frederick C. Monson, PhD
The best research
Center for Advanced Scientific Imaging
occurs before work
West Chester University
at the bench.
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


} ----------
} From: Caroline Miller
} Sent: Monday, January 21, 2002 12:38 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Knifebreakers
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would like information about an older Sorvall glass knife breaker
} making knifes that are 12-13 mm wide. Caroline Miller
}
}
}




From daemon Wed Jan 23 11:50:44 2002



From: Maria Ericsson :      maria_ericsson-at-hms.harvard.edu
Date: Wed, 23 Jan 2002 12:49:05 -0500
Subject: looking for used Leafscan45

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

We're looking for a used Leafscan45 film scanner for our EM Facility.
Any ideas where I could look for one are welcome.

Thanks,

Maria

____________________________

Maria Ericsson
Harvard Medical School EM Facility
220 Longwood Avenue
Boston, MA 02115
(617) 432 1698
maria_ericsson-at-hms.harvard.edu
http://www.hms.harvard.edu/core/em.html


From daemon Wed Jan 23 11:50:44 2002



From: John Olesik :      olesik.2-at-osu.edu
Date: Wed, 23 Jan 2002 12:40:59 -0500
Subject: Electron Microscopist/Microprobe Analyst Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopist and Microprobe Analyst

The Ohio State University Microscopic and chemical Analysis Research Center
(MARC) is searching to fill an immediate opening for an outstanding Electron
Microscopist and Microprobe Analyst (University title: Research Associate
2-Physical). This person will be responsible for operation of scanning
electron microscope (SEM) and electron microprobe (EPMA) instruments. In
addition, the position includes working with researchers, teaching students
the basic theory and practical SEM and EPMA measurement techniques,
collaborating on research projects and directing staff assistants. We seek
an excellent electron microscopist/microprobe analyst who enjoys the
exciting atmosphere of a major research university and wants to collaborate
with scientists on a broad variety of projects. The MARC serves the entire
Ohio State University as well as other institutions, with projects from
diverse research areas including geology, materials science, environmental
sciences, biological and biomedical sciences, dentistry and textiles. The
MARC focuses on elemental analysis using from microscale to bulk analysis
using SEM, EPMA, x-ray fluorescence, inductively coupled plasma optical
emission spectrometry and inductively coupled mass spectrometry with laser
ablation or solution sampling. The MARC also teaches traditional or short
courses on each of the techniques. The position is a full time, hard money
funded job.

Experience with both EPMA and SEM is preferred but strong candidates with
extensive experience using one of these will be seriously considered.
Excellent candidates with a range of experience and potential to grow will
be considered.

If you know of a suitable candidate for this position, please contact Dr.
John Olesik, MARC Director, at {mailto:olesik.2-at-osu.edu} or (614) 292-6954.
Details on official application for the position can be found at The Ohio
State University jobs listing web site ( {http://hr.osu.edu/es/jobsort.htm} ).
Search in the UPP section by title “Research Associate-2 Physical”. The job
listing is number U-20908-012202. Although the official deadline is January
28, 2002 and the listing will be on the OSU jobs list web site for only one
week, the search will continue until an excellent candidate is hired.


----------------------------------------------------------------------------
--
John Olesik
Adj. Assoc. Professor, Research Scientist
MARC Director
Microscopic and chemical Analysis Research Center
Ohio State University
125 S. Oval Mall
275 Mendenhall Laboratory
Columbus, OH 43210

Office: 026D Mendenhall
Phone: (614) 292-6954
FAX: (614) 292-7688
E-mail: olesik.2-at-osu.edu
Web page: www.geology.ohio-state.edu/marc
----------------------------------------------------------------------------
--



From daemon Wed Jan 23 12:14:11 2002



From: Suzanne Adams :      adamss-at-ohsu.edu
Date: Wed, 23 Jan 2002 10:06:38 -0800
Subject: Salary Survey for Electon Microscopy Technologist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Oregon Health & Science University is requesting your help in gathering salary data for Electron Microscopy Technologist.

The Electron Microscopy Technologist prepares human tissue samples for electron microscopy and performs ultrastructural electron microscopic examinations and photographic documentation for the purpose of detecting pathology and guiding medical therapy in support of State, regional, and local clinical and research pathology programs. Employees in this class may instruct researchers, physicians, and medical students in the use of the electron microscope and associated laboratory facilities and equipment. Employees in this class perform routine repair and maintenance of electron microscopes, microtones, light microscopes, evaporators, and other laboratory equipment.

Do you have a job match? Yes_________ No__________

Salary: MIN_______________ MAX____________________

Please feel free to call Suzanne Adams, OHSU Compensation Analyst at (503) 494-3423 or email at adamss-at-ohsu.edu with questions. Thank you in advance for your help with this survey. You may fax the survey back to me at (503) 494-0045.

Thank you.

Suzanne Adams
Compensation Analyst
Oregon Health & Sciences University
Portland, OR
503 494-3423
adamss-at-ohsu.edu



From daemon Wed Jan 23 13:35:43 2002



From: Michael.A.Peters-at-grace.com
Date: Wed, 23 Jan 2002 14:24:31 -0500
Subject: Vector imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a recent article on SEM imaging, a new method/imaging technique(to
me)was mentioned (Vector Imaging). What is Vector imaging and why would
it be used instead of rastering?

Thanks In Advance,
Mike Peters
Davison Chemical



From daemon Wed Jan 23 16:04:09 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 23 Jan 2002 13:56:15 -0800
Subject: Re: looking for used Leafscan45

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Try Professional Marketing Services
Phoenix, AZ
480.940.5400
http://www.promarketinc.com

Their latest listing has one priced at $3995 with
SCSI/GPIB interfaces.

gary g.


At 09:49 AM 1/23/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jan 23 16:04:09 2002



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Wed, 23 Jan 2002 16:43:08 -0500
Subject: Please subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


please subscribe

delannoy-at-jhmi.edu



From daemon Wed Jan 23 21:35:21 2002



From: schlor27-at-aol.com ()
Date: Wed, 23 Jan 2002 21:24:28 -0600
Subject: Ask-A-Microscopist: QC Microscopy work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (schlor27-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
January 23, 2002 at 10:04:59
---------------------------------------------------------------------------

Email: schlor27-at-aol.com
Name: Lori Mead

Organization: FP & M

Education: Undergraduate College

Location: City, State, Country

Question: Hello Listers

I am interested in having some QC Microscopy work done on several
Polymer samples. I would like to hear from labs that are available
to do this type of work.

Regards

Lori


---------------------------------------------------------------------------


From daemon Wed Jan 23 21:35:21 2002



From: usseacat-at-iserv.net ()
Date: Wed, 23 Jan 2002 21:25:44 -0600
Subject: Ask-A-Microscopist:LM repairs or supplies parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (usseacat-at-iserv.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
January 22, 2002 at 23:25:20
---------------------------------------------------------------------------

Email: usseacat-at-iserv.net
Name: Dr. Carl J. Jungblut

Education: Graduate College

Location: Holland, Michigan USA

Question: Firstly, thank you for your help. Ihave two basic questions:
(1) Name and address of a company that repairs or supplies parts for
a monocular, compound Bausch and Lomb microscope.

(2) Name and address(es) of company/companies selling microscope
supplies, especially Wright's stain.

Thank you!

---------------------------------------------------------------------------


From daemon Thu Jan 24 03:59:38 2002



From: Dirk Kirch :      kirch-at-imm.rwth-aachen.de
Date: Thu, 24 Jan 2002 10:50:16 +0100
Subject: SEM, hot stage, detector problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Users,

I have a particular problem concerning in-situ investigations of grain
boundary migration with a Jeol 820 SEM. For that purpose we use
a hot stage to heat up the the specimen up to 600 C. But at a
temperature of about 370 C the thermal radiation of the specimen
and the furnace disturbs the electron signal and we do not see
anything on the screen.

We use a semiconductor BSE-detector with two half- which is
positioned underneath the pole-piece.

I would be nice to hear some suggestions concerning this problem.

Maybe another kind of detector, filters for the photonic radiation
etc.

Further information:

1.We cannot use the SE2-detector since SE cannot solve the
orientation-contrast between the different grains.

2.The temperature at the detector is about 100 C during the
heating process.



Dirk Kirch





+++++++++++++++++++++++++++++++++++++++++

Dirk Kirch
Institut fuer Metallkunde und Metallphysik
RWTH Aachen
D-52056 Aachen
Germany

Phone : +49-241-8026861
Fax : +49-241-8022301
Internet: http://www.imm.rwth-aachen.de
E-Mail : kirch-at-imm.rwth-aachen.de

+++++++++++++++++++++++++++++++++++++++++


From daemon Thu Jan 24 07:40:21 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Thu, 24 Jan 2002 14:32:56 +0100
Subject: Zeiss 3D-Deconvolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,

Is there anyone out there using the 3D-deconvolution program from Zeiss? I
have some 3D images that I created with the LSM510 confocal microscope and
I'm thinking of buying the Zeiss-program to deconvolute, but is it worth
it's price or is there a cheaper/better substitute? Can someone run the
program once on one of my images so I can see the effect?
Thanks,

Sven Terclavers
Molecular Cardiovascular Medicine Group
Louvain - Belgium



From daemon Thu Jan 24 10:06:28 2002



From: Judy Trogadis :      trogadisj-at-smh.toronto.on.ca
Date: Thu, 24 Jan 2002 10:50:52 -0500
Subject: Laser Capture Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists,

We are planning to purchase a laser capture microscopy system and are hoping for input from users of this technology. We really have no experience in this area, therefore, are not even sure what questions to formulate when evaluating a system.

Basically, we are considering the Arcturus PixCell II from Leica - which uses paraffin and frozen sections and the PALM Laser-MicroBeam System from Zeiss which I believe allows capture of cells from living tissue as well.

All comments and suggestions would be greatly appreciated.

Thank you

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON
M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Thu Jan 24 10:23:27 2002



From: Owen P. Mills :      opmills-at-mtu.edu
Date: Thu, 24 Jan 2002 11:19:01 -0500
Subject: wanted: Link EDS hardware

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm looking for Link eXL hardware (beige units - not grey). I need Lemas
(desktop stage control) module, computer boards, etc. Contact me off line
if you have any of this equipment.

Owen

Owen P. Mills
Electron Optics Facility Engineer
Michigan technological University
Materials Science and Engineering
Rm 626 M&M Bldg.
Houghton, MI 49931
906-487-2002 Ph
906-487-2934 Fax
opmills-at-mtu.edu
www.mm.mtu.edu/~opmills/



From daemon Thu Jan 24 10:44:23 2002



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 24 Jan 2002 11:31:26 -0500
Subject: wanted: Link EDS hardware

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I could use spares for the Link eXL grey units.
Thank you.

Please contact:
Jim Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
Storrs, CT 06269-2242
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Jan 24 10:46:28 2002



From: Vickie Frohlich :      frohlich-at-uthscsa.edu
Date: Thu, 24 Jan 2002 10:45:41 -0600
Subject: Optical Microscopy Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The University of Texas Health Science Center at San Antonio (UTHSCSA)

with Support from Hamamatsu Photonics KK

will offer a course on

"Optical Microscopy in the Biological Sciences"

June 5-12, 2002
Application Deadline -March 1, 2002


Tuition - $1,750 (includes room and board) $1300 (without room)
$$$$$ Limited number of complete scholarships are available $$$$$


Topics to be covered:
Microscope Optics: Phase Contrast, Dark-field, DIC, Polarization
Detectors * Digital Processing * Fluorescence Filters and Probes
Live Cell Imaging * FRET * FLIM * Green Fluorescent Proteins
Confocal * Multiphoton * Deconvolution * 3-D Reconstruction

Faculty:
Robert Blystone, Trinity University * Victoria Centonze Frohlich, UTHSCSA
* Robert Hard, SUNY-Buffalo
Brian Herman, UTHSCSA * Ernst Keller, Carl Zeiss * James Lechleiter, UTHSCSA
Kate Luby-Phelps, Medical College of Wisconsin * Masafumi Oshiro, Hamamatsu
Photonics KK
Peter So, MIT * Kenneth Spring, NIH * Simon Watkins, Univ. Pittsburgh

Participating Vendors:
Bio-Rad Inc., Carl Zeiss, Inc., Chroma Technology Corp., Coherent Laser
Group, Compix Inc., DVC Co. Inc., Hamamatsu Photonics Systems, Improvision
Inc., Intelligent Imaging Innovations, Leica Inc./Meyer Instruments, Inc.,
Media Cybernetics, Molecular Probes, Nikon Inc., Olympus America, Inc.,
Omega Optical, Optronics, Roper Scientific, Universal Imaging Corp.

For admission application form and information visit:

www.uthscsa.edu/csb/image/Announcements.html

or contact

Microscopy Course
Department of Cellular and Structural Biology
UTHSCSA, Mail Code 7762
7703 Floyd Curl Drive
San Antonio, TX 78229-3900




From daemon Thu Jan 24 15:31:25 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 24 Jan 2002 13:24:11 -0800
Subject: Re: SEM, hot stage, detector problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The typical solid state BSE detector is fundamentally
a reverse biased diode. When a BSE hits the diode,
current flows. This current is converted to a voltage
and displayed on the SEM's monitor. A solid state
diode has a characteristic known as leakage current.
This current increases with temperature. Thus, I
suspect that your solid state BSE detector diodes are
becoming saturated via high temperature leakage
current. As a consequence, there is no useable
signal.

The option for using these solid state detectors in
a high temperature environment is to thermally
cool them. This could be done using a Peltier cooler.
However, this would be difficult to set up and would
reduce your working distance.

An alternative, which is not thermally sensitive, is
to use a scintillator BSE detector such as the Robinson
Model 6. While not a low cost or zero cost solution,
I think you would find the Robinson detector to solve
your problem and even provide better performance
than a solid state detector does. You should check
with Robinson to be sure that the temperature at the
BSE probe position does not exceed its specified
or allowed temperature. The scintillator detector
works on a totally different principle. But the detector
probe will still have some upper limit of temperature
based on when it might be damaged by heat. If this
temperature limit is too low for your application, then
of course, the detector would not be a viable option.

It would help to know what the temperature is at the
pole piece based on specimen temperature and working
distance.

Gary Gaugler



At 01:50 AM 1/24/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jan 24 19:34:54 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 25 Jan 2002 14:25:05 GMT+1200
Subject: anti-backstreaming traps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Happy New Year

I've forgotten who makes/sells those regeneratable back-streaming
traps which, I think, are called Micro Maze, and I can't seem to
sucessfully websearch for them.

Can someone point me in the right direction, please?

cheers

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Jan 24 19:34:55 2002



From: Jon Hiller :      hiller-at-anl.gov
Date: Thu, 24 Jan 2002 19:26:21 -0600
Subject: IC package removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anybody have a chemical solution for removing IC packaging without
damaging the internal components? Simple grinding from the top down is not
suitable because I need all the interconnects intact. Any help in this
matter is greatly appreciated.

Sincere regards,

Jon Hiller
==================================================================
Jon M. Hiller
Argonne National Laboratory
Materials Science Division
Electron Microscopy Center
Tel: 630-252-9558
Fax: 630-252-4798
Email: hiller-at-anl.gov
==================================================================


From daemon Thu Jan 24 19:34:57 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Thu, 24 Jan 2002 19:27:07 -0600
Subject: Temperature controlled Stages

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to get the names of some recommended vendors of temperature
controlled stages that I can use to observe crystal formation by optical
microscopy. Stage should have a temperature range of 5 to 60 deg. C. and
should have at least two fluid connections. Any recommendations would be
greatly appreciated. Vendors may contact me directly. TIA

Damian Neuberger, PhD
Senior Research Scientist
Baxter Healthcare Corp
POBox 490, WG3-2S
Round Lake, IL 60073
Fax 847.270.5888


From daemon Thu Jan 24 19:36:40 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 25 Jan 2002 14:29:53 GMT+1200
Subject: Re: anti-backstreaming traps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Of course, about a millisecond after I pressed the "send" button, I
remembered that they are from Lesker

thanks anyway

rtch



} From: Self {GLGNOV2/RSIMS}
} To: Microscopy-at-sparc5.Microscopy.Com
} Subject: anti-backstreaming traps
} Date: Fri, 25 Jan 2002 14:25:05 GMT+1200

} Happy New Year
}
} I've forgotten who makes/sells those regeneratable back-streaming
} traps which, I think, are called Micro Maze, and I can't seem to
} sucessfully websearch for them.
}
} Can someone point me in the right direction, please?
}
} cheers
}
} rtch
}
}
Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Fri Jan 25 05:59:11 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 25 Jan 2002 06:50:07 -0500
Subject: Re: SEM, hot stage, detector problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,
The BSE detectors are also "solar cells" and are quite sensitive to
both visible light and infrared. The latter is most likely what is
wiping out the signal. You're right that a Robinson would probably fis
the problem if it can take the heat.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The typical solid state BSE detector is fundamentally
} a reverse biased diode. When a BSE hits the diode,
} current flows. This current is converted to a voltage
} and displayed on the SEM's monitor. A solid state
} diode has a characteristic known as leakage current.
} This current increases with temperature. Thus, I
} suspect that your solid state BSE detector diodes are
} becoming saturated via high temperature leakage
} current. As a consequence, there is no useable
} signal.
}
} The option for using these solid state detectors in
} a high temperature environment is to thermally
} cool them. This could be done using a Peltier cooler.
} However, this would be difficult to set up and would
} reduce your working distance.
}
} An alternative, which is not thermally sensitive, is
} to use a scintillator BSE detector such as the Robinson
} Model 6. While not a low cost or zero cost solution,
} I think you would find the Robinson detector to solve
} your problem and even provide better performance
} than a solid state detector does. You should check
} with Robinson to be sure that the temperature at the
} BSE probe position does not exceed its specified
} or allowed temperature. The scintillator detector
} works on a totally different principle. But the detector
} probe will still have some upper limit of temperature
} based on when it might be damaged by heat. If this
} temperature limit is too low for your application, then
} of course, the detector would not be a viable option.
}
} It would help to know what the temperature is at the
} pole piece based on specimen temperature and working
} distance.
}
} Gary Gaugler
}
}
}
} At 01:50 AM 1/24/2002, you wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Users,
} }
} } I have a particular problem concerning in-situ investigations of grain
} } boundary migration with a Jeol 820 SEM. For that purpose we use
} } a hot stage to heat up the the specimen up to 600 C. But at a
} } temperature of about 370 C the thermal radiation of the specimen
} } and the furnace disturbs the electron signal and we do not see
} } anything on the screen.
} }
} } We use a semiconductor BSE-detector with two half- which is
} } positioned underneath the pole-piece.
} }
} } I would be nice to hear some suggestions concerning this problem.
} }
} } Maybe another kind of detector, filters for the photonic radiation
} } etc.
} }
} } Further information:
} }
} } 1.We cannot use the SE2-detector since SE cannot solve the
} } orientation-contrast between the different grains.
} }
} } 2.The temperature at the detector is about 100 C during the
} } heating process.
} }
} }
} }
} } Dirk Kirch
} }
} }
} }
} }
} }
} } +++++++++++++++++++++++++++++++++++++++++
} }
} } Dirk Kirch
} } Institut fuer Metallkunde und Metallphysik
} } RWTH Aachen
} } D-52056 Aachen
} } Germany
} }
} } Phone : +49-241-8026861
} } Fax : +49-241-8022301
} } Internet: http://www.imm.rwth-aachen.de
} } E-Mail : kirch-at-imm.rwth-aachen.de
} }
} } +++++++++++++++++++++++++++++++++++++++++
}
}
}
}




From daemon Fri Jan 25 08:01:03 2002



From: Diane.Ciaburri-at-gd-ais.com
Date: Fri, 25 Jan 2002 08:50:58 -0500
Subject: Re: IC package removal (LONG)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jon,

I assume you're speaking about plastic packages since you want a chemical
removal technique. Below your message is a summary of responses that I
received when I asked a similar question. I can't comment on them,
because my project dried up after I asked the question and I never got to
try any of the techniques, but there look like some good ones.

Diane Ciaburri
General Dynamics
Pittsfield, MA





Does anybody have a chemical solution for removing IC packaging without
damaging the internal components? Simple grinding from the top down is
not
suitable because I need all the interconnects intact. Any help in this
matter is greatly appreciated.

Sincere regards,

Jon Hiller
==================================================================
Jon M. Hiller
Argonne National Laboratory
Materials Science Division
Electron Microscopy Center
Tel: 630-252-9558
Fax: 630-252-4798
Email: hiller-at-anl.gov
==================================================================




Here's the summary (long) for all those interested in deencapsulating
plastic
encapsulated ICs. I have no preferences as I haven't tried any yet, but
thought the fuming sulfuric acid might be 'fun'. Thanks again!

_______________________________________________________________________
********************************************************************************
********************

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hi Diane,

The way this is generally done is to mill the plastic down on a grinding
wheel to the point where only a fairly thin layer of plastic remains.
Then,
using a plasma etcher, and a mixture of oxygen to CF4 (for example, 30%
oxygen/70% CF4), whereby the oxygen etches away the plastic and the CF4
etches away the glass frit that is usually found in the plastic, you can
remove the remaining plastic (package) without damaging the device itself.
Different people like to use different gas ratios, of course, and that is
probably a function, at least to some degree, of the concentration of
glass
frit in their particular plastic.

The SPI Plasma Prep II unit, as shown on URL
http://www.2spi.com/catalog/instruments/etchers1.html in the world, is
probably the most widely used unit for doing this type of operation. It
is
inexpensive and highly reliable, and requires virtually no maintenance.

Chuck
____________________________________________________________________________
********************************************************************************
***************************
The ion beam approach works well. I have not used it recently on finer
pitch
ICs. With as-built feature sizes of 2-4u, it is fine. It will stop at
the
passivation and leave the Al bond wires intact. The resulting package
looks
like it has a V-shaped pit in it (which it does). The extent of the pit
depends
on the size of the die and if you want to blast
down to the lead frame or substrate.

I have not done this on finer pitch devices. I would be a bit skeptical
about
these mostly because of the smaller bond pads. The etching would still
stop at
the passivation.

There are numerous places in Silicon Valley that do this on an outsource
basis.
Typical costs are about $75 per IC. I can get some contacts for you if
you'd
like.

gary g.
___________________________________________________________________________
********************************************************************************
*************************

Diane: attached is a text document outlining the procedure my FA lab
uses.
Yellow fuming nitric is usually the acid of choice. If you can get a few
extra
parts to practice on, that would be best. And you're right, plasma takes
FOREVER. If I can be of any more help, please let me know.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Labs--SEM/FIB/FA
Kilby Center West
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


TOOLS, EQUIPMENT, & SUPPLIES
1. Milling machine and appropriate end mills
2. Stabilo SuperFine OH pen or equivalent
3. Fume hood properly equipped for exhausting acid fumes and solvent
vapors (see
reference 3.4)
4. Explosion proof hot plate (see reference 3.4)
5. Ultrasonic cleaning apparatus
6. An optical microscope capable of 100X to 500X magnification, equipped
with a
lighting system.
7. Chemical resistant latex gloves
8. Chemical resistant laboratory coat
9. Chemical resistant safety glasses (see reference 3.5)
10. Hand tools (tweezers, scalpels and etc.)
11. Plastic micro-pipette
12. Fuming red nitric acid
13. Yellow nitric acid
14. Methyl alcohol
15. Acetone


1. RECORDING OF PACKAGE MARKINGS
Record all of the device markings that are on the top and bottom sides of
the
devices prior to starting any of the decap operations.
2. CAVITY MILLING
Determine the exact location of the chip within the package and mark the
top of
the device package showing the chip perimeter, using a Stabilo SuperFine
OH pen
and a straight edge. A SAM plot or X-ray image may
be used to help determine the exact location of the chip and also to
determine
the thickness of the mold compound covering the chip.
Note: This should be done on devices having large chips. Devices with
small
chips (less than 0.125 inches in their longest dimension) do not require
this
step.
Mill a cavity out of the plastic package that is centered over the chip.
The
size of the milled cavity should typically be .050 to .100 inches larger
than
the length and width dimensions of the chip. The depth of the
milled cavity depends on the thickness of the mold compound and the
location and
loop height of the bond wires. During the milling operation use a vacuum
line to
pick up the loose plastic particles generated.
Caution: do not mill into the bond wires or the chip. Mill counter bores
on
devices with chip dimensions greater than 0.400 inches on a side. These
counter
bores should be made on one or more levels within the bond pad perimeter
and at
the outermost corners of the cavity (this is necessary to facilitate
etching of
the mold compound at the corners of the chip before the sides are exposed
and
subsequent damage to the leadframe). Care must be taken during the milling
operation to avoid excessive pressure on the mill resulting in filler
induced
damage to the chip P.O. The end mill should not bind, bend, or "smoke"
during
the milling operation.
3. PACKAGE ETCHING
All etching must be performed in a chemical hood that meets the
requirements
defined in references #.3 and
4.Heating of acid or device prior to application of acid must be done
using an
explosion proof hot plate that meets the requirements of reference #4.
Obtain the appropriate acid for use on the mold compound being removed.
Following are the acids that have been identified for the removal of the
various
mold compounds.
Mold Compound Acid/Temperature
Shinetsu Red fuming nitric acid at 140-150 degrees Celsius
Plascon & Sumitomo Red fuming or yellow nitric acid 140-150 degrees
Celsius
Note: Fuming sulfuric acid reacts with exposed aluminum bond pad
metallization
and may result in ball bond discontinuity thus hampering further analysis.

· When using red fuming nitric acid it may be helpful to start the etching
process using a mixture of red and yellow nitric acids in order to slow
down the
etch process until a "residue crust" is formed over the cavity and then
switch
to the red nitric acid.
· Apply the acid in drops using a plastic micro-pipette. The drops should
be
placed in the center and at the corners of the cavity in approximately a
1:1
ratio.
· Allow the acid to react with the mold compound and form a crust of
dissolved
compound. Caution: Do not allow the crust to dry out completely before
adding
additional drops of acid.
· Remove the dissolved material using cotton swabs or by rinsing with
acetone
when the dissolved materials threaten to spill over the cavity. Caution:
Rinse
the device immediately with acetone if acid
spills onto the package pins.
· Soak the device in acetone for a minimum of 10 minutes, followed by a
spray of
methanol to remove loose residue and to clean the residue from the cavity
rim.
· Perform a thorough microscopic inspection to determine whether all
necessary
areas of the chip are exposed.
· If dried mold compound residue persists on the chip surface, use the
following
in the order shown to attempt removal:
· Solvent bath (such as methyl alcohol) in ultrasonic cleaner
· Several drops of room temperature fuming sulfuric acid applied to the
chip
(with the chip at room temperature) for several seconds then rinse the
device in
DI water.
· Several drops of fuming sulfuric acid applied to the chip with the chip
on a
100 degree Celsius hot plate. Note: Fuming sulfuric acid will attack
aluminum
bond pads and is therefore the method of last resort.

_____________________________________________________________________
*********************************************************************
Can't comment on sulfuric, but I have used red fuming nitric at near
boiling
temperature. Apply acid, let react. Flush witn more acid, let react,
etc.

Woody White

_______________________________________________________________________
***********************************************************************

Diane,
Yes, hot fuming sulfuric and/or hot fuming nitric are used as standard
procedures for removing plastic from IC's. The process is not quite that
simple. For example, water rinses will almost certainly etch the bond
pads
on the IC and thus removing connection to the outside world. Additionally,
the plastic contains fire retardants which some regions don't like being
washed down the drain. There is more detailed help through EDFAS.org (one
of ASM's branches). B&G International sells a very safe, effective etcher
which performs decapsulation automatically in minutes.

I have no association with B&G International.

David Saxon
Analytical Microscope Services
11826 Reservoir Rd. E.
Puyallup, WA 98374
253-848-7701 voice & fax
email: info-at-analyticalmicroscope.com
website: www.analyticalmicroscope.com

_________________________________________________________________________
*************************************************************************
Diane,

I used to do failure analysis on semi conductor memories which
were
starting to be made of plastic/epoxy with glass rods about 20 years ago.

I have some technics and possible help but its too much to write.
Basically you drill a small hole about 0.1" deep then heat the IC on a hot
plate and then you drop your acid to remove the plastic. I dont know
chemistry, I'm and Electronics Technician. I did this work with a meterial
sicentist, my mentor.
We used fumming sulfuric acid and fuimg nitric acid, also some type of
organic pink and blue solutions to stop some of the acids etching effect.

The company back then was Burroughs Corp. today is Unysis.

I presently work for the U S Department of Energy in New York City. My
phone number is 212 6203650, I'll be happy to walk through some ideas and
things I learned.

Regards
Peter Roiz
______________________________________________________________________
**********************************************************************

Perhaps it's time to comment on this thread.

Dichloromethane and dimethylformamide are relatively effective disrupters
of
most epoxies but their action is accompanied by great swelling because the
polymer becomes engorged with the liquid before any significant solvation
takes
place. This will destroy wire bonds on an IC.

Fuming (essentially anhydrous) sulfuric acid acts by the completely
different
process of sulfonating reactive groups that remain on the polymer. The
depolymerized and sulfonated byproducts are quite soluble not only in the
acid
but usually in water as well. The worst thing that you could do in this
relatively straightforward process is to wash with water at intervals
because
this would initiate almost instantaneous corrosion. It would be advisable
for a
chemist, as someone trained in the handling
of reactive materials, to carry this out or at least to establish
procedures and
train others with less experience. The action of sulfuric acid in this
regard
is quite different than that of nitric. Nearly anhydrous nitric acid
(completely anhydrous is extremely difficult to prepare) is a very
powerful
oxidizer and could lead to unstable, dangerous byproducts whereas the
sulfonates
resulting from the sulfuric acid reaction are relatively stable. Water
must, of
course, be prevented from splashing into any concentrated acid, especially
sulfuric.

A very strong acid such as sulfuric behaves completely differently in the
absence of water. Since most acids are highly hygroscopic and are sold as
water
solutions, most people do not observe this other side of their behavior.
Without water to create an ionized electrolyte, corrosion of metals will
not
take place. I have de-encapsulated ICs for failure analysis in 200 degree
sulfuric acid and been able to operate the IC
without replacing the .001" aluminum wirebonds that it came with. I
recall one
instance where our company built prototype hybrid microelectronic circuits
out
of such de-encapsulated ICs when their supplier was late getting a new
design on
the market and the only ones available were already encapsulated.

The key is to realize that water must be excluded until the sulfonating
acid has
been completely rinsed away by a non-aqueous liquid. As Mr. Saxon said,
there
are simple and safe devices available for doing this operation. However,
with
proper care and protective gear it can be done in a beaker on a hot plate
in a
fume hood. A few ml.s of sulfuric acid are heated to drive off water
until
heavy vapors are observed over the liquid (which may darken during heating
due
to trace impurities). The IC is carefully lowered into the hot acid and a
vigorous reaction ensues with the epoxy
almost instantly washing into the solution. After a few seconds the IC is
then
quickly lifted out and held over a receiving vessel and flooded with a
stream of
ethanol. Only after this is a final rinse in deionized water carried out,
followed by fresh electronic grade ethanol and forced drying in warm air.

The ready made devices which carry out the operation are typically a small
bowl
with a hinged lid from which air is withdrawn by a gentle vacuum. An
inert
metal feeder tube leads from a heated reservoir for the sulfuric acid and
passes
through the wall of the bowl to a position where the encapsulated device
is
secured. When the lid is closed and the slight vacuum applied, the hot
acid is
pulled into the bowl over the device. It is somewhat self-limiting in
that, if
the lid is opened, there is no driving force to bring more acid into the
container. Naturally, the vacuum source needs to be protected by a trap
and all
waste products properly handled no matter how the procedure is carried
out.

John Twilley
Conservation Scientist
(formerly, Manager of the Reliability Analysis Center,
Teledyne Microelectronics)



From daemon Fri Jan 25 08:35:53 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 25 Jan 2002 08:35:29 -0600
Subject: Hitachi S-570 vibration dampers

Contents Retrieved from Microscopy Listserver Archives
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Listers,

There were some posts recently from folks looking to pass on Hitachi
S-570 SEMs. I'm in a different boat, keeping ours running. Happily,
it's doing well. We do need to replace the vibration dampers just
under the column (at the corners). New ones from Hitachi are horribly
expensive, of course. There is a good home-brew damper our service
engineer described, but I thought I'd check to see if anyone who had
disposed of a 570, or otherwise has extra parts, happened to have
these dampers. If so, how much would you want for them?

Thanks!

Phil
--
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Jan 25 11:00:08 2002



From: Jon Mulholland :      jwm-at-genome.stanford.edu
Date: Fri, 25 Jan 2002 08:52:01 -0800 (PST)
Subject: EM RA Position availible

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopy Research Assistant

The Cell Sciences Imaging Facility (CSIF) at Stanford University has an
opening for a full- time, EM research assistant. The position requires a
person with extensive and varied experienced in EM techniques including
immunohistochemistry and immunogold. Experience in cryo-specimen
preparation and cryo-ultramicrotomy is also desired. The research
assistant will conduct EM experiments for users (primarily Stanford
researchers) of the CSIF as well as train users on facility electron
microscopes and ancillary equipment. In addition, the research assistant
will help setup and manage the daily operation of the EM core including
ordering supplies and assuring that the EM core is in compliance with all
health and safety regulations. The position requires BA/BS degree in
Biology or related field and a minimum of 3-5 years experience in an
electron microscopy research laboratory. Position requires the ability
and desire to develop and apply new techniques. Some experience and
comfort working in a heterogeneous computing environment (PC, MAC, UNIX)
required. The EM core of the CSIF is a new, full service user facility
with state-of-the-art equipment including a JEOL1230 TEM equipped with a
Gatan 791 ccd camera, Leica Ultracut UCT microtomes and Pelco processing
microwave. The CSIF provides Stanford University researchers with access
to state-of-the-art, cutting-edge instrumentation and methodologies for
confocal, multiphoton, deconvolution and electron microscopy imaging.
The successful applicant will have an opportunity to work on many
different research projects and to learn and apply new techniques.
Stanford University provides excellent benefits and an informal work
environment. Salary commensurate with experience. Interested candidates
should e-mail or fax their resume and letter of application directly to:

Jon Mulholland, Director
Cell Sciences Imaging Facility
Beckman Center B062
Stanford University School of Medicine
Stanford, CA 94305-5301

jwm-at-genome.stanford.edu
650-725-4951 (fax)



From daemon Fri Jan 25 11:00:08 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 25 Jan 2002 11:45:46 -0500
Subject: IC package removal

Contents Retrieved from Microscopy Listserver Archives
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Jon,

If it is the black "plastic" (glass filled epoxy), nitric acid works on
some, and sulfuric
acid works on most. There are also some chemicals called "Dynasolve", that
we
use various types of. The differences are in the speed with which the
material is
removed, and what damage is done to interconnects, etc. If you watch the
time
you have the sample in sulfuric acid, it works for most parts.

Hope this helped.
Regards,
Darrell

Jon Hiller {hiller-at-anl.gov} on 01/24/2002 08:26:21 PM

To: Microscopy-at-sparc5.microscopy.com
cc:



Does anybody have a chemical solution for removing IC packaging without
damaging the internal components? Simple grinding from the top down is not
suitable because I need all the interconnects intact. Any help in this
matter is greatly appreciated.

Sincere regards,

Jon Hiller
==================================================================
Jon M. Hiller
Argonne National Laboratory
Materials Science Division
Electron Microscopy Center
Tel: 630-252-9558
Fax: 630-252-4798
Email: hiller-at-anl.gov
==================================================================






From daemon Fri Jan 25 13:14:43 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 25 Jan 2002 11:06:36 -0800 (PST)
Subject: E3 electroscan ESEM - stage drive motor ?

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,
We have an E3 electronscan ESEM in our laboratory and the most used motor
for the Z-axis has failed. We did a fix by replacing it with the rotation
motor, but we now can't rotate our sample. Does anyone have
any suggestions how we can get an economical replacement for the motor?

I contacted FEI and they want too much money for a replacement - something
like $1300 or more. I also contacted the manufacturer - pittman. They no
longer carry the motor, but will manufacture 25 of them mininum for $300
each.

I was wondering if anyone had a spare replacement motor for this
instrument they can sell or donate? I was also wondering if I could use a
replacement motor from Pittman and build it into the drive myself, using
another encoder and gearbox? The motor has on it WDG#4, 4-phase, 500 cpr,
24:1 G/R, manufactured in 6-18-92.

The pittman web page at:
http://www.pittmannet.com/
has a selection of motors, and I've ordered their catalogue and select
guide, but I have no experience in matching motors (torque/speed/etc).
Anyone with prior experience or helpful advice, suggestions will be
greatly appreciated.

Thank you for your help.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Jan 25 13:54:04 2002



From: Cavin Mooers :      cavinm-at-vsl.cua.edu
Date: Fri, 25 Jan 2002 13:45:40 -0600
Subject: EM 300 AVAILABLE

Contents Retrieved from Microscopy Listserver Archives
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I have an Phillips EM300 TEM free to anyone who wants to come pick it
up. It has multiple stages, EDS detector, (no pulse processor,)and a
port for a camera. It does have a mercury diffusion pump, (a problem
for some.)

If we cannot find a home for it in the next 4-6 weeks, it will be
disposed of. Hopefully, it will not come to that.

Sincerly,

Cavin Mooers, Research Assistant
Vitreous State Laboratory
The Catholic University of America
Hannan Hall
Washington, DC 20064
(202) 319-5346phone
(202) 319-4469fax


From daemon Fri Jan 25 14:04:30 2002



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 25 Jan 2002 15:12:22 -0500
Subject: RE: Micromaze Traps

Contents Retrieved from Microscopy Listserver Archives
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The Micromaze foreline traps are available from the Kurt J. Lesker
Co. of Clariton, Pa., (FAX: 412-233-4275). You can probably reach
them on the internet too, but I don't have an e-mail address readily
at hand. The traps are described, and their use is discussed, on p.
147 of my book 'Vacuum Methods in Electron Microscopy" (for a
description see http://www.2spi.com/catalog/books/book48.html or
http://pup.princeton.edu/titles/6484.html)
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Fri Jan 25 17:38:19 2002



From: ever4us-at-comcast.com ()
Date: Fri, 25 Jan 2002 17:28:10 -0600
Subject: Ask-A-Microscopist:Help LM Info for a Middle School

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ever4us-at-comcast.com ) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
January 25, 2002 at 16:20:49
---------------------------------------------------------------------------

Email: ever4us-at-comcast.com
Name: Denise Everett

Organization: Pitman Middle School

Education: 6-8th Grade Middle School

Location: Pitman, NJ 08071

Question: I've recently become the science coordinator of our school
but my science background is in enzymology so I don't have alot of
experience with microscopy. We are looking to buy some new scopes
and in the process I've been looking at our old ones. The 400x
magnification is pretty unusable on these. Is that supposed to be oil
immersion use only?
These lenses are pretty dirty and have probably not been maintained.
The top objective does not come out for cleaning as far as I can see.
Do I just need to send these to a technician?

---------------------------------------------------------------------------


From daemon Fri Jan 25 18:48:02 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 25 Jan 2002 16:37:16 -0800
Subject: Re: Hitachi S-570 vibration dampers

Contents Retrieved from Microscopy Listserver Archives
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Dear Phil,
The secret that the Canadian rep told me was to put some of the thick
washers that came with the S-570 shipping kit over the rubber vibration
dampers, so that the table is raised up off the shipping posts. You get a
little extra life out of them that way. You will know when the table hits
the posts, since your vibration gets worse.
The vibration dampers for the S-570 are only about one quarter the price of
the ones for the S-2300, so it could be worse.
At 08:35 AM 1/25/02 -0600, you wrote:
} Listers,
}
} There were some posts recently from folks looking to pass on Hitachi
} S-570 SEMs. I'm in a different boat, keeping ours running. Happily,
} it's doing well. We do need to replace the vibration dampers just
} under the column (at the corners). New ones from Hitachi are horribly
} expensive, of course. There is a good home-brew damper our service
} engineer described, but I thought I'd check to see if anyone who had
} disposed of a 570, or otherwise has extra parts, happened to have
} these dampers. If so, how much would you want for them?
}
} Thanks!
}
} Phil
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Sat Jan 26 13:16:35 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 26 Jan 2002 10:07:03 -0800
Subject: Re: Ask-A-Microscopist:Help LM Info for a Middle School

Contents Retrieved from Microscopy Listserver Archives
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} Email: ever4us-at-comcast.com
} Name: Denise Everett
}
} Organization: Pitman Middle School
}
} Education: 6-8th Grade Middle School
}
} Location: Pitman, NJ 08071
}
} Question: I've recently become the science coordinator of our school
} but my science background is in enzymology so I don't have alot of
} experience with microscopy. We are looking to buy some new scopes
} and in the process I've been looking at our old ones. The 400x
} magnification is pretty unusable on these. Is that supposed to be oil
} immersion use only?
} These lenses are pretty dirty and have probably not been maintained.
} The top objective does not come out for cleaning as far as I can see.
} Do I just need to send these to a technician?
}
} ---------------------------------------------------------------------------
Denise -

I'm glad that you've asked for help; we're here to provide it.
There are several topics here. First, new microscopes. Let's assume that
the scopes that you have are salvageable. Since you're in a middle school,
PLEASE consider purchasing "dissecting" rather than compound scopes like
the ones that you have. A lot of introductory microscopy for your age
group is observstion of thick specimens at lower magnifications; looking at
large insects, flowers, shells, etc. So having a mix of types will greatly
expand your capabilities. You'll find a detailed discussion of selection
criteria on Project MICRO's website (URL below). I suggest 20x monocular
dissecting scopes, wich will cost you around $75 each; sources are listed
on the MICRO site and you can find an example online at
www.microscopeworld.com.
Your dirt diagnosis is probably accurate. It would be best if you
learn to clean the scopes yourself; you'll then know how to keep them that
way. The New York Microscopical Society has members and meeting rooms in
New Jersey, and one of their members may be available to show you what to
do; I'm copying this Email to Jean Portell {JeanDP-at-aol.com} , one of their
officers. I also can provide you with detailed cleaning instructions for
teachers, written by a MSA member. 400x is "high dry" - oil immersion is
1000x and inappropriate for middle school.
While you're visiting the MICRO website, don't miss MSA's middle
school manual, "Microscopic Explorations"; it's an excellent introduction
to scientific observation and inquiry, written by the science educators at
the Lawrence Hall of Science. If you want a reference book for your own
use, here's another listing from the MICRO bibliography:

Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy; it's unequalled as a
basic reference for beginners. Almost half of the book is devoted to
simple preparation methods for biological specimens and descriptions (with
good illustrations) of commonly encountered organisms. Adult. RECOMMENDED

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun Jan 27 11:35:11 2002



From: Yann Bassaglia :      bassaglia-at-univ-paris12.fr
Date: Sun, 27 Jan 2002 18:22:19 +0100
Subject: Trouble in LM/EM immunolabbeling correlation

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Dear lister,

I'm new in this list , and I wonder if anybody could help us...

We try to localize an epitope in the sarcomeric organization of rat muscle.
We obtained a very clean and strong signal by immunofluorescence on
cryostat sections, even after fixation with 4%PF. So we planned to go to EM
level, to see if our epitope is in or around the myofibrilles...
But at this time, we were unable to obtain any good signal in EM :
- using post-embedding methods after Lowicryl or LRwhite (and of course
Epon...)
- and even using ultracryotomy...
In this last case, we were able to clearly observe our signal on semithin
sections with immunofluorescence, but no specific signal was obtained on
ultrathin sections with colloidal-gold !

The only result we could get in EM was by pre-embedding, using peroxydase
on cryostat sections. But this is not very clean, and do not allow us to
answer to our question because the precipitate could deposit into the
sarcomere.

I plan to use, in pre or post embedding methods :
- antifluorescein antibodies, linked to gold : I would appreciate any
advice or recommandation about this kind of antibodies (specificity ? can
we hope an amplification ?)
- tyramide-biotin amplification : here also, I would appreciate any advice.
In particular, is there an estimation of the maximum distance between the
site of production and the site of fixation of the tyramide product ?

Any advice, or suggestion for another method, will be wellcome...

Thank you for your help !
Yann

_____________________________________________
Dr. Yann BASSAGLIA, PhD
Universite Paris-Val de Marne
Laboratoire CRRET / UPRESA CNRS 7053
Avenue du general de Gaulle
F-94010 CRETEIL Cedex
FRANCE

Tel : (33) 1 45 17 14 55
Fax : (33) 1 45 17 18 16
e-mail : bassaglia-at-univ-paris12.fr





From daemon Mon Jan 28 07:02:50 2002



From: DrJohnRuss-at-aol.com
Date: Mon, 28 Jan 2002 07:47:00 EST
Subject: Announce: Image Processing and Measurement Workshop

Contents Retrieved from Microscopy Listserver Archives
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The three-day intensive hands-on workshop on Image Processing and Measurement
presented by John Russ (author of "The Image Processing Handbook" and
"Practical Stereology") through the North Carolina State University
Department of Continuing and Professional Education is now in its 20th year.
The course dates for 2002 are May 8 - 10 in Raleigh, NC, June 10-12 at the
Danish Technological Institute in Taastrup, Denmark (near Copenhagen), and
November 6-8, 2002, in Raleigh. This course has generated highly favorable
reviews from the thousands of previous students. The primary focus is on
images from various types of microscopy, with practical guidance in
correcting imaging defects, enhancing the images for presentation and
measurement, and performing stereological meaningful measurements on them.
Textbooks and computer software are provided to attendees. Lab sessions with
an opportunity to bring your own images makes this course immediately useful
and highly productive.

For full information on the course, including outlines, faculty information,
a downloadable brochure, and on-line registration, go to

http://members.AOL.com/IPCourse/

Class size is limited to maintain a high ratio of instructors to students, so
make your reservation now. You may also contact Cindy Allen at NCSU Contin.
Ed., at 919-515-8171


From daemon Mon Jan 28 07:04:55 2002



From: R. Cross :      r.cross-at-ru.ac.za
Date: Mon, 28 Jan 2002 14:59:21 +0200
Subject: ICEM-15

Contents Retrieved from Microscopy Listserver Archives
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News update!

15th International Congress on EM (ICEM-15), Durban, South
Africa, 1 - 6 September 2002.

* abstracts
* scientific programme
* good news for international delegates
* important travel information

As the deadline for receipt of abstracts is rapidly approaching
anyone intending to contribute should send their abstracts as soon
as possible, according to the instructions in the Second Circular
and Call for Abstracts, by courier please to the ICEM-15 office at
this address:

ICEM-15
Turners Conferences
37 Jonsson Lane (off Victoria Embankment)
Durban, South Africa

Those who received the Second Circular will have seen that a wide-
ranging programme of scientific symposia has been put together by
the programme committee, the advisory committee and the IFSEM
executive. This has been further developed and there is now
something for everyone in the programme, and a most impressive
group of scientists from throughout the world has been assembled
to chair the symposia and give invited lectures. This will be
supplemented by the Technical Forum during which issues of a
more technical nature will be discussed.

Excellent news for international delegates is that because of the
complexities of international currency markets the South African
Rand has depreciated to a large extent against the US$ and major
European currencies over the past year. This means that cost of
practically everything such as accommodation, meals, souvenirs,
tours, car hire, etc, for delegates will be very low, and represents
probably the best value for money that is obtainable anywhere.

Because the 2002 Earth Summit is taking place in Johannesburg
during August, seats on flights to and from South Africa, and to
come extent within Southern Africa, will be in high demand during
that time. Delegates to ICEM-15 are therefore advised to book
early. Contact your travel agent, or email Dudley Randall at Turners
Conferences (turner17-at-galileosa.co.za) for advice and assistance.

We looking forward to seeing as many of you as possible in
Durban in September. Everything is in place to ensure that ICEM-
15 provides all those who attend with a scientifically rewarding,
memorable and most enjoyable experience.

Anyone requiring information should address enquiries to:

icem-at-ru.ac.za (general)
turner17-at-galileosa.co.za (travel, accommodation, etc)
graham-at-nu.ac.za (local arrangements)
bruton-at-nu.ac.za (trade exhibition)

or go to the ICEM-15 websites:

www.icem15.com
www.turners.co.za/icem15

Kind regards.

Robin H Cross
Chairman: ICEM-15






From daemon Mon Jan 28 08:30:33 2002



From: Christopher Ware :      Warec-at-nu.ac.za
Date: Mon, 28 Jan 2002 08:20:06 -0600
Subject: TEM on clays

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Content-Type: text/html; charset=ISO-8859-1
Content-Description: HTML

I am currently looking at TEM of clays within heavy mineral
containing coastal dunes in South Africa. I have completed extensive
X-ray analyses of these clays but the expertise are not available in
our university for the TEM studies. Are there any outstanding books
available on this subject. Any advice would be welcome. Thanks.
Chris Ware
School of geological & computer sciences
University of Natal
South Africa
{mailto:warec-at-nu.ac.za} warec-at-nu.ac.za


From daemon Mon Jan 28 13:25:28 2002



From: Jon Mulholland :      jwm-at-genome.stanford.edu
Date: Mon, 28 Jan 2002 11:14:28 -0800 (PST)
Subject: TEM film processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists,
We are looking into purchasing a processor (Mohr, #2950 from ted
pella) to process our TEM negatives. This processor will be dedicated to
TEM film processing. We would like to hear from others who have used this
type of mechanical processing for TEM negatives (kodak 4489). Does it
leave spots or residues, do you routinely lose negatives, is it expensive
it terms of chemicals etc.? Thanks in advance for your responses.


Jon Mulholland
Cell Sciences Imaging Facility
Beckman Center B050
Stanford University School of Medicine
Stanford, CA 94305



From daemon Mon Jan 28 14:09:57 2002



From: Matt Olszta :      molsz-at-mse.ufl.edu
Date: Mon, 28 Jan 2002 15:03:16 -0500
Subject: TEM Bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am currently trying to embed mineralized (CaCO3) collagen samples in TAAB
epon resin, but am finding that it is not hard enough. As I am not as
familiar with the different resins, I was hoping someone on here would be
able to help me. I heard that I might possibly use PMMA. Any suggestions
would be appreciated.

Regards,
Matt Olszta
Graduate Research Assistant
University of Florida



From daemon Mon Jan 28 15:32:41 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 28 Jan 2002 13:24:07 -0800 (PST)
Subject: Re: TEM on clays

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The journal,
Clays and Clay Minerals, has a lot of articles on clays and often analysis
by TEM. You should do a search of this journal for the particular
clay/minerals you are interested in.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Mon, 28 Jan 2002, Christopher Ware wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Content-Type: text/html; charset=ISO-8859-1
} Content-Description: HTML
}
} I am currently looking at TEM of clays within heavy mineral
} containing coastal dunes in South Africa. I have completed extensive
} X-ray analyses of these clays but the expertise are not available in
} our university for the TEM studies. Are there any outstanding books
} available on this subject. Any advice would be welcome. Thanks.
} Chris Ware
} School of geological & computer sciences
} University of Natal
} South Africa
} {mailto:warec-at-nu.ac.za} warec-at-nu.ac.za
}



From daemon Mon Jan 28 15:33:57 2002



From: Curtis Olson :      COlson-at-scpglobal.com
Date: Mon, 28 Jan 2002 14:26:05 -0700
Subject: SEM sample prep of Semiconductor Xsect

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am new to the SEM field and am tasked with learning how to prepare cross sections of microchips from silicon wafers (features of {0.25um). I have been unable to locate any information so references and any other advice would greatly help. Tools, accessories, books, on-line info., etc. Thank you

Curtis



From daemon Mon Jan 28 17:45:42 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 28 Jan 2002 18:22:05 -0500
Subject: SEM sample prep of Semiconductor Xsect

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1) Get your hands on the MRS books on TEM sample prep III and IV. Sorry, I don't' have the MRS vol numbers on hand, but you can probably look them up on the MRS web site.
2) Look into taking the Lehigh course on TEM sample prep this summer. Two excellent instructors! http://www.lehigh.edu/~inmatsci/shortcourses/Microscourses.html

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Curtis Olson [mailto:COlson-at-scpglobal.com]
Sent: Monday, January 28, 2002 4:26 PM
To: Microscopy-at-sparc5.microscopy.com


I am new to the SEM field and am tasked with learning how to prepare cross sections of microchips from silicon wafers (features of {0.25um). I have been unable to locate any information so references and any other advice would greatly help. Tools, accessories, books, on-line info., etc. Thank you

Curtis



From daemon Mon Jan 28 18:32:30 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 29 Jan 2002 13:24:28 GMT+1200
Subject: Filter funnel for LN2 for EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, listers

When I cleaned out my EDS detector dewar, I discovered quite a bit of
crud, including an ant, plus, of course a few ml of water.

I guess I should try to filter the LN2 as I pour it into the
detector, but I've never been able to visualise what seems like a
satisfactory funnel/filter configuration.

While it would be nice to filter out particles, and maybe even
suspended ice crystals, I don't want to introduce additional
problems.

Neither do I want to go through the occasionally heart-stopping
performance of warming up the detector, taking it off the 840,
cleaning out the dewar, remounting and recooling it any more
frequently than I absolutely have to.

How do others deal with this?


cheers

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Jan 28 21:47:30 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Mon, 28 Jan 2002 22:38:48 -0500 (EST)
Subject: Cacodylate vs. phosphate buffers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all...

Sorry for this really basic question:

Does anyone have any direct experience with using cacodylate vs. phosphate
buffers with glutaraldehyde for fixing spermatozoa? I have a colleague
having some sperm shipped from Africa, and she would like to use
phosphate rather than cacodylate buffer. Also, I'm just curious as to why
one might be superior to the other, barring toxicity issues. I've
always used cacodylate for sperm.

Best,

Angela

Angela V. Klaus, Ph.D.

Director, Core Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977




From daemon Mon Jan 28 21:49:39 2002



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Mon, 28 Jan 2002 19:44:22 -0800
Subject: LM Unitron Objective ID?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Perhaps someone will be able to provide some information regarding an
unusual Unitron microscope objective.

The most unusual aspect of the objective is its length (8 cm or 3 inches).
The threads are standard. It is engraved on one side of the barrel with
"Phase Contrast D. M. UNITRON No.866" I know this indicates a Dark Medium
Phase Contrast objective, and indeed I can see the phase annulus when I
peer through it. But it is several times longer and heavier than another
Unitron Phase objective I have. Althogh I can thread it into the nosepiece
of my Unitron inverted scope, the nosepiece cannot be lowered far enough to
accommodate the objective between the nosepiece and the stage.

The other side of the barrel is engraved "Coated F. F. 40X n.A.
0.45 T.L. 170 Quartz 1.00"

I know that T.L. = Tube Length and N. A. = Numerical Aperture.

What does the F. F. designation mean (Flat Field?) and what is the
significance of the "Quartz 1.00?

What is the application for this objective?
Mike Dalbey

Lecturer in Biology
University of California, Santa Cruz

831-459-3674

dalbey-at-biology.ucsc.edu



From daemon Tue Jan 29 00:45:20 2002



From: Arthur Day :      ard-at-ansto.gov.au
Date: Tue, 29 Jan 2002 17:37:13 +1100
Subject: Re: Filter funnel for LN2 for EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} When I cleaned out my EDS detector dewar, I discovered quite a bit of
} crud, including an ant, plus, of course a few ml of water.
}

Hey rtch,

Did the ant wake up again? Was it thirsty? Just wondering!

Can't really suggest a good filter but the Ln2 shoud be pretty clean
and ice free already? I imagine most filters would make the filling
process a bit of an ordeal. We just make sure the transfer dewars are
completely clean and dry before starting and only carry the Ln2 with
lids on the dewars. Using a lid substantially stops the ice you get
from humidity being pulled out of the air. You should normally be
able to go for many years without the ice getting so bad that it
impacts on the performance of the dewar/eds?

This question has stimulated my curiosity about how long people
generally find they can go before having to de-ice their systems --
in general.

Cheers mate.

Arthur.





Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/


From daemon Tue Jan 29 01:08:53 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Mon, 28 Jan 2002 22:58:57 -0800
Subject: Re: SEM sample prep of Semiconductor Xsect

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Curtis:

You certainly came to the right place. I'm sure there are many members of the list who can share their experience with you on this subject. I would also suggest that you visit our website at www.southbaytech.com. If you click on "Applications Support" you will find sections containing application
notes and technical reports. You will find numerous references to SEM and TEM cross sectioning. You can also type in the keyword "EM" to find all of our EM preparation equipment. Of particular interest will be our Model 590S Tripod Polisher.

In addition to the information you fins on our website, we also have our technical support staff who can walk you through the process. We can offer on-site training classes.

I hope this information helps. If you need anything else, please feel free to contact me directly off-line.

DISCLAIMER: South Bay Technology produces equipment and supplies as described above and, therefore, has a vested interest in promoting their use.

Best regards-

David

Curtis Olson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am new to the SEM field and am tasked with learning how to prepare cross sections of microchips from silicon wafers (features of {0.25um). I have been unable to locate any information so references and any other advice would greatly help. Tools, accessories, books, on-line info., etc. Thank you
}
} Curtis

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Tue Jan 29 01:34:28 2002



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Tue, 29 Jan 2002 02:31:53 -0500
Subject: Re: Contact info for Scanning Microscopy International:

Contents Retrieved from Microscopy Listserver Archives
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Hello Microscopists:

Thanks for your suggestions for contacting Scanning Microscopy
International. This organization closed its Chicago office due to lack of
funds; it is not associated with journal 'Scanning' in any way. No-one was
able to provide current contact information for Dr. Om. Johari.

I was able to obtain the required permission by contacting Dr. Godfried
Roomans, one of the editors of the volume in question, directly, so this
seems to be the approach that works. He's on the MSA membership directory:

Godfried M. Roomans
Medical Cell Biology
Univ Uppsala Box 571
Uppsala, S-75123, SWEDEN
Tel: (46)18 4714114
Fax: (46)18 551120

Rick Powell


*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Tue Jan 29 02:16:43 2002



From: Gerhard Frank :      Gerhard.Frank-at-ww.uni-erlangen.de
Date: Tue, 29 Jan 2002 09:09:05 +0100
Subject: Re: Filter funnel for LN2 for EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Ritchie,
We use a cut 1.5 l PET lemonade bottle (the ones with rather thick walls, no the
thin stuff; it does not get too cold and does not crack if dropped on the floor)
as a funnel with a coffee filter bag taped into it. It works quite well. Be sure
that there is enough space for the nitrogen to escape which boils off during
filling . I always find a lot of debris in the filter when I change it (app. 2
times a year).
Hope this helps
Gerhard Frank


Ritchie Sims schrieb:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, listers
}
} When I cleaned out my EDS detector dewar, I discovered quite a bit of
} crud, including an ant, plus, of course a few ml of water.
}
} I guess I should try to filter the LN2 as I pour it into the
} detector, but I've never been able to visualise what seems like a
} satisfactory funnel/filter configuration.
}
} While it would be nice to filter out particles, and maybe even
} suspended ice crystals, I don't want to introduce additional
} problems.
}
} Neither do I want to go through the occasionally heart-stopping
} performance of warming up the detector, taking it off the 840,
} cleaning out the dewar, remounting and recooling it any more
} frequently than I absolutely have to.
}
} How do others deal with this?
}
} cheers
}
} rtch
}
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand

--
Gerhard Frank
UNIVERSITAET ERLANGEN-NUERNBERG Phone: +49 9131 85 28606
Institut fuer Werkstoffwissenschaften VII Fax: +49 9131 85 28602
Cauerstr. 6, D-91058 Erlangen, Germany Gerhard.Frank-at-ww.uni.erlangen.de


From daemon Tue Jan 29 02:44:58 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 29 Jan 2002 08:50:50 +0000 (GMT Standard Time)
Subject: Re: Filter funnel for LN2 for EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I tend to agree with Arthur's approach. Use clean, dry N2
buckets and don't let the N2 stand before filling to
prevent ice buildup.

Many years ago I used a steel funnel with a mesh filter in
the bottom to prevent water (ice) and dirt from entering
the detectors but it was so much slower that I began to
think that I was more likely to get water in from the ice
buildup during the prolonged fill, so I gave up.

Don't use plastic funnels that are likely to shatter easily
when cold. Apart from the safety aspect a little plastic
piece in the EDX dewar really can affect the performance
from the continual boiling.

As for regular cleaning of the dewar it is not something I
have ever undertaken. We have cleaned out dewars when they
are removed for service etc. (and also to remove a piece of
plastic funnel) but never just to check the dewar is clean.

Regards to all,
Ron


On Tue, 29 Jan 2002 17:37:13 +1100 Arthur Day
{ard-at-ansto.gov.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } When I cleaned out my EDS detector dewar, I discovered quite a bit of
} } crud, including an ant, plus, of course a few ml of water.
} }
}
} Hey rtch,
}
} Did the ant wake up again? Was it thirsty? Just wondering!
}
} Can't really suggest a good filter but the Ln2 shoud be pretty clean
} and ice free already? I imagine most filters would make the filling
} process a bit of an ordeal. We just make sure the transfer dewars are
} completely clean and dry before starting and only carry the Ln2 with
} lids on the dewars. Using a lid substantially stops the ice you get
} from humidity being pulled out of the air. You should normally be
} able to go for many years without the ice getting so bad that it
} impacts on the performance of the dewar/eds?
}
} This question has stimulated my curiosity about how long people
} generally find they can go before having to de-ice their systems --
} in general.
}
} Cheers mate.
}
} Arthur.
}
}
}
}
}
} Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
} Ansto Materials Division Fax: 61-2-9543-7179
} PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
} Australia www: http://www.ansto.gov.au/
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Jan 29 05:32:17 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 29 Jan 2002 11:23:37 +0000
Subject: CCD camera for fluroescence microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am considering purchase of a digital camera for
publication quality photography of fluorescence images
on a Leica MZFLIII stereomicroscope.
I would be grateful for advice / user recommendations on this

with best wishes

Chris Jeffree
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Tue Jan 29 06:22:46 2002



From: Oparowski, Joseph :      Joseph_Oparowski-at-bose.com
Date: Tue, 29 Jan 2002 07:17:39 -0500
Subject: Filter funnel for LN2 for EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richie,

When we fill our dewar, we use a filter wipe (Kimwipes EX-L) to trap any ice
or dirt. These filter wipes are an elongated strip which can be placed in
the dewar neck to create a cavity approximately 3" deep. The extra length
allows the edges to remain outside the dewar neck so it does not fall in.
As with any filter, the fill time takes a little longer. After filling, we
pull the filter wipe out, let it warm up to room temp, and use it to wipe
any frost off of the dewar cap body. As a worst case, I have seen only
~10ml of water (max.) in the dewar over two years.

Hope this helps.

p.s. To follow the usual disclaimers, I have no affiliation with
Kimberly-Clark.

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313


-----Original Message-----
} From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz]
Sent: Tuesday, January 29, 2002 8:24 AM
To: Microscopy-at-sparc5.microscopy.com


Hi, listers

When I cleaned out my EDS detector dewar, I discovered quite a bit of
crud, including an ant, plus, of course a few ml of water.

I guess I should try to filter the LN2 as I pour it into the
detector, but I've never been able to visualise what seems like a
satisfactory funnel/filter configuration.

While it would be nice to filter out particles, and maybe even
suspended ice crystals, I don't want to introduce additional
problems.

Neither do I want to go through the occasionally heart-stopping
performance of warming up the detector, taking it off the 840,
cleaning out the dewar, remounting and recooling it any more
frequently than I absolutely have to.

How do others deal with this?


cheers

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Tue Jan 29 06:25:06 2002



From: Janos Labar :      labar-at-mfa.kfki.hu
Date: Tue, 29 Jan 2002 13:44:39 +0100
Subject: M&M 2001 Long Beach Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

During registration to the Long Beach conference, I checked the option that
the Proceedings volume should be sent to me by post. It still has not
arrived.

Is there anyone in Europe who already received it by post? Might there be a
problem with my copy?

Thank you in advance. Best regards:

János Lábár





Dr. habil, Janos L. Labar
Scientific Advisor
Research Institute for Technical Physics and Materials Science
H-1121 Budapest, Konkoly-Thege u. 29-33
Postal address: H-1525 Budapest-114, Po Box 49
Tel: (36)(1) 392-26-92
Fax: (36)(1) 275-49-96
Fax/Phone: (36)(1) 392-25-86
home page: www.mfa.kfki.hu/~labar




From daemon Tue Jan 29 07:03:10 2002



From: alan stone :      as-at-astonmet.com
Date: Tue, 29 Jan 2002 06:53:49 -0600
Subject: Re: Filter funnel for LN2 for EDS

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We simply clamp in loosely packed cheesecloth and it works perfectly.




At 01:24 PM 1/29/2002 +0000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Tue Jan 29 07:03:11 2002



From: Steve Chapman :      protrain-at-emcourses.com
Date: Tue, 29 Jan 2002 12:15:19 -0000
Subject: Re: Filter funnel for LN2 for EDS

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Hi

One of my clients in New Zealand uses the following filter technique with
great success.

They pass their LN2 through a pad of cotton wool to filter out ice and other
garbage. The important feature of the cotton is that it is the "clean
fibres" type, not the type which has little bobbles within the fibres.

It works for them so if you can get a cotton that does not flake off when a
liquid is passed through it, then I guess you have the right material for
the task?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com



From daemon Tue Jan 29 09:03:05 2002



From: rcmoretz-at-att.net (by way of Nestor J. Zaluzec)
Date: Tue, 29 Jan 2002 08:52:52 -0600
Subject: Re: Cacodylate vs. phosphate buffers

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Angela:
The main reason for using cacodylate instead of
phosphate is that uranyl salts precipitate in the
presence of phosphate. If one uses phosphate in the
original fixative, then several washes into another
buffer (e.g. through Tris into cacodylate) is required
before finishing. Since I use en bloc staining with
uranyl acetate, I prefer to use cacodylate throughout.
I also think (based only on my own biased sampling) that
I have less uranyl precipitation when staining on grids
with cacodylate vs phosphate buffered tissues.
Roger

--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all...
}
} Sorry for this really basic question:
}
} Does anyone have any direct experience with using cacodylate vs. phosphate
} buffers with glutaraldehyde for fixing spermatozoa? I have a colleague
} having some sperm shipped from Africa, and she would like to use
} phosphate rather than cacodylate buffer. Also, I'm just curious as to why
} one might be superior to the other, barring toxicity issues. I've
} always used cacodylate for sperm.
}
} Best,
}
} Angela
}
} Angela V. Klaus, Ph.D.
}
} Director, Core Imaging Facility
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024 USA
}
} Email: avklaus-at-amnh.org
} Tel: 212-769-5977
}
}
}


From daemon Tue Jan 29 10:11:56 2002



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Tue, 29 Jan 2002 10:41:33 -0500
Subject: re:Cacodylate vs. phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
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Dear Angela,

I too prefer cacodylate buffer. However quite a few labs still use
phosphate buffer, probably for safety reasons. I know that phosphate buffer
can produce a precipitate. In Hayat's book Principles and Techniques of
Electron Microscopy, Biological Applications, Third Edition; there is a
section on buffers which may be helpful.
Good Luck,

Jackie Garfield
Lifecell Corporation
One Millennium Way
Branchburg, NJ 08876
(908) 947-1182
e-mail: jgarfield-at-lifecell.com


From daemon Tue Jan 29 10:26:42 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Tue, 29 Jan 2002 10:06:23 -0600
Subject: Exploring an Idea

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List

I have recently seen a micromanipulator assembly that can be added to a
port on an SEM or FIB to probe samples within the vacuum at high
magnifications (submicron resolutions). I would like the List to think with
me for a bit about possible applications for such a device. The obvious one
that comes to my mind is the electrical probing of devices in
microelectronics, or maybe moving micro-particles around on a sample. If any
of you in material science or biology can think of any possible applications
I would like to hear from you off line.


Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu


From daemon Tue Jan 29 10:39:19 2002



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Tue, 29 Jan 2002 08:27:57 -0800
Subject: Re: Cacodylate vs. phosphate buffers

Contents Retrieved from Microscopy Listserver Archives
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Hi Angela
We always use cacodylate buffer unless we are doing immuno labelling.
Cacodylate does not react with uranyl acetate whereas you get aweful
precipitates if there is any suggestion of phosphate buffer left when
the specimen is put into uranyl acetate either with enblock or
on-grid staining.

So if you are using phosphate buffer, you have to be very careful
with washing. We would normally have a warm water wash to remove the
phosphate before UA staining.
Elaine

} Hi all...
}
} Sorry for this really basic question:
}
} Does anyone have any direct experience with using cacodylate vs. phosphate
} buffers with glutaraldehyde for fixing spermatozoa? I have a colleague
} having some sperm shipped from Africa, and she would like to use
} phosphate rather than cacodylate buffer. Also, I'm just curious as to why
} one might be superior to the other, barring toxicity issues. I've
} always used cacodylate for sperm.
}
} Best,
}
} Angela
}
} Angela V. Klaus, Ph.D.
}
} Director, Core Imaging Facility
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024 USA
}
} Email: avklaus-at-amnh.org
} Tel: 212-769-5977

--
Dr. Elaine Humphrey
Director, Biosciences Electron Microscopy Facility
First Vice President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca
website: www.emlab.ubc.ca


From daemon Tue Jan 29 11:17:29 2002



From: Gary Liechty :      gdliechty-at-alliedhightech.com
Date: Tue, 29 Jan 2002 09:12:38 -0800
Subject: SEM sample prep of Semiconductor Xsect

Contents Retrieved from Microscopy Listserver Archives
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Hello Mr. Olson,

Allied High Tech Products, Inc. offers a complete line of equipment and
consumables for sample preparation including IC cross-sections.

You can visit our website for information: www.alliedhightech.com, contact
me to discuss your needs personally, or you can schedule a visit to Allied's
facility for hands on training and demonstration of the equipment and
polishing supplies that are used. An additional option is to have an Allied
Product Application Specialist in your area come and discuss your
applications.

Sincerely,

Gary Liechty
Manager, Technical Products

{ {...OLE_Obj...} }
2376 E. Pacifica Place
Rancho Dominguez, CA 90220

310-635-2466
310-762-6808 Fax

www.alliedhightech.com



-----Original Message-----
} From: Curtis Olson [mailto:COlson-at-scpglobal.com]
Sent: Monday, January 28, 2002 1:26 PM
To: Microscopy-at-sparc5.microscopy.com


I am new to the SEM field and am tasked with learning how to prepare cross
sections of microchips from silicon wafers (features of {0.25um). I have
been unable to locate any information so references and any other advice
would greatly help. Tools, accessories, books, on-line info., etc. Thank you

Curtis




From daemon Tue Jan 29 11:53:53 2002



From: Tina Schwach :      tschwach-at-mindspring.com
Date: Tue, 29 Jan 2002 11:48:12 -0600
Subject: Cacodylate vs. phosphate buffers

Contents Retrieved from Microscopy Listserver Archives
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I used phosphate buffer for years in fixing bacterial samples for EM.
However, when I started adding ruthenium red to my primary fix to better
stabilize acidic mucopolysaccharides found on bacterial surfaces,
precipitates started forming. So I switched to cacodylate buffer and the
problem went away. I then use osmium in the post-fix step. Now, I am
actually thinking of switching to HEPES buffer (used in tissue culture work)
so I can get away from cacodylate and still use RR. I've fixed sperm with
the same protocol.

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112
----- Original Message -----
} From: Angela Klaus
To: microscopy-at-sparc5.microscopy.com
Sent: Monday, January 28, 2002 9:38 PM


Hi all...

Sorry for this really basic question:

Does anyone have any direct experience with using cacodylate vs. phosphate
buffers with glutaraldehyde for fixing spermatozoa? I have a colleague
having some sperm shipped from Africa, and she would like to use
phosphate rather than cacodylate buffer. Also, I'm just curious as to why
one might be superior to the other, barring toxicity issues. I've
always used cacodylate for sperm.

Best,

Angela

Angela V. Klaus, Ph.D.

Director, Core Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977



From daemon Tue Jan 29 12:46:09 2002



From: akc-at-umich.edu
Date: Tue, 29 Jan 2002 13:44:23 -0500
Subject: Re: Cacodylate vs. phosphate buffers

Contents Retrieved from Microscopy Listserver Archives
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Janos,

I have asked Bill Bailey, the editor of the Proceedings to find out if your
copy was shipped or if there was another problem. Once I find out I will let
you know.

Sorry about the problems.

Bob Price,
Program Chair - M&M 2001 Long Beachs

} From: "Janos Labar" {labar-at-mfa.kfki.hu}
To: "Microscopy ListServer" {Microscopy-at-sparc5.microscopy.com}


Another minor reason involves convenience. In my experience, the
concentrated phosphate buffer stock that was stored in the refrigerator
gradually developed crystals that had to be dissolved before the stock
could be used. This didn't happen with the concentrated cacodylate buffer
stock.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Tuesday, January 29, 2002 8:52 AM -0600 "by way of Nestor J. Zaluzec"
{rcmoretz-at-att.net} wrote:

}
} Angela:
} The main reason for using cacodylate instead of
} phosphate is that uranyl salts precipitate in the
} presence of phosphate. If one uses phosphate in the
} original fixative, then several washes into another
} buffer (e.g. through Tris into cacodylate) is required
} before finishing. Since I use en bloc staining with
} uranyl acetate, I prefer to use cacodylate throughout.
} I also think (based only on my own biased sampling) that
} I have less uranyl precipitation when staining on grids
} with cacodylate vs phosphate buffered tissues.
} Roger
}
} --
} Where the world is only slightly
} less weird than it actually is.


} } Hi all...
} }
} } Sorry for this really basic question:
} }
} } Does anyone have any direct experience with using cacodylate vs.
} } phosphate buffers with glutaraldehyde for fixing spermatozoa? I have
} } a colleague having some sperm shipped from Africa, and she would like
} } to use phosphate rather than cacodylate buffer. Also, I'm just
} } curious as to why one might be superior to the other, barring toxicity
} } issues. I've always used cacodylate for sperm.
} }
} } Best,
} }
} } Angela
} }
} } Angela V. Klaus, Ph.D.
} }
} } Director, Core Imaging Facility
} } American Museum of Natural History
} } Central Park West at 79th Street
} } New York, NY 10024 USA
} }
} } Email: avklaus-at-amnh.org
} } Tel: 212-769-5977



From daemon Tue Jan 29 13:33:41 2002



From: jshields-at-cb.uga.edu
Date: Tue, 29 Jan 2002 14:30:33 -0500
Subject: Southeastern Microscopy Society meeting

Contents Retrieved from Microscopy Listserver Archives
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The Southeastern Microscopy Society will be having its annual
meeting in Athens, Georgia, May 15-17, 2002 at the University of
Georgia campus.
More information can be found at:
http://www.biotech.ufl.edu/sems/

John Shields
EM lab
UGA


From daemon Tue Jan 29 16:27:36 2002



From: John Basgen :      basgen-at-maroon.tc.umn.edu
Date: Tue, 29 Jan 2002 16:20:16 -0600
Subject: Adobe Photoshop Question

Contents Retrieved from Microscopy Listserver Archives
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Listers:

I am using the measure tool of Adobe Photoshop 6.0 to measure distances on my EM
images. I am making multiple measurements on each image. I cannot figure out
how to save these measurements without writing them on a piece of paper. Does
Photoshop save these measurements? If not, is there a plug-in that saves the
measure tool measurements to a spreadsheet?

Go Rams,

John

John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu



From daemon Tue Jan 29 17:15:16 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 29 Jan 2002 18:09:43 -0500
Subject: Adobe Photoshop Question

Contents Retrieved from Microscopy Listserver Archives
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Consider getting John Russ' Image Processing Toolkit for Photoshop.

You can do what you want to do by adding a layer and drawing lines on top of the features that you want to measure. After the micrograph magnification has been calibrated in IPTK, you run the measure features plug-in in IPTK and you will get a text file. You can open the text file in Excel and get a list of all the features of all of the lines that you drew. You are only interested in the length of the lines. It works very well when you want to make a bunch of measurements say of a thickness of film in cross section and average them and find a standard deviation. There are other IPTK tools that actually can give you these values, without going into Excel, but I like to see the actual measurements.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: John Basgen [mailto:basgen-at-maroon.tc.umn.edu]
Sent: Tuesday, January 29, 2002 5:20 PM
To: Microscopy-at-sparc5.microscopy.com


Listers:

I am using the measure tool of Adobe Photoshop 6.0 to measure distances on my EM
images. I am making multiple measurements on each image. I cannot figure out
how to save these measurements without writing them on a piece of paper. Does
Photoshop save these measurements? If not, is there a plug-in that saves the
measure tool measurements to a spreadsheet?

Go Rams,

John

John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu



From daemon Tue Jan 29 17:28:06 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 29 Jan 2002 15:24:21 -0800
Subject: Re: SEM sample prep of Semiconductor Xsect

Contents Retrieved from Microscopy Listserver Archives
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Have you been thrown into the kitchen without any utensils?
Sounds like it. Not a good situation.

At less than 0.25u feature size, the die will most certainly
have barrier metal and probably TiW or TiN plugs. These
die may also use Cu interconnects rather than Al. The ability
to obtain good sections such that vias, runners, poly and
passivation are all resolvable is a big challenge. The most
direct and most accurate method is IMO a focused ion beam.
Unfortunately, these are quite expensive (~$2M new, $600K used).

Larger feature size die can be sectioned with mechanical
tools like those made by Buehler. These tools are not costly.
They are typically set up as a sequential set of lapping
stations (3-4 total) where each one uses progressively smaller
size grinding powder and finally, a polishing powder.

In either case, the final step is "decoration." This is where
the planar edge is selectively etched to recess oxide or metal.
This can be done chemically, but plasma is best all around.
The challenge here is to develop a recipe for recessing
what you want to recess. This can take a bit of doing. An
Oxford ICP-80 is a good tool for this. A key thing to watch
out for is buying a plasma etch tool that cannot and will not
handle corrosive gasses. And don't forget that your gasses
will need to be in a gas cabinet and you may require a
scrubber for exhaust gas.

Typical gasses are CF3, CF4, O2, Ar, N, BCL4, to name a few.
A good oxide etch is CF4+O2. The O2 will kill a non-corrosive
turbo and standard mechanical pump. Handling corrosive
gasses drives the cost up.

Depending on your particular situation, why not outsource
this work? There are many companies that will do this
sectioning for under $200 a die. To get set up yourself
is perhaps going to cost at least $100K + time. If there is
a proprietary IP issue, how about mechanical reduction in-house
with final finishing out-house?

gary g.



At 01:26 PM 1/28/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jan 29 18:20:37 2002



From: DrJohnRuss-at-aol.com
Date: Tue, 29 Jan 2002 19:13:49 EST
Subject: Re: Adobe Photoshop Question

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 1/29/02 5:33:32 PM, basgen-at-maroon.tc.umn.edu writes:

} I am using the measure tool of Adobe Photoshop 6.0 to measure distances
} on my EM images. I am making multiple measurements on each image. I cannot
figure
} out how to save these measurements without writing them on a piece of paper.
} Does Photoshop save these measurements? If not, is there a plug-in that
saves
} the measure tool measurements to a spreadsheet?

The measurement tool in Photoshop 6 does not produce values that are
accessible to a plugin, and I do not know of a way to save them to a file. In
the Image Processing Tool Kit we instead used the approach of allowing the
user to draw lines on the image in any selected color (i.e., something not
present naturally, such as black in a color image or red on a grey scale
picture), and then measure the length of all the lines at once and write them
to a spreadsheet file. The drawback of that method is that the order of the
output values is not the way the user created them, but on the other hand
they can be labelled with numbers and appear on the image so it is easy to
print out a record of what you measured.

John Russ


From daemon Wed Jan 30 01:48:24 2002



From: Mike Mizell :      mizell-at-emispec.com (by way of Nestor J. Zaluzec)
Date: Wed, 30 Jan 2002 01:39:52 -0600
Subject: Course Announcement

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers;
Emispec Systems, Inc. would like to remind you we are hosting a
course on ES Vision, April 15-18, 2002 at our facility in sunny/warm
Tempe, AZ. This course introduces attendees to fully integrated
electron microscopy and microanalysis through Emispec's ES Vision
software. Through advances in software, it is now possible to control
and acquire data from all of the detectors attached to an electron
microscope (SEM/TEM/STEM) through one computer interface. This
complete integration makes for more efficient experimentation, and
opens the door for simultaneous acquisition from multiple detectors.
Emispec's applications laboratory includes an FEI, LEO and JEOL
TEM/STEMs that will be used for hands-on learning by attendees.
Various detectors attached to these microscopes include: BF/DF STEM
detectors, CCD cameras, TV cameras, EDX detectors and EELS
spectrometers. These detectors will be used in combination to
illustrate the fundamentals behind integrated microscopy.
If you are interested in signing up for the course please logon to
Emispecs website www.emispec.com navigate to the news page and select
short course.
If you have any additional questions you can contact us directly.

**********************************************************************
Michael K. Mizell Phone: 480-894-6443 ext 28
Manager of Sales and Marketing Fax: 480-894-6458
Emispec Systems, Inc. Cell: 602-743-2169
2050 Cottonwood Dr. Email: mizell-at-emispec.com
Tempe, AZ 85282
**********************************************************************
Please visit Emispec's website www.emispec.com





From daemon Wed Jan 30 01:48:24 2002



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Date: Tue, 29 Jan 2002 05:22:19 -0700
Subject: WHOLESALE CELLULAR ACCESSORIES FACE PLATES AS LOW AS 2.98 1608

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From daemon Wed Jan 30 04:39:17 2002



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 30 Jan 2002 10:38:07 +0000
Subject: Re: Cacodylate vs. phosphate buffers

Contents Retrieved from Microscopy Listserver Archives
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Angela

I apologise if this has already been mentioned but phosphates also have
a habit of precipitating calcium and magnesium ions which may be added
at various stages during fixation to stabilise lipid based structures in
the specimen (e.g. preservation of spindle fibres, myelin and
membranes). Cacodylate has often been chosen simply because it has
similar fixative vehicle properties to phosphate buffer but without the
precipitation. It is also assumed that it will have better keeping
properties because of its toxicity. I know that it was particularly
popular for a lot of animal tissue fixation and I'm sure that I read
somewhere once that it may cause more extraction giving a clearer cell
matrix and so is better for nice contrasty pictures.

I do still have a bottle of cacodylate locked away but I prefer to use
PIPES (or perhaps HEPES) because of the toxicity of cacodylate. PIPES
seems to be a good general replacement for cacodylate and has none of
the precipitation problems of phosphate. The only problem is that PIPES
is more expensive, but is that really an issue when considering the
safety and disposal of cacodylate?

Malcolm

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (44) (0)191 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk

"akc-at-umich.edu"-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Another minor reason involves convenience. In my experience, the
} concentrated phosphate buffer stock that was stored in the refrigerator
} gradually developed crystals that had to be dissolved before the stock
} could be used. This didn't happen with the concentrated cacodylate buffer
} stock.
}
} Kent
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} A. Kent Christensen, Professor Emeritus
} Department of Cell and Developmental Biology, Medical Science II Building
} University of Michigan Medical School, Ann Arbor, MI 48109-0616
} Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
} akc-at-umich.edu http://www.umich.edu/~akc/
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
} --On Tuesday, January 29, 2002 8:52 AM -0600 "by way of Nestor J. Zaluzec"
} {rcmoretz-at-att.net} wrote:
}
} }
} } Angela:
} } The main reason for using cacodylate instead of
} } phosphate is that uranyl salts precipitate in the
} } presence of phosphate. If one uses phosphate in the
} } original fixative, then several washes into another
} } buffer (e.g. through Tris into cacodylate) is required
} } before finishing. Since I use en bloc staining with
} } uranyl acetate, I prefer to use cacodylate throughout.
} } I also think (based only on my own biased sampling) that
} } I have less uranyl precipitation when staining on grids
} } with cacodylate vs phosphate buffered tissues.
} } Roger
} }
} } --
} } Where the world is only slightly
} } less weird than it actually is.
}
} } } Hi all...
} } }
} } } Sorry for this really basic question:
} } }
} } } Does anyone have any direct experience with using cacodylate vs.
} } } phosphate buffers with glutaraldehyde for fixing spermatozoa? I have
} } } a colleague having some sperm shipped from Africa, and she would like
} } } to use phosphate rather than cacodylate buffer. Also, I'm just
} } } curious as to why one might be superior to the other, barring toxicity
} } } issues. I've always used cacodylate for sperm.
} } }
} } } Best,
} } }
} } } Angela
} } }
} } } Angela V. Klaus, Ph.D.
} } }
} } } Director, Core Imaging Facility
} } } American Museum of Natural History
} } } Central Park West at 79th Street
} } } New York, NY 10024 USA
} } }
} } } Email: avklaus-at-amnh.org
} } } Tel: 212-769-5977


From daemon Wed Jan 30 07:51:45 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 30 Jan 2002 08:37:22 -0500
Subject: Re: FoolProof Alternative to LN2 Filter Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thought I'd see this one by now, but then....

Last time I contaminated an detector Dewar it was because I hadn't cleaned
the transfer Dewar and poured the 'last' little bit into the detector
vessel. So, my solution was to place signs on the detector Dewar and the
transfer Dewar. They read:

Transfer: "Danger, I have hard water in my bottom.

Please clean my bottom regularly!!!"

Detector: "Don't you dare put water from your bottom in
me"!!!

I observed the signs and didn't have any further problem.

Hope this helps.


Frederick C. Monson, PhD
The best research
Center for Advanced Scientific Imaging
occurs before work
West Chester University
at the bench.
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


From daemon Wed Jan 30 07:57:39 2002



From: Roberto Garcia :      rgarcia-at-unity.ncsu.edu
Date: Wed, 30 Jan 2002 08:51:38 -0500
Subject: Re: Adobe Photoshop Question

Contents Retrieved from Microscopy Listserver Archives
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John,

If you want a quick and inexpensive way of getting that information into a
spreadsheet format try UTHSCSA image tool. It is great for making multiple
measurements and the information is stored in a format that excel can read.
And best of all it is free. It was written by the Univ of Texas. You can
find a link to the download page by going to
http://www.ddsdx.uthscsa.edu/dig/itdesc.html . Give it a try and I am sure
you will find that for taking measurements and even for simple image
analysis of objects it is very simple and user friendly.

______________________________
Roberto Garcia
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm




From daemon Wed Jan 30 10:00:50 2002



From: Krishnakumar R :      rkrishnakumar-at-vsnl.net
Date: Wed, 30 Jan 2002 21:24:39 +0530
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Wed Jan 30 10:12:23 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 30 Jan 2002 15:59:55 +0000 (GMT Standard Time)
Subject: Re: Cacodylate vs. phosphate buffers

Contents Retrieved from Microscopy Listserver Archives
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I have been thinking of switching from cacodylate to HEPES
or PIPES (which is better?). Would you mind posting your
HEPES/PIPES protocol?

Dave


On Wed, 30 Jan 2002 10:38:07 +0000 Malcolm Haswell
{malcolm.haswell-at-sunderland.ac.uk} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Angela
}
} I apologise if this has already been mentioned but phosphates also have
} a habit of precipitating calcium and magnesium ions which may be added
} at various stages during fixation to stabilise lipid based structures in
} the specimen (e.g. preservation of spindle fibres, myelin and
} membranes). Cacodylate has often been chosen simply because it has
} similar fixative vehicle properties to phosphate buffer but without the
} precipitation. It is also assumed that it will have better keeping
} properties because of its toxicity. I know that it was particularly
} popular for a lot of animal tissue fixation and I'm sure that I read
} somewhere once that it may cause more extraction giving a clearer cell
} matrix and so is better for nice contrasty pictures.
}
} I do still have a bottle of cacodylate locked away but I prefer to use
} PIPES (or perhaps HEPES) because of the toxicity of cacodylate. PIPES
} seems to be a good general replacement for cacodylate and has none of
} the precipitation problems of phosphate. The only problem is that PIPES
} is more expensive, but is that really an issue when considering the
} safety and disposal of cacodylate?
}
} Malcolm
}
} Malcolm Haswell
} Electron Microscopy
} School of Sciences
} Fleming Building
} University of Sunderland
} SUNDERLAND SR1 3SD
} Tyne and Wear
} UK
}
} Tel (44) (0)191 515 2872
} e-mail: malcolm.haswell-at-sunderland.ac.uk
}
} "akc-at-umich.edu"-at-sparc5.microscopy.com wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Another minor reason involves convenience. In my experience, the
} } concentrated phosphate buffer stock that was stored in the refrigerator
} } gradually developed crystals that had to be dissolved before the stock
} } could be used. This didn't happen with the concentrated cacodylate buffer
} } stock.
} }
} } Kent
} }
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } A. Kent Christensen, Professor Emeritus
} } Department of Cell and Developmental Biology, Medical Science II Building
} } University of Michigan Medical School, Ann Arbor, MI 48109-0616
} } Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
} } akc-at-umich.edu http://www.umich.edu/~akc/
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} }
} } --On Tuesday, January 29, 2002 8:52 AM -0600 "by way of Nestor J. Zaluzec"
} } {rcmoretz-at-att.net} wrote:
} }
} } }
} } } Angela:
} } } The main reason for using cacodylate instead of
} } } phosphate is that uranyl salts precipitate in the
} } } presence of phosphate. If one uses phosphate in the
} } } original fixative, then several washes into another
} } } buffer (e.g. through Tris into cacodylate) is required
} } } before finishing. Since I use en bloc staining with
} } } uranyl acetate, I prefer to use cacodylate throughout.
} } } I also think (based only on my own biased sampling) that
} } } I have less uranyl precipitation when staining on grids
} } } with cacodylate vs phosphate buffered tissues.
} } } Roger
} } }
} } } --
} } } Where the world is only slightly
} } } less weird than it actually is.
} }
} } } } Hi all...
} } } }
} } } } Sorry for this really basic question:
} } } }
} } } } Does anyone have any direct experience with using cacodylate vs.
} } } } phosphate buffers with glutaraldehyde for fixing spermatozoa? I have
} } } } a colleague having some sperm shipped from Africa, and she would like
} } } } to use phosphate rather than cacodylate buffer. Also, I'm just
} } } } curious as to why one might be superior to the other, barring toxicity
} } } } issues. I've always used cacodylate for sperm.
} } } }
} } } } Best,
} } } }
} } } } Angela
} } } }
} } } } Angela V. Klaus, Ph.D.
} } } }
} } } } Director, Core Imaging Facility
} } } } American Museum of Natural History
} } } } Central Park West at 79th Street
} } } } New York, NY 10024 USA
} } } }
} } } } Email: avklaus-at-amnh.org
} } } } Tel: 212-769-5977
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Jan 30 11:34:32 2002



From: Don Gantz :      Gantz-at-med-biophd.bu.edu
Date: Wed, 30 Jan 2002 12:27:49 -0500
Subject: Adobe Photoshop Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John Basgen:
Another software program that you may want to consider for measuring is
ScionImage (freeware available at www.scioncorp.com). Using the "line" tool
you can quickly measure distances which are automatically recorded in a
results window and subsequently exported to Excel.

Go Pats

Donald Gantz
Dept. Physiology & Biophysics
Boston University School of Medicine
Boston, Massachusetts 02118
Email: Gantz-at-Biophysics.bumc.bu.edu
Phone: 617-638-4017




From daemon Wed Jan 30 12:01:56 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Wed, 30 Jan 2002 12:55:26 -0500
Subject: IR microscopy

Contents Retrieved from Microscopy Listserver Archives
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Hi,
One of my customers has a request about IR microscopy which is a little out
of my expertise. I would appreciate hearing from anyone who may have some
information to share on instrumentation which may fulfill this customer's
requirements which to me sound quite impossible. I would also appreciate
hearing from anyone who may provide this expertise as a service.
The requirements read as follows:

"We need an IR microscope that is extremely sensitive to the wavelength of
1.5 um. Ideally it should also cover the visible light so the system can
be used for multiple purposes. We are detecting low intensity light with a
power less than 1 mW. The focal length should be 3 cm or higher and the
field view should be 1 mm or less. It should resolve features less than a
few micros. The system will be used to detect the light distributions among
optical fibers, waveguide and the interconnect area. We have some
components such as monitor, frame et al. in house that can be part of the
system."

Thanks for any information, You can reply to me directly -at-
RGillmeister-at-crt.xerox.com


Russ Gillmeister
Microscopy
Bldg. 114-42D
Xerox Corp.
800 Phillips Rd.
Webster, NY 14580
(585) 422-5317



From daemon Wed Jan 30 12:07:44 2002



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Wed, 30 Jan 2002 12:01:44 -0600
Subject: EM position

Contents Retrieved from Microscopy Listserver Archives
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Opening for an electron microscopist to head a state of the art EM
facility at the Howard Hughes Medical Institute at Rockefeller
University. We seek someone who combines extensive and varied experience
in EM with an intellectual and scientific interest in cell biology. We
study the dynamics of epidermal cell-cell adhesion and the cytoskeleton
in normal and disease states. Position is at level of Senior Res.
Specialist, Res Assoc. or Res. Assoc. Assistant Professor, depending
upon qualifications. Minimum 5 yr. experience in EM, including immunoEM.
Please send CV, reprints and 3 references to: Dr. Elaine Fuchs, Howard
Hughes Medical Institute, 5841 S. Maryland Ave. MC1028, Chicago, IL
60637


From daemon Wed Jan 30 12:30:45 2002



From: Harry Walsh :      h_walsh-at-acs.org
Date: Wed, 30 Jan 2002 13:17:48 -0500
Subject: ACS Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is to ask that you post the following announcement. Thank you for your
help.

----------------------------------------------------------------------------
---------------------------------------------------------
The American Chemical Society will be offering its popular hands-on short
course, Applied Optical Microscopy, on March 15-17, 2002, immediately
preceding PITTCON 2002 in New Orleans, LA. The course is designed for
researchers, technicians, and quality assurance and failure analysis
scientists who need to develop a strong foundation in optical microscopy or
who need to extend their capability in the field. A full course description
can be found in the online catalog at www.chemistry.org/shortcourses.
Scroll down to "What's New" to find the "ACS Short Courses at PITTCON 2002"
catalog which is downloadable as a pdf file. Or, contact the ACS at
shortcourses-at-acs.org or 800-227-5558, ext. 4508. The course registration
fee is $1,095 for ACS members and $1,195 for nonmembers. The course is
strictly limited to 20 participants to ensure time for individual
consultations with the instructors.

----------------------------------------------------------------------------
------------------------------------------------

********************************************************************
Harold G. Walsh
Department of Continuing Education
American Chemical Society
1155 Sixteenth Street, N.W.
Washington, DC 20036

Phone: 800-227-5558, extension 4507, or 202-872-4507
Fax: 202-872-6336
Email: h_walsh-at-acs.org

Visit our web site at www.chemistry.org/shortcourses
********************************************************************



From daemon Wed Jan 30 12:37:52 2002



From: rose evelyn :      roseevelyn-at-thetwinstar.com
Date: Wed, 30 Jan 2002 18:30:23 -0000
Subject: EM safety and family plan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

One of my EM technicians came up with a question---She intends to have a

family, will her everyday duty involving EM influence her pregnancy? I
just
haven't got a clue. Her duty is looking after the users on SEMs and TEMs

facilities, including sample preparations. We are dealing with mostly
inorganic materials (90%) and biological tissues(10%). As we also have
regular admission of female research students (1-3yrs) in the EM center,
it
was time to find out the safety and health for such an issue.

Cheers,
Evelyn



From daemon Wed Jan 30 12:59:16 2002



From: Holly Aaron :      hollya-at-socrates.berkeley.edu
Date: Wed, 30 Jan 2002 10:53:29 -0800
Subject: RE: Adobe Photoshop Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John -

Just my (not so) humble opinion: scrap Photoshop and use NIH-Image or
ImageJ - available FOR FREE at http://rsb.info.nih.gov/nih-image/.

Photoshop, while a lovely program and very useful for color management and
picture touch-ups and more, was never intended to be used for scientific
measurements. Not that it can't be, but as you have found out, it is not
set-up for that purpose. I am less familiar with the new brother ImageJ,
but in NIH-Image all your measurements are written to a text file
automatically. And you can choose *which* measurements are recorded. Then
all you have to do is save the file. From there, you can open it in any
program - Excel, Word, Igor, IDL, LabView, MatLab, etc. With a little
programming you can also create your own analysis package - easiest to do by
using some of the example macros included or downloaded from the website.
And did I mention it was FREE?

Anyway, if you want to know more about how to use Image, send me a note.

Best of luck,
Holly


Holly Aaron
Molecular Imaging Center
http://imaging.berkeley.edu


} -----Original Message-----
} From: John Basgen [mailto:basgen-at-maroon.tc.umn.edu]
} Sent: Tuesday, January 29, 2002 2:20 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Adobe Photoshop Question
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers:
}
} I am using the measure tool of Adobe Photoshop 6.0 to measure
} distances on my EM
} images. I am making multiple measurements on each image. I
} cannot figure out
} how to save these measurements without writing them on a piece of
} paper. Does
} Photoshop save these measurements? If not, is there a plug-in
} that saves the
} measure tool measurements to a spreadsheet?
}
} Go Rams,
}
} John
}
} John M. Basgen
} Department of Pediatrics
} University of Minnesota
} Mayo Mail Code 491
} 420 Delaware Street SE
} Minneapolis, MN 55455
} USA
} Phone: 612-625-7979
} FAX: 612-626-2791
} E-mail: basgen-at-umn.edu
}
}
}



From daemon Wed Jan 30 13:22:43 2002



From: Neusa Nogueira :      nogueira-at-cena.usp.br
Date: Wed, 30 Jan 2002 17:16:35 -300
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




unsubscribe



From daemon Wed Jan 30 14:47:45 2002



From: Anita Garg :      Anita.Garg-at-grc.nasa.gov
Date: Wed, 30 Jan 2002 15:37:26 -0500
Subject: Grain boundary etch for Cu

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues
Does anybody know of an etchant to reveal grain boundaries in Cu and its
alloys?
Any help would be appreciated.
TIA
Anita



From daemon Wed Jan 30 16:52:16 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 30 Jan 2002 17:45:10 -0500
Subject: RE: Adobe Photoshop Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have NIH-Image and Photoshop. I've used them both for a number of years. If you do image analysis infrequently, then in my opinion, NIH-Image is harder to use than the Image Processing Toolkit and Fovea Pro. NIH-Image is also not that easy to program to do some of the things that IPTK and FP can do. For a modest fee, you don't have to reinvent the wheel or have to commit a huge chunk of time.

For what it does and the documentation that comes with it, plus its tie to John's Textbook, Image Processing Handbook, I do not think that it is overpriced and it turns Photoshop into something that can be used in a scientific way. In addition, the plug-ins work in other programs including NIH-Image (although I have not tried them in it.)

Another very important point to consider is that Photoshop works very similarly across both the Mac and PC platform. As someone who works for a company that will not allow any more Macs to be purchased and who goes to other laboratories to do work, this is very important to me. I have used the IPTK on both PC and Mac platforms in Photoshop and have been very pleased.

Another point in favor of IPTK is that you get John Russ's expertise. He's one of us! John monitors this listserver and chirps in regularly. He has always been ready to help with questions of both a scientific nature and on IPTK. He has been committed to teaching Quantitative Microscopy for many years.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Holly Aaron [mailto:hollya-at-socrates.berkeley.edu]
Sent: Wednesday, January 30, 2002 1:53 PM
To: John Basgen; Microscopy-at-sparc5.microscopy.com


Dear John -

Just my (not so) humble opinion: scrap Photoshop and use NIH-Image or
ImageJ - available FOR FREE at http://rsb.info.nih.gov/nih-image/.

Photoshop, while a lovely program and very useful for color management and
picture touch-ups and more, was never intended to be used for scientific
measurements. Not that it can't be, but as you have found out, it is not
set-up for that purpose. I am less familiar with the new brother ImageJ,
but in NIH-Image all your measurements are written to a text file
automatically. And you can choose *which* measurements are recorded. Then
all you have to do is save the file. From there, you can open it in any
program - Excel, Word, Igor, IDL, LabView, MatLab, etc. With a little
programming you can also create your own analysis package - easiest to do by
using some of the example macros included or downloaded from the website.
And did I mention it was FREE?

Anyway, if you want to know more about how to use Image, send me a note.

Best of luck,
Holly


Holly Aaron
Molecular Imaging Center
http://imaging.berkeley.edu


} -----Original Message-----
} From: John Basgen [mailto:basgen-at-maroon.tc.umn.edu]
} Sent: Tuesday, January 29, 2002 2:20 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Adobe Photoshop Question
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers:
}
} I am using the measure tool of Adobe Photoshop 6.0 to measure
} distances on my EM
} images. I am making multiple measurements on each image. I
} cannot figure out
} how to save these measurements without writing them on a piece of
} paper. Does
} Photoshop save these measurements? If not, is there a plug-in
} that saves the
} measure tool measurements to a spreadsheet?
}
} Go Rams,
}
} John
}
} John M. Basgen
} Department of Pediatrics
} University of Minnesota
} Mayo Mail Code 491
} 420 Delaware Street SE
} Minneapolis, MN 55455
} USA
} Phone: 612-625-7979
} FAX: 612-626-2791
} E-mail: basgen-at-umn.edu
}
}
}



From daemon Wed Jan 30 17:10:55 2002



From: R. Ann Bliss :      bliss5-at-popcorn.llnl.gov
Date: Wed, 30 Jan 2002 15:05:35 -0800
Subject: Black art of electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:

Are there any electropolishing gurus out there? I have been working
with a single crystal Ta with a -1,0,1 foil normal. The equipment is
a Fischione Twin Jet polisher. My solution is 350 ml methanol, 150 ml
butoxyethanol, 25 ml sulfuric acid and 5 ml hydrofluoric acid. It is
at -25šC. The voltage is at 80v and the amperage is about 75mA. This
is a new analog power unit.

The sample polishes nicely most of the way through. Then a small
portion starts to extrude and the perforation happens at the peak of
the bump. There are many cracks and bends. These are the same
parameters I have used in the past. This problem is new. I have tried
to lower the temperature by 10 š. The same results, but it took longer

Any ideas?
Thanks in advance,
--

+++++++++++++++++++++++++++++

R. Ann Bliss
Technician, Chemistry and Materials Science
Materials Science and Technology Division
Lawrence Livermore National Laboratory

_____________________________


From daemon Wed Jan 30 17:15:27 2002



From: Kai Lorcharoensery :      kai-at-lehigh.edu
Date: Wed, 30 Jan 2002 18:10:00 -0500
Subject: Re: Grain boundary etch for Cu

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I normally use a mixture of

1 part (volume) of water
1 part of ammonium hydroxide
1 part of 30% hydrogen peroxide

A lot of annealing twins will come up too.

Some people use 3% H2O2 instead of 30%. It will take longer time.
You can search for other etchants at
http://www.kaker.com/etch/demo/search.html. Or take a look in the back
of Vander Voort's "Metallography Principles and Practice"

Kai


From daemon Wed Jan 30 18:41:24 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 30 Jan 2002 14:33:32 -1000 (HST)
Subject: Sputter coaters and BSE detectors

Contents Retrieved from Microscopy Listserver Archives
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Hi, All-

I am interested in hearing opinions, positive and negative, from anyone
who has recently researched or bought a sputter coater or backscattered
electron detector, both destined for use with a Hitachi S-800 FESEM.

Thanks in advance for letting me mine the expertise of the group!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Jan 30 18:56:20 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Thu, 31 Jan 2002 11:53:01 +1100
Subject: solubilities of fixatives

Contents Retrieved from Microscopy Listserver Archives
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Dear microscopists,

Does anyone have to hand, or know where I can look up the solubilities of
fixatives such as paraformaldehyde, glutaraldehyde and osmium tetroxide in
aqueous and a variety of organic solvents? I need to know this for
phase-partition fixation. Have tried handbook of chemistry and physics -
various editions, Merck index, and web sources, but nothing quantitative
enough in these. Perhaps there is a specific chemistry handbook that has
this sort of info?

TIA,
rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475 or 61-0402 835 973
fax 61-2-6246 5000
email rosemary.white-at-csiro.au




From daemon Wed Jan 30 19:20:45 2002



From: K.N. Bozhilov :      bozhilov-at-citrus.ucr.edu
Date: Wed, 30 Jan 2002 17:15:19 -0800
Subject: X-ray Microanalysis Reference Standards for TEM

Contents Retrieved from Microscopy Listserver Archives
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Could any suggest good source/vendor of reference X-ray Microanalysis
standards for TEM for most of the rock-forming elements including Si, Al, K,
Ca, Mg, Mn, Fe, Cr, Ti, Ni, V, Co etc. in oxide or silicate form?

I have tried the so called "Standards" sold by Electron Microscopy Sciences
Inc. and I do not recommend them to anyone who wants to do quantitative
measurements. First all grids were contaminated with salt (NaCl). After I
send the original set back to the company they were polite enough to replace
it with a new one which again was a little bit too "salty" to my taste. The
second even more important problem was that the so called "standards" were
not homogeneous both in terms of concentration and elemental composition.

Also I would be very thankful to anyone who could sent or sell to me crystal
or mineral grains (0.1 grams is plenty) of chemically well characterized and
homogeneous materials containing any set of the above mentioned elements.

Thank you,

Krassimir N. Bozhilov, PhD
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel 909 787 2998
Fax 909 787 4324




From daemon Wed Jan 30 20:37:42 2002



From: Matt Olszta :      molsz-at-mse.ufl.edu
Date: Wed, 30 Jan 2002 21:24:53 -0500
Subject: Micromanipulator

Contents Retrieved from Microscopy Listserver Archives
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We have money in our budget to obtain a micromanipulator. Our work involves
ionic salts, and so it wouldn't have to be anything extremely complicated.
I was wondering if anyone has one that they prefer, and if so, the type of
work that they do on it. Any advice would be much appreciated.

Regards,
Matt Olszta
University of Florida
Department of Materials Science



From daemon Wed Jan 30 22:23:38 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 30 Jan 2002 23:25:02 -0500
Subject: Re: Grain boundary etch for Cu

Contents Retrieved from Microscopy Listserver Archives
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Anita,

The ammonia and peroxide etch already cited on the list is a good one.
Experience will dictate how strong the peroxide should be, generally the
lower the copper content in the alloy, the lower the peroxide concentration.

An alternative is ammonium persulfate in water. Try 10% and vary the
concentration with experience. You may experience precipitate formation
with some alloy ingredients. This can be ameliorated by swabbing during
the etch.

John Twilley
Conservation Scientist

Anita Garg wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues
} Does anybody know of an etchant to reveal grain boundaries in Cu and
} its alloys?
} Any help would be appreciated.
} TIA
} Anita
}
}
}
}



From daemon Wed Jan 30 22:39:28 2002



From: Ampai Sahapattana :      Ampai.S-at-student.chula.ac.th
Date: Thu, 31 Jan 2002 11:33:43 +0700 (GMT+0700)
Subject: SEM:Scanning coil detail

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I'm study Master degree in Chulalongkong university and got an assignment
to study scanning coil of sem. I try to look from internet but can't found
matching info. So please suggest websites or give me more details
about the structure,materials,required theory for understanding it.

Ampai Sahapattana
Nuclear Technology Dept.
Engineering Faculty



From daemon Thu Jan 31 04:16:32 2002



From: Richard Beanland :      richard.beanland-at-marconi.com
Date: Thu, 31 Jan 2002 09:51:37 -0000
Subject: IR microscopy

Contents Retrieved from Microscopy Listserver Archives
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Hi Russ,
we do this kind of thing. I have a polyvar infrapol (pulled out of
a skip - yes, literally! a few years ago). It's a lovely microscope with a
variety of filters you can use for incident light (reflection and
transmission) as well as light leaving the sample before it hits the camera.
Cross polarisers too if you need them. There's a variety of objectives on a
turret so getting the right mag is not a problem.
The camera will have to be a vidicon type, since CCD cameras don't go that
far into the IR. I use a Hamamatsu C2400 to do electroluminescence studies
on 1.5um lasers. You can put the output into a standard video framegrabber,
or use the more expensive Hamamatsu framegrabber which allows you to have
more control over the camera.

Good luck,

Richard


-----Original Message-----
} From: Gillmeister, Russ [mailto:RGillmeister-at-crt.xerox.com]
Sent: 30 January 2002 17:55
To: 'MSA'


Hi,
One of my customers has a request about IR microscopy which is a little out
of my expertise. I would appreciate hearing from anyone who may have some
information to share on instrumentation which may fulfill this customer's
requirements which to me sound quite impossible. I would also appreciate
hearing from anyone who may provide this expertise as a service.
The requirements read as follows:

"We need an IR microscope that is extremely sensitive to the wavelength of
1.5 um. Ideally it should also cover the visible light so the system can
be used for multiple purposes. We are detecting low intensity light with a
power less than 1 mW. The focal length should be 3 cm or higher and the
field view should be 1 mm or less. It should resolve features less than a
few micros. The system will be used to detect the light distributions among
optical fibers, waveguide and the interconnect area. We have some
components such as monitor, frame et al. in house that can be part of the
system."

Thanks for any information, You can reply to me directly -at-
RGillmeister-at-crt.xerox.com


Russ Gillmeister
Microscopy
Bldg. 114-42D
Xerox Corp.
800 Phillips Rd.
Webster, NY 14580
(585) 422-5317



From daemon Thu Jan 31 05:25:49 2002



From: Terry Cooper :      terry.cooper-at-btinternet.com
Date: Thu, 31 Jan 2002 11:10:25 -0000
Subject: TAAB Resin and mineralised collagen

Contents Retrieved from Microscopy Listserver Archives
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Dear Matt,

We at TAAB had a request from a major customer a couple of years ago to
overcome just the problem you describe. We have modified our own epoxy
resin (amazingly called TAAB Embedding Resin!) to have an extreme hardness
yet still maintain the ability to trim and cut easily. It was so successful
with our customer that they now use it for all their TEM embedding
requirements. It comes only as a Premix Kit where all the components are
pre-measured and combined just before use.

Please feel free to contact me for further details,

Best regards

Terry Cooper
TAAB Laboratories Equipment Ltd
Tel ++44 (0)118 981 7775 Fax ++44 (0)118 981 7881
e-mail sales-at-taab.co.uk


From daemon Thu Jan 31 07:22:36 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 31 Jan 2002 07:15:00 -0600
Subject: RE: Sputter coaters and BSE detectors

Contents Retrieved from Microscopy Listserver Archives
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Hi Tina,

I have been using a GW Electronics BSE system on an Etec SEM (now a Hitachi
S-3500) for a long time; the latest model system for just a few years. No
problems/break-downs combined with excellent performance translates to one
very satisfied customer.

I have made a few suggestions for improvement, but if, as a vendor, you ever
sold me some equipment, that would not surprise you {g} .

My Polaron coaters work fine, but are a bit too old to relate to your
inquiry.

Regards,
Woody White
McDermott Technology, Inc.
----------------------------------------------------------------------------
-----

} -----Original Message-----
} From: Tina Carvalho [mailto:tina-at-pbrc.hawaii.edu]
} Sent: Wednesday, January 30, 2002 7:34 PM
} To: Microscopy Listserver
} Subject: Sputter coaters and BSE detectors
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi, All-
}
} I am interested in hearing opinions, positive and negative,
} from anyone
} who has recently researched or bought a sputter coater or
} backscattered
} electron detector, both destined for use with a Hitachi S-800 FESEM.
}
} Thanks in advance for letting me mine the expertise of the group!
}
} Aloha,
} Tina
}
} **************************************************************
} **************
} * Tina (Weatherby) Carvalho *
} tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} **************************************************************
} **************
}
}


From daemon Thu Jan 31 07:33:16 2002



From: Per =?iso-8859-1?Q?H=F6rstedt?= :      per.horstedt-at-pathol.umu.se
Date: Thu, 31 Jan 2002 14:24:01 +0100
Subject: EM-high vacuum: ion pumps

Contents Retrieved from Microscopy Listserver Archives
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Need to have two ion getter pumps regenerated.
Does anybody know if there are any companies/labs in Europe, preferably in
scandinavia, that can do this for me?

Yours sincerely

Per Hörstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of Umeå
S-90187 Umeå
Sweden

per.horstedt-at-pathol.umu.se
phone int-46-90-7851541
fax int-46-90-7851215



From daemon Thu Jan 31 08:47:30 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 31 Jan 2002 08:37:14 -0600
Subject: Re: Cacodylate vs. phosphate buffers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Malcom,

It's nice to know there might be a good alternative to cacodylate. We
typically use phosphate buffer, wherever possible, but it is impossible
when working with fine ultrastructure of otoconia in the inner ear. In
our case, the phosphate buffer seems to "reform" the otoconia, which
are made of calcite. Leave the specimen in the phosphate buffer
solution
for a medium length of time and the clacite pebbles start to shrink and
grow, like the calcium is dissolved off one and deposited on another.
Leave it in long enough and they turn into one big rock!

Karen Pawlowski



Malcolm Haswell wrote:
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Angela
}
} I apologise if this has already been mentioned but phosphates also have
} a habit of precipitating calcium and magnesium ions which may be added
} at various stages during fixation to stabilise lipid based structures in
} the specimen (e.g. preservation of spindle fibres, myelin and
} membranes). Cacodylate has often been chosen simply because it has
} similar fixative vehicle properties to phosphate buffer but without the
} precipitation. It is also assumed that it will have better keeping
} properties because of its toxicity. I know that it was particularly
} popular for a lot of animal tissue fixation and I'm sure that I read
} somewhere once that it may cause more extraction giving a clearer cell
} matrix and so is better for nice contrasty pictures.
}
} I do still have a bottle of cacodylate locked away but I prefer to use
} PIPES (or perhaps HEPES) because of the toxicity of cacodylate. PIPES
} seems to be a good general replacement for cacodylate and has none of
} the precipitation problems of phosphate. The only problem is that PIPES
} is more expensive, but is that really an issue when considering the
} safety and disposal of cacodylate?
}
} Malcolm
}
} Malcolm Haswell
} Electron Microscopy
} School of Sciences
} Fleming Building
} University of Sunderland
} SUNDERLAND SR1 3SD
} Tyne and Wear
} UK
}
} Tel (44) (0)191 515 2872
} e-mail: malcolm.haswell-at-sunderland.ac.uk
}
} "akc-at-umich.edu"-at-sparc5.microscopy.com wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Another minor reason involves convenience. In my experience, the
} } concentrated phosphate buffer stock that was stored in the refrigerator
} } gradually developed crystals that had to be dissolved before the stock
} } could be used. This didn't happen with the concentrated cacodylate buffer
} } stock.
} }
} } Kent
} }
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } A. Kent Christensen, Professor Emeritus
} } Department of Cell and Developmental Biology, Medical Science II Building
} } University of Michigan Medical School, Ann Arbor, MI 48109-0616
} } Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
} } akc-at-umich.edu http://www.umich.edu/~akc/
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} }
} } --On Tuesday, January 29, 2002 8:52 AM -0600 "by way of Nestor J. Zaluzec"
} } {rcmoretz-at-att.net} wrote:
} }
} } }
} } } Angela:
} } } The main reason for using cacodylate instead of
} } } phosphate is that uranyl salts precipitate in the
} } } presence of phosphate. If one uses phosphate in the
} } } original fixative, then several washes into another
} } } buffer (e.g. through Tris into cacodylate) is required
} } } before finishing. Since I use en bloc staining with
} } } uranyl acetate, I prefer to use cacodylate throughout.
} } } I also think (based only on my own biased sampling) that
} } } I have less uranyl precipitation when staining on grids
} } } with cacodylate vs phosphate buffered tissues.
} } } Roger
} } }
} } } --
} } } Where the world is only slightly
} } } less weird than it actually is.
} }
} } } } Hi all...
} } } }
} } } } Sorry for this really basic question:
} } } }
} } } } Does anyone have any direct experience with using cacodylate vs.
} } } } phosphate buffers with glutaraldehyde for fixing spermatozoa? I have
} } } } a colleague having some sperm shipped from Africa, and she would like
} } } } to use phosphate rather than cacodylate buffer. Also, I'm just
} } } } curious as to why one might be superior to the other, barring toxicity
} } } } issues. I've always used cacodylate for sperm.
} } } }
} } } } Best,
} } } }
} } } } Angela
} } } }
} } } } Angela V. Klaus, Ph.D.
} } } }
} } } } Director, Core Imaging Facility
} } } } American Museum of Natural History
} } } } Central Park West at 79th Street
} } } } New York, NY 10024 USA
} } } }
} } } } Email: avklaus-at-amnh.org
} } } } Tel: 212-769-5977



From daemon Thu Jan 31 10:09:34 2002



From: Lara.Sciaraffa-at-abbott.com
Date: Thu, 31 Jan 2002 08:36:49 -0600
Subject: Prepared Lead Citrate Stability Data

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,
I am searching for well documented data on the shelf life/stability of
prepared lead citrate (premixed). I am using the Fahmy method for the
preparation. Has anyone had good results using this method? Right now we
are labeling it for daily use, due to vague and incomplete stability data.
*Long shelf life*, is insufficient for GMP labs. Thanks in advance for your
assistance.

Best Wishes,
Lara


Lara A. Sciaraffa, Microscopist
Microscopy & Microanalysis
Dept R45M, Bldg. AP31
200 Abbott Park Road
Abbott Park, IL 60064-6202

Lara.Sciaraffa-at-Abbott.Com



From daemon Thu Jan 31 10:19:19 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 31 Jan 2002 11:12:19 -0500
Subject: Re: EM safety and family plan

Contents Retrieved from Microscopy Listserver Archives
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HI Evelyn,
this question came up a while back,so you may want to scan the
archives, but here are my 2 cents...
I have a healthy, happy 9 year old son. I have been doing TEM & SEM
for 25 years. While I was "family pIanning" and pregnant, I wore
double gloves and worked in the hood when appropriate, washed my
hands frequently, followed OSHA guidelines and used common sense. I
still do (except for the double gloves part). Yes, much of what we
use can be dangerous to ourselves as well as our progeny, but with
appropriate care I don't think one needs to take a leave or stop
doing your job.

JMHO,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Jan 31 11:03:05 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 31 Jan 2002 11:58:04 -0500
Subject: Re: solubilities of fixatives

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Dear Rosemary:

Short answer: I don't know. I think you need to ask a chemist where to
find this information or how to determine this yourself. Hmmm. Sounds like a
good senior project for some chem student?
Long answer: The concentrations of fixatives in aqueous solutions will
equilibrate with an organic phase, until 1. an equilibrium is reached or 2.
the organic phase is saturated. I don't know the time factor involved but an
initial shaking in a separtory funnel followed by a few hours of standing
should be sufficient. Glutaraldehyde can be purchased in aqueous solutions as
concentrated as 70%. You could make your own concentrated paraformaldehyde
solutions, I would think 20% would be more than sufficient. I seem to remember
someone, somewhere put 40% (yes, forty) osmium in carbon tetracholride.
What are you fixing?

Rosemary White wrote:

} Dear microscopists,
}
} Does anyone have to hand, or know where I can look up the solubilities of
} fixatives such as paraformaldehyde, glutaraldehyde and osmium tetroxide in
} aqueous and a variety of organic solvents? I need to know this for
} phase-partition fixation. Have tried handbook of chemistry and physics -
} various editions, Merck index, and web sources, but nothing quantitative
} enough in these. Perhaps there is a specific chemistry handbook that has
} this sort of info?
}
} TIA,
} rosemary
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} phone 61-2-6246 5475 or 61-0402 835 973
} fax 61-2-6246 5000
} email rosemary.white-at-csiro.au

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Jan 31 12:19:34 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Thu, 31 Jan 2002 10:03:12 -0800
Subject: Re: X-ray Microanalysis Reference Standards for TEM

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Dear Krassimir:

You may want to try Geller MicroAnalytical. They have a long list of reference
materials at http://www.gellermicro.com/std-list.pdf.

I hope this helps.

David

"K.N. Bozhilov" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Could any suggest good source/vendor of reference X-ray Microanalysis
} standards for TEM for most of the rock-forming elements including Si, Al, K,
} Ca, Mg, Mn, Fe, Cr, Ti, Ni, V, Co etc. in oxide or silicate form?
}
} I have tried the so called "Standards" sold by Electron Microscopy Sciences
} Inc. and I do not recommend them to anyone who wants to do quantitative
} measurements. First all grids were contaminated with salt (NaCl). After I
} send the original set back to the company they were polite enough to replace
} it with a new one which again was a little bit too "salty" to my taste. The
} second even more important problem was that the so called "standards" were
} not homogeneous both in terms of concentration and elemental composition.
}
} Also I would be very thankful to anyone who could sent or sell to me crystal
} or mineral grains (0.1 grams is plenty) of chemically well characterized and
} homogeneous materials containing any set of the above mentioned elements.
}
} Thank you,
}
} Krassimir N. Bozhilov, PhD
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} Tel 909 787 2998
} Fax 909 787 4324
}

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Thu Jan 31 13:26:34 2002



From: Raghavan Narayanan :      narayanr-at-ecn.purdue.edu
Date: Thu, 31 Jan 2002 14:18:17 -0500
Subject: Re: [Fwd: Fwd: Grain boundary etch for Cu]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Copper etch procedure:

1. For normal etching I use 2 solutions:
i. Mixture of ditilled water or ethanol (100-120 ml),FeCl3(5-10 ml) and
HCl(20-50 ml). Apply this on the polished surface for 20-25 seconds and
wash it thoroughly
ii. Then, use a 50%nitric acid+50%water solution and apply this on the
surface for 10-15 seconds.

2. (OPTIONAL) Before etching, if you want a stress free surface then do
the following. Take a solution of one part acetic acid, 2 parts nitric
acid and 1 part phosphoric acid (H3PO4). Heat the solution to 70 deg C
and immerse the copper specimen for 5-10 seconds. This will remove all
surface stresses and leave a shiny surface.

-Raghavan


From daemon Thu Jan 31 13:38:30 2002



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Date: Tue, 29 Jan 2002 05:22:19 -0700
Subject: WHOLESALE CELLULAR ACCESSORIES FACE PLATES AS LOW AS 2.98 1608

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From daemon Thu Jan 31 13:58:43 2002



From: kellymcg-at-seas.upenn.edu
Date: Thu, 31 Jan 2002 14:53:19 -0500 (EST)
Subject: imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to find a way to have photoshop automatically put scale bars on SEM
images when given certain information (ie magnification and/or image size). IF
anyone knows how to do this or has any suggestions the help would be
appriciated.
thanks
kelly
kellymcg-at-seas.upenn.edu


From daemon Thu Jan 31 14:57:45 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 31 Jan 2002 15:35:00 -0500
Subject: X-ray Microanalysis Reference Standards for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The best analytical standards that I have made for TEM have been by taking powders of known bi-metal oxide, sulphide, or nitride powders and microtoming them. If you are in a hurry, crush them and collect them on a carbon coated grid and only do the data collection in areas that show kinematical contrast if they are crystalline. Unfortunately, they are hard to come by. We have different glass compositions that we have carefully measured their compositions with the microprobe and I have used them as standards.

I have used the EMS standards because they are the only samples available. However, I would not discount them entirely. You do have to look around for both the correct phase and suitable thickness for them to be reproducible.

NIST has glass samples available. Again, I suggest that you use microtoming to prepare them because it is least likely to change the composition compared to ion milling or other methods. They have done a correlation of particle size and microprobe measured microprobe results. Sorry-I can't remember the reference or the NIST numbers for the glasses.

You also have to be very careful with some of the elements in your list in inorganic samples. Several will migrate under the beam and it is aggravated by decreasing the probe size. For example, you will not see Na, Ca, and Mg with a 20-50 Angstrom size probe, but they do show up at about 100 Angstrom. No telling what the concentration is because they are still migrating.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: K.N. Bozhilov [mailto:bozhilov-at-citrus.ucr.edu]
Sent: Wednesday, January 30, 2002 8:15 PM
To: Microscopy-at-sparc5.microscopy.com


Could any suggest good source/vendor of reference X-ray Microanalysis
standards for TEM for most of the rock-forming elements including Si, Al, K,
Ca, Mg, Mn, Fe, Cr, Ti, Ni, V, Co etc. in oxide or silicate form?

I have tried the so called "Standards" sold by Electron Microscopy Sciences
Inc. and I do not recommend them to anyone who wants to do quantitative
measurements. First all grids were contaminated with salt (NaCl). After I
send the original set back to the company they were polite enough to replace
it with a new one which again was a little bit too "salty" to my taste. The
second even more important problem was that the so called "standards" were
not homogeneous both in terms of concentration and elemental composition.

Also I would be very thankful to anyone who could sent or sell to me crystal
or mineral grains (0.1 grams is plenty) of chemically well characterized and
homogeneous materials containing any set of the above mentioned elements.

Thank you,

Krassimir N. Bozhilov, PhD
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel 909 787 2998
Fax 909 787 4324




From daemon Thu Jan 31 15:26:02 2002



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Thu, 31 Jan 2002 13:20:06 -0800
Subject: RE: X-ray Microanalysis Reference Standards for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Krassimir,
Have you considered ordering some of the SRM standards from NIST,
and then preparing your own TEM standards from them? SRM 1412 might have
some of the components you are looking for. Here is a web link for several
oxide SRM produced by NIST:

http://srmcatalog.nist.gov/srmcatalog/tables/112-3.htm


-Brad

----------
From: K.N. Bozhilov
Sent: Wednesday, January 30, 2002 5:15 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: X-ray Microanalysis Reference Standards for TEM


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Could any suggest good source/vendor of reference X-ray
Microanalysis
standards for TEM for most of the rock-forming elements including
Si, Al, K,
Ca, Mg, Mn, Fe, Cr, Ti, Ni, V, Co etc. in oxide or silicate form?

I have tried the so called "Standards" sold by Electron Microscopy
Sciences
Inc. and I do not recommend them to anyone who wants to do
quantitative
measurements. First all grids were contaminated with salt (NaCl).
After I
send the original set back to the company they were polite enough to
replace
it with a new one which again was a little bit too "salty" to my
taste. The
second even more important problem was that the so called
"standards" were
not homogeneous both in terms of concentration and elemental
composition.

Also I would be very thankful to anyone who could sent or sell to me
crystal
or mineral grains (0.1 grams is plenty) of chemically well
characterized and
homogeneous materials containing any set of the above mentioned
elements.

Thank you,

Krassimir N. Bozhilov, PhD
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel 909 787 2998
Fax 909 787 4324






From daemon Thu Jan 31 15:31:31 2002



From: DrJohnRuss-at-aol.com
Date: Thu, 31 Jan 2002 16:25:33 EST
Subject: Re: imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 1/31/02 3:03:25 PM,
kellymcg-at-seas.upenn.edu-at-sparc5.microscopy.com writes:

} I am trying to find a way to have photoshop automatically put scale bars
} on SEM images when given certain information (ie magnification and/or image
size).
} IF anyone knows how to do this or has any suggestions the help would be
} appriciated.

One of the (many) functions of The Image Processing Tool Kit (a set of
photoshop-compatible plug-ins for serious image analysis). See
http://reindeergraphics.com



From daemon Thu Jan 31 16:19:31 2002



From: Eric Steel :      eric.steel-at-nist.gov
Date: Thu, 31 Jan 2002 19:13:20 -0500
Subject: Re: X-ray Microanalysis Reference Standards for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is an older posting of mine that describes a method for putting scale bars on micrographs. For an SEM or a TEM, you could do the same thing at the various mags that you would use routinely. The scale markers that I created look black on white just like the transfer lettering I used. I have put how to do that at the very end of this message because that too was a previous message.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

______________



I am trying to find a way to have photoshop automatically put scale bars on SEM
images when given certain information (ie magnification and/or image size). IF
anyone knows how to do this or has any suggestions the help would be
appriciated.
thanks
kelly
kellymcg-at-seas.upenn.edu


_________________________________



There is a NIST Standard Reference Material (SRM) specifically designed for
calibrating the relative sensitivity factors for X-ray analysis on the
TEM/STEM. I have a bias in my answer in that I helped to prepare the
material as part of my job at NIST so that I had a "monetary interest" in
its production, but get no remuneration for sales.

Here is a brief description of the material, so that you can see if you
might be interested:

http://srmcatalog.nist.gov/srmcatalog/tables/103-3.htm

The standard is called "SRM 2063a Microanalysis Thin Film" and consists of a
thin film of sputtered mineral glass supported by a carbon film and a copper
TEM grid. The composition is certified as listed below and was determined
by several independent techniques after the glass was deposited on the
grids. Thus the certified composition values take into account sample
preparation. The thickness of the glass (76 nm) and density (3.1 gm/cm**3)
are reported (though not certified). No preparation is necessary for use in
the TEM.

Table of Concentration Values for SRM 2063a Mineral Glass
Element Concentration Uncertainty
(% wt.) (% wt.)
O 43.2 1.6
Mg 7.97 0.34
Si 25.34 0.98
Ca 11.82 0.37
Fe 11.06 0.88

The composition allows for a range of relative sensitivity values to be
determined for many commonly analyzed elements and x-ray lines. Thus the
standard can be a primary, traceable way of checking other in-house
standards you may use. The standard is robust under many handling and beam
conditions, though very high beam dose may cause a change in composition
(as noted on the certificate of analysis.) Typically we spread the TEM
beam out over roughly 10 micrometers or use a STEM raster of similar
dimensions during spectral accumulation. We have used one standard grid
for over 10 years in our laboratory to monitor/compare several instruments
and detectors. I have not seen any particular instability or stress
problems in the standard -- even the Ar has remained stable after many years.


For more information about obtaining the SRM 2063a standard you may contact:

Standard Reference Materials Program
National Institute of Standards and Technology
Gaithersburg, MD 20899-0001
USA

Phone: 301-975-6776
FAX: 301-948-3730
e-mail: SRMINFO-at-enh.nist.gov


Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-417-1321
100 Bureau Drive, Stop 8371
Gaithersburg, MD 20899-8371
http://www.nist.gov/micro



From daemon Thu Jan 31 20:21:51 2002



From: Chaoying Ni :      cni-at-udel.edu
Date: Thu, 31 Jan 2002 21:14:45 -0500 (EST)
Subject: TEM lab temperature, humidity, pressure, and field mornitoring

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

I'm running a multi-user EM facility at the college of Engineering,
University of Delaware. We feel our FETEM does not perform fully to the
specs possibly due to some issues with the location and/or construction of
the lab. So, even though I do record temperature and humidity on daily
basis, I have been strongly suggested to set up a monitoring system to
record continuously (preferably multi-points) the lab temperature,
humidity, fluctuation of pressure differential, and electro-magnetic
field. I was wondering if any of you had the experience with the set-up of
such a system, or the similar. Please advise. Thanks very much.

Chaoying Ni
W.M. Keck Electron Microscopy Facility
College of Engineering
University of Delaware
Newark, DE 19716
(302) 831-8354 (Phone)
(302) 831-4545 (Fax)
http:/eml.masc.udel.edu



From daemon Thu Jan 31 21:09:46 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Fri, 1 Feb 2002 14:07:41 +1100
Subject: Re: solubilities of fixatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Geoff,

We've been fixing desiccated plant material, part of a project figuring out
how best to deal with this sort of material. We'd like to know what
concentration of fixative from aqueous solution ends up in the organic
solvent. Hence the question about exact solubilities for the fixatives.
There is some info for OsO4 in "Solubilities of inorganic and organic
compounds", but only for water and CCl4, and general info for formaldehyde,
paraformaldehyde and glutaraldehyde. And from Handbook of Chemistry and
Physics, you get info on relative solubilities in water and a few organic
solvents. But, as you note, to work out the concentration of fix that ends
up in the organic solvent, we need numbers on these solubilities. An
additional complication is that we don't know the exact composition of some
of the perfluorocarbon solvents we've tried.

I suspect we'll either have to be vague about how much fix is in the
solvents, or interest a chemist.....
Rosemary

} Dear Rosemary:
}
} Short answer: I don't know. I think you need to ask a chemist where to
} find this information or how to determine this yourself. Hmmm. Sounds like a
} good senior project for some chem student?
} Long answer: The concentrations of fixatives in aqueous solutions will
} equilibrate with an organic phase, until 1. an equilibrium is reached or 2.
} the organic phase is saturated. I don't know the time factor involved but an
} initial shaking in a separtory funnel followed by a few hours of standing
} should be sufficient. Glutaraldehyde can be purchased in aqueous solutions as
} concentrated as 70%. You could make your own concentrated paraformaldehyde
} solutions, I would think 20% would be more than sufficient. I seem to remember
} someone, somewhere put 40% (yes, forty) osmium in carbon tetracholride.
} What are you fixing?
}
} Rosemary White wrote:
}
} } Dear microscopists,
} }
} } Does anyone have to hand, or know where I can look up the solubilities of
} } fixatives such as paraformaldehyde, glutaraldehyde and osmium tetroxide in
} } aqueous and a variety of organic solvents? I need to know this for
} } phase-partition fixation. Have tried handbook of chemistry and physics -
} } various editions, Merck index, and web sources, but nothing quantitative
} } enough in these. Perhaps there is a specific chemistry handbook that has
} } this sort of info?
} }
} } TIA,
} } rosemary
} }
} } Rosemary White
} } Microscopy Centre
} } CSIRO Plant Industry
} } GPO Box 1600
} } Canberra, ACT 2601
} } Australia
} }
} } phone 61-2-6246 5475 or 61-0402 835 973
} } fax 61-2-6246 5000
} } email rosemary.white-at-csiro.au
}
} Geoff
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************





From daemon Thu Jan 31 22:15:03 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 1 Feb 2002 17:06:41 GMT+1200
Subject: Video Camera on JEOL 840 OM

Contents Retrieved from Microscopy Listserver Archives
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Further to my questions last year, I have now managed to mount
a cheap video camera on to the rather awkwardly-situated optical
microscope of my JEOL 840.

Thanks to those who offered suggestions.

If anyone wants to know how, please contact me off-list.

Has anyone managed to arrange transmitted-light illumination for an
840?

cheers

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Fri Feb 1 02:06:50 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 31 Jan 2002 23:59:24 -0800
Subject: Re: solubilities of fixatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Rosemary

Solubility depends from may parameters, I would suggest 'just to try'. Mix
your fixatives with solvent, see what happening and publish the
results. We will thank you for such job. Sergey

At 07:07 PM 1/31/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Feb 1 04:14:19 2002



From: Torsten.Fregin-at-zoologie.uni-hamburg.de
Date: Fri, 1 Feb 2002 11:06:18 +0100
Subject: Thick sections for confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

I am looking for a method to cut thick sections (50 to 100 µm) from
crustaceans (2-4 cm in length), which can be incubated with
antibodies and viewed on a confocal microscope.

So far I use(d) gelatin-embedded specimens, cut on a kryostat.
The problem is, that when these thick sections get thawed, much
of the animal just "swims" away, because e.g. nerves and vessels
are not attached to something anymore, but float freely in the
water/hemolymph.

To avoid this, I'd like to embedd the animals into something. Here's
a list of thoughts, and reasons why I rejected them:

- gelatin is just not fluid enough to penetrate all hemolymph space;
- celloidin needs a preincubation w/ the antibodies, but this is just
not possible (because the animals are quite large, and the
antibodies e.g. for receptors quite expensive...);
- nanoplast (a water soluble resin) works just like celloidin;
- LR White (thermally cured) seems to be too hard for thick
sections; at least I just cannot get thicker sections then 2 µm with
my microtom and glass knives (and not 10 µm as in the literature).

If someone knows a nice method, I would appreciate help ! ! !

Truly yours,

:-) Torsten

Ph.D. student





Torsten Fregin

Universität Hamburg - Zoologisches Institut
Abt. Neurophysiologie
AG Wiese - Raum 413
Martin-Luther-King-Platz 3
20146 Hamburg, Germany
Telefon *49-(0)40-42838-3931
Fax *49-(0)40-42838-3937
eMail Torsten.Fregin-at-zoologie.uni-hamburg.de
oder TorstenFregin-at-gmx.de




From daemon Fri Feb 1 07:33:31 2002



From: Michiel De Mol :      mdemol-at-uni-hohenheim.de
Date: Fri, 01 Feb 2002 14:20:28 +0100
Subject: Embedding wood with Mistletoe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everybody,

We're trying for months now to embed wood infected with mistletoe for
making sections with a microtome.
We mostly used Technovit, but the plastic infiltrates not deep enough in
the woody tissue, which leeds to holes in the sections.
Sections 5 micron would be fantastic, but sections of 20 micron would do
the job.

Any thoughts, ideas or remarks are welcome!!!

Michiel De Mol
University of Hohenheim
Institute of Botany (210)
Garbenstrasse 30
70599 Stuttgart
Germany




From daemon Fri Feb 1 07:33:31 2002



From: Stanley L. Flegler :      flegler-at-pilot.msu.edu
Date: Fri, 01 Feb 2002 08:25:25 -0500
Subject: Fujifilm Imaging Plate System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of any US distributors for the Fujifilm FDL 5000 Imaging
Plate System? Any leads would be greatly appreciated. Thanks in advance.
Stanley L. Flegler, Director
Center for Advanced Microscopy
Michigan State University


From daemon Fri Feb 1 07:51:10 2002



From: R. Cross :      r.cross-at-ru.ac.za
Date: Fri, 1 Feb 2002 15:45:33 +0200
Subject: ICEM-15 abstract deadline

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


15th International Congress on Electron Microscopy (ICEM-15)

* submission of abstracts - new deadline

Many appeals for concessions are being received from prospective
delegates who, for reasons such as holidays at this time of year
and beginning-of-year commitments, have had difficulty meeting the
1 February deadline for submission of abstracts. Consequently, the
Organizing Committee has agreed to extend the deadline to 25
FEBRUARY.

Please note that this new deadline cannot be extended.


Robin H Cross
Chairman : ICEM-15


From daemon Fri Feb 1 08:01:02 2002



From: joe.p.neilly-at-abbott.com
Date: Fri, 1 Feb 2002 07:54:19 -0600
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I like to use the actions in Photoshop for placing scale bars on light
micrographs, but this only works in version 6.0 or higher. I have several
actions set up to automatically place the bar and corresponding dimension
below it. Each action is designed to place a scale bar on an image at a
specific magnification. Thus, I have many actions for all the magnifications
that I commonly use. Once you set up the action, you can run them with one
click. If you have many images taken at the same magnification, you can run
the action on all of them at once in the batch mode. It is important to run
the scale bar action before you do any resizing of the images. As a double
check I often run the actions on an image of a magnification standard to
confirm that the scale bars are correct.

Joe Neilly, Senior Microscopist
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com



"kellymcg-at-seas.
upenn.edu" To:
Microscopy-at-sparc5.microscopy.com
cc:
01/31/02 01:53 Subject: imaging
PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am trying to find a way to have photoshop automatically put scale bars on
SEM
images when given certain information (ie magnification and/or image size).
IF
anyone knows how to do this or has any suggestions the help would be
appriciated.
thanks
kelly
kellymcg-at-seas.upenn.edu






From daemon Fri Feb 1 08:50:12 2002



From: Larry Allard :      L2A-at-ornl.gov
Date: Fri, 01 Feb 2002 09:40:08 -0500
Subject: Re: Fujifilm Imaging Plate System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Stan:

John Wheatley recently provided me with the following contact:

Fuji Medical Systems U.S.A., Inc.
333Ludlow Street, P. O. Box 120035
Stamford, CT 06912-0035
1-800-446-5450 Ext. 6112
FAX (203) 327-6485


hope this helps.

Larry




At 8:25 AM -0500 2/1/02, Stanley L. Flegler wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Fri Feb 1 12:26:23 2002



From: Michiel De Mol :      mdemol-at-uni-hohenheim.de
Date: Fri, 01 Feb 2002 19:12:57 +0100
Subject: Re: Embedding wood with Mistletoe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tamara,

That is certainly not a dumb question, but it doesn't work. The problem is
that the mistletoe tissue is to soft, relative to te wood, to make fresh
sections with a sliding microtome. The mistletoe tissue would be torn out. And
if that is not the case, the bark or the cambium of the tree would be damaged.

As for the hand sections: we want to make a 3D reconstructon of the endophyte
of the Mistletoe, so we need rather thin serial sections.

We have tried infiltrating at room temperature, but also at 4°C. The
infiltration steps we used, coming from Ethanol 100%, are: Ethanol/Technovit
2/1, 1/1, 1/2, Technovit 100%. The longest infiltration times we tried are 48h
and the last step for 4 days.
After each transfer, we infiltrated under vacuum for 15 min, any longer would,
we think, damage the soft tissues.

Anyway thank you for thinking with me

Michiel De Mol
University of Hohenheim
Institute of Botany (210)
Garbenstrasse 30
70599 Stuttgart
Germany




From daemon Fri Feb 1 14:11:07 2002



From: jesus.echeverria-at-unavarra.es (by way of Nestor J. Zaluzec)
Date: Fri, 1 Feb 2002 14:03:47 -0600
Subject: Ask-A-Microscopist: heavy metals on clay minerals

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jesus.echeverria-at-unavarra.es) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
February 1, 2002 at 02:39:26
---------------------------------------------------------------------------

Email: jesus.echeverria-at-unavarra.es
Name: Dr. J. EcheverrÌa

Organization: Universidad P™blica de Navarra (Spain)

Education: Graduate College

Location: Pamplona, Navarra, Spain

Question: We are studying the retention on heavy metals on clay
minerals (illite). We know that at a given pH, metals precipitate.
So, we would like to visualize the formation of new phases. Our
question is, what is the best way to prepare the sample for scanning
electron microscopy?

---------------------------------------------------------------------------


From daemon Fri Feb 1 14:11:09 2002



From: pollyr-at-cheque.uq.edu.au (by way of Nestor J. Zaluzec)
Date: Fri, 1 Feb 2002 14:02:38 -0600
Subject: Ask-A-Microscopist: observe gel structures in a confectionery

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (pollyr-at-cheque.uq.edu.au) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
January 31, 2002 at 20:29:14
---------------------------------------------------------------------------

Email: pollyr-at-cheque.uq.edu.au
Name: Polly Ramesh Sukha

Organization: University of Queensland

Education: Graduate College

Location: Brisbane, Queensland, Australia

Question: I would like to observe gel structures in a confectionery
jelly that contains both starch gel structures and gelatine
structures. What would be the appropriate method to use to observe
these structures, with regards to staining and microscopy method?

---------------------------------------------------------------------------


From daemon Fri Feb 1 14:15:47 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 1 Feb 2002 15:08:15 -0500
Subject: RE: Thick sections for confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
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Hi Torsten,
I notice that you have not mentioned PEG (polyethylene glycol) or
diethylene glycol distearate (DGD). You might want to look in "PEG as an
embedment.....", Gao, K.(ed), 1993, CRC Press, ISBN: 0-8493-4323-2. Also,
if you have tried gelatin, how about polyacrylamide. These are used for
cryotomy.
Neither have you mentioned glycol methacrylate which is often used
as the dehydrator and infiltrator, provides celloidin-like support without
the miscibility problems and can be thick sectioned. It should hold
structures in place and should permit economy of antibody as it is
hydrophilic.
Cutting thick sections from non-brittle embedment is best done with
a sledge microtome which mounts the knife at an angle to its movement. This
permits a 2-axis sectioning motion that has worked well for taking sections
of small mammals that are partly ossified.
How these may affect confocal imaging I can't predict, but....
Just some more ideas to exclude, but in sum probably worth at least
an EUD.

Regards,

Fred Monson

Frederick C. Monson, PhD
The best research
Center for Advanced Scientific Imaging
occurs before work
West Chester University
at the bench.
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu



} ----------
} From:
} "Torsten.Fregin-at-zoologie.uni-hamburg.de"-at-sparc5.microscopy.com
} Sent: Friday, February 1, 2002 5:06 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Thick sections for confocal microscopy
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi everyone,
}
} I am looking for a method to cut thick sections (50 to 100 µm) from
} crustaceans (2-4 cm in length), which can be incubated with
} antibodies and viewed on a confocal microscope.
}
} So far I use(d) gelatin-embedded specimens, cut on a kryostat.
} The problem is, that when these thick sections get thawed, much
} of the animal just "swims" away, because e.g. nerves and vessels
} are not attached to something anymore, but float freely in the
} water/hemolymph.
}
} To avoid this, I'd like to embedd the animals into something. Here's
} a list of thoughts, and reasons why I rejected them:
}
} - gelatin is just not fluid enough to penetrate all hemolymph space;
} - celloidin needs a preincubation w/ the antibodies, but this is just
} not possible (because the animals are quite large, and the
} antibodies e.g. for receptors quite expensive...);
} - nanoplast (a water soluble resin) works just like celloidin;
} - LR White (thermally cured) seems to be too hard for thick
} sections; at least I just cannot get thicker sections then 2 µm with
} my microtom and glass knives (and not 10 µm as in the literature).
}
} If someone knows a nice method, I would appreciate help ! ! !
}
} Truly yours,
}
} :-) Torsten
}
} Ph.D. student
}
}
}
}
}
} Torsten Fregin
}
} Universität Hamburg - Zoologisches Institut
} Abt. Neurophysiologie
} AG Wiese - Raum 413
} Martin-Luther-King-Platz 3
} 20146 Hamburg, Germany
} Telefon *49-(0)40-42838-3931
} Fax *49-(0)40-42838-3937
} eMail Torsten.Fregin-at-zoologie.uni-hamburg.de
} oder TorstenFregin-at-gmx.de
}
}
}
}


From daemon Fri Feb 1 14:25:37 2002



From: dharvey-at-inplane.com ()
Date: Fri, 1 Feb 2002 14:21:59 -0600
Subject: Ask-A-Microscopist: nemarsky microscopic techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dharvey-at-inplane.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
February 1, 2002 at 12:55:23
---------------------------------------------------------------------------

Email: dharvey-at-inplane.com
Name: Doug Harvey

Organization: Inplane Photonics Inc.

Education: Graduate College

Location: South Plainfield, NJ 07080

Question: Hello, my background is not in optics. I would like to get
more information on using nemarsky microscopic techniques for
measuring the depth of features, e.g. the rounding of circuit traces.
Would you have any information or could point me in the right
direction?

Regards,
Doug

---------------------------------------------------------------------------


From daemon Fri Feb 1 15:06:18 2002



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Fri, 01 Feb 2002 12:59:37 -0800
Subject: Re: Thick sections for confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Celloidin will only need pre-incubation for epitopes denatured by ethanol
and ether. I suspect that if your epitopes can withstand paraffin
embedding, they should tolerate celloidin. The downside will be shrinkage,
especially with soft tissues within rigid structures. Section celloidin
with a slege microtome. sections can be cut, set onto disks of filter
paper then stacked in a jar full of 70% isopropanol or terpineol for later
use.

I've used the rubber cement-paraffin mixture. Its like embedding in Noxema
cream, weird texture but very easy to cut on a slege and dissolves out
with xylene.

} - celloidin needs a preincubation w/ the antibodies, but this is just
} not possible (because the animals are quite large, and the
} antibodies e.g. for receptors quite expensive...);

--
---
Glen MacDonald
Center for Communication Research
Box 357923
University of Washington
Seattle, WA 98195-7923
USA
(206) 616-4156
glenmac-at-u.washington.edu




From daemon Fri Feb 1 15:08:27 2002



From: Doug Cromey :      Cromey-at-Arizona.edu
Date: Fri, 01 Feb 2002 14:03:49 -0700
Subject: LM: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

We have an Olympus IMT-2 set up for time lapse imaging using DIC. During
our over-night exposures we find that the lamp does not provide consistent
illumination, dimming occasionally. This makes for somewhat less than
satisfactory movies. Is there a way we could make the lamp more stable?

Details: 50W tungsten bulb, electrical power goes through a UPS before it
enters the microscope, the lamp is typically at a setting of about 10/12 on
the LCD, I will concede that the UPS is old & I'm unsure of the state of
the batteries, although I'm inclined more to believe its the lamp or lamp
housing.

Thanks for your comments.

Doug

....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: Cromey-at-Arizona.edu) :
:...................................................................:
http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Fri Feb 1 17:39:40 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 01 Feb 2002 15:05:22 -0800
Subject: Re: Embedding wood with Mistletoe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Michiel,
I would suggest you use a low viscosity resin such as Spurr's and apply
vacuum three times to get the thin resin into the pores of your wood.
At 02:20 PM 2/1/02 +0100, you wrote:
}
} Hello everybody,
}
} We're trying for months now to embed wood infected with mistletoe for
} making sections with a microtome.
} We mostly used Technovit, but the plastic infiltrates not deep enough in
} the woody tissue, which leeds to holes in the sections.
} Sections 5 micron would be fantastic, but sections of 20 micron would do
} the job.
}
} Any thoughts, ideas or remarks are welcome!!!
}
} Michiel De Mol

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Fri Feb 1 19:37:49 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 01 Feb 2002 17:32:46 -0800
Subject: Re: LM: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Your UPS is most likely a passive UPS....like APC
and similars. What you need is a dual conversion
UPS. These units continuously convert power line AC
to DC and back to AC. The passive units just sit there
and kick into battery if the input power line fails. There
is no regulation at all. I see input voltage varying between
103VAC to 117VAC. It is not constant 120VAC.

I use two maker's units....Powerware 9120 and Falcon
UPS Plus. These are very nice units. The 1KVA units
run about $500 or so. I use a mix of 1KVA and 1.5KVA
units. The nice thing about the Powerware units is that
they can be monitored over the LAN. The standard monitor
port for both is a COM port.

If you need contact info for either maker, pls let me know.
I am a very satisfied user of both product families.

gary g.



At 01:03 PM 2/1/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Feb 2 00:44:45 2002



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Sat, 2 Feb 2002 00:23:13 -0600
Subject: re: X-ray Microanalysis Reference Standards for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Krassi,

I think the Smithsonian Institute still supplies a limited amount of
mineral standard grains for microprobe analyses which are well suited
for TEM grain mounts. Some of them are big enough to make ion milled
samples. You well know the potential problems of shadowing the
detector in grain mount samples. The variety of minerals available
covers the rock-forming elements and then some. Let me know if they
have stopped this service.
Ciao for now,
Ken


From daemon Sat Feb 2 10:09:44 2002



From: David Burton :      dburton-at-nwlink.com
Date: Sat, 2 Feb 2002 07:59:16 -0800
Subject: Re: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Doug,

Because the lamp in mounted in this microscope with the socket up, large
amounts of heat are concentrated in the lampsocket. You are also drawing a
lot of current through the connections. Check the cables that connect the
power supply to the lamp, there are two, a long one and a short one. You
are looking for damage to the connectors, four of them. Changing these
cables or assuring that the contacts are clean and tight may solve the
problem.

Dave Burton



From daemon Sat Feb 2 11:10:30 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Sat, 2 Feb 2002 11:04:47 -0600
Subject: RE: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Don't know if anyone makes something commercially, but a lamp current
control based on feedback from sampling the light intensity should do it.

Woody

} -----Original Message-----
} From: Doug Cromey [mailto:Cromey-at-Arizona.edu]
} Sent: Friday, February 01, 2002 4:04 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: LM: lamp stability problems in time-lapse
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Listers,
}
} We have an Olympus IMT-2 set up for time lapse imaging using
} DIC. During
} our over-night exposures we find that the lamp does not
} provide consistent
} illumination, dimming occasionally. This makes for somewhat
} less than
} satisfactory movies. Is there a way we could make the lamp
} more stable?
}
} Details: 50W tungsten bulb, electrical power goes through a
} UPS before it
} enters the microscope, the lamp is typically at a setting of
} about 10/12 on
} the LCD, I will concede that the UPS is old & I'm unsure of
} the state of
} the batteries, although I'm inclined more to believe its the
} lamp or lamp
} housing.
}
} Thanks for your comments.
}
} Doug
}
} ....................................................................
} : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
} : Research Specialist, Principal University of Arizona :
} : (office: AHSC 4212A) P.O. Box 245044 :
} : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
} : (FAX: 520-626-2097) (email: Cromey-at-Arizona.edu) :
} :...................................................................:
} http://swehsc.pharmacy.arizona.edu/exppath/
} Home of: "Microscopy and Imaging Resources on the WWW"
}
}


From daemon Sat Feb 2 13:20:01 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Sat, 2 Feb 2002 13:09:50 -0600
Subject: Re: LM: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with Gary Gaugler in general but, if you only need to power a
50w light source, a good, adjustable, regulated power supply for this
alone (12 volts, 5 amps???) can be obtained for much less than $500.
Just run the lamp directly from this.

Jim P.



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
3D Microscopy of Living Cells Course, June 10 - 20, 2002, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2002


From daemon Sat Feb 2 14:37:27 2002



From: DrJohnRuss-at-aol.com
Date: Sat, 2 Feb 2002 15:30:09 EST
Subject: micron bars in Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There has been a recent thread discussing methods for using Photoshop to
place micron markers on micrographs. One thing that people seemed to want was
a method to directly use the (presumably known) image magnification to create
the final result. In response, I've created a plug-in for Photoshop (and
compatible programs) that does this. You enter the magnification of the image
(e.g., 1000x), the dpi with which it was acquired (e.g., 300 dpi for your
scanner, or the corresponding pixel spacing for your camera), and the length
of the bar you desire (in microns), and the program draws and labels the bar
in the lower right corner of the image using the selected foreground and
background colors. Using a Photoshop action, you can easily apply this
procedure to an entire folder of images.

This plug-in is freely downloadable for use on either Mac or Windows
computers from the ReindeerGraphics web site, at
{http://www.reindeergraphics.com/free.html#entermag} . It can be used on 8 and
16 bit grey scale images or 24 or 48 bit RGB color images. While it is
compatible with the Fovea Pro and Image Processing Tool Kit software, it does
not require them (but please do see what other kinds of processing and
measurement are available when you visit the web site).

If you have comments on the plug-in, or urgent needs for other features or
capabilities, please let me know.

John Russ
John_Russ-at-NCSU.edu



From daemon Sat Feb 2 16:31:24 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 02 Feb 2002 14:26:35 -0800
Subject: Re: LM: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Very good point, Jim. Yes indeed. For one little lamp,
a regulated DC supply will do nicely. I use these myself
as well. The only area of concern, or potential difficulty,
is mating the lamp itself to the supply. If I recall the
IMT-2 correctly, it has a separate lamp house with
interconnecting cable to the un-regulated AC supply
in the stand. She would either have to find a mating
connector to affix to the DC supply or whack off the
current connector and do whatever is necessary to
mate with the DC supply. Either way, it is definitely
cheaper than a dual conversion UPS.

Powerware makes lower VA rating units, down to
I think 500 VA. These units are a few hundred dollars.
I used to use DC supplies for 'scope lamps but with
all of the bad power problems last year here in California,
the dual conversion UPS solved many problems. I got
way larger units than I probably needed, only to ensure
long backup time.

A good source of supply for various DC supplies is
http://www.digikey.com

By all means, do not get a B&K 10Amp unit. It has
a soft start over-current feature which will trip with
a halogen lamp. When cold, they have low resistance
and have high starting current. I found out the hard
way about the B&K.

gary g.



At 11:09 AM 2/2/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Feb 2 21:00:32 2002



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 3 Feb 2002 00:29:36 -0600
Subject: Re: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kelly,

Try http://www.carnoy.org/ - you can download Carnoy program from this web
site. Carnoy is $15 shareware. It is written at Katholieke Universiteit
Leuven, in Netherlands http://www.kuleuven.ac.be .

First you have to calibrate the software for a particular instrument by
measuring known features of images taken with calibration sample at various
magnifications. Result of each measurement has to be written into the menu
along with the magnification value. Once set, use of the program becomes
simple. Just open the image file, then open the menu, choose magnification
value, length unit (from kilometer to nanometer- it will also work for
maps), number of units (length of the scale bar), and appearance of the
scale bar and characters (color, height, and location), and click OK.
Program will automatically superimpose scale bar and a legend on the image.
All these things can be set as default. Then only 3 clicks will put scale
bar on the image (open menu, choose mag., click OK).

You can undo the scale bar before the image file is saved, but once saved,
scale bar becomes part of the image.

Other features will allow you to define and count particles, measure
perimeters and densities, and convert image files into various formats back
and forth.

The only inconvenience which I encountered while using Carnoy software was
somewhat different response of the controls, as compared with expected
response of a standard Windows program. But technical support via e-mail was
adequate.

Disclaimer: SIA does not have any financial interest in Carnoy software. We
are just satisfied customers.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: {"kellymcg-at-seas.upenn.edu"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, January 31, 2002 2:53 PM



You can get power supplies that have sensing circuits built in them. I would
require some interface circuitry to be build to lock the power supply to the
light output.

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger

} From: "White, Woody N." {nwwhite-at-mcdermott.com}

: Don't know if anyone makes something commercially, but a lamp current
: control based on feedback from sampling the light intensity should do it.
:
: Woody
:
: } -----Original Message-----
: } From: Doug Cromey [mailto:Cromey-at-Arizona.edu]
: } Sent: Friday, February 01, 2002 4:04 PM
: } To: microscopy-at-sparc5.microscopy.com
: } Subject: LM: lamp stability problems in time-lapse
: }
: }
: } --------------------------------------------------------------
: } ----------
: } The Microscopy ListServer -- Sponsor: The Microscopy Society
: } of America
: } To Subscribe/Unsubscribe -- Send Email to
: } ListServer-at-MSA.Microscopy.Com
: } On-Line Help
: } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
: } --------------------------------------------------------------
: } ---------.
: }
: }
: } Listers,
: }
: } We have an Olympus IMT-2 set up for time lapse imaging using
: } DIC. During
: } our over-night exposures we find that the lamp does not
: } provide consistent
: } illumination, dimming occasionally. This makes for somewhat
: } less than
: } satisfactory movies. Is there a way we could make the lamp
: } more stable?
: }
: } Details: 50W tungsten bulb, electrical power goes through a
: } UPS before it
: } enters the microscope, the lamp is typically at a setting of
: } about 10/12 on
: } the LCD, I will concede that the UPS is old & I'm unsure of
: } the state of
: } the batteries, although I'm inclined more to believe its the
: } lamp or lamp
: } housing.
: }
: } Thanks for your comments.
: }
: } Doug
: }
: } ....................................................................
: } : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: } : Research Specialist, Principal University of Arizona :
: } : (office: AHSC 4212A) P.O. Box 245044 :
: } : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: } : (FAX: 520-626-2097) (email: Cromey-at-Arizona.edu) :
: } :...................................................................:
: } http://swehsc.pharmacy.arizona.edu/exppath/
: } Home of: "Microscopy and Imaging Resources on the WWW"
: }
: }
:
:



From daemon Sun Feb 3 03:29:56 2002



From: alan stone :      as-at-astonmet.com
Date: Sun, 03 Feb 2002 09:31:32 -0600
Subject: Re: LM: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After doing a bit of searching myself, I posted a request to a mailing list for The Gimp, asking how one might write a script to enter scale bars, using existing images at various magnifications. The Gimp (GNU Image Manipulation Program) is a free software project that works on GNU/Linux or Windoze, I think, as well as Mac OS/X, I think also. It is highly capable.

I wanted to show that there are other ways to do things than the old, hackneyed solutions.

Alan Davis

Begin forwarded message:




We had a similar problem with a metallograph. As it turned out, the dealer
included bulbs with oxidized leads. This led to arcing and subsequent
damage to the socket. We polished the contacting surfaces in the socket,
sputter coated them with gold, returned the old bulbs to the dealer and
purchased bulbs from another dealer with newer stock. The illumination is
stable and we haven't replaced a bulb in many years.



At 01:09 PM 2/2/2002 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Sun Feb 3 11:03:08 2002



From: Xianglin Li :      Xianglin_Li-at-student.uml.edu
Date: Sun, 03 Feb 2002 08:53:57 -0800
Subject: About some Parameters of III-V semiconductor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all

I am looking for some parameters of III-V semiconductors. If you can
help me, I will very appreciate for that.

1. Melting point of AlSb.
2. Hardness (on the Mohs scale) of GaN, AlSb, and InSb.

Thanks in advance!

Sincerely yours,
Xianglin

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Sun Feb 3 15:36:29 2002



From: gtg457a-at-prism.gatech.edu ()
Date: Sun, 3 Feb 2002 15:18:36 -0600
Subject: Ask-A-Microscopist: project pertaining to electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (gtg457a-at-prism.gatech.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
February 3, 2002 at 12:54:22
---------------------------------------------------------------------------

Email: gtg457a-at-prism.gatech.edu
Name: Christal Moore

Organization: Georgia Institute of Technology

Education: Undergraduate College

Location: Atlanta, GA USA

Question: My group in a class is working on a project pertaining to
electron microscopy. We do know that current imaging methods either
have lower spatial resolution or lack the temporal acquisition
capability. We are looking to for a new method or a new way of using
existing ones that would have spatial resolution sufficient for
depicting the smallest possible cellular structures, and temporal
resolution suitable for visualizing as many cellular processes as
possible. We have just begun researching electron microscopy and do
not know that much about it, such as to why are there are obstacles
with the spatial resolution and temporal resolution? Do you know of
any new methods you could share with the group, or perhaps a good
staring point for us to being searching? Also, is it possible to
view cellular processes? What are the steps for doing so? Do you
suggest any other experts to contact? Thank you for your time.

---------------------------------------------------------------------------


From daemon Sun Feb 3 18:37:01 2002



From: pcy :      pcy-at-usc.edu
Date: Sun, 3 Feb 2002 16:29:33 -0800 (PST)
Subject: Propidium iodide staining 4 confocal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are hoping to stain the nuclei of polychaete worm larvae with
propidium iodide. The problem is that due to small sample size, it's kind
of a "one-shot" deal and I have no protocol to refer to for concentrations
of stain to use. I've searched the web and found protocols saying
everything between 0.5mg/ml for sea urchin larvae to 1 micromolar for
Xenopus embryos. Should we err on the side of excess and go with the
really high concentration?

Background prep info:
fixed in 4% paraformaldehyde in sea water
labeled with anti-tubulin FITC-conj. antibody

The info sheets from Molecular Probes also recommends RNAse pretreatment.
Do people have input on this?

Thanks,
Pauline Yu
Manahan Research Lab
http://www.usc.edu/manahanlab



From daemon Sun Feb 3 22:28:08 2002



From: Young W. Kim :      ywkim-at-gong.snu.ac.kr
Date: Mon, 4 Feb 2002 00:57:57 -0600
Subject: About some Parameters of III-V semiconductor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


After doing a bit of searching myself, I posted a request to a mailing list for
The Gimp, asking how one might write a script to enter scale bars, using
existing images at various magnifications. The Gimp (GNU Image Manipulation
Program) is a free software project that works on GNU/Linux or Windoze, I think,
as well as Mac OS/X, I think also. It is highly capable.

I wanted to show that there are other ways to do things than the old, hackneyed
solutions.

Alan Davis

Begin forwarded message:



?Xianglin,

Melting temperature of AlSb = 1338K (J. Phys. Chem. Solids 36 (1975) p.931)
microhardness of AlSb = 413 kg /mm2 or 359 kg/mm2(From
Landolt-Bornstein, vol 17, semiconductors, Springer 1982)
Good luck.

Young W. Kim, Ph.D.
Research Professor
School of Materials Science and Engineering
Seoul National University
Kwanak-ku Shinlim-dong San 56-1
Seoul, Republic of Korea 151-744

Tel) + 82-2-880-7977
E-mail) ywkim-at-gong.snu.ac.kr

-----Original Message-----
} From: Xianglin Li [mailto:Xianglin_Li-at-student.uml.edu]
Sent: Monday, February 04, 2002 1:54 AM
To: Microscopy-at-sparc5.microscopy.com


Hi, all

I am looking for some parameters of III-V semiconductors. If you can
help me, I will very appreciate for that.

1. Melting point of AlSb.
2. Hardness (on the Mohs scale) of GaN, AlSb, and InSb.

Thanks in advance!

Sincerely yours,
Xianglin

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411


From daemon Mon Feb 4 01:11:09 2002



From: pooley-at-tidewater.net ()
Date: Mon, 4 Feb 2002 00:57:25 -0600
Subject: Ask-A-Microscopist:SEM digitizer?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (pooley-at-tidewater.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
February 3, 2002 at 19:28:15
---------------------------------------------------------------------------

Email: pooley-at-tidewater.net
Name: Alan S. Pooley, PhD

Organization: retired, volunteer at Umaine marine center

Education: Graduate College

Location: Newcastle & Walpole Maine

Question: Is there a good, relatively cheap digitizer (at least 1024
or 2048 square pixels) for the Zeiss DSM940A SEM? Company name or
web site info sought please

---------------------------------------------------------------------------


From daemon Mon Feb 4 02:13:35 2002



From: Bharesh_Mandalia-at-gillette.com
Date: Mon, 4 Feb 2002 08:04:54 +0000
Subject: Convert .VTI to .BMP abd vice versa

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Dear All,

I was recently sent some images for image analysis. Unfortunately they in
.VTI file format. Is there any software which can convert to .BMP or .TIF
format?

Many thanks,

Bharesh Mandalia



From daemon Mon Feb 4 06:07:01 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Mon, 04 Feb 2002 06:58:41 -0500
Subject: Re: solubilities of fixatives

Contents Retrieved from Microscopy Listserver Archives
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Rosemary,
Sometimes that information can be found in the MSDS (sorry, Material
Data Safety Sheet - required in the US) for the chemical. VWR has them
on line (probably others do, also).

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Rosemary White wrote:

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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Feb 4 06:14:04 2002



From: microscope-at-tin.it
Date: Mon, 4 Feb 2002 13:09:00 CET
Subject: Microphotography

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Torino 04 January 2002
(Italy)

Dear microscopists,

Does anyone have a triocular LOMO head attachment MFN-11 on the microscope?
I’m a beginner and I’d like to talk about microphotography concerning:
Kind of B/W film
Focusing of specimen on the film plane inside the camera
Filters
Optical microscope setup
Exposure film time

Thank you. Kind Regards,

Massimo




From daemon Mon Feb 4 06:41:40 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 4 Feb 2002 12:35:42 +0000 (GMT Standard Time)
Subject: TEM of bacteriophages

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I have some qusetions about EM of bacteriophages.
They are double-stranded DNA phages for the plant
pathogenic bacterium Pseudomonas syringae.

1. Negative staining. Could someone suggest a good stain
and protocol for this type of bacteriophage?

2. Embedding in resin. The bacteriophages will be attached
to bacteria on an agar plate. Can anyone suggest a good
way to handle them. I suspect if I just cut out a piece of
agar I could lose a lot of sample during dehydration.

Thanks in advance

Dave

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Feb 4 06:55:25 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Mon, 4 Feb 2002 07:57:15 -0500
Subject: Re: Convert .VTI to .BMP and vice versa

Contents Retrieved from Microscopy Listserver Archives
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I do not know any software that can do this, but you could try ctrl+print
screen to copy screen and ctrl+insert to paste the file in windows paint
program or any other program. After you insert the image crop it and save in
BMP format.
If you have any problems e-mail me.

Pavel Lozovyy
Argo-Tech SEM lab
atcsem-at-earthlink.net






From daemon Mon Feb 4 08:36:33 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Mon, 4 Feb 2002 10:59:09 -0330
Subject: RE: micron bars in Photoshop

Contents Retrieved from Microscopy Listserver Archives
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John Russ writes ...

} ...
} ... I've created a plug-in for Photoshop (and compatible
} programs) that does this. You enter the magnification
} of the image (e.g., 1000x), the dpi with which it was acquired
} ...
}
} This plug-in is freely downloadable ... at
} {http://www.reindeergraphics.com/free.html#entermag} . ...

Thanks John. I wonder however ... what I had noticed fo a variety of
image acquisition systems, that a specific constant was needed. That is,
even for a specific SEM which indicated the magnification, I had to use a
different magnification constant depending on the acquisition software
(e.g., JEOL's own or alternative Oxford). How would your plugin address
this? (I can tell you one one method, vs the other, acquired only a portion
of the other ... e.g., the Oxford acquired a square image from the center of
the rectangular JEOL image)

My own method was to create a printable spreadsheet for my SEM users,
which (depending on acquisition method) would indicate the "length of a
selection box" for a given magnification. This also allowed for user
preferred fonts, micron-bar placement, and style (e.g., simple line,
outlined box, black on white, W/B, or color). It certainly wasn't
automatic, but it was the only way to accommodate users' presentation
preferences.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland



From daemon Mon Feb 4 08:48:40 2002



From: Heeschen, Bill (WA) :      WAHEESCHEN-at-dow.com
Date: Mon, 4 Feb 2002 09:43:20 -0500
Subject: Re: lamp stability problems in time-lapse

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I've dealt with this problem (supply voltage fluctuation of a few volts) by
using a constant voltage transformer (SOLA). The problem with the UPS it
that it tracks the supply voltage. The only time the UPS' regulated output
kicks in is when the supply voltage disappears. My biggest complaint with
the Sola transformer is that it is mechanically noisy, so it gets annoying
to be in the area for a long time. They also put out a little electrical
noise, but if you can physically isolate the transformer and put it on a
separate circuit from your analytical instrumentation, then that shouldn't
be much of a problem. Most instrumentation has decent filtering on the
power line, anyhow.

For critical work, the light output feedback is the best, assuming you can
get a sensor mounted somewhere.

Bill
William A. Heeschen, Ph.D.
The Dow Chemical Company
Microscopy, Digital Imaging
1897 Bldg, E-84 / 2040 Bldg, 1330
waheeschen-at-dow.com
voice: 989-636-4005 fax: 989-638-6443


-----Original Message-----
} From: Gordon Couger [mailto:gcouger-at-couger.com]
Sent: Sunday, February 03, 2002 1:30 AM
To: microscopy-at-sparc5.microscopy.com



You can get power supplies that have sensing circuits built in them. I would
require some interface circuitry to be build to lock the power supply to the
light output.

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger

} From: "White, Woody N." {nwwhite-at-mcdermott.com}

: Don't know if anyone makes something commercially, but a lamp current
: control based on feedback from sampling the light intensity should do it.
:
: Woody
:
: } -----Original Message-----
: } From: Doug Cromey [mailto:Cromey-at-Arizona.edu]
: } Sent: Friday, February 01, 2002 4:04 PM
: } To: microscopy-at-sparc5.microscopy.com
: } Subject: LM: lamp stability problems in time-lapse
: }
: }
: } --------------------------------------------------------------
: } ----------
: } The Microscopy ListServer -- Sponsor: The Microscopy Society
: } of America
: } To Subscribe/Unsubscribe -- Send Email to
: } ListServer-at-MSA.Microscopy.Com
: } On-Line Help
: } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
: } --------------------------------------------------------------
: } ---------.
: }
: }
: } Listers,
: }
: } We have an Olympus IMT-2 set up for time lapse imaging using
: } DIC. During
: } our over-night exposures we find that the lamp does not
: } provide consistent
: } illumination, dimming occasionally. This makes for somewhat
: } less than
: } satisfactory movies. Is there a way we could make the lamp
: } more stable?
: }
: } Details: 50W tungsten bulb, electrical power goes through a
: } UPS before it
: } enters the microscope, the lamp is typically at a setting of
: } about 10/12 on
: } the LCD, I will concede that the UPS is old & I'm unsure of
: } the state of
: } the batteries, although I'm inclined more to believe its the
: } lamp or lamp
: } housing.
: }
: } Thanks for your comments.
: }
: } Doug
: }
: } ....................................................................
: } : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: } : Research Specialist, Principal University of Arizona :
: } : (office: AHSC 4212A) P.O. Box 245044 :
: } : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: } : (FAX: 520-626-2097) (email: Cromey-at-Arizona.edu) :
: } :...................................................................:
: } http://swehsc.pharmacy.arizona.edu/exppath/
: } Home of: "Microscopy and Imaging Resources on the WWW"
: }
: }
:
:



From daemon Mon Feb 4 09:20:14 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 4 Feb 2002 09:14:22 -0600
Subject: RE: micron bars in Photoshop

Contents Retrieved from Microscopy Listserver Archives
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I have set of images with different magnifications
in Photoshop .psd format with additional layers
with micron bars on them. Now I can just drag and drop
layer with micron bar on new images with the same magnification
after I crop them. It works fine for me since number of
images in publications and presentations anyway is quite limited.
Of course, it is possible to use Photoshop's "automate" to
place micron bars on many images with the same magnification.

To create image with micron bar for copying:
1. create new layer
2. draw micron bar with the same length as original bar.
3. using Type Tool type N Microns (or millimeters or
whatever else), new (third) layer will be created automatically
4. make background layer invisible
5. click on layer-} merge visible
6. make background visible again
7. save in .psd format with name "Magnification M"

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy




From daemon Mon Feb 4 10:08:41 2002



From: Roberto Garcia :      rgarcia-at-unity.ncsu.edu
Date: Mon, 4 Feb 2002 11:00:43 -0500
Subject: Scheduling Software

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I know that this has been asked before but I just want to see if there has
been any updated software. I would like to know what is available out there
for online scheduling of equipment. Thanks.
______________________________
Roberto Garcia
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm




From daemon Mon Feb 4 11:51:53 2002



From: Russell McConnell :      russell.mcconnell-at-tufts.edu
Date: Mon, 04 Feb 2002 12:40:52 -0500
Subject: Re: Ask-A-Microscopist: project pertaining to electron microscopy

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Dear Christal,
First, let me wish your group the best of luck with this project, it is
a difficult but worthwhile undertaking. The problem with obtaining
spatial and temporal resolution is a complex one. To start I will point
out one of the fundamentals of microscopy, the Abbe equation: (I would
suggest visiting this web site for a better explanation:
http://lsvl.la.asu.edu/bio598L/notes/lightlenses/ )
resolution = (0.612 x l) / (n sin a)

l = (wavelength of light)
n = refractive index of medium between the objective lens and the
specimen.
a = the aperture angle, which is half the angle of the cone of light
from the specimen accepted by the front lens objective. This is also
called the half aperture angle.
(n sin a) is the 'numerical aperture' of a lens, and is usually printed
on the side of objectives for light microscopes.

What is important to note is that resolution is a function of
essentially two things, your lens and the light you are using. More
specifically, the wavelength of the light is related to resolution in
such a way that shorter wavelength = better resolution. Electron
microscopes achieve their remarkable spatial resolution through the use
of electrons as the 'light'. The wavelengths of visible light are in
the range of 400-700 nm; electrons have a considerably shorter
wavelength, about 0.005nm (recall that matter behaves as a particle and
also as a wave). It is because of this shorter wavelength that electron
microscopes have such excellent spatial resolution.
So, electron microscopes have excellent spatial resolution; why don't
they have temporal resolution as well? Temporal resolution is the
ability to see change over time. To see a cellular process change over
time, you must be looking at a live cell. Unfortunately, it is not
possible to do this with an electron microscope for a number of reasons
(there are probably others, but these are the most obvious, at least to
me). The first has to do with the fact that electrons are very easily
scattered when they pass into/through matter; electrons are so easily
scattered that the tube of electron microscopes must be pumped down to a
vacuum so that the air in the column doesn't scatter the electron beam!
If electrons have a hard time traveling through air, one can imagine
that the relatively denser matter of a biological specimen would be
nearly impenetrable. To make it possible for electrons to get through a
specimen, you have to cut the specimen into very thin sections. A
typical eukaryotic cell might be on the order of 10-15 microns thick;
most sections for transmission electron microscopy are around 0.1
microns thick. After sectioning a specimen, electrons will pass through
it. The challenge then becomes being able to distinguish cellular
structures. A professor of mine once compared this to looking for
chunks of clear Jell-O in a swimming pool. The problem is that
electrons (and light in general) pass through most cell components in
the same way. To get contrast you have to stain specimens with an
electron dense material, usually a heavy metal stain such as Osmium
tetroxide (OsO4). So this gives us three major reasons why living cells
hate electron microscopy:
1. specimens must be placed in a vacuum while they are being viewed
2. you have to cut the specimen into very thin sections
3. electron dense stains (such as OsO4) are usually highly toxic
None of these conditions are compatible with living cells. To make
matters even worse, it is necessary to imbed specimens in a plastic
resin for them to hold their shape during sectioning (otherwise it's a
bit like slicing a tomato with a butter knife).
For information on microscopic techniques currently in use I would
recommend the following web sites:
University of Arizona's web site list
http://swehsc.pharmacy.arizona.edu/exppath/micro/index.html
Lance Ladic's site on confocal microscopy
http://www.cs.ubc.ca/spider/ladic/overview.html
You may also want to visit the home pages for the major microscope
manufacturers, such as Zeiss, Leica, Olympus, Nikon, BioRad, etc.
Again, best of luck to you.
--
Russell McConnell
Confocal Imaging Facility Technician
Department of Neuroscience
Tufts University School of Medicine
M&V Building room #137
136 Harrison Ave.
Boston, MA 02111
Tel. (617) 636-3795

gtg457a-at-prism.gatech.edu wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (gtg457a-at-prism.gatech.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
} February 3, 2002 at 12:54:22
} ---------------------------------------------------------------------------
}
} Email: gtg457a-at-prism.gatech.edu
} Name: Christal Moore
}
} Organization: Georgia Institute of Technology
}
} Education: Undergraduate College
}
} Location: Atlanta, GA USA
}
} Question: My group in a class is working on a project pertaining to
} electron microscopy. We do know that current imaging methods either
} have lower spatial resolution or lack the temporal acquisition
} capability. We are looking to for a new method or a new way of using
} existing ones that would have spatial resolution sufficient for
} depicting the smallest possible cellular structures, and temporal
} resolution suitable for visualizing as many cellular processes as
} possible. We have just begun researching electron microscopy and do
} not know that much about it, such as to why are there are obstacles
} with the spatial resolution and temporal resolution? Do you know of
} any new methods you could share with the group, or perhaps a good
} staring point for us to being searching? Also, is it possible to
} view cellular processes? What are the steps for doing so? Do you
} suggest any other experts to contact? Thank you for your time.
}
} ---------------------------------------------------------------------------


From daemon Mon Feb 4 12:14:35 2002



From: Curtis Olson :      COlson-at-scpglobal.com
Date: Mon, 04 Feb 2002 11:05:37 -0700
Subject: Cross Section Thank you

Contents Retrieved from Microscopy Listserver Archives
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Thank you who responded to my request for help with SEM semiconductor cross sections. Many responses with excellent information.
Curtis Olson



From daemon Mon Feb 4 12:21:17 2002



From: Curtis Olson :      COlson-at-scpglobal.com
Date: Mon, 04 Feb 2002 11:12:43 -0700
Subject: Environmental Noise Impacting JEOL 848 SEM

Contents Retrieved from Microscopy Listserver Archives
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I operate a JEOL 848 SEM but am limited to less than 30,000x due to at least two frequencies of external noise. The service engineers from JEOL have determined the noise is not due to the SEM. I will be conducting as much of a room survey as possible later in February but I am hoping to obtain some insight into common testing formats, noise sources, and solutions. From what I have been able to learn so far, this noise reduction is something of an art rather than science. Thank you in advance from this microscopist with much to learn.

Curtis Olson



From daemon Mon Feb 4 13:35:27 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 04 Feb 2002 11:26:57 -0800
Subject: RE: lamp stability problems in time-lapse

Contents Retrieved from Microscopy Listserver Archives
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Sola magnetic resonance transformers are good for this
application. However, they are not cheap--especially
as their VA rating increases. And yes, they get noisy.

For critical work, I suggest either a regulated DC supply
or a dual conversion UPS. The dual conversion UPS
never "kicks in" but rather is always producing regulated
(frequency and voltage) AC from DC. The DC is either
that from rectified line voltage or from the UPS batteries.

gary g.





At 06:43 AM 2/4/2002 , you wrote:
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From daemon Mon Feb 4 15:30:29 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 4 Feb 2002 11:21:55 -1000 (HST)
Subject: Nikon Coolpix vs Olympus C-3030 et al.

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Hi, All-

Have any of you compared the Nikon Coolpix 995 (or earlier) with the
Olympus C-4040 (or earlier) mounted on a light microscope? They are both
comsumer-level digital cameras with about the same technology, and so I'm
wondering if there is any reason to choose one over the other. The Olympus
has better features on paper, so I'm hoping to hear from someone who uses
one. This will be used to supplement the Magnafire SP I'm proposing to
buy, since I need a camera that will fit into the eyepiece of our
confocal.

Mahalo!
Tina



****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon Feb 4 16:46:57 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Mon, 4 Feb 2002 17:21:56 -0500
Subject: Protein dimers and chains

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Dear Listers:

I have been trying to image with negative stain, protein chains and protein
dimers with 2.0% Uranyl Acetate. It seems when I pipet onto the grid,
various dilutions (10-100x) of the buffered protein dimer sample, I get alot
of protein chain formation. I want the dimer form to stay in that
configuration-- not link up into chains. I am photographing at 100,000
using 75 kv on a traditional Hitachi 7100 TEM.

Does anyone out there work with these types of specimen? Is there a special
way to prep the grid, dry the grid, etc.? Should I use some other type of
grid besides the formvar/carbon coated copper grid? Should I sonicate the
specimen before I place the sample on the grid?

Thanks for any help you can provide.

Karen Bentley, M.S.
(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954



From daemon Mon Feb 4 17:27:39 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Mon, 4 Feb 2002 18:22:15 -0500
Subject: Environmental Noise Impacting JEOL 848 SEM

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Curtis,

Don't be too quick to accept the service engineers claim that the SEM is
not
the source of noise in the image. When they can't easily figure out what
the
problem is, it is too easy for them to blame the "fields," because it is
something
not well understood by most people. I went through all of the field
service
people, the service people at the JEOL headquarters in Massachusetts, and
an "expert" from Japan. The expert's final conclusion was that the problem
was
caused by the fields in the room, and not our new SEM. A while later, we
moved
the SEM some miles down the road to a different building. We had JEOL
check
the room before moving in, and they blessed the room. When we had the
exact
same problem, I had a discussion with the people in Massachusetts.
Eventually,
we had a repaired system that provided excellent images. This all took way
too
long to resolve. The only company that I am aware of having intimate
knowledge
of their systems "noise" was Amray. They used a spectrum analyzer on the
system, and identified every source of every frequency.

Good luck! Your problem will, most likely, not be this difficult.
Darrell

"Curtis Olson" {COlson-at-scpglobal.com} on 02/04/2002 01:12:43 PM

To: {Microscopy-at-sparc5.microscopy.com}
cc:


I operate a JEOL 848 SEM but am limited to less than 30,000x due to at
least two frequencies of external noise. The service engineers from JEOL
have determined the noise is not due to the SEM. I will be conducting as
much of a room survey as possible later in February but I am hoping to
obtain some insight into common testing formats, noise sources, and
solutions. From what I have been able to learn so far, this noise reduction
is something of an art rather than science. Thank you in advance from this
microscopist with much to learn.

Curtis Olson







From daemon Mon Feb 4 17:58:29 2002



From: curari-at-asu.edu
Date: Mon, 04 Feb 2002 16:50:46 -0700 (MST)
Subject: ISI SX-40 help

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I am a student at Scottsdale Community College. I am working with an
ISI SX-40 Scanning Electron Microscope. It has an old polaroid camera that
snaps a picture of a CRT image. I am looking for options(and donated
equipment) to put these images on to a PC, the big problem is I am on a
very tight budget($800) so I am very willing to look for used equipment
(card or camera or info) . This machine is old and did not
work at all. Over the past 8 months or so I have rebuilt the diffusion pump,
roughing pump, new seals, column cleaning, and learned basic operation by
myself with this apparatus. And I have learned a lot from following this list
server, so I wanted to send out a big thanx to all the listers. Thank you in
advance for your time and consideration. Any and all input is appreciated

Wil Kunkel
Student Extraordinaire

This email was sent with 100.00% recycled electrons.



From daemon Mon Feb 4 19:07:49 2002



From: Bob :      bobrobs-at-earthlink.net
Date: Mon, 04 Feb 2002 10:23:18 -0700
Subject: Re: Environmental Noise Impacting JEOL 848 SEM

Contents Retrieved from Microscopy Listserver Archives
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Curtis,

I would start with a close inspection of the power supplied to the SEM.
Although measuring for stray fields and floor
vibration may be in order, a good place to begin is with the A/C power
and the potential for ground loops if wired common
or shared with other equipment. The ground terminal into the microscope
main power supply can often be a good source of noise.

On a positive note, if this noise is a problem as low as 30KX it should
be somewhat easier to find.

Regards,

Bob Roberts
EM Lab Services, Inc.
Tempe, Arizona 85284


Curtis Olson wrote:

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From daemon Mon Feb 4 20:40:50 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 4 Feb 2002 20:33:15 -0600
Subject: Second announcement: Seventh Annual UBC Live-Cell course

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Announcing the Seventh Annual INTERNATIONAL 11-Day Short Course on

3D Microscopy of Living Cells, June 10 - 20, 2002

Sixth, Post-course Workshop on, 3D Image Processing, June 22 - 24, 2002

Organized by Prof. James Pawley, University of Wisconsin-Madison

in association with the, UBC Brain Research Centre, Prof. Max
Cynader, Director.
University of British Columbia, Vancouver, BC, Canada

(CORRECTION!! some early brochures were sent out with an incorrect URL.
Course info can be found at: ht tp://www.3dcourse.ubc.ca)

THE PURPOSE OF THE COURSE

Modern methods of 3D light microscopy promise a revolutionary
improvement in our ability to view living cells. To help convert
this promise to reality for a wider selection of biological
scientists, the organizers have designed an intensive eleven-day
residential course concentrating on all aspects of the 3D Microscopy
of Living Cells. Sponsored by the Brain Research Centre at the
University of British Columbia, it will be held in June of 2002. The
course includes 4 days on 2D techniques, 5 days of 3D techniques and
2 days on 3D measurement and display. It includes everything from
basic microscopy to confocal and multiphoton microscopy. A half-day
Pre-course is offered for those wishing to brush up on (very!) basic
optics.


INTERNATIONAL FACULTY
o Stephen Adams University of California-SD
o Dan Axelrod University of Michigan
o Mark Cannell University of Auckland, NZ
o Rainer Duden Cambridge Institute for Medical Research, UK
o Ping Chin Cheng SUNY, Buffalo
o Stefan Hell Max Planck Institute, Goettingen
o Alan Hibbs BioCon, Melbourne, Australia
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andres Kriete Tissue Informatics, Pittsburgh
o Glen MacDonald Virginia Bloedel Hearing Inst, WA
o Irina Majoul Max Planck Institute, Goettingen
o Felix Margadant University of Sydney, AU
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Technology
o Badri Roysam Rensselaer Polytechnic Institute, NY
o Lee Tierney Eppendorf Scientific,Albequerque, NM
o Michael Weis Agriculture Canada


APPLICATIONS
Applicants will submit an application to assess knowledge level and
field of interest. Enrollment will be limited to 24 - 32
participants (depending on equipment availability). Selection will
be made on the basis of background and perceived need. Those with
little previous LM experience will be provided with basic texts to
read before the course begins, and should take the Pre-course.

Application forms and other course information from this and past
years can be downloaded from the WWW site at

h ttp://www.3dcourse.ubc.ca/home.html

or obtained from:

Prof. James Pawley,
Zoology Department.,
1117 W. Johnson Drive,
Madison, WI.
Phone: 608-263-3147 fax. 608-265-5315
Email: jbpawley-at-facstaff.wisc.edu

IMPORTANT DATES
Applications must be received by Mar. 15, 2002
Deposit due Apr. 15, 2002
Registration 5:00 - 7:00 pm Sunday, June 9, 2002
Intro. Lecture 7:00 PM, Sunday, June 9, 2002
Last class ends with lunch, Thursday, June 20, 2002
3D IP Workshop Saturday, June 22-24, 2002
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Mon Feb 4 21:12:24 2002



From: jhardy-at-coh.org ()
Date: Mon, 4 Feb 2002 15:53:52 -0600
Subject: Ask-A-Microscopist: Image Supercharger for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jhardy-at-coh.org) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
February 4, 2002 at 15:28:55
---------------------------------------------------------------------------

Email: jhardy-at-coh.org
Name: John Hardy

Organization: City of Hope Medical Center

Education: Graduate College

Location: Duarte, California

Question: Does anyone have experience with, or comments about the
"Image Supercharger Upgrade" by Ascend Technical Sales? It would be
an add-on image processor for our "mature"(i.e. 1984) Philips 505
SEM. Please contact me off line at:
jhardy-at-coh.org
Thank you in advance
John Hardy
City of Hope Medical Center
Duarte, CA
(626)301-8265

---------------------------------------------------------------------------


From daemon Mon Feb 4 21:13:31 2002



From: zaluzec-at-microscopy.com
Date: Mon, 4 Feb 2002 15:53:16 -0600
Subject: Re: Ask-A-Microscopist:Help LM Info for a Middle School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



} Email: ever4us-at-comcast.com
} Name: Denise Everett
}
} Organization: Pitman Middle School
}
} Education: 6-8th Grade Middle School
}
} Location: Pitman, NJ 08071
}
} Question: I've recently become the science coordinator of our school
} but my science background is in enzymology so I don't have alot of
} experience with microscopy. We are looking to buy some new scopes
} and in the process I've been looking at our old ones. The 400x
} magnification is pretty unusable on these. Is that supposed to be oil
} immersion use only?
} These lenses are pretty dirty and have probably not been maintained.
} The top objective does not come out for cleaning as far as I can see.
} Do I just need to send these to a technician?
}
} ---------------------------------------------------------------------------
Denise -

I'm glad that you've asked for help; we're here to provide it.
There are several topics here. First, new microscopes. Let's assume that
the scopes that you have are salvageable. Since you're in a middle school,
PLEASE consider purchasing "dissecting" rather than compound scopes like
the ones that you have. A lot of introductory microscopy for your age
group is observstion of thick specimens at lower magnifications; looking at
large insects, flowers, shells, etc. So having a mix of types will greatly
expand your capabilities. You'll find a detailed discussion of selection
criteria on Project MICRO's website (URL below). I suggest 20x monocular
dissecting scopes, wich will cost you around $75 each; sources are listed
on the MICRO site and you can find an example online at
www.microscopeworld.com.
Your dirt diagnosis is probably accurate. It would be best if you
learn to clean the scopes yourself; you'll then know how to keep them that
way. The New York Microscopical Society has members and meeting rooms in
New Jersey, and one of their members may be available to show you what to
do; I'm copying this Email to Jean Portell {JeanDP-at-aol.com} , one of their
officers. I also can provide you with detailed cleaning instructions for
teachers, written by a MSA member. 400x is "high dry" - oil immersion is
1000x and inappropriate for middle school.
While you're visiting the MICRO website, don't miss MSA's middle
school manual, "Microscopic Explorations"; it's an excellent introduction
to scientific observation and inquiry, written by the science educators at
the Lawrence Hall of Science. If you want a reference book for your own
use, here's another listing from the MICRO bibliography:

Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy; it's unequalled as a
basic reference for beginners. Almost half of the book is devoted to
simple preparation methods for biological specimens and descriptions (with
good illustrations) of commonly encountered organisms. Adult. RECOMMENDED

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html


From daemon Mon Feb 4 22:32:43 2002



From: Pradyumna Prabhumirashi :      p-prabhumirashi-at-northwestern.edu
Date: Mon, 4 Feb 2002 22:25:12 -0600
Subject: EELS Raw Data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello,

I need some help in converting some raw EELS data into formats readable
by either EL/P or digital micrograph. The data I have gathered is in
.ZAL (Zaluzek) format. The data looks very similar to the .TAD format.
Let me know if there is a program that reads .ZAL format and converts it
to .TAD or even two column text. Perhaps Nestor would be able to help in
this regard, right? :).
Thanks in advance,

Prad

Pradyumna Prabhumirashi
Dept. of Materials Science
Northwestern University
Phone: (847)491-7798
Fax : (847)491-7820




From daemon Mon Feb 4 22:47:47 2002



From: Beauregard :      beaurega-at-westol.com
Date: Mon, 04 Feb 2002 18:37:51 -0500
Subject: Straight parallel micron bars in Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
To create an image with a labeled micron bar in 20-30 seconds:
1. Make the info tab totally visible as a separate window on the right
side of PS.
2. Crop your image. I assume you are calibrated in cm.
3. Click on the line drawing tool.
4. In the lower left corner, click where you want the marker to start.
5. Drag to the right slightly, then hold down the shift key for a perfect
straight line. (Use the option tab to increase the line width (5-15 pixels).)
6. Drag to the right until the D: in the INFO window that shows the length
you need on the final printed image
You should take into account how you might have changed the DPI values.
For example, if you scanned a neg. at 600 DPI and cut it to 200 DPI, then a
negative of 10,000X will be about 30,000X on the print and the line length
for 1µm should be D: 3.00 CM in INFO. This varies a bit with your
printer. Use scanned graph paper to calibrate your DPI to exactly 3x. I
use 196 DPI on a HP1200TN to get 3X, after scanning at 600 DPI.
7. CAREFULLY release the mouse button, then the shift key and you have
your perfect line.
8. Click on the type tool and click at the position you want the text
above the marker. I use centered, 15 point, crisp, black ink, ariel MT,
bold, etc.
9. Type in using 15 points the length of the line; 1µm, 0.1µm, 0.3 µms, etc.
10.. Highlight the "1µm" text and copy it as a template to the clipboard
for the next image. Click OK.
11. Continue to the next image.

µ = press and hold down the ALT key,
on the NUMSPAD on the right of the keyboard,
type 0181,
release the ALT key.
µ appears in ANY windows program.
Try:
http://www.dtp-aus.com/ext_set.htm
for more extended characters and a nice table you can print.
Å, ², ³,©, ®, ±, £, ö, •, 0176°

Paul Beauregard
Senior Research Associate
PPG Industries
440 College Park Drive
Monroeville, PA 15146



From daemon Tue Feb 5 00:07:19 2002



From: Mark YEADON :      m-yeadon-at-imre.org.sg
Date: Tue, 5 Feb 2002 14:03:52 +0800
Subject: 300kV LaB6 TEM for polymer analysis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

We are in process of buying a TEM for our Science Faculty; it would be used
by physicists and materials scientists, and also chemists (inorganic and
organic samples).

We have a 300kV FEG machine with ultra hi res polepiece (restricted tilt).
We do polymers and inorganics, no problem. We now need a LaB6 machine
(budget constraint) with high tilt to be more of a Workhorse Machine.

For the sake of resolution and penetration, we are favoring a 300kV
analytical TEM with ~2.1A resolution, and around 40 degrees tilt. This will
be perfect for the physical scientists.

HOWEVER: The chemists are worried that they will have trouble looking at
their polymer samples in a 300kV LaB6. Can someone help to support me (or
correct me) in my thesis that this machine will be able to support them just
as well as a 200kV machine? (I know eg that the HT can be reduced to 100 or
200kV, but the chemists are still skeptical - they have no prior TEM
experience).

If there is someone with a 300kV LaB6 TEM running polymers, I would be very
glad to hear from you! Any references to your papers that would show
polymers imaged in a 300kV LaB6 machine would be most welcome (esp any soft
copies that I can circulate to our committee).

Thanks to all in advance,

Mark

%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119260
http://www.matsci.nus.edu.sg/STAFF/Mark.html

TEL: (+65) 874 8591
FAX: (+65) 872 0785
Email: m-yeadon-at-imre.org.sg


From daemon Tue Feb 5 02:26:25 2002



From: Peter Heimann :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Tue, 05 Feb 2002 09:16:58 +0100
Subject: self coating of EM viewing screen (looking for help/recipe)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

who has any personal experience in self coating of an EM viewing
screen and can send me his advice?

a few years ago there was a reply to a similar topic by {underline} Bill Tivol {/underline} ,

however his e-mail adress must have changed and he can`t be

reached by the old one.

peter heimann (Bielefeld / Germany) {color} {param} 0100,0100,0100 {/param}

{nofill}
**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************


From daemon Tue Feb 5 09:37:59 2002



From: David_R_Stadden-at-armstrong.com
Date: Tue, 5 Feb 2002 10:28:55 -0500
Subject: NIST-traceable stage micrometer?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I've been asked if the stage micrometers I use for calibrating our optical
scopes are NIST-traceable. Is there such a thing and, if not, what would
satisfy requirements for ISO certification in the area of metrology?

Thanks!

Dave Stadden
Research Scientist
Armstrong World Industries, Inc.
Lancaster, PA




From daemon Tue Feb 5 10:46:05 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 5 Feb 2002 08:35:38 -0800
Subject: Microscope philately

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bob Bloodgood, a cell biologist at the University of Virginia, has posted
nice images of light microscopes on postage stamps on the web at
http://www.med.virginia.edu/med-ed/cell/stamps/index.html

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Feb 5 11:31:27 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Tue, 5 Feb 2002 11:22:43 -0600
Subject: Re: Environmental Noise Impacting JEOL 848 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might also have a look at

Pawley, J.B. Strategy for Locating and Eliminating Sources of Mains
Frequency Stray Magnetic Fields. Scanning 7:43-46 (1985).

Pawley, J.B. Use of Pseudo-Stereo Techniques to Detect Stray Field in
the SEM. Scanning 9-3:134-136 (1987).

The matter of ground loops, and stray fields from bad house wiring
are a bit complex to discuss in emails. Please excuse the
self-advertisement.

Jim P.




} Curtis,
}
} I would start with a close inspection of the power supplied to the
} SEM. Although measuring for stray fields and floor
} vibration may be in order, a good place to begin is with the A/C
} power and the potential for ground loops if wired common
} or shared with other equipment. The ground terminal into the
} microscope main power supply can often be a good source of noise.
}
} On a positive note, if this noise is a problem as low as 30KX it
} should be somewhat easier to find.
}
} Regards,
}
} Bob Roberts
} EM Lab Services, Inc.
} Tempe, Arizona 85284
}
}
} Curtis Olson wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
3D Microscopy of Living Cells Course, June 10 - 20, 2002, UBC, Vancouver Canada
Info: ht tp://www.3dcourse.ubc.ca/ Applications due by March 15, 2002


From daemon Tue Feb 5 13:07:33 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 5 Feb 2002 08:56:42 -1000 (HST)
Subject: Rephrased - Nikon Coolpix vs Olympus digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, All-

} From the personal responses I've been getting it seems like a lot of
people haven't figured out that the Olympus C-3030 and C-4040 cameras can,
indeed, be mounted on light microscopes. Olympus can supply all the
adapters needed for this task, and can come in with an easier setup,
better resolution, and a lower price. The main attraction of the Coolpix
seems to be that it can swivel to make viewing the screen easier (I heard
a rumor that this feature is about to be discontinued), but either camera
would benefit by being plugged into a monitor. I haven't had a chance to
compare the cameras, and was hoping to scare up someone who
has. Interestingly, the ones who have replied with information have not
been from the US, so local marketing must be spotty.

} From the replies so far from people who have tried both, one bought the
Nikon, one bought the Olympus.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Tue Feb 5 13:09:22 2002



From: Dave Hall :      daveh-at-restechimage.com
Date: Tue, 5 Feb 2002 14:02:23 -0500
Subject: Straight parallel micron bars in Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All:

I'd like to throw out another possible option for creating micron bars
on images. The PAX-it Basic Measurement Module (as an add-on for the
PAX-it image database product) provides this exact capability. Similar
to other software options suggested previously, the Basic Measurement
Module also offers other measurement capability such as point-to-point
distances, angle measurements, segmented line lengths, parallel line
calipers, etc. There is also an Enhanced Measurement Module adding a
range of additional features for area detection, blob counting, sizing,
and sorting, etc. The measurement modules also provide direct report
generating capabilities through (OLE) Automation links to MS Word,
Excel, or PowerPoint.

However, what PAX-it and its measurement modules offer, that other
options mentioned previously do not appear to offer, is a fully
integrated relational database for images and related documents such as
Word documents, Excel spreadsheets, PDF files, PowerPoint presentations.
Everything relating to a project can be entered into the database as
various records with searchable criteria. The database user-interface
is an easy-to-understand visual presentation using file cabinets,
cabinet drawers, file folders, and thumbnails of the images. And, the
entire database can be placed on a file server and, with a special
module, made available across internal networks or the internet to be
accessed using just a standard web browser interface.

PAX-it is a commercial product and we are a reseller for the product.
Hopefully this message is not inappropriate in this forum because we are
not the only reseller for the product, and we are not directly
soliciting sales of the product through this message. The intent is
simply to raise awareness of another option for image measurement and
management.

The manufacturer for PAX-it is MIS (847-455-0450) and the web page is
www.paxit.com. There are multiple dealers nationwide and interested
parties should contact MIS to find the dealer nearest them. (You might
indicate that you became aware of the product through the Microscopy
ListServer.)

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045
*Columbus, OH 43228


-----Original Message-----
} From: Beauregard [mailto:beaurega-at-westol.com]
Sent: Monday, February 04, 2002 6:38 PM
To: microscopy-at-sparc5.microscopy.com


Hi,
To create an image with a labeled micron bar in 20-30 seconds:
1. Make the info tab totally visible as a separate window on the right
side of PS.
2. Crop your image. I assume you are calibrated in cm.
3. Click on the line drawing tool.
4. In the lower left corner, click where you want the marker to start.
5. Drag to the right slightly, then hold down the shift key for a
perfect
straight line. (Use the option tab to increase the line width (5-15
pixels).)
6. Drag to the right until the D: in the INFO window that shows the
length
you need on the final printed image
You should take into account how you might have changed the DPI values.
For example, if you scanned a neg. at 600 DPI and cut it to 200 DPI,
then a
negative of 10,000X will be about 30,000X on the print and the line
length
for 1µm should be D: 3.00 CM in INFO. This varies a bit with your
printer. Use scanned graph paper to calibrate your DPI to exactly 3x.
I
use 196 DPI on a HP1200TN to get 3X, after scanning at 600 DPI.
7. CAREFULLY release the mouse button, then the shift key and you have
your perfect line.
8. Click on the type tool and click at the position you want the text
above the marker. I use centered, 15 point, crisp, black ink, ariel MT,
bold, etc.
9. Type in using 15 points the length of the line; 1µm, 0.1µm, 0.3
µms, etc.
10.. Highlight the "1µm" text and copy it as a template to the
clipboard
for the next image. Click OK.
11. Continue to the next image.

µ = press and hold down the ALT key,
on the NUMSPAD on the right of the keyboard,
type 0181,
release the ALT key.
µ appears in ANY windows program.
Try:
http://www.dtp-aus.com/ext_set.htm
for more extended characters and a nice table you can print.
Å, ², ³,©, ®, ±, £, ö, *, 0176°

Paul Beauregard
Senior Research Associate
PPG Industries
440 College Park Drive
Monroeville, PA 15146




From daemon Tue Feb 5 14:36:35 2002



From: Ayten Celik :      celik-at-students.uiuc.edu
Date: Tue, 5 Feb 2002 14:29:17 -0600 (CST)
Subject: Re: EM safety and family plan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I have joint this group recently so, I do not know about the previous
discussion. The problem about women radiation workers is that (in my
opinion): At birth each female carries a lifetime supply of egg cells.
These egg cells are not in their final form but anyway they can be damaged
or altered when a female radiation worker is exposed to radiation any
time.

To make it short, it is not only what we are doing during pregnancy, it is
what we have been doing before pregnancy as well.

Recently, I had a baby and I have cancelled my experimental work at
research reactors and my flights during my pregnancy.

Ayten Celik Aktas
-at-UIUC


On Thu, 31 Jan 2002, Leona Cohen-Gould wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} HI Evelyn,
} this question came up a while back,so you may want to scan the
} archives, but here are my 2 cents...
} I have a healthy, happy 9 year old son. I have been doing TEM & SEM
} for 25 years. While I was "family pIanning" and pregnant, I wore
} double gloves and worked in the hood when appropriate, washed my
} hands frequently, followed OSHA guidelines and used common sense. I
} still do (except for the double gloves part). Yes, much of what we
} use can be dangerous to ourselves as well as our progeny, but with
} appropriate care I don't think one needs to take a leave or stop
} doing your job.
}
} JMHO,
} Lee
}




From daemon Tue Feb 5 16:11:01 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Tue, 5 Feb 2002 15:43:01 -0600
Subject: Re: micron bars in Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

My requirements are that I have to put a label as well as a scale bar on
each optical micrograph and burn CD to archive, all with GLP documentation
(we are working towards a 21CFR Part 11 compliant system). I have
calibrated our research microscope with various optical systems and
photoeyepieces as well as a new macroscope and created about 100 scale bars.
We then automated the whole process as much as we could using Actions and
Droplets and a Word macro to create a label as well as a list of all the
files burned on a CD by filling in a form.

Next we are hoping to be able to write a plug-in that will create a history
text file to document each change to a photograph. I asked Adobe if they
could do that and the person said that it would be feasible but apparently
not enough demand, yet.

Damian Neuberger, PhD
Senior Research Scientist
Baxter Healthcare Corp.
Tel: 847.270.5888
damian_neuberger-at-baxter.com


From daemon Tue Feb 5 16:52:34 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 5 Feb 2002 18:23:07 -0500
Subject: EELS Raw Data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I evaluated the Nikon Coolpix 990 and Olympus C-3040 for use on my Olympus
SZX12 stereomicroscope when working on-site. When asked, I recommend both
cameras.

I chose the C-3040 because Olympus sells a fixed tubelength adapter
(C2000Z-ADP) that mounts to the trinocular head of the Olympus
stereomicroscope (and my Continuum infrared microscope, which was
manufactured using Olympus components). The adapter makes the camera
parfocal on Olympus microscopes -- no need to focus an adapter. Also, the
C-3040 comes with a remote shutter release as a standard accessory.

Performance on and off the microscopes has been outstanding. In addition to
making still micrographs, I use the camera to record digital video of
solubility and microchemical tests for clients and colleagues. The live
video out function makes focusing and instruction easy (I use a Sony
Trinitron 13 monitor on road trips, or an inexpensive monochrome monitor
when I fly).

I also used the camera to record my son's first rookie hit in Little League.
What does the commercial say? Priceless.

James Martin
Orion Analytical, LLC
Post Office Box 550
Williamstown, MA 01267
t: 413-458-0233 f. 413-458-5542
www.orionanalytical.com


----- Original Message -----
} From: "Tina Carvalho" {tina-at-pbrc.hawaii.edu}
To: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, February 05, 2002 1:56 PM


Are you referring to the EMSA format when you are referring to the ZAL format? If you are, there is a program in the EMSA/MAS library that I put in called "EELS_Plot" this last summer. I am about to send Nestor a newer version of this to replace it. It will plot EELS and EDS data in EMSA format and do a few other things as well. If you want to color, display, compare, subtract background, rescale, and display up to five spectra, copy to window applications, and keep notes, this program will do it. If you can't wait, send me your address, and I will send you a copy on CD.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Pradyumna Prabhumirashi [mailto:p-prabhumirashi-at-northwestern.edu]
Sent: Monday, February 04, 2002 11:25 PM
To: Microscopy Listserver (Microscopy Listserver)



Hello,

I need some help in converting some raw EELS data into formats readable
by either EL/P or digital micrograph. The data I have gathered is in
.ZAL (Zaluzek) format. The data looks very similar to the .TAD format.
Let me know if there is a program that reads .ZAL format and converts it
to .TAD or even two column text. Perhaps Nestor would be able to help in
this regard, right? :).
Thanks in advance,

Prad

Pradyumna Prabhumirashi
Dept. of Materials Science
Northwestern University
Phone: (847)491-7798
Fax : (847)491-7820




From daemon Tue Feb 5 17:34:33 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 5 Feb 2002 17:29:16 -0600
Subject: EDS Noran file availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are using a Noran Voyager 3.3 energy dispersive analyzer to
generate linescan analyses across catalyst granules. We would like to
obtain the data in a spreadsheet format (like Excel or even ASCII)
for use outside of the Noran system. We are able to open regular
analyses saved in the MSA format but the linescans can not be saved
in this format.

Does anyone know how this could be done?

As always, we are very appreciative of any help that you might provide.

JB

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Tue Feb 5 18:42:16 2002



From: DrJohnRuss-at-aol.com
Date: Tue, 5 Feb 2002 19:34:25 EST
Subject: Re: micron bars in Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 2/5/02 5:11:37 PM, neuberger1234-at-attbi.com writes:

} Next we are hoping to be able to write a plug-in that will create a history
} text file to document each change to a photograph. I asked Adobe if they
} could do that and the person said that it would be feasible but apparently
} not enough demand, yet.

The need to document each step in the image processing chain is also
important for forensic applications. You should check with Chris Russ at
Reindeer Graphics (jcr6-at-AOL.com), who has been working on that need and may
have some ideas or even a plug-in for you to use.



From daemon Tue Feb 5 19:11:06 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 05 Feb 2002 20:07:21 -0800
Subject: Re: EM safety and family plan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Leona,

What worked for this lab was to ask the female employee in
question to inform you when she intended to become pregnant and then to
arrange for someone in the lab to take responsibility for sample
preparation during the "intention period", 9 months of pregnancy, the
maternity leave and the additional months during which the mother was
nursing the child. This amounted to a substantial amount of time in our
case but I felt better about taking on the added responsibility rather than
deal with possibly serious consequences after the fact.
Rosemary Walsh




From daemon Tue Feb 5 20:21:45 2002



From: gtg990a-at-prism.gatech.edu
Date: Tue, 05 Feb 2002 22:14:00 -0500 (EST)
Subject: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Russ,
We have already contacted Chris and are working on an agreement to help us,
or write for us, the plug-in(s). though someone who reports to me is
handling this, I will be calling Chris myself to discuss it. It is
something that I would like to make available to everybody when done,
perhaps through the IPTK next version.

Damian Neuberger
P.S. I hope that I can take the class this time around, last time I had to
cancel due to travel restrictions.


----- Original Message -----
} From: {DrJohnRuss-at-aol.com}
To: {neuberger1234-at-attbi.com} ; {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, February 05, 2002 6:34 PM


To whom it may concern:

I am an undergraduate student enrolled at the Georgia Institute of Technology.
I am in a biomedical engineering class, and we have a problem that we must
solve involving electron microscopes. I have a few questions that I hope will
get answered ASAP:

1. What are the advantages and disadvantages in using electrons for microscopy
rather than light?
2. Does the wavelength of the electrons have anythign to do with the spatial
resolution that the microscope produces in the final picture?
3. What is temporal resolution and how is it produced in the electron
microscope?

Thank you for your time. I greatly appreciate your efforts in helping me
understand more of this subject.

Sincerely,
Jenny Wang

-------------------------------------------------
Sent through Cyberbuzz- A Server for the Students
http://cyberbuzz.gatech.edu/


From daemon Wed Feb 6 02:26:50 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Wed, 06 Feb 2002 09:17:31 +0100
Subject: LM - geometry and analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am looking around for information about geometry based algorithms for
analysis of microscopy images. I have some experience with this kind of
algorithms, which are extremely powerful but somewhat CPU-hungry and I
am looking for improvements.

On the top my personal website you will find examples about what I mean
with geometry based analysis of microscopy images of cells and tissues:

http://ourworld.compuserve.com/homepages/pvosta/cvwww.htm

By the way I also made a webpage about microsocpy and imaging, which
could be useful
for other people too:

http://ourworld.compuserve.com/homepages/pvosta/pcrimg.htm

Best regards,

Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

WWW: www.unionbio.com


From daemon Wed Feb 6 03:26:34 2002



From: David Vowles :      djv23-at-msm.cam.ac.uk
Date: Wed, 6 Feb 2002 09:19:58 +0000 (GMT)
Subject: Re: EDS Noran file availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

You wrote:

} We are using a Noran Voyager 3.3 energy dispersive analyzer to
} generate linescan analyses across catalyst granules. We would like to
} obtain the data in a spreadsheet format (like Excel or even ASCII)
} for use outside of the Noran system. We are able to open regular
} analyses saved in the MSA format but the linescans can not be saved
} in this format.
}
} Does anyone know how this could be done?

I have written an application to read the various Voyager file formats
(.eds,.lscan,.grey,.xray) on a PC and display/export data in text format.
For the linescan format it will display the image file with line and each
of the linescans graphs. These can be exported to a text file for use in
Excel, etc. I can let you have a copy of this if you wish.


David Vowles
Electron Microscope Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 1223 334325
Fax: +44 1223 334567
Email: djv23-at-cam.ac.uk




From daemon Wed Feb 6 04:23:56 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Wed, 06 Feb 2002 11:17:21 +0100
Subject: LM - geometry and analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am looking around for information about geometry based algorithms for
analysis of microscopy images. I have some experience with this kind of
algorithms, which are extremely powerful but somewhat CPU-hungry and I
am looking for improvements.

On the top my personal website you will find examples about what I mean
with geometry based analysis of microscopy images of cells and tissues:

http://ourworld.compuserve.com/homepages/pvosta/cvwww.htm

By the way I also made a webpage about microsocpy and imaging, which
could be useful
for other people too:

http://ourworld.compuserve.com/homepages/pvosta/pcrimg.htm

Best regards,

Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

WWW: www.unionbio.com


From daemon Wed Feb 6 08:43:28 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 6 Feb 2002 08:34:47 -0600
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think your instructor's hope would be that you figured out the
answers to class problems on your own. Asking an expert in the field
and then simply regurgitating that information is a worthless
exercise. If you are going to invest the time and money required to
earn a degree, you might want to try to learn something along the
way. I am a big supporter of listservers but hate to see them used
in this way.


}
}
} To whom it may concern:
}
} I am an undergraduate student enrolled at the Georgia Institute of
} Technology.
} I am in a biomedical engineering class, and we have a problem that we must
} solve involving electron microscopes. I have a few questions that I hope will
} get answered ASAP:
}
} 1. What are the advantages and disadvantages in using electrons for
} microscopy
} rather than light?
} 2. Does the wavelength of the electrons have anythign to do with the spatial
} resolution that the microscope produces in the final picture?
} 3. What is temporal resolution and how is it produced in the electron
} microscope?
}
} Thank you for your time. I greatly appreciate your efforts in helping me
} understand more of this subject.
}
} Sincerely,
} Jenny Wang
}
} -------------------------------------------------
} Sent through Cyberbuzz- A Server for the Students
} http://cyberbuzz.gatech.edu/

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Feb 6 09:19:29 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 6 Feb 2002 08:15:31 -0700
Subject: Re: micron bars in Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Damian,

as you have probably noticed, 21CFR Part 11 is a pretty tough cookie, and in
fact involves more than one piece of software. If you read it closely,
you'll find that it involves the operating system, as well as Operating
Procedures, which are outside the realm of any single software. We have
found a solution to this, but I don't want to advertise this here, so please
contact me off-line if you are interested.

Also, regarding the micron bars, I'd like to mention, that this is always an
afterthought for Photoshop. John Russ has done a good job providing some
features to put a scale bar on the image, but for a comprehensive solution,
I think there are other solutions. We, for example (and other programs
probably as well) make sure, that an image is calibrated from the start by
either reding the calibration or magnification from the instrument or
entering the magnification by hand. The scale bar is then displayed at any
time in the viewport. We found, that a scale bar attached to the image or
the overlay is not the best solution in many cases. For example, if you have
a large image, the size of the scale bar depends on the "screen
magnification": if you set that to 100%, the scale bar has a good size to
see, but you may not see it because the image does not fit on the screen. If
you fit the image to the screen, the scale bar may be too small to read. We
tried to solve this problem by calculating the scale bar dynamically and
displaying it as a part of the viewport, not the image (unless, of course,
you want to attach it to the image).

Finally, I'd like to announce that we moved to new locations in Lakewood.
Please make a note of our new address.

Mike Bode

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Damian Neuberger [mailto:neuberger1234-at-attbi.com]
Sent: Tuesday, February 05, 2002 2:43 PM
To: microscopy-at-sparc5.microscopy.com


Listers,

My requirements are that I have to put a label as well as a scale bar on
each optical micrograph and burn CD to archive, all with GLP documentation
(we are working towards a 21CFR Part 11 compliant system). I have
calibrated our research microscope with various optical systems and
photoeyepieces as well as a new macroscope and created about 100 scale bars.
We then automated the whole process as much as we could using Actions and
Droplets and a Word macro to create a label as well as a list of all the
files burned on a CD by filling in a form.

Next we are hoping to be able to write a plug-in that will create a history
text file to document each change to a photograph. I asked Adobe if they
could do that and the person said that it would be feasible but apparently
not enough demand, yet.

Damian Neuberger, PhD
Senior Research Scientist
Baxter Healthcare Corp.
Tel: 847.270.5888
damian_neuberger-at-baxter.com


From daemon Wed Feb 6 09:50:41 2002



From: RCHIOVETTI-at-aol.com
Date: Wed, 6 Feb 2002 10:42:49 EST
Subject: Re: NIST-traceable stage micrometer?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 02/05/2002 8:42:07 AM US Mountain Standard Time,
David_R_Stadden-at-armstrong.com-at-sparc5.microscopy.com writes:

{ { I've been asked if the stage micrometers I use for calibrating our optical
scopes are NIST-traceable. Is there such a thing and, if not, what would
satisfy requirements for ISO certification in the area of metrology?

Thanks!

Dave Stadden
Research Scientist
Armstrong World Industries, Inc.
Lancaster, PA
} }

Dave,

Yes, there is such a thing. You can get a stage micrometer that's
NIST-traceable from:

Klarmann Rulings
480 Charles Bancroft Highway
Litchfield, NH 03052
Tel. 800-252-2401
Fax 603-424-0970

If I recall correctly, you pay a minimum fee for certification of a set
number of points on the scale (you can specify which points on the scale are
to be certified). You can also pay a minimal fee to certify additional
points above the minimum number.

The folks at Klarmann can help you with this.

Good luck!

Bob Chiovetti
GTI Microsystems


From daemon Wed Feb 6 10:54:45 2002



From: Brian Wajdyk :      electronmicroscopist-at-hotmail.com
Date: Wed, 06 Feb 2002 09:46:49 -0700
Subject: measurement and calibration onthe SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists,

SEM has historically been used for metrology of various structures. I can't
seem to find much literature about the artifacts associated with this type
of measurement. We do use the NIST standards to check the calibration of
our equipment, but I haven't characterized how the different beam or sample
parameters effect the measurements. What do you do? Has anyone figured out
his or her actual accuracy and precision? We have found that we can safely
give measurements within +/-5% taking into account most human and equipment
errors. This is based on the precision of measurements made of NIST
structures, measured the same way, over several years. On the other hand,
the smallest structure we can measure on the standard is 2um (line and
space. How do you determine at what magnification you will no longer
guarantee the measurement? I see that my MRS-3 from Geller says it's for
10x to 50kX. How do they figure out that the max magnification it is
useful? Maybe it as simple as being able to fit the structure on the
screen. If that were true, you would expect that the instrument would also
be calibrated to a much higher magnification. How high could I say it is
accurate to? Can I safely measure a 1000A line assuming no obvious issues
(i.e. drift)? Can anyone educate me more on this topic or point me to
resources?


Things that could effect measurements (feel free to add to list):
Drift (mechanical / beam)
Charging (obvious or stretching of image from a slow scan)
Magnification (adjusted for each set of lens relays)
kV (surface vs. subsurface image)
Working Distance
Delineation method (raised vs. depression, materials contrast)
Amount of delineation (3D effect)
Resolution (near resolution limit of SEM?)
Contrast (or lack of, bright / dark line)
Edge effect (bright line)
Consistency between tools (calibration, etc.)
Consistency between methods (SEM, TEM, ellipsometry, AFM, etc.)
Operator's eye (where to measure. Measure outside to inside, center to
center, out to out, in to in?)
Variance in measured layer thickness (topography, sloped profile (i.e. base
larger than top))
Angle to beam
Preparation methods (polish (i.e. smearing), cleave (i.e. pull of soft
material), FIB (i.e. angle))
Type of algorithm if doing it automatically (i.e. %50 threshold)





_________________________________________________________________
MSN Photos is the easiest way to share and print your photos:
http://photos.msn.com/support/worldwide.aspx



From daemon Wed Feb 6 11:02:13 2002



From: Dave Hall :      daveh-at-restechimage.com
Date: Wed, 6 Feb 2002 11:54:52 -0500
Subject: NIST-traceable stage micrometer?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, NIST-traceable stage micrometers do exist. At least one place they
are available from is Klarmann Rulings (www.reticles.com).

We have utilized these NIST-traceable micrometers in previous systems
we've implemented with much success.

Good Luck

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045
*Please visit our website at http://www.restechimage.com to find our
Optimas training schedule and other useful information.


-----Original Message-----
} From: David_R_Stadden-at-armstrong.com
[mailto:David_R_Stadden-at-armstrong.com]On Behalf Of
"David_R_Stadden-at-armstrong.com"-at-sparc5.microscopy.com
Sent: Tuesday, February 05, 2002 10:29 AM
To: Microscopy-at-sparc5.microscopy.com




I've been asked if the stage micrometers I use for calibrating our
optical
scopes are NIST-traceable. Is there such a thing and, if not, what
would
satisfy requirements for ISO certification in the area of metrology?

Thanks!

Dave Stadden
Research Scientist
Armstrong World Industries, Inc.
Lancaster, PA





From daemon Wed Feb 6 12:04:01 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 6 Feb 2002 11:56:15 -0600
Subject: UPS systems and microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a space in my lab for installation of
2 UPS systems (6 and 8 kVa) from Hitachi. One of the options
is to install them in the same room where ultramicrotome is.
I do not like this option, but space is tight. I appreciate
any comments about microtome performance in the presence of
UPS. Microtome is sitting on the air antivibration table.

Thank you,

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



From daemon Wed Feb 6 12:05:07 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 06 Feb 2002 13:00:41 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Tom Phillips wrote:

} I think your instructor's hope would be that you figured out the
} answers to class problems on your own. Asking an expert in the field
} and then simply regurgitating that information is a worthless
} exercise. If you are going to invest the time and money required to
} earn a degree, you might want to try to learn something along the
} way. I am a big supporter of listservers but hate to see them used
} in this way.

Good for you!!

I sent the young lady the same message via private e-mail. I wonder if the
instructor knows about this sort of "research"?

} } To whom it may concern:
} }
} } I am an undergraduate student enrolled at the Georgia Institute of
} } Technology.
} } I am in a biomedical engineering class, and we have a problem that we must
} } solve involving electron microscopes. I have a few questions that I hope will
} } get answered ASAP:
} }
} } 1. What are the advantages and disadvantages in using electrons for
} } microscopy
} } rather than light?
} } 2. Does the wavelength of the electrons have anythign to do with the spatial
} } resolution that the microscope produces in the final picture?
} } 3. What is temporal resolution and how is it produced in the electron
} } microscope?
} }
} } Thank you for your time. I greatly appreciate your efforts in helping me
} } understand more of this subject.
} }
} } Sincerely,
} } Jenny Wang
} }
} } -------------------------------------------------
} } Sent through Cyberbuzz- A Server for the Students
} } http://cyberbuzz.gatech.edu/
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Feb 6 12:33:21 2002



From: curari-at-asu.edu
Date: Wed, 06 Feb 2002 11:27:12 -0700 (MST)
Subject: ISI SX-40 Help w/ type of video signal?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Lister

I would like to thank those who gave me some input to my first
delimma.

I found that there are a ton of different frame grabbers out
there. What I dont know is what kind of output signal the scope produces, as
far as my understanding of the ISI SX-40 SEM it puts a signal out to the
CRT. Some of the venders' questions have been if it is an NTSC, PAL, or
RS170 type signal.I am slightly familiar with the first two, but the latter I
have no idea. I do know that there are a couple of "off the shelf" products
from GW Electronics and Image Slave, but these setups are priced at
$4000+. So what I am looking for is if someone
can point me in the direction of where I can reseach this information.



Wil

This email was sent with 100.00% recycled electrons.

P.S. This is a project that hopefully will get students involved with
microcopy from our Biology and Chemistry departments in order to familiarize
the students with instrumentation available in their area of study.



From daemon Wed Feb 6 13:38:25 2002



From: Jinguo Wang :      jqw11-at-psu.edu
Date: Wed, 06 Feb 2002 15:27:20 -0500
Subject: Epson scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Torino 06 February 2002

Dear Dr. P. Van Osta,

you could try this website of Florence University:

http://www.unifi.it/unifi/dbag/lbs1/lbsdip.htm

(sorry...it is in Italian)
and mail to Dr. Stefano Bianchi at this address:
stefano.bianchi-at-dbag.unifi.it

Good luck.
Best Regards,
Ing. Massimo Tosi
----- Original Message -----
} From: "Peter Van Osta" {pvosta-at-unionbio-eu.com}
To: "Microscopy" {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, February 06, 2002 9:17 AM


Dear Lister:

I have a Epson scanner (Perfection 1200 Photo) with a transparency adaptor.
Recently, we have some problems when we scan the negative films. The
pre-scan image looks pretty nice after some adjustment, but the final scan
image is too dark almost just a piece of black stuff.
Any help will be appreciated.

Thanks

Jinguo Wang










From daemon Wed Feb 6 14:35:22 2002



From: tartenon-at-netscape.net
Date: Wed, 06 Feb 2002 15:28:35 -0500
Subject: RE: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
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Nikon Coolpix 995 has 2.3Megapixels non-interpolated

Tina Carvalho {tina-at-pbrc.hawaii.edu} wrote:

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From daemon Wed Feb 6 15:03:04 2002



From: NPGSlithography-at-aol.com
Date: Wed, 6 Feb 2002 15:54:32 EST
Subject: Re: Environmental Noise Impacting JEOL 848 SEM

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Dear Curtis,

} I operate a JEOL 848 SEM but am limited to less than 30,000x due to at
least
} two frequencies of external noise. The service engineers from JEOL have
} determined the noise is not due to the SEM. I will be conducting as much of
a
} room survey as possible later in February but I am hoping to obtain some
} insight into common testing formats, noise sources, and solutions. From
what
} I have been able to learn so far, this noise reduction is something of an
art
} rather than science. Thank you in advance from this microscopist with much
to
} learn.

I agree that the other responses have very good points.

In addition, if the problem is actually caused by magnetic fields interacting
with the beam, it should get worse at lower kV and at longer working
distances. Do you know the frequencies of the interference? If so, that can
be a very good clue as to its source.

Also, a digital gauss meter that I have found very useful for locating
sources of magnetic fields can be purchased for under $100 US [see the Extech
Model #480823 at www.MetersandInstruments.com, (800) 773-0370, - I have no
financial interest in this company]. This meter makes frequency independent
RMS readings between 30 and 300 Hz. Since line frequency and harmonics are
the most common fields, it works very well.

With such a digital meter, or even a coil of wire connected to an
oscilloscope to act as an uncalibrated magnetic field meter, you can first
check for field strength near the chamber. If a significant magnetic field
(i.e., } 1 mG rms) is observed, it can often be tracked back to the source by
moving the meter around. If the field simply "fills the room", it may be
from a power line with an unbalanced load. For example, if the current runs
on the "hot" wire, but does not return on the neutral or ground wire, then a
large magnetic field will be generated, while normally there are equal and
opposite currents which cancel at any significant distance. In this case,
the wires themselves can be outside the room and the actual wiring problem
may be anywhere in the building!

As a final comment, a good check on environmental problems is simply to come
in after hours when other equipment is more likely to be turned off (or to go
around and turn off everything that you can, but that is typically limited to
your immediate lab). For example, I have watched a gauss meter go from
showing a low field to a significant field simultaneously as an image at high
mag degraded with line frequency interference. The source of the field was
discovered to be power lines outside the SEM room that supplied power to a
laser several labs away. When the laser operator started working in the
morning and increased the laser power, the meter and SEM display clearly
showed the interference.

Good luck.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Wed Feb 6 15:11:24 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 6 Feb 2002 11:05:00 -1000 (HST)
Subject: RE: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
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****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Wed Feb 6 15:39:49 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 6 Feb 2002 15:30:35 -0600
Subject: RE: measurement and calibration onthe SEM

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} -----Original Message-----
} From: Brian Wajdyk [mailto:electronmicroscopist-at-hotmail.com]
} Sent: Wednesday, February 06, 2002 10:47 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: measurement and calibration onthe SEM
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Fellow Microscopists,
}
} SEM has historically been used for metrology of various
} structures. I can't
} seem to find much literature about the artifacts associated
} with this type
} of measurement. We do use the NIST standards to check the
} calibration of
} our equipment, but I haven't characterized how the different
} beam or sample
} parameters effect the measurements. What do you do? Has
} anyone figured out
} his or her actual accuracy and precision? We have found that
} we can safely
} give measurements within +/-5% taking into account most human
} and equipment
} errors. This is based on the precision of measurements made of NIST
} structures, measured the same way, over several years. On
} the other hand,
} the smallest structure we can measure on the standard is 2um
} (line and
} space. How do you determine at what magnification you will no longer

Cheap grating replicas with 2600 lines per mm give 0.46 um per line,
and this is not too bad for calibrating 50,000 magnification. I am
happy I do not need certified standards.

} guarantee the measurement? I see that my MRS-3 from Geller
} says it's for
} 10x to 50kX. How do they figure out that the max magnification it is
} useful? Maybe it as simple as being able to fit the structure on the

Maximum useful magnification is very specimen dependant, especially
for low voltage and low vacuum modes. Of course, for digital images
it is possible to check brightness profiles and if they have slopes
on edges of features, then measure "size" on half height of the slope.
But I am not aware about publications which dependably justify this kind
of measurements (manipulations with brightness and contrast and
specimen tilt could change slopes significantly).

} screen. If that were true, you would expect that the
} instrument would also
} be calibrated to a much higher magnification. How high could
} I say it is
} accurate to? Can I safely measure a 1000A line assuming no

It depends on resolution for your microscope/specimens and on
calibration standard you are using. And I think periodic lines with
spacing 2 um not really good standard to measure feature with
the size of 0.1 um.

} obvious issues
} (i.e. drift)? Can anyone educate me more on this topic or

If you have visible drift during single exposure, then something
wrong with microscope or specimen preparation technique.

} point me to
} resources?
}
}
} Things that could effect measurements (feel free to add to list):
} Drift (mechanical / beam)-

exposure time should be small for significant drift.

} Charging (obvious or stretching of image from a slow scan)
} Magnification (adjusted for each set of lens relays)

Could be eliminated with proper calibration.

} kV (surface vs. subsurface image)
} Working Distance

Could be eliminated with proper calibration.

} Delineation method (raised vs. depression, materials contrast)
} Amount of delineation (3D effect)
} Resolution (near resolution limit of SEM?)
} Contrast (or lack of, bright / dark line)
} Edge effect (bright line)
} Consistency between tools (calibration, etc.)
} Consistency between methods (SEM, TEM, ellipsometry, AFM, etc.)
} Operator's eye (where to measure. Measure outside to inside,
} center to
} center, out to out, in to in?)
} Variance in measured layer thickness (topography, sloped
} profile (i.e. base
} larger than top))
} Angle to beam
} Preparation methods (polish (i.e. smearing), cleave (i.e.
} pull of soft
} material), FIB (i.e. angle))
} Type of algorithm if doing it automatically (i.e. %50 threshold)

Some of the things you have mentioned relate to specimen/experiment,
to stereology, but not to microscope. For example, if I need to measure
size of depression without sharp edges, I have to find (or at least to
declare)right procedure for it's measurements. May be I have to perform
stereo measurements and define an edge as a place, where a depth of
depression become equal to 0.1 um (or 10% of total depth, or
whatever else, depending on a study).

And thank you for your extensive list - it is very helpful for
observation of the problem. And about additions to your list -
I think everybody can say something. For example recently I tried
to measure in ESEM thickness of a layer which, as it turned out,
was a viscous liquid...

Regards,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



From daemon Wed Feb 6 15:41:31 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 6 Feb 2002 11:35:53 -1000 (HST)
Subject: RE: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Wed, 6 Feb 2002 tartenon-at-netscape.net-at-sparc5.microscopy.com wrote:

} Nikon Coolpix 995 has 2.3Megapixels non-interpolated

You made me check into this! Nikon advertises 3.2 megapixels for the
Coolpix 995, and Olympus advertises 4 megapixels for the C-4040, but BOTH
really deliver 2048 x 1536 pixels. Interesting marketing.

They are basically the same technology, with the only difference being the
menus and availability of adapters, I guess. And now all the adapters are
available all kinds of places, so I guess it's a matter of taste. There
aer other manini (a manini is a small fish) differences in number of
threads, recording media, stuff like that.

Mahalo,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Wed Feb 6 16:18:50 2002



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Wed, 6 Feb 2002 14:55:44 -0700
Subject: Ceramic Insulator for Hitachi H800 TEM

Contents Retrieved from Microscopy Listserver Archives
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Hello!

We have a Hitachi H800 TEM (200 Kev,LaB6) and a few days ago we started having problems with the HV. Static discharges
in the gun area (even at 75 KeV) were traced back to the failed insulator. Hitachi is trying to get us new one, but they
tell me it could take several months since this is normally not a stock item. If any body out there has one that they
are willing to part with (for money) or if any body has other suggestions I would really appreciate your input.

Thanks

Jordi Marti


From daemon Wed Feb 6 17:59:53 2002



From: Mark YEADON :      m-yeadon-at-imre.org.sg
Date: Thu, 7 Feb 2002 10:49:15 +0800
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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RS-170 refers to composite b/w video. NTSC refers
to composite color video added to the RS-170 format.
Composite means that the video signal and sync
pulses (H & V & blanking) are conveyed via a single wire.

If I recall the SX-40 correctly (let me know if I am
wrong on this), it has a TV rate display for focusing
and stig and eye-ball viewing, but image recording is/was
via the slow scan high rez short persistence 2K line
film recorder CRT. This poses a significant problem
for image capture.

RS-170 video is interlaced between even/odd lines (fields).
One frame (two fields) is not presented
at the same time. The rapid scan (60/s per field)
and the persistence of the viewing CRT, fool the eye
into being able to see integrated sets of lines. A
frame grabber can't be fooled. The purpose of interlacing
is to reduce flicker which would occur if the whole frame
were sent at one time.

The RS-170 format is essentially a frame of 640x480
pixels. But the problem is that to capture the whole
frame, one needs to store the even and odd fields
and then grab the frame. This feature is typically
called an image buffer or frame buffer. Its output
is RS-170 but consisting of a full frame, either
realtime or the result of slow scan.

I don't think you have this in the SX-40. Your only
option, as I see it, is to get a passive capture
system which is attached to the recording CRT.
The passive capture systems connect to the
recording CRT's H & V sync pulses and the
blanking pulse. GW makes passive capture systems,
Soft Imaging does, as do others. But as you have
found, these are not cheap systems. In some
cases, the hardware is not particularly complex.
But the software is.

The passive system follows the scan pulses
from the SEM's scan generator and uses an
A/D converter to sequentially digitize the output
of the SE detector. A challenge with this is
to obtain fidelity between what is seen versus
what is captured. i.e., same contrast and
brightness on the viewing screen as on the
captured image. Doable, once set up properly.

Perhaps you could elicit the assistance of the
Electrical Engineering Department? Some
clever grad student might love to undertake a
project to digitize your SEM.

gary g.


At 10:27 AM 2/6/2002, you wrote:
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Wil,

the answer is fairly simple:

A "video signal" is commonly referred to if the signal follows the standards
set for commercial TV. This allows you to take a camera and connect it
directly to a TV or VCR. As you probably know, there are different standards
in the US and Europe. NTSC is the signal used for color data in the US and
Japan, PAL is the signal used for color data in Europe and other places,
RS170 is for b/w signals. Some frame grabbers can understand all of these
signals, others only one or two.

The problem with a video signal is, that the resolution is very low
(480x640x8bits for NTSC).

If you use the SEM in "slow scan mode", most frame grabbers will not be able
to detect the correct signal because it does not conform to the NTSC or PAL
standards anymore. Then you need to get special electronics, which is not
made in large numbers and is therefore more expensive.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "curari-at-asu.edu"-at-sparc5.microscopy.com
[mailto:"curari-at-asu.edu"-at-sparc5.microscopy.com]
Sent: Wednesday, February 06, 2002 11:27 AM
To: MSA


Dear Lister

I would like to thank those who gave me some input to my first
delimma.

I found that there are a ton of different frame grabbers out
there. What I dont know is what kind of output signal the scope produces,
as
far as my understanding of the ISI SX-40 SEM it puts a signal out to the
CRT. Some of the venders' questions have been if it is an NTSC, PAL, or
RS170 type signal.I am slightly familiar with the first two, but the latter
I
have no idea. I do know that there are a couple of "off the shelf" products
from GW Electronics and Image Slave, but these setups are priced at
$4000+. So what I am looking for is if someone
can point me in the direction of where I can reseach this information.



Wil

This email was sent with 100.00% recycled electrons.

P.S. This is a project that hopefully will get students involved with
microcopy from our Biology and Chemistry departments in order to familiarize
the students with instrumentation available in their area of study.



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Message-ID: {FEBD650DBBBFD311AF1D009027CC7DD701366A1A-at-exchange.imre.org.sg}


Dear Jenny,

We're glad that you are interested in microscopy, and that you did some
research to find this list: below are a few pointers to get you started.
There are some excellent texts on the subject, some of which I cite below
from my own lecture course, but check out your own library too. Also, there
are some very well known electron microscopists at Georgia Tech whom you
could consult with - be brave and go say hi! You will find the majority of
scientists (microscopists included) are always glad of an excuse to wax
eloquent on their favorite subject, esp over coffee. I guess the best
advice is always to talk to the experts, unless forbidden by your instructor
(which I would doubt - our instructors always encouraged our intiative in
these matters!)

Best wishes for your studies,

Mark

%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119260
http://www.matsci.nus.edu.sg/STAFF/Mark.html

TEL: (+65) 874 8591
FAX: (+65) 872 0785
Email: m-yeadon-at-imre.org.sg


} -----Original Message-----
} From: "gtg990a-at-prism.gatech.edu"-at-sparc5.microscopy.com
} [SMTP:"gtg990a-at-prism.gatech.edu"-at-sparc5.microscopy.com]
} Sent: Wednesday, February 06, 2002 11:14 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Questions on the Electron Microscope
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To whom it may concern:
}
} I am an undergraduate student enrolled at the Georgia Institute of
} Technology.
} I am in a biomedical engineering class, and we have a problem that we must
}
} solve involving electron microscopes. I have a few questions that I hope
} will
} get answered ASAP:
}
} 1. What are the advantages and disadvantages in using electrons for
} microscopy
} rather than light?
[Mark YEADON]
couple of advantages: electrons have a shorter wavelength (much
shorter); since resolution in this case is dependent on wavelength (Abbe's
expression - resolution ~ 0.61 x wavelength / sin alpha [see texts for
fuller explanation]) - in principle we can resolve distances of a fraction
of an atom with 100kV electrons). Also, electrons are IONIZING radiation,
and we can detect signals arising from ionization processes when they
interact with our sample (such as characteristic x-rays, charcteristic of
the atoms the electron has interacted with in your sample)

couple of disadvantages: electrons require a vacuum if you want
them to travel enough distance to be of use in an electron microscope. this
is expensive and requires substantial maintenance. electron guns and
columns are much bigger and more complex than a regular light microscope -
equates to dollars!

However, because the value of alpha (in above equation) is so much
smaller for electron microscopes you also get an amazingly high depth of
field (see texts below for a derivation - esp the first three). ie, you can
see 3D objects such as bugs in clear focus over the entire sample in a
scanning electron microscope with marvelous resolution, whereas in the light
microscope only one small part of the bug will be in focus at high
magnification...

} 2. Does the wavelength of the electrons have anythin to do with the
} spatial
} resolution that the microscope produces in the final picture?
[Mark YEADON] Absolutely - see above.

} 3. What is temporal resolution and how is it produced in the electron
} microscope?
}
[Mark YEADON] Spatial resolution relates to dimensions of distance,
for a 200kV TEM with thermionic emission gun you would expect about
2Angstroms spatial resolution. Temporal resolution would relate to time,
and I'm guessing you're thinking of the time taken to capture images - this
would depend upon the imaging system you are using. How quickly can you
record 'frames', one after the other. We get 1/30s temporal resolution for
in-situ experiments quite ok. In principle you can go much better than this
with a very fast CCD camera and a high intensity electron beam. (The more
electrons you have per unit time, the more electrons you can get per frame,
and the better the signal to noise ratio - the limit is usually the
recording equipment (signal to nosie ratio of the CCD chip) and not the TEM.

} Thank you for your time. I greatly appreciate your efforts in helping me
} understand more of this subject.
[Mark YEADON]
You're most welcome. We love our subject and are delighted to help
you in your understanding. See if you have any of the following in your
library, although there are many other good ones also:

Transmission Electron Microscopy, DB Williams and CB Carter
Electron Microscopy and Analysis, Goodhew and Humphreys
Light and Electron Microscopy, Slayter and Slayter
Handbook Of Microscopy, ed. by S. Amelinckx et al., Wiley -VCH

} Sincerely,
} Jenny Wang
}
} -------------------------------------------------
} Sent through Cyberbuzz- A Server for the Students
} http://cyberbuzz.gatech.edu/


From daemon Thu Feb 7 00:58:03 2002



From: pjfenneran-at-msn.com ()
Date: Thu, 7 Feb 2002 00:39:32 -0600
Subject: Ask-A-Microscopist:TEM of Ecoli bacteria

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (pjfenneran-at-msn.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
February 6, 2002 at 10:05:13
---------------------------------------------------------------------------

Email: pjfenneran-at-msn.com
Name: Patrick Fenneran

Organization: Florida Institute of Technology

Education: Graduate College

Location: Melbourne, Florida

Question: I am using an Zeiss 900m TEM looking at E.coli and have the
following questions:

1. The formvar/carbon grids keep on getting blown out, like there is
too much power or the beam is too concentrated.

2. When I am taking pictures, the bacteria do not have resolution,
which adjustments should I make?

3. Do you know of any paperwork that can be obtained that explains
the operation the components of the machine, the only book I have is
the one that came with the machine and it is partly in German.



---------------------------------------------------------------------------


From daemon Thu Feb 7 02:45:17 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 7 Feb 2002 08:48:55 +0000 (GMT Standard Time)
Subject: Re: Ceramic Insulator for Hitachi H800 TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi Jordi,

Sorry to hear about the insulator.

It depends exactly what you mean by 'failed' but we have
had success cleaning ceramic insulators that have been
subjected to tracking of the HT. Even quite deep arcing
tracks have been cleaned out and when returned to service
have worked OK. If they are on the vacuum side they are
just left alone, if they are on a greased joining surface
we ensure that the grease gets well down into the cleaned
track.

I don't know the type of insulator Hitachi use on your
machine but you may be able clean tracks out by polishing
with Al2O3 paste (5um Al2O3 in alcohol). If this does not
work try shot blasting the ceramic with clean Al2O3 beads.
After polishing or blasting blow off with clean N2 (from a
regulated cylinder), clean in several washes of alcohol in
an ultrasonic bath until there is no trace of alumina in
the alcohol and bake out in a clean vacuum oven overnight.
If you cannot get a vacuum oven then heating in air and a
very long pumpout in a vacuum rig may work. It will take a
couple of days but it may get you up and running and may
even save you buying a replacement insulator.

Disclaimer: I take no responsibility for people not
applying common sense to any of the procedures descibed. If
you do not have good technical experience and skills you
are likely to cause further damage.

Good luck,
Ron

On Wed, 6 Feb 2002 14:55:44 -0700 "Marti, Jordi"
{jordi.marti-at-honeywell.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello!
}
} We have a Hitachi H800 TEM (200 Kev,LaB6) and a few days ago we started having problems with the HV. Static discharges
} in the gun area (even at 75 KeV) were traced back to the failed insulator. Hitachi is trying to get us new one, but they
} tell me it could take several months since this is normally not a stock item. If any body out there has one that they
} are willing to part with (for money) or if any body has other suggestions I would really appreciate your input.
}
} Thanks
}
} Jordi Marti
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu Feb 7 06:12:30 2002



From: best.defense101-at-laposte.net
Date: Thu, 07 Feb 2002 04:58:50 -0700
Subject: Thank You For Your Interest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Start Your Investigation Today! Uncover The TRUTH About Anyone!

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---------------------------------------------------------------------------



From daemon Thu Feb 7 07:36:18 2002



From: Windland, Mark J (MN14) :      Mark.Windland-at-honeywell.com
Date: Thu, 7 Feb 2002 07:32:30 -0600
Subject: EDS system pricing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have blown the window on our old Noran EDS detector. I would like to
find out an approximate price on a new system within about 10k. We would
like to replace the old one. Before we get the formal quotes, I though a
few of you that have recently purchased systems could give me a ballpark
price.
Thanks,

Mark Windland
Honeywell
Minneapolis, Minnesota
763-954-2845



From daemon Thu Feb 7 07:37:06 2002



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Thu, 7 Feb 2002 08:31:43 -0500 (EST)
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Tom:

I read Ms Wang's message, thought the same thoughts you articulated here,
and deleted the message. I think that we as a group should agree not to
do homework for students. Thanks for sharing your thoughts with us and
raising the issue.

Don


On Wed, 6 Feb 2002, Tom Phillips wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I think your instructor's hope would be that you figured out the
} answers to class problems on your own. Asking an expert in the field
} and then simply regurgitating that information is a worthless
} exercise. If you are going to invest the time and money required to
} earn a degree, you might want to try to learn something along the
} way. I am a big supporter of listservers but hate to see them used
} in this way.
}
}
} }
} }
} } To whom it may concern:
} }
} } I am an undergraduate student enrolled at the Georgia Institute of
} } Technology.
} } I am in a biomedical engineering class, and we have a problem that we must
} } solve involving electron microscopes. I have a few questions that I hope will
} } get answered ASAP:
} }
} } 1. What are the advantages and disadvantages in using electrons for
} } microscopy
} } rather than light?
} } 2. Does the wavelength of the electrons have anythign to do with the spatial
} } resolution that the microscope produces in the final picture?
} } 3. What is temporal resolution and how is it produced in the electron
} } microscope?
} }
} } Thank you for your time. I greatly appreciate your efforts in helping me
} } understand more of this subject.
} }
} } Sincerely,
} } Jenny Wang
} }
} } -------------------------------------------------
} } Sent through Cyberbuzz- A Server for the Students
} } http://cyberbuzz.gatech.edu/
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}
}

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Thu Feb 7 08:22:09 2002



From: Visitec-at-t-online.de (Martin Klein)
Date: Thu, 7 Feb 2002 15:08:09 +0100
Subject: AW: Ceramic Insulator for Hitachi H800 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jordi,

We had the same problem with the ceramic insulator (sounds like a law of
nature...). A big help in our case was a dentist. He has much experience in
cleaning ceramic and the perfect tools for doing this job. So my advice is
to bring the part to be cleaned to a dentist in your neighborhood.

Best regards
Martin

VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen/Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: mklein-at-visitec-em.de
WWW: http://www.visitec-em.de
++++ Home of the world`s largest SEM ++++



-----Ursprüngliche Nachricht-----
Von: rdoole-at-materials.ox.ac.uk [mailto:rdoole-at-materials.ox.ac.uk]Im
Auftrag von Ron Doole
Gesendet: Donnerstag, 7. Februar 2002 09:49
An: 'Microscopy'
Betreff: Re: Ceramic Insulator for Hitachi H800 TEM


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Jordi,

Sorry to hear about the insulator.

It depends exactly what you mean by 'failed' but we have
had success cleaning ceramic insulators that have been
subjected to tracking of the HT. Even quite deep arcing
tracks have been cleaned out and when returned to service
have worked OK. If they are on the vacuum side they are
just left alone, if they are on a greased joining surface
we ensure that the grease gets well down into the cleaned
track.

I don't know the type of insulator Hitachi use on your
machine but you may be able clean tracks out by polishing
with Al2O3 paste (5um Al2O3 in alcohol). If this does not
work try shot blasting the ceramic with clean Al2O3 beads.
After polishing or blasting blow off with clean N2 (from a
regulated cylinder), clean in several washes of alcohol in
an ultrasonic bath until there is no trace of alumina in
the alcohol and bake out in a clean vacuum oven overnight.
If you cannot get a vacuum oven then heating in air and a
very long pumpout in a vacuum rig may work. It will take a
couple of days but it may get you up and running and may
even save you buying a replacement insulator.

Disclaimer: I take no responsibility for people not
applying common sense to any of the procedures descibed. If
you do not have good technical experience and skills you
are likely to cause further damage.

Good luck,
Ron

On Wed, 6 Feb 2002 14:55:44 -0700 "Marti, Jordi"
{jordi.marti-at-honeywell.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello!
}
} We have a Hitachi H800 TEM (200 Kev,LaB6) and a few days ago we started
having problems with the HV. Static discharges
} in the gun area (even at 75 KeV) were traced back to the failed insulator.
Hitachi is trying to get us new one, but they
} tell me it could take several months since this is normally not a stock
item. If any body out there has one that they
} are willing to part with (for money) or if any body has other suggestions
I would really appreciate your input.
}
} Thanks
}
} Jordi Marti
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk




From daemon Thu Feb 7 08:39:27 2002



From: Lou Bustillos :      lbustillos-at-amalab.com
Date: Thu, 7 Feb 2002 09:52:48 -0500
Subject: Carbon Coater Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I have just been given the approval to purchase a new carbon coater. We are looking for a model that doesn't use a water source. We have an existing Denton 502A that we are going to change to a backup system. It has been a workhorse for us for the past 14 years.(Up and running six hours a day.)

At your lab, what has been a proven carbon coater that will last?

I need to submit three vendors to my boss before he will approve a purchase of a new carbon coater. As you can see I have been out of the loop on carbon coaters for fourteen years so any information will help me tremendously.

Thank you for your help.

Luis Bustillos
AMA Analytical Services, Inc.
lbustillos-at-amalab.com


From daemon Thu Feb 7 08:43:07 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 7 Feb 2002 09:36:14 -0500
Subject: Re: Epson scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Dear Lister:
}
} I have a Epson scanner (Perfection 1200 Photo) with a transparency
} adaptor. Recently, we have some problems when we scan the negative
} films. The pre-scan image looks pretty nice after some adjustment,
} but the final scan image is too dark almost just a piece of black
} stuff.
} Any help will be appreciated.
}
} Thanks
}
} Jinguo Wang
********
I have an Epson Expression 1600 and had the same problem. My
solution was to select "TPU for Pos film" which does not do the
automatic inversion. I then use the Invert function in Photoshop to
reverse the contrast from negative to positive. It works beautifully.
Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Feb 7 09:00:58 2002



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Thu, 7 Feb 2002 09:54:57 -0500 (EST)
Subject: Polaroid Sprintscan 45 Ultra film holders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



About a year ago there was a valuable discussion of negative scanners.
One concern raised about the Polaroid Sprintscan 45 Ultra was the absence
of a film holder for 3 1/4 x 4 1/4 film. A message was sent that said
that John Warren at Polaroid sent several of you free negative holders.

Last year I purchased this scanner and included a 3 1/4 x 4 1/4 negative
holder on the P.O., and have been waiting for the holder. Polaroid told
my sales rep that such a holder never existed and that John Warren no
longer worked a polaroid.

I have (and love) the Polaroid scanner, but am frustrated about not having
the appropriate film holder. Have any of you received this item, does it
have a part number, and how did you come to have it?

Any help would be appreciated.

Thanks,

Don

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Thu Feb 7 10:05:43 2002



From: Lou Ross :      RossLM-at-missouri.edu
Date: Thu, 7 Feb 2002 09:59:46 -0600
Subject: Microbeam Analysis Society information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

The Microbeam Analysis Society's home page has a new url,
http://www.microbeamanalysis.org. We apologize for this inconvience and I
want to thank John Mansfield for his prompt attention in establishing our
new domain name. If you are a subscriber to the MAS listeserver, John
autimatically changed your subscription to the new address at
microprobe-at-microbeamanalysis.org.

Also, the MAS membership email service (masmembership-at-excite.com ) was
interrupted for a few weeks in January but is fully functional again.

Lou Ross
MAS Membership Services
masmembership-at-excite.com
1-800-4MASMEM (1-800-462-7636)
www.microbeamanalysis.org


From daemon Thu Feb 7 10:40:49 2002



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 07 Feb 2002 10:45:01 -0500
Subject: Re: Ask-A-Microscopist:TEM of Ecoli bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Patrick,

Sounds to me like both points 1. and 2. could be explained if the condenser
aperture is out of the column. Its the uppermost adjustable aperture
sticking out of the side of most TEM's. If thats out, you get huge beam
current down the column, big loss of resolution, and its easy to blow out
the sections or support films.

Or even if the condenser aperture is in, if the next adjustable aperture
down the column, the objective aperture, is out, that also could result in
blown out films, and loss of contrast in the image.

As for the German, best to find someone there who can give you a few
pointers - in English,

Good luck,

Gib

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/

} Email: pjfenneran-at-msn.com
} Name: Patrick Fenneran
}
} Organization: Florida Institute of Technology
}
} Education: Graduate College
}
} Location: Melbourne, Florida
}
} Question: I am using an Zeiss 900m TEM looking at E.coli and have the
} following questions:
}
} 1. The formvar/carbon grids keep on getting blown out, like there is
} too much power or the beam is too concentrated.
}
} 2. When I am taking pictures, the bacteria do not have resolution,
} which adjustments should I make?
}
} 3. Do you know of any paperwork that can be obtained that explains
} the operation the components of the machine, the only book I have is
} the one that came with the machine and it is partly in German.
}




From daemon Thu Feb 7 10:47:43 2002



From: Comstock, Robert J. :      comstorj-at-westinghouse.com
Date: Thu, 7 Feb 2002 11:42:38 -0500
Subject: Database/image analysis for digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have progressed during the past few years to where the majority of our
optical and SEM images are digital rather than Polaroid prints. In addition,
we also scan TEM negatives and store them digitally. I am interested in
some recommendations for software that can store the images in a database so
they can be easily retrieved by keywords and also software for image
analysis (e.g., particle size, image analysis, etc.) What options are
available that people have experience with. I'd be interested in hearing
both pro and con.

Thanks,

Bob Comstock
Westinghouse Electric Co.
Pittsburgh, PA 15235


From daemon Thu Feb 7 11:04:23 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 7 Feb 2002 08:58:23 -0800 (PST)
Subject: Re: Ask-A-Microscopist:TEM of Ecoli bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Were the bacteria fixed and stained? Did you carbon coat your formvar
grids before use? What mesh size grids are you using? I have no
information on that particular microscope, but if you have never used any
TEM, you normally use a spread weak beam rather than a concentrated one
for imaging (spread the beam with the condensor lense). Electron
microscopes work best in the dark.

We have some procedures on our website you may wish to browse:
http://biology.berkeley.edu/EML

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Thu, 7 Feb 2002 pjfenneran-at-msn.com wrote:
} 1. The formvar/carbon grids keep on getting blown out, like there is
} too much power or the beam is too concentrated.
}
} 2. When I am taking pictures, the bacteria do not have resolution,
} which adjustments should I make?
}
} 3. Do you know of any paperwork that can be obtained that explains
} the operation the components of the machine, the only book I have is
} the one that came with the machine and it is partly in German.
}
}
}
} ---------------------------------------------------------------------------
}



From daemon Thu Feb 7 11:08:37 2002



From: tartenon-at-netscape.net
Date: Thu, 07 Feb 2002 12:02:19 -0500
Subject: RE: RE: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry You're Right I miss type the numbers it really is 3.2 Megapixels where 2048 X 1536 = 3,145,728 (3.2 Megapixels) I'm using Nikon 995 and I can Photograph Bright, Fluorescence Images My Understanding is that you can increase that resolution using Interpolation, but the real resolution of the image will be 3.2 Megapixels with the Nikon 995. I do not belive Olympus has 4 Megapixels non-interpolated (nor any available digital camera)

Regards

Tina Carvalho {tina-at-pbrc.hawaii.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




__________________________________________________________________
Your favorite stores, helpful shopping tools and great gift ideas. Experience the convenience of buying online with Shop-at-Netscape! http://shopnow.netscape.com/

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From daemon Thu Feb 7 12:18:51 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 7 Feb 2002 12:02:33 -0700
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with Don and Tom.


Donald Lovett wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Tom:
}
} I read Ms Wang's message, thought the same thoughts you articulated here,
} and deleted the message. I think that we as a group should agree not to
} do homework for students. Thanks for sharing your thoughts with us and
} raising the issue.
}
} Don
}
} On Wed, 6 Feb 2002, Tom Phillips wrote:
}
}
} } I think your instructor's hope would be that you figured out the
} } answers to class problems on your own. Asking an expert in the field
} } and then simply regurgitating that information is a worthless
} } exercise. If you are going to invest the time and money required to
} } earn a degree, you might want to try to learn something along the
} } way. I am a big supporter of listservers but hate to see them used
} } in this way.
} }
} } }
} } } To whom it may concern:
} } }
} } } I am an undergraduate student enrolled at the Georgia Institute of
} } } Technology.
} } } I am in a biomedical engineering class, and we have a problem that we must
} } } solve involving electron microscopes. I have a few questions that I hope will
} } } get answered ASAP:
} } }
} } } 1. What are the advantages and disadvantages in using electrons for
} } } microscopy
} } } rather than light?
} } } 2. Does the wavelength of the electrons have anythign to do with the spatial
} } } resolution that the microscope produces in the final picture?
} } } 3. What is temporal resolution and how is it produced in the electron
} } } microscope?
} } }
} } } Thank you for your time. I greatly appreciate your efforts in helping me
} } } understand more of this subject.
} } }
} } } Sincerely,
} } } Jenny Wang
} } }
} } } -------------------------------------------------
} } } Sent through Cyberbuzz- A Server for the Students
} } } http://cyberbuzz.gatech.edu/
} }
} } --
} } Thomas E. Phillips, Ph.D.
} } Associate Professor of Biological Sciences
} } Director, Molecular Cytology Core Facility
} }
} } 3 Tucker Hall
} } Division of Biological Sciences
} } University of Missouri
} } Columbia, MO 65211-7400
} } (573)-882-4712 (voice)
} } (573)-882-0123 (fax)
} }
} }
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-tcnj.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} P.O. Box 7718 fax: (609) 637-5118
} The College of New Jersey
} Ewing, NJ 08628-0718

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




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Hello Listers,

While I agree with what has been said so far in that nobody should do
somebody else's work, especially not homework for students, I am wondering
if that is not being interpreted a bit too narrowly here. After all, this
listserver **IS** a great resource and the students show some initiative in
finding and posting to it. Wouldn't it be better to help the students find
the information they are after rather than denying help? Something like:
"These are very interesting questions, and there are many good books about
this issue. Try reading any one of these books (...) or talk to anyone who
is doing electron microscopy."

Then again, these are my thoughts, and if you disagree, please send me an
email.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Donald Lovett [mailto:lovett-at-tcnj.edu]
Sent: Thursday, February 07, 2002 6:32 AM
To: Tom Phillips
Cc: "gtg990a-at-prism.gatech.edu"-at-sparc5.microscopy.com;
Microscopy-at-sparc5.microscopy.com



Tom:

I read Ms Wang's message, thought the same thoughts you articulated here,
and deleted the message. I think that we as a group should agree not to
do homework for students. Thanks for sharing your thoughts with us and
raising the issue.

Don


On Wed, 6 Feb 2002, Tom Phillips wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I think your instructor's hope would be that you figured out the
} answers to class problems on your own. Asking an expert in the field
} and then simply regurgitating that information is a worthless
} exercise. If you are going to invest the time and money required to
} earn a degree, you might want to try to learn something along the
} way. I am a big supporter of listservers but hate to see them used
} in this way.
}
}
} }
} }
} } To whom it may concern:
} }
} } I am an undergraduate student enrolled at the Georgia Institute of
} } Technology.
} } I am in a biomedical engineering class, and we have a problem that we
must
} } solve involving electron microscopes. I have a few questions that I hope
will
} } get answered ASAP:
} }
} } 1. What are the advantages and disadvantages in using electrons for
} } microscopy
} } rather than light?
} } 2. Does the wavelength of the electrons have anythign to do with the
spatial
} } resolution that the microscope produces in the final picture?
} } 3. What is temporal resolution and how is it produced in the electron
} } microscope?
} }
} } Thank you for your time. I greatly appreciate your efforts in helping me
} } understand more of this subject.
} }
} } Sincerely,
} } Jenny Wang
} }
} } -------------------------------------------------
} } Sent through Cyberbuzz- A Server for the Students
} } http://cyberbuzz.gatech.edu/
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}
}

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Thu Feb 7 14:03:35 2002



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Thu, 07 Feb 2002 13:47:36 -0800
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
I would like to find a distributor for Cargille Laser Liquids (series
5610). I would like to do some experiments involving immersion oils of
varied RI in combination with mountants of differing RI in a manner similar
to the method employed in:

Scalettar, Swedlow, Sedat & Agard. 1996. Dispersion, abberation and
deconvolution in multi-wavelength fluorescence images. Journal of
Microscopy. 182:1, 50-60.

Any advice regarding sources of reagents and techniques to adjust the RI of
immersion media appropriate for LSCM/MP is welcome. Thanks in advance.
-Karl G.

_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



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Bob:
You're likely to get a ton of vendor responses on this one, but I'll throw
in a couple of general thoughts.

Disclaimer: the commercial products mentioned here are EXAMPLES. This use
does not constitute any endorsement by myself or Dow Chemical or imply
anything about the quality or applicability of the product. It also does
not imply anything about the competitive products that are NOT mentioned.

First off, for image analysis, you need to keep ImageJ
(http://rsb.info.nih.gov/ij/) in mind. It is public domain, is fully
customizable (using Java language) has a wide range of users/developers
around the world and works nicely. The user community is doing a lot of
cool stuff, much of which gets back onto the ImageJ download page and is
available for everyone else. This is the next generation of NIH Image, but
it is now a Java app and runs well on MacOS, Windows, UNIX, Linux and any
other OS that supports Java. You are likely to want something commercial
for your "turnkey" stuff, like Image Pro+ (http://www.mediacy.com/), but
ImageJ is a great tool to keep handy.

As for database/archive, I encourage you to do some math:
How many images do you collect per year?
How much additional information do you need to keep with those images in
order for them to be useful?
How many times do you need those images back after the project is complete?
How long do you want to keep the images in on-line storage?
How much on-line storage are you able to afford?
Who will be administering the database and how much will that administration
cost?

We deal with the issue by using our LIMS project number, then burning a
CD(s) with all the images related to that project so that we can pull out
the CD to retrieve the images. We leave the images on the network file
server until the project is complete, then burn the CD and delete the
on-line files.

If we have "definitive" or reference images, we keep those in on-line
storage. These are the ones that really need to be part of a database.

One tool that has been useful is Thumbs+ (http://www.cerious.com/). We can
make an image directory of the project CDs, then browse that to see if we
can find the image we want. Thumbs+ is really aimed at a "smaller"
operation of just a few people. One company that I know of with a product
for maintaining a large-scale database is Advanced Database Systems
(http://www.adsdb.com/), but there are others out there as well. Cost is
proportional to scale!

Bill
William A. Heeschen, Ph.D.
The Dow Chemical Company
Microscopy, Digital Imaging
1897 Bldg, E-84 / 2040 Bldg, 1330
waheeschen-at-dow.com

-----Original Message-----
} From: Comstock, Robert J. [mailto:comstorj-at-westinghouse.com]
Sent: Thursday, February 07, 2002 11:43 AM
To: MicroscopyListserver (E-mail)


We have progressed during the past few years to where the majority of our
optical and SEM images are digital rather than Polaroid prints. In addition,
we also scan TEM negatives and store them digitally. I am interested in
some recommendations for software that can store the images in a database so
they can be easily retrieved by keywords and also software for image
analysis (e.g., particle size, image analysis, etc.) What options are
available that people have experience with. I'd be interested in hearing
both pro and con.

Thanks,

Bob Comstock
Westinghouse Electric Co.
Pittsburgh, PA 15235


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Don:

I'm afraid I disagree with you and Tom. Part of a scientist's training is to learn
how to identify and use resources. And we (collectively) are a resource that I
believe should be made available to all who can benefit. Of course, that leaves open

the question of whether the student benefits more by working things out in isolation,

or by seeking guidance from experts and either really understanding the answers or
(hopefully not) merely regurgitating them by rote. One hopes that the intelligent
student will reject the latter course.

Mike

Donald Lovett wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Tom:
}
} I read Ms Wang's message, thought the same thoughts you articulated here,
} and deleted the message. I think that we as a group should agree not to
} do homework for students. Thanks for sharing your thoughts with us and
} raising the issue.
}
} Don
}
} On Wed, 6 Feb 2002, Tom Phillips wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I think your instructor's hope would be that you figured out the
} } answers to class problems on your own. Asking an expert in the field
} } and then simply regurgitating that information is a worthless
} } exercise. If you are going to invest the time and money required to
} } earn a degree, you might want to try to learn something along the
} } way. I am a big supporter of listservers but hate to see them used
} } in this way.
} }
} }
} } }
} } }
} } } To whom it may concern:
} } }
} } } I am an undergraduate student enrolled at the Georgia Institute of
} } } Technology.
} } } I am in a biomedical engineering class, and we have a problem that we must
} } } solve involving electron microscopes. I have a few questions that I hope will
} } } get answered ASAP:
} } }
} } } 1. What are the advantages and disadvantages in using electrons for
} } } microscopy
} } } rather than light?
} } } 2. Does the wavelength of the electrons have anythign to do with the spatial
} } } resolution that the microscope produces in the final picture?
} } } 3. What is temporal resolution and how is it produced in the electron
} } } microscope?
} } }
} } } Thank you for your time. I greatly appreciate your efforts in helping me
} } } understand more of this subject.
} } }
} } } Sincerely,
} } } Jenny Wang
} } }
} } } -------------------------------------------------
} } } Sent through Cyberbuzz- A Server for the Students
} } } http://cyberbuzz.gatech.edu/
} }
} } --
} } Thomas E. Phillips, Ph.D.
} } Associate Professor of Biological Sciences
} } Director, Molecular Cytology Core Facility
} }
} } 3 Tucker Hall
} } Division of Biological Sciences
} } University of Missouri
} } Columbia, MO 65211-7400
} } (573)-882-4712 (voice)
} } (573)-882-0123 (fax)
} }
} }
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-tcnj.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} P.O. Box 7718 fax: (609) 637-5118
} The College of New Jersey
} Ewing, NJ 08628-0718



From daemon Thu Feb 7 18:57:45 2002



From: DrJohnRuss-at-aol.com
Date: Thu, 7 Feb 2002 19:50:12 EST
Subject: Re: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 2/7/02 12:22:59 PM,
tartenon-at-netscape.net-at-sparc5.microscopy.com writes:

} My Understanding is that you can increase that resolution using
Interpolation,
} but the real resolution of the image will be 3.2 Megapixels with the Nikon
} 995. I do not belive Olympus has 4 Megapixels non-interpolated (nor any
} available digital camera)

It's really a lot more complicated than that. The actual spatial resolution
of digital cameras is not simply the number of transistors on the chip (and
in that regard I have the new Sony DSC-f707 which has 5 megapixels on the
chip and produces awesome images, as compared to my Nikon 995). The problem
is that the single chip cameras use an array of filters to expose some
transistors to red, green and blue light. In order to get the image that is
stored, they use interpolation to fill in the color information where it was
not directly measured. The filters have broad wavelength coverage, and the
interpolation schemes are pretty good (lots of patents in that area). But by
direct measurement based on the Fourier power spectra none of these cameras
has a spatial resolution that approaches the value you would expect based on
the number of pixels in the stored image (which is usually the same as the
number of transistors, except for Fuji who save images that have even more
than that). For the Nikon and Sony cameras it is typical to find that the
actual resolution elements across an image - beyond which you have empty
magnification - number about 2/3 of the number of pixels. So a camera with
2000 transistors on each line (e.g., 2000 x 3000 = 6 megapixels) would
probably measure as having about 1300-1350 elements of resolution (in other
words, if you reduced the image to that size you would not really be
discarding any information, and any enlargement beyond that size is empty).

The spatial resolution of the 5 M-pixel Sony is not as good as a 35 mm film
camera, but it is extremely good all the same and certainly represents the
best quality available now (and probably good enough for a great many
applications) provided you store the image as uncompressed tif and don't
throw away the important details by allowing the camera to use jpeg
compression (which all of these cameras will do by default, to save memory).
The tonal resolution - range of brightness values from dark to bright - is
much more problematic. I have had good experience using high end consumer
digital cameras for bright field microsopy, but they don't begin to have
enough range for darkfield or fluorescence work. Remember that 8 bits is 256
grey levels, and even the cameras with internal 10 bits or more only produce
about 8 bits on output because of the conversion from a linear detector to a
film-like log output. Film easily covers several thousand discernible
brightness steps, which would require a 12 bit or more range. That's why
cooled cameras are used for these applications, and why the tiny chips used
in consumer cameras won't work (the small transistors simply can't hold
enough electrons to give that kind of dynamic range).

If you want to compare consumer type cameras, there is a wealth of unbiased
information available online at {http://www.dpreview.com/reviews/specs.asp} .
The discussion is centered on more typical photographic applications but
there are comparative images, and a lot of info.

Digital cameras are great, and they save a lot of time and money. But don't
expect an inexpensive consumer type camera to cover all applications.


From daemon Thu Feb 7 20:32:43 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 07 Feb 2002 18:28:04 -0800
Subject: RE: RE: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pixera 600-series digital camera produce non-interpolated
honest/real 5.8M pixels, RGB.

gary g.


At 09:02 AM 2/7/2002, you wrote:

} Sorry You're Right I miss type the numbers it really is 3.2 Megapixels
} where 2048 X 1536 = 3,145,728 (3.2 Megapixels) I'm using Nikon 995 and I
} can Photograph Bright, Fluorescence Images My Understanding is that you
} can increase that resolution using Interpolation, but the real resolution
} of the image will be 3.2 Megapixels with the Nikon 995. I do not belive
} Olympus has 4 Megapixels non-interpolated (nor any available digital camera)




From daemon Thu Feb 7 21:41:53 2002



From: Beauregard :      beaurega-at-westol.com
Date: Thu, 07 Feb 2002 22:28:19 -0500
Subject: Re: Polaroid Sprintscan 45 Ultra film holders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Don,

Polaroid will not deliver the holder insert. Period. They did make a few
of them. I saw a metal one, photocopied it and measured it.
I have the insert spec's but not at home here. I could scan it and post it
on my web page.
The thing is only a thin but sturdy piece of sheet metal with rounded edges
that has two slots to fit the two pegs in the 4X5 holder. The holder is
bent or stamped to be slightly recessed, to equal the thickness of a sheet
of film and the recess is the size of a sheet of film. This recess depth
matches the rubberized surface of the regular 4X5 holder. You can make one
from copper sheet stock or brass shim. You would make the outer thin and
slotted piece the thickness of your film. Then solder on a thin piece of
copper below the first piece to hold the film 'recessed'. I bought the
copper material but then it did this:

} From a previous email:
I have a Sprintscan 45i using binuscan as a driver 1.0.3 (wish I didn't).
I could not get Polaroid to furnish any type of holder for 3.25 by 4 inch
cut film, like SO-163. I put the film in sideways and use that. That
raises questions about the focal plane of the film and it bending in the
holder. These are minimal problems for me. However sometimes when the
film is dried, it can be bent. So, I also have my own economy fix that you
can build yourself.

Materials needed:
1 or 2 sheets of the cardboard that come with Kodak SO-163 film.
An old exposed sheet or a new yellow sheet of SO-163 film.
One clear sheet of developed clear and colorless SO-163 film.
20 inches of double back tape.
A roll of 3M Scotchbrand® magic mending tape.
One pair of scissors.
Access to a paper cutter, optional.
One 6 inch ruler with metric millimeters on it.
One Sprintscan 45i 4X5 metal film holder.
One magic marker to blacken all surfaces of the cardboard above (optional).

Cut one sheet of the cardboard so that it is square, 93 mm long and 36 mm
high. It should fit snuggly in the opening of the film holder towards the
locking nut or screw end. Once fitted, place a strip of 3M mending tape on
the length of it so that only half of the tape is pressed onto the 93 mm
long side by the opening for the film. Fold the tape over tightly, no
wrinkles, and press the rest of the tape unto the opposite side of the
blackened cardboard. This 93 mm edge will not fray from now on. Cut off
any excess tape overhanging the ends. Take your colored film sheet and
cut two pieces from the two good 4 inch straight edge sides. One piece
will be "4 inches" ( actually 3 and 7/8ths) by 27 mm. The other will be "4
inches" by 12 mm. Take a piece of scrap 3 1/4 X 4" TEM film with an image
on it and decide how much viewing area you are willing to sacrifice or lose
at the edge of the micrograph that will go up against the cardboard side.
1-2 mm should be about right for a Philips CM12 piece of SO-163 3 ¼ X 4"
cut film.

Place a 4 inch piece of double back tape on the back of the 27 mm wide
piece so that it is flush against the 4 inch edge of it. Place it on the
cardboard so that this 4 inch edge is recessed 1-2 mm from the 4 inch edge
of the cardboard and centered so that 2-3 mm is overhanging the ends of the
cardboard. Repeat this with the 12 mm piece and place it exactly on top of
the first colored film piece. You now have a piece of cardboard with two
pieces of centered colored film taped to it but inset 1-2 mm. The
overhanging film edges will support this template holder in the Polaroid
4x5 holder, when installed.

(You can install more cardboard on the bottom of the first cardboard piece,
if you want the holder to be more rigid.)

Take the 3.25 inch edge of the scrap colorless or developed film and cut a
piece off 3 ¼ inches by 10 mm. Using double backed tape, install the tape
so that it's length covers all 3.25 inches and it is set back 1-2 mm from
the straight 3.25 inch edge of this colorless film piece. Turn it over,
center it lengthwise over the dual stack of film and so that the colorless
film's 3.25 inch edge is flush with the edge of the cardboard, not the
colored dual film pieces. Press it into place on top of the colored film
stack. You have now created a two film thick slot to insert your next TEM
film into that you want to scan and it will be automatically aligned. The
top piece is colorless to provide a view of how you are inserting the
negative being mounted.

One job needs to be done. There is a peg on the 4x5 holder that your film
to be scanned will rest up against. There is another one over by the
locking mechanism. Cut the edge of the new template you made so that it
has a notch that lines up with the peg nearest the locking screw. Press
the ends of the template down onto the holder making sure it is square in
the holder. You should now have an opening that is about 93 mm by 76 mm.
That will be the area of your film that will be scanned. You will notice
that the white or blackened cardboard is below the edge of the metal
holder. Put a few pieces of mending tape around the template to hold it in
place permanently and to keep it from sliding around.

To load the film holder:
Slide the new film into the slot so that it is above the white cardboard
and below the clear film piece. Slide the rest of the film up until it
hits the one peg nearest the hinge. Square up the film if needed. Close
the film holder. The film will be centered in the holder and flat all
around the edges.
The best part is this holder is FREE and only should take an hour or two to
make.

My Polaroid scanner lasted 1½ years and is now defunct. When I turn it on,
it moves the holder in and out continuously. It never initializes. It's
not my favorite piece of equipment. I use a Powerlook III and it's much
faster to scan with it. It does not have the OD range and resolution is
worse but it didn't cost $7000. My customers don't complain.
Use the Fuji setting for low constrast negs. Use regular transmission on
high contrast negs. and invert.

Info on ordering UMAX scanner PLASTIC cut film holders that won't scratch
the glass on your flat bed:
Umax Phone: Area 510 - 651 - 4000 ext 3038
Parts are ordered from Fremont, Calf. only.

#SKIT-29002 PKG of 3 4" by 5" plastic cut film holders. $39.99

??? PKG of 5 2.25" by 3.25" holder
$49.99
These holders can be routed out to make a 3 1/4 X 4" holder. It takes some
skill to do this.
Placing the 3x4 film in sideways in a 4x5 holder works just fine and that's
what I use.

Paul Beauregard
Sr. Research Associate
PPG Industries
Monroeville, PA 15146




} Last year I purchased this scanner and included a 3 1/4 x 4 1/4 negative
} holder on the P.O., and have been waiting for the holder. Polaroid told
} my sales rep that such a holder never existed and that John Warren no
} longer worked a polaroid.
}
} }
} Any help would be appreciated.
}
} Thanks,
}
} Don
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-tcnj.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} P.O. Box 7718 fax: (609) 637-5118
} The College of New Jersey
} Ewing, NJ 08628-0718
}
}
}
}
}
}

} Last year I purchased this scanner and included a 3 1/4 x 4 1/4 negative
} holder on the P.O., and have been waiting for the holder. Polaroid told
} my sales rep that such a holder never existed and that John Warren no
} longer worked a polaroid.
}
} I have (and love) the Polaroid scanner, but am frustrated about not having
} the appropriate film holder. Have any of you received this item, does it
} have a part number, and how did you come to have it?
}
} Any help would be appreciated.
}
} Thanks,
}
} Don
}




From daemon Thu Feb 7 23:44:00 2002



From: gtg990a-at-prism.gatech.edu
Date: Fri, 08 Feb 2002 00:36:56 -0500 (EST)
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I assure all of you who responded to me that I will NOT be just regurgitating
the information you have sent me. All the information was greatly appreciated,
and hopefully I can understand it all to apply to my assignment. This is NOT
just a homework assignment, and there is still much more that I am required to
research and think about and discuss with my group before producing a final
solution. The goal of our class is to learn how to research using different
types of sources, and one of the ways we are told, when one has a limited
amount of time to learn something, is to ask others who are more knowledgeable
in the subject, professionals like yourselves. So thank you for your time for
those who were kind enough to help me.
Sincerely,
Jenny

Quoting Michael O'Keefe {MAOKeefe-at-lbl.gov} :

} Don:
}
} I'm afraid I disagree with you and Tom. Part of a scientist's training
} is to learn
} how to identify and use resources. And we (collectively) are a resource
} that I
} believe should be made available to all who can benefit. Of course,
} that leaves open
} the question of whether the student benefits more by working things out
} in isolation,
} or by seeking guidance from experts and either really understanding the
} answers or
} (hopefully not) merely regurgitating them by rote. One hopes that the
} intelligent
} student will reject the latter course.
}
} Mike
}
} Donald Lovett wrote:
}
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} } Tom:
} }
} } I read Ms Wang's message, thought the same thoughts you articulated
} here,
} } and deleted the message. I think that we as a group should agree not
} to
} } do homework for students. Thanks for sharing your thoughts with us
} and
} } raising the issue.
} }
} } Don
} }
} } On Wed, 6 Feb 2002, Tom Phillips wrote:
} }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } I think your instructor's hope would be that you figured out the
} } } answers to class problems on your own. Asking an expert in the
} field
} } } and then simply regurgitating that information is a worthless
} } } exercise. If you are going to invest the time and money required
} to
} } } earn a degree, you might want to try to learn something along the
} } } way. I am a big supporter of listservers but hate to see them
} used
} } } in this way.
} } }
} } }
} } } }
} } } }
} } } } To whom it may concern:
} } } }
} } } } I am an undergraduate student enrolled at the Georgia Institute
} of
} } } } Technology.
} } } } I am in a biomedical engineering class, and we have a problem that
} we must
} } } } solve involving electron microscopes. I have a few questions that
} I hope will
} } } } get answered ASAP:
} } } }
} } } } 1. What are the advantages and disadvantages in using electrons
} for
} } } } microscopy
} } } } rather than light?
} } } } 2. Does the wavelength of the electrons have anythign to do with
} the spatial
} } } } resolution that the microscope produces in the final picture?
} } } } 3. What is temporal resolution and how is it produced in the
} electron
} } } } microscope?
} } } }
} } } } Thank you for your time. I greatly appreciate your efforts in
} helping me
} } } } understand more of this subject.
} } } }
} } } } Sincerely,
} } } } Jenny Wang
} } } }
} } } } -------------------------------------------------
} } } } Sent through Cyberbuzz- A Server for the Students
} } } } http://cyberbuzz.gatech.edu/
} } }
} } } --
} } } Thomas E. Phillips, Ph.D.
} } } Associate Professor of Biological Sciences
} } } Director, Molecular Cytology Core Facility
} } }
} } } 3 Tucker Hall
} } } Division of Biological Sciences
} } } University of Missouri
} } } Columbia, MO 65211-7400
} } } (573)-882-4712 (voice)
} } } (573)-882-0123 (fax)
} } }
} } }
} }
} }
} ______________________________________________________________________
} } Donald L. Lovett e-mail: lovett-at-tcnj.edu
} } Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} } P.O. Box 7718 fax: (609) 637-5118
} } The College of New Jersey
} } Ewing, NJ 08628-0718
}
}


-------------------------------------------------
Sent through Cyberbuzz- A Server for the Students
http://cyberbuzz.gatech.edu/


From daemon Fri Feb 8 00:29:37 2002



From: Manuel E. Brito :      manuel-brito-at-aist.go.jp
Date: Fri, 08 Feb 2002 15:38:12 +0900
Subject: Macro-, Micro- and Meso-porous Materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

The deadline for abstract submission for the Microscopy &
Microanalysis 2002 (Quebec City, Canada) is rapidly approaching.
Among the featured Symposia at this year meeting, "Electron
Microscopy of Macro-, Micro- and Meso-porous Materials"
will address the current state of the art.

The deadline for electronic abstract submission is
February 15th. We look forward to seeing you in Quebec City!


Manuel E. Brito, AIST, Japan
Douglas Blum, ORNL, USA Dear Colleagues,


--
_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
National Institute of Advanced
Industrial Science and Technology
Synergy Materials Research Center

Manuel E. Brito, Eng. D.

Moriyama-ku, Nagoya 463-8687 JAPAN
Tel: +81-52-739-0135 Fax: +81-52-739-0136
e-mail: manuel-brito-at-aist.go.jp

_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/




From daemon Fri Feb 8 02:21:44 2002



From: =?ISO-8859-2?Q?Old=F8ich_Benada?= :      benada-at-biomed.cas.cz
Date: Fri, 8 Feb 2002 09:19:31 +0100
Subject: RE: measurement and calibration onthe SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I think you have meant 2160 lines per mm giving 0.46 um between two
adjacent lines (a standard replica grating); 2600 lines per mm should
give .38 um spacing.

Best regard from Prague
Oldrich



On 6 Feb 2002 at 15:30, Dusevich, Vladimir wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} } -----Original Message-----
} } From: Brian Wajdyk [mailto:electronmicroscopist-at-hotmail.com]
} } Sent: Wednesday, February 06, 2002 10:47 AM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: measurement and calibration onthe SEM
} }
} }
} } --------------------------------------------------------------
} } ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } ---------.
} }
} }
} } Fellow Microscopists,
} }
} } SEM has historically been used for metrology of various
} } structures. I can't
} } seem to find much literature about the artifacts associated
} } with this type
} } of measurement. We do use the NIST standards to check the
} } calibration of
} } our equipment, but I haven't characterized how the different
} } beam or sample
} } parameters effect the measurements. What do you do? Has
} } anyone figured out
} } his or her actual accuracy and precision? We have found that
} } we can safely
} } give measurements within +/-5% taking into account most human
} } and equipment
} } errors. This is based on the precision of measurements made of NIST
} } structures, measured the same way, over several years. On
} } the other hand,
} } the smallest structure we can measure on the standard is 2um
} } (line and
} } space. How do you determine at what magnification you will no longer
}
} Cheap grating replicas with 2600 lines per mm give 0.46 um per line,
} and this is not too bad for calibrating 50,000 magnification. I am
} happy I do not need certified standards.
}
} } guarantee the measurement? I see that my MRS-3 from Geller
} } says it's for
} } 10x to 50kX. How do they figure out that the max magnification it is
} } useful? Maybe it as simple as being able to fit the structure on the
}
} Maximum useful magnification is very specimen dependant, especially
} for low voltage and low vacuum modes. Of course, for digital images
} it is possible to check brightness profiles and if they have slopes
} on edges of features, then measure "size" on half height of the slope.
} But I am not aware about publications which dependably justify this kind
} of measurements (manipulations with brightness and contrast and
} specimen tilt could change slopes significantly).
}
} } screen. If that were true, you would expect that the
} } instrument would also
} } be calibrated to a much higher magnification. How high could
} } I say it is
} } accurate to? Can I safely measure a 1000A line assuming no
}
} It depends on resolution for your microscope/specimens and on
} calibration standard you are using. And I think periodic lines with
} spacing 2 um not really good standard to measure feature with
} the size of 0.1 um.
}
} } obvious issues
} } (i.e. drift)? Can anyone educate me more on this topic or
}
} If you have visible drift during single exposure, then something
} wrong with microscope or specimen preparation technique.
}
} } point me to
} } resources?
} }
} }
} } Things that could effect measurements (feel free to add to list):
} } Drift (mechanical / beam)-
}
} exposure time should be small for significant drift.
}
} } Charging (obvious or stretching of image from a slow scan)
} } Magnification (adjusted for each set of lens relays)
}
} Could be eliminated with proper calibration.
}
} } kV (surface vs. subsurface image)
} } Working Distance
}
} Could be eliminated with proper calibration.
}
} } Delineation method (raised vs. depression, materials contrast)
} } Amount of delineation (3D effect)
} } Resolution (near resolution limit of SEM?)
} } Contrast (or lack of, bright / dark line)
} } Edge effect (bright line)
} } Consistency between tools (calibration, etc.)
} } Consistency between methods (SEM, TEM, ellipsometry, AFM, etc.)
} } Operator's eye (where to measure. Measure outside to inside,
} } center to
} } center, out to out, in to in?)
} } Variance in measured layer thickness (topography, sloped
} } profile (i.e. base
} } larger than top))
} } Angle to beam
} } Preparation methods (polish (i.e. smearing), cleave (i.e.
} } pull of soft
} } material), FIB (i.e. angle))
} } Type of algorithm if doing it automatically (i.e. %50 threshold)
}
} Some of the things you have mentioned relate to specimen/experiment,
} to stereology, but not to microscope. For example, if I need to measure
} size of depression without sharp edges, I have to find (or at least to
} declare)right procedure for it's measurements. May be I have to perform
} stereo measurements and define an edge as a place, where a depth of
} depression become equal to 0.1 um (or 10% of total depth, or
} whatever else, depending on a study).
}
} And thank you for your extensive list - it is very helpful for
} observation of the problem. And about additions to your list -
} I think everybody can say something. For example recently I tried
} to measure in ESEM thickness of a layer which, as it turned out,
} was a viscous liquid...
}
} Regards,
}
} Vladimir
}
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}


+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4752347
WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm



From daemon Fri Feb 8 06:18:32 2002



From: Shea Miller :      millers-at-EM.AGR.CA
Date: Fri, 08 Feb 2002 07:14:20 -0500
Subject: help with buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings all;
can anyone provide the formulation for Hank's buffer? I have looked in all of my books (most, but not all of which, admittedly, lean towards plants) and can't find a single reference. It is called for in a protocol for controlling autofluorescence in aldehyde fixed tissue.

thanks in advance
shea


Dr. S. Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal & Oilseed Research Centre
Rm. 2068, K.W. Neatby Bldg.
Central Experimental Farm
Ottawa, Ontario,
Canada K1A 0C6
Phone: (613)759-1760
Fax: (613)759-1701
E-mail: millers-at-em.agr.ca



From daemon Fri Feb 8 06:52:08 2002



From: ÏæÁÕ :      Xianglin_Li-at-student.uml.edu
Date: Fri, 8 Feb 2002 7:47:2 -0500
Subject: Do you have any recommended books?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all;

I have two sets of data to interpreted:
1) X-ray rocking curve data, to analyze the defect (damage) of the wafer surface.
2) XPS data, to trace how the wafer surface composition will change with the increasing of sputtering time.

I need to have some fundamental idea of these two theories to interpreted data. Do you have any recommendations of the textbooks, or other papers I should read?

Any kind of help will be appreciated!

Xianglin Li

Xianglin_Li-at-student.uml.edu



From daemon Fri Feb 8 07:34:32 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Fri, 08 Feb 2002 08:27:27 -0500
Subject: Re: Database/image analysis for digital images

Contents Retrieved from Microscopy Listserver Archives
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I have been quite happy with SIS Analysis package and database. We generate about 10-15k images a year here and it does a nice job managing it all and keeping relevant data with the images. My users may place a copy of the full program on their PC and run the analysis offline hooking directly to the network served db (MS Access based) and a new option will soon allow my users to log in with a web browser and query the db for their data directly- password protected and everything (hit a couple of snags and don't have it worked out yet). All three SEM's capture directly into the db, TEM data is dragged and dropped after capture, and I hope to soon have the LM directly capturing into it as well. I just haven't gotten it installed yet.

Hitachi distributes PCI quartz which seems very similar though I have no experience with it.

On the cheap side there is a program called thumbs+ from Cerious software for simple archiving.

Scott Whittaker
SEM Lab Manager
Smithsonian Institution
PO Box 37012 MRC104
National Museum of Natural History
Washington DC 20560-0104
202-357-1651


} } } "Comstock, Robert J." {comstorj-at-westinghouse.com} 02/07/02 11:42AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We have progressed during the past few years to where the majority of our
optical and SEM images are digital rather than Polaroid prints. In addition,
we also scan TEM negatives and store them digitally. I am interested in
some recommendations for software that can store the images in a database so
they can be easily retrieved by keywords and also software for image
analysis (e.g., particle size, image analysis, etc.) What options are
available that people have experience with. I'd be interested in hearing
both pro and con.

Thanks,

Bob Comstock
Westinghouse Electric Co.
Pittsburgh, PA 15235




From daemon Fri Feb 8 07:48:17 2002



From: Oakley, Jeff :      oakleyj-at-rayovac.com
Date: Fri, 8 Feb 2002 07:42:30 -0600
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm rather embarrassed that some of the members of this listserver were too
narrow minded and/or arrogant to see that Ms. Wang was using this group of
experts as a source, just as one would use a book. And besides, if she were
just going to "regurgitate" the information learned here, wouldn't she just
be doing the same with information pulled from a book?

I suddenly feel dirty somehow... Oh my God... It won't wash off!


Jeff (I'm in for it now) Oakley



} } I think your instructor's hope would be that you figured out the
} } answers to class problems on your own. Asking an expert in the field
} } and then simply regurgitating that information is a worthless
} } exercise. If you are going to invest the time and money required to
} } earn a degree, you might want to try to learn something along the
} } way. I am a big supporter of listservers but hate to see them used
} } in this way.
} }
} } }
} } } To whom it may concern:
} } }
} } } I am an undergraduate student enrolled at the Georgia Institute of
} } } Technology.
} } } I am in a biomedical engineering class, and we have a problem that we
must
} } } solve involving electron microscopes. I have a few questions that I
hope will
} } } get answered ASAP:
} } }
} } } 1. What are the advantages and disadvantages in using electrons for
} } } microscopy
} } } rather than light?
} } } 2. Does the wavelength of the electrons have anythign to do with the
spatial
} } } resolution that the microscope produces in the final picture?
} } } 3. What is temporal resolution and how is it produced in the electron
} } } microscope?
} } }
} } } Thank you for your time. I greatly appreciate your efforts in helping
me
} } } understand more of this subject.
} } }
} } } Sincerely,
} } } Jenny Wang



From daemon Fri Feb 8 08:04:10 2002



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Fri, 1 Jan 1904 09:28:42 -0500
Subject: Undergrads wanted for research

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


NSF REU Program in Nanotechnology at Advanced Materials Processing and
Analysis Center (AMPAC) at
University of Central Florida

Sample Research Projects:
Nanomaterials for coatings, sensors and optics, Nano biology
Solgel, Microemulsion, Laser processing, Mechanical Alloying
Carbon nanotubes, Atomic and Near Atomic Scale Characterization
Nanomaechanics, Focussed Ion Beam in Nanotechnology
Nanospectroscopy using Lasers, Nanostructured TBCs and Polymers

Program Description:
Open to Juniors & Seniors in Fall 2002
Students will work with Faculty in Nanotechnology Projects
Basic concepts in Materials Eng, Physics, Biology, Engineering
Selection: Applicant academic standing, 2 reference letters, statement of
interest

Fellowship: $3000, up to $400 travel, + Accommodation
No of Fellows: 10
Duration: 10 summer weeks ( 20th May - 27th July 2002)
Application Deadline: March 15th 2002
Award Notification: March 25th 2002

For more information contact:

Dr. S. Seal or Karen Glidewell
Room 381, AMPAC, 4000 University Blvd
P.O. Box 162455
UCF, Orlando, Fl 32816
Phone: 407 882 1456 or 823 5277
Fax: 407 882 1462, 823 0208
sseal-at-pegasus.cc.ucf.edu, kglidewe-at-mail.ucf.edu

Visit our Website
http://nanotech.research.ucf.edu/nsf-reu.htm

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Fri Feb 8 09:02:34 2002



From: Jim Quinn :      jquinn-at-www.matscieng.sunysb.edu
Date: Fri, 8 Feb 2002 09:53:12 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id JAA17530
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for Microscopy-at-sparc5.microscopy.com; Fri, 8 Feb 2002 09:53:12 -0500



Don -

College students should not broadcast messages seeking answers
to elementary questions. Additionally, the use of "ASAP"
was a bad idea.

JQuinn

PS: I sent Jenny Wang's message to her Chairperson and UG Program Director.

} From Microscopy-request-at-sparc5.microscopy.com Fri Feb 8 03:44:15 2002
} Date: Thu, 07 Feb 2002 13:47:36 -0800
} From: "Michael O'Keefe" {MAOKeefe-at-lbl.gov}
} Organization: Lawrence Berkeley National Laboratory
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Questions on the Electron Microscope
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Don:
}
} I'm afraid I disagree with you and Tom. Part of a scientist's training is to learn
} how to identify and use resources. And we (collectively) are a resource that I
} believe should be made available to all who can benefit. Of course, that leaves open
}
} the question of whether the student benefits more by working things out in isolation,
}
} or by seeking guidance from experts and either really understanding the answers or
} (hopefully not) merely regurgitating them by rote. One hopes that the intelligent
} student will reject the latter course.
}
} Mike
}
} Donald Lovett wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Tom:
} }
} } I read Ms Wang's message, thought the same thoughts you articulated here,
} } and deleted the message. I think that we as a group should agree not to
} } do homework for students. Thanks for sharing your thoughts with us and
} } raising the issue.
} }
} } Don
} }
} } On Wed, 6 Feb 2002, Tom Phillips wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } I think your instructor's hope would be that you figured out the
} } } answers to class problems on your own. Asking an expert in the field
} } } and then simply regurgitating that information is a worthless
} } } exercise. If you are going to invest the time and money required to
} } } earn a degree, you might want to try to learn something along the
} } } way. I am a big supporter of listservers but hate to see them used
} } } in this way.
} } }
} } }
} } } }
} } } }
} } } } To whom it may concern:
} } } }
} } } } I am an undergraduate student enrolled at the Georgia Institute of
} } } } Technology.
} } } } I am in a biomedical engineering class, and we have a problem that we must
} } } } solve involving electron microscopes. I have a few questions that I hope will
} } } } get answered ASAP:
} } } }
} } } } 1. What are the advantages and disadvantages in using electrons for
} } } } microscopy
} } } } rather than light?
} } } } 2. Does the wavelength of the electrons have anythign to do with the spatial
} } } } resolution that the microscope produces in the final picture?
} } } } 3. What is temporal resolution and how is it produced in the electron
} } } } microscope?
} } } }
} } } } Thank you for your time. I greatly appreciate your efforts in helping me
} } } } understand more of this subject.
} } } }
} } } } Sincerely,
} } } } Jenny Wang
} } } }
} } } } -------------------------------------------------
} } } } Sent through Cyberbuzz- A Server for the Students
} } } } http://cyberbuzz.gatech.edu/
} } }
} } } --
} } } Thomas E. Phillips, Ph.D.
} } } Associate Professor of Biological Sciences
} } } Director, Molecular Cytology Core Facility
} } }
} } } 3 Tucker Hall
} } } Division of Biological Sciences
} } } University of Missouri
} } } Columbia, MO 65211-7400
} } } (573)-882-4712 (voice)
} } } (573)-882-0123 (fax)
} } }
} } }
} }
} } ______________________________________________________________________
} } Donald L. Lovett e-mail: lovett-at-tcnj.edu
} } Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} } P.O. Box 7718 fax: (609) 637-5118
} } The College of New Jersey
} } Ewing, NJ 08628-0718
}
}


From daemon Fri Feb 8 09:18:58 2002



From: DrJohnRuss-at-aol.com
Date: Fri, 8 Feb 2002 10:13:16 EST
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 2/8/02 10:07:03 AM, jquinn-at-www.matscieng.sunysb.edu writes:

} College students should not broadcast messages seeking answers
} to elementary questions.

Some of them do things even worse than that. As the author of a moderately
well known book on image analysis I get several messages each week asking
questions that boil down to something like this:

"I've been asked to report on X. Could you give me a concise answer so I
won't have to read and digest all of the information in your text? Oh, and I
need it by tomorrow.

The only question that is even more annoying is "I can't afford your book.
Would you please send me a copy?"

The art of reading, digesting and combining information from multiple sources
is vital in education. To try to short cut this and get someone else to chew
the food for you and then regurgitate it is not only lazy and dishonest, it
also prevents students from learning to think, which is a more important part
of education than the factual stuff they seem to be dealing with.



From daemon Fri Feb 8 09:26:48 2002



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Fri, 08 Feb 2002 10:21:24 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

While Jenny's questions were rather vague and sounded like she was looking
for an easy answer to an assignment, we should probably give students the
benefit of the doubt. How many times have *we* presented ill-formed
questions when we were not quite sure of what we were asking?

I agree that we should not do a student's assignment for him, but perhaps
we can somewhat more gently steer them to the source of the answers rather
than flame them. I think that if the questions sound inappropriate, we can
make a comment to the effect that we're not going to provide the answer,
but only the source of the info. The ensuing discussion may lead to a
sharpening of the question as the student thinks though what he is trying
to ask. While there are, indeed, students looking for the easy way out, we
need to be careful not to flame the student who framed the question poorly.

Cheers,
Henk Colijn

{...much deleted material...}


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Fri Feb 8 09:31:39 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 8 Feb 2002 09:26:06 -0600
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeff: I am assuming from your rayovac.com you are not an educator.
That doesn't disqualify your opinion but I would be more worried if
other currently teaching academics widely expressed this view.
Learning to look something up in the library is part of the teaching
assignment. Finding the correct information and distilling it is not
trivial. Most experts on the listserver could answer each of those
questions in a few concise sentences. You would be hard pressed to
find any source in a library in which you found the question followed
by the answer. When you read the literature, lots of time you end
up reading additional information on peripheral topics that add to
the learning process. More importantly, students constantly give me
sentences in their papers that are clearly paraphrased from the
literature. I am not suggesting this constitutes plagiarism but it
is often apparent from the sentence that they don't really understand
what it means. They think they do but when I discuss it with them,
they are unable to explain the sophisticated sentence in basic terms.
Students frequently comment in my teaching evaluations that the most
important thing they learned in class was that memorizing and
rephrasing the literature doesn't equate to real understanding. If
the instructor wanted them simply to ask an expert to get the bottom
line answer, why didn't the instructor simply say it in lecture or
give them a handout? Don't you think the instructor knew? Do you
think a bioengineering class was designed to teach web surfing tools.
I don't know if you should feel dirty but I think your batteries need
recharging. Tom Phillips


}
}
} I'm rather embarrassed that some of the members of this listserver were too
} narrow minded and/or arrogant to see that Ms. Wang was using this group of
} experts as a source, just as one would use a book. And besides, if she were
} just going to "regurgitate" the information learned here, wouldn't she just
} be doing the same with information pulled from a book?
}
} I suddenly feel dirty somehow... Oh my God... It won't wash off!
}
}
} Jeff (I'm in for it now) Oakley
}
}
}
} } } I think your instructor's hope would be that you figured out the
} } } answers to class problems on your own. Asking an expert in the field
} } } and then simply regurgitating that information is a worthless
} } } exercise. If you are going to invest the time and money required to
} } } earn a degree, you might want to try to learn something along the
} } } way. I am a big supporter of listservers but hate to see them used
} } } in this way.
} } }
} } } }
} } } } To whom it may concern:
} } } }
} } } } I am an undergraduate student enrolled at the Georgia Institute of
} } } } Technology.
} } } } I am in a biomedical engineering class, and we have a problem that we
} must
} } } } solve involving electron microscopes. I have a few questions that I
} hope will
} } } } get answered ASAP:
} } } }
} } } } 1. What are the advantages and disadvantages in using electrons for
} } } } microscopy
} } } } rather than light?
} } } } 2. Does the wavelength of the electrons have anythign to do with the
} spatial
} } } } resolution that the microscope produces in the final picture?
} } } } 3. What is temporal resolution and how is it produced in the electron
} } } } microscope?
} } } }
} } } } Thank you for your time. I greatly appreciate your efforts in helping
} me
} } } } understand more of this subject.
} } } }
} } } } Sincerely,
} } } } Jenny Wang

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Feb 8 09:34:17 2002



From: Gary M. Easton :      gary.easton-at-scannerscorp.com
Date: Fri, 8 Feb 2002 10:27:56 -0500
Subject: Looking for used EDS Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I am in need of a used but functional EDS detector ported for an ETEC
AUTOSCAN SEM. Preferences being Tracor/Noran, Kevex, PGT or EDAX, T/W if
possible. If anyone has any leads, please feel free to contact me off line.
Thanks in advance.



Gary M. Easton, President
Scanners Corporation
90 Aileron Court, Suite 6
Westminster, Maryland USA 21157
410.857.7633(v)
410.857.7636(f)
www.scannerscorp.com




From daemon Fri Feb 8 09:36:44 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Fri, 08 Feb 2002 16:30:49 +0100
Subject: Re: Database/image analysis for digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

There is a collection of image analysis software available on my
"microscopy and imaging" webpage and also several links to image
databases:

http://ourworld.compuserve.com/homepages/pvosta/pcrimg.htm

Which software environment you choose depends largely on your needs.
Most people need a standard package which is easy to use and there are
several software packages on the market. Do you want to do basic image
analysis or do you ever want to do more complicated analysis on large
datasets, this will influence your choice. Most users "use" imaging
software and don't do a lot of basic image algorithm development
themselves.

Most of the software is available for both Windows and Macintosh, like
the "NIH-Image" family. There is also AnalySIS and ImagePro Plus. For EM
there is specialised software from Gatan and a software package like
KHOROS is also suitable.

There are several image databases available, I believe there are now
several packages available with a web based interface which enables you
to browse the database through a webbrowser.

Best regards,

Peter


} -----Original Message-----
} } From: Comstock, Robert J. [mailto:comstorj-at-westinghouse.com]
} Sent: Thursday, February 07, 2002 11:43 AM
} To: MicroscopyListserver (E-mail)
} Subject: Database/image analysis for digital images
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We have progressed during the past few years to where the majority of our
} optical and SEM images are digital rather than Polaroid prints. In addition,
} we also scan TEM negatives and store them digitally. I am interested in
} some recommendations for software that can store the images in a database so
} they can be easily retrieved by keywords and also software for image
} analysis (e.g., particle size, image analysis, etc.) What options are
} available that people have experience with. I'd be interested in hearing
} both pro and con.
}
} Thanks,
}
} Bob Comstock
} Westinghouse Electric Co.
} Pittsburgh, PA 15235

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621


From daemon Fri Feb 8 09:39:43 2002



From: Curtis Olson :      COlson-at-scpglobal.com
Date: Fri, 08 Feb 2002 08:31:24 -0700
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,

I am new to this listserve so I hesitated to respond, but here it goes. I think a student's questions should be answered in a fashion as you suggest. Mining all resources for information (including media such as this) is a very necessary skill in todays (and definitely tomorrows) world. Whether we agree or not, searching table of contents in the hardcopy library is becoming less and less valuable. Having high school students as children, I have been exposed to a tremendous opportunity for expeditious research covering a broad spectrum of resources using this media. At this time, a combination of hardcopy library and electronic media seems appropriate.

The concern of regurgitation may be moot. After all, from what I have read, this student may very well have the best of intentions and will list this server as her information source. This is an ethical question only she can answer for herself. In addition, it's certainly possible that this is a small fraction of her group's assignment and gleaning the answers to preliminary questions here will only open doors to deeper understanding later.

Obviously, to use a resource such as this to do frequent homework assignments is a mis-use of our time. However, the natural and logical consequences are for the student to deal with when he/she reconciles with the ethical questions and attempts to enter the job market.

Thank you.

Curtis Olson



From daemon Fri Feb 8 09:40:14 2002



From: Bruce Ingber :      bingber-at-srrc.ars.usda.gov
Date: Fri, 08 Feb 2002 09:37:21 -0600
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jenny, you are to be commended on your fine response to the negative comments of some of "my" listserver colleagues. Those of us with 10-25 years of "hand's on experience" in various aspects of microscopy are a valuable asset of knowledge for new students in our field. Likewise, there is much we can hopefully learn your age group. Good luck!




Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
bingber-at-srrc.ars.usda.gov
504-286-4270 phone
504-286-4419 fax



From daemon Fri Feb 8 09:52:35 2002



From: Windland, Mark J (MN14) :      Mark.Windland-at-honeywell.com
Date: Fri, 8 Feb 2002 09:50:35 -0600
Subject: calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We need to purchase a new calibration standard for our SEM that gets us down
to the next level. We need to have accuracy down to 0.010 microns. We have
been using the Geller MRS-3 and now are considering purchasing the MRS-4
traceable standard.
I believe this will give us what we need but I was wondering if there are
other standards out there that are better, the same, worse? I need to hear
from the calibration specialists out there.
Thanks,

Mark Windland
Honeywell
Minneapolis, Minnesota
763-954-2845


From daemon Fri Feb 8 11:08:58 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Fri, 8 Feb 2002 10:45:47 -0600
Subject: Semiconductor Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,

I am looking for some SEM images of semiconductor circuits. Would like to
have a range of magnifications and view angles from those that show whole
die with bond pads and leads to close-ups of circiut elements. Looking for
interesting features and topography. These will be used as guides to build
some 3D models for a SEM animation video I am working on. If anyone can
supply images for this project please send them to me via E-mail.

Thanks for your help!

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu





From daemon Fri Feb 8 11:09:48 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 8 Feb 2002 12:04:35 -0500
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Though I was not in on the discussion, I am going to throw in my
two cents.

When I read the original post, it looked like someone had a test
sheet, or assignment sheet, with those questions on it. The request
appeared to be for direct answers to those questions. It did not
surprise me that this fine collection of knowledge would conclude
that someone was trying to shortcut the leaning process, and
avoid really learning a subject. If the student was going to just
"regurgitate" something from a book, then we have not contributed
to the current decay in the quality of the knowledge, and understanding,
in the students receiving degrees. Those posts that did give excellent
research sources, ignoring the APPARENT shortcut, did well in my
view. If the request was for help in understanding a concept, function,
process, etc., then a more direct answer from an expert becomes
extremely valuable.

I don't think ANYBODY on the list should be embarrassed or ashamed.
Had Ms. Wang requested help in a different manner, she would have
gotten a stream of replies with all sorts of information, and the simple,
fill out the test, answers probably would have been in there, also.

Darrell

"Oakley, Jeff" {oakleyj-at-rayovac.com} on 02/08/2002 08:42:30 AM

To: Microscopy-at-sparc5.microscopy.com
cc:


I'm rather embarrassed that some of the members of this listserver were too
narrow minded and/or arrogant to see that Ms. Wang was using this group of
experts as a source, just as one would use a book. And besides, if she
were
just going to "regurgitate" the information learned here, wouldn't she just
be doing the same with information pulled from a book?

I suddenly feel dirty somehow... Oh my God... It won't wash off!


Jeff (I'm in for it now) Oakley



} } I think your instructor's hope would be that you figured out the
} } answers to class problems on your own. Asking an expert in the field
} } and then simply regurgitating that information is a worthless
} } exercise. If you are going to invest the time and money required to
} } earn a degree, you might want to try to learn something along the
} } way. I am a big supporter of listservers but hate to see them used
} } in this way.
} }
} } }
} } } To whom it may concern:
} } }
} } } I am an undergraduate student enrolled at the Georgia Institute of
} } } Technology.
} } } I am in a biomedical engineering class, and we have a problem that we
must
} } } solve involving electron microscopes. I have a few questions that I
hope will
} } } get answered ASAP:
} } }
} } } 1. What are the advantages and disadvantages in using electrons for
} } } microscopy
} } } rather than light?
} } } 2. Does the wavelength of the electrons have anythign to do with the
spatial
} } } resolution that the microscope produces in the final picture?
} } } 3. What is temporal resolution and how is it produced in the electron
} } } microscope?
} } }
} } } Thank you for your time. I greatly appreciate your efforts in helping
me
} } } understand more of this subject.
} } }
} } } Sincerely,
} } } Jenny Wang







From daemon Fri Feb 8 11:15:22 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 08 Feb 2002 12:11:00 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Roy:

"Beavers, Roy" wrote:

} Gentlemen,
}
} I disagree and find this attitude somewhat surprising coming from educators.
} I took the time to answer her questions and encourage her in her studies. I
} believe this list is just as valuable a resource as any other method she
} could have used. I believe students should feel comfortable in using it in
} the attempt to "learn something along the way".

Her instructor wanted her to research the answer, otherwise he would have
told her the answers (to her very elementary questions) himself. Asking someone
other than her instructor is not research. The way her questions were asked
indicated to me that she was merely repeating questions she had been asked. As
for identifying sources of information, a college-level biology text would have
the answers she wanted. A text book on EM would have the answers. Even a web
site on EM would have the answers. She did not look for any of those, she tried
to get the answers handed to her with no more effort than a e-mail. She probable
still does not know that all of those other resources exist. What will she do if
her server goes down? At my institution, we insist that students look up simple,
straightforward facts for themselves rather than using the faculty as
encyclopeidas. That is what the textbook is for. Once they are in possession of
the facts, we then ask them to use them to solve problems. We don't view
reciting facts to students as higher education.

} Roy Beavers
} Southern Methodist University
} Dept. of Geological Sciences
} Electron Microprobe Lab
} P.O. Box 750395
} Dallas, Tx 75275
} voice: 214-768-2756
} fax: 214-768-2701
} E-mail: rbeavers-at-mail.smu.edu
}
}
}
} -----Original Message-----
} From: Geoff McAuliffe [mailto:mcauliff-at-UMDNJ.EDU]
} Sent: Thursday, February 07, 2002 11:59 AM
} To: Donald Lovett
} Cc: Tom Phillips; Microscopy-at-sparc5.microscopy.com
} Subject: Re: Questions on the Electron Microscope
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I agree with Don and Tom.
}
} Donald Lovett wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Tom:
} }
} } I read Ms Wang's message, thought the same thoughts you articulated here,
} } and deleted the message. I think that we as a group should agree not to
} } do homework for students. Thanks for sharing your thoughts with us and
} } raising the issue.
} }
} } Don
} }
} } On Wed, 6 Feb 2002, Tom Phillips wrote:
} }
} }
} } } I think your instructor's hope would be that you figured out the
} } } answers to class problems on your own. Asking an expert in the field
} } } and then simply regurgitating that information is a worthless
} } } exercise. If you are going to invest the time and money required to
} } } earn a degree, you might want to try to learn something along the
} } } way. I am a big supporter of listservers but hate to see them used
} } } in this way.
} } }
} } } }
} } } } To whom it may concern:
} } } }
} } } } I am an undergraduate student enrolled at the Georgia Institute of
} } } } Technology.
} } } } I am in a biomedical engineering class, and we have a problem that we
} must
} } } } solve involving electron microscopes. I have a few questions that I
} hope will
} } } } get answered ASAP:
} } } }
} } } } 1. What are the advantages and disadvantages in using electrons for
} } } } microscopy
} } } } rather than light?
} } } } 2. Does the wavelength of the electrons have anythign to do with the
} spatial
} } } } resolution that the microscope produces in the final picture?
} } } } 3. What is temporal resolution and how is it produced in the electron
} } } } microscope?
} } } }
} } } } Thank you for your time. I greatly appreciate your efforts in helping
} me
} } } } understand more of this subject.
} } } }
} } } } Sincerely,
} } } } Jenny Wang
} } } }
} } } } -------------------------------------------------
} } } } Sent through Cyberbuzz- A Server for the Students
} } } } http://cyberbuzz.gatech.edu/
} } }
} } } --
} } } Thomas E. Phillips, Ph.D.
} } } Associate Professor of Biological Sciences
} } } Director, Molecular Cytology Core Facility
} } }
} } } 3 Tucker Hall
} } } Division of Biological Sciences
} } } University of Missouri
} } } Columbia, MO 65211-7400
} } } (573)-882-4712 (voice)
} } } (573)-882-0123 (fax)
} } }
} } }
} }
} } ______________________________________________________________________
} } Donald L. Lovett e-mail: lovett-at-tcnj.edu
} } Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} } P.O. Box 7718 fax: (609) 637-5118
} } The College of New Jersey
} } Ewing, NJ 08628-0718
}
} Geoff
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Feb 8 12:03:34 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 8 Feb 2002 09:57:29 -0800
Subject: Re: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John;
You are correct about the small CCDs of consumer digital cameras
have sever performance deficiencies due to their small pixel size. The F707
and other consumer digital cameras that use the same chip (2/3"-Nikon 5000
and Olympus E20N) suffer from noise even in visible light photographs. The
Nikon D1X and the new Canon EOS 1D(advertised 11.5 µm pixels) are
significant improvements, but cost $6000 just for the camera body! One
organization addressing this problem is
http://www.fredmiranda.com/Action_profilesPage/index.html where they discuss
and sell Photoshop plug-ins for noise reduction. Another digital camera site
I really like is:
http://www.imaging-resource.com/


John Mardinly
Intel


-----Original Message-----
} From: "DrJohnRuss%aol.com"-at-sparc5.microscopy.com
[mailto:"DrJohnRuss%aol.com"-at-sparc5.microscopy.com]
Sent: Thursday, February 07, 2002 4:50 PM
To: microscopy-at-sparc5.microscopy.com



In a message dated 2/7/02 12:22:59 PM,
tartenon-at-netscape.net-at-sparc5.microscopy.com writes:

} My Understanding is that you can increase that resolution using
Interpolation,
} but the real resolution of the image will be 3.2 Megapixels with the Nikon
} 995. I do not belive Olympus has 4 Megapixels non-interpolated (nor any
} available digital camera)

It's really a lot more complicated than that. The actual spatial resolution
of digital cameras is not simply the number of transistors on the chip (and
in that regard I have the new Sony DSC-f707 which has 5 megapixels on the
chip and produces awesome images, as compared to my Nikon 995). The problem
is that the single chip cameras use an array of filters to expose some
transistors to red, green and blue light. In order to get the image that is
stored, they use interpolation to fill in the color information where it was

not directly measured. The filters have broad wavelength coverage, and the
interpolation schemes are pretty good (lots of patents in that area). But by

direct measurement based on the Fourier power spectra none of these cameras
has a spatial resolution that approaches the value you would expect based on

the number of pixels in the stored image (which is usually the same as the
number of transistors, except for Fuji who save images that have even more
than that). For the Nikon and Sony cameras it is typical to find that the
actual resolution elements across an image - beyond which you have empty
magnification - number about 2/3 of the number of pixels. So a camera with
2000 transistors on each line (e.g., 2000 x 3000 = 6 megapixels) would
probably measure as having about 1300-1350 elements of resolution (in other
words, if you reduced the image to that size you would not really be
discarding any information, and any enlargement beyond that size is empty).

The spatial resolution of the 5 M-pixel Sony is not as good as a 35 mm film
camera, but it is extremely good all the same and certainly represents the
best quality available now (and probably good enough for a great many
applications) provided you store the image as uncompressed tif and don't
throw away the important details by allowing the camera to use jpeg
compression (which all of these cameras will do by default, to save memory).

The tonal resolution - range of brightness values from dark to bright - is
much more problematic. I have had good experience using high end consumer
digital cameras for bright field microsopy, but they don't begin to have
enough range for darkfield or fluorescence work. Remember that 8 bits is 256

grey levels, and even the cameras with internal 10 bits or more only produce

about 8 bits on output because of the conversion from a linear detector to a

film-like log output. Film easily covers several thousand discernible
brightness steps, which would require a 12 bit or more range. That's why
cooled cameras are used for these applications, and why the tiny chips used
in consumer cameras won't work (the small transistors simply can't hold
enough electrons to give that kind of dynamic range).

If you want to compare consumer type cameras, there is a wealth of unbiased
information available online at {http://www.dpreview.com/reviews/specs.asp} .

The discussion is centered on more typical photographic applications but
there are comparative images, and a lot of info.

Digital cameras are great, and they save a lot of time and money. But don't
expect an inexpensive consumer type camera to cover all applications.


From daemon Fri Feb 8 12:03:35 2002



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Fri, 8 Feb 2002 10:56:43 -0700
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Having read the comments on both sides I tend to think that part of the problem was due to quite a bit of
misunderstanding (mainly on our part). I too had the same negative reaction when I first read Ms. Wang's e-mail. The way
the first e-mail read I felt that the student was trying to have others solve her assignment problem ( which is why I
did not respond to her request). My reaction changed however when I read her latest e-mail explaining more the nature of
the exercise. I hope I learned from this experience and that in the future I will have a better attitude and ask first
for more information before I decide to give an answer.

Jordi

-----Original Message-----
} From: Oakley, Jeff [mailto:oakleyj-at-rayovac.com]
Sent: Friday, February 08, 2002 8:43 AM
To: Microscopy-at-sparc5.microscopy.com


I'm rather embarrassed that some of the members of this listserver were too
narrow minded and/or arrogant to see that Ms. Wang was using this group of
experts as a source, just as one would use a book. And besides, if she were
just going to "regurgitate" the information learned here, wouldn't she just
be doing the same with information pulled from a book?

I suddenly feel dirty somehow... Oh my God... It won't wash off!


Jeff (I'm in for it now) Oakley



} } I think your instructor's hope would be that you figured out the
} } answers to class problems on your own. Asking an expert in the field
} } and then simply regurgitating that information is a worthless
} } exercise. If you are going to invest the time and money required to
} } earn a degree, you might want to try to learn something along the
} } way. I am a big supporter of listservers but hate to see them used
} } in this way.
} }
} } }
} } } To whom it may concern:
} } }
} } } I am an undergraduate student enrolled at the Georgia Institute of
} } } Technology.
} } } I am in a biomedical engineering class, and we have a problem that we
must
} } } solve involving electron microscopes. I have a few questions that I
hope will
} } } get answered ASAP:
} } }
} } } 1. What are the advantages and disadvantages in using electrons for
} } } microscopy
} } } rather than light?
} } } 2. Does the wavelength of the electrons have anythign to do with the
spatial
} } } resolution that the microscope produces in the final picture?
} } } 3. What is temporal resolution and how is it produced in the electron
} } } microscope?
} } }
} } } Thank you for your time. I greatly appreciate your efforts in helping
me
} } } understand more of this subject.
} } }
} } } Sincerely,
} } } Jenny Wang



From daemon Fri Feb 8 12:26:16 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 08 Feb 2002 13:20:01 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Oakley, Jeff" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm rather embarrassed that some of the members of this listserver were too
} narrow minded and/or arrogant to see that Ms. Wang was using this group of
} experts as a source, just as one would use a book.

Thank you. Do all disagreements with your educational philosophy fall under this
umbrella?

} And besides, if she were
} just going to "regurgitate" the information learned here, wouldn't she just
} be doing the same with information pulled from a book?

The point is, she didn't use a book. Rather than look it up for herself she
tried to get someone else to give her the answers. Rather like the old rubric
about teaching a man to fish instead of giving him a fish.

} I suddenly feel dirty somehow... Oh my God... It won't wash off!
}
} Jeff (I'm in for it now) Oakley
}
} } } I think your instructor's hope would be that you figured out the
} } } answers to class problems on your own. Asking an expert in the field
} } } and then simply regurgitating that information is a worthless
} } } exercise. If you are going to invest the time and money required to
} } } earn a degree, you might want to try to learn something along the
} } } way. I am a big supporter of listservers but hate to see them used
} } } in this way.
} } }
} } } }
} } } } To whom it may concern:
} } } }
} } } } I am an undergraduate student enrolled at the Georgia Institute of
} } } } Technology.
} } } } I am in a biomedical engineering class, and we have a problem that we
} must
} } } } solve involving electron microscopes. I have a few questions that I
} hope will
} } } } get answered ASAP:
} } } }
} } } } 1. What are the advantages and disadvantages in using electrons for
} } } } microscopy
} } } } rather than light?
} } } } 2. Does the wavelength of the electrons have anythign to do with the
} spatial
} } } } resolution that the microscope produces in the final picture?
} } } } 3. What is temporal resolution and how is it produced in the electron
} } } } microscope?
} } } }
} } } } Thank you for your time. I greatly appreciate your efforts in helping
} me
} } } } understand more of this subject.
} } } }
} } } } Sincerely,
} } } } Jenny Wang

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Feb 8 12:52:03 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 08 Feb 2002 10:55:15 -0800
Subject: Answers to Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

You are correct, I am not an educator. I also agree with you whole
heartedly that when someone reads a text in search of information, they
learn a great deal more than they had planned on... It happens to me every
time I pick up a book in search of an answer to a question. I'm sure this
probably was the intent of the instructor.

The point I should have made in my previous post is that instead of shutting
someone down and "scolding" them (which is exactly what some of the listers
did) for what appeared to be a Cliff's Notes research method, the person
should have been guided to useful web pages or texts that would have made
them find the answers for themselves (which other posters did - kudos to
those).

It is possible to be helpful while at the same time not giving someone a
free ride.

I don't think my batteries are dead, Tom, I just think we are operating at
different voltages.

Jeff


-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Friday, February 08, 2002 9:26 AM
To: Oakley, Jeff
Cc: Microscopy-at-msa.microscopy.com


While I am no longer in academia, were that not
the case, I might have a different view. However,
consider the future relative to all students.

If they are aspiring to science, microscopy, etc.,
given their experience with MSA as a student,
what might their opinion be when they become
professionals? Isn't it possible that they could
have a bad taste in their mouth about MSA
in particular and the list specifically?

I don't belive that professionals should answer
student's questions in a concise and packaged
format. Rather, professionals should be used
primarily as pointers to sources of information.
Sometimes, they might provide specific data.
Either way, it should (emphasis) stimulate the
student towards their goal. If their motivation
for seeking information from professionals
is simply to get their assignment done, this
could lead to several consequences. One of
these is that they will not know the material
and will be unable to perform as an employee.

It seems that this point is what most listers
should be concerned about--and probably are.

Gary Gaugler, Ph.D.




From daemon Fri Feb 8 12:59:58 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 8 Feb 2002 12:54:46 -0600
Subject: RE: measurement and calibration onthe SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you,
Sure, I meant 2160 lines.

Vladimir

} -----Original Message-----
} From: Oldrich Benada [mailto:benada-at-biomed.cas.cz]
} Sent: Friday, February 08, 2002 2:20 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: measurement and calibration onthe SEM
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi,
} I think you have meant 2160 lines per mm giving 0.46 um between two
} adjacent lines (a standard replica grating); 2600 lines per mm should
} give .38 um spacing.
}
} Best regard from Prague
} Oldrich
}
}
}
} On 6 Feb 2002 at 15:30, Dusevich, Vladimir wrote:
}
} }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} ---------.
} }
} }
} }
} }
} } } -----Original Message-----
} } } From: Brian Wajdyk [mailto:electronmicroscopist-at-hotmail.com]
} } } Sent: Wednesday, February 06, 2002 10:47 AM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: measurement and calibration onthe SEM
} } }
} } }
} } } --------------------------------------------------------------
} } } ----------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } } of America
} } } To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } --------------------------------------------------------------
} } } ---------.
} } }
} } }
} } } Fellow Microscopists,
} } }
} } } SEM has historically been used for metrology of various
} } } structures. I can't
} } } seem to find much literature about the artifacts associated
} } } with this type
} } } of measurement. We do use the NIST standards to check the
} } } calibration of
} } } our equipment, but I haven't characterized how the different
} } } beam or sample
} } } parameters effect the measurements. What do you do? Has
} } } anyone figured out
} } } his or her actual accuracy and precision? We have found that
} } } we can safely
} } } give measurements within +/-5% taking into account most human
} } } and equipment
} } } errors. This is based on the precision of measurements
} made of NIST
} } } structures, measured the same way, over several years. On
} } } the other hand,
} } } the smallest structure we can measure on the standard is 2um
} } } (line and
} } } space. How do you determine at what magnification you
} will no longer
} }
} } Cheap grating replicas with 2600 lines per mm give 0.46 um per line,
} } and this is not too bad for calibrating 50,000 magnification. I am
} } happy I do not need certified standards.
} }
} } } guarantee the measurement? I see that my MRS-3 from Geller
} } } says it's for
} } } 10x to 50kX. How do they figure out that the max
} magnification it is
} } } useful? Maybe it as simple as being able to fit the
} structure on the
} }
} } Maximum useful magnification is very specimen dependant, especially
} } for low voltage and low vacuum modes. Of course, for digital images
} } it is possible to check brightness profiles and if they have slopes
} } on edges of features, then measure "size" on half height of
} the slope.
} } But I am not aware about publications which dependably
} justify this kind
} } of measurements (manipulations with brightness and contrast and
} } specimen tilt could change slopes significantly).
} }
} } } screen. If that were true, you would expect that the
} } } instrument would also
} } } be calibrated to a much higher magnification. How high could
} } } I say it is
} } } accurate to? Can I safely measure a 1000A line assuming no
} }
} } It depends on resolution for your microscope/specimens and on
} } calibration standard you are using. And I think periodic lines with
} } spacing 2 um not really good standard to measure feature with
} } the size of 0.1 um.
} }
} } } obvious issues
} } } (i.e. drift)? Can anyone educate me more on this topic or
} }
} } If you have visible drift during single exposure, then something
} } wrong with microscope or specimen preparation technique.
} }
} } } point me to
} } } resources?
} } }
} } }
} } } Things that could effect measurements (feel free to add to list):
} } } Drift (mechanical / beam)-
} }
} } exposure time should be small for significant drift.
} }
} } } Charging (obvious or stretching of image from a slow scan)
} } } Magnification (adjusted for each set of lens relays)
} }
} } Could be eliminated with proper calibration.
} }
} } } kV (surface vs. subsurface image)
} } } Working Distance
} }
} } Could be eliminated with proper calibration.
} }
} } } Delineation method (raised vs. depression, materials contrast)
} } } Amount of delineation (3D effect)
} } } Resolution (near resolution limit of SEM?)
} } } Contrast (or lack of, bright / dark line)
} } } Edge effect (bright line)
} } } Consistency between tools (calibration, etc.)
} } } Consistency between methods (SEM, TEM, ellipsometry, AFM, etc.)
} } } Operator's eye (where to measure. Measure outside to inside,
} } } center to
} } } center, out to out, in to in?)
} } } Variance in measured layer thickness (topography, sloped
} } } profile (i.e. base
} } } larger than top))
} } } Angle to beam
} } } Preparation methods (polish (i.e. smearing), cleave (i.e.
} } } pull of soft
} } } material), FIB (i.e. angle))
} } } Type of algorithm if doing it automatically (i.e. %50 threshold)
} }
} } Some of the things you have mentioned relate to specimen/experiment,
} } to stereology, but not to microscope. For example, if I
} need to measure
} } size of depression without sharp edges, I have to find (or
} at least to
} } declare)right procedure for it's measurements. May be I
} have to perform
} } stereo measurements and define an edge as a place, where a depth of
} } depression become equal to 0.1 um (or 10% of total depth, or
} } whatever else, depending on a study).
} }
} } And thank you for your extensive list - it is very helpful for
} } observation of the problem. And about additions to your list -
} } I think everybody can say something. For example recently I tried
} } to measure in ESEM thickness of a layer which, as it turned out,
} } was a viscous liquid...
} }
} } Regards,
} }
} } Vladimir
} }
} }
} } Vladimir M. Dusevich, Ph.D.
} } Electron Microscope Lab Manager
} } 3127 School of Dentistry
} } 650 E. 25th Street
} } Kansas City, MO 64108-2784
} }
} } Phone: (816) 235-2072
} } Fax: (816) 235-5524
} } Web: http://www.umkc.edu/dentistry/microscopy
} }
} }
} }
}
}
} +-----------------------------------+
} Oldrich Benada
} Acad. Sci. CR
} Institute of Microbiology
} Laboratory of electron microscopy
} Videnska 1083
} CZ - 142 20 Prague 4 - Krc
} Czech Republic
} +------------------------------------+
} Phone: +420-2-4752399
} Fax: +420-2-4752347
} WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm
}
}
}


From daemon Fri Feb 8 14:07:02 2002



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Fri, 08 Feb 2002 12:00:35 -0800
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No offence Jeff, but does anyone else think that this thread has taken on some
of the aspects of the Energizer Bunny?
;-)

"Oakley, Jeff" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Tom,
}
} You are correct, I am not an educator. I also agree with you whole
} heartedly that when someone reads a text in search of information, they
} learn a great deal more than they had planned on... It happens to me every
} time I pick up a book in search of an answer to a question. I'm sure this
} probably was the intent of the instructor.
}
} The point I should have made in my previous post is that instead of shutting
} someone down and "scolding" them (which is exactly what some of the listers
} did) for what appeared to be a Cliff's Notes research method, the person
} should have been guided to useful web pages or texts that would have made
} them find the answers for themselves (which other posters did - kudos to
} those).
}
} It is possible to be helpful while at the same time not giving someone a
} free ride.
}
} I don't think my batteries are dead, Tom, I just think we are operating at
} different voltages.
}
} Jeff
}
} -----Original Message-----
} } From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
} Sent: Friday, February 08, 2002 9:26 AM
} To: Oakley, Jeff
} Cc: Microscopy-at-msa.microscopy.com
} Subject: RE: Questions on the Electron Microscope
}
} Jeff: I am assuming from your rayovac.com you are not an educator.
} That doesn't disqualify your opinion but I would be more worried if
} other currently teaching academics widely expressed this view.
} Learning to look something up in the library is part of the teaching
} assignment. Finding the correct information and distilling it is not
} trivial. Most experts on the listserver could answer each of those
} questions in a few concise sentences. You would be hard pressed to
} find any source in a library in which you found the question followed
} by the answer. When you read the literature, lots of time you end
} up reading additional information on peripheral topics that add to
} the learning process. More importantly, students constantly give me
} sentences in their papers that are clearly paraphrased from the
} literature. I am not suggesting this constitutes plagiarism but it
} is often apparent from the sentence that they don't really understand
} what it means. They think they do but when I discuss it with them,
} they are unable to explain the sophisticated sentence in basic terms.
} Students frequently comment in my teaching evaluations that the most
} important thing they learned in class was that memorizing and
} rephrasing the literature doesn't equate to real understanding. If
} the instructor wanted them simply to ask an expert to get the bottom
} line answer, why didn't the instructor simply say it in lecture or
} give them a handout? Don't you think the instructor knew? Do you
} think a bioengineering class was designed to teach web surfing tools.
} I don't know if you should feel dirty but I think your batteries need
} recharging. Tom Phillips
}
} }
} }
} } I'm rather embarrassed that some of the members of this listserver were too
} } narrow minded and/or arrogant to see that Ms. Wang was using this group of
} } experts as a source, just as one would use a book. And besides, if she
} were
} } just going to "regurgitate" the information learned here, wouldn't she just
} } be doing the same with information pulled from a book?
} }
} } I suddenly feel dirty somehow... Oh my God... It won't wash off!
} }
} }
} } Jeff (I'm in for it now) Oakley
} }
} }
} }
} } } } I think your instructor's hope would be that you figured out the
} } } } answers to class problems on your own. Asking an expert in the field
} } } } and then simply regurgitating that information is a worthless
} } } } exercise. If you are going to invest the time and money required to
} } } } earn a degree, you might want to try to learn something along the
} } } } way. I am a big supporter of listservers but hate to see them used
} } } } in this way.
} } } }
} } } } }
} } } } } To whom it may concern:
} } } } }
} } } } } I am an undergraduate student enrolled at the Georgia Institute of
} } } } } Technology.
} } } } } I am in a biomedical engineering class, and we have a problem that we
} } must
} } } } } solve involving electron microscopes. I have a few questions that I
} } hope will
} } } } } get answered ASAP:
} } } } }
} } } } } 1. What are the advantages and disadvantages in using electrons for
} } } } } microscopy
} } } } } rather than light?
} } } } } 2. Does the wavelength of the electrons have anythign to do with the
} } spatial
} } } } } resolution that the microscope produces in the final picture?
} } } } } 3. What is temporal resolution and how is it produced in the
} electron
} } } } } microscope?
} } } } }
} } } } } Thank you for your time. I greatly appreciate your efforts in
} helping
} } me
} } } } } understand more of this subject.
} } } } }
} } } } } Sincerely,
} } } } } Jenny Wang
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)



From daemon Fri Feb 8 14:43:08 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Fri, 08 Feb 2002 15:33:21 +0100
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sending Jenny Wang's message to her Chairperson and UG Program Director was, in my eyes,
really unnecessary. Maybe it was just a nice try from Jenny Wang to get her homework done,
but this is really overdone.

Regards,

Stefan



Jim Quinn wrote:

} Don -
}
} College students should not broadcast messages seeking answers to elementary questions.
} Additionally, the use of "ASAP"
} was a bad idea.
}
} JQuinn
}
} PS: I sent Jenny Wang's message to her Chairperson and UG Program Director.

°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6210

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Fri Feb 8 14:49:00 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Fri, 08 Feb 2002 15:39:32 +0100
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sending Jenny Wang's message to her Chairperson and UG Program Director
was, in my eyes,
really unnecessary. Even if it was just a nice try from Jenny Wang to
get her homework done,
this is really overdone.

Regards,

Stefan



Jim Quinn wrote:

} Don -
}
} College students should not broadcast messages seeking answers to
elementary questions.
} Additionally, the use of "ASAP"
} was a bad idea.
}
} JQuinn
}
} PS: I sent Jenny Wang's message to her Chairperson and UG Program
Director.



°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6210

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Fri Feb 8 14:52:00 2002



From: anthony.borrelli-at-kodak.com
Date: Fri, 8 Feb 2002 15:45:59 -0500
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: Anthony R. Borrelli

Unsubscribe



From daemon Fri Feb 8 15:28:57 2002



From: Cavin Mooers :      cavinm-at-vsl.cua.edu
Date: Fri, 8 Feb 2002 16:05:06 -0500 (EST)
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My feeling is that those of you who lack a positve and constructive
response to a question that is posed, simply should not respond. I
have seen plenty of queries by "experts" which could be answered rather
simply by opening a book, but I certainly do not stoop to condescension.


Cavin Mooers, Research Assistant
Vitreous State Laboratory
The Catholic University of America
Hannan Hall
Washington, DC 20064
(202) 319-5346phone
(202) 319-4469fax





From daemon Fri Feb 8 15:38:07 2002



From: Ladd Research :      sales-at-laddresearch.com
Date: Fri, 08 Feb 2002 16:32:58 -0500
Subject: Re: help with buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shea,

I believe you may be looking Hanks' Balanced Salt Solution. We can get
it for you if you are looking to buy it, or contact me direct if you
just need some information on it.

Dr. Charles Duvic

--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
FAX: 1-802-660-8859
e-mail: sales-at-laddresearch.com

Quality Since 1955


Shea Miller wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Greetings all;
} can anyone provide the formulation for Hank's buffer? I have looked in all of my books (most, but not all of which, admittedly, lean
towards plants) and ca
}
} thanks in advance
} shea
}
} Dr. S. Shea Miller
} Agriculture & AgriFood Canada
} Eastern Cereal & Oilseed Research Centre
} Rm. 2068, K.W. Neatby Bldg.
} Central Experimental Farm
} Ottawa, Ontario,
} Canada K1A 0C6
} Phone: (613)759-1760
} Fax: (613)759-1701
} E-mail: millers-at-em.agr.ca


From daemon Fri Feb 8 15:47:27 2002



From: Doug Anderson :      danderson-at-schnabel-eng.com
Date: Fri, 8 Feb 2002 16:41:15 -0500
Subject: RE: vibration isolation standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List Members,

The killer in vibration impact on sensitive equipment is resonance. We
(Schnabel Engineering) have worked on many projects with varying equipment
vibration problems. The common denominator in all of them is that
resonances in the source, the vibration path (whether geological or
structural) and the receiver (the equipment and its mountings) combine to
produce an impact that must be ascertained. It is certainly desirable for
the impact to be determined in advance, because the range of mitigation
techniques is broader then. However, various retrofits are available. The
key is to use the appropriate retrofit.

Vibration, travelling in waves, is different than heat, and a solution of
just packing "stuff" around the site is generally inadequate; sometimes it
works, but that is then just dumb luck. I have used TEM equipment (in grad
school) and know that the column for a 100kV microscope has a substantially
different construction and configuration, and therefore substantially
different vibration response from a 1.2MV microscope. The taller tower of
the 1.2MV instrument will most probably have lower resonant frequencies than
the 100kV instrument. I am not sure if such information (mechanical
resonance) is available for them, but it certainly can be measured.

Mitigation techniques range from modification of the source, to barriers
(trenches or caissons around the facility), to floating floors, to dynamic
or passive absorbers. Each has its place, and the best (including cost!)
solution may be a combination of the above. Again, the reduction of
resonances is the key to successful vibration mitigation. I would be happy
to discuss such solutions with those interested.

As a side note, I first became acquainted with this list about a year and a
half ago, with respect to optical microscope standards. I am impressed with
the quality and quantity of contributions to the list.

Regards,

Doug Anderson

Douglas A. Anderson, PhD
Senior Consultant
Schnabel Engineering Associates (http://www.schnabel-eng.com)
510 East Gay Street
West Chester, PA 19380
Phone: 610 696-6066, Fax: 610 696-7771

} -----Original Message-----
} From: Lesley S. Bechtold [SMTP:lsb-at-jax.org]
} Sent: Wednesday, January 02, 2002 1:31 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Fwd: vibration isolation standards
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} } Happy New Year to everyone!
} }
} } We are setting up a totally new EM/LM lab - being built from the ground
} } up. We have told our engineers that we need to have a vibration-free
} } environment for optimum equipment operation. They would like to know
} } exactly what vibration is tolerable and what isn't. Are there any
} } standards or measurements out there that detail what limits can be
} } tolerated and what can't?
} }
} } Thank you!
} }
} } Lesley
} }
} } Lesley S. Bechtold
} } Supervisor, Biological Imaging
} } The Jackson Laboratory
} } 600 Main St.
} } Bar Harbor, ME 04609
} } 207-288-6191
}
} Lesley S. Bechtold
} Supervisor, Biological Imaging
} The Jackson Laboratory
} 600 Main St.
} Bar Harbor, ME 04609
} 207-288-6191
}
}
}
}
This e-mail including attached files is confidential. Its transmission is
solely as an accommodation for the benefit of the recipient. The recipient
bears the responsibility for checking its accuracy against corresponding
originally signed documents provided by Schnabel Engineering Associates,
Inc. If you received this e-mail in error, its use is prohibited. Please
destroy it and immediately notify postmaster-at-schnabel-eng.com



From daemon Fri Feb 8 17:05:47 2002



From: Xianglin Li :      Xianglin_Li-at-student.uml.edu
Date: Fri, 08 Feb 2002 17:57:09 -0500
Subject: Do you have any recommended books?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all;

I have two sets of data to interpreted:
1) X-ray rocking curve data, to analyze the defect (damage) of the
wafer surface.
2) XPS data, to trace how the wafer surface composition will change
with the increasing of sputtering time.

I need to have some fundamental idea of these two theories to
interpreted data. Do you have any recommendations of the textbooks, or
other papers I should read?

Any kind of help will be appreciated!

Xianglin Li

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Fri Feb 8 17:23:39 2002



From: Beauregard :      beaurega-at-westol.com
Date: Fri, 08 Feb 2002 18:46:03 -0500
Subject: Re: Polaroid Sprintscan 45 Ultra film holders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers:

Looks like there are different opinions out there. Before this turns into a
flame war, I would personally like to know what the general opinion is. I
therefore propose a poll. Just send me an email with nothing but one of the
following numbers in the subject line, and I will tally those votes and
provide a summary in a week (if I get enough emails):

1) Students should work through their assignments alone. Let's not support
laziness at all.
2) We should help them help themselves by pointing them to general
microscopy books.
3) We should take their questions and point them to specific microscopy
books dealing with their problems
4) We should encourage them to contact us offline so we can test their
sincerity and then either help them or not. This should be posted on the
server to avoid redundancy.
5) We should help them with their questions by trying to answer them in a
general way
6) We should go all out and answer their questions as best as we can.

I hope the statistics will not be skewed by a zillion students who send me
"6" as an answer ;-)

Any suggestions are welcome! I also promise not to misuse the email
addresses!

} } } please send the email to mb-at-soft-imaging.com { { { { { {

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Oakley, Jeff [mailto:oakleyj-at-rayovac.com]
Sent: Friday, February 08, 2002 11:46 AM
To: Microscopy-at-sparc5.microscopy.com


Tom,

You are correct, I am not an educator. I also agree with you whole
heartedly that when someone reads a text in search of information, they
learn a great deal more than they had planned on... It happens to me every
time I pick up a book in search of an answer to a question. I'm sure this
probably was the intent of the instructor.

The point I should have made in my previous post is that instead of shutting
someone down and "scolding" them (which is exactly what some of the listers
did) for what appeared to be a Cliff's Notes research method, the person
should have been guided to useful web pages or texts that would have made
them find the answers for themselves (which other posters did - kudos to
those).

It is possible to be helpful while at the same time not giving someone a
free ride.

I don't think my batteries are dead, Tom, I just think we are operating at
different voltages.

Jeff


-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Friday, February 08, 2002 9:26 AM
To: Oakley, Jeff
Cc: Microscopy-at-msa.microscopy.com


At 10:28 PM 02/07/02 -0500, Beauregard wrote:
} Polaroid did make a few of them. I saw a metal one, photocopied it
} and measured it. I have the insert spec's but not at home here. I
} could scan it and post it on my web page.

Hi Don,

I have scanned in the photocopy of the special insert for the 4X5 holder.
I made a mistake about it being recessed. The SS45 comes with one (2¼x2¼?)
holder that is recessed and that was what I recalled at home as being
recessed. After looking at the photocopy of an official insert, I realized
my mistake.

The holder is nothing but a totally flat piece of steel with 6 pins
sticking up, a rectangular hole in it to allow transmitted light to shine
through the negative, and along the one outside edge of the insert are two
slots that fit into the two pins on the 4x5 holder.

I will post two JPG images of the insert(s) at:

http://www.westol.com/~beaurega/ss45.htm

I included a scanned image of a steel insert / holder that came standard
with the scanner. Notice the two holders have identical slot alignment in
my one image. Adjust the DPI of the image to get your laserjet printed
insert image to line up with the two pins in the 4x5 holder. Then use this
accurate template to make or have made the insert.

One could use a double layer of old film to make the equivalents of the pin
posts to keep the film in register.
The whole thing is painted black. Use a Dremel tool cut off wheel to make
the slots.

Hope this helps.

Paul Beauregard




From daemon Fri Feb 8 18:19:33 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 8 Feb 2002 18:12:07 -0600
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree, Stefan. Not only overdone, but ugly. Since when did we become proctors of other people's courses? I was satisfied with Jenny's answer. If she had ever considered a career in electron microscopy, I'll bet she's reconsidering now.

Randy Tindall
EM Core
University of Missouri

-----Original Message-----
} From: Stefan Geimer
To: MSA Listserver
Sent: 2/8/2002 8:39 AM


Sending Jenny Wang's message to her Chairperson and UG Program Director
was, in my eyes,
really unnecessary. Even if it was just a nice try from Jenny Wang to
get her homework done,
this is really overdone.

Regards,

Stefan



Jim Quinn wrote:

} Don -
}
} College students should not broadcast messages seeking answers to
elementary questions.
} Additionally, the use of "ASAP"
} was a bad idea.
}
} JQuinn
}
} PS: I sent Jenny Wang's message to her Chairperson and UG Program
Director.



°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6210

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Fri Feb 8 19:42:14 2002



From: Helvey, Marc :      Marc.Helvey-at-vlsistd.com
Date: Fri, 8 Feb 2002 17:33:35 -0800
Subject: calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Mark -

VLSI Standards is in the midst of releasing (the product is in beta at this
juncture) a NIST Traceable, 100 nm pitch standard for use with SEMs. The
accuracy is equal to or less than 1 nm in most cases. Besides 100 nm, it
will also be certified for 4 other pitch values. It will come in various
wafer / die form factors to be able to accommodate CD-SEMs utilizing
automated handlers, as found in the Semiconductor and related industries.

Please contact me directly offline and I'd be glad to provide you
information on this exciting new product.

Regards -

Marc Helvey
Strategic Accounts Manager
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
Phone: (408) 428-1800, ext. 108
E-Mail: marc.helvey-at-vlsistd.com {mailto:marc.helvey-at-vlsistd.com}
Internet: http://www.vlsistandards.com




-----Original Message-----
} From: Windland, Mark J (MN14) [mailto:Mark.Windland-at-honeywell.com]
Sent: Friday, February 08, 2002 7:51 AM
To: Microscopy-at-sparc5.microscopy.com


We need to purchase a new calibration standard for our SEM that gets us down
to the next level. We need to have accuracy down to 0.010 microns. We have
been using the Geller MRS-3 and now are considering purchasing the MRS-4
traceable standard.
I believe this will give us what we need but I was wondering if there are
other standards out there that are better, the same, worse? I need to hear
from the calibration specialists out there.
Thanks,

Mark Windland
Honeywell
Minneapolis, Minnesota
763-954-2845


From daemon Fri Feb 8 21:12:09 2002



From: Dr Deborah Stenzel :      d.stenzel-at-qut.edu.au
Date: Fri, 8 Feb 2002 22:57:06 -0600
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I don't think it is a simple answer to your questions. If the request is
like
the subject of these discussions, then I would say number three. If the
student has researched the basic information (showing an earnest effort
at learning), and asks for help with understanding what they have found,
or carrying it further, then I see no problem with the experts discussing
and feeding information to the student. Giving them "food for thought"
should not be a problem.

Darrell

Mike Bode {mb-at-Soft-Imaging.com} on 02/08/2002 06:19:42 PM

To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
cc:


Hello Listers:

Looks like there are different opinions out there. Before this turns into a
flame war, I would personally like to know what the general opinion is. I
therefore propose a poll. Just send me an email with nothing but one of the
following numbers in the subject line, and I will tally those votes and
provide a summary in a week (if I get enough emails):

1) Students should work through their assignments alone. Let's not support
laziness at all.
2) We should help them help themselves by pointing them to general
microscopy books.
3) We should take their questions and point them to specific microscopy
books dealing with their problems
4) We should encourage them to contact us offline so we can test their
sincerity and then either help them or not. This should be posted on the
server to avoid redundancy.
5) We should help them with their questions by trying to answer them in a
general way
6) We should go all out and answer their questions as best as we can.

I hope the statistics will not be skewed by a zillion students who send me
"6" as an answer ;-)

Any suggestions are welcome! I also promise not to misuse the email
addresses!

} } } please send the email to mb-at-soft-imaging.com { { { { { {

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Oakley, Jeff [mailto:oakleyj-at-rayovac.com]
Sent: Friday, February 08, 2002 11:46 AM
To: Microscopy-at-sparc5.microscopy.com


Tom,

You are correct, I am not an educator. I also agree with you whole
heartedly that when someone reads a text in search of information, they
learn a great deal more than they had planned on... It happens to me every
time I pick up a book in search of an answer to a question. I'm sure this
probably was the intent of the instructor.

The point I should have made in my previous post is that instead of
shutting
someone down and "scolding" them (which is exactly what some of the listers
did) for what appeared to be a Cliff's Notes research method, the person
should have been guided to useful web pages or texts that would have made
them find the answers for themselves (which other posters did - kudos to
those).

It is possible to be helpful while at the same time not giving someone a
free ride.

I don't think my batteries are dead, Tom, I just think we are operating at
different voltages.

Jeff


-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Friday, February 08, 2002 9:26 AM
To: Oakley, Jeff
Cc: Microscopy-at-msa.microscopy.com


Dear all

I've been reading the discussions on this topic with interest, as a
lecturer who sets similar EM assignment questions for undergraduate
students.

Last year, a couple of my students posted their assignment questions
to the listserver. After an initial feeling of annoyance that the
students were being lazy, and not seeking out information for
themselves, I eventually decided that this probably wasn't such a bad
thing - as Mike said in his email, we are supposed to be teaching
students to identify and utilize different sources of information!

In my case, I hadn't directly told the class about the listserver, so
my students had either sought it out by themselves, or had actually
read through a list of suggested reference texts and websites which
had been given to them early in the semester. Either way, this was
something to be encouraged!

I certainly don't think the listserver is the place where we should
provide full and detailed answers to students' assignment questions -
and particularly not when demanded "ASAP", and without evidence of
the student having done any of their own homework on the topic!

However, I also share the views expressed in some earlier messages
that we are a useful "resource" for students. For those who have the
time and inclination to respond to students' requests, I support the
approach of offering some basic information (brief, easy to
understand) as a starting point, and then suggesting that the student
refer to texts, papers or other sources. Indeed, this is exactly
what happened with my students, and both presented assignments with
information gleaned from a wide variety of sources. Many thanks to
those of you who responded in this way.


Cheers
Deb
*****************************************************
Dr Deborah Stenzel
Lecturer (Microbiology)
School of Life Sciences
and
Applications Specialist (Biological)
Analytical Electron Microscopy Facility
Queensland University of Technology
GPO Box 2434
Brisbane 4001
Australia

Phone + 61 7 3864 5036
Fax + 61 7 3864 5100
email d.stenzel-at-qut.edu.au

http://www.sci.qut.edu.au/aemf


From daemon Fri Feb 8 23:09:18 2002



From: Peter Ingram :      p.ingram-at-cellbio.duke.edu
Date: Fri, 8 Feb 2002 22:26:07 -0600
Subject: LKB Ultratome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a perfectly good LKB Ultramicrotome ("Ultratome")
Model #2088 (circa. 1976) for which we have no need. We have several
others and we DO need the space! It also has most of the parts for a
cryokit.

If anyone is interested: its yours for FREE! Just come and
pick it up or arrange for shipment.

Please feel free to call or leave a message at any time.
--
Peter Ingram
Sr. Physicist, RTI
Adj. Professor of Pathology,
Duke University Medical Center
Box 90319
LaSalle Street Extension
DURHAM NC USA 27708-0319

Tel: (919) 660-2695
Fax: (919) 660-2671
e-mail: p.ingram-at-cellbio.duke.edu
http://152.3.167.174/AEM_LAB.html


From daemon Sat Feb 9 01:39:40 2002



From: pjp6 :      pjp6-at-dana.ucc.nau.edu
Date: Sat, 09 Feb 2002 00:33:41 -0700
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In much less time than it took to find addis for Jenny Wang's chairperson and
program director he could have referred Jenny to Bozzola/Russell or any other
EM text for the answers. This string has become more about those who want to
educate and help vs. those who would judge and punish.

Still listening and learning.
Pete Polsgrove



} ===== Original Message From Stefan Geimer {stefan.geimer-at-yale.edu} =====
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Pete Polsgrove
NAU Flagstaff, AZ.
pjp6-at-dana.ucc.nau.edu
micro2001p-at-netscape.net



From daemon Sat Feb 9 05:28:22 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Sat, 9 Feb 2002 11:23:33 -0000
Subject: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been stunned by how many would deny answers to a student. I
have to confess that I did not analyse the enquirer's motives, and
offered some simple answers to her questions

Is the objection that the enquiry came via the internet, so was
directed to all on the list?
Would your reaction have been the same if the student made a more
personal, targeted approach
a) Called at your lab/office to ask questions
b) Wrote asking questions
c) Phoned/Faxed you asking questions

I think we have to acknowledge that the pre-internet world of the
printed page and the post-internet
world are totally different. Students are under immense pressure, not
least under the burgeoning weight of the paper literature, and simply
cannot afford the time to plough through roomfuls of books, however
good this would be for their souls. They need entry points to a
problem, and they should be congratulated for using all of the
facilities currently at their disposal to get there. If that changes
the way educators have to go about assessment of project work so be
it. That is our professional problem, not the student's problem. This
list has a significant educational role at all levels within research
and tertiary education, and I would be saddened if barriers are
erected against enquiries from students.


Dr. Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility

Inveresk Cottage
26, Carberry Road
Inveresk
Musselburgh
Midlothian
EH21 8PR
Tel: +44 131 665 6062
FAX +44 131 653 6248
Mobile 07710 585 401
Dr. Chris Jeffree
Inveresk Cottage
26, Carberry Road
Inveresk
Musselburgh
Midlothian
EH21 8PR
Tel: +44 131 665 6062
FAX +44 131 653 6248
Mobile 07710 585 401



From daemon Sat Feb 9 05:40:39 2002



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 9 Feb 2002 03:32:26 -0800 (PST)
Subject: Re: Answers to Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gary and All,

As for "bad taste" from the MSA response, I taste no such displeasure.

I must say the questions asked were vague and rather general. Have I
missed something? In all this communication, have we heard from the course
professor explaining, defending, or otherwise making a statement as to
what he or she was requiring of the student?! Why is not the professor
explaining this material to the student? The professor should be the
FIRST resource, or at least provide the student with a rudimentary
understanding of the topic and perhaps assigning readings from handouts or
materials on reserve in the library.

I teach a dedicated biomicroscopy course (optical light microscopy, TEM
amd SEM) to upper division biology students, and have for 9 years. I
would never just suggest students be turned out to fend on their own. I
provide a a number of reserve textbooks and a huge number of handouts. I
ALWAYS suggest if students are having a difficult time finding answers to
questions I have proposed, they come to me FIRST!! In this manner, I
have control over the ratio of student ability and information available.

I believe the hallmark of an educator is to entice and DIRECT the student
in a manner of investigation, not to just through out a bunch of
questions, allowing the student to randomly be come up with the answers,
some of which depending of the source may be incorrect.

I must admit, when I saw the original email, my thoughts were divided into
two direction: 1) here is a student who is looking for quick answers to
some vague, rather general questions; and 2) here is a professor who is
too busy with something else and has not put forth the foundation from
which the student could asked specific questions about the general topics,
e.g., "I have read this about the subject and do not understand. Is there
someone on the listserver who can explain it differently from how I
interpret the subject?"

Again, to some extent this is the responsibility of the professor.

Enough said...

Ken
--
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203





From daemon Sat Feb 9 05:58:55 2002



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 9 Feb 2002 03:50:02 -0800 (PST)
Subject: Re: help with buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shea,

According to 'Staining Procedures', Edited by George Clark, 4th
edition, Williams and Wilkins, 1981, page 22:

Hanks' Balanced Salt Solution (Hanks' BSS)

1) CaCl2- 2H2O 185.5 mg/liter
2) KCl 400.0 mg/liter
3) KH2PO4 60.0 mg/liter
4) MgSO4-7H2O 200.0 mg/liter
5) NaCl 8000.0 mg/liter
6) NaHCO3 350.0 mg/liter
7) Na2HPO4 47.5 mg/liter
8) Dextrose 1000.0 mg/liter
9) Phenol Red, Na 17.0 mg/liter

Good luck!
Ken


--
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203





From daemon Sat Feb 9 10:35:42 2002



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu
Date: Sat, 9 Feb 2002 10:27:25 -0600
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ===== Original Message From Dr Deborah Stenzel {d.stenzel-at-qut.edu.au} =====
Hello Group,
I agree with Deb's post. Following this thread, I got to thinking that it
is maybe time WE (as educator/citizens) do a little reading. Times they are a
changin'. Try to find this article: Get Ready for the Net Generation, by Mark
L. Alch, taken from the February 2000 issue of Training & Development. I
found it in the Human Resources 01/02 Annual Editions, ISBN 0-07-243342-6.
The way the new generations are learning happens to be a little different than
the way most of us did. We not only have to understand how they learn, but we
will also have to understand what motivates them once we hire them.
Randy



} Dear all
} Last year, a couple of my students posted their assignment questions
} to the listserver. After an initial feeling of annoyance that the
} students were being lazy, and not seeking out information for
} themselves, I eventually decided that this probably wasn't such a bad
} thing - as Mike said in his email, we are supposed to be teaching
} students to identify and utilize different sources of information!
}
} In my case, I hadn't directly told the class about the listserver, so
} my students had either sought it out by themselves, or had actually
} read through a list of suggested reference texts and websites which
} had been given to them early in the semester. Either way, this was
} something to be encouraged!
}
} I certainly don't think the listserver is the place where we should
} provide full and detailed answers to students' assignment questions -
} and particularly not when demanded "ASAP", and without evidence of
} the student having done any of their own homework on the topic!
}
} However, I also share the views expressed in some earlier messages
} that we are a useful "resource" for students. For those who have the
} time and inclination to respond to students' requests, I support the
} approach of offering some basic information (brief, easy to
} understand) as a starting point, and then suggesting that the student
} refer to texts, papers or other sources. Indeed, this is exactly
} what happened with my students, and both presented assignments with
} information gleaned from a wide variety of sources. Many thanks to
} those of you who responded in this way.
}
}
} Cheers
} Deb
} *****************************************************
} Dr Deborah Stenzel
} Lecturer (Microbiology)
} School of Life Sciences
} and
} Applications Specialist (Biological)
} Analytical Electron Microscopy Facility
} Queensland University of Technology
} GPO Box 2434
} Brisbane 4001
} Australia
}
} Phone + 61 7 3864 5036
} Fax + 61 7 3864 5100
} email d.stenzel-at-qut.edu.au
}
} http://www.sci.qut.edu.au/aemf



From daemon Sat Feb 9 12:58:26 2002



From: Richard Cole :      rcole-at-wadsworth.org
Date: Sat, 9 Feb 2002 13:51:23 -0500
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear All

I can not believe all the unnecessary and self-righteous email one student
has generated by asking a few questions! For those of you out there who
feel it was "wrong" for a student to ask these questions, don't answer them.
But as for emailing her Dept. head, when did this list become some sort of
regulated police like forum? If this is what it has degraded to, I what
off! As I tell my children and student alike there are no stupid or bad
questions. As professional (at least I thought we all were before this) I
believe that it is not only out duty but our obligation to help other learn.
I don't mean doing there homework for them, but the response that this
girl/women received embarrassed and ashamed me. I simple reply guiding her
were to search for the answers could have saved everyone a lot of time and
seem energy as well. As for people in general asking questions that the
answers can be found in books, again if you don't want to answer them,
don't. As for me, I am not the smartest man in the world nor do not know
every thing and naively thought that this is what forum/list servers like
this one were for. Guess I was wrong

Richard Cole
Research Scientist III
Director of the Laser Microsurgery and Advanced Light Microscopy Core Unit
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-486-4901 Fax



From daemon Sat Feb 9 14:13:29 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Sat, 9 Feb 2002 10:06:34 -1000 (HST)
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I looked into the background of the student's course early on and sent a
brief explanation to the List, twice, but it never arrived, as far as I
can tell. I'll tack in on below. One of the points of this biomedical
engineering course that has been overlooked is that it is a PBL course.

PBL is a new philosophy of teaching that is being used, in part, by
various medical schools and othe programs. I don't know a whole lot about
it, but I know it exists because the University of Hawaii was the first
place to embrace PBL wholly, instead of partially as at other
institutions. Basically, the medical students here have no formal courses,
but are thrown directly out into the clinics from day one, and are
encouraged to learn what they need to know to do their "jobs" fairly
independently. I'm sure this is an oversimplification, but not by a
lot. There was a hue and cry from many of the instructors - how do you
learn gross anatomy without a gross anatomy class and a cadaver? But I
guess they worked it out. When I ask the current crop of students if they
like it, they say they do. There's more of an emphasis on learning by
doing and asking than just sitting in classrooms all day and with books
all night.

However, here's the point - they are encouraged to go out and learn how to
find answers in the world, using all resources from the library to asking
experts to the Internet. And they are told not to just ask the
teacher".

Do I agree with this method? Mostly no, probably because I didn't learn
that way, and I'm old enough to be kinda set in my ways. . Is it
working? Apparently yes. Now that I'm not an official student, is that
the way I learn *now*? Yes, it is!

This is not an endorsement of the PBL system (y'all need to do some
research on it to understand how the philosophy, as do I), but merely an
explanation of why the students are asking the questions. And then ignore
or guide them, whatever you wish.

Aloha,
Tina


Message that did not reach the List:

I have received several emails recently about TEMs, as have several of you
as well as this List. At first I dismissed tham as being at about the same
level as Mrs. Jones' 6th grade science class who each individually emailed
me to ask "How does an electron microscope work?" However, I looked into
this and found out that this recent spate are from a Biomedical
Engineering class at Georgia Tech. There are 60 students, split up into
teams, involved in a Problem Based Learning curriculum, which encourages
using all resources available, from the library to interviewing
experts. Their project is an interesting one - although I deleted the
original questions, I think it involves designing an original, viable
improvement for the electron microscope, especially in areas that would
allow them to create a 4D database of living cells and their cellular
functions.

I received a couple of messages from students who had apparently done
their library research and were able to ask thoughtful, reasoned
questions. I feel that the ones who simply regurgitate the instructors'
list of questions *do* need to do more background research and then
formulate specific questions and direct them to the particular experts
in the field. But they have only a couple of weeks on this assignment, so
I guess I understand their "spamming" the List to find those experts!

I thought giving you all the background on the project would help you
decide how to respond to the messages. It is an interesting mental
exercise to think about how to build such an electron microscope. Perhaps
they will!


****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************






From daemon Sun Feb 10 11:39:53 2002



From: flcy-at-att.net
Date: Sun, 10 Feb 2002 17:21:38 +0000
Subject: Re: help with buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Sun Feb 10 20:31:57 2002



From: max.sidorov-at-amd.com
Date: Sun, 10 Feb 2002 18:22:04 -0800
Subject: TEM: ctfExplorer update (v. 0.999a)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:

FYI: ctfExplorer has been updated and it is still free for all.

New features/improvements/fixes:

- Corrected an error in the formula used for Focal Spread calculation which caused too strong damping by temporal envelope at high frequencies. Thanks to Michael O'Keefe, Peter Tiemeijer and Uwe Lucken for pinpointing this error.

- Added a posibility to change values for high voltage and objective lens current instabilities (along with chromatic aberration and energy spread they affect the value of focal spread)

- Added a possibility of editing/saving/restoring of the microscope list

To the best of my knowledge, the software does not have any nasty bugs. It
is tested under Windows 95/98/NT4/2000.

Please give it a try. Please DO direct your suggestions and comments to sidorov-at-yahoo.com
I hope you'll find the software useful.

Here is the link: http://clik.to/ctfexplorer (always use this link. it will
direct you to the right location). Direct link: http://www.maxsidorov.com/ctfexplorer

Enjoy,
__________________________________
Max Sidorov, Ph.D.
max.sidorov-at-amd.com


----------Additional Info----------
ctfExplorer is a highly interactive program which calculates/displays the contrast
transfer function of TEMs. I know that there are similar programs floating
around but ctfexplorer does not only 1d but also 2d calculations/display
with 2-fold and 3-fold astigmatism imposed. There are other unique features
to it. All parameters (defocus, voltage, Cs, etc) can be changed
interactively.

Features
- Calculates 1-Dimensional CTF
- Calculates 2-Dimensional CTF
- Calculates Defocus Map
- Calculates point-to-point resolution, Lichte defocus and info limit
- Shows the effects of 2-Fold and 3-Fold astigmatism
- Allows to change Defocus, 2-Fold and 3-Fold astigmatism in real time
- Shows what happens to 1D CTF in different directions when there's astigmatism
- Displays the damping envelopes
- Allows to select a microscope from a list of microscopes
- Allows to create a custom microscope
- Allows to change HT, Cs, Cc, Energy Spread, Convergence for any microscope
- 2 modes of operation: CTF Explorer (to see everything) and CTF Plotter
- Compares 2 microscopes or 2 settings for 1 microscope
- Saves 1D CTF, 2D CTF and "Defocus Map" as bitmaps or metafiles
- Exports 1D plots to tab-delimited text format





From daemon Sun Feb 10 22:01:41 2002



From: hazrat.hussain-at-iw.uni-halle.de ()
Date: Sun, 10 Feb 2002 21:55:57 -0600
Subject: Ask-A-Microscopist:TEM block copolymer

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (hazrat.hussain-at-iw.uni-halle) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
February 10, 2002 at 11:35:50
---------------------------------------------------------------------------

Email: hazrat.hussain-at-iw.uni-halle
Name: Hussain

Organization: University of Halle

Education: Graduate College

Location: Germany

Question: Dear Sir,
I have stained a block copolymer isothermally crystallized samples
with RuO4 vapors. The block copolymers constitute poly(ethylene
oxide)(semicrystalline block) and poly(perfluorohexylethyl
methacrylate) as second block.
The structure is as
PFMA-b-PEO-b-PFMA
PEO block length is 227 EO units(20000 g/mol) and each PFMA block
was 5-7 PFMA units.
The TEM morphology that we got from this sample was showing a
layered or lamellar structure.There are dark lines and bright lines.
Now I dont know, which block has been stained with RuO4.
The second question is again the staining assigning problem, I got
TEM pictures from solutions of the same block copolymers, the samples
were stained again with the same dye. we got some spherical micelles
in the TEM picture, but we dont know, the staining blocks.
I hope u will consider answer my questions. I will be grateful
with my best regards
Hussain

---------------------------------------------------------------------------


From daemon Mon Feb 11 06:19:44 2002



From: Dr Adam Papworth :      ajp5-at-liverpool.ac.uk
Date: Mon, 11 Feb 2002 12:08:15 +0000
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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--
Dr Adam Papworth,
Senior Experimental Officer,
Department of Engineering,
The University of Liverpool,
Liverpool,
L69 3GH, UK.

Phone
(Work) 0151 794 4672
(Mobile) 0151 794 7587
07970 24 7587
(Home) 0151 283 8596
(FAX) 0151 794 4675
e-mail ajp5-at-liv.ac.uk




From daemon Mon Feb 11 06:55:12 2002



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Mon, 11 Feb 2002 08:48:27 -0400
Subject: Re: Answers to Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gee, maybe this whole thread is just a Sociology experiment to gauge
the response of a small group of specialists to a contentious issue! For
my $0.02 ($0.033 Canadian), pick up a copy of "Silicon Snake Oil" by
Cliff Stoll. Relevant reading, I think.

Cheers,

Jim

--
James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Mon Feb 11 07:50:20 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 11 Feb 2002 08:40:08 -0500
Subject: staining of block copolymers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hussain Hazrat wrote:
============================================
Question: Dear Sir,
I have stained a block copolymer isothermally crystallized samples
with RuO4 vapors. The block copolymers constitute poly(ethylene
oxide)(semicrystalline block) and poly(perfluorohexylethyl
methacrylate) as second block.
The structure is as
PFMA-b-PEO-b-PFMA
PEO block length is 227 EO units(20000 g/mol) and each PFMA block
was 5-7 PFMA units.
The TEM morphology that we got from this sample was showing a
layered or lamellar structure.There are dark lines and bright lines.
Now I dont know, which block has been stained with RuO4.
The second question is again the staining assigning problem, I got
TEM pictures from solutions of the same block copolymers, the samples
were stained again with the same dye. we got some spherical micelles
in the TEM picture, but we dont know, the staining blocks.
I hope u will consider answer my questions. I will be grateful
with my best regards
Hussain
========================================================
This kind of question is extremely difficult to "call" on the basis of first
principles (or logic). You really need to see one or two "knowns", and then
you can see which way the area percent of the dark vs. white changes.

If you are seeing some "spherical" features from solution precipitation, and
it is just a guess, it might be that you are seeing segregation of
homopolymer into a more traditional kind of morphology for these kinds of
systems. At least when we have seen such features in other block copolymer
systems that was our conclusion.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Mon Feb 11 07:50:20 2002



From: Jon Ekman :      ekman-at-bio.fsu.edu
Date: Mon, 11 Feb 2002 08:45:19 -0500
Subject: EM: Exploding Kevex LN2 level sensor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Just wanted to give a heads up for anyone with older Kevex LN2 cooled
EDS systems. On Friday, our Kevex LN2 level sensor just exploded after
15years of service. Normally we remove the sensor (unplugged from the
system) and place it horizontal in a special holder while we fill the
LN2 then we dry it off and put it back in place. Today the metal top
blew off and hit me in the leg. No injuries except the loud bang may
have shaved a year or so off my life. Luckly, we had a second sensor
on hand for replacement.

If any one has a good explanation why the metal cover decided to tear
itself away from the Styrofoam insulation after all these years we would
like to hear from
you.

TIA

Jon Ekman
Florida State University
Biological Science Imaging Resource
119 Bio Unit I, 4370
Tallahassee, FL 32306
tel: 850.644.6519
fax: 850.644.0481



From daemon Mon Feb 11 07:51:48 2002



From: Petr Schauer :      Petr-at-isibrno.cz
Date: Mon, 11 Feb 2002 14:46:09 +0100
Subject: Microscopy Laboratories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

Let me inform you the list of microscopy laboratories at the "Petr's
Microscopy Resources" has been completely rebuilt. You can check it
at the
http://www.petr.isibrno.cz/microscopy/laboratories.php .

Furthermore, the form for a new link submission has been revised to a
great extent. Therefore, the addition of a new link to your
laboratory is very easy and safe now, and your submission will be
very appreciated. You can find the submission form at the new location
http://www.petr.isibrno.cz/microscopy/PMRform.php .

Regards,

Petr Schauer
+---------------------------------------------------------------------+
| Dr. Petr Schauer tel.: (+420 5) 41514313 |
| Head of Electron Microscopy Laboratory fax : (+420 5) 41514404 |
| INSTITUTE OF SCIENTIFIC INSTRUMENTS (+420 5) 41514402 |
| ACADEMY OF SCIENCES OF THE CZECH REPUBLIC e-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno petr-at-dp.fce.vutbr.cz |
| Czech Republic www: http://www.petr.isibrno.cz/ |
+---------------------------------------------------------------------+


From daemon Mon Feb 11 07:57:10 2002



From: Michael Herron :      herro001-at-umn.edu
Date: Mon, 11 Feb 2002 07:51:11 -0600
Subject: Re: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
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All,

OK so it is a given that consumer grade cameras have relativly poor low
light performance. That said, are there cameras that have better than
average lowlight performance? Are any of the consumer cameras capable
of binning?

Mike


"Mardinly, John" wrote:
}
}
} John;
} You are correct about the small CCDs of consumer digital cameras
} have sever performance deficiencies due to their small pixel size. The F707
} and other consumer digital cameras that use the same chip (2/3"-Nikon 5000
} and Olympus E20N) suffer from noise even in visible light photographs. The
} Nikon D1X and the new Canon EOS 1D(advertised 11.5 µm pixels) are
} significant improvements, but cost $6000 just for the camera body! One
} organization addressing this problem is
} http://www.fredmiranda.com/Action_profilesPage/index.html where they discuss
} and sell Photoshop plug-ins for noise reduction. Another digital camera site
} I really like is:
} http://www.imaging-resource.com/
}

--

________________________________________________________
/ Michael J. Herron, U of MN, Dept. of Pediatrics/BMT /
/ herro001-at-umn.edu /
/ 612-626-4321 Mpls MN 55455 /
/_______________________________________________________/


From daemon Mon Feb 11 08:00:06 2002



From: Paul.Nolan-at-alcan.com
Date: Mon, 11 Feb 2002 08:54:12 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I see we have the equivalent of a prison snitch in our midst ..or more
appropriately in this case ..a school yard tattle-tale
Jim Quinn wrote:

} Don -
}
} College students should not broadcast messages seeking answers to
elementary questions.
} Additionally, the use of "ASAP"
} was a bad idea.
}
} JQuinn
}
} PS: I sent Jenny Wang's message to her Chairperson and UG Program
Director.



Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



From daemon Mon Feb 11 08:39:37 2002



From: sterling stoudenmire :      sstouden-at-thelinks.com
Date: Mon, 11 Feb 2002 08:37:55 -0600
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


yes, "Education is a bueaucracy, learning is a biological activity!
The instructor serves as a resource with the ability to interact with and
to direct the inquiry of the student, but learning happens only at the
pleasure of the student. Sterling Stoudenmire, 1982.


At 10:27 AM 2/9/02 -0600, rnessler wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Mon Feb 11 10:01:05 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 11 Feb 2002 08:55:14 -0700
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

I have been getting emails with responses, but right now the numbers are
probably too low to be statistically significant. I'll wait until the end of
the week.

Also, please note: I had requested the emails to be sent directly to me,
because I did not want to overload the listserver. Again, please send the
responses to mb-at-soft-imaging.com.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================




From daemon Mon Feb 11 10:31:59 2002



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Mon, 11 Feb 2002 11:25:10 -0500
Subject: Re: EM: Exploding Kevex LN2 level sensor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The only thing I could think that could cause this would be ice
trapping LN2 in the sensor tube that then warmed up causing N2 gas
pressure high enough to pop the top. The design of our sensors have
the BNC connection on the top of the cap. Did the wires come out
with the metal top?



}
} Hi all,
}
} Just wanted to give a heads up for anyone with older Kevex LN2 cooled
} EDS systems. On Friday, our Kevex LN2 level sensor just exploded after
} 15years of service. Normally we remove the sensor (unplugged from the
} system) and place it horizontal in a special holder while we fill the
} LN2 then we dry it off and put it back in place. Today the metal top
} blew off and hit me in the leg. No injuries except the loud bang may
} have shaved a year or so off my life. Luckly, we had a second sensor
} on hand for replacement.
}
} If any one has a good explanation why the metal cover decided to
} tear itself away from the Styrofoam insulation after all these years
} we would like to hear from
} you.
}
} TIA
}
} Jon Ekman
} Florida State University
} Biological Science Imaging Resource
} 119 Bio Unit I, 4370
} Tallahassee, FL 32306
} tel: 850.644.6519
} fax: 850.644.0481


--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html


From daemon Mon Feb 11 10:55:54 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 11 Feb 2002 11:50:39 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Name-calling is not appropriate in this fourm. You own Mr. Quinn and the
entire list an apology

'"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I see we have the equivalent of a prison snitch in our midst ..or more
} appropriately in this case ..a school yard tattle-tale
} Jim Quinn wrote:
}
} } Don -
} }
} } College students should not broadcast messages seeking answers to
} elementary questions.
} } Additionally, the use of "ASAP"
} } was a bad idea.
} }
} } JQuinn
} }
} } PS: I sent Jenny Wang's message to her Chairperson and UG Program
} Director.
}
} Paul D. Nolan
} Electron Optics
}
} Alcan International Limited
} Kingston Research and Development Centre
} P.O.Box 8400, 945 Princess Street
} Kingston, Ontario K7L 5L9
}
} Tel: (613) 541-2066
} Fax: (613) 541-2134
} paul.nolan-at-alcan.com

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Mon Feb 11 11:06:01 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 11 Feb 2002 11:56:41 -0500
Subject: digging up OLD techiniques

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,
I have a client who is writing a grant and has "re-discovered" some
old techniques that could be very useful in her research. The
problem is, I'm having trouble finding a source or sources for the
reagents. Any ideas on where we could get the following?
Thoria Sol ( aqueous colloidal thorium, once sold by Polysciences. It
does not appear in their on-line catalog, nor in any of the other
catalogs I've checked) to be used for pinocytotic uptake to label
lysosomes. We could use ferritin, but that's so messy (in my hands,
anyway).
the full protocol for Gomori's method of acid phosphatase labelling.
I have the citation on order from Inter-Library loan (Arch.
Pathol.1941!!!) but don't know when it will come it.
Are the reagents still available? Has anyone out there done either
of these techniques? Any suggestions for alternates (preferably not
immuno)?
Thanks a million,
Lee


--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Feb 11 11:10:18 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 11 Feb 2002 17:04:00 +0000
Subject: Re: Answers to Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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Couldn't find a paper copy in our library so
I downloaded a synopsis from the web, along with the reviews.
Very, very funny, but not true. His dinosaurian heritage is showing,
and paper is definitely dead. Silicon is the world's commonest
material, not cellulose. Scientific publishing has ripped us all off.
We have ownership of the content, we did all the work, and they
charge us so much for the journals our libraries cannot afford the
subscriptions. Nor can universities afford the upkeep of the
libraries. There used to be departmental libraries here in every
department. Not any more. There was a time when Universities
could afford the upkeep of their physical establishments. Now
they're selling of the paintings to keep up with the maintenance.
The revolution is coming, and when it does paper journals will be
first against the wall. Twenty years from now, maybe sooner,
scientists will publish online, and the last 50 years of publishing
will be accessible online from anywhere in the world, and many
tertiary education courses will be distributed, campusless,
attended by students wherever they happen to live in the world.
And the educators? They'll be made by Intel ......

jmtc
Chris

} Gee, maybe this whole thread is just a Sociology experiment to gauge
} the response of a small group of specialists to a contentious issue! For
} my $0.02 ($0.033 Canadian), pick up a copy of "Silicon Snake Oil" by
} Cliff Stoll. Relevant reading, I think.
}
} Cheers,
}
} Jim
}
} --
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/~jehrman
}
}
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Mon Feb 11 11:18:51 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 11 Feb 2002 12:29:17 -0600
Subject: Re: EM: Exploding Kevex LN2 level sensor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,
#3, keeping in mind the possible limited sources available to the student
(high school students may not have *any* EM books available in their
library---so they need to be pointed to alternate information sources).
Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu

-----Original Message-----
} From: Mike Bode [mailto:mb-at-soft-imaging.com]
Sent: Friday, February 08, 2002 6:20 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Hello Listers:

Looks like there are different opinions out there. Before this turns into a
flame war, I would personally like to know what the general opinion is. I
therefore propose a poll. Just send me an email with nothing but one of the
following numbers in the subject line, and I will tally those votes and
provide a summary in a week (if I get enough emails):

1) Students should work through their assignments alone. Let's not support
laziness at all.
2) We should help them help themselves by pointing them to general
microscopy books.
3) We should take their questions and point them to specific microscopy
books dealing with their problems
4) We should encourage them to contact us offline so we can test their
sincerity and then either help them or not. This should be posted on the
server to avoid redundancy.
5) We should help them with their questions by trying to answer them in a
general way
6) We should go all out and answer their questions as best as we can.

I hope the statistics will not be skewed by a zillion students who send me
"6" as an answer ;-)

Any suggestions are welcome! I also promise not to misuse the email
addresses!

} } } please send the email to mb-at-soft-imaging.com { { { { { {

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Oakley, Jeff [mailto:oakleyj-at-rayovac.com]
Sent: Friday, February 08, 2002 11:46 AM
To: Microscopy-at-sparc5.microscopy.com


Tom,

You are correct, I am not an educator. I also agree with you whole
heartedly that when someone reads a text in search of information, they
learn a great deal more than they had planned on... It happens to me every
time I pick up a book in search of an answer to a question. I'm sure this
probably was the intent of the instructor.

The point I should have made in my previous post is that instead of shutting
someone down and "scolding" them (which is exactly what some of the listers
did) for what appeared to be a Cliff's Notes research method, the person
should have been guided to useful web pages or texts that would have made
them find the answers for themselves (which other posters did - kudos to
those).

It is possible to be helpful while at the same time not giving someone a
free ride.

I don't think my batteries are dead, Tom, I just think we are operating at
different voltages.

Jeff


-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Friday, February 08, 2002 9:26 AM
To: Oakley, Jeff
Cc: Microscopy-at-msa.microscopy.com


I vaguely recall the cap coming off of our Kevex cap years ago, but without
near so much excitement. I think it just worked loose. A check of the wires
and a little bit of epoxy and the cap was on again and has been fine since.

I can't remember if the dipstick was glued tightly into the Styrofoam plug.
If it was, I could see pressure building up under the cap. I don't think
the metal on ours was not glued all the way around.

Warren

At 11:25 AM 2/11/02 -0500, you wrote:

} The only thing I could think that could cause this would be ice trapping
} LN2 in the sensor tube that then warmed up causing N2 gas pressure high
} enough to pop the top. The design of our sensors have the BNC connection
} on the top of the cap. Did the wires come out with the metal top?
}
} }
} } Hi all,
} }
} } Just wanted to give a heads up for anyone with older Kevex LN2 cooled
} } EDS systems. On Friday, our Kevex LN2 level sensor just exploded after
} } 15years of service. Normally we remove the sensor (unplugged from the
} } system) and place it horizontal in a special holder while we fill the
} } LN2 then we dry it off and put it back in place. Today the metal top
} } blew off and hit me in the leg. No injuries except the loud bang may
} } have shaved a year or so off my life. Luckly, we had a second sensor
} } on hand for replacement.
} }
} } If any one has a good explanation why the metal cover decided to tear
} } itself away from the Styrofoam insulation after all these years we would
} } like to hear from
} } you.
} }
} } TIA
} }
} } Jon Ekman
} } Florida State University
} } Biological Science Imaging Resource
} } 119 Bio Unit I, 4370
} } Tallahassee, FL 32306
} } tel: 850.644.6519
} } fax: 850.644.0481



From daemon Mon Feb 11 13:32:00 2002



From: gary.m.brown-at-exxonmobil.com
Date: Mon, 11 Feb 2002 13:25:25 -0600
Subject: Re: staining of block copolymers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hussain,

To answer your question you need to know, among other things, the
specificity of interaction between RuO4 and the comonomers that comprise
your block copolymer. I suggest that you consult the literature for
reactivity of RuO4 with your materials. I usually start with Sawyer and
Grubbs book, Polymer Microscopy. I know that the first edition has
information that should help you.

Good luck,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



"Garber,
Charles A." To: MICROSCOPY BB
{cgarber-at-2spi.c {Microscopy-at-sparc5.microscopy.com}
om} cc:
Subject: staining of block copolymers

02/11/02 07:40
AM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hussain Hazrat wrote:
============================================
Question: Dear Sir,
I have stained a block copolymer isothermally crystallized samples
with RuO4 vapors. The block copolymers constitute poly(ethylene
oxide)(semicrystalline block) and poly(perfluorohexylethyl
methacrylate) as second block.
The structure is as
PFMA-b-PEO-b-PFMA
PEO block length is 227 EO units(20000 g/mol) and each PFMA block
was 5-7 PFMA units.
The TEM morphology that we got from this sample was showing a
layered or lamellar structure.There are dark lines and bright lines.
Now I dont know, which block has been stained with RuO4.
The second question is again the staining assigning problem, I got
TEM pictures from solutions of the same block copolymers, the samples
were stained again with the same dye. we got some spherical micelles
in the TEM picture, but we dont know, the staining blocks.
I hope u will consider answer my questions. I will be grateful
with my best regards
Hussain
========================================================
This kind of question is extremely difficult to "call" on the basis of
first
principles (or logic). You really need to see one or two "knowns", and
then
you can see which way the area percent of the dark vs. white changes.

If you are seeing some "spherical" features from solution precipitation,
and
it is just a guess, it might be that you are seeing segregation of
homopolymer into a more traditional kind of morphology for these kinds of
systems. At least when we have seen such features in other block copolymer
systems that was our conclusion.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================









From daemon Mon Feb 11 14:48:22 2002



From: Mike Jercinovic :      mjj-at-geo.umass.edu
Date: Mon, 11 Feb 2002 15:39:23 -0500
Subject: Post-Doc announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,
Here is an announcement for a post-doc opportunity here at UMass. Please
pass this along to anyone who you think might be interested.

thanks!
Mike Jercinovic


POST-DOC POSITION: MICROPROBE MONAZITE GEOCHRONOLOGY

The Department of Geosciences at the University of Massachusetts invites
applications for a Post-Doctoral Position in Geology. This two-year
position is specifically aimed at the rapidly emerging techniques of
electron microprobe analysis in geochronologic applications. UMass is
currently developing an optimized electron microprobe with Cameca, France
that is specifically designed for the exploration of techniques for age
mapping and dating of minerals (e.g. monazite, zircon) and trace element
analysis. This project includes optimization on virtually all fronts,
hardware, software, and technique development. One future direction will
involve synthesis and analysis of standards for calibration and background
measurement studies. The successful applicant will collaborate with UMass
Geosciences faculty (and associates) and with Cameca, and will be directly
involved with improvements and modifications to software, continued
evaluation of analytical techniques, synthesis and characterization of
standard materials, and application of the new techniques to geologic
problems. Applicants must have completed a Ph.D. in Geology, materials
science, or other physical science, with preference given to those with
significant experience in electron microprobe analysis, x-ray spectrometry,
materials microanalysis, and/or scientific programming.

Please send a letter of application, resume, and two reference letters to
Michael L. Williams, Dept. of Geosciences, University of Massachusetts, 611
North Pleasant Street, Amherst, MA 01003-9279. The University of
Massachusetts is an Equal-Opportunity Affirmative -Action Employer; women
and members of minority groups are encouraged to apply.

Review of applicants will begin March 15th; the position will remain open
until a successful candidate is identified.


****************
Michael J. Jercinovic
Assistant Professor
Department of Geosciences
University of Massachusetts
611 North Pleasant Street
Amherst, MA 01003-9297
E-Mail: mjj-at-geo.umass.edu
Phone: (413) 545-2431
http://www.geo.umass.edu/faculty/jercinovic.html

Electron Microprobe Laboratory
http://www.geo.umass.edu/probe/probe.html




From daemon Mon Feb 11 14:48:22 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Mon, 11 Feb 2002 15:50:08 -0500
Subject: Re: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For a look at what's coming down the path, see today's (Monday, February
11th) New York Times article on Foveon or that company's website. (I
have no financial interest in the company and was previously unaware of
them.)

John Twilley
Conservation Scientist

Michael Herron wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} All,
}
} OK so it is a given that consumer grade cameras have relativly poor low
} light performance. That said, are there cameras that have better than
} average lowlight performance? Are any of the consumer cameras capable
} of binning?
}
} Mike
}
}
} "Mardinly, John" wrote:
}
} }
} } John;
} } You are correct about the small CCDs of consumer digital cameras
} } have sever performance deficiencies due to their small pixel size. The F707
} } and other consumer digital cameras that use the same chip (2/3"-Nikon 5000
} } and Olympus E20N) suffer from noise even in visible light photographs. The
} } Nikon D1X and the new Canon EOS 1D(advertised 11.5 µm pixels) are
} } significant improvements, but cost $6000 just for the camera body! One
} } organization addressing this problem is
} } http://www.fredmiranda.com/Action_profilesPage/index.html where they discuss
} } and sell Photoshop plug-ins for noise reduction. Another digital camera site
} } I really like is:
} } http://www.imaging-resource.com/
} }



From daemon Mon Feb 11 15:03:29 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Sun, 10 Feb 2002 16:55:26 -0500
Subject: Wanted: Critical Point Freeze Drier and Sputter Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I am setting up a TEM/SEM lab and I need a vacuum coater and
critical point freeze drier for the SEM. I cannot afford new equipment and I will
consider any used but still functioning equipment, complete with manuals.

Thank you.

Greg Barclay

Dr.G.F. Barclay
Plant Science Unit, Dept. of Life Sciences
University of the West Indies
St. Augustine,
Trinidad and Tobago, West Indies




From daemon Mon Feb 11 15:42:49 2002



From: Ayten Celik :      celik-at-students.uiuc.edu
Date: Mon, 11 Feb 2002 15:36:21 -0600 (CST)
Subject: Suggestions on sapphire samples

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I'm trying to prepare tem samples from sapphire.
There is a thin metal film on the sapphire substrate, as well.
I don't have too much experience in this field so, I would greatly
appreciate any suggestions about preparing tem samples from sapphire.

Previously, I have prepared couple of Si samples but, sapphire seems to be
much harder and difficult to deal with.


Thank you very much,
Ayten C. Aktas.



From daemon Mon Feb 11 16:53:05 2002



From: cassel-at-biology.queensu.ca ()
Date: Mon, 11 Feb 2002 16:35:17 -0600
Subject: Ask-A-Microscopist: Recommendation Needed LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cassel-at-biology.queensu.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
February 11, 2002 at 15:05:08
---------------------------------------------------------------------------

Email: cassel-at-biology.queensu.ca
Name: Stephen Casselman

Organization: Queen's University

Education: Graduate College

Location: Kingston, Ontario, Canada

Question: I am involved in a project which is examining sperm in
fish, we will be using a video camera and a microscope to film motile
sperm. Much of this work will be done in remote locations. We are
interested in buying a new microscope that would able to handle
frequent transportation to these remote locations. Ideally the scope
would have a padded case specifically for it to be transported in.
We generally use a 40 X objective lense. Does such a durable scope
exist?

---------------------------------------------------------------------------


From daemon Mon Feb 11 16:53:12 2002



From: ekomarnicki-at-MacDermid.com
Date: Mon, 11 Feb 2002 16:35:31 -0600
Subject: Re: Carbon Coater Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Lou, have you looked at the Denton Desktop II as a backup or is that too
small for your needs?

Ed


From daemon Mon Feb 11 16:56:09 2002



From: zaluzec-at-microscopy.com
Date: Mon, 11 Feb 2002 16:52:03 -0600
Subject: Administrivia:Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

I think this thread has run it's course, let's bring it to a close.

Nestor
Your Friendly Neighborhood SysOp
-Waving Hi to Everyone from Sunny Sydney-




From daemon Mon Feb 11 16:58:37 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 11 Feb 2002 17:52:14 -0500
Subject: Re: digging up OLD techiniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Lee:

Leona Cohen-Gould wrote:

} Hi Listers,
} I have a client who is writing a grant and has "re-discovered" some
} old techniques that could be very useful in her research. The
} problem is, I'm having trouble finding a source or sources for the
} reagents. Any ideas on where we could get the following?
} Thoria Sol ( aqueous colloidal thorium, once sold by Polysciences. It
} does not appear in their on-line catalog, nor in any of the other
} catalogs I've checked) to be used for pinocytotic uptake to label
} lysosomes. We could use ferritin, but that's so messy (in my hands,
} anyway).

How about peroxidase followed by a DAB reaction? Shows pinocytosis well. The
method should be in Hyatt's book? Most histology texts (Weiss for one) will
have an EM of capillary endothelium with peroxidase showing the vesicles. It
may be a Karnovsky technique.

} the full protocol for Gomori's method of acid phosphatase labelling.
} I have the citation on order from Inter-Library loan (Arch.
} Pathol.1941!!!) but don't know when it will come it.

Gomori had a book out about 1950 or so, I suspect your library will have it.
Also, any edition of Lillie's "Histopathological Technique and Practical
Histochemistry" should do. Also John Kiernan's book should have it. Maybe
even Humason's book will have it.

} Are the reagents still available?

Fisher, Sigma, Aldrich.

} Has anyone out there done either
} of these techniques?

Not since grad school.


} Any suggestions for alternates (preferably not
} immuno)?
} Thanks a million,
} Lee
}
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Mon Feb 11 17:25:39 2002



From: Edward_Principe-at-amat.com
Date: Mon, 11 Feb 2002 15:16:06 -0800
Subject: Re: Answers to Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Now you got me going Jim,

I was just sitting back (but intrigued) but now I must share that I had a
very similar thought as your own.

Yeah, I imagined Jenny's actual assignment was to conduct a psychology
experiment, the question refined beautifully to elicit a response. Maybe
we should name this technique to gauge personality and opinion after
her....The Jenneric Response Factor. It might even be good for extra
credit on her report. anyway, it gave me quite a chuckle. Even if the
original intent was not to extract a slice of humanity, it will perhaps be
the most valuable life lesson. Fun stuff.

antiflame disclamer: I am not making light of anyone's serious and
passionate responses, just a perspective.

I also find it interesting which questions generate the most responses on
the listserver.

Regards,
Ed




"James M. Ehrman" {jehrman-at-mta.ca} on 02/11/2002 04:48:27 AM


To: Microscopy-at-sparc5.microscopy.com
cc:


Gee, maybe this whole thread is just a Sociology experiment to gauge
the response of a small group of specialists to a contentious issue! For
my $0.02 ($0.033 Canadian), pick up a copy of "Silicon Snake Oil" by
Cliff Stoll. Relevant reading, I think.

Cheers,

Jim

--
James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman







From daemon Mon Feb 11 19:03:27 2002



From: Edy Junop Widjaja :      ejw923-at-casbah.acns.nwu.edu
Date: Mon, 11 Feb 2002 18:55:19 -0600 (CST)
Subject: DM3 -> RAW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anybody help with file conversion?
I need to convert Gatan Format (.dm3) file from Digital Micrograph to RAW
format.

Thanks,

Edy Widjaja
Materials Science and Engineering
Northwestern University
reply to : e-widjaja-at-northwestern.edu
office : 847-491-7809 lab : 847-491-3281
http://www.numis.nwu.edu/internet/Staff/edy



From daemon Mon Feb 11 22:23:58 2002



From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Tue, 12 Feb 2002 17:14:54 +1300 NZDT
Subject: Re: digging up OLD techiniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On 11 Feb 02, at 11:56, Leona Cohen-Gould wrote:

} Any ideas on where we could get the following?
} Thoria Sol ( aqueous colloidal thorium, once sold by Polysciences. It
} does not appear in their on-line catalog, nor in any of the other
} catalogs I've checked) to be used for pinocytotic uptake to label
} lysosomes.

Hello Leona,

If you have it available, take a look at pp 116-117 (Section 3.5.5c
"Staining acidic carbohydrates with colloidal thorium dioxide") of
PR Lewis & DP Knight (1992) "Cytochemical staining methods for
electron microscopy", Volume 14 of the "Practical Methods in
Electron Microscopy" series.

They state that colloidal thorium was also known as Thorotrast,
and was formerly used for medical X-ray diagnosis but is now
difficult to obtain (it proved carcinogenic in the patients). Although
Lewis & Knight refer to Thorotrast as 'colloidal thorium', other
sources indicate that it is colloidal thorium dioxide - go to:
http://brighamrad.harvard.edu/Cases/bwh/hcache/161/full.html

Lewis & Knight offer a recipe for home-made colloidal thorium
dioxide as an alternative (CARE: RADIOACTIVE), and I have used
this exact method myself to stain acidic carbohydrates. It worked a
treat. Perhaps it would work in your application too? To make the
thorium dioxide I used a very old bottle of thorium nitrate from BDH -
a quick search of the WWW suggests it is no longer in their
catalogue. Perhaps safety and disposal concerns make it difficult
to obtain today. The EM labs on your campus might have a bottle
tucked away, if you still want to try it.

Back to the ferritin perhaps? ;-)


Regards

Stephen Edgar

Pathology Department
Faculty of Medicine & Health Sciences
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459


From daemon Mon Feb 11 23:16:38 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Tue, 12 Feb 2002 16:13:45 +1100
Subject: Re: digging up OLD techiniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Lee,

} Hi Listers,
} I have a client who is writing a grant and has "re-discovered" some
} old techniques that could be very useful in her research. The
} problem is, I'm having trouble finding a source or sources for the
} reagents. Any ideas on where we could get the following?
} Thoria Sol ( aqueous colloidal thorium, once sold by Polysciences. It
} does not appear in their on-line catalog, nor in any of the other
} catalogs I've checked) to be used for pinocytotic uptake to label
} lysosomes. We could use ferritin, but that's so messy (in my hands,
} anyway).

This is thorium dioxide, I think. Rather toxic, carcinogenic and
radioactive (alpha-emitter) too. It's in the Alfa Aeser catalogue
(www.alfa.com) under thorium (IV) oxide.


} the full protocol for Gomori's method of acid phosphatase labelling.
} I have the citation on order from Inter-Library loan (Arch.
} Pathol.1941!!!) but don't know when it will come it.
} Are the reagents still available? Has anyone out there done either
} of these techniques? Any suggestions for alternates (preferably not
} immuno)?


In "Plant Cell Biology: a Practical Approach" (1994), p. 62, is a full
protocol for doing this - I dug this up once before for a student. The
authors say there are several methods based on the Gomori reactions, and
this is one of them, and that a variety of substrates can be used, giving
different coloured products for LM. It looks very straightforward, I guess
you'd just have to be careful of artefacts. Ingredients: acetate buffer
(acetic acid + Na acetate), naphthol AS-MX phosphate, Fast Red TR, Tris-HCl
buffer, dimethylformamide.

If you're after a TEM protocol, "Electron Microscopy of Plant Cells" (1991)
gives details on pp. 125-131, recipe p. 161, in which case Pb is
precipitated in the reaction - also derived from Gomori. Many cautions
about artefacts. Ingredients: beta-glycerophosphate, acetate or
Tris-maleate buffer, lead nitrate solution. Or, can use cerium chloride in
acetate buffer preincubation, this buffer plus beta-glycerophosphate stain.

Don't think there would be a huge difference between plant and animal (incl
human!) cells....

cheers,

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475 or 61-0402 835 973
fax 61-2-6246 5000
email rosemary.white-at-csiro.au




From daemon Mon Feb 11 23:30:46 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 11 Feb 2002 21:27:00 -0800
Subject: Re: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



It is CMOS, not CCD, as I read it. This leads to
an entirely different venue.

gary g.


At 12:50 PM 2/11/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Feb 12 03:28:30 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Tue, 12 Feb 2002 10:19:23 +0100
Subject: RE : Nikon Coolpix vs Olympus C-3030 et al

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi all

I just want to point out two problems with either of these camera, and
with other too, from the cheapest to the most expensive :

1- Someone point out the probleme with the JPG compression. Right, but in
practice you are obliged to use the best resolution to be alowed to save
in TIFF format. If you need some memory to take a lot of pictures (dynamic
process), or if you don't need 1500x2000 pixel, you cannot have the tiff
format. And why don't these "new", "modern" camera use formats like JEPEG
2000 or SPIFF which let the choice of compressing (lossy or lossless) or
not ?

2- An other point, more discreet, is that the picture is adjusted to the
"best" dynamic scale before saving. The brighter pixel will be put to
"white", i.e. level 255 in 8 bit BW , and the darker to "black", i.e.
level 0, or something so. It's probably more sophisticated than that.
The important result is that you CANNOT make quantitative mesures on
brightness between different pictures. This is never said in commercial or
technical shits. You can choice between auto adjustement, normal (what
does it mean ?), more or less contrast or brightness, but you cannot put
that fonction off (see p 104 of the Coolpix 995 manual). Cheapest camera
have no settings, and do simply that adustement. More expensive one let
you choice "something" but don't say what they really do. We have our
Coolpix only since two month, so I had no time to try if there is a way to
bypass this problem. Has someone a experience about that ? We had soon the
same problem with the Fuji FinePix S1Pro. But we made only short tests
with it.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Tue Feb 12 03:42:08 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Tue, 12 Feb 2002 10:35:03 +0100
Subject: RE : self coating of EM viewing screen

Contents Retrieved from Microscopy Listserver Archives
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For those who may bee interested, I have such a receipt (which I sent to
Peter). Please ask off-line, an I can send it.

If some one else have one, I am too interrested. Our receipt works, but
has its deffects !


By the way, Edwards remarked about an other topic (EM quest. ...)

"I also find it interesting which questions generate the most responses on
the listserver."
..and which questions generate less (or no) responses.

I find it's difficult to know when it's useful to give an answer, when it
is better to give it off-line or on the list. Those who use the list since
years have perheps an advice about that.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Tue Feb 12 06:28:37 2002



From: Rachel Gouttebaron :      Rachel.Gouttebaron-at-umh.ac.be
Date: Tue, 12 Feb 2002 13:18:14 +0100
Subject: job position to be in charge of microscopy department (SEM - TEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The "MATERIA NOVA a.s.b.l." research Center, located in Mons - Belgium

recruits

1 Ph.D. or civil engineer - chemist or physicist

to set up and to be in charge of its Department of electronic microscopy
(SEM and TEM)

Initiated in 1995 in close collaboration with the University of Mons-Hainaut
and the Polytechnic Faculty of Mons, Materia Nova got its own identity in
2000 and has opened up largely its activities to the industrial world.
Materia Nova activities rely upon two main research fields: interfacial
aspects of materials and polymeric materials.

The main objectives of the Department of electronic microscopy can be
summarized as follows:
+ To reinforce the research Center capacity in materials analysis and
characterization : ion and electron spectroscopy - local probe microscopy -
ellipsometry - calorimetric methods - optical spectroscopy -
chromatographic methods - ...
+ To contribute to the formation/education in the field of surface and
interface chemistry.

Letter of application (preferably written in French) and C.V. have to be
addressed to:

Monsieur Joseph LEMINEUR - General Manager - MATERIA NOVA a.s.b.l., Parc
Initialis, Avenue Nicolas Copernic, B-7000 Mons, Belgium
e-mail: joseph.lemineur-at-umh.ac.be
Phone : ++32 (0)65 373800

To find more information concerning MATERIA NOVA" research center, please
visit : http://www.materia-nova.com


-----------------------------------------------------------
Rachel Gouttebaron
laboratoire d'Analyses de Surfaces par Spectroscopie Ionique et Electronique
(LASSIE)
Materia Nova
Parc Initialis
Avenue Nicolas Copernic
7000, mons, belgium
tel : +32 65 37 38 52
fax : +32 65 37 38 41
e-mail : rachel.gouttebaron-at-umh.ac.be



From daemon Tue Feb 12 07:04:37 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 12 Feb 2002 12:58:10 +0000
Subject: Colloidal gold probes in FESEM

Contents Retrieved from Microscopy Listserver Archives
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Dear All

I have been examining immunogold labelled cell and virus surfaces
in a Hitachi S4700 FEG SEM, and would be grateful for your help/
wisdom in interpreting what I see. In SE mode, labelled sites are
decorated with rounded blobs about 45-50nm diameter. Clear 10nm
gold particles are visible by YAG BSE imaging in the centre of
each blob. Blobs/gold are absent in unlabelled controls.
Presumably the blob represents the IgG shell surrounding the gold
particle? What is the "official" diameter for this shell? (I am trying
to estimate how much I have grown it by carbon coating).
At least as many labelled locations as are labelled with single gold
probes are labelled with binary or ternary probes. Mostly these are
pairs or triplets of overlapping blobs, with gold particle centres
separated by, typically, 22-24 nm. Occasionally two gold particles
appear to be very close together (i.e. touching or separated by 1-3
nm) at the centre of what appears to be a single blob. I assume
these latter represent pairs of gold particles that were cross-linked
by protein at the time of manufacture of the conjugate. Would that
be a fair conclusion? Intermediate spacings seem infrequent (I
haven't done any stats on this). Does anyone have a handle on
how close individual gold probes can approach each other before
being sterically excluded? This would have a bearing on the
quantitative relationship between label numbers and closely-spaced
epitopes. Is the answer by any chance somewhere in the 22-24 nm
region?

Many thanks in advance
Chris


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Tue Feb 12 08:13:05 2002



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Tue, 12 Feb 2002 09:04:52 -0500
Subject: Final Call for Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



UCF TEM Specimen Preparation Short Course - 2002

A Short Course With Emphasis on Recent Innovations in Tools and Methods

(Including tripod polishing, ion milling, and FIB techniques.)

Instructors:
Ron Anderson, IBM (retired);
Fred Stevie, NC State;
Lucille Giannuzzi, UCF


At the University of Central Florida (prior to the FL AVS/FL Society for
Microscopy Meeting)
Orlando, FL

Friday, Saturday and Sunday, March 8,9,10, 2002


for registration information please contact: Lucille Giannuzzi,
lag-at-mail.ucf.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Tue Feb 12 08:42:26 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 12 Feb 2002 08:42:40 -0600
Subject: Re: DM3 -> RAW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you have a Mac, try the latest version (or at least higher than
4.0.9) of Graphic Converter.
http://www.lemkesoft.com
shareware, an excellent graphics file-converter program with basic
image-manipulation tools.
For PCs, there's Irfanview
http://www.irfanview.com/english.htm
freeware, but there's a more complete shareware version, I *think*. I
don't know if Irfanview will convert Gatan to other formats, though.

Phil

} Can anybody help with file conversion?
} I need to convert Gatan Format (.dm3) file from Digital Micrograph to RAW
} format.
}
} Thanks,
}
} Edy Widjaja
} Materials Science and Engineering
} Northwestern University
} reply to : e-widjaja-at-northwestern.edu
} office : 847-491-7809 lab : 847-491-3281
} http://www.numis.nwu.edu/internet/Staff/edy
--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Tue Feb 12 08:47:17 2002



From: Stacy Darnell :      stacy-at-boeckeler.com
Date: Tue, 12 Feb 2002 07:41:49 -0700
Subject: 7th Annual Materials Microtomy Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This year we will be hosting the 7th annual RMC Materials Microtomy Short
Course in Tucson, Arizona from April 16-19, 2002.

For those of you residing in colder climes, Tucson has just the weather to
chase away the onset of those winter-time blues.

Join us, and our internationally renowned course faculty, in sunny Tucson to
participate in this unique event. This short course is designed
specifically for researchers in the field of materials science who wish to
gain exposure to advances in specimen preparation for electron microscopy.

Please email me at stacy-at-boeckeler.com to receive full details and a course
brochure.

Warm Regards,

Stacy Darnell
Administrative Assistant
RMC Products Division
Boeckeler Instruments, Inc.
stacy-at-boeckeler.com
4650 S. Butterfield Drive
Tucson, AZ 85714, USA
Tele: 520-745-0001
Fax: 520-745-0004



From daemon Tue Feb 12 08:53:21 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 12 Feb 2002 09:45:32 -0500
Subject: Re: digging up OLD techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers
Thanks to all of you who responded to my inquiry. As always, you
all have risen to the challenge, but after further discussion, my
client is going to try an approach using fluorescent labelling and
FRAP on the confocal. Neither of us wanted to deal with thorium if
we didn't have to.
If she does need the acid phosphatase for the EM. we'll do an AB-DAB method.

Lee
--
Lee Cohen-Gould
Electron & Optical Microscopy Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207


From daemon Tue Feb 12 09:32:31 2002



From: Dave Hall :      daveh-at-restechimage.com
Date: Tue, 12 Feb 2002 10:22:53 -0500
Subject: Re: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For a fairly easy read regarding CMOS vs. CCD technology, please check
the following PDF.

The article is a reprint from Photonics Spectra, January 2001. I'm sure
a few things may have changed, but the general ideas have held for a
while, and continue to do so.

http://www.dalsa.com/markets/Photonics_Spectra_CCDvsCMOS_Litwiller.pdf

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045


-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Tuesday, February 12, 2002 12:27 AM
To: John Twilley
Cc: MSA listserver



It is CMOS, not CCD, as I read it. This leads to
an entirely different venue.

gary g.


At 12:50 PM 2/11/2002, you wrote:
} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

From daemon Tue Feb 12 10:30:05 2002



From: William P. Sharp :      wsharp-at-asu.edu
Date: Tue, 12 Feb 2002 09:22:35 -0700
Subject: Balzers HPF Planchet Source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listers -

Can anyone put me in touch with a source for "clam shell" interlocking gold
alloy planchets used in the Balzers High Pressure Freezing apparatus - HPM
010? My search for the Swiss Precision company (our previous source)
yielded an address in Palo Alto CA (908 Industrial Drive) and a phone
number that proved to be that of a private home. The number associated with
the company that was given on a web site that accurately described the
planchets as Craig Type and as a product of that company was 650-493-0440.

Thanks for any help you can give me in tracking these folks down.

Regards,
Bill Sharp
William P. Sharp
Arizona State University
Dept. Plant Biology, box 871601
Tempe, AZ 85287-1601
Phone - (480)-965-3210
Fax - (480)-965-6899



From daemon Tue Feb 12 10:53:01 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 12 Feb 2002 16:46:03 +0000
Subject: Paper vs the net

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


By the way, my posting yesterday about paper vs the net was
more than just a rant. The following urls may give you some idea
where I am coming from:

http://www.lib.ed.ac.uk/lib/news/curldoc.html
http://www.arl.org/sparc

Best wishes
Chris Jeffree

==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Tue Feb 12 11:28:52 2002



From: Rick Mott :      rickmott-at-alumni.princeton.edu
Date: Tue, 12 Feb 2002 12:35:22 -0500
Subject: Re: The true intent of the question now revealed: Answers to Questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Edward_Principe-at-amat.com"-at-sparc5.microscopy.com wrote:

}
} "James M. Ehrman" {jehrman-at-mta.ca} on 02/11/2002 04:48:27 AM
} ... pick up a copy of "Silicon Snake Oil" by
} Cliff Stoll. Relevant reading, I think.
}

I've met Cliff Stoll. He's a very interesting character,
and an archetypal hacker (in the classic favorable sense
of technically proficient and inventive, before the word
was co-opted by the ignorant press to mean people who break
into computer systems). That someone with his background
should be so negative about on-line communities is unusual.
His "Cuckoo's Egg" book on computer security makes a dry
subject very entertaining.

For another perspective, look at "The Cathedral and the
Bazaar" by Eric Raymond. While the nominal subject is
the open-source and Linux paths for software development,
he is a keen observer of the behavior of on-line groups.
His key insight is that, rather like academia but much
less structured, reputation is the coin of the realm
online as well, which is why so many people will spend
so much time in what seems like unproductive activity
in traditional career terms. Posts pile up as "publications"
of a sort. Peer review is immediate and severe, as we've
seen. :)

Rick Mott


From daemon Tue Feb 12 11:33:30 2002



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 12 Feb 2002 12:28:55 -0500
Subject: New England Society for Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The New England Society for Microscopy announces that the next meeting of
the Society will be held at 6.00 p.m. on Tuesday March 12th. 2002 at the
Lexington Laboratory of Raytheon Corporation. The theme of the program
will be Scanned Probe Microscopies.

Full details of the meeting, including the program, and information about
the New England Society may be found on the Society's Web pages, located at
http://www.msa.microscopy.com/MSALAS/NESM/NESMHome.htm

All interested persons are welcome, and invited to attend.

Tony Garratt-Reed
President Elect


* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*




From daemon Tue Feb 12 12:32:28 2002



From: Gordon Nord :      gnord-at-mindspring.com
Date: Tue, 12 Feb 2002 13:24:38 -0500
Subject: NYC Source for TEM Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

We need a New York City source for Kodak Electron Microscope film 4489
(3.25" x 4").

Arkin Medeo was our previous source but their phones are disconnected
and we can't find them.

Thanks,
Gordon Nord

--
Gordon L. Nord Jr.
Environmental Sciences Laboratory
Brooklyn College




From daemon Tue Feb 12 12:48:33 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Tue, 12 Feb 2002 10:37:57 -0800
Subject: Suggestions on sapphire samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ayten:

We have a few application notes on preparing sapphire for TEM that you
can download from our website. Go to www.southbaytech.com and navigate
to "Applications Support". Then select "Application Notes". You are
looking for Application Notes 34 and 55. Number 34 deals with Tripod
Polishing and number 55 deals with MicroCleaving.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David

Ayten Celik wrote:


------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.

Dear all,

I'm trying to prepare tem samples from sapphire.
There is a thin metal film on the sapphire substrate, as well.
I don't have too much experience in this field so, I would greatly
appreciate any suggestions about preparing tem samples from sapphire.

Previously, I have prepared couple of Si samples but, sapphire seems
to be
much harder and difficult to deal with.

Thank you very much,
Ayten C. Aktas.

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Tue Feb 12 15:32:08 2002



From: Ayten Celik :      celik-at-students.uiuc.edu
Date: Tue, 12 Feb 2002 15:19:18 -0600 (CST)
Subject: More detail..Re: Suggestions on sapphire samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I will be preparing both plane view and cross sectional samples. My main
concern is to keep the metal film in its initial state throughout the
sample preparation steps (as much as possible). The metal films are
generally 200-300 Angstrom thick.

Can dimpler alter/damage the thin film?

Second problem is the hardness of the sapphire. Do I have to use diamond
products to reduce the thickness of the sapphire substrates? The
substrates are 0.5 mm to start with.

For grinding and polishing few different type of equipment are available
(from hand polising to automatic polishing machines). Getting access to
equipment is not the main concern.


Thanks
Ayten C. Aktas


On Mon, 11 Feb 2002, Ayten Celik wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear all,
}
} I'm trying to prepare tem samples from sapphire.
} There is a thin metal film on the sapphire substrate, as well.
} I don't have too much experience in this field so, I would greatly
} appreciate any suggestions about preparing tem samples from sapphire.
}
} Previously, I have prepared couple of Si samples but, sapphire seems to be
} much harder and difficult to deal with.
}
}
} Thank you very much,
} Ayten C. Aktas.
}
}




From daemon Tue Feb 12 16:33:42 2002



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu
Date: Tue, 12 Feb 2002 16:28:49 -0600
Subject: Polypyrrole films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
Does anyone have experience using polypyrrole films in lieu of
formvar/collodion in TEM applications? They sound like the best thing since
sliced bread, as they are very thin and conductive. Too good to be true?
Comments?
Randy


From daemon Tue Feb 12 17:38:59 2002



From: gerard.d.gagne-at-abbott.com
Date: Tue, 12 Feb 2002 17:30:27 -0600
Subject: Summer Microscopy Internships at Abbott Laboratories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Forwarded by Gerard D Gagne/LAKE/PPRD/ABBOTT on 02/12/02 05:28 PM -----

Jane A
Fagerland To: Gerard D Gagne/LAKE/PPRD/ABBOTT-at-ABBOTT
cc:
02/12/02 Subject: internships
05:25 PM








The Department of Microscopy and Microanalysis at Abbott Laboratories will
sponsor two summer internships for students interested in experiencing
microscopy in the healthcare industry. One internship will be in Biological
Microscopy, and one will be in Materials/Surface Science.

The department houses state-of-the-art equipment including field emission and
environmental scanning electron microscopes with energy dispersive x-ray
spectroscopy, transmission electron microscopes, several types of fluorescence
technologies (including conventional fluorescence, confocal, and flow
cytometry), light microscopy (including polarized light and interference
contrast), laser capture microdissection, and all ancillary preparatory
equipment such as microtomes, cryostat, and evaporative coaters. Surface
analytical capabilities include x-ray photoelectron spectrometry and atomic
force microscopy. We have several image analysis systems, including MetaMorph
and AnalySIS. The department consists of nine microscopists with expertise in
cell biology, materials analysis, surface chemistry, ultrastructural
pathology, in vitro toxicology, and all the associated technical skills.

For details about summer internships at Abbott and for application forms and
instructions, please visit www.abbott.com and follow the instructions there.
Please note: the deadline for applications is March 1!

IN ADDITION, please send a copy of your resume with cover letter either by
snail mail or e-mail to:

Jane A. Fagerland, Ph.D.
Abbott Laboratories
D-R45M/AP31
200 Abbott Park Rd.
Abbott Park IL 60048-6202

jane.a.fagerland-at-abbott.com




From daemon Tue Feb 12 17:59:22 2002



From: JLCastner-at-aol.com
Date: Tue, 12 Feb 2002 18:52:56 EST
Subject: Coolpix 995 and Compound Microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Would anyone using a Nikon Coolpix 995 digital camera on a Nikon Eclipse
series compound microscope please contact me regarding your experiences with
this setup. I'd like to know the pro's and con's of working with this system
to produce publishable images.

Thanks very much.

Jim Castner
Gainesville, FL
jlcastner-at-aol.com


From daemon Tue Feb 12 18:32:16 2002



From: Tom Malis :      malis-at-nrcan.gc.ca
Date: Tue, 12 Feb 2002 19:25:53 -0500
Subject: Re: More detail..Re: Suggestions on sapphire samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mary, find someone with a focused ion beam (FIB) system that is willing to
try non-semiconductor applications, otherwise it will take you just this
side of forever to produce both plan view and cross section specimens.
Failing that, you have a real challenge on your hands, I'm afraid. If you
can make do with cross sections only, do a Listserver search on tripod
polishing.

Tom Malis
Materials Technology Lab
Ottawa, Canada

} From: Ayten Celik {celik-at-students.uiuc.edu}
} Date: Tue, 12 Feb 2002 15:19:18 -0600 (CST)
} To: {Microscopy-at-sparc5.microscopy.com}
} Subject: More detail..Re: Suggestions on sapphire samples
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi,
}
} I will be preparing both plane view and cross sectional samples. My main
} concern is to keep the metal film in its initial state throughout the
} sample preparation steps (as much as possible). The metal films are
} generally 200-300 Angstrom thick.
}
} Can dimpler alter/damage the thin film?
}
} Second problem is the hardness of the sapphire. Do I have to use diamond
} products to reduce the thickness of the sapphire substrates? The
} substrates are 0.5 mm to start with.
}
} For grinding and polishing few different type of equipment are available
} (from hand polising to automatic polishing machines). Getting access to
} equipment is not the main concern.
}
}
} Thanks
} Ayten C. Aktas
}
}
} On Mon, 11 Feb 2002, Ayten Celik wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} }
} } Dear all,
} }
} } I'm trying to prepare tem samples from sapphire.
} } There is a thin metal film on the sapphire substrate, as well.
} } I don't have too much experience in this field so, I would greatly
} } appreciate any suggestions about preparing tem samples from sapphire.
} }
} } Previously, I have prepared couple of Si samples but, sapphire seems to be
} } much harder and difficult to deal with.
} }
} }
} } Thank you very much,
} } Ayten C. Aktas.
} }
} }
}
}



From daemon Tue Feb 12 20:08:32 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 12 Feb 2002 16:00:02 -1000 (HST)
Subject: PBL in education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, All-

I had a couple of people ask me about the PBL curriculum at the UH medical
school as part of the thread about answering the questions from the
students at Georgia Tech. For those who are interested, a statement about
the philosophy can be found by visiting http://hawaiimed.hawaii.edu
and clicking on the Medical Education link.

I'm not associated with the medical school, but the med students I've know
have been very happy with it. However, I haven't had to visit any of them
in an emergency, yet...!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Feb 13 04:05:39 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Wed, 13 Feb 2002 10:55:22 +0100
Subject: Re: Nikon Coolpix vs Olympus C-3030 et al.

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I can only give a personal opinion based on my own experience with
fluorescence microscopy. For low-light applications I personally prefer
intensified cameras like the Isis-3 from Photonic Science (
http://www.photonic-science.ltd.uk/zzt_isis3.html ). These cameras allow
you to work in extreme dim working conditions, which will keep your
biological assay simple. You will not need to use several intensifying
steps by using multiple layers of antibodies in an assay to get enough
signal to get over the detection limit of your camera. These cameras
will also allow you to keep up the speed of your image acquisition as
you will not need to integrate the signal and thereby slow down the
image capture process.

The main disadvantage of this approach is the relatively low S/N ratio
in your images, but this can be compensated for with the proper digital
filters and analysis algortihms.

When using cameras without image intensifier, you will probably need to
integrate the signal over a certain period of time, which will slow down
the acquisition proces and won't allow you to analyse "fast" dynamic
processes. The benefit of this approach is a better S/N ratio than with
intensified cameras and subsequent a more simplified analysis.

One of the issues under consideration is however the size of the
CCD-elements on the camera, which are a measure of how much photons can
be accumulated. There is an inverse relation between the size of the
CCD-elements and the Nyquist sampling rate. For a given resolution (~
N.A.) of a microscope, you will need to magnify you image more if the
CCD-elements are bigger than with smaller CCD-elements on the chip. You
need to match the resolution of your microscope to that of your camera
to obtain optimal results.

But there is also an inverse relation between the magnification and the
amount of light (~I) hitting your camera ( I am not quite sure anymore,
but it is something like this: I = N.A.^4 / M^2). So, the more you
magnify your image, the more light you lose. Low-light fluorescence
microscopy with immersion-oil lenses (high N.A.) and intensified cameras
gives exciting results, but is sometimes a bit messy ;-)

Best regards,

Peter Van Osta


--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621



Michael Herron wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} All,
}
} OK so it is a given that consumer grade cameras have relativly poor low
} light performance. That said, are there cameras that have better than
} average lowlight performance? Are any of the consumer cameras capable
} of binning?
}
} Mike


From daemon Wed Feb 13 06:01:46 2002



From: paques-at-nizo.nl
Date: Wed, 13 Feb 2002 12:52:45 +0100
Subject: Food Structure & Functionality Forum short course 2002: second

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Wed Feb 13 07:59:55 2002



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Wed, 13 Feb 2002 08:45:07 -0500
Subject: re: NYC Source for TEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,

Any local Kodak distributor should be able to order the 4489 film. It takes
2-4 weeks for delivery. They probably will not keep it in stock, however
most places should be a able to order it. You could try Kodak's main office
Rochester, NY for names and phone numbers of local distributors.
Good Luck.

Jackie Garfield
Lifecell Corporation


From daemon Wed Feb 13 07:59:55 2002



From: Peter Steele :      STEELEP-at-allkids.org
Date: Wed, 13 Feb 2002 08:50:13 -0500
Subject: Cameras, Brightfield, DN100, DXC390, Micropublisher

Contents Retrieved from Microscopy Listserver Archives
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I am assessing vastly different systems that use the Nikon DN100, the Sony DXC390, or the Q-Imaging Micropublisher cameras for capturing bright field microscopy (mainly frozen biopsies). I am seeking any comments or experiences (benefits/limitations) about any of these three cameras (or others). For example, ease of use, software friendliness, quality, robustness, . . .

Please feel free to send responses offline, if they are not appropriate for the list.

Thank you,

Peter O. Steele, Ph.D.
Pathology and Laboratory Medicine
All Children's Hospital
St. Petersburg, Fl


E-mail: steelep-at-allkids.org



From daemon Wed Feb 13 08:40:58 2002



From: Pea9000-at-aol.com
Date: Wed, 13 Feb 2002 09:34:08 EST
Subject: Re: Polypyrrole films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,
Of course it's too good to be true...for now. We are currently working on new ways to synthesize polypyrrole (and polyaniline, also a conducting polymer). Unfortunately, these and related conducting polymers are sensitive to temperature, and only conduct if doped properly (in certain pH ranges with certain dopants). Plus, the films themselves are quite brittle and somewhat difficult to manipulate.
Like all new materials, though, it is a promising technology, and applications such as the one you mentioned is a definite possibility. File the provisonal patent now!

Paul E. Anderson
Post-doctoral Research Associate
Northeastern University
Department of Chemistry
102 Hurtig Hall
Boston, MA 02115


From daemon Wed Feb 13 08:57:57 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 13 Feb 2002 09:49:59 -0500
Subject: Re: NYC Source for TEM Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,
I get my 4489 from VWR and Electron Microscopy Sciences. The Med.
School contracts with VWR, so we get a bit of a discount from them,
but sometimes they don't have it in stock.
Lee
--
Lee Cohen-Gould
Electron & Optical Microscopy Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207


From daemon Wed Feb 13 11:13:03 2002



From: paques-at-nizo.nl
Date: Wed, 13 Feb 2002 18:03:43 +0100
Subject: short course announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Short Course
?Specific localisation methods and microscopy in Food Research.?

Sunday May 5th, 2002, 8:00 am - 6:00 PM
Electron Microscopy Centre, McGill University
Montréal, Québec, Canada

Sponsored by the Food Structure & Functionality Forum Division of the AOCS

Short Course Organizer:
Marcel Paques, Wageningen Centre for Food Sciences / Unilever R&D Vlaardingen,
The Netherlands

Spreadability, shelve life, fracture behaviour, and creaminess are examples of
functional properties of food products. These properties originate from the
microscopic structure of products. Specific localisation techniques and
microscopy are powerful tools to facilitate intelligent modification of
ingredient composition or processing to obtain targeted food product properties.
The short course is aimed at R&D personnel in the Foods area (fundamental
research, innovation, and product development). The course consists of lectures
and an intensive hands-on practical section providing participants with
sufficient basic knowledge and skills to set-up and implement the methods in
their own work. Registered participants are encouraged to submit
application-related questions to the instructors by email prior to the short.
In addition a personal consultation is offered to each participant scheduled (by
appointment) during the AOCS Annual Symposium 2002 in the days directly
following the short course. Contact Marcel Paques at paques-at-nizo.nl with
questions.


Programme topics include:

· Pre-course consultation (by email) with participants to ensure the course
content is relevant and applicable to participants? interests
· Introduction to specific localisation methods and principles
· Localisation strategies, marking options, and imaging approaches
· Experimental set-up, preparation and incubation procedures
· Demonstration examples
· Hands-on practical sessions
· Tailored help and advice during private consultation session following
short course

Course contributors:
Jan Leunissen (Aurion: immuno Gold Reagents & accessories, custom labeling,
The Netherlands)
Hong Yi (Emory Neurology Microscopy Core Laboratory, Emory University
Department of Neurology, USA)
Marcel Paques (Wageningen Centre for Food Sciences/Unilever Research
Vlaardingen, The Netherlands)

Registration Fee: $375. The registration fee includes complete course materials,
continental breakfast, lunch, two refreshment breaks, and transportation to and
from McGill University.

Space is limited so register online today!
http://www.aocs.org/meetings/am2002/fscourse.htm

This electronic message is sent by NIZO food research to its business partner
and may contain confidential information only to be used by the client. The
contents may not be used by, copied or revealed to any other person than the
addressee.
In case this message was mistakenly addressed to you, please return the message
to info-at-nizo.nl or call +31 (0)318 659 511




From daemon Wed Feb 13 11:23:18 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 13 Feb 2002 11:17:48 -0600
Subject: NYC Source for TEM Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,

If I'm not mistaken, National Graphic Supply in Albany carries this film in stock. In the past, George Laing has been our contact there for photographic supplies. The number I have for them is 1-800-223-7130, and George's extension is 3109. I haven't been in touch with them for awhile, but George has always been very helpful in the past.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Gordon Nord [mailto:gnord-at-mindspring.com]
Sent: Tuesday, February 12, 2002 12:25 PM
To: Microscopy-at-MSA.Microscopy.Com


Dear List,

We need a New York City source for Kodak Electron Microscope film 4489
(3.25" x 4").

Arkin Medeo was our previous source but their phones are disconnected
and we can't find them.

Thanks,
Gordon Nord

--
Gordon L. Nord Jr.
Environmental Sciences Laboratory
Brooklyn College




From daemon Wed Feb 13 11:36:07 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 13 Feb 2002 09:30:20 -0800
Subject: More detail..Re: Suggestions on sapphire samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ayten;
I prepared some silicon on sapphire samples at Lockheed a number of
years ago using dimple and ion mill techniques, but it is extremely
challenging. The sapphire is nearly as hard as diamond, so the polishing
rates are extremely slow. If you get aggressive, then it cracks. If you want
to look at a metal film on top of the sapphire, you should cap it with SiO2,
or it will be corroded/eroded and destroyed by the time the sapphire gets
thin. I never tried plan views, but they should be similarly challenging.
You will also find ion milling rates to be extremely slow, and must be done
with the beam crossing the sapphire first, using rocking of the sample
(never rotating) or the metal film will be lost. Tom Malik's suggestion of
finding a FIB should be taken very seriously.

John Mardinly
Intel



-----Original Message-----
} From: Ayten Celik [mailto:celik-at-students.uiuc.edu]
Sent: Tuesday, February 12, 2002 1:19 PM
To: Microscopy-at-sparc5.microscopy.com



Hi,

I will be preparing both plane view and cross sectional samples. My main
concern is to keep the metal film in its initial state throughout the
sample preparation steps (as much as possible). The metal films are
generally 200-300 Angstrom thick.

Can dimpler alter/damage the thin film?

Second problem is the hardness of the sapphire. Do I have to use diamond
products to reduce the thickness of the sapphire substrates? The
substrates are 0.5 mm to start with.

For grinding and polishing few different type of equipment are available
(from hand polising to automatic polishing machines). Getting access to
equipment is not the main concern.


Thanks
Ayten C. Aktas


On Mon, 11 Feb 2002, Ayten Celik wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear all,
}
} I'm trying to prepare tem samples from sapphire.
} There is a thin metal film on the sapphire substrate, as well.
} I don't have too much experience in this field so, I would greatly
} appreciate any suggestions about preparing tem samples from sapphire.
}
} Previously, I have prepared couple of Si samples but, sapphire seems to be
} much harder and difficult to deal with.
}
}
} Thank you very much,
} Ayten C. Aktas.
}
}




From daemon Wed Feb 13 12:32:40 2002



From: tartenon-at-netscape.net
Date: Wed, 13 Feb 2002 13:25:05 -0500
Subject: RE: RE : Nikon Coolpix vs Olympus C-3030 et al

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One possible way to bypass automatic adjustment with the coolpix 995 is to set it to manual operation,and turn off the awb (auto white balance).

Regards

Alfredo

Faerber Jacques {Jacques.Faerber-at-ipcms.u-strasbg.fr} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




__________________________________________________________________
Your favorite stores, helpful shopping tools and great gift ideas. Experience the convenience of buying online with Shop-at-Netscape! http://shopnow.netscape.com/

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From daemon Wed Feb 13 12:56:41 2002



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Wed, 13 Feb 2002 13:51:55 -0500
Subject: Re: NYC Source for TEM Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,
Arkin Medo changed their number.
The new number for Arkin Medo is (718) 445-4000.
Frank

At 01:24 PM 2/12/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 13 13:23:21 2002



From: Baggethun, Paul :      Paul.Baggethun-at-alcoa.com
Date: Wed, 13 Feb 2002 14:15:13 -0500
Subject: SEM/TEM: Measurement of oxide film thickness by WDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Experts,

I am searching for references on measurement of oxide film thickness on
metals by WDS in the SEM or TEM (as an alternative to XPS/Auger). Does
anyone have advice on the "best" journal papers on this topic?

Sincerely,
Paul Baggethun
==================
Alcoa Technical Center
Alcoa Center, PA 15069
(724) 337-1760
==================



From daemon Wed Feb 13 14:19:39 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 13 Feb 2002 15:37:03 -0500
Subject: In the dark about my Densi-timer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ayten,

One of my former students developed a very nice technique to make both plan
view and cross sectional samples based on the Tripod Polisher from South Bay
Technology. It is a high angle polishing technique that works particularly
well for samples where the film and the substrate have very different
hardness and milling rate. With this technique you can also monitor the
actual thickness of the thinned part of the sample. We have successfully
used the technique to make samples of sapphire as well as Si (particularly
good for this technique), LaAlO3, SiC, GaAs, SrTiO3, etc. The reference for
the description of the technique is

"The Concept of High Angle Wedge Polishing and Thickness Monitoring in TEM
Sample Preparation," Hao Li, and L. Salamanca-Riba, Ultramicroscopy 88,
171-8 (2001).

If you want to see TEM images of samples made with this technique let me
know and I will e-mail you some off line.

Good luck,

Lourdes Salamanca-Riba




----- Original Message -----
} From: Ayten Celik {celik-at-students.uiuc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, February 12, 2002 4:19 PM


Hi Listers,

This appeal goes out to all the old timers out there who remember back when all pictures were created in the darkroom (I still think film and paper give a better picture and you techies out there can't change my mind ;-) ).

I have an old Densi-timer model PTM-4A made by Lektra Laboratories, Inc. It is a swell little thing that helps determine the exposure time for a print by using some sort of magic using a red light pointed at a medium gray tone of the negative and several knobs geared towards what grade of paper is being used.

Now I'm an old hand a printing and am used to just kind of knowing what time and what kind of paper to use to make a pretty picture, but my young helpers aren't. Does anyone know of a similar type device that is new & modern? This puppy has tubes and is on it's last legs.

I have money buring a hole in my pocket and Uncle Sam will take it back if I don't spend it soon. Any suggestions as to where to look and what's out there are greatly appreciated. Folks I talked to out here just gave me a blank stare.


Help keep me in the dark(room),

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Wed Feb 13 15:33:37 2002



From: Joel Mancuso :      mancuso-at-biology.utah.edu
Date: Wed, 13 Feb 2002 14:23:46 -0700
Subject: Repost jobs please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Wed Feb 13 16:25:18 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 13 Feb 2002 14:18:50 -0800
Subject: Summer Internship at Intel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are pleased to announce the availability of a Summer Internship at Intel
Santa Clara for the Summer of 2002. The successful candidate will develop
techniques for TEM Electron Tomographic 3D imaging of microelectronic
structures. This work will involve everything from focused ion beam specimen
preparation, imaging, reconstruction, rendering, and presentation via
digital movies. The ideal candidate should be a graduate student in
Materials Science, Physics, or equivalent, with experience in general theory
and use of transmission electron microscopes, and be particularly facile
with PCs and image processing. Unix/Linnux/SG experience would be a great
asset. This work is being conducted in collaboration with the Agard
Laboratories at UCSF, and frequent travel between Santa Clara and San
Francisco will be required. Non-citizen candidates must have a Green Card or
other legal right to work in the United States. Interested candidates should
contact me and submit resumes as soon as possible either by e-mail, phone,
or at the below mailing address. Thank you.

John Mardinly
John.Mardinly-at-Intel.com
Intel Corp.
2200 Mission College Blvd. SC9-7
Santa Clara, CA 95054
Desk: 408-765-2346
Pager: 408-322-6490


From daemon Wed Feb 13 16:30:12 2002



From: hina-at-ohio.edu ()
Date: Wed, 13 Feb 2002 16:25:07 -0600
Subject: Ask-A-Microscopist: osmolarity and TEM fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (hina-at-ohio.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
February 13, 2002 at 11:24:14
---------------------------------------------------------------------------

Email: hina-at-ohio.edu
Name: Sarah Hina

Organization: Ohio University

Education: Graduate College

Location: Athens, OH USA

Question: I work in a lab where we are investigating hair bundles of
the utricle (inner ear). I have a question regarding osmolarity and
TEM fixation. We are using the De Groot fixative, which contains 3%
Glutaraldehyde, 2% Paraformaldehyde, 1% Acrolein, and 2.5% DMSO in
0.8M Sodium Cacodylate-HCL buffer. The total osmolarity is 1149
mmol/kg, considerably more hypertonic than the extracellular fluid
(about 280 osmoles). I have read that glutaraldehyde and
formaldehyde do not contribute significantly to the effective osmotic
pressure of the fixative. Is this true? Does anyone know if
acrolein and DMSO contribute to the effective pressure? We measured
the osmolarity of our buffer alone, and it was a more reasonable 415
mmol/kg. Is this the more important value? Also, we are fixing by
way of vascular perfusion, which according to what I have read,
requires a more hyperosmolar solution. I would very much appreciate
any comments or suggestions. Thanks!

---------------------------------------------------------------------------


From daemon Wed Feb 13 17:02:52 2002



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Wed, 13 Feb 2002 17:58:04 -0500
Subject: NYC Source for TEM Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,
Although not in NYC, National Graphic Supply of Albany keep EM film in
stock and can ship the same day of order. They give a special discount price
for Colleges and Universities.
Phone: 1-800-223-7130 or check their web site at
http://www.ngscorp.com

Disclaimer: I have no commercial interest in the firm. They have supplier
us satisfactorily for many years .
Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu

-----Original Message-----
} From: Gordon Nord [mailto:gnord-at-mindspring.com]
Sent: Tuesday, February 12, 2002 1:25 PM
To: Microscopy-at-MSA.Microscopy.Com


Dear List,

We need a New York City source for Kodak Electron Microscope film 4489
(3.25" x 4").

Arkin Medeo was our previous source but their phones are disconnected
and we can't find them.

Thanks,
Gordon Nord

--
Gordon L. Nord Jr.
Environmental Sciences Laboratory
Brooklyn College






From daemon Wed Feb 13 19:38:15 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 13 Feb 2002 17:33:38 -0800
Subject: Re: In the dark about my Densi-timer

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I use an X-Rite densitometer for critical neg analysis
and printing. Sorry, not TEM or SEM negs. But the
trick is to collect the D of highlights and shadows and
then determine the total D range of the neg. This then
leads to the optimal contrast factor for print paper and
how much dodging and burning is necessary for a
final print.

If you scan the original neg at high rez, you can get
a good idea of its range from PS histogram. Then,
adjust accordingly.

gary g.




At 12:37 PM 2/13/2002, you wrote:
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From daemon Thu Feb 14 02:36:51 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Thu, 14 Feb 2002 09:26:06 +0100
Subject: JPEG, TIFF and nonlinearity of a camera

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Hi,

Adaptive behaviour and non-linearity of the response of a camera are
common problems in digital microscopy. Most of the cameras I prefer to
use have the option to disable this adaptive behaviour, but also the
framegrabber/digitizer can have an adaptive response. For quantitative
microscopy I prefer to disable the adaptive behaviour on both the camera
and the framegrabber.

In general it is best to calibrate the response of a camera and
framegrabber with a "dark" image and a "white" image which corresponds
with the dynamic range of the microscope image(s) you intend to acquire
after disabling the adaptive behaviour. Afterwards you can use these
images for calculation of the optimal dynamic range.

For JPEG and TIFF file formats, it is generally a bad idea to use JPEG
compression for images that need to be analysed afterwards, certainly
for color images as this will introduce artefacts. For B/W images, JPEG
compression can be used, depending on the kind of analysis that follows
or if the images are only meant for displaying and printing. In my
opinion it is no use to acquire an image with a 100X high N.A.
oil-immersion lens and afterwards destroy the fine detail with
JPEG-compression. One of the advantages of JPEG-compression however is
that there are hardware compression engines available, which allow you
to do the compression in real-time which might be useful if speed is an
issue.

For images that are meant to be analysed, I personally prefer lossles
LZW-compression on TIFF images, certainly for color images. It gives you
less data-compression than is possible with JPEG-compression, but the
quality of the iamges is better preserved.

In general one needs to be aware of the difference between lossy and
lossles compression algorithms and what the images are meant for in the
end.

Best regards,

Peter

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621


From daemon Thu Feb 14 06:23:43 2002



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From: root-at-rss1.rz.uni-regensburg.de
Date: 14 Feb 2002 13:17:27 +0100
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From: root-at-rss1.rz.uni-regensburg.de
Date: 14 Feb 2002 14:15:11 +0100
Subject: Haengengebliebene_Mail

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From daemon Thu Feb 14 08:18:46 2002



From: Dave Hall :      daveh-at-restechimage.com
Date: Thu, 14 Feb 2002 09:10:10 -0500
Subject: Cameras, Brightfield, DN100, DXC390, Micropublisher

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Peter:

I think your assessments have a lot to do with your requirements and
your plans for the images. If you plan on doing spatial measurements,
then you will perhaps reap added benefit from the larger resolution
options with higher pixel counts. If you are doing
color-segmentation-related measurements, then the extra color fidelity
of the 3-CCD Sony might offer the ability to better distinguish similar
colors, but at lower spatial resolution. If color fidelity is a
requirement, but so is the high pixel resolution, you could cloud the
issue even further by considering 3-CCD, mega-pixel options from Sony or
JVC. (I can provide info offline if these options are of interest.)

If you have no plans for measurement, only archival or publication, then
your assessment does fall back on the items you mentioned. I can't
speak from experience to the ease of use or software friendliness of the
Nikon or the Q-Imaging, but my guess is that both have their own
software applications that acquire and save, and both probably offer
TWAIN for use with Photoshop, etc. The Sony DXC-390 is an analog 3-CCD
camera and does not provide for its own direct acquisition of images
into the computer. This camera will have to be connected to a
framegrabber and the ease of use and software friendliness will depend
on what framegrabber board has been proposed with the camera. The image
quality from this camera will also depend on the board. The best image
quality from this camera will be the RGB output. If either the S-Video
or the NTSC outputs are used for acquisition, the image will still be
there (and the framegrabber might cost less), but it won't be the
optimal image. Software support for the framegrabber board could be the
manufacturer's application, or it could include some 3rd-party package
that might even include image analysis.

In all cases, software ease of use will depend on what functions have
been implemented into the software support. In some cases, although not
all, cameras have capabilities that can be accessed through the user
menus, or through Software Development Kit (SDK) programming calls, but
those features are not always conveniently included in the free control
application provided with the camera or framegrabber.

If I drone on much further, the relevance to the group may dwindle
quickly, if it hasn't already. I hope this info helps a little, even
though it's perhaps more conceptual, as opposed to directly related to
your three options.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045
*Please visit our website at http://www.restechimage.com

-----Original Message-----
} From: Peter Steele [mailto:STEELEP-at-allkids.org]
Sent: Wednesday, February 13, 2002 8:50 AM
To: Microscopy-at-sparc5.microscopy.com



I am assessing vastly different systems that use the Nikon DN100, the
Sony DXC390, or the Q-Imaging Micropublisher cameras for capturing
bright field microscopy (mainly frozen biopsies). I am seeking any
comments or experiences (benefits/limitations) about any of these three
cameras (or others). For example, ease of use, software friendliness,
quality, robustness, . . .

Please feel free to send responses offline, if they are not appropriate
for the list.

Thank you,

Peter O. Steele, Ph.D.
Pathology and Laboratory Medicine
All Children's Hospital
St. Petersburg, Fl


E-mail: steelep-at-allkids.org




From daemon Thu Feb 14 09:37:09 2002



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Thu, 14 Feb 2002 08:12:45 -0700
Subject: Vitreous Carbon ???

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Could somebody tell me what vitreous carbon is ?

A colleague of mine and I were taking a look at a piece of this material and we are curious to know more about it.
I know some suppliers carry vitreous carbon planchets for SEM, but we are curious to know exactly what they are .For
example, is this material carbon that has been densified more than "amorphous" carbon due to higher pressures or
perhaps the material has been "quenched" in some fashion or a combination of both ? Also, besides offering a much
smoother surface (for SEM or AFM) do these planchets have higher conductivity than regular carbon planchets ?

Thanks

Jordi


From daemon Thu Feb 14 11:04:46 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 14 Feb 2002 11:57:31 -0500
Subject: Re: Ask-A-Microscopist: osmolarity and TEM fixation

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hina-at-ohio.edu wrote:

} Email: hina-at-ohio.edu
} Name: Sarah Hina
}
} Organization: Ohio University
}
} Education: Graduate College
}
} Location: Athens, OH USA
}
} Question: I work in a lab where we are investigating hair bundles of
} the utricle (inner ear). I have a question regarding osmolarity and
} TEM fixation. We are using the De Groot fixative, which contains 3%
} Glutaraldehyde, 2% Paraformaldehyde, 1% Acrolein, and 2.5% DMSO in
} 0.8M Sodium Cacodylate-HCL buffer. The total osmolarity is 1149
} mmol/kg, considerably more hypertonic than the extracellular fluid
} (about 280 osmoles). I have read that glutaraldehyde and
} formaldehyde do not contribute significantly to the effective osmotic
} pressure of the fixative. Is this true? Does anyone know if
} acrolein and DMSO contribute to the effective pressure? We measured
} the osmolarity of our buffer alone, and it was a more reasonable 415
} mmol/kg. Is this the more important value? Also, we are fixing by
} way of vascular perfusion, which according to what I have read,
} requires a more hyperosmolar solution. I would very much appreciate
} any comments or suggestions. Thanks!
}
} ---------------------------------------------------------------------------

Read Arborgh et al. J. Ultrastruc. Res. 56:339-350, 1976. A classic work.
Basically they found that the osmolarity that mattered was the osmolarity of
the buffer, not the buffer-fixative combo. Somewhat hypertonic was better than
isotonic.
As always, the proof of the pudding is in the eating.
I am guessing that your cacodylate buffer is 0.08M, not 0.8M?

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Feb 14 13:41:42 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 14 Feb 2002 14:29:10 -0500
Subject: RE: Ask-A-Microscopist: osmolarity and TEM fixation

Contents Retrieved from Microscopy Listserver Archives
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Afternoon Sara,
This problem is quite special and most good information is derived
from studies on specific tissues. The upshot is that one is best advised to
perform one's own test starting with the information that is offered in good
primary and secondary sources.
I will recommend two secondary sources:
Stoward, P.J., Fixation in Histochemistry", Chapman and
Hall, London, 1973 (ISBN 412-12050-X)
Hayat, M.A., "Fixation for electron Microscopy", AP, NY, NY,
1981 (ISBN: 0-12-333920-0)
Hayat also has a more recent edition of his book entitled
"Principles and Techniques in Electron Microscopy" that you might also want
to consult.
For more recent contributions that might be relevant, I suggest a
search of PubMed at NCBI: http://www.ncbi.nlm.nih.gov/. I used "fixative
osmolarity inner ear" and got some interesting looking results.

Regards,

Fred Monson

Frederick C. Monson, PhD
The best research
Center for Advanced Scientific Imaging
occurs before work
West Chester University
at the bench.
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


} ----------
} From: hina-at-ohio.edu
} Sent: Wednesday, February 13, 2002 5:25 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: osmolarity and TEM fixation
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (hina-at-ohio.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
} February 13, 2002 at 11:24:14
} --------------------------------------------------------------------------
} -
}
} Email: hina-at-ohio.edu
} Name: Sarah Hina
}
} Organization: Ohio University
}
} Education: Graduate College
}
} Location: Athens, OH USA
}
} Question: I work in a lab where we are investigating hair bundles of
} the utricle (inner ear). I have a question regarding osmolarity and
} TEM fixation. We are using the De Groot fixative, which contains 3%
} Glutaraldehyde, 2% Paraformaldehyde, 1% Acrolein, and 2.5% DMSO in
} 0.8M Sodium Cacodylate-HCL buffer. The total osmolarity is 1149
} mmol/kg, considerably more hypertonic than the extracellular fluid
} (about 280 osmoles). I have read that glutaraldehyde and
} formaldehyde do not contribute significantly to the effective osmotic
} pressure of the fixative. Is this true? Does anyone know if
} acrolein and DMSO contribute to the effective pressure? We measured
} the osmolarity of our buffer alone, and it was a more reasonable 415
} mmol/kg. Is this the more important value? Also, we are fixing by
} way of vascular perfusion, which according to what I have read,
} requires a more hyperosmolar solution. I would very much appreciate
} any comments or suggestions. Thanks!
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Thu Feb 14 15:48:57 2002



From: Sarah Hina :      hina-at-ohio.edu
Date: Thu, 14 Feb 2002 16:33:04 -0500
Subject: Re: Ask-A-Microscopist: osmolarity and TEM fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Thank you very much for your suggestion--I will be sure to check out that
article. And yes, it should have read 0.08M. Thanks for your time--I
appreciate it!

Sarah

--On Thursday, February 14, 2002 11:57 AM -0500 Geoff McAuliffe
{mcauliff-at-UMDNJ.EDU} wrote:

} hina-at-ohio.edu wrote:
}
} } Email: hina-at-ohio.edu
} } Name: Sarah Hina
} }
} } Organization: Ohio University
} }
} } Education: Graduate College
} }
} } Location: Athens, OH USA
} }
} } Question: I work in a lab where we are investigating hair bundles of
} } the utricle (inner ear). I have a question regarding osmolarity and
} } TEM fixation. We are using the De Groot fixative, which contains 3%
} } Glutaraldehyde, 2% Paraformaldehyde, 1% Acrolein, and 2.5% DMSO in
} } 0.8M Sodium Cacodylate-HCL buffer. The total osmolarity is 1149
} } mmol/kg, considerably more hypertonic than the extracellular fluid
} } (about 280 osmoles). I have read that glutaraldehyde and
} } formaldehyde do not contribute significantly to the effective osmotic
} } pressure of the fixative. Is this true? Does anyone know if
} } acrolein and DMSO contribute to the effective pressure? We measured
} } the osmolarity of our buffer alone, and it was a more reasonable 415
} } mmol/kg. Is this the more important value? Also, we are fixing by
} } way of vascular perfusion, which according to what I have read,
} } requires a more hyperosmolar solution. I would very much appreciate
} } any comments or suggestions. Thanks!
} }
} } ------------------------------------------------------------------------
} } ---
}
} Read Arborgh et al. J. Ultrastruc. Res. 56:339-350, 1976. A classic work.
} Basically they found that the osmolarity that mattered was the osmolarity
} of the buffer, not the buffer-fixative combo. Somewhat hypertonic was
} better than isotonic.
} As always, the proof of the pudding is in the eating.
} I am guessing that your cacodylate buffer is 0.08M, not 0.8M?
}
} Geoff
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************
}
}






From daemon Thu Feb 14 15:48:57 2002



From: Sarah Hina :      hina-at-ohio.edu
Date: Thu, 14 Feb 2002 16:36:53 -0500
Subject: RE: Ask-A-Microscopist: osmolarity and TEM fixation

Contents Retrieved from Microscopy Listserver Archives
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Hi Fred,

Thanks so much for your suggestion--I did find a copy of the Hayat book,
which provided some good info. I think the gist of their rationale is that
the osmolarity of the buffer is more important than that of the fixtative.
In our case, the acrolein and buffer would be first to enter the tissue,
thus partially fixing it before the other fixatives could affect the
tissue. However, I will be sure to conduct a search through pubmed as
well. Thanks again for your time!

Sarah

--On Thursday, February 14, 2002 2:29 PM -0500 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} Afternoon Sara,
} This problem is quite special and most good information is derived
} from studies on specific tissues. The upshot is that one is best advised
} to perform one's own test starting with the information that is offered
} in good primary and secondary sources.
} I will recommend two secondary sources:
} Stoward, P.J., Fixation in Histochemistry", Chapman and
} Hall, London, 1973 (ISBN 412-12050-X)
} Hayat, M.A., "Fixation for electron Microscopy", AP, NY, NY,
} 1981 (ISBN: 0-12-333920-0)
} Hayat also has a more recent edition of his book entitled
} "Principles and Techniques in Electron Microscopy" that you might also
} want to consult.
} For more recent contributions that might be relevant, I suggest a
} search of PubMed at NCBI: http://www.ncbi.nlm.nih.gov/. I used "fixative
} osmolarity inner ear" and got some interesting looking results.
}
} Regards,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} The best research
} Center for Advanced Scientific Imaging
} occurs before work
} West Chester University
} at the bench.
} West Chester, Pennsylvania, USA, 19383
} 610-738-0437
} fmonson-at-wcupa.edu
}
}
} } ----------
} } From: hina-at-ohio.edu
} } Sent: Wednesday, February 13, 2002 5:25 PM
} } To: microscopy-at-sparc5.microscopy.com
} } Subject: Ask-A-Microscopist: osmolarity and TEM fixation
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (hina-at-ohio.edu) from
} } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
} } February 13, 2002 at 11:24:14
} } ------------------------------------------------------------------------
} } -- -
} }
} } Email: hina-at-ohio.edu
} } Name: Sarah Hina
} }
} } Organization: Ohio University
} }
} } Education: Graduate College
} }
} } Location: Athens, OH USA
} }
} } Question: I work in a lab where we are investigating hair bundles of
} } the utricle (inner ear). I have a question regarding osmolarity and
} } TEM fixation. We are using the De Groot fixative, which contains 3%
} } Glutaraldehyde, 2% Paraformaldehyde, 1% Acrolein, and 2.5% DMSO in
} } 0.8M Sodium Cacodylate-HCL buffer. The total osmolarity is 1149
} } mmol/kg, considerably more hypertonic than the extracellular fluid
} } (about 280 osmoles). I have read that glutaraldehyde and
} } formaldehyde do not contribute significantly to the effective osmotic
} } pressure of the fixative. Is this true? Does anyone know if
} } acrolein and DMSO contribute to the effective pressure? We measured
} } the osmolarity of our buffer alone, and it was a more reasonable 415
} } mmol/kg. Is this the more important value? Also, we are fixing by
} } way of vascular perfusion, which according to what I have read,
} } requires a more hyperosmolar solution. I would very much appreciate
} } any comments or suggestions. Thanks!
} }
} } ------------------------------------------------------------------------
} } -- -
} }
} }






From daemon Thu Feb 14 16:03:03 2002



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Thu, 14 Feb 2002 16:55:22 -0500
Subject: TEM of cellulose fibers

Contents Retrieved from Microscopy Listserver Archives
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Hello all: I am trying to find a/some references on methods for TEM of
cellulose fibers. I have found what look to be good ones but they are in
German. Can anyone direct me to something in English. Thanks in advance.

Stephen McCartney
Research Associate
2108 Hahn Hall
Materials Institute
Virginia Tech
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Thu Feb 14 16:03:04 2002



From: kbovard-at-creighton.edu
Date: Thu, 14 Feb 2002 15:56:52 -0600 (CST)
Subject: Historesin vs. Technovit

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know if there are any differences in how Technovit is used and
works compared to Historesin.

I am a clinical pathology laboratory embedding human kidneys for
diagnosis. I am used to how Historesin is processed/cuts/stains/etc.
What can I expect with Technovit? The same? In Pathology, you never
*experiment* with a patient's specimen/life...so I'm a little nervous in
having to switch from one product to another.

Any insight/experience in this issue is appreciated.

Karen Bovard
Electron Microscopy Laboratory
Department of Pathology
Creighton University/St. Joseph Hospital
Omaha, Nebraska



From daemon Thu Feb 14 17:05:27 2002



From: zaluzec-at-microscopy.com
Date: Thu, 14 Feb 2002 16:24:21 -0600
Subject: Administrivia: Haengengebliebene is now blocked

Contents Retrieved from Microscopy Listserver Archives
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Colleagues...

Sorry, but out here in Oz I'm a half day out of sync.
Haengengebliebene is now blocked and once the queues clear
it should not get through any longer.

Nestor
Your Friendly Neighborhood SysOp


From daemon Thu Feb 14 17:05:27 2002



From: mp0017-at-unt.edu ()
Date: Thu, 14 Feb 2002 16:17:11 -0600
Subject: Ask-A-Microscopist:HREM Question

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mp0017-at-unt.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
February 14, 2002 at 13:45:05
---------------------------------------------------------------------------

Email: mp0017-at-unt.edu
Name: M. Pritchett

Organization: University of North Texas

Education: Graduate College

Location: Denton, TX

Question: We are trying to get atomic resolution and find
after spot welding the sample very well, it only
seems to hold through two annealing cycles.
(We anneal to 650K for 30 minutes to get the
surface smooth.) It seems the repeat annealing
loosens the sample because then we get lots of
noise.

Is there some kind of adhsive that can be used to
keep a Cu(1 1 1) sample from moving? Or is there
another soution to our problem?



---------------------------------------------------------------------------


From daemon Thu Feb 14 17:05:27 2002



From: pdrum-at-island.net ()
Date: Thu, 14 Feb 2002 16:16:01 -0600
Subject: Ask-A-Microscopist: microscope maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (pdrum-at-island.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
February 14, 2002 at 10:38:09
---------------------------------------------------------------------------

Email: pdrum-at-island.net
Name: Peter Drummond, Ph.D.

Organization: North Island College

Education: Undergraduate College

Location: Port Alberni, British Columbia

Question: How in most junior colleges, are the inventory of
microscopes maintained in working order? And Would you know if they
are any microscope maintenance programs available in the next... say
6 months? Thanks.

---------------------------------------------------------------------------


From daemon Thu Feb 14 18:41:20 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 14 Feb 2002 16:29:44 -0800
Subject: Mica pores and gel filling

Contents Retrieved from Microscopy Listserver Archives
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Greetings:

Please put your thinking caps on and help me with advice for a researcher
here with an interesting problem.

The researcher in question has made 100 nm pores in mica which he fills
with polyacrylamide gel. He would like to visualize the pores and see if
they are filled with gel, or if not, what the proportion of gel to space is
in the pore.

He vetoed our SEM because the gel will shrink too much for him to see what
is going on. I suggested an ESEM but don't know if that would really work.

I thought of doing some kind of fluorescent labels and then maybe something
like a confocal but he says he hasn't found a dye that will work for that
approach.

He can make the pores larger, up to a micrometer or so, but 100 nm is his
nominal best size for other reasons. If you have any ideas or practical
experience with this kind of problem, please let me know and I will forward
your words of wisdom to him.


Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Feb 15 04:31:24 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Fri, 15 Feb 2002 11:58:23 -0500
Subject: Historesin vs. Technovit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jon

AFM in Tapping mode with phase imaging may be able to visualise this. The
phase imaging will be able to tell between the mica and the polyacrylamide.
This may, however be practically difficult as the acrylamide may cause
problems with the tip.


Giles
***************************************************************************

Dr. Giles H.W. Sanders

DeltaDOT Ltd.
PFSG group
ACE Building
Department of Bioengineering
Imperial College
London
SW7 2AY

0207-594-5174
0794 - 1312335


www.deltadot.com



Information on scanning probe & microfluidics : www.achem.ic.ac.uk/gsanders
****************************************************************************
*******
****************************************************************
----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, February 15, 2002 12:29 AM


Karen,

I'm certain you will find the Technovit H7100 kit to be identical to Leica
Historesin in all facets. However, if someone on the list has a different
opinion I would be very eager to hear it. My knowledge comes not so much
from use but from my knowledge of the source for and contents of both kits.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"

Disclaimer:
Energy Beam Sciences, Inc. is the North American distributor for Heraeus
Kulzer "Technovit" Products.



-----Original Message-----
} From: "kbovard-at-creighton.edu"-at-sparc5.microscopy.com
[mailto:"kbovard-at-creighton.edu"-at-sparc5.microscopy.com]
Sent: Thursday, February 14, 2002 4:57 PM
To: Michiel De Mol
Cc: Microscopy Listserver


Does anyone know if there are any differences in how Technovit is used and
works compared to Historesin.

I am a clinical pathology laboratory embedding human kidneys for
diagnosis. I am used to how Historesin is processed/cuts/stains/etc.
What can I expect with Technovit? The same? In Pathology, you never
*experiment* with a patient's specimen/life...so I'm a little nervous in
having to switch from one product to another.

Any insight/experience in this issue is appreciated.

Karen Bovard
Electron Microscopy Laboratory
Department of Pathology
Creighton University/St. Joseph Hospital
Omaha, Nebraska





From daemon Fri Feb 15 12:47:21 2002



From: Delilah Wood :      wood-at-pw.usda.gov
Date: Fri, 15 Feb 2002 10:37:03 -0800
Subject: Re: Historesin vs. Technovit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've used both (Technovit 7100 and Historesin; also JB-4 and have made my
GMA from the components). I use Technovit 7100 routinely and have found
that it is very similar (if not identical) to Historesin. I use the
standard recipe that comes with the package. You can purchase Technovit
7100 from Energy Beam Sciences. I just bought some and they have it in stock.

De Wood

At 03:56 PM 2/14/2002 -0600, kbovard-at-creighton.edu"-at-sparc5.microscopy.com
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Feb 15 12:56:30 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Fri, 15 Feb 2002 13:50:53 -0500
Subject: Thanks, and another question

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,

Thanks to all who repsonded to my inquiry about my densi-timer. There were a lot of varied replies and a lot of folks still like to use film over digital.

Now for the next question....

I have 2 Zeiss EM-10's they both have attached to them a monitor that counts down the number of negatives left in the camera, tells the filament hours used and also tells a pre-vac pressure. One of them has now gone belly up when it comes to counting down the negatives left. Now I know that we could just do it the old fashioned way and count down using our hands and feet, but no one in the lab has 23 digits.

The company that made it was FBN Electronics, the number in New Jersey now belongs to a family. The model # is 10-4.

If any of you good buddies know anything about how to contact this company or knows if they just don't exist anymore, please let me know.


That's a big 10-4 good buddy,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Fri Feb 15 13:13:05 2002



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Fri, 15 Feb 2002 11:06:33 -0800
Subject: Mica pores and gel filling

Contents Retrieved from Microscopy Listserver Archives
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Hi Jonathan,
I think your suggestion of using an ESEM is spot on. Do you have
access to one? It would provide you an opportunity to examine the material
without a coating layer, and with minimal impact on the gel. Possibly a BSE
detector might provide some enhanced contrast, but you'd have to test that.
Good luck.

-Brad

-----Original Message-----
} From: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Thursday, February 14, 2002 4:30 PM
To: Microscopy-at-sparc5.microscopy.com


Greetings:

Please put your thinking caps on and help me with advice for a researcher
here with an interesting problem.

The researcher in question has made 100 nm pores in mica which he fills
with polyacrylamide gel. He would like to visualize the pores and see if
they are filled with gel, or if not, what the proportion of gel to space is
in the pore.

He vetoed our SEM because the gel will shrink too much for him to see what
is going on. I suggested an ESEM but don't know if that would really work.

I thought of doing some kind of fluorescent labels and then maybe something
like a confocal but he says he hasn't found a dye that will work for that
approach.

He can make the pores larger, up to a micrometer or so, but 100 nm is his
nominal best size for other reasons. If you have any ideas or practical
experience with this kind of problem, please let me know and I will forward
your words of wisdom to him.


Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Feb 15 22:12:41 2002



From: EBMet-at-aol.com
Date: Fri, 15 Feb 2002 22:58:39 EST
Subject: Metallographic Prep for Bismuth Telluride Thermoelectric Devices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List Serverites:

Does anyone know of metallographic preparation techniques to produce
cross-section specimens of bismuth-telluride (Bi2Te3) materials for
thermolectric device applications. These are either single crystal or powder
metallurgy materials. I wish to observe the cross-sections via light
microscopy and SEM. Thanks in advance.

Elliot Brown
ebmet-at-aol.com


From daemon Sat Feb 16 08:55:12 2002



From: DrJohnRuss-at-aol.com
Date: Sat, 16 Feb 2002 09:43:36 EST
Subject: Downloadable Photoshop convolution plug-in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Photoshop is a widely used program for the acquisition, processing,
annotation and printing of scientific images, and is frequently discussed on
this list. It includes a custom convolution filter that allows users to enter
a 5x5 array of integer weights that are multiplied by pixel values to produce
smoothing, derivatives, high pass filters, and other useful effects. The
interactive preview gives immediate feedback on the results, which is a great
assist to learning about convolution kernels in general, and the operation
can also be scripted in Actions for one-button application to images or for
batch processing.

Unfortunately, the Photoshop “Custom†filter has a few significant
limitations:
1. only works with 8 bit per channel grey and RGB images
2. applies the kernels separately to the RGB channels
3. is limited to a 5x5 array of integer coefficients, with integer scaling
4. saves and loads files in a special binary format

We’ve written and are offering for free download and use a very much enhanced
version of this “custom†filter. Like the Photoshop one, it has an
interactive preview, and is recordable in Actions. In addition, the plug-in:
1. supports both 8 and 16 bit per channel grey and RGB images
2. works on the intensity channel leaving hue and saturation unchanged
3. accepts up to a 7x7 array of floating point (real number) values, for much
greater precision
4. can scale the results with a floating point number, or automatically for
maximum unclipped contrast
5. reads Photoshop format files AND ALSO plain ascii text files
6. works in Photoshop and all compatible programs on both Mac and Windows
computers

Encouraged by the recent response by readers of this list to our offering a
free plug-in to draw magnification bars on micrographs, we are offering this
plug-in for free download and use. It is one of nearly 200 plug-ins in the
widely used Fovea Pro package, but can be used without installing or owning
that package.

The plug-in, along with instructions and some example filter files, can be
downloaded as a .sit file for Macintosh or a .zip file for Windows from
{http://www.reindeergraphics.com/free.html#custom} . While you are there,
please take a look at the full range of Fovea Pro capabilities, which include
comprehensive tools for image processing and analysis. Also, check out some
of the other free downloads that are available.


From daemon Sat Feb 16 15:02:46 2002



From: DrJohnRuss-at-aol.com
Date: Sat, 16 Feb 2002 15:52:39 EST
Subject: Image Processing and Measurement Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


During the last week, several people have emailed me that they have tried to
access the site with information on our short course but were unable to
because of a server problem, which is now (apparently, hopefully, finally)
fixed. Thanks for your patience. The May course is filling up but there are
still places available. If you are interested in learning practical methods
for the processing and measurement of images, check it out and sign up now.

The three-day intensive hands-on workshop on Image Processing and Measurement
presented by John Russ (author of "The Image Processing Handbook" and
"Practical Stereology") through the North Carolina State University
Department of Continuing and Professional Education is now in its 20th year.
The course dates for 2002 are May 8 - 10 in Raleigh, NC; June 10-12 at the
Danish Technological Institute in Taastrup, Denmark (near Copenhagen); and
November 6-8, 2002, in Raleigh. This course has generated highly favorable
reviews from the thousands of previous students. The primary focus is on
images from various types of microscopy, with practical guidance in
correcting imaging defects, enhancing the images for presentation and
measurement, and performing stereological meaningful measurements on them.
Textbooks and computer software are provided to attendees. Lab sessions with
an opportunity to bring your own images makes this course immediately useful
and highly productive.

For full information on the course, including outlines, faculty information,
a downloadable brochure, and on-line registration, go to

http://members.aol.com/ipcourse

Class size is limited to maintain a high ratio of instructors to students, so
make your reservation now. You may also contact Cindy Allen at NCSU
Continuing Education, at 919-515-8171



From daemon Sat Feb 16 19:47:34 2002



From: Joanne Whallon :      whallon-at-pilot.msu.edu
Date: Sat, 16 Feb 2002 20:38:42 -0500
Subject: Safety and family plan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello, List Folks:

The safety and family planning problem occurs in light microscopy also.

Immersion oil may contain dibutyl phthalate (DBT), which can harm a
developing fetus and also cause temporary male sterility.

Even though DBT is ubiquitous (in rocket fuel, paint thinner, fingernail
polish, et al.,) and even though, so far as I know, there have been no
studies on its absorption through the skin, its very, very, VERY high
concentrations in some oils (hundreds or even thousands of times higher
than OSHA standards for its presence in food, water or air), and the fact
that most of our users are in their child-bearing years, seem to merit some
concern. We now use a DBT-free oil, and ask our users to wash their hands
before leaving the lab. (After all, before DBT, immersion oil contained
PCBs!)



Joanne H. Whallon, Ph.D., Professor
Department of Crop and Soil Sciences and
Center for Advanced Microscopy
Michigan State University
East Lansing, MI 48824-1325

Phone 517/353-0837 or 517/432-2328
Fax: 517/353-3955
E-mail: whallon-at-pilot.msu.edu



From daemon Sat Feb 16 21:44:06 2002



From: Toby Knight :      tknight-at-waite.adelaide.edu.au
Date: Sun, 17 Feb 2002 14:04:35 +1030 (CST)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe



Toby G. Knight


From daemon Sat Feb 16 22:06:39 2002



From: xf200-at-attbi.com
Date: Sat, 16 Feb 2002 22:59:15 -0500
Subject: RE: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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It is not fair that the student does not have any chance to respond.

If I were that student, here is my respond:

You may think that giving me straight answer seems too easy on me. But
on my side,
it is not that simple to use your answer. If I ask my professor
directly to get an answer,
as long as I can repeat his/her words like 3 years old, I can get a A,
even if he is WRONG.

But asking you is completely different. You could be WRONG too! If I
took your
wrong answer straight to my professor, I will fail. No matter how I
explain this is from
some "expert", it is not going to help.

Oh, how could YOU be wrong? Isn't that very insulting since you are the
expert?
If you felt that way, I am sorry. I did not mean to insult any one.
However, how about
post your answer to these "elementary" questions on this list server? Do
you think
everyone on this list will agree with you?

Here are the re-post of Jenny Wang's original question:
1. What are the advantages and disadvantages in using
electrons for microscopy rather than light?
2. Does the wavelength of the electrons have anything
to do with the spatial resolution that the microscope
produces in the final picture?
3. What is temporal resolution and how is it produced
in the electron microscope?

Thank you very much in deed.



From daemon Sun Feb 17 11:26:05 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 17 Feb 2002 12:12:20 -0500
Subject: Re: Mica pores and gel filling

Contents Retrieved from Microscopy Listserver Archives
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"Johnson, Bradley R" {Bradley.Johnson-at-pnl.gov}
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From: Debby Sherman :      dsherman-at-purdue.edu
Date: 17 Feb 2002 12:12:20 -0500
Subject: Re: Mica pores and gel filling

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Cryo-SEM would work well for this problem. The fast freezing would prevent shrinkage of the gel. In addition, if there is a thin surface layer of water it can be sublimated off to reveal the underlying surface. Since cryo-microscopes usually incorporate a coater, the sample could be coated and then viewed while frozen. If you are lucky enough to find a FEG-SEM with cryo than you may be able to work at low kV without coating. The sample could be removed, thawed, and used for another purpose if desired (with the understanding that the freeze-thaw could change the properties of the sample). We have done a lot of work with cryo-SEM and hydrated gels and it works great.
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907


On Friday, February 15, 2002 2:06 PM, Johnson, Bradley R {Bradley.Johnson-at-pnl.gov} wrote:
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From daemon Sun Feb 17 11:26:46 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Sun, 17 Feb 2002 12:30:29 -0500
Subject: Re: Safety and family plan

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For most people, exposure to dibutyl phthalate in its role as a vinyl
plasiticizer is perhaps more relevant than any amount that might be
present in rocket fuel. (Babies, as well, seem more likely to chew on
toys than the on the occasional chunk of propellant left lying around.)

If my memory is correct, phthalate plasticizer contents in soft vinyl
have sometimes run up to about 20% by weight. Such plastics have been
used in many large household items and probably account for a much more
important route of exposure than immersion oils - even for sloppy
microscopists. Vinyl's tendency to soften and cause printing ink and
photocopy toner to transfer to adjacent surfaces is due to plasticizer
migration. The loss of plasiticizer due to its volatilization and
"sweating" gives rise to shrinkage and cracking in such plastics. In
years past, who hasn't gotten into a hot car and smelled the bland but
noticeable odor?

On the other hand, in 25 years of microscopy, I can't recall getting
more than a milligram or two of immersion oil (PCB free!) on my skin. I
guess that depends on one's technique.

While the jury may still be out on the full range of dibutyl phthalate
effects, one who is concerned would want to deal with the major routes
of exposure first. Perhaps you could expand on your other steps for the
list.

What is the composition of your phthalate-free immersion oil?

Your last comment seems to invite the conclusion that immersion oils are
inherently suspect, or that you believe phthalates will be found to pose
risks similar to PCBs. Is that the case?

John Twilley

Joanne Whallon wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, List Folks:
}
} The safety and family planning problem occurs in light microscopy also.
}
} Immersion oil may contain dibutyl phthalate (DBT), which can harm a
} developing fetus and also cause temporary male sterility.
}
} Even though DBT is ubiquitous (in rocket fuel, paint thinner, fingernail
} polish, et al.,) and even though, so far as I know, there have been no
} studies on its absorption through the skin, its very, very, VERY high
} concentrations in some oils (hundreds or even thousands of times higher
} than OSHA standards for its presence in food, water or air), and the fact
} that most of our users are in their child-bearing years, seem to merit some
} concern. We now use a DBT-free oil, and ask our users to wash their hands
} before leaving the lab. (After all, before DBT, immersion oil contained
} PCBs!)
}
}
}
} Joanne H. Whallon, Ph.D., Professor
} Department of Crop and Soil Sciences and
} Center for Advanced Microscopy
} Michigan State University
} East Lansing, MI 48824-1325
}
} Phone 517/353-0837 or 517/432-2328
} Fax: 517/353-3955
} E-mail: whallon-at-pilot.msu.edu
}
}
}
}



From daemon Sun Feb 17 13:25:15 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Sun, 17 Feb 2002 13:15:47 -0600
Subject: Zeiss Axiophot lamp problem

Contents Retrieved from Microscopy Listserver Archives
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My Zeiss Axiophot microscope has a recurring problem and I am
wondering if any other users have the same problem and if they have
come up with a permanent solution. The bright field lamp sits in a
ceramic base and with time, the heat apparently cracks the base. As
this is slowly happening, the lamp is no longer sitting firmly in its
socket so the light fluctuates constantly. You can see it
"breathing" as you watch the live image captured with a digital
camera. I have replaced the ceramic socket at least 3 times and need
to once again. Zeiss has not offered any solution other than to sell
me a new socket each time. Any suggestions would be greatly
appreciated.

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Sun Feb 17 16:15:54 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sun, 17 Feb 2002 15:10:13 -0700
Subject: student help -- poll results

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers,

I have waited for a week now and there don't seem to be any new responses to
the "poll". Thanks to anybody who answered. As promised, I took a look at
the numbers and here are the results. I am not going to interpret the
numbers, you can do that for yourself.

The choices were:

} 1) Students should work through their assignments alone. Let's not support
} laziness at all.
} 2) We should help them help themselves by pointing them to general
} microscopy books.
} 3) We should take their questions and point them to specific microscopy
} books dealing with their problems
} 4) We should encourage them to contact us offline so we can test their
} sincerity and then either help them or not. This should be posted on the
} server to avoid redundancy.
} 5) We should help them with their questions by trying to answer them in a
} general way
} 6) We should go all out and answer their questions as best as we can.

In total I counted 51 responses both to the list server and my email. I took
out the double answers that were sent to both the list server and to me. Of
the 51 responses, 25 were from educational facilities, 18 from commercial
facilities, and 8 were from other sources (government, AOL, foreign). These
classification was done on the email extension. A number of people selected
more than one answer. In these cases I simply split up their single vote
into partial votes. for example, if someone said "2,3, and 6", each of the
choices got 0.33 votes. Finally, a couple of people thought, the selection
of choices was too narrow and made other suggestions or withheld, which I
put into number "7".

My intention was at no time to make this an exhaustive scientific article. I
kept the choices simple and I only want to present the numbers in a simple
way here. If you want to discuss the numbers, please do so, I will draw no
conclusions from the numbers presented here.

Overall the results are as follows:

1 2 3 4 5 6 7 {-
selection
1.2 9.53 23.03 4.03 4.7 6.5 2 {- number
of votes

for .com
1 2 3 4 5 6 7 {-
selection
0.2 2.7 9.2 2.2 1.7 2 0 {-
number of votes

for .edu
1 2 3 4 5 6 7 {-
selection
1 6.83 5.83 1.83 3 4.5 2 {- number
of votes

for other
1 2 3 4 5 6 7 {-
selection
0 0 8 0 0 0 0 {-
number of votes


Unfortunately I can't send the Excel datasheet I used to tally the votes and
show the numbers to the list server. If you are interested, drop me a line
and I will send it to you.

mike

} } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Ave #300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================




From daemon Sun Feb 17 16:47:33 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 17 Feb 2002 14:44:43 -0800
Subject: Re: Zeiss Axiophot lamp problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had similar, but not identical problems, with my Axioskop
and Axioplan lamp houses. Generally, they were quite good.
If the lamps are run at full voltage (12VAC), they will get
really hot and not last all that long. Also, as was recently
discussed, line voltage is not constant. The Zeiss lamp
controllers are simple triac controls which are not regulated.
So, if your line voltage fluctuates, so will your lamp. that is
the way it is.

I ran my halogen lamps at 10VDC using a Lambda regulated
DC power supply. I also put a 12VDC 60mm fan next to the
lamp housing. It turned out that just lowering the voltage made
a huge difference. No fluctuation and long lamp life. The
Axioplan stand seemed to have a regulated lamp source and
would easily accommodate a 10V setting. I use HAL 100W
12V halogen bulbs (Osram). The same principle applies to
any other wattage rating bulb. I solved this voltage fluctuation
problem by converting all UPS units to dual conversion. My
line logs show minimum line voltages of 103VAC to peak voltages
of 118VAC. The UPS puts out a constant 120VAC....+- 3%.
Nailed.... Yes, the units are a bit more expensive than passive
UPS, but I don't have to deal with the hassle any longer. Even
my unregulated Fostec ACE units work perfectly now and I
don't have to buy DCR units at twice the price. I think this is
a win situation.

Try using a lower voltage setting and see if that works for you.
If not, put a cooling fan next to a vent port on the lamp house.
Put it close to, but not on, the house. Otherwise, you will get
vibration interference at high mag.

Contact me off-line if you need further info.

gary g.


At 11:15 AM 2/17/2002, you wrote:

} My Zeiss Axiophot microscope has a recurring problem and I am wondering if
} any other users have the same problem and if they have come up with a
} permanent solution. The bright field lamp sits in a ceramic base and with
} time, the heat apparently cracks the base. As this is slowly happening,
} the lamp is no longer sitting firmly in its socket so the light fluctuates
} constantly. You can see it "breathing" as you watch the live image
} captured with a digital camera. I have replaced the ceramic socket at
} least 3 times and need to once again. Zeiss has not offered any solution
} other than to sell me a new socket each time. Any suggestions would be
} greatly appreciated.
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility



From daemon Sun Feb 17 20:48:43 2002



From: Michael Dingley :      michaeld-at-austmus.gov.au
Date: Mon, 18 Feb 2002 13:39:52 +1100
Subject: Portable Microscope Catalogue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have just produced a Catalogue of Portable Microscopes on CD-ROM. It
lists approximately 280 instruments with 99% of them illustrated. It is
ideal for microscope collectors and scientific instrument museums etc.
For more information go to http://www.pnc.com.au/~dingley

Mike Dingley
(I have a personal, financial and commercial interest in the CD).


This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, you must not copy, take action on, or disclose any details of this information to any other person or organisation. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Australian Museum.




From daemon Mon Feb 18 07:36:47 2002



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Mon, 18 Feb 2002 08:26:03 -0500 (EST)
Subject: TEM of B-galactosidase

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anyone know of a way to detect beta-galactosidase by TEM? The B-gal
is expressed in transgenic mice. We would like to see it in the
fibroblasts of nerve tissue. So far our literature search has yielded
information at the light microscopy level only. Any ideas out there?

Thanks!

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu




From daemon Mon Feb 18 08:00:30 2002



From: AMCGroup2-at-aol.com
Date: Mon, 18 Feb 2002 07:52:05 -0600
Subject: Looking for Contract SEM/TEM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear All,

We currently have a need for ongoing EM-based (SEM and/or TEM) imaging and
chemical analysis on a hourly or contract basis and full-service or
instrument-only arrangements.

AMC Group is a consultancy based in New Mexico
involved in physical and chemical characterization of advanced optoelectronic
materials and devices. I would
appreciate receiving responses from the intersested parties in US at both
commercial labs and university labs off-line.

James Glossinger
AMC Group, Inc.
amcgroup2-at-aol.com


From daemon Mon Feb 18 13:25:08 2002



From: Gang Ning :      gning-at-mcw.edu
Date: Mon, 18 Feb 2002 13:08:55 -0600
Subject: Re: TEM of B-galactosidase

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
At least two papers did B-gal enzyme cytochemistry at EM level. See Sekerkova

G. et al J Histochem Cytochem 45:1147-55, 1997 and Weis J. et al. JCB
113:1385-97, 1991. The method works well.

G. Ning
Electron Microscopy Facility
Medical College of Wisconsin

Dorothy Roak Sorenson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Does anyone know of a way to detect beta-galactosidase by TEM? The B-gal
} is expressed in transgenic mice. We would like to see it in the
} fibroblasts of nerve tissue. So far our literature search has yielded
} information at the light microscopy level only. Any ideas out there?
}
} Thanks!
}
} Dotty
}
} Dotty Sorenson
} Microscopy and Image Analysis Laboratory
} Department of Cell and Developmental Biology
} University of Michigan Medical School
} Ann Arbor, Michigan
} (734)763-1170
} FAX (734)763-1166
} dsoren-at-umich.edu



From daemon Mon Feb 18 14:06:24 2002



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Mon, 18 Feb 2002 14:50:38 -0500
Subject: Food Structure & Functionality Forum Symposium 2002 - Final

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Food Structure & Functionality Forum Symposium 2002
May 5 to 8, 2002, Palais des Congrès de Montréal, Montréal, Quebec, Canada

Monday, May 6th-Morning
Opening of symposium

Presentation of Division Achievement Award to Dr. R. Gary Fulcher, University of Minnesota

Plenary address by R. Gary Fulcher: Title to be announced

Dairy Applications Session Chairs: Mark Auty, Dairy Products Research Centre, TEAGASC,
Ireland (mauty-at-moorepark.teagasc.ie ) and Harjinder Singh, Massey University, NZ
(H.Singh-at-massey.ac.nz)

Manufacturing Yoghurt Structures with a Predicted Consumer Preference. M Langton and
A. Astrom, SIK, Sweden

Some Structure-Function Relationships in Milk Gels. J. Lucey, University of Wisconson-Madison, USA

Understanding the Functionality of Whey Proteins as Microencapsulating Agents. M. Rosenberg and M.H. Wang, University of California - Davis, USA

Carry-over Effects of Processing Parameters as Determined During Sweetened Condensed Milk Manufacture and Application. C. Attapattu, University of Wisconsin, USA and Y. Kakuda, University of Guelph, Canada.

Modifying Dairy Protein Functionality Through Extrusion Processing. C. I. Onwulata and R. P. Konstance, USDA-ARS-ERRC, USA

Some Observations of a Microscopist, Author, and Reviewer on Structural Studies of
Milk Products. M. Kalab, Agriculture and Agri-Food Canada, Canada (Keynote Speaker)

Monday, May 6th - Afternoon (1:00-4:00PM Note Early Start)
Colloidal and Interfacial Sciences Session.
Chairs: Marcel Paques (Paques-at-nizo.nl ) and David Pechak (Dpechak-at-kraft.com)

rotein Polysaccharide Interactions. C.G. De Kruif, NIZO Food Research, Netherlands (Keynote Speaker

Influence of Protein Adsorption Conditions upon Oil-in-Water Emulsion Stability: A Comparison of b-Lactoglobulin to a Whey Protein Concentrate. B. Campbell1, I. Ivanon2, N. Denkov2, S. Tcholakova2, 1Kraft Foods,USA; 2University of Sofia, Bulgaria

Microstructure of Heat Processed Whey Protein Food Emulsion and Growth of Shear-Induced Cracks During Cooling. R. Ofstad1, V. Hoest1, O. Langsrud1, G. Enersen1, T.E. Nyvold2, E.P. Willers3, B. Nordvi4, B. Egelandsdal1,5, 1 Norwegian Food Research Institute-MATFORSK, Norway; 2Stabburet, Norway; 3Procordia Foods, Sweden; 4TINE Norwegian Dairies, Norway; 5Institute of Food Research, Agricultural University of Norway, Norway

Fatty Acid Salts-Induced Gels of Food Proteins: Their Rheological Properties and
Structural Changes of the Proteins During Gelation. N. Yuno-Ohta, Nihon University, Japan

Wheat Gluten Proteins. A.S. Tatham, IACR Long Ashton Research Station, United Kingdom

nterfacial Composition and Stability of Oil in Water Emulsion formed with Mixtures of
Milk Proteins and Polysaccharides. H. Singh and Y. Hemar, Institute of Food, Nutrition and
Human Health, Massey University, NZ

Dedicated Poster Session

Division Board Meeting

Tuesday, May 7th - Morning (9AM-12PM)
Agricultural Applications of Microscopy and Imaging Session/ joint with Feed
Microscopy Division. Topic: Food Contamination
contacts: Mark Auty, Dairy Products Research Centre, TEAGASC
(mauty-at-moorepark.teagasc.ie ) and Kim Koch, North Dakota State University (kkoch-at-ndsuext.nodak.edu) (Feed Microscopy Division)

TBA. J. Shane, McCrone Institute.

How to approach contaminant identification. M. Auty, Dairy Products Research Centre,
Ireland

Identification of plant material. D.F. Wood, USDA, USA

310 Species identification of Animal Hair by Using Atomic Force Microscopy. C.W.
Cruywagen, University of Stellenbosch, South Africa.

401 Detection and Differentiation of APRALAN, PAYLEAN, PULMOTIL and TYLAN in
Animal Feeds using microscopy. P. Klink. Elanco Animal Health, a Division of Eli Lilly and
Company, USA.

Quantitation of Total Fat and Fat Quality in Cheese and Dairy Products Using Membrane
Separation Technology. V.C. Gordon, Safety Associates, Inc., USA

--------------------------------------------------------------------------------

Division Luncheon and round table (expert) discussion (12-2PM). Topic: Structure - functionality
relationships in materials containing fats and oils: Drafting a Roadmap for the Future.

Specifics: Microstructure, Crystal Structure and Molecular Structure: Imaging, Quantification,
and Relationships to Macroscopic Functionality - what is the state of the art, which relationships
continue to baffle us, what are the promising new technologies/characterization
methods/modeling activities?

Discussion leaders: M. Auty, M. Paques ( Food Structure and Functionality Forum) and
S. Narine, N. Widlak (Edible Applications Division)
*--------------------------------------------------------------------------------

Tuesday, May 7th - Afternoon (
Microbiology and Food Session Chairpersons: Judy Arnold and Ida Yates, USDA, ARS,
Russell Research Center, USA



Prevention of Bacterial Fouling on Food Equipment Surfaces. J.W. Arnold, USDA, ARS, Russell Research Center, USA (

TBA. S. Pao, Virginia State University, USA

Yogurt microstructure and texture: The role of exocellular polysaccharides produced by lactic acid bacteria. J. F. Frank and A.N. Hassan. University of Georgia, USA

Probiotics and Their Use in Food Animal Production. R. Droleskey, USDA, ARS, SPARC, USA

Ultra-structural analysis of the monospecies biofilms formed by Listeria monocytogenes. M.L. Kalmokoff and J.W. Austin; BMH, Health Canada, Canada

The Effect of High Pressure Sterilization on Listeria Inoculated Seafood. K.R.S. Schneider and M.V.W. Wood, University of Florida, USA

Food Microstructure Investigations by Atomic Force Microscopy. J. Thornton, Digital Instruments/Veeco Metrology Group, USA (20 min)

Controlling Growth of the Toxigenic Fungus, Fusarium Verticillioides. I. Yates and J. Arnold, Russell Research Center, ARS, USDA, USA

Division Members Meeting (immediately following the afternoon session)

Wednesday, May 8th- Morning (8AM-12PM)
Ingredients and Food Processing Session- Chairpersons: Diana Kittleson, General Mills
Technology East, USA; and Bernhard Tauscher, Federal Research Center for Nutrition,
Germany

Applications of Food Material Science. D.W. Stanley, University of Guelph, Canada (Keynote Speaker )

Non-Invasive Quality Determination of Fruit and Vegetables: Application of a Multi-
Wavelength NIR-Diode Laser Array. B. Tauscher, P. Butz, Federal Research Center for Nutrition,
Germany

Microstructure Characterization of Polysaccharide Based Films. G. Frias2, M.A. Garcia1, M. Martino1,2. 1CIDCA,
UNLP, CONICET, Argentina, 2 Faculty of Engineering, UNLP, Argentina

Characterisation of the Application of Novel Oil Structurants. E. Floeter, F. Gandolfo, and
W. Hogervorst, Unilever Research Vlaardingen, The Netherlands

Fat Bloom Formation and Characterization in Milk Chocolate Observed by Atomic Force Microscopy. S.M.Hodge, D. Rousseau, Ryerson University, Canada

High Pressure Processing. E. Ting and E. Raghubeer, Flow International, USA

Microstructure of Rice Starch Isolates. D.F. Wood1, A.M. Ibanez_Carranza2, and C.F.
Shoemaker2, 1USDA, ARS, WRRC, USA; 2University of California, USA

Structural Properties of Multiphase Potato Products. M. Lamberti,F. Escher, B. Conde-Petit, ETH, Switzerland

Effect of Processing on Microstructure of Oat Starch. M. Salmenkallio-Marttila , K. Autio, VTT Biotechnology, Finland

Novel Techniques for the Production of Functional, Less Beany-Flavored Soy Products J. I. Boye, Agriculture and Agri-Food Canada, Canada. (

Wednesday, May 8th - Afternoon (2-5PM)
New Methods and Techniques for Food Structure and Functionality Analysis Session
Chairpersons: Kathy Groves, Leatherhead Food Research Association, England; and Maud
Langton, SIK, Sweden

Quantifying Microstructures through Image Analysis. G.M.P. van Kempen1, M. van
Ginkel2, C.L. Luengo Hendriks2, L.J. van Vliet2, and S. Singleton3, 1Unilever Research
Vlaardingen, Netherlands; 2Delft University of Technology, The Netherlands; 3Unilever
Research Colworth House, Great Britain (Keynote Speaker )

Changes in plant tissue after pulsed electric field treatment. M. Fincan, P. Dejmek
Dept. of Food Engineering, Lund University, Sweden

Cryo-TEM of Biopolymers in Comparision with Other TEM-techniques. M. Langton, A.
Altskar, and A.-M Hermansson, SIK, Sweden

Freeze-substitution and low temperature embedding of dairy products for electron
microscopy.
A.K. Smith and H.D. Goff, Department of Food Science, University of Guelph, Canada

MicroRheology: preliminary results of structural behaviour of foods under deformation.
M. Paques, Y. Nicolas, Wageningen Centre for Food Sciences/Unilever Vlaardingen, The
Netherlands.

Recent Advances in our Understanding of the Relationship Between Crystallization
Behavior, Microstructure and Rheological Properties of Fat Crystal Networks. A.
Marangoni, University of Guelph, Canada

CLOSURE OF SYMPOSIUM

Posters
Spectroscopic Prediction of Rheological Properties in Grains. F. Meadows and F. Barton
USDA, ARS, QARU, USA

Staining Techniques for Detection of Components in Fish Muscle. K. Hanneson Eggen and
G. Enersen, Matforsk, Norway

Effect of Emulsifiers and Processing Conditions on Microstructure of Milk Fat/Sunflower
Oil Blends. S. Martini1, M. Cerdeira1, C. Puppo1, R.W. Hartel2, and M.L. Herrera1,3, 1CIDCA,
UNLP, CONICET, Argentina; 1University of Wisconsin-Madison, USA; 3University of Buenos
Aires, Argentina

Exchange in Semi-Solid Triglyceride systems measured by NMR Spectroscopy: Effect of
Partial Glycerides on Exchange Rates. P. Smith1, N. Haghshenas1, I. Furo2, and B.
Bergenstahl3, 1YKI Institute for Surface Chemistry, Sweden; 2Royal Institute, Sweden; 3Lund University, Sweden.

Utilizing Polarized Light Microscopy to Characterize the Effects of Tween60 on the
Physical Properties of a Model Plastic Fat System. J.W. Litwinenko and A.G. Marangoni,
University of Guelph, Canada.

Relationships between Microstructure and Rheological Properties of Model Lipid
Systems. B. Liang, Y. Shi, and R.W., Hartel Univ. of Wisconsin-Madison, USA

Texturization of Water-in-oil-Based Spreads. Y. Shi, B. Liang, and R.W. Hartel, University of
Wisconsin-Madison, USA

Effect of Temperature and Shear Rate on Fat Crystallization Kinetics. Ph Rousset, C. Bertoli, H. Dux, Nestle Research Center, Switzerland

Formation and Physical Properties of ?-Fat Gel. I: Macroscopic and Microscopic
Observations. K. Higaki1, Y. Sasakura1, I. Hachiya1, and K. Sato2, 1Meiji Seika Kaisha Ltd.,
Japan; 2Faculty of Applied Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. Ii: In situ Observation of Gel-Formation
Processes. K. Higaki1, Y. Sasakura1, I. Hachiya1, S. Ueno2, and K. Sato2, 1Meiji Seika Kaisha
Ltd., Japan; 2Faculty of Applied Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. III: Rheological Properties. K. Higaki1, T.
Koyano1, I. Hachiya1, and K. Sato2, 1Meiji Seika Kaisha Ltd., Japan; 2Faculty of Applied
Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. IV: Why and How? K. Sato1, K. Higaki2, T.
Koyano2, and I. Hachiya2, 1Faculty of Applied Biological Science, Hiroshima University, Japan;
2Meiji Seika Kaisha Ltd., Japan

Effect of Myofibrillar Proteins of Sea Salmon (Pseudopercis semifasciata)- Malondehyde Interaction of Microstructure and Functionality. V.A. Tironi, M.C. Tomas, M.C. Anon, 1CIDCA, Argentina

Nitrates and Nitrites in Food. A. Telniceanu, Institute of Hygiene and Public Health Bucharest, Romania

Microstructure, Stability and Viscoelastic Properties of Reduced Oil Content Emulsions Formulated with Polysaccharides and Salt. J.M. Quintana1, A.N. Califano1, N.E. Zaritsky1, P. Partal2. 1 CIDCA, CONICET, Universidad Nacional de La Plata Argentina; 2 Universidad de Huelva, Escuela Politécnica Superior, Spain

A comparative study of connective tissue components in bovine and fish muscles using histological techniques. G. Enersen, R. Ofstad, V. Høst, K.H. Eggen, Norwegian Food Research Institute, Norway.

Effects on Texture and Microstructure after Replacing Manual by Mechanical Processing in Serra da Estrela Cheesemaking. P.J.M. Reis, F.X. Malcata.
Escola Superior Biotecnologia - UCP, Portugal

Interfacial and Surface Behaviour of Protamine:BSA Mixed Systems. L.A. Glaser1, A.T. Paulson1, D. Rousseau2, 1Dalhousie University, Canada; 2Ryerson University, Canada

Soy protein isolate glycation and its effect on protein functionality. J. I. Boye1, P. Li2, J. Lenay3, V.A. Yaylayan2, F.K. Yeboah2, A. Achouri1; 1 Agriculture and Agri-Food Canada, Canada; 2 McGill University, Canada; 3University of Laval, Canada

The Role of Microstructure Changes in Microwave-Induced Toughness of Bread.
M. Uzzan, E. Kesselman, O. Ramon, S. Mizrahi, I.J. Kopelman. Technion, I.I. T, Israel.

*-------------------------------------------------------------------------------------------------------------------


Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca


From daemon Mon Feb 18 15:30:27 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 18 Feb 2002 13:26:47 -0800
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 06:23 AM 2/18/2002, you wrote:
} Gary Gaugler wrote:
}
} } Why can't the student respond? She already did.
}
} Sorry I missed that. However, my intension is not just
} to defend her.

Ah....then you have other intentions as well. OK.
No problem.


} } Q1 and Q2 are so basic that I can't see how one
} } would have to do much research to answer these
} } questions. Reviewing Goldstein, Newbury, Echlin,
} } et.al. should do the job.

The basic differentiating factor is the wavelength of
light compared to that of electrons. As I "see" it.
The shorter the wavelength used, the greater is the
resolving power. Resolving power (resolution) is
the ability to distinguish separation of two objects
which are separated by a small distance. For LM,
use RP=Lambda/2NA where Lambda is the wavelength
of light, NA is the numerical aperture of the objective.
For EM, use ThetaR=1.22Lambda/d. This angle,
ThetaR, is the minimum angular separation between
two objects based on wavelength and d, the diameter
of the converging lens.

Thus, to increase resolution, either increase the
diameter of the converging lens, use a shorter
wavelength, or both. For light, 5500A is a good
figure for its wavelength. What about the wavelength
of electrons? One can take the particle view or
the wave view. Since light is of the wave nature,
we can similarly treat electrons. Huygens originally
proposed the wave theory of light, in contrast to
the particle theory of E=hv. De Broglie postulated
that the wavelength of matter waves was the same
as for light. This is given as Lambda=h/p which
relates wavelength to momentum of the associated
photons. Thus the duality of waves (Lambda and v)
and that of particles (E and p). In some circumstances,
light and electrons (matter) behave like a particle
and in others, like a wave.

The wavelength of electrons can be computed
using either the de Broglie relationship or the
Bragg relationship. But if one uses the de Broglie
relationship, which is based on momentum,
then an interesting facet is exposed. That is, that
the wavelength of electrons or an electron beam
is dependent on its kinetic energy. Thus, the
higher the KV of the beam, the shorter the wavelength
and hence, better resolution. All other things being
equal, of course.

Another advantage of the SEM is that because of its
shorter wavelength of electrons, it supports a much
greater working distance than does LM. But that's
another story--if not a big difference between LM and SEM.

} Q3 might be a bit more involved.
}
} So you do think Q3 is not that simple.

Not for me. But here is some info:

http://www.nasatech.com/Briefs/May01/NPO21056.html

http://www.eun.org/kms/sites/eschola/view_leadingedge.cfm?oid=1714

http://www-celanphy.sci.kun.nl/Bruce%20web/scanning%20microscopy.htm



} } I think that at least 99 out of 100 experts would
} } answer Q1 and Q2 the same. At least, I would hope so.
}
} Let's assume the student receive two answers(since so many
} of us refuse to answer). One of them happen to be is coming from
} that 1 out 100. So to her, she will have two opposite answer
} without knowing another 98 expert's opinion. What could she do?

She might actually have to do some on-line research
to adjudicate the differences. Or, she may form her own
hypothesis. Or she could give up.


} } Perhaps Raleigh should be contacted?
}
} That is exactly what I am planning to do! - if some one care to
} post their answer. Will you? Please?

Sure. See above.

BTW, what did Raleigh have to say about this topic?

gary g.



From daemon Mon Feb 18 20:02:25 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 18 Feb 2002 19:53:42 -0600
Subject: repolishing EDS standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a SEM planchette containing 52 EDS standards that is in need
of repolishing. It was originally made by C.M. Taylor Corporation of
Stanford, CA but I am sure that other companies may be able to
repolish it. I would appreciate receiving any information on this
possibility.

Is it possible to do this ourselves? What would be involved?

Many thanks,

John B.


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Mon Feb 18 21:40:17 2002



From: xf200-at-attbi.com
Date: Mon, 18 Feb 2002 22:32:48 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gary,

Thank you so much for give the answer.
I presume it is for Q2. How about Q1 and Q3?

Again, imagine I am the student, this is the conclusion I can get
from your answer(after thinking, not just repeating you):

"If a light wave is about 500nm, I can expect the resolution about
250nm.
Correct? Or maybe close?

} From your instruction on electron wave, I can calculate for a 10kv
electron,
the wavelength is about 0.013nm. Do I expect the resolution is about
that?
Maybe a little worse, 0.02nm? Or much worse, 0.2nm? Even at this
resolution,
I would be able to see single atom, right?
I am very optimistic about this, even it is 100 times worse, I still
have 2nm resolution.
But there are much rooms to improve, if I wish to have 0.2nm resolution,
all I have
to do is to increase the energy by 100 times, so at 1000kv, I will have
0.2nm resolution
and capable of seeing atoms, right?"

Could you please post the answer for Q1 and Q3, please? If no one else
cares,
at least I wish to learn.

I am completely surprised about the website you suggested on Q3. I
though this
is a "Question on electron microscope", not light microscope(see the
subject title).
If it is not restricted in electron microcopy, why should it be
restricted in light
microscopy? I am sure the term "temporal resolution" is used in many
field.

Now can you see the point I am trying to make? These questions seems
simple,
but not all of us can answer it correctly, or at least without
misleading(myself
can be an example).

} } Gary Gaugler wrote:
}
} The basic differentiating factor is the wavelength of
} light compared to that of electrons. As I "see" it.
} The shorter the wavelength used, the greater is the
} resolving power. Resolving power (resolution) is
} the ability to distinguish separation of two objects
} which are separated by a small distance. For LM,
} use RP=Lambda/2NA where Lambda is the wavelength
} of light, NA is the numerical aperture of the objective.
} For EM, use ThetaR=1.22Lambda/d. This angle,
} ThetaR, is the minimum angular separation between
} two objects based on wavelength and d, the diameter
} of the converging lens.
}
} Thus, to increase resolution, either increase the
} diameter of the converging lens, use a shorter
} wavelength, or both. For light, 5500A is a good
} figure for its wavelength. What about the wavelength
} of electrons? One can take the particle view or
} the wave view. Since light is of the wave nature,
} we can similarly treat electrons. Huygens originally
} proposed the wave theory of light, in contrast to
} the particle theory of E=hv. De Broglie postulated
} that the wavelength of matter waves was the same
} as for light. This is given as Lambda=h/p which
} relates wavelength to momentum of the associated
} photons. Thus the duality of waves (Lambda and v)
} and that of particles (E and p). In some circumstances,
} light and electrons (matter) behave like a particle
} and in others, like a wave.
}
} The wavelength of electrons can be computed
} using either the de Broglie relationship or the
} Bragg relationship. But if one uses the de Broglie
} relationship, which is based on momentum,
} then an interesting facet is exposed. That is, that
} the wavelength of electrons or an electron beam
} is dependent on its kinetic energy. Thus, the
} higher the KV of the beam, the shorter the wavelength
} and hence, better resolution. All other things being
} equal, of course.
}
} Another advantage of the SEM is that because of its
} shorter wavelength of electrons, it supports a much
} greater working distance than does LM. But that's
} another story--if not a big difference between LM and SEM.
}
} } Q3 might be a bit more involved.
} }
} } So you do think Q3 is not that simple.
}
} Not for me. But here is some info:
}
} http://www.nasatech.com/Briefs/May01/NPO21056.html
}
} http://www.eun.org/kms/sites/eschola/view_leadingedge.cfm?oid=1714
}
} http://www-celanphy.sci.kun.nl/Bruce%20web/scanning%20microscopy.htm




From daemon Tue Feb 19 00:04:34 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 18 Feb 2002 21:58:33 -0800
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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At 07:32 PM 2/18/2002, you wrote:
} Dear Gary,
}
} Thank you so much for give the answer.
} I presume it is for Q2. How about Q1 and Q3?

As far as I know, I answered all questions....
1-3. At least, as best I could.

Were you able to contact Raleigh? That would
be quite helpful.

} Again, imagine I am the student, this is the conclusion I can get
} from your answer(after thinking, not just repeating you):
}
} "If a light wave is about 500nm, I can expect the resolution about
} 250nm.
} Correct? Or maybe close?

Not so, as I see it. 550nm (5500A) is the wavelength,
but the resolution is dependent on the optics. How do you
account for this? NA plays a major part of this facet. So
does the condenser.

} } From your instruction on electron wave, I can calculate for a 10kv
} electron,
} the wavelength is about 0.013nm. Do I expect the resolution is about
} that?

How do you calculate that? I do not deny that figure, I just
would like to know how you arrived at that figure. Let me
see the work.

} Maybe a little worse, 0.02nm? Or much worse, 0.2nm? Even at this
} resolution,
} I would be able to see single atom, right?
} I am very optimistic about this, even it is 100 times worse, I still
} have 2nm resolution.
} But there are much rooms to improve, if I wish to have 0.2nm resolution,
} all I have
} to do is to increase the energy by 100 times, so at 1000kv, I will have
} 0.2nm resolution
} and capable of seeing atoms, right?"

Theoretically. But practically, who makes a SEM with 1MEV
potential?? None that I know of. Even with TEM, 300KEV is
quite high. But the difference of looking through or at a
specimen is quite different...I think. It is morphology versus
structure. Different.

But there is a more fundamental issue here. That is
the distance of scan line dimensions. Say what??
Well, if the probe size is too large, it will overshadow
the specimen. If it is too small, it will miss features
of the specimen.

There are critical factors which affect the ultimate
resolution of an EM. These are spacial aberration,
chromatic aberration, and diffraction. But wait...there
is more....diffraction limitations!! Aperture size. This
affects intensity distribution and resolves on the
disc of least confusion. Ah...but this brings us back to
resolution. This is somewhat spot size (probe diameter)
limited. If the spot size is too large, the scan will miss
intermediate detail. If too small, it will also miss detail.
The issue I think is the relationship of free working
distance versus optical working distance. Both can be
changed. Free WD is from the pole piece to the
specimen while optical WD is the distance from the
final aperture to the specimen. This is reflected in
novel final lens designs.


} Could you please post the answer for Q1 and Q3, please? If no one else
} cares, at least I wish to learn.

I believe that I did answer all three questions....in my own way.


} I am completely surprised about the website you suggested on Q3. I
} though this
} is a "Question on electron microscope", not light microscope(see the
} subject title).
} If it is not restricted in electron microcopy, why should it be
} restricted in light
} microscopy? I am sure the term "temporal resolution" is used in many
} field.

Ah...therein lies the subtleties of the major issue. Unfortunately,
it is not constrained to one venue. Were it not so, that would be
terrific. Read and absorb the basic issue about temporal
resolution. It seems to me that it is rather basic and
intuitively obvious, once examined. Why would it make any
difference between LM and EM, other than the inherent limitations
therein?


} Now can you see the point I am trying to make? These questions seems
} simple, but not all of us can answer it correctly, or at least without
} misleading(myself can be an example).

Simple questions, per se, do not engender simple answers
at all times. Such is the stuff of quantum mechanics and
wave theory. But this is particularly different from Shrodinger,
de Broglie and Bragg. Too many "negative waves," as was
said in a notable video (Sutherland).

Gary Gaugler, Ph.D.



From daemon Tue Feb 19 02:31:48 2002



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Tue, 19 Feb 2002 00:20:58 -0800 (PST)
Subject: RE: Questions on the Electron Microscope

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On Sat, 16 Feb 2002 xf200-at-attbi.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} It is not fair that the student does not have any chance to respond.
}
} If I were that student, here is my respond:
}
} You may think that giving me straight answer seems too easy on me. But
} on my side, it is not that simple to use your answer. If I ask my
} professor directly to get an answer, as long as I can repeat his/her
} words like 3 years old, I can get a A, even if he is WRONG.
}
} But asking you is completely different. You could be WRONG too! If I
} took your wrong answer straight to my professor, I will fail. No
} matter how I explain this is from some "expert", it is not going to
} help.
}
} Oh, how could YOU be wrong? Isn't that very insulting since you are
} the expert? If you felt that way, I am sorry. I did not mean to insult
} any one. However, how about post your answer to these "elementary"
} questions on this list server? Do you think everyone on this list will
} agree with you?
}
} Here are the re-post of Jenny Wang's original question:
} 1. What are the advantages and disadvantages in using
} electrons for microscopy rather than light?
} 2. Does the wavelength of the electrons have anything
} to do with the spatial resolution that the microscope
} produces in the final picture?
} 3. What is temporal resolution and how is it produced
} in the electron microscope?
}
} Thank you very much in deed.
}
To coincide with Gary's comments I put forth my own:

Resolution in the transmission electron microscope (TEM) and the optical
light microscope (OLM) are generally governed by the same laws of optical
physics. Namely, the shorter the wavelength, the better the resolution.
Thus, the advantage to the TEM would in fact be the ability to resolution
smaller structures than the OLM.

The first issue is to define resolution. The second step is to define
wavelength utilitzed, and the three step is to define numerical aperture.
Once you have defined and understand these terms, only then will you
realize how varied are your choices in drawing a final conclusion about
your three questions.

An analogy would be to ask you, "Which is better, an airplane or a car",
as it relates to transportation? Each have advantages and disadvantages.

I have written about wavelength and numerical aperture as they relate to
resolution. However, you must keep in mind, as with transportation, there
are a number of ways of getting around, so are there many caveats to
adhering solely to these two perimeters.

Let's take for example Abbe's formula for OLM resolution. He states
resolution is basically half the wavelength utilized. Consequently, if we
use 500nm light, half would be 250nm resolution. The formula would be:
resolution (d) = wavelength over 2 x numerical aperture (the n.a. of the
condenser lens and the objective). If you take the numerical aperture of
the condenser lens as 1.4 and the objective lens as 1.4 = 1.96 divided by
the wavelength utilized (500nm) the resolution would be 255nm, or pretty
close to Abbe's generalization of resolution equals half the wavelength
utilitzed.

Now let's consider a 40x objective lens (n.a. 0.70) and the condenser lens
(1.40). The resolution would be 510nm. Take a slide mounted with
diatomaceous earth or perhaps a scrapping from the inside of your mouth
and, using brightfield, tell me if you can resolve structures at 510nm?
The issue is contrast! You cannot resolve the structures because these
specimens are phase objects and not amplitude producing objects. The only
way you may be able to spatially resolve any structure is to reduce the
condenser aperture. You must reduce it to the point where in affect you
reduce the annular aperture and thus, destroy the numerical aperture, one
of the key components to Abbe's formula for resolution!

How do we increase the amplitude or contrast so as not to affect the n.a.?
Well, darkfield illumination, phase contrast illumination, and
differential interference contrast (DIC) illumination might be a start.
The other method would be to stain the material, which would be difficult
with diatomaceous earth!

These techniques are the hallmark for OLM, as they are methods of changing
the amplitude through color as in staining the material, or refractive
index as in darkfield, and phase/refractive indices as in phase contrast
or DIC.

However, what happens when we want to observe structures smaller than
250nm? Yes, we can use fluorescence and gain slightly better resolution.
However, we must resort to electrons...

The issue with the TEM and electron is not dissimilar to our issue with
photons in the OLM. Spatial resolution is governed by sample thickness
and accelerating voltage. If one uses an accelerating voltage of 100kV at
a wavelight of 0.037, the resolving power is about 0.17nm.

However, let's decrease the kV to 50kV or place a thicker sample into
electron beam. The consequence is an increase in scattered electrons,
which equals less signal above background. Signal is good, background
noise is bad. If we decrease our kV we get more contrast but less
resolution, i.e., more amplitude through reduced resolution.

Spatial and temporal resolution are clearly more difficult to discuss in
this space or my time. Again, sample thickness, accelerating voltage, and
condenser/objective apertures (and even degree of vacuum) will play
differing roles in the outcome of resolution. Then the question arises as
to how you are going to document this resolution. It is visual,
photographic, or digital/video?

Based on this information, do you have questions? Perhaps this
information and that provided by others will help with the anwsers.
However, who gets the grade for effort. Tell us (the members of the
listserver) what you believe to be the correct answer(s). It is far
easier to help through thoughtful questions as opposed to spewing out
everything one knows on a given subject.

Ken
______________
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203







From daemon Tue Feb 19 05:48:17 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 19 Feb 2002 06:37:40 -0500
Subject: C. M. Taylor Standards

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

John Bozolla wrote:
==============================================================
We have a SEM planchette containing 52 EDS standards that is in need of
repolishing. It was originally made by C.M. Taylor Corporation of Stanford,
CA but I am sure that other companies may be able to repolish it. I would
appreciate receiving any information on this possibility.

Is it possible to do this ourselves? What would be involved?
==============================================================
SPI Supplies has been offering a standards "refurbishing" service for those
with standards made by the C. M. Taylor Company (which by the way, does not
exist any more). The prices are only slightly higher than those listed for
the refurbishing of the SPI Standards, see URL
http://www.2spi.com/catalog/standards/refurb.html

We have also acquired a large library of the original standards that were
originally characterized by Dr. Taylor himself, and thererfore, if one or
more of the individual standard items has fallen out, or comes out during
the refurbishing, it can be replaced with essentially the identical standard

From daemon Tue Feb 19 06:58:16 2002



From: r.cross-at-ru.ac.za
Date: Tue, 19 Feb 2002 14:52:26 +0200
Subject: ICEM news update

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ICEM-15 NEWS UPDATE

It is just over six months before ICEM-15 opens in Durban.
Delegates are now registering and submitting abstracts,
symposia chairs from throughout the world are busy organizing
their symposia and awaiting the abstracts to see what has been
submitted for their symposia, the invited speakers have been
selected and are preparing their presentations, and in Durban,
and elsewhere, the various members of the Organizing
Committee and their helpers are doing whatever is necessary to
make sure that their contribution towards this project is done
properly and on time. Everything is in place, therefore, to make
sure that delegates enjoy a most memorable and successful
congress.

The Scientific Programme Committee, co-chaired by Mike
Witcomb and Trevor Sewell, assisted by several key members of
MSSA, the International Scientific Advisory Committee and the
IFSEM executive, have put together what will be a wide-ranging
and very interesting collection of topics into over 50 scientific
symposia, four plenary sessions, the Ernst Abbe Lecture and the
IFSEM Symposium. There will be at least 160 invited lectures by
experts on these subjects, and because these speakers come
from throughout the world, this will be a truly international
meeting. The deadline for receipt of abstracts for the scientific
symposia is 25 February.

The Technical Forum will provide an excellent opportunity for
presentation and discussion of matters of a technical,
commercial, managerial and administrative nature, in a pleasantly
relaxed atmosphere that is conducive to fruitful discussion. This
is something new for ICEM and it is hoped that it will be
popular with many delegates especially managers, technicians,
students and representatives of the trade. Requirements for
abstracts of the Technical Forum are much less stringent than
for the scientific symposia, and the deadline for receipt of
these in Durban is 1 April, and thus allows delegates with
“breaking news” quickly to present their results and get their
abstracts in print!

The Trade Exhibition at ICEM-15 promises to provide an
exciting array of instruments, accessories and consumables. At
least 35 companies have reserved stands on the exhibition and a
look at the exhibition plan on the website reveals that at this
stage there are only 5 regular stands and a few display stands
still available. The co-operation of the trade in making their
arrangements so early is much appreciated.

The Local Organizing Committee and the Event Manager,
Turners Conferences, are continuing with their impressive
programme that is designed to ensure that all delegates,
representatives, families and friends from wherever they may
be leave Durban after ICEM-15 having happy memories of a most
rewarding and enjoyable congress. Anyone doubting that Durban,
or South Africa, has the ability to cater for events such as
ICEM-15 can take heart from the success of two recent major
international conferences that took place at ICC Durban, namely
the world conferences on racism and HIV/AIDS. These both
had registrations of 10 000 to 20 000 delegates and very few
difficulties were experienced by delegates or the organizers.
Further confidence has been expressed in South Africa’s record
in handling international conferences through the award of the
World Conference on Sustainable Development (Earth Summit
2002) that takes place in Johannesburg shortly before ICEM-
15. It has been reported that 194 heads of state will attend this
meeting along with over 50 000 other delegates!

A special bonus for delegates is that due to recent movements
in world currency markets the South African Rand has devalued
significantly against most world currencies. This means that your
dollars, euros, pesos, krones, etc, will all buy much more in the
way of goods and services. Accommodation, even the very best,
will appear to be ridiculously cheap by international standards,
and not to be missed are the shopping opportunities, gourmet
meals, wild life safaris, etc, that will represent amazing value for
money before, during and after ICEM-15. BMW reported
yesterday that its products are 25% cheaper in South Africa
than anywhere else in the world so if you have room then now is
the time to buy your new BMW! Accommodation is available to
suit all tastes and budgets but prospective delegates are warned
that the more popular hotels are filling up. Special rates have
been negotiated with the official congress hotels, and these
rates can be seen on the Turner’s web site
(www.turners.co.za/icem15) and were included in the Second
Circular and Call for Abstracts. Remember that today $US1.00
buys 11.50 SA Rands, 1 Euro buys 10 SA Rands and GBP1.00 buys
16.5 SA Rands.

South Africans are well known for their hospitality.
Consequently the programme for social events has been well
thought out and will provide something exciting, different and
enjoyable for all ICEM-15 delegates, families and friends.
Because of features for which South Africa is famous such as
its cultural diversity, natural resources and great weather the
social programme that goes with ICEM-15 will be an experience
not to be missed. Details of all these activities are available on
the web sites:

www.icem15.com
www.turners.co.za/icem15

Getting to Durban, and around South Africa, is uncomplicated
and surprisingly inexpensive when compared to Europe, North
America and the East, especially if arrangements are made well
in advance. As the “Earth Summit” is being held in Johannesburg
shortly before ICEM-15 it is all the more important for ICEM-
15 delegates to make their travel arrangements well in advance.
There are many flights daily by most of the world’s major
airlines from Europe, North and South America, the East and
Australasia to Durban, either directly or via Johannesburg or
Cape Town. Travel networks within South Africa, whether air,
road or rail, are well developed and by air most major
destinations can be reached from Durban within two hours,
making use of up-to-date fleets of aircraft used by South
African Airways and other domestic airlines. Airport formalities
and transfers are straightforward, and to give delegates an
early welcome (and be there for anyone requiring advice or
assistance) the ICEM Organizing Committee will have welcome
desks in the arrivals halls of Johannesburg, Cape Town and
Durban International Airports.

The Event Managers for ICEM-15, Turners Conferences and
Conventions, will be pleased to answer any enquiries about
flights, tours, accommodation, car hire, etc, and have negotiated
the most favourable rates for ICEM-15 delegates. Delegates
who have already made their bookings through Turners are very
pleased with the competitive fares that they have been offered.
For more information contact Mr Dudley Randall (Turners
Conferences) by email at turner17-at-galileosa.co.za

Look also the following web sites for more relevant
information:
http://www.icem15.com (official ICEM-15 web site)
http://www.turners.co.za/icem15 (event management web site)
http://www.uct.ac.za/depts/emu/mssa (MSSA web site)
http://www.materials.ox.ac.uk/ifsem (IFSEM web site)
http://www.satour.com (SA Tourism web site)
http://southafrica.net (tourist information web site)
http://www.kwazulu-natal.co.za (Durban and surrounding area
tourist information)

The ICEM-15 Organizing Committee looks forward to welcoming
you to Durban in September 2002. It will be an experience not
to be missed.

***


From daemon Tue Feb 19 08:55:19 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 19 Feb 2002 09:42:36 -0500
Subject: Re: TEM of B-galactosidase

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Dorothy,
I have looked at beta-gal in the EM (Cohen-Gould & Mikawa,
Developmental Biol 177(1) 265-276, 1996).
the X-gal crystals are very easy to see in the EM. The main caveat,
is that the embedding resing doesn't penetrate the crystals, so that
your sections tend to tear under the beam. I used parlodion or
formvar coated grids to add support and that helped a lot.
Good luck,
Lee
--
Lee Cohen-Gould
Electron & Optical Microscopy Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207


From daemon Tue Feb 19 09:14:45 2002



From: CerTemGT-at-aol.com
Date: Tue, 19 Feb 2002 10:08:42 EST
Subject: EDS system for TEM

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We looking for an EDS system in good working condition with horizontal entry detector (Be window), electronics console, monitor, and printer. We prefer a system that can be directly mounted on a Philips TEM but other systems could work.


From daemon Tue Feb 19 10:18:46 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Tue, 19 Feb 2002 11:21:00 -0500
Subject: Re: repolishing EDS standards

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The large variation in hardness between the different standards is
probablythe biggest obstacle in attempting this yourself. If another
standards supplier will guarantee results you should strongly consider
that. Otherwise, if you have someone withexperience in critical
metallurgical preparation, who also has experiencewith nonmetallics such
as ceramics, you might enlist their help. It would not be wise to learn
on your standards.

The nature of the problem to be corrected will determine the steps to
take. You should use the minimum polish size possible in order to avoid
creating new problems. If your intention is merely to remove beam
contamination, 1 micron alumina should be more than adequate and you
will need to follow that with at least one step of finer polishing. If
the standards have been damaged by excessive beam currents so that the
existing surface needs to be removed, you will need to deal with the
problem of relief development among materials of differing hardness.

In general, to avoid this it is best to use an abrasive of high
efficiency on a napless cloth. 3 micron diamond on woven nylon would be
an example. The time and pressure should be kept to a minimum in order
to avoid rounding of edges and embedding of abrasive in softer
standards, respectively. You will have to follow this with exhaustive
cleaning followed by polishing steps. This regimen has worked well for
me with composite assemblies involving gold (very malleable), soft
solder (malleable but with some brittle intermetallic compounds),
plating layers (which must not be deformed), steel (prone to rapid
oxidation), and 96% alumina/ 4% silica ceramic (hardness nearly Mhos 9)
in the same sample.

The final issue is what polishing fluid to use. Years ago it was
alleged that some of the standards suppliers used olive oil, or a
mixture containing that, rather than something more "high tech". Olive
oil is mostly oleic acid, so one would assume that it has some detergent
properties as well as the capability to form fatty acid soaps with a
wide variety of metals, including most copper alloys. So while it may
offer some advantages, it is not inert, and should be used intelligently.

Removal of the last traces of very fine polish may be difficult from
some surfaces. In my experience a detergent that functions in a
non-aqueous medium is very helpful if used (briefly!) with ultrasonic
agitation. Potassium methyl cyclohexyl oleate (Vulpex) in naphtha is
compatible with most standards materials but you should expect to wash
repeatedly in clean solvent and dry under vacuum.

You should take special precautions with certain standards. For
example, some sulfides, arsenides and selenides are susceptible to polar
organic solvents that might come to mind as rinse agents.

I would be interested, as well, in hearing from anyone on the list who
has successfully reworked their standards or provides this service.

John Twilley
Conservation Scientist

John J. Bozzola wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We have a SEM planchette containing 52 EDS standards that is in need
} of repolishing. It was originally made by C.M. Taylor Corporation of
} Stanford, CA but I am sure that other companies may be able to
} repolish it. I would appreciate receiving any information on this
} possibility.
}
} Is it possible to do this ourselves? What would be involved?
}
} Many thanks,
}
} John B.
}
}



From daemon Tue Feb 19 11:00:55 2002



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Tue, 19 Feb 2002 09:47:06 -0700
Subject: Position

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Second Notice


Research Professional in Electron Microscopy
Center for High Resolution Electron Microscopy
Arizona State University

The Center for Solid State Science seeks applicants for the position of
Assistant/Associate/ Senior Research Professional. This appointment is a
state-funded Academic Professional position on a year-to-year basis.
Essential job functions of this position include: design and engineering of
electronic circuits for analog and digital functions; working closely with
faculty and students to design and manufacture items for research and
development; testing circuit functions to the component level using direct
and indirect trouble-shooting methods for failure diagnosis; and diagnosing
and repairing vacuum and mechanical systems

Required Qualifications: Master's degree in physical or engineering
sciences and five years of experience in electronic repair and maintenance
of analytical equipment; or Bachelor's degree in physical or engineering
sciences and 8 years of experience in electronic repair and maintenance of
analytical equipment. Associate and Full Research Professional ranks also
require a Doctorate degree in related area and/or additional extensive
experience appropriate to rank.

Desired Qualifications:
Š Previous experience with electron microscopes
Š Experience with low and high power distribution systems
Š Demonstrated working knowledge of electron microscopy maintenance and repair

Further information about the Center for High Resolution Electron
Microscopy can be found at www.asu.edu/clas/csss/chrem.

Applicants must submit a cover letter, resume/vitae with names, addresses,
phone numbers and email addresses for three professional references to:
CSSS Research Professional Search Committee, Center for Solid State
Science, PO Box 1704, Arizona State University, Tempe Arizona, 85287-1704.
Application deadline is January 28, 2002, or each Monday thereafter until
position is filled.


Arizona State University is an AA/EO employer




John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu



John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Tue Feb 19 11:01:17 2002



From: Vickie Frohlich :      frohlich-at-uthscsa.edu
Date: Tue, 19 Feb 2002 11:00:40 -0600
Subject: Optical Microscopy Course Announcement

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The University of Texas Health Science Center at San Antonio (UTHSCSA)

with Support from Hamamatsu Photonics KK

will offer a course on

"Optical Microscopy in the Biological Sciences"

June 5-12, 2002
Application Deadline -March 1, 2002


Tuition - $1,750 (includes room and board) $1300 (without room)
$$$$$ Limited number of complete scholarships are available $$$$$


Topics to be covered:
Microscope Optics: Phase Contrast, Dark-field, DIC, Polarization
Detectors * Digital Processing * Fluorescence Filters and Probes
Live Cell Imaging * FRET * FLIM * Green Fluorescent Proteins
Confocal * Multiphoton * Deconvolution * 3-D Reconstruction

Faculty:
Robert Blystone, Trinity University * Victoria Centonze Frohlich, UTHSCSA
* Robert Hard, SUNY-Buffalo
Brian Herman, UTHSCSA * Ernst Keller, Carl Zeiss * James Lechleiter, UTHSCSA
Kate Luby-Phelps, Medical College of Wisconsin * Masafumi Oshiro, Hamamatsu
Photonics KK
Peter So, MIT * Kenneth Spring, NIH * Simon Watkins, Univ. Pittsburgh

Participating Vendors:
Bio-Rad Inc., Carl Zeiss, Inc., Chroma Technology Corp., Coherent Laser
Group, Compix Inc., DVC Co. Inc., Hamamatsu Photonics Systems, Improvision
Inc., Intelligent Imaging Innovations, Leica Inc./Meyer Instruments, Inc.,
Media Cybernetics, Molecular Probes, Nikon Inc., Olympus America, Inc.,
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From daemon Tue Feb 19 12:42:09 2002



From: Xudong Fan :      fanx-at-msu.edu
Date: Tue, 19 Feb 2002 13:37:35 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

Sorry I must have missed something. If you are the student and give the
professor some website as an answer, I doubt you will pass it. I know I
won't..
So anybody else can post the answer on Q3 - in some simple sentence?

You did say some advantage of EM over LM, but I was expecting
"better resolution", "expensiveness" which student can directly
relate to. I don't think they know what "working distance" means
if they have not used SEM before.

To figure out wavelength is not that difficult, just use the formula you
gave me. lambda = h/p (h is plank constant) p is momentum, = mv
v is the speed, as in E=mv^2/2, E is energy, = eV, V is potential(voltage).
So knowing V, we can get lambda. Of course you have to include
relativistic effect in electron mass m = m0/sqrt(1-(v/c)^2).
(By the way, this is a "fundamental" question too, but I am very happy
to answer it, it at least helps me refreshing my memory)

I do not think we have much disagreement on the resolution of LM.
The textbook says the resolution is about 200nm. Sure you can get
worse, but that is still in the same order of the wavelength.

It is greatly different in EM. It is hundreds of times worse. That's fine
considering the optics. But at least it should be proportional to
wavelength,
i.e. "shorter wavelength, better resolution". right?
This is NOT the case in SEM. It is not impossible to build a 1MeV SEM.
No one did that. Not even 100kv. Why? Simply because it does not improve
resolution! To shorten the wavelength, the only way to do is to increase
energy
(see the relation above).Increase energy will definitely decrease the
practical resolution. It might improve the spherical abberation. The
overall effect is that : shortening wavelength does not necessarily
improve resolution in SEM. Theoratically.

I could not find any definition of "temporal resolution"
in electron microscope textbook. So I thought "temporal resolution"
might relates to "temporal coherency" which effects resolution.
We believe that we need a better temporal coherent source to produce
better resolution. e.g. use very narrow energy distribution(through a very
stable
high voltage). However Lord Raleigh says:
"Incoherent source produce BETTER resolution than coherent source".

I found discussing these fundamental questions fascinating.

Gary Gaugler wrote:

} At 07:32 PM 2/18/2002, you wrote:
} } Dear Gary,
} }
} } Thank you so much for give the answer.
} } I presume it is for Q2. How about Q1 and Q3?
}
} As far as I know, I answered all questions....
} 1-3. At least, as best I could.
}
} Were you able to contact Raleigh? That would
} be quite helpful.
}
} } Again, imagine I am the student, this is the conclusion I can get
} } from your answer(after thinking, not just repeating you):
} }
} } "If a light wave is about 500nm, I can expect the resolution about
} } 250nm.
} } Correct? Or maybe close?
}
} Not so, as I see it. 550nm (5500A) is the wavelength,
} but the resolution is dependent on the optics. How do you
} account for this? NA plays a major part of this facet. So
} does the condenser.
}
} } } From your instruction on electron wave, I can calculate for a 10kv
} } electron,
} } the wavelength is about 0.013nm. Do I expect the resolution is about
} } that?
}
} How do you calculate that? I do not deny that figure, I just
} would like to know how you arrived at that figure. Let me
} see the work.
}
} } Maybe a little worse, 0.02nm? Or much worse, 0.2nm? Even at this
} } resolution,
} } I would be able to see single atom, right?
} } I am very optimistic about this, even it is 100 times worse, I still
} } have 2nm resolution.
} } But there are much rooms to improve, if I wish to have 0.2nm resolution,
} } all I have
} } to do is to increase the energy by 100 times, so at 1000kv, I will have
} } 0.2nm resolution
} } and capable of seeing atoms, right?"
}
} Theoretically. But practically, who makes a SEM with 1MEV
} potential?? None that I know of. Even with TEM, 300KEV is
} quite high. But the difference of looking through or at a
} specimen is quite different...I think. It is morphology versus
} structure. Different.
}
} But there is a more fundamental issue here. That is
} the distance of scan line dimensions. Say what??
} Well, if the probe size is too large, it will overshadow
} the specimen. If it is too small, it will miss features
} of the specimen.
}
} There are critical factors which affect the ultimate
} resolution of an EM. These are spacial aberration,
} chromatic aberration, and diffraction. But wait...there
} is more....diffraction limitations!! Aperture size. This
} affects intensity distribution and resolves on the
} disc of least confusion. Ah...but this brings us back to
} resolution. This is somewhat spot size (probe diameter)
} limited. If the spot size is too large, the scan will miss
} intermediate detail. If too small, it will also miss detail.
} The issue I think is the relationship of free working
} distance versus optical working distance. Both can be
} changed. Free WD is from the pole piece to the
} specimen while optical WD is the distance from the
} final aperture to the specimen. This is reflected in
} novel final lens designs.
}
} } Could you please post the answer for Q1 and Q3, please? If no one else
} } cares, at least I wish to learn.
}
} I believe that I did answer all three questions....in my own way.
}
} } I am completely surprised about the website you suggested on Q3. I
} } though this
} } is a "Question on electron microscope", not light microscope(see the
} } subject title).
} } If it is not restricted in electron microcopy, why should it be
} } restricted in light
} } microscopy? I am sure the term "temporal resolution" is used in many
} } field.
}
} Ah...therein lies the subtleties of the major issue. Unfortunately,
} it is not constrained to one venue. Were it not so, that would be
} terrific. Read and absorb the basic issue about temporal
} resolution. It seems to me that it is rather basic and
} intuitively obvious, once examined. Why would it make any
} difference between LM and EM, other than the inherent limitations
} therein?
}
} } Now can you see the point I am trying to make? These questions seems
} } simple, but not all of us can answer it correctly, or at least without
} } misleading(myself can be an example).
}
} Simple questions, per se, do not engender simple answers
} at all times. Such is the stuff of quantum mechanics and
} wave theory. But this is particularly different from Shrodinger,
} de Broglie and Bragg. Too many "negative waves," as was
} said in a notable video (Sutherland).
}
} Gary Gaugler, Ph.D.


From daemon Tue Feb 19 14:15:37 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 19 Feb 2002 14:01:45 -0600
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suppose it is good to go back and rehash some of things on a more
fundamental level. Even those of us who have been doing this for many years
get rusty and can use a refresher course.

It sounds like you are touching on another factor that practically
determines resolution, and that is the interaction volume - the scattering
of the beam once it encounters the specimen. I often tell students who ask
me about the probe diameter of the SEM that they are probably asking the
wrong question, especially when dealing with x-ray analyses. At probe
currents of less than 1 nA, the diameter of the probe is quite a bit
smaller than the x-ray interaction volume. Under those conditions, the
accelerating voltage is more critical in determining the chemical spatial
resolution than is the beam diameter. The student will need to be aware of
these other factors in addition to the wavelength limit of the resolution.

"Temporal resolution" is an ambiguous term to me, too. I replied to the
student asking if she was referring to the speed with which images could be
collected in order to observe changes in the sample. (Someone was
definitely asking about observing processes in cells. It may not have been
her.) I did not get a reply, but if my guess is correct, we then need to be
talking about signal-to-noise ratios. I can take high-resolution SEM
images, but it normally requires sampling over a fairly slow scan. TV rate
images can be obtained, but the noise becomes a very significant factor. It
seems to me that most (not all) LM images have much better S/N ratios.

The issue of documenting biological processes raised a whole other issue.
Most biological processes stop long before the sample is introduced into
the EM, except maybe for some special cases in an E-SEM. Certainly, the
intra-cellular processes are not visible in the EM.

I can see some worth to this whole exercise if the instructor is trying to
get the students to think through the strengths and weaknesses of EM vs. LM
and also SEM vs. TEM. However, it is always "interesting" to see these
questions come across the list. The intent of the exercise is not always
clear, and the questions are not always formulated well. But I guess it
provides some relief from the routine.

Warren

At 01:37 PM 2/19/02 -0500, you wrote:
} Gary,
}
} Sorry I must have missed something. If you are the student and give the
} professor some website as an answer, I doubt you will pass it. I know I
} won't..
} So anybody else can post the answer on Q3 - in some simple sentence?
}
} You did say some advantage of EM over LM, but I was expecting
} "better resolution", "expensiveness" which student can directly
} relate to. I don't think they know what "working distance" means
} if they have not used SEM before.
}
} To figure out wavelength is not that difficult, just use the formula you
} gave me. lambda = h/p (h is plank constant) p is momentum, = mv
} v is the speed, as in E=mv^2/2, E is energy, = eV, V is potential(voltage).
} So knowing V, we can get lambda. Of course you have to include
} relativistic effect in electron mass m = m0/sqrt(1-(v/c)^2).
} (By the way, this is a "fundamental" question too, but I am very happy
} to answer it, it at least helps me refreshing my memory)
}
} I do not think we have much disagreement on the resolution of LM.
} The textbook says the resolution is about 200nm. Sure you can get
} worse, but that is still in the same order of the wavelength.
}
} It is greatly different in EM. It is hundreds of times worse. That's fine
} considering the optics. But at least it should be proportional to
} wavelength,
} i.e. "shorter wavelength, better resolution". right?
} This is NOT the case in SEM. It is not impossible to build a 1MeV SEM.
} No one did that. Not even 100kv. Why? Simply because it does not improve
} resolution! To shorten the wavelength, the only way to do is to increase
} energy
} (see the relation above).Increase energy will definitely decrease the
} practical resolution. It might improve the spherical abberation. The
} overall effect is that : shortening wavelength does not necessarily
} improve resolution in SEM. Theoratically.
}
} I could not find any definition of "temporal resolution"
} in electron microscope textbook. So I thought "temporal resolution"
} might relates to "temporal coherency" which effects resolution.
} We believe that we need a better temporal coherent source to produce
} better resolution. e.g. use very narrow energy distribution(through a very
} stable
} high voltage). However Lord Raleigh says:
} "Incoherent source produce BETTER resolution than coherent source".
}
} I found discussing these fundamental questions fascinating.



From daemon Tue Feb 19 16:10:13 2002



From: Xudong Fan :      fanx-at-msu.edu
Date: Tue, 19 Feb 2002 17:05:44 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ken,

Thank you so much for your answer. I learned a lot.
My intention is not to show that I have better answer than other people.
In fact, it is quite the opposite. The intention is mostly it is
selfish - I wish to learn myself.

Anyway, if you wish to know my answer, here it is:
1. What are the advantages and disadvantages in using
electrons for microscopy rather than light?
Answer: Advantage:
Better resolution, Better depth of field/focus, etc
Disadvatage:
Expansive, more complicated sample preparation, etc.
However, I think these answers are misleading. Someone might think if she/he
have enough money, great effort, she/he can accomplish the tasks with light
microscope with better resolution simple by switching to electron microscope.

If I ask all the light microscopist, how many of your tasks can be simply
replaced
with electron microscopy - presume you have enough funding and man power?

If the professor wish to get the answer above, it is better re-phrased as:
"a. List the advantages and disadvantages of electron microscopy.
b. List the advantages and disadvantages of light microscopy?"

Like the analogy you ask , "Which is better, an airplane or a car",
as it relates to transportation? Each have advantages and disadvantages.
This is a perfect general question. You can compare them.
However, if you ask "What is the advantage of an airplan rather than
car if I wish to travel from A to B?" You might say "airplan is faster".
Now if you are late to work in the morning, would airplan help you?

I might be fussy. But to avoid misleading, any effort is worthwhile.

2. Does the wavelength of the electrons have anything
to do with the spatial resolution that the microscope
produces in the final picture?
Answer: Generally, the shorter the wavelength, the better the resolution.
This is particularly true in light microscope. The resolving power is about
the same magnitude as wavelength, more or less.
However, in electron microscope, there is some limit. Even everything
else is unchanged, e.g. optics and sample. Shortening wavelength does not
garantee
increase resolution. For example, in SEM, shorter wavelength means
higher voltage(no other way), which means increasing interaction volumn,
therefore it is also a factor of reducing resolution. A balance can be made
at certain voltage, in another word, wavelength. So there is an optimal
wavelength to produce best resolution in SEM.

By the way, in your following comments, at 100kv, the wavelength is 0.0037nm
(or 0.037A), compare to 0.17nm resolution. There is a huge difference between

light and electron microscope resolution relative to their wavelength.

3. What is temporal resolution and how is it produced
in the electron microscope?

Honestly, I don't know. I know what "temporal resolution" of human eye is
which is the base for motion picture. There are definitions in light
microscopy.
But I could not find anything in simple EM textbook.
There is a website given by Gary at:
http://www.nasatech.com/Briefs/May01/NPO21056.html
which tells the "The principle of stroboscopy would be extended to scanning
electron microscopy" where SEM have adquate temporal resolution.

Yours sincerely,
Xudong Fan
fanx-at-msu.edu

Ken Tiekotter wrote:

} To coincide with Gary's comments I put forth my own:
}
} Resolution in the transmission electron microscope (TEM) and the optical
} light microscope (OLM) are generally governed by the same laws of optical
} physics. Namely, the shorter the wavelength, the better the resolution.
} Thus, the advantage to the TEM would in fact be the ability to resolution
} smaller structures than the OLM.
}
} The first issue is to define resolution. The second step is to define
} wavelength utilitzed, and the three step is to define numerical aperture.
} Once you have defined and understand these terms, only then will you
} realize how varied are your choices in drawing a final conclusion about
} your three questions.
}
} An analogy would be to ask you, "Which is better, an airplane or a car",
} as it relates to transportation? Each have advantages and disadvantages.
}
} I have written about wavelength and numerical aperture as they relate to
} resolution. However, you must keep in mind, as with transportation, there
} are a number of ways of getting around, so are there many caveats to
} adhering solely to these two perimeters.
}
} Let's take for example Abbe's formula for OLM resolution. He states
} resolution is basically half the wavelength utilized. Consequently, if we
} use 500nm light, half would be 250nm resolution. The formula would be:
} resolution (d) = wavelength over 2 x numerical aperture (the n.a. of the
} condenser lens and the objective). If you take the numerical aperture of
} the condenser lens as 1.4 and the objective lens as 1.4 = 1.96 divided by
} the wavelength utilized (500nm) the resolution would be 255nm, or pretty
} close to Abbe's generalization of resolution equals half the wavelength
} utilitzed.
}
} Now let's consider a 40x objective lens (n.a. 0.70) and the condenser lens
} (1.40). The resolution would be 510nm. Take a slide mounted with
} diatomaceous earth or perhaps a scrapping from the inside of your mouth
} and, using brightfield, tell me if you can resolve structures at 510nm?
} The issue is contrast! You cannot resolve the structures because these
} specimens are phase objects and not amplitude producing objects. The only
} way you may be able to spatially resolve any structure is to reduce the
} condenser aperture. You must reduce it to the point where in affect you
} reduce the annular aperture and thus, destroy the numerical aperture, one
} of the key components to Abbe's formula for resolution!
}
} How do we increase the amplitude or contrast so as not to affect the n.a.?
} Well, darkfield illumination, phase contrast illumination, and
} differential interference contrast (DIC) illumination might be a start.
} The other method would be to stain the material, which would be difficult
} with diatomaceous earth!
}
} These techniques are the hallmark for OLM, as they are methods of changing
} the amplitude through color as in staining the material, or refractive
} index as in darkfield, and phase/refractive indices as in phase contrast
} or DIC.
}
} However, what happens when we want to observe structures smaller than
} 250nm? Yes, we can use fluorescence and gain slightly better resolution.
} However, we must resort to electrons...
}
} The issue with the TEM and electron is not dissimilar to our issue with
} photons in the OLM. Spatial resolution is governed by sample thickness
} and accelerating voltage. If one uses an accelerating voltage of 100kV at
} a wavelight of 0.037, the resolving power is about 0.17nm.
}
} However, let's decrease the kV to 50kV or place a thicker sample into
} electron beam. The consequence is an increase in scattered electrons,
} which equals less signal above background. Signal is good, background
} noise is bad. If we decrease our kV we get more contrast but less
} resolution, i.e., more amplitude through reduced resolution.
}
} Spatial and temporal resolution are clearly more difficult to discuss in
} this space or my time. Again, sample thickness, accelerating voltage, and
} condenser/objective apertures (and even degree of vacuum) will play
} differing roles in the outcome of resolution. Then the question arises as
} to how you are going to document this resolution. It is visual,
} photographic, or digital/video?
}
} Based on this information, do you have questions? Perhaps this
} information and that provided by others will help with the anwsers.
} However, who gets the grade for effort. Tell us (the members of the
} listserver) what you believe to be the correct answer(s). It is far
} easier to help through thoughtful questions as opposed to spewing out
} everything one knows on a given subject.
}
} Ken
} ______________
} Ken Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N. Willamette Blvd.
} Portland, OR 97203



From daemon Tue Feb 19 17:58:23 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 19 Feb 2002 15:54:25 -0800
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:37 AM 2/19/2002, you wrote:
} Gary,
}
} Sorry I must have missed something. If you are the student and give the
} professor some website as an answer, I doubt you will pass it. I know I
} won't..
} So anybody else can post the answer on Q3 - in some simple sentence?

The URLs are not the answer. What is at the URLs is. You have
to read the material at the URLs to compose an answer for yourself.
Better still, look up the dictionary definition of "temporal" and
then read the material at the URLs. If you still don't get it,
let me know.


} You did say some advantage of EM over LM, but I was expecting
} "better resolution", "expensiveness" which student can directly
} relate to. I don't think they know what "working distance" means
} if they have not used SEM before.

I thought I did say better resolution and also working distance for SEM.
Working distance applies to LM and EM. At upper magnifications,
the LM user is well aware of this factor. I am primarily talking
about SEM specifically rather than TEM when discussing EM.
I'm not a TEM person. I do SEM and LM.


} To figure out wavelength is not that difficult, just use the formula you
} gave me. lambda = h/p (h is plank constant) p is momentum, = mv
} v is the speed, as in E=mv^2/2, E is energy, = eV, V is potential(voltage).
} So knowing V, we can get lambda. Of course you have to include
} relativistic effect in electron mass m = m0/sqrt(1-(v/c)^2).
} (By the way, this is a "fundamental" question too, but I am very happy
} to answer it, it at least helps me refreshing my memory)
}
} I do not think we have much disagreement on the resolution of LM.
} The textbook says the resolution is about 200nm. Sure you can get
} worse, but that is still in the same order of the wavelength.

There are new LM instruments out there, like confocal and scanning
confocal, which have excellent resolution for LM. But SEM still
beats them.


} It is greatly different in EM. It is hundreds of times worse. That's fine
} considering the optics. But at least it should be proportional to
} wavelength,
} i.e. "shorter wavelength, better resolution". right?
} This is NOT the case in SEM. It is not impossible to build a 1MeV SEM.
} No one did that. Not even 100kv. Why? Simply because it does not improve
} resolution! To shorten the wavelength, the only way to do is to increase
} energy
} (see the relation above).Increase energy will definitely decrease the
} practical resolution. It might improve the spherical abberation. The
} overall effect is that : shortening wavelength does not necessarily
} improve resolution in SEM. Theoratically.

Let's see. In SEM, resolution is inversely proportional to wavelength
of the electrons. Shorter wavelength, greater resolution, in general.
Wavelength of electrons is inversely proportional to energy. Higher
energy, shorter wavelength. So, higher voltage/energy, higher
resolution (more resolving power). My old SX-40 brochure says
that it had a resolution of 60A. Newer SEMs are better than that.
My SEM is newer than the SX-40 and is supposed to have a
resolution of about 40A. I guess so, but I can't measure it.
I tried but failed. I get about 120-180A at 15KV. And that's
an eyeball guess. I cannot find a perfectly sharp edge!

BTW, I've been told that 1MV TEMs do exist. Most SEMs
are up to 30KV.


} I could not find any definition of "temporal resolution"
} in electron microscope textbook. So I thought "temporal resolution"
} might relates to "temporal coherency" which effects resolution.
} We believe that we need a better temporal coherent source to produce
} better resolution. e.g. use very narrow energy distribution(through a very
} stable
} high voltage). However Lord Raleigh says:
} "Incoherent source produce BETTER resolution than coherent source".

That is a coherent statement but wrong temporal at this time.


} I found discussing these fundamental questions fascinating.

Cool.

gary g.



From daemon Tue Feb 19 18:19:57 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 19 Feb 2002 16:17:56 -0800
Subject: Re: ISI SX-40 Help w/ type of video signal?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've been thinking about this problem and have a
theoretical solution based on unknown hardware.

If the CCTV of the SX-40 is indeed RS-170, then
there is hope. The key is, it seems to me, is to
perform Kalman filter operations on each field and
frame until the completed image is good enough
to grab.

To do this would require a frame grabber board with
a controllable buffer or a software product that
would malloc() memory in the PC for one or
more buffers. Thus, the issue whether the final image
is grabbed from the frame grabber board or from
PC memory via the interface/control software.
But either way, it would yield a high quality
640x480 pixel image. This is a perfectly acceptable
size for most work. If higher rez is needed, then
the expensive stuff comes into play.

gary g.


At 10:27 AM 2/6/2002, you wrote:

} Dear Lister
}
} I would like to thank those who gave me some input to my first
} delimma.
}
} I found that there are a ton of different frame grabbers out
} there. What I dont know is what kind of output signal the scope produces, as
} far as my understanding of the ISI SX-40 SEM it puts a signal out to the
} CRT. Some of the venders' questions have been if it is an NTSC, PAL, or
} RS170 type signal.I am slightly familiar with the first two, but the latter I
} have no idea. I do know that there are a couple of "off the shelf" products
} from GW Electronics and Image Slave, but these setups are priced at
} $4000+. So what I am looking for is if someone
} can point me in the direction of where I can reseach this information.



From daemon Tue Feb 19 18:24:18 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 19 Feb 2002 16:22:10 -0800
Subject: Death by poisoning - curari@asu.edu

Contents Retrieved from Microscopy Listserver Archives
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Looks like this person died.

gary g.


} Your message cannot be delivered to the following recipients:
}
} Recipient address: wil1323-at-IMAP2.ASU.EDU
} Original address: curari-at-asu.edu
} Reason: Remote SMTP server has rejected address
} Diagnostic code: smtp;550 5.1.1 {wil1323-at-IMAP2.ASU.EDU} ... User unknown
} Remote system: dns;imap2.asu.edu
} (TCP|129.219.110.72|44165|129.219.110.75|7025)



From daemon Wed Feb 20 02:40:48 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Wed, 20 Feb 2002 08:33:21 -0000
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shorter wavelength=greater resolution may be the general rule for all
imaging systems using waves, but
in SEMs that is far from the whole story, and no commercial TEM comes
anywhere close to the theoretical resolution limit. TEM resolving
power is spoiled by lens aberrations. I remember a recent paper which
described a way of neutralising spherical aberrations in a TEM using
an electron mirror.

Early SEM guns and optics were designed for 30 kV, and were virtually
unusable at 1kV. SEM development since the 60s has been about
emitters, guns and optics. Now, with FEGSEMs we can see that although
the fundamental rules still apply, major improvements in overall
performance at low kV have been possible by using near-monochromatic
emitters. Resolution in uncoated light-element specimens is now seen
to be a trade-off between kV/wavelength and beam interaction volume.
In the surface of a potato starch grain, starch crystal edges can be
seen at 1 or 2kV, but these are obliterated at 5 or 10 kV as the beam
interaction volume grows. Resolution in TEM is also a function of
contrast, which is poorer at high kV, which is why some biological
TEMs are designed with long working distance optics, trading some
resolving power to buy higher contrast. EM instruments have greater
theoretical RESOLVING POWER at higher kV, but in practice the
RESOLUTION can be poorer. Resolving power is an instrument property.
Resolution can be a specimen or image property. The two do not always
coincide.

Chris

} Let's see. In SEM, resolution is inversely proportional to
wavelength
} of the electrons. Shorter wavelength, greater resolution, in
general.
} Wavelength of electrons is inversely proportional to energy. Higher
} energy, shorter wavelength. So, higher voltage/energy, higher
} resolution (more resolving power). My old SX-40 brochure says
} that it had a resolution of 60A. Newer SEMs are better than that.
} My SEM is newer than the SX-40 and is supposed to have a
} resolution of about 40A. I guess so, but I can't measure it.
} I tried but failed. I get about 120-180A at 15KV. And that's
} an eyeball guess. I cannot find a perfectly sharp edge!




From daemon Wed Feb 20 03:56:12 2002



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Wed, 20 Feb 2002 01:46:01 -0800 (PST)
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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On Tue, 19 Feb 2002, Xudong Fan wrote:

} Dear Ken,
}
} Thank you so much for your answer. I learned a lot.
} My intention is not to show that I have better answer than other people.
} In fact, it is quite the opposite. The intention is mostly it is
} selfish - I wish to learn myself.
}
} Anyway, if you wish to know my answer, here it is:
} 1. What are the advantages and disadvantages in using
} electrons for microscopy rather than light?
} Answer: Advantage:
} Better resolution, Better depth of field/focus, etc
} Disadvatage:
} Expansive, more complicated sample preparation, etc.
} However, I think these answers are misleading. Someone might think if she/he
} have enough money, great effort, she/he can accomplish the tasks with light
} microscope with better resolution simple by switching to electron microscope.

*You must specify TEM or SEM or STEM or ESEM. You cannot ask these
questions as to how they relate solely to "EM", as the answers relating to
physical design and the physics associated with their operation are unique
to each instrument.

You state "better depth of field/focus". Yes, in the SEM the scanning
probe instruments, depth of field/focus is important. However, depth of
field in a 90nm section for TEM is, as you can imagine, extremely small,
hence the advantage of accelerating voltage.

As for the disadvantages of EM as being labor intensive, have you any
knowledge of LM preparation or the difference between TEM and SEM
preparation? SEM preparation is a walk in the park compared to the rigors
necessary to preserve, examine, and document ultrastrutural resolution at
0.1nm.

As for throwing money at the issue, the issue is clear. Think of your
compliment of microscopes as a toolbox: each tool in the toolbox has a
specific function. Yes, you can drive a nail in the wall to hung a
picture with the handle of a screwdriver, however, the best tool would be
a hammer!

The light microscope does not have the resolution of a TEM or the depth of
focus of a SEM, however, can a SEM or TEM convert phase differences of
amplitude as the phase contrast OLM? Can the SEM or TEM differentiate
specific structures using histological stain producing color difference or
look at refractive index differences as in DIC: I think not.

However, the SEM can provide incredible information about surface
topography, provide elemental differention, and 500x the depth of focus of
an OLM. Can it image surface information: NO, and not a any kV or spot
size. And then there is the TEM, STEM, ESEM....

As for instrument expense, look into a multi-photon, scanning laser
confocal OLM microscope at $400k to $600K!

} If I ask all the light microscopist, how many of your tasks can be
} simply replaced with electron microscopy - presume you have enough
} funding and man power?

Toolbox...more expensive toys do not necessarily answer the simplest
questions

}
} If the professor wish to get the answer above, it is better re-phrased as:
} "a. List the advantages and disadvantages of electron microscopy.
} b. List the advantages and disadvantages of light microscopy?"
}
} Like the analogy you ask , "Which is better, an airplane or a car",
} as it relates to transportation? Each have advantages and disadvantages.
} This is a perfect general question. You can compare them.
} However, if you ask "What is the advantage of an airplan rather than
} car if I wish to travel from A to B?" You might say "airplan is faster".
} Now if you are late to work in the morning, would airplan help you?
}
} I might be fussy. But to avoid misleading, any effort is worthwhile.
}
} 2. Does the wavelength of the electrons have anything
} to do with the spatial resolution that the microscope
} produces in the final picture?
} Answer: Generally, the shorter the wavelength, the better the resolution.
} This is particularly true in light microscope. The resolving power is about
} the same magnitude as wavelength, more or less.

Do you not agree there is a limit to the resolution of the OLM regardless
of wavelength or with respect to wavelength? If the OLM resolution were
limitless with respect to wavelength, why bother with the TEM?


} However, in electron microscope, there is some limit. Even everything
} else is unchanged, e.g. optics and sample. Shortening wavelength does
} not garantee increase resolution. For example, in SEM, shorter
} wavelength means higher voltage(no other way), which means increasing
} interaction volumn, therefore it is also a factor of reducing
} resolution. A balance can be made at certain voltage, in another word,
} wavelength. So there is an optimal wavelength to produce best
} resolution in SEM.

In the TEM higher voltage IS resolution, or at least a key component.
}
} By the way, in your following comments, at 100kv, the wavelength is 0.0037nm
} (or 0.037A), compare to 0.17nm resolution. There is a huge difference between
} light and electron microscope resolution relative to their wavelength.
}
} 3. What is temporal resolution and how is it produced
} in the electron microscope?
}
} Honestly, I don't know. I know what "temporal resolution" of human eye
} is which is the base for motion picture. There are definitions in
} light microscopy. But I could not find anything in simple EM textbook.
} There is a website given by Gary at:
} http://www.nasatech.com/Briefs/May01/NPO21056.html which tells the
} "The principle of stroboscopy would be extended to scanning electron
} microscopy" where SEM have adquate temporal resolution.
}
} Yours sincerely,
} Xudong Fan
} fanx-at-msu.edu
}
Enough is enough...
kt
--
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203


} Ken Tiekotter wrote:
}
} } To coincide with Gary's comments I put forth my own:
} }
} } Resolution in the transmission electron microscope (TEM) and the optical
} } light microscope (OLM) are generally governed by the same laws of optical
} } physics. Namely, the shorter the wavelength, the better the resolution.
} } Thus, the advantage to the TEM would in fact be the ability to resolution
} } smaller structures than the OLM.
} }
} } The first issue is to define resolution. The second step is to define
} } wavelength utilitzed, and the three step is to define numerical aperture.
} } Once you have defined and understand these terms, only then will you
} } realize how varied are your choices in drawing a final conclusion about
} } your three questions.
} }
} } An analogy would be to ask you, "Which is better, an airplane or a car",
} } as it relates to transportation? Each have advantages and disadvantages.
} }
} } I have written about wavelength and numerical aperture as they relate to
} } resolution. However, you must keep in mind, as with transportation, there
} } are a number of ways of getting around, so are there many caveats to
} } adhering solely to these two perimeters.
} }
} } Let's take for example Abbe's formula for OLM resolution. He states
} } resolution is basically half the wavelength utilized. Consequently, if we
} } use 500nm light, half would be 250nm resolution. The formula would be:
} } resolution (d) = wavelength over 2 x numerical aperture (the n.a. of the
} } condenser lens and the objective). If you take the numerical aperture of
} } the condenser lens as 1.4 and the objective lens as 1.4 = 1.96 divided by
} } the wavelength utilized (500nm) the resolution would be 255nm, or pretty
} } close to Abbe's generalization of resolution equals half the wavelength
} } utilitzed.
} }
} } Now let's consider a 40x objective lens (n.a. 0.70) and the condenser lens
} } (1.40). The resolution would be 510nm. Take a slide mounted with
} } diatomaceous earth or perhaps a scrapping from the inside of your mouth
} } and, using brightfield, tell me if you can resolve structures at 510nm?
} } The issue is contrast! You cannot resolve the structures because these
} } specimens are phase objects and not amplitude producing objects. The only
} } way you may be able to spatially resolve any structure is to reduce the
} } condenser aperture. You must reduce it to the point where in affect you
} } reduce the annular aperture and thus, destroy the numerical aperture, one
} } of the key components to Abbe's formula for resolution!
} }
} } How do we increase the amplitude or contrast so as not to affect the n.a.?
} } Well, darkfield illumination, phase contrast illumination, and
} } differential interference contrast (DIC) illumination might be a start.
} } The other method would be to stain the material, which would be difficult
} } with diatomaceous earth!
} }
} } These techniques are the hallmark for OLM, as they are methods of changing
} } the amplitude through color as in staining the material, or refractive
} } index as in darkfield, and phase/refractive indices as in phase contrast
} } or DIC.
} }
} } However, what happens when we want to observe structures smaller than
} } 250nm? Yes, we can use fluorescence and gain slightly better resolution.
} } However, we must resort to electrons...
} }
} } The issue with the TEM and electron is not dissimilar to our issue with
} } photons in the OLM. Spatial resolution is governed by sample thickness
} } and accelerating voltage. If one uses an accelerating voltage of 100kV at
} } a wavelight of 0.037, the resolving power is about 0.17nm.
} }
} } However, let's decrease the kV to 50kV or place a thicker sample into
} } electron beam. The consequence is an increase in scattered electrons,
} } which equals less signal above background. Signal is good, background
} } noise is bad. If we decrease our kV we get more contrast but less
} } resolution, i.e., more amplitude through reduced resolution.
} }
} } Spatial and temporal resolution are clearly more difficult to discuss in
} } this space or my time. Again, sample thickness, accelerating voltage, and
} } condenser/objective apertures (and even degree of vacuum) will play
} } differing roles in the outcome of resolution. Then the question arises as
} } to how you are going to document this resolution. It is visual,
} } photographic, or digital/video?
} }
} } Based on this information, do you have questions? Perhaps this
} } information and that provided by others will help with the anwsers.
} } However, who gets the grade for effort. Tell us (the members of the
} } listserver) what you believe to be the correct answer(s). It is far
} } easier to help through thoughtful questions as opposed to spewing out
} } everything one knows on a given subject.
} }
} } Ken
} } ______________
} } Ken Tiekotter, Adjunct Professor
} } The University of Portland
} } Department of Biology
} } 5000 N. Willamette Blvd.
} } Portland, OR 97203
}





From daemon Wed Feb 20 07:49:21 2002



From: kathy lowe :      kjl226-at-vt.edu
Date: Wed, 20 Feb 2002 08:45:41 -0500
Subject: Zamboni's Solution

Contents Retrieved from Microscopy Listserver Archives
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I think I have it this time..
Could someone give me information on the fixative Zamboni's solution? I
need to know how it's made when and why it is used over another fixative.

Thank you.
Kathy


From daemon Wed Feb 20 07:55:00 2002



From: kathy lowe :      kjl226-at-vt.edu
Date: Wed, 20 Feb 2002 07:46:57 -0600
Subject: Zamboni's sloution Zamboni's sloution

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Hello,
I have a question about Zamboni's sloution. Can anyone tell me how to make
this fixative?
Kathy


From daemon Wed Feb 20 08:24:04 2002



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Wed, 20 Feb 2002 09:18:09 -0500 (EST)
Subject: Re: TEM of B-gal

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who responded with advice about TEM of B-galactosidase. I
was suprised and glad to find out that one can see B-gal reaction product
by TEM. Thanks for the references and helpful hints. I'll give it a try.

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu



From daemon Wed Feb 20 08:52:40 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 20 Feb 2002 11:34:44 -0400
Subject: Position

Contents Retrieved from Microscopy Listserver Archives
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Director: Research Projects (Director, Electron Microscope Facility)
Vacancy Number: 12595
Work Unit: Vice President for Research, Life Sciences Consortium
Location: University Park Campus
Grade: 24 Exempt

Responsible for enabling faculty to design and implement protocols
using transmission and scanning electron microscopes, as well as
image and analyze biological and nonbiological materials.
Responsible for the maintenance and operation of light microscopes,
digital camera interfaces for light and EM microscopy, computers and
sample preparation equipment. Train EM personnel and researchers in
sample preparation techniques and the efficient operation of
instrumentation; teach laboratory courses; develop fee structure; and
participate in the development of instrumentation grant proposals.
Requires a Master's degree (Ph.D. preferred) or equivalent in
Biology, Biochemistry, Molecular Biology or related field, plus two
years of previous experience in electron microscopy and two years of
a research program. Computer and interpersonal skills required.

Pennsylvania State University
Penn State is committed to affirmative action, equal opportunity and
the diversity of the workforce.
Application deadline is March 11, 2002.

Please email or FAX resume and cover letter to:

Judith Burns, Mgr. STF SVCS, jeb2-at-psu.edu
The Life Sciences Consortium
519 Wartik Lab
University Park, Pa 16802
FAX: (814) 863-1357
--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Wed Feb 20 09:00:21 2002



From: akc-at-umich.edu
Date: Wed, 20 Feb 2002 09:54:47 -0500
Subject: Re: Zamboni's Solution

Contents Retrieved from Microscopy Listserver Archives
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Zamboni fixative was initially described in an abstract (Zamboni L,
DeMartino, 1967, Buffered picric acid-formaldehyde: a new, rapid fixative
for electron microscopy, J Cell Biol 35:148A). It was considered
particularly useful for fixing sperm. However, the abstract gave no
details about how to make it. A more complete description came out in
Stefanini M, De Martino C, Zamboni l, 1967, Fixation of ejaculated
spermatozoa for electron microscopy, Nature 216:173-174, which you can
check for the details.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Wednesday, February 20, 2002 8:45 AM -0500 kathy lowe {kjl226-at-vt.edu}
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I think I have it this time..
} Could someone give me information on the fixative Zamboni's solution? I
} need to know how it's made when and why it is used over another fixative.
}
} Thank you.
} Kathy







From daemon Wed Feb 20 09:14:18 2002



From: Willis.Robert-at-epamail.epa.gov
Date: Wed, 20 Feb 2002 10:05:50 -0500
Subject: SEM carbon film deposition

Contents Retrieved from Microscopy Listserver Archives
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I have a need to coat 37-mm teflon filters reproducibly, with a uniform,
thin (few hundred angstroms) carbon film. The primary objective is not
SEM analysis, but to achieve the most uniform coating over the entire
filter area. I am hoping that someone more knowledgeable and more
experienced can suggest the best type of coater for this purpose. Would
one expect a difference in uniformity or reproducibility using carbon
yarn vs. carbon rod vs. carbon sputter electrode?

Thank you for all suggestions.

******************************************************
Robert Willis, Ph.D.
Principal Scientist
ManTech Environmental Technology, Inc.
P.O. Box 12313
Research Triangle Park, NC 27709-2313
Tel: 919-541-2809 Fax: 919-541-3566
willis.robert-at-epa.gov
******************************************************





From daemon Wed Feb 20 10:09:23 2002



From: Stuart Kearns :      Stuart.Kearns-at-bristol.ac.uk
Date: Wed, 20 Feb 2002 16:00:57 +0000
Subject: position - EPMA

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Dear Listers,

Please could you bring the following job advert to the attention of
potentially interested people,

Thanks in advance,

Stu

------------------------------------------------------------------------------------
Electron Microbeam Laboratories - Support Assistant

The Department of Earth Sciences, Bristol UK, is seeking a support
assistant to work in its electron microbeam laboratories. The post is a
fixed term 12-month appointment. The department is re-equipping this
analytical area as part of a recent award. The appointee will assist in
the demanding role of maintaining a fully operational range of
analytical services whilst assisting with the procurement, installation
and commissioning of new equipment in new purpose built laboratories.


The primary role of the successful applicant will be to maintain
equipment, and to train and supervise researchers and students in all
aspects of electron microprobe and scanning electron microscope
techniques. Additionally the post affords the opportunity to become
familiar with modern state-of-the-art equipment during
installation.

The electron microbeam laboratories form one of several analytical
facilities within the EU Large Scale Geochemical Facility in the
department of Earth Sciences. Scientists from European countries visit
Bristol for periods of typically one to two weeks to collect data from
a variety of instruments on a vast range of research topics. It is
expected that the appointee will oversee some of this external work.

The successful applicant will have a background in an Earth Science
discipline and experience in wavelength dispersive electron microprobe
analysis. She/He will also possess excellent communications skills.
Further experience of SEM techniques and sample preparation would be an
advantage.

Approximate start date - July 2002. Applications by CV, covering letter
and names and addresses of three referees to Dr Stuart Kearns at the
address below by 9am, 15th March 2002. For informal enquiries and
further particulars, please contact Dr. S.L.Kearns
(stuart.kearns-at-bristol.ac.uk) Dept. Earth Sciences, Queens
Road, University of Bristol, Bristol, UK, BS8 1RJ
-------------------------------------------------------------------
Stuart Kearns
Electron Microbeam Laboratories
Department of Earth Sciences
University of Bristol
Queens Road
Bristol BS8 1RJ
UK
tel: (0)117 954 5435
fax: (0)117 925 3385
e-mail: Stuart.Kearns-at-bristol.ac.uk
http://www.gly.bris.ac.uk
http://eugf.gly.bris.ac.uk
--------------------------------------------




From daemon Wed Feb 20 11:52:11 2002



From: Xudong Fan :      fanx-at-msu.edu
Date: Wed, 20 Feb 2002 12:48:31 -0500
Subject: Re: Questions on the Electron Microscope

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Ken,

You don't know how much I truly appreciate your time and answer(also to Gary).
Since I am not a OLM person, much of the knowledge relates to that is coming
from textbook and misconception, especially for Q1.

Ken Tiekotter wrote:

} *You must specify TEM or SEM or STEM or ESEM. You cannot ask these
} questions as to how they relate solely to "EM", as the answers relating to
} physical design and the physics associated with their operation are unique
} to each instrument.
}
} You state "better depth of field/focus". Yes, in the SEM the scanning
} probe instruments, depth of field/focus is important. However, depth of
} field in a 90nm section for TEM is, as you can imagine, extremely small,
} hence the advantage of accelerating voltage.

Sorry, I simplified too much. However, TEM does have large depth of FOCUS.

} As for the disadvantages of EM as being labor intensive, have you any
} knowledge of LM preparation or the difference between TEM and SEM
} preparation? SEM preparation is a walk in the park compared to the rigors
} necessary to preserve, examine, and document ultrastrutural resolution at
} 0.1nm.
}
} ... much deleted...
}
} As for instrument expense, look into a multi-photon, scanning laser
} confocal OLM microscope at $400k to $600K!

I did not know OLM can cost that much and how labor intensive it can be.
Please excuse my ignorance. I believe my answer is from some textbook.
Shouldn't this be the very reason that the student should ask YOUR
opinion rather than just the textbook?

} } If I ask all the light microscopist, how many of your tasks can be
} } simply replaced with electron microscopy - presume you have enough
} } funding and man power?
}
} Toolbox...more expensive toys do not necessarily answer the simplest
} questions

Excellent analogy. That is why I think Q1 maybe misleading. You can not
really compare the advantage and disadvantage directly. Most of the task
by OLM can not replaced by EM, or vice versa. Just like you can not ask
"what is the advantage to use a power scroll driver other than a hammer?".
However, as I suggested earlier, you may list the general advantage/disadvantage
of each individual tools in the toolbox.


} Do you not agree there is a limit to the resolution of the OLM regardless
} of wavelength or with respect to wavelength? If the OLM resolution were
} limitless with respect to wavelength, why bother with the TEM?

Thank you to point that out. I don't know exactly what you meant on the limitation

of OLM, but one thing I can think of is the X-ray microscopy(if you still consider

X-ray is a light). Whatever your reason is, not only we should "bother" with TEM,
but also we should "bother" with "OLM".
If you are seeking for a simpler answer to Q2, it could be "generally the shorter
the wavelength
the better the microscope resolution. However it may not be true when the
wavelength is too short". Wouldn't what make the answer better?

} } ... much deleted ...

I have learned so much from Q1 and Q2. For Q3, I think I know generally
what "temporal resolution". But I am still seeking answers to what is means
in EM. Thanks to Gary, I start to understand, but not good enough to
teach another person. If no one answers, I can do some more research myself.

Finally, I have some general comment:
I believe there are three groups of people when facing a certain question :

First group is those who don't know the answer.
Second group is those who are not sure about the answer
Third group is those who know the correct answer.
Of course there is another group who doesn't care about the answer.

In the first group. there are who don't admit, or unwilling to ask, (e.g.
hopefully none)
and who are not afraid to admit and willing to ask(e.g. the student and hopefully
meself)
In the second group, there are who don't wish to discuss and those who wish to
discuss and seeking the right answer(e.g. us)
In the third group, there are who are not willing to teach and who are willing to
teach(e.g. Ken, Gary, and many more out there...)

So thank you so much to those who are interested in these discussions. I hope
you learn as much as I do.

Yours sincerely,
Xudong

*********************************************************************
Confucius Says:
If you know, you do.
If you don't know, you don't.
Don't be ashamed to ask.
*********************************************************************



From daemon Wed Feb 20 11:57:57 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 20 Feb 2002 12:51:29 -0500
Subject: Re: Questions on the Electron Microscope

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
In relation to this, resolution claims by original equipment manufacturers for SEMs are usually based on images of a gold on carbon sample. This is an "ideal" sample. However, few of us have ideal samples. Does anyone have information on realistic resolutions to expect from a FEG-SEM as compared with a standard SEM with tungsten filament for the following sample types?

a) dry, coated biological(or low density polymer) sample - low/medium topography
b) hydrated sample such as polymer or collagen gels prepared using cryo-SEM
c) hydrated nanotubes or vesicles using cryo-SEM
d) pure metal samples looking for grain boundaries- uncoated

I understand that FEG should give 3-5 times better resolution at low kV than standard gun but do not know how to relate that to real life samples. Information such as working distance and kV used as well as magnificaitons when determining the resolution would be of interest. Any reasonable guess would be appreciated.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



On Wednesday, February 20, 2002 3:33 AM, Chris Jeffree {c.jeffree-at-ed.ac.uk} wrote:
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From daemon Wed Feb 20 12:06:33 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 20 Feb 2002 10:04:29 -0800
Subject: Re: Questions on the Electron Microscope

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At 09:48 AM 2/20/2002, you wrote:

} I have learned so much from Q1 and Q2. For Q3, I think I know generally
} what "temporal resolution". But I am still seeking answers to what is means
} in EM. Thanks to Gary, I start to understand, but not good enough to
} teach another person. If no one answers, I can do some more research myself.

Temporal has to do with time. Thus, temporal resolution is
the ability to resolve or capture an image when the specimen
is moving. With LM, a video camera can do this as
would multiple snaps with a film camera. The reason
this works is that the image is immediately captured and
thus, specimen motion is less of an issue (all other things
being equal of course).

For SEM, temporal resolution is a problem. Imagine a
micromachine moving at say 20 Hz. And we want to capture
that movement in the SEM at 5KX. How long does it
take to slow scan a frame? Shortest on my system is
about 30 Seconds. So the object is moving at a 50mS
rate but I can only capture one image every 30 Seconds.
Bad temporal resolution. In slow scan mode, even the
fastest of the slow scan rates, I can't see the machine
move. Under LM, I could, if I could get 5KX mag. Perhaps
a confocal would do this. But irrespective, this is the basic
idea.

A solution? Don't have the machine continuously move.
Step it in discrete amounts and capture each position.
Then, put them all together into an avi or Quicktime file.

Another option is to video record the RS-170 out of
my SEM in partial field. This is a faster scan rate
which is buffered and output as a high rez RS-170 field.
Other systems can likely do this too.

gary g.



From daemon Wed Feb 20 15:26:10 2002



From: sghoshro-at-NMSU.Edu
Date: Wed, 20 Feb 2002 14:17:34 -0700 (MST)
Subject: pollen viability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everybody,

A graduate student is interested in looking at viability of freshly
collected pollens from his research plants. Is there a quick method to do
this instead of looking at germination ? Is there any vital stain or other
dyes available ? We do have access to epiflourescence microscope.

Thanks in advance.

Soumitra

*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml



From daemon Wed Feb 20 15:26:10 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 20 Feb 2002 13:18:04 -0800
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've seen Gold on Carbon and Tin on Carbon samples. Even
these vary from specimen to specimen, how they were stored,
how long they were stored, etc.

The main advantage of FESEM is brightness, in my view.
Arguably, the Schottky FE is more stable than a cold FE.
Be that as it may, brightness is a given I'm sure. Resolution
is, I believe, based on three factors: 1) specimen contrast,
2) electron optics performance, and 3) sample volume
limitations.

Number 1 is why the makers like Gold on Carbon--
huge contrast. Contrast values from 1 to 1E-3 can have
related probe sizes from 25A to 25,000A.

The electron optics are really critical. Just their basic
inherent design. For example, I work with two FESEMs,
both of which use the same Schottky FE gun. From the
bottom of the gun assembly on down the column, all
else is quite different. One SEM uses a conical lens
design while the other is a flat lens design. For the same
magnification and WD, the conical lens SEM will at 2KV
produce basically the same quality image as the flat lens
SEM does at 10KV. On a really good day, maybe at 5-6KV.
The final aperture in the conical system is 70u while a 100u
aperture is used in the flat lens system. I doubt that this
makes much difference overall.

I don't feel qualified to discuss sample volume limitations.
Perhaps others can jump in on this facet.

Based on your specimens, it seems that the first limiting
factor is specimen contrast. When one thinks that the
machine is faulty, it may actually be the specimen
which is limiting the resolution.

BTW, both FESEMs are tested with Gold on Carbon
for resolution. Conical is done at 5KV, 70u, 4mm, 200KX.
Flat guy is done at 10KV, 100u, 4mm, 200KX. Pretty
much the same results between the two. The killer for
astigmatism turns out to be the apertures (dirty) while
overall poor resolution (for good specimens) is a dirty
scan coil liner.

gary



At 09:51 AM 2/20/2002, you wrote:
} Listers,
} In relation to this, resolution claims by original equipment
} manufacturers for SEMs are usually based on images of a gold on carbon
} sample. This is an "ideal" sample. However, few of us have ideal
} samples. Does anyone have information on realistic resolutions to expect
} from a FEG-SEM as compared with a standard SEM with tungsten filament for
} the following sample types?
}
} a) dry, coated biological(or low density polymer) sample - low/medium
} topography
} b) hydrated sample such as polymer or collagen gels prepared using cryo-SEM
} c) hydrated nanotubes or vesicles using cryo-SEM
} d) pure metal samples looking for grain boundaries- uncoated
}
} I understand that FEG should give 3-5 times better resolution at low kV
} than standard gun but do not know how to relate that to real life
} samples. Information such as working distance and kV used as well as
} magnificaitons when determining the resolution would be of interest. Any
} reasonable guess would be appreciated.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail:
} dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907



From daemon Wed Feb 20 15:27:06 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 20 Feb 2002 13:25:38 -0800
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:32 AM 2/20/2002, you wrote:


} Gary Gaugler wrote:
}
} } The URLs are not the answer. What is at the URLs is. You have
} } to read the material at the URLs to compose an answer for yourself.
} } Better still, look up the dictionary definition of "temporal" and
} } then read the material at the URLs. If you still don't get it,
} } let me know.
}
} I don't think I have probelm understand the meaning of "temporal
} resolution" literally. If I am right, our limited eye "temporal resolution"
} allow us to see fast changing stationary pictures(24 frames/sec) as Hollywood
} movies.
} Sure I have to read the content on URLs. But the content does not provide a
} direct answer. Now I can short of figure out what it means with SEM.
} Is that means how well you can see a moving sample(MEMS)
} vibrating at high frequency in a SEM?

[snip]

I think you have reached the point where one should
be able to put the pieces together in a coherent, concise
form and answer all of the questions. And in addition,
be able to discuss the nuances of LM, SEM and TEM
in general and with specifics.

Given the number and types of factors surrounding the
questions, and those already disclosed, some on-line
research and bookwork should do the job nicely.
I'm pretty sure it would. Don't you think so?

gary g.



From daemon Wed Feb 20 15:41:41 2002



From: Xudong Fan :      fanx-at-msu.edu
Date: Wed, 20 Feb 2002 16:40:27 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just before you send this, I think I figured out myself too. I am a TEM person,
so the concept of observing moving object never come to me. Even so, TEM never
fail to capture any move. So I figure out it must be something to do with the
scan system. Does the fastest scan rate(TV rate ~ 50Hz) has anything to do
with the temporal resolution limit of 50-100Hz in SEM?

I don't think there is any meaning to talk about "temporal resolution" in
stationary
beam microscopes. Should Q3 be better asked as "How is the temporal resolution
produced in a scanning microcope(including scanning light microscope)?" rather
than "electron microscope"?

By the way, what is the official unit for "temporal resolution?" Hz? or sec?
Well, thank you so much.

Gary Gaugler wrote:

} At 09:48 AM 2/20/2002, you wrote:
}
} } I have learned so much from Q1 and Q2. For Q3, I think I know generally
} } what "temporal resolution". But I am still seeking answers to what is means
} } in EM. Thanks to Gary, I start to understand, but not good enough to
} } teach another person. If no one answers, I can do some more research myself.
}
} Temporal has to do with time. Thus, temporal resolution is
} the ability to resolve or capture an image when the specimen
} is moving. With LM, a video camera can do this as
} would multiple snaps with a film camera. The reason
} this works is that the image is immediately captured and
} thus, specimen motion is less of an issue (all other things
} being equal of course).
}
} For SEM, temporal resolution is a problem. Imagine a
} micromachine moving at say 20 Hz. And we want to capture
} that movement in the SEM at 5KX. How long does it
} take to slow scan a frame? Shortest on my system is
} about 30 Seconds. So the object is moving at a 50mS
} rate but I can only capture one image every 30 Seconds.
} Bad temporal resolution. In slow scan mode, even the
} fastest of the slow scan rates, I can't see the machine
} move. Under LM, I could, if I could get 5KX mag. Perhaps
} a confocal would do this. But irrespective, this is the basic
} idea.
}
} A solution? Don't have the machine continuously move.
} Step it in discrete amounts and capture each position.
} Then, put them all together into an avi or Quicktime file.
}
} Another option is to video record the RS-170 out of
} my SEM in partial field. This is a faster scan rate
} which is buffered and output as a high rez RS-170 field.
} Other systems can likely do this too.
}
} gary g.



From daemon Wed Feb 20 16:12:54 2002



From: Xudong Fan :      fanx-at-msu.edu
Date: Wed, 20 Feb 2002 17:10:57 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Gary Gaugler wrote:

} I think you have reached the point where one should
} be able to put the pieces together in a coherent, concise
} form and answer all of the questions. And in addition,
} be able to discuss the nuances of LM, SEM and TEM
} in general and with specifics.
}
} Given the number and types of factors surrounding the
} questions, and those already disclosed, some on-line
} research and bookwork should do the job nicely.
} I'm pretty sure it would. Don't you think so?
}
} gary g.

Yes, I am glad I did - BEFORE you posted the right answer.
I am also VERY glad that you did not tell me the straight
answer at the beginning. You are a great teacher.
However, if I am going to teach another person about this,
I will correct the question first.



From daemon Wed Feb 20 17:17:04 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 21 Feb 2002 12:07:46 GMT+1200
Subject: quant analysis of Fe oxides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi

I sent this, in a slightly different form, to the microprobe server,
but it doesn't seem to have been broadcast, so sorry to those who
receive it twice and to those who aren't interested in this sort of
thing.

My EDS quantitation software, like most, I think, allows a choice of
valency for Fe expressed as oxide, so that it comes out as either FeO
or as Fe2O3.

We habitually choose to express it as FeO, and subsequently use one
of the several methods available to recalculate some of the Fe as
Fe2O3 as necessary, however, it's not the recalculation that I
invite discussion on at this stage.

My question is this:

If I had a perfect, stoichiometric, 100% pure magnetite, Fe3O4,
(which formula corresponds to 72.36% Fe, 27.64% O), and analysed it,
expressing the result as FeO (which formula corresponds to 77.73% Fe,
22.27% O), what should the result be?

It's easy enough to say OK, the magnetite has 72.36% Fe, and if we
express 72.36% Fe as FeO, it's simply 72.36 divided by 0.7773, which
is 93.09.

So perfect magnetite should come out as 93.09% FeO.

But is this correct, or are there a few unjustifiable assumptions
included?

The reason I want to know is that I'd like to start using a magnetite
as the calibration standard for Fe for users to analyse
titanomagnetites. They are accustomed to the Fe in their
titanomagnetites being expressed as FeO.

I have an Astimex standard magnetite which is stated to contain
31.03%FeO, 68.76%Fe2O3, and 0.20%Cr2O3.

So what should I take as its reference value if all the Fe is
expressed as FeO?

All opinions welcome.


cheers

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed Feb 20 17:22:56 2002



From: Greg Strout :      gstrout-at-ou.edu
Date: Wed, 20 Feb 2002 17:16:51 -0600
Subject: Re: pollen viability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might try looking at the following:

Heslop-Harrison J & Y . 1970. Evaluation of pollen viability by enzymatically
induced fluorescence; intracellular hydrolysis of fluorescein diacetate. Stain

Tech 45:115-20

Heslop-Harrison J, Heslop-Harrison Y, Shivanna KR. 1984. The evaluation of
pollen quality; and a further appraisal of the fluorochromatic (FCR) test
procedure. Theor Appl Genet 67:367-75


--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================



"sghoshro-at-NMSU.Edu"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi everybody,
}
} A graduate student is interested in looking at viability of freshly
} collected pollens from his research plants. Is there a quick method to do
} this instead of looking at germination ? Is there any vital stain or other
} dyes available ? We do have access to epiflourescence microscope.
}
} Thanks in advance.
}
} Soumitra
}
} *************************************************************
} Soumitra Ghoshroy Ph.D.
} Director, Electron Microscopy Lab
} Graduate Faculty, Department of Biology
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3268 (office), 646-3283 (lab)
} Fax: 505-646-3282
} e-mail:sghoshro-at-nmsu.edu
} URL:http://confocal.nmsu.edu/eml





From daemon Thu Feb 21 02:51:19 2002



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 21 Feb 2002 00:36:09 -0800 (PST)
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


AMEN!
Ken
--
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

On Wed, 20 Feb 2002, Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} At 11:32 AM 2/20/2002, you wrote:
}
}
} } Gary Gaugler wrote:
} }
} } } The URLs are not the answer. What is at the URLs is. You have
} } } to read the material at the URLs to compose an answer for yourself.
} } } Better still, look up the dictionary definition of "temporal" and
} } } then read the material at the URLs. If you still don't get it,
} } } let me know.
} }
} } I don't think I have probelm understand the meaning of "temporal
} } resolution" literally. If I am right, our limited eye "temporal resolution"
} } allow us to see fast changing stationary pictures(24 frames/sec) as Hollywood
} } movies.
} } Sure I have to read the content on URLs. But the content does not provide a
} } direct answer. Now I can short of figure out what it means with SEM.
} } Is that means how well you can see a moving sample(MEMS)
} } vibrating at high frequency in a SEM?
}
} [snip]
}
} I think you have reached the point where one should
} be able to put the pieces together in a coherent, concise
} form and answer all of the questions. And in addition,
} be able to discuss the nuances of LM, SEM and TEM
} in general and with specifics.
}
} Given the number and types of factors surrounding the
} questions, and those already disclosed, some on-line
} research and bookwork should do the job nicely.
} I'm pretty sure it would. Don't you think so?
}
} gary g.
}
}





From daemon Thu Feb 21 03:27:09 2002



From: Ian MacLaren :      maclaren-at-hrem.mpi-stuttgart.mpg.de
Date: Thu, 21 Feb 2002 10:20:52 +0100
Subject: Materials-l

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Does anyone know what became of the materials-l email discussion group that
was based at Liverpool (UK). I tried logging on yesterday and got an error
message saying that the address listproc-at-liv.ac.uk didn't exist.

Thanks

Ian MacLaren
MPI für Metallforschung, Heisenbergstr. 3, 70569 Stuttgart, Germany
http://www.mpi-stuttgart.mpg.de/

ian.maclaren-at-physics.org / http://members.tripod.co.uk/IanMacLaren/




From daemon Thu Feb 21 04:14:22 2002



From: N.Grobert :      nicole-at-mf.mpg.de
Date: Thu, 21 Feb 2002 11:06:36 +0100 (MET)
Subject: EM studies of nanotubes and novel composite materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EC TMR-Network NANOCOMP (1 POST- and 1 DOCTORAL Research Position)


"Large Scale Synthesis of Carbon Nanotubes and their Composite Materials"
Ten European Partners from B, CH, D, ES, F, HUN, and IR built the NANOCOMP
Network. NANOCOMP started in September 2000. The objectives of the network
are:

- Production of carbon nanotubes: Process-optimization
- Control of nanotube characteristics
- Purification
- Preparation of composite materials
- Multidisciplinary characterization

As part of the NANOCOMP programm

1 POST- and 1 DOCTORAL Research Position

are available (start date: immediately) at the Max-Planck-Institut fr
Metallforschung in Stuttgart, Germany

Within the network the Stuttgart research team is responsible for the
Microstructural Characterization of Carbon Nanotubes and
Carbon Nanotube-based Composites using

* High resolution transmission electron microsocopy,
* High spatial resolution electron energy-loss spectroscopy and EDX and
* other characterization tools (SEM, AFM, STM, XPS, etc.)
The department of Prof. M. Rhle at the MPI fr Metallforschung in Stuttgart
offers exceptional transmission electron microscopy instrumentation and
expertise.

Requirements:
* Doctoral degree (for PostDoc)
* National of a member state of the EU or an Associated State
* Background in physics, chemistry or materials science


The aim of the group at the MPI fr Metallforschung is to develop new
methods for the controlled sythesis of carbon nanotubes and related
structures. These materials will be employed for novel composite materials
exhibiting interesting chemical and physical properties.
As a member of the NANOCOMP network you will have the exciting chance to
perform research in close European collaboration with Postdocs and PhD
students of other leading European teams including regular network
meetings, research stays at partner groups and the participation at
European conferences.

NANOCOMP Partners are (in alphabetical order):

1. Consejo Superior de Investigaciones cientificas, Instituto de
Carboquimica, Zaragoza, Spain
2. Department of Political Science, Sdertrns Hgskola Stockholm, Sweden
3. Ecole de Mines de Paris, Centre d'Energtique, Sophia Antipolis, France
4. Facults Universitaires Notre-Dame de la Paix, Dpartement de Chimie,
Namur, Belgium
5. Research Institute for Technical Physics and Materials Science,
Laboratory for Nanostructure Research Budapest Hungary
6. Suiss Federal Institute of Technology, Institut de Gnie atomique,
Lausanne, Switzerland
7. University of Dublin, Trinity College, Dublin, Ireland
8. Universit de Lige, Laboratoire de Gnie Chimique, Lige, Belgium
9. Jozsef Attila University, Applied and Environmental Chemistry, Szeged,
Hungary
10. Universit d'Orlans - CNRS, Centre de Recherche sur la Matire Divise,
Orlans, France

Contact (Information and Application)

Prof. Dr. Manfred Rhle
Phone: +49-711-689-3520
Ruehle-at-mf.mpg.de

Dr. Nicole Grobert
Phone: +49-711-689-3603
Grobert-at-mf.mpg.de

MPI fr Metallforschung, Heisenbergstrasse 3, D-70569 Stuttgart, Germany
Fax.: +49-711-689-3522

February 2002





From daemon Thu Feb 21 05:54:58 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Wed, 20 Feb 2002 07:45:29 -0500
Subject: Printing images from Philips 515 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I wish to digitally save and print images from a Philips 515 SEM.
Is there some way to get an output from this SEM that can be saved as a Bitmap or TIFF
file, or otherwise digitized and printed?






From daemon Thu Feb 21 08:50:13 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 21 Feb 2002 09:26:31 -0500
Subject: quant analysis of Fe oxides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm reaching back here, but isn't magnetite a mix of Fe+2 and Fe+3 and you should get both FeO and Fe2O3?

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz]
Sent: Thursday, February 21, 2002 7:08 AM
To: Microscopy-at-sparc5.microscopy.com



Hi

I sent this, in a slightly different form, to the microprobe server,
but it doesn't seem to have been broadcast, so sorry to those who
receive it twice and to those who aren't interested in this sort of
thing.

My EDS quantitation software, like most, I think, allows a choice of
valency for Fe expressed as oxide, so that it comes out as either FeO
or as Fe2O3.

We habitually choose to express it as FeO, and subsequently use one
of the several methods available to recalculate some of the Fe as
Fe2O3 as necessary, however, it's not the recalculation that I
invite discussion on at this stage.

My question is this:

If I had a perfect, stoichiometric, 100% pure magnetite, Fe3O4,
(which formula corresponds to 72.36% Fe, 27.64% O), and analysed it,
expressing the result as FeO (which formula corresponds to 77.73% Fe,
22.27% O), what should the result be?

It's easy enough to say OK, the magnetite has 72.36% Fe, and if we
express 72.36% Fe as FeO, it's simply 72.36 divided by 0.7773, which
is 93.09.

So perfect magnetite should come out as 93.09% FeO.

But is this correct, or are there a few unjustifiable assumptions
included?

The reason I want to know is that I'd like to start using a magnetite
as the calibration standard for Fe for users to analyse
titanomagnetites. They are accustomed to the Fe in their
titanomagnetites being expressed as FeO.

I have an Astimex standard magnetite which is stated to contain
31.03%FeO, 68.76%Fe2O3, and 0.20%Cr2O3.

So what should I take as its reference value if all the Fe is
expressed as FeO?

All opinions welcome.


cheers

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Feb 21 09:47:36 2002



From: Xudong Fan :      fanx-at-msu.edu
Date: Thu, 21 Feb 2002 10:46:09 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Michael O'Keefe wrote:

} } By the way, what is the official unit for "temporal resolution?" Hz? or sec?
} Since Hz is frequency, temporal resolution must be sec (or msec)...

Okay, this helps me understand better. This means that the temporal resolution is
not just
for vibrating object. Resolution of 1sec means you can not resolve vibration higher
than 1Hz, No problem.
What does this 1sec mean if you wish to observe an object which is moving in one
direction?
My definition: you can only capture the motion that is taking more than 1sec to
across the
screen. For example, your scan rate must be longer than 1sec/frame, you only can
capture one
position of the object, the following frames catches nothing. So the motion is not
resolved.
However, if I keep this scan rate, and simply reduce the magnification, say by 10X,
now
I can capture the motion by capture 10 positions of the object. Now the same motion
is resolved.
Was the temporal resolution improved? I guess not.



From daemon Thu Feb 21 09:47:36 2002



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Thu, 21 Feb 2002 09:40:47 -0600
Subject: pollen viability (an answer)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Soumitra,
Yes, there is a quick and easy way. I don't have my original protocol, but
this one will probably do (from Ruzin's Plant Microtechnique and
Microscopy). Dissolve 1 mg of fluorescein diacetate (FDA) in 1 mL of
acetone for a stock solution that can be stored at -20C. Then add to pollen
grains for a grand total concentration of 1 microgram FDA/mL and incubate
for 5 minutes. Observe under blue light (488 nm). Living cells will
fluoresce yellow/green. My pollen was usually in germination medium at the
time so that is into what I added the FDA. I'll cross-check this with my
old files and let you know if there are any significant differences. Good
luck.
Kristen



Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
515-294-8854
kalen-at-iastate.edu
www.baumlab.org



From daemon Thu Feb 21 10:59:29 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 21 Feb 2002 08:52:17 -0800
Subject: Re: Questions on the Electron Microscope

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Dear Debby,
I was just using a new cold-FESEM at another department at our university
and comparing the results with the photos from our ten-year-old, W-filament
SEM on coated ceramic samples with tiny pores. The results were that we
could take photos at 200KX on the FESEM with the same clarity as 20KX on our
older SEM. They haven't used the cryo much yet, but the resolution seems to
be about ten times. We could easily resolve the thin gold-palladium coating.
We tried a little 5 Kv work on the FESEM and we could still get a good
picture at 150KX, so I think resolution is at least five to ten times that
of a W-filament SEM.
At 12:51 PM 2/20/02 -0500, you wrote:
}
} Listers,
} In relation to this, resolution claims by original equipment
manufacturers for SEMs are usually based on images of a gold on carbon
sample. This is an "ideal" sample. However, few of us have ideal samples.
Does anyone have information on realistic resolutions to expect from a
FEG-SEM as compared with a standard SEM with tungsten filament for the
following sample types?
}
} a) dry, coated biological(or low density polymer) sample - low/medium
topography
} b) hydrated sample such as polymer or collagen gels prepared using cryo-SEM
} c) hydrated nanotubes or vesicles using cryo-SEM
} d) pure metal samples looking for grain boundaries- uncoated
}
} I understand that FEG should give 3-5 times better resolution at low kV
than standard gun but do not know how to relate that to real life samples.
Information such as working distance and kV used as well as magnificaitons
when determining the resolution would be of interest. Any reasonable guess
would be appreciated.
} Debby

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Thu Feb 21 12:13:59 2002



From: Kathy Smith :      kasmith11435-at-yahoo.com
Date: Thu, 21 Feb 2002 10:06:11 -0800 (PST)
Subject: TEM Services

Contents Retrieved from Microscopy Listserver Archives
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To All:

We are looking for an independent TEM service
provider here along the East Coast: Does anyone have
any information?

Thank you very much.

Kathy Smith

__________________________________________________
Do You Yahoo!?
Yahoo! Sports - Coverage of the 2002 Olympic Games
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From daemon Thu Feb 21 13:03:09 2002



From: Jim Quinn :      jquinn-at-www.matscieng.sunysb.edu
Date: Thu, 21 Feb 2002 13:52:59 -0500
Subject: materials listserv

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Ian -

Below is the info you requested.

JQuinn

} From alan.thew-at-liverpool.ac.uk Thu Feb 21 13:48:29 2002
} Date: Thu, 21 Feb 2002 18:51:45 +0000 (GMT Standard Time)
} From: Alan Thew {Alan.Thew-at-liverpool.ac.uk}
} Reply-To: U of Liverpool List Admin {list.admin-at-liverpool.ac.uk}
} To: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
} cc: U of Liverpool List Admin {list.admin-at-liverpool.ac.uk}
} Subject: Re: is materials-l down?
}
} On Thu, 21 Feb 2002, Jim Quinn wrote:
}
} }
} } Alan -
} }
} } Is "material-l" down on your listserv?
}
} The right name is
}
} materials-at-liverpool.ac.uk
}
} but
}
} materials-l-at-liverpool.ac.uk
}
} still works... my test message just went out...
} }
} } thanks and regards,
} }
} } JQuinn
} }
} Alan Thew
}

} From owner-MATERIALS-at-LISTSERV.LIV.AC.UK Thu Feb 21 13:49:23 2002
} Date: Thu, 21 Feb 2002 18:52:31 +0000
} From: "L-Soft list server at U of Liverpool (1.8d)"
} {LISTSERV-at-liverpool.ac.uk}
} Subject: Information about the MATERIALS list
} To: jquinn-at-www.matscieng.sunysb.edu
} X-LSV-ListID: MATERIALS
}
} There is no information file for the MATERIALS list. Here is a copy of
} the list "header," which usually contains a short description of the
} purpose of the list, although its main purpose is to define various list
} configuration options, also called "keywords." If you have any question
} about the MATERIALS list, write to the list owners at the generic
} address:
}
} MATERIALS-request-at-LISTSERV.LIV.AC.UK
}
} *
} * Materials Science and Engineering list
} *
} * Review= owner Subscription= open,confirm
} * Send= private
} * Notify= Yes Reply-to= List,Respect Files= No
} * Validate= Yes
} * Mail-Via= Direct
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} * Sizelim= 1000
} * Attachments = No
} * Language = NOHTML
} *
} * Notebook= Yes,/listserv/notebooks/m/materials,Monthly,public
} *
} * {3 lines hidden}
} *
}


From daemon Thu Feb 21 14:44:09 2002



From: Kathy Smith :      kasmith11435-at-yahoo.com
Date: Thu, 21 Feb 2002 12:36:45 -0800 (PST)
Subject: TEM Services - Revised Question

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Sorry, I realize I should be more specific. We are
looking for an independent service provider that can
service the actual microscope. We are having issues
with our microscopes and wanted to know if anyone used
an independent service provider for this kind of work,
or if anyone knows of someone that fix microscopes.

Any info would be greatly appreciated.

Thanks,
Kathy

__________________________________________________
Do You Yahoo!?
Yahoo! Sports - Coverage of the 2002 Olympic Games
http://sports.yahoo.com


From daemon Thu Feb 21 15:58:10 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 21 Feb 2002 15:45:01 -0600
Subject: RE: Questions on the Electron Microscope

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} I understand that FEG should give 3-5 times better
} resolution at low kV
} than standard gun but do not know how to relate that to real
} life samples.
} Information such as working distance and kV used as well as
} magnificaitons
} when determining the resolution would be of interest. Any
} reasonable guess
} would be appreciated.
} } Debby

I'll talk about dentin, which when dehydrated is mostly a
porous mineral, hydroxyapatite.

For not coated dehydrated dentin in low voltage or low vacuum
modes I can use magnifications up to 10k. For not coated
hydrated dentin in wet mode - up to 20-40k. For coated with
gold-palladium - up to 100-150k. Image of gold on carbon
standard is almost as good in wet mode as in conventional mode
at 100k. Ceramics I have observed mostly behave similar.


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy




From daemon Thu Feb 21 17:38:26 2002



From: Briem . :      briemeng-at-hotmail.com
Date: Thu, 21 Feb 2002 17:30:30 -0600
Subject: ESCA parts

Contents Retrieved from Microscopy Listserver Archives
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We have a few spare parts for an ESCA Quantum 2000. Stages, gaskets, etc.
} Please e-mail at briemeng-at-hotmail.com

_________________________________________________________________
MSN Photos is the easiest way to share and print your photos:
http://photos.msn.com/support/worldwide.aspx



From daemon Thu Feb 21 17:40:27 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 21 Feb 2002 18:31:14 -0500
Subject: Microtomist's Formulary and Guide - Reprint

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Sorry for those repetitions folks, but I think that this reprint is an
opportunity for any who are new to older, histotechnique compendia. I don't
know an Krieger, but I have purchased the book, and I use it often.

Highly recommended for personal or library.

Regards,

Fred Monson

} ----------
} From: Krieger
} Sent: Thursday, February 21, 2002 5:18 PM
} To: Monson, Frederick C.
} Subject: Re: Gray Available?
}
} BOOK #: 202473 ISBN #: 0-88275-247-2
}
} AUTHOR: GRAY
}
} TITLE: MICROTOMIST'S FORMULARY AND GUIDE
}
} PRICE: 84.50 REFRL/ARRGM TYPE: CLOTH
}
} Shipping $5.00 UPS
}
} AT PRSENT WE ARE UPDATING OUR LISTS AND PORTIONS OF LOOKUP ARE OFFLINE.
}
} THE MICROTOMIST'S FORMULARY AND GUIDE
} Peter Gray,
} 0-88275-247-2
}
} Pages: 808, Binding: Cloth,
}
} Description:
} This is a known and recognized source reference work. The book includes a
} treatise on the art of making microscopic slides from biological
} specimens,
} as well as a classified list of the formulas and techniques used in this
} art.
}
}


From daemon Thu Feb 21 18:03:22 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 21 Feb 2002 16:00:06 -0800
Subject: Nikon Coolscan 8000ED - option

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I've been hearing that the Coolscan 8000 was
pulled from distribution. Mostly (??) due to
bad LED and CCD mating.

From recent research, if you want to scan from 35mm
on up to TEM negs, look at the Minolta Dimage Scan Multi Pro.
it is a 4.8D, 4800dpi 35mm, 3200dpi MF scanner with
fluorescent lamp light source (very good). It interfaces
via Ultra SCSI or Firewire. Supposedly, only Win2K and
WinME work with it on the PC.

TriState Camera (NYC) lists this unit at $2749 vs. the
MSRP of $2999. I have not tried it but am getting itchy.

gary g.


At 11:49 AM 10/25/2001, you wrote:

} Rick,
} Many of our customers are very happy with the 8000ED.
} I have looked into putting TEM negs into it and it could be
} done by modifying one of the 120/220 film holders.
} The holders have a raised lip to keep the film in the
} channel. I believe these could be removed and the film could then
} just extend out past the scan opening. I have not been able to try
} this as demand for the scanner has been very high.
} Another excellent scanner for TEM negs is the Agfa
} T2500 Duoscan. While lower in resolution(2500dpi optical) it has
} a glassless carrier design that will enable scanning of an entire
} TEM negative. It also will scan reflective originals.
}
} George



From daemon Thu Feb 21 20:28:14 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 22 Feb 2002 15:16:43 GMT+1200
Subject: Fe Oxides II

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Sorry, in my previous posting I confused things a bit with talk of
standards, please let me attempt to rephrase the question:

Let's say you're doing mineral analyses, everything nicely
standardised, Fe being expressed all as FeO, with the oxygen not
analysed but calculated from the Fe analysis with an assumed valency
for Fe of 2.

Then you hit a pure, stoichiometric magnetite, formula Fe3O4
(72.36% Fe, 27.64% O).

Because you're expressing Fe as FeO, the result will be too
low, and will be something like:

FeO 93%

This is the 72.36 multiplied by the formula weight ratio of FeO/Fe
(71.85/55.85, ie 1.2865), and comes actually to 93.09%.

Is that all there is to it?

Is that the result that you would expect/accept?

Or does the underestimation of oxygen content which results from the
assumption of Fe valency of 2 have a significant effect on the ZAF
calculations or on some other part of the calculations
involved in the generation of the result?

I'm grateful for the replies received so far, but would you mind
posting them on the list as well as to me personally?

cheers

rtch






Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Fri Feb 22 01:39:26 2002



From: =?utf-8?Q?Krzysztof_Jan_H=C3=BCbner?= :      hubner-at-IOd.krakow.pl
Date: Fri, 22 Feb 2002 08:05:28 +0100
Subject: =?utf-8?Q?SEGREGATION=C2=B402?=

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SEGREGATION´02
1st International Conference
Košice, 6th – 7th June 2002

An International Conference marking the 50th anniversary of the
establishment of the Metallurgical Faculty at the Technical University in
Košice. This conference continues a series of occasional scientific meetings
on segregation of impurities and their effect on the applied properties of
metal materials which have previously been held at the Department of
Materials Science, Faculty of Metallurgy of the Technical University in
Košice. Segregation processes occur in the majority of steels from
crystallization through to the final technical processing into finished
products. This means that they influence material quality and applied
properties over the whole range of production technology, including casting,
welding, shaping and heat- or chemical-heat treatment. Segregation processes
also continue to affect finished products in connection with their external
working conditions.
The 1st International Conference on Segregation will focus primarily on
theoretical questions of phase interfaces and segregation processes as well
as their degradation features, in the following areas:
 identification methods of segregation processes
 structure, models and properties of phase interfaces
 dendritic segregation
 macrosegregation at solidification
 grain boundary segregation, or segregation to other phase interfaces
 interaction segregation and precipitation
 effect of segregation and precipitation on embrittlement and properties of
materials at
- casting
- welding
- forming
- heat- or chemical–heat treatment
- during exploitation
The principal aim of conference is to facilitate exchange of the latest
insights and the setting-up of personal and professional contacts.
The conference languages
Slovak, Czech and English

Conference venue
Campus of the Technical University in Košice

Call for papers
Both oral presentation and posters are welcome, proposals for which should
be submitted with a short abstract ( not exceeding 200 words).

Deadlines:

March 4, 2002 : Abstract deadline
March 15, 2002: Acceptance notification date
April 15, 2002 : Manuscript due

Information

Assoc. Prof. M. Longauerová
Technical University of Košice
Faculty of Metallurgy
Department of Materials Science
Park Komenského 11
042 00 Košice
SLOVAKIA

PHONE: +421 95 602 27 74
+421 95 602 25 40
+421 95 633 35 49
FAX: +421 95 60222 43
E–MAIL: Margita.Longauerova-at-tuke.sk





From daemon Fri Feb 22 04:10:50 2002



From: Norman Charnley :      norman-at-earth.ox.ac.uk
Date: Fri, 22 Feb 2002 09:54:51 +0000 (GMT)
Subject: Re: Fe Oxides II

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On Fri, 22 Feb 2002, Ritchie Sims wrote:

} Let's say you're doing mineral analyses, everything nicely
} standardised, Fe being expressed all as FeO, with the oxygen not
} analysed but calculated from the Fe analysis with an assumed valency
} for Fe of 2.
}
} Then you hit a pure, stoichiometric magnetite, formula Fe3O4
} (72.36% Fe, 27.64% O).
}
} Because you're expressing Fe as FeO, the result will be too
} low, and will be something like:
}
} FeO 93%
}
} This is the 72.36 multiplied by the formula weight ratio of FeO/Fe
} (71.85/55.85, ie 1.2865), and comes actually to 93.09%.
}
} Is that all there is to it?

As far as I am aware, yes. In the same way, if you measure calcite
(CaCO3), the total for oxides (CaO) will be only about 55% (ignoring other
problems!). And if you measure pure Fe2O3, then the result expressed as
FeO will be only about 90%, so that multiplying by the FeO -} Fe2O3
conversion factor (1.1113) gives 100%, expressed as Fe2O3.

} Is that the result that you would expect/accept?

Yes, given that you are then going to try and calculate what the true
oxidation state is from charge balance. When taken back through the
formula calculation that should improve your oxide total, as part of the
FeO is then expressed as Fe2O3.

} Or does the underestimation of oxygen content which results from the
} assumption of Fe valency of 2 have a significant effect on the ZAF
} calculations or on some other part of the calculations
} involved in the generation of the result?

This is a possibility, since the oxygen will have some effect upon the Fe
correction (but not as much as the other way round, if you try and measure
oxygen directly). The effect is probably not very significant - try
playing with ZAF corrections off-line to gauge how much.

This is a case where measuring oxygen by difference would be more
realistic and helpful than measuring oxygen by stoichiometry.

Cheers,

Norman

=================================================
Dr. Norman Charnley
Department of Earth Sciences
University of Oxford
Oxford OX1 3PR, UK.

+44 1865 272009/283741 (SEM/Electron Microprobe Labs)
+44 1865 282131 (Microsims Ion Probe Lab.)
==================================================



From daemon Fri Feb 22 06:57:31 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 22 Feb 2002 13:47:28 +0100
Subject: Re: Questions (about resolution) on the SEM

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Why do we all test a new SEM befor buying with our own samples ? It's
exactly why the gold of carbon sample is so far from our real needs, as
Bebby said. It's good at high voltage, but its only real advantage is that
all manufacturers use the same test, however the results may vary with the
age of the sample etc...

A nice test I do often with students, is to look at an egg shell : outher
wall, inner wall, inner membrane, etc, coated or uncoated, at different
energies, on a W-SEM and on a FE-SEM etc. It shows that in fact this
"resolution" question in the SEM is a false problem. Is the word
resolution attapted to what we speek from ? The real question is :

"What do I see, and what am I missing ? "

When peolpe speak from High Resolution SEM, they think always of FE-SEM.
With a FE source, all is not done. To perform HR, I must have of course a
FE gun, but also a design of the optic optimised for primary energy for a
few hundred eV to a few keV (high voltage is always good), and a choice of
detectors which allows to separate or mix SE and BSE.

So, the set of parameters I will choose depends of the sample and of which
probleme I have to solve. The type of source is one element among a lot :
FE or W fil i.e. more or less brightnes, current, chromatic
aberration, spot size
WD, i.e. more or less depth of field. And what does depth of field
mean, whith a 100k X magnification ? WD gives also more or less SE/BSE in
the classical ET detector.
Primary energy, i.e. more or less interaction volume, (or other
said variation in the ratio between surface and volume informations), more
less SE/BSE yield.
Detector, i.e. the choice between BSE, SE, or a mixing of the two
signals, in conjonction with the primary energy.

It's a new thing for a lot of SEM user, to have to precise which detector
has been used to perform the image they are showing. Most people are (more
or less...) familiar with the WD and kV indication, but realy less with
precision about "in lens-SE ", classical (Everhart-Thornley) or BSE
detectors. I have made a test with a interresting sample (carbon nanotubes
grown on a Co film on a Si (100) single crystal. The same location, at the
same magnification at two energies, with the different detectors avaible,
and I obtained 8 differents pictures. A closer look leaves 6 really
different pictures, with different resolutions, but with different
informations too. By the way, it would be nice if the manufacturer could
"standardise" the name of their "In Lens", "Throught the lens", "Upper",
"SE" detetcors !

And other aspect must be mentionned, which is the ability to perform
"good" resolution images of insulators, without coating. The performences
at low energy of the HR-SEM is in that case the key element, and the
resolution is not the main factor. We have performed images of organic
compounds deposed on an teflon (!) film on glass (!). In a classical SEM,
or older FE-SEM, we could not observe anything, because charging. With
gold coating, at high energy the surface information is lost, and at low
energy, the resolution is too poor. In recent HR-SEM from all brand, we
could do it, with correct resolution (crystals from 100 to 300 nm at x
100k ), but at 0.5 to 1.5 keV, SE detector, and less enought charging to
perform an image (of course without any coating).

It would be interresting to propose a set of "testing samples" of
different atomic number combinaisons, light and light, heavy and heavy,
light on heavy and vice versa, dry or hydrated, inorganic or organic,
which could everyone may himself or obtain easy, to perform
resolution/information tests at different energy, WD, detector, etc.
Perheps such a inventory soon exists ?

One could take, for exemple :
gold on carbon (to continue the tradition)
carbone nanotubes dispersed on a heavy metal (gold is expensive,
but why not a W foil)
CVD silcon can grow with nice crystallite, wiskers, in particular
when there are impurities
insulators such as Al2O3, glasses, ceramics (Chris Jeffree told me
from the inner surface from a broken halogen lamp, i.e. W crystallites
"moving" on the glass. Is it right?)
catalysers (heavy metals in ceramic powder, arc owen C nanotubes
and its catalysr) to test spatial resolution of the BSE detectors in compo
mode.
etc...

I am not biologist, so I have no idee what could be a good biological test
specimen, in dry or cryo mode.

Other idees ? Yes, certainly !


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On 20 Feb 2002, Debby Sherman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers,
} In relation to this, resolution claims by original equipment manufacturers for SEMs are usually based on images of a gold on carbon sample. This is an "ideal" sample. However, few of us have ideal samples. Does anyone have information on realistic resolutions to expect from a FEG-SEM as compared with a standard SEM with tungsten filament for the following sample types?
}
} a) dry, coated biological(or low density polymer) sample - low/medium topography
} b) hydrated sample such as polymer or collagen gels prepared using cryo-SEM
} c) hydrated nanotubes or vesicles using cryo-SEM
} d) pure metal samples looking for grain boundaries- uncoated
}
} I understand that FEG should give 3-5 times better resolution at low kV than standard gun but do not know how to relate that to real life samples. Information such as working distance and kV used as well as magnificaitons when determining the resolution would be of interest. Any reasonable guess would be appreciated.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907
}
}
}
} On Wednesday, February 20, 2002 3:33 AM, Chris Jeffree {c.jeffree-at-ed.ac.uk} wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} } -----------------------------------------------------------------------.
} }
} }
} } Shorter wavelength=greater resolution may be the general rule for all
} } imaging systems using waves, but
} } in SEMs that is far from the whole story, and no commercial TEM comes
} } anywhere close to the theoretical resolution limit. TEM resolving
} } power is spoiled by lens aberrations. I remember a recent paper which
} } described a way of neutralising spherical aberrations in a TEM using
} } an electron mirror.
} }
} } Early SEM guns and optics were designed for 30 kV, and were virtually
} } unusable at 1kV. SEM development since the 60s has been about
} } emitters, guns and optics. Now, with FEGSEMs we can see that although
} } the fundamental rules still apply, major improvements in overall
} } performance at low kV have been possible by using near-monochromatic
} } emitters. Resolution in uncoated light-element specimens is now seen
} } to be a trade-off between kV/wavelength and beam interaction volume.
} } In the surface of a potato starch grain, starch crystal edges can be
} } seen at 1 or 2kV, but these are obliterated at 5 or 10 kV as the beam
} } interaction volume grows. Resolution in TEM is also a function of
} } contrast, which is poorer at high kV, which is why some biological
} } TEMs are designed with long working distance optics, trading some
} } resolving power to buy higher contrast. EM instruments have greater
} } theoretical RESOLVING POWER at higher kV, but in practice the
} } RESOLUTION can be poorer. Resolving power is an instrument property.
} } Resolution can be a specimen or image property. The two do not always
} } coincide.
} }
} } Chris
} }
} } } Let's see. In SEM, resolution is inversely proportional to
} } wavelength
} } } of the electrons. Shorter wavelength, greater resolution, in
} } general.
} } } Wavelength of electrons is inversely proportional to energy. Higher
} } } energy, shorter wavelength. So, higher voltage/energy, higher
} } } resolution (more resolving power). My old SX-40 brochure says
} } } that it had a resolution of 60A. Newer SEMs are better than that.
} } } My SEM is newer than the SX-40 and is supposed to have a
} } } resolution of about 40A. I guess so, but I can't measure it.
} } } I tried but failed. I get about 120-180A at 15KV. And that's
} } } an eyeball guess. I cannot find a perfectly sharp edge!
} }
} }
} }
}
}



From daemon Fri Feb 22 09:21:13 2002



From: Curtis Olson :      COlson-at-scpglobal.com
Date: Fri, 22 Feb 2002 08:09:21 -0700
Subject: SEM Service Lab to image integrated circuits

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I am looking for SEM services in the Western USA that have high resolution (FE) SEM capabilities (and experience) of integrated circuit samples. Planar, oblique, and cross sections of IC components would be necessary.

We generally have a relatively low volume of samples. Does anyone know of a resource directory of services such as this? Any suggestion would be appreciated.

Thank you

Curtis Olson



From daemon Fri Feb 22 09:25:27 2002



From: Xudong Fan :      fanx-at-msu.edu
Date: Fri, 22 Feb 2002 10:23:29 -0500
Subject: Re: Questions on the Electron Microscope

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I agree.
So even I can take 10 positions of the object to capture the move, I still have no
idea how it moves BETWEEN each of these 10 step. Therefore the temporal
resolution does not change. The motion is captured at a LOWER resolution.
I may be able to intropolate the moving by fitting a curve of these 10 positions.
But that's not what machine tells us. In another word, it's our own judgement
beyoung machine resolution.

Now here is another question.
For a vibrating object, reduce magnification obviously won't catch the motion.
But by blanking the beam periodically(as suggested by Gary and
NASA JPL lab), a crips image of the vibrating object can be resolved. Or by adjusting
the blanking frequency, it can be seen in a slow motion.
Does that mean the instrument's temporal resolution is improved?

Is taking a crisp still picture of a fast moving object the same thing as catching
the actual movement of that object?

Michael O'Keefe wrote:

} I guess not, too.
} At both mags you can tell that the object moved and by how much (if you had a larger
} field of view for the first case, it would tell you how much).
} My guess would be that a movement would be "temporally resolved" if you could capture
} two sucessive frames showing the minimum possible change. For movement, I guess that
} that would be a translation of one atomic distance or bond-length.
}
} Xudong Fan wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Michael O'Keefe wrote:
} }
} } } } By the way, what is the official unit for "temporal resolution?" Hz? or sec?
} } } Since Hz is frequency, temporal resolution must be sec (or msec)...
} }
} } Okay, this helps me understand better. This means that the temporal resolution is
} } not just
} } for vibrating object. Resolution of 1sec means you can not resolve vibration higher
} } than 1Hz, No problem.
} } What does this 1sec mean if you wish to observe an object which is moving in one
} } direction?
} } My definition: you can only capture the motion that is taking more than 1sec to
} } across the
} } screen. For example, your scan rate must be longer than 1sec/frame, you only can
} } capture one
} } position of the object, the following frames catches nothing. So the motion is not
} } resolved.
} } However, if I keep this scan rate, and simply reduce the magnification, say by 10X,
} } now
} } I can capture the motion by capture 10 positions of the object. Now the same motion
} } is resolved.
} } Was the temporal resolution improved? I guess not.



From daemon Fri Feb 22 10:02:17 2002



From: zaluzec-at-microscopy.com
Date: Fri, 22 Feb 2002 09:52:21 -0600
Subject: List of SEM Service Lab to image integrated circuits

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Try this URL for a non-compreshensive list of commerical sites.

http://www.amc.anl.gov/Docs/NonANL/ComSites.html

Nestor

Your Friendly Neighborhood SysOp

===========================================

}
}
} I am looking for SEM services in the Western USA that have high
} resolution (FE) SEM capabilities (and experience) of integrated
} circuit samples. Planar, oblique, and cross sections of IC
} components would be necessary.
}
} We generally have a relatively low volume of samples. Does anyone
} know of a resource directory of services such as this? Any
} suggestion would be appreciated.
}
} Thank you
}
} Curtis Olson
}


From daemon Fri Feb 22 10:18:13 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 22 Feb 2002 11:13:39 -0500
Subject: Re: Microtomist's Formulary and Guide - Reprint

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Chipping in my 2 cents worth, "The Microtomist's Formulary and Guide" is the
proverbial gold mine of information.

"Monson, Frederick C." wrote:

} Sorry for those repetitions folks, but I think that this reprint is an
} opportunity for any who are new to older, histotechnique compendia. I don't
} know an Krieger, but I have purchased the book, and I use it often.
}
} Highly recommended for personal or library.
}
} Regards,
}
} Fred Monson
}
} } ----------
} } From: Krieger
} } Sent: Thursday, February 21, 2002 5:18 PM
} } To: Monson, Frederick C.
} } Subject: Re: Gray Available?
} }
} } BOOK #: 202473 ISBN #: 0-88275-247-2
} }
} } AUTHOR: GRAY
} }
} } TITLE: MICROTOMIST'S FORMULARY AND GUIDE
} }
} } PRICE: 84.50 REFRL/ARRGM TYPE: CLOTH
} }
} } Shipping $5.00 UPS
} }
} } AT PRSENT WE ARE UPDATING OUR LISTS AND PORTIONS OF LOOKUP ARE OFFLINE.
} }
} } THE MICROTOMIST'S FORMULARY AND GUIDE
} } Peter Gray,
} } 0-88275-247-2
} }
} } Pages: 808, Binding: Cloth,
} }
} } Description:
} } This is a known and recognized source reference work. The book includes a
} } treatise on the art of making microscopic slides from biological
} } specimens, as well as a classified list of the formulas and techniques used
} in this
} } art.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Feb 22 10:44:30 2002



From: :      smusante-at-asmusa.org (by way of Caroline Schooley)
Date: Fri, 22 Feb 2002 08:31:29 -0800
Subject: Call for Materials - Deadline 3/1

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This has just appeared on the American Society for Microbiology's
listserver; I'm forwarding it because it may interest many on this list
also.

Do you have:
Stunning photographs of microorganisms?
Time-lapse video photography of microbial processes?
Innovative classroom activities?
A laboratory that engages students in their own learning?

If so, consider submitting materials you have authored to the ASM's
MicrobeLibrary - a now fully searchable database of more than 500 resources
for microbiology education.

All submissions received before March 1 will be reviewed in the upcoming
cycle. Visit {http://www.MicrobeLibrary.org} and go to the Submissions
page for details or contact {MicrobeLibrary-at-asmusa.org} with any questions.

Sincerely,
Susan

Susan Musante
Manager, Education Programs
Education Department
American Society for Microbiology
1752 N Street, N.W.
Washington, D.C. 20036-2904
phone: 202-942-9282
fax: 202-942-9329
smusante-at-asmusa.org
http://www.asmusa.org
http://www.microbelibrary.org

Register now for the ASM's Undergraduate Microbiology Education Conference
http://www.asmusa.org/edu4c2002.htm





Unsubscribe or access your membership settings at:
http://mail.asmusa.org/scripts/lyris.pl?enter=pcedu




From daemon Fri Feb 22 10:49:51 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 22 Feb 2002 08:47:54 -0800
Subject: RE: Nikon Coolscan 8000ED - option

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Please do see what you can find out.
Check out the Minolta too. It is advertised as being
able to directly handle TEM negs. It has some
sort of adjustable mask affair.

The unit has good specs and good reviews.
Price is not bad. But does it work?

gary

At 05:53 AM 2/22/2002, you wrote:
} Gary,
} I had not heard that about the 8000. They have been shipping
} slowly but
} steadily. We receive one every 3-5 weeks.
} I am attending the PMA show starting this Sunday and I am meeting
} with
} Nikon the same day. I'll see what I can find out.
}
} You are probably aware but keep in mind that the 8000 will not
} scan an
} entire 3.25 x 4 TEM neg at one time. Max scan width is only 56.9mm to
} 63.5mm depending on the film holder used.
}
} Thanks!!
}
} George
} George Laing
} National Graphic Supply
} v:(800) 223-7130 x3109
} f:(800) 832-2205
} email: scisales-at-ngscorp.com
} -----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: Thursday, February 21, 2002 7:00 PM
} To: scisales-at-ngscorp.com
} Cc: MSA listserver
} Subject: Nikon Coolscan 8000ED - option
}
}
} I've been hearing that the Coolscan 8000 was
} pulled from distribution. Mostly (??) due to
} bad LED and CCD mating.
}
} From recent research, if you want to scan from 35mm
} on up to TEM negs, look at the Minolta Dimage Scan Multi Pro.
} it is a 4.8D, 4800dpi 35mm, 3200dpi MF scanner with
} fluorescent lamp light source (very good). It interfaces
} via Ultra SCSI or Firewire. Supposedly, only Win2K and
} WinME work with it on the PC.
}
} TriState Camera (NYC) lists this unit at $2749 vs. the
} MSRP of $2999. I have not tried it but am getting itchy.
}
} gary g.
}
}
} At 11:49 AM 10/25/2001, you wrote:
}
} } Rick,
} } Many of our customers are very happy with the 8000ED.
} } I have looked into putting TEM negs into it and it could be
} } done by modifying one of the 120/220 film holders.
} } The holders have a raised lip to keep the film in the
} } channel. I believe these could be removed and the film could then
} } just extend out past the scan opening. I have not been able to try
} } this as demand for the scanner has been very high.
} } Another excellent scanner for TEM negs is the Agfa
} } T2500 Duoscan. While lower in resolution(2500dpi optical) it has
} } a glassless carrier design that will enable scanning of an entire
} } TEM negative. It also will scan reflective originals.
} }
} } George



From daemon Fri Feb 22 13:42:19 2002



From: Richard Harris :      rjharris-at-uwo.ca
Date: Fri, 22 Feb 2002 14:40:07 -0500
Subject: Balzers 301 Freeze Fracture available

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We have a Balzer's 301 Freeze Fracture system available which was upgraded
with cryopump and full electronic/digital controls approx 10 years ago.
While currently not running (the cryopump needs a rebuild) the system is in
otherwise reasonable shape.
We wish to dispose of it due to retirement of the major system user and a
shift in research direction within the department.
Interested parties are asked to contact the sender below for further
information.

Richard Harris

Laboratory Supervisor
Research Imaging Resources
Department of Zoology
University of Western Ontario
London ON
CANADA N6A 5B7
rjharris-at-uwo.ca
(519) 661-2111 ext 86780
(519) 661-2014 Fax




From daemon Fri Feb 22 13:56:17 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 22 Feb 2002 11:51:42 -0800
Subject: Re: Questions (about resolution) on the SEM

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Dear Mr. Faerber,
I was recently evaluating VP-SEMs and one of the samples I used, which
proved to be quite difficult to image well, was dried but uncoated flower
material from the sensitive plant. This is a fine pollen, about five microns
in diameter, with tiny features on it. One SEM manufacturer never did image
the fine features. It was imaged either by low voltage or variable pressure
and made a good test. The low atomic number meant poor secondary and
backscattered electron yield.
The main reason gold on carbon is used is that more SE-1s are imaged, which
are quite indicative of the beam diameter, and there is less low-resolution
electrons from the beam interaction volume.
At 01:47 PM 2/22/02 +0100, you wrote:
}
} Why do we all test a new SEM befor buying with our own samples ? It's
} exactly why the gold of carbon sample is so far from our real needs, as
} Bebby said. It's good at high voltage, but its only real advantage is that
} all manufacturers use the same test, however the results may vary with the
} age of the sample etc...
}
} A nice test I do often with students, is to look at an egg shell : outher
} wall, inner wall, inner membrane, etc, coated or uncoated, at different
} energies, on a W-SEM and on a FE-SEM etc. It shows that in fact this
} "resolution" question in the SEM is a false problem. Is the word
} resolution attapted to what we speek from ? The real question is :
}
} "What do I see, and what am I missing ? "
}
} When peolpe speak from High Resolution SEM, they think always of FE-SEM.
} With a FE source, all is not done. To perform HR, I must have of course a
} FE gun, but also a design of the optic optimised for primary energy for a
} few hundred eV to a few keV (high voltage is always good), and a choice of
} detectors which allows to separate or mix SE and BSE.
}
} So, the set of parameters I will choose depends of the sample and of which
} probleme I have to solve. The type of source is one element among a lot :
} FE or W fil i.e. more or less brightnes, current, chromatic
} aberration, spot size
} WD, i.e. more or less depth of field. And what does depth of field
} mean, whith a 100k X magnification ? WD gives also more or less SE/BSE in
} the classical ET detector.
} Primary energy, i.e. more or less interaction volume, (or other
} said variation in the ratio between surface and volume informations), more
} less SE/BSE yield.
} Detector, i.e. the choice between BSE, SE, or a mixing of the two
} signals, in conjonction with the primary energy.
}
} It's a new thing for a lot of SEM user, to have to precise which detector
} has been used to perform the image they are showing. Most people are (more
} or less...) familiar with the WD and kV indication, but realy less with
} precision about "in lens-SE ", classical (Everhart-Thornley) or BSE
} detectors. I have made a test with a interresting sample (carbon nanotubes
} grown on a Co film on a Si (100) single crystal. The same location, at the
} same magnification at two energies, with the different detectors avaible,
} and I obtained 8 differents pictures. A closer look leaves 6 really
} different pictures, with different resolutions, but with different
} informations too. By the way, it would be nice if the manufacturer could
} "standardise" the name of their "In Lens", "Throught the lens", "Upper",
} "SE" detetcors !
}
} And other aspect must be mentionned, which is the ability to perform
} "good" resolution images of insulators, without coating. The performences
} at low energy of the HR-SEM is in that case the key element, and the
} resolution is not the main factor. We have performed images of organic
} compounds deposed on an teflon (!) film on glass (!). In a classical SEM,
} or older FE-SEM, we could not observe anything, because charging. With
} gold coating, at high energy the surface information is lost, and at low
} energy, the resolution is too poor. In recent HR-SEM from all brand, we
} could do it, with correct resolution (crystals from 100 to 300 nm at x
} 100k ), but at 0.5 to 1.5 keV, SE detector, and less enought charging to
} perform an image (of course without any coating).
}
} It would be interresting to propose a set of "testing samples" of
} different atomic number combinaisons, light and light, heavy and heavy,
} light on heavy and vice versa, dry or hydrated, inorganic or organic,
} which could everyone may himself or obtain easy, to perform
} resolution/information tests at different energy, WD, detector, etc.
} Perheps such a inventory soon exists ?
}
} One could take, for exemple :
} gold on carbon (to continue the tradition)
} carbone nanotubes dispersed on a heavy metal (gold is expensive,
} but why not a W foil)
} CVD silcon can grow with nice crystallite, wiskers, in particular
} when there are impurities
} insulators such as Al2O3, glasses, ceramics (Chris Jeffree told me
} from the inner surface from a broken halogen lamp, i.e. W crystallites
} "moving" on the glass. Is it right?)
} catalysers (heavy metals in ceramic powder, arc owen C nanotubes
} and its catalysr) to test spatial resolution of the BSE detectors in compo
} mode.
} etc...
}
} I am not biologist, so I have no idee what could be a good biological test
} specimen, in dry or cryo mode.
}
} Other idees ? Yes, certainly !
}
}
} J. Faerber
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Fri Feb 22 15:23:38 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Fri, 22 Feb 2002 15:00:41 -0600
Subject: Poor quality diamomd knife

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Dear listies,

Has anyone had experiences with diamond knifes that appear to have a poor
quality edge that degrades (breaks) with common safe use?

I have a particular knife (3mm ) that has "dinks" in the edge creating knife
marks. I won't comment on the brand unless contacted personally.

I am using the knife on skin and feather barbs, but I've used an older knife
as a comparison and it doesn't have the same problem as the newer knife.

Help!!!!!!
Tim Quinn
University of Kansas
Research Assistant
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu


From daemon Sat Feb 23 11:17:05 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Fri, 22 Feb 2002 12:56:57 -0500
Subject: Active or Passive Frame Grabber for aqnalogue SEM?

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I have a Philips 515 SEM (with analogue image formation) and I wish to print digital images from it.
What are the advantages/disadvantages of using an active vs. a passive slow scan image grabber to
acquire images?




From daemon Sat Feb 23 15:48:14 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Sat, 23 Feb 2002 16:35:41 -0500
Subject: Microtomist's Formulary and Guide - Addresses

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Apologies to all for not noticing that the info below didn't include
address, etc.

URL: http://www.krieger-publishing.com/


Krieger Publishing P.O. Box 9542 Melbourne, Florida U.S.A. 32902-9542 Phone (321) 724-9542 · Fax (321) 951-3671

Suffering from old-age and hockey fever at the same time is HELL!!! Go
North America!!!!

Fred Monson

} ----------
} From: Krieger
} Sent: Thursday, February 21, 2002 5:18 PM
} To: Monson, Frederick C.
} Subject: Re: Gray Available?
}
} BOOK #: 202473 ISBN #: 0-88275-247-2
}
} AUTHOR: GRAY
}
} TITLE: MICROTOMIST'S FORMULARY AND GUIDE
}
} PRICE: 84.50 REFRL/ARRGM TYPE: CLOTH
}
} Shipping $5.00 UPS
}
} AT PRSENT WE ARE UPDATING OUR LISTS AND PORTIONS OF LOOKUP ARE OFFLINE.
}
} THE MICROTOMIST'S FORMULARY AND GUIDE
} Peter Gray,
} 0-88275-247-2
}
} Pages: 808, Binding: Cloth,
}
} Description:
} This is a known and recognized source reference work. The book includes a
} treatise on the art of making microscopic slides from biological
} specimens,
} as well as a classified list of the formulas and techniques used in this
} art.
}
} -----Original Message-----
}
} Date: Friday, February 15, 2002 8:05 AM
} Subject: Gray Available?
}
}
} } Do you still have Gray, Microtomist's formulary and Guide? And, is it on
} a
} } web page? Can't recommend it if it isn't there!
} }
} } Regards,
} }
} } FCM
} }
} } Frederick C. Monson, PhD
}
}
}


From daemon Sat Feb 23 19:59:12 2002



From: nicholas.welham-at-murdoch.edu.au ()
Date: Sat, 23 Feb 2002 19:47:31 -0600
Subject: Ask-A-Microscopist: Leitz Ortholux Objective

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (nicholas.welham-at-murdoch.edu.au) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
February 23, 2002 at 19:28:55
---------------------------------------------------------------------------

Email: nicholas.welham-at-murdoch.edu.au
Name: Nick Welham

Organization: Murdoch University

Education: Graduate College

Location: Perth, WA, AUSTRALIA

Question: I have an old finite tube Leitz Ortholux I and have been
given about half a dozen infinity objectives which I'd like to use.
Does anyone know of a company which makes the appropriate adaptor?
regards
Nick

---------------------------------------------------------------------------


From daemon Sat Feb 23 21:30:57 2002



From: PESTOEM-at-aol.com
Date: Sat, 23 Feb 2002 22:23:18 EST
Subject: TEM Service on East Coast

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I am responding to Kathy Smith's question of independant service providers
on the East Coast.
We are providing TEM service for the last 35 years. We service Philips,
Zeiss, Jeol
& Hitachi instruments. We are located close to Philadelphia, PA and cover the
entire
East Coast.
Peter A. Stolzenberg, PESTO Inc., 215-699-6160 FAX 215-699-5275


From daemon Sat Feb 23 22:47:00 2002



From: Gary M. Easton :      gary.easton-at-scannerscorp.com
Date: Sun, 24 Feb 2002 10:59:07 -0500
Subject: Fw: Active or Passive Frame Grabber for aqnalogue SEM?

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Greg,

A quick answer is- advantages of an active system can be divided in two
categories.

1) Principal. An active system will create new capabilities, which your SEM
doesn't have now. For example, modern PC or a Mac. computer control of
applications like electron beam lithography and EDX imaging, which depend on
synchronization of either beam blanker or an EDX spectrometer with the
e-beam position. Another principal advantage, is that a large portion of SEM
electronics (most of the scan generation and video signal processing related
circuits) will no longer be needed. This will make an SEM more reliable and
less expensive to maintain.

2) Non-principal. An active system will allow much slower scan speed than
the SEM scan generator, and much longer integration times (pixel dwell
times) for video signal acquisition. That will allow you to acquire higher
resolution images easier than with the passive system. Also, active system
can better compensate for AC fields related interference, as compared with
the passive system.

Active system is more expensive than the passive one. A motorcycle is more
expensive than the bicycle. I wouldn't call it a disadvantage.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Greg Barclay {gbarclay-at-trinidad.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, February 22, 2002 12:56 PM


Greg,
Having sold both type of systems for many years now, I'll give you my
$.02 worth on the merits of a passive system.

1. While many active systems boast resolutions in excess of 4k x 4k, the
time to acquire images at these high resolutions make you think twice. Most
SEM manufacturers went to great pains to develop highly accurate, very low
drift scan generators to drive their photo crt. Why not take advantage of
it? A passively acquired 2k x 2k image takes the same amount of time as the
SEM photo scan - typically about one minute. And, on older SEM's like
yours, you can select different line dwell speeds to increase collection
efficiency.

2. What you see is what you get - that includes on screen data, micron
line markers, text, etc. Since the data(which is computed by the SEM) is
acquired along with the image, there is no question as to its accuracy of
calibration. As long as the SEM is properly calibrated, your passive system
is also - automatically. With an active system, its scans have to be
calibrated to mimic the ones produced by the SEM. If they are not, then
your active system will give you inaccurate results.

3. Passive systems will accept EDS dot mapping signals which you can
colorize and save.

4. An active system will require that the SEM to be fitted with some type
of external scan interface to enable the computer to drive the scan coils.
If it does, fine. If not, one will have to be installed before the system
can be used. The passive system simply syncs up to the existing SEM scans.
Installation is usually very simple and easy.

Active systems are also very good and will give you added capabilities(beam
steering) that a passive system won't, especially when integrated with an
EDS system. However, don't accept the advice that a passive system is of
any lesser quality or usefulness - it's all in what you intend to use it for
now, and in the future.


Gary M. Easton, President
Scanners Corporation
SEM Service/PC Based Imaging & EDS Sales
90 Aileron Court
Suite 6
Westminster, MD 21157
410.857.7633 x102(Voice)
410.857.7636(Fax)

} Greg,
}
} A quick answer is- advantages of an active system can be divided in two
} categories.
}
} 1) Principal. An active system will create new capabilities, which your
SEM
} doesn't have now. For example, modern PC or a Mac. computer control of
} applications like electron beam lithography and EDX imaging, which depend
on
} synchronization of either beam blanker or an EDX spectrometer with the
} e-beam position. Another principal advantage, is that a large portion of
SEM
} electronics (most of the scan generation and video signal processing
related
} circuits) will no longer be needed. This will make an SEM more reliable
and
} less expensive to maintain.
}
} 2) Non-principal. An active system will allow much slower scan speed than
} the SEM scan generator, and much longer integration times (pixel dwell
} times) for video signal acquisition. That will allow you to acquire higher
} resolution images easier than with the passive system. Also, active system
} can better compensate for AC fields related interference, as compared with
} the passive system.
}
} Active system is more expensive than the passive one. A motorcycle is more
} expensive than the bicycle. I wouldn't call it a disadvantage.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
} ----- Original Message -----
} } From: Greg Barclay {gbarclay-at-trinidad.net}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, February 22, 2002 12:56 PM
} Subject: Active or Passive Frame Grabber for aqnalogue SEM?
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I have a Philips 515 SEM (with analogue image formation) and I wish to
} print digital images from it.
} } What are the advantages/disadvantages of using an active vs. a passive
} slow scan image grabber to
} } acquire images?
} }
} }
} }
}
}
}
}




From daemon Sun Feb 24 12:48:32 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 24 Feb 2002 15:35:15 -0800
Subject: Re: FW: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



-----Original Message-----
} From: Ascend_jcr [mailto:ascend_jcr-at-att.net]
Sent: Sunday, February 24, 2002 10:28 AM
To: gbarclay-at-trinidad.net; Microscopy-at-sparc5.microscopy.com


Greg,

A quick answer is- advantages of an active system can be divided in two
categories.

1) Principal. An active system will create new capabilities, which your SEM
doesn't have now. For example, modern PC or a Mac. computer control of
applications like electron beam lithography and EDX imaging, which depend on
synchronization of either beam blanker or an EDX spectrometer with the
e-beam position. Another principal advantage, is that a large portion of SEM
electronics (most of the scan generation and video signal processing related
circuits) will no longer be needed. This will make an SEM more reliable and
less expensive to maintain.

2) Non-principal. An active system will allow much slower scan speed than
the SEM scan generator, and much longer integration times (pixel dwell
times) for video signal acquisition. That will allow you to acquire higher
resolution images easier than with the passive system. Also, active system
can better compensate for AC fields related interference, as compared with
the passive system.

Active system is more expensive than the passive one. A motorcycle is more
expensive than the bicycle. I wouldn't call it a disadvantage.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Greg Barclay {gbarclay-at-trinidad.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, February 22, 2002 12:56 PM


I think that an active system does create new capabilities.
It allows different aspect ratios and also variable pixel
dimensions. Additionally, unless the SEM allows for
legends to be turned off, only any active system will
capture a pure image (no alpha stuff) so that it can be
image processed later on. Many times, the alpha
creates anomalies during processing. Sometimes it
does not. Without alpha, it is not an issue.

I find that the requisite pixel dwell time is inversely
proportional to the pixel dimensions. i.e., the more
pixels, the shorter the dwell time. With small pixel
dimensions, longer pixel dwell time reduces noise
which also happens with larger pixel dimensions but
at shorter dwell times. Either reduce noise at small
pixel dimensions via longer dwell time or increase
pixel dimensions and reduce dwell time. For image
processing, I like 3088x2060 at 5uS. This replaces
the recording CRT's 1.33 aspect ratio to 1.5--which
fits nicely on a 35mm slide. Or, change it to 1.33
and output to 6x4.5cm slides. With an active system,
there is an option, with passive, not.

The SEM's scan system is of course necessary to
do stig, positioning, etc. Whether the EDS system
comes with beam control or not (of course it will),
unless the SEM is designed to allow external beam
control, one needs schematics and some soldering
to gain access to the scan driver amplifiers. But he
does not mention EDS requirements, just digital capture.
So all of the EDS stuff is irrelevant. If the SEM is
designed properly, its imaging system will be sync'd
to line frequency. Active systems can do this too.
Furthermore, some can add micron markers, other
data and text. It is optional for the operator to do
so or not.

A passive system is certainly going to be cheaper.
If that is an issue, go for a passive system. My system
is both active and passive. I don't use the passive mode.
Fortunately, my SEM provides slow scan info as RS-170.
This is easily framegrabbed to accompany a high resolution
active scan. Neat.

BTW, the current Rontec EDS system is active scan.
Does a nice job.

gary g.



At 10:32 AM 2/24/2002, you wrote:

} -----Original Message-----
} } From: Ascend_jcr [mailto:ascend_jcr-at-att.net]
} Sent: Sunday, February 24, 2002 10:28 AM
} To: gbarclay-at-trinidad.net; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Active or Passive Frame Grabber for aqnalogue SEM?
}
}
} Greg,
}
} I couldn't help comment on this situation as Mr. Feingold obviously has some
} hidden agenda or doesn't grasp the concepts fully. First, an active system
} will not create new capabilities. It simply duplicates what your SEM does
} with the addition of independant digital sampling. Yes, it can drive the
} beam, but rarely better than the original SEM hardware which you will still
} need, incidentally for correcting stig, alignments, etc. What he says about
} EDS is partially true but most EDS systems (including older ones) come with
} beam control packages. The advantage of getting the beam control from the
} EDS system is that they provide integrated software to do digital mapping,
} linescans, in addition to electron imaging. Just purchasing an archiving
} system, limits you to electron imaging: you would then have to undertake a
} multi-man-year project to integrate with the EDS spectrometer.
} None of the second part of Feingold's response is accurate. Slower dwell
} times generally do not improve the image for two reasons: First, is that you
} tend to build up charging and/or contamination on a stationary spot (are you
} familiar with the dark square left behind by the raster?). Secondly, all
} SEMs have a stage drift factor from signififcant to severe. Sitting on a
} stationary spot will actually result in a record of the stage drift. This is
} why the EDS companies provide elaborate drift compensation programs with
} their digital beam control packages.
}
} A further disadvantage of an active system is that it loses all instrument
} information unless it maintains a separate communication line with the SEM
} for recording kV, mag, etc.
}
} Passive systems are far superior for pure image collection applicatons such
} as digital image archiving. They retain the microscope information printed
} on the data bar. They can achieve higher resolution and in some cases
} perform frame averaging which is a for superior method of reducing noise
} over point averaging since charge doesn't build up.
}
} I have access to both types systems in addition to a system provided by Edax
} and would be happy to provide more detailed information if you want. Is
} Feingold trying to sell an active system? I didn't know there were any left
} on the market.
}
} Regards,
} Joe Robinson



From daemon Sun Feb 24 19:51:53 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 25 Feb 2002 14:42:07 GMT+1200
Subject: Re: FW: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I think the tone of the comments about Vitaly Feingold's
alleged agenda are a bit snide.

At least Vitaly is sufficiently upfront to include his affiliation
in his signature, as should everyone who posts to a list.

IMHO

cheers

rtch





} From: "Ascend_jcr" {ascend_jcr-at-att.net}
} To: "Net Gbarclay-at-Trinidad." {gbarclay-at-trinidad.net}
} Cc: "microscopy. com Microscopy-at-sparc5." {Microscopy-at-sparc5.microscopy.com}
} Subject: FW: Active or Passive Frame Grabber for aqnalogue SEM?
} Date: Sun, 24 Feb 2002 10:32:33 -0800
} Importance: Normal

} --------------------------------------------------------------------
} ---- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
} ---.
}
}
}
} -----Original Message-----
} } From: Ascend_jcr [mailto:ascend_jcr-at-att.net]
} Sent: Sunday, February 24, 2002 10:28 AM
} To: gbarclay-at-trinidad.net; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Active or Passive Frame Grabber for aqnalogue SEM?
}
}
} Greg,
}
} I couldn't help comment on this situation as Mr. Feingold obviously
} has some hidden agenda or doesn't grasp the concepts fully. First,
} an active system will not create new capabilities. It simply
} duplicates what your SEM does with the addition of independant
} digital sampling. Yes, it can drive the beam, but rarely better than
} the original SEM hardware which you will still need, incidentally
} for correcting stig, alignments, etc. What he says about EDS is
} partially true but most EDS systems (including older ones) come with
} beam control packages. The advantage of getting the beam control
} from the EDS system is that they provide integrated software to do
} digital mapping, linescans, in addition to electron imaging. Just
} purchasing an archiving system, limits you to electron imaging: you
} would then have to undertake a multi-man-year project to integrate
} with the EDS spectrometer. None of the second part of Feingold's
} response is accurate. Slower dwell times generally do not improve
} the image for two reasons: First, is that you tend to build up
} charging and/or contamination on a stationary spot (are you familiar
} with the dark square left behind by the raster?). Secondly, all SEMs
} have a stage drift factor from signififcant to severe. Sitting on a
} stationary spot will actually result in a record of the stage drift.
} This is why the EDS companies provide elaborate drift compensation
} programs with their digital beam control packages.
}
} A further disadvantage of an active system is that it loses all
} instrument information unless it maintains a separate communication
} line with the SEM for recording kV, mag, etc.
}
} Passive systems are far superior for pure image collection
} applicatons such as digital image archiving. They retain the
} microscope information printed on the data bar. They can achieve
} higher resolution and in some cases perform frame averaging which is
} a for superior method of reducing noise over point averaging since
} charge doesn't build up.
}
} I have access to both types systems in addition to a system provided
} by Edax and would be happy to provide more detailed information if
} you want. Is Feingold trying to sell an active system? I didn't know
} there were any left on the market.
}
} Regards,
} Joe Robinson
}
}
} -----Original Message-----
} } From: Vitaly Feingold [mailto:vitalylazar-at-worldnet.att.net]
} Sent: Saturday, February 23, 2002 3:59 PM
} To: gbarclay-at-trinidad.net; Microscopy-at-sparc5.microscopy.com
} Subject: Re: Active or Passive Frame Grabber for aqnalogue SEM?
}
}
} --------------------------------------------------------------------
} ---- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
} ---.
}
}
} Greg,
}
} A quick answer is- advantages of an active system can be divided in
} two categories.
}
} 1) Principal. An active system will create new capabilities, which
} your SEM doesn't have now. For example, modern PC or a Mac. computer
} control of applications like electron beam lithography and EDX
} imaging, which depend on synchronization of either beam blanker or
} an EDX spectrometer with the e-beam position. Another principal
} advantage, is that a large portion of SEM electronics (most of the
} scan generation and video signal processing related circuits) will
} no longer be needed. This will make an SEM more reliable and less
} expensive to maintain.
}
} 2) Non-principal. An active system will allow much slower scan speed
} than the SEM scan generator, and much longer integration times
} (pixel dwell times) for video signal acquisition. That will allow
} you to acquire higher resolution images easier than with the passive
} system. Also, active system can better compensate for AC fields
} related interference, as compared with the passive system.
}
} Active system is more expensive than the passive one. A motorcycle
} is more expensive than the bicycle. I wouldn't call it a
} disadvantage.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
} ----- Original Message -----
} } From: Greg Barclay {gbarclay-at-trinidad.net}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, February 22, 2002 12:56 PM
} Subject: Active or Passive Frame Grabber for aqnalogue SEM?
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I have a Philips 515 SEM (with analogue image formation) and I wish to
} print digital images from it.
} } What are the advantages/disadvantages of using an active vs. a passive
} slow scan image grabber to
} } acquire images?
} }
} }
} }
}
}
Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Sun Feb 24 20:27:30 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sun, 24 Feb 2002 19:24:37 -0700
Subject: FW: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg,

Let me put in my 2 cents worth. We had discussed our ADDA off-line before,
and perhaps that prompted you to ask your question on the list server.

A SEM works by moving an electron beam across the sample and measuring the
interaction between the beam and sample at each point. SEM manufacturers
have put in a lot of effort to overcome the problems with electromagnetic
lenses, focusing and the like. The result are the high resolution
instruments of today. However, until a few years ago, there was no way of
recording the images other than through film. The medium of choice very
quickly became the Polaroid Instant Film, which although still a chemical
process, allowed quick and relatively easy dissemination of the images.

Most SEMs have a special photo mode, where the image is scanned with 1000 or
2000 lines and a total duration of a few minutes.

As Joe said, the digital scan interfaces do not try to replace all the
electronics of the microscope that the manufacturers put so much effort in.
Each SEM on the market, however, uses a similar chain of electronics. First
there is a scan generator which produces a precise voltage ramp that in the
end moves the beam across the sample. After that there is a scan amplifier,
which amplifies the voltage sweep to the actual voltage needed for the
sweep. This is determined by the magnification that is required. There is
more electronics for other aspects of the microscope such as tilt and
astigmatism correction, etc.

With a passive system, you leave the entire chain intact and "only" digitize
the signal as it is produced by the SEM. If you take the signal at the
monitor, that includes the signals that are displayed as micron bars, etc.
Of course that makes it easier to acquire the signals, but because there is
no change of the microscope, there are no additional capabilities. EDS dot
maps can be read, but again you depend on another piece of equipment to give
you the signals and the correlation with the beam position. In most cases
users are interested in getting high quality images, which means that you
would run the SEM in photo mode. If your SEM is limited to 1000 lines, the
images you get are roughly 1200 x 1000 (determined through the aspect ratio
of the screen), or 2400 x 2000 if you have a 2000 line photo mode. Depending
on how easy it is to change the photo parameters, it could be simple or hard
to change the parameters for the images (for example dwell time, etc.)

With an active system, the digital interface includes a scan generator.
While the system is active, it replaces the SEM internal scan generator, but
uses all of the other electronics. Aside from the fact that one does not
have to replace all the electronics, this also preserves all the settings
for astigmatism and other parameters between switching to internal or
external. Since the scan generator is now under direct control of the
computer, the beam can be moved wherever the computer dictates, and kept at
any point as long as the computer decides. This allows the computer total
freedom of image resolution and aspect ratio (for example a very rapid frame
rate at a reduced resolution for focusing, or a high resolution at longer
dwell times for high quality images.) It is also easier to acquire dot maps
as the computer knows at any time where exactly the beam is and only needs
the number of counts from the EDS system. In terms of image resolution,
there is a trade-off: The beam position is determined by the voltage the
scan generator produces, modified by the scan amplifier. A normal scan
voltage is of the order of a few volts. If this is digitized into 1000
positions, the voltage for 2 neighboring positions differ by a few
millivolts. All the electronics adds noise, so if you go to high in
resolution, the voltage difference of two neighboring pixels can be of the
same order as the noise on the signal. In other words, going too high in
resolution could add significant time to the acquisition but not result in
better images.

Which one is better? I don't think there is a final answer to this. If your
goal is to simply digitize the images on the SEM, and you don't have any
issues with the resolution and aspect ratios, etc, a passive system will
work just fine. If you need higher resolution, need to control dwell time
better, or need other control of the SEM, a passive system will not give you
enough possibilities. Or make sure that the passive system is upgradeable to
an active system if that becomes necessary at some time.

'nuff said. I wasted too much bandwidth already...

mike



} } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Ave #300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a Philips 515 SEM (with analogue image formation) and I wish to
print digital images from it.
} What are the advantages/disadvantages of using an active vs. a passive
slow scan image grabber to
} acquire images?
}
}
}




From daemon Sun Feb 24 22:27:26 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 24 Feb 2002 20:09:55 -0800
Subject: RE: FW: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Where is your response relative to my posting?
Do you have a way of deliniating your input
from others'?

Otherwise, I cannot sort out your nonsense
from all other.

gg

At 07:49 PM 2/24/2002, you wrote:


} -----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: Sunday, February 24, 2002 3:35 PM
} To: Ascend_jcr
} Cc: MSA listserver
} Subject: Re: FW: Active or Passive Frame Grabber for aqnalogue SEM?
}
}
} I think that an active system does create new capabilities.
} It allows different aspect ratios and also variable pixel
} dimensions. Why would you want non-square pixels? Philips did this with
} their XL system and destroyed any possibility of ever doing image analysis
} with the images.
} Additionally, unless the SEM allows for
} legends to be turned off, (all indeed provide this capability) only any
} active system will
} capture a pure image (no alpha stuff) so that it can be
} image processed later on. Many times, the alpha
} creates anomalies during processing. Sometimes it
} does not. Without alpha, it is not an issue. It is not an issue on any SEM
}
} I find that the requisite pixel dwell time is inversely
} proportional to the pixel dimensions. i.e., the more
} pixels, the shorter the dwell time. With small pixel
} dimensions, longer pixel dwell time reduces noise
} which also happens with larger pixel dimensions but
} at shorter dwell times. This is baloney. The pixel size is related to the
} DACs digital to analog conversion.
} Either reduce noise at small
} pixel dimensions via longer dwell time or increase
} pixel dimensions and reduce dwell time. For image
} processing, I like 3088x2060 at 5uS. This replaces
} the recording CRT's 1.33 aspect ratio to 1.5--which
} fits nicely on a 35mm slide. Or, change it to 1.33
} and output to 6x4.5cm slides. With an active system,
} there is an option, with passive, not.
} Passive has a full choice of matrices.
}
} The SEM's scan system is of course necessary to
} do stig, positioning, etc. Whether the EDS system
} comes with beam control or not (of course it will),
} unless the SEM is designed to allow external beam
} control, one needs schematics and some soldering
} to gain access to the scan driver amplifiers. But he
} does not mention EDS requirements, just digital capture.
} So all of the EDS stuff is irrelevant. If the SEM is
} designed properly, its imaging system will be sync'd
} to line frequency. Active systems can do this too.
} Furthermore, some can add micron markers, other
} data and text. It is optional for the operator to do
} so or not.
}
} A passive system is certainly going to be cheaper.
} If that is an issue, go for a passive system. My system
} is both active and passive. I don't use the passive mode.
} Fortunately, my SEM provides slow scan info as RS-170.
} This is easily framegrabbed to accompany a high resolution
} active scan. Neat.
}
} BTW, the current Rontec EDS system is active scan.
} Does a nice job.
}
} gary g.
}
}
}
} At 10:32 AM 2/24/2002, you wrote:
}
} } -----Original Message-----
} } } From: Ascend_jcr [mailto:ascend_jcr-at-att.net]
} } Sent: Sunday, February 24, 2002 10:28 AM
} } To: gbarclay-at-trinidad.net; Microscopy-at-sparc5.microscopy.com
} } Subject: RE: Active or Passive Frame Grabber for aqnalogue SEM?
} }
} }
} } Greg,
} }
} } I couldn't help comment on this situation as Mr. Feingold obviously has
} some
} } hidden agenda or doesn't grasp the concepts fully. First, an active system
} } will not create new capabilities. It simply duplicates what your SEM does
} } with the addition of independant digital sampling. Yes, it can drive the
} } beam, but rarely better than the original SEM hardware which you will still
} } need, incidentally for correcting stig, alignments, etc. What he says about
} } EDS is partially true but most EDS systems (including older ones) come with
} } beam control packages. The advantage of getting the beam control from the
} } EDS system is that they provide integrated software to do digital mapping,
} } linescans, in addition to electron imaging. Just purchasing an archiving
} } system, limits you to electron imaging: you would then have to undertake a
} } multi-man-year project to integrate with the EDS spectrometer.
} } None of the second part of Feingold's response is accurate. Slower dwell
} } times generally do not improve the image for two reasons: First, is that
} you
} } tend to build up charging and/or contamination on a stationary spot (are
} you
} } familiar with the dark square left behind by the raster?). Secondly, all
} } SEMs have a stage drift factor from signififcant to severe. Sitting on a
} } stationary spot will actually result in a record of the stage drift. This
} is
} } why the EDS companies provide elaborate drift compensation programs with
} } their digital beam control packages.
} }
} } A further disadvantage of an active system is that it loses all instrument
} } information unless it maintains a separate communication line with the SEM
} } for recording kV, mag, etc.
} }
} } Passive systems are far superior for pure image collection applicatons such
} } as digital image archiving. They retain the microscope information printed
} } on the data bar. They can achieve higher resolution and in some cases
} } perform frame averaging which is a for superior method of reducing noise
} } over point averaging since charge doesn't build up.
} }
} } I have access to both types systems in addition to a system provided by
} Edax
} } and would be happy to provide more detailed information if you want. Is
} } Feingold trying to sell an active system? I didn't know there were any left
} } on the market.
} }
} } Regards,
} } Joe Robinson



From daemon Mon Feb 25 02:23:58 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Mon, 25 Feb 2002 19:18:59 +1100
Subject: Re: pollen viability (an answer)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just to add to this,
I was asked the same question a few weeks ago, and the combination of FDA,
as outlined by Kristen, and propidium iodide (PI), is also very good. PI
(at about 1-10 microgram/ml final concentration in water or buffer)
penetrates non-viable cells giving red fluorescence and provides a good
contrast to the green FDA, especially in pollen that is highly
autofluorescent. It can be excited with blue or green light - 488, 514 or
543 nm laser lines on confocal.
cheers, Rosemary

}
} Hi Soumitra,
} Yes, there is a quick and easy way. I don't have my original protocol, but
} this one will probably do (from Ruzin's Plant Microtechnique and
} Microscopy). Dissolve 1 mg of fluorescein diacetate (FDA) in 1 mL of
} acetone for a stock solution that can be stored at -20C. Then add to pollen
} grains for a grand total concentration of 1 microgram FDA/mL and incubate
} for 5 minutes. Observe under blue light (488 nm). Living cells will
} fluoresce yellow/green. My pollen was usually in germination medium at the
} time so that is into what I added the FDA. I'll cross-check this with my
} old files and let you know if there are any significant differences. Good
} luck.
} Kristen
}
}
}
} Kristen A. Lennon, Ph.D.
} Department of Plant Pathology
} 351 Bessey Hall
} Iowa State University
} Ames, IA 50011
} 515-294-8854
} kalen-at-iastate.edu
} www.baumlab.org


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au




From daemon Mon Feb 25 04:28:48 2002



From: =?iso-8859-1?Q?Dal=E9ne?= Josling :      djosling-at-op.up.ac.za
Date: Mon, 25 Feb 2002 12:19:57 +0200
Subject: Looking for Ron Anderson

Contents Retrieved from Microscopy Listserver Archives
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Good day

I'm urgently looking for Mr Ron Anderson.
The last e-mail I've got is :
anderron-at-US.ibm.com

If anyone can help me I would really appreciate it.
You can contact me on my e-mail.

Thanks in advance
Daléne Josling



From daemon Mon Feb 25 07:46:52 2002



From: JHoffpa464-at-aol.com
Date: Mon, 25 Feb 2002 08:38:38 EST
Subject: tem service on the east coast

Contents Retrieved from Microscopy Listserver Archives
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take it from someone that has been around em for over 20 years. it is well woth the extra money for the piece of mind that comes from having a service contract with the the compnay you bought your scope from. it may be less expensive to go with a private company like presto, however the costs down the road in parts, if they can be found and in down time, likly to be longer. like i always say stick with what you know. companies like philips and jeol are know quanites and will be around for a long time.
just my nickels worth. you know the price of inflation and my experience leads me to charge more...
john
all typos are the result od a sticky keyboard.


From daemon Mon Feb 25 08:26:04 2002



From: Erasmus, Willem (WJ) :      willem.erasmus-at-sasol.com
Date: Mon, 25 Feb 2002 16:18:30 +0200
Subject: Monte Carlo simulations

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Dear listmembers

I need to calculate the interaction volume of the SEM electron beam in my specimen. The Monte Carlo simulation packages that I encountered so far only calculates the volume for pure elements. But I sometimes have oxides with densities that are different from that of the pure metals (something like Al2O3). Is there a program that can calculate the interaction volumes for these materials ?

Thanks in advance
Willem Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]




From daemon Mon Feb 25 09:45:57 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 25 Feb 2002 09:38:05 -0600
Subject: Re: FW: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
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Another 2 cents worth on digital imaging on the SEM:

MAINS FREQ INTERFERENCE:

SEMs are almost always affected by mains frequency stray field. As a
result the scan generators always lock their horizontal signal to
the line frequency. This means that the stray field becomes a
distortion rather than a blur. When using a small, rapid scan for
focusing and astigmatism correction, this distortion will cause the
image to undulate, unless the vertical scan signal is also
line-locked.

I don't know of any stand alone active digital scan systems that do
this. If they exist, it would be important to plug them into a wall
socket using the same phase of the 3-phase power (normally present in
laboratories) as is used to run the SEM itself.

More to the point, an active system must use some sort of wire to
connect the scan/memory computer to the EM electronics. In spite of
all the disclaimers about how "This is not problem with modern
electronics and grounding techniques" (claims usually made by
digital, not analog, guys), I have never seen any such system that
does not create ground loops and hence exacerbate the
mains-frequency interference problem mentioned above.

You may not notice it at first if you aren't looking for it but it
will be there, especially at low voltage.

Verdict: A strong advantage for passive systems, especially those
that make serious efforts to avoid ground loops associated with the
video-signal wire (Linearized optical couplings?).

"SIGNAL INTEGRATION"

Another big variable in SEM memories often ignored is the matter of
sampling. Early frame grabbers often were converted from video
frame-stores. These integrated the signal presented to the ADC for
about 60-100 ns, the time needed for a video pixel. However, when
recording a 30 second scan with 1000 x 1000 pixels, the pixel dwell
time is 30 microseconds, 300x longer. If the integration constant on
the ADC wasn't changed (and assuming that of the SEM signal amp was
fast), the digitally recorded image was mush noisier than one
recorded analog from the screen because it represented only 1/300 th
of the signal. Manufacturer's soon changed the circuitry to make the
integration constant vary with the scan frequency and raster size
(i.e., with the pixel dwell time) but this is easier to do if the
digital memory is built into the SEM from the start than with an add
on.

Moral here is that having a good a method to keep the time-constant
of the ADC proportional to the pixel dwell time at the scan speeds of
importance to you is just as significant as any other aspect of SEM
digital image memory design.
--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
3D Microscopy of Living Cells Course, June 10 - 20, 2002, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2002


From daemon Mon Feb 25 10:31:40 2002



From: Xudong Fan :      fanx-at-msu.edu
Date: Mon, 25 Feb 2002 11:30:45 -0500
Subject: Re: Questions on the Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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[... deleted .. ]

} Now here is another question.
} For a vibrating object, reduce magnification obviously won't catch the motion.
} But by blanking the beam periodically(as suggested by Gary and
} NASA JPL lab), a crisp image of the vibrating object can be resolved. Or by adjusting
} the blanking frequency, it can be seen in a slow motion.
} Does that mean the instrument's temporal resolution is improved?

I came up with my own answer: NO
This is how I understand the "beam blanking method" works:
Suppose an object vibrating at 1Hz between A and B, and the scan rate is
0.1Hz(10s/frame),
then I can not see the object moving. there is only an expanded image from A to B.
Now if I block the beam periodically at 1Hz. In one cycle, suppose the beam is blocked
for
0.9s and unblocked for 0.1s, so that we only can "see" the object within that 0.1s.
Since it is
synchronized, the object will always come back to the same position(e.g. A1) within
that 0.1s
period. Therefore I will resolve a crispy image at A1. Now if I change the beam
blanking
frequency slightly to 1.01Hz. After 10 cycle(while one frame is scanned), the object
does
not come back to the exact position A1, but a close by position A2. Then a second
image
will be captured. After 100 cycle, I will have 10 positions of the movement, so it
appears
that I have resolved it in a slow motion.

Unless my understanding above is wrong, there is no temporal resolution improvement in
this process. What this does is to take the information in 100 cycle(expands to 100s) and
reconstruct into 1 cycle. We can use this reconstructed 1 cycle to represent all the
cycles
ONLY because we have the prior knowledge that each cycle is absolutely identical.
In another word, if there is an anomaly within one cycle of the vibration, we won't know.
I believe there are something parallel in respect of spatial resolution.

Then what has been improved? In my opinion: spatial resolution.
In the beginning, we actual can resolve the motion by just change the scan rate from
10s/frame
to 0.1s/frame. Then I can see 10 position within one true cycle. What not using this rate?

Because it is a "fast scan", we won't see each image clearly. We know we can observe
better
spatial resolution at "slow scan", the obove "beam blanking method" allows us to obtain
10 clear images. Although the spatial resolution is improved, the true instrument temporal

resolution is REDUCED.

Here is my definition: the temporal resolution IS scan rate, i.e. if the scan rate is
0.1s/frame,
the temporal resolution is 0.1s.

Therefore, non-scanning microscopy(LM, TEM) has infinite temporal resolving power and only

limited by their aquisition system(eye, video camera, etc).

Imagine observing a slow flying bee(fast vibrating wings and slow moving body):
By using "beam blanking" method in a scanning microscope to get a "slow motion", we see:
Slow flapping wings, fast moving body.
By using an ultrafast vedio camera in non-scanning miroscope and re-play in slow motion,
we see:
Slow flapping wings, almost still body.




From daemon Mon Feb 25 11:31:33 2002



From: John :      Products-at-TonerBuys.com
Date: Sun, 24 Feb 2002 13:12:46 +-0800
Subject: Buy§Manufacture§Direct§Imaging§Supplies§Save§60%-80%

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From daemon Mon Feb 25 11:45:24 2002



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Mon, 25 Feb 2002 11:39:34 -0600
Subject: HPF of leaf tissue

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Has anyone out there had any success with high pressure
freezing/freeze substitution of plant leaves for TEM examination? If
so, please contact me as I haven't.

Bob

--
Robert R. Wise, Ph.D.
Associate Professor of Plant Physiology
Department of Biology and Microbiology
University of Wisconsin Oshkosh

On sabbatical from Sept. 2001 to Aug. 2002 at UW Madison
Botany Department
B217 Birge Hall
430 Lincoln Drive
Madison, WI 53706
(608) 262-4288 (phone)
(608) 262-7509 (fax)
wise-at-uwosh.edu
http://www.wisc.edu/biotron/Sharkey/
http://www.uwosh.edu/departments/biology/wise/wise.html


From daemon Mon Feb 25 12:10:52 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Mon, 25 Feb 2002 12:00:55 -0600
Subject: RE: Monte Carlo simulations

Contents Retrieved from Microscopy Listserver Archives
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Although rather expensive, I believe a program called Electron Flight
Simulator should do what you need.

The web address is: http://www.small-world.net/efs.htm

Woody White
McDermott Technology, Inc.
------------------------------------------------------------

}
} Dear listmembers
}
} I need to calculate the interaction volume of the SEM
} electron beam in my specimen. The Monte Carlo simulation
} packages that I encountered so far only calculates the volume
} for pure elements. But I sometimes have oxides with densities
} that are different from that of the pure metals (something
} like Al2O3). Is there a program that can calculate the
} interaction volumes for these materials ?
}
} Thanks in advance
} Willem Erasmus
}
} Willem Erasmus
} Snr. Scientist, Basic Catalysis Research
} Sasol Technology
} Tel : +27 +16 960 - 4211/2772
} Fax : +27 +16 960 - 2826
} E-mail : willem.erasmus-at-sasol.com
} PO Box 1, Sasolburg, 1947, Republic of South Africa
}
} [All views expressed are my own and not necessarily that of
} my employer.]
}
}
}


From daemon Mon Feb 25 12:14:58 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Mon, 25 Feb 2002 13:08:10 -0500
Subject: Re: tem service on the east coast

Contents Retrieved from Microscopy Listserver Archives
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John,
So glad you keep such an open mind. The sticky keyboard is also the
fault of third party service, right? Thought so.

DISCLAIMER: I have a vested interest in providing high quality service
to users of SEMs. It's how I make an honorable living and take care of
my family while helping a lot of wonderful people do THEIR jobs better.

Ken Converse
owner
Quality Images
third party SEM service since 1981
Delta, PA

"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Feb 25 13:18:27 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 25 Feb 2002 11:14:56 -0800
Subject: Re: FW: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
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Soft-Imaging ADDA locks to mains. I suspect that
Rontec's imaging system does too. ADDA allows
for locking or not. If you don't believe this, I can
send you a screen capture of the setup window
which offers this user option.

ADDA has a PCI board in the PC but communicates
to a separate D/A and A/D box via fiber optic cables.
The separate box is electrically at the same potential
as the SEM--it knows nothing about the potential at
the PC or any difference in potential between the
SEM and PC. It is a non-issue.

Furthermore, well-designed active systems use
a single wire for ground between the SEM and
drive electronics. The analog drive signals and
scan generator on/off are conveyed via shielded
cables which are only grounded at the driver side--
not at the SEM end. Another move to eliminate
ground loops.

Could you expand on the "signal integration" section?
I'm not following you, sorry. TV video is 15KHz
per line or 67uS. This is not what is being sampled.
It is slow scan that is being sampled. How often
is based on the number of pixels per line. That can
vary.

gary g.


At 07:38 AM 2/25/2002, you wrote:

} Another 2 cents worth on digital imaging on the SEM:
}
} MAINS FREQ INTERFERENCE:
}
} SEMs are almost always affected by mains frequency stray field. As a
} result the scan generators always lock their horizontal signal to the
} line frequency. This means that the stray field becomes a distortion
} rather than a blur. When using a small, rapid scan for focusing
} and astigmatism correction, this distortion will cause the image to
} undulate, unless the vertical scan signal is also line-locked.
}
} I don't know of any stand alone active digital scan systems that do this.
} If they exist, it would be important to plug them into a wall socket using
} the same phase of the 3-phase power (normally present in laboratories) as
} is used to run the SEM itself.
}
} More to the point, an active system must use some sort of wire to connect
} the scan/memory computer to the EM electronics. In spite of all the
} disclaimers about how "This is not problem with modern electronics and
} grounding techniques" (claims usually made by digital, not analog, guys),
} I have never seen any such system that does not create ground loops and
} hence exacerbate the mains-frequency interference problem mentioned above.
}
} You may not notice it at first if you aren't looking for it but it will be
} there, especially at low voltage.
}
} Verdict: A strong advantage for passive systems, especially those that
} make serious efforts to avoid ground loops associated with the
} video-signal wire (Linearized optical couplings?).
}
} "SIGNAL INTEGRATION"
}
} Another big variable in SEM memories often ignored is the matter of
} sampling. Early frame grabbers often were converted from video
} frame-stores. These integrated the signal presented to the ADC for about
} 60-100 ns, the time needed for a video pixel. However, when recording a 30
} second scan with 1000 x 1000 pixels, the pixel dwell time is 30
} microseconds, 300x longer. If the integration constant on the ADC wasn't
} changed (and assuming that of the SEM signal amp was fast), the digitally
} recorded image was mush noisier than one recorded analog from the screen
} because it represented only 1/300 th of the signal. Manufacturer's soon
} changed the circuitry to make the integration constant vary with the scan
} frequency and raster size (i.e., with the pixel dwell time) but this is
} easier to do if the digital memory is built into the SEM from the start
} than with an add on.
}
} Moral here is that having a good a method to keep the time-constant of the
} ADC proportional to the pixel dwell time at the scan speeds of importance
} to you is just as significant as any other aspect of SEM digital image
} memory design.
} --
} **********************************************
} Prof. James B.
} Pawley, Ph. 608-263-3147
} Room 223, Zoology Research
} Building, FAX 608-265-5315
} 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
} 3D Microscopy of Living Cells Course, June 10 - 20, 2002, UBC, Vancouver
} Canada
} Info: http://www.3dcourse.ubc.ca/ Applications due by March 15,
} 2002



From daemon Mon Feb 25 13:19:25 2002



From: maria olivia casanueva :      mocasanu-at-midway.uchicago.edu
Date: Mon, 25 Feb 2002 13:14:00 -0600 (CST)
Subject: Gal4 antibody

Contents Retrieved from Microscopy Listserver Archives
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Hi:

I am looking for an antibody that recognizes the DNA binding domain of
Gal4 and that can detect antigens in tissue using indirect
immunofluorescence or imunohistochemistry,
I would appreciate information!
Thanks




From daemon Mon Feb 25 13:20:36 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 25 Feb 2002 11:19:06 -0800
Subject: Re: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
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I think that a major factor about SEM digitization
is being underestimated. If not ignored--regardless
of active or passive issue.

Software. The software that accompanies the hardware
is perhaps more important and has more impact on
results than does the hardware. Simply capturing
an image is one thing, but being able to work with
the image, filter it, modify it, archive it, index it, etc.
are big factors I would think in the grander scheme.

gary g.



From daemon Mon Feb 25 13:28:16 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 25 Feb 2002 11:23:47 -0800
Subject: Re: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
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Dear Vitaly,
While I don't really want to wade into the various advantages of active vs.
passive digital imaging, I would like to correct a misconception presented
by several people about EDS imaging. The Quartz XOne EDS system now offers
completely passive, full-spectrum mapping and line scans, based on the
passive imaging capabilities of the Quartz PCI system. The maps are
accumulated over several scans at the line resolution of your SEM and this
makes the acquisition of 1024 X 840 x-ray maps in ten to fifteen minutes
quite feasible.
Also, by integrating and averaging several high-resolution scans, many of
the advantages of the longer dwell times available in the active system can
be realized by the passive.
Disclaimer: I have been involved in the design, testing and selling of the
Quartz XOne EDS system since its inception.
At 06:59 PM 2/23/2002 -0500, you wrote:
}
} Greg,
}
} A quick answer is- advantages of an active system can be divided in two
} categories.
}
} 1) Principal. An active system will create new capabilities, which your SEM
} doesn't have now. For example, modern PC or a Mac. computer control of
} applications like electron beam lithography and EDX imaging, which depend on
} synchronization of either beam blanker or an EDX spectrometer with the
} e-beam position. Another principal advantage, is that a large portion of SEM
} electronics (most of the scan generation and video signal processing related
} circuits) will no longer be needed. This will make an SEM more reliable and
} less expensive to maintain.
}
} 2) Non-principal. An active system will allow much slower scan speed than
} the SEM scan generator, and much longer integration times (pixel dwell
} times) for video signal acquisition. That will allow you to acquire higher
} resolution images easier than with the passive system. Also, active system
} can better compensate for AC fields related interference, as compared with
} the passive system.
}
} Active system is more expensive than the passive one. A motorcycle is more
} expensive than the bicycle. I wouldn't call it a disadvantage.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Mon Feb 25 14:30:22 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 25 Feb 2002 14:20:57 -0600
Subject: Re: Monte Carlo simulations

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dear William,

Simple Monte Carlo Programs do not account for the distortion of the
trajectories caused by charge build-up in insulators.

I actual fact this may be the major factor in determining the size
and shape of the interaction volume (not to mention the shape of the
probe that actually reached the surface of an uncoated insulator)

Experiments in this field often go back to EDX studies of frozen
aqueous solutions in which the tail of the Bremsstralung consistently
failed to reach the beam voltage and the beam was later shown not to
have "penetrated" as far as it "should" for this Z and density.

As charging itself is extremely complex, I would state it as my
opinion that Monte Carlo simulations of beam penetration in
insulators are of marginal utility unless you are only interested in
the first few microseconds of beam residence time on a surface known
not to have any trapped charge to start with.

Cheers,

Jim P.

--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
3D Microscopy of Living Cells Course, June 10 - 20, 2002, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2002


From daemon Mon Feb 25 15:28:21 2002



From: dsaja-at-cmnh.org
Date: Mon, 25 Feb 2002 16:25:01 -0500
Subject: Joel SEM, riggers needed to move NJ to OH

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{color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Times New Roman {/param} {bigger} Greetings,


I needed a company to move a Joel SEM with EDX from
central New Jersey to Cleveland Ohio.


The Cleveland Museum of Natural History has had an offer to
have a Joel SEM donated. The catch is that it is in the basement
of a consultants house. Does anyone know of a company in the
Ohio-Pennsylvania-New Jersey (New York?) area who is, at
the least, capable of hoisting it out of his basement and onto a
truck? We may also be interested in delivery and re-assembly
and alignment.


Thank you for any suggestions,

Dr. David Saja, Geologist

dsaja-at-cmnh.org {/color} {FontFamily} {param} Courier New {/param} {smaller}

{nofill}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. David Saja (216) 231-4600 x429
Curator of Mineralogy FAX: (216) 231-5919
dsaja-at-cmnh.org

The Cleveland Museum of Natural History
1 Wade Oval Drive, University Circle
Cleveland, Ohio 44106-1767
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Mon Feb 25 15:33:08 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 25 Feb 2002 14:29:08 -0700
Subject: Re: FW: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
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Good points (and definitely worth more than 2 cents!).

Mains Frequency Interference: Yes, this is quite obvious sometimes. For that
reason our system does include the synch electronics. You pay a small price
in terms of speed. Since each line has to be synched to a certain phase of
the power signal, each line is delayed a bit, leaving to a slighly lower
frame rate. I can only speak for our electronics, but I am sure others have
that as well.

Ground loops: Yes, they can be a problem. That is why we try to keep the
copper wires as short as possible by placing a box somewhere near the SEM,
and then connect the box to the PC via an optical connection. Of course the
optical connection is immune to electro-magnetic interference, but you're
right, if you are not careful, even the shortest cables can suffer from
interference. And if you look in the back of your SEM, there are plenty of
cables that could interfere! Sometimes, at the highest magnifications,
mechanical vibrations may be confused with electrical interference.

Signal Integration: I would consider this an advantage of the active system,
as the computer can determine the dwell time as opposed to a passive system
where the SEM determines the dwell time and the computer has to "guess". But
you are of course correct, in that it is not sufficient to simply use a 60
ns integration time. However, keep in mind, that the X-direction in older
SEMs is NOT digitized. It is an analog signal. There is no real "dwell time"
as the beam is in constant movement. The "x-resolution" is then determined
by the number of lines, the requirement to have square pixels, the aspect
ratio of the screen, and of course the time for one line scan. For example,
if you use a 1000 line photo setting, the final picture should have
something like 1280 x 1000 pixels (The aspect ratio of the SEM screen is
usually 4:3). For a total frame time of 2 min (120 sec), the line time is
roughly 120 msec (120/1000), resulting in a "pixel itme" of roughly 100
microseconds (120 msec / 1280).

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
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fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
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-----Original Message-----
} From: James Pawley [mailto:jbpawley-at-facstaff.wisc.edu]
Sent: Monday, February 25, 2002 8:38 AM
To: Microscopy-at-sparc5.microscopy.com


Another 2 cents worth on digital imaging on the SEM:

MAINS FREQ INTERFERENCE:

SEMs are almost always affected by mains frequency stray field. As a
result the scan generators always lock their horizontal signal to
the line frequency. This means that the stray field becomes a
distortion rather than a blur. When using a small, rapid scan for
focusing and astigmatism correction, this distortion will cause the
image to undulate, unless the vertical scan signal is also
line-locked.

I don't know of any stand alone active digital scan systems that do
this. If they exist, it would be important to plug them into a wall
socket using the same phase of the 3-phase power (normally present in
laboratories) as is used to run the SEM itself.

More to the point, an active system must use some sort of wire to
connect the scan/memory computer to the EM electronics. In spite of
all the disclaimers about how "This is not problem with modern
electronics and grounding techniques" (claims usually made by
digital, not analog, guys), I have never seen any such system that
does not create ground loops and hence exacerbate the
mains-frequency interference problem mentioned above.

You may not notice it at first if you aren't looking for it but it
will be there, especially at low voltage.

Verdict: A strong advantage for passive systems, especially those
that make serious efforts to avoid ground loops associated with the
video-signal wire (Linearized optical couplings?).

"SIGNAL INTEGRATION"

Another big variable in SEM memories often ignored is the matter of
sampling. Early frame grabbers often were converted from video
frame-stores. These integrated the signal presented to the ADC for
about 60-100 ns, the time needed for a video pixel. However, when
recording a 30 second scan with 1000 x 1000 pixels, the pixel dwell
time is 30 microseconds, 300x longer. If the integration constant on
the ADC wasn't changed (and assuming that of the SEM signal amp was
fast), the digitally recorded image was mush noisier than one
recorded analog from the screen because it represented only 1/300 th
of the signal. Manufacturer's soon changed the circuitry to make the
integration constant vary with the scan frequency and raster size
(i.e., with the pixel dwell time) but this is easier to do if the
digital memory is built into the SEM from the start than with an add
on.

Moral here is that having a good a method to keep the time-constant
of the ADC proportional to the pixel dwell time at the scan speeds of
importance to you is just as significant as any other aspect of SEM
digital image memory design.
--
**********************************************
Prof. James B. Pawley, Ph.
608-263-3147
Room 223, Zoology Research Building, FAX
608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
3D Microscopy of Living Cells Course, June 10 - 20, 2002, UBC, Vancouver
Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15,
2002


From daemon Mon Feb 25 16:02:33 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 25 Feb 2002 15:54:57 -0600
Subject: Re: FW: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Soft-Imaging ADDA locks to mains. I suspect that
} Rontec's imaging system does too. ADDA allows
} for locking or not. If you don't believe this, I can
} send you a screen capture of the setup window
} which offers this user option.


I have no reason not to believe you. Many early systems lacked this
facility. I am glad that your does not.

} ADDA has a PCI board in the PC but communicates
} to a separate D/A and A/D box via fiber optic cables.
} The separate box is electrically at the same potential
} as the SEM--it knows nothing about the potential at
} the PC or any difference in potential between the
} SEM and PC. It is a non-issue.


I am glad that you use F/O couplings but, with respect, whether or
not it is "a non-issue" would be determined when I hooked your system
attached to my Hitachi S-900 FESEM working at 1kV, and found there
was no distortion at 200kx. I know of no more sensitive ground-loop
sensor.

I am not saying that it cannot be done, just that it seldom is done
and people are often not even aware that it can be a problem.

} Furthermore, well-designed active systems use
} a single wire for ground between the SEM and
} drive electronics. The analog drive signals and
} scan generator on/off are conveyed via shielded
} cables which are only grounded at the driver side--
} not at the SEM end. Another move to eliminate
} ground loops.

This all sounds fine but one usually adds a frame store to an old
scope (new ones have their own). AFTER they have been installed in
your lab such scopes often have grounding systems that have
surprising properties.


} Could you expand on the "signal integration" section?
} I'm not following you, sorry. TV video is 15KHz
} per line or 67uS. This is not what is being sampled.
} It is slow scan that is being sampled. How often
} is based on the number of pixels per line. That can
} vary.

I merely wish to point out that while photographic image collection
automatically integrates the signal for each pixel in the time domain
(even if it weren't already "time-averaged" by the slow response of
the CRT phosphor), this is not automatically the case for digital
frame stores.

Unless the system employs "box-car" integration (which is a great
idea! It is found on Bio-Rad confocal scopes) the bandwidth of the
preamp before the ADC determines an integration time. If this time is
less than the time that the SEM beam spends traversing one pixel in
the raster, the voltage sensed by the ADC will be averaged only over
part of the pixel time and the resulting data will be noisier than it
should be.

The problem is more important in the SEM than in other digitally
sampled imaging systems because the pixel-dwell time can vary widely:
from 0.1 microseconds at "tv-rate" to 1000x that for slow scan. The
signal preamp bandwidth on the scope must be wide enough to pass the
high-frequency "tv-rate" signal. But hat means that it is much too
wide to properly integrate the slow scan signal. The bandwidth of the
signal being fed to the ADC must be adjusted in the frame-store to
correspond to the pixel dwell time.

We won't go into how the PSF of the microscope itself limits the
bandwidth of the signal. (At low mag very large changes in signal
level from one pixel to the next are possible: at high mag, things
get fuzzy and large changes aren't possible.) But in principle, it
would be possible to use magnification and other information from the
SEM to impose additional limits on the bandwidth of the ADC. As I
remember, Everhardt proposed changing the bandwidth of the video amp
with the signal level and the scan speed in his Thesis in the 1950s.

Alternatively, John MacKensie recommends that when sampling "slow"
(long?) pixels, you use the same fast ADC-preamp settings but make
many measurements during the long pixel dwell time and then digitally
average the results before sending the value off to storage. Assuming
that this super-sampling rate was similar to the time constant of the
ADC preamp, this should work well.

This is not a small problem. Some years ago David Joy recorded white
noise images by having a defocused beam strike smooth specimen
without scanning. The only variations in the SE signal should have
been the statistical variations in the SE current caused by shot
noise. As he could measure this current, he could calculated what
shot-noise should be and then calculate a average-signal to
shot-noise ratio. He could also measure the standard deviation of the
image data stored in the memory and ratio this to the average
intensity.

If the detector had high quantum efficiency and the digitizer worked
properly, these two S/N ratios should be the same. (i.e., the data
recorded would have the same S?N ratio as the signal leaving the
specimen.)

In fact, on all of a large number of different model instruments, the
digitally recorded signal was noisier than it should have been (the
best was 2x worse, the worst almost 1000x worse!!). Clearly some
particular microscope models were much worse than others. A factor of
10 converts to having to use either 10x more beam current or 10x
longer scan time to record the same image as would have been possible
in analogue/photopraphic mode.

At the time of this publication, it wasn't clear how much of the
discrepancy was due to poor design of the SE detector and how much
due to differences in digitization circuitry.

My own opinion is that digitization was the cause of most of the
instrument-to-instrument variation and poor detector design was the
reason that none of the instruments really came close to theoretical
performance.

These results were published in Scanning Volume 18-8, November (1996)
Measuring the Performance of Scanning Electron Microscope Detectors
D.C. Joy, C.S. Joy, R.D. Bunn pp 533.

It is a fascinating paper that shows how to measure a reproduceable
and significant difference between instruments that is at least as
important as any of the "Specs" that they are usually sold on the
basis of.

We can hope that, in the intervening time, manufacturers have
responded to the problem and that if the test were carried out today,
the differences would be less. Or alternatively, we can follow Joy
protocol and test it for ourselves.

Cheers,

Jim P.
--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
3D Microscopy of Living Cells Course, June 10 - 20, 2002, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2002


From daemon Mon Feb 25 23:35:15 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Mon, 25 Feb 2002 23:25:42 -0600
Subject: Tardigrades in a e-sem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Willem Erasmus:

The program Electron Flight Simulator can do the job for you. You can put
any elements together and calculate the density of the mixture and program
will solve the simulation. It can work on simulation of bulk, film layers
on substrate, particles on top and variable presure simulation. In my SEM
lab, it is tied to ISIS-300 EDS system (I do not know if you can run it on
regular computer).
I have no personal interest with Electron Flight Simulator.

Thanks
Zhiyu Wang
Maxtor Corp.



----- Original Message -----
} From: "White, Woody N." {nwwhite-at-mcdermott.com}
To: "'Erasmus, Willem (WJ)'" {willem.erasmus-at-sasol.com} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Monday, February 25, 2002 6:00 PM


Dear All

We are attempting view Tardigrades in a fei/Phillips e-sem. We were unable
to view them in the active state. They curl up in the microscope. When
the oxygen is removed from their environment they become non-active but
bloated. This is what we want. Since they have a soft exoskeleton they
also collapse easy. Probably use the internal water pressure to maintain
their body volume. Has anyone out there had any success with these
magnificent beasts?
If so, please HELP!


Mr. S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana


Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}


From daemon Mon Feb 25 23:56:18 2002



From: Jay :      myInkjets4sale-at-excite.com
Date: Sun, 24 Feb 2002 10:37:39 +-0800
Subject: Buy Manufacture Direct Imaging Supplies Save 60%-80%

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Mon Feb 25 23:57:33 2002



From: Jay :      myInkjets4sale-at-excite.com
Date: Sun, 24 Feb 2002 10:37:39 +-0800
Subject: Buy Manufacture Direct Imaging Supplies Save 60%-80%

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At {a href="http://www.tonerbuys.com"} TonerBuys.com {/a} , the Internet’s premier source for your
printing needs, we recognize that the economy isn’t doing as well
as we’d hoped. Everyone is pinching pennies, scrimping, and saving
where they can.

That’s why we’ve sent you this email--to help you save money, by purchasing
from the only company on the web which is truely manufacture direct.

{a href="http://www.tonerbuys.com"} TonerBuys.com {/a} lets you buy toner or inkjet for your printer at
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lower price than any superstore or Internet store. Take the following
for example:

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We carry all major brands including Hewlett Packard, Canon, Lexmark,
Brother, and Xerox.

But don’t let just our low prices and extensive selection convince you to
shop at TonerBuys.com. In this uncertain economy, being careful about
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From daemon Tue Feb 26 01:25:01 2002



From: =?iso-8859-1?Q?Dal=E9ne?= Josling :      djosling-at-op.up.ac.za
Date: Tue, 26 Feb 2002 09:15:44 +0200
Subject: Ron Anderson found

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good day

I've found Ron's new e-mail address.
Thanks to everyone who responded.

Daléne Josling





From daemon Tue Feb 26 01:45:46 2002



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Date: Tue, 26 Feb 2002 02:35:21 -0500
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Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Tue Feb 26 02:12:28 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Tue, 26 Feb 2002 09:04:49 +0100
Subject: Nyquist and spatial resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have recently read some messages about the Nyquist sampling rate. To
know the appropriate sampling rate for a given CCD-camera on a
microscope I have made a table in which the N.A. of an objective lens
can be related to the necessary magnification needed to sample the image
on a CCD-grid to satisfy the Nyquist sampling rate.

http://ourworld.compuserve.com/homepages/pvosta/pcrnyq.htm

Although sampling at the Nyquist rate will give you nice images, it is
not yet sufficient to give you "reliable" quantitative results in
mesaurements! The Coefficient of Variation (C.V.) in your measurements
decreases with the increase of the diameter of the object as it is
projected on your CCD-array. There are a few very nice publications from
Prof. Ted Young (T.U.Delft, the Netherlands) on this subject. As a
general rule I take that an object (diameter) should cover at least 20
pixels on your CCD-grid to bring the C.V. down to a reasonable level.

Some references:

Ian T. Young,
Not just pretty pictures: Digital quantitative microscopy,
Proc. Royal Microscopical Society, 1996, 31(4), pp. 311-313.

Ian T. Young,
Quantitative Microscopy,
IEEE Engineering in Medicine and Biology, 1996, 15(1), pp. 59-66.

Ian T. Young,
Sampling density and quantitative microsocopy
Analytical and Quantitative Cytology and Histology, vol. 10, 1988, pp.
269-275

These rules are applicable to 2D microscopy, but they can be extended to
confocal microscopy. The difference in sampling in the Z-dimension on a
confocal microscope will probably relate different to this appropriate
sampling density compared to the XY-dimension.

Best regards,

Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/


From daemon Tue Feb 26 03:03:33 2002



From: Hasidin Abd. Rashid :      hasidin-at-forr.upm.edu.my
Date: Tue, 26 Feb 2002 16:50:46 +0800
Subject: LM Need help on wood slide

Contents Retrieved from Microscopy Listserver Archives
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Dear Listmembers,
I would like to know how to soften hardwood for slide preparation. I would
like to share the techniques of softening, sectioning (sliding microtome),
staining and mounting. Anyone want to share please email me.

Regards
Hasidin A. R.
Wood Anatomy Lab
Faculty of Forestry
Universiti Putra Malaysia
43400 Serdang Selangor
Malaysia.



From daemon Tue Feb 26 09:05:43 2002



From: gary.m.brown-at-exxonmobil.com
Date: Tue, 26 Feb 2002 08:55:52 -0600
Subject: Re: image management software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Anton-Jan,

We do not use such a program here. My only experience (albeit very limited)
is with two programs: Aldos "Fetch" and "Quartz PCI". Gatan promotes
(promoted?) Fetch. We have at least one copy. Quartz PCI, the 3rd party
imaging and image manipulation software marketed by Hitachi, contains an
archiving software.

My opinion is that any archiving software requires more up-front work than
it is worth. We choose, for the most part, to keep good records on the
sample types pertaining to each work request and locate images based on
this information. Thus, we tend to spend our time ensuring that we can
locate our archived files. The LabLan has become very useful toward this
purpose.

Another source to check is the microscopy list server
www.msa.microscopy.com/MicroscopyListserver. One can search their
discussion threads for information on a variety of topics. One should note
that, for the most part, the comments on the list server are opinion.
Remember that opinions are like noses: everyone has one and my having one
is perfectly obvious to everyone else.

Good luck and give my best to Johan and the rest of the MCM gang.

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



Alistair D
Westwood To: Gary M Brown/Baytown/ExxonMobil-at-xom, Jerry
W Ball/Baytown/ExxonMobil-at-xom, Sandra M
Wapp/Baytown/ExxonMobil-at-xom, David W
02/26/02 06:55 Abmayr/Baytown/ExxonMobil-at-xom
AM cc:
Subject: image management software




Folks,

Any comments on Anton-Jan's email.

Ali

Alistair D. Westwood
Team Leader - Microscopy & Surface Science
Materials Characterization Lab - Polymer Science Division
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, TX 77520

Ph: (281) 834-5741
Fax: (281) 834-1793
----- Forwarded by Alistair D Westwood/Baytown/ExxonMobil on 02/26/02 06:54
AM -----

Anton-Jan Bons
To: Alistair D
Westwood/Baytown/ExxonMobil-at-xom, Mark M
02/26/02 01:14 Disko/East-US/ExxonMobil-at-xom
AM cc: Johan Stuyver/Benelux/ExxonMobil-at-xom, Marc
H Anthonis/Benelux/ExxonMobil-at-xom
Subject: image management software




Mark, Ali,
We are looking for a software package to manage our microscopy images. We
have PhotoShop, but that's not ideal for batch file conversions, browsing
through large numbers of images, etc. We have a demo version of LView Pro (
http://www.lview.com/AboutLViewPro.htm) which looks very convenient; it's
only 50$ but of course we canot order it just like that... Do you use
software for image management? Do you have any suggestions? It would be
best if we all use the same software.

Thanks. Regards,
Anton-Jan Bons
ExxonMobil Chemical - European Technology Center
Hermeslaan 2, B-1831 Machelen, Belgium
tel: +32 2 722 2838, fax: +32 2 722 2461







From daemon Tue Feb 26 09:58:31 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 26 Feb 2002 10:48:27 -0500
Subject: RE: HPF of leaf tissue

Contents Retrieved from Microscopy Listserver Archives
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Morning Bob,
I hope this is on point, but as a zoologist, I would like to know
how the mesophyl (?-no elementary bio book to check on leaves anymore) holds
up at 30K psi? You may have the same problem with a leaf as I might have
with a piece of mammalian lung. At 30K psi I can only imagine that the
piece of lung would simultaneously be frozen and compressed - to me, that
means, destroyed.
Most of this has been imagined, so I won't spend any more time on
it. Interestingly, the only botany book I have at the moment in my library
is by Johansen, D.A, Plant Embryology, 1950 (Cycads to Anthophyta???-
Spermatophyta (of the time?)), and, of course, no leaves in it.

Regards,

Fred Monson

} ----------
} From: Bob Wise
} Sent: Monday, February 25, 2002 12:39 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: HPF of leaf tissue
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Has anyone out there had any success with high pressure
} freezing/freeze substitution of plant leaves for TEM examination? If
} so, please contact me as I haven't.
}
} Bob
}
} --
} Robert R. Wise, Ph.D.
} Associate Professor of Plant Physiology
} Department of Biology and Microbiology
} University of Wisconsin Oshkosh
}
} On sabbatical from Sept. 2001 to Aug. 2002 at UW Madison
} Botany Department
} B217 Birge Hall
} 430 Lincoln Drive
} Madison, WI 53706
} (608) 262-4288 (phone)
} (608) 262-7509 (fax)
} wise-at-uwosh.edu
} http://www.wisc.edu/biotron/Sharkey/
} http://www.uwosh.edu/departments/biology/wise/wise.html
}
}


From daemon Tue Feb 26 11:08:26 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 26 Feb 2002 09:00:33 -0800
Subject: Re: Joel SEM, riggers needed to move NJ to OH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear David,
You might have some luck contacting a piano moving company, if you don't
have a company that speciallizes in moving hi-tech equipment. The piano
movers are used to getting heavy, delicate instruments out of awkward places.
At 04:25 PM 2/25/2002 -0500, you wrote:
} ------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line
Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.
}
} Greetings,
}
} I needed a company to move a Joel SEM with EDX from central New Jersey to
Cleveland Ohio.
}
} The Cleveland Museum of Natural History has had an offer to have a Joel
SEM donated. The catch is that it is in the basement of a consultants
house. Does anyone know of a company in the Ohio-Pennsylvania-New Jersey
(New York?) area who is, at the least, capable of hoisting it out of his
basement and onto a truck? We may also be interested in delivery and
re-assembly and alignment.
}
} Thank you for any suggestions,
} Dr. David Saja, Geologist
} dsaja-at-cmnh.org
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Feb 26 11:13:15 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 26 Feb 2002 09:08:26 -0800
Subject: Re: LM Need help on wood slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Hasidin,
The time I prepared mahogany for SEM examination, I softened it in hot or
boiling water for a while (half an hour?), then sliced it with a razor blade.
At 04:50 PM 2/26/2002 +0800, you wrote:
}
} Dear Listmembers,
} I would like to know how to soften hardwood for slide preparation. I would
} like to share the techniques of softening, sectioning (sliding microtome),
} staining and mounting. Anyone want to share please email me.
}
} Regards
} Hasidin A. R.
} Wood Anatomy Lab
} Faculty of Forestry
} Universiti Putra Malaysia
} 43400 Serdang Selangor
} Malaysia.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Feb 26 11:56:00 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Mon, 25 Feb 2002 13:47:58 -0500
Subject: Active or Passive Frame Grabber for analogue SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What was supposed to be a simple question has produced
discussion that goes well beyond what I had expected or what I
have the expertise to be able to deal with. I am passing some of
the postings on to my colleagues and we are chewing on
everything as we try to decide what to do with our old SEM. At
least it is essentially new, having sat in a lab for 16 years while
politics kept it in mothballs.
The great expense for us to upgrade from the Polaroid camera to
digital means that we have to be careful what we do.

Greg






From daemon Tue Feb 26 11:56:00 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Tue, 26 Feb 2002 11:35:03 -0600
Subject: Suggestions for LM digital capture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listies,

We are going to purchase a digital capture system for LM and dissection
microscope in our histolab. We want to hook it up to a Mac if possible. We
don't want to spend a million dollars but we do want something with good
resolving abilities and user friendly, is as always, a big plus.

We been having problems getting through to vendors.

Thanks again,
Tim Quinn
University of Kansas
Research Assistant
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu


From daemon Tue Feb 26 12:54:08 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 26 Feb 2002 10:45:30 -0800
Subject: Nitrile gloves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Various components of embedding resins present a lab safety problem; many
are allergenic and some are potentially carcinogenic. Gloves are the usual
precaution, but there's a lot of variation in the protection that they
provide, and using the wrong kind can give a very false sense of security.
I posted this on the list some time ago:

} Tobler & Freiburghaus recommend bulky, clumsy, expensive "4H" gloves for
} } methacrylates [J. Microscopy 160:291-298(1990)], with latex in 2nd place
} & } vinyl 3rd. Ringo, Read, & Cota-Robles [J.E.M. Technique
} 1:417-418(1984)] found } clumsy, cheap polyethylene much better than latex
} in resistance to epoxy } monomers, with vinyl again a poor 3rd. I've heard
} comments that "nitrile is } good", but I haven't found any data yet.

Several listers suggested various nitrile glove manufacturers; I contacted
them, but none could provide info for the monomers that we use. The CDC has
a useful glove chart at http://www.cdc.gov/od/ohs/manual/pprotect.htm. But
all that's listed there is methyl methacrylate (nitrile is poorer than
latex). Does anyone have any data? Please don't post "just don't spill"
comments; safety officers aren't impressed with that attitude.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Feb 26 12:54:45 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 26 Feb 2002 08:49:20 -1000 (HST)
Subject: Re: Suggestions for LM digital capture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Tim-

I believe the Optronics Magnafire and Magnafire SP digital cameras work on
Mac as well as PC. They were demonstrated here a couple of weeks ago, and
both were capable of getting great images on both a compound scope and a
dissecting scope. In fact, histo slides on the Olympus stereo scope looked
fantastic! The software for the Magnafire SP was easy and
intuitive. Contact Optronics or find a distributor on the Web. Olympus is
also a distributor, I think.

} We are going to purchase a digital capture system for LM and dissection
} microscope in our histolab. We want to hook it up to a Mac if possible. We
} don't want to spend a million dollars but we do want something with good
} resolving abilities and user friendly, is as always, a big plus.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Tue Feb 26 13:25:55 2002



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Tue, 26 Feb 2002 13:57:13 -0500
Subject: re: JEOL SEM, riggers needed to move NJ to OH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear David,

You can try to call JEOL directly. Perhaps they will do it for you or could
make recommendations. I only have their central New Jersey service dept.
phone number...(732) 254-5220. Hopefully they will be able to help.

Also, "Allied Van Lines, Inc." has a Special Products Division called
"Electronics Van Inc.". They moved our TEM scope from Massachusetts to NJ.
Their number is (408) 615-1880. Allied's main number is (630) 717-3000.
Allied will not install the scope. You will still need to contact JEOL or
another SEM service company for the installation.

I hope this is helpful. Good Luck.

Jackie Garfield
Lifecell Corp.



From daemon Tue Feb 26 14:26:53 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Tue, 26 Feb 2002 15:18:40 -0500
Subject: Re: tem service on the east coast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ken,
there really isn't any need to be so defensive. i offered my openion on the
subject based on over 20 years in the field. your is biased around a vested
need to keep you and your family fed. which i find no fault in.
i have seen the results of to many companies trying to provide service on
instruments they shouldn't even be attempting. as for the keyboard, it was a
joke. i think most of those that read it got it. well with one exception.
actually it was a laptop with the keys a bit to close together for my rather
large fingers.


------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} John,
} So glad you keep such an open mind. The sticky keyboard is also the
} fault of third party service, right? Thought so.
}
} DISCLAIMER: I have a vested interest in providing high quality service
} to users of SEMs. It's how I make an honorable living and take care of
} my family while helping a lot of wonderful people do THEIR jobs better.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service since 1981
} Delta, PA
}
} "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } take it from someone that has been around em for over 20 years. it is well
woth
} the extra money for the piece of mind that comes from having a service
contract
} with the the compnay you bought your scope from. it may be less expensive
to go
} with a private company like presto, however the costs down the road in
parts,
} if they can be found and in down time, likly to be longer. like i always say
sti
} ck with what you know. companies like philips and jeol are know quanites and
wil
} l be around for a long time.
} } just my nickels worth. you know the price of inflation and my experience
leads
} me to charge more...
} } john
} } all typos are the result od a sticky keyboard.
} }
} }
}
}
}
}



From daemon Tue Feb 26 14:57:38 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 26 Feb 2002 13:55:09 -0700
Subject: Re: image management software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I didn't expect of my quick comment to generate such a feedback. So, I took
a liberty to comment more on this subject. My apologies to the list,
shall anybody find this boring.

} Yes, it can drive the
} beam, but rarely better than the original SEM hardware which you will
} still
} need, incidentally for correcting stig, alignments, etc.

Only modern SEM can compete with the PC or MAC controlled interface in
driving the beam "better". Modern SEM is an unlikely candidate for such
upgrade- all this is about practicality. Further, scan circuits and scan
coils of the SEM do not participate in the beam stigmation. This part is
irrelevant to the subject. Same
applies to beam alignment with respect to the center of the optical column.
Alignment, focus, and stigmation will be done in exactly the same way as
they were done before the active or passive system installation.
Scan rotation and tilt correction (for the most time), are the only circuits
relevant to the scan, which will still participate in the process (outside
of alignment and stigmation), after the
active system is installed. These circuits also do not control SEM scan
generator.

} What he says about
} EDS is partially true but most EDS systems (including older ones) come
} with
} beam control packages. The advantage of getting the beam control from the
} EDS system is that they provide integrated software to do digital mapping,
} linescans, in addition to electron imaging.

EDS system with the beam control package is an active system. With all
advantages of such system. So simple. And again, about practicality- older
EDS mapping system requires multiple connections with SEM, and complex
hardware. Modern active system is all software, both image and EDS
spectra/maps, with simple PCI card and interface box. Much more reliable.

} None of the second part of Feingold's response is accurate. Slower dwell
} times generally do not improve the image for two reasons: First, is that
} you
} tend to build up charging and/or contamination on a stationary spot (are
} you
} familiar with the dark square left behind by the raster?).

Slow scan with high beam dwell time helps to record high resolution images.
That hidden agenda calls for the use of a smaller spot size. That means low
beam current, low signal, high noise, and high integration time. Low beam
current makes for slower charge/contamination grow. Why do a slow scan with
high beam current?

} Secondly, all
} SEMs have a stage drift factor from signififcant to severe. Sitting on a
} stationary spot will actually result in a record of the stage drift. This
} is
} why the EDS companies provide elaborate drift compensation programs with
} their digital beam control packages.

Contamination and stage drift must be repaired, or at least reduced, if they
interfere with your
application. Otherwise don't worry. SEM users and manufacturers may
disagree with the statement (outside the spelling) "all
SEMs have a stage drift factor from signififcant to severe", I certainly
do. Many SEMs have stage lock for critical applications.

} Is Feingold trying to sell an active system? I didn't know there were any
} left on the market.

We are not selling active nor passive systems, however I install and service
both, as well as various
SEMs/TEMs. Vendors of the active systems, just of the top of my head:
http://www.emispec.com/Main/index.html , www.ixrfsystems.com ,
http://www.4pi.com/ . I am sure
there are others- look at
http://www.amc.anl.gov/Docs/NonANL/ComSites.html#Instruments .

In order to keep balance in Nature- vendors of the passive systems:
http://www.gwelectronics.com/ , www.2spi.com ,
http://www.orionmicroscopy.com/ . There a others too. As always, MSA web
site has many useful links.

Noise generated by the digital signal processing is the separate issue.
Prof. James
Pawley addressed that - excellent posting. That noise will be generated by
both active and passive systems. (The following may seem off subject, but I
couldn't help brining this example.) This is one of the reasons why class A
vacuum tube amplifier (potentially capable of) reproducing better quality
sound, than modern digital audio system. But practicality dictates digital
processing, in most instances. Just think about skills and time (number of
attempts) required in order to record best possible image by a CRT/Polaroid
combination, as compared with PC based system (especially for students). And
then scanning the negatives, etc. My
initial comment was about practical - off-the-shelf - solution. Passive
system is economical, active system has more capabilities. Image quality
will be similar in both systems, unless operator pushes SEM to the limit.
When he does, extra capabilities of an active system may come handy.

CRT/Polaroid film recording is certainly a bottleneck of image resolution.
Different vendors addressed that in different ways in the past.
Electromagnetic focus CRT (ISI) is better than electrostatic one. Rodenstock
lenses (Cambridge Stereoscan) is better than Polaroid lenses. And so on.

To Mary Mager- thank you for the correction. I was not aware of EDS mapping
capabilities of the passive Quartz PCI system- can you post a link?

Cheers.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Ascend_jcr {ascend_jcr-at-att.net}
To: Net Gbarclay-at-Trinidad. {gbarclay-at-trinidad.net}
Cc: microscopy. com Microscopy-at-sparc5. {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, February 24, 2002 1:32 PM


Gary,

you get from an image database what you put in. If you just use it as a
replacement of the normal file structure, there is probably more pain than
gain. On the other hand, if you sit down and put some effort into it at the
beginning, like defining what the keyfields are you want to use for later
searches, how the keyfields are supposed to be used, what information you
can in automatically or what information you want to "force" the user to put
in, it can become a very powerful tool indeed.

Our analySIS software contains an embedded database, and we have users who
produce 10s of thousands of images each year and they are happily using the
database. In addition, this opens the possibility to allow other people to
search and download images through the internet. We not only keep image data
in our database but other data as well (EDS data, EELS data, or Word files,
Excel, etc...) so you can also use it as a project database.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "gary.m.brown-at-exxonmobil.com"-at-sparc5.microscopy.com
[mailto:"gary.m.brown-at-exxonmobil.com"-at-sparc5.microscopy.com]
Sent: Tuesday, February 26, 2002 7:56 AM
To: alistair.d.westwood-at-exxonmobil.com
Cc: david.w.abmayr-at-exxonmobil.com; jerry.w.ball-at-exxonmobil.com;
sandra.m.wapp-at-exxonmobil.com



Anton-Jan,

We do not use such a program here. My only experience (albeit very limited)
is with two programs: Aldos "Fetch" and "Quartz PCI". Gatan promotes
(promoted?) Fetch. We have at least one copy. Quartz PCI, the 3rd party
imaging and image manipulation software marketed by Hitachi, contains an
archiving software.

My opinion is that any archiving software requires more up-front work than
it is worth. We choose, for the most part, to keep good records on the
sample types pertaining to each work request and locate images based on
this information. Thus, we tend to spend our time ensuring that we can
locate our archived files. The LabLan has become very useful toward this
purpose.

Another source to check is the microscopy list server
www.msa.microscopy.com/MicroscopyListserver. One can search their
discussion threads for information on a variety of topics. One should note
that, for the most part, the comments on the list server are opinion.
Remember that opinions are like noses: everyone has one and my having one
is perfectly obvious to everyone else.

Good luck and give my best to Johan and the rest of the MCM gang.

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com




Alistair D

Westwood To: Gary M
Brown/Baytown/ExxonMobil-at-xom, Jerry
W Ball/Baytown/ExxonMobil-at-xom,
Sandra M
Wapp/Baytown/ExxonMobil-at-xom,
David W
02/26/02 06:55 Abmayr/Baytown/ExxonMobil-at-xom

AM cc:

Subject: image management
software





Folks,

Any comments on Anton-Jan's email.

Ali

Alistair D. Westwood
Team Leader - Microscopy & Surface Science
Materials Characterization Lab - Polymer Science Division
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, TX 77520

Ph: (281) 834-5741
Fax: (281) 834-1793
----- Forwarded by Alistair D Westwood/Baytown/ExxonMobil on 02/26/02 06:54
AM -----


Anton-Jan Bons

To: Alistair D

Westwood/Baytown/ExxonMobil-at-xom,
Mark M
02/26/02 01:14 Disko/East-US/ExxonMobil-at-xom

AM cc: Johan
Stuyver/Benelux/ExxonMobil-at-xom, Marc
H Anthonis/Benelux/ExxonMobil-at-xom

Subject: image management
software





Mark, Ali,
We are looking for a software package to manage our microscopy images. We
have PhotoShop, but that's not ideal for batch file conversions, browsing
through large numbers of images, etc. We have a demo version of LView Pro (
http://www.lview.com/AboutLViewPro.htm) which looks very convenient; it's
only 50$ but of course we canot order it just like that... Do you use
software for image management? Do you have any suggestions? It would be
best if we all use the same software.

Thanks. Regards,
Anton-Jan Bons
ExxonMobil Chemical - European Technology Center
Hermeslaan 2, B-1831 Machelen, Belgium
tel: +32 2 722 2838, fax: +32 2 722 2461







From daemon Tue Feb 26 15:30:19 2002



From: Yves Giroux :      ygiroux-at-istar.ca
Date: Tue, 26 Feb 2002 16:22:22 -0800
Subject: Fwd: RE: image management software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi NESTOR'

I'm getting very tired of those "promotional-type" answers
(this listserver had, if I remember well, some rules against this).

We also at HITACHI have a very powerful Digital Image Management System
(including very sophisticated Database) called PCI (by Quartz Imaging) but
we are NOT using this listserver to promote it

Yves Giroux
Nissei Sangyo Canada





From: Yves Giroux :      ygiroux-at-istar.ca
Date: Tue, 26 Feb 2002 16:22:22 -0800
Subject: Fwd: RE: image management software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
}
} Anton-Jan,
}
} We do not use such a program here. My only experience (albeit very limited)
} is with two programs: Aldos "Fetch" and "Quartz PCI". Gatan promotes
} (promoted?) Fetch. We have at least one copy. Quartz PCI, the 3rd party
} imaging and image manipulation software marketed by Hitachi, contains an
} archiving software.
}
} My opinion is that any archiving software requires more up-front work than
} it is worth. We choose, for the most part, to keep good records on the
} sample types pertaining to each work request and locate images based on
} this information. Thus, we tend to spend our time ensuring that we can
} locate our archived files. The LabLan has become very useful toward this
} purpose.
}
} Another source to check is the microscopy list server
} www.msa.microscopy.com/MicroscopyListserver. One can search their
} discussion threads for information on a variety of topics. One should note
} that, for the most part, the comments on the list server are opinion.
} Remember that opinions are like noses: everyone has one and my having one
} is perfectly obvious to everyone else.
}
} Good luck and give my best to Johan and the rest of the MCM gang.
}
} Gary M. Brown
} ExxonMobil Chemical Company
} Baytown Polymers Center
} 5200 Bayway Drive
} Baytown, Texas 77520-2101
} phone: (281) 834-2387
} fax: (281) 834-2395
} e-mail: Gary.M.Brown-at-ExxonMobil.com
}
}
}
}
} Alistair D
}
} Westwood To: Gary M
} Brown/Baytown/ExxonMobil-at-xom, Jerry
} W Ball/Baytown/ExxonMobil-at-xom,
} Sandra M
} Wapp/Baytown/ExxonMobil-at-xom,
} David W
} 02/26/02 06:55 Abmayr/Baytown/ExxonMobil-at-xom
}
} AM cc:
}
} Subject: image management
} software
}
}
}
}
}
} Folks,
}
} Any comments on Anton-Jan's email.
}
} Ali
}
} Alistair D. Westwood
} Team Leader - Microscopy & Surface Science
} Materials Characterization Lab - Polymer Science Division
} ExxonMobil Chemical Company
} Baytown Polymers Center
} 5200 Bayway Drive
} Baytown, TX 77520
}
} Ph: (281) 834-5741
} Fax: (281) 834-1793
} ----- Forwarded by Alistair D Westwood/Baytown/ExxonMobil on 02/26/02 06:54
} AM -----
}
}
} Anton-Jan Bons
}
} To: Alistair D
}
} Westwood/Baytown/ExxonMobil-at-xom,
} Mark M
} 02/26/02 01:14 Disko/East-US/ExxonMobil-at-xom
}
} AM cc: Johan
} Stuyver/Benelux/ExxonMobil-at-xom, Marc
} H Anthonis/Benelux/ExxonMobil-at-xom
}
} Subject: image management
} software
}
}
}
}
}
} Mark, Ali,
} We are looking for a software package to manage our microscopy images. We
} have PhotoShop, but that's not ideal for batch file conversions, browsing
} through large numbers of images, etc. We have a demo version of LView Pro (
} http://www.lview.com/AboutLViewPro.htm) which looks very convenient; it's

} only 50$ but of course we canot order it just like that... Do you use
} software for image management? Do you have any suggestions? It would be
} best if we all use the same software.
}
} Thanks. Regards,
} Anton-Jan Bons
} ExxonMobil Chemical - European Technology Center
} Hermeslaan 2, B-1831 Machelen, Belgium
} tel: +32 2 722 2838, fax: +32 2 722 2461
}


From daemon Tue Feb 26 15:39:22 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 26 Feb 2002 18:23:51 -0400
Subject: Sectioning buds in agarose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
A protocol for in-well PCR on FAA fixed arabidopsis buds
includes "microtoming" a block of the infloresence in 5% agarose.
I've already tried a paraffin microtome on a cooled sample and a
cryostat but am unable to obtain sections of consistent thickness (40
um). Any ideas or advice is most welcome.
Rosemary
--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Tue Feb 26 15:50:15 2002



From: PESTOEM-at-aol.com
Date: Tue, 26 Feb 2002 16:44:30 EST
Subject: J.Hoffa response & his sticky keyboard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


His experience eith other service providers is unusual, our contracts
include all spare parts and the response time is mostly faster than the
manufacturers.
Peter Stolzenberg, Pesto Inc.


From daemon Tue Feb 26 17:00:00 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 26 Feb 2002 17:52:25 -0500
Subject: Fwd: RE: image management software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I read your response and then read the response that prompted it and I don't think that the vendor went over the line. I thought that the first paragraph was a good response with respect to the benefits of using a database structure. It was certainly within the scope of the topic.

His second paragraph identified it as a product that they are selling (within the rules of the Listserver) and told of the integration that their software had with other data that comes from a microscope such as EDS and EELS spectra. That is certainly within the scope of today's trend in integrating data from an instrument. One only needs to talk to the reps of all of the microscope manufacturers as well as third party manufacturers of Imaging/EDS digital systems. The remarks that he made with respect to the advantages of their software addresses points in the discussion. In addition, his response favored the use of a database management system and did not tout theirs as better than yours. In fact, if one knows the capabilities of PCI as was demonstrated at the Microscopy shows in the Hitachi booths, his arguments do not at all distract from that product.

Let me state that I am not a user of either Quartz PCI or analySIS but that I have had demos on both. I use a Shareware program called ThumbsPlus for my images, but I am guilty of the points that were made about not being as conscientious about putting the information into the database.

In my opinion, there was no violation of the rules of the Listserver and I agreed with the points that he was making. We just need to relax a little. I am making this plea because I was once "bitten" by a vendor who complained to Nestor that I was too enthusiastic in endorsing one company's product perhaps at the expense of theirs, where I had no commercial interest and that fact was known to the vendor. Where appropriate, Nestor steps in a makes personal comments to individuals when he thinks that they overstep the bounds. I would ask everyone to make certain that a lister's response was intended to "Tick" them off or "Make them tired" before going overboard. Just re-read a response first and think it over before criticizing too harshly. This is a valuable resource with people that are truly interested in helping their colleagues when they can and I hate reading the types of responses that prompted me to respond to this one.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."


-----Original Message-----
} From: Yves Giroux [mailto:ygiroux-at-istar.ca]
Sent: Tuesday, February 26, 2002 7:22 PM
To: Microscopy-at-sparc5.microscopy.com


Hi NESTOR'

I'm getting very tired of those "promotional-type" answers
(this listserver had, if I remember well, some rules against this).

We also at HITACHI have a very powerful Digital Image Management System
(including very sophisticated Database) called PCI (by Quartz Imaging) but
we are NOT using this listserver to promote it

Yves Giroux
Nissei Sangyo Canada





From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 26 Feb 2002 17:52:25 -0500
Subject: Fwd: RE: image management software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
}
} Anton-Jan,
}
} We do not use such a program here. My only experience (albeit very limited)
} is with two programs: Aldos "Fetch" and "Quartz PCI". Gatan promotes
} (promoted?) Fetch. We have at least one copy. Quartz PCI, the 3rd party
} imaging and image manipulation software marketed by Hitachi, contains an
} archiving software.
}
} My opinion is that any archiving software requires more up-front work than
} it is worth. We choose, for the most part, to keep good records on the
} sample types pertaining to each work request and locate images based on
} this information. Thus, we tend to spend our time ensuring that we can
} locate our archived files. The LabLan has become very useful toward this
} purpose.
}
} Another source to check is the microscopy list server
} www.msa.microscopy.com/MicroscopyListserver. One can search their
} discussion threads for information on a variety of topics. One should note
} that, for the most part, the comments on the list server are opinion.
} Remember that opinions are like noses: everyone has one and my having one
} is perfectly obvious to everyone else.
}
} Good luck and give my best to Johan and the rest of the MCM gang.
}
} Gary M. Brown
} ExxonMobil Chemical Company
} Baytown Polymers Center
} 5200 Bayway Drive
} Baytown, Texas 77520-2101
} phone: (281) 834-2387
} fax: (281) 834-2395
} e-mail: Gary.M.Brown-at-ExxonMobil.com
}
}
}
}
} Alistair D
}
} Westwood To: Gary M
} Brown/Baytown/ExxonMobil-at-xom, Jerry
} W Ball/Baytown/ExxonMobil-at-xom,
} Sandra M
} Wapp/Baytown/ExxonMobil-at-xom,
} David W
} 02/26/02 06:55 Abmayr/Baytown/ExxonMobil-at-xom
}
} AM cc:
}
} Subject: image management
} software
}
}
}
}
}
} Folks,
}
} Any comments on Anton-Jan's email.
}
} Ali
}
} Alistair D. Westwood
} Team Leader - Microscopy & Surface Science
} Materials Characterization Lab - Polymer Science Division
} ExxonMobil Chemical Company
} Baytown Polymers Center
} 5200 Bayway Drive
} Baytown, TX 77520
}
} Ph: (281) 834-5741
} Fax: (281) 834-1793
} ----- Forwarded by Alistair D Westwood/Baytown/ExxonMobil on 02/26/02 06:54
} AM -----
}
}
} Anton-Jan Bons
}
} To: Alistair D
}
} Westwood/Baytown/ExxonMobil-at-xom,
} Mark M
} 02/26/02 01:14 Disko/East-US/ExxonMobil-at-xom
}
} AM cc: Johan
} Stuyver/Benelux/ExxonMobil-at-xom, Marc
} H Anthonis/Benelux/ExxonMobil-at-xom
}
} Subject: image management
} software
}
}
}
}
}
} Mark, Ali,
} We are looking for a software package to manage our microscopy images. We
} have PhotoShop, but that's not ideal for batch file conversions, browsing
} through large numbers of images, etc. We have a demo version of LView Pro (
} http://www.lview.com/AboutLViewPro.htm) which looks very convenient; it's

} only 50$ but of course we canot order it just like that... Do you use
} software for image management? Do you have any suggestions? It would be
} best if we all use the same software.
}
} Thanks. Regards,
} Anton-Jan Bons
} ExxonMobil Chemical - European Technology Center
} Hermeslaan 2, B-1831 Machelen, Belgium
} tel: +32 2 722 2838, fax: +32 2 722 2461
}


From daemon Tue Feb 26 17:13:02 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Tue, 26 Feb 2002 15:07:36 -0800 (PST)
Subject: EDX detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I've received a few quotations for EDX detectors and will put together a
package of recommendations to our users. The one's I've narrowed it to
are from EDAX, PGT, or IXRF. They both offer EDX systems for our cold
FESEM Hitachi S5000. I was wondering if anyone has good/bad experiences they
could relate, or suggestions for EDX detectors for this particular SEM.

Thanks for your help.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793




From daemon Tue Feb 26 18:12:54 2002



From: Kim Rensing :      krensing-at-interchange.ubc.ca
Date: Tue, 26 Feb 2002 16:05:28 -0800
Subject: Re: HPF of leaf tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Has anyone out there had any success with high pressure
} freezing/freeze substitution of plant leaves for TEM examination? If
} so, please contact me as I haven't.
}
} Bob

Bob,

We haven't done any HPF on leaf here, but have had great success on cambium
and developing xylem. I think the greatest obstacle would be to fill the
intercellular spaces with cryoprotectant. (See Fred Monson's concerns.) We
use 0.2M sucrose in water for most plant tissues. If you immerse the cut
tissues in cryoprotectant then place them under vacuum several times, you
may have some success.

As to cryosubstitution, the best and easiest medium is (seems to be) 2%
osmium tetroxide in acetone with 8% dimethoxypropane (as water scavenger).
Substitute for at least 5 days for best results. (There are many other
recipes depending on what your ultimate goal is.)

You may also ruin your hard work by adding resin too quickly during
embedding.

If you have any questions, email me directly.

Kim
-------
Kim Rensing Ph.D.
Dept. of Botany, UBC
6270 University Blvd
Vancouver, BC, Canada
V6T 1Z4

phone: 604-822-5223
fax: 604-822-6089



From daemon Tue Feb 26 19:27:53 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 26 Feb 2002 17:24:32 -0800
Subject: Re: Fwd: RE: image management software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Others are promoting it.

Fine. Caveat emptor.

I don't really see that much pushing of one product
over another. What is being discussed are detailed
technical issues. A buyer can use this information
to sort our what is best for them.

The more data a buyer has, the better. Don't
you agree? Or, are you saying that your product
is the only viable option? Of course not. This is
I think, a discussion forum. there are many facets
to discussion.

gary g.


At 04:22 PM 2/26/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Feb 26 20:12:05 2002



From: zaluzec-at-microscopy.com
Date: Tue, 26 Feb 2002 20:03:40 -0600
Subject: Administrivia: Is it Advertising or Not...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Colleagues....

Just to defuse a potential arguement.

In my opinon the latest thread on Image Management Software
was within both the intent and letter of our rules.

Let me remind you all of the nominal rules about the use of the
Listserver with respect to Commerical Products. This
information is taken directly from the FAQ page which you ALL
received a copy of when you subscribed, and is available on-line
on the WWW site., the link to which is on EVERY EMAIL sent
through this Listserver

As Scott W. pointed out. When I notice someone going over the line
they get a warning. If it happens multiple times, they run
the risk of becoming banned from posting messages to the Listserver.

Nestor
Your Friendly Neighborhood SysOp

****************************************************
Exerpts from the Microscopy Listserver FAQ....
****************************************************
------------------------------------
I am a Commerical Manufacturer. Can I participate?
-------------------------------------

YES!

If you are a manufacturer, you are always welcome to observe/join in
any discussion at all times.
We do ask that everyone, please refrain from overt sales pitches and/or
commericalism. If a product which you produce/sell can solve a
problem or answer a
question raised by anyone on this list, then by all means feel free
to say so. Try to be
brief about the product, state the simple facts in a few (short)
sentences and then offer
to continue the discussion with any interested parties offline or
point people to a WWW Site
with detailed information, so that individuals can download/access
the relevant information.
Usually it will be sufficient to just add your phone number and/or
Email address to the end of your
message, and you'll be contacted by anyone that is interested.

Please note that: UNSOLICITED product announcements are advertising
and do not belong in this forum.

Remember, please keep your comments about any product you "sell" to a minimum.

It is not out of line to provide your company name, Email address or
WWW site as part of your
signoff/signature line, at the end of ANY message you post to this system.

This Listserver operates on the honor system with respect to to
posting of advertising,
so please respect these simple ground rules.


------------------------------------------------------------
Can I post a For Sale / Advertisement / Commerical / Marketing Survey
Message(s)?
------------------------------------------------------------

No, that does not fit within the bounds of this discussion forum.

This listserver is not intended to be a Sales/Marketing/Survey mechanism for
organizations, but rather it is an open discussion area about microscopy and
microanalysis problems and solutions.

If you are an organization and have equipment you wish to donate,
(or sell, for nominal cost i.e. no profit) then this is generally an
acceptable posting.
If you are not sure then send a copy of the announcement in question to
Zaluzec-at-MSA.Microscopy.Com and I will give you my opinion. An example of this
type would be an old decommissioned instrument which someone is trying to
give away for removal/shipping costs, that would fit within the
bounds of the purposes of this list.

There is a mechanism to post Commerical NEWS to the Microscopy Community.
If you have something of a purely Commerical Nature and wish to make
it known to the
community you may FREELY use the following WWW site (just follow the
on-line instructions)

http://www.msa.microscopy.com/News/NewsListings.html

If you have surplus equipment you wish to "sell" ( for profit) please use

http://www.msa.microscopy.com/SurplusEquipment


From daemon Tue Feb 26 21:33:07 2002



From: earlw-at-sbcglobal.net :      earlw-at-sbcglobal.net
Date: Tue, 26 Feb 2002 09:00:33 -0800
Subject: Re: Joel SEM, riggers needed to move NJ to OH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I typically move about 15 SEMs per year.
I like to have one source for the SEM move & rigging as I don't want any "finger pointing" if something goes wrong.

The Company that I have had the most sucess with Domestic or International Is:

Fast Forward
Pamela Parsons
(877) 978-6300

They will handle the rigging, crating & move. For dis-assembly of the SEM you may wish to call JEOL or a third party vendor.

Disclaimer: I have no vested interest in Fast Forward other than seeing a good Company be rewarded by recomendations.

Regards,

Earl Weltmer



Original Message:
-----------------
} From: Mary Mager mager-at-interchange.ubc.ca


Dear David,
You might have some luck contacting a piano moving company, if you don't
have a company that speciallizes in moving hi-tech equipment. The piano
movers are used to getting heavy, delicate instruments out of awkward places.
At 04:25 PM 2/25/2002 -0500, you wrote:
} ------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line
Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.
}
} Greetings,
}
} I needed a company to move a Joel SEM with EDX from central New Jersey to
Cleveland Ohio.
}
} The Cleveland Museum of Natural History has had an offer to have a Joel
SEM donated. The catch is that it is in the basement of a consultants
house. Does anyone know of a company in the Ohio-Pennsylvania-New Jersey
(New York?) area who is, at the least, capable of hoisting it out of his
basement and onto a truck? We may also be interested in delivery and
re-assembly and alignment.
}
} Thank you for any suggestions,
} Dr. David Saja, Geologist
} dsaja-at-cmnh.org
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca


--------------------------------------------------------------------
mail2web - Check your email from the web at
http://mail2web.com/ .



From daemon Tue Feb 26 21:33:07 2002



From: earlw-at-sbcglobal.net :      earlw-at-sbcglobal.net
Date: Tue, 26 Feb 2002 09:00:33 -0800
Subject: Re: Joel SEM, riggers needed to move NJ to OH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I typically move about 15 SEMs per year.
I like to have one source for the SEM move & rigging as I don't want any "finger pointing" if something goes wrong.

The Company that I have had the most sucess with Domestic or International Is:

Fast Forward
Pamela Parsons
(877) 978-6300

They will handle the rigging, crating & move. For dis-assembly of the SEM you may wish to call JEOL or a third party vendor.

Disclaimer: I have no vested interest in Fast Forward other than seeing a good Company be rewarded by recomendations.

Regards,

Earl Weltmer



Original Message:
-----------------
} From: Mary Mager mager-at-interchange.ubc.ca


Dear David,
You might have some luck contacting a piano moving company, if you don't
have a company that speciallizes in moving hi-tech equipment. The piano
movers are used to getting heavy, delicate instruments out of awkward places.
At 04:25 PM 2/25/2002 -0500, you wrote:
} ------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line
Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.
}
} Greetings,
}
} I needed a company to move a Joel SEM with EDX from central New Jersey to
Cleveland Ohio.
}
} The Cleveland Museum of Natural History has had an offer to have a Joel
SEM donated. The catch is that it is in the basement of a consultants
house. Does anyone know of a company in the Ohio-Pennsylvania-New Jersey
(New York?) area who is, at the least, capable of hoisting it out of his
basement and onto a truck? We may also be interested in delivery and
re-assembly and alignment.
}
} Thank you for any suggestions,
} Dr. David Saja, Geologist
} dsaja-at-cmnh.org
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca


--------------------------------------------------------------------
mail2web - Check your email from the web at
http://mail2web.com/ .



From daemon Tue Feb 26 21:33:07 2002



From: earlw-at-sbcglobal.net :      earlw-at-sbcglobal.net
Date: Tue, 26 Feb 2002 09:00:33 -0800
Subject: Re: Joel SEM, riggers needed to move NJ to OH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I typically move about 15 SEMs per year.
I like to have one source for the SEM move & rigging as I don't want any "finger pointing" if something goes wrong.

The Company that I have had the most sucess with Domestic or International Is:

Fast Forward
Pamela Parsons
(877) 978-6300

They will handle the rigging, crating & move. For dis-assembly of the SEM you may wish to call JEOL or a third party vendor.

Disclaimer: I have no vested interest in Fast Forward other than seeing a good Company be rewarded by recomendations.

Regards,

Earl Weltmer



Original Message:
-----------------
} From: Mary Mager mager-at-interchange.ubc.ca


Dear David,
You might have some luck contacting a piano moving company, if you don't
have a company that speciallizes in moving hi-tech equipment. The piano
movers are used to getting heavy, delicate instruments out of awkward places.
At 04:25 PM 2/25/2002 -0500, you wrote:
} ------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line
Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.
}
} Greetings,
}
} I needed a company to move a Joel SEM with EDX from central New Jersey to
Cleveland Ohio.
}
} The Cleveland Museum of Natural History has had an offer to have a Joel
SEM donated. The catch is that it is in the basement of a consultants
house. Does anyone know of a company in the Ohio-Pennsylvania-New Jersey
(New York?) area who is, at the least, capable of hoisting it out of his
basement and onto a truck? We may also be interested in delivery and
re-assembly and alignment.
}
} Thank you for any suggestions,
} Dr. David Saja, Geologist
} dsaja-at-cmnh.org
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca


--------------------------------------------------------------------
mail2web - Check your email from the web at
http://mail2web.com/ .



From daemon Tue Feb 26 21:33:07 2002



From: earlw-at-sbcglobal.net :      earlw-at-sbcglobal.net
Date: Tue, 26 Feb 2002 09:00:33 -0800
Subject: Re: Joel SEM, riggers needed to move NJ to OH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I typically move about 15 SEMs per year.
I like to have one source for the SEM move & rigging as I don't want any "finger pointing" if something goes wrong.

The Company that I have had the most sucess with Domestic or International Is:

Fast Forward
Pamela Parsons
(877) 978-6300

They will handle the rigging, crating & move. For dis-assembly of the SEM you may wish to call JEOL or a third party vendor.

Disclaimer: I have no vested interest in Fast Forward other than seeing a good Company be rewarded by recomendations.

Regards,

Earl Weltmer

Original Message:
-----------------
} From: Mary Mager mager-at-interchange.ubc.ca


Dear David,
You might have some luck contacting a piano moving company, if you don't
have a company that speciallizes in moving hi-tech equipment. The piano
movers are used to getting heavy, delicate instruments out of awkward places.
At 04:25 PM 2/25/2002 -0500, you wrote:
} ------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line
Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.
}
} Greetings,
}
} I needed a company to move a Joel SEM with EDX from central New Jersey to
Cleveland Ohio.
}
} The Cleveland Museum of Natural History has had an offer to have a Joel
SEM donated. The catch is that it is in the basement of a consultants
house. Does anyone know of a company in the Ohio-Pennsylvania-New Jersey
(New York?) area who is, at the least, capable of hoisting it out of his
basement and onto a truck? We may also be interested in delivery and
re-assembly and alignment.
}
} Thank you for any suggestions,
} Dr. David Saja, Geologist
} dsaja-at-cmnh.org
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca


--------------------------------------------------------------------
mail2web - Check your email from the web at
http://mail2web.com/ .



From daemon Tue Feb 26 21:54:37 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Wed, 27 Feb 2002 14:52:26 +1100
Subject: RE: HPF of leaf tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bob,

I didn't answer this earlier, as have only tried HPF a couple of times.
The key is, as Fred Monson implies, to fill all air spaces with liquid
first. I've done this by vacuum infiltration of pieces of mature root, but
have not tried leaf pieces. Some people also surround the tissue with some
sort of cryoprotectant, or at least with some (usually fairly viscous)
solution that is less likely to form crystals, like a solution of dextrin
or BSA, for example. You could try vacuum infiltration of the leaf pieces,
either with water or with some aqueous non-osmotic "cryoprotectant".
Good luck, cheers, Rosemary

}
} Morning Bob,
} I hope this is on point, but as a zoologist, I would like to know
} how the mesophyl (?-no elementary bio book to check on leaves anymore) holds
} up at 30K psi? You may have the same problem with a leaf as I might have
} with a piece of mammalian lung. At 30K psi I can only imagine that the
} piece of lung would simultaneously be frozen and compressed - to me, that
} means, destroyed.
} Most of this has been imagined, so I won't spend any more time on
} it. Interestingly, the only botany book I have at the moment in my library
} is by Johansen, D.A, Plant Embryology, 1950 (Cycads to Anthophyta???-
} Spermatophyta (of the time?)), and, of course, no leaves in it.
}
} Regards,
}
} Fred Monson
}
} } ----------
} } From: Bob Wise
} } Sent: Monday, February 25, 2002 12:39 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: HPF of leaf tissue
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Has anyone out there had any success with high pressure
} } freezing/freeze substitution of plant leaves for TEM examination? If
} } so, please contact me as I haven't.
} }
} } Bob
} }
} } --
} } Robert R. Wise, Ph.D.
} } Associate Professor of Plant Physiology
} } Department of Biology and Microbiology
} } University of Wisconsin Oshkosh
} }
} } On sabbatical from Sept. 2001 to Aug. 2002 at UW Madison
} } Botany Department
} } B217 Birge Hall
} } 430 Lincoln Drive
} } Madison, WI 53706
} } (608) 262-4288 (phone)
} } (608) 262-7509 (fax)
} } wise-at-uwosh.edu
} } http://www.wisc.edu/biotron/Sharkey/
} } http://www.uwosh.edu/departments/biology/wise/wise.html
} }
} }


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au




From daemon Wed Feb 27 01:05:29 2002



From: Earl Weltmer :      eweltmer1-at-cox.net
Date: Tue, 26 Feb 2002 09:00:33 -0800
Subject: Re: Joel SEM, riggers needed to move NJ to OH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I typically move about 15 SEMs per year.
I like to have one source for the SEM move & rigging as I don't want any
"finger pointing" if something goes wrong.

The Company that I have had the most sucess with Domestic or International
Is:

Fast Forward
Pamela Parsons
(877) 978-6300

They will handle the rigging, crating & move. For dis-assembly of the SEM
you may wish to call JEOL or a third party vendor.

Disclaimer: I have no vested interest in Fast Forward other than seeing a
good Company be rewarded by recomendations.

Regards,

Earl Weltmer

Original Message:
-----------------
} From: Mary Mager mager-at-interchange.ubc.ca


Dear David,
You might have some luck contacting a piano moving company, if you don't
have a company that speciallizes in moving hi-tech equipment. The piano
movers are used to getting heavy, delicate instruments out of awkward
places.
At 04:25 PM 2/25/2002 -0500, you wrote:
} ------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line
Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.
}
} Greetings,
}
} I needed a company to move a Joel SEM with EDX from central New Jersey to
Cleveland Ohio.
}
} The Cleveland Museum of Natural History has had an offer to have a Joel
SEM donated. The catch is that it is in the basement of a consultants
house. Does anyone know of a company in the Ohio-Pennsylvania-New Jersey
(New York?) area who is, at the least, capable of hoisting it out of his
basement and onto a truck? We may also be interested in delivery and
re-assembly and alignment.
}
} Thank you for any suggestions,
} Dr. David Saja, Geologist
} dsaja-at-cmnh.org
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Feb 27 03:25:22 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Wed, 27 Feb 2002 11:16:32 +0200
Subject: Aluminim evaporated films in grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

One researcher here is doing research on the properties of thin films. Part
of the studies would be to do TEM imaging of the films. What type of
support film can we put on Cu grids for the evaporated film. I had a go
with formvar, but they brake down during evaporation due to the heat of the
plasma. I would like to dissolver the support film if possible afterwards.

Suggestions?


Mr. S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}




From daemon Wed Feb 27 05:19:34 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 27 Feb 2002 06:09:07 -0500
Subject: Support films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Mr. S. H. Coetzee wrote the following:
================================================================
One researcher here is doing research on the properties of thin films. Part
of the studies would be to do TEM imaging of the films. What type of
support film can we put on Cu grids for the evaporated film. I had a go
with formvar, but they brake down during evaporation due to the heat of the
plasma. I would like to dissolver the support film if possible afterwards.
================================================================
There are several possibilities here but it is not clear what you mean by
"during evaporation due to the heat of the plasma".

If Formvar® is not acceptable, for whatever the reasons, you could consider
carbon or if you really are exposing these to a plasma, then SiO2 films.
Information about these films can be found on URL
http://www.2spi.com/catalog/grids/cusctgrd.html However, both films
exhibit a certain degree of granularity on the nanoscale which might be
distracting if not also confusing in the analysis of your micrographs.

Another approach is to pick up the films on a silicon nitride membrane
window grid, see URL
http://www.2spi.com/catalog/instruments/silicon-nitride.html
The window is not going to be hurt by exposure to an oxygen plasma, and it
is completely amorphous and therefore structureless and featureless. It is
also quite electron transparent.

There is a final possibility for solving the problem. You did not mention
what mesh size you are using, but you switch to using 2000 mesh grids and
possibly eliminate the need altogether for the use of any support film or
the more expensive silicon nitride membrane window grids.

Disclaimer: SPI Supplies offers custom coated grids and the other items
mentioned in this posting.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================


From daemon Wed Feb 27 06:04:47 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 27 Feb 2002 12:01:51 +0000 (GMT Standard Time)
Subject: Re: Aluminim evaporated films in grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Coetzee,

We regularly sputter or evaporate thin films onto standard
carbon filmed (no formvar) Cu or Au grids, usually 200
mesh. We have built a small holder to mount the grids into
for ease of handling. Don't put the evaporation source too
close to the grids, I think ours is about 150mm.

Good luck,
Ron

On Wed, 27 Feb 2002 11:16:32 +0200 "Coetzee, Mr S. H
Physics Science" {COETZEES-at-mopipi.ub.bw} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all
}
} One researcher here is doing research on the properties of thin films. Part
} of the studies would be to do TEM imaging of the films. What type of
} support film can we put on Cu grids for the evaporated film. I had a go
} with formvar, but they brake down during evaporation due to the heat of the
} plasma. I would like to dissolver the support film if possible afterwards.
}
} Suggestions?
}
}
} Mr. S. H. Coetzee
} Electron Microscope Unit
} University of Botswana
} Private Bag 0022
} Gabarone
} Botswana
}
} Phone : +267 355 2426
} Mobile: +267 718 96 729
} Fax : +267 585 097
} e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Feb 27 08:06:27 2002



From: JHoffpa464-at-aol.com
Date: Wed, 27 Feb 2002 08:58:13 EST
Subject: Re: J.Hoffa response & his sticky keyboard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


well peter considering you are the compnay i was refering to, then it must have just been one of those weeks for you. peter i have delt with you directly and the truth is i find the manufactures to have much better trained people.
i wasn't going to mention it, but you decided to put your two cents in and get free advertisement on the list server.

in a dept i worked at about 9 years ago, we bought a used 301 from you. you came in installed it got a vacuum and said it was running fine.i come in the next day the scope is un alinged. so i did that within 3 days the scope was down. it took us over a week to get you back to get it up and running. we then called philips and had it put unnder their contract. if you want to respond email me directly.
John Hoffpauir
Thomas Jefferson University


From daemon Wed Feb 27 08:19:48 2002



From: Lou Bustillos :      lbustillos-at-amalab.com
Date: Tue, 26 Feb 2002 15:18:40 -0500
Subject: Re: tem service on the east coast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All information given here is based on experience.

I have been using Pesto, Inc. for the last four years. JEOL was
getting too expensive, especially since I have two scopes. The
response time from Pesto, Inc. has been great and getting replacement
parts has not been a problem.

Lou Bustillos
AMA Analytical Services, Inc.

---------- Original Message ----------------------------------
} From: "John Hoffpauir" {John.Hoffpauir-at-mail.tju.edu}


From daemon Wed Feb 27 08:49:01 2002



From: JHoffpa464-at-aol.com
Date: Wed, 27 Feb 2002 10:15:58 EST
Subject: TRANSWELL MEMBRANES

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-----Message d'origine-----
De: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]


*********************************************
This Email contains Important Information about
the Microscopy Listserver. Please read it all then
SAVE a copy for future reference. - Nestor
****************************************
To: NewSub-at-MSA.Microscopy.Com
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}


has anyone out there had any experience in cutting one micron sections of transwell membranes? a tech in my lab is having trouble getting them flat on a glass slide. he has wrinkles which is unacceptable for publication. i have never worked with them. any advice would be great.
john hoffpauir


From daemon Wed Feb 27 09:36:26 2002



From: Rich Fiore :      rafiore-at-unity.ncsu.edu
Date: Wed, 27 Feb 2002 10:31:10 -0500
Subject: Used RGA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a used RGA for sale? This will be used on a SIMS system
and only needs to go to about 100 AMU.

Rich Fiore
NC State University
Analytical Instrumentation Facility
1010 Main Campus Drive
318 Engineering Graduate Research Ctr., Box 7531
Raleigh, NC 27695
Tel: 919-515-2348
Fax: 919-515-6965
Email: rafiore-at-unity.ncsu.edu



From daemon Wed Feb 27 10:34:30 2002



From: Andrew Ochalski :      AOCHALSK-at-science.uottawa.ca
Date: Wed, 27 Feb 2002 11:27:41 -0500
Subject: Live Cell Imaging: Stage Drift and Shutter Woes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{paraindent} {param} right {/param} {color} {param} 0100,0100,0100 {/param} {/paraindent}

{paraindent} {param} right {/param} Hello all, {/paraindent}

{paraindent} {param} right {/param} {/color} We are attempting to perform time-lapse imaging of GFP-labelled
cells on a Zeiss Axiophot 1 (an upright microscope). There are two
aspects of the system that cause us trouble. The first is our Uniblitz
shutter, which is driven via parallel port by a module that is part of the
Universal Imaging Metamorph v.4.0 suite. The shutter responds to
oscilloscope-generated current pulses to provide open (exposure) times
of 20 ms (as stated in the specs). We require open times of 100-200
ms, but the shortest pulse interval that can be generated through the
parallel cable by any exposure control function in the software is 500-
600 ms. No-one connected with U.I. or the company that sold us our
system can explain why this is the case, and how we might achieve
shorter exposure times. Is there anyone out there with experience or
knowledge that might be brought to bear on this issue? {/paraindent}

{paraindent} {param} right {/param} The second problem is more troubling. Even without a heating
chamber, we experience a small, variable, but problematic downward
drift of the specimen stage. The total drift is ~0.5 -1um per minute
(sometimes more or less), but is enough to scuttle velocity measurements
with our 1.4 NA lens. To solve this, we bought a Prior Optiscan z-axis
focus controller, which meshes a gear with the microscope's coarse
focus gear. We thought this might prevent the subtle stage slippage that
is compromising our observations. No dice. Again, does anyone have an
insight, knowledge or words of wisdom? Thanks in advance. {/paraindent}


{paraindent} {param} right {/param} {color} {param} 0100,0100,0100 {/param} {smaller} . {/paraindent}


{nofill}


From daemon Wed Feb 27 10:34:31 2002



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Wed, 27 Feb 2002 09:25:59 -0700
Subject: RE: Aluminum evaporated films in grids

Contents Retrieved from Microscopy Listserver Archives
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Dear Mr. Coetzee,

If your polymer films are breaking, you might be better off if you first evaporate C on them for stability, then on
the opposite side of the grid deposit your Al. Finally one can remove the carbon/polymer in a proper solvent leaving the
Al film all by itself.
Alternatively, one can deposit the Al films on rocksalt and float the films off in water.

I hope this helps

Jordi

PS.
We have often deposited carbon (not metal) on polymer films without problems, maybe you can place your grids/w.polymer
further away form the Al source to minimize breakage.
----------------------
} Mr. S. H. Coetzee wrote:
}
}
} Dear all
}
} One researcher here is doing research on the properties of thin films. Part
} of the studies would be to do TEM imaging of the films. What type of
} support film can we put on Cu grids for the evaporated film. I had a go
} with formvar, but they brake down during evaporation due to the heat of the
} plasma. I would like to dissolver the support film if possible afterwards.
}
} Suggestions?
}
}
} Mr. S. H. Coetzee
} Electron Microscope Unit
} University of Botswana
} Private Bag 0022
} Gabarone
} Botswana
}
} Phone : +267 355 2426
} Mobile: +267 718 96 729
} Fax : +267 585 097
} e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Feb 27 11:05:26 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 27 Feb 2002 09:00:52 -0800
Subject: Re: Aluminim evaporated films in grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Coetzee,
We use a carbon evaporated film to support thin films and small particles
for our TEM work. First we cast a collodion film on water, then float TEM
grids on it, lift the film with filter paper and dry it. Carbon coat the
collodion on grids and then dissolve the colodion off in a Jaffe washer
filled with chloroform for 48 hours. The resulting amorphous carbon film is
strong and conductive and easily lasts 200 kV in our TEM.
At 11:16 AM 2/27/2002 +0200, you wrote:
} Dear all
}
} One researcher here is doing research on the properties of thin films. Part
} of the studies would be to do TEM imaging of the films. What type of
} support film can we put on Cu grids for the evaporated film. I had a go
} with formvar, but they brake down during evaporation due to the heat of the
} plasma. I would like to dissolver the support film if possible afterwards.
}
} Suggestions?
}
}
} Mr. S. H. Coetzee
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Feb 27 11:15:38 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 27 Feb 2002 09:11:33 -0800
Subject: Re: Active or Passive Frame Grabber for aqnalogue SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Vitatly,
I'm sorry I didn't post a link to the Quartz XOne EDS system. It is:
www.quartzimaging.com. This product is not the same as the Quartz PCI image
capture system and is not assiciated with Hitachi.
Disclaimer: I have been involved with the design and selling of the XOne
system since its inception.
At 03:47 PM 2/26/2002 -0500, you wrote:
} (snip)
} } What he says about
} } EDS is partially true but most EDS systems (including older ones) come
} } with
} } beam control packages. The advantage of getting the beam control from the
} } EDS system is that they provide integrated software to do digital mapping,
} } linescans, in addition to electron imaging.
}
} EDS system with the beam control package is an active system. With all
}
} To Mary Mager- thank you for the correction. I was not aware of EDS mapping
} capabilities of the passive Quartz PCI system- can you post a link?
}
} Cheers.
}
} Vitaly Feingold

Regards,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Feb 27 11:49:38 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Wed, 27 Feb 2002 12:41:50 -0500
Subject: Aluminim evaporated films in grids

Contents Retrieved from Microscopy Listserver Archives
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S.H.
You could use a carbon support film. You can get quite thin using indirect
deposition by evaporation thereby eliminating any contributing structure. A
lacy film may be susceptible to damage as a support for the carbon so a
high mesh grid would be appropriate. We often float films off cleaved mica
used as a substrate although this depends on adhesion and may not be
appropriate for your films.
Good Luck,
Russ Gillmeister
Microscopy
Bldg. 114-42D
Xerox Corp.
800 Phillips Rd.
Webster, NY 14580
(585) 422-5317


-----Original Message-----
} From: Coetzee, Mr S. H Physics Science [mailto:COETZEES-at-mopipi.ub.bw]
Sent: Wednesday, February 27, 2002 4:17 AM
To: Listserver (E-mail)


Dear all

One researcher here is doing research on the properties of thin films. Part
of the studies would be to do TEM imaging of the films. What type of
support film can we put on Cu grids for the evaporated film. I had a go
with formvar, but they brake down during evaporation due to the heat of the
plasma. I would like to dissolver the support film if possible afterwards.

Suggestions?


Mr. S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}




From daemon Wed Feb 27 12:08:16 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 27 Feb 2002 09:59:08 -0800
Subject: Microprobe tracks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Sorry if this is a little off our topic line.

I recently carbon coated some samples for a user who took them to a
microprobe for analysis.

He called to ask about the carbon coating's affect on the appearance of
the sample after analysis.

On a previous run, with a different instrument, he said the beam 'toasted'
the area of analysis and he liked that since it showed exactly where the
beam had been. On the instrument he used here, there was no toasting, so no
visible record of the analytical location.

I don't run the probe, I just do the carbon coating. But I thought someone
out there in microscope land might know what I can tell him. He says the
toasting probe was newer and better than the older one we have here. He
said the beam conditions were similar for the two instruments.

I am curious. What are the toast tracks and does the carbon coating have
anything to do with them?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Wed Feb 27 14:33:15 2002



From: Ladd Research :      sales-at-laddresearch.com
Date: Wed, 27 Feb 2002 15:25:33 -0500
Subject: Re: micrometer

Contents Retrieved from Microscopy Listserver Archives
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Dr. Coetzee,

All plastics will break down from the heat after a period of time. A
carbon substrate will last longer. When we make our carbon substrate we
use carbon and formvar and then dissolve the formvar. If this doesn't
solve your problems contact us we have other options.

John Arnott
--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
FAX: 1-802-660-8859
e-mail: sales-at-laddresearch.com

Quality Since 1955


Yash Agarwal wrote:
}
} Hi,
}
} I am an engineer at Ikonisys Inc. We were looking for a graticule with the
} following requirements
}
} 1. Graticule for Reflected Fluorescence Imaging. (No bright field)
} 1. Graticule slide dimensions - 75mm x 25mm x 1mm.
} 2. Cross pattern Graticule with Micrometer scale of 5mm in 0.05mm divisions.
}
} Could you provide me with the information whether you would have such a
} Graticule?
} If not can it be custom made and if yes what would it cost?
}
} Thank you
}
} Yash Agarwal
} Ikonisys Inc.
} 5 Science Park
} Suite 1000,
} New Haven, CT 06515
} Tel: 203-776-0791 ext. 289
} Fax: 203-776-0795
} Email: yash.agarwal-at-ikonisys.com


From daemon Wed Feb 27 16:11:03 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 27 Feb 2002 16:48:08 -0500
Subject: Microprobe tracks

Contents Retrieved from Microscopy Listserver Archives
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Two questions, 1) How did he put the carbon on? If with a dirty system, he could be getting contamination. 2) What is his sample made of. An electron microprobe uses a large current and can damage some samples. Glass is a prime example.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Wednesday, February 27, 2002 12:59 PM
To: Microscopy-at-sparc5.microscopy.com


Hi:

Sorry if this is a little off our topic line.

I recently carbon coated some samples for a user who took them to a
microprobe for analysis.

He called to ask about the carbon coating's affect on the appearance of
the sample after analysis.

On a previous run, with a different instrument, he said the beam 'toasted'
the area of analysis and he liked that since it showed exactly where the
beam had been. On the instrument he used here, there was no toasting, so no
visible record of the analytical location.

I don't run the probe, I just do the carbon coating. But I thought someone
out there in microscope land might know what I can tell him. He says the
toasting probe was newer and better than the older one we have here. He
said the beam conditions were similar for the two instruments.

I am curious. What are the toast tracks and does the carbon coating have
anything to do with them?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Wed Feb 27 18:35:36 2002



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Wed, 27 Feb 2002 17:26:10 -0700
Subject: RE: Microprobe tracks

Contents Retrieved from Microscopy Listserver Archives
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Jonathan,

When we carbon coat samples, we too get those "toasted" areas that's believed to be due to carbon build up where the
beam interacts with the specimen. We generally use 20KV with a beam current of 20nA. They can be helpful to see
exactly where the analysis was done and the beam diameter as well. However, when the sample is mounted in conductive
mounting material and no carbon coating is required, the "toasted" areas aren't nearly as visible if at all at the same
operating parameters.


Harry Ekstrom
Materials Laboratory

*Phone: (602) 231-2744
?Fax: (602) 231-1547
*e-mail: harry.ekstrom-at-honeywell.com
{mailto:harry.ekstrom-at-honeywell.com}




From daemon Wed Feb 27 19:05:49 2002



From: Diane G. Miller :      millerd-at-coho.net
Date: Wed, 27 Feb 2002 18:57:11 -0600
Subject: SIMS

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

I need some information. I hope I will get some responses from you.
I was wondering what the difference is between a SIMS Secondary Ion
Mass Spectrometer and an Ion Beam Electron Microscope. Please excuse
my ignorance, but I've tried looking on the web, and I haven't found
the explanation that I need.

Any help would be appreciated.

Thank you in Advance.

Diane

{mailto:millerd-at-coho.net} millerd-at-coho.net



From daemon Wed Feb 27 21:50:09 2002



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Wed, 27 Feb 2002 19:32:58 -0800
Subject: Re: Microprobe tracks

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopy list:

The toasting process is probably similar to the mechanism that causes
contamination scan marks. The electron beam interacts with the carbon
molecules and ionizes them. The react with each other to form amorphous
polymers that are a visible to the secondary electron detectors. For
uncoated specimens the "toasting" marks are just plain hydrocarbon
contamination deposits. The beam is interacting with the residual
hydrocarbon partial pressure to create ions that follow the e beam down to
the surface to form that polymer goo.

Ronald Vane
XEI Scientific
anticontamination systems
www.SEMCLEAN.com
650-369-0133




From daemon Wed Feb 27 23:29:29 2002



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Thu, 28 Feb 2002 16:22:03 +1100
Subject: Re: TRANSWELL MEMBRANES

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Hi John,

It's also difficult with EM sections. I've always assumed the
wrinkling is due to the resin expanding and the membrane not. I
haven't tried this, but dissolving the resin with ethoxide would
probably fix the problem.

Diana

At 10:15 AM -0500 27/2/02, "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com wrote:
}
} has anyone out there had any experience in cutting one micron
} sections of transwell membranes? a tech in my lab is having trouble
} getting them flat on a glass slide. he has wrinkles which is
} unacceptable for publication. i have never worked with them. any
} advice would be great.
} john hoffpauir

--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Thu Feb 28 01:29:36 2002



From: Leon Smuts :      PLBLS-at-puknet.puk.ac.za
Date: Thu, 28 Feb 2002 09:17:07 +0200
Subject: Re: Microprobe tracks

Contents Retrieved from Microscopy Listserver Archives
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Dear Jonathan,

The "toasted" appearance on the surface of the sample at the area/point of analysis is extra carbon that is deposited from the probe's inside vacuum environment. The source of this carbon is from the vacuumpumps, especially the rotary pump.

The longer the area is exposed to the electron beam, the higher the beam current, the more carbon is deposited on that spot or raster area.

These extra carbon deposited on your beam on the spot of analysis causes the matrix correction prosedures and standarizing procedures to be incorrect as the standards and unknown samples are normally carbon coated to a very precise thickness of 25 nanometers.

Best wishes, Leon

Skool vir Omgewingswetenskappe en Ontwikkeling: Geologie, GIS
\\\\\\\\\\\\\\\\} URL: http://www.puk.ac.za } WWW} \\\\\\\\\\\\\\\\\\\
Leon Smuts-Electronmicroprobe-Potchefstroom University-South Africa
/////////////} mailto: plbls-at-puknet.puk.ac.za } eMail} ///////////////


} } } Jon Krupp {jmkrupp-at-cats.ucsc.edu} 02/27/02 07:59PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi:

Sorry if this is a little off our topic line.

I recently carbon coated some samples for a user who took them to a
microprobe for analysis.

He called to ask about the carbon coating's affect on the appearance of
the sample after analysis.

On a previous run, with a different instrument, he said the beam 'toasted'
the area of analysis and he liked that since it showed exactly where the
beam had been. On the instrument he used here, there was no toasting, so no
visible record of the analytical location.

I don't run the probe, I just do the carbon coating. But I thought someone
out there in microscope land might know what I can tell him. He says the
toasting probe was newer and better than the older one we have here. He
said the beam conditions were similar for the two instruments.

I am curious. What are the toast tracks and does the carbon coating have
anything to do with them?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu






From daemon Thu Feb 28 02:08:40 2002



From: Legendre Olivier :      Legendre-at-exchange.brgm.fr
Date: Thu, 28 Feb 2002 09:02:07 +0100
Subject: JEOL5400LV

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I have been in charge recently of our SEM lab, in which there is a rather
idle JEOL5400LV (with a Kevex EDS) microscope. According to the person who
is in charge of running this machine, the capabilities of this SEM are
restricted to study uncoated dry samples, preferably mineral.
Does anybody have further experience on this machine (or an upgraded one?)
like imaging wet samples, etc.?

Thank you for helping

Olivier Legendre
BRGM
3, avenue C. Guillemin
45060 ORLEANS CEDEX 2 FRANCE
Tel: (33) 0 238 64 38 03
Fax: (33) 0 238 64 37 11
e-mail: o.legendre-at-brgm.fr






From daemon Thu Feb 28 03:23:10 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Thu, 28 Feb 2002 10:14:22 +0100
Subject: Re: Live Cell Imaging: Stage Drift and Shutter Woes

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I have some suggestions for the second problem you mention. I have used
our own in-house developed multi-position time-lapse system for years
and I have experienced the same problems. We used a system with an
incubation chambre in which we could keep cells on 37 degrees centigrade
or on room temperature (18 - 21 degrees centigrade). We also noticed a
small drift which was specially annoying on high N.A. lenses as you too
have noticed.

After some experiments we foud out that is was caused by the response of
the metal of the microscope to changes in the equipment temperature. We
often did 10 time-lapse movies in parallel on one micrscope over the
weekend and we could monitor that the changes in the room temperature
had an influence on the position of the motorised stage (X,Y and Z
-axis). After we found out what caused the problem we simply refocused
for each position at each time-lapse cycle, which solved the problem for
us.

Another option could be to put the entire microscope in a temperature
controlled environment. In most temperature control system, only part of
the microscope is inside the incubation chamber, all the rest is exposed
to the changes of the room temperature. Even in a modern building, the
air conditioning system is not precise enough to stabilise the
temerature enough for high-resolution microscopy.

Best regards,

Peter
--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

================================
} Hello all,
} We are attempting to perform time-lapse imaging of GFP-labelled cells on a Zeiss Axiophot 1 (an upright microscope). There are two aspects of the system that cause us trouble. The first is our Uniblitz shutter, which is driven via parallel port by a module that is part of the Universal Imaging Metamorph v.4.0 suite. The shutter responds to oscilloscope-generated current pulses to provide open (exposure) times of 20 ms (as stated in the specs). We require open times of 100-200 ms, but the shortest pulse interval that can be generated through the parallel cable by any exposure control function in the software is 500- 600 ms. No-one connected with U.I. or the company that sold us our system can explain why this is the case, and how we might achieve shorter exposure times. Is there anyone out there with experience or knowledge that might be brought to bear on this issue?
} The second problem is more troubling. Even without a heating chamber, we experience a small, variable, but problematic downward drift of the specimen stage. The total drift is ~0.5 -1um per minute (sometimes more or less), but is enough to scuttle velocity measurements with our 1.4 NA lens. To solve this, we bought a Prior Optiscan z-axis focus controller, which meshes a gear with the microscope's coarse focus gear. We thought this might prevent the subtle stage slippage that is compromising our observations. No dice. Again, does anyone have an insight, knowledge or words of wisdom? Thanks in advance.
}
} .


From daemon Thu Feb 28 08:40:01 2002



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Thu, 28 Feb 2002 09:19:10 -0500
Subject: Re: TRANSWELL MEMBRANES

Contents Retrieved from Microscopy Listserver Archives
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} John,

We haven't cut these types of membranes, but when we thick section large windowed plastics they tend to wrinkle on slides. What we do
is float them onto a drop of water on the slide and heat on a hot plate to evaporate the water. Once thoroughly dry, sections are stained,rinsed and immediately coversliped with permount (careful not to move in the xy axis when lightly compressing). This usually prevents section
wrinkles.

Good luck

Mike D.
JHMI Microscope Facility

P.S. slower sectioning and smaller faces may help.


"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} has anyone out there had any experience in cutting one micron sections of transwell membranes? a tech in my lab is having trouble getting them flat on a glass slide. he has wrinkles which is unacceptable for publication. i have never worked with them. any advice would be great.
} john hoffpauir



From daemon Thu Feb 28 09:06:22 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 28 Feb 2002 09:58:29 -0500
Subject: Re: TRANSWELL MEMBRANES

Contents Retrieved from Microscopy Listserver Archives
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}
} has anyone out there had any experience in cutting one micron
} sections of transwell membranes? a tech in my lab is having trouble
} getting them flat on a glass slide. he has wrinkles which is
} unacceptable for publication. i have never worked with them. any
} advice would be great.
} john hoffpauir
***************************
I work wit them a lot....they always wrinkle. I have tried vapors,
heat, drying them down slowly on a large water drop,drying them down
quickly on a small water drop...you get the picture. I can offer no
solution for your tech, just sympathy. My clients usually opt for
taking a high mag shot of a relatively flat area.
Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Feb 28 09:51:04 2002



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Thu, 28 Feb 2002 10:43:36 -0500
Subject: Polarized light Microscopy Workshop, New york Microscopic Society - LM

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New York Microscopical Society

30 North Mountain Avenue

Montclair, NJ 07042



Bernard Friedman

Memorial Workshop





Polarized Light Microscopy

April 20,27, May 4 & 11, 2002


An advanced course on polarized light microscopy which will cover the
following topics:

The nature of polarized light

The origin and interpretation of interference colors

Birefringence and crystal orientation

The Indicatrix

Compensation and variable compensators

Interference figures and their interpretation


The workshop will consist of four Consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of polarized
light microscopy. The course instructors include Jan Hinsch of Leica, Inc.,
Mary McCann of McCann Imaging, John Reffner of SensIR and N.Y.M.S.
Instructor Don O'Leary.

WHEN: April 20, 27, May 4,&11, 2002, from 10 A.M. to 4 P.M.

WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043

COST: $330 for N.Y.M.S. members, $360 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: advanced course for those who have completed "The Use of the
Microscope" or are experienced in microscopy and familiar with the theory of
its use.

HOW: Register using the form below. Limited to the first 12 registrants.

Return form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 e-mail
donoleary-at-att.net

PLEASE POST

----------------------------------------------------------------------------
-------------------------------------

Registration Form

Polarized Light Microscopy

N.Y.M.S. Member_________________ ($330) Non-Member__________($360)

Name_____________________________________________________________

Address___________________________________________________________

Phone (W)_________________________(H)______________________________

e-mail________________________________________

Donald O'Leary
Curator & Education Chair
New York Microscopical Society
6 Chittenden Road
Fair Lawn, NJ 07410
(201) 797-8849






From daemon Thu Feb 28 10:31:24 2002



From: Andrew Ochalski :      AOCHALSK-at-science.uottawa.ca
Date: Thu, 28 Feb 2002 11:24:23 -0500
Subject: Live Cell Imaging: Stage Drift and Shutter Woes

Contents Retrieved from Microscopy Listserver Archives
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{color} {param} 0100,0100,0100 {/param} Hello all, {FontFamily} {param} Arial {/param}


{/color} {FontFamily} {param} Times New Roman {/param} I am once again in awe of the marriage of expertise and generosity on this
listserver. Thank you all for your replies. Since yesterday, I have received
many replies and would particularly like to thank Dan Focht of Bioptechs
for a lengthy telephone session. Here's a progress report so far. I
neglected to mention in my original post (sorry!) that, in order to rule out
thermal effects from our heating chamber, we began to run time-lapse
series on microscope slides of fixed cells (no chamber). Since the chamber
is quite heavy, we also eliminated stage drift due to this added weight. The
drift was still present. As many of you have pointed out, the location of
heating/cooling vents, heat-generating equipment and even exothermic grad
students might influence focus. We have reasoned that thermal instability
should shift the focal plane both above and below the specimen plane and
in an almost random fashion. The focal plane always shifts to a point above
the specimen (consistent with the stage lowering). In addition, the rate at
which this occurs is more or less reproducible, regardless of the number of
bodies present or milling about in the room and of the average room
temperature (though we haven't monitored the stage, objective or slide
temperature systematically). So, we have tentatively concluded that the
problem is related to the specific focus mechanism of our microscope. In
conjunction with our local Zeiss service rep, we will be attempting a
solution based on this conclusion. I won't describe it at this time since it
may only be applicable to a small number of microscopes and probably
shouldn't be undertaken by people (like me) without a detailed
understanding of the focusing mechanism of the microscope in question.
Our service rep has reluctantly agreed to try a simple alteration of the
mechanism. I'll let you know if it works and what it was, if it does. As to
the shutter open time... Many of you have suggested plausible causes for
the problem. I have tried many of the "common-sense" solutions suggested
(e.g., removing unnecessary peripherals, resident programs and tweaking
settings in MetaMorph's Device and Drop-in Manager systems). Other
aspects of system performance have improved. More than one of you has
suggested construction of a one shot, leading edge-triggered TTL pulse
generator. This seems to me to be the best way to get the shutter to do
what it's told. I'll keep you posted on this as well. Thank you all
again.


{nofill}


From daemon Thu Feb 28 10:34:14 2002



From: Victoria Madden :      vmadden-at-apex.med.unc.edu
Date: Thu, 28 Feb 2002 11:32:02 -0500
Subject: Re: Transwell membranes

Contents Retrieved from Microscopy Listserver Archives
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I have found the best way to cut Transwell and other membranes is to
orient the membrane parallel to the knife edge (horizontal) instead
of the conventional vertical orientation. For 1 micrometer and 70nm
sections, use a slow cutting speed (0.5mm/s or less) and you should
be able to produce chatter-free sections. The investigators that I
work with usually want to see migration of the cells through the
membrane pores plus the cell layers on both sides of the membrane,
and I find cutting in this manner produces better results (not to say
that I don't get a wrinkle or two now and then). For thick sections,
the old fashioned gelatin-chrome alum coated slides work better than
poly-lysine or Plus slides--the sections adhere well and usually
remain wrinkle-free after staining with toluidine blue. I also embed
the membranes in the harder formulation of Spurr's resin and highly
recommend using a diamond knife for both thick and ultrathin
sectioning.
--
Victoria J. Madden
Microscopy Services Laboratory
Pathology and Laboratory Medicine
University of North Carolina at Chapel Hill

vmadden-at-med.unc.edu




From daemon Thu Feb 28 10:38:07 2002



From: tbargar-at-unmc.edu
Date: Thu, 28 Feb 2002 10:31:29 -0600
Subject: Need Info. about Berylium and Berylium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
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An individual new to EM wanted to learn from me how to formvar-carbon coat
slotted grids. The person bought grids made of a Berylium-Copper alloy. I
called the vendor and was warned about the danger of Berylium and it's
vapor. I told the invidual to get copper grids for our use. Now in all my
years in EM I have not worked with Berylium. Would someone out there send
me some information on the hazards of Berylium? Also what applications are
Berylium-Copper or Berylium only grids used for? Any information is
appreciated, thanks.

Tom Bargar
EM Lab, UNMC
tbargar-at-unmc.edu
402-559-7347



From daemon Thu Feb 28 11:01:45 2002



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Thu, 28 Feb 2002 08:54:19 -0800
Subject: RE: JEOL5400LV

Contents Retrieved from Microscopy Listserver Archives
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Hello Oliver,
We have a JEOL 5900LV in our lab, which I think is a minor
update/upgrade to the machine you have. The "LV" designation typically
means that the microscope is capable of "low vacuum" operation. In our
case, we didn't purchase that option, even though we have that model number
designation on our scope. You will probably need to check your
documentation to verify if you have that feature, and how to use it. You
might also want to check with the local JEOL service/sales people who sold
you the instrument to determine its capabilities (http://www.jeol.com).
We use ours quite heavily for typical high vacuum SEM purposes, and
it performs well for a standard tungsten filament SEM. Most of our work has
been with glasses, ceramics, metals, and polymers. When you say "wet
imaging", that typically refers to an evironmental SEM, which is a more
sophisticated type of SEM (lenses, detectors, and vacuum pumping system
modified to accomodate chamber conditions that are different than near the
gun.). Good luck, and enjoy!

-Brad



----------
From: Legendre Olivier
Sent: Thursday, February 28, 2002 12:02 AM
To: 'Microscopy-at-MSA.Microscopy.Com'
Subject: JEOL5400LV


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Hi all,

I have been in charge recently of our SEM lab, in which there is a
rather
idle JEOL5400LV (with a Kevex EDS) microscope. According to the
person who
is in charge of running this machine, the capabilities of this SEM
are
restricted to study uncoated dry samples, preferably mineral.
Does anybody have further experience on this machine (or an upgraded
one?)
like imaging wet samples, etc.?

Thank you for helping

Olivier Legendre
BRGM
3, avenue C. Guillemin
45060 ORLEANS CEDEX 2 FRANCE
Tel: (33) 0 238 64 38 03
Fax: (33) 0 238 64 37 11
e-mail: o.legendre-at-brgm.fr








From daemon Thu Feb 28 11:08:05 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 28 Feb 2002 09:03:52 -0800
Subject: Re: Microprobe tracks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jonathan,
The marks that a microprobe can make on a sample surface can come from many
sources, but a clean, evaporated carbon coat should not be one of them. The
microprobe itself can lay down contamination from its own vacuum system, the
sample can evolve contamination that is carbonized in the high-current beam
or other sources of contamination might be present. One of the sources of
contamination I found when I did a study of contamination rates and
contamination clean-off by air-jet, was that an incidence of the beam
hitting the mounting epoxy at the edge of a sample caused increased
contamination in the instrument for three days. If you scan or test at the
edge of a mounted specimen you can see the marks of the scan in the light
microscope, where the epoxy has been boiled away by the beam.
I would say that the absence of the toast marks in your microprobe was a
good thing and indicates your vacuum system is better than the other one he
used, even if he likes these marks.
At 09:59 AM 2/27/02 -0800, you wrote:
} Hi:
}
} Sorry if this is a little off our topic line.
}
} I recently carbon coated some samples for a user who took them to a
} microprobe for analysis.
}
} He called to ask about the carbon coating's affect on the appearance of
} the sample after analysis.
}
} On a previous run, with a different instrument, he said the beam 'toasted'
} the area of analysis and he liked that since it showed exactly where the
} beam had been. On the instrument he used here, there was no toasting, so no
} visible record of the analytical location.
}
} I don't run the probe, I just do the carbon coating. But I thought someone
} out there in microscope land might know what I can tell him. He says the
} toasting probe was newer and better than the older one we have here. He
} said the beam conditions were similar for the two instruments.
}
} I am curious. What are the toast tracks and does the carbon coating have
} anything to do with them?
}
} Thanks
}
} Jonathan Krupp

Regars,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Thu Feb 28 11:08:23 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Thu, 28 Feb 2002 12:02:49 -0500
Subject: Re: JEOL5400LV

Contents Retrieved from Microscopy Listserver Archives
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This is a beautiful low (lousy) vacuum SEM. Its sounds like the "restricted
to study uncoated dry samples, preferably mineral" was put on the
microscope by someone at the SEM Lab who didn't want any other
samples/users using the scope.

I've often seen where the "Materials People" and the Geology people fobid
messy unstable biological samples in their EM's in order to keep the scopes
clean (particularly when dealing with analytical systems). (Now, where do the
clays and rubber people fit in??)

Now, before you get someone upset you might find out the history of the
"restricted to study uncoated dry samples, preferably mineral" and who paid
for the scope (got the grant). But an EM is a terrible thing to waste.

On 28 Feb 2002, at 9:02, Legendre Olivier wrote:

} Hi all,
}
} I have been in charge recently of our SEM lab, in which there is a rather
} idle JEOL5400LV (with a Kevex EDS) microscope. According to the person who
} is in charge of running this machine, the capabilities of this SEM are
} restricted to study uncoated dry samples, preferably mineral.
} Does anybody have further experience on this machine (or an upgraded one?)
} like imaging wet samples, etc.?
}
} Thank you for helping
}
} Olivier Legendre
} BRGM
} 3, avenue C. Guillemin
} 45060 ORLEANS CEDEX 2 FRANCE
} Tel: (33) 0 238 64 38 03
} Fax: (33) 0 238 64 37 11
} e-mail: o.legendre-at-brgm.fr
}
}
}
}
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Thu Feb 28 11:56:15 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 28 Feb 2002 11:49:03 -0600
Subject: Anyone have Sesame WDS?

Contents Retrieved from Microscopy Listserver Archives
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Greetings all,

I would be interested to hear from anyone who is still using a Kevex
Sesame24/Microspec 2A WDS system. If you have one that is retired, I would
especially have an interest in the possibility of obtaining spare cards.
Mine is working but have no spares...

Another question about the Sesame... I no longer have the Kevex 8000/Delta
EDS to which the WDS sent data for quantitation. Has anyone mated the
Sesame to a different EDS/software package to do quantitation?

Thanks, Woody
--------------
Woody White
McDermott Technology, inc
nwwhite-at-mcdermott.com


From daemon Thu Feb 28 12:00:51 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 28 Feb 2002 11:31:50 -0600
Subject: Re: JEOL5400LV

Contents Retrieved from Microscopy Listserver Archives
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We have a Hitachi LV here. We go back and forth between high vacuum mode
and low vacuum quite a bit. We have a JEOL 840A down the hall and try to
steer people there if they really don't need the low vacuum mode. It is
simply a matter of balancing the use.

I am not familiar with the 5400 capabilities. If it is like ours, it is not
a true environmental scope. We can only manage an atmosphere of 2 Torr (270
Pa) which is not enough to keep wet samples wet for very long. Also, the
detectors and such are not optimized for true environmental mode. But it
does great for insulating specimens.

At 09:02 AM 2/28/02 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Feb 28 15:15:50 2002



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 28 Feb 2002 16:06:12 -0500
Subject: Re: Need Info. about Berylium and Berylium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
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This is not a "reply" to the post, but perhaps an extension of the question.

Why would Cu-Be grids be used? Cu-Be is an interesting and quite
widely-used class of alloys (see http://microstructure.copper.org), but I
don't see why it would be used for EM grids - unless, that is, the grids we
loosely call "copper" are in fact all made of Cu-Be?

On the subject of the posting, I can't imagine that Cu-Be would be as
widely used as it is if it were as toxic as Be-metal. I'm sure the
alloying must drastically reduce the hazard, but this is not my area of
expertise.

Tony


At 10:31 AM 2/28/2002 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*




From daemon Thu Feb 28 15:54:04 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Thu, 28 Feb 2002 15:39:59 -0600
Subject: RE: cryostats

Contents Retrieved from Microscopy Listserver Archives
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We have two Microm HM505 cryostats: one HM505N (non-motorized advance) and
one HM505E (motorized advance). We've not had too much trouble with them,
considering we've had one for at least five years and the other, which was
recently donated to us, is probably older. The few problems we've
experienced with the one we purchased have been outside the norm and were
resolved relatively quickly (this doesn't take into consideration operator
error -- an inexperienced user ran the specimen chuck into the knife holder
and put a large dent in it).

Both units section well. In fact, the batch of blocks I cut on the HM505E
earlier this week were probably the best I've ever cut (and we still have an
old IEC workhorse around here somewhere).

I believe Microm is carried by Richard-Allan Scientific and distributed by
Fisher.

Hope this helps you out,

Jaclynn M. Lett jlett-at-cid.wustl.edu

Staff Manager, EM Core Facility
Fay and Carl Simons Center of Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110


From daemon Thu Feb 28 17:24:46 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 28 Feb 2002 15:20:58 -0800
Subject: Re: SIMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Did not see any response yet so I'll give it a try.

A good reference for SIMS is Wilson, R., Stevie, F.
and Magee, C. (1989). Secondary ion mass spectrometry.
New York: John wiley & Sons. ISBN 0-471-51945-6

Ion beam microscopy is a mode which is available in
some, if not all (not sure) SIMS units. The distinction
is made between depth profiles, side wall ion contributions
and other effects. Large area ratios are typically
required for probe mode to exclude secondary ions
from the sidewalls when the beam is in the center
of the crater. Alternatively, secondary ions are
rejected outside the center of the crater "with an
aperture for the ion microscope mode" (p. 1.5-1).

I haven't seen much other SIMS reference material either.
Maybe it is a secret cult?

gary g.


At 04:57 PM 2/27/2002, you wrote:

} Hello All,
}
} I need some information. I hope I will get some responses from you. I was
} wondering what the difference is between a SIMS Secondary Ion Mass
} Spectrometer and an Ion Beam Electron Microscope. Please excuse my
} ignorance, but I've tried looking on the web, and I haven't found the
} explanation that I need.
}
} Any help would be appreciated.
}
} Thank you in Advance.
}
} Diane
}
} {mailto:millerd-at-coho.net} millerd-at-coho.net
}



From daemon Thu Feb 28 18:08:24 2002



From: =?iso-8859-1?Q?G=F6ran?= Axelsson :      axelsson-at-acc.umu.se
Date: Fri, 01 Mar 2002 00:08:49 +0100
Subject: SEM books on mineral analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello!

I'm looking for a book or some other resources on mineral analysis with EDS.

I have read a lot of information on the net about SEM / TEM and other
instruments so I have a good theoretical knowledge about the process
behind EDS but lacks the practical bit.

My background is a MS in physics, some chemistry and some geology.
I have had some mineral samples analysed in a Russian lab and now I
want to learn more about the practical side.
How to interprete the results, how to prepare specimens, which problems
could occur....

Is there a book or some other information source that you could recommend
for me?

I will try to visit a lab for some hands on experience during the spring, but I
would like to be well prepared so I could get the most out of the visit.

My final goal is to find a cheap used SEM with EDS to set up a small lab for
mineral analyses.

Regards, Göran Axelsson, Sweden




From daemon Thu Feb 28 22:52:16 2002



From: Tony Bruton :      Bruton-at-nu.ac.za
Date: Fri, 01 Mar 2002 11:13:45 +0200
Subject: Re: JEOL5400LV

Contents Retrieved from Microscopy Listserver Archives
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Hello Olivier:

JEOL5400LV probably is a first generation LV SEM. The weak point is its
needle control valve on LV mode. It needs pay much attention to monitor
the dial reading of pressure and manually adjust the pressure and make it
stable. The new version LV SEMs have no manual control valve on the panel.

The LV SEM, with EDS attached on is very useful combination. You can do a
lot of work on this system: EDS analysis on HV or LV, coated (on HV) or
uncoated (on LV) samples, and LOW kv imaging (try 0.5-3.0 KV on HV mode)
without coating. It even does not need to wear gloves at all. Get rid of
restricted limitation and enjoy this powerful tool.

Zhiyu Wang, Sr. Engineer
Maxtor Corp.
Milpitas, CA USA


----- Original Message -----
} From: "Legendre Olivier" {Legendre-at-exchange.brgm.fr}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, February 28, 2002 8:02 AM


My comment to add to the discussion having examined both LV and ESEM
microscopes prior to purchasing an ESEM.

The strength of the LV SEM's is their ability to view SEM specimens
without the need for coating. This they do very well and it is an
enormously useful feature. Their ability to view wet specimens is
limited by the fact that you cannot hold a particular RH (relative
humidity) state for any length of time - as you can in the ESEM. In the
LVSEM the water from a wet specimen would tend to leave the specimen
rapidly. That said, we often use our ESEM in the 2 torr range for the
examination of damp and/or greasy uncoated specimens where the
maintanance of exact, or higher, water saturation levels is not
critical.


Tony Bruton
University of Natal
South Africa

} } } "Richard Edelmann" {edelmare-at-muohio.edu} 02/28/02 07:02PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America


This is a beautiful low (lousy) vacuum SEM. Its sounds like the
"restricted
to study uncoated dry samples, preferably mineral" was put on the
microscope by someone at the SEM Lab who didn't want any other
samples/users using the scope.

I've often seen where the "Materials People" and the Geology
people fobid
messy unstable biological samples in their EM's in order to keep the
scopes
clean (particularly when dealing with analytical systems). (Now, where
do the
clays and rubber people fit in??)

Now, before you get someone upset you might find out the history
of the
"restricted to study uncoated dry samples, preferably mineral" and who
paid
for the scope (got the grant). But an EM is a terrible thing to
waste.

On 28 Feb 2002, at 9:02, Legendre Olivier wrote:

} Hi all,
}
} I have been in charge recently of our SEM lab, in which there is a
rather
} idle JEOL5400LV (with a Kevex EDS) microscope. According to the
person who
} is in charge of running this machine, the capabilities of this SEM
are
} restricted to study uncoated dry samples, preferably mineral.
} Does anybody have further experience on this machine (or an upgraded
one?)
} like imaging wet samples, etc.?
}
} Thank you for helping
}
} Olivier Legendre
} BRGM
} 3, avenue C. Guillemin
} 45060 ORLEANS CEDEX 2 FRANCE
} Tel: (33) 0 238 64 38 03
} Fax: (33) 0 238 64 37 11
} e-mail: o.legendre-at-brgm.fr
}
}
}
}
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."




From daemon Fri Mar 1 03:59:08 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 01 Mar 2002 06:37:47 -0500
Subject: Re: Need Info. about Berylium and Berylium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
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Hello,
We met also the problem with the continuous one direction de-focusing with a
heavy 16-slides Maerzhaeuser stage.
We solved it in the software. Simply the drift was time calibrated. The time
lapse experiment is running while the stage Z-drive
compensates the drift according to the calibration. We are able to adapt
this procedure almost to any motorised microscope.

Best regards

Josef Mikes

Laboratory Imaging, s.r.o.
Nad Upadem 901/63
CZ-149 00 Prague 4
Tel: (+420-2) 6791 4552
Fax: (+420-2) 7173 2657
e-mail: mailto:josef.mikes-at-lim.cz
web site: www.laboratory-imaging.com


-----Original Message-----
} From: Andrew Ochalski [mailto:AOCHALSK-at-science.uottawa.ca]
Sent: Thursday, February 28, 2002 5:24 PM
To: Microscopy-at-sparc5.microscopy.com


Tony,
I can't answer why it would be used, except that Cu-Be is much harder
than Cu. The main danger from Cu-Be is if you grind it or burn it, but
the grids are so small that grinding is fairly unlikely. As used in
non-magnetic tools, etc., you're right. It's very safe.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Tony Garratt-Reed wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This is not a "reply" to the post, but perhaps an extension of the
} question.
}
} Why would Cu-Be grids be used? Cu-Be is an interesting and quite
} widely-used class of alloys (see http://microstructure.copper.org),
} but I don't see why it would be used for EM grids - unless, that is,
} the grids we loosely call "copper" are in fact all made of Cu-Be?
}
} On the subject of the posting, I can't imagine that Cu-Be would be as
} widely used as it is if it were as toxic as Be-metal. I'm sure the
} alloying must drastically reduce the hazard, but this is not my area
} of expertise.
}
} Tony
}
}
} At 10:31 AM 2/28/2002 -0600, you wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } An individual new to EM wanted to learn from me how to formvar-carbon
} } coat
} } slotted grids. The person bought grids made of a Berylium-Copper
} } alloy. I
} } called the vendor and was warned about the danger of Berylium and it's
} } vapor. I told the invidual to get copper grids for our use. Now in
} } all my
} } years in EM I have not worked with Berylium. Would someone out there
} } send
} } me some information on the hazards of Berylium? Also what
} } applications are
} } Berylium-Copper or Berylium only grids used for? Any information is
} } appreciated, thanks.
} }
} } Tom Bargar
} } EM Lab, UNMC
} } tbargar-at-unmc.edu
} } 402-559-7347
}
}
}
} * * * * * * * * * * * * * * * * * * * * * * * * * *
} * Anthony J. Garratt-Reed M.A., D.Phil.
} * MIT, Room 13-1027
} * 77 Massachusetts Avenue
} * Cambridge, MA 02139-4307
} * USA
} * Phone: (617) 253-4622
} * Fax: (617) 258-6478
} *
}
}
}
}




From daemon Fri Mar 1 07:14:00 2002



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Fri, 1 Mar 2002 08:05:54 -0500
Subject: RE: SIMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You are correct in your 'secret cult' image, Gary. SIMS is largely a
well-kept secret of both the geoscience and semiconductor communities, with
the vast majority of materials scientists being unaware of its capabilities
in trace element detection (including even hydrogen), sputter depth
profiling, elemental imaging, isotope ratio age-dating and so forth. We
here in Ottawa at a federal materials science-oriented lab have had a SIMS
for nearly 15 years as a complement to our SEM, TEM and electron microprobe.
Like our XPS and Auger, however, the SIMS is not used nearly as much as the
EMs, but clients in the above two materials communities use it regularly.
Let me note that the 'new kid on the block' in the SIMS community is
something called a nanoSIMS, with a 50 nm beam (as opposed to the micron-ish
beam of a regular SIMS) and simultaneous detection of up to 5 elements (much
like a microprobe with WDX detectors). To date there are only two in North
America, no surprise given the ~$2M+ US price tag.

However, I believe that Diane was referring to the differences between a
SIMS and a focused ion beam (FIB) system, which is essentially a scanning
ion microscope. In a FIB a much higher energy ion beam (30-50 keV as
opposed to only several keV for a SIMS) is focused by electrostatic lens
down to as little as 5 nm and scanned as in an SEM. The ion beam generates
both secondary ions and secondary electrons, which can be captured to form
the corresponding two types of images. Generally speaking, the resolution
is intermediate between an LaB6 emitter SEM and a field emission (FE) SEM.
The use of an electron flood gun permits good imaging of insulating
materials. The most important difference between a SIMS and a FIB is that,
while the former uses the scanned beam to sputter a crater for depth
profiling, the latter uses its more energetic beam to accomplish in-situ
'micromachining'. Thus FIBs had their inception in the microelectronics
community to conduct fine-scale repairs on devices. More recently, they
have impacted seriously on general materials science via use of this
micromachining capability to prepare parallel-sided thin sections for TEM in
various ways. Finally, alas, FIBs have no analytical (EDXS) capability to
date, save for a few models that combine both a ion beam column and an
electron beam column (thus called dual beam FIBS), wherein an EDXS detector
can be used in conjunction with the latter.

Attendees of M&M 2002 in Quebec City should check out the FIB session
chaired by Lucille Gianuzzi and Mike Phaneuf. I guarantee you'll be
impressed.

Tom

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada

} ----------
} From: Gary Gaugler
} Sent: Thursday, February 28, 2002 6:20 PM
} To: Diane G. Miller
} Cc: MSA listserver
} Subject: Re: SIMS
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Did not see any response yet so I'll give it a try.
}
} A good reference for SIMS is Wilson, R., Stevie, F.
} and Magee, C. (1989). Secondary ion mass spectrometry.
} New York: John wiley & Sons. ISBN 0-471-51945-6
}
} Ion beam microscopy is a mode which is available in
} some, if not all (not sure) SIMS units. The distinction
} is made between depth profiles, side wall ion contributions
} and other effects. Large area ratios are typically
} required for probe mode to exclude secondary ions
} from the sidewalls when the beam is in the center
} of the crater. Alternatively, secondary ions are
} rejected outside the center of the crater "with an
} aperture for the ion microscope mode" (p. 1.5-1).
}
} I haven't seen much other SIMS reference material either.
} Maybe it is a secret cult?
}
} gary g.
}
}
} At 04:57 PM 2/27/2002, you wrote:
}
} } Hello All,
} }
} } I need some information. I hope I will get some responses from you. I
} was
} } wondering what the difference is between a SIMS Secondary Ion Mass
} } Spectrometer and an Ion Beam Electron Microscope. Please excuse my
} } ignorance, but I've tried looking on the web, and I haven't found the
} } explanation that I need.
} }
} } Any help would be appreciated.
} }
} } Thank you in Advance.
} }
} } Diane
} }
} } {mailto:millerd-at-coho.net} millerd-at-coho.net
} }
}
}


From daemon Fri Mar 1 07:19:10 2002



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Fri, 1 Mar 2002 07:29:44 -0600
Subject: Re: SIMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Thursday, February 28, 2002 9:41 AM
To: NewSub-at-sparc5.microscopy.com


*********************************************
This Email contains Important Information about
the Microscopy Listserver. Please read it all then
SAVE a copy for future reference. - Nestor
****************************************
To: NewSub-at-MSA.Microscopy.Com
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}



As an occasional practitioner of the cult of SIMS, let me pass along an
excellent web resource on the area.

http://www.simsworkshop.org

There is a good series of books on SIMS in the Springer Series in Chemical
Physics, Secondary Ion Mass Spectrometry, ed. by Benninghoven, Colton, and
Simons, but as these are conference proceedings more than strictly a
reference book their value may initially not be as great as the one you
suggested.

Richard Shalvoy
Arch Chemicals
350 Knotter Drive
Cheshire, CT 06410
(203) 271-4394
rbshalvoy-at-archchemicals.com

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Thursday, February 28, 2002 6:21 PM
To: Diane G. Miller
Cc: MSA listserver


Did not see any response yet so I'll give it a try.

A good reference for SIMS is Wilson, R., Stevie, F.
and Magee, C. (1989). Secondary ion mass spectrometry.
New York: John wiley & Sons. ISBN 0-471-51945-6

Ion beam microscopy is a mode which is available in
some, if not all (not sure) SIMS units. The distinction
is made between depth profiles, side wall ion contributions
and other effects. Large area ratios are typically
required for probe mode to exclude secondary ions
from the sidewalls when the beam is in the center
of the crater. Alternatively, secondary ions are
rejected outside the center of the crater "with an
aperture for the ion microscope mode" (p. 1.5-1).

I haven't seen much other SIMS reference material either.
Maybe it is a secret cult?

gary g.


At 04:57 PM 2/27/2002, you wrote:

} Hello All,
}
} I need some information. I hope I will get some responses from you. I was
} wondering what the difference is between a SIMS Secondary Ion Mass
} Spectrometer and an Ion Beam Electron Microscope. Please excuse my
} ignorance, but I've tried looking on the web, and I haven't found the
} explanation that I need.
}
} Any help would be appreciated.
}
} Thank you in Advance.
}
} Diane
}
} {mailto:millerd-at-coho.net} millerd-at-coho.net
}



From daemon Fri Mar 1 07:48:52 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 1 Mar 2002 08:39:57 -0500
Subject: RE: SEM books on mineral analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Goren (sorry for the missing um- [I'm just a] -lout?),

Now, I'm going to take you at your word and venture into an area
which is NOT really mine yet.
Briefly, I would suggest that you consult with Oxford Instruments
for information on on a product they call "INCA Crystal" (no stock, no
family and other companies such as Thermo NORAN also have such systems in
the $50-$100k price range). These systems can be linked to the ICDD
database for mineral identification and permit collection of diffracted
X-rays phases within the specimen to determine both phase identification and
crystal orientation as well as elemental mapping from EDS.
I hope a practitioner responds, but here is the email of a vendor
rep: Glenn Kinnear (Oxford): kinnear-at-ma.oxinst.com.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu

} ----------
} From: Göran Axelsson
} Sent: Thursday, February 28, 2002 6:08 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: SEM books on mineral analysis
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello!
}
} I'm looking for a book or some other resources on mineral analysis with
} EDS.
}
} I have read a lot of information on the net about SEM / TEM and other
} instruments so I have a good theoretical knowledge about the process
} behind EDS but lacks the practical bit.
}
} My background is a MS in physics, some chemistry and some geology.
} I have had some mineral samples analysed in a Russian lab and now I
} want to learn more about the practical side.
} How to interprete the results, how to prepare specimens, which problems
} could occur....
}
} Is there a book or some other information source that you could recommend
} for me?
}
} I will try to visit a lab for some hands on experience during the spring,
} but I
} would like to be well prepared so I could get the most out of the visit.
}
} My final goal is to find a cheap used SEM with EDS to set up a small lab
} for
} mineral analyses.
}
} Regards, Göran Axelsson, Sweden
}
}
}
}


From daemon Fri Mar 1 07:54:51 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 1 Mar 2002 08:45:09 -0500
Subject: RE: Need Info. about Beryllium and Beryllium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Tom,

62 glorious pages that advertise the dangers of Be from the DOE, probably
its largest user!

URL:http://tis-nt.eh.doe.gov/be/berule.pdf (WARNING! You should have the
Adobe Acrobat Reader before trying this URL)


Hope this helps. You can also try the ToxNet on National Library of
Medicine gateway to MEDLINE: http://gateway.nlm.nih.gov/gw/Cmd.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


} ----------
} From: "tbargar-at-unmc.edu"-at-sparc5.microscopy.com
} Sent: Thursday, February 28, 2002 11:31 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Need Info. about Berylium and Berylium-Copper slotted grids
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} An individual new to EM wanted to learn from me how to formvar-carbon coat
} slotted grids. The person bought grids made of a Berylium-Copper alloy.
} I
} called the vendor and was warned about the danger of Berylium and it's
} vapor. I told the invidual to get copper grids for our use. Now in all
} my
} years in EM I have not worked with Berylium. Would someone out there send
} me some information on the hazards of Berylium? Also what applications
} are
} Berylium-Copper or Berylium only grids used for? Any information is
} appreciated, thanks.
}
} Tom Bargar
} EM Lab, UNMC
} tbargar-at-unmc.edu
} 402-559-7347
}
}
}


From daemon Fri Mar 1 08:29:14 2002



From: =?iso-8859-1?Q?S=F8rensen_Henning_Sund?= :      Henning.S-at-danfoss.com
Date: Fri, 1 Mar 2002 15:22:20 +0100
Subject: JEOL5400LV

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We have a JEOL JSM5400LV that, as far as I now, was one of the very first of
this model. It is true that it is not a true environmental SEM, if you
define that as a SEM in which you can study wet samples.

However, we have great use for the low vacuum mode of the instrument for
looking at insulating samples without coating. The insulating samples are
f.x. polymers, corrosion products and various dusts and contaminations.

The main limitation compared to an environmental SEM is that we are not able
to use the secondaary electron detector in LV mode. Instead we use the
backscatter detector that does quite a good job of "topographic" imaging as
long as we do not need high magnification (~} 5000x).

Yours sincerely,

Henning Sund Sørensen
Materials- and Process Consultant

Danfoss A/S
Central Service
Technology Centre
Nordborgvej 81, L7-S40
6430 Nordborg
Denmark

Ph. +45 7488 2309
Fax. +45 7488 2670
e-mail henning.s-at-danfoss.com

External URL : {http://www.danfoss.com/}


-----Original Message-----
} From: Legendre Olivier [mailto:Legendre-at-exchange.brgm.fr]
Sent: 28. februar 2002 09:02
To: 'Microscopy-at-MSA.Microscopy.Com'


Hi all,

I have been in charge recently of our SEM lab, in which there is a rather
idle JEOL5400LV (with a Kevex EDS) microscope. According to the person who
is in charge of running this machine, the capabilities of this SEM are
restricted to study uncoated dry samples, preferably mineral.
Does anybody have further experience on this machine (or an upgraded one?)
like imaging wet samples, etc.?

Thank you for helping

Olivier Legendre
BRGM
3, avenue C. Guillemin
45060 ORLEANS CEDEX 2 FRANCE
Tel: (33) 0 238 64 38 03
Fax: (33) 0 238 64 37 11
e-mail: o.legendre-at-brgm.fr






From daemon Fri Mar 1 09:10:56 2002



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Fri, 1 Mar 2002 10:01:58 -0500
Subject: Re: Anyone have Sesame WDS?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


About 8-10 years ago we had upgraded our Kevex Sesame/Microspec
system by replacing the Sesame system with a 386 PC and card/software
we purchased from Microspec. We don't use the system often but when
we need the better energy resolution because of peak overlap in the
XEDS it is the only way to go. We had dealt with Steve Carr (really
streching my memory) at Microspec back then. Microspec was taken
over by Oxford Instruments, I believe. This is a link to the
appropriate location in their web site;
http://www.oxford-instruments.com/ANLPDP177.htm

There are other third party systems available as well;
http://www.advancedmicrobeam.com/micro3wd.htm

I have no financial interest in any of these companies.

At the moment we have no plans to excess our Microspec so sorry no
spare boards. Within the last two years we did have a problem with
the high voltage to the detector and we were able to trace the
problem to a bad capacitor on the high voltage board. Otherwise the
detector has performed well.






}
}
} Greetings all,
}
} I would be interested to hear from anyone who is still using a Kevex
} Sesame24/Microspec 2A WDS system. If you have one that is retired, I would
} especially have an interest in the possibility of obtaining spare cards.
} Mine is working but have no spares...
}
} Another question about the Sesame... I no longer have the Kevex 8000/Delta
} EDS to which the WDS sent data for quantitation. Has anyone mated the
} Sesame to a different EDS/software package to do quantitation?
}
} Thanks, Woody
} --------------
} Woody White
} McDermott Technology, inc
} nwwhite-at-mcdermott.com


--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html


From daemon Fri Mar 1 09:13:14 2002



From: Lou Ross :      RossLM-at-missouri.edu
Date: Fri, 1 Mar 2002 09:09:52 -0600
Subject: Re: SEM books on mineral analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Goran,

The book I have used for years is called "SEM Petrology Atlas" by Joann E.
Welton. It was published in 1984 by the American Association of Petroleum
Geologists (Tulsa, OK 74101) as part of the Methods in Exploration Series.
Not sure if it still available, but the ISBN # is 0-89181-653-4.

It contains images and EDS spectra of a variety of minerals - silicates,
carbonates, phosphates, halides, sulfides, sulfates, and oxides.

Hope this helps,
Lou Ross


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.biotech.missouri.edu/emc


From daemon Fri Mar 1 09:22:44 2002



From: Andrew Werner :      werner-at-rosharon.oilfield.slb.com
Date: Fri, 01 Mar 2002 09:16:42 -0600
Subject: Re: SEM books on mineral analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a book called:

_SEM Petrology Atlas_ by Joann E. Welton, published by The American
Association of Petroleum Geologists, Tulsa, OK 74101 USA; Copyright 1984,
ISBN 0-89181-653-4.

It contains SEM micrographs and EDS spectra of various minerals associated
with oil and gas exploration. I am a metallurgist, not a geologist, and
this book has saved my... day... a number of times.

Regards,
Andrew T. Werner
Chief Metallurgist
Shaped Charge Research - Metallurgy Laboratory
Schlumberger Reservoir Completions Technology Center
14910 Airline Road, Rosharon, TX 77583-1590
Voice (281) 285-5272 Fax (281) 285-5273

"We shoot the hippopotamus
with bullets made of platinum
'cause if we used the leaden ones
his hide would surely flatten 'em"
- Author Unknown


At 12:08 AM 3/1/2002 +0100, Göran Axelsson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 1 09:31:41 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 01 Mar 2002 07:28:51 -0800
Subject: RE: SIMS

Contents Retrieved from Microscopy Listserver Archives
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Could be she was refering to SIMS and FIB. Based on
my experience with FEI FIB (old model 611), it has poor
imaging resolution. Their newer models, like the 830,
are as you say, dual beam--ion and electron. The electron beam
is used for imaging while the ion beam is used for
micro machining, etc. FIBs are great for making
microcircuit cross sections and changing runners
on the planar area of a chip.

Supposedly, the FEI dual beam FIB will accept a
SIMS "detector." So it would do a whole bunch of
good tasks. Maybe there is only one spare port.
Either a SIMS detector or x-ray detector could
be fitted.

gary g.


At 05:05 AM 3/1/2002, you wrote:
} You are correct in your 'secret cult' image, Gary. SIMS is largely a
} well-kept secret of both the geoscience and semiconductor communities, with
} the vast majority of materials scientists being unaware of its capabilities
} in trace element detection (including even hydrogen), sputter depth
} profiling, elemental imaging, isotope ratio age-dating and so forth. We
} here in Ottawa at a federal materials science-oriented lab have had a SIMS
} for nearly 15 years as a complement to our SEM, TEM and electron microprobe.
} Like our XPS and Auger, however, the SIMS is not used nearly as much as the
} EMs, but clients in the above two materials communities use it regularly.
} Let me note that the 'new kid on the block' in the SIMS community is
} something called a nanoSIMS, with a 50 nm beam (as opposed to the micron-ish
} beam of a regular SIMS) and simultaneous detection of up to 5 elements (much
} like a microprobe with WDX detectors). To date there are only two in North
} America, no surprise given the ~$2M+ US price tag.
}
} However, I believe that Diane was referring to the differences between a
} SIMS and a focused ion beam (FIB) system, which is essentially a scanning
} ion microscope. In a FIB a much higher energy ion beam (30-50 keV as
} opposed to only several keV for a SIMS) is focused by electrostatic lens
} down to as little as 5 nm and scanned as in an SEM. The ion beam generates
} both secondary ions and secondary electrons, which can be captured to form
} the corresponding two types of images. Generally speaking, the resolution
} is intermediate between an LaB6 emitter SEM and a field emission (FE) SEM.
} The use of an electron flood gun permits good imaging of insulating
} materials. The most important difference between a SIMS and a FIB is that,
} while the former uses the scanned beam to sputter a crater for depth
} profiling, the latter uses its more energetic beam to accomplish in-situ
} 'micromachining'. Thus FIBs had their inception in the microelectronics
} community to conduct fine-scale repairs on devices. More recently, they
} have impacted seriously on general materials science via use of this
} micromachining capability to prepare parallel-sided thin sections for TEM in
} various ways. Finally, alas, FIBs have no analytical (EDXS) capability to
} date, save for a few models that combine both a ion beam column and an
} electron beam column (thus called dual beam FIBS), wherein an EDXS detector
} can be used in conjunction with the latter.
}
} Attendees of M&M 2002 in Quebec City should check out the FIB session
} chaired by Lucille Gianuzzi and Mike Phaneuf. I guarantee you'll be
} impressed.
}
} Tom
}
} Tom Malis
} Group Leader - Characterization
} Materials Technology Laboratory
} Natural Resources Canada (Govt. of Canada)
} 568 Booth St., Ottawa, Canada
}
} } ----------
} } From: Gary Gaugler
} } Sent: Thursday, February 28, 2002 6:20 PM
} } To: Diane G. Miller
} } Cc: MSA listserver
} } Subject: Re: SIMS
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Did not see any response yet so I'll give it a try.
} }
} } A good reference for SIMS is Wilson, R., Stevie, F.
} } and Magee, C. (1989). Secondary ion mass spectrometry.
} } New York: John wiley & Sons. ISBN 0-471-51945-6
} }
} } Ion beam microscopy is a mode which is available in
} } some, if not all (not sure) SIMS units. The distinction
} } is made between depth profiles, side wall ion contributions
} } and other effects. Large area ratios are typically
} } required for probe mode to exclude secondary ions
} } from the sidewalls when the beam is in the center
} } of the crater. Alternatively, secondary ions are
} } rejected outside the center of the crater "with an
} } aperture for the ion microscope mode" (p. 1.5-1).
} }
} } I haven't seen much other SIMS reference material either.
} } Maybe it is a secret cult?
} }
} } gary g.
} }
} }
} } At 04:57 PM 2/27/2002, you wrote:
} }
} } } Hello All,
} } }
} } } I need some information. I hope I will get some responses from you. I
} } was
} } } wondering what the difference is between a SIMS Secondary Ion Mass
} } } Spectrometer and an Ion Beam Electron Microscope. Please excuse my
} } } ignorance, but I've tried looking on the web, and I haven't found the
} } } explanation that I need.
} } }
} } } Any help would be appreciated.
} } }
} } } Thank you in Advance.
} } }
} } } Diane
} } }
} } } {mailto:millerd-at-coho.net} millerd-at-coho.net
} } }
} }
} }



From daemon Fri Mar 1 10:17:01 2002



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Fri, 01 Mar 2002 10:57:11 -0500
Subject: Bio-Rad Confocal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is anyone interested in purchasing a Bio-Rad MRC 600 confocal
microscope system?
Included are:
1. Krypton-Argon Laser (low hours) and power source exciting at 488
568 and 655 nm.
2. Nikon optiphot upright epifluorescence microscope with 10 20 and
60x Plan Apo objective lens', mercury arc lamp and power source.
3. Fine focus control stepping motor.
4. Compaq Desktop Pro pentium pc platform wtih Comos and Som
software, and two color monitors.
5. Mitsubishi Dye-sublimation printer.

This system is operational and available for $10,000.000. We will ship,
set-up and train the buyer. Please contact Michael Delannoy
at (410) 955-1365 or via e-mail.

Thank you,
Michael Delannoy
Assist. Direct. Microscopy Facility
JHMI

P.S. Does anyone have the confocal listserver address?




From daemon Fri Mar 1 10:23:17 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Fri, 1 Mar 2002 10:18:08 -0600
Subject: SIMS Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,


Since I have many years of SIMS experience, I responded to the recent
question on this subject directly. I would like to let the group know that
there is a SIMS list at sims-at-sims.arl.army.mil. There is also a Web site at
http://www.simsworkshop.org/default.nclk with lots of information. SIMS is
an expensive technique to own so the user community is small compared to SEM
but if you are looking for low level elemental information its the best
technique for the job.

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu


From daemon Fri Mar 1 10:26:20 2002



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Fri, 01 Mar 2002 10:28:15 -0600
Subject: TEM- Re: Need Info. about Berylium and Berylium-Copper slotted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tony, Tom

I can only imagine Cu-Be grids are used for there superior stiffness
compared to Cu grids. The amount of Be in these alloys is typically 1-2
wt%. The alloy is used to manufacture non-magnetic tools, springs (it can
sustain a greater deflection before permanently deforming than spring steel
up to 200degC) and in the chemical and electrical industry fields where
there is a risk of explosion as the alloy is non-sparking.

Be grids were available for a time although I do not think they are
now. The low atomic number meant that there was no stray x-rays detected
from the grid material. Be is still extensively used in Materials Science
TEM specimen holders (low background) for X-ray analysis because of this.

The dangers of Be and its alloys and compounds can be found on the SIRI
MSDS website:-

siri.org/msds/index.php

Certain precautions are necessary when using Be. Any ingestion of the
metal or its compounds should be avoided. Gloves should be worn at all
times when handling Be or Be containing alloys. There is significantly
less risk of symptoms from Cu-Be alloys although the effects of exposure to
Be is cumulative (and delayed). Be dust is particularly dangerous it
should not be inhaled or ingested, it is a carcinogen causes beryllium
disease, a particularly chronic lung disease, and is an explosion hazard in
air in high concentrations. Be containing parts should not be machined or
filed. Companies machining parts out of Be do so in carefully monitored
glove boxes with special filters to avoid the dust being dispersed in the
atmosphere.

For occasional exposure to Be typical of life in an EM lab, as long as
people are made aware of the dangers and use gloves, do not swallow the
parts (!) and certainly do not take a file to them (!!) they are
safe. Care must be taken however not to lose Be parts and if they are to
be disposed of, they must not just be thrown away!

Regards

Alan

At 04:06 PM 2/28/2002 -0500, Tony Garratt-Reed wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Fri Mar 1 10:37:39 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 1 Mar 2002 10:30:26 -0600
Subject: Luciferase on an LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone out there with experience using a cooled digital camera to
capture the luminescence of luciferase? We are looking at a
macroscopic specimen expressing a luciferase tag using a 20x NA = 0.7
objective and a Sensys camera. I am assuming a don't need to have a
filter in the path since the sample is only source of light. Does
this seem practical? SO far we have had no luck. Any tips or tricks
would be greatly appreciated.
--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Mar 1 11:28:44 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 1 Mar 2002 09:22:20 -0800
Subject: Need Info. about Berylium and Berylium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom;
Beryllium is very potent at hardening copper without significantly
reducing it's electrical conductivity. It is commonly used for springs and
contacts in electrical switches, and is present at such a low concentration
(~0.5%) that it is not considered a hazard. It also does not introduce any
background that would intrude on EDX analysis. We use Be Cu slotted grids as
substrates for 20 micron thick silicon slivers that will be milled in a
focused ion beam tool. The substrate should resist bending because that
would lead to fracture of the silicon. Unalloyed copper would bend too
easily and would not be suitable.

John Mardinly
Intel



-----Original Message-----
} From: "tbargar%unmc.edu"-at-sparc5.microscopy.com
[mailto:"tbargar%unmc.edu"-at-sparc5.microscopy.com]
Sent: Thursday, February 28, 2002 8:31 AM
To: Microscopy-at-sparc5.microscopy.com


An individual new to EM wanted to learn from me how to formvar-carbon coat
slotted grids. The person bought grids made of a Berylium-Copper alloy. I
called the vendor and was warned about the danger of Berylium and it's
vapor. I told the invidual to get copper grids for our use. Now in all my
years in EM I have not worked with Berylium. Would someone out there send
me some information on the hazards of Berylium? Also what applications are
Berylium-Copper or Berylium only grids used for? Any information is
appreciated, thanks.

Tom Bargar
EM Lab, UNMC
tbargar-at-unmc.edu
402-559-7347



From daemon Fri Mar 1 11:34:38 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 01 Mar 2002 09:30:17 -0800
Subject: Re: Need Info. about Berylium and Berylium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tom,
Berylium-copper is an expensive alloy that allows a higher strength and
hardness than most copper alloys while keeping the non-magnetic and
non-sparking properties of copper. I used to have a set of tools for my
ETEC SEM made of Be-Cu. The Be is less than 2.0 weight percent of the alloy
and it is very tightly bound in the copper, so I don't think there is any
danger of Be exposure on normal use or mild heating. The main danger of Be
is the inhalation of BeO in the dust if Be is ground.
At 10:31 AM 2/28/02 -0600, you wrote:
}
} An individual new to EM wanted to learn from me how to formvar-carbon coat
} slotted grids. The person bought grids made of a Berylium-Copper alloy. I
} called the vendor and was warned about the danger of Berylium and it's
} vapor. I told the invidual to get copper grids for our use. Now in all my
} years in EM I have not worked with Berylium. Would someone out there send
} me some information on the hazards of Berylium? Also what applications are
} Berylium-Copper or Berylium only grids used for? Any information is
} appreciated, thanks.
}
} Tom Bargar
} EM Lab, UNMC
} tbargar-at-unmc.edu
} 402-559-7347
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Fri Mar 1 13:33:49 2002



From: Awbrey, Donald :      DonaldAwbrey-at-texashealth.org
Date: Fri, 1 Mar 2002 13:25:30 -0600
Subject: Automated Grid Stainers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear Microscopy List Servers,

I would like to solicit any info about automated grid stainers. I have been
always staining by hand.
Need to know pros and cons about the different ones that are in use. Any
information, such as;
initial cost, size, reproducibility, quality, open/closed system, reagents
utilized, waste disposal,
and etc., etc., will be appreciated.


Thank you TEM netters,

Donald G. Awbrey, HT(ASCP) QIHC
Electron Microscopy / Image Analysis
817-878-5647
donaldawbrey-at-texashealth.org



From daemon Fri Mar 1 13:47:33 2002



From: questions-at-uscensus.info
Date: Fri, 1 Mar 2002 19:40:42 UT
Subject: US Census 2000 data is now available from GeoLytics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


US Census 2000 data is now available from GeoLytics

The U.S. Census Bureau has recently released the first
two major sets of data from the 2000 Census:
Redistricting and the "Short Form" (SF1).

GeoLytics offers these products in two easy-to-use formats
either at the Block level or at the Block Group level
and larger geographies. The data has been compressed
so that it generally fits on 1 disk and it comes with
built-in mapping capabilities, based upon the new
Census 2000 boundaries. With a few quick keystrokes
you can generate full-blown maps or tables. You can
also export the data into a statistical software
package, mapping or other software package.

Below I have listed information and pricing for the
most popular products. For more information about
GeoLytics or our full line of products call us at 1-
800-577-6717 or visit us online at
http://www.uscensus.info


CensuSmart - Includes all four of the data sets
described below. This is our "bundled" product, you
get a copy of each of the data sets (Short Form, Short
Form Blocks, Redistricting, Redistricting Blocks) and
you save a lot of money too. Cost: $995 (all four
items purchased separately would be $2280)
http://www.uscensus.info/census2000.htm

CensusCD 2000 / Short Form - Includes all of the data
released by the U.S. Census Bureau from the SHORT
FORM, including 3,000 variables at the BLOCK GROUP
level and 8,000 variables at the TRACT Level and
above. The program also includes Zip Code Tabulation
Arrays offered by the Census Bureau. Cost: $695
http://www.uscensus.info/census2000.htm

CensusCD 2000 / Short Form BLOCKS - Includes all of
the data released by the U.S. Census Bureau from the
SHORT FORM at the BLOCK level, all 8+ million of them.
Cost $595
http://www.uscensus.info/census2000.htm

CensusCD 2000 / Redistricting - It has the complete
Census 2000 redistricting data along with 1990
redistricting data (for easy timeline comparisons).
Included with the data sets are the 1990 and 2000
mapping boundaries for 19 levels of geography: United
States, Region, Division, State, County, Tract, Block
Group, MSA/CMSA, PMSA, Indian Reservation & Trust
lands, MCD/CCD, Place, Elementary Schools, Secondary
Schools, Unified Schools, 106th Congressional, State
Legislative Districts Upper, State Legislative
Districts Lower and Voter Districts. Cost: $395.
http://www.uscensus.info/census2000.htm

CensusCD 2000 / Redistricting BLOCKS - Includes all of
the Redistricting data released at the BLOCK level,
all 8+ million of them. Cost: $595
http://www.uscensus.info/census2000.htm

We also produce packages for the 1970, 1980 and 1990
Census
http://www.uscensus.info/CensusCD708090/census708090.htm
as well as a 40 Year (1970, 1980, 1990 and
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From daemon Fri Mar 1 13:52:30 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 1 Mar 2002 11:43:26 -0800
Subject: LM: Euparal suppliers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Anyone know where I can buy some Euparal? It's a mounting medium for light
microscopy, often used by entomologists et al. WWW search and other tries
here have turned up nil.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Mar 1 13:57:45 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 01 Mar 2002 11:51:03 -0500
Subject: Re: Need Info. about Berylium and Berylium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 2/28/02 11:31 AM, "tbargar-at-unmc.edu"-at-sparc5.microscopy.com at
"tbargar-at-unmc.edu"-at-sparc5.microscopy.com wrote:

} Also what applications are
} Berylium-Copper or Berylium only grids used for? Any information is
} appreciated, thanks.
}
Dear Tom,
Be grids are used for EDS, since their lower Z means that they scatter
fewer electrons and produce fewer brehmsstrahlung x-rays than other
materials, so they give a very low background count for x-ray analysis.
Also, the only characteristic x-rays they produce are very low energy and do
not interfere with most analyses.
Yours,
Bill Tivol



From daemon Fri Mar 1 13:57:46 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 01 Mar 2002 11:51:03 -0500
Subject: Re: Need Info. about Berylium and Berylium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 2/28/02 4:06 PM, Tony Garratt-Reed at tonygr-at-mit.edu wrote:
}
} This is not a "reply" to the post, but perhaps an extension of the question.
}
} Why would Cu-Be grids be used? Cu-Be is an interesting and quite
} widely-used class of alloys (see http://microstructure.copper.org), but I
} don't see why it would be used for EM grids - unless, that is, the grids we
} loosely call "copper" are in fact all made of Cu-Be?
}
} On the subject of the posting, I can't imagine that Cu-Be would be as
} widely used as it is if it were as toxic as Be-metal. I'm sure the
} alloying must drastically reduce the hazard, but this is not my area of
} expertise.
}
} Tony
}
} }
} } An individual new to EM wanted to learn from me how to formvar-carbon coat
} } slotted grids. The person bought grids made of a Berylium-Copper alloy. I
} } called the vendor and was warned about the danger of Berylium and it's
} } vapor. I told the invidual to get copper grids for our use. Now in all my
} } years in EM I have not worked with Berylium. Would someone out there send
} } me some information on the hazards of Berylium? Also what applications are
} } Berylium-Copper or Berylium only grids used for? Any information is
} } appreciated, thanks.
} }
} } Tom Bargar

Dear Tony & Tom,
Cu-Be is used to make non-magnetic tools, and I'm sure it is used in
preference to copper or bronze because of its strength and toughness. I
suspect that the same qualities would make the Cu-Be grids more durable. Be
metal is toxic in the Be++ form, so the oxide or other compounds are more
toxic than the metal itself. The reason one should be careful handling the
metal is that it can dissolve in the fluid in breaks in the skin, etc., and
be introduced into the blood in the toxic form. Apparently, this does not
happen with the Cu-Be alloy--at least, I've never seen warnings about using
the tools, which often are used by people with cuts or abrasions on the
skin. I'd be interested if anyone knows differently.
Yours,
Bill Tivol



From daemon Fri Mar 1 16:19:41 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 1 Mar 2002 14:12:30 -0800
Subject: Re: Need Info. about Berylium and Berylium-Copper slotted grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Ken;
When I worked at Lockheed, all beryllium alloys that were cut,
ground, or polished had to be prepared in a special lab with numerous
special safety precautions. Beryllium copper alloys (0.5%) were never
subject to these safety rules, and could be cut, ground and polished without
special precautions in any lab.

John Mardinly
Intel



-----Original Message-----
} From: Ken Converse [mailto:qualityimages-at-netrax.net]
Sent: Friday, March 01, 2002 3:38 AM
To: Tony Garratt-Reed; MSA, listserver


Tony,
I can't answer why it would be used, except that Cu-Be is much harder
than Cu. The main danger from Cu-Be is if you grind it or burn it, but
the grids are so small that grinding is fairly unlikely. As used in
non-magnetic tools, etc., you're right. It's very safe.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Tony Garratt-Reed wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This is not a "reply" to the post, but perhaps an extension of the
} question.
}
} Why would Cu-Be grids be used? Cu-Be is an interesting and quite
} widely-used class of alloys (see http://microstructure.copper.org),
} but I don't see why it would be used for EM grids - unless, that is,
} the grids we loosely call "copper" are in fact all made of Cu-Be?
}
} On the subject of the posting, I can't imagine that Cu-Be would be as
} widely used as it is if it were as toxic as Be-metal. I'm sure the
} alloying must drastically reduce the hazard, but this is not my area
} of expertise.
}
} Tony
}
}
} At 10:31 AM 2/28/2002 -0600, you wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } An individual new to EM wanted to learn from me how to formvar-carbon
} } coat
} } slotted grids. The person bought grids made of a Berylium-Copper
} } alloy. I
} } called the vendor and was warned about the danger of Berylium and it's
} } vapor. I told the invidual to get copper grids for our use. Now in
} } all my
} } years in EM I have not worked with Berylium. Would someone out there
} } send
} } me some information on the hazards of Berylium? Also what
} } applications are
} } Berylium-Copper or Berylium only grids used for? Any information is
} } appreciated, thanks.
} }
} } Tom Bargar
} } EM Lab, UNMC
} } tbargar-at-unmc.edu
} } 402-559-7347
}
}
}
} * * * * * * * * * * * * * * * * * * * * * * * * * *
} * Anthony J. Garratt-Reed M.A., D.Phil.
} * MIT, Room 13-1027
} * 77 Massachusetts Avenue
} * Cambridge, MA 02139-4307
} * USA
} * Phone: (617) 253-4622
} * Fax: (617) 258-6478
} *
}
}
}
}




From daemon Fri Mar 1 16:19:41 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 1 Mar 2002 14:11:56 -0800
Subject: Recall: Need Info. about Berylium and Berylium-Copper slotted gri

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mardinly, John would like to recall the message, "Need Info. about Berylium
and Berylium-Copper slotted grids".


From daemon Fri Mar 1 16:40:36 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 01 Mar 2002 16:34:48 -0600
Subject: RE: SEM books on mineral analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A clarification is probably in order. The INCA Crystal software actually
works with scattered electrons (rather than x-rays) to determine the
crystallography of the phase.

EDS is good for identifying most minerals, especially given some background
of the likely candidates. But there are many cases of ambiguity until
crystallographic information is available.

I know that EDS systems are available out there for around $60K. I am
practically certain that would not include the INCA Crystal hardware and
software. I would expect that to nearly double the cost of the system, but
I haven't priced one yet.

Warren

At 08:39 AM 3/1/02 -0500, you wrote:

} Morning Goren (sorry for the missing um- [I'm just a] -lout?),
}
} Now, I'm going to take you at your word and venture into an area
} which is NOT really mine yet.
} Briefly, I would suggest that you consult with Oxford Instruments
} for information on on a product they call "INCA Crystal" (no stock, no
} family and other companies such as Thermo NORAN also have such systems in
} the $50-$100k price range). These systems can be linked to the ICDD
} database for mineral identification and permit collection of diffracted
} X-rays phases within the specimen to determine both phase identification and
} crystal orientation as well as elemental mapping from EDS.
} I hope a practitioner responds, but here is the email of a vendor
} rep: Glenn Kinnear (Oxford): kinnear-at-ma.oxinst.com.
}
} Hope this helps,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} West Chester University
} West Chester, Pennsylvania, USA, 19383
} 610-738-0437
} fmonson-at-wcupa.edu
}
} } ----------
} } From: Göran Axelsson
} } Sent: Thursday, February 28, 2002 6:08 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: SEM books on mineral analysis
} }
} } Hello!
} }
} } I'm looking for a book or some other resources on mineral analysis with
} } EDS.
} }
} } I have read a lot of information on the net about SEM / TEM and other
} } instruments so I have a good theoretical knowledge about the process
} } behind EDS but lacks the practical bit.
} }
} } My background is a MS in physics, some chemistry and some geology.
} } I have had some mineral samples analysed in a Russian lab and now I
} } want to learn more about the practical side.
} } How to interprete the results, how to prepare specimens, which problems
} } could occur....
} }
} } Is there a book or some other information source that you could recommend
} } for me?
} }
} } I will try to visit a lab for some hands on experience during the spring,
} } but I
} } would like to be well prepared so I could get the most out of the visit.
} }
} } My final goal is to find a cheap used SEM with EDS to set up a small lab
} } for
} } mineral analyses.
} }
} } Regards, Göran Axelsson, Sweden




From daemon Fri Mar 1 17:55:47 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Fri, 1 Mar 2002 18:48:15 -0500 (EST)
Subject: Re: LM: Euparal suppliers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jon...

You can order Euparal from Bioquip.

http://www.bioquip.com

Best,

Angela

Angela V. Klaus, Ph.D.

Director, Core Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977


On Fri, 1 Mar 2002, Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} Anyone know where I can buy some Euparal? It's a mounting medium for light
} microscopy, often used by entomologists et al. WWW search and other tries
} here have turned up nil.
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}



From daemon Sat Mar 2 09:31:08 2002



From: Diane G. Miller :      millerd-at-coho.net
Date: Sat, 2 Mar 2002 09:13:11 -0600
Subject: SIMS INFO

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all that responded to my question. I've learned a lot and
appreciate your willingness to share your knowledge.

Sincerely,

Diane


From daemon Sat Mar 2 09:51:25 2002



From: dan-at-isaacson.net ()
Date: Sat, 2 Mar 2002 09:42:27 -0600
Subject: Ask-A-Microscopist:How to observe Cellular Mitosis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dan-at-isaacson.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
March 1, 2002 at 17:27:04
---------------------------------------------------------------------------

Email: dan-at-isaacson.net
Name: Dan Isaacson

Organization: Seattle Central Community College

Education: Undergraduate College

Location: Seattle, Washington

Question: I live in Seattle and I was wondering what would be the
best place/microscope/technique to observe cellular mitosis. More
specifically I'm looking to observe the mitoic spindles through
interphase, prophase, prometaphase, metaphase, anaphase, and
telophase. Considering the size of spindles and the microtubule
fibers that connect to the chromosomes it may be very difficult to
observe. But any help you can give me would be greatly appriciated.

Thanks,
Dan Isaacson

---------------------------------------------------------------------------


From daemon Sat Mar 2 10:36:16 2002



From: David Burton :      dburton-at-nwlink.com
Date: Sat, 2 Mar 2002 08:32:27 -0800
Subject: Cooled chamber?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

We need a cooled chamber large enough to hold a multiwell plate, 3"X6"
(approximately), able to hold the temperature at 4 degrees C. This chamber
will be used with a stereo zoom microscope at relatively high power and we
must be able to pipette the multiwell plate through a port in the chamber.
If you have any information on manufacturers of a device like this, or know
of someone who has built one, please share the information with us. We
would like to avoid reinventing the wheel on this project and hope somebody
has done this already. We are are about to start fabricating one because we
haven't found a source for one.

We are able to make any modifications needed to make a chamber that is
close to what we need. This research previously has been done
with the microscope and researcher inside a large refrigerater! Burr...

Thanks for your help.
Dave Burton
University of Washington, Seattle




From daemon Sat Mar 2 11:03:22 2002



From: James Talbot :      james-at-ktgeo.com
Date: Sat, 2 Mar 2002 10:54:44 -0600
Subject: Re: SEM books on mineral analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Goran-

The "SEM Petrology Atlas" by Joann Welton is out of print. I tried to find a
copy several months ago but was unable to. I checked Amazon.com today and
there is one copy available from a used book dealer for US $371. A bit pricy
but if you plan on looking at a lot of rocks, especially sedimentary rocks,
this is an excellent reference. You might want to search Amazon.com to find
some more recent (and less expensive) books.

Most of my work is X-ray diffraction on rocks but I do some SEM work. The
sample prep is pretty simple. If you have any specific questions, feel free
to contact me off-list.

Sincerely,
James Talbot

K/T GeoServices, Inc.
Bulk and Clay Mineralogy by X-ray Diffraction
(940) 597-9076
web site: http://www.ktgeo.com/


Lou Ross wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Goran,
}
} The book I have used for years is called "SEM Petrology Atlas" by Joann E.
} Welton. It was published in 1984 by the American Association of Petroleum
} Geologists (Tulsa, OK 74101) as part of the Methods in Exploration Series.
} Not sure if it still available, but the ISBN # is 0-89181-653-4.
}
} It contains images and EDS spectra of a variety of minerals - silicates,
} carbonates, phosphates, halides, sulfides, sulfates, and oxides.
}
} Hope this helps,
} Lou Ross
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello!
} }
} } I'm looking for a book or some other resources on mineral analysis with
} } EDS.
} }
} } I have read a lot of information on the net about SEM / TEM and other
} } instruments so I have a good theoretical knowledge about the process
} } behind EDS but lacks the practical bit.
} }
} } My background is a MS in physics, some chemistry and some geology.
} } I have had some mineral samples analysed in a Russian lab and now I
} } want to learn more about the practical side.
} } How to interprete the results, how to prepare specimens, which problems
} } could occur....
} }
} } Is there a book or some other information source that you could
} } recommend
} } for me?
} }
} } I will try to visit a lab for some hands on experience during the
} } spring, but I
} } would like to be well prepared so I could get the most out of the visit.
} }
} } My final goal is to find a cheap used SEM with EDS to set up a small lab
} } for
} } mineral analyses.
} }
} } Regards, Göran Axelsson, Sweden
}
} Senior Electron Microscope Specialist
} Electron Microscopy Core Facility
} W136 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211-5120
} (573) 882-4777, fax 884=5414
} email: rosslm-at-missouri.edu
} web: www.biotech.missouri.edu/emc


From daemon Sat Mar 2 11:05:11 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Sat, 2 Mar 2002 13:29:09 -0330
Subject: SEM: Centaurus detector questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have questions pertaining to practical applications for this BSE/CL
detector. Would any of you who have practical experience please contact me
directly.

genuinely ... michael shaffer :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com



From daemon Sat Mar 2 11:05:12 2002



From: michael shaffer :      michael-at-shaffer.net
Date: Sat, 2 Mar 2002 13:28:53 -0330
Subject: SEM: Centaurus detector questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have questions pertaining to practical applications for this BSE/CL
detector. Would any of you who have practical experience please contact me
directly.

genuinely ... michael shaffer :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com



From daemon Sat Mar 2 16:48:47 2002



From: Joanne Whallon :      whallon-at-pilot.msu.edu
Date: Sat, 02 Mar 2002 17:41:16 -0500
Subject: Re: Need Info. about Beryllium and Beryllium-Copper slotted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom --

Many years ago (1986) we were attempting to clean Formvar coating and epoxy
resin sections from slotted beryllium grids. Since, for safety reasons,
acid could not be used to clean beryllium, we were following the
suppplier's suggestion to use either acetone or ethanol. When an initial
wash in acetone did not do a satisfactory job, the grids were placed in
100% ethanol. Shortly thereafter, examination of the grids under a
dissecting microscope revealed distinct signs of corrosion: holes appeared
in one grid, and pieces broke from the edges of two others.

We immediately contacted the supplier, who immediately called the
manufacturer, who was of the opinion that the formaldehyde in Formvar, in
conjunction with an organic solvent, could indeed initiate corrosive action
on the beryllium. Proper disposal consisted of pouring the grids and the
ethanol solution onto filter paper in a funnel; the wet filter paper with
the grids on it was placed in a plastic bag (to prevent drying out and
subsequent release of dust) for pick-up by the biological safaety office,
and the liquid was poured down the sink.

At that time beryllium itself was not considered nearly as toxic as it had
once been, but beryllium salts were still very bad news. As a result of
our experience, the supplier (Ted Pella) decided to suggest to all
customers that NO attempt be made to clean beryllium grids, but that they
be discarded in a safe manner after use.

Joanne Whallon









From daemon Sun Mar 3 00:36:45 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 03 Mar 2002 00:19:21 -0500
Subject: Be TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Alan Nicholls wrote:
==========================================
I can only imagine Cu-Be grids are used for there superior stiffness
compared to Cu grids. The amount of Be in these alloys is typically 1-2
wt%. The alloy is used to manufacture non-magnetic tools, springs (it can
sustain a greater deflection before permanently deforming than spring steel
up to 200degC) and in the chemical and electrical industry fields where
there is a risk of explosion as the alloy is non-sparking.

Be grids were available for a time although I do not think they are now.
The low atomic number meant that there was no stray x-rays detected from
the grid material. Be is still extensively used in Materials Science TEM
specimen holders (low background) for X-ray analysis because of this.
===========================================
We might be comparing apples with oranges here.

What is referred to as "copper/beryllium alloy" indeed is mainly copper with
a little bit of beryllium added. I have worked in the M&M field for more
years than I will admit and I have never heard of anyone making either grids
or planchettes out of that alloy. After all, it would be self-defeating and
would serve no useful purpose in EM.

There are alloys of Be that are 98 and 99% Be and the non-Be content can be
Cu and/or other impurities. Over the years, grids and planchettes have been
offered of the 98 and 99% purity alloys, and they were somewhat cheaper than
the higher purity 99.8% Be that have been offered now for some years by SPI
Supplies. If you have grids where no statement of purity was given, we
believe that you can probably assume they were 98% or possibly 99% but not
the 99.8% level.

At one time, and they might still be offered, were essentially copper grids
that had a sputter coated layer of Be. I think the theory was that the Be
coating approach could lead to a lower priced product that could do the same
thing. We always had the idea that they did not work very well in terms of
keeping Cu lines out of the EDS spectra. That might not be the experience of
others, however. We also heard reports of the Be layer flaking off as the
grid was flexed, which could lead to an inhalation hazard.

A full range of 99.8% purity Be grids is described on URL
http://www.2spi.com/catalog/grids/beryl.html

They are very much still available off-the-shelf from SPI Supplies. For
those who are in institutions that have banned Be in any form, the above URL
mentions and provides links to product alternatives.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sun Mar 3 01:55:05 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 03 Mar 2002 00:19:18 -0500
Subject: Beryllium grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Joanne Whallon wrote:
=================================================================
Many years ago (1986) we were attempting to clean Formvar coating and epoxy
resin sections from slotted beryllium grids. Since, for safety reasons,
acid could not be used to clean beryllium, we were following the supplier's
suggestion to use either acetone or ethanol. When an initial wash in
acetone did not do a satisfactory job, the grids were placed in 100% ethanol
. Shortly thereafter, examination of the grids under a dissecting
microscope revealed distinct signs of corrosion: holes appeared in one grid,
and pieces broke from the edges of two others.

We immediately contacted the supplier, who immediately called the
manufacturer, who was of the opinion that the formaldehyde in Formvar, in
conjunction with an organic solvent, could indeed initiate corrosive action
on the beryllium. Proper disposal consisted of pouring the grids and the
ethanol solution onto filter paper in a funnel; the wet filter paper with
the grids on it was placed in a plastic bag (to prevent drying out and
subsequent release of dust) for pick-up by the biological safety office, and
the liquid was poured down the sink.

At that time beryllium itself was not considered nearly as toxic as it had
once been, but beryllium salts were still very bad news. As a result of our
experience, the supplier (Ted Pella) decided to suggest to all customers
that NO attempt be made to clean beryllium grids, but that they be discarded
in a safe manner after use.
==========================================================
A minute or less in an oxygen plasma, such as what is produced in the SPI
Plasma Prep II plasma etcher will "etch" away anything organic in the way of
a TEM thickness section. I am talking about seconds. Because of the
isotropic nature of the etching process in the Plasma Prep II (as opposed to
anisotropic etchers), both sides seemed to get cleaned, although if it was
me, I would try to put the section side up.

One never knows for sure of course, but the etching rate with oxygen on
Beryllium metal would be essentially zero when compared to anything organic.


And while it might be a digression from the main thread of the topic, let me
point out that the hazard is an inhalation hazard, namely beryllium oxide
(BeO). Beryllium metal is about as inert and innocuous as anything you can
find. A dangerous situation does not arise just have having the grid (or
planchette) sitting there, or for that matter, putting the grid in the TEM
(or SEM) but from the processing of the item. For example, those who might
try "repolishing" a Be planchette take on an especially high risk because if
their polishing table is allowed to dry out, there could result a dry powder
that has become BeO and it would indeed become a hazard if the polishing
media was dislodged and the entrapped particulates became air borne. I have
not heard of an analogous situation with regard to the cleaning,
mechanically, the grids.

If one was concerned about beryllium in any form exiting the mechanical pump
being used with a plasma etcher, and if a standard oil mist filter was
deemed to not be enough protection, then the pump can be vented directly to
the laboratory's fume exhaust system (or operated inside of the fume hood).

Finally, contrary to conventional wisdom in M&M land, many of these products
, including Be grids are not all the same, especially with regard to non-
breyllium content, such as copper. Additional information can be found on
URL
http://www.2spi.com/catalog/grids/note.html

The chemical resistance of beryllium alloyed with copper will be
considerably less, and therefore explaining the pitting, than the higher
purity preferred by those who use these kinds of grids on a regular basis.
The point is that your experience with the etching suggests the non-
beryllium content of the grids was high and such experience could not be
expected to be reproduced by someone using the higher purity (and more
expensive beryllium foil) that is used in the making of the higher Be
content grids.

Disclaimer: SPI Supplies is a supplier of high purity beryllium grids so we
would have a vested interest in seeing more people use SPI Supplies® brand
of beryllium grids than grids offered by others.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sun Mar 3 12:31:09 2002



From: lisa.monaco-at-msfc.nasa.gov ()
Date: Sun, 3 Mar 2002 12:11:48 -0600
Subject: Ask-A-Microscopist: imaging of transparent cubic crystals in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lisa.monaco-at-msfc.nasa.gov) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
March 1, 2002 at 13:27:42
---------------------------------------------------------------------------

Email: lisa.monaco-at-msfc.nasa.gov
Name: lisa monaco

Organization: MSFC

Education: Graduate College

Location: Hunstville Alabama

Question: What would be the some of the best optical techniques for
high resolution imaging of transparent cubic crystals in solution.

The are 20 microns on edge, at high resolution (i.e., we want to be
able to make
measurements on crystal dimensions within about 5-10 microns) and they are
in transparent solution from which they grew. In some cases, just a phase
separation (for example SCN crystals out of SCN solution)


---------------------------------------------------------------------------


From daemon Mon Mar 4 10:02:09 2002



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Mon, 04 Mar 2002 09:59:20 -0600
Subject: Re: Beryllium grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chuck

I think you need to look at the International Chemical Safety Card (ICSC)
for Beryllium (not the oxide) in POWDER form. The main points are:-

Fire - combustable
Explosion - finely dispersed particles form explosive mixtures in air

EXPOSURE - PREVENT DISPERSION OF DUST! AVOID ALL CONTACT! (their
capitals). Effects of short-term exposure to high quantities of the dust
are irritation of the respiratory tract. Inhalation of the dust or fumes
causes chemical pneumonitis. Exposure may result in death. Effects may be
delayed.
(The Chicago Tribune ran another article yesterday about the effects of Be
machining in the armed forces and nuclear industries. Effects of exposure
may not be obvious for 20-35 years)

Repeated or long term contact may cause skin sensitization. Lungs may be
affected resulting in chronic beryllium disease. Be powder is carcinogenic
and should not be ingested. Finally Be powder is very toxic to aquatic
organisms.

I agree with you that Solid "Beryllium metal is about as inert and
innocuous as anything you can find" and that using Be grids, Be planchets
and Be low background holders is safe as long as "people are made aware of
the dangers and use gloves, do not swallow the parts (!) and certainly do
not take a file to them (!!)" as I said in my original posting.

However, both the metal and its compounds in powder/ particle form are
dangerous and should not be released to the environment in air or
water. As it says in the spillage disposal notes in the ICSC "Do NOT let
this chemical enter the environment"

Regards

Alan


At 12:19 AM 3/3/2002 -0500, Garber, Charles A. wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Mon Mar 4 10:09:10 2002



From: Lou Ross :      RossLM-at-missouri.edu
Date: Mon, 4 Mar 2002 10:05:41 -0600
Subject: APEX WDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I would like to hear from any APEX WDS owners out there to see how
many are still active (maybe we can establish a user's group?) or if
any inactive systems are available for parts. Please respond to me
directly.

Thanks,
Lou Ross
--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.biotech.missouri.edu/emc


From daemon Mon Mar 4 13:29:22 2002



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Mon, 04 Mar 2002 15:10:44 -0300 (ADT)
Subject: TEM-Protein-A

Contents Retrieved from Microscopy Listserver Archives
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Hi everybody!
Does anybody know if Protein A reacts with antibodies of
non-human primates (ie. macaque and baboon)?
Thanks
Dorota


From daemon Mon Mar 4 14:20:29 2002



From: Edward_Principe-at-amat.com
Date: Mon, 4 Mar 2002 12:14:23 -0800
Subject: RE: SIMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



There are several permutations, most covered nicely, just a thought or two:

I believe you could also consider a ToF-SIMS as a "ion beam microscope" but
not as an "ion beam electron microscope" since the former generates highly
surface sensitive ion-based chemical information (including images) and the
latter implies electron images generated from ions (ions in and electrons
out). The contrast mechanism and the image information volume would be
different from standard electron images.

ToF-SIMS utilizes a FIB ion beam and a mass/charge analyzer to obtain
information from ~submonolayer regions because the ion beam flux is in the
static regime. It can produce "chemical signature images" with a spatial
resolution that varies, but can reach sub-micron.

Quadrapole SIMS detectors have been installed on standard dual beam FIB
instruments, the primary benefit is enhanced detection limits and speed
with respect to some other forms of analysis available in such systems.
Fred Stevie (now at the university of North Carolina, I think...but
definitely not with Agere(lucent) any longer) has done quite a bit in that
area.

Regards,
Ed




Gary Gaugler {gary-at-gaugler.com} on 03/01/2002 07:28:51 AM


To: "Malis, Tom" {malis-at-nrcan.gc.ca}
cc: MSA listserver {Microscopy-at-sparc5.microscopy.com}


Could be she was refering to SIMS and FIB. Based on
my experience with FEI FIB (old model 611), it has poor
imaging resolution. Their newer models, like the 830,
are as you say, dual beam--ion and electron. The electron beam
is used for imaging while the ion beam is used for
micro machining, etc. FIBs are great for making
microcircuit cross sections and changing runners
on the planar area of a chip.

Supposedly, the FEI dual beam FIB will accept a
SIMS "detector." So it would do a whole bunch of
good tasks. Maybe there is only one spare port.
Either a SIMS detector or x-ray detector could
be fitted.

gary g.


At 05:05 AM 3/1/2002, you wrote:
} You are correct in your 'secret cult' image, Gary. SIMS is largely a
} well-kept secret of both the geoscience and semiconductor communities,
with
} the vast majority of materials scientists being unaware of its
capabilities
} in trace element detection (including even hydrogen), sputter depth
} profiling, elemental imaging, isotope ratio age-dating and so forth. We
} here in Ottawa at a federal materials science-oriented lab have had a SIMS
} for nearly 15 years as a complement to our SEM, TEM and electron
microprobe.
} Like our XPS and Auger, however, the SIMS is not used nearly as much as
the
} EMs, but clients in the above two materials communities use it regularly.
} Let me note that the 'new kid on the block' in the SIMS community is
} something called a nanoSIMS, with a 50 nm beam (as opposed to the
micron-ish
} beam of a regular SIMS) and simultaneous detection of up to 5 elements
(much
} like a microprobe with WDX detectors). To date there are only two in
North
} America, no surprise given the ~$2M+ US price tag.
}
} However, I believe that Diane was referring to the differences between a
} SIMS and a focused ion beam (FIB) system, which is essentially a scanning
} ion microscope. In a FIB a much higher energy ion beam (30-50 keV as
} opposed to only several keV for a SIMS) is focused by electrostatic lens
} down to as little as 5 nm and scanned as in an SEM. The ion beam
generates
} both secondary ions and secondary electrons, which can be captured to form
} the corresponding two types of images. Generally speaking, the resolution
} is intermediate between an LaB6 emitter SEM and a field emission (FE) SEM.
} The use of an electron flood gun permits good imaging of insulating
} materials. The most important difference between a SIMS and a FIB is
that,
} while the former uses the scanned beam to sputter a crater for depth
} profiling, the latter uses its more energetic beam to accomplish in-situ
} 'micromachining'. Thus FIBs had their inception in the microelectronics
} community to conduct fine-scale repairs on devices. More recently, they
} have impacted seriously on general materials science via use of this
} micromachining capability to prepare parallel-sided thin sections for TEM
in
} various ways. Finally, alas, FIBs have no analytical (EDXS) capability to
} date, save for a few models that combine both a ion beam column and an
} electron beam column (thus called dual beam FIBS), wherein an EDXS
detector
} can be used in conjunction with the latter.
}
} Attendees of M&M 2002 in Quebec City should check out the FIB session
} chaired by Lucille Gianuzzi and Mike Phaneuf. I guarantee you'll be
} impressed.
}
} Tom
}
} Tom Malis
} Group Leader - Characterization
} Materials Technology Laboratory
} Natural Resources Canada (Govt. of Canada)
} 568 Booth St., Ottawa, Canada
}
} } ----------
} } From: Gary Gaugler
} } Sent: Thursday, February 28, 2002 6:20 PM
} } To: Diane G. Miller
} } Cc: MSA listserver
} } Subject: Re: SIMS
} }
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } Did not see any response yet so I'll give it a try.
} }
} } A good reference for SIMS is Wilson, R., Stevie, F.
} } and Magee, C. (1989). Secondary ion mass spectrometry.
} } New York: John wiley & Sons. ISBN 0-471-51945-6
} }
} } Ion beam microscopy is a mode which is available in
} } some, if not all (not sure) SIMS units. The distinction
} } is made between depth profiles, side wall ion contributions
} } and other effects. Large area ratios are typically
} } required for probe mode to exclude secondary ions
} } from the sidewalls when the beam is in the center
} } of the crater. Alternatively, secondary ions are
} } rejected outside the center of the crater "with an
} } aperture for the ion microscope mode" (p. 1.5-1).
} }
} } I haven't seen much other SIMS reference material either.
} } Maybe it is a secret cult?
} }
} } gary g.
} }
} }
} } At 04:57 PM 2/27/2002, you wrote:
} }
} } } Hello All,
} } }
} } } I need some information. I hope I will get some responses from you. I
} } was
} } } wondering what the difference is between a SIMS Secondary Ion Mass
} } } Spectrometer and an Ion Beam Electron Microscope. Please excuse my
} } } ignorance, but I've tried looking on the web, and I haven't found the
} } } explanation that I need.
} } }
} } } Any help would be appreciated.
} } }
} } } Thank you in Advance.
} } }
} } } Diane
} } }
} } } {mailto:millerd-at-coho.net} millerd-at-coho.net
} } }
} }
} }






From daemon Mon Mar 4 14:34:36 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Mon, 04 Mar 2002 15:27:49 -0500
Subject: Spiffy Freeware for H & E imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I just found out about a spiffy new freeware called IrfanView (pronounced earfan-view).

We were having trouble capturing H&E colors using PhotoShop. I then heard from the guy who does tech support for the RGB camera that we use about IrfanView. It is GREAT! We capture our images and the colors are pretty true.

We haven't figured it all out yet, so we still use PhotoShop for other stuff, but I recommend that you take a look at the program

Go to www.irfanview.com

Has anybody else used this software? What's your opinion?


Paula :-)

p.s. I have no connection to this product I'm just a happy user.


Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Mon Mar 4 15:58:53 2002



From: Louis Kerr :      lkerr-at-mbl.edu
Date: Mon, 04 Mar 2002 16:59:08 -0500
Subject: Cover slips with grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

Can anyone suggest a source of cover slips for light microscopy that
have some type of grid pattern on the surface? Ideally we are looking
for No. 1.5 square cover slips.

Thanks,
Louie

--
Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITE:
http://www.mbl.edu/
http://www.courses.mbl.edu/


From daemon Mon Mar 4 16:56:54 2002



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Tue, 05 Mar 2002 09:49:50 +1100
Subject: Re: Spiffy Freeware for H & E imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I get evangelical about this one too - as a fast-loading basic 8/24-bit
image handling package its great.

Sally Stowe


} } } "Paula Sicurello" {patpxs-at-gwumc.edu} 03/05/02 07:27AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Listers,

I just found out about a spiffy new freeware called IrfanView (pronounced
earfan-view).

We were having trouble capturing H&E colors using PhotoShop. I then heard
from the guy who does tech support for the RGB camera that we use about
IrfanView. It is GREAT! We capture our images and the colors are pretty
true.

We haven't figured it all out yet, so we still use PhotoShop for other
stuff, but I recommend that you take a look at the program

Go to www.irfanview.com

Has anybody else used this software? What's your opinion?


Paula :-)

p.s. I have no connection to this product I'm just a happy user.


Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax




From daemon Mon Mar 4 17:10:35 2002



From: Mark Williamson :      mjw4b-at-virginia.edu
Date: Mon, 04 Mar 2002 17:59:09 -0500
Subject: XRD Texture Resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers-
I am trying to determine the resolution using x-ray diffraction for thin
film texture determinations, (i.e. smallest grain size and film thickness
which can be investigated). When I read books like Cullity, it appears
that the resolution will be limited to about 100 nm based on diffraction
peak broadening due to the particle size, (although I have not found any
books published recently on the technique, those in the 1980's, quote the
spatial resolution as high as 500nm). However, I seem to recall discussions
of XRD measurements on films down to 5nm in thickness. I have never used
this technique, but my suspicion is that it would be very difficult to
deconvolve the substrate from the film. I would be greatful to hear any
comments on the 'practical resolution' of this technique.
Thank You,
Mark Williamson
Mark Williamson
mjw4b-at-virginia.edu



From daemon Mon Mar 4 17:11:36 2002



From: Erica Steadman :      esteadman-at-pointbio.com
Date: Mon, 4 Mar 2002 15:06:08 -0800
Subject: TEM- Need TEM done on a biologic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a company located in the San Francisco Bay Area (California) that performs TEM's on biologics?
I need a TEM of an air filled double-walled microsphere.

Thank You,
Erica Steadman
POINT Biomedical Corp.
San Carlos, California


From daemon Mon Mar 4 18:11:31 2002



From: n-alem-at-northwestern.edu ()
Date: Mon, 4 Mar 2002 18:02:25 -0600
Subject: Ask-A-Microscopist: TEM Sample out of the eutectic Ceramic oxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (n-alem-at-northwestern.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
March 4, 2002 at 12:54:52
---------------------------------------------------------------------------

Email: n-alem-at-northwestern.edu
Name: Nasim Alem

Organization: Northwestern University

Education: Graduate College

Location: Evanston, IL, USA

Question: I am trying to make a TEM Sample out of the eutectic
Ceramic oxide NiO/ZrO2. I was wondering what would be the most
appropriate paste and polishing paper to mechanically thin down the
sample.

I have been trying fine SiC paper, however it is not very effective
in thining down the sample.
The sample is also very brittle. I have had crack initiation and
prpapagation on the surface of the sample, while using coarse SiC
paper.


---------------------------------------------------------------------------


From daemon Mon Mar 4 18:11:32 2002



From: w-ding-at-northwestern.edu ()
Date: Mon, 4 Mar 2002 18:02:04 -0600
Subject: Ask-A-Microscopist: Hitachi S 4500 SEM Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (w-ding-at-northwestern.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
March 4, 2002 at 17:17:11
---------------------------------------------------------------------------

Email: w-ding-at-northwestern.edu
Name: Weiqiang Ding

Organization: Northwestern univ

Education: Graduate College

Location: Evanston,IL, US

Question: I am a user of Hitachi S 4500 SEM.
I want to know the relationship of the second electron detector
output signal and CRT display signal. As you know, the SEM has
different scan mode,including TV mode, and more slow modes. I want to
know the time for each line scan on CRT and also for whole frame
corresponding to the electron beam scan on sample. Is there any
relationship or simple equations for calculation.

Thanks.


---------------------------------------------------------------------------


From daemon Mon Mar 4 18:27:25 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 04 Mar 2002 16:21:55 -0800
Subject: Re: Spiffy Freeware for H & E imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am using IrfanViewer for many years since their first release. I am
using it as a 'general-purpose' viewer for the most image formats. It's
very quick and has a lot of very nice functions. It's small and very
efficient. It's one of the best viewers I ever seen. No interest in
company, but happy user. Sergey

At 12:27 PM 3/4/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Mar 4 18:40:04 2002



From: Mark Williamson :      mjw4b-at-virginia.edu
Date: Mon, 04 Mar 2002 19:29:31 -0500
Subject: XRD Texture Resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} Dear Listers-
} I am trying to determine the resolution using x-ray diffraction for
} thin film texture determinations, (i.e. smallest grain size and film
} thickness which can be investigated). When I read books like Cullity, it
} appears that the resolution will be limited to about 100 nm based on
} diffraction peak broadening due to the particle size, (although I have
} not found any books published recently on the technique, those in the
} 1980's, quote the spatial resolution as high as 500nm). However, I seem
} to recall discussions of XRD measurements on films down to 5nm in
} thickness. I have never used this technique, but my suspicion is that it
} would be very difficult to deconvolve the substrate from the film. I
} would be greatful to hear any comments on the 'practical resolution' of
} this technique.
} Thank You,
} Mark Williamson

Mark Williamson
mjw4b-at-virginia.edu



From daemon Tue Mar 5 02:44:30 2002



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Tue, 05 Mar 2002 10:35:48 +0200
Subject: Re: Spiffy Freeware for H & E imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I also find Irfanview very useful, and use it as my default image
viewer. It is small and fast with a good number of facilities. Batch
conversion (and / or file renaming) from one format to another is
especially efficient. It displays, manipulates and saves a large
variety of image formats. Setting up a slideshow is quick.
Really useful software.
The freeware version is for home use, the author requests a
registration fee of 10 US dollars for non-personal use.

(No connection with the author or program, just a satisfied user).


Jan Coetzee
Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/academic/electron/emunit1.htm




Paula Sicurello wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Listers,
}
} I just found out about a spiffy new freeware called IrfanView (pronounced earfan-view).
}
} We were having trouble capturing H&E colors using PhotoShop. I then heard from the guy who does tech support for the RGB camera that we use about IrfanView. It is GREAT! We capture our images and the colors are pretty true.
}
} We haven't figured it all out yet, so we still use PhotoShop for other stuff, but I recommend that you take a look at the program
}
} Go to www.irfanview.com
}
} Has anybody else used this software? What's your opinion?
}
} Paula :-)
}
} p.s. I have no connection to this product I'm just a happy user.
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax


From daemon Tue Mar 5 03:31:52 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Tue, 05 Mar 2002 10:23:52 +0100
Subject: cover slips and grid pattern

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I read your email about coverslips with grids on the microscopy. I
believe a good place to look for grids is the website of "Edmund Optics"
(http://www.edmundoptics.com/). They have several types of grids for
micrscopy.

If the grid is needed for calibrating a digital microscopy setup, this
can also be done without a grid if you know some of the "physical"
properties of the CCD-camera and the digitizer.

Best regards,

Peter

P.S. I have no commercial relation with Edmund Optics, I only know about
their grids because I have used some of them for my own work.

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

==========================================================

=======================================================
Louis Kerr wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello all,
}
} Can anyone suggest a source of cover slips for light microscopy that
} have some type of grid pattern on the surface? Ideally we are looking
} for No. 1.5 square cover slips.
}
} Thanks,
} Louie
}
} --
} Louie Kerr
} Research and Education Support Coordinator
} Marine Biological Laboratory
} 7 MBL Street
} Woods Hole, MA 02543
} 508-289-7273
} 508-540-6902 (FAX)
} 508-292-0289 (Cell phone)
}
} VISIT OUR WEB SITE:
} http://www.mbl.edu/
} http://www.courses.mbl.edu/


From daemon Tue Mar 5 06:57:08 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 4 Mar 2002 13:05:36 -0500
Subject: RE: SEM books on mineral analysis

Contents Retrieved from Microscopy Listserver Archives
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Thanks Warren,
I am reading the literature again, hoping the RAM works better next
time. I knew I was going to get in trouble, but, nothing ventured nothing
learned. Now I understand the purpose of the CCD camera - - - - I think!

No confusion about the cost, and it is NOT included with EDS.

Are all the phase identification/analysis systems the same?

Regards,

Fred

} ----------
} From: Warren E Straszheim
} Sent: Friday, March 1, 2002 5:34 PM
} To: Microscopy-at-sparc5.microscopy.com
} Cc: Göran Axelsson
} Subject: RE: SEM books on mineral analysis
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} A clarification is probably in order. The INCA Crystal software actually
} works with scattered electrons (rather than x-rays) to determine the
} crystallography of the phase.
}
} EDS is good for identifying most minerals, especially given some
} background
} of the likely candidates. But there are many cases of ambiguity until
} crystallographic information is available.
}
} I know that EDS systems are available out there for around $60K. I am
} practically certain that would not include the INCA Crystal hardware and
} software. I would expect that to nearly double the cost of the system, but
}
} I haven't priced one yet.
}
} Warren
}
} At 08:39 AM 3/1/02 -0500, you wrote:
}
} } Morning Goren (sorry for the missing um- [I'm just a] -lout?),
} }
} } Now, I'm going to take you at your word and venture into an area
} } which is NOT really mine yet.
} } Briefly, I would suggest that you consult with Oxford
} Instruments
} } for information on on a product they call "INCA Crystal" (no stock, no
} } family and other companies such as Thermo NORAN also have such systems in
} } the $50-$100k price range). These systems can be linked to the ICDD
} } database for mineral identification and permit collection of diffracted
} } X-rays phases within the specimen to determine both phase identification
} and
} } crystal orientation as well as elemental mapping from EDS.
} } I hope a practitioner responds, but here is the email of a
} vendor
} } rep: Glenn Kinnear (Oxford): kinnear-at-ma.oxinst.com.
} }
} } Hope this helps,
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging
} } West Chester University
} } West Chester, Pennsylvania, USA, 19383
} } 610-738-0437
} } fmonson-at-wcupa.edu
} }
} } } ----------
} } } From: Göran Axelsson
} } } Sent: Thursday, February 28, 2002 6:08 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: SEM books on mineral analysis
} } }
} } } Hello!
} } }
} } } I'm looking for a book or some other resources on mineral analysis
} with
} } } EDS.
} } }
} } } I have read a lot of information on the net about SEM / TEM and other
} } } instruments so I have a good theoretical knowledge about the process
} } } behind EDS but lacks the practical bit.
} } }
} } } My background is a MS in physics, some chemistry and some geology.
} } } I have had some mineral samples analysed in a Russian lab and now I
} } } want to learn more about the practical side.
} } } How to interprete the results, how to prepare specimens, which
} problems
} } } could occur....
} } }
} } } Is there a book or some other information source that you could
} recommend
} } } for me?
} } }
} } } I will try to visit a lab for some hands on experience during the
} spring,
} } } but I
} } } would like to be well prepared so I could get the most out of the
} visit.
} } }
} } } My final goal is to find a cheap used SEM with EDS to set up a small
} lab
} } } for
} } } mineral analyses.
} } }
} } } Regards, Göran Axelsson, Sweden
}
}
}
}


From daemon Tue Mar 5 06:57:10 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 4 Mar 2002 12:57:34 -0500
Subject: RE: Ask-A-Microscopist:How to observe Cellular Mitosis?

Contents Retrieved from Microscopy Listserver Archives
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Hi Dan,

Differential Interference Contrast (DIC)! or barring that, phase coupled
with timed-sequence photography or just a plain video camera. Normal cells
get thru the process in under 30min.

Get on the net and query Google with the following: "mitosis university of
washington".

Look what I found in just a minute:

http://www.science.smith.edu/departments/Biology/Bio112/mitosislab.html

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


} ----------
} From: dan-at-isaacson.net
} Sent: Saturday, March 2, 2002 10:42 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist:How to observe Cellular Mitosis?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (dan-at-isaacson.net) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
} March 1, 2002 at 17:27:04
} --------------------------------------------------------------------------
} -
}
} Email: dan-at-isaacson.net
} Name: Dan Isaacson
}
} Organization: Seattle Central Community College
}
} Education: Undergraduate College
}
} Location: Seattle, Washington
}
} Question: I live in Seattle and I was wondering what would be the
} best place/microscope/technique to observe cellular mitosis. More
} specifically I'm looking to observe the mitoic spindles through
} interphase, prophase, prometaphase, metaphase, anaphase, and
} telophase. Considering the size of spindles and the microtubule
} fibers that connect to the chromosomes it may be very difficult to
} observe. But any help you can give me would be greatly appriciated.
}
} Thanks,
} Dan Isaacson
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Tue Mar 5 08:00:27 2002



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Tue, 5 Mar 2002 07:52:14 -0600
Subject: RE: Spiffy Freeware for H & E imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Paula et al.

At the risk of redundancy I can't help but add to the praise for Irfanview.


I've wished vainly for a few years for a replacement for the classic Mac
'GraphicConverter' sofwtare, and this appears to have all the elements
(possibly more - I'm just getting acquainted). It's free (yes, doesn't
self-destruct after 30 days, and doesn't bombard you with registration
warnings). It's also fast and has numerous useful features (support of 16
bit grey scale images; full screen viewing mode; also check out the feature
which allows you to scroll through the entire contents of a directory by
simply hitting spacebar - nifty!).

Wharton

*********************************************************************
Wharton Sinkler
UOP LLC
Des Plaines, IL 60017-5017




} -----Original Message-----
} From: Paula Sicurello [SMTP:patpxs-at-gwumc.edu]
} Sent: Monday, March 04, 2002 2:28 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Spiffy Freeware for H & E imaging
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Listers,
}
} I just found out about a spiffy new freeware called IrfanView (pronounced
} earfan-view).
}
} We were having trouble capturing H&E colors using PhotoShop. I then heard
} from the guy who does tech support for the RGB camera that we use about
} IrfanView. It is GREAT! We capture our images and the colors are pretty
} true.
}
} We haven't figured it all out yet, so we still use PhotoShop for other
} stuff, but I recommend that you take a look at the program
}
} Go to www.irfanview.com
}
} Has anybody else used this software? What's your opinion?
}
}
} Paula :-)
}
} p.s. I have no connection to this product I'm just a happy user.
}
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax
}


From daemon Tue Mar 5 08:39:40 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 05 Mar 2002 09:33:22 -0500
Subject: New England Society for Microscopy

Contents Retrieved from Microscopy Listserver Archives
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This is a reminder of the New England Society for Microscopy evening
meeting to be held at the Lexington Laboratories of Raytheon Corp., in
Lexington, MA, on Tuesday March 12th. 2002, buffet supper at 6.00 p.m.,
scientific session 7:15 p.m.

Speakers will be:

John Thornton (Veeco Metrology Group)
"Scanned Probe Microscopies: Applications and Innovations"

and

Eric Hudson (MIT Dept. of Physics)
"Scanning Tunneling Microscopy: A Tool for Atomic Scale Measurement and
Manipulation"

Abstracts and full meeting information, including travel directions, and
other information about the society are available on the NESM Web Pages, at
http://www.msa.microscopy.com/MSALAS/NESM/NESMHome.htm

All interested people are invited and will be welcome to
attend. Preregistration is encouraged but not required.

Tony Garratt-Reed
President-elect




** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Tue Mar 5 08:54:29 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 5 Mar 2002 08:53:28 -0600
Subject: Re: Spiffy Freeware for H & E imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If I may chime in with a "me too". We also use Irfanview, and I
recommend to our users who have PCs. There's a similar (but more
developed and so more capable) program for Macs, "Graphic Converter"
( http://www.lemkesoft.com ). This one is shareware, $35, I think,
which is cheap for what it does. GC also converts many proprietary
image formats to standard formats, such as Bio-Rad pict or Gatan
Digital Micrograph to whatever is desired. We use and recommend both.

Phil

} I also find Irfanview very useful, and use it as my default image
} viewer. It is small and fast with a good number of facilities. Batch
} conversion (and / or file renaming) from one format to another is
} especially efficient. It displays, manipulates and saves a large
} variety of image formats. Setting up a slideshow is quick.
} Really useful software.
} The freeware version is for home use, the author requests a
} registration fee of 10 US dollars for non-personal use.
}
} (No connection with the author or program, just a satisfied user).
}
}
} Jan Coetzee
} Lab for Microscopy and Microanalysis
} University of Pretoria, South Africa.
} Tel: 012-420-2075, Fax 012-362-5150
} www.up.ac.za/academic/electron/emunit1.htm
}
}
}
}
} Paula Sicurello wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hi Listers,
} }
} } I just found out about a spiffy new freeware called IrfanView
} } (pronounced earfan-view).
} }
} } We were having trouble capturing H&E colors using PhotoShop. I
} } then heard from the guy who does tech support for the RGB camera
} } that we use about IrfanView. It is GREAT! We capture our images
} } and the colors are pretty true.
} }
} } We haven't figured it all out yet, so we still use PhotoShop for
} } other stuff, but I recommend that you take a look at the program
} }
} } Go to www.irfanview.com
} }
} } Has anybody else used this software? What's your opinion?
} }
} } Paula :-)
} }
} } p.s. I have no connection to this product I'm just a happy user.
} }
} } Paula Sicurello
} } George Washington Univ. Medical Center
} } Dept. of Pathology, Ross Hall rm 505
} } Electron Microscope Lab
} } 2300 Eye St.
} } Washington, DC 20037
} } 202-994-2930 phone
} } 202-994-2518 fax

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Tue Mar 5 10:14:40 2002



From: Jim Quinn :      jquinn-at-www.matscieng.sunysb.edu
Date: Tue, 5 Mar 2002 11:01:14 -0500
Subject: re: IrfanView (spiffy)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Paula and others -

IrfanView is great software. However, it is not new.
It dates back to 1996, with continual upgrades. The
current version is 3.61. You can get it from the IrfanView
site or WinSite. WinSite is usually quicker. The "zip"
file will fit on a floppy, it is amazingly small and powerful.

At the bottom of this response is the "about" file, which
contains the supported file types.

JQuinn

PS: ........also no connection to Irfan, just a happy user!

} From Microscopy-request-at-sparc5.microscopy.com Tue Mar 5 02:52:24 2002
} Date: Mon, 04 Mar 2002 15:27:49 -0500
} From: "Paula Sicurello" {patpxs-at-gwumc.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Subject: Spiffy Freeware for H & E imaging
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Listers,
}
} I just found out about a spiffy new freeware called IrfanView (pronounced earfan-view).
}
} We were having trouble capturing H&E colors using PhotoShop. I then heard from the
} guy who does tech support for the RGB camera that we use about IrfanView. It is GREAT!
} We capture our images and the colors are pretty true.
}
} We haven't figured it all out yet, so we still use PhotoShop for other stuff, but
} I recommend that you take a look at the program
}
} Go to www.irfanview.com
}
} Has anybody else used this software? What's your opinion?
}
}
} Paula :-)
}
} p.s. I have no connection to this product I'm just a happy user.
}
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax
}
}
-------------------------------------------------------------------------------
File : 'about.txt' - Info about IrfanView
Author: Irfan Skiljan
E-Mail: irfan-at-linux.tuwien.ac.at
WWW : http://www.irfanview.com
-------------------------------------------------------------------------------

What is IrfanView ?

IrfanView is a fast FREEWARE image viewer/converter for Win9x/NT, Windows 2000
and Windows XP.

Supported file formats:
AIF, ANI/CUR, ASF, AU/SND, AVI, BMP/DIB, CAM (Casio JPG), CLP, Dicom/ACR,
DJVU, EMF/WMF, EPS, FlashPix (FPX), FSH, G3, GIF, ICO/ICL/EXE/DLL, IFF/LBM,
IMG (GEM), JPG2000, JPG/JPEG, KDC, LDF, LWF, MED, MID/RMI, MOV, MP3, MPG/MPEG,
NLM/NOL/NGG, PBM/PGM/PPM, PCX/DCX, PhotoCD, PNG, PSD, PSP, RAS/SUN,
RealAudio (RA), RLE, SFF, SFW, SGI/RGB, SWF (Flash/Shockwave), TGA,
TIF/TIFF, WAV, WBMP, XBM, XPM.

Support for Apple QuickTime: allows IrfanView to read following
formats: MOV, QTIF, Mac PICT, FLI/FLC.

Microsoft Media Player PlugIn: allows IrfanView to read following
formats: ASF, AU/SND/AIF, AVI, DAT (VideoCD), MID/RMI, MOV, MP3,
MPG/MPEG, WAV, WMA, WMF, etc.

Some features of IrfanView:
Multi language support, Thumbnail option, preview option, slideshow,
drag & drop support, fast directory view (fast moving through directory),
batch conversion, email option, audio CD player, print option,
change color depth, scan support, cut/crop, effects (sharpen, blur,
Photoshop filter factory), capturing, extract icons from EXE/DLLs,
lossless JPG rotation, many hotkeys, many command line options ...

IrfanView was the first Windows graphic viewer (worldwide) with Animated-GIF
support !

FREEWARE for non commercial use !

Enjoy ! :-)

-------------------------------------------------------------------------------
IrfanView software is provided "as-is".
No warranty of any kind is expressed or implied.
-------------------------------------------------------------------------------



From daemon Tue Mar 5 10:50:13 2002



From: paques-at-nizo.nl
Date: Tue, 5 Mar 2002 17:43:19 +0100
Subject: short course announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Short Course
?Specific localisation methods and microscopy in Food Research.?

Sunday May 5th, 2002, 8:00 am - 6:00 PM
Electron Microscopy Centre, McGill University
Montréal, Québec, Canada

Sponsored by the Food Structure & Functionality Forum Division of the AOCS

Short Course Organizer:
Marcel Paques, Wageningen Centre for Food Sciences / Unilever R&D Vlaardingen,
The Netherlands

Spreadability, shelve life, fracture behaviour, and creaminess are examples of
functional properties of food products. These properties originate from the
microscopic structure of products. Specific localisation techniques and
microscopy are powerful tools to facilitate intelligent modification of
ingredient composition or processing to obtain targeted food product properties.
The short course is aimed at R&D personnel in the Foods area (fundamental
research, innovation, and product development). The course consists of lectures
and an intensive hands-on practical section providing participants with
sufficient basic knowledge and skills to set-up and implement the methods in
their own work. Registered participants are encouraged to submit
application-related questions to the instructors by email prior to the short.
In addition a personal consultation is offered to each participant scheduled (by
appointment) during the AOCS Annual Symposium 2002 in the days directly
following the short course. Contact Marcel Paques at paques-at-nizo.nl with
questions.


Programme topics include:

· Pre-course consultation (by email) with participants to ensure the course
content is relevant and applicable to participants?
interests
· Introduction to specific localisation methods and principles
· Localisation strategies, marking options, and imaging approaches
· Experimental set-up, preparation and incubation procedures
· Demonstration examples
· Hands-on practical sessions
· Tailored help and advice during private consultation session following
short course

Course contributors:
Jan Leunissen (Aurion: immuno Gold Reagents & accessories, custom labeling,
The Netherlands)
Hong Yi (Emory Neurology Microscopy Core Laboratory, Emory University
Department of Neurology, USA)
Marcel Paques (Wageningen Centre for Food Sciences/Unilever Research
Vlaardingen, The Netherlands)
Registration Fee: $375. The registration fee includes complete course materials,
continental breakfast, lunch, two refreshment breaks, and transportation to and
from McGill University.

Space is limited so register online today!
http://www.aocs.org/meetings/am2002/fscourse.htm

This electronic message is sent by NIZO food research to its business partner
and may contain confidential information only to be used by the client. The
contents may not be used by, copied or revealed to any other person than the
addressee.
In case this message was mistakenly addressed to you, please return the message
to info-at-nizo.nl or call +31 (0)318 659 511




From daemon Tue Mar 5 11:32:34 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 05 Mar 2002 09:25:52 -0800
Subject: Re: Ask-A-Microscopist: Hitachi S 4500 SEM Question

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Ding,
If you have the instruction book that came with the S-4500, it should list
the X and Y scan times for each of the scanning and photo speeds. The nature
of the imaging of the SEM means that the scanning of the view or photo CRT
and the scanning of the electron beam on the sample surface must be the
same. The output signal of the secondary electron detector is continuous,
modulated by the number of secondary electron that strike it, so the SEM's
scan generator generates the raster of the electron beam on the sample
surface and puts the secondary electron signal where it belongs on the
viewing CRT.
I hope this answers your question.
At 06:02 PM 3/4/02 -0600, you wrote:
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (w-ding-at-northwestern.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} March 4, 2002 at 17:17:11
} ---------------------------------------------------------------------------
}
} Email: w-ding-at-northwestern.edu
} Name: Weiqiang Ding
}
} Organization: Northwestern univ
}
} Education: Graduate College
}
} Location: Evanston,IL, US
}
} Question: I am a user of Hitachi S 4500 SEM.
} I want to know the relationship of the second electron detector
} output signal and CRT display signal. As you know, the SEM has
} different scan mode,including TV mode, and more slow modes. I want to
} know the time for each line scan on CRT and also for whole frame
} corresponding to the electron beam scan on sample. Is there any
} relationship or simple equations for calculation.
}
} Thanks.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Tue Mar 5 11:53:25 2002



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Tue, 05 Mar 2002 09:45:33 -0800
Subject: mistake in z-axis info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In discussing the location of the z-axis information yesterday
I made a mistake, confusing a figure number for a note number. The
Z-step size is the last (15th) value in the first line of the notes.
the Z-start and z-stop are the 1st and 2nd values in the second note,
respectively.

This info is presented in Image/J with the Edit/Info command after
using the Bio-Rad plug-in.

Sorry,
Glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************


From daemon Tue Mar 5 15:01:16 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Tue, 5 Mar 2002 15:46:05 -0800
Subject: vibratome repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
I need to find someone who can fix an Oxford vibratome Model G.
The motor appears to be running but the cutting motion isn't happening.
Any suggestions for service would be greatly appreciated.
thanks,
Beth Richardson

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Tue Mar 5 15:23:10 2002



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Wed, 6 Mar 2002 10:17:06 +1200
Subject: ESEM with tensile stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

I am looking at the possibilities of using an environmental SEM
equipped with a tensile stage to look at plant tissue and foodstuffs.
At present none of the ESEMs in Australasia appear to have such
a stage.

I would appreciate information on instruments elsewhere that we
could use or alternatively the cost, and any technical difficulties, in
the purchase or construction of a tensile stage to fit into an
existing ESEM.

Currently I anticipate we will need access to a system in around 12
to 18 months.


Best

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From daemon Tue Mar 5 17:58:12 2002



From: Colin.Veitch-at-csiro.au
Date: Wed, 6 Mar 2002 10:51:49 +1100
Subject: Confocal/SNOM STIL Micromeasure system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day,

A colleague is considering purchasing a STIL (Sciences et Techniques
Industrielle de la Lumiere) Micromeasure Multi Axes Measuring System and has
asked for any opinions on the device.

It seems to be a cross between a confocal and SNOM system but doesn't have
the resolution of either. The lower resolution is not an issue.

If anyone has had experience and or knowledge of this device, I (and my
colleague) would appreciate some input.

Thank you in advance.

Colin Veitch

Instrumentation Scientist
Late Stage Innovation Group
CSIRO Textile and Fibre Technology
PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au
Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Fax: +61 (0) 3 5246 4811


The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may not
copy, distribute or take any action in reliance on it. If you have received
this message in error, please telephone CSIRO Textile and Fibre Technology
on +61 3 5246 4000.



From daemon Tue Mar 5 18:06:45 2002



From: Roberto C :      robertoc-at-fire2wire.com
Date: Tue, 5 Mar 2002 16:00:59 -0800
Subject: RE: Ask-A-Microscopist:How to observe Cellular Mitosis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey guys, Greetings!
Just looking through the discussions and although I am not too up on
this discussion, I would like to invite you to view the QPm product by
IATIA. It permits digital imaging through a 10 bit camera and bright
field microscope and creates intensity free quantitative phase imaging,
as well as virtual modalities of DF, DIC, Hoffman and Zerniky Phase
contrast.
This technique allows stain free and contrast enhances BF imaging and
then the creation of DIC, and Phase contrast, etc.. You can measure much
more accurately because of the exclusion of the halo effect on the
fringes of the sample architecture. Also great for imaging facility
since you can take an image and send through the net to a collogue or
group of students to view and analyze. We call it a digital slide.
Can explain more later if you wish.
Thanks and best regards,

Roberto Casillas
General Manager, Americas Region
Iatia Group
P.O. Box 1087
Salida, CA 95368
United States
Tel (209) 545-4483
Fax (209) 545-4518
Mobile (209) 614-4135
Email rcasillas-at-iatia.com.au
Website www.iatia.com.au
This message and any files transmitted with it are confidential and are
intended solely for the use of those persons to whom the message is
addressed. If you have received this message in error, please destroy
and delete this message from your computer. Any unauthorised form of
reproduction of this message or any files transmitted with it is
strictly prohibited. Iatia Group does not make any warranty concerning
the accuracy of or security of any information electronically
transmitted and disclaims all liability for the accuracy of or proper
and complete transmission of any information contained or purportedly
contained in this message, and for any delay in its receipt. If you have
received this message in error, please
notify: rcasillas-at-iatia.com.au



-----Original Message-----
} From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
Sent: Monday, March 04, 2002 9:58 AM
To: 'dan-at-isaacson.net'
Cc: 'List-Microscopy'


Hi Dan,

Differential Interference Contrast (DIC)! or barring that, phase
coupled
with timed-sequence photography or just a plain video camera. Normal
cells
get thru the process in under 30min.

Get on the net and query Google with the following: "mitosis university
of
washington".

Look what I found in just a minute:

http://www.science.smith.edu/departments/Biology/Bio112/mitosislab.html

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


} ----------
} From: dan-at-isaacson.net
} Sent: Saturday, March 2, 2002 10:42 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist:How to observe Cellular Mitosis?
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (dan-at-isaacson.net) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
} March 1, 2002 at 17:27:04
}
------------------------------------------------------------------------
--
} -
}
} Email: dan-at-isaacson.net
} Name: Dan Isaacson
}
} Organization: Seattle Central Community College
}
} Education: Undergraduate College
}
} Location: Seattle, Washington
}
} Question: I live in Seattle and I was wondering what would be the
} best place/microscope/technique to observe cellular mitosis. More
} specifically I'm looking to observe the mitoic spindles through
} interphase, prophase, prometaphase, metaphase, anaphase, and
} telophase. Considering the size of spindles and the microtubule
} fibers that connect to the chromosomes it may be very difficult to
} observe. But any help you can give me would be greatly appriciated.
}
} Thanks,
} Dan Isaacson
}
}
------------------------------------------------------------------------
--
} -
}
}



From daemon Tue Mar 5 18:44:16 2002



From: Shane Roberts :      roberts-at-southbaytech.com
Date: Tue, 05 Mar 2002 16:32:56 -0800
Subject: Re: TEM Sample out of the eutectic Ceramic oxide NiO/ZrO2

Contents Retrieved from Microscopy Listserver Archives
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Dear Nasim:

Silicon carbide papers generally are not very good at handling brittle
materials that tend to crack during fine grinding
and polishing. The SiC papers are typically made of a thick paper
substrate that is irregular in shape with small
undulations, even at the fine grit sizes. These tend to cause cracking
of small, thin samples as you might have seen.

To reduce this type of cracking the use of abrasive films (plastic
sheets with abrasive embedded in the surface) can
help eliminate this effect and will give you a smoother, more uniform
surface. This is especially critical in TEM
applications where you are trying to thin the sample down. I would
suggest using SiC abrasive films (plain backed)
on a glass plate where you should see a marked improvement in the
cracking problem you are experiencing.
Polishing on a cloth (Nylon or Rayon) with 1 micron aluminum oxide after
you thin the sample should give you a nice
surface to finish the sample with, followed by whatever technique you
are using. I might also suggest using a Tripod
PolisherTM tool where you have close control of the load applied to the
sample during the polishing process, this
could also greatly improve your results.

I hope this helps and good luck.

Best Regards,

Shane Roberts
South Bay Technology, Inc.


Note: South Bay Technology is a manufacturer of the Tripod PolisherTM
and is a supplier of consumables.



From daemon Tue Mar 5 18:57:46 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Tue, 05 Mar 2002 17:18:14 -0500
Subject: Re: Protein dimers and chains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Take a look at reticle.com (Klarmann Rulings. Inc)

It is a good source of information, even if they may not have the cover
slips you need...


----- Original Message -----
} From: "Peter Van Osta" {pvosta-at-unionbio-eu.com}
To: "MSA" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, March 05, 2002 1:23 AM


on 2/4/02 5:21 PM, Jensen, Karen at Karen_Jensen-at-urmc.rochester.edu wrote:
}
} Dear Listers:
}
} I have been trying to image with negative stain, protein chains and protein
} dimers with 2.0% Uranyl Acetate. It seems when I pipet onto the grid,
} various dilutions (10-100x) of the buffered protein dimer sample, I get alot
} of protein chain formation. I want the dimer form to stay in that
} configuration-- not link up into chains. I am photographing at 100,000
} using 75 kv on a traditional Hitachi 7100 TEM.
}
} Does anyone out there work with these types of specimen? Is there a special
} way to prep the grid, dry the grid, etc.? Should I use some other type of
} grid besides the formvar/carbon coated copper grid? Should I sonicate the
} specimen before I place the sample on the grid?
}
} Thanks for any help you can provide.
}
Dear Karen,
UO2 is very acid, so maybe the buffering capacity of your sample is
being overwhelmed, and the resulting low pH causes the chain formation. In
that case, try a negative stain with a more neutral pH, such as NH4MoO4.
The carbon on your grids may be hydrophyllic or hydrophobic depending on how
fresh it is and whether it has been glow-discharged before use. This can
greatly affect the behavior of the protein in a way which varies for
different proteins, so must be determined for each case. Sonication could
also do either harm or good depending on the properties of the individual
protein (of course, heating due to the sonication will [almost] always be
harmful). The answers to the questions in your second paragraph are all
"yes". That is, special preparation may be required to get the appropriate
value for a critical parameter so your protein remains dimer; try other
types of grid, sonication, etc., to see the effects of varying parameters
such as surface, pH, etc.. Cryo-preparation, followed by cryosubstitution
or lyophyllization, might also be advantageous. Good luck.
Yours,
Bill Tivol



From daemon Tue Mar 5 22:42:33 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 05 Mar 2002 21:35:48 -0800
Subject: Re: Protein dimers and chains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi JQuinn:

Where can I download this free software for non-comnercial use?

Regards,

Xianglin Li

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411


----- Original Message -----
} From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}


Dear Karen,

Proteins are 'biochemical samples' and should be treated accordingly:
- what is the protein concentration?
-what ionic conditions for this protein and how you know that it's optimal
for dimerization?
- how you know that the chains formed during negative staining procedure?
- and so on...

In general, protein's oligomerization depends from ionic conditions and
concentration. You, probably better to use gel-filtration to see in which
form your protein are. As soon as you know conditions for protein,
determine optimal dilution for EM. I would recommend to use
Valentine-technique: adsorb protein on the carbon film and then move it to
the drop with staining solution. 2% UA is to much I think. 1% is very
standard for proteins. You may try freshly prepared uranyl formiate if
protein could not survive in UA. Mixing protein with staining solution is
very bad idea I think. Good luck, Sergey

At 02:18 PM 3/5/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Mar 6 02:42:11 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Wed, 06 Mar 2002 09:32:53 +0100
Subject: Re: Ask-A-Microscopist:How to observe Cellular Mitosis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have seen a product from the company "Cambridge Research &
Instruments" (CRI) to visualize microtubuli. I believe it is called
"spindleview" which enables viewing mitotic spindles, microtubuli, etc.
You can have a look at their website:

http://www.cri-inc.com/

Best regards,

Peter

P.S. I have no commercial relation with CRI.

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

======================================================
"Monson, Frederick C." wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Dan,
}
} Differential Interference Contrast (DIC)! or barring that, phase coupled
} with timed-sequence photography or just a plain video camera. Normal cells
} get thru the process in under 30min.
}
} Get on the net and query Google with the following: "mitosis university of
} washington".
}
} Look what I found in just a minute:
}
} http://www.science.smith.edu/departments/Biology/Bio112/mitosislab.html
}
} Regards,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} West Chester University
} West Chester, Pennsylvania, USA, 19383
} 610-738-0437
} fmonson-at-wcupa.edu


From daemon Wed Mar 6 06:04:31 2002



From: Tony Bruton :      Bruton-at-nu.ac.za
Date: Wed, 06 Mar 2002 13:55:44 +0200
Subject: Re: ESEM with tensile stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ian

In April last year I saw an ESEM at a research lab in Denmark that was
equipped with a tensile stage, they were looking at wood. The system
worked well in demos of wood fractures, I believe that they constructed
it themselves.

I don't remember the name of the lab but our host for the User Group
meeting, Lief Hoslet Christensen at Leif.H.Christensen-at-teknologisk.dk ,
will be able to tell you where he took us when we went walkabout during
the meeting.

Regards

Tony

Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website: www.nu.ac.za/microscopy.asp
Email: bruton-at-nu.ac.za
Postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa

} } } "IAN HALLETT" {ihallett-at-hortresearch.co.nz} 03/06/02 12:17AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America


Dear All

I am looking at the possibilities of using an environmental SEM
equipped with a tensile stage to look at plant tissue and foodstuffs.
At present none of the ESEMs in Australasia appear to have such
a stage.

I would appreciate information on instruments elsewhere that we
could use or alternatively the cost, and any technical difficulties, in

the purchase or construction of a tensile stage to fit into an
existing ESEM.

Currently I anticipate we will need access to a system in around 12
to 18 months.


Best

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify

the sender and delete all material pertaining to this e-mail.
______________________________________________________




From daemon Wed Mar 6 08:23:15 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 6 Mar 2002 09:18:25 -0500
Subject: Re: Ask-A-Microscopist: Hitachi S 4500 SEM Question

Contents Retrieved from Microscopy Listserver Archives
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Dr. Ding;

There is a "Photo Conditions" set-up screen on the 4500 that will give you
these conditions. Top row of buttons.

Peter Tomic
Anadigics

-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Tuesday, March 05, 2002 12:26 PM
To: w-ding-at-northwestern.edu
Cc: Microscopy-at-sparc5.microscopy.com


Dear Dr. Ding,
If you have the instruction book that came with the S-4500, it should list
the X and Y scan times for each of the scanning and photo speeds. The nature
of the imaging of the SEM means that the scanning of the view or photo CRT
and the scanning of the electron beam on the sample surface must be the
same. The output signal of the secondary electron detector is continuous,
modulated by the number of secondary electron that strike it, so the SEM's
scan generator generates the raster of the electron beam on the sample
surface and puts the secondary electron signal where it belongs on the
viewing CRT.
I hope this answers your question.
At 06:02 PM 3/4/02 -0600, you wrote:
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (w-ding-at-northwestern.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} March 4, 2002 at 17:17:11
} ---------------------------------------------------------------------------
}
} Email: w-ding-at-northwestern.edu
} Name: Weiqiang Ding
}
} Organization: Northwestern univ
}
} Education: Graduate College
}
} Location: Evanston,IL, US
}
} Question: I am a user of Hitachi S 4500 SEM.
} I want to know the relationship of the second electron detector
} output signal and CRT display signal. As you know, the SEM has
} different scan mode,including TV mode, and more slow modes. I want to
} know the time for each line scan on CRT and also for whole frame
} corresponding to the electron beam scan on sample. Is there any
} relationship or simple equations for calculation.
}
} Thanks.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Wed Mar 6 11:08:48 2002



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 6 Mar 2002 12:00:02 -0500 (EST)
Subject: EM Tech job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a mid-level EM tech position open. The title is EM Tech, Senior.

Our laboratory is responsible for all the electron microscopy
surgical pathology and diagnostic virology by EM for Duke Hospital. We
have 5 techs who share the duties which include tissue processing and thin
sectioning as well as fluid specimen preparation (e.g., cerebrospinal
fluid, stool, urine) for the identification of viruses. We process over
1200 clinical specimens per year and do a modest amount of research
microscopy. The new person would need to be proficient in electron
microscopy and ultramicrotomy but could learn virus identification and
tissue morphology on the job. If you are interested, please contact me
off line for details.

Sara Miller




Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265






From daemon Wed Mar 6 11:58:59 2002



From: Xianglin Li :      Xianglin_Li-at-student.uml.edu
Date: Wed, 06 Mar 2002 12:52:49 -0500
Subject: Need Help for Peak Dissolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all,

I am doing some work of XPS data interpretation. I met a problem that
some peaks were overlapped there. I want to separate them and find out
the information I need for each of them.

Somebody recommend me to use software DTSA, which could be used in SEM
EDX analysis to dissolve the peaks. But I don¡¯t know how to import a
XPS spectrum into DTSA.

Or, if somebody has some good idea of how to separate two or more
overlapped peak, could you please share with me?

Thank you in advance!

Sincerely, Xianglin Li


Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Wed Mar 6 12:09:01 2002



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Wed, 06 Mar 2002 10:01:59 -0800
Subject: Re: Cover slips with grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:59 PM 03/04/2002 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Mar 6 13:30:20 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 06 Mar 2002 10:34:12 -0600
Subject: Re: re: IrfanView (spiffy)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I tried the link that had been cited in the original post and it does
appear to be dead. There was another link listed for Winsite. Instead, I
tried a search for IrfanView and found several locations that offer it
besides the home page. One is given below. It eventually led me to
Tucows.com for the files.

http://www.ryansimmons.com/users/irfanview/

It will be nice to see the homepage back up, because I presume that it
would be the source of more information

Warren

At 11:34 PM 3/5/02 -0500, you wrote:

} Hi JQuinn:
}
} Where can I download this free software for non-comnercial use?
}
} Regards,
}
} Xianglin Li
}
} Center for Advanced Material
} Department of Chemical Engineering
} University of Massachusetts, Lowell
} Xianglin_Li-at-student.uml.edu
} Tel: 978-934-3411

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Wed Mar 6 14:29:27 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 06 Mar 2002 15:22:15 -0500
Subject: Mining for Gold Particles, Immuno style

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I'm back and digging for gold (information on pre-embed gold labelling that is) on adherent cells in culture. I have someone who wants to look for labelling in 3 areas, depending on the cell type (mutants, controls, knock-outs) all of which have a gfp worked into them. The label can be on either the plasma membrane surface, on the mitochodrial membrane surface, or floating around in the cytoplasm. What is the easiest and best way to pre-embed label for these babies?

I've done post embed labelling using LR White and the traditional ways of immuno, which we might try first. But I would like to know the best way to permeabilize the cells and maintain ultrastructure at the same time. I know there are nanoprobes and other tiny golds out there, I don't know if there are any pick your creature anti-gfp golds out there.

Any information you can pass my way will be greatly appreciated.

Mining for gold on the web,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Wed Mar 6 16:31:32 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 6 Mar 2002 19:09:50 -0400
Subject: Embedding cleared undecalcified bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
Does anyone have recommendation for embedding cleared /
stained* undecalcified bone stored in 100% glycerol in resin for LM
and EM imaging.
Rosemary

* alizarin red-bone + alcian blue (collagen)
--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Wed Mar 6 16:32:26 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 06 Mar 2002 17:26:57 -0500
Subject: need images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
Does anyone in North America have a FEG-SEM with cryostage who could look at a couple of hydrated samples for us in the next week? We have tungsten filament and cryo but it doesn't do the job.

Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Wed Mar 6 16:36:07 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Wed, 6 Mar 2002 17:29:06 -0800
Subject: vibratome service - thanks

Contents Retrieved from Microscopy Listserver Archives
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Content-Type: text/plain; charset="us-ascii"


Hi all,
Special thanks to those who replied to my question about vibratome repair
service. Thanks to y'all we have help.
best regards,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Wed Mar 6 16:37:07 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 6 Mar 2002 12:31:28 -1000 (HST)
Subject: Low level tech job at University of Hawaii

Contents Retrieved from Microscopy Listserver Archives
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Hi, All-

No, this is not your dream job unless you are planning to come live in
Honolulu anyway! It is a low-level (Research Associate II), half-time job
in the Biological EM Facility. We may have enough for full-time soon, and
for at least three years.

We are bascially looking for someone to help out the Facility
Supervisor/Senior Tech/the only tech/(me) in this core facility. We have a
LEO 912 EFTEM, a Hitachi S-800 FESEM, a Bio-Rad 1024 laser scanning
confocal microsocpe, and we are purchasing an upright fluorescence
compound microscope, a stereo zoom microscope, and a Magnafire SP digital
camera, plus PC and Mac imaging stations. We train users, perform all
tasks as a service, or any combination thereof.

Minimum qualifications include a BA or BS in biological science with
coursework in cell biology. Any experience with light or electron
microscopes is desireable, and I really would like to find someone with
fluorescence experience.

I can supply the complete duties and qualifications upon request.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Mar 6 16:49:32 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 6 Mar 2002 17:28:37 -0500
Subject: Need Help for Peak Dissolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can import files into DTSA in the EMSA format mode. You can find that info at the EMMPDL software library. You will have to put your data in ASCII mode and put in the appropriate headers. That would not be difficult. There is a software analysis program available for XPS analysis that is not too expensive. It can be found at the following web site: http://www.xpsdata.com/ and is called "Spectral Data Processor".

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Xianglin Li [mailto:Xianglin_Li-at-student.uml.edu]
Sent: Wednesday, March 06, 2002 12:53 PM
To: Microscopy-at-sparc5.microscopy.com


Hi, all,

I am doing some work of XPS data interpretation. I met a problem that
some peaks were overlapped there. I want to separate them and find out
the information I need for each of them.

Somebody recommend me to use software DTSA, which could be used in SEM
EDX analysis to dissolve the peaks. But I don¡¯t know how to import a
XPS spectrum into DTSA.

Or, if somebody has some good idea of how to separate two or more
overlapped peak, could you please share with me?

Thank you in advance!

Sincerely, Xianglin Li


Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Wed Mar 6 21:10:41 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 06 Mar 2002 20:57:07 -0600
Subject: Re: IrfanView (spiffy)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Straszheim: the www.irfanview.com link worked for me (using Netscape
Communicator 4.72). The site listed in your message is a mirror for the
IrfanView site and looks and works just like the original. So you're not missing
any information by using the link you found.

Warren E Straszheim wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I tried the link that had been cited in the original post and it does
} appear to be dead. There was another link listed for Winsite. Instead, I
} tried a search for IrfanView and found several locations that offer it
} besides the home page. One is given below. It eventually led me to
} Tucows.com for the files.
}
} http://www.ryansimmons.com/users/irfanview/
}
} It will be nice to see the homepage back up, because I presume that it
} would be the source of more information
}
} Warren
}
} At 11:34 PM 3/5/02 -0500, you wrote:
}
} } Hi JQuinn:
} }
} } Where can I download this free software for non-comnercial use?
} }
} } Regards,
} }
} } Xianglin Li
} }
} } Center for Advanced Material
} } Department of Chemical Engineering
} } University of Massachusetts, Lowell
} } Xianglin_Li-at-student.uml.edu
} } Tel: 978-934-3411
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 23 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Mar 7 03:51:07 2002



From: Andy Horsewell :      horsewell-at-ipl.dtu.dk
Date: Thu, 07 Mar 2002 10:41:34 +0100
Subject: Re: ESEM with tensile stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopy subscribers,

Following the thread on ESEM with tensile stage, referred to by Tony Bruton

"In April last year I saw an ESEM at a research lab in Denmark that was
equipped with a tensile stage, they were looking at wood. The system
worked well in demos of wood fractures, I believe that they constructed
it themselves. I don't remember the name of the lab but..."

The name of the lab is Risoe National Laboratory, Department of Materials
Research. The tensile stage is indeed home built, in close collaboration
between myself and Alan Heaver of Cambridge Engineering Dept., UK. We built
the stage in 1995, and did indeed use it to look at tensile testing of wood
(post fatigue damage in mahogany windmill wings together with Clare Hacker
from the University of Bath). We also looked at bamboo and grasses, again
tensile testing (with Ulrike Wegst, Cambridge Engineering).

The rig is still being used for in-situ tensile testing of a variety of
materials that we do not want to carbon- or gold-coat because large
deformations or cracks cause local peeling of the coating. The latest
materials to be studied are high Tc superconducting tape (BSSCO in Ag) see:

A. Horsewell, B.F. Sørensen & P. Skov-Hansen Materials Congress 2002, IoM,
London, April 2002 "In-situ observation of crack formation in BSSCO tapes".

The original rig can also be use to produced 3-point bending and
indentation, see: O. Jørgensen & A. Horsewell (1997) Acta Mater., 45,
3431-3444 "On the indentation failure of carbon-epoxy crossply laminates,
and its suppression by elasto-plastic interleaves".

What's more, a new rig was designed by myself and B. F. Sørensen to do
controlled crack growth experiments in brittle ceramics, again in-situ in
the ESEM. See for example: Sørensen, B.F. and Horsewell. A., (2001) J. Am.
Ceram. Soc. 84 (9), 2051-2059 “Crack growth along interfaces in porous
ceramic layers”.

I left the Risoe lab. 3 years ago to return to university teaching, at DTU
Denmark, and still collaborate closely with Risoe. Current questions on the
Risoe ESEM should be addressed to Jørgen Bilde-Sørensen.

Hope this information is useful.
Regards,

Andy Horsewell
Technical University of Denmark
Materials & Process Technology
Building 204
DK-2800 Lyngby, Denmark



From daemon Thu Mar 7 06:29:12 2002



From: Alexander Black :      alexander.black-at-nuigalway.ie
Date: Thu, 07 Mar 2002 12:20:58 +0000
Subject: DiATOME knife sharpening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Anyone who can help me out here, could you please email me privately
at -
alexander.black-at-nuigalway.ie


I need to get two DiATOME ultramicrotomy, 45o 3mm knives sharpened, and
would like to get as many quotes regarding cost (and
time period) as possible, as they seem to vary. So, if you are in this
area of expertise, please let me know!

Thanks

Alexander Black
Department of Anatomy
National University of Ireland, Galway
Republic of Ireland



From daemon Thu Mar 7 07:12:49 2002



From: j.bilde-at-risoe.dk
Date: Thu, 7 Mar 2002 07:04:11 -0600
Subject: Re: ESEM with tensile stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian Hallett wrote:
I am looking at the possibilities of using an environmental SEM
equipped with a tensile stage to look at plant tissue and foodstuffs.
At present none of the ESEMs in Australasia appear to have such
a stage. I would appreciate information on instruments elsewhere that we
could use or alternatively the cost, and any technical difficulties, in
the purchase or construction of a tensile stage to fit into an
existing ESEM.

Tony Bruton answered:
In April last year I saw an ESEM at a research lab in Denmark that was
equipped with a tensile stage, they were looking at wood. The system
worked well in demos of wood fractures, I believe that they constructed
it themselves.

Hi Ian and Tony,
It was the Materials Research Department at Risoe National Laboratory in
Denmark that Tony visited last year. And yes, we do have a stage for in-situ
stress-strain experiments in tension, compression or bending. The stage was
developed and constructed in a collaboraton between our laboratory and the
Engineering Department at University of Cambridge. The guy who knows most
about this stage is out of town for the moment, but I will ask him to
contact Ian for details when he comes back.

Best regards,
Jorgen.


{:::} ¤ {:::} ¤ {:::} ¤ {:::} ¤ {:::} ¤ {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm


From daemon Thu Mar 7 09:17:15 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 7 Mar 2002 09:09:09 -0600
Subject: RE: ESEM with tensile stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I believe GATAN is selling tensile stages for various types
of microscopes, and I was told some of them have Peltier cooling
sells, so that they could be used in ESEM. But I have not
seen these stages at work.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: IAN HALLETT [mailto:ihallett-at-hortresearch.co.nz]
} Sent: Tuesday, March 05, 2002 4:17 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: ESEM with tensile stage
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear All
}
} I am looking at the possibilities of using an environmental SEM
} equipped with a tensile stage to look at plant tissue and foodstuffs.
} At present none of the ESEMs in Australasia appear to have such
} a stage.
}
} I would appreciate information on instruments elsewhere that we
} could use or alternatively the cost, and any technical
} difficulties, in
} the purchase or construction of a tensile stage to fit into an
} existing ESEM.
}
} Currently I anticipate we will need access to a system in around 12
} to 18 months.
}
}
} Best
}
} Ian
}
}
} Ian Hallett
} HortResearch
} Mt Albert Research Centre
} Private Bag 92 169
} Auckland, New Zealand
} Fax 64-9-815 4201
} Telephone 64-9-815 4200
} EMail ihallett-at-hortresearch.co.nz
}
}
} ______________________________________________________
} The contents of this e-mail are privileged and/or confidential to the
} named recipient and are not to be used by any other person and/or
} organisation. If you have received this e-mail in error,
} please notify
} the sender and delete all material pertaining to this e-mail.
} ______________________________________________________
}
}


From daemon Thu Mar 7 09:24:46 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Mar 2002 09:18:36 -0600
Subject: Immunogold Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Immunogold Workshop Announcement

The Electron Microscopy Core Facility at the University of Missouri is hosting a three-day workshop on immunogold techniques from May 13-15, 2002. Dr. Jan Luenissen from Aurion Immunogold Reagents & Accessories, an internationally known expert in the field, will be the instructor for the workshop. The workshop will include lectures, hands-on training, round table discussions, and presentations on applications. Also, participants of the workshop will be able to work on their own samples during the workshop. The workshop main curriculum is detailed below. If you are interested in attending or need more information about the workshop, please contact the workshop technical coordinator Hong Yi by phone (404-727-8692) or email (hyi-at-emory.edu).


MAIN CURRICULUM

The properties of gold particles and their protein conjugates.
Theories underlying immunogold labeling protocols.
Silver enhancement of gold particles
Immunogold labeling on a variety of sample preparations for LM.
Immunogold labeling for EM
a. Pre-embedding immunogold labeling using ultrasmall gold conjugates and silver enhancement.
b. Post-embedding immunogold labeling using conventional colloidal gold conjugates and ultrasmalll gold conjugates.
Pre- and post-embedding double immunogold labeling.
Background minimization in immunogold labeling
Signal amplification in immunogold labeling.

Thanks and we hope to see you in Columbia!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Thu Mar 7 09:36:17 2002



From: John Basgen :      basgen-at-maroon.tc.umn.edu
Date: Thu, 7 Mar 2002 09:30:38 -0600
Subject: Stereology Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A stereology course approved by the International Society for Stereology will be
held May 26-30, 2002 at Hawks Nest State Park, West Virginia, USA. The course
will provide a practical and theoretical introduction to recent advances in
stereolgy.

The price for full registration including course fee, materials, single-room
accommodation, and all meals is US$1200.

The instructors are: HJG Gundersen, B Bakkenberg, JR Nyengaard, Karsten Nielsen,
and Dallas Hyde.

See the website of the International Soceity for Stereology
(http://www.stereologysociety.org) for more information or contact Jens
Nyengaard (nyengaard-at-iekf.au.dk).


.





John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu



From daemon Thu Mar 7 09:44:35 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 7 Mar 2002 09:40:28 -0600
Subject: Re: re: IrfanView (spiffy)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I just tried my original URL:
http://stud1.tuwien.ac.at/~e9227474/menu.html
which worked, automatically linking to:
http://irfanview.tuwien.ac.at/menu.html
Also this URL workied:
http://www.irfanview.com/

Phil

} I tried the link that had been cited in the original post and it
} does appear to be dead. There was another link listed for Winsite.
} Instead, I tried a search for IrfanView and found several locations
} that offer it besides the home page. One is given below. It
} eventually led me to Tucows.com for the files.
}
} http://www.ryansimmons.com/users/irfanview/
}
} It will be nice to see the homepage back up, because I presume that
} it would be the source of more information
}
} Warren
}
} At 11:34 PM 3/5/02 -0500, you wrote:
}
} } Hi JQuinn:
} }
} } Where can I download this free software for non-comnercial use?
} }
} } Regards,
} }
} } Xianglin Li
} }
} } Center for Advanced Material
} } Department of Chemical Engineering
} } University of Massachusetts, Lowell
} } Xianglin_Li-at-student.uml.edu
} } Tel: 978-934-3411
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 23 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Thu Mar 7 10:15:10 2002



From: tuttle-at-cox.net
Date: Thu, 7 Mar 2002 09:08:42 -0700
Subject: Re: IrfanView (spiffy)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can't get www.irfanview.com to load either, but it looks like
http://irfanview.tuwien.ac.at/english.htm works and it's in the
author's home country (Austria). It has links to multiple download
sites.

Dave Harrison


On 6 Mar 2002 at 20:57, Becky Holdford wrote:

} Dr. Straszheim: the www.irfanview.com link worked for me (using Netscape
} Communicator 4.72). The site listed in your message is a mirror for the
} IrfanView site and looks and works just like the original. So you're not missing
} any information by using the link you found.

}
} Warren E Straszheim wrote:
} } I tried the link that had been cited in the original post and it does
} } appear to be dead. There was another link listed for Winsite. Instead, I
} } tried a search for IrfanView and found several locations that offer it
} } besides the home page. One is given below. It eventually led me to
} } Tucows.com for the files.



From daemon Thu Mar 7 10:17:59 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 07 Mar 2002 10:12:21 -0600
Subject: Re: IrfanView (spiffy)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to Becky and Phil for the replies. I was at least able to get
through with IE 5.5 this morning to the regular site. It is still alive,
but it was running slow. My guess is that the longer connection (to Europe)
along with increased traffic (lots of microscopists checking out the
program?) might lead to the slower (or broken) connection. I guess that is
why mirror sites are setup in the first place.

Now to get around to checking out the program for myself...

Warren

At 08:57 PM 3/6/02 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Mar 7 11:09:05 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 07 Mar 2002 11:02:28 -0600
Subject: need rceommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers: We need to be able to quantify the space *between*
Ni grains in a metal film. Would grain size analysis
software be able to handle this? Or would some other type of
image analysis program be more useful? If this is a silly
question, I apologize for my ignorance. I'm not familiar
with the capabilities of either type of software.

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Mar 7 11:28:43 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 07 Mar 2002 09:22:24 -0800
Subject: Re: ESEM with tensile stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ian,
We are just about to take delivery of a variable pressure SEM for tensile
testing and three-point bending of epoxy-fibre composites. In this case we
bought a VPSEM that fit the stage we built for this research four years ago.
This stage was not easy or inexpensive to build. However, I remember that
Deben of the UK (www.deben.co.uk) make a variety of tensile and bending
stages and other accessories to fit in SEM's. They might be a good place to
start.
At 10:17 AM 3/6/02 +1200, you wrote:

} Dear All
}
} I am looking at the possibilities of using an environmental SEM
} equipped with a tensile stage to look at plant tissue and foodstuffs.
} At present none of the ESEMs in Australasia appear to have such
} a stage.
}
} I would appreciate information on instruments elsewhere that we
} could use or alternatively the cost, and any technical difficulties, in
} the purchase or construction of a tensile stage to fit into an
} existing ESEM.
}
} Currently I anticipate we will need access to a system in around 12
} to 18 months.
}
}
} Best
}
} Ian
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Thu Mar 7 11:54:09 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 07 Mar 2002 11:47:58 -0600
Subject: Re: need recommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suppose it all depends on what you mean by "between". In my years of
image analysis, I have seen many definitions for terms we use in everyday
conversation, but without a clear idea of what we mean.

I could interpret your question as determining the nearest neighbor
distance. I could also see approaching the issue by measuring grain size. I
could also see estimating it by counting the number of grains in a given
area. In many cases, the three approaches could give similar results,
probably in the cases where there is a single mode to the size
distribution. However, I can imagine some situations where the results
would be quite different, for example, where smaller grains are found at
the boundaries of much larger grains.

I have a few ideas of what algorithms might be applied and how. But I would
be interested to hear what might be suggested by those who are directly
involved with grain size analysis.

Warren

At 11:02 AM 3/7/02 -0600, Becky Holdford wrote:

} Listers: We need to be able to quantify the space *between*
} Ni grains in a metal film. Would grain size analysis
} software be able to handle this? Or would some other type of
} image analysis program be more useful? If this is a silly
} question, I apologize for my ignorance. I'm not familiar
} with the capabilities of either type of software.
}
} --
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Becky Holdford (r-holdford-at-ti.com)
} 972-995-2360
} 972-598-1291 (pager)
} SC Packaging FA Development
} Texas Instruments, Inc.
} Dallas, TX
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Mar 7 12:13:56 2002



From: Schmitz, Robert :      rschmitz-at-uwsp.edu
Date: Thu, 7 Mar 2002 12:07:36 -0600
Subject: RE: Embedding cleared undecalcified bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Why do you want to look at a cleared and stained specimens with the electron microscope?

I don't think that these specimens can be prepared for EM and if they could, I don't think they could yield any useful information. Cleared and stained specimens are "cleared" by immersing them in a trypsin solution for several weeks and then by immersing them in a KOH solution. This process digests the soft tissue away so that the remnant muscle is transparent when immersed in glycerol. The purpose of this procedure is to be able to visualize the three dimensional relationships of the skeleton of small organisms such as fish, amphibians, and developing fetuses. Depending on the intensity of the treatment the specimens can easily fall apart and the individual bones while still demonstrating their shape are almost certainly decalcified. The KOH and trypsin should also destroy the ultrastructure of the bone and cartilage cells as well as significantly degrade the proteins in the matrix of these tissues. If the cellular structure of the tissues is destroyed, why would you want to look at specimen's prepared in this way with EM?

In my work on skeletal structure and ultrastructure in fishes I have fixed the tissue with a conventional Karnovsky fixative, decalcified with EDTA and post-fixed with osmium. I understand that some people think that citric acid is a better decalcifying agent, but I haven't seen a paper describing this technique yet. (it is also very important to remove all of the EDTA before post-fixing with osmium). Check out one of my papers J. Morph. 226:1-24 (1996) for further details

Bob
Robert J. Schmitz
Department of Biology
University of Wisconsin Stevens Point
Stevens Point, WI 54481
715-346-2420
Email: rschmitz-at-uwsp.edu
http://biology.uwsp.edu/faculty/RSchmitz/home.html

} ----------
} From: Rosemary Walsh
} Sent: Wednesday, March 6, 2002 5:09 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Embedding cleared undecalcified bone
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear listers,
} Does anyone have recommendation for embedding cleared /
} stained* undecalcified bone stored in 100% glycerol in resin for LM
} and EM imaging.
} Rosemary
}
} * alizarin red-bone + alcian blue (collagen)
} --
} Rosemary Walsh, Manager
} The Electron Microscope Facility for the Life Sciences,
} A Shared Technology Facility, The Life Sciences Consortium
} 1 South FrearLab
} Penn State University
} University Park, PA 16802
} (814) 865-0212
} rw9-at-psu.edu
} http://www.lsc.psu.edu/stf/em/home.html
}
}
}


From daemon Thu Mar 7 12:25:43 2002



From: april691-at-cranfield.ac.uk
Date: Thu, 7 Mar 2002 13:13:51 -0500
Subject: Hey Fred

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fred,


It was nice to talk to you today I will send the proposal tonight.



Thanks,
Heidi


From daemon Thu Mar 7 12:25:43 2002



From: april691-at-cranfield.ac.uk
Date: Thu, 7 Mar 2002 13:13:51 -0500
Subject: Hey Fred

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fred,


It was nice to talk to you today I will send the proposal tonight.



Thanks,
Heidi


From daemon Thu Mar 7 13:09:50 2002



From: Barwood, Henry L :      hbarwood-at-indiana.edu
Date: Thu, 7 Mar 2002 14:01:29 -0500
Subject: Cold Cathode CL unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking for a cold cathode luminescence (CL) stage for personal use.
Anyone have an obsolete, or presently unused Luminoscope or Technosyn unit
they would like to sell? Of course, if you have a real junker that you would
like to get rid of, let me know. I would be interested in rebuilding such an
item.

Henry Barwood



From daemon Thu Mar 7 14:37:37 2002



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Thu, 7 Mar 2002 15:29:28 -0500
Subject: RE: DiATOME knife sharpening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Always try and send diamond knives for repolishing back to the manufacturer,
since they made it originally by their polishing techniques which were
developed for the specific diamond orientation that they think is best, a
most important factor. It's not that the others will aways do a terrible
job (but might), so the one who made it will likely do the best job. It
might be a bit more expensive, but that money should be recouped through
longer acceptable sectioning behaviour.

Tom
} ----------
} From: Alexander Black
} Sent: Thursday, March 07, 2002 7:20 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: DiATOME knife sharpening
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} Anyone who can help me out here, could you please email me privately
} at -
} alexander.black-at-nuigalway.ie
}
}
} I need to get two DiATOME ultramicrotomy, 45o 3mm knives sharpened, and
} would like to get as many quotes regarding cost (and
} time period) as possible, as they seem to vary. So, if you are in this
} area of expertise, please let me know!
}
} Thanks
}
} Alexander Black
} Department of Anatomy
} National University of Ireland, Galway
} Republic of Ireland
}
}


From daemon Thu Mar 7 14:46:23 2002



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Thu, 7 Mar 2002 15:41:08 -0500
Subject: RE: need rceommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Unless you have some really bizarre form of Ni film, you are talking about
the interface between adjacent crystallites, called a grain boundary.
Unfortunately, the conventionally accepted width of grain boundaries in
metallic systems is of the order of 1 nm or less, too low even for a FE-SEM.
So, could one take very high mag TEM images and subject same to image
analysis? Not with much accuracy, as the boundary has to be exactly
parallel to the electron beam or else geometric (tilt) effects will make it
appear much wider.

It sounds as though you might have some form of very fine-grained Ni,
perhaps even nanocrystalline in scale, say 2-20 nm, where there has been
good evidence that the grain boundaries are wider, and thus constitute a
significant volume fraction of the material. I'm not aware of any that used
direct imaging and image analysis, because the above effect is even worse,
since the grains may overlap in even the thinnest of TEM specimens. Check
'grain boundaries/nanocrystalline' in a search to see what methodology they
used to estimate interface volume. Maybe projecting from atomic resolution
imaging?

Tom

Dr. Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada K1A 0G1

ph. 613-992-2310
FAX 613-992-8735

email: malis-at-nrcan.gc.ca
(currently on assignment as Science Advisor to DG/MTB, can be reached at
613-995-7358, same email)


} ----------
} From: Becky Holdford
} Sent: Thursday, March 07, 2002 12:02 PM
} To: Microscopy ListServer
} Subject: need rceommendations for measuring space between grains
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers: We need to be able to quantify the space *between*
} Ni grains in a metal film. Would grain size analysis
} software be able to handle this? Or would some other type of
} image analysis program be more useful? If this is a silly
} question, I apologize for my ignorance. I'm not familiar
} with the capabilities of either type of software.
}
} --
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Becky Holdford (r-holdford-at-ti.com)
} 972-995-2360
} 972-598-1291 (pager)
} SC Packaging FA Development
} Texas Instruments, Inc.
} Dallas, TX
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
}
}


From daemon Thu Mar 7 15:50:37 2002



From: Michael L. Boucher :      mboucher-at-tc.umn.edu
Date: Thu, 07 Mar 2002 15:46:45 -0600
Subject: Anyone have an old EDS detector/dewar for giveaway?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of our specialists is trying to build a cooled sample holder for an
accelerator. He thinks that an old EDS dewar complete with coldfinger might
be usable to make one. Anyone in the U.S. got an old one lying about? We
would reimburse you for shipping and it wouldn't have to be packed the same
way a working one would be shipped.
Let me know.
Thanks.
Mike
********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************








From daemon Thu Mar 7 16:48:13 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 07 Mar 2002 16:38:04 -0600
Subject: Re: need rceommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I do have a really bizarre Ni film. I guess it's not really a film, but a
electroless Ni deposited layer.
This layer has a columnar form when seen in cross-section. Sometimes the
plating process is not
good and there are gaps between these columns which are visible from the top
surface.
The boss wants to know if there is some way of quantifying the area of the gaps.

I should have spelled this out more in my original post. My ignorance is really
showing now.

"Malis, Tom" wrote:

} Unless you have some really bizarre form of Ni film, you are talking about
} the interface between adjacent crystallites, called a grain boundary.
} Unfortunately, the conventionally accepted width of grain boundaries in
} metallic systems is of the order of 1 nm or less, too low even for a FE-SEM.
} So, could one take very high mag TEM images and subject same to image
} analysis? Not with much accuracy, as the boundary has to be exactly
} parallel to the electron beam or else geometric (tilt) effects will make it
} appear much wider.
}
} It sounds as though you might have some form of very fine-grained Ni,
} perhaps even nanocrystalline in scale, say 2-20 nm, where there has been
} good evidence that the grain boundaries are wider, and thus constitute a
} significant volume fraction of the material. I'm not aware of any that used
} direct imaging and image analysis, because the above effect is even worse,
} since the grains may overlap in even the thinnest of TEM specimens. Check
} 'grain boundaries/nanocrystalline' in a search to see what methodology they
} used to estimate interface volume. Maybe projecting from atomic resolution
} imaging?
}
} Tom
}
} Dr. Tom Malis
} Group Leader - Characterization
} Materials Technology Laboratory
} Natural Resources Canada (Govt. of Canada)
} 568 Booth St., Ottawa, Canada K1A 0G1
}
} ph. 613-992-2310
} FAX 613-992-8735
}
} email: malis-at-nrcan.gc.ca
} (currently on assignment as Science Advisor to DG/MTB, can be reached at
} 613-995-7358, same email)
}
} } ----------
} } From: Becky Holdford
} } Sent: Thursday, March 07, 2002 12:02 PM
} } To: Microscopy ListServer
} } Subject: need rceommendations for measuring space between grains
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Listers: We need to be able to quantify the space *between*
} } Ni grains in a metal film. Would grain size analysis
} } software be able to handle this? Or would some other type of
} } image analysis program be more useful? If this is a silly
} } question, I apologize for my ignorance. I'm not familiar
} } with the capabilities of either type of software.
} }
} } --
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Becky Holdford (r-holdford-at-ti.com)
} } 972-995-2360
} } 972-598-1291 (pager)
} } SC Packaging FA Development
} } Texas Instruments, Inc.
} } Dallas, TX
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} }
} }
} }

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Mar 7 17:17:57 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 7 Mar 2002 18:11:04 -0500
Subject: Re: need rceommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I worked with a similar porous columnar structure once. If you can differentiate the "gaps", i.e. porosity in your films from the grains, then you can do a simple point count which would give you the area fraction of porosity when viewed from the top. That would be a quantitative measure of the quality of the films. If you do not know how to do the stereological measurements, visit John Russ' tutorial website, http://www.reindeergraphics.com/tutorial/chap7/global01.html
for the Image Processing Toolkit and Fovea Pro software. There are relatively simple statistical tests to determine how many areas you would have to sample. See his handbook which is also cited on the web site.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Becky Holdford [mailto:r-holdford-at-ti.com]
Sent: Thursday, March 07, 2002 5:38 PM
To: Malis, Tom
Cc: Microscopy ListServer


I do have a really bizarre Ni film. I guess it's not really a film, but a
electroless Ni deposited layer.
This layer has a columnar form when seen in cross-section. Sometimes the
plating process is not
good and there are gaps between these columns which are visible from the top
surface.
The boss wants to know if there is some way of quantifying the area of the gaps.

I should have spelled this out more in my original post. My ignorance is really
showing now.

"Malis, Tom" wrote:

} Unless you have some really bizarre form of Ni film, you are talking about
} the interface between adjacent crystallites, called a grain boundary.
} Unfortunately, the conventionally accepted width of grain boundaries in
} metallic systems is of the order of 1 nm or less, too low even for a FE-SEM.
} So, could one take very high mag TEM images and subject same to image
} analysis? Not with much accuracy, as the boundary has to be exactly
} parallel to the electron beam or else geometric (tilt) effects will make it
} appear much wider.
}
} It sounds as though you might have some form of very fine-grained Ni,
} perhaps even nanocrystalline in scale, say 2-20 nm, where there has been
} good evidence that the grain boundaries are wider, and thus constitute a
} significant volume fraction of the material. I'm not aware of any that used
} direct imaging and image analysis, because the above effect is even worse,
} since the grains may overlap in even the thinnest of TEM specimens. Check
} 'grain boundaries/nanocrystalline' in a search to see what methodology they
} used to estimate interface volume. Maybe projecting from atomic resolution
} imaging?
}
} Tom
}
} Dr. Tom Malis
} Group Leader - Characterization
} Materials Technology Laboratory
} Natural Resources Canada (Govt. of Canada)
} 568 Booth St., Ottawa, Canada K1A 0G1
}
} ph. 613-992-2310
} FAX 613-992-8735
}
} email: malis-at-nrcan.gc.ca
} (currently on assignment as Science Advisor to DG/MTB, can be reached at
} 613-995-7358, same email)
}
} } ----------
} } From: Becky Holdford
} } Sent: Thursday, March 07, 2002 12:02 PM
} } To: Microscopy ListServer
} } Subject: need rceommendations for measuring space between grains
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Listers: We need to be able to quantify the space *between*
} } Ni grains in a metal film. Would grain size analysis
} } software be able to handle this? Or would some other type of
} } image analysis program be more useful? If this is a silly
} } question, I apologize for my ignorance. I'm not familiar
} } with the capabilities of either type of software.
} }
} } --
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Becky Holdford (r-holdford-at-ti.com)
} } 972-995-2360
} } 972-598-1291 (pager)
} } SC Packaging FA Development
} } Texas Instruments, Inc.
} } Dallas, TX
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} }
} }
} }

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Mar 7 17:51:12 2002



From: Rotole, John A. :      John.Rotole-at-ispat.com
Date: Thu, 7 Mar 2002 17:43:15 -0600
Subject: Need Help for Peak Dissolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning,

There is a web guide of available surface analysis software at the
website of the UK ESCA Users Group at

http://www.uksaf.org/software.html


XPSPEAK 4.1 by Raymund Kwok is a free download and may help get you started.

Have fun,

John

John A. Rotole, Ph.D.
Project Engineer
Coatings & Surface Technology
Ispat Inland Product Research
3001 E. Columbus Drive
East Chicago, Indiana 46312
Tel: 219-399-6308
Fax: 219-399-6562


-----Original Message-----
} From: Xianglin Li [mailto:Xianglin_Li-at-student.uml.edu]
Sent: Wednesday, March 06, 2002 11:53 AM
To: Microscopy-at-sparc5.microscopy.com


Hi, all,

I am doing some work of XPS data interpretation. I met a problem that
some peaks were overlapped there. I want to separate them and find out
the information I need for each of them.

Somebody recommend me to use software DTSA, which could be used in SEM
EDX analysis to dissolve the peaks. But I don¡¯t know how to import a
XPS spectrum into DTSA.

Or, if somebody has some good idea of how to separate two or more
overlapped peak, could you please share with me?

Thank you in advance!

Sincerely, Xianglin Li


Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411


From daemon Thu Mar 7 17:51:16 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 07 Mar 2002 15:45:41 -0800
Subject: RE: DiATOME knife sharpening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree: as soon as you choose manufacturer, you, probably, should use
their re-sharpening service. I don't think you may save $$ negotiating
re-sharpening price, but you could ask them about exchange program when
they offer to you brand new knife at the price of re-sharpening in exchange
on your old one. The good things about this program, that you could change
the knife type: exchange your 45o on new 35o or even on cryo and so
on. It works for Diatome. Have no inmterest in Diatome, but happy user.
Sergey

At 12:29 PM 3/7/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Mar 7 17:53:30 2002



From: Rick Bizzoco :      rbizzoco-at-sunstroke.sdsu.edu
Date: Thu, 7 Mar 2002 15:54:45 -0800
Subject: need enlarger bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Howdy all

I am looking for GE bulb BEV for an Omega D5 microfilm point source.
It is out of production and calls to a variety of sources have proved
fruitless. If anyone can help please contact Dr. Rick Bizzoco at
(619) 594-5396 or rbizzoco-at-sunstroke.sdsu.edu

Thanks in advance


From daemon Thu Mar 7 18:14:05 2002



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 7 Mar 2002 16:05:32 -0800
Subject: Re-embedding thick sections for ultratome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What is your favorite method for treating glass slides for thick sections
such that they can then be re-embedded? We re-embed by standing a
polymerized BEEM block on top of the section with a drop of epoxy between
the two. Without treating the slide first, it is often difficult to remove
the newly joined section and block.

TIA

Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Thu Mar 7 18:26:50 2002



From: DrJohnRuss-at-aol.com
Date: Thu, 7 Mar 2002 19:20:24 EST
Subject: Re: need recommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 3/7/02 6:24:00 PM, walck-at-ppg.com writes:

} I worked with a similar porous columnar structure once. If you can
differentiate
} the "gaps", i.e. porosity in your films from the grains, then you can do
} a simple point count which would give you the area fraction of porosity
} when viewed from the top. That would be a quantitative measure of the
} quality of the films. If you do not know how to do the stereological
measurements,
} visit John Russ' tutorial website,
http://www.reindeergraphics.com/tutorial/chap7/global01.html
} for the Image Processing Toolkit and Fovea Pro software. There are
relatively
} simple statistical tests to determine how many areas you would have to
} sample. See his handbook which is also cited on the web site

Thanks for the plug, Scott. The biggest problem with structures like this is
not the quantification of the image, but the sample prep. If the
columns/grains/etc. are significantly different in hardness than the stuff in
the gaps (or worse, if there is nothing in the gaps), then in sample
preparation there tends to be some dragging of the material that changes the
dimensions, usually making the gaps narrower in appearance than they really
are. It is difficult to overcome this. Even ion beam milling can cause the
effect.

John Russ



From daemon Thu Mar 7 18:48:45 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 7 Mar 2002 19:42:56 -0500
Subject: RE: need recommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Your welcome.

If the structure that she has is similar to what I had, then the porosity between the columnar grains is about constant through the thickness of the deposited coating. We had a structure that you could see dark spaces between grains that appeared "star-like". We modified the chemistry of our films and the porosity closed up as the films got denser and you could see it in the SEM from the surface. No sample preparation was done. However, I never quantified the porosity from these images, but it could be observed qualitatively.

____
Thanks for the plug, Scott. The biggest problem with structures like this is
not the quantification of the image, but the sample prep. If the
columns/grains/etc. are significantly different in hardness than the stuff in
the gaps (or worse, if there is nothing in the gaps), then in sample
preparation there tends to be some dragging of the material that changes the
dimensions, usually making the gaps narrower in appearance than they really
are. It is difficult to overcome this. Even ion beam milling can cause the
effect.

John Russ


From daemon Thu Mar 7 20:59:49 2002



From: ResearchNetwork.com :      info-at-researchnetwork.com
Date: 8 Mar 2002 04:08:18 -0000
Subject: Research Network Job Site

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Hi Becky,

You could try the software "scion image" to see whether it works or not.

Xianglin

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411


----- Original Message -----
} From: Becky Holdford {r-holdford-at-ti.com}

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From daemon Fri Mar 8 03:59:34 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Fri, 08 Mar 2002 10:49:26 +0100
Subject: Re: need recommendations for measuring space between grains

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Hi,

I agree that it mught be necessary to define the question more precise,
but for measuring interdistances between "objects" in order to quantify
the space *between* Ni grains in a metal film, there is an article which
describes a method to analyze distances between neighbours.

The method can be applied to any field where "regular" patterns have to
be detected, as long as the directional distribution of neighbours may
be neglected.

J. M. Geusebroek, A. W. M. Smeulders, F. Cornelissen, and H. Geerts.
Segmentation of tissue architecture by distance graph matching.
Cytometry, 35(1):12-22, 1999.

Best regards,

Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

================================================================
Warren E Straszheim wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I suppose it all depends on what you mean by "between". In my years of
} image analysis, I have seen many definitions for terms we use in everyday
} conversation, but without a clear idea of what we mean.
}
} I could interpret your question as determining the nearest neighbor
} distance. I could also see approaching the issue by measuring grain size. I
} could also see estimating it by counting the number of grains in a given
} area. In many cases, the three approaches could give similar results,
} probably in the cases where there is a single mode to the size
} distribution. However, I can imagine some situations where the results
} would be quite different, for example, where smaller grains are found at
} the boundaries of much larger grains.
}
} I have a few ideas of what algorithms might be applied and how. But I would
} be interested to hear what might be suggested by those who are directly
} involved with grain size analysis.
}
} Warren


From daemon Fri Mar 8 05:45:17 2002



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 08 Mar 2002 11:47:04 +0000
Subject: Re: Re-embedding thick sections for ultratome

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Rick

a simpler way might be make up resin slides if you know in advance. You
should be able to buy a mould from one of the e.m. suppliers.

We got one from Agar Scientific UK a few years ago although we've never
had much need for it so I can't tell you how well it works.

Malcolm

PS My apologies to Nestor - I keep switching off my signature card to
send to the list. But I don't think it turns off if a message is already
composed so I keep getting bounced for attachments. I will try harder
..


Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel +44 (0)191 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk


From daemon Fri Mar 8 07:27:28 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 8 Mar 2002 13:20:03 +0000 (GMT Standard Time)
Subject: Re-embedding thick sections for ultratome

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I have read that liquid nitrogen can be used to separate
slides from blocks.

Dave


On Thu, 7 Mar 2002 16:05:32 -0800 Rick Harris
{raharris-at-ucdavis.edu} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} What is your favorite method for treating glass slides for thick sections
} such that they can then be re-embedded? We re-embed by standing a
} polymerized BEEM block on top of the section with a drop of epoxy between
} the two. Without treating the slide first, it is often difficult to remove
} the newly joined section and block.
}
} TIA
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} 530 754 7536 fax
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Mar 8 07:29:47 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Fri, 8 Mar 2002 09:53:59 -0330
Subject: ReindeerGame's mag plug-in

Contents Retrieved from Microscopy Listserver Archives
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Just a cautionary note, possibly only pertaining to digitally captured SEM
images. I just began using this plug-in, figuring it would be much easier
than accessing my spreadsheet values. I noticed out-of-the-gate, they were
in disagreement.

The problem became obvious when I noticed the TIFF's pixel/inch (dpi)
setting was incorrect. I believe the acquisition software failed to embed
any resolution at all, and Photoshop assumed 72dpi. This would be wrong for
this SEM, because the dpi setting implies a 14" wide image when the given
magnification is for a 4by5 image. Therefore it would be important to know
for what size image your SEM's mag is appropriate (is 4x5 still a standard
size?), and make that change before applying the plug-in.

Works great! ... but I would apply the plugin to a new layer (so you can
move the bar to a preferred location), or build your preferred type of
micron bar based on it.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From daemon Fri Mar 8 08:23:17 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 8 Mar 2002 08:17:15 -0600
Subject: RE: need rceommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
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It looks like your gaps are big ehough, so you could try
electroplating with Cu. After polishing out the Cu layer
you (may be) could measure a size of the Cu islands in Ni.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

}
} I do have a really bizarre Ni film. I guess it's not really
} a film, but a
} electroless Ni deposited layer.
} This layer has a columnar form when seen in cross-section.
} Sometimes the
} plating process is not
} good and there are gaps between these columns which are
} visible from the top
} surface.
} The boss wants to know if there is some way of quantifying
} the area of the gaps.
}
} I should have spelled this out more in my original post. My
} ignorance is really
} showing now.
}


From daemon Fri Mar 8 08:48:42 2002



From: Louis Kerr :      lkerr-at-mbl.edu
Date: Fri, 08 Mar 2002 10:38:31 -0500
Subject: Summer 2002 Bio Microscopy Tech Position

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Hi, Becky:

The electroless plated Ni usually contains impurities such as P (5-15%), or
even Teflon (~5-20%) for some purpose. If you could, TOF-SIMS is an
alternative method to try. TOF-SIMS can directly "see" the elemental
distribution on the very surface of sample with ~100nm resolution. Of
course, sample needs to be polished so that the surface film does not give
you wrong information. On SEM images, there are a lot of story on the
boundary between the dark and the bright area (no matter what sample) and it
depends on many many variables. I do not think you can exclude these
artifacts from SEM imaging and get really true distance on SEM image if your
goal is set on {100 nanometer level.

Thanks,


Zhiyu Wang

----- Original Message -----
} From: "Becky Holdford" {r-holdford-at-ti.com}
To: "Malis, Tom" {malis-at-nrcan.gc.ca}
Cc: "Microscopy ListServer" {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 07, 2002 10:38 PM


2002 MICROSCOPY TECHNICIAN SUMMER POSITION AVAILABLE

The Marine Biological Laboratory has a summer position available for 10
to 15 weeks (June, July, and August) for a microscopy oriented
technician. We would like to attract someone with some knowledge of
biological preparative techniques and experience in any of the
following: laser scanning
confocal microscopy, TEM, SEM, and/or LM.

The technician will assist in the Central Microscopy Facility. The
technician's duties will be to check out incoming investigators in the
usage of our equipment and then to supervise its continuing usage and to
perform contract work for investigators. This may include fixation,
embedding, sectioning, scope use, darkroom work, etc. The technician will
also provide routine maintenance.

This is a short term and scientifically rewarding position. Salary will be
in the $8 to $10/hour range. Housing may be available to rent through MBL.

For more information, including a more detailed position description,
please contact Louis Kerr at: MBL, 7 MBL Street, Woods Hole, MA 02543.
Telephone, 508-289-7273; or at Email: lkerr-at-mbl.edu.

Please apply to: Human Resources, MBL, 7 MBL Street,
Woods Hole, MA 02543. or resume-at-mbl.edu.

You can visit our web site for online employment information and general
information regarding the Marine Biological Laboratory - http://www.mbl.edu/

An Equal Opportunity/Affirmative Action Employer/ Non-smoking workplace.


From daemon Fri Mar 8 09:51:27 2002



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Fri, 8 Mar 2002 09:44:17 -0600
Subject: resharpening

Contents Retrieved from Microscopy Listserver Archives
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I agree with the postings that recommend using the same knife maker for
resharpening. I sent two Diatomes to be resharpened by another
manufacturer and when the knives were returned they didn't wet properly
and I had to send them back a 2nd time. I don't know if the problem has
been resolved since I don't have them back, but that was a least 4
months without them. Fortunately, I had other knives to use and they sent
a loaner to use in the meantime. Apparently, the two manufacturers use
different wetting processes.


Hank Adams
Core Manager
Microscopy Facility
Department of Molecular Genetics
MD Anderson Cancer Center
Houston, TX.



From daemon Fri Mar 8 09:57:33 2002



From: William Oxberry :      William_Oxberry-at-downstate.edu
Date: Fri, 8 Mar 2002 10:50:22 -0500
Subject: Re:need enlarger bulb

Contents Retrieved from Microscopy Listserver Archives
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Try www.bulbdirect.com



From daemon Fri Mar 8 10:16:30 2002



From: S. Kuehner :      kuehner-at-u.washington.edu
Date: Fri, 8 Mar 2002 08:10:02 -0800 (PST)
Subject: EDS software

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Good morning group- I am looking for an EDS program that can calculate a
spectrum from a chemical composition. I know that DTSA does that
calculation but it appears that the analyses must be entered one at a
time. I'd like to enter data in batch from a spreadsheet. Any ideas on
this?? thanks, scott

************************************************
....amphiboles do violence to history...
T. Feininger, 2001. (taken out of context)
****************************

Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************



From daemon Fri Mar 8 10:17:10 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 08 Mar 2002 11:05:32 -0500
Subject: Bio-SEM references

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Listers'
We are searching for recent papers which use FEG-SEM or cryo FEG-SEM to image mammalian tissues....preferably cytoskeleton and even more preferably drosophila...but any tissue will do.
I will be hitting the normal journals but do not expect to find a lot and, with the possibility that I might miss some, would appreciate your sending me any references you have readily at hand.
This is to help convince a group of researchers, who use TEM but have no experience with SEM, that this instrumentation may be useful to them for their research. Most of our users are plant or materials people.

Many thanks,
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Fri Mar 8 10:44:35 2002



From: Antonio Correia :      antonio-at-cmp-cientifica.com
Date: Fri, 8 Mar 2002 17:50:34 +0100
Subject: TNT2002 Nano Conference (Santiago de Compostela - Spain)

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Dear Colleague,

On behalf of the Organising Committee, I take great pleasure in announcing
you the third "Trends in NanoTechnology" International Conference (TNT2002)
which will be held in the City of Santiago de Compostela (Spain): September
09-13, 2002.The aim of TNT2002 is to focus on the applications of
Nanotechnology by bringing together various groups working in this field
from the USA, Japan and Europe. This year, a one day SRC/TNT Nanoelectronic
workshop will be organised and be an integrated part of TNT conference. The
workshop will be structured to encourage an active interchange of ideas
among participants. Six major nanoelectronics themes have been identified,
each of which will be addressed by a speaker from industry and a speaker
from a university. - at this stage speakers from IBM, HP, Samsung or Intel
already accepted to participate.

Full details available at:
http://www.cmp-cientifica.com/TNT2002.html

Regards

Antonio

KEYNOTE LECTURES (confirmed): 1. Masakasu Aono (Riken, Japan), 2. Phaedon
Avouris (IBM, USA), 3. Flemming Besenbacher (Aarhus University, Denmark), 4.
Guillermo Bozzolo (NASA Glenn Research Center, USA), 5. George Bourianoff
(Intel, USA), 6. Roberto Car (Princeton University, USA), 7. Ignacio Cirac
(University of Innsbruck, Austria), 8. Dongmin Chen (Rowland Institute for
Science, Cambridge, MA, USA), 9. Wonbong Choi (Samsung, Korea), 10. Harold
Craighead (Cornell University, USA), 11. Supriyo Datta (Purdue University,
USA), 12. Cees Dekker (Delft University, Netherlands), 13. Pedro Echenique
(DIPC, Spain), 14. Andreas Engel (Basel University, Switzerland), 15. Leo
Esaki (Shibaura Institute of Technology, Japan), 16. Fernando Flores
(Universidad Autonoma de Madrid, Spain), 17. Harald Fuchs (Munster
University, Germany), 18. Christoph Gerber (IBM, Switzerland), 19. James
Gimzewski (UCLA, USA), 20. James Heath (UCLA, USA), 21. Christian Joachim
(CEMES/CNRS, France), 22. Sajeev John (University of Toronto, Canada), 23.
Dieter Kern (Tuebingen University, Germany), 24. Uzi Landman (Georgia
Institute of Technology, USA), 25. Daniel Loss (Basel University,
Switzerland), 26. Ramesh G. Mani (Harvard University, USA), 27. Neil D.
Mathur (University of Cambridge, UK), 28. Meyya Meyyappan (NASA, USA), 29.
Seizo Morita (Osaka University, Japan), 30. Rodolfo Miranda (Universidad
Autónoma de Madrid, Spain), 31. Jan van Ruitenbeek (Leiden University,
Netherlands), 32. Lars Samuelson (Lund University, Sweden), 33. Christian
Schoenenberger (Basel University, Switzerland), 34. Ivan Schuller
(University of California, USA), 35. Clivia Sotomayor Torres (Wuppertal
University, Germany), 36. Christian Urbina (CEA-Saclay, France), 37. Luis
Vina (Universidad Autonoma de Madrid, Spain), 38. Mark Welland (University
of Cambridge, UK), 39. Stanley Williams (HP, USA)


Dr. Antonio CORREIA - Coordinator of the IST Nanoelectronics Network
(PHANTOMS)
CMP Cientifica S.L.
Phone: +34 91 6407187 Fax: +34 91 6407186
mailto:antonio-at-cmp-cientifica.com
WEB site: http://www.cmp-cientifica.com/
PHANTOMS WEB site: http://www.phantomsnet.com/




From daemon Fri Mar 8 10:57:25 2002



From: akc-at-umich.edu
Date: Fri, 08 Mar 2002 11:50:15 -0500
Subject: Re: Bio-SEM references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debbie,

On PubMed you might want to search for some of the work of Keiichi Tanaka,
who over the years has published some remarkable FEG-SEM of organelles
inside cells. A recent example is: Tanaka K, Fukudome H, 1991,
3-Dimensional organization of the Golgi complex observed by scanning
electron microscopy, J Electron Microsc Technique 17(1):15-23.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Friday, March 08, 2002 11:05 AM -0500 Debby Sherman
{dsherman-at-purdue.edu} wrote:

} Listers'
} We are searching for recent papers which use FEG-SEM or cryo FEG-SEM
} to image mammalian tissues....preferably cytoskeleton and even more
} preferably drosophila...but any tissue will do. I will be hitting the
} normal journals but do not expect to find a lot and, with the possibility
} that I might miss some, would appreciate your sending me any references
} you have readily at hand. This is to help convince a group of
} researchers, who use TEM but have no experience with SEM, that this
} instrumentation may be useful to them for their research. Most of our
} users are plant or materials people.
}
} Many thanks,
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907
}





From daemon Fri Mar 8 13:59:00 2002



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Fri, 08 Mar 2002 14:28:52 -0500
Subject: Parts for an old coating unit

Contents Retrieved from Microscopy Listserver Archives
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Hi, all,

We inherited an Edwards E306A vacuum evaporator awhile ago. Over the years, we have tried to get it going using suggestions from the list and particularly from Chris Smith (thanks, Chris!). Our in-house engineers and local electricians finally figured out that the rheostat module was not working. Of course that's the most expensive bit. We cannot afford to replace the whole coating unit but would like to try to repair the old one if possible.

My question: does anyone have a rheostat module for the E306A that they are willing to donate/sell? If so, please contact me offline with a price and other details.

Thanks in advance.

Paula.



Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Fri Mar 8 15:06:38 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Fri, 8 Mar 2002 14:56:27 -0600
Subject: RE: EDS software

Contents Retrieved from Microscopy Listserver Archives
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Hello Scott,

Don Chernoff at "Small World" sells Electron Flight Simulator (windows pgm)
which can construct a spectrum from a hypothetical matrix. The www link is
not handy, but if you can't find it let me know. If memory serves, it is
about $800.

Regards, Woody
Woody White
SEM-EDS-WDS
McDermott Technology, Inc
-------------------------
McDermott site: http://www.mtiresearch.com/
Personal site: http://woody.white.home.att.net


} -----Original Message-----
} From: S. Kuehner [mailto:kuehner-at-u.washington.edu]
} Sent: Friday, March 08, 2002 11:10 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: EDS software
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
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} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} Good morning group- I am looking for an EDS program that can
} calculate a
} spectrum from a chemical composition. I know that DTSA does that
} calculation but it appears that the analyses must be entered one at a
} time. I'd like to enter data in batch from a spreadsheet.
} Any ideas on
} this?? thanks, scott
}
} ************************************************
} ....amphiboles do violence to history...
} T. Feininger, 2001. (taken out of context)
} ****************************
}
} Dr. Scott Kuehner kuehner-at-u.washington.edu
} Dept. of Geological Sciences ph.206-543-8393
} Box 351310 Fax 206-616-6873
} The University of Washington
} Seattle, Washington 98195-1310
} ************************************************
}
}


From daemon Fri Mar 8 18:44:02 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 8 Mar 2002 16:28:19 -0800
Subject: Recirculating units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all:

So, the compressor on the cooling unit for our SEM conked out. Now I need
to look into a replacement. Here are some details:

We were using a Neslab CFT-75 until it flaked out. The SEM is an ISI WB-6,
a medium sized SEM. ISI called for 2 l/min, 20 C. The tech who told me the
Neslab was toast said it was a 9000BTU/hr unit and was hardly working to
keep up with the load. It was actually working constantly, these units run
the compressor all the time and control the temp by using a hot gas bypass
system to keep the temp = +/- 0.1 C.

I can repair the Neslab with a new compressor for about $1K. Still will
have all the other old parts, pump, electronics etc., it is 20 years old,
and a way over capacity unit for the job.

I can buy a new refrigerated unit, but not sure how low I can go on the BTU
capacity. I am a cheapskate and would like to keep the cost as low as
possible while still getting adequate cooling. I have looked around for
different units and have found several sources and brands. Prices are from
$2+K to $4K. Let me know if you have a preferred source I may have
overlooked.

A real cheap way to go is a self-contained liquid to air cooling unit from
McMaster-Carr. It is less than half the cost of the other recirculators,
but it does not use a compressor for cooling. Kind of like an independent
radiator and fan as found in your automobile.

I have another CFT-75 I have put on the ISI for now. It used to be used for
a Denton VE. One thought I had was to keep the second CFT-75 on the SEM,
better cooling, more controlled temp., etc. and get a cheapo cooling unit
for the VE. Maybe the McMaster unit would handle the VE, after all, some
DP's are simply air cooled. The heat load and temp. requirements for a
simple VE aren't to strict, at least I don't think so. Denton only
specified 200 cc/min. I cna't find any info. calling out the heat loads for
the SEM or VE in BTU/hr terms.

Any brainiac heating/cooling experts want to put your 2 cents in?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Mar 8 18:57:02 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 8 Mar 2002 17:55:00 -0700
Subject: 419 scam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

I am getting a number of emails where I am promised millions of Dollars.
These emails seem to be connected with the list server as I have seen email
addresses from other listers also in the header. Perhaps somebody is
collecting email addresses from the postings. If you receive the same type
of emails (its' usually someone in Nigeria who wants to transfer illicit
money into the US and requires your help for a good chunk of the money),
there is a web site that explains the whole scam.

http://home.rica.net/alphae/419coal/

Have a nice weekend, everybody.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================




From daemon Fri Mar 8 21:50:49 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Fri, 8 Mar 2002 21:40:48 -0600
Subject: Manual or Automatic Critical Point Dryer?

Contents Retrieved from Microscopy Listserver Archives
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I am setting up an SEM lab for a Biology Dept. I have been advised
that a Manual Critical
Point Dryer is easier to maintain than the Automatic kind, and allows
one to control the drying
process more readily. But I have also heard that some specimens
(insect?!?) can take hours
rather than minutes to process, making the automatic CPD worth having
for the Dept. Is this
true?


From daemon Fri Mar 8 21:50:49 2002



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu
Date: Fri, 8 Mar 2002 21:40:24 -0600
Subject: High Pressure Freezer input sought

Contents Retrieved from Microscopy Listserver Archives
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Hello All,
We are hoping to add a High Pressure Freezer instrument to our stable of
techniques. We are looking for any and all feedback people might have in
regards to the systems currently available on the market.
Thanks in advance for your time,

Randy Nessler
CMRF Associate Director
University of Iowa
Iowa City, IA 52242
Phone 319-335-8142
http://www.uiowa.edu/~cemrf


From daemon Sat Mar 9 09:41:22 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 09 Mar 2002 10:33:26 -0500
Subject: Manual vs. automatic CPD units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Greg Barclay wrote:
=========================================================
I am setting up an SEM lab for a Biology Dept. I have been advised
that a Manual Critical
Point Dryer is easier to maintain than the Automatic kind, and allows
one to control the drying
process more readily. But I have also heard that some specimens
(insect?!?) can take hours
rather than minutes to process, making the automatic CPD worth having
for the Dept. Is this
true?
========================================================
I have never heard of anyone finding that an "automatic" was faster in that
respect than a "manual" unit. After all, the same "physics" in terms of
the exchange of the liquids is going to apply in either case.

Some models of "automatic" units come with "agitation" (which could lead to
faster exchange times). I came out of the "old school" believing that this
should all be done under conditions of laminar flow, and the desirability of
having a large sight glass was in part to be able to confirm that indeed
there was no turbulence and only laminar flow. Now am I wrong about this
and that all of a sudden agitation (sort of the antithesis of laminar flow)
is desirable? I would have thought that for fragile biological samples, for
example, this would be potentially detrimental and at the least, would cause
potential uncertainty in one's final results.

Disclaimer: Although our firm now offers both, I am myself interested in
knowing the actual experience of others when comparing units with agitation
vs. no agitation.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================


From daemon Sat Mar 9 10:53:01 2002



From: josvarelas-at-netscape.net ()
Date: Sat, 9 Mar 2002 10:42:42 -0600
Subject: Ask-A-Microscopist: incorporating dimethyl sulfoxide into

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (josvarelas-at-netscape.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
March 7, 2002 at 15:38:57
---------------------------------------------------------------------------

Email: josvarelas-at-netscape.net
Name: Joseph Varelas

Organization: Lockheed-Martin

Education: Undergraduate College

Location: Mountain View, CA

Question: How would I go about incorporating dimethyl sulfoxide
(DMSO) into a fixation protocol for TEM?
Do I use a percentage of the solvent in the fixative and subsequent
buffer washes?

---------------------------------------------------------------------------


From daemon Sun Mar 10 19:18:44 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 11 Mar 2002 13:55:06 GMT+1200
Subject: Re: Parts for an old coating unit

Contents Retrieved from Microscopy Listserver Archives
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Paula

I have a 306, in regular use.

No spares, sorry, but mine, at least, has no rheostat, and I doubt
that yours has, unless they changed it markedly for the US market..

Mine has a variable transformer to control the evaporation current,
which is a much more practicable way to do it than a rheostat.

You should be able to buy a new one that will work OK from a local
electrical supply house, it may not fit into where the original does
but you could wire it external to the coater. I would guess that it
would cost around $100, not that expensive, certainly cheaper than a
new rotary pump or a new bell jar! I know because I broke my bell jar
a little while back.

Have you priced a replacement one from Edwards?

It may be your best option.

cheers

rtch





} Date: Fri, 08 Mar 2002 14:28:52 -0500
} From: "Paula Allan-Wojtas" {AllanWojtasP-at-EM.AGR.CA}
} To: {microscopy-at-sparc5.microscopy.com}
} Subject: Parts for an old coating unit

} --------------------------------------------------------------------
} ---- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
} ---.
}
}
} Hi, all,
}
} We inherited an Edwards E306A vacuum evaporator awhile ago. Over the
} years, we have tried to get it going using suggestions from the list
} and particularly from Chris Smith (thanks, Chris!). Our in-house
} engineers and local electricians finally figured out that the
} rheostat module was not working. Of course that's the most expensive
} bit. We cannot afford to replace the whole coating unit but would
} like to try to repair the old one if possible.
}
} My question: does anyone have a rheostat module for the E306A that
} they are willing to donate/sell? If so, please contact me offline
} with a price and other details.
}
} Thanks in advance.
}
} Paula.
}
}
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca
}
}
Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Sun Mar 10 21:33:15 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Sun, 10 Mar 2002 19:23:43 -0500
Subject: Re: Recirculating units

Contents Retrieved from Microscopy Listserver Archives
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on 3/8/02 7:28 PM, Jon Krupp at jmkrupp-at-cats.ucsc.edu wrote:
}
} So, the compressor on the cooling unit for our SEM conked out. Now I need
} to look into a replacement. Here are some details:
}
} We were using a Neslab CFT-75 until it flaked out. The SEM is an ISI WB-6,
} a medium sized SEM. ISI called for 2 l/min, 20 C. The tech who told me the
} Neslab was toast said it was a 9000BTU/hr unit and was hardly working to
} keep up with the load. It was actually working constantly, these units run
} the compressor all the time and control the temp by using a hot gas bypass
} system to keep the temp = +/- 0.1 C.
}
} I can repair the Neslab with a new compressor for about $1K. Still will
} have all the other old parts, pump, electronics etc., it is 20 years old,
} and a way over capacity unit for the job.
}
Just as the old mechanical vacuum pumps on the HVEM have been working
steadily for over 20 years, and the newer, direct-drive ones do not last
nearly that long, I suspect the same will be true of your old cooling unit.
My inclination would be to replace the compressor, and, of course, keep up
preventive maintenance on the system. The hot-gas bypass is an excellent
thermostabilizer, and, like many other pieces of equipment, running the unit
continually under light load conditions is vastly easier on the components
than any other regime.

} I can buy a new refrigerated unit, but not sure how low I can go on the BTU
} capacity.

This can, of course, be calculated from the heat output of your SEM and
the flow rate. Don't run a (say) 1 kBTU/hr unit at anywhere that heat flow,
or it will wear out faster, fail to give good thermal stability, and be
unable to cope with changes in the heat generated by the SEM, should
anything go wrong. Replacing a smaller cooling unit every few years is a
false economy.
}
} A real cheap way to go is a self-contained liquid to air cooling unit from
} McMaster-Carr. It is less than half the cost of the other recirculators,
} but it does not use a compressor for cooling. Kind of like an independent
} radiator and fan as found in your automobile.
}
Does it give +/- 0.1 C? The more stable the temp, the better the
images.

} I have another CFT-75 I have put on the ISI for now. It used to be used for
} a Denton VE. One thought I had was to keep the second CFT-75 on the SEM,
} better cooling, more controlled temp., etc. and get a cheapo cooling unit
} for the VE. Maybe the McMaster unit would handle the VE, after all, some
} DP's are simply air cooled. The heat load and temp. requirements for a
} simple VE aren't to strict, at least I don't think so. Denton only
} specified 200 cc/min. I cna't find any info. calling out the heat loads for
} the SEM or VE in BTU/hr terms.

Perhaps the manufacturer or a service person would know the appropriate
numbers.
}
} Any brainiac heating/cooling experts want to put your 2 cents in?
}
Done.
Yours,
Bill Tivol



From daemon Mon Mar 11 07:43:51 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Mon, 11 Mar 2002 07:35:20 -0600
Subject: RE: Parts for an old coating unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Paula,

Rheostat, potentiometer, and variable autotransformer are three different
devices whose names are commonly (and incorrectly) interchanged.

As the other poster said, it is most likely a "variable autotransformer".
You will need to confirm what is actually being used.

The most common failure mode for this device is a worn brush (contact).

Woody

--------------

} -----Original Message-----
} From: Paula Allan-Wojtas [mailto:AllanWojtasP-at-EM.AGR.CA]
} Sent: Friday, March 08, 2002 2:29 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Parts for an old coating unit
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi, all,
}
} We inherited an Edwards E306A vacuum evaporator awhile ago.
} Over the years, we have tried to get it going using
} suggestions from the list and particularly from Chris Smith
} (thanks, Chris!). Our in-house engineers and local
} electricians finally figured out that the rheostat module was
} not working. Of course that's the most expensive bit. We
} cannot afford to replace the whole coating unit but would
} like to try to repair the old one if possible.
}
} My question: does anyone have a rheostat module for the E306A
} that they are willing to donate/sell? If so, please contact
} me offline with a price and other details.
}
} Thanks in advance.
}
} Paula.
}
}
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca
}
}


From daemon Mon Mar 11 08:38:54 2002



From: Teresa Flores :      tflore-at-lsuhsc.edu
Date: Mon, 11 Mar 2002 08:37:10 -0500
Subject: Re-embedding thick section

Contents Retrieved from Microscopy Listserver Archives
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Rick asked the following:
} What is your favorite method for treating glass slides for thick sections
} such that they can then be re-embedded? We re-embed by standing a
} polymerized BEEM block on top of the section with a drop of epoxy between
} the two. Without treating the slide first, it is often difficult to remove
} the newly joined section and block.
Rick, whenever our EM Lab has to re-embedding the "thick" section we use a
BOJAK MOLD. This BOJAK MOLD was also used in our STAT Microwave technique.
REF:The Journal of Histotechnology /Vol 21, No. 3 September 1998.
The coverslip of the "thick" section must be removed first then the section
is aligned with the area needed in thick section and epoxy is placed in
well needed, slide is slid into place, where the section is, right over the
epoxy well and then bojak mold with slide on top is placed in a 70oC oven
overnight and in the AM is just popped off and area needed on thick section
remains on the hardened epoxy block ready for thin sections.
We also use this technique when TEM is needed and only an H&E slide is
available and no tissue for EM.





From daemon Mon Mar 11 09:48:55 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Mon, 11 Mar 2002 10:41:22 -0500
Subject: Bio-SEM references

Contents Retrieved from Microscopy Listserver Archives
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you might try pubmed....thats where i always look. and it's free.


From daemon Mon Mar 11 09:53:33 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Mon, 11 Mar 2002 10:47:33 -0500
Subject: resharpening

Contents Retrieved from Microscopy Listserver Archives
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wetting of a diamond knife is a common problem and not really related to the
sharping process. it's a feature of diamonds tover glass they are hydrophobic
by nature. it's one way of telling diamonds from glass. a trick i learned manu
moons ago, i am sure will be lookded down on, is to wet your eyelash with a
little salivia and run it along the edge of the knofe before filling the boat
with water. if you just havn't finished eating lunch the boat water will
remain free of contamination and the edge will wet nicely.
john


From daemon Mon Mar 11 09:55:23 2002



From: Robert Wieland :      wieland-at-me.udel.edu
Date: Mon, 11 Mar 2002 10:50:16 -0500 (EST)
Subject: RE: Parts for an old coating unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"White, Woody N." {nwwhite-at-mcdermott.com} wrote:

} Rheostat, potentiometer, and variable autotransformer are three
} different devices whose names are commonly (and incorrectly)
} interchanged.
}
} As the other poster said, it is most likely a "variable
} autotransformer". You will need to confirm what is actually being used.
}
} The most common failure mode for this device is a worn brush (contact).
}
} Woody

If it is one of the larger autotransformers (diameter greater than
about six inches), it has an internal fuse underneath the black plastic
plate on which the electrical connections are mounted. Burnout of this
fuse is, in my experience, the usual cause of abrupt & 'quiet' failure.
The wearout of the sliding contact will result in a long period of
increasingly-erratic operation before failure; burnout of the winding will
fill the lab with the odor of scorched insulation.
If your fuse has blown, and you cannot find a replacement (I couldn't),
just wire in an external fuse & holder, and jumper over the blown internal
fuse. But its 'bad luck' to operate without any fuse.

These autotransformers are pretty much alike in their manufacture; any
two units of the same physical size will have nearly the same power &
current ratings. If you need a new unit, just match the size & the
operating voltage (both things you can measure), and you needn't worry
about discovering the other specs. The only 'strangeness' that you might
run into is that a very few military surplus autotransformers were made
for 400-Hz power; they won't do on 50/60-Hz power (without
'derating').
You can save big money buying these on the surplus market, but stay
away from custom & military production items; the touchstone is that the
unit should state on the connection plate its voltage, current, &
frequency ratings. You're gambling on anything that is labelled with only
a part number.





From daemon Mon Mar 11 11:53:55 2002



From: LEVIN,MARTIN A. (Biology) :      LEVIN-at-easternct.edu
Date: Mon, 11 Mar 2002 12:45:23 -0500
Subject: LKB Ultrotome Nova Repair

Contents Retrieved from Microscopy Listserver Archives
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I have an LKB Ultrotome Nova. It needs a couple of minor repairs and is
overdue for a general cleaning. Can anyone tell me who is still servicing LKB microtomes? Thanks

Martin A. Levin
Director of the Center for Educational Excellence (CEE)
and Professor of Biology
Eastern Connecticut State University
J. Eugene Smith Library, Room 431
Willimantic, Connecticut 06226
(860)465-5589/4324
FAX (860)465-5522/5213
Email: levin-at-easternct.edu




From daemon Mon Mar 11 15:12:04 2002



From: Bob Harris :      bharris-at-micro.uoguelph.ca
Date: Mon, 11 Mar 2002 16:03:24 -0500
Subject: Help for Philips EM 300

Contents Retrieved from Microscopy Listserver Archives
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Hello all:

WANTED: We are hoping to keep our old Philips 300
operational. To do so we need a conversion kit to remove the
Mercury Pump. If anyone knows of a kit that's available or possibly
an entire Hg-less EM300 please let me know. Thank you, bob
Bob Harris
NSERC Regional STEM Facility
Dep't of Microbiology
University of Guelph
Guelph Ontario
Canada N1G 2W1
Phone: 519-824-4120 ext 6409
Fax: 519-837-1802


From daemon Mon Mar 11 15:14:52 2002



From: Earl Weltmer :      earlw-at-sbcglobal.net
Date: Mon, 11 Mar 2002 15:07:05 -0600
Subject: ADEM Manuals

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

I have some manuals etc from Tracor's ADEM microscope, free to anyone who
will pay shipping.

Please contact me offline if interested.

Thank You,

Earl Weltmer


From daemon Mon Mar 11 16:59:25 2002



From: Christina Chan :      snowchan-at-leland.stanford.edu
Date: Mon, 11 Mar 2002 14:51:23 -0800
Subject: EM pics of Poliovirus

Contents Retrieved from Microscopy Listserver Archives
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I was given this address as a source for EM information, but I am not
trained in EM _at all_...so please forgive what may be basic
questions:

1. What is the procedure used for taking EM pictures of poliovirus
(RNA virus)?
2. Is it possible to calibrate the number of genomes (capsulated RNA
viruses as well as free floating RNA) in a known aliquot of virus
dilution?
3. Is it difficult to recognize poliovirus RNA in its capsulated and
free floating forms in an EM picture?

Our lab is trying to find the number of genomes of poliovirus in a
stock solution of 10^8 TCID50/1ml. Any help or advice I could get
would be greatly appreciated!!

Thanks,
Christina Chan
--
LSRA - Lab Manager
Maldonado Lab
Pediatrics, Infectious Disease
Stanford University Medical Center
lab: (650) 736-1310
cell: (650) 996-0098


From daemon Mon Mar 11 16:59:26 2002



From: hlwil-at-webtv.net (Harold & Arvella)
Date: Mon, 11 Mar 2002 16:50:49 -0600
Subject: SWIFT 960 Ser. straight microscope

Contents Retrieved from Microscopy Listserver Archives
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I have an older SWIFT 960 Ser. straight
microscope, that i do not know anything
about, it has a #822941 on scope, the
eyepiece has 10x on it but the objectives
that screw into the turret have no numbers
on them, their is only 2 objectives, the
third is open, i am going to sell the scope
but i do not know how to describe the
magnifications.
can anyone help me with this problem?
TIA Harold


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Content-Type: Text/HTML; Charset=US-ASCII
Content-Transfer-Encoding: 7Bit

{http://www.wunderground.com/US/FL/Holiday.html}


From daemon Mon Mar 11 19:53:55 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 11 Mar 2002 17:42:25 -0800
Subject: Re: Parts for an old coating unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When I built my own vacuum evaporator system, I was using standard
laboratory 'variable autotransformer' and another standard two-coils power
transformer. Autotransformer powered the primary 'coil' of the
transformer. Secondary coil (100A max) is connected to the evaporation
device. I got autotransformer from the our university 'junk-yard' and got
power transformer for $20 from some electrical supplier. Autotransformer
is 4.5" dia and fuse is 12A. Perhaps, your system utilized the same
two-transformer design (very popular in the past). If so, it's very
unlikely that power transformer is fail and as mentioned other colleagues,
you may replace 'variable autotransformer' using any kind with suitable
characteristics... If you need details, you could contact me off-line. Sergey

At 05:55 AM 3/11/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Mar 11 20:26:34 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Mon, 11 Mar 2002 21:24:15 -0800
Subject: Re: Sectioning buds in agarose (fwd)

Contents Retrieved from Microscopy Listserver Archives
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} Dear Listers,

Thanks to those who responded. Included in this email are helpful
comments from
Mark P. Running, Fred Monson and Tamara Howard. We are using a bioslicer
and have had some success with inflorescences embedded in 7% agarose.
Rosemary

} I am familiar with the technique and met the woman who invented it at a
} Keystone meeting last year. I'm quite certain she'd be happy to answer
} any questions directly; her name is Hinanit Koltai, and her email is
} hinanit_koltai-at-ncsu.edu. If I recall she was aiming for sections of
} 40-100 um thickness, but used a standard paraffin microtome. I'm not sure
} if you have the full reference for the protocol, but it is: Hinanit Koltai
} and David McKenzie Bird (2000). High Throughput Cellular Localization of
} Specific Plant mRNAs by Liquid-Phase in Situ Reverse
} Transcription-Polymerase Chain Reaction of Tissue Sections. Plant
} Physiol. 123: 1203-1212.
}
} While it is rapid, there is some concern that this technique shows overly
} broad expression patterns when compared to traditional in situs on fine
} sectioned tissue.
}
} Hope this helps.
}
} Mark P. Running, Ph.D.
} Assistant Member, Principal Investigator
} Donald Danforth Plant Sciences Center
} 975 N Warson Rd
} Saint Louis MO 63132
} Phone(314) 587-1641
} Cell(314)359-9344
} Fax(314) 587-1741
} mrunning-at-danforthcenter.org
Hi Rosemary,
As many times as I have been called to a cryostat to help with a
problem with variable section thicknesses forces me to respond to your
query.
In almost every case, the problem was relieved by increasing the
stability of the specimen-block axis. That is, tightening up the vices and
holders. In one case, I removed the microtome, which had not been cleaned
for years and cleaned and oiled it with low-Temp oil. On that microtome,
much was loose, including the bearings on the vertical support of the
specimen arm. I even tightened the specimen arm a little after trying the
sectioning. Most of the time, if the microtome has been used regularly, and
the operator is practiced, there is something about the mount.
Now about the agarose. It's an interesting choice, and I understand
it for the purpose, but it creates a very fragile gel. I am led to ask a
couple questions.
1. Any chance to query the publisher of the method for the
specifics of his/her system. Knife? Cryostat?
2. What kind of knife are you using? If you are using a
disposable, you might find a hollow-ground non-disposable knife better. I
have used these in the past to section specimens embedded in 1-4% acrylamide
gels and have been pleased with the results. The downside, of course, is
that the knife dulls more quickly that non-hollow ground edges.
3. Another thing is that users tend to take a chunk of embedded
(tissue and just 'glue' it to the specimen mount on the cryotome holder.
Sometimes, the sequence leaves the specimen unstable, and there is a
requirement to change the method by which the specimen is attached. I don't
know what prohibitions there are at this point, but a rectangular block of
agarose frozen to a stub with water will NOT provide the kind of stability
for the block that is normally recommended.
4. Finally, the 40um section suggests that for each sectioning
stroke there is a collision of the knife edge with the block. Frozen will
be OK, but on the edge of the thaw would be better. For sections that
thick, I would be running the cryostat at -10 or -8. Also, I would permit
the block lots of time - 2hr - to come to equilibrium with the chamber
temperature. You might also want to consider using a sledge microtome,
because there are two knife approach angles to set. The sectioning stroke
brings the knife in a slicing motion through the specimen, and both angles
are variable.
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


Is is probably a protocol for a vibratome...if you don't have access to
one, maybe make sections by hand? You can get those rodent brain slicing
molds (they have little grooves for the blade to go in at set distances
apart) from the EM supply companies, or do the homemade version several
ways -
quick and dirty would be to attach several sharp blades at set distances
on a handle of some kind. I've seen homemade choppers of several
double-edge blades broken in 2, taped to a heavy stick with spacers in
between each blade. Then just cut as usual.
Or go fancier - someone showed me a homemade chopper that was pretty much
a mini-hand-vibratome....they had a micrometer advance thingy (there
is a word for this but my memory is toast) hooked up on a sort of a seesaw
over a pivot point, with the blade on the other end of the seesaw, such
that you could put this gizmo down on a surface (wax) with your piece of
tissue under the blade, rock the blade down and make a cut, then advance
the blade using the micrometer thingy a defined distance, cut again, etc.
Good luck!
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu



From daemon Tue Mar 12 08:33:36 2002



From: zaluzec-at-microscopy.com
Date: Tue, 12 Mar 2002 08:20:47 -0600
Subject: Fwd: Ask-A-Microscopist: How to make Microscope "slides" for High

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Date: Mon, 11 Mar 2002 16:47:49 -0600 (CST)
} To: Zaluzec-at-sparc5.microscopy.com
} From: michelbruneau-at-hotmail.com ()
} Subject: Ask-A-Microscopist
} Status:
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (michelbruneau-at-hotmail.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} March 11, 2002 at 16:47:48
} ---------------------------------------------------------------------------
}
} Email: michelbruneau-at-hotmail.com
} Name: michel bruneau
}
} Organization: high school
}
} Education: 9-12th Grade High School
}
} Location: Canada
}
} Question: I am a high school science teacher. I require either a
} website or a protocol to have high school kids be able to make
} permanent mount glass slides for the old fashion microscope in orcer
} to create a permanent collection for the school. All I have found
} so far is wet mount slides which is not the type I require. Any
} suggestion would be helpful. Thanks.
} Mike
} michelbruneau-at-hotmail.com
}
} ---------------------------------------------------------------------------
}


From daemon Tue Mar 12 09:39:01 2002



From: Ed Calomeni :      ecalomeni-at-mco.edu
Date: Tue, 12 Mar 2002 10:31:13 -0500
Subject: LM: USED INVERTED MICROSCOPE

Contents Retrieved from Microscopy Listserver Archives
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Hi Folks,

Have a colleague that is in the process of setting up a lab and is in
the need of an used inverted microscope. Any person or company that
may have one please respond to this list or to me and I will forward
all.

thanks,

Ed Calomeni


From daemon Tue Mar 12 10:41:55 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 11 Mar 2002 15:37:08 -0800
Subject: Gloves

Contents Retrieved from Microscopy Listserver Archives
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I asked the list for information on the resistance of nitrile gloves to
unpolymerized embedding media; I got no response. Anyone with an interest
in their resistance to other chemicals will find a useful table at
http://www.cdc.gov/od/ohs/manual/pprotect.htm

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Mar 12 12:42:53 2002



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Tue, 12 Mar 2002 13:32:06 -0500
Subject: Parts for an old coating unit - thanks

Contents Retrieved from Microscopy Listserver Archives
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Hi, all,

I am overwhelmed by all the responses and helpful information which you sent - thanks! I am presently trying to arrange some time with the person who spent the most time trying to repair it to go through your replies. The first contact we had when we tried to enquire about buying the variable transformer (yes, that was what I meant) was that the part was way out of our price range, and that it might not be available any longer because the unit was so old. It was nice to hear that we can use "generic" parts which are relatively inexpensive.

Yes I have schematics for the unit. I will see what we can do with the resources you have provided, and if we have additional problems or questions, we will contact those of you who have offered.

Wish us luck!

Thanks again.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Wed Mar 13 08:48:02 2002



From: Bob Harris :      bharris-at-micro.uoguelph.ca
Date: Wed, 13 Mar 2002 09:27:13 -0500
Subject: Two used JEOL EMs available

Contents Retrieved from Microscopy Listserver Archives
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Hello :

We have an operational, Series 1, JEOL 100CX, with STEM
attachments and an operating,but barely, JEOL 35C that will be
decommissioned in the near future and are available. If you are
interested please contact me directly. bob
Bob Harris
NSERC Regional STEM Facility
Dep't of Microbiology
University of Guelph
Guelph Ontario
Canada N1G 2W1
Phone: 519-824-4120 ext 6409
Fax: 519-837-1802


From daemon Wed Mar 13 09:17:18 2002



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Wed, 13 Mar 2002 09:11:28 -0600
Subject: Zeiss Axiovert 100 parts

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
I am interested in refurbishing some of the mechanical components in one of
our Zeiss Axiovert 100 microscopes. The focusing mechanism has taken some
abuse through the years and it appears that a savage managed to strip some
teeth on the focusing rack. I would like to replace the brass rack, and
the coarse/fine focus spindle. Our fine focus is manipulated using a
motorized z-control-it appears that the fine focus is coupled to the course
focus gearing through a ball bearing friction mechanism. This mechanism
slips-I would be interested in replacing it with a planetary gear assembly
for better z-control precision/repeatability if I could find the parts. If
this proves to be unpractical I will be interested in simply
replacing/tuning the assembly.
The Zeiss Axiovert 100 is a discontinued microscope base, and I
have been reassured by our sales rep that parts are available, however I
have had little success getting any quotes or getting a call-back from a
service rep over the past couple of months. I would like to find out if
any resources for obtaining remanufactured (cannibalized), new but
discontinued, or third party engineered (or re engineered) parts for Zeiss
microscopes. I would be interested in hearing from independent microscope
service engineers who work with Zeiss products as well. Thanks in advance
for your kind assistance.
Sincerely,
Karl G.

_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Wed Mar 13 10:05:10 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 13 Mar 2002 10:05:29 -0600
Subject: old Edwards freeze-dryer

Contents Retrieved from Microscopy Listserver Archives
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Does anyone still have manuals for the old Edwards Model EPTD 3
"tissue dryer" freeze-dryer? We've just scavenge one, but need to
find a copy of the manual(s).

Thanks!

Phil
--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Wed Mar 13 10:06:42 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 13 Mar 2002 10:08:44 -0600
Subject: H-Nu EDX system

Contents Retrieved from Microscopy Listserver Archives
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Another request: does anyone have service manuals (not operator
-we've got that) for the old H-Nu EDX system? And -- I hope I hope --
copies of the System 5000, version 3.7 software on 3.5" floppies?

Thanks again!

Phil
--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Wed Mar 13 11:09:14 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 13 Mar 2002 08:59:20 -0800
Subject: Re: Fwd: Ask-A-Microscopist: How to make Microscope "slides" for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } Email: michelbruneau-at-hotmail.com
} } Name: michel bruneau
} }
} } Organization: high school
} }
} } Education: 9-12th Grade High School
} }
} } Location: Canada
} }
} } Question: I am a high school science teacher. I require either a
} } website or a protocol to have high school kids be able to make
} } permanent mount glass slides for the old fashion microscope in orcer
} } to create a permanent collection for the school. All I have found
} } so far is wet mount slides which is not the type I require. Any
} } suggestion would be helpful. Thanks.
} } Mike
} } michelbruneau-at-hotmail.com
} }
} } ---------------------------------------------------------------------------
Mike - -

If you're hoping for fixed, sectioned, stained mammalian tissues, that will
probably be beyond your students' capabilities. If you want whole mounts
of things like insect wings, diatoms, etc., that's possible. I suggest
that you get a copy of:
-----------------------
Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy; it's unequalled as a
basic reference for beginners. Almost half of the book is devoted to
simple preparation methods for biological specimens and descriptions (with
good illustrations) of commonly encountered organisms. Adult. RECOMMENDED
-----------------------
It will also tell you about your "old fashion microscope". The book
description is taken from the Project MICRO bibliography (URL below);
you'll find a lot of useful websites there too.

Where are you located in Canada? Perhaps we can find a member of the
Microscopy Society of Canada who can advise you.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Mar 13 14:14:01 2002



From: S Keller :      swtkeller-at-yahoo.com
Date: Wed, 13 Mar 2002 12:05:12 -0800 (PST)
Subject: Looking for used 200 CX TEM w/ STEM or similar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:
I am looking to acquire a 200 CX TEM w/ STEM or
similar in good working condition. The less expensive
the better.
Thanks in advance,
Sandra


__________________________________________________
Do You Yahoo!?
Try FREE Yahoo! Mail - the world's greatest free email!
http://mail.yahoo.com/


From daemon Wed Mar 13 14:44:09 2002



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Wed, 13 Mar 2002 14:33:04 -0600
Subject: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
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Hello, listers,

I am using an FESEM (Hitachi S-4000). I've noticed a disparity in
magnification calibration between the X and Y directions. I get about 1.5
percent error in the Y direction, and almost 5 percent in the X direction.
Because the percentages still fall within 10 percent, the field service
engineer believes that this is not a problem.

What do you think? Thanks in advance for your input.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Wed Mar 13 15:21:36 2002



From: Ken Bart :      kbart-at-hamilton.edu
Date: Wed, 13 Mar 2002 16:16:26 -0500
Subject: Stereology Software

Contents Retrieved from Microscopy Listserver Archives
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Hi Folks:
Can anyone make a recommend software to make 3D
reconstructions from serial TEM and LM images? Thanks for all
suggestions!

Ken
--


Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323

Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu
http://academics.hamilton.edu/biology/kbart/


From daemon Wed Mar 13 15:22:42 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 13 Mar 2002 16:17:13 -0500
Subject: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It's not going to get fixed unless you bitch about it. But do it in a nice way so that they you know that it is an issue with you and be insistent. If they can adjust it, they should do it during the next routine.

In the interim, you can make the calibration perfect with digital processing. Just adjust the size in pixels appropriately in any of a number of software programs, e.g. Photoshop, ThumbsPlus, etc. If you paste the image into a word-processing or presentation program, you can also change the aspect ratios and sizes to make them perfect.

BTW, you do have the tilt correction off, right? I don't know which direction the Hitachi stage tilts, but if you have the correction on and no tilt, you can get an error.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Nancy Zjaba [mailto:nzjaba-at-alfalight.com]
Sent: Wednesday, March 13, 2002 3:33 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Hello, listers,

I am using an FESEM (Hitachi S-4000). I've noticed a disparity in
magnification calibration between the X and Y directions. I get about 1.5
percent error in the Y direction, and almost 5 percent in the X direction.
Because the percentages still fall within 10 percent, the field service
engineer believes that this is not a problem.

What do you think? Thanks in advance for your input.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Wed Mar 13 17:08:03 2002



From: Robin Scribailo :      RScrib-at-purduenc.edu
Date: Wed, 13 Mar 2002 16:39:07 -0600
Subject: analog to digital conversion

Contents Retrieved from Microscopy Listserver Archives
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Dear Listserver Members,


We have an old ETEC Autoscan SEM (1974) which we would like to covert from analog to digital. There are a number of companies that will do this conversion for around $10,000. I have heard that the hardware needed is actually minimal and can be accomplished for far less. I would appreciate any advice on this matter especially since money is an issue.

Robin

Robin W. Scribailo Ph.D.
Associate Professor of Biological Sciences
Director of the Aquatic Plant Herbarium
Biological Sciences
Purdue University North Central
1401 S. U.S. 421
Westville, IN 46391-9528
(219) 785-5255
Fax (219) 785-5483
rscrib-at-purduenc.edu



From daemon Wed Mar 13 17:54:04 2002



From: DULATT, KAREN R [AG/1000] :      karen.r.dulatt-at-Monsanto.com
Date: Wed, 13 Mar 2002 17:44:55 -0600
Subject: Postdoctoral Research Associate - Monsanto

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Monsanto values diversity and is an equal opportunity affirmative action
employer.

Department: Research & Development
Title: Postdoctoral Research Associate
Req Number: mons-00000241
Location(s): St. Louis MO
Responsibilities:

A two-year postdoctoral fellow position is available immediately for
developing, implementing, and applying advanced microscopy techniques to
elucidating the ultrastructure of plants, seeds, weeds and other biological
systems. Additionally, the selected individual is responsible for developing
new methods to improve the sample preparation protocols of biological
systems. The selected candidate will interact with multifunctional groups of
scientists working on biotechnology projects.
Required Skills:

The position requires a Ph.D. in plant biology or a related field with
experience in advanced electron and light microscopy techniques. A strong
background and extensive experience in TEM, high-resolution cryo-SEM, and
confocal laser scanning microscopy techniques are essential. Experience with
gene transformation in plants is a strong plus. The following key
competencies are desired: highly motivated and interested in developing new
imaging technologies; good interpersonal, verbal and written communication
skills; innovative and seeking opportunity to improve existing techniques
and processes.


From daemon Wed Mar 13 18:39:53 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 13 Mar 2002 16:35:56 -0800
Subject: Re: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
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Doesn't ISO-9000 allow up to 5% error? My ISO standard
from Geller says that it will do 5% or better.

It is odd though that your difference between X and Y is
so large. The Geller standard is calibrated in X and Y.

gary g.


At 12:33 PM 3/13/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Mar 13 19:13:55 2002



From: Richard Easingwood :      richard.easingwood-at-stonebow.otago.ac.nz
Date: Thu, 14 Mar 2002 14:20:00 +1300
Subject: Opinions on SEM cryo systems?

Contents Retrieved from Microscopy Listserver Archives
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Nancy,
It sounds to me like your FE is taking the average of the two errors,
which is NOT correct. I would be very persistent that they correct the
error. If you received this response while the FE was there on a service
visit, I would place a call the service office and demand that someone
return to perform the calibrations that should have been done in the first
place. Just my $.02 worth from someone who has been servicing SEM's for 20
+ years.

Gary M. Easton, President
Scanners Corporation
410.857.7633


----- Original Message -----
} From: "Nancy Zjaba" {nzjaba-at-alfalight.com}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 13, 2002 3:33 PM


Dear Microscopists,
We are considering purchasing a high resolution FE-SEM equipped with a high
resolution cryo prep & cryo transfer system. We are thinking of either
Gatan's Alto 2500 or the Baltec VCT 100.

We have two questions:
1) Does anybody have experiences they would like to share (positive or
negative experiences welcome!) regarding these two cryo systems.

2) Would either of these two systems (on a FE-SEM), in your opinion,
replace our dedicated freeze etch machine? Our present freeze etch device
is a Balzers BAF 300. Its old and we would dearly like to recover the space
occupied by this large instrument but are not sure whether the SEM cryo
systems we are considering will fully replace it.

Any thoughts garnered from experience would be gratefully received.
Opinions sent directly to me will be kept confidential.


Regards,


Richard Easingwood

Richard Easingwood
Otago Centre for Electron Microscopy
Department of Anatomy and Structural Biology
School of Medical Sciences, University of Otago
PO Box 913, Dunedin
NEW ZEALAND
Telephone: office: 0064 3 479 7301
Facsimile: 0064 3 479 7254
GSM: 0064 21 222 4759
mailto:richard.easingwood-at-stonebow.otago.ac.nz
Web site: http://www.otago.ac.nz/anatomy/emunit/








From daemon Wed Mar 13 19:40:25 2002



From: Paul Webster :      pwebster-at-hei.org
Date: Wed, 13 Mar 2002 17:35:19 -0800
Subject: Practical Course announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EMBO Practical Course on Electron Microscopy, Immunocytochemistry and
Stereology for Cell Biology at EMBL, Heidelberg, May 22 - June 01, 2002

This is a course about the production of thin sections of biological
material and their use in studying ultrastructure in the context of
molecular cell biology. The course will introduce the techniques of
cryosectioning, rapid freezing methods as an alternative to chemical
fixation, freeze substitution and resin embedding. It will instruct
participants in the sectioning of suitably prepared material and will then
concentrate on the use of these sections for localizing specific molecules
within cells. Ample time will be given to hands on practical work as well as
formal and informal discussion of available techniques for the localization
of subcellular molecules.

More details at http://www.embl-heidelberg.de/courses/ElectronMicroscopy02/

Regards,

Paul Webster.




Paul Webster, Ph.D.
Scientist II and Director
Ahmanson Center for Advanced EM & Imaging
House Ear Institute
2100 West 3rd Street
Los Angeles
CA 90057

pwebster-at-hei.org
p: 213 273 8026
f: 213 413 6739
http://www.hei.org/research/depts/aemi/aemi.htm





From daemon Thu Mar 14 04:51:34 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 14 Mar 2002 10:41:25 +0000
Subject: Re: Ask-A-Microscopist: How to make Microscope "slides for high School students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Loctite Glass Bond is available in 3ml tubes which are convenient
for schoolkids to handle. It is a glass-clear UV-curing adhesive,
used for bonding e.g. automobile mirrors to windscreens, which
can be used to mount all manner of *small* dry items like pollen
grains, diatoms, mosquito wings, butterfly wing scales, mineral
grains, microfossils, isolated plant cuticles, fibres etc. under a
coverslip

You don't need a UV source - daylight will do fine. Just place a
drop on the slide, arrange your specimens, cover with a coverslip
and leave on a window ledge for 15-30 minutes. Pollen etc. can be
collected and mounted in the field, the mounts hardening quickly in
full daylight, so that there is no risk of messy slippage of coverslips
during subsequent transport. The mount is permanent, and the
adhesive will not harden on fingers etc., only in situations where
oxygen is excluded. Clean up can be done with alcohol or soap
and water. Toxicity of the acrylic resin is no doubt finite but is
probably quite low.
Sources - auto spares dealers may stock this, otherwise try
Loctite Glass Bond RS Components Product Number 693-810 3ml
tube
http://www.rs-components.com/

A similar product is available from RS Components in larger
dispensers described as Loctite Engineering Adhesive

I have no commercial interest, but all donations gratefully received!

Best wishes
Chris

} } Question: I am a high school science teacher. I require either a
} } website or a protocol to have high school kids be able to make
} } permanent mount glass slides for the old fashion microscope in orcer
} } to create a permanent collection for the school. All I have found
} } so far is wet mount slides which is not the type I require. Any
} } suggestion would be helpful. Thanks.
} } Mike
} } michelbruneau-at-hotmail.com
} }
} } ---------------------------------------------------------------------------
Mike - -

If you're hoping for fixed, sectioned, stained mammalian tissues, that will
probably be beyond your students' capabilities. If you want whole mounts
of things like insect wings, diatoms, etc., that's possible. I suggest
that you get a copy of:
-----------------------
Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy; it's unequalled as a
basic reference for beginners. Almost half of the book is devoted to
simple preparation methods for biological specimens and descriptions (with
good illustrations) of commonly encountered organisms. Adult. RECOMMENDED
-----------------------
It will also tell you about your "old fashion microscope". The book
description is taken from the Project MICRO bibliography (URL below);
you'll find a lot of useful websites there too.

Where are you located in Canada? Perhaps we can find a member of the
Microscopy Society of Canada who can advise you.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html



------- End of forwarded message -------
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Thu Mar 14 04:55:44 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Thu, 14 Mar 2002 11:47:42 +0100
Subject: Re: Stereology Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A good starting place to find more information about stereology software
could be the website of the International Society for Stereology (ISS):

http://www.stereologysociety.org/

Best regards,

Peter

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

===============================================
Ken Bart wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Folks:
} Can anyone make a recommend software to make 3D
} reconstructions from serial TEM and LM images? Thanks for all
} suggestions!
}
} Ken
} --
}
} Kenneth M. Bart
} Director, Electron Microscopy Facility
} Hamilton College
} Clinton, New York 13323
}
} Phone:315-859-4715
} Fax: 315-859-4807
} email: kbart-at-hamilton.edu
} http://academics.hamilton.edu/biology/kbart/


From daemon Thu Mar 14 05:25:34 2002



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 14 Mar 2002 05:20:49 -0800
Subject: RE: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You've received a number of answers already, but there is a simple answer
to your problem and the service engineer's response. Manufacturer's
specifications for magnification are generally 'within 10%'. The
instruments are normally able to maintain 1 or 2%, assuming appropriate
conditions are met, but in setting their specifications the manufacturers
have to face the large dynamic range of the magnification (5 orders of
magnitude), accelerating voltage, condensor lens settings and working
distances. Couple that with the fact that their service engineers don't
carry NIST primary or secondary standards and they have little ability to
provide better calibration than you have received.

Your best chance would be to purchase and maintain your own NIST or derived
standard and either negotiate a contract to calibrate the instrument to a
certain percentage to that or use software based corrections in your image
processing software that you measure and document yourself. Of course, you
could also consider the services of a third party maintenance service that
may happily provide these services (all right, I happen to be one of those
third party services, but even if I weren't I would make this suggestion).

Seriously, if you want or need any traceability in magnification
calibration to any particular standard, you really have to maintain your
own standards. A service engineer travels many places in many ways making
the constant certification of any standards he carries impossible to
adequately certify. While the previous (current? I haven't checked to see
if NIST has introduced their next generation SEM mag calibration standard)
NIST standard essentially required recertification only if it were
subjected to re-polishing, standards organizations would also like some
documentation of the storage and condition of the standard beyond this.

I happen to carry both the NIST magnification and resolution standards, but
I still suggest to customers who have a need for tight specs to buy and
maintain their own standards. While I will provide ISO certification based
on these standards, these certs will include a statement of additional and
unknown uncertainties based on my inability to fully document the handling
and environmental conditions they may have been exposed to since
certification.

OK, that goes beyond most user's needs, but given the ongoing insidious
spread of ISO, it had to be stated. For most users, just knowing that the
magnification in each dimension is within a few percent is comforting.
Frankly, your field engineer is being lazy. Please feel free to show him
this response, or perhaps forward it to his service department. It may
have some effect, but probably not.

More modern instruments have less of an ability to adjust the calibration
for the full range of conditions, so a reasonable alternative is to define
a set of conditions that represent the area you are normally working
within. Establish a particular set of accelerating voltage, condensor lens
setting and working distance that you work at on average and request that
at that point, calibration be done within a certain percentage. Realize
also, that modern digital image capture systems add another problem. If
your system includes a photographic device, the calibration is probably
done to that image size, whether you use it or not. Since magnification
data is often not included in the digitally captured image, other than a
micron marker, and display or printing aspect ratios can vary, calibration
of digital images is a whole other mater.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, March 13, 2002 12:33 PM, Nancy Zjaba
[SMTP:nzjaba-at-alfalight.com] wrote:
}
} Hello, listers,
}
} I am using an FESEM (Hitachi S-4000). I've noticed a disparity in
} magnification calibration between the X and Y directions. I get about
1.5
} percent error in the Y direction, and almost 5 percent in the X
direction.
} Because the percentages still fall within 10 percent, the field service
} engineer believes that this is not a problem.
}
} What do you think? Thanks in advance for your input.
}
} Nancy Zjaba, Technical Staff
} Alfalight Inc.
} 1832 Wright Street
} Madison, WI 53704
} (608) 240-4875
} nzjaba-at-alfalight.com
}
}



From daemon Thu Mar 14 07:40:49 2002



From: Torsten.Fregin-at-zoologie.uni-hamburg.de
Date: Thu, 14 Mar 2002 07:30:51 -0600
Subject: Re: Stereology Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am also looking for such a solution, b/c right now I am cutting
lots of serial semithin
sections. So far I collected this information:

Some people at my department use regular CAD-Software or other 3D
rendering software
like "Alias Wavefront 3D studio tools", but that is a lot of work,
because the software was not
designed for such a task. As far as I know they redraw every single
picture by hand...
http://www.aliaswavefront.com/en/Home/homepage.shtml

Amira 2.3 is a software for 3D reconstruction of confocal images, but
with the new version
2.3 they integrated functionality for using the same functions with
single pictures, which can
be automatically (or manually) aligned in a stack. 14 day trial
download: www.amiravis.com

"AnalySIS 3.1 3D reconstruction" does the same, but was especially
designed for TEM and
LM. www.soft-imaging.net for information, but no trial software.

Personally I have not reconstructed a 3D model from serial sections
yet. I am not affiliated
with any of the mentioned brands, but recommend to buy Amira for our
confocal microscope.

:-) Torsten



}
} Hi Folks:
} Can anyone make a recommend software to make 3D
} reconstructions from serial TEM and LM images? Thanks for all
} suggestions!
}
} Ken
} --
}


Torsten Fregin

Universität Hamburg - Zoologisches Institut
Abt. Neurophysiologie
AG Wiese - Raum 413
Martin-Luther-King-Platz 3
20146 Hamburg, Germany
Telefon *49-(0)40-42838-3931
Fax *49-(0)40-42838-3937
eMail Torsten.Fregin-at-zoologie.uni-hamburg.de
oder TorstenFregin-at-gmx.de


From daemon Thu Mar 14 08:05:46 2002



From: Paul.Nolan-at-alcan.com
Date: Thu, 14 Mar 2002 08:59:33 -0500
Subject: Re: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On a similar note:
We discovered yesterday that our SEM magnification calibration is out by
about 25 % !!
We are working on recalibrating but according to our best estimate it has
been this way for about 3 weeks.
My question(s) is this.
How often do people check the magnification on their SEM's. How often do
you calibrate.
Like the temperature of my oven .. I always assume the magnification of my
SEM is pretty close to the stated value.
Anyway ..back to redoing the last 3 weeks of work !!

Cheers

Paul D. Nolan






Nancy Zjaba
{nzjaba-at-alfaligh To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
t.com} cc:
Subject: SEM magnification calibration
03/13/2002 03:33
PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello, listers,

I am using an FESEM (Hitachi S-4000). I've noticed a disparity in
magnification calibration between the X and Y directions. I get about 1.5
percent error in the Y direction, and almost 5 percent in the X direction.
Because the percentages still fall within 10 percent, the field service
engineer believes that this is not a problem.

What do you think? Thanks in advance for your input.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com








From daemon Thu Mar 14 08:31:40 2002



From: Daniele Spehner :      daniele.spehner-at-efs-alsace.fr
Date: Thu, 14 Mar 2002 15:24:20 +0100
Subject: Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Objet : liquid nitrogen


To all the cyomicroscopists arround,

I remember that a few months ago ( or years perhaps) there was a debate on
the list concerning burns with liquid nitrogen. Since I cannot find the
summary on my computer I address all of you the question : do you wear
gloves to work with and "in" liquid nitrogen ?
I do not because I was a student of H. Sitte and know by heart his safety
rules. I invited Keith Ryan a few years ago, and he too told us the rules
but nevertheless my boss does not agree with me and wants to force me to
wear gloves etc...
I am a member of the safety commettee in the lab and we have a meeting
next week.
My second inquiry is : Could you tell me about your experience with liquid
nitrogen or send the
web site where I can find the information? (as I remember there were some
funny stories on this list. In one somebody takes off all his clothes
running
away from a leaky, exploding container, and was not burned....) Thanks
} Daniele
}
}
} Veuillez noter qu'en cas de problème d'envoi d'e-mail vous pouvez aussi
} m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} If you have problem sending me an e-mail please try my other address as
} follows : daniele.spehner-at-efs-alsace.fr
}
} Danièle Spehner,
} Head of the EM Department
} INSERM EPI 99-08,
} Etablissement Français du Sang-Alsace,
} 10 rue Spielmann, 67065 STRASBOURG
} FRANCE

Veuillez noter qu'en cas de problème d'envoi d'e-mail vous pouvez aussi
m'adresser votre message à daniele.spehner-at-efs-alsace.fr
If you have problem sending me an e-mail please try my other address as
follows : daniele.spehner-at-efs-alsace.fr

Danièle Spehner,
INSERM EPI 99-08,
Etablissement Français du Sang-Alsace,
10 rue Spielmann, 67065 STRASBOURG
FRANCE



From daemon Thu Mar 14 09:44:50 2002



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Thu, 14 Mar 2002 09:33:02 -0600
Subject: RE: Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Insulating gloves (with gauntlets) should be worn, as should full-face
shields. The gloves should be over-sized, however, so that they can be flung
free if LN2 somehow gets inside. Snug gloves, especially those with
gauntlets, can entrap LN2 next to the skin, leading many to abandon the
practice.

The single biggest danger with LN2 (or other cryogenics, is insufficient air
exchange). It kills with alarming frequency, especially in enclosures like
tanks, "pits" or poorly-ventilated basement labs (where microscopists
frequently toil).

Jeffrey A Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439
(630) 252-5594
FAX: (630) 972-4438

} ----------
} From: Daniele Spehner
} Sent: Thursday, March 14, 2002 8:24 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Liquid nitrogen
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Objet : liquid nitrogen
}
}
} To all the cyomicroscopists arround,
}
} I remember that a few months ago ( or years perhaps) there was a debate
} on
} the list concerning burns with liquid nitrogen. Since I cannot find the
} summary on my computer I address all of you the question : do you wear
} gloves to work with and "in" liquid nitrogen ?
} I do not because I was a student of H. Sitte and know by heart his safety
} rules. I invited Keith Ryan a few years ago, and he too told us the rules
} but nevertheless my boss does not agree with me and wants to force me to
} wear gloves etc...
} I am a member of the safety commettee in the lab and we have a meeting
} next week.
} My second inquiry is : Could you tell me about your experience with
} liquid
} nitrogen or send the
} web site where I can find the information? (as I remember there were some
} funny stories on this list. In one somebody takes off all his clothes
} running
} away from a leaky, exploding container, and was not burned....) Thanks
} } Daniele
} }
} }
} } Veuillez noter qu'en cas de problème d'envoi d'e-mail vous pouvez aussi
} } m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} } If you have problem sending me an e-mail please try my other address as
} } follows : daniele.spehner-at-efs-alsace.fr
} }
} } Danièle Spehner,
} } Head of the EM Department
} } INSERM EPI 99-08,
} } Etablissement Français du Sang-Alsace,
} } 10 rue Spielmann, 67065 STRASBOURG
} } FRANCE
}
} Veuillez noter qu'en cas de problème d'envoi d'e-mail vous pouvez aussi
} m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} If you have problem sending me an e-mail please try my other address as
} follows : daniele.spehner-at-efs-alsace.fr
}
} Danièle Spehner,
} INSERM EPI 99-08,
} Etablissement Français du Sang-Alsace,
} 10 rue Spielmann, 67065 STRASBOURG
} FRANCE
}
}
}


From daemon Thu Mar 14 09:44:50 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 14 Mar 2002 08:36:55 -0700
Subject: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The suggestion with the digital processing is definitely a good one.
However, you may have to maintain a large calibration table, as the
calibration can change from one voltage to another, different working
distances, etc. If the software can't do that, you'd have to keep that
calibration table somewhere else on the computer.

For highest accuracy, some of our customers actually keep a calibration
sample on the sample holder. When they acquire images, they also acquire an
image of the calibration sample (they move there only by moving the stage as
to maintain identical conditions). Then they calibrate the image of the
calibration standard and transfer that calibration to the first image.
Sounds a bit cumbersome, but it gives them calibration independent of the
microscope parameters.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Wednesday, March 13, 2002 2:17 PM
To: 'Nancy Zjaba'; Microscopy (E-mail)


It's not going to get fixed unless you bitch about it. But do it in a nice
way so that they you know that it is an issue with you and be insistent. If
they can adjust it, they should do it during the next routine.

In the interim, you can make the calibration perfect with digital
processing. Just adjust the size in pixels appropriately in any of a number
of software programs, e.g. Photoshop, ThumbsPlus, etc. If you paste the
image into a word-processing or presentation program, you can also change
the aspect ratios and sizes to make them perfect.

BTW, you do have the tilt correction off, right? I don't know which
direction the Hitachi stage tilts, but if you have the correction on and no
tilt, you can get an error.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Nancy Zjaba [mailto:nzjaba-at-alfalight.com]
Sent: Wednesday, March 13, 2002 3:33 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Hello, listers,

I am using an FESEM (Hitachi S-4000). I've noticed a disparity in
magnification calibration between the X and Y directions. I get about 1.5
percent error in the Y direction, and almost 5 percent in the X direction.
Because the percentages still fall within 10 percent, the field service
engineer believes that this is not a problem.

What do you think? Thanks in advance for your input.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Thu Mar 14 10:42:38 2002



From: JHoffpa464-at-aol.com
Date: Thu, 14 Mar 2002 11:35:58 EST
Subject: quick question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


i need a quick answer to a quick question. our lab is considering a tissue processor. need to know what the users of processors think. only users please. any info would be helpful.
john


From daemon Thu Mar 14 12:22:55 2002



From: Jeannette Taylor :      jvtaylo-at-emory.edu
Date: Thu, 14 Mar 2002 13:17:45 -0500
Subject: immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is an inquiry to anyone with experience in immunocytochemistry.

I am preparing LR White embedded material for immunocytochemistry
studies and would appreciate some opinions on
the use of 5% or 10% hydrogen peroxide as an enchant. Is it too
aggressive or does it really improve the availability of antigenic
sites?

Thank you,

Jeannette Taylor
jvtaylo-at-emory.edu




From daemon Thu Mar 14 12:59:41 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 14 Mar 2002 12:53:12 -0600
Subject: Need a freeze substitution unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

Can anyone tell me if there is a freeze-sub unit, preferably with a high-pressure freezer associated with it, anywhere in the vicinity of central Missouri (like within a day's drive)? We're working on getting one, but we're not there, yet, and a client needs one pretty soon.

Thanks in advance.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Thu Mar 14 12:59:42 2002



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 3/14/02 9:33 AM
Subject: RE: Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi George (and everyone),
Yes, I received this info yesterday from AOCS Marketing via email. Since it only had to do with announcing "Courses" on feed analysis I took the liberty to post it on the Aglabs email list server. It should reach about 365 feed/fert/pest/chemists throughout North America. Hope it helps to drum up some new students.
Hope you all are doing well these daze,
Best Wishes
Mike

Mike Bucker
Consolidated Labs of Virginia
Richmond, Va

} } } "George Falb" {gfalb-at-buckeyenutrition.com} 03/14/02 01:26PM } } }
Have you received the e-mail information below regarding the 2002 Microscopy
short courses? Hopefully I didn't miss any of you. Please forward on to any
of your work associates or colleagues that may be interested. I will be
doing the same.

Thanks...George S. Falb


----- Original Message -----
} From: "AOCS" {marketingmm-at-aocs.org}
To: "George Falb" {gfalb-at-buckeyenutrition.com}
Sent: Tuesday, March 12, 2002 11:52 AM



In addition to Fortner's comments, while using LN2, do not use thin
latex (or similar) gloves. LN2 can quickly freeze these thin gloves,
holding the cold tightly against the skin, causing a more serious
burn.

As far as eschewing all protection, that is not a good idea.
Incidental or momentary contact is not particularly dangerous because
a layer of gas forms between the skin and the LN2, insulating the skin
from damage. However sustained contact will result in burns.

One more danger of LN2 and nudity...it upsets some sensitive types on
the List. Been there....been burned.

Chuck Butterick


______________________________ Reply Separator _________________________________


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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Insulating gloves (with gauntlets) should be worn, as should full-face
shields. The gloves should be over-sized, however, so that they can be flung
free if LN2 somehow gets inside. Snug gloves, especially those with
gauntlets, can entrap LN2 next to the skin, leading many to abandon the
practice.

The single biggest danger with LN2 (or other cryogenics, is insufficient air
exchange). It kills with alarming frequency, especially in enclosures like
tanks, "pits" or poorly-ventilated basement labs (where microscopists
frequently toil).

Jeffrey A Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439
(630) 252-5594
FAX: (630) 972-4438

} ----------
} From: Daniele Spehner
} Sent: Thursday, March 14, 2002 8:24 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Liquid nitrogen
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Objet : liquid nitrogen
}
}
} To all the cyomicroscopists arround,
}
} I remember that a few months ago ( or years perhaps) there was a debate
} on
} the list concerning burns with liquid nitrogen. Since I cannot find the
} summary on my computer I address all of you the question : do you wear
} gloves to work with and "in" liquid nitrogen ?
} I do not because I was a student of H. Sitte and know by heart his safety
} rules. I invited Keith Ryan a few years ago, and he too told us the rules
} but nevertheless my boss does not agree with me and wants to force me to
} wear gloves etc...
} I am a member of the safety commettee in the lab and we have a meeting
} next week.
} My second inquiry is : Could you tell me about your experience with
} liquid
} nitrogen or send the
} web site where I can find the information? (as I remember there were some
} funny stories on this list. In one somebody takes off all his clothes
} running
} away from a leaky, exploding container, and was not burned....) Thanks
} } Daniele
} }
} }
} } Veuillez noter qu'en cas de problème d'envoi d'e-mail vous pouvez aussi
} } m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} } If you have problem sending me an e-mail please try my other address as
} } follows : daniele.spehner-at-efs-alsace.fr
} }
} } Danièle Spehner,
} } Head of the EM Department
} } INSERM EPI 99-08,
} } Etablissement Français du Sang-Alsace,
} } 10 rue Spielmann, 67065 STRASBOURG
} } FRANCE
}
} Veuillez noter qu'en cas de problème d'envoi d'e-mail vous pouvez aussi
} m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} If you have problem sending me an e-mail please try my other address as
} follows : daniele.spehner-at-efs-alsace.fr
}
} Danièle Spehner,
} INSERM EPI 99-08,
} Etablissement Français du Sang-Alsace,
} 10 rue Spielmann, 67065 STRASBOURG
} FRANCE
}
}
}





From daemon Thu Mar 14 14:27:57 2002



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Thu, 14 Mar 2002 13:21:06 -0700
Subject: RE: Liquid nitrogen

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microscopy-at-sparc5.microscopy.com


Despite all the facts that are out there concerning the safe handling of LN i.e.. the film of oil on the skin is a
protectant in and of itself and clothing just absorbs the LN and thus could cause burns to the skin below....we still
use cryoprotective gloves, a full face shield and a cryo bib type apron to protect clothing.

Seems a little hard to convince the safety people that the protective gear we need when handling LN is none or no
clothing.


Harry Ekstrom
Materials Laboratory

*Phone: (602) 231-2744
?Fax: (602) 231-1547
*e-mail: harry.ekstrom-at-honeywell.com
{mailto:harry.ekstrom-at-honeywell.com}







From daemon Thu Mar 14 15:30:56 2002



From: LIU, JINGYUE [AG/1000] :      jingyue.liu-at-Monsanto.com
Date: Thu, 14 Mar 2002 15:25:04 -0600
Subject: Postdoctoral Position in Biological Microscopy-Monsanto Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
The following Postdoctoral Research Associate position is available
immediately at Monsanto Company in St. Louis, Missouri. Potential candidates
please respond on line at the Monsanto website provided below.
----------------------------------------------------------------------------
-------------------------------------------------------

Monsanto Company
Department: Research & Development
Title: Postdoctoral Research Associate
Req Number: mons-00000241
Location(s): St. Louis MO
Responsibilities:
A two-year postdoctoral fellow position is available immediately for
developing, implementing, and applying advanced microscopy techniques to
elucidating the ultrastructure of plants, seeds, weeds and other biological
systems. Additionally, the selected individual is responsible for developing
new methods to improve the sample preparation protocols of biological
systems. The selected candidate will interact with multifunctional groups of
scientists working on biotechnology projects.
Required Skills:
The position requires a Ph.D. in plant biology or a related field with
experience in advanced electron and light microscopy techniques. A strong
background and extensive experience in TEM, high-resolution cryo-SEM, and
confocal laser scanning microscopy techniques are essential. Experience with
gene transformation in plants is a strong plus. The following key
competencies are desired: highly motivated and interested in developing new
imaging technologies; good interpersonal, verbal and written communication
skills; innovative and seeking opportunity to improve existing techniques
and processes.
To respond to this job, access our website -at-: www.monsanto.com
Please be sure to select the proper source!
Monsanto values diversity and is an equal opportunity affirmative action
employer.



From daemon Thu Mar 14 15:46:06 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 14 Mar 2002 14:44:35 -0700
Subject: RE: Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


.. and make sure not to trap any LN2 in your shoes!!

mike



-----Original Message-----
} From: Fortner, Jeffrey A. [mailto:fortner-at-cmt.anl.gov]
Sent: Thursday, March 14, 2002 8:33 AM
To: 'Daniele Spehner'; microscopy-at-sparc5.microscopy.com (E-mail)


Insulating gloves (with gauntlets) should be worn, as should full-face
shields. The gloves should be over-sized, however, so that they can be flung
free if LN2 somehow gets inside. Snug gloves, especially those with
gauntlets, can entrap LN2 next to the skin, leading many to abandon the
practice.

The single biggest danger with LN2 (or other cryogenics, is insufficient air
exchange). It kills with alarming frequency, especially in enclosures like
tanks, "pits" or poorly-ventilated basement labs (where microscopists
frequently toil).

Jeffrey A Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439
(630) 252-5594
FAX: (630) 972-4438

} ----------
} From: Daniele Spehner
} Sent: Thursday, March 14, 2002 8:24 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Liquid nitrogen
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Objet : liquid nitrogen
}
}
} To all the cyomicroscopists arround,
}
} I remember that a few months ago ( or years perhaps) there was a debate
} on
} the list concerning burns with liquid nitrogen. Since I cannot find the
} summary on my computer I address all of you the question : do you wear
} gloves to work with and "in" liquid nitrogen ?
} I do not because I was a student of H. Sitte and know by heart his safety
} rules. I invited Keith Ryan a few years ago, and he too told us the rules
} but nevertheless my boss does not agree with me and wants to force me to
} wear gloves etc...
} I am a member of the safety commettee in the lab and we have a meeting
} next week.
} My second inquiry is : Could you tell me about your experience with
} liquid
} nitrogen or send the
} web site where I can find the information? (as I remember there were some
} funny stories on this list. In one somebody takes off all his clothes
} running
} away from a leaky, exploding container, and was not burned....) Thanks
} } Daniele
} }
} }
} } Veuillez noter qu'en cas de problème d'envoi d'e-mail vous pouvez aussi
} } m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} } If you have problem sending me an e-mail please try my other address as
} } follows : daniele.spehner-at-efs-alsace.fr
} }
} } Danièle Spehner,
} } Head of the EM Department
} } INSERM EPI 99-08,
} } Etablissement Français du Sang-Alsace,
} } 10 rue Spielmann, 67065 STRASBOURG
} } FRANCE
}
} Veuillez noter qu'en cas de problème d'envoi d'e-mail vous pouvez aussi
} m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} If you have problem sending me an e-mail please try my other address as
} follows : daniele.spehner-at-efs-alsace.fr
}
} Danièle Spehner,
} INSERM EPI 99-08,
} Etablissement Français du Sang-Alsace,
} 10 rue Spielmann, 67065 STRASBOURG
} FRANCE
}
}
}


From daemon Thu Mar 14 15:50:04 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 14 Mar 2002 16:29:52 -0500
Subject: Re: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That is one good reason for having standards such as ISO9000 and QS9000. We have the calibration requirements and magnification calibration performed when a routine is done. On a TEM, whenever the column is split it should be checked, and otherwise once or twice a year with service routines is sufficient. You do need a traceable standard if your lab is certified. Otherwise, you can get very good standards that are not traceable that are accurate for most work and relatively inexpensive.

An important point that is being missed, but that was pointed out in the original question is that both directions, X and Y, should be checked. This is very important for TEM diffraction patterns. Here the easy check is to take two ring patterns acquired back-to-back and rotate one 90 degrees with respect to the other and check that the patterns are still coincident. Otherwise you have to take the aspect ratio difference into account

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com
[mailto:"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com]
Sent: Thursday, March 14, 2002 9:00 AM
To: Microscopy-at-sparc5.microscopy.com



On a similar note:
We discovered yesterday that our SEM magnification calibration is out by
about 25 % !!
We are working on recalibrating but according to our best estimate it has
been this way for about 3 weeks.
My question(s) is this.
How often do people check the magnification on their SEM's. How often do
you calibrate.
Like the temperature of my oven .. I always assume the magnification of my
SEM is pretty close to the stated value.
Anyway ..back to redoing the last 3 weeks of work !!

Cheers

Paul D. Nolan






Nancy Zjaba
{nzjaba-at-alfaligh To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
t.com} cc:
Subject: SEM magnification calibration
03/13/2002 03:33
PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello, listers,

I am using an FESEM (Hitachi S-4000). I've noticed a disparity in
magnification calibration between the X and Y directions. I get about 1.5
percent error in the Y direction, and almost 5 percent in the X direction.
Because the percentages still fall within 10 percent, the field service
engineer believes that this is not a problem.

What do you think? Thanks in advance for your input.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com








From daemon Thu Mar 14 15:52:18 2002



From: Rotole, John A. :      John.Rotole-at-ispat.com
Date: Thu, 14 Mar 2002 15:42:18 -0600
Subject: Comparison of SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning Everyone,

I am comparing a series of SEM images of steel samples with surfaces
that vary from lightly to severely etched (pickled in acid). As the
severity of the etching increases, the number of exposed metal grains
observed increases. Continued etching shows surfaces with mild to
severe grain boundary attack. Can anyone recommend an effective way
to objectively compare a large series of such images? Is there a
basic text or are there any particular references that may apply?

Cheers,

John

John A. Rotole, Ph.D.
Project Engineer
Coatings & Surface Technology
Ispat Inland Product Research
3001 E. Columbus Drive
East Chicago, Indiana 46312
Tel: 219-399-6308
Fax: 219-399-6562


From daemon Thu Mar 14 15:52:24 2002



From: Rotole, John A. :      John.Rotole-at-ispat.com (by way of Blank)
Date: Thu, 14 Mar 2002 15:44:16 -0600
Subject: Comparison of SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning Everyone,

I am comparing a series of SEM images of steel samples with surfaces
that vary from lightly to severely etched (pickled in acid). As the
severity of the etching increases, the number of exposed metal grains
observed increases. Continued etching shows surfaces with mild to
severe grain boundary attack. Can anyone recommend an effective way
to objectively compare a large series of such images? Is there a
basic text or are there any particular references that may apply?

Cheers,

John

John A. Rotole, Ph.D.
Project Engineer
Coatings & Surface Technology
Ispat Inland Product Research
3001 E. Columbus Drive
East Chicago, Indiana 46312
Tel: 219-399-6308
Fax: 219-399-6562


From daemon Thu Mar 14 16:05:11 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Thu, 14 Mar 2002 16:57:56 -0800
Subject: Re: Need a freeze substitution unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Randy,
is atlanta too far?
try Rob Apakarian at Emory {rapkari-at-emory.edu}
Beth

} Can anyone tell me if there is a freeze-sub unit, preferably with a
} high-pressure freezer associated with it, anywhere in the vicinity of
} central Missouri (like within a day's drive)? We're working on getting
} one, but we're not there, yet, and a client needs one pretty soon.
}
} Thanks in advance.
}
} Randy


**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Thu Mar 14 16:08:55 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 14 Mar 2002 17:05:48 -0500
Subject: Re: immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is my understanding that, since LR White is hydrophilic and not highly
cross-linked, etching is not necessary. However, as anyone who has done
immuno will tell you, a lot depends on what you are looking for. I never had
much luck with LR White, but mainly because the morphology was not good
enough for what we were trying to do.

Jeannette Taylor wrote:

} This is an inquiry to anyone with experience in immunocytochemistry.
}
} I am preparing LR White embedded material for immunocytochemistry
} studies and would appreciate some opinions on
} the use of 5% or 10% hydrogen peroxide as an enchant. Is it too
} aggressive or does it really improve the availability of antigenic
} sites?
}
} Thank you,
}
} Jeannette Taylor
} jvtaylo-at-emory.edu

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Mar 14 16:36:29 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 14 Mar 2002 16:26:24 -0600
Subject: RE: Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Daniele,

It has been my experience that the dangers of LN2 are blown all out of
proportion. Have you ever seen a picture of the "OSHA Cowboy"? As the
previous posted stated, the biggest danger is asphyxiation.

I use gloves when handling something (especially the fill hose and cryo
valve) that has been chilled by LN2. To keep H&S happy, I use a face shield
when filling my detector from my 4LD flask. It is at eye level.

LN2 in limited quantities (as in a spatter) will near instantly blow itself
off bare skin. Like dripping water on a red hot stove, it almost instantly
flashes to vapor at the contact point leaving in a hurry. Sustained contact
is a totally different matter and should be avoided.

IMHO, The safest clothing for work with LN2 is none at all. This can
present some (other) problems in most labs. {g}

I don't have the address, but did once locate a study by Union Carbide which
indicated no corneal damage when a limited amount of LN2 was dripped into
rabbit's eyes.

I wish I had a copy of a picture showing the late Chuck Fiori pouring a gush
of LN2 (from a 4LD held over his head) into his open mouth. Chuck was a bit
more adventuresome than I! I would probably hiccup...

Most handling rules I have seen seem to have been written by someone who has
read the MSDS and has no practical experience. One of our company divisions
(to paraphrase) states you should use a rubber apron, gauntlet cryo gloves,
high-top boots, face shield, etc, etc. After all that the final sentence is
the "punch line" - ...and don't wear anything that can trap LN2 against your
body. Go figure...

The above are my personal opinions, not those of my employer.

Woody
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Objet : liquid nitrogen
}
}
} To all the cyomicroscopists arround,
}
} I remember that a few months ago ( or years perhaps) there
} was a debate on
} the list concerning burns with liquid nitrogen. Since I
} cannot find the
} summary on my computer I address all of you the question :
} do you wear
} gloves to work with and "in" liquid nitrogen ?
} I do not because I was a student of H. Sitte and know by
} heart his safety
} rules. I invited Keith Ryan a few years ago, and he too told
} us the rules
} but nevertheless my boss does not agree with me and wants to
} force me to
} wear gloves etc...
} I am a member of the safety commettee in the lab and we have
} a meeting
} next week.
} My second inquiry is : Could you tell me about your
} experience with liquid
} nitrogen or send the
} web site where I can find the information? (as I remember
} there were some
} funny stories on this list. In one somebody takes off all his clothes
} running
} away from a leaky, exploding container, and was not burned....) Thanks
} } Daniele
} }
} }
} } Veuillez noter qu'en cas de problème d'envoi d'e-mail vous
} pouvez aussi
} } m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} } If you have problem sending me an e-mail please try my
} other address as
} } follows : daniele.spehner-at-efs-alsace.fr
} }
} } Danièle Spehner,
} } Head of the EM Department
} } INSERM EPI 99-08,
} } Etablissement Français du Sang-Alsace,
} } 10 rue Spielmann, 67065 STRASBOURG
} } FRANCE
}
} Veuillez noter qu'en cas de problème d'envoi d'e-mail vous
} pouvez aussi
} m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} If you have problem sending me an e-mail please try my other
} address as
} follows : daniele.spehner-at-efs-alsace.fr
}
} Danièle Spehner,
} INSERM EPI 99-08,
} Etablissement Français du Sang-Alsace,
} 10 rue Spielmann, 67065 STRASBOURG
} FRANCE
}
}


From daemon Thu Mar 14 17:29:32 2002



From: Mike Jercinovic :      mjj-at-geo.umass.edu
Date: Thu, 14 Mar 2002 18:22:46 -0500
Subject: Post-Doc position - reminder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,
Just a reminder for those interested. We will begin evaluating
applications soon for the position outlined below, but there is still time
if you would like to apply...

thanks,
Mike Jercinovic


POST-DOC POSITION: MICROPROBE MONAZITE GEOCHRONOLOGY

The Department of Geosciences at the University of Massachusetts invites
applications for a Post-Doctoral Position in Geology. This two-year
position is specifically aimed at the rapidly emerging techniques of
electron microprobe analysis in geochronologic applications. UMass is
currently developing an optimized electron microprobe with Cameca, France
that is specifically designed for the exploration of techniques for age
mapping and dating of minerals (e.g. monazite, zircon) and trace element
analysis. This project includes optimization on virtually all fronts,
hardware, software, and technique development. One future direction will
involve synthesis and analysis of standards for calibration and background
measurement studies. The successful applicant will collaborate with UMass
Geosciences faculty (and associates) and with Cameca, and will be directly
involved with improvements and modifications to software, continued
evaluation of analytical techniques, synthesis and characterization of
standard materials, and application of the new techniques to geologic
problems. Applicants must have completed a Ph.D. in Geology, materials
science, or other physical science, with preference given to those with
significant experience in electron microprobe analysis, x-ray spectrometry,
materials microanalysis, and/or scientific programming.

Please send a letter of application, resume, and two reference letters to
Michael L. Williams, Dept. of Geosciences, University of Massachusetts, 611
North Pleasant Street, Amherst, MA 01003-9279. The University of
Massachusetts is an Equal-Opportunity Affirmative -Action Employer; women
and members of minority groups are encouraged to apply.

Review of applicants will begin March 15th; the position will remain open
until a successful candidate is identified.


****************
Michael J. Jercinovic
Assistant Professor
Department of Geosciences
University of Massachusetts
611 North Pleasant Street
Amherst, MA 01003-9297
E-Mail: mjj-at-geo.umass.edu
Phone: (413) 545-2431
http://www.geo.umass.edu/faculty/jercinovic.html

Electron Microprobe Laboratory
http://www.geo.umass.edu/probe/probe.html




From daemon Thu Mar 14 17:30:55 2002



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Thu, 14 Mar 2002 17:20:41 -0600
Subject: SEM mag calibration - thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who replied to my question. The new NIST-traceable sample is
due to arrive tomorrow, so we will proceed from there. The service engineer
will be working with me to try to get better agreement between the X and Y
directions, although I guess the pot adjustments are somewhat tricky.

I did have the sample in the scope untilted. One lister suggested having
the calibration sample in the SEM with the sample of interest to do the
calibration, take the measurement, and do the calibration again. But most
of my samples are cross sections. Anyone have a fancy holder to accommodate
a cross section at 90 degrees, plus a calibration sample? That could be
useful.

Thanks again for your help.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Thu Mar 14 19:26:14 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 14 Mar 2002 19:17:13 -0600
Subject: Color balancing & color printing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to locate a book on digital imaging/publishing and color
balancing/printing published recently by a MSA member. Hopefully,
someone will remember a presentation given a couple years ago at our
M&M meeting in Philadelphia. The speaker included a good discussion
on color balancing especially for publication and printing. I belive
the fellow was a faculty member (molecular biologist?) and had just
completed writing the book that was to go to press shortly. I
certainly remember the presentation but (blush-blush) not the guys
name. I do recall that it was not John Russ or John Mackenzie..

They say that the mind is the second thing to go...... Send ginseng.......

John B.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Mar 14 19:44:47 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Thu, 14 Mar 2002 20:39:06 -0500 (EST)
Subject: MonoCL2/3 on an S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all...

Could anyone who has an Oxford MonoCL2 or a Gatan MonoCl3 installed on an
Hitachi S-4700 FE-SEM contact me offline? It would be much appreciated.

Best,

Angela

-----------------------------------------
**Please note new department name**

Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------



From daemon Thu Mar 14 21:30:42 2002



From: jwagner-at-remc12.k12.mi.us (by way of Blank)
Date: Thu, 14 Mar 2002 22:43:38 -0600
Subject: Ask-A-Microscopist: Info needed about types of objectives for B&L

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

I run a daily performance check using a calibrated secondary standard for
measurements in both X and Y direction. The measured dimensions in both X
and Y as well as the ratio of the two must be within spec before the
instrument is used. I wrote a Standard Operating Procedure for this and the
same will become the standard for a daily performance check of our digital
cameras on optical microscopes and LSCM. What you experienced is the very
reason we have to do this!

Damian Neuberger


----- Original Message -----
} From: {"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 14, 2002 7:59 AM


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jwagner-at-remc12.k12.mi.us) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
March 14, 2002 at 21:09:46
---------------------------------------------------------------------------

Email: jwagner-at-remc12.k12.mi.us
Name: John Wagner

Organization: Just me

Education: Graduate College

Location: City, State, Country

Question: I used to spend hours as a child looking through my
microscope. Now I have children and I have just purched two
microscopes thru ebay. One is a Bausch & Lomb binocular Dynoptic, the
other is a Fisher student model. I am having a hard time finding
literature about the Dynoptic. Even though there is a head a base
that I can bid on to get the better light source for Koehler
lighting. I want to know more about the type of objectives...this
isn't an infinity corrected scope is it? Stuff like that, you know!

Hopefully,
John

---------------------------------------------------------------------------


From daemon Fri Mar 15 03:09:08 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 15 Mar 2002 09:04:49 +0000 (GMT Standard Time)
Subject: Re: RE: Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Daniele,

Although I would have to agree that the effect of
asphixiation is the most serious it is certainly not the
most common.

Problems with asphixiation can be dealt with by building
design, forced ventilation and environmental conditions
around the area.

Problems with burns are more common and are often caused by
over familiarity. Users are normally wary of liquid N2 the
first time they see it, lots of steam and boiling, but they
can then become cavalier in their handling. They find that
the occasional splash does not hurt so they don't worry
when they get several splashes until they find it trapped
in their sleeve or bracelet. They forget that the high
pressure jet of steam when filling from the main storage
dewar has a jet of N2 in the core, so they get burnt.

I am on our dept safety committee and I see the
accident/incident reports, probably averaging 1 every 2
years, from our ever changing base of some 60 N2 users.

I use cryo gloves to handle cold objects but not when
handling dewars or buckets. I tell people all the hazards
and try to teach them the real dangers - burns and
complacency: liquid N2 hurts so avoid it.

I would also like to cover another effect (I cannot spell
phenomenon). When filling some EDX or ACD dewars from room
temperature they can throw out a large amount of N2 shortly
after filling. This is caused by the N2 boiling on the
bottom of the dewar creating a N2 gas layer which
insulates, as the dewar gets colder there is less boiling
and a smaller gas insulation layer. When the liquid N2
finally touches the bottom of the dewar the boiling rate
increases and suddenly you get a lot of gas given off. If
the dewar is fairly full of liquid N2 then liquid is
ejected from the dewar with the gas. When filling from cold
only put a small amount of N2 in the dewar until it is
properly cooled. This will prevent you getting showered
with N2 a few minutes after filling the dewar.

Ron


} }
} } Objet : liquid nitrogen
} }
} }
} } To all the cyomicroscopists arround,
} }
} } I remember that a few months ago ( or years perhaps) there
} } was a debate on
} } the list concerning burns with liquid nitrogen. Since I
} } cannot find the
} } summary on my computer I address all of you the question :
} } do you wear
} } gloves to work with and "in" liquid nitrogen ?
} } I do not because I was a student of H. Sitte and know by
} } heart his safety
} } rules. I invited Keith Ryan a few years ago, and he too told
} } us the rules
} } but nevertheless my boss does not agree with me and wants to
} } force me to
} } wear gloves etc...
} } I am a member of the safety commettee in the lab and we have
} } a meeting
} } next week.
} } My second inquiry is : Could you tell me about your
} } experience with liquid
} } nitrogen or send the
} } web site where I can find the information? (as I remember
} } there were some
} } funny stories on this list. In one somebody takes off all his clothes
} } running
} } away from a leaky, exploding container, and was not burned....) Thanks
} } } Daniele
} } }
} } }
} } } Veuillez noter qu'en cas de problème d'envoi d'e-mail vous
} } pouvez aussi
} } } m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} } } If you have problem sending me an e-mail please try my
} } other address as
} } } follows : daniele.spehner-at-efs-alsace.fr
} } }
} } } Danièle Spehner,
} } } Head of the EM Department
} } } INSERM EPI 99-08,
} } } Etablissement Français du Sang-Alsace,
} } } 10 rue Spielmann, 67065 STRASBOURG
} } } FRANCE
} }
} } Veuillez noter qu'en cas de problème d'envoi d'e-mail vous
} } pouvez aussi
} } m'adresser votre message à daniele.spehner-at-efs-alsace.fr
} } If you have problem sending me an e-mail please try my other
} } address as
} } follows : daniele.spehner-at-efs-alsace.fr
} }
} } Danièle Spehner,
} } INSERM EPI 99-08,
} } Etablissement Français du Sang-Alsace,
} } 10 rue Spielmann, 67065 STRASBOURG
} } FRANCE
} }
} }
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri Mar 15 03:29:43 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Fri, 15 Mar 2002 10:22:02 +0100
Subject: Re: Stereology Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Someone who knows a lot about stereology and could probably help you out
on stereology software is Professor Hans Jørgen G. Gundersen from the
University of Aarhus in Denmark.

You can find more information on the website of the Stereological
Research Laboratory of the University of Aarhus:

http://www.health.au.dk/dept/stereol.htm

Best regards,

Peter

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/


From daemon Fri Mar 15 06:35:44 2002



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Fri, 15 Mar 2002 07:26:34 -0500
Subject: RE:Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The discussion seems to be focused around contact with the LN2, however, in
my experience one is more likely to be injured from handling the transfer
equipment. i.e. an extremely cold transfer line or a frozen valve handle.
My vote is to wear very loose fitting gauntlet style gloves.

} John A. Robson
} _____________________________________________________
}
} Boehringer Ingelheim Pharmaceuticals, Inc.
} Research and Development
} 900 Ridgebury Road / P. O. Box 368
} Ridgefield, CT 06877-0368
}
} phone: 203.798.5640
} fax: 203.798.5698
} email: jrobson-at-rdg.boehringer-ingelheim.com
}
}
}
}
} -----Original Message-----
} From: Ekstrom, Harry [SMTP:harry.ekstrom-at-honeywell.com]
} Sent: Thursday, March 14, 2002 3:21 PM
} To: 'Daniele Spehner'; microscopy-at-sparc5.microscopy.com
} Subject: RE:Liquid nitrogen
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Despite all the facts that are out there concerning the safe handling of
} LN i.e.. the film of oil on the skin is a
} protectant in and of itself and clothing just absorbs the LN and thus
} could cause burns to the skin below....we still
} use cryoprotective gloves, a full face shield and a cryo bib type apron to
} protect clothing.
}
} Seems a little hard to convince the safety people that the protective gear
} we need when handling LN is none or no
} clothing.
}
}
} Harry Ekstrom
} Materials Laboratory
}
} *Phone: (602) 231-2744
} ?Fax: (602) 231-1547
} *e-mail: harry.ekstrom-at-honeywell.com
} {mailto:harry.ekstrom-at-honeywell.com}
}
}
}
}
}
}



From daemon Fri Mar 15 07:36:12 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 15 Mar 2002 08:26:11 -0500
Subject: SEM X-Y Magnification?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Question.

1. How does the shape of the beam affect X and Y magnifications?
AND,
2. How about SED's or other detectors that are placed symmetrically
or asymmetrically with respect to the specimen and multi-image registration?

Regards,

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


From daemon Fri Mar 15 07:37:10 2002



From: Paul.Nolan-at-alcan.com
Date: Fri, 15 Mar 2002 08:31:58 -0500
Subject: Re: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Thanks Scott.
Parts of our lab are ISO certified but not our electron optics department.
I do have several (non traceable) standards that i used to find out that we
did indeed have a problem. Its just that it never occurred to me to think
about doing a check. (bad practice on my part i guess) I think it may be a
monthly check from now often
We do have our instrument calibrated twice a year when we have our routine
done.
This is the first time in my 15 years in EM that i have seen an SEM go that
far out of whack.
It may have been because we were fiddling around hooking up some new
peripherals.

PS If anyone happens to know who i am and know my equipment manufacturer
is, i must say that the service engineers are top notch and got me up and
running the next morning.

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



"Walck, Scott
D." To: "Microscopy (E-mail)" {microscopy-at-sparc5.microscopy.com}
{walck-at-ppg.com} cc:
Subject: RE: SEM magnification calibration AND TEM
03/14/2002 04:29
PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


That is one good reason for having standards such as ISO9000 and QS9000.
We have the calibration requirements and magnification calibration
performed when a routine is done. On a TEM, whenever the column is split
it should be checked, and otherwise once or twice a year with service
routines is sufficient. You do need a traceable standard if your lab is
certified. Otherwise, you can get very good standards that are not
traceable that are accurate for most work and relatively inexpensive.

An important point that is being missed, but that was pointed out in the
original question is that both directions, X and Y, should be checked.
This is very important for TEM diffraction patterns. Here the easy check
is to take two ring patterns acquired back-to-back and rotate one 90
degrees with respect to the other and check that the patterns are still
coincident. Otherwise you have to take the aspect ratio difference into
account

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com
[mailto:"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com]
Sent: Thursday, March 14, 2002 9:00 AM
To: Microscopy-at-sparc5.microscopy.com



On a similar note:
We discovered yesterday that our SEM magnification calibration is out by
about 25 % !!
We are working on recalibrating but according to our best estimate it has
been this way for about 3 weeks.
My question(s) is this.
How often do people check the magnification on their SEM's. How often do
you calibrate.
Like the temperature of my oven .. I always assume the magnification of my
SEM is pretty close to the stated value.
Anyway ..back to redoing the last 3 weeks of work !!

Cheers

Paul D. Nolan






Nancy Zjaba

{nzjaba-at-alfaligh To:
"'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}

t.com} cc:

Subject: SEM magnification
calibration
03/13/2002 03:33

PM







------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello, listers,

I am using an FESEM (Hitachi S-4000). I've noticed a disparity in
magnification calibration between the X and Y directions. I get about 1.5
percent error in the Y direction, and almost 5 percent in the X direction.
Because the percentages still fall within 10 percent, the field service
engineer believes that this is not a problem.

What do you think? Thanks in advance for your input.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com













From daemon Fri Mar 15 08:08:41 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 15 Mar 2002 08:59:47 -0500
Subject: RE: EM pics of Poliovirus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Christina,

From an old guy who only thinks he can find his way out of a cinched
paper bag,

The TCID 50 tells it all. Even the infectivity has to be measured
at the 50% error level. Is there an absolute morphological method that can
exceed even that poor level of precision? I don't know.

Answers to questions:

My best Answer to all 3 questions, IF they were mine. The
Salk institute is just down the road from you.

My answers.

1. negative stain
2. tritiated (or otherwise labeled) oligomers that will
bind exclusively with free, genomic RNA, and will NOT bind with complete
virus, encapsulated or not. Scintillation counting (my first choice) or EM
autoradiography (more statistics).
3. I suspect not, but they are very small. I would NOT
start such an investigation unless I first acquired a Beckman Airfuge with
an EM head, because you are demanding the capacity to count ALL particles in
a known volume.

I have NO relationship to the Airfuge except as a satisfied user, and the
Airfuge is the only part of my response that I didn't capture by
free-thinking.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


} ----------
} From: Christina Chan
} Sent: Monday, March 11, 2002 5:51 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: EM pics of Poliovirus
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I was given this address as a source for EM information, but I am not
} trained in EM _at all_...so please forgive what may be basic
} questions:
}
} 1. What is the procedure used for taking EM pictures of poliovirus
} (RNA virus)?
} 2. Is it possible to calibrate the number of genomes (capsulated RNA
} viruses as well as free floating RNA) in a known aliquot of virus
} dilution?
} 3. Is it difficult to recognize poliovirus RNA in its capsulated and
} free floating forms in an EM picture?
}
} Our lab is trying to find the number of genomes of poliovirus in a
} stock solution of 10^8 TCID50/1ml. Any help or advice I could get
} would be greatly appreciated!!
}
} Thanks,
} Christina Chan
} --
} LSRA - Lab Manager
} Maldonado Lab
} Pediatrics, Infectious Disease
} Stanford University Medical Center
} lab: (650) 736-1310
} cell: (650) 996-0098
}
}


From daemon Fri Mar 15 08:13:54 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Fri, 15 Mar 2002 09:08:38 -0500
Subject: Re[2]: Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


i am a firm believer that no gloves are better than any gloves the risk is to
great. while in dallas a not to bright tech wore the wrong gloves and was
badly burned. the liendefrost effect will protect from the momentary contact
with LN2. the key is to keep all contact to a minimum.
john


From daemon Fri Mar 15 08:45:18 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 15 Mar 2002 08:36:49 -0600
Subject: Liquid Nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Regarding liquid nitrogen safety, I do not use gloves (except when handling supercold transfer lines from the big dewars)for the same reasons that others have stated. Getting LN2 down inside a glove is no fun and the bulky cryo gloves make this clumsy tech even clumsier.

However, I have had two plastic funnels explode violently and scatter sharp plastic shards over quite a distance while transferring LN2 between dewars. I managed to locate metal funnels at a farm and home store, the only local source I was able to find, so that problem was solved. But I shudder to think of the hundreds of times I filled an eye level plastic funnel on the back of a Hitachi H500 to put nitrogen on the pumps.

Also, I nearly beaned myself one time through stupidity when I loosened the spigot on a 50L dewar without bleeding the pressure off first. The spigot popped out of the dewar like a huge champagne cork with surprising force, straight at my face. It was stopped about an inch from my nose by the safety cable attached to the dewar handle.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Fri Mar 15 08:49:56 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 15 Mar 2002 09:41:56 -0500
Subject: Re: Color balancing & color printing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} I am trying to locate a book on digital imaging/publishing and color
} balancing/printing published recently by a MSA member. Hopefully,
} someone will remember a presentation given a couple years ago at our
} M&M meeting in Philadelphia. The speaker included a good discussion
} on color balancing especially for publication and printing. I belive
} the fellow was a faculty member (molecular biologist?) and had just
} completed writing the book that was to go to press shortly. I
} certainly remember the presentation but (blush-blush) not the guys
} name. I do recall that it was not John Russ or John Mackenzie..
}
} They say that the mind is the second thing to go...... Send ginseng.......
}
} John B.
}
} --
} #############################################################
Hi John,
Was is M. Joseph Costello and/or John J. Lemasters? (UNC-Chapel
Hill) I have a73 page handout from them entitled "The Digital
Darkroom" that I got at the Philadelphia meeting session of the same
name.
Lee
--
Lee Cohen-Gould
Electron & Optical Microscopy Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207


From daemon Fri Mar 15 08:54:35 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 15 Mar 2002 09:34:09 -0500
Subject: SEM X-Y Magnification?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1. The shape of the beam affects the astigmatism that you see in the image, not the magnification. Of course, you should have a perfectly conical beam after correcting for astigmatism.

2. Detectors can have an affect on the position of the beam. They can shift the beam when they are inserted of parameters are changed. This is particularly noticeable at lower beam energies. As an example, if you change the bias on your E-T detector while operating at low voltage, you will see the image shift. This is because the beam is shifted on your sample. Images in the SEM are created from the signal created at the position where the beam hits the sample, therefore, all of your images from different detectors will be in registration if you take them at the same time or under the same conditions. That is why you do not turn off the bias voltage on the E-T detector when you use another detector such as a backscatter or in-lens detector. Because the shift is small, these shifts will not have any affect on the magnification.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
Sent: Friday, March 15, 2002 8:26 AM
To: 'List-Microscopy'


Question.

1. How does the shape of the beam affect X and Y magnifications?
AND,
2. How about SED's or other detectors that are placed symmetrically
or asymmetrically with respect to the specimen and multi-image registration?

Regards,

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


From daemon Fri Mar 15 08:59:38 2002



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Fri, 15 Mar 2002 09:53:52 -0500
Subject: Question on materials sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,
I have a question regarding sample prep for silicon IC's. Has anyone out
there used an automated polisher for flat, planar surface polishing? I've
been asked to look into them, but i would like to get a broad cross section
of users input to go along with my own conclusions (when i get some). For
any of you who have used them, I would like to know what make/model, ease of
use, and does it do what it is supposed to do? Thank you all very much for
your time
Nick


From daemon Fri Mar 15 09:09:24 2002



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Fri, 15 Mar 2002 10:03:29 -0500
Subject: May 3 Workshop"Digital Image Capture and Management" LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


New York Microscopical Society

30 North Mountain Avenue

Montclair, NJ 07042








Bernard Friedman


Memorial Workshop





Digital Image Capture and Management in Microscopy

May 3, 2002


A course on Digital imaging in light microscopy which will cover the
following topics:


Optical Limitations in Light Microscopy...Photographic Imaging Strategies...
Digital Imaging Strategies...Selection of Digital Capture (Camera vs.
Scanner)...

Image Processing of Captured Images...Image File Formats...Printing
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WHEN: Friday, May 3, 2002, from 9 A.M. to 5 P.M.

WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043

COST: $330 for N.Y.M.S. members, $360 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

HOW: Register using the form below. Limited to the first 12 registrants.

Return form with a check to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ
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FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 e-mail
donoleary-at-att.net

PLEASE POST

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N.Y.M.S. Member_________________ ($330) Non-Member__________($360)

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e-mail________________________________________

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New York Microscopical Society
6 Chittenden Road
Fair Lawn, NJ 07410
(201) 797-8849






From daemon Fri Mar 15 10:16:59 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Fri, 15 Mar 2002 10:50:40 -0500
Subject: Re: immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeannette,
I find 5% sodium meta-periodate to work well on reduced osmium
postfixed samples in LR White for immuno.
Mike D.

Jeannette Taylor wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} This is an inquiry to anyone with experience in immunocytochemistry.
}
} I am preparing LR White embedded material for immunocytochemistry
} studies and would appreciate some opinions on
} the use of 5% or 10% hydrogen peroxide as an enchant. Is it too
} aggressive or does it really improve the availability of antigenic
} sites?
}
} Thank you,
}
} Jeannette Taylor
} jvtaylo-at-emory.edu



From daemon Fri Mar 15 10:51:16 2002



From: Nathan Haese :      nathanhaese-at-yahoo.com
Date: Fri, 15 Mar 2002 08:37:45 -0800 (PST)
Subject: RE: Liquid Nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Harry,

If you are successful in persuading your safety
management to your way of thinking about clothing,
will you be posting invitations to your company safety
meetings where the proper safety attire will be
demonstrated?

With anticipation,

Nathan Haese
Lafayette, CA

************* Your message ******************

"Despite all the facts that are out there concerning
the safe handling of LN ... Seems a little hard to
convince the safety people that the protective gear we
need when handling LN is none or no clothing."


Harry Ekstrom
Materials Laboratory

*Phone: (602) 231-2744
?Fax: (602) 231-1547


__________________________________________________
Do You Yahoo!?
Yahoo! Sports - live college hoops coverage
http://sports.yahoo.com/


From daemon Fri Mar 15 11:09:45 2002



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Fri, 15 Mar 2002 11:03:28 -0600
Subject: RE: Liquid Nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



This posting makes an important point: The use of a face shield is
primarily to protect oneself from flying debris- small explosions or violent
fracture of embrittled hoses, funnels, etc. are a common hazard. LN2
splashed in the face would probably be harmless (unless a jet under
pressure).

I would insist that gloves are still a good idea. Sometimes one is
confronted with unexpected problems- an iced-over valve, super-cold
components that are somehow in the way, etc. Without those gloves handy, it
may be difficult to respond properly when things go wrong. Safety equipment
also serves to remind us that we are doing hazardous operation, and its
proper use limits hazards and (our own and our employers') liabilities.

-Another $.02

Jeffrey A Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439
(630) 252-5594
FAX: (630) 972-4438


} Regarding liquid nitrogen safety, I do not use gloves (except when
} handling supercold transfer lines from the big dewars)for the same reasons
} that others have stated. Getting LN2 down inside a glove is no fun and
} the bulky cryo gloves make this clumsy tech even clumsier.
}
} However, I have had two plastic funnels explode violently and scatter
} sharp plastic shards over quite a distance while transferring LN2 between
} dewars. I managed to locate metal funnels at a farm and home store, the
} only local source I was able to find, so that problem was solved. But I
} shudder to think of the hundreds of times I filled an eye level plastic
} funnel on the back of a Hitachi H500 to put nitrogen on the pumps.
}
} Also, I nearly beaned myself one time through stupidity when I loosened
} the spigot on a 50L dewar without bleeding the pressure off first. The
} spigot popped out of the dewar like a huge champagne cork with surprising
} force, straight at my face. It was stopped about an inch from my nose by
} the safety cable attached to the dewar handle.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}


From daemon Fri Mar 15 12:07:28 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 15 Mar 2002 11:04:21 -0700
Subject: SEM X-Y Magnification?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'll try to answer that.

1) In an SEM the magnification is determined (in X) by the ratio of the
length scanned to the length displayed on the video unit. Similarly in Y.
The shape of the beam is irrelevant. It is, of course, relevant for
resolution. If your beam is not circular, you get different resolution in X
and Y (also known as astigmatism).

2) Detector position: as the image on the screen is correlated with the beam
position and not the detector position, it has no effect on magnification.
You should be able to place your detector anywhere in the chamber (maybe not
directly in the beam path :-) and it has no effect on the magnification.
Same for multiple detectors.

mike



} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
Sent: Friday, March 15, 2002 6:26 AM
To: 'List-Microscopy'


Question.

1. How does the shape of the beam affect X and Y magnifications?
AND,
2. How about SED's or other detectors that are placed symmetrically
or asymmetrically with respect to the specimen and multi-image registration?

Regards,

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


From daemon Fri Mar 15 12:09:06 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Fri, 15 Mar 2002 12:04:15 -0600
Subject: RE: recommendations for measuring space between grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers: Many thanks for all the input I got on this
topic. I now have many
more options to pursue. The Listserver is a great knowledge
base and my thanks to
Nestor for keeping it running.


--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Fri Mar 15 12:20:43 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 15 Mar 2002 11:18:31 -0700
Subject: Re[2]: Liquid nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would think that, at least for the users of LN2 in the US, there are OSHA
regulations regarding what can be used and what not. Although the OSHA
regulations may be a bit ambiguous, your employers or yourself may be held
responsible if you act against those regulations and something happens. I
went to the OSHA site (www.OSHA.gov) and looked up Nitrogen. Most of it
dealt with gaseous N2, but there was this chapter:

PERSONAL PROTECTIVE EQUIPMENT

Workers should use appropriate personal protective clothing and equipment
that must be carefully selected, used, and maintained to be effective in
preventing skin contact with liquid nitrogen. The selection of the
appropriate personal protective equipment (PPE) (e.g., gloves, sleeves,
encapsulating suits) should be based on the extent of the worker's potential
exposure to liquid nitrogen. There are no published reports on the
resistance of various materials to permeation by liquid nitrogen.

As I said, it is ambiguous, but it clearly says, that "protective clothing
.. must worn to prevent skin contact"

The full text is under:

http://www.osha.gov/SLTC/healthguidelines/nitrogen/recognition.html

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: John Hoffpauir [mailto:John.Hoffpauir-at-mail.tju.edu]
Sent: Friday, March 15, 2002 7:09 AM
To: cbutte-at-ameripol.com
Cc: daniele.spehner-at-efs-alsace.fr; fortner-at-cmt.anl.gov;
microscopy-at-sparc5.microscopy.com


i am a firm believer that no gloves are better than any gloves the risk is
to
great. while in dallas a not to bright tech wore the wrong gloves and was
badly burned. the liendefrost effect will protect from the momentary contact
with LN2. the key is to keep all contact to a minimum.
john


From daemon Fri Mar 15 12:58:03 2002



From: LIU, JINGYUE [AG/1000] :      jingyue.liu-at-Monsanto.com
Date: Fri, 15 Mar 2002 12:52:31 -0600
Subject: Postdoctoral Position in Biological Microscopy at Monsanto Compa

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

The following Postdoctoral Research Associate position is available
immediately at Monsanto Company in St. Louis, Missouri. Potential candidates
please respond on line at the Monsanto website provided below.
----------------------------------------------------------------------

Monsanto Company
Department: Research & Development
Title: Postdoctoral Research Associate
Req Number: mons-00000241
Location(s): St. Louis MO

Responsibilities:
A two-year postdoctoral fellow position is available immediately for
developing, implementing, and applying advanced microscopy techniques to
elucidating the ultrastructure of plants, seeds, weeds and other
biologicalsystems. Additionally, the selected individual is responsible for
developing new methods to improve the sample preparation protocols of
biological systems. The selected candidate will interact with
multifunctional groups of scientists working on biotechnology projects.

Required Skills:
The position requires a Ph.D. in plant biology or a related field with
experience in advanced electron and light microscopy techniques. A strong
background and extensive experience in TEM, high-resolution cryo-SEM, and
confocal laser scanning microscopy techniques are essential. Experience with
gene transformation in plants is a strong plus. The following key
competencies are desired: highly motivated and interested in developing new
imaging technologies; good interpersonal, verbal and written communication
skills; innovative and seeking opportunity to improve existing techniques
and processes.

To respond to this job, access our website -at-: www.monsanto.com
Please be sure to select the proper source!

Monsanto values diversity and is an equal opportunity affirmative action
employer.


From daemon Fri Mar 15 13:09:57 2002



From: Helvey, Marc :      Marc.Helvey-at-vlsistd.com
Date: Fri, 15 Mar 2002 11:03:32 -0800
Subject: Re: SEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Feel free to review the product specifications for our new 100 nm pitch SEM
Calibration Standard at:

http://www.vlsistandards.com/products/so_nanolattice.asp?cid=3&sid=78

Benefits include:
100 nm pitch
Highly Uniform
Small Line Edge Roughness
High Image contrast
Wafer or die formats
Cleanable
NIST-Traceable
Low defectivity
{1% accuracy (95% confidence)

Click on "Applications Notes" for more details.

Regards -

Marc Helvey
Strategic Accounts Manager
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
Phone: (408) 428-1800, ext. 108
FAX: (408) 428-9555
E-Mail: marc.helvey-at-vlsistd.com {mailto:marc.helvey-at-vlsistd.com}
Internet: http://www.vlsistandards.com




-----Original Message-----
} From: "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com
[mailto:"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com]
Sent: Thursday, March 14, 2002 6:00 AM
To: Microscopy-at-sparc5.microscopy.com



On a similar note:
We discovered yesterday that our SEM magnification calibration is out by
about 25 % !!
We are working on recalibrating but according to our best estimate it has
been this way for about 3 weeks.
My question(s) is this.
How often do people check the magnification on their SEM's. How often do
you calibrate.
Like the temperature of my oven .. I always assume the magnification of my
SEM is pretty close to the stated value.
Anyway ..back to redoing the last 3 weeks of work !!

Cheers

Paul D. Nolan







Nancy Zjaba

{nzjaba-at-alfaligh To:
"'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}

t.com} cc:

Subject: SEM magnification
calibration
03/13/2002 03:33

PM









------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello, listers,

I am using an FESEM (Hitachi S-4000). I've noticed a disparity in
magnification calibration between the X and Y directions. I get about 1.5
percent error in the Y direction, and almost 5 percent in the X direction.
Because the percentages still fall within 10 percent, the field service
engineer believes that this is not a problem.

What do you think? Thanks in advance for your input.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com








From daemon Fri Mar 15 14:07:45 2002



From: Nicholas W. M. Ritchie :      nritchie-at-aspexllc.com
Date: Fri, 15 Mar 2002 15:01:31 -0500
Subject: Re: SEM X-Y Magnification?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1

Frederick,
On an SEM, the magnification is determined by the strength
of the scan coil deflection. The deflection is a function
of the scan coil geometry and the current driving the scan
coil. Since the scan coil geometry is essentially fixed but
not necessarily perfectly balanced between the x and y axes,
an SEM will typically have potentiometers to fine tune the
scan coil current. You should be careful when adjusting
these potentiometers. Since many (?most/?all) SEMs
compensate for the rotation of the image due to the
objective lens, you must be careful that you differentiate
between a rotation compensated image and a non-compensated
image. If you try to adjust the relative magnification on a
rotation compensated image you will probably find the
process very confusing. The x and y axes on the image will
not correspond directly to the x and y scan coils. More
than likely the image axes will be a linear combination of
the x and y coils.
The shape of the beam does not affect the x and y
magnifications just the sharpness of the image. Likewise,
the placement of the detectors would not effect the x and y
magnifications. If you observe a shift in your image when
you select different detectors it is more than likely this
results from fields generated by the detectors interacting
with the beam. For example, the secondary detector uses a
many kV potential to pull electrons from the sample. Turn
this potential on and off while observing a back-scatter
image and you may notice an image shift.

Sincerely,

Nicholas W M Ritchie

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~\ Nicholas W. M. Ritchie, Ph.D. /~
| Aspex Instruments |
\ _ 175 Sheffield Drive _ /
/ Delmont, PA 15626 \
| (724) 468-5400 |
~/ nritchie-at-aspexllc.com \~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

-----BEGIN PGP SIGNATURE-----
Version: PGP Personal Privacy 6.5.8

iQA/AwUBPJJRtqy9TCUIpFOoEQLFxQCfbYLa+OtZB4woGKaFRzcviawGW3kAn10w
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=CzV+
-----END PGP SIGNATURE-----
3/15/2002 8:26:11 AM, "Monson, Frederick C." {fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From daemon Fri Mar 15 15:16:11 2002



From: Ken Bart :      kbart-at-hamilton.edu
Date: Fri, 15 Mar 2002 16:11:12 -0500
Subject: Stereology help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All:

Thanks to everyone who provided suggestions for stereology
software! I appreciate to help.

Ken
--


Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323

Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu
http://academics.hamilton.edu/biology/kbart/


From daemon Fri Mar 15 15:32:40 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Fri, 15 Mar 2002 16:25:06 -0500
Subject: Liquid Nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,

Your story reminded me of a concern our safety dept. discovered many years
ago. We had two valves in series on our 160 liter LN2 tanks. We never
thought of the remote possibility of getting liquid trapped between these
valves.
I can only imagine the sound that would make.

Russ Gillmeister
Xerox
~~~~~~~~~~~~~


-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Friday, March 15, 2002 9:37 AM
To: microscopy-at-sparc5.microscopy.com


Regarding liquid nitrogen safety, I do not use gloves (except when handling
supercold transfer lines from the big dewars)for the same reasons that
others have stated. Getting LN2 down inside a glove is no fun and the bulky
cryo gloves make this clumsy tech even clumsier.

However, I have had two plastic funnels explode violently and scatter sharp
plastic shards over quite a distance while transferring LN2 between dewars.
I managed to locate metal funnels at a farm and home store, the only local
source I was able to find, so that problem was solved. But I shudder to
think of the hundreds of times I filled an eye level plastic funnel on the
back of a Hitachi H500 to put nitrogen on the pumps.

Also, I nearly beaned myself one time through stupidity when I loosened the
spigot on a 50L dewar without bleeding the pressure off first. The spigot
popped out of the dewar like a huge champagne cork with surprising force,
straight at my face. It was stopped about an inch from my nose by the
safety cable attached to the dewar handle.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Fri Mar 15 16:56:54 2002



From: Blackwood, Andrew :      ablackwood-at-2spi.com
Date: Fri, 15 Mar 2002 17:42:10 -0500
Subject: Handling Liquid Nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Blackwood, Andrew * EMC.Ver #3.1a ] --

15 March 2002

Hi Listers:

After several decades of handling liquid nitrogen in what I then thought was
a safe manner (although I'm horrified to think of some of the things that
I've done over the years before I knew better), I recently had a chance to
learn more than I ever wanted to know about liquid nitrogen handling in
general and gloves in particular as I did an evaluation of additions to the
product line of SPI Supplies. The result of the effort can be found on the
following URL

http://www.2spi.com/catalog/supp/cryo-gloves.html

and the pages linked to it. There are a couple of points that I think add to
the overall discussion of liquid nitrogen handling safety:

1. There are a variety of designs of gloves, and one shouldn't draw general
conclusions about gloves from experience with one design. For example,
people who might pour liquid nitrogen into the gloves can use those with
wrist bands or those that extend up the arm.

2. Modern materials offer better protection against liquid nitrogen than
leather, and either offers infinitely more protection than bare skin. Given
the same spill, it takes longer for you to feel the effect of the liquid
nitrogen with the man-made materials than with leather. Yes, you can safely
pour small amounts of liquid nitrogen on your skin; the vapor blanket due to
the high vapor pressure of nitrogen at its boiling point will protect you
**if** you do not get any on your clothes or rings or watches or anything
else that will transmit heat (or in this case cold). You can get severely
burned if rings or clothes are cooled to a low temperature, as several
listers have pointed out.

3. Safety equipment is designed to protect against accidents, not to enable
you to do things that you shouldn't do. Sticking your hand into liquid
nitrogen, no matter what you're wearing, is a bad idea.

4. The material from which modern protective gloves are made offers
excellent protection against liquid nitrogen. Seams in that material (and a
glove has several seams in it) are another matter. While all gloves made
from the modern materials can be called "waterproof" because the material
from which they are made is waterproof, for the best protection, gloves are
available with a continuous internal layer which prevents liquid penetration
through the seams.

5. Gloves offer protection against things that might have been cooled by the
flowing stream of liquid nitrogen, like valves and hoses. A couple of weeks
ago, I caught myself about to shut off a valve that someone else had opened.
Why should I be wearing gloves; I was just walking down the hall.

6. Gloves can protect you from cryoburns, which can do a lot of damage to
you. No glove, however, will protect you against the hazard of asphyxiation,
which can kill you.

Disclaimer: I am the Laboratory Director of Structure Probe, Inc. which is
the parent company of SPI Supplies. We have an obvious interest in promoting
the use of the safety equipment which we sell, but we have an even greater
interest in keeping our customers, and those of our competitors, alive, well
and whole.

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
P.O. Box 656
West Chester, PA 19381-0656
Ph: 1 610 436 5400
FAX: 1 610 436 5755
e-mail: ablackwood-at-2spi.com
WWW: http://www.2spi.com


-------- REPLY, Original message follows --------

} Date: Thursday, 14-Mar-02 03:24 PM
}
} From: Daniele Spehner \ Internet: (daniele.spehner-at-efs-alsace.
fr)
} To: MICROSCOPY BB \ Internet:
} (microscopy-at-sparc5.microscopy.com)
}
} Subject: Liquid nitrogen
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To

-------- REPLY, End of original message --------



From daemon Fri Mar 15 19:07:46 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 15 Mar 2002 20:03:24 -0500
Subject: RE: Liquid Nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To All;

We use LN2 that is plumbed to our lab. from a large tank outside of our
building. The tank is several thousand gallons when full. There are no
cryogenic pumps between the tank and our hose in the lab. It is merely the
gas prssure derived thru a heat exchanger outside that pushes the LN2 thru
the line. That pressure can be as high as 150 psi. at times so I have to
agree with Jeffrey that there are considerations other than "burns." At
LN2 temperature, many things get very brittle and prone to fracture, as
described by Jeff.

It is better to be safe than to fill out all the paperwork and insurance
forms, not to mention the visit from "The Safety Guy."

Regards,

Peter Tomic
Anadigics, inc.

-----Original Message-----
} From: Fortner, Jeffrey A. [mailto:fortner-at-cmt.anl.gov]
Sent: Friday, March 15, 2002 12:03 PM
To: microscopy-at-sparc5.microscopy.com



This posting makes an important point: The use of a face shield is
primarily to protect oneself from flying debris- small explosions or violent
fracture of embrittled hoses, funnels, etc. are a common hazard. LN2
splashed in the face would probably be harmless (unless a jet under
pressure).

I would insist that gloves are still a good idea. Sometimes one is
confronted with unexpected problems- an iced-over valve, super-cold
components that are somehow in the way, etc. Without those gloves handy, it
may be difficult to respond properly when things go wrong. Safety equipment
also serves to remind us that we are doing hazardous operation, and its
proper use limits hazards and (our own and our employers') liabilities.

-Another $.02

Jeffrey A Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439
(630) 252-5594
FAX: (630) 972-4438


} Regarding liquid nitrogen safety, I do not use gloves (except when
} handling supercold transfer lines from the big dewars)for the same reasons
} that others have stated. Getting LN2 down inside a glove is no fun and
} the bulky cryo gloves make this clumsy tech even clumsier.
}
} However, I have had two plastic funnels explode violently and scatter
} sharp plastic shards over quite a distance while transferring LN2 between
} dewars. I managed to locate metal funnels at a farm and home store, the
} only local source I was able to find, so that problem was solved. But I
} shudder to think of the hundreds of times I filled an eye level plastic
} funnel on the back of a Hitachi H500 to put nitrogen on the pumps.
}
} Also, I nearly beaned myself one time through stupidity when I loosened
} the spigot on a 50L dewar without bleeding the pressure off first. The
} spigot popped out of the dewar like a huge champagne cork with surprising
} force, straight at my face. It was stopped about an inch from my nose by
} the safety cable attached to the dewar handle.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}


From daemon Sat Mar 16 04:53:21 2002



From: Allen Sampson :      ars-at-sem.com
Date: Sat, 16 Mar 2002 04:47:15 -0800
Subject: RE: SEM X-Y Magnification?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Answer (maybe)

1. The shape of the beam is a basically a result of the irregularities in
the generation and shaping of the beam within the gun and column and are
termed astigmatism (to be more correct, a collection of aberrations that we
collectively refer to as astigmatism). However, your second question also
points to a form of astigmatism that is caused by irregularities in the
electrical fields within the sample chamber. The same sample chamber
electrical field irregularities can cause additional complications to the
compensations for x and y magnifications.

2. All secondary electron detectors, and most modern BSE detectors,
introduce into the sample chamber, in close proximity to the beam, high
voltage electrical fields. SE detectors can vary widely, but most are
asymmetrical to the beam. Most use a voltage of around +10,000 Volts on
the scintillator face to provide increased efficiency of scintillation.
Most, but not all, are further surrounded by a screen that is maintained
at around +300 Volts. This two stage voltage not only provides for
efficient collection of secondary electrons, but also helps to shield the
beam from the influence of the higher voltage fields from the scintillator
surface. Any electrical field gradient within the sample chamber will
affect the position of the beam as well as the resultant shape of the beam
itself. These fields are a problem primarily when the accelerating voltage
changes, as their effect will be in proportion to the energy of the
electrons in the beam. Due to the beam energy narrowing effects of greater
condenser currents, the astigmatic effects of these electrical fields can
be reduced, but their effects on beam position will remain.

As always, in these complex instruments, the resultant image is a balance
of many influences that may, or may not, be optimized for a particular
situation. My prior point about the number of compensations available to
service engineers in various instruments relates to this point in
particular. If adequate adjustments are available for the compensation of
the accelerating voltage, these magnification related effects can also be
canceled out. However, modern instruments are moving away from the
multitude of adjustments available previously. In doing so, the
manufacturers are limiting the ultimate precision available over the full
range of operating conditions of their instruments. Doing so also reduces
the training required for their service staff, so to their bottom line it
is attractive; and in the more production oriented usage of today may be
acceptable.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


}
} Question.
}
} 1. How does the shape of the beam affect X and Y magnifications?
} AND,
} 2. How about SED's or other detectors that are placed symmetrically
} or asymmetrically with respect to the specimen and multi-image
registration?
}
} Regards,
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} West Chester University
} West Chester, Pennsylvania, USA, 19383
} 610-738-0437
} fmonson-at-wcupa.edu
}



From daemon Sat Mar 16 06:50:13 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 16 Mar 2002 07:42:19 -0500
Subject: Re: SEM X-Y Magnification?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fred,
In an SEM neither the shape of the beam nor the detectors have any
effect on magnification. However, because of the double deflection scan
system used by most SEMs, working distance can have a considerable
effect on the X to Y ratio if the "knees" aren't set correctly.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Monson, Frederick C. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Sun Mar 17 10:56:09 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Sun, 17 Mar 2002 10:44:23 -0600
Subject: for John Bozzola

Contents Retrieved from Microscopy Listserver Archives
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John,

I just got my emails to you bounced back after 3 days with "permanent
fatal errors". This was copy/pasting in your email address from your
message.
Please contact me.

Apologies to the list.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Mon Mar 18 01:16:15 2002



From: =?iso-8859-2?Q?Krzysztof_Jan_H=FCbner?= :      hubner-at-IOd.krakow.pl
Date: Mon, 18 Mar 2002 07:40:55 +0100
Subject: Conference "Clean Steel"

Contents Retrieved from Microscopy Listserver Archives
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Good morning

Information about conference "CLEAN STEEL" 10-12 June 2002 Balatonfüred,
Hungary

you can found on the http://www.ombkenet.hu/

best regards

chris



From daemon Mon Mar 18 10:06:52 2002



From: Dee Breger :      micro-at-ldeo.columbia.edu (by way of MicroscopyListserver)
Date: Mon, 18 Mar 2002 09:54:14 -0600
Subject: used SEM prep equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,

I have some used SEM sample prep equipment to pass along to a new home.
The following units were all purchased in 1983; the first three were
refurbished by the sales/service rep just before being replaced in late
2001 and all were working fine as of that time:

1. Denton 502 floor model vacuum evaporator with large diff pump,
configured with one post for carbon rods and two posts (one high, one low)
for carbon fiber or metal wire (or baskets), rotating/tilting stage

2. Denton Desk-1 sputter coater fitted with (still-useable) Au/Pd target

3. Denton DCP Critical Point Dryer
4. Kevex horizontally-mounted Si(Li) detector with rotating window turret,
used on a Cambridge 250 Mark 2 SEM. Stored dry, still working fine when
boxed in late 2001.

Please contact me offline if you're interested.
Thanks,
Dee



***************************************************************
Please do not publicly post any of my correspondence without permission

Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 845/365-8640
F: 845/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)


From daemon Mon Mar 18 11:03:52 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 18 Mar 2002 11:47:06 -0500
Subject: Re: X, Y and Z magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Even graduate work 50 yards uphill from Goldstein and Joy didn't give me
access to the etherized esoterica of their engineer's understanding of these
matters many years ago. After all these years, I finally got it. Thanks to
all participants, and those kinder souls who might have felt the urge to
'slap me upside the head' with my ignorance.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu


From daemon Mon Mar 18 13:56:26 2002



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Mon, 18 Mar 2002 14:47:46 -0500
Subject: Kevex 4505 pulse processor documentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I'm trying to set up an old Kevex 4505 pulse processor for use on a
pin-diode x-ray detector. (New NIM bin pulse processors seem to run
~$2k.) Does anyone have the documentation on this unit? I would like to
know how to set up the various adjustments so that we can run the output
into an Ortec 490A SCA.

Thanks,
Henk Colijn


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Mon Mar 18 16:06:52 2002



From: Cathy Davis :      cathydavis-at-attbi.com
Date: Mon, 18 Mar 2002 13:59:18 -0800
Subject: Software Info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mac/PC Softare packages (Digital Imaging and Crystallography). I need
suggested resources to do research for a final project at school on what
is available, what do they do, differences between, and applications
for. Any lists of companies that make the software that I could contact
or literature that relates to this subject matter would be greatly
appreciated.

Thanks,
Cathy



From daemon Mon Mar 18 20:06:57 2002



From: Brendan Griffin :      bjg-at-cmm.uwa.edu.au
Date: Tue, 19 Mar 2002 09:05:40 +0800
Subject: pattern/image matching software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

we are about to undertake a materials characterisation project where
we will want to match internal structures - could anyone recommend
some simple image matching software eg fingerprint recognition,
preferably for MAC but PC is OK

I have web-searched and many options appear present - hence the
request for an experienced-based comment


thank you

Brendan
--




Brendan J. Griffin
Acting Director & Assoc. Professor in Electron Microscopy
Centre for Microscopy and Microanalysis
The University of Western Australia
First floor, Physics Building,
35 Stirling Highway
CRAWLEY, WA, AUSTRALIA 6009
ph 61-8-9380-2739
fax 61-8-9380-1087
mobile 0409-104-096
bjg-at-cmm.uwa.edu.au
http://cmm.uwa.edu.au/


From daemon Mon Mar 18 21:30:51 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 18 Mar 2002 22:19:50 -0500
Subject: Cryo gloves for LN2 handling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

There have been a number of postings referring to gloves to be worn when
handling liquid nitrogen.

I would like to point out that there are any number of gloves that might
pass as being suitable for this application, and they vary in terms of

a) length (mid-arm, up to elbow, up to shoulder) and
b) structure (some now are available with an inner bladder for extra
protection)

I understand the arguments against wearing any gloves. I see them as being
analogous to the arguments put forth for not wearing seat belts. However, a
glove with this extra liner (bladder) is clearly more protective than one
without such a liner.

I don't know if this is over-kill or not but if there are such concerns,
then by all means, one should be specifying a glove with a bladder, and this
is the style we describe as being "100% waterproof". The ones that do not
have this extra bladder, and the type that are now mainly used in EM labs,
do not have this extra bladder protection.

Additional information about these gloves can be found on URL http://www.
2spi.com/catalog/supp/cryo-gloves.html

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================


From daemon Tue Mar 19 02:15:02 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Tue, 19 Mar 2002 09:03:20 +0100
Subject: How to remove a Be Xray tube window ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi

Sorry to all, my question is not on a typicall topic of this list, but I
have often seen that a lot of us like to do tinkering.

A collegue want to know if it is possible to remove a Be window from an
old broken X-ray tube. He want to re-use it as window for diffraction
cameras and he has a dozen broken tubes. Yes I know, Be, BeO etc. is very
very toxic, we spoke about that a few weeks ago on the list. And he knows
it too.

So, does some know what kind of glue, soldering, etc is used to fix these
windows and what kind of solvant could be used to disolve this glue (or
all other possible way), without danger for the one who do it, and without
dammage for the window.

Thanks to all

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Tue Mar 19 09:17:58 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Tue, 19 Mar 2002 10:08:16 -0500
Subject: Hg Bulbs vs Finger Prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is it o.k., to remove (student) finger prints from a Hg bulb (for a Epi-
illuminator lamp) with a solvent (EtOH or Acetone) and a lint free cloth?

The bulb has NOT yet been installed or subjected to current/heat (i.e. the
finger prints haven't been burned on to the glass).

I'd rather not simply dispose of the new bulb (though diposing of the
student who can't be bothered to read is a possibility).



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Tue Mar 19 12:35:41 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 19 Mar 2002 10:19:46 -0800 (PST)
Subject: Re: Hg Bulbs vs Finger Prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have used lens paper and EtOH or lens cleaner with good results.

Bob
U of Washington
Seattle

On Tue, 19 Mar 2002, Richard Edelmann wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is it o.k., to remove (student) finger prints from a Hg bulb (for a Epi-
} illuminator lamp) with a solvent (EtOH or Acetone) and a lint free cloth?
}
} The bulb has NOT yet been installed or subjected to current/heat (i.e. the
} finger prints haven't been burned on to the glass).
}
} I'd rather not simply dispose of the new bulb (though diposing of the
} student who can't be bothered to read is a possibility).
}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}



From daemon Tue Mar 19 12:39:25 2002



From: Tamara Howard :      thoward-at-unm.edu
Date: Tue, 19 Mar 2002 11:33:51 -0700 (MST)
Subject: Re: Hg Bulbs vs Finger Prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Should be OK - didn't (or don't) some manufacturers send the new bulbs
with a solvent based, lint-free towlette? I'd not use much force when
wiping, but it should be fine.

Tamara


On Tue, 19 Mar 2002, Richard Edelmann wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is it o.k., to remove (student) finger prints from a Hg bulb (for a Epi-
} illuminator lamp) with a solvent (EtOH or Acetone) and a lint free cloth?
}
} The bulb has NOT yet been installed or subjected to current/heat (i.e. the
} finger prints haven't been burned on to the glass).
}
} I'd rather not simply dispose of the new bulb (though diposing of the
} student who can't be bothered to read is a possibility).
}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Tue Mar 19 14:58:41 2002



From: Alexander Black :      alexander.black-at-nuigalway.ie
Date: Tue, 19 Mar 2002 12:13:48 +0000
Subject: Microscopy in Ireland

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listmembers,
First of all, a very belated happy St. Patrick's Day (March 17th) to
you
all.
Secondly, as (I hope) that at least one thought about Ireland or things
Irish have passed through many people's minds this weekend, why not
consider taking a trip over this summer, and participating in the
Microscopical Society of Ireland's annual symposium.
It is intended to take place this August 28, 29 & 30th in the National
University of Ireland, Galway (http://www.nuigalway.ie) and is a
relatively informal forum for anything involving microscopes &
research. It would certainly be excellent to see some overseas
visitors, and to hear what you are doing in your labs.
In fact, it might be a nice way to sneak in a holiday and a conference!

See what you'll miss if you don't
attend...(http://www.irishholidays.com/ggtest.shtml)

A more formal announcement will be made next month, following the launch

of the society's website.

Sincerely,

Alexander Black
President,
The Microscopical Society of Ireland



From daemon Tue Mar 19 21:32:22 2002



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Wed, 20 Mar 2002 14:23:35 +1100
Subject: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I just have to buy camera for taking pictures of beetles directly or
throughs stereomicroscope Zeiss Jena Technoval.

If I follow properly all discussions on this listserver the most used camera
in Nikon Coolpix 995, isn't it?

Thank you very much for your kind reply

Keep care and be of good cheer

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
(title) 84 duke of Siebenlügner

websites:
http://www.coleoptera.org. and
http://www.egroups.com/group/coleoptera

University of Sydney
The Wentworth Bldg., B 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ICQ: 13610107

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).




From daemon Wed Mar 20 07:11:02 2002



From: Cynthia Shannon :      cshannon-at-nctimes.net (by way of
Date: Wed, 20 Mar 2002 06:58:57 -0600
Subject: Used manual specimen trimmer &/or tissue rotator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a used manual specimen trimmer &/or a tissue rotator
that they no longer need?
Thank you.
Cindy Shannon
{mailto:cshannon-at-nctimes.net} cshannon-at-nctimes.net


From daemon Wed Mar 20 07:14:16 2002



From: Jim Bentley :      bentleyj-at-ornl.gov (by way of MicroscopyListserver)
Date: Wed, 20 Mar 2002 07:06:21 -0600
Subject: RMS-MSA travel scholarship announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


MSA-RMS Travel Scholarship Award 2002

Through a cooperative agreement between the Microscopy Society of
America (MSA) and the Royal Microscopical Society (RMS), a travel
scholarship is available to a graduate student or post-doctoral
research assistant to attend a meeting or course organized by the
RMS. Details of such events can be found at http://www.rms.org.uk.
MSA will award up to $1000 towards travel and accommodation. RMS will
cover the registration costs and partial accommodation costs. The
applicant must be a member of MSA in good standing at the time the
application is submitted and at the time of the meeting or course.

For 2002, it is envisaged that the MicroScience 2002 Conference to be
held July 9-11 in London (May 1 abstract deadline) will be the
primary focus, although other RMS events will be considered.
Applications for the award should consist of:

1. Applicants full name, address, email address, and details of
academic and/or professional status;
2. A statement of up to 500 words outlining why the applicant wants
to attend a named conference or course;
3. A letter from the applicant's faculty advisor/supervisor
confirming the status of the applicant and detailing any
supplementary financial support; and
4. If required for a conference presentation, an appropriately
prepared abstract. This abstract will need to be submitted in
sufficient time and to have sufficient scientific content to be
accepted into the official program of the conference.
Applications should be sent to Dr. J. Bentley, MSA Awards Committee
Chair, Oak Ridge National Laboratory, Bethel Valley Road, PO Box
2008, Oak Ridge, TN 37831-6064 (email: bentleyj-at-ornl.gov) to arrive
no later than April 10, 2002. The awardee is expected to be notified
by April 17.

--
Jim Bentley
Microscopy, Microanalysis, Microstructures Group
Metals and Ceramics Division
Bldg. 4500-S, MS-6136
Oak Ridge National Laboratory
PO Box 2008
Oak Ridge, TN 37831-6136, USA

Tel: (865)574-5067 Fax: (865)241-3650 bentleyj-at-ornl.gov
express mail use "1 Bethel Valley Rd" instead of PO Box.
** Note the new group name, mail-stop, fax, and area code **


From daemon Wed Mar 20 08:04:51 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Wed, 20 Mar 2002 08:58:19 -0500
Subject: Thanks RE: Hg Bulbs vs Finger Prints

Contents Retrieved from Microscopy Listserver Archives
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Thanks for all the folks who replied!

The answer is "Yes" Clean the bulb. The Na in the finger prints invades
the quartz causing a lower melting point weakening the the bulb (theoretically).
AND becareful not to scratch the bulb when cleaning.



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Wed Mar 20 08:39:27 2002



From: Greg Strout :      gstrout-at-ou.edu
Date: Wed, 20 Mar 2002 08:31:14 -0600
Subject: Help with Film Scanners

Contents Retrieved from Microscopy Listserver Archives
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I know that on and off there have been substantial discussions about
film scanning on the list serve. We have the opportunity to purchase a
scanner and have looked at the most commonly mentioned models; Nikon
Coolscan, Agfa Duoscan etc. However, we have also come across the
Dimage Scan Multi Pro from Minolta and were wondering if anyone had any
experience/comments about this machine.

SCANNER READING RES. OPTICAL DENSITY A/D
CONVERSION
Dimage Scan Multi Pro 4,800 dpi 4.8 dynamic
range 16 bit
*also claims to have optional mutiformat size negative holder that will
accept TEM films

We have compared specs and of course prices for these machines.
All else being equal clearly the Dimage is the way to go.
Of course all else is not equal.

Does anyone have experience with these products?
Is there a reason not to go with the Dimage?

Comments from vendors welcome.
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
==================================================================




From daemon Wed Mar 20 08:59:49 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 20 Mar 2002 09:42:38 -0500
Subject: fluorescence in clay

Contents Retrieved from Microscopy Listserver Archives
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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have an investigator who wants to visualize microorganisms in soil via fluorescence (using acroline orange) but needs to to prevent dispursal of the clay particles when staining. The clay disperses in contact with water but not in other solvents. Is it possible to use acroline orange, or similar stain, in ETOH, MEOH, acetone or othe non-polar solvent?
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Wed Mar 20 09:03:41 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 20 Mar 2002 08:54:29 -0600
Subject: Hitachi H600 available

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Dear listers,

We are getting rid of a Hitachi H600 in great working condition, in order to save money on service contracts. If interested, please contact me off-list for details. I will also post details on Nestor's surplus equipment list at the MSA website.

Thanks,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Wed Mar 20 11:14:39 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 20 Mar 2002 12:02:31 -0500
Subject: RE: Hg Bulbs vs Finger Prints

Contents Retrieved from Microscopy Listserver Archives
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Richard,

I have never installed a Hg bulb without first 'cleaning' it with 50%
ethanol in 2x processed water. I use halves of the lint-free cloth I have
for the EM's to clean and then dry. When I change a bulb, I blow out the
housing with my trusty little vacuum, before installing the new one. Not
training or advice. Just an independent, personal approach.

Then again, I used to keep ethyl ether in my refrigerator, dip my fingers in
xylene to retrieve slides, dissect with no gloves, and clean up after I used
someone else's space. What do I know.

Fred Monson

P.S.1 A Dean once demanded that I remove a sign on my office door that
stated, "If I am reading, I AM working!"

P.S.2. Someone really ran into my car this morning, and I am still feeling
somewhat silly, though not a lot different from yesterday!


} ----------
} From: Richard Edelmann
} Reply To: edelmare-at-muohio.edu
} Sent: Tuesday, March 19, 2002 10:08 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Hg Bulbs vs Finger Prints
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is it o.k., to remove (student) finger prints from a Hg bulb (for a
} Epi-
} illuminator lamp) with a solvent (EtOH or Acetone) and a lint free cloth?
}
} The bulb has NOT yet been installed or subjected to current/heat
} (i.e. the
} finger prints haven't been burned on to the glass).
}
} I'd rather not simply dispose of the new bulb (though diposing of
} the
} student who can't be bothered to read is a possibility).
}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}


From daemon Wed Mar 20 11:24:23 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 20 Mar 2002 12:15:09 -0500
Subject: RE: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Your Grace*,

While the Coolpix 995 is an easy and ubiquitous choice, the
$1,500.00 price (for camera, adapter, and remote from US Nikon microscope
vendors) should be less now but isn't, though the pieces can be purchased
separately at lower price and greater difficulty. You might want to look at
the newer Coolpix 5000 which is currently available under the $1,000(USD)
mark - not including remote or adapter but has been mentioned on the list of
late.

I cannot speak to the issue of quality of the CCD's as I am only
just into the matter myself.

Yours Sincerely,

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson-at-wcupa.edu

*(Ref:
http://www.parliament.the-stationery-office.co.uk/pa/ld/ldinfo/ldadds.htm)


} ----------
} From: Vr. Richard Bejsak-Colloredo-Mansfeld
} Reply To: Vr. Richard Bejsak-Colloredo-Mansfeld
} Sent: Tuesday, March 19, 2002 10:23 PM
} To: microscopy-at-sparc5.microscopy.com
} Cc: coleoptera-at-yahoogroups.com
} Subject: Digital camera
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I just have to buy camera for taking pictures of beetles directly or
} throughs stereomicroscope Zeiss Jena Technoval.
}
} If I follow properly all discussions on this listserver the most used
} camera
} in Nikon Coolpix 995, isn't it?
}
} Thank you very much for your kind reply
}
} Keep care and be of good cheer
}
} Regards
}
} (name) Vratislav Richard Eugene Maria John Baptist
} (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
} (title) 84 duke of Siebenlügner
}
} websites:
} http://www.coleoptera.org. and
} http://www.egroups.com/group/coleoptera
}
} University of Sydney
} The Wentworth Bldg., B 62
} NSW 2006
} AUSTRALIA
} phone : +61 414 540 465
} email: vratislav-at-bigfoot.com
} ICQ: 13610107
}
} Only after the last tree has been cut down,
} only after the last river has been poisoned,
} only after the last fish has been caught,
} only then will you find that money can not be eaten.'
} CREE INDIAN PROPHECY.
}
} Incoming mail is certified Virus Free.
} Checked by AVG anti-virus system (http://www.grisoft.com).
}
}
}
}


From daemon Wed Mar 20 12:24:40 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 20 Mar 2002 13:18:23 -0500
Subject: Re: Help with Film Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This site has a thorough review of the the scanner in question. I don't know
if the Minolta scanner does 31/4 x 4 EM film.

http://www.kenrockwell.com/minolta/mp.htm

Geoff

Greg Strout wrote:

} I know that on and off there have been substantial discussions about
} film scanning on the list serve. We have the opportunity to purchase a
} scanner and have looked at the most commonly mentioned models; Nikon
} Coolscan, Agfa Duoscan etc. However, we have also come across the
} Dimage Scan Multi Pro from Minolta and were wondering if anyone had any
} experience/comments about this machine.
}
} SCANNER READING RES. OPTICAL DENSITY A/D
} CONVERSION
} Dimage Scan Multi Pro 4,800 dpi 4.8 dynamic
} range 16 bit
} *also claims to have optional mutiformat size negative holder that will
} accept TEM films
}
} We have compared specs and of course prices for these machines.
} All else being equal clearly the Dimage is the way to go.
} Of course all else is not equal.
}
} Does anyone have experience with these products?
} Is there a reason not to go with the Dimage?
}
} Comments from vendors welcome.
} --
} ==================================================================
} Greg Strout
} Electron Microscopist, University of Oklahoma
} WWW Virtual Library for Microscopy:
} http://www.ou.edu/research/electron/www-vl/
} e-mail: gstrout-at-ou.edu
} ==================================================================

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Mar 20 13:17:25 2002



From: AP Alves de Matos :      apamatos-at-oninet.pt
Date: Wed, 20 Mar 2002 19:06:16 -0000
Subject: TEM: Fixation of yeast

Contents Retrieved from Microscopy Listserver Archives
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Dear Collegues

I work with specimens from vertebrate tissues with good results. A collegue
asked me to make some observations in yeast (Saccharomyces cervisiae) and I
used my default techniques.

Cellular detail, particularlu membranes was very poorly preserved.
Can anyone give indications on fixation and embedding protocols suitable for
yeast, and point special problems of this material?

Thank you all in advance


---------------
Prof. Dr. A.P Alves de Matos
apamatos-at-oninet.pt
Biologist
Anatomia Patológica, Hospital Curry Cabral (Tel/Fax 217924268)
Departamento de Biomateriais, Faculdade de Medicina Dentária (217922652)
Lisboa



From daemon Wed Mar 20 13:17:43 2002



From: Cathy Davis :      cathydavis-at-attbi.com
Date: Wed, 20 Mar 2002 11:12:09 -0800
Subject: Software Info Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone,
I just wanted to thank all of you who sent information to me regarding
software packages. It was the first time I used the list server and was
glad I did. Thank you all very much!

Cathy Davis



From daemon Wed Mar 20 13:19:49 2002



From: George Laing :      scisales-at-ngscorp.com
Date: Wed, 20 Mar 2002 14:07:21 -0500
Subject: RE: Help with Film Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I saw the Minolta Dimage Scan Multi Pro at PMA recently. Nice scanner,
nice specs. On the plus side...
4800 dpi, 4.8 dmax, both IEEE1394(Firewire) and SCSI interfaces, multi
sample scanning.

For TEM films be aware of the following...
4800x4800 dpi res is for 35mm film. On 120/220 (and TEM) films, optical
resolution is 3200 x 4800(still very good).

3.25 x 4" TEM films will not fit in the scanner without being cut. The
maximum scan area is 56.8 x 83.8mm.
There is a "Multi Format Set" available which allows you to create "custom"
carriers, but still TEM negs will have to be trimmed.

Minolta (and Nikon with the Coolscan 8000ED) advertise that Electron
Microscope films can be scanned but neither will accept 3.25 x 4 film
without trimming.

I have not tried the Minolta myself so I cannot comment on using it. The
Agfa Duoscan T2500 and Duoscan Hi-D were our most popular scanners for TEM
films. Agfa has discontinued them but the Microtek Artixscan 2500 and
Artixscan 1100 are their mechanical twins.

George

George Laing
National Graphic Supply
v:(800) 223-7130 x3109
f:(800) 832-2205
email: scisales-at-ngscorp.com


However, we have also come across the
Dimage Scan Multi Pro from Minolta and were wondering if anyone had
anyexperience/comments about this machine.



From daemon Wed Mar 20 13:26:19 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 20 Mar 2002 14:20:18 -0500
Subject: Correction:fluorescence in clay

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The stain was acridine orange not acroline orange.
Sorry!

I have an investigator who wants to visualize microorganisms in soil via fluorescence (using acroline orange) but needs to to prevent dispursal of the clay particles when staining. The clay disperses in contact with water but not in other solvents. Is it possible to use acroline orange, or similar stain, in ETOH, MEOH, acetone or othe non-polar solvent?
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Wed Mar 20 13:59:27 2002



From: Todd Clason :      clason-at-u.washington.edu
Date: Wed, 20 Mar 2002 11:52:46 -0800
Subject: Re: TEM: Fixation of yeast

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I've had good luck with yeast when following the protocols set by Dr. Robin
Wright:

Wright, R. 2000 Transmission Electron Microscopy of Yeast. Microscopy
Research and Technique. 51:496-510.

Cheers,

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Todd A. Clason, Imaging Coordinator
Department of Zoology
University of Washington
Box 351800
Seattle, WA 98195-1800

A087 PAB
clason-at-u.washington.edu
Tel: (206) 685-1519
Fax: (206) 543-3041
http://depts.washington.edu/zooweb/confocal_index.html
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Collegues
}
} I work with specimens from vertebrate tissues with good results. A collegue
} asked me to make some observations in yeast (Saccharomyces cervisiae) and I
} used my default techniques.
}
} Cellular detail, particularlu membranes was very poorly preserved.
} Can anyone give indications on fixation and embedding protocols suitable for
} yeast, and point special problems of this material?
}
} Thank you all in advance
}
}
} ---------------
} Prof. Dr. A.P Alves de Matos
} apamatos-at-oninet.pt
} Biologist
} Anatomia Patológica, Hospital Curry Cabral (Tel/Fax 217924268)
} Departamento de Biomateriais, Faculdade de Medicina Dentária (217922652)
} Lisboa
}
}



From daemon Wed Mar 20 14:25:21 2002



From: Louis Kerr :      lkerr-at-mbl.edu
Date: Wed, 20 Mar 2002 15:27:20 -0500
Subject: Cryo LM

Contents Retrieved from Microscopy Listserver Archives
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We are preparing for an upcoming course and have a specific request to
demonstrate "cryo" light microscopy. An instructor in the would like to
image living oocytes on an inverted light microscope and watch ice
crystal formation in the culture dish medium. I would like to know
whether anyone can suggest a commercial system that would accomplish
this technique.

Thanks,
Louie

--
Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITE:
http://www.mbl.edu/
http://www.courses.mbl.edu/


From daemon Wed Mar 20 14:48:16 2002



From: robert.fowler-at-tdktca.com
Date: Wed, 20 Mar 2002 15:44:19 -0500
Subject: Old Filaments

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers I have recently found some old filaments for my JEOL T220-A.
The approximate age is 9+ years maybe less. Inspection at 100x shows some
unusually roughness on the filament. Is this normal? After an attempt to
use one the life was 2 hours. Should I discard or was this just a bad
filament? I have about 10 left and really hate to throw them away. Any help
will be appreciated

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



From daemon Wed Mar 20 14:52:26 2002



From: Jim Haley :      haley-at-mvia.com
Date: Wed, 20 Mar 2002 15:47:26 -0500
Subject: Re: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frederick,

Along the lines of the $1,500 price tag, you can pickup the Coolpix for
around $750.00 street price.

We offer adapters for all major microscope manufacturers (Leica,
Olympus, Zeiss, Nikon and Mitutoyo) that will clamp directly onto the
microscope and have the optics specifically designed for the Coolpix for
around $350.00. So the price tag for camera and adapter would be closer
to $1,100 (which will save you around $400).

If you want to make adapting the Coolpix to a microscope as easy as
possible, you can call us with a microscope model and manufacturer.
We'll get the correct adapter for the microscope with a single phone
call (which will save you a lot of headaches).

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
12901 Duckettown Road
Laurel, MD 20708
voice: (301) 809-6292
fax: (301) 805-2819
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

"Monson, Frederick C." wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Your Grace*,
}
} While the Coolpix 995 is an easy and ubiquitous choice, the
} $1,500.00 price (for camera, adapter, and remote from US Nikon microscope
} vendors) should be less now but isn't, though the pieces can be purchased
} separately at lower price and greater difficulty. You might want to look at
} the newer Coolpix 5000 which is currently available under the $1,000(USD)
} mark - not including remote or adapter but has been mentioned on the list of
} late.
}
} I cannot speak to the issue of quality of the CCD's as I am only
} just into the matter myself.
}
} Yours Sincerely,
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} West Chester University
} West Chester, Pennsylvania, USA, 19383
} 610-738-0437
} fmonson-at-wcupa.edu
}
} *(Ref:
} http://www.parliament.the-stationery-office.co.uk/pa/ld/ldinfo/ldadds.htm)
}
} } ----------
} } From: Vr. Richard Bejsak-Colloredo-Mansfeld
} } Reply To: Vr. Richard Bejsak-Colloredo-Mansfeld
} } Sent: Tuesday, March 19, 2002 10:23 PM
} } To: microscopy-at-sparc5.microscopy.com
} } Cc: coleoptera-at-yahoogroups.com
} } Subject: Digital camera
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I just have to buy camera for taking pictures of beetles directly or
} } throughs stereomicroscope Zeiss Jena Technoval.
} }
} } If I follow properly all discussions on this listserver the most used
} } camera
} } in Nikon Coolpix 995, isn't it?
} }
} } Thank you very much for your kind reply
} }
} } Keep care and be of good cheer
} }
} } Regards
} }
} } (name) Vratislav Richard Eugene Maria John Baptist
} } (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
} } (title) 84 duke of Siebenlügner
} }
} } websites:
} } http://www.coleoptera.org. and
} } http://www.egroups.com/group/coleoptera
} }
} } University of Sydney
} } The Wentworth Bldg., B 62
} } NSW 2006
} } AUSTRALIA
} } phone : +61 414 540 465
} } email: vratislav-at-bigfoot.com
} } ICQ: 13610107
} }
} } Only after the last tree has been cut down,
} } only after the last river has been poisoned,
} } only after the last fish has been caught,
} } only then will you find that money can not be eaten.'
} } CREE INDIAN PROPHECY.
} }
} } Incoming mail is certified Virus Free.
} } Checked by AVG anti-virus system (http://www.grisoft.com).
} }
} }
} }
} }

--



From daemon Wed Mar 20 15:26:21 2002



From: Sridhar Ramamurthy :      sramamur-at-uwo.ca
Date: Wed, 20 Mar 2002 16:19:21 -0500
Subject: ASM Surface Engineering Congress.

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues:

I take this opportunity to invite you to present a paper in ASM’s
Surface Engineering Congress to be held during ASM Fall Meeting, 7-10
October 2002, Columbus, Ohio. The Surface Engineering Congress will be
held in conjunction with 13th Annual International Federation for heat
Treatment. The announcement for the congress can be viewed at the
following web site:
http://www.asminternational.org/content/Conferences_Expos/CallForPapers/surface.htm

You are encouraged to submit a 100-150 words abstract. The abstract must
include complete name, title, affiliation, mailing address, telephone
and fax numbers as well as email address. The deadline for submitting
your abstract is 2 April 2002. You are required to submit the abstract
electronically. The instructions for electronic submission of abstract
are available at the end of the announcement available at above
mentioned web site.

A peer reviewed conference proceedings will be published and distributed
to all participants following the event. Deadline for manuscripts
submission will be September 2002. Authors of accepted papers will also
be invited to submit a written paper for journals such as the “Journal
of Thermal Spray Technology”, “Journal of Materials Engineering and
Performance”, and “Journal of Surface Engineering ”. PREPARATION OF
MANUSCRIPT IS ENCOURAGED BUT NOT OBLIGATORY.

Look forward to receiving your response soon. Please feel free to
contact me if you have any questions.

Regards,

Sridhar Ramamurthy
ASM Surface Engineering Congress
__________________________________________
Research Scientist Tel.: 1 519 661 2173
Surface Science Western Fax.: 1 519 661 3709
The University of Western Ontario Email: sramamur-at-uwo.ca
London, Ontario N6A 5B7, Canada Web: http://www.uwo.ca/ssw/


From daemon Wed Mar 20 16:05:50 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 20 Mar 2002 13:59:15 -0800
Subject: Re: TEM: Fixation of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was using permanganate fixation (instead osmium) and Spurr
embedding. There is recent very nice review on this subject, I find useful:

Robin Wright
Transmission Electron Microscopy of Yeasts.
Microscopy Research and Technique 51:496-510 (2000)
Good luck, Sergey

At 11:06 AM 3/20/02, you wrote:
} --------------------------------------------------------
} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Mar 20 16:19:22 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 20 Mar 2002 16:07:16 -0600
Subject: Re: Need freeze-sub: Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

Thanks to all who replied to my inquiry about high-pressure freezers and freeze-substitution units near central Missouri (and elsewhere!). I've passed on the information to our client.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Wed Mar 20 17:00:10 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 20 Mar 2002 14:53:42 -0800
Subject: Re: Old Filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Robert

I am using 8+ years old W filaments for my JEM1200EX TEM without problem. I
am not sure, how old they are: when I come in the Lab 8 years ago, they
were here (and were not new at that time, I guess). W could oxidize
slightly, may be you need to heat it slower that new ones.

Sergey

At 12:44 PM 3/20/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Mar 20 18:06:28 2002



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Wed, 20 Mar 2002 18:58:52 -0500
Subject: Re: Help with Film Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can't answer about the scanners themselves. But I have made a recent
discovery regarding the driver software. I have a Nikon LS-1000. I was about
to buy a new scanner, believing the density range of my scanner to be
inadequate - My highlights were flat and I couldn't hold detail in the
shadows, either. In the process of searching about for scanner information,
I stumbled upon SilverFast by LaserSoft. I downloaded the demo and was
sufficiently impressed that I bought the software the next day.

One would think that scanner manufacturers would provide software that would
take full advantage of their machine's capabilities. Such apparently is not
the case. Whichever scanner you buy, I suggest that you try out the
Silverfast demo after you have scanned a few images with the manufacturer's
software.

After you have made your choice it would be nice if you could report back on
what drove your decision. I may still buy a new scanner and would like to
hear about what's out there.

Bruce Girrell



From daemon Thu Mar 21 00:02:10 2002



From: charles j day :      wa5ekh-at-juno.com
Date: Mon, 18 Mar 2002 23:52:22 -0600
Subject: Electroscan (FEI) E3 Users?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking into potential improvements, continuous improvement
programs, upgrades, and current user opinions of potential and current
performance of these older variety ESEMs. In some ways these instruments
appear to be unique.
Agree? I would be interested in exploring any undeveloped potential of
these instruments. I'm also interested in service issues and potential
solutions. Finally, I would be interested in finding out specifically how
CeB6 filaments perform in these instruments. Also if you know of anyone
with one of these instruments please forward this to them or send me an
email. Thanks.
Jeff Day
Email: wa5ekh-at-juno.com
Note: I have completed some preliminary searches of applications for
these instruments.


From daemon Thu Mar 21 00:49:31 2002



From: =?iso-8859-2?Q?Krzysztof_Jan_H=FCbner?= :      hubner-at-IOd.krakow.pl
Date: Thu, 21 Mar 2002 07:23:36 +0100
Subject: Fw: ASM Surface Engineering Congress.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} Dear Colleagues:
}
} I take this opportunity to invite you to present a paper in ASM’s
} Surface Engineering Congress to be held during ASM Fall Meeting, 7-10
} October 2002, Columbus, Ohio. The Surface Engineering Congress will be
} held in conjunction with 13th Annual International Federation for heat
} Treatment. The announcement for the congress can be viewed at the
} following web site:
}
http://www.asminternational.org/content/Conferences_Expos/CallForPapers/surf
ace.htm
}
} You are encouraged to submit a 100-150 words abstract. The abstract must
} include complete name, title, affiliation, mailing address, telephone
} and fax numbers as well as email address. The deadline for submitting
} your abstract is 2 April 2002. You are required to submit the abstract
} electronically. The instructions for electronic submission of abstract
} are available at the end of the announcement available at above
} mentioned web site.
}
} A peer reviewed conference proceedings will be published and distributed
} to all participants following the event. Deadline for manuscripts
} submission will be September 2002. Authors of accepted papers will also
} be invited to submit a written paper for journals such as the “Journal
} of Thermal Spray Technology”, “Journal of Materials Engineering and
} Performance”, and “Journal of Surface Engineering ”. PREPARATION OF
} MANUSCRIPT IS ENCOURAGED BUT NOT OBLIGATORY.
}
} Look forward to receiving your response soon. Please feel free to
} contact me if you have any questions.
}
} Regards,
}
} Sridhar Ramamurthy
} ASM Surface Engineering Congress
} __________________________________________
} Research Scientist Tel.: 1 519 661 2173
} Surface Science Western Fax.: 1 519 661 3709
} The University of Western Ontario Email: sramamur-at-uwo.ca
} London, Ontario N6A 5B7, Canada Web: http://www.uwo.ca/ssw/



From daemon Thu Mar 21 02:53:08 2002



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 21 Mar 2002 02:49:10 -0800
Subject: RE: Old Filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No real aging problems that I am aware of. Surface roughness is fairly
normal, under microscopic examination. Any chance that your vacuum is not
up to snuff?

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, March 20, 2002 12:44 PM,
"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com
[SMTP:"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Listers I have recently found some old filaments for my JEOL
T220-A.
} The approximate age is 9+ years maybe less. Inspection at 100x shows some
} unusually roughness on the filament. Is this normal? After an attempt to
} use one the life was 2 hours. Should I discard or was this just a bad
} filament? I have about 10 left and really hate to throw them away. Any
help
} will be appreciated
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}
}
}
}



From daemon Thu Mar 21 07:25:46 2002



From: robert.fowler-at-tdktca.com
Date: Thu, 21 Mar 2002 08:19:25 -0500
Subject: Re: Old Filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Yes they are new JEOL "K" type filaments. I will take everyone's advice and
have the retipped. Thank you

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



"Garber,
Charles A." To: "robert.fowler-at-tdktca.com"
{cgarber-at-2spi. {robert.fowler-at-tdktca.com}
com} cc:
Subject: Re: Old Filaments
03/20/2002
10:01 PM






-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hi Robert,

Are these new JEOL "K" type filaments are these "retipped" and if retipped,
then is there any clue as to who did the retipping?

Chuck

SPI Supplies
-------- REPLY, Original message follows --------

} Date: Wednesday, 20-Mar-02 03:44 PM
}
} From: robert.fowler-at-tdktca.com-at-sparc5.microscopy. \ Internet:
} (robert.fowler-at-tdktca.com-at-sparc5.)
} To: MICROSCOPY BB \ Internet:
} (microscopy-at-sparc5.microscopy.com)
}
} Subject: Old Filaments
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To

-------- REPLY, End of original message --------







From daemon Thu Mar 21 08:26:59 2002



From: Dave Hall :      daveh-at-restechimage.com
Date: Thu, 21 Mar 2002 09:17:58 -0500
Subject: Re: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers:

In the interest of providing (hopefully) useful information, please be
aware of several things we have observed:

-We have seen the Nikon Coolpix 995 offered for as low as $670. Yes,
this is the full USA-market product. Yes, from reputable dealers
familiar with digital imaging and microscopy, not just the mail-order
photo web-sites. Yes the product is "as shipped, complete, from Nikon",
with full warranty and all accessories included.

-Also be aware that the couplers required to adapt the Nikon Coolpix 995
(as well as a multitude of other digital cameras) to microscopes (from
most manufacturers) can also be had from a number of knowledgeable
sources nationwide.

-And, chances are also good that there are a number of
imaging/microscopy dealers capable of providing the simplicity of a
single phone call solution, perhaps even for package prices around or
under $1,000. You may have to do a little homework, but they can be
found.

To answer the actual original question about whether or not the 995 is
the most used (or best) choice would require opinions from a good sample
of list posters. And, the answer, as always, comes down to much more
than street price.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - USA Phone(614) 921-0045




-----Original Message-----
} From: Jim Haley [mailto:haley-at-mvia.com]
Sent: Wednesday, March 20, 2002 3:47 PM
To: Monson, Frederick C.
Cc: 'List-Microscopy'


Frederick,

Along the lines of the $1,500 price tag, you can pickup the Coolpix for
around $750.00 street price.

We offer adapters for all major microscope manufacturers (Leica,
Olympus, Zeiss, Nikon and Mitutoyo) that will clamp directly onto the
microscope and have the optics specifically designed for the Coolpix for
around $350.00. So the price tag for camera and adapter would be closer
to $1,100 (which will save you around $400).

If you want to make adapting the Coolpix to a microscope as easy as
possible, you can call us with a microscope model and manufacturer.
We'll get the correct adapter for the microscope with a single phone
call (which will save you a lot of headaches).

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
12901 Duckettown Road
Laurel, MD 20708
voice: (301) 809-6292
fax: (301) 805-2819
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

"Monson, Frederick C." wrote:
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
} Your Grace*,
}
} While the Coolpix 995 is an easy and ubiquitous choice, the
} $1,500.00 price (for camera, adapter, and remote from US Nikon
microscope
} vendors) should be less now but isn't, though the pieces can be
purchased
} separately at lower price and greater difficulty. You might want to
look at
} the newer Coolpix 5000 which is currently available under the
$1,000(USD)
} mark - not including remote or adapter but has been mentioned on the
list of
} late.
}
} I cannot speak to the issue of quality of the CCD's as I am
only
} just into the matter myself.
}
} Yours Sincerely,
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} West Chester University
} West Chester, Pennsylvania, USA, 19383
} 610-738-0437
} fmonson-at-wcupa.edu
}
} *(Ref:
}
http://www.parliament.the-stationery-office.co.uk/pa/ld/ldinfo/ldadds.ht
m)
}
} } ----------
} } From: Vr. Richard Bejsak-Colloredo-Mansfeld
} } Reply To: Vr. Richard Bejsak-Colloredo-Mansfeld
} } Sent: Tuesday, March 19, 2002 10:23 PM
} } To: microscopy-at-sparc5.microscopy.com
} } Cc: coleoptera-at-yahoogroups.com
} } Subject: Digital camera
} }
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
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} }
-----------------------------------------------------------------------.
} }
} }
} } I just have to buy camera for taking pictures of beetles directly or
} } throughs stereomicroscope Zeiss Jena Technoval.
} }
} } If I follow properly all discussions on this listserver the most
used
} } camera
} } in Nikon Coolpix 995, isn't it?
} }
} } Thank you very much for your kind reply
} }
} } Keep care and be of good cheer
} }
} } Regards
} }
} } (name) Vratislav Richard Eugene Maria John Baptist
} } (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
} } (title) 84 duke of Siebenlügner
} }
} } websites:
} } http://www.coleoptera.org. and
} } http://www.egroups.com/group/coleoptera
} }
} } University of Sydney
} } The Wentworth Bldg., B 62
} } NSW 2006
} } AUSTRALIA
} } phone : +61 414 540 465
} } email: vratislav-at-bigfoot.com
} } ICQ: 13610107
} }
} } Only after the last tree has been cut down,
} } only after the last river has been poisoned,
} } only after the last fish has been caught,
} } only then will you find that money can not be eaten.'
} } CREE INDIAN PROPHECY.
} }
} } Incoming mail is certified Virus Free.
} } Checked by AVG anti-virus system (http://www.grisoft.com).
} }
} }
} }
} }

--




From daemon Thu Mar 21 08:35:13 2002



From: Humphrey, Charles :      cdh1-at-cdc.gov
Date: Thu, 21 Mar 2002 09:27:43 -0500
Subject: TEM-Negative stain standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listserver,

A friend is interested in a commercial source of standardized microspheres
(40-100nm glass beads etc.) that have been carefully defined for size and
for concentration. Concentration standardization should be based upon
particles per ml as well as for weight percentage in suspension. Does
anyone sell such a product? I am aware of Polysciences microspheres but
their description indicates weight percentage only.

Charles D. Humphrey
Centers for Disease Control and Prevention
Mailstop G30
1600 Clifton Rd.
Atlanta GA 30333
Tel: 404-639-3307



From daemon Thu Mar 21 08:49:04 2002



From: Mark YEADON :      m-yeadon-at-imre.org.sg
Date: Thu, 21 Mar 2002 22:45:20 +0800
Subject: HREM samples: HOPG using sticky tape?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I am trying to obtain HREM samples from a block of highly-ordered pyrolitic
graphite (HOPG), 1cm2 by 2mm (bought from a supplier). I've been using the
sticky-tape technique to peel a few layers off the block, and then strip
away layer after layer of this peeled layer with more sticky tape,which I
believe is a well-known technique.

I now need to remove the sellotape - this used to be done with acetone, but
the problem is, the modern recipe for Sellotape glue seems to be no longer
acetone-soluble (I even brought some sellotape to Singapore from UK to try).
I have tried hi-purity Tetrahydrofuran (THF) and it dissolves the glue layer
beautifully, but I seem to get a thick amorphous residue on the graphite
despite many rinses.

Is anyone still using this technique? Can anyone help me?

Many thanks!

Mark

%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119260

http://www.matsci.nus.edu.sg/STAFF/Mark.html
TEL: (+65) 874 8591
FAX: (+65) 872 0785
Email: m-yeadon-at-imre.org.sg


From daemon Thu Mar 21 08:54:29 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 21 Mar 2002 09:34:17 -0500
Subject: TEM: Heating carbon support films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have some samples on carbon support films. I would like to heat them in air. Does anyone know the upper limit in temperature that the carbon films will still survive?

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)




From daemon Thu Mar 21 10:03:33 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Thu, 21 Mar 2002 10:54:33 -0500
Subject: Old Filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robert,

There are many factors that could contribute to what happened in your case.
I will attempt to cover them in some detail. If you have additional
questions, I would be happy to speak with you.

The age of the filaments shouldn't matter. If stored under normal inside
conditions, not exposed to any chemicals and handled properly, I would be
very surprised if environmental factors had anything to do with their
appearance or premature failure.

There are different grades of tungsten wire commercially available. All
tungsten wire contains visible striations until current has been passed
through it. The better the tungsten, the fewer the striations. We use only
high grade tungsten wire. In manufacturing, we go through a process of
repeatedly vacuum annealing and re-centering every filament until it stays
centered. This stress relieves the tungsten and ensures filament stability
and long life, as well as smoothes the surface of the filaments, giving them
a shiny appearance. It is also very useful for quality control, in that
filaments with flaws in the tungsten wire will probably fail during the
process, instead of in our customers' instruments. The filaments you have
were probably not vacuum annealed and may also have been made of a low grade
tungsten. This may account for the rough appearance your seeing.

The early failure of the one filament you tried could have been caused by a
number of factors. Not having been vacuum annealed, that particular piece
of tungsten wire could have had a flaw in it that would not have been
detected by the manufacturer. Other factors could have been the condition
and/or operation of your instrument. Was the vacuum adequate and the
instrument properly set up or did you heat the filament too quickly? We can
generally tell by looking at a failed filament if it was a system problem.
For instance, if it is oxidized at the base of the tungsten wire, close to
the posts, that would typically mean a poor vacuum.

I would recommend that you check your instrument out and give another
filament a try. If you like, you could send us the failed one and we would
be happy to analyze it for you.

I hope this helps.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"


Disclaimer: Energy Beam Sciences is a manufacturer and distributor of
Tungsten, LaB6 and Field Emission electron beam sources.




-----Original Message-----
} From: "robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com
[mailto:"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com]
Sent: Wednesday, March 20, 2002 3:44 PM
To: Microscopy-at-sparc5.microscopy.com


Hello Listers I have recently found some old filaments for my JEOL T220-A.
The approximate age is 9+ years maybe less. Inspection at 100x shows some
unusually roughness on the filament. Is this normal? After an attempt to
use one the life was 2 hours. Should I discard or was this just a bad
filament? I have about 10 left and really hate to throw them away. Any help
will be appreciated

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com





From daemon Thu Mar 21 10:47:27 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Thu, 21 Mar 2002 11:41:25 -0500
Subject: Re: Electroscan (FEI) E3 Users?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Funny this comes up. I was just exploring the possibility of installing CeB6 in my XL-30 ESEM and would appreciate feedback from users who run it. Current/beam drift is always present with tungsten or LaB6 filaments installed in my machine. Is CeB6 truly more stable?? Anything I should be aware of or do we just treat it delicately like a LaB6 source?

Scott Whittaker
SEM Lab Manager
Smithsonian Institution
PO Box 37012 MRC104
National Museum of Natural History
Washington DC 20013-7012
202-357-1651


} } } charles j day {wa5ekh-at-juno.com} 03/19/02 12:52AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am looking into potential improvements, continuous improvement
programs, upgrades, and current user opinions of potential and current
performance of these older variety ESEMs. In some ways these instruments
appear to be unique.
Agree? I would be interested in exploring any undeveloped potential of
these instruments. I'm also interested in service issues and potential
solutions. Finally, I would be interested in finding out specifically how
CeB6 filaments perform in these instruments. Also if you know of anyone
with one of these instruments please forward this to them or send me an
email. Thanks.
Jeff Day
Email: wa5ekh-at-juno.com
Note: I have completed some preliminary searches of applications for
these instruments.




From daemon Thu Mar 21 10:52:03 2002



From: Norman_Michaud-at-meei.harvard.edu (by way of MicroscopyListserver)
Date: Thu, 21 Mar 2002 10:44:08 -0600
Subject: Re: Help with film scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently bought a multi pro and actually I find that it works fine
for EM negatives. The problem is that the area scanned and saved is
only 6x9 cm which is actually just a little less area than the 3x
enlargement I make in the darkroom. The multi format carrier holds
the negative without cutting and there's room in the carrier to move
the negative around to get features on the edge. The firewire
connection gives a 2400 dpi scan in 1-2 minutes. I've just begun
learning how to use it but I think it will be fine for my negatives.

No financial interest.

Norm Michaud
Mass Eye and Ear Infirmary
Boston, MA


From daemon Thu Mar 21 10:52:48 2002



From: Eric Steel :      eric.steel-at-nist.gov (by way of MicroscopyListserver)
Date: Thu, 21 Mar 2002 10:44:56 -0600
Subject: Quantitative Electron Probe Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


NIST-MAS Topical Workshop:
Understanding the Accuracy Barrier of Quantitative Electron Beam
X-ray Microanalysis and the Role of Standards

The workshop scheduled to be held at NIST, Gaithersburg, MD, April
8-11, 2002 is filled to capacity. If you are interested in viewing
it on a live web broadcast, contact Ryna Marinenko via email at
ryna.marinenko-at-nist.gov to get the URL of the web site. The web
address will not be available through any other means. When the
address is available, it will be emailed to the people that have
requested web access. (Note that you will need RealPlayer or
equivalent, available free from http://www.real.com/ * )

A listing of scheduled presentations and discussion topics can be
accessed at the following web site:
http://www.cstl.nist.gov/div837/Division/meetings/EPMAAccuracy/EProbeAccuracy.htm

Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-417-1321
100 Bureau Drive, Stop 8371
Gaithersburg, MD 20899-8371
http://www.nist.gov/micro

*Any mention of commercial products is for information only; it does
not imply recommendation or endorsement by NIST.


From daemon Thu Mar 21 11:31:23 2002



From: Chad Parish :      chad_parish-at-hotmail.com
Date: Thu, 21 Mar 2002 12:23:43 -0500
Subject: TEM -- problem with magnetic foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings to all from a new member of the listserver!

I'm trying to image thin foils of magnetic steel in a JEOL 200CX
200kV TEM, using both single- and double-tilt holders.

I can get good images, but I've been having serious trouble getting the
Z-height properly eucentric, which will throw off all my calibrations for
quantitative work, and also makes tilting to a ZA difficult.

The techniques I learned when getting my training (on non-magnetic foils,
naturally) to get the Z-height correct just don't seem to work. I do
everything possible to get the samples thinned ( {~3 milli-inch before
punching) to minimize the magnetic aberrations, but I'm still having serious
trouble.

Can anyone help me?

Thanks!

Chad Parish
Graduate Student,
Material Science and Engineering,
University of Pittsburgh


_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp.



From daemon Thu Mar 21 13:06:37 2002



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Thu, 21 Mar 2002 12:57:27 -0600
Subject: Near IR quenching: polyester membranes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anybody have information pertaining to the absorbtion spectra of
polyester tissue culture membranes? Specifically, I'm looking at cell
membranes labeled with FM 4-64 on cells cultured on Corning-Costar
Transwell Clear membranes. The emmission spectra of FM 4-64 appears to be
attenuated above 650nm in this instance, so I am wondering if the polyester
membranes are quenching fluorescence from FM 4-64. The emmission spectra of
FM 4-64 is supposed to peak at about 750nm, I'm getting peak emmission at
650nm and then a drop to baseline. Thanks in advance.
-Karl G.

_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Thu Mar 21 13:10:21 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 21 Mar 2002 19:09:12 +0000 (GMT Standard Time)
Subject: Re: TEM -- problem with magnetic foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chad,

First of all be very careful putting magnetic specimens in
the TEM. Unless they are properly clamped and the tilt
mechanism of the second tilt is a positive one (not
friction driven) then you may lose your specimen or
specimen and tilt gimbal in the column. You are right to
keep the amount of material to a minimum by thinning as
much as possible. If you do lose your specimen it will be
on the pole piece!

The best way to set your eucentric height is exactly how
you were shown, with a non magnetic sample. Having done so
remove the sample and insert your magnetic one. Now focus
the specimen using the eucetric height control. This will
set your specimen to the eucentric height. If you want a
more accurate method for calibration, measure the objective
lens current at the eucentric height (non magnetic
specimen) and keep it as close to that as possible during
calibration and your work.

You will find that many of the alignments change as you
tilt as you are moving a magnet (your specimen) around the
beam and constantly deflecting it. For the best imaging
avoid the edge of a hole, try to get (thin) material both
sides of the beam to even out the magnetic effect of the
specimen.

Good luck,
Ron


On Thu, 21 Mar 2002 12:23:43 -0500 Chad Parish
{chad_parish-at-hotmail.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings to all from a new member of the listserver!
}
} I'm trying to image thin foils of magnetic steel in a JEOL 200CX
} 200kV TEM, using both single- and double-tilt holders.
}
} I can get good images, but I've been having serious trouble getting the
} Z-height properly eucentric, which will throw off all my calibrations for
} quantitative work, and also makes tilting to a ZA difficult.
}
} The techniques I learned when getting my training (on non-magnetic foils,
} naturally) to get the Z-height correct just don't seem to work. I do
} everything possible to get the samples thinned ( {~3 milli-inch before
} punching) to minimize the magnetic aberrations, but I'm still having serious
} trouble.
}
} Can anyone help me?
}
} Thanks!
}
} Chad Parish
} Graduate Student,
} Material Science and Engineering,
} University of Pittsburgh
}
}
} _________________________________________________________________
} Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp.
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu Mar 21 13:51:47 2002



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Thu, 21 Mar 2002 11:39:35 -0800
Subject: digital micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listers. Does anyone know of a program that will convert Gatan
digital micrograph dm3 files to tif files without reducing pixel depth and
dimensions ? We are unable to open the digital micrographs in Photoshop.
Thanks!
JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856



From daemon Thu Mar 21 14:05:05 2002



From: Peter Heimann :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Thu, 21 Mar 2002 20:58:31 +0100
Subject: Re: TEM-Negative stain standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An ideal standard is TMV (tobacco mosaic virus).
practically undestroyable, just store it in the refridgerator for
years/decades and it's a good control for a "good" negative stain.
peter heimann
**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************


From daemon Thu Mar 21 14:10:09 2002



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Thu, 21 Mar 2002 14:04:16 -0600
Subject: HPF thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all for your advice on HPF of leaf tissues. I am digesting
the many good tips and figuring out what to do next.

Bob

--
Robert R. Wise, Ph.D.
Associate Professor of Plant Physiology
Department of Biology and Microbiology
University of Wisconsin Oshkosh

On sabbatical from Sept. 2001 to Aug. 2002 at UW Madison
Botany Department
B217 Birge Hall
430 Lincoln Drive
Madison, WI 53706
(608) 262-4288 (phone)
(608) 262-7509 (fax)
wise-at-uwosh.edu
http://www.wisc.edu/biotron/Sharkey/
http://www.uwosh.edu/departments/biology/wise/wise.html


From daemon Thu Mar 21 18:00:08 2002



From: Jiang Liu :      jiangliu24680-at-hotmail.com
Date: Thu, 21 Mar 2002 18:52:05 -0500
Subject: Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Members:

I'm posting this message for my friend.

Requires a BS/MS in Chemistry or similar degree. Person must have
either AFM/SEM/TEM experience and 2+ yrs experience in polymer
characterization. There is an opportunity to work with a top group
of professionals, and opportunity for advancement. Any bio-medical
exp. is a plus.

If interested, please contact Adrian Ganson at
aganson-at-basilone-oliver.com directly (www.basilone-oliver.com,
Tel:770 649-0553, or Fax:770 649-0565).

Jiang Liu, PhD.
Research & Technology Center
ATOFINA Petrochemicals, Inc.

_________________________________________________________________
MSN Photos is the easiest way to share and print your photos:
http://photos.msn.com/support/worldwide.aspx



From daemon Thu Mar 21 18:07:44 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Thu, 21 Mar 2002 16:02:00 -0800
Subject: Re: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;
If you look at a few digital camra web sites, you will notice that
the Nikon 995 and all the Coolpix family are obsolete cameras. Don't buy one
unless you WANT an obsolete camera, don't pay more for it than you would for
an obsolete camera-demand a price appropriate for an obsolete camera!

John Mardinly
Intel






Hello Listers:

In the interest of providing (hopefully) useful information, please be
aware of several things we have observed:

-We have seen the Nikon Coolpix 995 offered for as low as $670. Yes,
this is the full USA-market product. Yes, from reputable dealers
familiar with digital imaging and microscopy, not just the mail-order
photo web-sites. Yes the product is "as shipped, complete, from Nikon",
with full warranty and all accessories included.

-Also be aware that the couplers required to adapt the Nikon Coolpix 995
(as well as a multitude of other digital cameras) to microscopes (from
most manufacturers) can also be had from a number of knowledgeable
sources nationwide.

-And, chances are also good that there are a number of
imaging/microscopy dealers capable of providing the simplicity of a
single phone call solution, perhaps even for package prices around or
under $1,000. You may have to do a little homework, but they can be
found.

To answer the actual original question about whether or not the 995 is
the most used (or best) choice would require opinions from a good sample
of list posters. And, the answer, as always, comes down to much more
than street price.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - USA Phone(614) 921-0045




-----Original Message-----
} From: Jim Haley [mailto:haley-at-mvia.com]
Sent: Wednesday, March 20, 2002 3:47 PM
To: Monson, Frederick C.
Cc: 'List-Microscopy'


Frederick,

Along the lines of the $1,500 price tag, you can pickup the Coolpix for
around $750.00 street price.

We offer adapters for all major microscope manufacturers (Leica,
Olympus, Zeiss, Nikon and Mitutoyo) that will clamp directly onto the
microscope and have the optics specifically designed for the Coolpix for
around $350.00. So the price tag for camera and adapter would be closer
to $1,100 (which will save you around $400).

If you want to make adapting the Coolpix to a microscope as easy as
possible, you can call us with a microscope model and manufacturer.
We'll get the correct adapter for the microscope with a single phone
call (which will save you a lot of headaches).

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
12901 Duckettown Road
Laurel, MD 20708
voice: (301) 809-6292
fax: (301) 805-2819
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

"Monson, Frederick C." wrote:
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
} Your Grace*,
}
} While the Coolpix 995 is an easy and ubiquitous choice, the
} $1,500.00 price (for camera, adapter, and remote from US Nikon
microscope
} vendors) should be less now but isn't, though the pieces can be
purchased
} separately at lower price and greater difficulty. You might want to
look at
} the newer Coolpix 5000 which is currently available under the
$1,000(USD)
} mark - not including remote or adapter but has been mentioned on the
list of
} late.
}
} I cannot speak to the issue of quality of the CCD's as I am
only
} just into the matter myself.
}
} Yours Sincerely,
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} West Chester University
} West Chester, Pennsylvania, USA, 19383
} 610-738-0437
} fmonson-at-wcupa.edu
}
} *(Ref:
}
http://www.parliament.the-stationery-office.co.uk/pa/ld/ldinfo/ldadds.ht
m)
}
} } ----------
} } From: Vr. Richard Bejsak-Colloredo-Mansfeld
} } Reply To: Vr. Richard Bejsak-Colloredo-Mansfeld
} } Sent: Tuesday, March 19, 2002 10:23 PM
} } To: microscopy-at-sparc5.microscopy.com
} } Cc: coleoptera-at-yahoogroups.com
} } Subject: Digital camera
} }
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } I just have to buy camera for taking pictures of beetles directly or
} } throughs stereomicroscope Zeiss Jena Technoval.
} }
} } If I follow properly all discussions on this listserver the most
used
} } camera
} } in Nikon Coolpix 995, isn't it?
} }
} } Thank you very much for your kind reply
} }
} } Keep care and be of good cheer
} }
} } Regards
} }
} } (name) Vratislav Richard Eugene Maria John Baptist
} } (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
} } (title) 84 duke of Siebenlügner
} }
} } websites:
} } http://www.coleoptera.org. and
} } http://www.egroups.com/group/coleoptera
} }
} } University of Sydney
} } The Wentworth Bldg., B 62
} } NSW 2006
} } AUSTRALIA
} } phone : +61 414 540 465
} } email: vratislav-at-bigfoot.com
} } ICQ: 13610107
} }
} } Only after the last tree has been cut down,
} } only after the last river has been poisoned,
} } only after the last fish has been caught,
} } only then will you find that money can not be eaten.'
} } CREE INDIAN PROPHECY.
} }
} } Incoming mail is certified Virus Free.
} } Checked by AVG anti-virus system (http://www.grisoft.com).
} }
} }
} }
} }

--




From daemon Thu Mar 21 18:19:28 2002



From: evgenia.pekarskaya-at-exxonmobil.com
Date: Thu, 21 Mar 2002 19:13:54 -0500
Subject: Re: TEM -- problem with magnetic foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Chad,

Unfortunately this is a problem with magnetic specimens.
Minimizing specimen thickness helps. Try to have specimens not thicker than
50 microns. Try to have smaller specimens as well: half of the disk (rather
than the full disk) or even smaller. If you glue it onto a slot grid, make
sure that the specimen is properly fixed to the grid! Otherwise it will be
pulled out from the grid once it is in the microscope and this can cause a
problem for the microscope astigmatism!
Also, I read once that it is better to perform the Z-height correction on
magnetic specimens by tilting the specimen only on one side of the zero
tilt.

Good luck,
Evgenia


**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355




"Chad Parish"
{chad_parish-at-ho To: Microscopy-at-sparc5.microscopy.com
tmail.com} cc:
Subject: TEM -- problem with magnetic foils

03/21/02 12:23
PM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Greetings to all from a new member of the listserver!

I'm trying to image thin foils of magnetic steel in a JEOL 200CX
200kV TEM, using both single- and double-tilt holders.

I can get good images, but I've been having serious trouble getting the
Z-height properly eucentric, which will throw off all my calibrations for
quantitative work, and also makes tilting to a ZA difficult.

The techniques I learned when getting my training (on non-magnetic foils,
naturally) to get the Z-height correct just don't seem to work. I do
everything possible to get the samples thinned ( {~3 milli-inch before
punching) to minimize the magnetic aberrations, but I'm still having
serious
trouble.

Can anyone help me?

Thanks!

Chad Parish
Graduate Student,
Material Science and Engineering,
University of Pittsburgh


_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp.









From daemon Thu Mar 21 20:59:01 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 21 Mar 2002 18:56:39 -0800
Subject: RE: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That's all that every manufacturer does--they make
obsolete products. Intel is right there in the pack.
Their products are obsolete virtually as soon as they
are introduced. Think not?? Try to get support on a
one year old product. Some can be supported while
others cannot.

If an obsolete product will do the job, then so what?
The cost is lower and the performance is most likely
sufficient for the task. If today is the motive, go
for it.

Chasing the technological rainbow is stupid....if not
costly.

The Nikon 990 is a beautiful example of competent
technology. It is not obsolete--it is just not made anymore.
Big difference.

gary g.



At 04:02 PM 3/21/2002, you wrote:

} Folks;
} If you look at a few digital camra web sites, you will notice that
} the Nikon 995 and all the Coolpix family are obsolete cameras. Don't buy one
} unless you WANT an obsolete camera, don't pay more for it than you would for
} an obsolete camera-demand a price appropriate for an obsolete camera!
}
} John Mardinly
} Intel
}
}
}
}
}
}
} Hello Listers:
}
} In the interest of providing (hopefully) useful information, please be
} aware of several things we have observed:
}
} -We have seen the Nikon Coolpix 995 offered for as low as $670. Yes,
} this is the full USA-market product. Yes, from reputable dealers
} familiar with digital imaging and microscopy, not just the mail-order
} photo web-sites. Yes the product is "as shipped, complete, from Nikon",
} with full warranty and all accessories included.
}
} -Also be aware that the couplers required to adapt the Nikon Coolpix 995
} (as well as a multitude of other digital cameras) to microscopes (from
} most manufacturers) can also be had from a number of knowledgeable
} sources nationwide.
}
} -And, chances are also good that there are a number of
} imaging/microscopy dealers capable of providing the simplicity of a
} single phone call solution, perhaps even for package prices around or
} under $1,000. You may have to do a little homework, but they can be
} found.
}
} To answer the actual original question about whether or not the 995 is
} the most used (or best) choice would require opinions from a good sample
} of list posters. And, the answer, as always, comes down to much more
} than street price.
}
} *Kind Regards,
} *Dave Hall
} *Resolution Technology, Inc - USA Phone(614) 921-0045
}
}
}
}
} -----Original Message-----
} } From: Jim Haley [mailto:haley-at-mvia.com]
} Sent: Wednesday, March 20, 2002 3:47 PM
} To: Monson, Frederick C.
} Cc: 'List-Microscopy'
} Subject: Re: Digital camera
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Mar 21 21:23:16 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Thu, 21 Mar 2002 22:29:07 -0500
Subject: Re: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Perhaps I'm missing something but why should an adapter for the Coolpix
995 (or any comparable camera) cost half as much as the entire camera
costs at street prices? If something with the complexity of a megapixel
CCD camera, with a zoom lens, motor drives, focusing, color correction
and metering circuitry, storage card, etc. costs $700 why does a
threaded ring with three clamps cost upwards of $160 and one with an
internal lens cost $350?

I haven't yet tried the Coolpix for shots at higher than 500x, but up to
that mag it seems to work great right through the eyepiece.

John Twilley



From daemon Thu Mar 21 23:49:09 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 21 Mar 2002 21:40:08 -0500
Subject: Re: Hg Bulbs vs Finger Prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 3/20/02 12:02 PM, Monson, Frederick C. at fmonson-at-wcupa.edu wrote:

}
} Richard,
}
} I have never installed a Hg bulb without first 'cleaning' it with 50%
} ethanol in 2x processed water. I use halves of the lint-free cloth I have
} for the EM's to clean and then dry. When I change a bulb, I blow out the
} housing with my trusty little vacuum, before installing the new one. Not
} training or advice. Just an independent, personal approach.
}
} Then again, I used to keep ethyl ether in my refrigerator, dip my fingers in
} xylene to retrieve slides, dissect with no gloves, and clean up after I used
} someone else's space. What do I know.
}
} Fred Monson
}
} P.S.1 A Dean once demanded that I remove a sign on my office door that
} stated, "If I am reading, I AM working!"
}
} P.S.2. Someone really ran into my car this morning, and I am still feeling
} somewhat silly, though not a lot different from yesterday!
}
}
} } From: Richard Edelmann
} } Is it o.k., to remove (student) finger prints from a Hg bulb (for a
} } Epi-
} } illuminator lamp) with a solvent (EtOH or Acetone) and a lint free cloth?
} }
} } The bulb has NOT yet been installed or subjected to current/heat
} } (i.e. the
} } finger prints haven't been burned on to the glass).
} }
} } I'd rather not simply dispose of the new bulb (though diposing of
} } the
} } student who can't be bothered to read is a possibility).
} }
Dear Richard,
Fred has it right; EtOH is an excellent solvent for fingerprint grease,
and is not particularly toxic. I actually use 95% EtOH, but 50% should do
quite well.

Dear Fred,
If you put an agent into the EtOEt to prevent peroxide formation, you
can store it in your explosion-proof refrigerator; otherwise, you may become
eligible for a Darwin Award. Xylene can extract lipids from your skin and
cause it to become dry and cracked, but I have also dipped my fingers in
xylene without too much harm, especially if I wash and use a moisturizer
afterward.
Yours,
Bill Tivol



From daemon Thu Mar 21 23:58:08 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 21 Mar 2002 21:57:41 -0800
Subject: Re: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How about sheer numbers?

Say, two million cameras sold. Three hundred
microscope adapters sold. There is little margin
for profit in the niche areas. Thus, the selling
price is commensurate with that reality.

gary g.


At 07:29 PM 3/21/2002, you wrote:

} Perhaps I'm missing something but why should an adapter for the Coolpix
} 995 (or any comparable camera) cost half as much as the entire camera
} costs at street prices? If something with the complexity of a megapixel
} CCD camera, with a zoom lens, motor drives, focusing, color correction and
} metering circuitry, storage card, etc. costs $700 why does a threaded ring
} with three clamps cost upwards of $160 and one with an internal lens cost
} $350?
} I haven't yet tried the Coolpix for shots at higher than 500x, but up to
} that mag it seems to work great right through the eyepiece.
}
} John Twilley
}



From daemon Fri Mar 22 02:55:45 2002



From: Hyman, S.C. :      sch10-at-leicester.ac.uk
Date: Fri, 22 Mar 2002 08:47:35 -0000
Subject: Reichert Ultracut E manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

Is there any chance that someone 'out there' may have a Reichert
Ultracut E manual that may be spared/electronically shared?

TIA


Stefan


S.C. Hyman
Chief Technician
The Electron Microscope Laboratory
Faculty of Medicine and Biological Sciences
Adrian Building
University of Leicester
University Road
Leicester
LE1 7RH

Tel. (0116) 252 3370



From daemon Fri Mar 22 03:22:14 2002



From: Dr Adam Papworth :      ajp5-at-liverpool.ac.uk
Date: Fri, 22 Mar 2002 09:18:23 +0000
Subject: HOPG

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Mark,

The best way I know of making a TEM sample of HOPG is just to take a
lump of HOPG drop it into ultra high pure ethanol and then use
ultrasonic and vibrate it for ten minutes. now using a pipette drop a
droplet on to a lacey carbon grid.

And there is your sample

--
Dr Adam Papworth,
Senior Experimental Officer,
Department of Engineering,
The University of Liverpool,
Liverpool,
L69 3GH, UK.

Phone
(Work) 0151 794 4672
(Mobile) 0151 794 7587
07970 24 7587
(Home) 0151 283 8596
(FAX) 0151 794 4675
e-mail ajp5-at-liv.ac.uk




From daemon Fri Mar 22 03:54:18 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Fri, 22 Mar 2002 10:51:37 +0100
Subject: Digital camera's

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Hi listers,

If you are looking for a digital camera that is cheap, but does not have to
be the very best, you might want to try the following:

Get a webcam, take off the lens and put the ccd-chip that's inside, in the
ocular-tube of the microscope. Connect the webcam to the pc, et voilà, you
can view your images online! Some webcams already have a resolution of 1,5
million pixels and cost less than $250!

Of course you'll have to find the right depth of the ccd-chip, but after
some tryouts,it works pretty well! I found this trick in the dutch
magazine Natuur & Techniek.

Have fun!

Sincerely,

Sven

___________________________________________
Sven Terclavers
Research Assistent
Center for Molecular and Vascular Biology
Center for Transgene Technology and Gene Therapy
Campus Gasthuisberg O/N Level 9
Herestraat 49 - 3000 Leuven - Belgium
Tel.: +32 (0)16 34 59 90
Fax: +32 (0)16 34 59 91
E-mail: Sven.Terclavers-at-med.kuleuven.ac.be
Web: www.kuleuven.ac.be/mcm
____________________________________________



From daemon Fri Mar 22 07:09:16 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 22 Mar 2002 08:05:19 -0500
Subject: Gallium Milling of 3-5 Compounds

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Hello Folks;

This message is to the semiconductor people that use FIB [Focused Ion Beam]
milling systems.

We use a Gallium source FIB system to electrically isolate elements in GaAs
R.F. devices in integrated circuits. There are some caveats to doing this
in that residual Ga from the beam remains behind as well as crystalline
damage to the underlying semi-insulating substrate. That means electrical
isolation between elements is not always consistent or repeatable. I plan to
attempt to improve this process by removing the residual Ga in the milled
area chemically. However, GaAs [the substrate material] etches a great deal
in solvents, acids and even D.I. water, albeit at a very slow rate in water.

Has anyone solved this problem with a specific technique that they would
like to share, or simply compare notes?

Regards,

Peter Tomic
Anadigics, Inc.
Warren, New Jersey
Failure Analysis & Analytical Services Group

Ps. Since many like to hang a good quote on their messages, let me add one
of my favorites. This is a paraphrase.

"The great thing about physics is that it works the same everywhere in the
universe, except for certain neighborhoods in New Jersey" Woody Allen


From daemon Fri Mar 22 07:10:04 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 22 Mar 2002 08:08:50 -0500
Subject: FW: Gallium Milling of 3-5 Compounds

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I hope everyone doesn't get this twice.

} -----Original Message-----
} From: Peter Tomic
} Sent: Friday, March 22, 2002 8:05 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: Gallium Milling of 3-5 Compounds
}
} Hello Folks;
}
} This message is to the semiconductor people that use FIB [Focused Ion
} Beam] milling systems.
}
} We use a Gallium source FIB system to electrically isolate elements in
} GaAs R.F. devices in integrated circuits. There are some caveats to doing
} this in that residual Ga from the beam remains behind as well as
} crystalline damage to the underlying semi-insulating substrate. That means
} electrical isolation between elements is not always consistent or
} repeatable. I plan to attempt to improve this process by removing the
} residual Ga in the milled area chemically. However, GaAs [the substrate
} material] etches a great deal in solvents, acids and even D.I. water,
} albeit at a very slow rate in water.
}
} Has anyone solved this problem with a specific technique that they would
} like to share, or simply compare notes?
}
} Regards,
}
} Peter Tomic
} Anadigics, Inc.
} Warren, New Jersey
} Failure Analysis & Analytical Services Group
}
} Ps. Since many like to hang a good quote on their messages, let me add one
} of my favorites. This is a paraphrase.
}
} "The great thing about physics is that it works the same everywhere in the
} universe, except for certain neighborhoods in New Jersey" Woody Allen


From daemon Fri Mar 22 08:21:23 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Fri, 22 Mar 2002 10:41:48 -0330
Subject: RE: digital micrograph

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JoAnn writes ...

} ... Does anyone know of a program that will convert Gatan
} digital micrograph dm3 files to tif files without reducing
} pixel depth and dimensions ? We are unable to open the
} digital micrographs in Photoshop. ...

You don't mention your computer platform, but for Windows ... "Irfanview"
was previously mentioned:
www.irfanview.com
.. and I've also been made aware of "XnView":
www.xnview.com

Unfortunately, I see no mention of your file format ... but these programs
will (including Photoshop) make an effort to open any format ... but the
format will need to be uncompressed, and you must know the bitmap dimensions
and depth (e.g., 1024x768 & 3 8bit channels).

With Photoshop: (1) File=} 'open as' = "raw" file type (2) the dialog
will ask you for the above information ... (3) including the header size,
which you probably don't know ... enter 'zero'. (4) Photoshop will now
prompt you "image is too small for file size" (implying compression, and you
need find another route), or "image too large ... open anyway?" (5) say yes
.. and you'll probably see the image divided into 2 parts ... which is the
problem with guessing wrong at the "header" size. (6) Try again ... and
enter the info as before, but now select the header size and then the
"guess" button, and PS will guess at the header size. (7) If it is again
wrong, it's because there exists also a file "footer", and PS's guess was
too high. (8) It's now up to you to guess the right "header" size, but
it's probably just a bit smaller than PS's guess ... and the good news is
.. the same header can (probably) be used for other dw3 files(?)

hth ... & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From daemon Fri Mar 22 09:05:01 2002



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Fri, 22 Mar 2002 08:57:41 -0600
Subject: RE: digital micrograph

Contents Retrieved from Microscopy Listserver Archives
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Jo Ann et al.

I have done this with a c program on Unix (I'll give the code free to anyone
who'd like to see some really cobbled programming!).

If you don't fiddle with the file (i.e. calculate a histogram etc.) the
header size is quite reproducibly 3842 bytes long. If you fiddle with the
image at all in DM, the header gets bigger as presumably some additional
info gets stored there.

The image data is stored in consecutive location as 16 bit unsigned integers
(at least in my case which was after collection with a Gatan CCD camera).

Now, it would be very civil if Gatan would provide a reader (executable)
which will simply translate our data to 16 bit TIF files ...

Wharton


} -----Original Message-----
} From: michael shaffer [SMTP:rarewolf-at-roadrunner.nf.net]
} Sent: Friday, March 22, 2002 8:12 AM
} To: JoAnn Buchanan; microscopy-at-sparc5.microscopy.com
} Subject: RE: digital micrograph
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} JoAnn writes ...
}
} } ... Does anyone know of a program that will convert Gatan
} } digital micrograph dm3 files to tif files without reducing
} } pixel depth and dimensions ? We are unable to open the
} } digital micrographs in Photoshop. ...
}
} You don't mention your computer platform, but for Windows ...
} "Irfanview"
} was previously mentioned:
} www.irfanview.com
} .. and I've also been made aware of "XnView":
} www.xnview.com
}
} Unfortunately, I see no mention of your file format ... but these
} programs
} will (including Photoshop) make an effort to open any format ... but the
} format will need to be uncompressed, and you must know the bitmap
} dimensions
} and depth (e.g., 1024x768 & 3 8bit channels).
}
} With Photoshop: (1) File=} 'open as' = "raw" file type (2) the dialog
} will ask you for the above information ... (3) including the header size,
} which you probably don't know ... enter 'zero'. (4) Photoshop will now
} prompt you "image is too small for file size" (implying compression, and
} you
} need find another route), or "image too large ... open anyway?" (5) say
} yes
} .. and you'll probably see the image divided into 2 parts ... which is the
} problem with guessing wrong at the "header" size. (6) Try again ... and
} enter the info as before, but now select the header size and then the
} "guess" button, and PS will guess at the header size. (7) If it is again
} wrong, it's because there exists also a file "footer", and PS's guess was
} too high. (8) It's now up to you to guess the right "header" size, but
} it's probably just a bit smaller than PS's guess ... and the good news is
} .. the same header can (probably) be used for other dw3 files(?)
}
} hth ... & cheerios ... shAf :o)
} Avalon Peninsula, Newfoundland
} www.micro-investigations.com (in progress)
}


From daemon Fri Mar 22 09:19:18 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Fri, 22 Mar 2002 07:14:16 -0800
Subject: Re: digital micrograph

Contents Retrieved from Microscopy Listserver Archives
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If you are using a Mac, then GraphiConverter will open and convert just
about anything. It's shareware and you can get it from:

{http://lemkesoft.com/us_index.html}

Lesley Weston.



on 21/03/2002 11:39 AM, JoAnn Buchanan at redhair-at-leland.stanford.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello listers. Does anyone know of a program that will convert Gatan
} digital micrograph dm3 files to tif files without reducing pixel depth and
} dimensions ? We are unable to open the digital micrographs in Photoshop.
} Thanks!
} JoAnn Buchanan
} Molecular and Cellular Physiology
} Stanford University School of Medicine
} Stanford, CA 94305
} 650-723-5856
}
}



From daemon Fri Mar 22 09:42:00 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 22 Mar 2002 15:34:33 +0000
Subject: Coating for FEGSEM

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Dear All

having acquired a FEGSEM, I am now only too well aware of the
shortcomings of traditional sputter coatings - thick, granular, detail-
smothering gloop obscuring ultrastructure. What do you currently
advise as the best and most cost-effective method of obtaining
quality coatings in the 1 to 2 nm range?

Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Fri Mar 22 10:05:11 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 22 Mar 2002 10:44:06 -0500
Subject: HREM samples: HOPG using sticky tape?

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I have done crystalline MoS2 with tape, but in a reverse way than you describe. I used low temperature wax such as Quick-Stick from South Bay Technology to attach my sample to a glass slide. Then I used tape to peel layers away until I got a sample that was very optically transparent. Then I dissolved the LT-wax in acetone, washed it in acetone, and captured the samples onto a grid. I got a several very good samples.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Mark YEADON [mailto:m-yeadon-at-imre.org.sg]
Sent: Thursday, March 21, 2002 9:45 AM
To: 'Microscopy-at-sparc5.microscopy.com'


Dear Colleagues,

I am trying to obtain HREM samples from a block of highly-ordered pyrolitic
graphite (HOPG), 1cm2 by 2mm (bought from a supplier). I've been using the
sticky-tape technique to peel a few layers off the block, and then strip
away layer after layer of this peeled layer with more sticky tape,which I
believe is a well-known technique.

I now need to remove the sellotape - this used to be done with acetone, but
the problem is, the modern recipe for Sellotape glue seems to be no longer
acetone-soluble (I even brought some sellotape to Singapore from UK to try).
I have tried hi-purity Tetrahydrofuran (THF) and it dissolves the glue layer
beautifully, but I seem to get a thick amorphous residue on the graphite
despite many rinses.

Is anyone still using this technique? Can anyone help me?

Many thanks!

Mark

%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119260

http://www.matsci.nus.edu.sg/STAFF/Mark.html
TEL: (+65) 874 8591
FAX: (+65) 872 0785
Email: m-yeadon-at-imre.org.sg


From daemon Fri Mar 22 11:02:01 2002



From: Bob Harris :      bharris-at-micro.uoguelph.ca
Date: Fri, 22 Mar 2002 11:53:05 -0500
Subject: EM 300 help and JEOL EMs

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Hello:

Just a note to say thank you to all who responded to my 2
postings. We have decided to do an hourly service agreement with
FEI on the 300 until we can afford the Hg pump change over. The
disposition of the 2 JEOLs will be decided by their owners who I
hope are in contact with interested parties. As we are moving in the
future I will probably post more equipment as available. Thanks
again. bob
Bob Harris
NSERC Regional STEM Facility
Dep't of Microbiology
University of Guelph
Guelph Ontario
Canada N1G 2W1
Phone: 519-824-4120 ext 6409
Fax: 519-837-1802


From daemon Fri Mar 22 11:58:14 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 22 Mar 2002 11:44:10 -0600
Subject: Re: digital micrograph

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JoAnn,

Try Graphic Converter, ver. 4.09 or later. I had this problem, and
sent Thorsten Lemke a DM file, for which he wrote a translator. Mind,
mine was version 2.5 something, so if this doesn't work, you could
try sending an image to Lemke for another upgrade.
The DM image is a modified TIF, I think, so this isn't hard to do.
http://www.lemkesoft.com

Phil

} Hello listers. Does anyone know of a program that will convert
} Gatan digital micrograph dm3 files to tif files without reducing
} pixel depth and dimensions ? We are unable to open the digital
} micrographs in Photoshop. Thanks!
} JoAnn Buchanan
} Molecular and Cellular Physiology
} Stanford University School of Medicine
} Stanford, CA 94305
} 650-723-5856

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Mar 22 12:18:02 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 22 Mar 2002 13:07:29 -0500
Subject: HOPG sample preparation

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Adam Papworth wrote:
================================================
The best way I know of making a TEM sample of HOPG is just to take a lump of
HOPG drop it into ultra high pure ethanol and then use ultrasonic and
vibrate it for ten minutes. now using a pipette drop a droplet on to a lacey
carbon grid.

And there is your sample
================================================
I could be wrong about this, but I would expect that whatever colloidal
sized solids that were detected this way would have been particulates
created during the cutting of the pieces in to the finally purchased
"blocks". There are different "grades" of HOPG and I think it would be
difficult to relate colloidal particles picked up this way to respective
grades.

I don't know of anyone who has ever been able to cleave HOPG into a thin
enough "strip" so that there was electron transparency. However, if anyone
has done that, I would be interested in hearing how you have done that.

Disclaimer: SPI Supplies is a major of supplier for AFM and thin film
coatings research.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Fri Mar 22 12:32:03 2002



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Fri, 22 Mar 2002 13:06:41 -0500
Subject: SEM/cryostage in Northeast USA?

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Please post or send recommendations regarding my student's request.
Thank you.
Jim


} Date: Fri, 22 Mar 2002 03:37:01 -0500
} From: Gregory Shenk {ashenk-at-snet.net}
} Reply-To: ashenk-at-snet.net
} Subject: freezing stage
}
}
} I was wondering if you know of any facilities within driving distance
} (from } Connecticut) that have a scanning electron microscope with a
} freezing stage? I } really think that's what I need to look at my lupine
} stigmas.
}
}
} Greg Shenk

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
Storrs, CT 06269-2242
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Fri Mar 22 13:43:23 2002



From: Anna Logvinova :      alogvinova-at-buckinstitute.org
Date: Fri, 22 Mar 2002 11:36:52 -0800
Subject: TEM grid trimming

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I am interested to purchase a pyramid maker and/or other device for semi-automatic trimming of my Epon flat-embedded cells in the thermanox sandwiches . Does anybody have a "favorite" for such trimming? Where should I look for it?

Thanks a lot in advance!

Anna Logvinova, M.D.
Morphology Core Supervisor
Buck Institute
8001 Redwood Blvd
Novato, CA 94948
www.buckinstitute.org

(415)209-2259
alogvinova-at-buckinstitute.org


From daemon Fri Mar 22 14:43:46 2002



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Fri, 22 Mar 2002 12:31:55 -0800
Subject: RE: digital micrograph

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Hello Jo Ann, and Everyone,
At the last M&M conference, 2001 in LA, there was a users meeting
that Gatan hosted. During the course of the meeting they stated that they
were discontinuing their development for DM for the Mac, and were going to
only support PC's. As a consolation, they offered to make their last
version of DM (3.4.4) available for free to those who asked for it.
So, one option for those using or having access to a Mac, would be
to obtain the free copy of DM, and then convert the images to "data only"
format. This saves the file as a 16bit raw data file that can be easily
opened by photoshop and probably other image programs. The files can also
be saved as 8 bit tiffs, but to get at the full 16 bit data, the trick is to
export in data only format.
Not a universal solution, but possibly useful to some.

-Brad

----------
From: Sinkler, Wharton
Sent: Friday, March 22, 2002 06:57
To: 'michael shaffer'; JoAnn Buchanan;
microscopy-at-sparc5.microscopy.com
Subject: RE: digital micrograph


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
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-----------------------------------------------------------------------.



Jo Ann et al.

I have done this with a c program on Unix (I'll give the code free
to anyone
who'd like to see some really cobbled programming!).

If you don't fiddle with the file (i.e. calculate a histogram etc.)
the
header size is quite reproducibly 3842 bytes long. If you fiddle
with the
image at all in DM, the header gets bigger as presumably some
additional
info gets stored there.

The image data is stored in consecutive location as 16 bit unsigned
integers
(at least in my case which was after collection with a Gatan CCD
camera).

Now, it would be very civil if Gatan would provide a reader
(executable)
which will simply translate our data to 16 bit TIF files ...

Wharton


} -----Original Message-----
} From: michael shaffer [SMTP:rarewolf-at-roadrunner.nf.net]
} Sent: Friday, March 22, 2002 8:12 AM
} To: JoAnn Buchanan; microscopy-at-sparc5.microscopy.com
} Subject: RE: digital micrograph
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} JoAnn writes ...
}
} } ... Does anyone know of a program that will convert Gatan
} } digital micrograph dm3 files to tif files without reducing
} } pixel depth and dimensions ? We are unable to open the
} } digital micrographs in Photoshop. ...
}
} You don't mention your computer platform, but for Windows ...
} "Irfanview"
} was previously mentioned:
} www.irfanview.com
} .. and I've also been made aware of "XnView":
} www.xnview.com
}
} Unfortunately, I see no mention of your file format ... but
these
} programs
} will (including Photoshop) make an effort to open any format ...
but the
} format will need to be uncompressed, and you must know the bitmap
} dimensions
} and depth (e.g., 1024x768 & 3 8bit channels).
}
} With Photoshop: (1) File=} 'open as' = "raw" file type (2) the
dialog
} will ask you for the above information ... (3) including the
header size,
} which you probably don't know ... enter 'zero'. (4) Photoshop
will now
} prompt you "image is too small for file size" (implying
compression, and
} you
} need find another route), or "image too large ... open anyway?"
(5) say
} yes
} .. and you'll probably see the image divided into 2 parts ...
which is the
} problem with guessing wrong at the "header" size. (6) Try again
.. and
} enter the info as before, but now select the header size and then
the
} "guess" button, and PS will guess at the header size. (7) If it
is again
} wrong, it's because there exists also a file "footer", and PS's
guess was
} too high. (8) It's now up to you to guess the right "header"
size, but
} it's probably just a bit smaller than PS's guess ... and the good
news is
} .. the same header can (probably) be used for other dw3 files(?)
}
} hth ... & cheerios ... shAf :o)
} Avalon Peninsula, Newfoundland
} www.micro-investigations.com (in progress)
}




From daemon Fri Mar 22 15:08:18 2002



From: Chad Friece :      cfriece-at-biocrystal.com
Date: Fri, 22 Mar 2002 16:01:42 -0500
Subject: Sample Preparation for TEM

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I have a question regarding sample preparation for Transmission electron microscopy. I am growing cells on a polystyrene surface which is in an enclosed environment. In this environment, the cells are fixed with gluteraldehyde. Following fixation, the polystyrene is sectioned or physically cut with a scalpel or a pair of scissors (with the fixed cells attached) and placed in buffer. The sections are dehydrated in increasing concentrations of EtOH. Next, the sections are put through a transition step in which they are exposed to 2-hydroxypropylmethacrylate, followed by embedding in an Epon derivative. My problem is somewhere between the transition step and the embedding step in which the polystyrene is actually being dissolved. Is there a method or reagent that can be used to prepare cells grown on polystyrene for TEM? I believe it would have to be some sort of water-based reagent/method because almost every organic solvent I have used has dissolved polystyrene. It has been suggested that I change the substrate for which the cells are grown; however, it is crucial that I use this polystyrene growth surface.

Any suggestions would be greatly appreciated.

Thank You,

Chad Friece
cfirece-at-biocrystal.com



From daemon Fri Mar 22 15:53:53 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Fri, 22 Mar 2002 13:41:16 -0800
Subject: Re: Gallium Milling of 3-5 Compounds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Peter:

Another option you may want to consider is low energy (100-200eV) argon ion
milling. This is used for, among other things, post FIB processing of TEM
samples and has been used to remove implanted Ga and also to remove amorphous
damage from the specimen. If you would like to get additional information on
this technology, please visit our website at
www.southbaytech.com and enter the keyword "TL-GM1". This will bring you
directly to the Gentle Mill.

I also have some images I could send you that shows the removal of amorphous
damage on GaAs after processing under low energy milling conditions.

Please let me know if you would like any addiitonal information.

DISCLAIMER: South Bay Technology produces equipment and supplies as described
above and, therefore, has a vested interest in promoting their use.

Best regards-

David

Peter Tomic wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Folks;
}
} This message is to the semiconductor people that use FIB [Focused Ion Beam]
} milling systems.
}
} We use a Gallium source FIB system to electrically isolate elements in GaAs
} R.F. devices in integrated circuits. There are some caveats to doing this
} in that residual Ga from the beam remains behind as well as crystalline
} damage to the underlying semi-insulating substrate. That means electrical
} isolation between elements is not always consistent or repeatable. I plan to
} attempt to improve this process by removing the residual Ga in the milled
} area chemically. However, GaAs [the substrate material] etches a great deal
} in solvents, acids and even D.I. water, albeit at a very slow rate in water.
}
} Has anyone solved this problem with a specific technique that they would
} like to share, or simply compare notes?
}
} Regards,
}
} Peter Tomic
} Anadigics, Inc.
} Warren, New Jersey
} Failure Analysis & Analytical Services Group
}
} Ps. Since many like to hang a good quote on their messages, let me add one
} of my favorites. This is a paraphrase.
}
} "The great thing about physics is that it works the same everywhere in the
} universe, except for certain neighborhoods in New Jersey" Woody Allen

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Fri Mar 22 16:20:52 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 22 Mar 2002 14:14:54 -0800 (PST)
Subject: Re: Coating for FEGSEM

Contents Retrieved from Microscopy Listserver Archives
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I use rod C-coating in a denton vacuum evaporator. The coating is smooth
and almost invisible up to 100KX magnification. I've also used a Balzers
freeze fracture machine for coating only. I deposit 2 nm of Pt and back
it with 12 nm of carbon. Then I use a backscatter detector at 10kV in the
microscope to look at only the Platinum.

A 2nmPt/10nm Carbon coated backscatter image is at:
http://wilfred.berkeley.edu/SEM-Gallery1/pages/XyellaBacteria.htm

A similar sample sputter coated with 12nm Au/Pd is at:
http://wilfred.berkeley.edu/SEM-Gallery1/pages/GrapeDiseasedPetiole7bzSeries.htm

I don't have any C-rod coated sample images on the web, but you'll have to
take my word that it looks better than sputter coating, but not as good as
the Balzer's type of coating.

We have some other coaters on our wish list from South Bay Technology, but
not the money to cover it.


\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Fri, 22 Mar 2002, Chris Jeffree wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All
}
} having acquired a FEGSEM, I am now only too well aware of the
} shortcomings of traditional sputter coatings - thick, granular, detail-
} smothering gloop obscuring ultrastructure. What do you currently
} advise as the best and most cost-effective method of obtaining
} quality coatings in the 1 to 2 nm range?
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================
}



From daemon Fri Mar 22 16:28:02 2002



From: Eaton, Tamara :      Tamara.Eaton-at-genzyme.com
Date: Fri, 22 Mar 2002 17:03:44 -0500
Subject: Another Job Opening

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

I'm looking for a person for the following position. If you are interested
please submit your resume to Req. ID # 1089 in the careers section of
{http://www.genzyme.com} http://www.genzyme.com or contact me directly.
Thank you for your help.

Description: Support Specialized cellular technology imaging needs by
maintaining a core image analysis facility. The facility contains a variety
of automated scopes with custom C++ software analysis packages for automated
scanning, and a variety of image analysis software packages for
photo-documentation or analysis. The ideal candidate will be proficient with
microscopy and image analysis applications. Experience with HTS cell based
assays would be a plus. Primary responsibility includes the imaging of
samples coming from the SCT group; cells on slides, tissue arrays, 96 well
plates for fluorescence or brightfield. Additional responsibilities include
cell based assay development mainly FISH/ISH/IHC and supporting additional
imaging opportunities.

Tamara Eaton
Genzyme Corporation
31 New York Avenue
Framingham, MA 01701
(508) 271-3211
tamara.eaton-at-genzyme.com



From daemon Fri Mar 22 17:18:33 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 22 Mar 2002 18:11:09 -0500
Subject: Gallium Milling of 3-5 Compounds

Contents Retrieved from Microscopy Listserver Archives
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You might want to check the M&M 2001 meeting proceedings. Phil Russell from North Carolina State University gave a talk about reactive ion etching processes that might well address some of your problems. His work was directed towards metals in particular, but there may be some ideas that could work for you.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Friday, March 22, 2002 8:05 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Hello Folks;

This message is to the semiconductor people that use FIB [Focused Ion Beam]
milling systems.

We use a Gallium source FIB system to electrically isolate elements in GaAs
R.F. devices in integrated circuits. There are some caveats to doing this
in that residual Ga from the beam remains behind as well as crystalline
damage to the underlying semi-insulating substrate. That means electrical
isolation between elements is not always consistent or repeatable. I plan to
attempt to improve this process by removing the residual Ga in the milled
area chemically. However, GaAs [the substrate material] etches a great deal
in solvents, acids and even D.I. water, albeit at a very slow rate in water.

Has anyone solved this problem with a specific technique that they would
like to share, or simply compare notes?

Regards,

Peter Tomic
Anadigics, Inc.
Warren, New Jersey
Failure Analysis & Analytical Services Group

Ps. Since many like to hang a good quote on their messages, let me add one
of my favorites. This is a paraphrase.

"The great thing about physics is that it works the same everywhere in the
universe, except for certain neighborhoods in New Jersey" Woody Allen


From daemon Fri Mar 22 17:23:27 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Fri, 22 Mar 2002 15:12:39 -0800
Subject: Re: Coating for FEGSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chris:

You basically have 2 choices for high resolution "coatings": A planar
magnetron type chromium coater and an ion beam sputter deposition
system. In
general, I don't like the term "coating" as it implies a thick covering
of the
sample where what you want is a thin film that won't obscure any
detail. We
offer an Ion Beam Sputter Deposition System that also offers an etching
capability. The IBS/e, is a thin film deposition system which is
designed
to improve high resolution electron microscopy imaging by depositing
ultra-thin, fine grain metal and carbon films on specimens.

Some characteristics of ion beam sputtered films:

* 5 to 8Å Cr, Ta or W films eliminate charging and increase contrast up
to
500kX
* Film quantity required is proportional to specimen surface roughness
* Films hold down fine particles
* Ir Films act as cladding on delicate specimens subject to beam damage
* 8Å Cr films can be used when doing EDS without producing X-rays above
noise
* 80Å Cr support substrates can be produced that are cohesive,
amorphous,
and smooth

Ion beam sputtered material evolves controllably and repeatable with an
energy {25eV. There is no heat or radiation artifacts to decorate
specimen
detail.

We can deposit many different metals and carbon or show you examples of
contrast enhancement on various types of specimens from our library of
micrographs.

Please contact me off line for more information or visit our website at
www.southbaytech.com and type in the keyword IBS/e.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David

Chris Jeffree wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
}
} having acquired a FEGSEM, I am now only too well aware of the
} shortcomings of traditional sputter coatings - thick, granular, detail-
} smothering gloop obscuring ultrastructure. What do you currently
} advise as the best and most cost-effective method of obtaining
} quality coatings in the 1 to 2 nm range?
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Fri Mar 22 17:25:38 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 22 Mar 2002 18:05:21 -0500
Subject: HREM samples: HOPG using sticky tape?

Contents Retrieved from Microscopy Listserver Archives
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One thing that I forgot to mention in my original post was that I first cleaved the sample and put the freshly exposed cleaved surface in the LT-wax to adhere it onto the glass slide.
-Scott

-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Friday, March 22, 2002 10:44 AM
To: 'Mark YEADON'
Cc: Microscopy (E-mail)


I have done crystalline MoS2 with tape, but in a reverse way than you describe. I used low temperature wax such as Quick-Stick from South Bay Technology to attach my sample to a glass slide. Then I used tape to peel layers away until I got a sample that was very optically transparent. Then I dissolved the LT-wax in acetone, washed it in acetone, and captured the samples onto a grid. I got a several very good samples.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Mark YEADON [mailto:m-yeadon-at-imre.org.sg]
Sent: Thursday, March 21, 2002 9:45 AM
To: 'Microscopy-at-sparc5.microscopy.com'


Dear Colleagues,

I am trying to obtain HREM samples from a block of highly-ordered pyrolitic
graphite (HOPG), 1cm2 by 2mm (bought from a supplier). I've been using the
sticky-tape technique to peel a few layers off the block, and then strip
away layer after layer of this peeled layer with more sticky tape,which I
believe is a well-known technique.

I now need to remove the sellotape - this used to be done with acetone, but
the problem is, the modern recipe for Sellotape glue seems to be no longer
acetone-soluble (I even brought some sellotape to Singapore from UK to try).
I have tried hi-purity Tetrahydrofuran (THF) and it dissolves the glue layer
beautifully, but I seem to get a thick amorphous residue on the graphite
despite many rinses.

Is anyone still using this technique? Can anyone help me?

Many thanks!

Mark

%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119260

http://www.matsci.nus.edu.sg/STAFF/Mark.html
TEL: (+65) 874 8591
FAX: (+65) 872 0785
Email: m-yeadon-at-imre.org.sg


From daemon Fri Mar 22 18:18:32 2002



From: Anna Logvinova :      alogvinova-at-buckinstitute.org
Date: Fri, 22 Mar 2002 16:12:27 -0800
Subject: Digital micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


JoAnn,

The only way we found to preserve the quality of dm3 files was to change them into jpg or tif within the Digital Micrograph program, using "export" tool in the pull-down menu; tip given by Gatan rep, who I had to ask come out here, because the final tif images were of such poor quality. That seems to preserve the quality and not collapse the files 3 times. Please contact me if you have questions.

Anna Logvinova, M.D.
Morphology Core Supervisor
Buck Institute
8001 Redwood Blvd
Novato, CA 94948
www.buckinstitute.org

(415)209-2259
alogvinova-at-buckinstitute.org


From daemon Fri Mar 22 18:25:23 2002



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Fri, 22 Mar 2002 16:11:49 -0800
Subject: Cryo EM course including HPF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everyone

This is an announcement for an International Cryo EM Course

When: June 21-29, 2002

Where: University of British Columbia, Vancouver, Canada

Organizers: Dr. Elaine Humphrey (UBC), Dr. Kent MacDonald (Berkeley),
Dr. Stan Erlandsen (Minnesota)

More information and application form
http:www.emlab.ubc.ca and follow the cryo-em links.

Please read the brochure about where to send the accommodation and
application forms.

More information about Manufacturers support and additional
International Faculty to follow soon.
Elaine

--
Dr. Elaine Humphrey
Director, Biosciences Electron Microscopy Facility
First Vice President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca
website: www.emlab.ubc.ca


From daemon Fri Mar 22 19:16:19 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 22 Mar 2002 17:11:08 -0800
Subject: Re: Sample Preparation for TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Chad

I would suggest, before spoil your time on TEM sample preparation, check
your plastic material for the compatibility with organic solvents used in
EM. You may do that just calling manufacturer or, as I did it many times,
immerse blank cell-culture unit or whatever it is into 100% Et-OH, 100%
Acetone and propylene-oxide(PO). See what happening. It's possible that
your support material may deform under some conditions but survive. I was
using some funny very expensive units with unknown plastic for primary
human retinal cells culture. It was completely dissolved in acetone but
survive in PO. Please, keep in mind: cell-culture guys sometime just don't
care so much what plastic they are using. They could easily switch to
acetate-cellulose or something like that, which is comparable with most EM
procedures. With some limitations, you could directly switch from 100%
Et-OH to the Spurr without acetone/PO. Most plastics will survive here. I
don't know what is your set-up, so it's difficult to give right
advise. There are two basic set-ups I do know. First - it's just
multi-well Petri-dish and cells are growing on the bottom of the well. In
this case I usually put microscope cover glass on the bottom and do all
procedure on the glass. Another set-up is when they used kind of the short
tube with bottom made from porous plastic (cellulose derivatives usually)
this unit supposed to be immersed into Petri-dish with cultural medium and
cells are growing inside the cell-unit on the filter. In this case I fixed
cells in the unit, go through ethanol gradient up to 100% and then cut
filter from the cell and process filter only (the unit is not tolerate
acetone/PO, but filter - yes, tolerate PO). In first scenario, instead
glass you could use special plastic called Aclar specifically designed for
such type of work. I hope it'll help. There are bunch of special
cell-growing devices on the market designed for EM, check major EM
suppliers. Good luck. Sergey

At 01:01 PM 3/22/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Mar 22 21:57:03 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 22 Mar 2002 19:49:48 -0800
Subject: Fwd: Re: Digital micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Date: Fri, 22 Mar 2002 19:49:33 -0800
} To: Anna Logvinova {alogvinova-at-buckinstitute.org}
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: Digital micrograph
}
} Anna!
}
} One very important suggestion - NEVER-EVER JPEG format "to preserve the
} quality"! You also has to change from 'signed' to 'unsigned' 2-byte
} format before exporting. If you export 'signed' it will give you 8-bit
} TIFF. Another point, when you export - you lost all scale bars
} etc. Personally, I find DM very flexible, it has all tools necessary for
} image adjusting. So, I keep most images in DM and export only for making
} final pictures for publication.
}
} Sergey
}
} At 04:12 PM 3/22/02, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Mar 22 21:58:35 2002



From: Paul Voyles :      pvoyles-at-bell-labs.com
Date: Fri, 22 Mar 2002 22:52:16 -0500
Subject: Re: digital micrograph (to TIFF)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:39 AM 3/21/2002 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Recent versions of DigitalMicrograph on Windows (after 3.4, maybe earlier)
will do this. Select "export" then "TIFF format" from the file
menu. Selecting "save real data values" from the resulting dialog will
save as 16-bit TIFF. If you don't get the dialog allowing you to chose,
hold down the "alt" key, then select export-} TIFF.

It appears that in general, when saving to non-DM formats, "export" saves
actual data values, and "save" saves whatever is on the screen.

The "Gatan Fixed Format", .gfx, is also useful for storing image data in a
format accessible to other programs. It is well-documented on the Gatan
web site at

{http://www.gatan.com/~software/guide/importexport.html}



Best wishes,
Paul

Paul Voyles
Post-doctoral MTS
Bell Laboratories
700 Mountain Ave, Rm 1D-437
Murray Hill, NJ 07974-0636
voice: (908) 582-4399
fax: (908) 582-4868



From daemon Fri Mar 22 23:35:47 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 23 Mar 2002 00:24:38 -0500
Subject: Coatings for FEGSEM applications

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Chris Jeffree wrote:
=====================================================
having acquired a FEGSEM, I am now only too well aware of the shortcomings
of traditional sputter coatings - thick, granular, detail- smothering gloop
obscuring ultrastructure. What do you currently advise as the best and
most cost-effective method of obtaining quality coatings in the 1 to 2 nm
range?
=====================================================
One of the possibilities is osmium (metal) coating with an osmium plasma
coater. It is a coating that is structureless and featureless and
reportedly completely amorphous. Information about this coater can be found
on URL
http://www.2spi.com/catalog/osmi-coat.html

One especially interesting validation of conductivity at extremely thin
coating thickness is on URL
http://www.2spi.com/catalog/osmium-plasma-coater-demonstration.html So
far as I know, one could not get that kind of BSE imaging if the coating was
more than a nm or so. So far as I know, no one has ever resolved any
"grain size" in this coating.

Also, the coating is "permanent" in the sense that being an inert precious
group metal, it won't oxidize as is the case with chromium (and into a grain
size not appreciably different from the grain size associated with a
conventional gold sputter coater).

Disclaimer: SPI Supplies distributes, provides service, and performs
"demos" for the osmium plasma coater. A working unit will be in our
exhibit booth at MSA/MSA/MAS in Quebec City.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================


From daemon Fri Mar 22 23:48:03 2002



From: max.sidorov-at-amd.com
Date: Fri, 22 Mar 2002 21:42:08 -0800
Subject: Fwd: Re: Digital micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a DM script called SaveAsFullResTiff.s which saves the image preserving its bit depth AND it also includes all annotations (if any). Should be on Gatan's web site. If not I can share.

Max

________________________________
Max Sidorov, Advanced Micro Devices



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Friday, March 22, 2002 7:50 PM
To: Microscopy-at-sparc5.microscopy.com



} Date: Fri, 22 Mar 2002 19:49:33 -0800
} To: Anna Logvinova {alogvinova-at-buckinstitute.org}
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: Digital micrograph
}
} Anna!
}
} One very important suggestion - NEVER-EVER JPEG format "to preserve the
} quality"! You also has to change from 'signed' to 'unsigned' 2-byte
} format before exporting. If you export 'signed' it will give you 8-bit
} TIFF. Another point, when you export - you lost all scale bars
} etc. Personally, I find DM very flexible, it has all tools necessary for
} image adjusting. So, I keep most images in DM and export only for making
} final pictures for publication.
}
} Sergey
}
} At 04:12 PM 3/22/02, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu







From daemon Sat Mar 23 14:18:45 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Sat, 23 Mar 2002 15:06:18 -0500
Subject: Coating for FEGSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Chris,

If you already know, or have tried this, then just ignore my post.
You may be able to do better with the equipment you have.

Depending on the coater(s) you have at your disposal, if you can
control the current and time of the coater system, you can greatly
improve the coating for high magnification work by lowering the
current setting, and running for a longer time. The lower current
slows the deposition. The deposited film turns out more uniform,
without forming "clumps". Gold-palladium targets seem to do
better than straight gold. Adjusting the time will control the
thickness. This is how we survived when we first started working
with the higher magnifications in an FEGSEM.

Regards,
Darrell

"Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk} on 03/22/2002 10:34:33 AM

Please respond to c.jeffree-at-ed.ac.uk

To: microscopy-at-sparc5.microscopy.com
cc:


Dear All

having acquired a FEGSEM, I am now only too well aware of the
shortcomings of traditional sputter coatings - thick, granular, detail-
smothering gloop obscuring ultrastructure. What do you currently
advise as the best and most cost-effective method of obtaining
quality coatings in the 1 to 2 nm range?

Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================






From daemon Sat Mar 23 15:06:45 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sat, 23 Mar 2002 14:01:48 -0700
Subject: Digital micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Be careful when converting images to JPG. JPG is a LOSSY compression. In
other words, JPG compression reduces the information in the images. In many
cases it does not matter, but if you need to conserve all information, JPG
may not be the best solution. In addition, the loss is cumulative. If you
save as JPG, open it and save it as JPG again, you incur the loss twice.

TIF is usually a much better choice.

mike

} } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Ave #300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Anna Logvinova [mailto:alogvinova-at-buckinstitute.org]
Sent: Friday, March 22, 2002 5:12 PM
To: redhair-at-leland.stanford.edu
Cc: Microscopy Listserver


JoAnn,

The only way we found to preserve the quality of dm3 files was to change
them into jpg or tif within the Digital Micrograph program, using "export"
tool in the pull-down menu; tip given by Gatan rep, who I had to ask come
out here, because the final tif images were of such poor quality. That seems
to preserve the quality and not collapse the files 3 times. Please contact
me if you have questions.

Anna Logvinova, M.D.
Morphology Core Supervisor
Buck Institute
8001 Redwood Blvd
Novato, CA 94948
www.buckinstitute.org

(415)209-2259
alogvinova-at-buckinstitute.org


From daemon Sat Mar 23 15:17:15 2002



From: Al Coritz :      Cactusgrower-at-earthlink.net
Date: Sat, 23 Mar 2002 16:11:00 -0500
Subject: Coating for FEGSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chris:


Methods like Osmium or Chromium Sputtering do produce a higher
resolution coating than you basic sputter coater. However, to address
your cost effective part of your question first; you may wish to start
by just installing a Platinum target in a standard sputter coater. This
is the most cost effective method of improving your resolution without
spending lots of money. If this method does not produce the resolution
you require, then you might consider purchasing a different coating
system.

Chris, what you really need to do first is to check out the following
authors. These are considered by many to be the leaders in the
FEGSEM/Coating field and routinely produce excellent results. By
examining their work you will get a better feel for what is the best and
have a better understanding of the sample preparation process for
FEGSEM. Then you can address the issue of cost vs. performance.

Suggested Authors to review:

Paul Walther; Stan Erlandsen; Ya Chen; Martin Muller; Rob Apkarian; Theo
Muller; Heinz Gross.


Disclaimer: EMS is in the business of marketing preparation systems for
many applications including SEM & FEGSEM.


Al Coritz, Sales Manager
Electron Microscopy Sciences
V: 215-646-1478
F: 215-646-8931
Cactusgrower-at-earthlink.net
www.emsdiasum.com


-----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
Sent: Friday, March 22, 2002 10:35 AM
To: microscopy-at-sparc5.microscopy.com


Dear All

having acquired a FEGSEM, I am now only too well aware of the
shortcomings of traditional sputter coatings - thick, granular, detail-
smothering gloop obscuring ultrastructure. What do you currently
advise as the best and most cost-effective method of obtaining
quality coatings in the 1 to 2 nm range?

Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================



From daemon Sat Mar 23 16:22:26 2002



From: zaluzec-at-microscopy.com
Date: Sat, 23 Mar 2002 16:12:34 -0600
Subject: Image File Formats (Long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Colleagues

I put together this DRAFT of a discussion on Image File formats
for a different group of users but as the current discussion
appears to headed somewhat in that direction I thought this
summary of information might be useful to the List.
The purpose of this document was directed to a discussion of
Video Imaging as related to data archiving, and remote viewing
(i.e. TelePresence Collaboratories) but some comments below are
relevant to the topic at hand.

The formats described below DO NOT include proprietary
formats like those used in the various commerical systems
we as Microanalyst's use, (...pick your vendor)
but it will at least put common nominclature on the table.


Nestor
Your Friendly Neighborhood SysOp

-------------------------------------
Excerpted From Imaging Overview- 20020313-NJZ.doc
-------------------------------------

Section 4 : Image File Formats and Compression Schemes.

Imaging data once recorded must be stored for use in the NEESgrid
Collaboratory. In
order to do this the information must ultimately be placed into a
file in some well defined format. In this section we outline
the varoius formats and compression schemes.

Lossless Data Format

These are file formats which do not mathematically change, in any
way, the numerical data within an image. A losslessly stored image
can be stored and reread and the exact same intensity at each pixel
will be recovered from which the original file was derived. . Some
files formats can compress data without loss, examples of this are
TIFF, JPEG-LS and PNG.

If any quantitative measurements of a image are to be anticipated, a
master copy of the original data set must always be archived in a
true lossless format.

If a format does not specifically state it is lossless, then you
should assume that in it are some type of compression alogrithms and
thus your data will be modified. Examples of loss of resolution due
to compression schemes in section 5 of this document.

Raw Data

A raw data format is a sequential pixel by pixel storage of the
absolute intensity of each point of an image. Raw data formats
have no compression. Raw data may be stored a number of different
ways, either as binary or as a series of ascii numbers. The format
of raw data files depends upon the program being used. Many
custom software programs have developed their own internal schemes of
storing "raw data" sometimes including tags built-in to the file
format to save metadata concerning the image.


TIFF (Tagged Image File Format)

TIFF is one of the most popular and flexible of the current public
domain raster file formats. There are no provisions in TIFF for
storing vector graphics, text annotation. TIFF is based on
file-offsets, so that it is not easily "streamable" in the way JPEG
JFIF streams are. TIFF was developed by Aldus and Microsoft Corp, and
the specification was owned by Aldus, which in turn merged with Adobe
Systems, Incorporated. Consequently, Adobe Systems now holds the
Copyright for the TIFF specification.
The current specification of TIFF can be found at
(http://partners.adobe.com/asn/developer/pdfs/tn/TIFF6.pdf)
TIFF is a lossless format, it has a compression scheme, but that
scheme does not alter the original data only how the data is stored
within the file. Not all TIFF implementation (particuliarly older
versions) completely adhere to the TIFF Version 6 specification.
You should verify which version of TIFF any program you
use has implemented as it's storage format.


PNG (Portable Network Graphics )

The Portable Network Graphics (PNG) format was designed to replace
the older and simpler GIF format and, to some extent, the much more
complex TIFF format. It's principle target is WWW based graphics,
however, PNG's compression is fully lossless--and since it supports
up to 48-bit truecolor or 16-bit grayscale--saving, restoring and
re-saving an image will not degrade its quality, unlike standard JPEG.
http://www.libpng.org/pub/png/


JPEG (Joint Photographic Experts Group)

The best known standard from JPEG is IS 10918-1 (ITU-T T.81), which
is the first of a multi-part set of standards for still image
compression. A basic version of the many features of this standard,
in association with a file format placed into the public domain by
C-Cube Microsystems (JFIF) is what most people think of as JPEG.
http://www.jpeg.org

JPEG is designed for compressing either full-color or gray-scale
images of natural, real-world scenes. It works well on photographs,
naturalistic artwork, and similar material; not so well on lettering,
simple cartoons, or line drawings. Standard JPEG is lossy
compression, designed for "static" images it can routinely achieve
10:1 -} 20:1 compression of color with little loss in "perception"
by the unaided eye, however, the compression can be varied to
preserve the maximum amount of the original image, resulting in a
very low, compression ratio. JPEG stores full color information: 24
bits/pixel (16 million colors). While JPEG is a compression scheme
it is not a true file format , JFIF is the file format. The orginal
JPEG specification includes a lossless compression scheme, but it has
been infrequently implemented in commerical software.

Repeatedly opening and re-saving a file in a JPEG format will slowly
degrade the original image quality as each "save" operation recompresses
the data. Opening a file looking at it and then closing it without
saving changes doesnot degrade the original information beyond that
done by the first "JPEGing" of the dataset.

JPEG-2000

The JPEG 2000 initiative is intended to provide a new image coding
system using state of the art compression techniques, based on the
use of wavelet technology. Its architecture should lend itself to a
wide range of uses from portable digital cameras through to its use
in advanced pre-press, medical imaging and motion. JPEG-2000 is not a
lossless compression scheme but it is claimed to be better than
standard JPEG.
http://www.elsevier.com/gej-ng/10/22/18/62/27/33/abstract.html

JPEG-LS

Lossless JPEG compression. This is a newly defined standard. It is
being embraced by the different areas of the scientific community
for continuous-tone images, ISO-14495-1/ITU-T.87. The standard is
based on the LOCO-I algorithm (LOw COmplexity LOssless COmpression
for Images) developed at Hewlett-Packard Laboratories.
(http://www.hpl.hp.com/loco/). It does not achieve high compression
ratio's values reported are low ~ 1.1/1 to 2/1. but it has a true
lossless mode.

SJPEG

Streaming JPEG, not a real standard per se. In the context of video,
it simply means that each frame of a video is processed by a JPEG
encoder, the compression can be generally chozen by the user.

MJPEG

Motion JPEG, this is the same as SJPEG. Most high end video editing
systems use this format for data storage.


MPEG (Moving Pictures Experts Group)

MPEG is the recognized standard for motion picture compression. It
uses many of the same techniques as JPEG, but adds inter-frame
compression to exploit the similarities that usually exist between
successive frames. Because of this, MPEG typically compresses a video
sequence by about a factor of three more than "M-JPEG" methods. The
disadvantages of MPEG are (1) it requires far more computation to
generate the compressed sequence (since detecting visual similarities
is hard for a computer), and (2) it's difficult to edit an MPEG
sequence on a frame-by-frame basis (since each frame is intimately
tied to the ones around it). This latter problem has made "M-JPEG"
methods rather popular for video editing products. The quality of the
MPEG is preset and has been defined by the visual perception of a
human, it is intended specifically for the entertainment community
for viewing time synced AV, it was never intended for use on data
which is to be employed in any scientific measurements.

The basic scheme is to predict motion from frame to frame in the
temporal direction, and then to use DCT's (discrete cosine
transforms) to organize the redundancy in the spatial directions.
The DCT's are done on 8x8 blocks, and the motion prediction is done
in the luminance (Y) channel on 16x16 blocks. In other words,
given the 16x16 block in the current frame that you are trying to
code, you look for a close match to that block in a previous or
future frame (there are backward prediction modes where later
frames are sent first to allow interpolating between frames).

http://mpeg.telecomitalialab.com/


MPEG-1

Designed for Video on CD's
Designed for Hardware compression/ Software Decompression
Image size 352 x 240 (rectangular)
Image size 320 x 240 (square)

MPEG encoders have Aspect ratios in the headers
but not all decoders do square pixel decoding
Real/Microsoft (No) vs Quick Time (Yes) .

The MPEG-1 codec targets a bandwidth of 1-1.5 Mbps offering VHS
quality video at CIF (352x288) resolution and 30 frames per second.
MPEG-1 requires expensive hardware for real-time encoding. While
decoding can be done in software, most implementations consume a
large fraction of a high-end processor. MPEG-1 does not offer
resolution scalability and the video quality is highly susceptible to
packet losses, due to the dependencies present in the P (predicted)
and B (bi-directionally predicted) frames. The B-frames also
introduce latency in the encode process, since encoding frame N needs
access to frame N+k, making it less suitable for video conferencing.

MPEG-2

MPEG 2 extends MPEG 1 by including support for higher resolution
video and increased audio capabilities. The targeted bit rate for
MPEG 2 is 4-15Mbits/s, providing broadcast quality full-screen video.
The MPEG 2 draft standard does cater for scalability. Three (3) types
of scalability; Signal-to-Noise Ratio (SNR), Spatial and Temporal,
and one extension (that can be used to implement scalability) Data
Partitioning, have been defined. Compared with MPEG-1, it requires
even more expensive hardware to encode and decode. It is also prone
to poor video quality in the presence of losses, for the same reasons
as MPEG-1. Both MPEG-1 and MPEG-2 are well suited to the purposes for
which they were developed. For example, MPEG-1 works very well for
playback from CD-ROM, and MPEG-2 is great for (movies) archiving
applications and for TV broadcast applications. However, for existing
computer and Internet infrastructures, MPEG-based solutions are
expensive and require bandwidth; they were not designed with the
Internet in mind.

In MPEG2 high resolution components are less preserved than low
resolution Because the high resolution details are considered less
important to they eye in the entertainment community. "i.e. they are
interested in visual quality not quantitative measurements".

MPEG-4

The intention of MPEG 4 is to provide a compression scheme suitable
for video conferencing, i.e. data rates less 64Kbits/s. MPEG4 will be
based on the segmentation of audiovisual scenes into AVOs or
"audio/visual objects" which can be multiplexed for transmission over
heterogeneous networks. The MPEG-4 framework currently being
developed focuses on a language called MSDL (MPEG-4 Syntactic
Description Language). MSDL allows applications to construct new
codecs by composing more primitive components and providing the
ability to dynamically download these components over the Internet.
This philosophy is similar to that for the multimedia APIs being
developed for Sun Microsystems Java, where it will be possible to
dynamically download codec components. This trend is also seen in
products from major vendors such as Microsoft and Netscape, where
they allow for multiple audio and video codecs to be plugged into
their real-time streaming solutions.

Other Video Image formats

NTSC (National Television Standards Committee)

An NTSC (analog) TV image has 525 horizontal lines per frame,
however, due to overscan the number typically seen by a viewer is
only 482 . These lines are scanned from left to right, and from
top to bottom. Every other line is skipped. Thus it takes two screen
scans to complete a frame: one scan for the odd-numbered horizontal
lines, and another scan for the even-numbered lines. Each half-frame
screen scan takes approximately 1/60 of a second; a complete frame is
scanned every 1/30 second. This alternate-line scanning system is
known as interlacing.

NTSC Analog Aspect Ratio = 4:3
Note: TV's have "rectangular pixels" in contrast to
computer monitors which have square pixels. !!

There is a digital equivalent of NTSC which CCD based digital Video
Cameras operate under.

Digital Equivalent of NTSC = 720 x 486 (Full Frame), however
some cameras crop this to provide a smaller image comparable to the
underscan area of a TV set and is equivalent to ~ 640 x 480.

PAL (Phase Alternation Line)

A color television signaling standard with 625 scan lines and 25
interlaced frames/second. Used in Europe/Asia/Australia
Digital Equivalent of PAL = 768 X 576(Full Frame)

SECAM (Sequential Couleur Avec Memoire )

A color television signaling standard with 625 scan lines and 25
interlaced frames/second. Used in France, the Newly Independent
States (NIS) of the former Soviet Union, and parts of the Middle East.

H.261

* targeted at teleconferencing applications mainly for use over
ISDN lines
* encoding alogrithm is similar to MPEG but incompatible with it. .
* supports 2 resolutions QCIF, CIF
* optimized for picture quality vs motion.
i.e. static images are better than moving ones.
~ approximately a constant bit rate encoding rather than
constant quality.

H.263

* targeted at low bit rate communications,
* replaces H.261 in many apps
* supports 5 resolutions: QCIF, CIF, SQCIF, 4CIF, 16CIF
* Most implementations of H.263 in hardware operated at SQCIF,
QCIF, or CIF.



H.323

H.323 is a standard that specifies the components, protocols
and procedures that provide multimedia communication services of near
real-time audio, video, and data communications over packet networks,
including Internet protocol (IP) based networks.. The video
component of H323 is given by H.261 or H.263 standard (above) while
the audio standard is G. 722, G.723.1 or G.728.
Polycomm units use the H.323 protocol to communicate with each other.

Image Format Comparison Information

Format Resolution Aspect Ratio
SQCIF 128 x 96 4/3=1.333
QCIF 176 x 144 1.222
CIF 352 x 288 1.222
4CIF 704 x 576 1.222
16CIF 1408 x 1152 1.222

Notice that nearly all CIF based formats have different aspect ratios
than video camera's, if this is not compensated for then a distortion
will be introduced into any
image stored in that format. Note that all versions of MPEG are based
upon CIF defined formats!!!

Format Bandwidth(kcps) Max FPS Resolutions Supported
H.261 384 - 2000 30 QCIF, CIF
H.263 28.8 -768 30 SQCIF, QCIF, CIF, 4CIF, 16CIF
MPEG-1 400 - 2000 30 QCIF, CIF, 4CIF
MPEG-2 1500 - 6000 30 4CIF, 16 CIF
MPEG-4 28.8 - 500 30 QCIF, CIF
JPEG N/A N/A Any. Different compressions
algorithms are available, when
compressed the data is nolonger
losseless
JPEG-LS N/A N/A Any. This is a mathematically
lossless format
TIFF
N/A N/A Any. This is a mathematically lossless format
PNG
N/A N/A Any. This is a mathematically lossless format

NTSC/PAL/ SCEAM Resolutions Compared

Vertical
Lines Active Lines Vert Res Aspec Ratio Hori Res Frame Rate
NTSC 525 484 340 4/3=1.33 330 29.94
PAL 625 575 290 4/3=1.33 425 25
SECAM 625 575 290 4/3=1.33 465 25
D-NTSC 486 486 486 1.48 720 29.94
D-NTSC* 480 480 480 4/3=1.33 640 29.94
D-PAL 576 576 576 4/3 768 25
*typically used for display purposes to keep aspect ratio correct
Note: some D-NTSC formats have a different aspect ratio, this must be
kept track of.


NTSC & HDTV Resolution

Standards Format Vertical Horizontal I/P* Aspect Ratio
NTSC VHS 525 lines 275 lines Interlace 4/3
NTSC Broadcast 525 lines 330 lines Interlace 4/3
NTSC 12" Laserdisc** 525 lines 425 lines Interlace 4/3
NTSC DVD*** 525 lines 486 lines Interlace 4/3
HDTV Broadcast 480 Pixels 720 Pixels Progressive 4/3
HDTV HD-DVD**** 1080 Pixels 1920 Pixels Interlace 16/9


Microsoft NETMEETING Video

Size Format Resolution Aspect Ratio
Large CIF 352 x 288 1.22
Medium QCIF 176 x 144 1.22
Small SQCIF 128 x 96 1.22


Notice that if an originally square image is encoded by any of the
CIF type formats then generally the aspect ratio will be changed
and the image shown by the viewing software of the Collaboratroy
will be distorted!!! This is particuliarly important
when viewing images remotely, using any real-time viewing
protocols.


From daemon Sat Mar 23 17:14:39 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 23 Mar 2002 15:13:33 -0800
Subject: Re: Coating for FEGSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Changing from Au to Au/Pd will help, but beyond
going to Chrome (which oxidizes rapidly), consider
a Pt target. Low power, longer than normal deposition
rate, and pressure of about 60-80mT works well in
a conventional sputter coater. At least for the specimens
I work with.

gary g.



At 07:34 AM 3/22/2002, you wrote:

} Dear All
}
} having acquired a FEGSEM, I am now only too well aware of the
} shortcomings of traditional sputter coatings - thick, granular, detail-
} smothering gloop obscuring ultrastructure. What do you currently
} advise as the best and most cost-effective method of obtaining
} quality coatings in the 1 to 2 nm range?
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================



From daemon Sun Mar 24 17:11:01 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 24 Mar 2002 17:50:45 -0500
Subject: Coating for FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gary Gaugler wrote:
================================
Changing from Au to Au/Pd will help, but beyond
going to Chrome (which oxidizes rapidly), consider
a Pt target. Low power, longer than normal deposition
rate, and pressure of about 60-80mT works well in
a conventional sputter coater. At least for the specimens
I work with.
================================
Has anyone ever published data showing that Au/Pd sputtering gives a smaller
grain size than Au? In the early days, e.g. early 1970's, there were some
publications showing that for vacuum evaporation, this was indeed the case,
but are there publications showing conclusively that this carries over to
sputtering?

Also, for Pt sputtering in a conventional SEM coater, while the grain size
might be slightly smaller, the longer sputtering time (everything else being
equal) for heat sensitive samples for many seems to not be as viable an
option as it might at first appear. It has been our experience working with
customers with FESEMs that the small reduction in size of Pt does not get
them into the ball park where they want to be in terms of grain size.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================








From daemon Sun Mar 24 17:31:19 2002



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Date: Sun, 24 Mar 2002 18:24:43 -0500
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Contents Retrieved from Microscopy Listserver Archives
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From daemon Sun Mar 24 18:36:34 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Sun, 24 Mar 2002 19:33:05 -0500
Subject: Video NTSC to PAL Conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;

Is anyone aware of any PC based software/hardware package that could be used
to easily convert from U.S. NTSC video standard to European PAL standards?
I have some lab. procedures and tests I'd like to send to an associate in
Europe and I'd like to not have to convert it to digital .mpg or .avi format
since the file size would be inordinately large for the period of time the
video requires. I know there are a few firms that will do the conversion if
I send the VHS video tape out but I'd love to have that capability at a
desktop.

Regards,
Peter Tomic
Group Leader
Failure Analysis & Analytical Services
Anadigics, Inc.
141 Mt. Bethel Road
Warren, New Jersey
U.S.A.


From daemon Sun Mar 24 20:14:12 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 24 Mar 2002 18:12:04 -0800
Subject: Re: Coating for FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With a magnetron sputter coater, heating is not
an issue. I coat with Au/Pd or Pt for about one
minute and get 50A of metal. Works fine from
20X to 200KX.

I'm using an Anatech Hummer VII.

gary g.


At 02:50 PM 3/24/2002, you wrote:

} Gary Gaugler wrote:
} ================================
} Changing from Au to Au/Pd will help, but beyond
} going to Chrome (which oxidizes rapidly), consider
} a Pt target. Low power, longer than normal deposition
} rate, and pressure of about 60-80mT works well in
} a conventional sputter coater. At least for the specimens
} I work with.
} ================================
} Has anyone ever published data showing that Au/Pd sputtering gives a smaller
} grain size than Au? In the early days, e.g. early 1970's, there were some
} publications showing that for vacuum evaporation, this was indeed the case,
} but are there publications showing conclusively that this carries over to
} sputtering?
}
} Also, for Pt sputtering in a conventional SEM coater, while the grain size
} might be slightly smaller, the longer sputtering time (everything else being
} equal) for heat sensitive samples for many seems to not be as viable an
} option as it might at first appear. It has been our experience working with
} customers with FESEMs that the small reduction in size of Pt does not get
} them into the ball park where they want to be in terms of grain size.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.2spi.com
} ########################
} ============================================



From daemon Sun Mar 24 20:36:40 2002



From: Al Coritz :      Cactusgrower-at-earthlink.net
Date: Sun, 24 Mar 2002 21:30:49 -0500
Subject: Job Posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Subscribers:

I am posting this position as a favor for Judy Luck, Laboratory
Manager, Virginia Commonwealth University, in Richmond, Virginia



Al Coritz, Sales Manager
Electron Microscopy Sciences
V: 215-646-1566
F: 215-523-5874
Cactusgrower-at-earthlink.net
www.emsdiasum.com





Electron Microscopy Supervisor

Pathology- Anatomic Pathology is currently seeking a full-time
Electron Microscopy Supervisor to perform special and routine
diagnostic procedures. This position will also coordinates the daily
workflow in EM laboratory to assure prompt and quality service.
Applicant must be proficient in EM procedures and techniques. BS or
BA in biological science. EMSA certification is required.



Position Information:

Perform special and routine diagnostic procedures necessary for
patient care as provided by the Electron Microscopy Lab for both
transmission and scanning electron microscopy. Coordinate the daily
work flow in the Electron Microscopy laboratory to assure prompt and
quality service. Maintain accurate records of lab tests and prepare
appropriate reports as directed by Laboratory manager.

Day Shift position; no weekends Working hours 8:00- 4:30pm. Apply on
our website: www.vcuhealth.org {http://www.vcuhealth.org/}






From daemon Mon Mar 25 02:22:53 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 25 Mar 2002 08:17:58 +0000 (GMT Standard Time)
Subject: Re: Video NTSC to PAL Conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Peter,

It is often easier for the recipient to find a dual
standard VCR to play the NTSC tape, you may need to ensure
that it is on the correct format for them (VHS, Beta, Hi8,
etc). If that is not available we used to pay about £10
(sterling) per minute for conversion.

Ron
ps our TV anoraks call NTSC `Never The Same Colour' as it
is thought to be technically inferior to PAL:-)


On Sun, 24 Mar 2002 19:33:05 -0500 Peter Tomic
{PTomic-at-anadigics.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Folks;
}
} Is anyone aware of any PC based software/hardware package that could be used
} to easily convert from U.S. NTSC video standard to European PAL standards?
} I have some lab. procedures and tests I'd like to send to an associate in
} Europe and I'd like to not have to convert it to digital .mpg or .avi format
} since the file size would be inordinately large for the period of time the
} video requires. I know there are a few firms that will do the conversion if
} I send the VHS video tape out but I'd love to have that capability at a
} desktop.
}
} Regards,
} Peter Tomic
} Group Leader
} Failure Analysis & Analytical Services
} Anadigics, Inc.
} 141 Mt. Bethel Road
} Warren, New Jersey
} U.S.A.
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Mon Mar 25 05:11:53 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 25 Mar 2002 11:03:47 +0000 (GMT Standard Time)
Subject: Reichert Ultracut E manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I could probably photocopy mine and post it if no one has
an easier method. Do you want the circuit diagrams as well?

Dave


On Fri, 22 Mar 2002 08:47:35 -0000 "Hyman, S.C."
{sch10-at-leicester.ac.uk} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi everyone,
}
} Is there any chance that someone 'out there' may have a Reichert
} Ultracut E manual that may be spared/electronically shared?
}
} TIA
}
}
} Stefan
}
}
} S.C. Hyman
} Chief Technician
} The Electron Microscope Laboratory
} Faculty of Medicine and Biological Sciences
} Adrian Building
} University of Leicester
} University Road
} Leicester
} LE1 7RH
}
} Tel. (0116) 252 3370
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Mar 25 05:14:31 2002



From: Torsten.Fregin-at-zoologie.uni-hamburg.de
Date: Mon, 25 Mar 2002 12:09:13 +0100
Subject: Re: Video NTSC to PAL Conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Peter (and everyone else),

most (new) VCRs in Germany/Europe (as the companies sell them all over Europe, in most
cases with thick handbooks in all languages) can (re)play both video standards, so if your
associate has a new VCR (or buys one for 150 €), you should not have any problems. Just
send a tape.
Vice versa (PAL to NTSC) it is a problem... Some universities have media centers, you can
get copies at low cost (~ $ 15 per tape; but that is for education purposes).

:-) Torsten


}
}
} Folks;
}
} Is anyone aware of any PC based software/hardware package that could
} be used to easily convert from U.S. NTSC video standard to European
} PAL standards? I have some lab. procedures and tests I'd like to send
} to an associate in Europe and I'd like to not have to convert it to
} digital .mpg or .avi format since the file size would be inordinately
} large for the period of time the video requires. I know there are a
} few firms that will do the conversion if I send the VHS video tape out
} but I'd love to have that capability at a desktop.
}
} Regards,
} Peter Tomic
} Group Leader
} Failure Analysis & Analytical Services
} Anadigics, Inc.
} 141 Mt. Bethel Road
} Warren, New Jersey
} U.S.A.
}




Torsten Fregin

Universität Hamburg - Zoologisches Institut
Abt. Neurophysiologie
AG Wiese - Raum 413
Martin-Luther-King-Platz 3
20146 Hamburg, Germany
Telefon *49-(0)40-42838-3931
Fax *49-(0)40-42838-3937
eMail Torsten.Fregin-at-zoologie.uni-hamburg.de
oder TorstenFregin-at-gmx.de





From daemon Mon Mar 25 06:47:26 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Mon, 25 Mar 2002 07:39:30 -0500
Subject: EM Facility Microscopist Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



ELECTRON MICROSCOPIST

Electron Microscopy Facility is seeking to fill a fulltime, 12 month unclassified
staff position as ELECTRON MICROSCOPIST. The successful candidate will
serve as an assistant to the Electron Microscopy Facility Supervisor and help
oversee the facility. The EMF houses two SEMs, two TEMs, an EDS system,
a laser scanning confocal microscope, light microscopes, and imaging
workstations.

Duties include maintaining and troubleshooting the EM’s and specimen
preparation equipment, ordering routine supplies, maintaining records on
equipment usage, administering the EM Facility’s computer systems,
providing for the routine maintenance of the instrumentation infrastructure,
and overseeing the laboratory technicians. In addition, the person will
collaborate with and/or assist faculty and student researchers in advanced
microscopy techniques, and help teach microscopy and digital imaging
methods.

A masters degree or equivalent experience and a strong background in
electron microscopy (TEM and/or SEM) are required. The applicant should
have experience in various aspects of sample preparation for biological EM
including fixation, embedding, ultrathin sectioning, staining and darkroom
procedures. Strong computer skills are desirable.

For more information please visit Miami University’s EM Facility website at
http://www.emf.muohio.edu/.

Interested individuals should send a cover letter, a current curriculum vitae,
representative micrographs (if available), a statement of any relevant research
and teaching interests related to microscopy, and have three letters of
recommendation forwarded to: Department of Zoology; Attention: Dr. Richard
E. Edelmann, EM Facility Supervisor; Miami University, Oxford, OH 45056
Phone: 513-529-5712. Email: EdelmaRE-at-muohio.edu

The search committee will begin reviewing applications on April 15th, 2002.

Miami University is an Affirmative Action/Equal Employment Opportunity
employer. Women, minorities, veterans, and persons with disabilities are
encouraged to apply.





Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Mon Mar 25 07:21:34 2002



From: Tom Budd :      tbudd-at-stlawu.edu
Date: Mon, 25 Mar 2002 08:14:03 -0500
Subject: position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


MICROSCOPIST

Search continued

St. Lawrence University seeks a MICROSCOPY TECHNICIAN. This is a
full-time, 12 month academic support staff position. A bachelor’s
degree in science (preferably in biology) is required. A master’s
degree and/or experience with confocal microscopy as well as electron
microscopy (TEM and/or SEM) are preferred. The successful candidate
should show evidence of mechanical and laboratory aptitude, computer
experience, a desire to learn and teach new methods, and a positive work
ethic.

The successful candidate will help oversee a developing
interdisciplinary, multi-user microscopy/imagery center, will assist
faculty and student researchers in advanced microscopy techniques, help
teach microscopy methods and provide for the routine maintenance of the
instrumentation infrastructure. The major instruments at the facility
are on service contracts and the candidate will be expected to develop
good working relationships with the professional service personnel. The
facility is housed in the biology department and the successful
candidate will also serve other science departments.

Applicants should send a letter of application, a current curriculum
vitae (including references), if appropriate, a statement of any
relevant research and teaching interests related to microscopy, and have
three letters of recommendation forwarded to Dr. T. Budd, Biology
Department, St. Lawrence University, Romoda Drive, Canton, NY 13617.
The search committee will review applications until the position is
filled.

St. Lawrence University, chartered in 1856, is an independent, private,
non-denominational university whose mission is to provide an inspiring
and demanding undergraduate education in the liberal arts to students
selected for their seriousness of purpose and intellectual promise. The
University's 2100 students come from 35 U. S. states and 21 countries.
Located halfway between the high peaks of the Adirondack Mountains and
the national capital of Canada, Ottawa, the University provides
unparalleled access to outdoor recreation and international social and
cultural opportunities. For more information please visit SLU’s homepage
at http://www.stlawu.edu/resources/job.html. St. Lawrence University is
an Affirmative Action/Equal Employment Opportunity employer. Women,
minorities, veterans, and persons with disabilities are encouraged to
apply.


--
Dr. T. Budd
Chair of Biology
St. Lawrence University
Canton, NY 13617
Phone = 315-229-5640
Fax = 315-229-7429
E-mail = tbudd-at-stlawu.edu




From daemon Mon Mar 25 08:00:11 2002



From: nleddy :      nleddy-at-tcd.ie (by way of MicroscopyListserver)
Date: Mon, 25 Mar 2002 07:50:21 -0600
Subject: RE: Video NTSC to PAL Conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Peter,

You could try tsunami mpeg encoder shareware downloadable from www.tmpgenc.net
it is the best softeare encoder in existence, allowinf you to change frame
rate from pal 25fps to ntsc 29.97 / 23.97 (film), it will also allow you to
resize the output frame from pal 352x288 to ntsc 352x240.

I have tested all mpeg software encoders and this is by far the best.

Regards
Neal


From daemon Mon Mar 25 08:00:11 2002



From: Briem . :      briemeng-at-hotmail.com
Date: Mon, 25 Mar 2002 07:52:25 -0600
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




_________________________________________________________________
MSN Photos is the easiest way to share and print your photos:
http://photos.msn.com/support/worldwide.aspx



From daemon Mon Mar 25 08:05:21 2002



From: nleddy :      nleddy-at-tcd.ie
Date: Mon, 25 Mar 2002 13:59:30 +0000
Subject: NTSC to PAL etc,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Peter,

You could try tsunami mpeg encoder shareware downloadable from www.tmpgenc.net
it is the best softeare encoder in existence, allowinf you to change frame
rate from pal 25fps to ntsc 29.97 / 23.97 (film), it will also allow you to
resize the output frame from pal 352x288 to ntsc 352x240.

I have tested all mpeg software encoders and this is by far the best.

Regards
Neal



From daemon Mon Mar 25 09:42:06 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 25 Mar 2002 08:38:44 -0700
Subject: Re: Video NTSC to PAL Conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thorsten,

are you sure about NTSC and PAL VCR's? Or are you talking about PAL/SECAM
VCRs? PAL/SECAM would make more sense, because the two signals use the same
bandwidth with a different scheme to transfer the color information. NTSC
uses a different bandwidth. Also, PAL and SECAM are both used in Europe
(Secam: France, PAL: rest of Europe), while NTSC is used in the US.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "Torsten.Fregin-at-zoologie.uni-hamburg.de"-at-sparc5.microscopy.com
[mailto:"Torsten.Fregin-at-zoologie.uni-hamburg.de"-at-sparc5.microscopy.com]
Sent: Monday, March 25, 2002 4:09 AM
To: Microscopy-at-sparc5.microscopy.com; Peter Tomic


Hi Peter (and everyone else),

most (new) VCRs in Germany/Europe (as the companies sell them all over
Europe, in most
cases with thick handbooks in all languages) can (re)play both video
standards, so if your
associate has a new VCR (or buys one for 150 EUR), you should not have any
problems. Just
send a tape.
Vice versa (PAL to NTSC) it is a problem... Some universities have media
centers, you can
get copies at low cost (~ $ 15 per tape; but that is for education
purposes).

:-) Torsten


}
}
} Folks;
}
} Is anyone aware of any PC based software/hardware package that could
} be used to easily convert from U.S. NTSC video standard to European
} PAL standards? I have some lab. procedures and tests I'd like to send
} to an associate in Europe and I'd like to not have to convert it to
} digital .mpg or .avi format since the file size would be inordinately
} large for the period of time the video requires. I know there are a
} few firms that will do the conversion if I send the VHS video tape out
} but I'd love to have that capability at a desktop.
}
} Regards,
} Peter Tomic
} Group Leader
} Failure Analysis & Analytical Services
} Anadigics, Inc.
} 141 Mt. Bethel Road
} Warren, New Jersey
} U.S.A.
}




Torsten Fregin

Universität Hamburg - Zoologisches Institut
Abt. Neurophysiologie
AG Wiese - Raum 413
Martin-Luther-King-Platz 3
20146 Hamburg, Germany
Telefon *49-(0)40-42838-3931
Fax *49-(0)40-42838-3937
eMail Torsten.Fregin-at-zoologie.uni-hamburg.de
oder TorstenFregin-at-gmx.de





From daemon Mon Mar 25 10:18:08 2002



From: Blackwood, Andrew :      ablackwood-at-2spi.com
Date: Mon, 25 Mar 2002 10:59:01 -0500
Subject: Cryo Stage SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Blackwood, Andrew * EMC.Ver #3.1a ] --

25 March 2002

Hi Listers:

Jim Romanow asked about the availability of a cryo-stage equipped SEM within
driving distance of Connecticut. The Structure Probe laboratory in West
Chester, PA has a JEOL 840 equipped with a Hexland cryo-stage and, perhaps
more important, a couple of microscopists with experience in operating the
system. Whether West Chester is within driving distance of Connecticut is
open to some discussion; I used to do it every week :-)

Disclaimer: Structure Probe is in the business of providing microscopy
services, including cryo-SEM. We have an obvious interest in promoting the
use of our equipment.

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
P.O. Box 656
West Chester, PA 19381-0656
Ph: 1 610 436 5400 X108
FAX: 1 610 436 5755
e-mail: ablackwood-at-2spi.com
WWW: http://www.2spi.com




From daemon Mon Mar 25 13:19:14 2002



From: Microscopy-request-at-sparc5.microscopy.com
Date: Mon, 25 Mar 2002 13:11:40 -0600
Subject: NTSC to PAL etc,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Peter,

You could try tsunami mpeg encoder shareware downloadable from
www.tmpgenc.net
it is the best softeare encoder in existence, allowinf you to change frame
rate from pal 25fps to ntsc 29.97 / 23.97 (film), it will also allow you to

resize the output frame from pal 352x288 to ntsc 352x240.

I have tested all mpeg software encoders and this is by far the best.

Regards
Neal






From daemon Mon Mar 25 13:19:15 2002



From: Russell Spear :      rzs-at-plantpath.wisc.edu
Date: Mon, 25 Mar 2002 13:11:02 CST
Subject: large coverslip material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are in need of a source of material for making coverslips approx 65 x
75 mm, approx. #1 thickness. Does anyone have any leads?

Thanks.

Russ Spear
Russell N. Spear
Sr. Research Specialist
Dept. of Plant Pathology
Univ. of Wisconsin-Madison

RZS-at-plantpath.wisc.edu
Phone 608 263-2093
Fax 608 263-2626


From daemon Mon Mar 25 14:34:16 2002



From: Chad Parish :      chad_parish-at-hotmail.com (by way of
Date: Mon, 25 Mar 2002 14:22:59 -0600
Subject: Re: TEM -- problem with magnetic foils -- thanks!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who replied to my post!

I found your advice very helpful. Using your advice, I was able to
get a specimen to the proper eucentric height and shoot a ZADP. I
compared the lattice parameter measured from TEM to my XRD-measured
lattice parameter and found them within about ~3% (2.95 to 2.87
angstroms). Your advice works great!

Our scope is a JEOL 200CX -- early 1980's vintage -- and I wasn't
able to find an objective lens current readout, so I had to go by the
locations of the focus knobs, rather than an actual amp readout.
Does anyone know of potential pitfalls therein?

Again, thanks to all. I'm sure I'll turn to you folks again the next
time I need help.


Chad Parish
Graduate Student
Material Science and Engineering
University of Pittsburgh

_________________________________________________________________
MSN Photos is the easiest way to share and print your photos:
http://photos.msn.com/support/worldwide.aspx


From daemon Tue Mar 26 01:14:26 2002



From: Ian Lamswood :      Ian_Lamswood-at-compuserve.com
Date: Tue, 26 Mar 2002 02:00:05 -0500
Subject: Reichert Ultracut E manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear David,
We will send you a copy of the Ultracut E manual directly.
Regards,

Ian Lamswood
Marketing Manager
EM Products
Leica Microsystems, Vienna


From daemon Tue Mar 26 04:17:16 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 26 Mar 2002 10:08:28 +0000 (GMT Standard Time)
Subject: Reichert Ultracut E manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank-you. Please send it to SC Hyman only.

Dave


On Tue, 26 Mar 2002 02:00:05 -0500 Ian Lamswood
{Ian_Lamswood-at-compuserve.com} wrote:

}
} Dear David,
} We will send you a copy of the Ultracut E manual directly.
} Regards,
}
} Ian Lamswood
} Marketing Manager
} EM Products
} Leica Microsystems, Vienna

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Mar 26 04:27:55 2002



From: GALLEN WANG :      wang8308-at-ms19.hinet.net (by way of
Date: Tue, 26 Mar 2002 08:35:06 -0600
Subject: HMDS information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Russell
No 1 coverglassses are made by Chance propper in sizes to at
least 51 x 64mm.
try
Chance Propper Ltd 
P O Box 53
Spon Lane South
Smethwick 
West Midlands
United Kingdom
B66 1NZ
Tel: +44 (0) 121 553 5551
Fax: +44 (0) 121 525 0139
Your contact at Chance Propper Ltd :
Mr Michael Williamson, General Manager
Chris


} From: "Russell Spear" {rzs-at-plantpath.wisc.edu}
Organization: University of Wisconsin
To: microscopy-at-sparc5.microscopy.com
Date sent: Mon, 25 Mar 2002 13:11:02 CST


??
Dear Sir,
I am introduced to you by Mr. Marcum of Polysciences inc., I hear
that maybe you have some experieces in the HMDS application.
We have bought some bottles to test from Polysciences, but we have
found the HMDS is no good for plant tissues, due to it will be
shrinked after immersed in HMDS. If you have experience in this
problem, could you give me some suggestion?
Thanks and Best Regards,
Lightech Technology Corp.,
Gallen Wang
Tel# 886-2-89612317
Fax# 886-2-89612353


From daemon Tue Mar 26 08:55:30 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 26 Mar 2002 14:53:56 +0000 (GMT Standard Time)
Subject: Re: TEM -- problem with magnetic foils -- thanks!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Chad,

In the right hand door of your 200CX desk there should be a point to
monitor the lens currents. At the bottom left side you should see a
set of test points labelled ' For Check' and listing CL to PL with 0V at
the bottom. Put a voltmeter between OL and 0V for the Objective lens
setting.

Good luck,
Ron

On Mon, 25 Mar 2002, Chad Parish wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------
}
}
} Thanks to all who replied to my post!
}
} } } I found your advice very helpful. Using your advice, I was able to
} } } get a specimen to the proper eucentric height and shoot a ZADP. I
} } } compared the lattice parameter measured from TEM to my XRD-measured
} } } lattice parameter and found them within about ~3% (2.95 to 2.87
} } } angstroms). Your advice works great!
} } }
} } } Our scope is a JEOL 200CX -- early 1980's vintage -- and I wasn't
} } } able to find an objective lens current readout, so I had to go by the
} } } locations of the focus knobs, rather than an actual amp readout.
} } } Does anyone know of potential pitfalls therein?
} } }
} } } Again, thanks to all. I'm sure I'll turn to you folks again the next
} } } time I need help.
} } }
} } }
} } } Chad Parish
} } } Graduate Student
} } } Material Science and Engineering
} } } University of Pittsburgh
} } }
} } } _________________________________________________________________
} } } MSN Photos is the easiest way to share and print your photos:
} } } http://photos.msn.com/support/worldwide.aspx
} } }
} } }
} }
} } ===========================================================================
} } Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
} } Department of Materials, phone +44 (0) 1865 273701
} } University of Oxford, fax +44 (0) 1865 283333
} } Parks Road.
} } Oxford. OX1 3PH. UK.
} } ============================================================================
} }

--- End Forwarded Message ---


----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Mar 26 09:35:07 2002



From: AP Alves de Matos :      apamatos-at-oninet.pt
Date: Tue, 26 Mar 2002 15:29:13 -0000
Subject: TEM cryosections, X-Ray mycroanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Collegues

I need to do in the future some observations in cryofixed biological
specimens. In the past I have done it for immunocytochemistry, and used a
fixation step and cryoprotectant.
In this case, the sections are to be subjected to X-ray microanalysis and
fixation steps are to be avoided in order to prevent diffusion of soluble
compounds.

As far as I was able to inquire, it is possible to transfer such sections to
the microscope and freeze-dry them in the microscope itself. However I do
not have specific details on the procedure, and do not know if it is
possible to do it without special equipment.

Since I will be able to ask for new equipment till the end of the week, can
anyone give-me an idea of a pratical (if possible simple) procedure to do
this, indicating required equipment and special problems to look for?

Thanks in advance

Dr. A.P. Alves de Matos
Faculty of Dental Medicine, Lisbon University
(Biologist, Ph. D.)
apamatos-at-oninet.pt




From daemon Tue Mar 26 11:28:57 2002



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Tue, 26 Mar 2002 12:01:26 -0500
Subject: Thank you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you from Greg Shenk to all who responded regarding his request for a
cryoSEM facility in the Northeast.

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
Storrs, CT 06269-2242
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Tue Mar 26 12:59:24 2002



From: Jeff Stewart :      jeff-at-metallography.com
Date: Tue, 26 Mar 2002 13:52:15 -0500
Subject: International Metallographic Contest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The annual International Metallographic Contest and Exhibit co-sponsored since
1972 by the International Metallographic
Society and ASM International will be held in conjunction with the 35th annual
convention and technical meeting of the
IMS in Quebec City during the M&M '02 festivities this August. There are 12
categories of competition. Best in show receives $3000 and the prestigious
Jaquet-Lucas Award. Deadline for entries is July 22. For additional information
including rules, tips for creating a winning entry, judging guidelines, and
examples of winning entries contact me or visit
http://www.metallography.com/ims/contest.htm.

Jeff Stewart
International Metallographic Contest Chair
Metallographic Laboratory Manager
Stern-Leach Co.
49 Pearl Street
Attleboro, MA 02703 USA
Phone: 508-222-7400 extension 1329
FAX: 508-699-4030





From daemon Tue Mar 26 13:40:49 2002



From: David Spector :      spector-at-cshl.org
Date: Tue, 26 Mar 2002 14:34:21 -0500
Subject: Petri Dishes with Gridded Coverslip Bottoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I need to repeatedly image cells growing on a coverslip in medium
using an inverted fluorescence microscope. Does anyone know of 35 mm
Petri dishes that have a gridded coverslip as their bottom or of
plastic dishes that don't autofluoresce?

Thanks,

David Spector
--
Dr. David L. Spector
Cold Spring Harbor Laboratory
One Bungtown Road
Cold Spring Harbor, New York 11724
Tel. (516) 367-8456
Fax (516) 367-8876


From daemon Tue Mar 26 22:23:31 2002



From: peter ingram :      p.ingram-at-cellbio.duke.edu (by way of
Date: Tue, 26 Mar 2002 22:11:47 -0600
Subject: Re: TEM cryosections, X-Ray mycroanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dar Colleague:

There are several books available describing what you need. I
recommend those by Alice Warley (Biological X-ray Microanalysis -
Portland Press 1997), Pat Echlin (Low Temperature Microscopy &
Analysis - Plenum 1992) and our book Peter Ingram et al (Biological
Applications of Microprobe Analysis - Academic Press 1999). I also
refer you to the work of Andrew Somlyo et al., Brian Andrews and
Richard Leapman et al. as well as Maria Wendt-Galitelli.

The short answer to your question is that you CAN do it the
way you suggest in the microscope but you had better have a cold
stage and a very clean, readily bakeout-able microscope with a good
cold trap!

Good luck!

Peter

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Peter Ingram
Sr. Physicist
Adj. Professor of Pathology,
Duke University Medical Center
Box 930319
DURHAM
NC 27708-0319

Tel: (919) 660-2695
Fax: (919) 660-2671

Website: http://152.3.167.174/AEM_LAB.html


From daemon Wed Mar 27 01:04:01 2002



From: Agneta =?iso-8859-1?Q?=D6stberg?= :      agneta.ostberg-at-sandvik.com
Date: Wed, 27 Mar 2002 07:54:56 +0100
Subject: LM - magnetic etching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have seen references about magnetic etching of ferrite-austenitic
stainless steels with a colloidal suspension of Fe(subscript: 3)O
(subscript: 4). Do any of you have experience of this technique? From where
can the etchant be purchased?

Agneta Östberg
AB Sandvik Steel
SE-811 81 Sandviken
Sweden

phone: +46 26 264311
fax: +46 26 264310
e-mail: agneta.ostberg-at-sandvik.com



From daemon Wed Mar 27 05:20:05 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Wed, 27 Mar 2002 13:07:16 +0200
Subject: Filters fort dust particle collection - ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

There was a discussion related to filters. Unfortunately I did not follow
it. We have a student interested to do dust collection at remote areas here
in Botswana and is looking for a filter with a smooth surface that is
durable. The filter must also be beam-stable since we want to do EDS
particle analysis afterward. Some filters are treated with sulphur to
reduce static charges. We will be interested in sulphur particles as well.

Please help

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana
{ {...OLE_Obj...} }

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}




From daemon Wed Mar 27 05:49:23 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 27 Mar 2002 06:47:13 -0500
Subject: HMDS information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gallen;

Yes, HMDS is used in our manufacturing process but not as a fixative for
biological materials. We make GaAs radio frequency integrated circuits and
it is used in a procedure to thin wafers. I have seen postings on this
listserver discussing it but not in a context even remotely close to our
application of it. The "MSDS" sheets may give you some details of its'
properties. We are principally a group of physicists, electrical engineers
and material scientists.

Regards,
Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: GALLEN WANG [mailto:wang8308-at-ms19.hinet.net]
Sent: Tuesday, March 26, 2002 9:35 AM
To: Microscopy-at-sparc5.microscopy.com


??
Dear Sir,
I am introduced to you by Mr. Marcum of Polysciences inc., I hear
that maybe you have some experieces in the HMDS application.
We have bought some bottles to test from Polysciences, but we have
found the HMDS is no good for plant tissues, due to it will be
shrinked after immersed in HMDS. If you have experience in this
problem, could you give me some suggestion?
Thanks and Best Regards,
Lightech Technology Corp.,
Gallen Wang
Tel# 886-2-89612317
Fax# 886-2-89612353


From daemon Wed Mar 27 07:06:44 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 27 Mar 2002 12:59:17 +0000 (GMT Standard Time)
Subject: Re: HMDS information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I saw a paper in the 1980's which concluded that HMDS was
suitable for many applications but for the fine hairs on
plants, CPD was better.

Dave


On Tue, 26 Mar 2002 08:35:06 -0600 GALLEN WANG
{wang8308-at-ms19.hinet.net} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} ??
} Dear Sir,
} I am introduced to you by Mr. Marcum of Polysciences inc., I hear
} that maybe you have some experieces in the HMDS application.
} We have bought some bottles to test from Polysciences, but we have
} found the HMDS is no good for plant tissues, due to it will be
} shrinked after immersed in HMDS. If you have experience in this
} problem, could you give me some suggestion?
} Thanks and Best Regards,
} Lightech Technology Corp.,
} Gallen Wang
} Tel# 886-2-89612317
} Fax# 886-2-89612353
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Mar 27 07:06:44 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Wed, 27 Mar 2002 07:59:24 -0500
Subject: Re: HMDS information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That has been my experience as well. Some plants will work and others will not. I have not found a pattern to help determine which will and will not work. Consequently I either process plant tissue via standard methods. If I need a more rapid protocol, I immerse the fresh tissue in a couple of changes of 100% methanol and critical point dry out of methanol. Seems to work well and even better than conventional methods for many species though for larger tissues I extend the number of changes and time between.

reference:

C. Neinhuis & G. Edelmann. Methanol as a rapid fixative for the investigation of plant surfaces by SEM. Journal of Microscopy. Vol 184, Pt 1, October 1996. pp 14-16

Scott Whittaker
SEM Lab Manager
Smithsonian Institution
PO Box 37012 MRC104
National Museum of Natural History
Washington DC 20013-7012
202-357-1651


} } } "GALLEN WANG" (by way ofMicroscopyListserver) {wang8308-at-ms19.hinet.net} 03/26/02 09:35AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


??
Dear Sir,
I am introduced to you by Mr. Marcum of Polysciences inc., I hear
that maybe you have some experieces in the HMDS application.
We have bought some bottles to test from Polysciences, but we have
found the HMDS is no good for plant tissues, due to it will be
shrinked after immersed in HMDS. If you have experience in this
problem, could you give me some suggestion?
Thanks and Best Regards,
Lightech Technology Corp.,
Gallen Wang
Tel# 886-2-89612317
Fax# 886-2-89612353




From daemon Wed Mar 27 08:47:52 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Wed, 27 Mar 2002 09:39:27 -0500
Subject: EM Facility Microscopist Position - Clarification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Clarification: This is a *permanent*, fulltime, 12-month/year unclassif=
ied
staff position as ELECTRON MICROSCOPIST.


( 12-month/year vs 9-month/year )


{color} {param} 0100,0100,0100 {/param} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D


{/color} ELECTRON MICROSCOPIST


Electron Microscopy Facility is seeking to fill a permanent, fulltime, 12=
-
month/year unclassified staff position as ELECTRON MICROSCOPIST. The
successful candidate will serve as an assistant to the Electron Microscopy=

Facility Supervisor and help oversee the facility. The EMF houses two SEM=
s,
two TEMs, an EDS system, a laser scanning confocal microscope, light
microscopes, and imaging workstations.


Duties include maintaining and troubleshooting the EM=92s and specimen

preparation equipment, ordering routine supplies, maintaining records on

equipment usage, administering the EM Facility=92s computer systems,

providing for the routine maintenance of the instrumentation infrastructur=
e,

and overseeing the laboratory technicians. In addition, the person will

collaborate with and/or assist faculty and student researchers in advanced=


microscopy techniques, and help teach microscopy and digital imaging

methods.


A masters degree or equivalent experience and a strong background in

electron microscopy (TEM and/or SEM) are required. The applicant should

have experience in various aspects of sample preparation for biological EM=


including fixation, embedding, ultrathin sectioning, staining and darkroom=


procedures. Strong computer skills are desirable.


For more information please visit Miami University=92s EM Facility website=
at

http://www.emf.muohio.edu/.


Interested individuals should send a cover letter, a current curriculum vi=
tae,

representative micrographs (if available), a statement of any relevant res=
earch

and teaching interests related to microscopy, and have three letters of

recommendation forwarded to: Department of Zoology; Attention: Dr. Richar=
d

E. Edelmann, EM Facility Supervisor; Miami University, Oxford, OH 45056

Phone: 513-529-5712. Email: EdelmaRE-at-muohio.edu


The search committee will begin reviewing applications on April 15th, 2002=
.


Miami University is an Affirmative Action/Equal Employment Opportunity

employer. Women, minorities, veterans, and persons with disabilities are

encouraged to apply.




Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Wed Mar 27 10:06:38 2002



From: Antonio Mendez Vilas :      amvilas-at-unex.es
Date: Wed Mar 27 16:58:12 2002
Subject: Call for Paper for Book Series on Science and Technology of Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear colleagues

In the very next future, FORMATEX, a technological organization located in Badajoz (Spain), will edit a series of books on the science and technology of Microscopy, as well as on educational applications. Also a website will be developed containing all the information and the Call for Paper for this edition. The book will be edited in a citeable form (ISBN) and 100 preprints will be sent to authors. The corresponding fees will be about 240 EUR (1EUR $ = 0.86 US$ aprox.).

If you are interested in receive detailed information on this edition, please contact us through formatexmicro-at-mixmail.com, or directly to the editor:

A.Mendez Vilas
Physics Department
University of Extremadura
Avda. de Elvas s/n
06071 Badajoz
SPAIN
E-mail: amvilas-at-unex.es

Hope to hear from you soon.

J.A.Mesa Gonzalez
FORMATEX Secretary



From daemon Wed Mar 27 10:10:02 2002



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Wed Mar 27 16:58:12 2002
Subject: Call for Paper for Book Series on Science and Technology of Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id KAA22989
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From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Wed, 27 Mar 2002 11:04:41 -0500
Subject: QuartzPCI database help requested

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
{H3YDCMD2} ; Wed, 27 Mar 2002 11:04:48 -0500
Message-ID: {F5EE4EE4FDDCD51184D40002A58F1A4436C107-at-RIDMSG01}
To: Microscopy-at-sparc5.microscopy.com


I was in the process of printing the results of a query when my PC
locked.. froze.. crashed, -at-#$%&!!!, etc...... I was forced to reboot. The
nature of my system failure has not been determined: older PC run windows
95, several applications were open, using a dbase shared on a network
server, printing to a network printer, in short, probably not an issue
related to the PCI application. After scan disk worked it's magic I
attempted to open the database. Unfortunately I encountered a problem with
a flashing PCI "open database error" window: Could not open database -
index is out of date. I was unable to open the database. I would
appreciate any advice, preferably good, regarding how to correct a database
error of this type.

FYI This is the only database that seems to be affected, so it does
not appear to by an application error. Attempts to open the database from
several PC's produced the same results. PCI Quartz workgroup version 5.10
build 4207



From daemon Wed Mar 27 10:32:52 2002



From: gary.m.brown-at-exxonmobil.com
Date: Wed, 27 Mar 2002 10:26:56 -0600
Subject: ACS Symposium on Imaging and Spectroscopic Techniques for Polymer Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The American Chemical Society is organizing a symposium on Imaging and
Spectroscopic Techniques for Polymer Systems at its Fall Meeting in Boston
- 8/18/02. The symposium will cover a wide range of imaging and
spectroscopic techniques for the characterization of polymer
microstructures including: Optical techniques (IR, Raman, Fluorescence,
Vibrational NSOM) - Electron Beam Techniques (Low Voltage SEM, Variable
Pressure SEM, TEM, Energy Filtering, EELS, Tomography) - Absorption
Spectroscopy - Scanning Transmission X-ray Microscopy - Scanning Probe
Methods - Surface Sensitive Techniques (ToF-SIMS imaging, XPS imaging) -
NMR imaging - and the coupling of any of these characterization techniques
to observe in-situ processes, polymer growth, deformation, etc. This would
be a symposium of general interest to any microscopist in the polymers and
biopolymers area.
If interested in participating and submitting an abstract please go to:
http://oasys.acs.org/oasys.htm for instructions and additional information.
The deadline is Friday March 29th.





From daemon Wed Mar 27 10:52:29 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 27 Mar 2002 16:46:04 +0000 (GMT Standard Time)
Subject: Re: HMDS information II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The paper on HMDS (lent to an undergraduate) was by Bray et
al. (1993).

Dave


On Tue, 26 Mar 2002 08:35:06 -0600 GALLEN WANG
{wang8308-at-ms19.hinet.net} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} ??
} Dear Sir,
} I am introduced to you by Mr. Marcum of Polysciences inc., I hear
} that maybe you have some experieces in the HMDS application.
} We have bought some bottles to test from Polysciences, but we have
} found the HMDS is no good for plant tissues, due to it will be
} shrinked after immersed in HMDS. If you have experience in this
} problem, could you give me some suggestion?
} Thanks and Best Regards,
} Lightech Technology Corp.,
} Gallen Wang
} Tel# 886-2-89612317
} Fax# 886-2-89612353
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Mar 27 11:00:13 2002



From: Antonio Mendez Vilas :      amvilas-at-unex.es
Date: Wed Mar 27 17:54:48 2002
Subject: Call for Paper for Book Series on Science and Technology of Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear colleagues

In the very next future, FORMATEX, a technological organization located in Badajoz (Spain), will edit a series of books on the science and technology of Microscopy, as well as on educational applications. Also a website will be developed containing all the information and the Call for Paper for this edition. The book will be edited in a citeable form (ISBN) and 100 preprints will be sent to authors. The corresponding fees will be about 240 EUR (1EUR $ = 0.86 US$ aprox.).

If you are interested in receive detailed information on this edition, please contact us through formatexmicro-at-mixmail.com, or directly to the editor:

A.Mendez Vilas
Physics Department
University of Extremadura
Avda. de Elvas s/n
06071 Badajoz
SPAIN
E-mail: amvilas-at-unex.es

Hope to hear from you soon.

J.A.Mesa Gonzalez
FORMATEX Secretary








From daemon Wed Mar 27 11:28:19 2002



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Wed, 27 Mar 2002 11:21:59 -0600
Subject: converting digital camera to microscope

Contents Retrieved from Microscopy Listserver Archives
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X-Mailer: QUALCOMM Windows Eudora Version 5.0.1


Hi All,
I have a question on behalf of colleague of mine who is considering his
options for adding a digital camera to his Zeiss Axiovert and stereo
microscopes. He has heard/read of adapting a Nikon Coolpix for this use,
using a special adapter. Is there anyone out there who can offer advice as
to whether this works well and how it works?
Thanks in advance for your advice,
Kristen

Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011

515-294-8854
kalen-at-iastate.edu
www.baumlab.org




From daemon Wed Mar 27 12:08:20 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 27 Mar 2002 12:02:27 -0600
Subject: Re: Coating for FEGSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Au-Pd is better than plain Au for FESEM, in my experience, but Iridium is
better than either one.
We have 2 Ir sputterers, one is an magnetron planar type (Emitech K-575) w/
an Ir target and
the other is an ion-beam sputter coater (an ancient version of the SouthBay
Tech IBS/e) with an
Ir target. The lab favorite seems to be ion beam coater as the techs think
it gives a "better"
coating for working at or above 100KX. We are a semiconductor house, so we
routinely work at
mags of 100KX to 300KX. This is not quantitative date, just people's
preferences.
I don't like chromium coatings due to the oxidation problem.
DISCLAIMER: all opinions are strictly my own, and I have no interest,
financial or otherwise,
in either Emitech or SouthBay Technologies. I just buy their stuff and use
it.

Darrell Miles wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Chris,
}
} If you already know, or have tried this, then just ignore my post.
} You may be able to do better with the equipment you have.
}
} Depending on the coater(s) you have at your disposal, if you can
} control the current and time of the coater system, you can greatly
} improve the coating for high magnification work by lowering the
} current setting, and running for a longer time. The lower current
} slows the deposition. The deposited film turns out more uniform,
} without forming "clumps". Gold-palladium targets seem to do
} better than straight gold. Adjusting the time will control the
} thickness. This is how we survived when we first started working
} with the higher magnifications in an FEGSEM.
}
} Regards,
} Darrell
}
} "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk} on 03/22/2002 10:34:33 AM
}
} Please respond to c.jeffree-at-ed.ac.uk
}
} To: microscopy-at-sparc5.microscopy.com
} cc:
} Subject: Coating for FEGSEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
}
} having acquired a FEGSEM, I am now only too well aware of the
} shortcomings of traditional sputter coatings - thick, granular, detail-
} smothering gloop obscuring ultrastructure. What do you currently
} advise as the best and most cost-effective method of obtaining
} quality coatings in the 1 to 2 nm range?
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed Mar 27 12:37:08 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 27 Mar 2002 12:29:03 -0600
Subject: RE: QuartzPCI database help requested

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am using WinNT and have managed to crash Quartz PCI a number of times, but
have been lucky in that no Dbase was corrupted. Just in case that should
occur, I sometimes copy the Dbase files to CD-R along with "volume" data
when transfering to removable media. The worst that happens (so far) is
that PCI remembers "deep down inside" it has stored an image, but will not
display the fact to the user. That is... No query will show evidence of
it's presence. However, if you try to resave using the same file name, PCI
will tell you there is already a file by that name in the Dbase and refuse.
(in a list but lost a pointer???) I have to assign a new file name and save
again. All seems well except you can't "move" the lost file to removable
media.

You might try the pulldown menu: Database -} Database Administration -}
Verify Database -} and see if it will point you to the problem - or fix it.

Goody Luck!
Woody White

} ---------.
}
}
} I was in the process of printing the results of a query
} when my PC
} locked.. froze.. crashed, -at-#$%&!!!, etc...... I was forced to
} reboot. The
} nature of my system failure has not been determined: older PC
} run windows
} 95, several applications were open, using a dbase shared on a network
} server, printing to a network printer, in short, probably not an issue
} related to the PCI application. After scan disk worked it's magic I
} attempted to open the database. Unfortunately I encountered
} a problem with
} a flashing PCI "open database error" window: Could not open
} database -
} index is out of date. I was unable to open the database. I would
} appreciate any advice, preferably good, regarding how to
} correct a database
} error of this type.
}
} FYI This is the only database that seems to be
} affected, so it does
} not appear to by an application error. Attempts to open the
} database from
} several PC's produced the same results. PCI Quartz workgroup
} version 5.10
} build 4207
}
}


From daemon Wed Mar 27 14:29:42 2002



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Wed, 27 Mar 2002 15:21:48 -0500
Subject: RE:QuartzPCI database help requested

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Woody,

We operate on a network server that is backed-up nightly so we are protected
for the most part. However, convincing IT that a timely restoration is in
order can occasionally require a Herculean effort.

I have just gotten a call back from my trusty PCI contact, Carl Hordines of
Hitachi. By running the database administration function called "verify"
database, we were able to quickly restore the database. No information was
lost and all appears to be well.


I'm not sure why your database tables drop or lose information but I can
tell you why the database gives you an error when trying to re-save the
image. Quartz does not embed the image into the database, it uses pointers
to the file. If the database reference to an image is "lost" you will not
find it in the database. However, my guess is that if you go through NT
explorer and bullet down to the correct folder, you will find that your
image file, in fact, is present on the hard drive (database volume). Which
explains the "file already exists" error message when you try to re-save the
image with the same name. Try the following steps to get the image into
your database. Using NT explorer (or windows explorer) bullet down to the
image you want to add to the database. If the image is in the correct
folder, right click the image, select "send to", then select PCI quartz.
You will then need to enter the correct database information as prompted.
PCI will then add the file to the dbase and place a pointer to its current
location. It will not attempt to move, or copy the file. If you use the
import function PCI will try to write the file to the appropriate volume,
however, this will in fact be the same location and you will get a write
error. Only use the import function if the file your are importing resides
in a different path than the database volume.

Woody, thanks for the reply, I appreciate your assistance. If you have no
objections I would like to add your name to my list of PCI user contacts.
If I can be of any assistance please feel free to contact me directly.

Thank you,

} John A. Robson
} _____________________________________________________
}
} Boehringer Ingelheim Pharmaceuticals, Inc.
} Research and Development
} 900 Ridgebury Road / P. O. Box 368
} Ridgefield, CT 06877-0368
}
} phone: 203.798.5640
} fax: 203.798.5698
} email: jrobson-at-rdg.boehringer-ingelheim.com
}
}
}
} -----Original Message-----
} From: White, Woody N. [SMTP:nwwhite-at-mcdermott.com]
} Sent: Wednesday, March 27, 2002 1:29 PM
} To: Microscopy-at-sparc5.microscopy.com;
} 'jrobson-at-rdg.boehringer-ingelheim.com'
} Subject: RE:QuartzPCI database help requested
}
} I am using WinNT and have managed to crash Quartz PCI a number of times,
} but
} have been lucky in that no Dbase was corrupted. Just in case that should
} occur, I sometimes copy the Dbase files to CD-R along with "volume" data
} when transfering to removable media. The worst that happens (so far) is
} that PCI remembers "deep down inside" it has stored an image, but will not
} display the fact to the user. That is... No query will show evidence of
} it's presence. However, if you try to resave using the same file name,
} PCI
} will tell you there is already a file by that name in the Dbase and
} refuse.
} (in a list but lost a pointer???) I have to assign a new file name and
} save
} again. All seems well except you can't "move" the lost file to removable
} media.
}
} You might try the pulldown menu: Database -} Database Administration -}
} Verify Database -} and see if it will point you to the problem - or fix
} it.
}
} Goody Luck!
} Woody White
}
} } ---------.
} }
} }
} } I was in the process of printing the results of a query
} } when my PC
} } locked.. froze.. crashed, -at-#$%&!!!, etc...... I was forced to
} } reboot. The
} } nature of my system failure has not been determined: older PC
} } run windows
} } 95, several applications were open, using a dbase shared on a network
} } server, printing to a network printer, in short, probably not an issue
} } related to the PCI application. After scan disk worked it's magic I
} } attempted to open the database. Unfortunately I encountered
} } a problem with
} } a flashing PCI "open database error" window: Could not open
} } database -
} } index is out of date. I was unable to open the database. I would
} } appreciate any advice, preferably good, regarding how to
} } correct a database
} } error of this type.
} }
} } FYI This is the only database that seems to be
} } affected, so it does
} } not appear to by an application error. Attempts to open the
} } database from
} } several PC's produced the same results. PCI Quartz workgroup
} } version 5.10
} } build 4207
} }
} }



From daemon Wed Mar 27 14:38:05 2002



From: Matt Olszta :      molsz-at-mse.ufl.edu
Date: Wed, 27 Mar 2002 15:28:27 -0500
Subject: Diamond Knife for Mineralized Tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am in the market to purchase a diamond knife for microtoming mineralized
collagen samples. Has anyone done microtoming on mineralized tissues, and
if so, what type of diamond knife (angle and company) did you use? Any help
is appreciated.

Regards,
Matt Olszta
University of Florida
Material Science and Engineering



From daemon Wed Mar 27 15:38:38 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 27 Mar 2002 16:31:01 -0500
Subject: urgent need page numbers and volume name from M&M 2001 meeting pr

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have two abstracts in the Microscopy and Microanalysis 2001 proceedings that I urgently need the page numbers. I also need the volume number for the proceedings. Unfortunately, they are at home and I need them right now and nobody else here has a copy. Would some kind soul email me the information and make it public on the server so that there isn't duplication. It would be greatly appreciated. Thanks.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)




From daemon Wed Mar 27 15:47:00 2002



From: Larry Allard :      L2A-at-ornl.gov
Date: Wed, 27 Mar 2002 16:41:51 -0500
Subject: Re: urgent need page numbers and volume name from M&M 2001 meeting pr

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott:

here they are:

1. XPS evaluation .... P892

2. Prethinning.... P952

Microscopy and Microanalysis V.7, Suppl.2 Proceedings




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Wed Mar 27 15:57:03 2002



From: James Long :      james_long-at-tedpella.com
Date: Wed, 27 Mar 2002 15:49:20 -0600
Subject: Coating for FEGSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chris,

As you can tell from the posts so far, there are many different ways to
approach high resolution coating. Something to consider is that probably no
one method is best for all samples, so before you spend any money, I would
encourage you to try several types of coating on your samples, and see which
one gives you the best results.

Please contact me offline for more information and sample specific
discussion.

James

Disclaimer: Ted Pella, Inc. sells Cressington sample preparation equipment
including basic coaters, FESEM Chromium coaters, and vacuum evaporators.



James Long
Materials Science Specialist
Ted Pella, Inc.
512-657-0898
james_long-at-tedpella.com {mailto:james_long-at-tedpella.com}
www.tedpella.com {http://www.tedpella.com}





-----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
Sent: Friday, March 22, 2002 9:35 AM
To: microscopy-at-sparc5.microscopy.com


Dear All

having acquired a FEGSEM, I am now only too well aware of the
shortcomings of traditional sputter coatings - thick, granular, detail-
smothering gloop obscuring ultrastructure. What do you currently
advise as the best and most cost-effective method of obtaining
quality coatings in the 1 to 2 nm range?

Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================



From daemon Wed Mar 27 18:36:01 2002



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 27 Mar 2002 18:28:02 -0600
Subject: Re: Coating for FEGSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'll second the vote for the use of Ir for FESEM imaging. We also have the
SouthBay
Tech IBS/e with an Ir target - it works wonderful for us. We also have it
set up for Carbon, Pt, and Pd and Ta, but we only use these other metals if
we need to avoid an interference by EDS for detection of some low level
component. We The Ir works great for us with daily use in the mag range of
50KX to 250KX on a variety of materials. Although I have not tried to do
so, I still have not imaged the strucure of the Ir on sputtered specimens by
FESEM.

In the past we used Au and Au/Pd in an OLD Denton DESK sputter coater, and
an OLD Ladd Vacuum Evaporator, and we always found that Au/Pd was far
superior to Au. But, as soon as we got the FESEM, we were shocked by the
structure that we were adding with those "coatings" of Au/Pd.


Brad Huggins
BP Chemicals
Naperville, IL


-----Original Message-----
} From: Becky Holdford [mailto:r-holdford-at-ti.com]
Sent: Wednesday, March 27, 2002 12:02 PM
To: Microscopy-at-sparc5.microscopy.com


Au-Pd is better than plain Au for FESEM, in my experience, but Iridium is
better than either one.
We have 2 Ir sputterers, one is an magnetron planar type (Emitech K-575) w/
an Ir target and
the other is an ion-beam sputter coater (an ancient version of the SouthBay
Tech IBS/e) with an
Ir target. The lab favorite seems to be ion beam coater as the techs think
it gives a "better"
coating for working at or above 100KX. We are a semiconductor house, so we
routinely work at
mags of 100KX to 300KX. This is not quantitative date, just people's
preferences.
I don't like chromium coatings due to the oxidation problem.
DISCLAIMER: all opinions are strictly my own, and I have no interest,
financial or otherwise,
in either Emitech or SouthBay Technologies. I just buy their stuff and use
it.

Darrell Miles wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Chris,
}
} If you already know, or have tried this, then just ignore my post.
} You may be able to do better with the equipment you have.
}
} Depending on the coater(s) you have at your disposal, if you can
} control the current and time of the coater system, you can greatly
} improve the coating for high magnification work by lowering the
} current setting, and running for a longer time. The lower current
} slows the deposition. The deposited film turns out more uniform,
} without forming "clumps". Gold-palladium targets seem to do
} better than straight gold. Adjusting the time will control the
} thickness. This is how we survived when we first started working
} with the higher magnifications in an FEGSEM.
}
} Regards,
} Darrell
}
} "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk} on 03/22/2002 10:34:33 AM
}
} Please respond to c.jeffree-at-ed.ac.uk
}
} To: microscopy-at-sparc5.microscopy.com
} cc:
} Subject: Coating for FEGSEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
}
} having acquired a FEGSEM, I am now only too well aware of the
} shortcomings of traditional sputter coatings - thick, granular, detail-
} smothering gloop obscuring ultrastructure. What do you currently
} advise as the best and most cost-effective method of obtaining
} quality coatings in the 1 to 2 nm range?
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed Mar 27 18:53:43 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 27 Mar 2002 16:53:21 -0800
Subject: Re: converting digital camera to microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This issue is currently being discussed on the usenet at:

sci.techniques.microscopy

Try connecting there are multiple postings
under the header

Coolpix ?'s

Some posts are very informative.

gary g.


At 09:21 AM 3/27/2002, you wrote:

} Hi All,
} I have a question on behalf of colleague of mine who is considering his
} options for adding a digital camera to his Zeiss Axiovert and stereo
} microscopes. He has heard/read of adapting a Nikon Coolpix for this use,
} using a special adapter. Is there anyone out there who can offer advice as
} to whether this works well and how it works?
} Thanks in advance for your advice,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Department of Plant Pathology
} 351 Bessey Hall
} Iowa State University
} Ames, IA 50011
}
} 515-294-8854
} kalen-at-iastate.edu
} www.baumlab.org
}
}



From daemon Thu Mar 28 00:46:39 2002



From: Peter Jordan :      emsi-at-pe.net
Date: Wed, 27 Mar 2002 22:27:15 -0800
Subject: Wanted: Zeiss 10C TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:
Looking for a good used Zeiss 10C TEM. Please let me know if you have one
available. We are in the Los Angeles area.
Tanks, Peter Jordan




From daemon Thu Mar 28 07:10:34 2002



From: RangeTS-at-aol.com
Date: Thu, 28 Mar 2002 07:57:54 EST
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please unsubscribe.
Thank You.


From daemon Thu Mar 28 07:20:55 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 28 Mar 2002 07:10:03 -0600
Subject: RE: QuartzPCI _Comments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John,

Thanks for the hint. Will give the "send to" a try. The only network I am
allowed to have is the peer/peer between the SEM PC and my EDS PC - side by
side. I store data to the EDS PC since it has more power and a CD-R (than
the SEM PC). Given that, I am very aware of where the actual image data is
stored. If I don't burn CDs, it would never get backed up. You are correct
about the "lost" images... They are certainly there in the proper file.
Apparently PCI lost the pointer in the process of crashing.

Here is another one that is not a big problem, but I am curious... Every
now and again I get a corrupt thumbnail image. Typically one of two modes.
Either a partial/corrupted T/N image or a totally black field for a T/N.
Ever seen this?

YES! Please add me to any communications re Q_PCI!

Thanks,
Woody White
McDermott Technology Inc
McDermott site: http://www.mtiresearch.com/
Personal site: http://woody.white.home.att.net


From daemon Thu Mar 28 07:34:06 2002



From: Rich Fiore :      rafiore-at-unity.ncsu.edu
Date: Thu, 28 Mar 2002 08:28:28 -0500
Subject: SI(Li) detector response time

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear collegues,

Does anyone know of a reference of Si(Li) detector response time? I need
this for a nuclear application.

Thanks,

Rich

Rich Fiore
NC State University
Analytical Instrumentation Facility
1010 Main Campus Drive
318 Engineering Graduate Research Ctr., Box 7531
Raleigh, NC 27695
Tel: 919-515-2348
Fax: 919-515-6965
Email: rich_fiore-at-ncsu.edu



From daemon Thu Mar 28 09:10:51 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Thu, 28 Mar 2002 08:47:39 -0600
Subject: Suggestions on serial sections of tissue in plastic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listies,

I'm attempting to cut serial sections of pin feathers in plastic
(Embed12/Spurrs) at 1-2 µ thick for light microscope evaluation.

The pin feathers are approx 1.5 cm long so I'll have a lot of cutting to
do. I'm going to use glass knives.

Can anyone recommend an efficient procedure to pick up the sections and
place them on glass slides. I've used a loop in the past with good results,
but the limitation of amount of sections I can pick up in a loop could make
for a very long project time.

Should I use a diluted acetone solution, say 10-20% in the boat?

I'd also like to use a H&E stain. Do I need to make any modifications for
plastic?

As always,
Thanks,
Tim Quinn
University of Kansas
Program Assistant
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu


From daemon Thu Mar 28 09:19:57 2002



From: Scott D. Davilla :      davilla-at-4pi.com (by way of MicroscopyListserver)
Date: Thu, 28 Mar 2002 09:11:51 -0600
Subject: Re: SI(Li) detector response time

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Does anyone know of a reference of Si(Li) detector response time? I need
} this for a nuclear application.

Need a better definition for response time, do you want the Si(Li)
(physical) or do you want the electrical (when the signal comes out the
preamp) or after the filter amp has processed or the total time to when the
signal is converted and stored in memory?

Scott

-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com


From daemon Thu Mar 28 09:21:29 2002



From: ldm microscopy :      ldm2-at-risc4.numis.nwu.edu
Date: Thu, 28 Mar 2002 09:20:29 -0600 (CST)
Subject: Microscopy visualizations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am going to be teaching an undergraduate (senior)
TEM (with perhaps a little SEM) class next quarter.
I had a quick look this morning on the web, and did
not find much in the way of visualizations (e.g. Java).
If you know of any, please let me know (not to the
listserver); I will summarize what I find.

-----------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
-----------------------------------------------




From daemon Thu Mar 28 09:46:11 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 28 Mar 2002 09:38:15 -0600
Subject: RE: SI(Li) detector response time

Contents Retrieved from Microscopy Listserver Archives
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Hi Rich,

Don't have the data, but a question and comment...

Are you looking for the response of the crystal alone or that of the crystal
and first FET preamp?

If the latter, perhaps you can look at the output using a fast oscilloscope
and observe the rise/fall times for a given x-ray input. If you do this, be
sure the load resistance and reactance, when hooked to the o'scope, is the
same as the end application.

Woody White
------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear collegues,
}
} Does anyone know of a reference of Si(Li) detector response
} time? I need
} this for a nuclear application.
}
} Thanks,
}
} Rich
}
} Rich Fiore
} NC State University
} Analytical Instrumentation Facility
} 1010 Main Campus Drive
} 318 Engineering Graduate Research Ctr., Box 7531
} Raleigh, NC 27695
} Tel: 919-515-2348
} Fax: 919-515-6965
} Email: rich_fiore-at-ncsu.edu
}
}


From daemon Thu Mar 28 10:15:35 2002



From: Robert Schoonhoven :      rschoon-at-email.unc.edu
Date: Thu, 28 Mar 2002 11:16:18 -0500
Subject: SEM Methods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Afternoon.

I, Denise Kukich, am one of Dr. Schoonhoven's graduate students, and am
involved with a project in one of my classes that requires me to
research innovative methods in SEM. Could you please send SEM
information or web sites to me at dmbrown-at-email.unc.edu. Thank you so
much for your time.

Sincerely,
Denise Kukich
Ph.D. Candidate/Research Assistant
Dept. of Environmental Sciences and Engineering
UNC Chapel Hill


--
best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7431
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first.
Mark Twain [Samuel Langhornne Clemens] (1835-1910)


From daemon Thu Mar 28 10:25:39 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 28 Mar 2002 08:25:25 -0800
Subject: RE: converting digital camera to microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've received several questions about this.

It is usenet, not the web. An ISP has newsreader
service typically via news.isp.com or news.isp.net
where isp is the name of the ISP.

Since you are .gov, you may not have access
to this if you are behind a firewall that prohibits
usenet. Even though you most likely have an external
ISP for main Internet access.

For those who can't access the usenet, I've copied
the message bodies into this posting:

} I am contemplating the purchase of a Coolpix camera for macrophotography
} and microphotography. I have read many messages on this group saying
} how good the 990 and 995 work for these types of applications but I was
} wondering......
}
} If you had a choice which would you prefer the 990 or 995 for
} photomicroscopy?
}
} There are a number of third party couplers for the coolpix to
} microscope. Which "coupler" works the best? Which should I avoid?
}
} Most messages talk about how well the Coolpix works as a microscope
} camera. Are there any problems with it? Nothing can be perfect! Can
} it??
}
} Thanks
} dale

} Hello,
}
} I have been working extensively with the Coolpix 990. In general the
} camera itself works well. As far as I know the two models differ in
} that the 995 uses the newer and thicker compact flash memory
} cards/mini disk memory and the flash unit pops up and away from the
} camera body to prevent red eyd in portraits. There may be other minor
} differences as well but for use at the microscope they are
} functionally the same. The Coolpix is able to close fcus without
} other lenes and is capable of some macro work with out additional
} optics which are also available.
}
} The main problems with consumer cameras are 1) they use an
} anti-aliasing routine to "soften" the image to prevent some odd pixel
} effects and 2). the CCD chips are not physically cooled to limit the
} thermal noise which in turn reduces the dynamic range of the images.
} The design of the camera is not easily altered to circumvent either
} of these issues. The best you can do is to use photo editing software
} to massage and sharpen the image.
}
} Paranthetically, here is some information on anti-aliasing filters.
} http://www.kodak.com/global/en/professional/products/cameras/dcsTech/antiAliasingFilter/antiAliasing.jhtml
}
} There is no clear-cut best coupler.
}
} High end photographic systems designed for use with a microscope use a
} specially designed projection lens (in place of an eyepiece) to cast
} the image on the film. Camera backs are used without other
} photographic lenses.
}
} The 990 and 995 do not have removable lenses which means an eyepiece
} of one sort or another is required to relay the image to the camera
} lens. ( In this circumstance, the camera lens is set to focus at
} infinity and the aperture is set at its widest. The image is focused
} with the microscope controls and should be parfocal with the image at
} the eyepieces. The only problem will be with your own eyesight. The
} camera will not focus at the same point as your eyes so you will need
} to use your glasses to focus unless you develope a standard correction
} to compensate for the difference.)
}
} Herein lies the rub. Each of the microscope manufacturers builds into
} their eyepieces compensation for residual uncorrected abberitions in
} their objectives. These corrections are unique for the manufacturer
} and for the human eye which is the normal primary detector.
}
} There are adapters that are designed to connect the Coolpix camera to
} whatever eyepiece is designed for the microscope. Theorectically this
} is the best solution but in practice these may or may not work
} depending on the eyepiece design. My Zeiss widefield high eyepoint
} eyepieces vignette severly when using this type of adapter. This type
} adapter has not provided adequate results for me.
}
} So I must use "another" eyepiece. I have used a Leitz Periplan
} eyepiece that happily screws onto the Coolpix and found that this
} pretty much works but not to perfection. I have also used another
} eyepiece adapter designed by Optem specificaly for use with the
} Coolpix. Here again the results are different from the Leitz
} eyepiece but the conclusion is the same, pretty good but not perfect.
}
} The point is that the digital images can be striking and quite
} appealing IF you have never seen the same image at the microscope.
} The best images I have ever captured are a 6 or 7 on a scale of
} 1(worst) to 10 (best) when compared to the vue at the scope. This is
} frustrating and I am still searching for an answer.
}
} Therefore, I have made a compromise because the costs of a camera that
} comes closer to perfection is going to cost between 3 and 8 times as
} much as the Coolpix..

} Great input. Thanks. Here are my two cents added:
}
} } Herein lies the rub. Each of the microscope manufacturers builds into
} } their eyepieces compensation for residual uncorrected abberitions in
} } their objectives. These corrections are unique for the manufacturer
} } and for the human eye which is the normal primary detector.
} }
}
} At least from what I was told, Nikon's new CFI60 corrects aberration
} independently in eye-piece and objective. (How much of this is true, I do
} not know.)
}
} According to Nikon's CFI60 optics brochure (code no. 2CEMUN5) page 3, they
} claim "...both axial and lateral chromatic aberration have been corrected
} independently in the objective and the tube lens. CFI60 objectives are
} designed to produce flat images without the aid of other components,
} allowing their use in applications other than microscopy". - I can only
} assume that other manufactures will follow this approach if possible.
}
} When using Nikon's MDC relay lens (sold for Coolpix 995) as an eye-piece,
} I get a very nice 18mm F.O.V. image when looking through it. But when using
} the Coolpix, the recorded image is much worse. (Thanks for your hint
} regarding anti-aliasing routine.)
}
} } The point is that the digital images can be striking and quite
} } appealing IF you have never seen the same image at the microscope.
} } The best images I have ever captured are a 6 or 7 on a scale of
} } 1(worst) to 10 (best) when compared to the vue at the scope. This is
} } frustrating and I am still searching for an answer.
} }
}
} I assume this is a logarithmic scale. With my scope, I would only give
} it a five under the best of circumstances. I can recognize the image but
} when knowing the "original", it is pretty bad.
}
} I tried Coolpix 995 with Nikon MDC relay lens and SONY DSC-S70 with an MDC
} relay lens (using a simple home-made adapter). All are mounted directly to
} an ISO 38mm phototube to ensure a mechanically stable setup. (I used manual
} setup
} with various different settings as recommended in this news-group earlier. N
} othing
} made me feel good about these images.)
}
} My next candidate will be PixeLink and Pixera. Most likely, I will soon
} spent more money on the digital setup than I spent for my scope.
}
} (BTW, the coolpix and dsc-s70 are two very nice digital cameras, which have
} not been optimized for photomicrography. I do not recommend them for
} photomicrography if you do not already have them.)
}
} GTO

} I believe that the threads on the 995 are plastic and metal on the 990. If
} that's the case if you are changing the camera much I would prefer the metal
} threads.

} First, the comments in the Nikon literature about their relay lens
} applies to Nikon microscopes and objectives.. Each microscope maker
} manages to do the job differently. If you have the right series of
} Nikon scope bingo!!
}
} Second, all the consumer cameras have the anti-aliasing and dynamic
} range problems. Even for regular photography the consensus is that
} the images require extensive massaging with a photo editor for serious
} work. There is lots of discussion on the web.
}
} The problems with software processing are amplification of noise, loss
} of fine textures and halos around the sharp edges.
}
} Anyone who aspires to excellent digital images today is also required
} to have a pretty deep understanding of photo editing and image
} enhancement routines. The Unsharpen Mask routine is somewhat
} successful in undoing the anti-aliasing. There are special 3rd party
} plugins for the Adobe photo editor dealing with this issue in addition
} to the standard routines. You need to experiment with.the variable
} parameters for these routines.
}
} As a matter of fact the forefront of light microscope today is
} real-time electronic image processing to remove noise, stray light and
} unwanted background details while improving contrast and amplifying
} details. VEC (Video Enhanced Microscopy) deals with low contrast,
} flat images and unwanted backgrounds. VIM ( Video Imaging
} Microscopy) deals with extreme low levels of light. Both are hot
} concepts with big time expensive electronics to add to the normal
} microscope optics.
}
} Also the area of computer deconvolution of digital images taken with
} scientific instruments is making big strides. AutoQuant produces
} AutoDeblur software with which I have been sucessfully experimenting.
} They offer a full featured demo package with a limited 30 day access
} at www.autoquant.com . The package includes manuals and tutorial
} images. I may post some more information as a sepeerate topic in this
} NG later.
}
} Third, Gary Gaugler, who is a regular contributor to this NG and very
} discerning about the quality of his images highly recommends the
} Pixera. He has become a distributor. See his site at
} http://photoweb.net/
}
} Remember, however, the Pixera cameras recommended for top end
} microscopy are in the $6K to $8K range. This is a far cry from the
} more practical Coolpix 990/995. which purchased today is less than
} $1.5K for camea and all the other necessary accessories, ie.adapeter,
} remote control, power adapter, bigger memory cards, etc..
}
} With all this sais about the shortcomings of the Coolpix, I wanted to
} make available some images that I have produced with the Coolpix,
} eyepiece adapters and Corel Photopaint software for folks to make
} their own determinations about the utility of this system.
} Unfortunately, FTP access to my web location is "down." For now see
} the addendum below for images that are already accessable from the
} web. When I have FTP access, I will post links to more recent images
} which reflect improved skills on my part. Check back in about 4-5
} days for the others
}
} Lastly, for those of you wondering where all my informations comes
} from, here are two recently published books I have studied.
}
} "Photography with a Microscope" by Fred Rost and Ron Oldfield
}
} "Light Microscopy in Biology" (A Practical Approach) Edited by A. J.
} Lacey
}
}
} Addendum
}
} Lnks to digital photomicrographs produced with a Coolpix 990 mounted
} on my Zeiss microscpe and processed with Corel Photopaint 10 software.
} Any comments welcome. Email nghy-at-comcast.net.
}
} Demodex mite - Transmitted light DIC - 40X Neofluar objective
} http://www.microscopy-uk.org.uk/mag/artmar02/amdemodex.html
}
} 8 Diatom test slide
} http://www.microscopy-uk.org.uk/mag/artjan02/amdiatoms.html
}
} Intel 486 CPU - Epi-DIC Survey images at 50X and 100X, detail images
} at 500X.
} http://mywebpages.comcast.net/nghy/chips/

} If there is some interest, I can put the "best" Coolpix images I got on my
} web-page. Mainly histology stuff but maybe informative.
}
} Deconvolution methods are ok. But why spend money for it or play with
} time-stamped SW packages. Try ImageJ from http://rsb.info.nih.gov/ij/, it
} can already do a lot. Personally, I prefer to spend most of the money on HW
} rather than on SW.
}
} GTO

} "gto" {gregor_o-at-NOSPAMyahoo.com} wrote in message
} news:nrao8.6408$E64.1228308602-at-newssvr15.news.prodigy.com...
} } If there is some interest, I can put the "best" Coolpix images I got on my
} } web-page. Mainly histology stuff but maybe informative.
} }
} } Deconvolution methods are ok. But why spend money for it or play with
} } time-stamped SW packages. Try ImageJ from http://rsb.info.nih.gov/ij/, it
} } can already do a lot. Personally, I prefer to spend most of the money on
} HW
} } rather than on SW.
} }
} } GTO
} The nice thing about imageJ is it always expanding with user written
} plugins. As far as image processing goes it is far more powerful most
} commercial packages. If it doesn't do to suit you, you can write your own
} plugin to suite your self:) Generally you can find one that is close an
} modify it or con the fellow that wrote it into modifying it.
} --
} Gordon

} Aquinto in Germany does a package for image archiving / analysis with a
} special starter package for the COllpix. It includes an adapter that screws
} to a 0.63 C mount adapter.
}
} You can contact them at www.aquinto.de
}
} I missed the earloer portion of the thread, but thesystem can use a range of
} camera systems also.These range from the hi Res Nikon 1200's, and lower res
} Valer, and even analogue systems.
}
} If you in the UK. You can contact me direct. If not try them for your
} nearest distributer. The company I work for supplies the packages, and they
} are very good IMHO.
}
} Hope thats of some help.
}
} Kevin





At 07:20 AM 3/28/2002, you wrote:
} What is the complete extension?? I have used org, net, & com, but they do
} not work. Thanks.
}
} -----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: Wednesday, March 27, 2002 6:53 PM
} To: Kristen Lennon
} Cc: MSA listserver
} Subject: Re: converting digital camera to microscope
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Mar 28 12:57:12 2002



From: James Talbot :      james-at-ktgeo.com
Date: Thu, 28 Mar 2002 12:47:40 -0800
Subject: Re: Filters fort dust particle collection - ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Coetzee-

There is a company that makes silver metal membrane filters that I believe are
used for dust collection puroposes. The filters are not cheap but they are
durable. The company is Sterlitech Corporation and their contact info follows:

Mark J. Spatz, President
Sterlitech Corporation
22027 70th Avenue S
Cumberland Industrial Center
Kent, WA 98032-1911 USA
Tel: 253-437-0844 or 877-544-4420
Fax: 253-437-0845
Email: mspatz-at-sterlitech.com
Web: http://www.sterlitech.com

Disclaimer: I have no interest whatsoever in this company.

Sincerely,
James Talbot

K/T GeoServices, Inc.
Bulk and Clay Mineralogy by X-ray Diffraction
(940) 597-9076
web site: http://www.ktgeo.com/



"Coetzee, Mr S. H Physics Science" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
}
} There was a discussion related to filters. Unfortunately I did not follow
} it. We have a student interested to do dust collection at remote areas here
} in Botswana and is looking for a filter with a smooth surface that is
} durable. The filter must also be beam-stable since we want to do EDS
} particle analysis afterward. Some filters are treated with sulphur to
} reduce static charges. We will be interested in sulphur particles as well.
}
} Please help
}
} Mr S. H. Coetzee
} Electron Microscope Unit
} University of Botswana
} Private Bag 0022
} Gabarone
} Botswana
} { {...OLE_Obj...} }
}
} Phone : +267 355 2426
} Mobile: +267 718 96 729
} Fax : +267 585 097
} e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}



From daemon Thu Mar 28 13:52:45 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 28 Mar 2002 13:43:48 -0600
Subject: RE: SEM Methods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As with many similar requests, your question is rather broad, vague, and
unlikely to elicit many meaningful replies. I would suggest that first you
do a search using, for example,
( http://www.google.com )
and then ask more specific questions.

Suggested keywords: SEM, scanning electron microscope, eds, energy disperive
spectroscopy, environmental, bsed, ebic, variable pressure, wds, wavelength
dispersive spectroscopy, secondary electron, se, bse, backscattered
electron, x-ray, image, sputter coating, evaporative coating, JEOL, Hitachi,
LEO, FEI, (excuse me for leaving out many manufacturers!) etc....

Use keywords alone or in various combinations. That should keep you rather
busy for a while digesting information.

Woody White

} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Good Afternoon.
}
} I, Denise Kukich, am one of Dr. Schoonhoven's graduate
} students, and am
} involved with a project in one of my classes that requires me to
} research innovative methods in SEM. Could you please send SEM
} information or web sites to me at dmbrown-at-email.unc.edu. Thank you so
} much for your time.
}
} Sincerely,
} Denise Kukich
} Ph.D. Candidate/Research Assistant
} Dept. of Environmental Sciences and Engineering
} UNC Chapel Hill
}
}
} --
} best regards,
} Bob
} Robert Schoonhoven
} Laboratory of Molecular Carcinogenesis and Mutagenesis
} Dept. of Environmental Sciences and Engineering
} University of North Carolina
} CB#7431
} Chapel Hill, NC 27599
} Phone
} office 919-966-6343
} Lab 919-966-6140
} Fax 919-966-6123
}
} Don't go around saying the world owes you a living; the world owes you
} nothing; it was here first.
} Mark Twain [Samuel Langhornne Clemens] (1835-1910)
}


From daemon Thu Mar 28 13:55:20 2002



From: mpfauth-at-teleport.com :      mpfauth-at-teleport.com
Date: Thu, 28 Mar 2002 14:49:55 -0500
Subject: RE: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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--------------------------------------------------------------------
mail2web - Check your email from the web at
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From daemon Fri Mar 29 00:41:01 2002



From: Young W. Kim :      ywkim-at-gong.snu.ac.kr
Date: Fri, 29 Mar 2002 15:34:42 +0900
Subject: Signals from a SEM monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
Our CRT in XL20 SEM Polaroid died last week.
We're trying to replace our Polaroid system by hooking up the PC data acquisition board.
What we're thinking of is to get the signals of vertical and horizontal scan in the Polaroid CRT system, plus amplified analog signal of detector, then feed those 3 signals to PC data acquisition board. If the programming is too much, just collect the secondary electron signal with x-y position of the monitor. Those number matrix can be processed in the Digitalmicrograph.
Has anyone tried this setup? Or is there any commercial hardware/software to replace the Polaroid with PC data acquisition?

Young W. Kim, Ph.D.
Research Professor
School of Materials Science and Engineering
Seoul National University
Kwanak-ku Shinlim-dong San 56-1
Seoul, Republic of Korea 151-744

Tel) + 82-2-880-7977
E-mail) ywkim-at-gong.snu.ac.kr


From daemon Fri Mar 29 08:40:52 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 29 Mar 2002 09:31:00 -0500
Subject: Signals from a SEM monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Young;

We had to do that on a Hitachi S570 [an old SEM] when we interfaced an EDAX
DX4 EDX system for beam control used in elemental mapping and for image
acquisition. On these old SEMs, there is no direct interface that is
brought out to a port from the horizontal and vertical scan generators or
the secondary electron detector which will be the brightness signal. You
will necessarily have to go cut into those points in the video board for
those signals. It would be best to have someone with analog circuit
experience do this since you want to make sure the analog levels are
appropriate and electrically isolated from the acquisition system. And, by
all means, document what was done in the event of failure or mtc.

It's not fun but it can be done.

Regards,
Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Young W. Kim [mailto:ywkim-at-gong.snu.ac.kr]
Sent: Friday, March 29, 2002 1:35 AM
To: Microscopy-at-sparc5.microscopy.com


Listers,
Our CRT in XL20 SEM Polaroid died last week.
We're trying to replace our Polaroid system by hooking up the PC data
acquisition board.
What we're thinking of is to get the signals of vertical and horizontal
scan in the Polaroid CRT system, plus amplified analog signal of detector,
then feed those 3 signals to PC data acquisition board. If the programming
is too much, just collect the secondary electron signal with x-y position
of the monitor. Those number matrix can be processed in the
Digitalmicrograph.
Has anyone tried this setup? Or is there any commercial hardware/software
to replace the Polaroid with PC data acquisition?

Young W. Kim, Ph.D.
Research Professor
School of Materials Science and Engineering
Seoul National University
Kwanak-ku Shinlim-dong San 56-1
Seoul, Republic of Korea 151-744

Tel) + 82-2-880-7977
E-mail) ywkim-at-gong.snu.ac.kr


From daemon Fri Mar 29 08:40:55 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Fri, 29 Mar 2002 11:05:26 -0330
Subject: newgroup access (was RE: converting digital camera to microscope)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary writes ...

} I've received several questions about this.
}
} It is usenet, not the web. An ISP has newsreader
} service typically via news.isp.com or news.isp.net
} where isp is the name of the ISP.
}
} Since you are .gov, you may not have access
} to this if you are behind a firewall that prohibits
} usenet. Even though you most likely have an external
} ISP for main Internet access.
}
} For those who can't access the usenet, ...

Web browser access is provided by Google.groups ... e.g.,
http://groups.google.com/groups?hl=en&lr=lang_en&newwindow=1&group=sci.techn
iques.microscopy

or, for example ...
http://groups.google.com/groups?hl=en&safe=off&group=rec.crafts.brewing

or, advanced searching
http://groups.google.com/advanced_group_search

Leaving a new post or reply does require that you register ...
http://groups.google.com/googlegroups/posting_faq.html

The upside is ALL newsgroups are represented, and all messages are
archived here (... every dumb question has already been answered ...
possibly?). On the downside ... posts take several hours to be posted, and
I would take the suggestion for using an e-mail alias seriously ... see
http://groups.google.com/googlegroups/posting_faq.html#email

genuinely ... michael shaffer :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From daemon Fri Mar 29 08:43:18 2002



From: tuttle-at-cox.net
Date: Fri, 29 Mar 2002 07:42:32 -0700
Subject: RE: converting digital camera to microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The XL 20 has a video printer out put which you could use to print images.
We have a laser printer connected to our XL 20 computer and get useable
images at 600 dpi on regular paper. A high-end ink jet printer and photo
quality paper should give very nice results. Do you have the soft ware
upgrade that includes the "Save and Print" option?

Ron L
----- Original Message -----
} From: "Young W. Kim" {ywkim-at-gong.snu.ac.kr}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, March 29, 2002 1:34 AM


Everyone with access to the web can read and post (not binaries
though) to usenet through http://groups.google.com .

So, for example you want to see every post that mentions coolpix in
sci.techniques.microscopy you use :

groups.google.com/groups?as_q=coolpix&as_ugroup=sci.techniques.microsc
opy (url may need to be cut and pasted together).

Regards, Dave Harrison

On 28 Mar 2002 at 8:25, Gary Gaugler wrote:

}
} I've received several questions about this.
}
} It is usenet, not the web. An ISP has newsreader
} service typically via news.isp.com or news.isp.net
} where isp is the name of the ISP.
}
} Since you are .gov, you may not have access
} to this if you are behind a firewall that prohibits
} usenet. Even though you most likely have an external
} ISP for main Internet access.
}
} For those who can't access the usenet, I've copied
} the message bodies into this posting:



From daemon Fri Mar 29 09:29:36 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 29 Mar 2002 08:28:01 -0700
Subject: Signals from a SEM monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What you are describing is a fairly standard digital image acquisition
system. There are many vendors who sell these systems (we are one of them).
The system you are describing is a passive system, which collects the
signals as they are coming from the SEM and converts them into an image.
For the XL20, which is a relatively new instrument, it should almost be
"plug and play".

Contact me offline if you need more information. We do have a sales partner
in Korea.

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Young W. Kim [mailto:ywkim-at-gong.snu.ac.kr]
Sent: Thursday, March 28, 2002 11:35 PM
To: Microscopy-at-sparc5.microscopy.com


Listers,
Our CRT in XL20 SEM Polaroid died last week.
We're trying to replace our Polaroid system by hooking up the PC data
acquisition board.
What we're thinking of is to get the signals of vertical and horizontal
scan in the Polaroid CRT system, plus amplified analog signal of detector,
then feed those 3 signals to PC data acquisition board. If the programming
is too much, just collect the secondary electron signal with x-y position
of the monitor. Those number matrix can be processed in the
Digitalmicrograph.
Has anyone tried this setup? Or is there any commercial hardware/software
to replace the Polaroid with PC data acquisition?

Young W. Kim, Ph.D.
Research Professor
School of Materials Science and Engineering
Seoul National University
Kwanak-ku Shinlim-dong San 56-1
Seoul, Republic of Korea 151-744

Tel) + 82-2-880-7977
E-mail) ywkim-at-gong.snu.ac.kr


From daemon Fri Mar 29 12:06:37 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 29 Mar 2002 10:05:27 -0800
Subject: Re: newgroup access (was RE: converting digital camera to

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can also use

www.dejanews.com

to look up all postings in any group.

gary

At 06:35 AM 3/29/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 29 12:06:38 2002



From: Jim Haley :      haley-at-mvia.com
Date: Fri, 29 Mar 2002 12:59:08 -0500
Subject: Re: converting digital camera to microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kristen,

We do offer adapters for the Coolpix camera to mount to just about any
scope. We offer a 30 day money back guarantee if you're not satisfied
with the adapter. The coupler is specifically designed for use with the
Coolpix camera to ensure you have the full zoom range of the camera with
no vignetting or chromatic aberrations present with many of the other
adapters out there.

If you're not committed to the Coolpix camera at this point, we also
have some really nice firewire, live display cameras that put the images
directly into a computer at close to video rate on our website (see
below for link).

Please feel free to email or call if you have any questions or would
like additional information.

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
2901 Duckettown Road
Laurel, MD 20708
voice: (301) 809-6292
fax: (301) 805-2819
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************
}
} Kristen Lennon wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hi All,
} } I have a question on behalf of colleague of mine who is considering his
} } options for adding a digital camera to his Zeiss Axiovert and stereo
} } microscopes. He has heard/read of adapting a Nikon Coolpix for this use,
} } using a special adapter. Is there anyone out there who can offer advice as
} } to whether this works well and how it works?
} } Thanks in advance for your advice,
} } Kristen
} }
} } Kristen A. Lennon, Ph.D.
} } Department of Plant Pathology
} } 351 Bessey Hall
} } Iowa State University
} } Ames, IA 50011
} }
} } 515-294-8854
} } kalen-at-iastate.edu
} } www.baumlab.org
}
} --

--



From daemon Fri Mar 29 12:10:13 2002



From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Fri, 29 Mar 2002 10:08:19 -0800
Subject: Re: Suggestions on serial sections of tissue in plastic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When I had a project requiring many serial sections I had our machine shop
make a special knife boat. The trough was large enough to hold a standard
one inch by three inch mivroscope slide. I would load a slide, fill the
boat with water (this was the tricky part since I failed to design
compensation for different knife/boat angles), cut a long ribbon of
sections--up to two inches long, lower the water level while guiding the
ribbon onto the slide, remove the slide, dry & stain as appropriate. It
might be easier to design a system for a coverslip. We used brass metal but
other materials would also work. Happy cutting!!


At 08:47 AM 3/28/02 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 29 13:18:58 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 29 Mar 2002 13:10:57 -0600
Subject: sputtering TiNi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a researcher who wishes to sputter a TiNi (50:50) binary alloy.
The plan is to place a TiNi target in a conventional sputter coater
(Technics Hummer V).

My question: can this be done with a standard sputterer? Are
different HV's needed? Different gases?

Any guidance would be appreciated before we embark on this experiment.

Thank you!!

John B.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Fri Mar 29 13:38:59 2002



From: CHEN CHEN :      cchen5-at-jhmi.edu
Date: Fri, 29 Mar 2002 14:33:34 -0500
Subject: Please subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please subscribe.

Department of Biological Chemistry
The Johns Hopkins University School of Medcine
725 N. Wolfe Street
Baltimore, MD 21205



From daemon Fri Mar 29 15:18:59 2002



From: A.P Alves de Matos :      apamatos-at-oninet.pt
Date: Fri, 29 Mar 2002 21:11:36 -0000
Subject: TEM cryosections, X-Ray mycroanalysis again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you all for the very usefull suggestions. I will study the subject in
depth in the near future taking advantage of the books suggested.
However, since I do not have time to do it now and these books are not
available to me presently, could you tell me if a cryo-transfer specimen
holder is essential for transfering the specimen to the microscope for
freeze-drying under vacum ( I will be using someone else's microscope and
acquisition of the cold stage can not be done). If so, is it possible to
improvise some device for doing the transfer to the microscope?
Suggestions about specific devices to buy?

Thank you in advance for your help


Dr. A.P. Alves de Matos
Faculty of Dental Medicine, Lisbon University
(Biologist, Ph. D.)
apamatos-at-oninet.pt

-----------------------------------------------
Previous message:

Dear Collegues

I need to do in the future some observations in cryofixed biological
specimens. In the past I have done it for immunocytochemistry, and used a
fixation step and cryoprotectant.
In this case, the sections are to be subjected to X-ray microanalysis and
fixation steps are to be avoided in order to prevent diffusion of soluble
compounds.

As far as I was able to inquire, it is possible to transfer such sections to
the microscope and freeze-dry them in the microscope itself. However I do
not have specific details on the procedure, and do not know if it is
possible to do it without special equipment.

Since I will be able to ask for new equipment till the end of the week, can
anyone give-me an idea of a pratical (if possible simple) procedure to do
this, indicating required equipment and special problems to look for?

Thanks in advance




From daemon Fri Mar 29 15:22:43 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 29 Mar 2002 15:17:19 -0600
Subject: RE: newgroup access (was RE: converting digital camera to microscope)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


DejaNews do not exist anymore. It is now Google (already mentioned).

Vladimir

}
} You can also use
}
} www.dejanews.com
}
} to look up all postings in any group.
}
} gary
}
} At 06:35 AM 3/29/2002, you wrote:
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------
} ----------.
} }
} }
} } Gary writes ...
} }
} } } I've received several questions about this.
} } }
} } } It is usenet, not the web. An ISP has newsreader
} } } service typically via news.isp.com or news.isp.net
} } } where isp is the name of the ISP.
} } }
} } } Since you are .gov, you may not have access
} } } to this if you are behind a firewall that prohibits
} } } usenet. Even though you most likely have an external
} } } ISP for main Internet access.
} } }
} } } For those who can't access the usenet, ...
} }
} } Web browser access is provided by Google.groups ... e.g.,
} } http://groups.google.com/groups?hl=en&lr=lang_en&newwindow=1&
group=sci.techn
} iques.microscopy
}
} or, for example ...
} http://groups.google.com/groups?hl=en&safe=off&group=rec.crafts.brewing
}
} or, advanced searching
} http://groups.google.com/advanced_group_search
}
} Leaving a new post or reply does require that you register ...
} http://groups.google.com/googlegroups/posting_faq.html
}
} The upside is ALL newsgroups are represented, and all messages are
} archived here (... every dumb question has already been answered ...
} possibly?). On the downside ... posts take several hours to be posted, and
} I would take the suggestion for using an e-mail alias seriously ... see
} http://groups.google.com/googlegroups/posting_faq.html#email
}
} genuinely ... michael shaffer :o)
} Avalon Peninsula, Newfoundland
} www.micro-investigations.com (in progress)




From daemon Fri Mar 29 19:00:01 2002



From: Anna Logvinova :      alogvinova-at-buckinstitute.org
Date: Fri, 29 Mar 2002 16:52:21 -0800
Subject: Streptavidin Colloidal Gold conjugate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Does anybody have a protocol to conjugate colloidal gold to streptavidin?
thanks a lot in advance,

Anna Logvinova, M.D.
Morphology Core Supervisor
Buck Institute
8001 Redwood Blvd
Novato, CA 94948
www.buckinstitute.org

(415)209-2259
alogvinova-at-buckinstitute.org


From daemon Fri Mar 29 19:03:21 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 29 Mar 2002 19:42:50 -0500
Subject: sputtering TiNi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,
I'm not terribly familiar with the Hummer, but I've seen them. They do not have a high vacuum pump, right? -Just the mechanical pump? There would be a contamination issue with just the mechanical pump, so that he shouldn't be expecting totally pure alloy containing just Ti and Ni. The TiNi isn't magnetic, so it should sputter OK. I would use a good grade of Ar as the gas and let it purge the system at a higher pressure for a while. I think on the hummer you can go into an etch mode, so that might help with hydrocarbon contamination. I am not sure what the stoichiometry of the films will be. I think that it takes some time for a steady state composition on the target to set up. The other thing that he should do is sputter for a while onto a shield. The Ti is reactive and will "getter" some of the oxygen and water out of the system so that he will not be putting down TiO2. Also, let the sample cool so that the air coming in doesn't oxidize the sample while it is still warm
from the deposition.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Friday, March 29, 2002 2:11 PM
To: Microscopy-at-sparc5.microscopy.com


We have a researcher who wishes to sputter a TiNi (50:50) binary alloy.
The plan is to place a TiNi target in a conventional sputter coater
(Technics Hummer V).

My question: can this be done with a standard sputterer? Are
different HV's needed? Different gases?

Any guidance would be appreciated before we embark on this experiment.

Thank you!!

John B.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Sat Mar 30 07:05:44 2002



From: Barbara Maloney :      bmalon01-at-fiu.edu
Date: Sat, 30 Mar 2002 07:51:23 -0500
Subject: gold coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How do you determine whether to use gold or gold-paldium for coating
your sample?
Thank you
Barb



From daemon Sat Mar 30 08:17:56 2002



From: =?iso-8859-1?Q?Rog=E9rio?= =?iso-8859-1?Q?_?= =?iso-8859-1?Q?L=FAcio?= de
Date: Sat, 30 Mar 2002 08:06:55 -0600
Subject: Niobium metal powder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers
Does anybody knows if there is a company that makes Niobium metal
powder (Grains size about few nanometers).
Thank you.
R. L. Almeida
DFMC -UNICAMP-BRASIL




From daemon Sat Mar 30 09:30:01 2002



From: Antonio Mendez Vilas :      amvilas-at-unex.es
Date: Sat Mar 30 16:17:03 2002
Subject: Call for Papers for Book on Microscopy: URL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear colleagues

Regarding the Call for Papers of the forthcoming book of
FORMATEX, "Science, Technology and Education of Microscopy:
An Overview", information on topics and submission procedure
can now be found at:

http://www.formatex.org/micro2002/callforpaper.htm

The book will be edited in a citeable form (ISBN)
and 25 reprints will be sent to authors. The
corresponding fees will be about 240 EUR (1 EUR =3D3D 0.9 US$
aprox).

If you are interested in more information on
this edition, please contact us through
micro-at-formatex.org, or directly to the editor:

A.Mendez Vilas
Physics Department
University of Extremadura
Avda. de Elvas s/n
06071 Badajoz
SPAIN

Regards

J.A. Mesa Gonzalez
FORMATEX Secretary
C/Encarnacion 3, 1st E
06001 Badajoz
SPAIN
E-mail: micro-at-formatex.org





From daemon Sat Mar 30 10:40:55 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 30 Mar 2002 11:28:56 -0500
Subject: TiNi (50:50) binary alloy sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id KAA02792
for dist-Microscopy; Sat, 30 Mar 2002 10:37:53 -0600 (CST)
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by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id KAA02783
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by granger.mail.mindspring.net with smtp (Exim 3.33 #1)
id 16rLmb-00065j-00
for Microscopy-at-MSA.Microscopy.com; Sat, 30 Mar 2002 11:32:49 -0500
To: MICROSCOPY BB {Microscopy-at-sparc5.microscopy.com}


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

John J. Bozzola wrote:
======================================================
We have a researcher who wishes to sputter a TiNi (50:50) binary alloy. The
plan is to place a TiNi target in a conventional sputter coater (Technics
Hummer V).

My question: can this be done with a standard sputterer? Are different HV's
needed? Different gases?
=======================================================
A "standard sputterer" or sputter coater operates with a rotary vane (e.g.
"mechanical") pump only and in general, does not have a true Magnetron head.
This type of system is designed to sputter low work function elements only
, and that generally includes the precious group metals (including silver).

To do just about anything else, you need a true Magnetron head and for most
elements being considered for coating, the chamber must be sufficiently free
of oxygen that a turbo pump is required.

There is a large difference in the cost of the first vs. the second, so for
ordinary gold coating applications, the coater normally found in the
laboratory is not set up to do much of anything else. Such coaters won't
even do carbon, that is why one needs a special coater for applying carbon
(more of an evaporative than sputtering) process.

I try to make this point because we are frequently encountering end users
who try taking either a chromium foil or plate, or a foil or plate of some
other composition such as stainless steel, finding that it does not work,
and then asking us what they are "doing wrong". The only thing they are
doing wrong is that they are trying to do this in a coater that was not
designed for that kind of coating. TiNi (50:50) binary alloy would fall
into this category.

Now I could be wrong about this, but since I assume you do have a vacuum
evaporator, small chunks of the alloy possibly could be evaporated from a
tungsten basket in a fairly standard EM lab type vacuum evaporator. It
would not be a sputtered coating, of course, but it might be close enough to
meet the requirements of your researcher.

Disclaimer: SPI Supplies offers both kinds of coaters on our website.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sat Mar 30 12:00:27 2002



From: peter ingram :      p.ingram-at-cellbio.duke.edu (by way of
Date: Sat, 30 Mar 2002 11:49:31 -0600
Subject: Re: TEM cryosections, X-Ray mycroanalysis again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



It is possible to freeze-dry outside the microscope. Unless you are
planning to do detailed quantitative work this probably will suffice.
You should check your grids and substrates for contamination
beforehand - sort of a "control".



However you WILL need a cryotransfer stage to freeze-dry in the
microscope (GATAN and Oxford make them but they are not cheap!) Again
I do strongly recommend you read some of the literature on this
subject; plenty is readily available on the web I am sure.

BTW, if you are planning to use someone else's microscope, please
make sure it has a cryo-protected clean vacuum, especially in the
specimen area, or else you may find your specimen contaminated and
therefore compromising your results.

Peter I.



Thank you all for the very usefull suggestions. I will study the subject in
depth in the near future taking advantage of the books suggested.
However, since I do not have time to do it now and these books are not
available to me presently, could you tell me if a cryo-transfer specimen
holder is essential for transfering the specimen to the microscope for
freeze-drying under vacum ( I will be using someone else's microscope and
acquisition of the cold stage can not be done). If so, is it possible to
improvise some device for doing the transfer to the microscope?
Suggestions about specific devices to buy?

Thank you in advance for your help


Dr. A.P. Alves de Matos
Faculty of Dental Medicine, Lisbon University
(Biologist, Ph. D.)
apamatos-at-oninet.pt

-----------------------------------------------
Previous message:

Dear Collegues

I need to do in the future some observations in cryofixed biological
specimens. In the past I have done it for immunocytochemistry, and used a
fixation step and cryoprotectant.
In this case, the sections are to be subjected to X-ray microanalysis and
fixation steps are to be avoided in order to prevent diffusion of soluble
compounds.

As far as I was able to inquire, it is possible to transfer such sections to
the microscope and freeze-dry them in the microscope itself. However I do
not have specific details on the procedure, and do not know if it is
possible to do it without special equipment.

Since I will be able to ask for new equipment till the end of the week, can
anyone give-me an idea of a pratical (if possible simple) procedure to do
this, indicating required equipment and special problems to look for?

Thanks in advance


--
Peter Ingram
Sr. Physicist
Adj. Professor of Pathology,
Duke University Medical Center
Box 930319
DURHAM
NC 27708-0319

Tel: (919) 660-2695
Fax: (919) 660-2671

Website: http://152.3.167.174/AEM_LAB.html


From daemon Sat Mar 30 13:47:53 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 30 Mar 2002 11:45:46 -0800
Subject: Re: gold coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It's a choice between Au, Au/Pd and Pt. These are what
I have available. For general purpose moderate magnification
work, Au is fine. However, I us Au/Pd most often since
it provides no visible grain up to 125KX or so. For really fine
grain, Pt works well.

The other issue is whether the specimen is going to be
analyzed with EDX. If so, then I'd use Au/Pd, since it is
far up in Z to not obscure the light elements and on up
to Ga, Ge and As. Others use C but this obscures B and N.

gary g.


At 04:51 AM 3/30/2002, you wrote:

} How do you determine whether to use gold or gold-paldium for coating
} your sample?
} Thank you
} Barb



From daemon Sat Mar 30 21:13:57 2002



From: Allyn :      esxfnepmsr-at-svizzera.org
Date: Sat, 30 Mar 2002 21:09:22 -0600 (CST)
Subject: I Thought You Would Like To Know

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Mon Apr 1 08:55:55 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 1 Apr 2002 09:31:27 -0500
Subject: Re: HMDS information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 8:35 AM -0600 3/26/02, GALLEN WANG wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

****************
Hi, I have used it only on cultured cells of animal origin. I don't
have any experience with botanical samples.
Lee



--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Apr 1 09:49:01 2002



From: ldm microscopy :      ldm2-at-risc4.numis.nwu.edu
Date: Mon, 1 Apr 2002 09:46:04 -0600 (CST)
Subject: Visualizations (summary)

Contents Retrieved from Microscopy Listserver Archives
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I have put a summary of what I found at

http://risc2.numis.nwu.edu/internet/Visualizations.htm

A comment: I found very little in the way of good
visualizations for SEM, e.g. different SEM modes.

-----------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm2-at-risc4.numis.nwu.edu
http://risc2.numis.nwu.edu
-----------------------------------------------




From daemon Mon Apr 1 10:40:10 2002



From: Jian-Guo Zheng :      j-zheng3-at-northwestern.edu
Date: Mon, 1 Apr 2002 10:31:47 -0600
Subject: position open -IMMEDIATELY: Scanning Electron Microscopist (SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


POSITION OPEN - IMMEDIATELY

Scanning Electron Microscopist (SEM)


An immediate position is open for a Scanning Electron
Microscopist at the electron probe instrumentation center (EPIC) of
Northwestern University. NU's EPIC facility
(http://epic.ms.northwestern.edu) is comprised of three Hitachi SEMs
(one field emission, one variable pressure and one conventional; all
with PC acquisition and EDS systems) and one Hitachi FIB.
Duties and responsibilities include: teaching and development of
laboratories, training and assistance to users, instrumentation
development and modifications. A BS or equivalent technical training in
science/engineering discipline is required. Required skills include:
Extensive hands-on experience with SEM, related techniques and
accessories (e.g. EDS, evaporators and specimen preparation),
teaching/user training experience in materials. Familiarity with modern
electronics, computer systems and experience with vacuum systems is
required.

Please send resume, list of 3 references, with salary requirements,
electronically to:
Prof. Vinayak P. Dravid
E-mail: v-dravid-at-northwestern.edu (preferred)
Fax: (847) 467-6573

NORTHWESTERN UNIVERSITY is an equal opportunity, affirmative action
educator and employer.


*******************************************************
(Vinayak P. Dravid)
Professor, Materials Science & Engineering
Director, Electron Probe Instrumentation Center (EPIC)
Sabbatical Faculty Fellow: National Institutes of Health

2225 N. Campus Drive, 1133 MLSF
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 491-7820
E-mail: v-dravid-at-northwestern.edu
http://vpd.ms.northwestern.edu
http://epic.ms.northwestern.edu
*******************************************************



From daemon Mon Apr 1 12:30:55 2002



From: Markus F. Meyenhofer :      micro-at-superlink.net
Date: Mon, 1 Apr 2002 13:26:21 -0500
Subject: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am cleaning out my stockroom.
Free for the taking, 5 cases of virgin electrons, one size, charged, 5
billions per package, 200 packages per case in sealed cases.
First one take all.
Regards,
Markus F. Meyenhofer



From daemon Mon Apr 1 14:10:26 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Mon, 01 Apr 2002 12:01:06 -0500
Subject: Re: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 4/1/02 1:26 PM, Markus F. Meyenhofer at micro-at-superlink.net wrote:

}
} I am cleaning out my stockroom.
} Free for the taking, 5 cases of virgin electrons, one size, charged, 5
} billions per package, 200 packages per case in sealed cases.
} First one take all.
} Regards,
} Markus F. Meyenhofer
}
Dear Markus,
As it happens, I have several cases of positive ions, so I would be
definitely interested in taking the electrons off your hands. No need to
worry about shipping; just unseal the cases, and, I'm sure, the electrons
will find their way here.
Yours,
Bill tivol



From daemon Mon Apr 1 15:26:20 2002



From: Manoj Misra :      Manoj.Misra-at-unilever.com
Date: April 16, 2002
Subject: Metro. Micros. Soc. Meeting APRIL 16th

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

The next meeting of the Metropolitan Microscopy Society will be held on April
16th, 2002 at the LEO US headquarters facility in Thornwood, NY. LEO has
graciously agreed to host our meeting and to provide complimentary coffee and
lunch to all attendees.

The meeting itself will comprise a technical symposia of six presentations
covering a range of topics of interest to both photon and electron
microscopists. The talks will cover a broad range of topics including
molecular resolution via cryo -FESEM, cell imaging at the bottom ( {100nm) of
cells, quantitative X-ray analysis using ESEM, as well SEM based metrology
methods / techniques discussions.

We invite all members to attend and to inform their colleagues and fellow
workers who may also wish to attend.

Due to the nature of the corporate facility the meeting will be held at, it is
essential that members pre-register so that an attendee list can be delivered
to the security people at LEO for generation of guest badges. It will also
help us plan for lunches.

The pre-registration deadline is April 12 and can be accomplished
electronically. Please respond via email or fax to EVAN SLOW ( ESS-at-FEICO.com)
directly. A simple email note is all that’s required to pre-register. You can
then bring the required fee with
you to the meeting. For all attendees, the meeting fee, which includes lunch,
will be $20.00.

PROGRAM

Metropolitan Microscopy Society
Spring Meeting 2002


Time: 9:00 am (registration begins)

Place: LEO, One Zeiss Drive, Thornwood, NY (914) 747- 7700

Directions: {http://www.leo-usa.com/} (click visiting LEO, Thornwood, US)

9:00 - 9:45 Registration

9:45 - 10:00 Introductory Remarks (Al Sicignano).

10:00 - 10:45 "Molecular Resolution with Cryo-Field Emission SEM:
Visualization of Individual CAMs (P-selectin, GpIX/GpIB, and GpIIb/IIIa) in the
Glycocalyx of Human Platelets, Dr. Stanley L. Erlandsen, Univ. of Minnesota
Medical School, Minneapolis, MN 55455

10:45 - 11:30 "Overview of NIST Programs Related to SEM Metrology” Dr. Andras
Vladar, Nanoscale Metrology Group, NIST, Gaithersburg, MD

11:30 - 12:15 “Flashy Fireworks: Imaging at the Bottom {100 Nanometers of the
Cell, Dr. Derek Toomre, Yale University School of Medicine, New Haven, CT

12:15 - 1:00 Lunch and facility tour (included with registration - please
pre-register!)

1:00 - 1:45 “ESEM Can Produce Quantitative X-ray Results”, Dr. Charles Lyman,
Lehigh University, Bethlehem, PA

1:45 - 2:30 " Issues Affecting SEM Measurement Precision”, Al Sicignano,
Nanometrology LLC, Ardsley, NY

2:30 - 3:15 " Application Techniques Results”, David Frey, LEO, Thornwood,
NY.


M. Misra

Manoj Misra, Ph.D.
Group Leader
Advanced Imaging & Measurement
Unilever Research
Edgewater, NJ 07020
Phone: (201) 840-2702
Fax: (201) 840-8269




From daemon Mon Apr 1 15:35:08 2002



From: Jian-Guo Zheng :      j-zheng3-at-northwestern.edu
Date: Mon, 1 Apr 2002 15:28:14 -0600
Subject: position open -IMMEDIATELY: Scanning Electron Microscopist (SEM)

Contents Retrieved from Microscopy Listserver Archives
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id xma010061; Mon, 1 Apr 02 15:28:23 -0600




POSITION OPEN - IMMEDIATELY

Scanning Electron Microscopist (SEM)


An immediate position is open for a Scanning Electron
Microscopist at the electron probe instrumentation center (EPIC) of
Northwestern University. NU's EPIC facility
(http://epic.ms.northwestern.edu) is comprised of three Hitachi SEMs
(one field emission, one variable pressure and one conventional; all
with PC acquisition and EDS systems) and one Hitachi FIB.
Duties and responsibilities include: teaching and development of
laboratories, training and assistance to users, instrumentation
development and modifications. A BS or equivalent technical training in
science/engineering discipline is required. Required skills include:
Extensive hands-on experience with SEM, related techniques and
accessories (e.g. EDS, evaporators and specimen preparation),
teaching/user training experience in materials. Familiarity with modern
electronics, computer systems and experience with vacuum systems is
required.

Please send resume, list of 3 references, with salary requirements,
electronically to:
Prof. Vinayak P. Dravid
E-mail: v-dravid-at-northwestern.edu (preferred)
Fax: (847) 467-6573

NORTHWESTERN UNIVERSITY is an equal opportunity, affirmative action
educator and employer.


*******************************************************
(Vinayak P. Dravid)
Professor, Materials Science & Engineering
Director, Electron Probe Instrumentation Center (EPIC)
Sabbatical Faculty Fellow: National Institutes of Health

2225 N. Campus Drive, 1133 MLSF
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 491-7820
E-mail: v-dravid-at-northwestern.edu
http://vpd.ms.northwestern.edu
http://epic.ms.northwestern.edu
*******************************************************



From daemon Mon Apr 1 18:48:22 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 01 Apr 2002 16:54:41 -0800
Subject: Re: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill
If it'll be unsealed, all these guys immediately will find shortest way to
the ground. Perhaps, to the nearest water-pipe or so. Person, who will do
that, will enjoy a beautiful spark in that very moment before s/he
evaporated... From another hand, we have to calculate how much damage
could produce 10^9 electrons. It seems to me not much: not enough even to
fire gasoline mixture in the engine... Useless stuff. A few packages of
virgil neutrino from the big-ban would be much better: our scientists will
sell their souls for that, perhaps...

Sergey

At 12:01 PM 4/1/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Mon Apr 1 22:00:27 2002



From: earlw-at-sbcglobal.net :      earlw-at-sbcglobal.net
Date: Mon, 1 Apr 2002 13:26:21 -0500
Subject: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Virgin electrons?"

Are there any other type?

Earl


Original Message:
-----------------
} From: Markus F. Meyenhofer micro-at-superlink.net


I am cleaning out my stockroom.
Free for the taking, 5 cases of virgin electrons, one size, charged, 5
billions per package, 200 packages per case in sealed cases.
First one take all.
Regards,
Markus F. Meyenhofer


--------------------------------------------------------------------
mail2web - Check your email from the web at
http://mail2web.com/ .



From daemon Tue Apr 2 06:13:16 2002



From: Tony Bruton :      Bruton-at-nu.ac.za
Date: Tue, 02 Apr 2002 14:03:37 +0200
Subject: Ion Getter Pumps - life expectancy and service on ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All

We would appreciate input from those who have faced similar
problems......

We have been, tentatively, advised by our service agents that the IGP
on our Philips XL30 ESEM is 'nearing the end of its service life' since
we are having gun vacuum problems (so - what's new !)

The instrument is just over two years old but is regularly used in ESEM
'wet' mode. What we would like to know is;

1) What service life others have had from IGP's particularly those in
'dirty' systems ? The IGP on our 1996-installed TEM (CM120) is,
apparently, still in good health.

2) Whether such units are 'regenerated' or simply a costly total
replacement.

3) What others have experienced as symptoms of the impending failure of
an IGP ? Our system pumps down OK but the vacuum drops dramatically when
one tries to saturate the filament. There is obviously an outgassing
problem, after a service, to add to our woes.

Thank you in anticipation of your input.



Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website: www.nu.ac.za/microscopy.asp
Email: bruton-at-nu.ac.za
Postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa


From daemon Tue Apr 2 07:46:03 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Tue, 2 Apr 2002 08:38:23 -0500
Subject: RE: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ok can we be anymore nerdy? or geeky? let me know i am sure there are those
that would try.


From daemon Tue Apr 2 12:04:20 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 02 Apr 2002 09:14:57 -0800
Subject: Re: Signals from a SEM monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Kim,
That is exactly how the Quartz PCI system (sold by Hitachi) operates to
passively capture images from an SEM. To get Polaroid quality images
produced, you must also look carefully at the printer. A laser printer is
OK, but a high resolution inkjet onto glossy paper will more closely imitate
a photo.
I have been involved with Quartz Imaging as a test lab for several years.
At 03:34 PM 3/29/02 +0900, you wrote:
}
}
} Listers,
} Our CRT in XL20 SEM Polaroid died last week.
} We're trying to replace our Polaroid system by hooking up the PC data
acquisition board.
} What we're thinking of is to get the signals of vertical and horizontal
scan in the Polaroid CRT system, plus amplified analog signal of detector,
then feed those 3 signals to PC data acquisition board. If the programming
is too much, just collect the secondary electron signal with x-y position of
the monitor. Those number matrix can be processed in the Digitalmicrograph.
} Has anyone tried this setup? Or is there any commercial hardware/software
to replace the Polaroid with PC data acquisition?
}
} Young W. Kim, Ph.D.
} Research Professor
} School of Materials Science and Engineering
} Seoul National University
} Kwanak-ku Shinlim-dong San 56-1
} Seoul, Republic of Korea 151-744
}
} Tel) + 82-2-880-7977
} E-mail) ywkim-at-gong.snu.ac.kr
}
Best of luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Tue Apr 2 12:28:35 2002



From: Shuyou Li :      syli-at-northwestern.edu
Date: Tue, 2 Apr 2002 12:19:22 -0600
Subject: Job opening: Scanning Electron Microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- Please send correspondence to Prof. Dravid directly. Don't reply this email --

POSITION OPEN - IMMEDIATELY

Scanning Electron Microscopist (SEM)
An immediate position is open for a Scanning Electron
Microscopist at the electron probe instrumentation center (EPIC) of
Northwestern University. NU's EPIC facility (
{http://epic.ms.northwestern.edu/} http://epic.ms.northwestern.edu
{http://epic.ms.northwestern.edu/} ) is comprised of three Hitachi SEMs
(one field emission, one variable pressure and one conventional; all
with PC acquisition and EDS systems) and one Hitachi FIB.
Duties and responsibilities include: teaching and development of
laboratories, training and assistance to users, instrumentation
development and modifications. A BS or equivalent technical training in
science/engineering discipline is required. Required skills include:
Extensive hands-on experience with SEM, related techniques and
accessories (e.g. EDS, evaporators and specimen preparation),
teaching/user training experience in materials. Familiarity with modern
electronics, computer systems and experience with vacuum systems is
required.

Please send resume, list of 3 references, with salary requirements,
electronically to:
Prof. Vinayak P. Dravid
E-mail: v-dravid-at-northwestern.edu (preferred)
Fax: (847) 467-6573

NORTHWESTERN UNIVERSITY is an equal opportunity, affirmative action
educator and employer.



*******************************************************
(Vinayak P. Dravid)
Professor, Materials Science & Engineering
Director, Electron Probe Instrumentation Center (EPIC)
Sabbatical Faculty Fellow: National Institutes of Health

2225 N. Campus Drive, 1133 MLSF
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 491-7820
E-mail: v-dravid-at-northwestern.edu
http://vpd.ms.northwestern. {http://vpd.ms.northwestern.edu/} edu
{http://vpd.ms.northwestern.edu/}
http://epic.ms.northwestern.edu {http://epic.ms.northwestern.edu/}
*******************************************************




Shuyou

______________
Shuyou Li
Dept MSE
Northwestern Univ
2225 N Campus Dr
Evanston, IL 60208

Tel: 1-(847)491-7798(Lab); 1-(847)-869-4348(Home)
Email: syli-at-northwestern.edu; syli16-at-hotmail.com
Web: http://pubweb.northwestern.edu/~sli974




From daemon Tue Apr 2 13:25:42 2002



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Tue, 2 Apr 2002 13:16:11 -0600
Subject: Looking for a 'single crystal' of tetragonal zirconia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Listers,

Apologies for this being a bit off topic:

I'm trying to obtain a single crystal of tetragonal zirconia, at best a
chunk about 3-5 mm on an edge.

This material can be obtained by melting at high temperature of ZrO2 with
about 8 wt% Y2O3 (I have some references if you're interested). It will
crystallize first to cubic zirconia, then on cooling three orientation
variants of tetragonal zirconia form by a displacive transformation (it is
'single crystalline only by reference to the cubic phase).

If anyone out there has this and would be willing to part with it for a
price, or knows where I might obtain it, please let me know (off line).

Thanks,

Wharton Sinkler
UOP LLC
Des Plaines, IL
847-391-3878



From daemon Tue Apr 2 13:32:52 2002



From: Gruber, Tyler :      tgruber-at-phelpsd.com
Date: Tue, 2 Apr 2002 14:26:43 -0500
Subject: RE: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ok can we be anymore nerdy? or geeky? let me know i am } sure there are those
} that would try.

Yes, John, I wasn't positive, but I had hoped we lacked the potential for the capacity to induce such currently negative conduct.

Couldn't resist,

Tyler Gruber


From daemon Tue Apr 2 14:26:29 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Tue, 02 Apr 2002 12:16:50 -0500
Subject: Re: Ion Getter Pumps - life expectancy and service on ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 4/2/02 7:03 AM, Tony Bruton at Bruton-at-nu.ac.za wrote:

}
} We would appreciate input from those who have faced similar
} problems......
}
} We have been, tentatively, advised by our service agents that the IGP
} on our Philips XL30 ESEM is 'nearing the end of its service life' since
} we are having gun vacuum problems (so - what's new !)
}
} The instrument is just over two years old but is regularly used in ESEM
} 'wet' mode. What we would like to know is;
}
} 1) What service life others have had from IGP's particularly those in
} 'dirty' systems ? The IGP on our 1996-installed TEM (CM120) is,
} apparently, still in good health.
}
} 2) Whether such units are 'regenerated' or simply a costly total
} replacement.
}
} 3) What others have experienced as symptoms of the impending failure of
} an IGP ? Our system pumps down OK but the vacuum drops dramatically when
} one tries to saturate the filament. There is obviously an outgassing
} problem, after a service, to add to our woes.
}
} Thank you in anticipation of your input.
}
Dear Tony,
An IGP is like a sponge; it absorbs ions until its capacity is reached,
then becomes ineffective. The lifetime of the IGP is inversely proportional
to the ambient pressure in which it is operated, so if it has a lifetime of
one year at a given pressure, it will live 10 years if the pressure is a
decade lower. Dirty systems are, therefore, particularly bad for IGPs. One
can replace the getter, which is just two blocks of metal, without replacing
the entire pump. Regenerating the getter would mean extracting all the
accumulated ions from the metal; it's much easier just to melt the metal and
reshape it into blocks than to extract the ions (many, such as O--, will
combine with the metal and be very hard to extract). When the IGP gets near
failure, each ion which is implanted has a greater chance of knocking other
ions out of the getter, so the pumping rate gets lower, and the final vacuum
is poorer. Since our IGPs were used in a vacuum range where their predicted
lifetimes were very long, they didn't come near failure.
Yours,
Bill Tivol



From daemon Tue Apr 2 15:35:17 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 2 Apr 2002 16:23:28 -0500
Subject: RE: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No other kind, just another name. Instead of "virgin electrons", we, here,
call them "negative electrons".

Nerdy or not, here I come!!!



} ----------
} From: John Hoffpauir
} Sent: Tuesday, April 2, 2002 8:38 AM
} To: earlw-at-sbcglobal.net
} Cc: micro-at-superlink.net; microscopy-at-sparc5.microscopy.com
} Subject: RE: Announcement
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} ok can we be anymore nerdy? or geeky? let me know i am sure there are
} those
} that would try.
}
}


From daemon Tue Apr 2 18:36:31 2002



From: Kun :      kunli-at-lycosasia.com
Date: Wed, 3 Apr 2002 08:29:33 +0800
Subject: Copper grid for lift-out of FIB prepaired samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,


Viewing the FIB prepaired (lif out)1 to 2 nm thick gate oxide samples becomes more challenging to us. We think one of the reasons might be that the copper grid we use (Ted Pellar) contains one layer of formva in addition to carbon, which may degrade the image quality. The Ted Pellar grids we use are very robust for lift out, but we have never tried dissoving the formava layer. Have some of you have ever tried this and how is the result? Recommendations are appreciated on the grids that you think are good for high resolution imaging of FIB prepaired lif-out samples.

Best wishes,

Li Kun

Failure Analysis
Chartered Semiconductor Manufacturing Ltd

------------------------------------------------------------------------
cOntact -at- Lycos {http://contact.lycosasia.com}
= 20MB for email and filestore + lots of other goodies...


From daemon Tue Apr 2 20:02:55 2002



From: max.sidorov-at-amd.com
Date: Tue, 2 Apr 2002 17:55:50 -0800
Subject: RE: Copper grid for lift-out of FIB prepaired samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Kun,
You might want to switch to "pre-thin" fibbing. Or at least use holey or lacey carbon grids without formvar. See http://www.tedpella.com/supflm_html/supflmap.htm for reference.
Hope this helps,

Max Sidorov, AMD

-----Original Message-----
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers,


Viewing the FIB prepaired (lif out)1 to 2 nm thick gate oxide samples becomes more challenging to us. We think one of the reasons might be that the copper grid we use (Ted Pellar) contains one layer of formva in addition to carbon, which may degrade the image quality. The Ted Pellar grids we use are very robust for lift out, but we have never tried dissoving the formava layer. Have some of you have ever tried this and how is the result? Recommendations are appreciated on the grids that you think are good for high resolution imaging of FIB prepaired lif-out samples.

Best wishes,

Li Kun

Failure Analysis
Chartered Semiconductor Manufacturing Ltd

------------------------------------------------------------------------
cOntact -at- Lycos {http://contact.lycosasia.com}
= 20MB for email and filestore + lots of other goodies...




From daemon Wed Apr 3 02:22:38 2002



From: Richard Langford :      richard.langford-at-materials.oxford.ac.uk
Date: Wed, 3 Apr 2002 09:14:22 +0100 (BST)
Subject: Re: Copper grid for lift-out of FIB prepaired samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In message {004901c1daa6$a07e69b0$0e00000a-at-centauri} "Kun" {kunli-at-lycosasia.com} writes:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
Dear Li Kun

We normally use copper grids with 10 um sized holes and with no support membrane. We position the membranes such that they are supported by the grid bars and the area of interest is over the holes. It is relatively easy to prod and push the membranes on the grids to achieve this using the micromanipulator.

We have found that the adhesion of the membranes to these grids is very good and only very rarely does a specimen 'fall off' the grid.


Richard






}
} Dear Listers,
}
}
} Viewing the FIB prepaired (lif out)1 to 2 nm thick gate oxide samples becomes more challenging to us. We think one of the reasons might be that the copper grid we use (Ted Pellar) contains one layer of formva in addition to carbon, which may degrade the image quality. The Ted Pellar grids we use are very robust for lift out, but we have never tried dissoving the formava layer. Have some of you have ever tried this and how is the result? Recommendations are appreciated on the grids that you think are good for high resolution imaging of FIB prepaired lif-out samples.
}
} Best wishes,
}
} Li Kun
}
} Failure Analysis
} Chartered Semiconductor Manufacturing Ltd
}
} ------------------------------------------------------------------------
} cOntact -at- Lycos {http://contact.lycosasia.com}
} = 20MB for email and filestore + lots of other goodies...
}
}


From daemon Wed Apr 3 03:40:12 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Tue, 02 Apr 2002 22:40:59 -0800
Subject: Re: Copper grid for lift-out of FIB prepaired samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Li:

If your ultimate goal is getting better resolution on your FIB lift out
samples, you may want to consider final polishing with a low energy ion
milling system. We have seen some outstanding results already and are
developing more information in conjunction with our customers. If this
is of interest, please let me know and I will send you information on
the low energy ion milling technology.

You will find some of the inforamtion on our website. Type in the
keyword TL-GM1 to get to the appropiate product information.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.


Kun wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers,
}
} Viewing the FIB prepaired (lif out)1 to 2 nm thick gate oxide samples becomes more challenging to us. We think one of the reasons might be that the copper grid we use (Ted Pellar) contains one layer of formva in addition to carbon, which may degrade the image quality. The Ted Pellar grids we use are very robust for lift out, but we have never tried dissoving the formava layer. Have some of you have ever tried this and how is the result? Recommendations are appreciated on the grids that you think are good for high resolution imaging of FIB prepaired lif-out samples.
}
} Best wishes,
}
} Li Kun
}
} Failure Analysis
} Chartered Semiconductor Manufacturing Ltd
}
} ------------------------------------------------------------------------
} cOntact -at- Lycos {http://contact.lycosasia.com}
} = 20MB for email and filestore + lots of other goodies...

--
Best regards-

David

***************************************************************************************************

David Henriks TEL: 800-728-2233 (toll free in
the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail:
henriks-at-southbaytech.com

***************************************************************************************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.




From daemon Wed Apr 3 09:39:33 2002



From: Hyman, S.C. :      sch10-at-leicester.ac.uk
Date: Wed, 3 Apr 2002 16:27:56 +0100
Subject: Reichert Ultracut E Manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A big thank-you to all who responded to my request for an Ultracut E
manual, I now have said manual plus service documentation.

Many thanks,

Stefan.

S.C. Hyman
Chief Technician
The Electron Microscope Laboratory
Faculty of Medicine and Biological Sciences
Adrian Building
University of Leicester
University Road
Leicester
LE1 7RH
United Kingdom



Tel. +44 (0116) 252 3370



From daemon Wed Apr 3 10:33:59 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 3 Apr 2002 10:27:15 -0600
Subject: Pathology Anecdotes Book?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I realize this is on the edge of what the Microscopy Listserver is
for but I suspect this crowd will have more than one individual who
knows the answer. When I was in grad school, I read a couple of
books by a famous pathologist. Each book was a collection of short
stories written from the general public in which some pathological
event was featured. Some dealt with microscopy, others with more
gross anatomical or physiological aspects. I would like to use these
stories in a class but can't remember the author. I have a vague
recollection that the author had a French name. Can anyone help?
Thanks, Tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Apr 3 11:02:29 2002



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 3 Apr 2002 10:55:25 -0600
Subject: RE: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Virgin electrons - In your dreams! These electron swap meets have been
ongoing since the begining of time, or longer!

-----Original Message-----
} From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
Sent: Tuesday, April 02, 2002 3:23 PM
To: 'John Hoffpauir'
Cc: 'List-Microscopy'


No other kind, just another name. Instead of "virgin electrons", we, here,
call them "negative electrons".

Nerdy or not, here I come!!!



} ----------
} From: John Hoffpauir
} Sent: Tuesday, April 2, 2002 8:38 AM
} To: earlw-at-sbcglobal.net
} Cc: micro-at-superlink.net; microscopy-at-sparc5.microscopy.com
} Subject: RE: Announcement
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} ok can we be anymore nerdy? or geeky? let me know i am sure there are
} those
} that would try.
}
}


From daemon Wed Apr 3 11:12:45 2002



From: Heeschen, Bill (WA) :      WAHEESCHEN-at-dow.com
Date: Wed, 3 Apr 2002 07:56:25 -0600
Subject: Microscopist Position Available: The Dow Chemical Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopist Position with The Dow Chemical Company

Educational requirements
Ph.D. or M.S. in Materials Science, Chemistry or related physical science
field.

Job Location
Position exists in South Charleston, West Virginia

Job description
Lead the microscopy effort at Dow's South Charleston, West Virginia site.
Use light microscopy, scanning electron microscopy, transmission electron
microscopy and atomic force microscopy to provide solutions to materials
characterization issues primarily related to the catalysis field.

The candidate must have hands-on experience with a broad range of microscopy
techniques, working knowledge of associated techniques such as XPS, SIMS,
XRD, XRF and surface area and porosimetry analysis and the ability to lead
Dow into new areas of catalyst characterization. The candidate will be
expected to work independently on projects and also as part of
multifunctional teams consisting of other scientists and technologists. The
candidate must have good manual dexterity and a willingness to learn. The
job will require good communication (English is job-site language) and
interpersonal skills. Opportunities will exist for travel to other Dow
sites as well as technical meetings/training. Other requirements include
participation in and support of ongoing safety and quality performance
programs.

List of goals critical for this job position
1. Develop partnerships with University and government labs to access unique
technology.
2. Become an active member/participant on new product/catalyst development
teams.
3. Partner with colleagues at other Dow sites to gain access to technology
not practiced at the South Charleston site but which is relevant to the
analytical needs of researchers at that site.
4. Actively participate in global technology teams.
5. Develop or implement new characterization technologies as driven by
market needs.
6. Support the local site production and R&D needs primarily using
microscopy based tools.
7. Develop or implement new technology to meet current and perceived future
customer needs.


For further information call or email one of these Dow contacts.

Ralph Guerra
Freeport, Texas
(979) 238-1228
rguerra-at-dow.com

John Blackson
Midland, Michigan
(989) 636-6316
blacksonjohn-at-dow.com

Ghaleb Salaita
South Charleston, West Virginia
(304) 747-5171
salaitgn-at-dow.com

Dow is a leading science and technology company that provides innovative
chemical, plastic and agricultural products and services to many essential
consumer markets. With annual sales of $30 billion, Dow serves customers in
more than 160 countries and a wide range of markets that are vital to human
progress, including food, transportation, health and medicine, personal and
home care, and building and construction, among others. Committed to the
principles of sustainable development, Dow and its 50,000 employees seek to
balance economic, environmental and social responsibilities


Apply online to:
www.careersatdow.com. Job # 0100043, Title: Microscopist
Only those selected for an interview will be
contacted.


Best Regards,
William A. Heeschen
Microscopy, Digital Imaging
The Dow Chemical Company


From daemon Wed Apr 3 13:35:35 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 3 Apr 2002 13:11:29 -0600
Subject: Re: Pathology Anecdotes Book?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Tom,

I believe you are referring to Berton Roueche (acute accent over the
last e), author of such books as: The Medical Detectives, volumes I,
II; The Orange Man, Eleven Blue Men, etc. Publishers are
Little-Brown, Times Books, Dutton, etc.

Truly engrossing works with definite practical applications in
numerous disciplines.

Cheers,

John B.



} I realize this is on the edge of what the Microscopy Listserver is
} for but I suspect this crowd will have more than one individual who
} knows the answer. When I was in grad school, I read a couple of
} books by a famous pathologist. Each book was a collection of short
} stories written from the general public in which some pathological
} event was featured. Some dealt with microscopy, others with more
} gross anatomical or physiological aspects. I would like to use
} these stories in a class but can't remember the author. I have a
} vague recollection that the author had a French name. Can anyone
} help? Thanks, Tom
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Apr 3 13:56:31 2002



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Wed, 3 Apr 2002 13:49:27 -0600
Subject: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Listers,

We are working on setting up automated image analysis for particle size from
SEM images of powders (SE images). By automated, I mean with as little
interactive input as possible. We'd like to get reasonably accurate numbers
describing particle sizes, shape features, and statistical descriptions of
their distributions.

I am aware of John Russ's book and CD of Photoshop plugins, but I'm doubtful
that this will provide the automation we're interested in.

Could I get some input on what others have found to be options here -
recommendations, warnings etc.?

Thanks,

Wharton Sinkler
UOP LLC
847-391-3878



From daemon Wed Apr 3 14:14:03 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Wed, 3 Apr 2002 15:07:45 -0500
Subject: RE: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hey, lighten up a little! Even Scientists get to have
a little fun, once in a while.

Darrell

"John Hoffpauir" {John.Hoffpauir-at-mail.tju.edu} on 04/02/2002 08:38:23 AM

To: earlw-at-sbcglobal.net
cc: micro-at-superlink.net, microscopy-at-sparc5.microscopy.com


ok can we be anymore nerdy? or geeky? let me know i am sure there are those
that would try.






From daemon Wed Apr 3 18:47:46 2002



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 3 Apr 2002 18:39:34 -0600
Subject: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wharton,
Depending on the particle material, size, shape, and even the substrate, SE
images can be very challenging to enable "automatic" recognition of entire
particles. I have found that when I am doing tasks that are more of a
"manual" nature, SE images work very well. When I need more "automatic"
recognition, I try to start with BE images. This is not always practical,
and does not guarantee good results, but it is usually a good starting point
for me. The bottom line for me in this application is to maximize contrast
between substrate and particles, and minimize particle contact. Not always
easy, and sometimes not practical. If your SE images can do that, great;
otherwise it may be worth a trip back to the sample prep drawing board so
that you can achieve the contrast and dispersion necessary.

I use older software that works good but is no longer available, so I'm no
help there. But I find that most of my applications don't ever achieve the
status of "automatic", so I need to rely heavily on a some manual fixes,
using the wonderful tools in these software packages.

I also will be interested to see how successful others have been while
running on auto-pilot.

Good Luck,

Brad Huggins
BP Chemicals
Naperville, IL


-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Wednesday, April 03, 2002 1:49 PM
To: Microscopy-at-sparc5.microscopy.com
Cc: Galloway, Douglas



Dear Listers,

We are working on setting up automated image analysis for particle size from
SEM images of powders (SE images). By automated, I mean with as little
interactive input as possible. We'd like to get reasonably accurate numbers
describing particle sizes, shape features, and statistical descriptions of
their distributions.

I am aware of John Russ's book and CD of Photoshop plugins, but I'm doubtful
that this will provide the automation we're interested in.

Could I get some input on what others have found to be options here -
recommendations, warnings etc.?

Thanks,

Wharton Sinkler
UOP LLC
847-391-3878



From daemon Wed Apr 3 20:57:32 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Wed, 3 Apr 2002 20:52:44 -0600
Subject: Manual for Polaron E6100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone help me. I'm looking for a manual or copy of a manual for a
Polaron E6100 vacuum evaporator/sputter coater. It uses the E5350 Sputter
controller. I would be happy to pay for copying expense or if someone can
direct me to a vendor who may be able to provide the instruction manual we
would be willing to buy one.

Damian Neuberger, PhD
Department of Applied Sciences
Baxter Healthcare Corp.
P.O. Box 490
Rt. 120 & Wilson Rd
Round Lake, IL 60073
tel: 847.270.5888
fax: 847.270.5897
damian_neuberger-at-baxter.com




From daemon Wed Apr 3 21:19:09 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Wed, 3 Apr 2002 22:12:32 -0500 (EST)
Subject: RE: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wharton,

As Brad said, BSE images do work very well for particle analysis.
Having images that are easily binarized (or already binary by taking high
contrast BSE images) will probably be key to your goal of incurring very
little post-processing. I used to do particle analysis by 1) achieving a
good dispersion (as Brad mentioned) so that few particles were touching
each other, but with enough particles in the sample so that a large number
were represented in each image, and 2) taking very high contrast BSE images on
a low Z background such as carbon tape.

If you happen to have a PGT x-ray system, I used to use their image
analysis software to analyze the final images and it worked very well.
Other EDS companies may offer similar software. I'd hazard a guess that
NIH Image (or the PC version, Scion Image) may also do what you need
(maybe with some help from a macro). NIH Image is free and the people on
the listserver are extremely helpful. http://rsb.info.nih.gov/nih-image/

Hope that is helpful.

Best regards,

Angela

-----------------------------------------
**Please note new department name**

Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------

On Wed, 3 Apr 2002, Huggins, Bradley J wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Wharton,
} Depending on the particle material, size, shape, and even the substrate, SE
} images can be very challenging to enable "automatic" recognition of entire
} particles. I have found that when I am doing tasks that are more of a
} "manual" nature, SE images work very well. When I need more "automatic"
} recognition, I try to start with BE images. This is not always practical,
} and does not guarantee good results, but it is usually a good starting point
} for me. The bottom line for me in this application is to maximize contrast
} between substrate and particles, and minimize particle contact. Not always
} easy, and sometimes not practical. If your SE images can do that, great;
} otherwise it may be worth a trip back to the sample prep drawing board so
} that you can achieve the contrast and dispersion necessary.
}
} I use older software that works good but is no longer available, so I'm no
} help there. But I find that most of my applications don't ever achieve the
} status of "automatic", so I need to rely heavily on a some manual fixes,
} using the wonderful tools in these software packages.
}
} I also will be interested to see how successful others have been while
} running on auto-pilot.
}
} Good Luck,
}
} Brad Huggins
} BP Chemicals
} Naperville, IL
}
}
} -----Original Message-----
} } From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
} Sent: Wednesday, April 03, 2002 1:49 PM
} To: Microscopy-at-sparc5.microscopy.com
} Cc: Galloway, Douglas
} Subject: Automated image analysis for particle size from SEM images
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear Listers,
}
} We are working on setting up automated image analysis for particle size from
} SEM images of powders (SE images). By automated, I mean with as little
} interactive input as possible. We'd like to get reasonably accurate numbers
} describing particle sizes, shape features, and statistical descriptions of
} their distributions.
}
} I am aware of John Russ's book and CD of Photoshop plugins, but I'm doubtful
} that this will provide the automation we're interested in.
}
} Could I get some input on what others have found to be options here -
} recommendations, warnings etc.?
}
} Thanks,
}
} Wharton Sinkler
} UOP LLC
} 847-391-3878
}
}
}



From daemon Wed Apr 3 21:46:56 2002



From: Edward_Principe-at-amat.com
Date: Wed, 3 Apr 2002 19:40:27 -0800
Subject: Re: Copper grid for lift-out of FIB prepaired samples on Gate Oxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Li,

The quality of the final TEM sample, no matter what method is used to thin,
depends most strongly on the final surface condition (roughness included).
This means that the best TEM samples for a gate oxide application should
have a final step that produces very thin cross sections of minimal
roughness. FOR TEM, this should be less than ~60A if you don't want the
dark band at the oxide/substrate interface caused by extinction
oscillations. Depending upon your objective, you will need to be very
cautious. FIB (pre-thinned or lift-out) will generally not achieve this
objective alone so you need to follow on with low energy low angle ion
milling or chemical thinning where applicable. Pre-thinned and lift-out
FIB samples are not ideally suited for following with ion milling for
super resolution work, but the company OmniProbe makes a micro-manipulater
that goes into one of the gas injection ports and this allows you to "weld"
the lift out specimen onto a holder inside the FIB (
http://www.omniprobe.com/). Since there is no carbon/formvar grid (which
you can't use for the best results, but a light carbon deposition on the
bottom of the sample is needed), you can do post ion milling. I am also
personally working on doing the final milling in-situ inside the SEM with a
low energy ion argon gun (~250eV-500eV) on the free standing sample. If
you are trying to obtain highest accuracy for absolute thickness using TEM
on a 20A gate oxide there are several issues you will want to consider.
You may require a TEM with better performance than you have available,
again depending upon your objective. For absolute thickness and
consistency, STEM is more forgiving of the sample preparation (see D.
Muller's papers) and the methods to determine thickness can be applied in
most cases more consistently than with TEM. The two techniques (TEM vs
STEM) may also yield different results as STEM is more sensistive to both
chemical contrast at the interface and physical roughness, often thought of
as very similar but originating from either physical or chemical disorder
at the interface. There are entirely separate problematic issues when
working on high-K materials that can be hygroscopic (again see Muller).

Some of these very issues you are struggling with have been the topic of
some recent presentations by myself and others whom I have had the good
fortune to work with, including world class TEM and STEM experts.

This material was presented on 20A nitrided gate oxide analyzed by TEM/STEM
and XPS at the recent AVS in San Francisco Oct28-Nov2 2001
"Pushing the Limits of Nitrided Gate Oxide Materials: The Materials
Characterization Role of TEM/STEM PEELS and XPS"
E. Principe, A. Hegedus, C. Kisielowski, C. Song, B. Freitag, D. Hubert, T.
Fliervoet, J. Gibson, J. Moulder, D. Watson

I am also fortunate to collaborate on a paper with Christian Kisielowski
(excellent TEM expert at National Center of Electron Microscopy in
Berkeley), Alain Diebold, B. Foran (both of SEMATECH). David Muller (Bell
Labs,Lucent), S. Pennycook (Oak Ridge), E. Principe (Applied Materials), S.
Stemmer (Rice University) that is in preparation called "Thin Dielectric
Film Thickness Determination by Advanced Transmission Electron Microscopy"

The purpose of this paper is to address many of the issues to bear in mind
in obtaining quantitative information on these very thin layers by TEM/STEM
and PEELS, as well as XPS.

So, you are not alone in your struggle and it is important to
remember.......you will get "an answer" from whatever TEM sample you
produce....but is it the correct answer for your purpose?? Paraphrasing
John Henry Scott (NIST) as he put it in one of his lucid papers on the
subject, the TEM images (interferograms really) are very impelling and thus
you want to believe them. But, he showed you can have over 3.3A
statistical error in the gate oxide measurement.....splitting hairs (or
about 1/30,000th of a hair) you say, but when the gate is only 20A in the
first place this is significant. Our work got the accuracy down to
~0.4-0.7A using focal series acquisition and exit wave reconstruction on
the world's highest resolution TEM at NCEM (I believe it still has the
record with ~80pm resolution). But, that is not available for everyone
although Christian has taken it a long way toward become nearly routine. I
believe I am safe to say he has done more of these FSR/ EWR than anyone
else in the world.

Good Luck!

Ed Principe
Member of Technical Staff
Defect & Thin Film Characterization Laboratory
Applied Materials





From daemon Thu Apr 4 02:09:25 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Thu, 04 Apr 2002 09:58:57 +0200
Subject: Re: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
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Hi,

There are several image analysis packages available and most of them
have some sort of macro language to allow you to build your own
automated image analysis routines. I believe each of the packages has
its advantages and disadvantages. The more widely used the easier you
can exchange information and even routines with other users. It is
always wise to subscribe to a user mailing list, as someone might
already have written a macro for your analysis.

I believe one of the more popular packges for EM is
DigitalMicrograph[tm] from Gatan.

http://www.gatan.com/imaging/dig_micrograph.html

Soft-Imaging distributes the analySIS software which is also very nice
for EM imaging:

http://www.soft-imaging.com/index_h.htm

Image-Pro Plus from MediaCybernetics is also very popular, both in the
life sciences as in material sciences:

http://www.mediacy.com/

I know about some more packages and compiled a list on my webpage on
microscopy and imaging:

http://ourworld.compuserve.com/homepages/pvosta/pcrimg.htm

In general if you want to do automated image analysis, take a look at
the excellent article of Prof. I. T. Young (T.U.Delft) about
quantitative microscopy.

http://www.ph.tn.tudelft.nl/People/young/manuscripts/QM/QM.html

Best regards,

Peter

P.S. I have no commerical interest or relation with any of the companies
mentioned here. I use my own Unix software for automated microscopy,
based on C-libraries ;-)

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)

Cipalstraat 3
B-2440 Geel
Belgium

tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

=========================================================
"Sinkler, Wharton" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers,
}
} We are working on setting up automated image analysis for particle size from
} SEM images of powders (SE images). By automated, I mean with as little
} interactive input as possible. We'd like to get reasonably accurate numbers
} describing particle sizes, shape features, and statistical descriptions of
} their distributions.
}
} I am aware of John Russ's book and CD of Photoshop plugins, but I'm doubtful
} that this will provide the automation we're interested in.
}
} Could I get some input on what others have found to be options here -
} recommendations, warnings etc.?
}
} Thanks,
}
} Wharton Sinkler
} UOP LLC
} 847-391-3878


From daemon Thu Apr 4 06:20:18 2002



From: priscilla.simonis :      priscilla.simonis-at-fundp.ac.be
Date: Thu, 04 Apr 2002 14:10:33 +0200
Subject: SEM polymerization

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I am new in the SEM field and have some problems with contamination seen in
my images:

When I do an image of my sample (gold), I have a quite nice image but when
I take an image at a larger scale after the first one, I clearly see a
"square" of (I suppose) polymerized stuff exactly where I did the zoom.
In other word, each time I take an image with the SEM, there is something
deposited on my sample. This white square indicates me that there is
something on the borders of the scanned area but I do not know if I deposit
stuff only there or on the whole scanned area.

Could somebody explain me why is this happening? Is it some polymerization
of a liquid contaminant layer due to the beam? could it be some
contamination from a bad vacuum and not a layer? is it the same?
Do you know some articles explaining such contamination ?
How could I know what kind of stuff it is?

thank you very much for your help.

priscilla simonis



From daemon Thu Apr 4 08:28:08 2002



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 4/3/02 6:39 PM
Subject: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wharton,

Bradley has given you lots of good informtion. Further, image
acquisition needs to be as standard as possible as well as maximizing
contrast between the particles and background. Thresholding these new
images can be an art in itself, which is why standard acquisition is
so important. Once a threshold setting has been selected, usually
through some trial and error, the same parameters of thresholding or
any other image optimization will/should be used on all images in a
given study/experiment. Photoshop with IPTK is very capable of doing
this with batch processing and the "actions" macros. The data output
in ASCII format is the least favorite aspect of this process. For
particle sizing carbon black aggregates, we use Optimas and macros
that output data to Excel.

Chuck Butterick
Engineered Carbons, Inc.


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Wharton,
Depending on the particle material, size, shape, and even the substrate, SE
images can be very challenging to enable "automatic" recognition of entire
particles. I have found that when I am doing tasks that are more of a
"manual" nature, SE images work very well. When I need more "automatic"
recognition, I try to start with BE images. This is not always practical,
and does not guarantee good results, but it is usually a good starting point
for me. The bottom line for me in this application is to maximize contrast
between substrate and particles, and minimize particle contact. Not always
easy, and sometimes not practical. If your SE images can do that, great;
otherwise it may be worth a trip back to the sample prep drawing board so
that you can achieve the contrast and dispersion necessary.

I use older software that works good but is no longer available, so I'm no
help there. But I find that most of my applications don't ever achieve the
status of "automatic", so I need to rely heavily on a some manual fixes,
using the wonderful tools in these software packages.

I also will be interested to see how successful others have been while
running on auto-pilot.

Good Luck,

Brad Huggins
BP Chemicals
Naperville, IL


-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Wednesday, April 03, 2002 1:49 PM
To: Microscopy-at-sparc5.microscopy.com
Cc: Galloway, Douglas


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Dear Listers,

We are working on setting up automated image analysis for particle size from
SEM images of powders (SE images). By automated, I mean with as little
interactive input as possible. We'd like to get reasonably accurate numbers
describing particle sizes, shape features, and statistical descriptions of
their distributions.

I am aware of John Russ's book and CD of Photoshop plugins, but I'm doubtful
that this will provide the automation we're interested in.

Could I get some input on what others have found to be options here -
recommendations, warnings etc.?

Thanks,

Wharton Sinkler
UOP LLC
847-391-3878






From daemon Thu Apr 4 09:57:19 2002



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Thu, 04 Apr 2002 10:41:48 -0500
Subject: RE: Ion Getter Pumps - life expectancy and service on ESEM

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Hi Tony,

Bill explained getters much better than I could have, but I
can offer some XL30 experience for reference. I gather you
have the LaB6 column with single IGP (?) I run the
ESEM-FEG, but less time in ESEM than you (perhaps 50/50).
Our system is about 3 years running; I had one IGP bad on
delivery but no problems since. The lower IGP in the FEG
column lives at about 1-2e-7 and is still going strong. I
assume this one will give out first. I don't know the LaB6
column but I think I'd be a bit concerned about 2 years'
lifetime, and particularly the outgassing on saturation.

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Tuesday, April 02, 2002 12:17 PM, Bill & Sue Tivol
[SMTP:wtivol-at-earthlink.net] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
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}
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}
}
} on 4/2/02 7:03 AM, Tony Bruton at Bruton-at-nu.ac.za wrote:
}
} }
} } We would appreciate input from those who have faced
} } similar
} } problems......
} }
} } We have been, tentatively, advised by our service
agents
} } that the IGP
} } on our Philips XL30 ESEM is 'nearing the end of its
} } service life' since
} } we are having gun vacuum problems (so - what's new !)
} }
} } The instrument is just over two years old but is
} } regularly used in ESEM
} } 'wet' mode. What we would like to know is;
} }
} } 1) What service life others have had from IGP's
} } particularly those in
} } 'dirty' systems ? The IGP on our 1996-installed TEM
} } (CM120) is,
} } apparently, still in good health.
} }
} } 2) Whether such units are 'regenerated' or simply a
} } costly total
} } replacement.
} }
} } 3) What others have experienced as symptoms of the
} } impending failure of
} } an IGP ? Our system pumps down OK but the vacuum drops
} } dramatically when
} } one tries to saturate the filament. There is obviously
} } an outgassing
} } problem, after a service, to add to our woes.
} }
} } Thank you in anticipation of your input.
} }
} Dear Tony,
} An IGP is like a sponge; it absorbs ions until its
} capacity is reached,
} then becomes ineffective. The lifetime of the IGP is
} inversely proportional
} to the ambient pressure in which it is operated, so if it
} has a lifetime of
} one year at a given pressure, it will live 10 years if
the
} pressure is a
} decade lower. Dirty systems are, therefore, particularly
} bad for IGPs. One
} can replace the getter, which is just two blocks of
metal,
} without replacing
} the entire pump. Regenerating the getter would mean
} extracting all the
} accumulated ions from the metal; it's much easier just to
} melt the metal and
} reshape it into blocks than to extract the ions (many,
} such as O--, will
} combine with the metal and be very hard to extract).
When
} the IGP gets near
} failure, each ion which is implanted has a greater chance
} of knocking other
} ions out of the getter, so the pumping rate gets lower,
} and the final vacuum
} is poorer. Since our IGPs were used in a vacuum range
} where their predicted
} lifetimes were very long, they didn't come near failure.
} Yours,
} Bill Tivol



From daemon Thu Apr 4 10:23:21 2002



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Thursday, April 04, 2002 4:45 AM
Subject: SEM polymerization

Contents Retrieved from Microscopy Listserver Archives
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Dear Priscilla:

The contamination layer is formed by the electron beam making hydrocarbon
ions that then follow the electron beam to the sample surface where they
form a polymer goo. Thus a black square forms in the scanned area.

The hydrocarbons are either on the surface of your specimen or you have a
contaminated chamber with a high partial pressure of hydrocarbons. These
hydrocarbons are either built into the chamber by manufacturer by accident,
come in via dirty specimens, or are from vacuum pump oil backstreaming.

We make systems to remove contamination from SEMs. Visit our Web Site at
www.SEMCLEAN.com for details. I am behind on posting some new articles on
contamination and its removal at the web site, but I do have references
which I will list in another posting this evening if other listers don't
help you today. (I have to leave now to install a system today).

Ron Vane
XEI Scientific
3124 Wessex Way
Redwood City, CA 94061
650-369-0133

-----Original Message-----
} From: priscilla.simonis {priscilla.simonis-at-fundp.ac.be}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Thu Apr 4 10:40:08 2002



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Thu, 04 Apr 2002 10:37:37 -0800
Subject: Re: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
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Wharton,

This kind of problem can be approached many different ways, ranging from
semi-manual location and measurement of particles using a general-purpose SEM to
highly automated systems optimized for precisely the kind of application you
describe It sounds like you are setting up a "production" type of process
with simplicity and reliability as major objectives, and are asking about the
latter.

There are two distinctly different ways of automating a SEM for particle
analysis. One is to capture an image and then analyze the size and shape of the
particle from the pixels in the image. Various vendors sell packages of this
type which can be installed on most any SEM with a digital imaging interface
(this is what you would get with a Photoshop plugin). The other way of
analyzing particles is "on the fly" -- in this technique (pioneered by Eugene
White at Penn State University in the '70s), the SEM beam is rastered in a
relatively coarse pattern until a particle is detected against the substrate.
No "image" is collected -- rather the software guiding the beam looks for
particles on a an individual basis. Once a particle is detected, the beam is
then rapidly moved in a finer-stepped pattern to determine the shape
parameters. If the particle meets analysis criteria of size and shape, it is
typical to then put the beam in the center of the particle and collect an EDS
spectrum for characterization. The particle is then categorized according to
pre-determined critera. This on-the-fly technique is demonstrably much faster
and more accurate than the image-capture variety -- for high-throughput
applications, it is really the only way to go (obviously an opinion, but one I
have no trouble defending). A key reason that this is so is because the system
uses pixel spacing optimally -- larger steps when just searching for particles
above a certain size (think of a seive) and then smaller steps for accuracy in
size/shape determination. There is also a lot less time wasted in moving
high-resolution images around. There is also a lot less problem with doing
analysis of small particles -- the EDS analysis is done on the particle at the
time it is located and drift effects are minimized.

Though in principle this latter kind of on-the-fly analysis can be done with
appropriate software installed on any SEM that has an interface that permits
"beam steering", the fact is that it helps a lot if the hardware is designed
with this kind of application in mind. Since the goal is to run fast and
reliably with no operator attention, details are important and one wants every
component of the system to be optimized for the task.

Disclaimer -- our firm manufactures a production-oriented SEM which is optimized
for this kind of application and I have an obvious vested interest in promoting
this kind of automated analysis.

BTW -- I would also echo the respondent who observed that BSE signals are
generally preferable for automated analysis.

Fred Schamber
Chief Technology Officer
ASPEX LLC


"Sinkler, Wharton" wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers,
}
} We are working on setting up automated image analysis for particle size from
} SEM images of powders (SE images). By automated, I mean with as little
} interactive input as possible. We'd like to get reasonably accurate numbers
} describing particle sizes, shape features, and statistical descriptions of
} their distributions.
}
} I am aware of John Russ's book and CD of Photoshop plugins, but I'm doubtful
} that this will provide the automation we're interested in.
}
} Could I get some input on what others have found to be options here -
} recommendations, warnings etc.?
}
} Thanks,
}
} Wharton Sinkler
} UOP LLC
} 847-391-3878




From daemon Thu Apr 4 11:29:09 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 4 Apr 2002 09:21:39 -0800 (PST)
Subject: Re: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
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Howdy,

Keep in mind that Photoshop is easily scriptable and can become very
automated. However, some of the tools in Photoshop are not selectable and
this forces you to stop and manually select the next tool in order for the
script to continue on its merry way. I don't if macros or scripts in other
programs have the same problem.

Bob
U of Washington
Seattle

On Wed, 3 Apr 2002, Sinkler, Wharton wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear Listers,
}
} We are working on setting up automated image analysis for particle size from
} SEM images of powders (SE images). By automated, I mean with as little
} interactive input as possible. We'd like to get reasonably accurate numbers
} describing particle sizes, shape features, and statistical descriptions of
} their distributions.
}
} I am aware of John Russ's book and CD of Photoshop plugins, but I'm doubtful
} that this will provide the automation we're interested in.
}
} Could I get some input on what others have found to be options here -
} recommendations, warnings etc.?
}
} Thanks,
}
} Wharton Sinkler
} UOP LLC
} 847-391-3878
}
}
}



From daemon Thu Apr 4 13:52:18 2002



From: Feng Peng :      fepeng-at-mailbox.syr.edu
Date: Thu, 4 Apr 2002 14:29:51 -0500
Subject: RE: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
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Hello,

We have been using the Image Analysis System (by Advanced Research Instruments
Corp. in Colorado) interfaced with SEM to conduct the online image analysis
for particles. This system can measure particle size, area, perimeter, width,
and length, etc. In its summary subroutine, you can ask to display the size
distribution in histogram. But for detailed or desired statistical analysis,
you'll have to use offline data processing program to read in raw data and
perform the analysis.

Hope this information can be of help.

} ===== Original Message From "Sinkler, Wharton" {WSinkler-at-uop.com} =====
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

:-) :-) :-) :-) :-) :-)
***********************
Peng, Feng
Graduate Student
Department of Chemistry
SUNY-ESF
1 Forestry Drive
(315)470-4740(O)
(315)424-0187(H)
fepeng-at-syr.edu



From daemon Thu Apr 4 14:18:45 2002



From: John Minter :      john.minter-at-kodak.com
Date: Thu, 4 Apr 2002 15:12:53 -0500
Subject: RE: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wharton Sinkler asked ...
We are working on setting up automated image analysis
for particle size from SEM images of powders...
Could I get some input on what others have found to be
options here - recommendations, warnings etc.?

Here at Kodak we have developed several generations of systems
to do this kind of analysis. Our current system is built around
the "analySIS" package by Soft Imaging System (their site is
http://www.soft-imaging.de ). We have evaluated secondary
electron imaging (SEI), backscattered electeon imaging (BEI),
and scanning transmission electron imaging (STEI). Many of
our measurements are made on a Cambridge (LEO) 360 SEM with
a STEM detector. We use the same package on several other
microscopes...

We calibrate the pixel size of our images with a custom
sample with a square array of holes with spacings 0.1, 0.25,
1.0, and 5.0 microns. These have been certified by the National
Physical Laboratory to an accuracy of +/- 2%. Our long term
precision in spatial calibration of our "workhorse" microscope
(established from a control chart) is 0.12%. The long term precision
of the mean size measurement(measured by STEI) on this microscope
from a "control" sample typical of what we measure (established from
a control chart) is 1.2%. This includes all sources of variability
(sampling, imaging conditions, and image analysis). We required years of
work to reach these limits.

We discovered that the mean diameter of electron dense materials
approximately 1 micron in diameter were 5% larger when meaured
with SEI than with STEI. We attribute this to over-detection of
the particles because of the enhanced secondary electron emission
at the edge. We also noticed a 5% lower mean diameter measured from BEI.
Generally the resolution of BEI was worse than STEI or SEI because
we needed to use larger probes to obtain the bacscattered images.

Proper detection of the particles was highly dependent on proper
gray level threshold determination. We experimented with several
automated thresholding algorithms before settling on one variation
of the analySIS autothreshold routine.

Proper handling of agglomerates is essential for obtaining reliable
results. "Primary particles" of most of our materials of interest
are imaged as convex objects. This property permits us to use
several shape factor discriminants to detect agglomerates. Most
image analysis packages implement at least some of these. The
best known of these discriminates is "circularity" (defined as
4 pi area / perimeter**2.) A related measure that we have found to
be more generally useful is the ratio of particle area to convex area
(lower for agglomerates). These are both implemented in analySIS
and many other packages. Still better is a measurement of the maximum
perpendicular distance between the particle boundary and the convex hull
(think of a rubber band around the particle). The maximum perpendicular
distance is larger for agglomerates. We wrote a custom module for analySIS
to measure this and obtain the most reliable agglomerate detection that
we have been able to obtain.

We tend to remove agglomerates from the size measurements
and keep track of the area fraction of agglomerates to be
certain that we are not rejecting too large a fraction of material.
We also use a guard frame to correct for bias of the distribution
by selectively rejecting larger particles that have a higher
probability of touching the image borders.

The package "analySIS", like others, has significant automation capabilities
"out of the box." The package can process series of images from
the microscope with stage control or file series. We have extended
the default capabilites to permit use of many custom functions.

AnalySIS, like most similar packages, permits data to be written to an Excel
workbook. The Visual Basic for Applications (VBA) language
that is part of the Office 97 and 2000 suites permit us to automate
all of our data reduction. Because Office is so ubiquitous, our
(internal) clients really like getting their results in Excel
workbooks.

I would like to hear of the experiences of others....

Disclaimer: I have no financial interest in Soft Imaging System or
Microsoft. I'm simply a satisfied customer and enjoy programming....



Best Regards,

John Minter, Ph. D.
Eastman Kodak Co.
Analytical Technology Division
Rochester, NY 14650-2152
Phone: (585) 722-3407
FAX: (585) 477-9303



From daemon Thu Apr 4 14:37:12 2002



From: Xianglin Li :      Xianglin_Li-at-student.uml.edu
Date: Thu, 04 Apr 2002 15:30:45 -0500
Subject: About III-V Compound Semiconductor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I heard that there is a free journal about III-V Compound
Semiconductor. Could anybody give me some information about this?

Thank you very much!

Xianglin

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Thu Apr 4 14:49:50 2002



From: Bob Harris :      bharris-at-micro.uoguelph.ca
Date: Thu, 4 Apr 2002 15:42:58 -0500
Subject: Parts for Zeiss Photomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:

We are looking for the control boxes for the fluorescence unit of
our Zeiss Photomicroscope. The only person capable of getting it
back in really great shape is going to retire in a few years and we'd
like to get the fluorescence unit of this grand old microscope
operating before he goes. If anyne knows where we might get these
parts we'd be most grateful to hear. Thanks. bob
Bob Harris
NSERC Regional STEM Facility
Dep't of Microbiology
University of Guelph
Guelph Ontario
Canada N1G 2W1
Phone: 519-824-4120 ext 6409
Fax: 519-837-1802


From daemon Thu Apr 4 15:04:39 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Thu, 4 Apr 2002 15:57:10 -0500
Subject: Manual for Polaron E6100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Neuberger,

We will have a copy of the E6100 manual in the mail to you tomorrow. As the
U.S. distributor for the Polaron Range of equipment, we have, or have access
to, manuals for almost all of the equipment they have offered throughout the
years. As a service to our customers, we are happy to supply those manuals
free of charge.

Please let us know if there is anything else you need. In the event that
you need service on the unit, we can provide that as well.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
www.ebsciences.com
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: Damian Neuberger [mailto:neuberger1234-at-attbi.com]
Sent: Wednesday, April 03, 2002 9:53 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone help me. I'm looking for a manual or copy of a manual for a
Polaron E6100 vacuum evaporator/sputter coater. It uses the E5350 Sputter
controller. I would be happy to pay for copying expense or if someone can
direct me to a vendor who may be able to provide the instruction manual we
would be willing to buy one.

Damian Neuberger, PhD
Department of Applied Sciences
Baxter Healthcare Corp.
P.O. Box 490
Rt. 120 & Wilson Rd
Round Lake, IL 60073
tel: 847.270.5888
fax: 847.270.5897
damian_neuberger-at-baxter.com






From daemon Thu Apr 4 23:56:11 2002



From: Kit Foo :      kfoo-at-uci.edu
Date: Thu, 4 Apr 2002 21:52:36 -0800
Subject: Digital Darkroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Everyone,

Is anyone familiar with the UMAX Powerlook 3000? We are looking to get a
scanner with both scanning reflective and transparent originals capability.
Agfa Duoscan T2500 and Duoscan Hi-D (or their mechanical twins Mircotek
Artixscan 2500 and Artixscan 1100) are some of the popular models, but the
technical specifications for the UMAX looks impressive too.

Thanks,

Kit



From daemon Fri Apr 5 01:33:26 2002



From: =?iso-8859-1?Q?Krzysztof_Jan_H=FCbner?= :      hubner-at-IOd.krakow.pl
Date: Fri, 5 Apr 2002 08:04:52 +0200
Subject: Re: answer LM - magnetic etching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


good morning

The magnetc etching technology you can find in the ASTM Handbook vol 9
Metalography and Microstructure, 1995 edition page 63 - 66 author R.J.
ray - Mangetic etching

best regards

Krzysztof Jan Hübner

Foundry Research Institute
30-418 Kraków, Poland


} I have seen references about magnetic etching of ferrite-austenitic
} stainless steels with a colloidal suspension of Fe(subscript: 3)O
} (subscript: 4). Do any of you have experience of this technique? From where
} can the etchant be purchased?

} Agneta Östberg
} AB Sandvik Steel
} SE-811 81 Sandviken
} Sweden

} phone: +46 26 264311
} fax: +46 26 264310
} e-mail: agneta.ostberg-at-sandvik.com





From daemon Fri Apr 5 01:42:07 2002



From: =?iso-8859-1?Q?Krzysztof_Jan_H=FCbner?= :      hubner-at-IOd.krakow.pl
Date: Fri, 5 Apr 2002 08:15:06 +0200
Subject: Re: LM - magnetic etching- 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I forget about book GF Vander Voort - Metallography principles and practice
McGraw-Hill1984, page 190-191 and 239-241

best regards

krzysztof jan hübner



From daemon Fri Apr 5 05:45:48 2002



From: Tom Schamp :      cts2v-at-virginia.edu
Date: Fri, 05 Apr 2002 06:36:06 -0600
Subject: Re: Digital Darkroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kit,
We have a UMAX Powerlook 2000 in our group and another lab near us has an
older Agfa. Both have transparency adapters to transmit light through the
transparency/negative. In their Agfa, the bottom glass and the glass from the
transparency adapter are in contact so there are only glass/film and film/glass
interfaces. In ours, there is a gap between the bottom and adapter glass to
permit negative holders. Therefore in ours, there is a glass/film interface, a
film/air interface, and finally an air/glass interface.
When I scan negatives of an SAD pattern with both scanners and then take an
intensity line trace across the SAD pattern, I find our UMAX to be much noisier
than their Agfa. I think this is due to the extra interfaces refracting the
light.
I have heard that the Microtek scanner with the drawer is nice because
there is no glass, but it only holds up to four negatives at a time. Since it
holds the negatives, some of the negative area gets cut off on the edges where
it is in contact with the holder drawer.

That is what I have found.

Tom Schamp

Kit Foo wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, Everyone,
}
} Is anyone familiar with the UMAX Powerlook 3000? We are looking to get a
} scanner with both scanning reflective and transparent originals capability.
} Agfa Duoscan T2500 and Duoscan Hi-D (or their mechanical twins Mircotek
} Artixscan 2500 and Artixscan 1100) are some of the popular models, but the
} technical specifications for the UMAX looks impressive too.
}
} Thanks,
}
} Kit



From daemon Fri Apr 5 08:56:32 2002



From: Karen L. Glyde :      klg2-at-psu.edu
Date: Fri, 05 Apr 2002 09:41:33 -0500
Subject: paraffin sectioning of drosophila heads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Goal: 5 um serial sections of drosophila heads from proboscis to skull "cap".

Procedure: Many of the heads fall out of the paraffin while sectioning
which suggests an infiltration problem. The researcher is doing the
processing using Carnoy's fixative with subsequent baths
of alcohol, methylbenzoate, Then we pick up and do the baths of 1:1
methylbenzoate/alcohol, four fresh paraffin baths and
then the embedding described above.

Heads are mounted in fly collars with the
proboscis resting on the collar..
I embed them by inverting the collar in an aluminum weigh
boat to obtain as flat a service as possible.
I remove the partially set paraffin with the row of fly
heads in a strip 3/16"wide and 1/8"deep which is the maximum amount I
can remove because of the design of the collar. I then
mount it on a paraffin blank.

Problems: I must remove the strip of paraffin with the fly heads while
the paraffin is still slightly malleable which means that it curls a bit.
Time is a critical factor here. If I wait until the
paraffin is harder the strip cracks as I remove it and the heads are lost. But
because it curls that little bit they are not flat enough
to section all the heads on the same plane.

Many of the heads fall out of the paraffin while
sectioning which suggests an infiltration problem.

Suggetions from those of you who have done fly paraffin work would be
greatly appreciated..


Karen L.Glyde
Electron Microscope Facility of the Lifes Sciences Consortium
Pennsylvania State University









From daemon Fri Apr 5 10:02:58 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Fri, 05 Apr 2002 10:55:23 -0500
Subject: Re: Petri Dishes with Gridded Coverslip Bottoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey David,
Check out MatTek 1-800-834-9018. I know they sell glasss bottom dishes
(#P35G-0-14C), so a grided one is not unreasonable. If not
you can always carbon coat a few EM grids onto the surface before growing
the cells, like we did in the old days. Good Luck.

Your old bud,
Mike Delannoy

David Spector wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
}
} I need to repeatedly image cells growing on a coverslip in medium
} using an inverted fluorescence microscope. Does anyone know of 35 mm
} Petri dishes that have a gridded coverslip as their bottom or of
} plastic dishes that don't autofluoresce?
}
} Thanks,
}
} David Spector
} --
} Dr. David L. Spector
} Cold Spring Harbor Laboratory
} One Bungtown Road
} Cold Spring Harbor, New York 11724
} Tel. (516) 367-8456
} Fax (516) 367-8876



From daemon Fri Apr 5 10:11:50 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Fri, 05 Apr 2002 11:07:24 -0500
Subject: Re: Sample Preparation for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chad,
It sounds like you must omit the transition step and substitute ETOH:EPON parts over long infiltration steps. When you get to pure Epon
I would then add the catalyst and try some R.T. vacuum infiltration. Good Luck.

Mike Delannoy

P.S. If you want to see membranes, don't forget the osmium.

Chad Friece wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have a question regarding sample preparation for Transmission electron microscopy. I am growing cells on a polystyrene surface which is in an enclosed environment. In this environment, the cells are fixed with gluteraldehyde. Following fixation, the polystyrene is sectioned or physically cut with a scalpel or a pair of scissors (with the fixed cells attached) and placed in buffer. The sections are dehydrated in increasing concentrations of EtOH. Next, the sections are put through a transition step in which they are exposed to 2-hydroxypropylmethacrylate, followed by embedding in an Epon derivative. My problem is somewhere between the transition step and the embedding step in which the polystyrene is actually being dissolved. Is there a method or reagent that can be used to prepare cells grown on polystyrene for TEM? I believe it would have to be some sort of water-based reagent/method because almost every organic solvent I have used has dissolved polystyrene. It has been sug!
} gested that I change the substrate for which the cells are grown; however, it is crucial that I use this polystyrene growth surface.
}
} Any suggestions would be greatly appreciated.
}
} Thank You,
}
} Chad Friece
} cfirece-at-biocrystal.com



From daemon Fri Apr 5 10:17:59 2002



From: Helvey, Marc :      Marc.Helvey-at-vlsistd.com
Date: Fri, 5 Apr 2002 08:12:27 -0800
Subject: About III-V Compound Semiconductor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This it?

http://www.three-fives.com/

Marc Helvey
Strategic Accounts Manager
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
Phone: (408) 428-1800, ext. 108
Internet: http://www.vlsistandards.com




-----Original Message-----
} From: Xianglin Li [mailto:Xianglin_Li-at-student.uml.edu]
Sent: Thursday, April 04, 2002 12:31 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

I heard that there is a free journal about III-V Compound
Semiconductor. Could anybody give me some information about this?

Thank you very much!

Xianglin

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Fri Apr 5 16:16:31 2002



From: Microscopy Today :      microtoday-at-attglobal.net
Date: Fri, 5 Apr 2002 17:02:54 -0500
Subject: MICROSCOPY TODAY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Today has a new owner and editor. Don Grimes started Microscopy
Today and has run the operation for ten years and made many friends in the
microscopy community. He has finally decided to retire.

About one year ago, Don began negotiations with MSA, the Microscopy Society
of America, to sell Microscopy Today to MSA. These negotiations are now
concluded. MSA now owns Microscopy Today and I have accepted the position of
Editor. I hope I can fill Don's shoes!

I have been an MSA member for over 35 years and have been both a user of
microscopy equipment and a vendor. It has been my privilege to serve the
society in various officer roles, culminating as MSA President in 2001. I
resigned my year on MSA Council as Past President prior to the finalization
of the negotiations.

I can assure you that I have enough sense to leave well-enough alone and I
plan to keep Microscopy Today pretty much the way it has been in recent
years--with perhaps a greater emphasis on all types of microscopy--not just
electron microscopy. The entire MSA membership will receive Microscopy Today
-- but MSA membership will not be a requirement for receiving Microscopy
Today. No subscribers will be dropped and we will continue to welcome anyone
with an interest in microscopy. MT will be published six times per year, in
odd months, interleaving with MSA's journal Microscopy and Microanalysis,
which publishes in even months and goes to MSA members and its subscribers.

Don Grimes will still be involved in MT as an advisor and consultant. Phil
Oshel has consented to continue in his role as article solicitor for the
Microscopy 101 section of MT, up to the time of the M&M Annual Meeting in
Quebec. We hope to bring both Don and Phil to Quebec to meet old friends
and to help out at the MT booth. There will be a "Just for Fun Micrograph
Contest" in Quebec. There will always be a place for humor in MT!

I am also 100% aware that Microscopy Today would not exist without the
support of its many readers, contributors and advertisers over the years. I
hope I can continue to work with all concerned to continue this service to
the microscopy community. I would just love to have more Microscopy Today
style articles, etc.(hint hint)

New Microscopy Today contact information is as follows:

Address: P.O. Box 499, Wappingers Falls, NY 12590
Courier: 21 Westview Drive, Poughkeepsie, NY 12603
Phone: 845 463-4124
FAX: 845 463-4125
E-mail: microtoday-at-attglobal.net

Sincerely yours,

Ron Anderson,
Editor, Microscopy Today



From daemon Fri Apr 5 18:29:44 2002



From: Re Nate :      renate10000-at-hotmail.com (by way of MicroscopyListserver)
Date: Fri, 5 Apr 2002 18:19:34 -0600
Subject: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

I tried to locate an ISO or NIST traceable magnification calibration
standard for TEM. But all the people tell me these traceable TEM
standards are not available. Can anyone explain me (in a simple way)
why these standards don’t exist.

Thanks Renate


_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp.


From daemon Fri Apr 5 21:48:51 2002



From: Bob McPherson :      bobmcp-at-linuxmail.org
Date: Sat, 06 Apr 2002 11:40:30 +0800
Subject: RE: Automated image analysis for particle size from SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--

Get your free email from www.linuxmail.org


Powered by Outblaze


From daemon Sat Apr 6 09:16:31 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Sat, 6 Apr 2002 10:06:25 -0500
Subject: Re: Sample Preparation for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


why polystyrene? use polypropylene the resists most organic solvents.


From daemon Sat Apr 6 11:06:37 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Sat, 6 Apr 2002 11:02:13 -0600
Subject: Polaron E6100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all,

I want to thank all of you who responded to my request for a manual for a
Polaron E6100 that I just inherited. I especially want to thank Michael R.
Nesta Energy Beam Sciences for sending me a manual and a referral to their
engineering manager for advice on what to do before starting in up after 15+
years of no use.

Damian Neuberger
Baxter Healthcare Corp.

I have no financial interest in Energy Beam Sciences just a user who has
just gotten great customer support.




From daemon Sat Apr 6 14:04:42 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Sat, 06 Apr 2002 11:51:01 -0800
Subject: Re: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Renate:

We have been offering for several years the MAG*I*CAL TEM calibration
standard
and have been working with NIST trying to get it certified. The main
problem
seems to be money. If we can get enough people to contact NIST
requesting such
a certified standard, then they may find the budget to make to happen.

I will be happy to take the lead on getting this through. If anyone out
there has a desire to have a certified TEM calibration standard, please
email your request to me. I will compile them and present them to NIST
with a formal request. You can make it simple one line request or feel
free to go into detail about why such a standard is needed.

In the interim, I wouold be pleased to send to anyone information on the
MAG*I*CAL and our reasoning on why it should be readily accepted as a
certifiable standard. Our explanation has satisfied many users for their
internal purposes, but we would certainly like to see a NIST traceable
"certification".

I'll wait to hear from you!

DISCLAIMER: South Bay Technology produces equipment and supplies as
described
above and, therefore, has a vested interest in promoting their use.

Best regards-

David


"Re Nate (by way of MicroscopyListserver)" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Listers,
}
} I tried to locate an ISO or NIST traceable magnification calibration
} standard for TEM. But all the people tell me these traceable TEM
} standards are not available. Can anyone explain me (in a simple way)
} why these standards don’t exist.
}
} Thanks Renate
}
} _________________________________________________________________
} Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp.

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Sat Apr 6 14:31:40 2002



From: Blackwood, Andrew :      ablackwood-at-2spi.com
Date: Sat, 06 Apr 2002 15:19:07 -0500
Subject: Calibration Standard Traceability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Blackwood, Andrew * EMC.Ver #3.1a ] --

6 April 2002

Hi Listers:

Renate asked what would appear to be a very simple question about the
availability of traceable standards for TEM. There really are a couple of
valid reasons underlying the problem, and there is a solution.

Actually, the rules of the game, which are based on ISO 17025, the
international standard for laboratory accreditation, require that
measurements be traceable to either a national standards laboratory (NIST,
in the U.S.) or to a fundamental natural constant. The American Association
for Laboratory Accreditation adds the wrinkle that the traceability must be
through an unbroken chain of accredited laboratories. That's the basis for
the problem; there are no laboratories accredited to make the necessary
measurements in the U.S.

Now, the rules allow you to do any sort of calibration within your own
operation, provided that you establish traceability. So if you have
something that is NIST traceable or traceable to a fundamental natural
constant, you can trace your internal measurements to whatever that is. NIST
has offered microspheres, although a search of their website a couple of
minutes ago couldn't come up with a SRM (standard reference material) number
. A number of vendors, including SPI Supplies, offer a range of microspheres
which are sized based on those NIST samples.

The Mag*I*Cal specimen (SPI #02218-AB), however, bypasses the whole question
of accreditation by being based on the fundamental properties of the element
silicon. As a result, you can bypass NIST completely. More information on
this sample can be found at

http://www.2spi.com/catalog/standards/magical.html

A note about NIST: I have nothing but the highest respect for the microscopy
group at NIST. I don't know what their plans are, but I suspect that they
are caught between a rock (the high requirements placed on standard
reference materials) and a hard place (the realities of the world of
microscopy). The kinds of uncertainty that we routinely accept in our work
are simply not acceptable in the world of metrology (the art and science of
measurement). I also suspect that their priorities are directed in
directions other than TEM magnification.

Disclaimer: I am laboratory director of Structure Probe, Inc., an
independent analytical research laboratory which offers electron microscopy
services to clients. Structure Probe, Inc. is the parent company of SPI
Supplies. We have an obvious interest in promoting microscopy in general and
the use of the products we sell in particular. Structure Probe has been
involved with the American Association for Laboratory Accreditation since it
was founded in the 1970s. For the last several years, I have served as an
assessor, a member of the Accreditation Council (the group that makes the
actual decision whether a laboratory is or is not granted accreditation) and
a member of the Measurement Advisory Committee (the group that considers the
specific problems of metrology laboratories) for A2LA.

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
P.O. Box 656
West Chester, PA 19381-0656
Ph: 1 610 436 5400 X108
FAX: 1 610 436 5755
e-mail: ablackwood-at-2spi.com
WWW: http://www.2spi.com




From daemon Sat Apr 6 15:46:01 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Sat, 06 Apr 2002 16:38:24 -0500
Subject: Re: paraffin sectioning of drosophila heads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a fromer position I had a researcher attempting the same thing. He eventually went to the cryostat and also used LR-White embedded sections. Cryostat was his eventual choice.

} } } "Karen L. Glyde" {klg2-at-psu.edu} 04/05/02 18:33 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Goal: 5 um serial sections of drosophila heads from proboscis to skull "cap".

Procedure: Many of the heads fall out of the paraffin while sectioning
which suggests an infiltration problem. The researcher is doing the
processing using Carnoy's fixative with subsequent baths
of alcohol, methylbenzoate, Then we pick up and do the baths of 1:1
methylbenzoate/alcohol, four fresh paraffin baths and
then the embedding described above.

Heads are mounted in fly collars with the
proboscis resting on the collar..
I embed them by inverting the collar in an aluminum weigh
boat to obtain as flat a service as possible.
I remove the partially set paraffin with the row of fly
heads in a strip 3/16"wide and 1/8"deep which is the maximum amount I
can remove because of the design of the collar. I then
mount it on a paraffin blank.

Problems: I must remove the strip of paraffin with the fly heads while
the paraffin is still slightly malleable which means that it curls a bit.
Time is a critial factor here. If I wait until the
paraffin is harder the strip cracks as I remove it and the heads are lost. But
because it curls that little bit they are not flat enough
to section all the heads on the same plane.

Many of the heads fall out of the paraffin while
sectioning which suggests an infiltration problem.

Suggetions from those of you who have done fly paraffin work would be
greatly appreciated..


Karen L.Glyde
Electron Microscope Facility of the Lifes Sciences Consortium
Pennsylvania State University











From daemon Sun Apr 7 01:41:48 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Sat, 06 Apr 2002 23:21:03 -0800
Subject: Re: Calibration Standard Traceability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Andy:

You explained the situation very concisely and with much greater clarity than I
ever could! Traceability to a fundamental natural constant is exactly what we
would like NIST to certify.

With regard to the traceability and certification of the MAG*I*CAL™
calibration sample, each sample is grown on {001} oriented single
crystal silicon, and all spacings on the sample are directly referenced
to the cross-sectional (111) lattice spacing of silicon. This spacing
is visible by lattice imaging on the sample itself, giving each sample
the capability of being self-calibrating. Each unit comes with a
numbered certificate, the text of which is included below. This
certificate has been used for ISO 9000 certification, with the argument
that to our knowledge, this is the highest quality TEM sample available
anywhere in the world at this time.

The MAG*I*CAL ™ calibration sample consists of sets of thin, nominally
10 nm alloy layers of Si0.81Ge0.19 alternating with 10 nm pure silicon
layers, on a single crystal silicon {001} substrate. These electronic
device quality layers were grown by Molecular Beam Epitaxy (MBE) as
strained layers, i.e., the alloy layers have a slightly different
crystal lattice constant, but are strained to conform to the lattice
spacing of pure silicon, so that the material remains single crystal.
Lattice images should therefore be taken in the region of the sample
containing no Ge, but other measurements are unaffected. The layer
thickness variation across the wafer was measured by double crystal
x-ray diffraction (DCXRD) mapping as { 1.0%.

All four sets of the five thin Si0.81Ge0.19 alloy layers and alternating
pure silicon layers (superlattices) were directly calibrated by high
resolution transmission electron microscopy (HREM) with the
cross-sectional (111) lattice spacing of the single crystal silicon
substrate, equal to 0.313543 nm [1]. These measurements are also
supported by (DCXRD).

The error in all spacings in the superlattices is one atomic layer:

¦t = +0.3 nm or approximately +3%

The larger, nominally 1.0 micron silicon spacings were calibrated
against these superlattices. The total error across the entire
calibration sample is given as:

¦t = + 3%

[1] CRC Handbook of Chemistry and Physics, CRC Press, Inc., Boca
Raton, Florida 33431

This explanantion has been sufficient for many of our customers when certifying
their calibration method. I hope it helps.

DISCLAIMER: While available through various suppliers, South Bay
Technology is the exclusive, worldwide master distributor of the
MAG*I*CAL™ and, therefore, certainly has a vested interest in promoting
its use.

Best regards-

David

"Blackwood, Andrew" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} -- [ From: Blackwood, Andrew * EMC.Ver #3.1a ] --
}
} 6 April 2002
}
} Hi Listers:
}
} Renate asked what would appear to be a very simple question about the
} availability of traceable standards for TEM. There really are a couple of
} valid reasons underlying the problem, and there is a solution.
}
} Actually, the rules of the game, which are based on ISO 17025, the
} international standard for laboratory accreditation, require that
} measurements be traceable to either a national standards laboratory (NIST,
} in the U.S.) or to a fundamental natural constant. The American Association
} for Laboratory Accreditation adds the wrinkle that the traceability must be
} through an unbroken chain of accredited laboratories. That's the basis for
} the problem; there are no laboratories accredited to make the necessary
} measurements in the U.S.
}
} Now, the rules allow you to do any sort of calibration within your own
} operation, provided that you establish traceability. So if you have
} something that is NIST traceable or traceable to a fundamental natural
} constant, you can trace your internal measurements to whatever that is. NIST
} has offered microspheres, although a search of their website a couple of
} minutes ago couldn't come up with a SRM (standard reference material) number
} . A number of vendors, including SPI Supplies, offer a range of microspheres
} which are sized based on those NIST samples.
}
} The Mag*I*Cal specimen (SPI #02218-AB), however, bypasses the whole question
} of accreditation by being based on the fundamental properties of the element
} silicon. As a result, you can bypass NIST completely. More information on
} this sample can be found at
}
} http://www.2spi.com/catalog/standards/magical.html
}
} A note about NIST: I have nothing but the highest respect for the microscopy
} group at NIST. I don't know what their plans are, but I suspect that they
} are caught between a rock (the high requirements placed on standard
} reference materials) and a hard place (the realities of the world of
} microscopy). The kinds of uncertainty that we routinely accept in our work
} are simply not acceptable in the world of metrology (the art and science of
} measurement). I also suspect that their priorities are directed in
} directions other than TEM magnification.
}
} Disclaimer: I am laboratory director of Structure Probe, Inc., an
} independent analytical research laboratory which offers electron microscopy
} services to clients. Structure Probe, Inc. is the parent company of SPI
} Supplies. We have an obvious interest in promoting microscopy in general and
} the use of the products we sell in particular. Structure Probe has been
} involved with the American Association for Laboratory Accreditation since it
} was founded in the 1970s. For the last several years, I have served as an
} assessor, a member of the Accreditation Council (the group that makes the
} actual decision whether a laboratory is or is not granted accreditation) and
} a member of the Measurement Advisory Committee (the group that considers the
} specific problems of metrology laboratories) for A2LA.
}
} Andy
}
} Andrew W. Blackwood, Ph.D.
} Structure Probe, Inc.
} P.O. Box 656
} West Chester, PA 19381-0656
} Ph: 1 610 436 5400 X108
} FAX: 1 610 436 5755
} e-mail: ablackwood-at-2spi.com
} WWW: http://www.2spi.com

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Sun Apr 7 21:04:30 2002



From: Hao Cheng :      haohao-at-glay.org
Date: Mon, 08 Apr 2002 09:50:59 +0800
Subject: RE: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--

Get your free email from http://www.glay.org

Powered by Outblaze


From daemon Mon Apr 8 07:39:14 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Mon, 8 Apr 2002 08:25:20 -0400
Subject: Re: Petri Dishes with Gridded Coverslip Bottoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


try EMS they have a grided coverslip. sorry if you needed more specific info
on just petrie dishes, i missed your original post.
john


From daemon Mon Apr 8 15:46:20 2002



From: Sophie Dahan :      sdahan-at-caprion.com
Date: Mon, 8 Apr 2002 16:36:22 -0400
Subject: Tissue Array Slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I was wondering if anyone knows where we can purchase slides of paraffin-embedded and/or frozen tissue arrays.

Thanks,

Sophie Dahan, Ph.D.
Senior Scientist, Microscopy Lab
Caprion Pharmaceuticals, Inc.
Montreal, Quebec
Canada



From daemon Mon Apr 8 23:23:32 2002



From: Er Poh Nee (Yu Baoni) :      nmierpn-at-nus.edu.sg
Date: Tue, 9 Apr 2002 12:13:52 +0800
Subject: Imagining of Plakton

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

We have lots of difficulties in trying to image unicellular plankton
(flagellates)

Our aim is to obtain as accurately as possible the surface area volume of
such organism.

We have tired to do optical sectioning using
1) autofluorescence
2) stain with acridine orange
but the signal usually do not represent ( or stain) the entire volume.

3) transmitted mode
but this is difficult to define the upper and lower limit of the organism.

Do anyone have any good suggestions/ experiences? What are the suitable dyes
to use?

LiJia and other


From daemon Tue Apr 9 08:08:02 2002



From: zaluzec-at-microscopy.com
Date: Tue, 9 Apr 2002 07:51:05 -0500
Subject: Administrivia: March Archives are now on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....


The Listserver archives are now up todate through March 31, 2002

Nestor
Your Friendly Neighborhood SysOp.


From daemon Tue Apr 9 08:14:20 2002



From: =?iso-8859-1?Q?=22Isabelle_C=F4t=E9=22?= :      i_cote-at-makivik.org (by way of
Date: Tue, 9 Apr 2002 08:05:47 -0500
Subject: manual tissue processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking to set up small-scale histology facilities using manual
tissue processing (solely due to the limited budget). Can anyone recommend
me a company which could provide us with 2 wax bath one of them having
vacuum capabilities.

thank you for your help
Isabelle Cote
Nunavik Research Centre


From daemon Tue Apr 9 08:37:10 2002



From: CHEN CHEN :      cchen5-at-jhmi.edu
Date: Tue, 09 Apr 2002 09:33:59 -0400
Subject: Please subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please subscribe.
Thanks

Chen Chen
Department of Biological Chemistry
The Johns Hopkins University School of Medcine
725 N. Wolfe Street
Baltimore, MD 21205



From daemon Tue Apr 9 08:57:48 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 9 Apr 2002 09:51:34 -0400
Subject: MICROSCOPY TODAY

Contents Retrieved from Microscopy Listserver Archives
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I would like to LOUDLY APPLAUD Don for a job well done. And I would also like to thank Phil (although I still owe him several articles) for his role in making MT a success. Well done, Gentlemen.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Microscopy Today [mailto:microtoday-at-attglobal.net]
Sent: Friday, April 05, 2002 5:03 PM
To: microscopy-at-sparc5.microscopy.com


Microscopy Today has a new owner and editor. Don Grimes started Microscopy
Today and has run the operation for ten years and made many friends in the
microscopy community. He has finally decided to retire.

About one year ago, Don began negotiations with MSA, the Microscopy Society
of America, to sell Microscopy Today to MSA. These negotiations are now
concluded. MSA now owns Microscopy Today and I have accepted the position of
Editor. I hope I can fill Don's shoes!

I have been an MSA member for over 35 years and have been both a user of
microscopy equipment and a vendor. It has been my privilege to serve the
society in various officer roles, culminating as MSA President in 2001. I
resigned my year on MSA Council as Past President prior to the finalization
of the negotiations.

I can assure you that I have enough sense to leave well-enough alone and I
plan to keep Microscopy Today pretty much the way it has been in recent
years--with perhaps a greater emphasis on all types of microscopy--not just
electron microscopy. The entire MSA membership will receive Microscopy Today
-- but MSA membership will not be a requirement for receiving Microscopy
Today. No subscribers will be dropped and we will continue to welcome anyone
with an interest in microscopy. MT will be published six times per year, in
odd months, interleaving with MSA's journal Microscopy and Microanalysis,
which publishes in even months and goes to MSA members and its subscribers.

Don Grimes will still be involved in MT as an advisor and consultant. Phil
Oshel has consented to continue in his role as article solicitor for the
Microscopy 101 section of MT, up to the time of the M&M Annual Meeting in
Quebec. We hope to bring both Don and Phil to Quebec to meet old friends
and to help out at the MT booth. There will be a "Just for Fun Micrograph
Contest" in Quebec. There will always be a place for humor in MT!

I am also 100% aware that Microscopy Today would not exist without the
support of its many readers, contributors and advertisers over the years. I
hope I can continue to work with all concerned to continue this service to
the microscopy community. I would just love to have more Microscopy Today
style articles, etc.(hint hint)

New Microscopy Today contact information is as follows:

Address: P.O. Box 499, Wappingers Falls, NY 12590
Courier: 21 Westview Drive, Poughkeepsie, NY 12603
Phone: 845 463-4124
FAX: 845 463-4125
E-mail: microtoday-at-attglobal.net

Sincerely yours,

Ron Anderson,
Editor, Microscopy Today



From daemon Tue Apr 9 09:54:51 2002



From: Allan J. MacKenzie :      al.mackenzie-at-lakeheadu.ca
Date: Tue, 9 Apr 2002 10:47:47 -0400
Subject: SEM-Photoshop Help on Background Correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Group,

We have recently switched from traditional darkroom processing of SEM images
to the digital world and are starting to use Photoshop 6.0 for final image
processing before publication. We are a long way from being experts in
Photoshop and are not quite sure how to set the background to black while
maintaining good tonal range in a centred object such as an insect part.
Have tried the PS 6.0 magic wand tool to mark off the background and it
seems to work ok except for samples with fine details protruding from the
object.

Suggestions?

Thanks,

Allan J.MacKenzie
Lakehead University



From daemon Tue Apr 9 10:10:18 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Tue, 9 Apr 2002 11:03:39 -0400 (EDT)
Subject: Long-term storage, CPD'd samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all...

Is anyone personally familiar with long-term storage of
of critical point dried specimens (or aware of any publications on the
subject)? We are going to being adding some dried
specimens to our collections (stored in properly dessicated chambers) and
I am interested to know what the maximum "shelf-life" is for such
specimens. For us, long-term means forever (or as close as possible).

Many thanks in advance.

Best,

Angela
-----------------------------------------
**Please note new department name**

Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------



From daemon Tue Apr 9 10:49:09 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 9 Apr 2002 08:42:39 -0700 (PDT)
Subject: Re: SEM-Photoshop Help on Background Correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Allan,

A simple way to set your background is: Image} Adjust} Levels. In the Levels
window you will see three color dropper boxes in a row. Click on the one
on the left and then click on the region of your image that you want to be
the darkest shadow area and it will set your shadow value in the image.
The dropper box on the right can be used to set your highlight value. The
middle dropper works on color images to set a color balance if you click
on an area that should be a neutral grey, it will do a color balance based
on that selection.

Bob
U of Washington
Seattle

On Tue, 9 Apr 2002, Allan J. MacKenzie wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Group,
}
} We have recently switched from traditional darkroom processing of SEM images
} to the digital world and are starting to use Photoshop 6.0 for final image
} processing before publication. We are a long way from being experts in
} Photoshop and are not quite sure how to set the background to black while
} maintaining good tonal range in a centred object such as an insect part.
} Have tried the PS 6.0 magic wand tool to mark off the background and it
} seems to work ok except for samples with fine details protruding from the
} object.
}
} Suggestions?
}
} Thanks,
}
} Allan J.MacKenzie
} Lakehead University
}
}
}



From daemon Tue Apr 9 15:42:37 2002



From: Haifeng.Wang-at-ReadRite.com
Date: Tue, 9 Apr 2002 13:35:29 -0700
Subject: Electron Microscopy Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A engineer or senior technician position is available at Read-Rite Corp with
focus on electron microscopy.
For details, please see below.


Company: Read-Rite Corporation. Read-Rite Corporation is one of the world's
leading
independent manufacturers of magnetic recording heads, head gimbal
assemblies (HGAs)
and head stack assemblies (HSAs) for disk drives and tape drives. The
company is
headquartered in Fremont, California and has operations in California,
Thailand, the
Philippines, Japan, Singapore and South Korea. The company's website is
located at
http://www.readrite.com.

Department: Materials Development

Location: Fremont, California

Qualifications: A.S. or B.S. degree in physical science, materials science
and
engineering,

Responsibilities: Engineer position or senior technician is available for
structural and
chemical characterization and analysis of magnetic recording heads in the
rapidly
changing production and new product development environment. Main
responsibilities
include operation and routine maintenance of JEOL 2011F TEM with Gatan CCD
camera, Gatan Imaging Filter, and EDAX X-ray detector, as well as the
attached
computer systems for image processing and archive. The selected candidate
is also
responsible for generating TEM images and spectra in publishable quality to
feedback on
production and development. In addition, the candidate is closely involved
in sample
preparation using dual beam FIB, mechanical lapping, and ion milling in the
team-
working environment. Other responsibilities include interaction with
electron
microscope vendors, working with service engineers, and service records
maintenance.
The successful candidate will be exposed to internal and external
multifunctional groups
to provide problem-solving plans.

Required skills: This position requires a bachelor or associate degree in
materials science
and engineering and/or physics, with focused experience in electron
microscopy. Must
have extensive academic and/or at least 3 years of industrial experience. A
strong
background and extensive experience in TEM, SEM, FIB, ion milling, wedge
polishing,
and vacuum technology are essential. Experience with dark room photography
and
digital imaging techniques with a broad experience in image processing,
archiving, and
networking on PC/Mac platforms is desired. The preferred candidate is
strongly self-
motivated, innovative and creative in developing and improving existing
imaging and
analytical techniques. Strong interpersonal, verbal and written
communication skills are
a plus.

Please respond to the job off-line with me at Haifeng.wang-at-readrite.com.





From daemon Tue Apr 9 16:53:58 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Tue, 9 Apr 2002 16:31:16 -0500
Subject: Immuno-labeling polymerizing beta keratin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listies,

We are interested in imaging the polymerization of beta keratin in
developing pin feathers with immunolabeling.

Does anyone have experience labeling keratin?

Does keratin autofluoresce?

In birds we trust,
Tim Quinn
University of Kansas
Program Assistant
Ornithology Dept.
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu




From daemon Tue Apr 9 20:22:43 2002



From: John Winter :      winterj-at-whitman.edu (by way of MicroscopyListserver)
Date: Tue, 9 Apr 2002 20:11:28 -0500
Subject: Backscatter detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have some funds for a new high-res BSE detector for our SEM (a JEOL
JSM T-300) so I can finally find my way around a polished rock thin
section on the basis of mean atomic number of the minerals. I know
that JEOL, ETP-Robinson, and GW Electronics make them. Does anybody
have a recommendation as to which is the best buy? Have I missed
somebody? Thanks.


Dr. John D Winter
Department of Geology
Whitman College
345 Boyer Ave
Walla Walla, WA 99362 USA
(509) 527-5113
Fax: (509) 527-5904
e-mail: winterj-at-whitman.edu
web page:
{http://www.whitman.edu/offices_departments/geology/winter/} http://www.whitman.edu/offices_departments/geology/winter/


From daemon Tue Apr 9 20:50:20 2002



From: eduwinfuentes6678-at-carmsa.co.za
Date: Mon, 08 Oct 2001 19:35:32 -1600
Subject: W h a t d o y o u R E A L L Y K n o w a b o u t y o u r P a r t n e r ? HEP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We Run FULL NATIONAL Asset & Background Searches.

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From daemon Tue Apr 9 23:05:20 2002



From: Edward_Principe-at-amat.com
Date: Tue, 9 Apr 2002 20:55:39 -0700
Subject: looking for kunli@lycosasia.com: Re TEM prep for 20A gate oxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kun Li,

I wanted to let you know that I tried to respond to your offline email
several times, but for some reason, it continues to fail. Perhaps that
email is no longer valid. On the chance you can recieve this message
through the listserver, I will respond in brief:

You have a good microscope in the Techni Stwin, congratulations. I assume
you have a 200kV system. Your estimate of delocalization I can't vouche
for, sounds a bit high? Dave Muller did a study on the interface
based upon the electronic structure transition (based upon the PEELS) and
found ~3A interphase on side. This basically indicates when a silicon atom
no longer sufficiently coordinated by oxygen to electronically behavior as
oxide.....see his papers for the proper interpretation.

Do you have the focal series software on your system? It is produced by
Philips (FEI now). This allows you to automatically acquire a
through-focus series of images. It is the first step in completing the
exit wave reconstruction to restore the phase infomation and remove the
delocalization from the image. If you can do this, then I can direct you
toward some help with the rest of the process. However, I believe the STEM
mode is still your best approach for consistent oxide thickness in this
case.
There is also the "autogate" routine for the STEM, which is at least a
consistent method (Muller again has his own publish approach).

I have attached the presentation referenced earlier. The paper is not yet
complete.

Regards,
Ed Principe, Ph.D.
Member of Technical Staff
Defect & Thin Film Characterization Laboratory
Applied Materials



From daemon Wed Apr 10 07:49:34 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 10 Apr 2002 08:44:01 -0400
Subject: About III-V Compound Semiconductor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Xianglin;

Here is a website that you or others may find interesting on 3-5 compound
semiconductors.

http://www.compoundsemiconductor.net/

Regards,
Peter Tomic
Anadigics, Inc.
Warren, New Jersey


-----Original Message-----
} From: Xianglin Li [mailto:Xianglin_Li-at-student.uml.edu]
Sent: Thursday, April 04, 2002 3:31 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

I heard that there is a free journal about III-V Compound
Semiconductor. Could anybody give me some information about this?

Thank you very much!

Xianglin

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Wed Apr 10 09:37:11 2002



From: Sophie Dahan :      sdahan-at-caprion.com
Date: Wed, 10 Apr 2002 10:24:01 -0400
Subject: Tissue Array Slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was told to post a reply on which companies sell tissue array slides of paraffin-embedded or frozen tissues.....here they are:

DAKO
Abcam
Ardais (very expensive - $1000/slide)

Sophie Dahan, Ph.D.
Senior Scientist, Microscopy Lab
Caprion Pharmaceuticals, Inc.
Montreal, Quebec
Canada



From daemon Wed Apr 10 11:06:15 2002



From: Bob Harris :      bharris-at-micro.uoguelph.ca
Date: Wed, 10 Apr 2002 11:57:06 -0400
Subject: Interpreting EELS spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All:

We have an EFTEM which we hope to use more efficiently to
interpret results of biogeochemical experiments. We are working
primarily with iron compounds and would like to be able to recognize
different valency states formed during incubation with microbes.
What I need is a course and/or fundamental reference material to
ease me into this. Is anyone aware of of something dealing with
EELS spectra that I could take advantage of somewhere in North
America?
On another note I would like to thank all those who responded to
my request for parts for my Zeiss photomicroscope. I have located
the needed parts in Florida and will soon have a complete LM. bob
Bob Harris
NSERC Regional STEM Facility
Dep't of Microbiology
University of Guelph
Guelph Ontario
Canada N1G 2W1
Phone: 519-824-4120 ext 6409
Fax: 519-837-1802


From daemon Wed Apr 10 11:20:00 2002



From: RCHIOVETTI-at-aol.com
Date: Wed, 10 Apr 2002 12:13:53 EDT
Subject: RNAse Free Section Adhesives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow List Members,

Vendors, please feel free to respond to this posting (as well as experienced
users), either directly to the list or off-list.

We are preparing some specimens (frozen sections) for laser microdissection
on thin polymer films which tend to be fairly hydrophobic. We've given some
thought to using an adhesive to increase the film's "stickiness" and improve
the adhesion of the frozen sections to the film.

The molecular biologists in the group don't have a problem with the use of
adhesives in the prep, *as long as* the adhesive is RNAse-free.

Does anyone out there know if there are certified RNAse-free adhesives that
would do the trick?

Many thanks!

Bob Chiovetti
GTI Microsystems
rchiovetti-at-aol.com


From daemon Wed Apr 10 11:21:03 2002



From: Lou bustillos :      lbustillos-at-amalab.com
Date: Wed, 10 Apr 2002 12:13:54 -0400
Subject: Microscopist Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a microscopist who has direct experience in analyzing
samples for asbestos using TEM, PLM and PCM. We have been in the
environmental field for about twenty years. The lab is located 5 minutes
away from Washington, DC.

Qualifications: A.S. or B.S. degree in physical science, material science
or in the life sciences.

Please email resume directly to me.

Luis H. Bustillos
AMA Analytical Services, Inc.
email: lbustillos-at-amalab.com
website: www.amalab.com




From daemon Wed Apr 10 12:42:15 2002



From: Peter West :      swlggymor-at-ayna.com
Date: Wed, 10 Apr 2002 12:38:46 -0500 (CDT)
Subject: I Thought You Would Like To Know dyazq

Contents Retrieved from Microscopy Listserver Archives
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From daemon Wed Apr 10 13:31:15 2002



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Wed, 10 Apr 2002 11:29:16 -0700
Subject: Re: Copper grid for lift-out of FIB prepaired samples on Gate Oxide

Contents Retrieved from Microscopy Listserver Archives
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Ed Principe wrote:
} But, that is not available for everyone...

On the other hand, my aim in designing and implementing the OÅM at the NCEM was to make it available for everyone. At least, everyone who would need the capability for sub-Angstrom microscopy (and could demonstrate such a need to the NCEM Steering Committee). Ed, I am happy to see that it worked for your project on gate oxides.
Mike O'Keefe

For more info on the NCEM One-Ångstrom Microscope project, see:
1. “The NCEM One-Ångstrom Microscope project reaches 0.89Å resolution”, M. A. O'Keefe in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000) 1192-1193.
2. “Sub-Ångstrom transmission electron microscopy at 300keV”, M.A. O’Keefe, E.C. Nelson, J.H. Turner and A. Thust in 59th Ann. Proc. MSA, Long Beach, California (2001) 898-899, invited.
3. "Sub-Ångstrom High-Resolution Transmission Electron Microscopy at 300keV”, M.A. O’Keefe, C.J.D. Hetherington, Y.C. Wang, E.C. Nelson, J.H. Turner, C. Kisielowski, J.-O. Malm, R. Mueller, J. Ringnalda, M. Pan and A. Thust. Ultramicroscopy 89 (2001) 4: 215-241.
4. “Sub-Ångstrom resolution of atomistic structures below 0.8Å”, M.A. O’Keefe, E.C. Nelson, Y.C. Wang and A. Thust, Philosophical Magazine B 81 (2001) 11: 1861-1878.

"Edward_Principe-at-amat.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Li,
}
} The quality of the final TEM sample, no matter what method is used to thin,
} depends most strongly on the final surface condition (roughness included).
} This means that the best TEM samples for a gate oxide application should
} have a final step that produces very thin cross sections of minimal
} roughness. FOR TEM, this should be less than ~60A if you don't want the
} dark band at the oxide/substrate interface caused by extinction
} oscillations. Depending upon your objective, you will need to be very
} cautious. FIB (pre-thinned or lift-out) will generally not achieve this
} objective alone so you need to follow on with low energy low angle ion
} milling or chemical thinning where applicable. Pre-thinned and lift-out
} FIB samples are not ideally suited for following with ion milling for
} super resolution work, but the company OmniProbe makes a micro-manipulater
} that goes into one of the gas injection ports and this allows you to "weld"
} the lift out specimen onto a holder inside the FIB (
} http://www.omniprobe.com/). Since there is no carbon/formvar grid (which
} you can't use for the best results, but a light carbon deposition on the
} bottom of the sample is needed), you can do post ion milling. I am also
} personally working on doing the final milling in-situ inside the SEM with a
} low energy ion argon gun (~250eV-500eV) on the free standing sample. If
} you are trying to obtain highest accuracy for absolute thickness using TEM
} on a 20A gate oxide there are several issues you will want to consider.
} You may require a TEM with better performance than you have available,
} again depending upon your objective. For absolute thickness and
} consistency, STEM is more forgiving of the sample preparation (see D.
} Muller's papers) and the methods to determine thickness can be applied in
} most cases more consistently than with TEM. The two techniques (TEM vs
} STEM) may also yield different results as STEM is more sensistive to both
} chemical contrast at the interface and physical roughness, often thought of
} as very similar but originating from either physical or chemical disorder
} at the interface. There are entirely separate problematic issues when
} working on high-K materials that can be hygroscopic (again see Muller).
}
} Some of these very issues you are struggling with have been the topic of
} some recent presentations by myself and others whom I have had the good
} fortune to work with, including world class TEM and STEM experts.
}
} This material was presented on 20A nitrided gate oxide analyzed by TEM/STEM
} and XPS at the recent AVS in San Francisco Oct28-Nov2 2001
} "Pushing the Limits of Nitrided Gate Oxide Materials: The Materials
} Characterization Role of TEM/STEM PEELS and XPS"
} E. Principe, A. Hegedus, C. Kisielowski, C. Song, B. Freitag, D. Hubert, T.
} Fliervoet, J. Gibson, J. Moulder, D. Watson
}
} I am also fortunate to collaborate on a paper with Christian Kisielowski
} (excellent TEM expert at National Center of Electron Microscopy in
} Berkeley), Alain Diebold, B. Foran (both of SEMATECH). David Muller (Bell
} Labs,Lucent), S. Pennycook (Oak Ridge), E. Principe (Applied Materials), S.
} Stemmer (Rice University) that is in preparation called "Thin Dielectric
} Film Thickness Determination by Advanced Transmission Electron Microscopy"
}
} The purpose of this paper is to address many of the issues to bear in mind
} in obtaining quantitative information on these very thin layers by TEM/STEM
} and PEELS, as well as XPS.
}
} So, you are not alone in your struggle and it is important to
} remember.......you will get "an answer" from whatever TEM sample you
} produce....but is it the correct answer for your purpose?? Paraphrasing
} John Henry Scott (NIST) as he put it in one of his lucid papers on the
} subject, the TEM images (interferograms really) are very impelling and thus
} you want to believe them. But, he showed you can have over 3.3A
} statistical error in the gate oxide measurement.....splitting hairs (or
} about 1/30,000th of a hair) you say, but when the gate is only 20A in the
} first place this is significant. Our work got the accuracy down to
} ~0.4-0.7A using focal series acquisition and exit wave reconstruction on
} the world's highest resolution TEM at NCEM (I believe it still has the
} record with ~80pm resolution). But, that is not available for everyone
} although Christian has taken it a long way toward become nearly routine. I
} believe I am safe to say he has done more of these FSR/ EWR than anyone
} else in the world.
}
} Good Luck!
}
} Ed Principe
} Member of Technical Staff
} Defect & Thin Film Characterization Laboratory
} Applied Materials



From daemon Wed Apr 10 15:54:43 2002



From: nickmoy-at-u.washington.edu
Date: Wed, 10 Apr 2002 13:46:28 -0700 (PDT)
Subject: LM-looking for a non-bleeding, non-permanent mounting media

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,
I'm looking for a mounting media that will work with Toluidine Blue stained turkey bone sections. I've tried using a gel mount, but the stain bleeds out after a day. I know there are plenty of mounting mediums out there that hold the stains, but i'm looking for one that will be non-permanent and not bleed at the same time. Any suggestions?!

Nicholas Moy
University of Washington
nickmoy-at-u.washington.edu






From daemon Wed Apr 10 18:09:06 2002



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Wed, 10 Apr 2002 18:04:02 -0500
Subject: EELS oxidation state techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Dr. Harris,
You might be interested in several of my papers on the study of Cr(VI)
reduction by Shewanella oneidensis. I measured the oxidation state of Cr
precipitates encrusting bacterial cells using EELS. I am presenting
analyzing the distribution of reduced forms of Cr(VI) within the cells.

Daulton T. L., Little B. J., Lowe K., and Jones-Meehan J. In-situ
Environmental Cell - Transmission Electron Microscopy Study of Microbial
Reduction of Chromium(VI) using Electron Energy Loss Spectroscopy,
Microscopy and Microanalysis 7, 470-485 (2001).

Daulton T. L., Little B. J., Lowe K., and Jones-Meehan J., Electron Energy
Loss Spectroscopy Techniques for the Study of Microbial Chromium(VI)
Reduction, Journal of Microbiological Methods, in press (2002).

The second paper is a techniques paper.

Iron is one of the more challenging metals to determine oxidation state
using EELS. It requires a spectrometer with a very good energy resolution
and good standards to base your identification upon. I am examining Fe
reduction by S. oneidensis as well.

A comprehensive source of information on EELS is naturally found in the
book by Egerton and, of course, Gatan Inc. offers EELS short courses.
I hope some of this information is useful.

Tyrone Daulton


Bob Harris wrote:

}
}
} Hello All:
}
} ... We are working primarily with iron compounds and would like to be
} able to recognize
} different valency states formed during incubation with microbes.
} What I need is a course and/or fundamental reference material to
} ease me into this. Is anyone aware of of something dealing with
} EELS spectra that I could take advantage of somewhere in North
} America?
}
} Bob Harris
} NSERC Regional STEM Facility
} Dep't of Microbiology
} University of Guelph
} Guelph Ontario
} Canada N1G 2W1
} Phone: 519-824-4120 ext 6409
} Fax: 519-837-1802

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Facility
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Thu Apr 11 08:29:24 2002



From: J-H Lignot :      J-H.Lignot-at-c-strasbourg.fr (by way of
Date: Thu, 11 Apr 2002 08:11:31 -0500
Subject: adapter for a Bausch & Lomb "Balplan"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear members,

I am looking for a C-mount camera adapter that could be fitted on a
Bausch & Lomb Balplan micrsocope. Any help would be very welcome....

Cheers, JH.
Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938


From daemon Thu Apr 11 10:09:02 2002



From: Siegfried Jaecques :      Siegfried.Jaecques-at-mech.kuleuven.ac.be
Date: Thu, 11 Apr 2002 16:59:44 +0200
Subject: Re-embedding bone in methylmethacrylate blocks (?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers

We have a set of guinea pig tibia fragments (about 15 mm long, 4-5 mm
diameter) embedded in poly-methylmethacrylate (PMMA) resin, catalyst cured
at moderate temperature (about 50 degrees C). Unfortunately, many air
bubbles are now trapped in the cured blocks (which does not usually happen
with the protocol we use).
Is there a way to remove the specimens from the PMMA blocks and re-embed
them properly in PMMA? I searched the archives and some other sources, but I
only found a question from 1999 (quoted below) that was apparently not
answered with a copy to the list.

} 3 Dec 1999 19:26:38 GMT -0400
..
} Does anyone have a protocol to remove methacrylate
} plastic from tissue sections using 1-acetoxy-2-methoxyethane?

Any help (e.g. pointers to literature, or maybe a protocol for MMA removal?)
would be greatly appreciated.
Thank you

dr. ir. Siegfried V.N. Jaecques
K.U. Leuven
Division of Biomechanics and Engineering Design (BMGO)
Celestijnenlaan 200 A
B-3001 Leuven (BELGIUM)




From daemon Thu Apr 11 10:39:49 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Thu, 11 Apr 2002 11:33:03 -0400 (EDT)
Subject: Large format scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all...

Sorry if this topic is off the beaten path. Like many of you, our EM
facility is also becoming a repository for other types of digital imaging
and printing technologies (i.e large format printers and scanners).

We are looking into acquiring a large format scanner. It seems that the
only models that are in existence at the moment are sheetfed style
scanners (up to about 54 inches maximum scan area). I've tracked down a
larger flatbed style by Microtek, but it appears to have been
discontinued. The largest true flatbed scanner I have been able to find
is around 12x18 inches. Is anyone aware of any flatbed scanner that is
larger than that?

Very best regards,

Angela
-----------------------------------------
**Please note new department name**

Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------



From daemon Thu Apr 11 12:19:03 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Thu, 11 Apr 2002 13:10:47 -0400
Subject: RNAse Free Section Adhesives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


try glow discharge treating the films this will make them very hyrophilic.


From daemon Thu Apr 11 13:35:00 2002



From: William Perreault :      william.j.perreault-at-lawrence.edu
Date: Thu, 11 Apr 2002 12:26:47 +0000
Subject: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is almost embarrassing to ask, but I run a teaching lab for
undergraduate students and we have accumulated literally hundreds of
stubs with double sticky carbon mounts. I would love to clean them up
and start all over, but the prospect of scraping the tapes off with a
razor blade is daunting. Can anyone suggest a solvent or alternate
procedure? Thanks in advance.


From daemon Thu Apr 11 15:46:00 2002



From: LIU, JINGYUE [AG/1000] :      jingyue.liu-at-Monsanto.com
Date: Thu, 11 Apr 2002 15:36:13 -0500
Subject: Immediate Open for a Postdoctoral Research Fellow

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

The following position is immediately available at Monsanto Company in St.
Louis, Missouri.
----------------------------------------------------------------------------
--------------------------------------------------------------

Department: Research & Development
Title: Postdoctoral Research Associate
Req Number: mons-00000241
Location(s): St. Louis MO

Responsibilities:
A two-year postdoctoral fellow position is available immediately for
developing, implementing, and applying advanced microscopy techniques to
elucidating the ultrastructure of plants, seeds, weeds and other biological
systems. Additionally, the selected individual is responsible for developing
new methods to improve the sample preparation protocols of biological
systems. The selected candidate will interact with multifunctional groups of
scientists working on biotechnology projects.

Required Skills:
The position requires a Ph.D. in plant biology or a related field with
experience in advanced electron and light microscopy techniques. A strong
background and extensive experience in TEM, high-resolution cryo-SEM, and
confocal laser scanning microscopy techniques are essential. Experience with
gene transformation in plants is a strong plus. The following key
competencies are desired: highly motivated and interested in developing new
imaging technologies; good interpersonal, verbal and written communication
skills; innovative and seeking opportunity to improve existing techniques
and processes.

To respond to this job, access our website -at-: www.monsanto.com

Please be sure to select the proper source!

Monsanto values diversity and is an equal opportunity affirmative action
employer.






Jingyue Liu
Science Fellow
Monsanto Company
800 N. Lindbergh Blvd., U1E
St. Louis, Missouri 63167
(314) 694-2408
jingyue.liu-at-monsanto.com


From daemon Thu Apr 11 16:02:20 2002



From: Visitec-at-t-online.de (Martin Klein)
Date: Thu, 11 Apr 2002 22:52:52 +0200
Subject: AW: Large format scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hallo Angela,

I like these topics which are asking for superlative machines. I found the
largest flatbed scanner in the market with an active working area of 36" x
48". Try the url http://www.purup-eskofot.com/eskoscan/

There is no commercial interest from me in this link. I was just curious
about the maximum.

Greetings from Germany

Martin Klein


VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen/Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: mklein-at-visitec-em.de
WWW: http://www.visitec-em.de
+ + + Home of the world's largest SEM + + +

-----Ursprüngliche Nachricht-----
Von: Angela Klaus [mailto:avklaus-at-amnh.org]
Gesendet: Donnerstag, 11. April 2002 17:33
An: microscopy-at-sparc5.microscopy.com
Betreff: Large format scanners


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi all...

Sorry if this topic is off the beaten path. Like many of you, our EM
facility is also becoming a repository for other types of digital imaging
and printing technologies (i.e large format printers and scanners).

We are looking into acquiring a large format scanner. It seems that the
only models that are in existence at the moment are sheetfed style
scanners (up to about 54 inches maximum scan area). I've tracked down a
larger flatbed style by Microtek, but it appears to have been
discontinued. The largest true flatbed scanner I have been able to find
is around 12x18 inches. Is anyone aware of any flatbed scanner that is
larger than that?

Very best regards,

Angela
-----------------------------------------
**Please note new department name**

Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------




From daemon Thu Apr 11 16:59:41 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 11 Apr 2002 16:51:33 -0500
Subject: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


William,

I used to soak them en masse in acetone, but it's still a messy process. It makes the adhesive mounts come off easier, but there's still a lot of scraping, etc.

My suggestion: you have all these undergrads at your disposal......hmmmmm. :-)

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: William Perreault [mailto:william.j.perreault-at-lawrence.edu]
Sent: Thursday, April 11, 2002 7:27 AM
To: microscopy-at-sparc5.microscopy.com


This is almost embarrassing to ask, but I run a teaching lab for
undergraduate students and we have accumulated literally hundreds of
stubs with double sticky carbon mounts. I would love to clean them up
and start all over, but the prospect of scraping the tapes off with a
razor blade is daunting. Can anyone suggest a solvent or alternate
procedure? Thanks in advance.



From daemon Thu Apr 11 18:11:44 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 11 Apr 2002 16:03:07 -0700 (PDT)
Subject: Environmental SEM with 0.4 M Na2CO3

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I have someone that is interested in using either Na2CO3 or Na2PO4 (0.4 M
in water) as a gas for our Environmental SEM. I was wondering if anyone
had any experience in working with this in an environmental SEM, or other
gases.
Thanks.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Thu Apr 11 19:17:20 2002



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 11 Apr 2002 19:08:27 -0700
Subject: RE: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Naptha is the best solvent I've found for all non-hardening adhesives.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Thursday, April 11, 2002 5:27 AM, William Perreault [SMTP:william.j.perreault-at-lawrence.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This is almost embarrassing to ask, but I run a teaching lab for
} undergraduate students and we have accumulated literally hundreds of
} stubs with double sticky carbon mounts. I would love to clean them up
} and start all over, but the prospect of scraping the tapes off with a
} razor blade is daunting. Can anyone suggest a solvent or alternate
} procedure? Thanks in advance.
}
}
}



From daemon Thu Apr 11 19:49:27 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 11 Apr 2002 20:43:02 -0400
Subject: Looking for a Locator Slide

Contents Retrieved from Microscopy Listserver Archives
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Can't find a replacement anywhere, so I'm asking.

Does anyone know where one might acquire what was one called a
"Micro-Locator" slide. The one that was just broken was obtained from
Scientific Products way back when.........I was the only one who would use
it. Add one more person and see what happens. "Oops!" On the floor.
Fractured beyond any repair.

Sure would appreciate some help. You know the slide you place on the stage
of the microscope on which you see that cell-of-cells to get a coordinate.
Then you place the locator slide on top of the specimen slide and locate the
coordinates on the confocal LM. Remove the locator and you have your cell
back. Well, some of the time anyway.

Thanks again all and have a nice weekend,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin/wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Thu Apr 11 20:01:21 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Thu, 11 Apr 2002 18:16:45 -0700
Subject: Large format scanners

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Jim (or anyone from MVIA)

I'm trying to contact you about these adaptors, my emails keep
bouncing back, both to haley-at-mvia.com and to info-at-mvia.com.
I can get to your website OK, though.

Can you please contact me by email from an address that I can reply
to?

My apologies to the 3,000 or so other people who will receive this.

thanks

rtch






Angela;
I just saw a HUGE scanner today made by Fuji, but I didn't get the
model #. It did reflection and transparencies at 2800 DPI. It was around
18"x24" and was for commercial labs doing things like scanning up to 50 35mm
slide transparencies simultaneously.

John Mardinly
Desk: 765-2346
Pager: 322-6490
Field Emission TEM:765-8101
LaB6 TEM: 765-8253
FIB: 765-8247
TEM Prep: 765-2467



-----Original Message-----
} From: Angela Klaus [mailto:avklaus-at-amnh.org]
Sent: Thursday, April 11, 2002 8:33 AM
To: microscopy-at-sparc5.microscopy.com


Hi all...

Sorry if this topic is off the beaten path. Like many of you, our EM
facility is also becoming a repository for other types of digital imaging
and printing technologies (i.e large format printers and scanners).

We are looking into acquiring a large format scanner. It seems that the
only models that are in existence at the moment are sheetfed style
scanners (up to about 54 inches maximum scan area). I've tracked down a
larger flatbed style by Microtek, but it appears to have been
discontinued. The largest true flatbed scanner I have been able to find
is around 12x18 inches. Is anyone aware of any flatbed scanner that is
larger than that?

Very best regards,

Angela
-----------------------------------------
**Please note new department name**

Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------



From daemon Thu Apr 11 20:56:40 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Thu, 11 Apr 2002 21:49:56 -0400 (EDT)
Subject: RE: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
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As Randy suggested, soaking (with maybe some sonication) in acetone gets
most of the gunk off. After that, rather than scraping, you might try
cleaning off the rest of the residue using a large grain polishing paper.
Just whiz the stubs around on the paper (or maybe use a wheel). I used to
do this (Eons ago. And wear gloves, as Randy says, it's still messy) and
it worked well.

Best,

Angela
-----------------------------------------
**Please note new department name**

Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------

On Thu, 11 Apr 2002, Tindall, Randy D. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} William,
}
} I used to soak them en masse in acetone, but it's still a messy process. It makes the adhesive mounts come off easier, but there's still a lot of scraping, etc.
}
} My suggestion: you have all these undergrads at your disposal......hmmmmm. :-)
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}
} -----Original Message-----
} } From: William Perreault [mailto:william.j.perreault-at-lawrence.edu]
} Sent: Thursday, April 11, 2002 7:27 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: too many aluminum stubs
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This is almost embarrassing to ask, but I run a teaching lab for
} undergraduate students and we have accumulated literally hundreds of
} stubs with double sticky carbon mounts. I would love to clean them up
} and start all over, but the prospect of scraping the tapes off with a
} razor blade is daunting. Can anyone suggest a solvent or alternate
} procedure? Thanks in advance.
}
}
}



From daemon Thu Apr 11 21:05:41 2002



From: Berg, R. Howard :      RHBerg-at-danforthcenter.org
Date: Thu, 11 Apr 2002 16:54:39 -0500
Subject: wax microtome

Contents Retrieved from Microscopy Listserver Archives
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List,

We are planning to set up a lab to paraffin-embed and section plant
tissues. This is to solicit recommendations for wax microtomes and
ancillary equipment that might be appropriate for such lab. Thanks in
advance for your advice.


R. Howard Berg, Ph.D.
Director, Integrated MIcroscopy Facility
Danforth Plant Science Center
975 N. Warson Rd.
St. Louis, MO 63132

ph 314-587-1261 fx 314-587-1361 cell 314-378-2409
rhberg-at-danforthcenter.org www.danforthcenter.org


From daemon Thu Apr 11 23:09:20 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 11 Apr 2002 23:57:22 -0500
Subject: Cleaning of SEM mounts

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Angela Klaus wrote:
================================================================
As Randy suggested, soaking (with maybe some sonication) in acetone gets
most of the gunk off. After that, rather than scraping, you might try
cleaning off the rest of the residue using a large grain polishing paper.
Just whiz the stubs around on the paper (or maybe use a wheel). I used to do
this (Eons ago. And wear gloves, as Randy says, it's still messy) and it
worked well.
================================================================
We here at SPI Supplies and Structure Probe, Inc. have thought about this
problem for more than thirty years. You really have to be careful when you
are doing this cleaning, scraping, and grinding and sandpapering. Even
within our small company, early on we learned that it just seemed to be
impossible to keep track of what kinds of samples go into that waste mount
box. Some samples could contain toxic materials if airborne as particles.
My big fear in our own laboratory after a near miss in the mid-1970's was
that beryllium mounts (planchetts) could get mixed in with the "waste" mount
box and end up getting sanded down along with the aluminum mounts.

So if you do plan to recycle the mounts, a practice we would like to
encourage in fact, we suggest being careful to segregate the mounts into
several different categories, for example, biohazards, materials science
hazards, inerts, but under no circumstances, let any beryllium planchetts
get mixed in with the mounts for recycling. That way, you could recycle
those with inert samples, but for those that would still pose a risk, you
could dispose of them through your waste disposal program.

Disclaimer: SPI Supplies is one of the main manufactures of SEM mounts for
all SEMs. Although we would always like to sell more mounts, we are a firm
believer in the concept of recycling, but want to make sure that these
dangers are not forgotten. These are the same dangers that have kept us
from introducing a mount recycling program of our own, as a commercial
service: We could not assure the safety of our own employees with regard to
what they might be exposed when cleaning mounts from outside our own
organization.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Fri Apr 12 00:25:14 2002



From: Skip :      allcs-at-earthlink.net
Date: Fri, 12 Apr 2002 01:16:44 -0400
Subject: Q from http://www.msa.microscopy.com/Ask-A-Microscopist.html

Contents Retrieved from Microscopy Listserver Archives
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} } }
} } } }
} } } } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } } } submitted by (allcs-at-earthlink.net) from
} } } } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
} } } } April 11, 2002 at 10:35:35
} } }
}
} -------------------------------------------------------------------------
-
} } -
} } } }
} } } } Email: allcs-at-earthlink.net
} } } } Name: Dr. Skip Coppola
} } } }
} } } } Organization: allcs
} } } }
} } } } Education: Graduate College
} } } }
} } } } Location: atlanta, GA USA
} } } }
} } } } Question: I have inherited a small microscope recently. The 50x
} } } } objective is flat, with the lense slightly recessed, and it will not
} } } } focus without touching or pressing the subject slide. I believe it
} } } } might be an oil immersion lense. How can I verify this? Will testing
} } } } with immersion oil damage a non-oil lense?
} } } }
} } } } Thanks.
} } } }
} } }
}
} -------------------------------------------------------------------------
-
} } -
} } }
}



From daemon Fri Apr 12 01:34:39 2002



From: DETLEF.WARMBOLD-at-LHT.DLH.DE
Date: Fri, 12 Apr 2002 08:26:31 +0200
Subject: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
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The best way is to grind them on a grinding wheel, that's what we do.
Acetone wont relay help it only makes a big mess.

Best regards


Lufthansa Technik AG

Detlef Warmbold
Materials and Process Technology
HAM TQ 23


-----Original Message-----
} From: William Perreault [mailto:william.j.perreault-at-lawrence.edu]
Sent: Thursday, April 11, 2002 2:27 PM
To: microscopy-at-sparc5.microscopy.com


This is almost embarrassing to ask, but I run a teaching lab for
undergraduate students and we have accumulated literally hundreds of
stubs with double sticky carbon mounts. I would love to clean them up
and start all over, but the prospect of scraping the tapes off with a
razor blade is daunting. Can anyone suggest a solvent or alternate
procedure? Thanks in advance.


From daemon Fri Apr 12 01:43:22 2002



From: jesper.v.carstensen-at-risoe.dk
Date: Fri, 12 Apr 2002 08:37:14 +0200
Subject: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
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Dear William,

When we have numerous stubs with carbon mounts, which need cleaning, we
usually put them in an oven for 1/2-1 hour at 100-150°C. This usually makes
the carbon mounts come off much easier.

Kind regards,
Jesper
____________________________________
Jesper Vejloe Carstensen
Risoe National Laboratory
Materials Research Department
Frederiksborgvej 399, P.O. Box 49
DK-4000 Roskilde, Denmark
Phone: +45 4677 5776 Fax: +45 4677 5758
E-mail: jesper.v.carstensen-at-risoe.dk
Web: www.risoe.dk/afm


-----Original Message-----
} From: William Perreault [mailto:william.j.perreault-at-lawrence.edu]
Sent: 11. april 2002 14:27
To: microscopy-at-sparc5.microscopy.com


This is almost embarrassing to ask, but I run a teaching lab for
undergraduate students and we have accumulated literally hundreds of
stubs with double sticky carbon mounts. I would love to clean them up
and start all over, but the prospect of scraping the tapes off with a
razor blade is daunting. Can anyone suggest a solvent or alternate
procedure? Thanks in advance.


From daemon Fri Apr 12 02:49:33 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 12 Apr 2002 08:41:58 +0100 (GMT Daylight Time)
Subject: Re: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
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I give them to new staff and work experience students to
scrape. Who says power doesn't corrupt? :)

Dave

PS It is less daunting if you just scrape the number you
need daily.


On Thu, 11 Apr 2002 12:26:47 +0000 William Perreault
{william.j.perreault-at-lawrence.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This is almost embarrassing to ask, but I run a teaching lab for
} undergraduate students and we have accumulated literally hundreds of
} stubs with double sticky carbon mounts. I would love to clean them up
} and start all over, but the prospect of scraping the tapes off with a
} razor blade is daunting. Can anyone suggest a solvent or alternate
} procedure? Thanks in advance.
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Apr 12 07:59:24 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Fri, 12 Apr 2002 08:50:23 -0400
Subject: RE: too many aluminum stubs

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have you considered just throwing them out?


From daemon Fri Apr 12 08:02:49 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 12 Apr 2002 09:01:32 -0400
Subject: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
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William;

If you have a small ultrasonic lab. degreaser put them in there with
acetone. You'll need several cycles of this to get them clean but it's
better than spending time with the razor blade. 2-propanol works well too
followed by acetone and be certain they are very dry by putting them in an
oven for 24 hrs. or your vacuum will be affected.

Peter Tomic

-----Original Message-----
} From: William Perreault [mailto:william.j.perreault-at-lawrence.edu]
Sent: Thursday, April 11, 2002 8:27 AM
To: microscopy-at-sparc5.microscopy.com


This is almost embarrassing to ask, but I run a teaching lab for
undergraduate students and we have accumulated literally hundreds of
stubs with double sticky carbon mounts. I would love to clean them up
and start all over, but the prospect of scraping the tapes off with a
razor blade is daunting. Can anyone suggest a solvent or alternate
procedure? Thanks in advance.


From daemon Fri Apr 12 08:25:38 2002



From: Heeschen, Bill (WA) :      WAHEESCHEN-at-dow.com
Date: Fri, 12 Apr 2002 09:15:07 -0400
Subject: Environmental SEM with 0.4 M Na2CO3

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I'm a bit confused here about the purpose of the salt solutions as the
source of the "gas". Is the salt solution intended to regulate the vapor
pressure of the water? A scrubber for incoming gas? or is it simply present
in the sample (like a buffer solution)? It seems like the salt solution
would only provide water as the gas. I could see where you might get a
minor little bit of CO2 from the Na2CO3 if you were trying to modify the gas
in the chamber, but I don't think there would be any substantial gas-phase
component (other than H2O) from the phosphate solution. Also, I am guessing
that the phosphate solution is Na2HPO4 (or Na3PO4). I'm a bit rusty on my
inorganic chemistry, but I think these salts are based on the hydration of
P2O5 which has a fairly low vapor pressure.

I will assume that these solutions are simply present in the sample. It
seems to me that there should not be any interference until you start to
evaporate quite a bit of the water, then you will start to see the effects
of the concentrated salts - either crystals/residue forming on the specimen
or the solutions will get oily as the salt holds onto the water with
increasing tenacity!

Hope this helps.

Bill
William A. Heeschen
The Dow Chemical Company
Microscopy, Digital Imaging
1897 Bldg, E-84 / 2040 Bldg, 1330
waheeschen-at-dow.com


-----Original Message-----
} From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU]
Sent: Thursday, April 11, 2002 7:03 PM
To: microscopy-at-sparc5.microscopy.com


Hello,
I have someone that is interested in using either Na2CO3 or Na2PO4 (0.4 M
in water) as a gas for our Environmental SEM. I was wondering if anyone
had any experience in working with this in an environmental SEM, or other
gases.
Thanks.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
\
Gordon Ante Vrdoljak Electron Microscope
Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Apr 12 08:25:38 2002



From: Ping Li :      pli-at-is.dal.ca
Date: Fri, 12 Apr 2002 10:18:02 -0300
Subject: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
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I am able to clean large amount of stubs easily. The stubs look almost like
new after cleaning. The method I used was just as many of you suggested.
First, soak the stubs in acetone and sonicate them for about three hours (I
do about 150 stubs a time). During the soaking and sonicating period, stir
the stubs several times and change acetone a couple of times. When all the
carbon mounts are off from the stubs and most of the gunk are off, I wash
the stubs with sparkleen (from FISHER). During the wash, I used a large
brush to stir/brush all the stubs together for about five minutes. This will
get all remaining gunk off the stubs. Use running water to wash off
sparkleen. Finally, I soak the stubs in acetone and sonicate them for
another 5-10 minutes. The stubs are now clean and ready to be used again. I
am able to reuse the stubs several times with this clean method.

Ping

--
Ping Li, Ph.D.
Director, Scientific Imaging Suite
Department of Biology
Dalhousie University
Halifax, NS B3H 4J1
Canada
Tel: 902-494-3309
Fax: 902-494-3736
E-mail: Ping.Li-at-Dal.Ca



-----Original Message-----
} From: William Perreault [mailto:william.j.perreault-at-lawrence.edu]
Sent: Thursday, April 11, 2002 9:27 AM
To: microscopy-at-sparc5.microscopy.com


This is almost embarrassing to ask, but I run a teaching lab for
undergraduate students and we have accumulated literally hundreds of
stubs with double sticky carbon mounts. I would love to clean them up
and start all over, but the prospect of scraping the tapes off with a
razor blade is daunting. Can anyone suggest a solvent or alternate
procedure? Thanks in advance.




From daemon Fri Apr 12 09:32:28 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 12 Apr 2002 09:23:56 -0500
Subject: RE: Environmental SEM with 0.4 M Na2CO3

Contents Retrieved from Microscopy Listserver Archives
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You will not have salts in chamber atmosphere in significant amounts. Just water will evaporate.

Vladimir



} -----Original Message-----
} From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU]
} Sent: Thursday, April 11, 2002 6:03 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Environmental SEM with 0.4 M Na2CO3
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hello,
} I have someone that is interested in using either Na2CO3 or
} Na2PO4 (0.4 M
} in water) as a gas for our Environmental SEM. I was
} wondering if anyone
} had any experience in working with this in an environmental
} SEM, or other
} gases.
} Thanks.
} Gordon
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
} \/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak
} Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085
} Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
}
}


From daemon Fri Apr 12 09:44:52 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 12 Apr 2002 09:38:52 -0500
Subject: Re: Environmental SEM with 0.4 M Na2CO3

Contents Retrieved from Microscopy Listserver Archives
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Unless you can vaporize the Na2CO3 or Na2PO4, I would not try to introduce
it as a gas. Whatever residue would remain from evaporation in a dish on
your bench top would end up somewhere inside your microscope.

If they wanted to set the sample in a pool of the salt, that would be
another question. It might be okay unless you get salt crystals forming on
the sample surface.

I suggest your someone rethink their situation. I would guess they have a
sample saturated with such a solution. It is not the salt that would
evaporate in the vacuum of the E-SEM; it is the water, which by evaporating
would leave a more concentrated solution. Therefore, maintaining water
vapor should accomplish what they really want to do.

Now to extend that question to where it might matter - how would you handle
it if you have a mixture of two volatile species, say propanol and water?
Would you not want to feed a gas that would have both species present in
proportion to their vapor pressure under the scope conditions? That would
not necessarily be the same as their proportion in the liquid.

Warren

At 04:03 PM 4/11/02 -0700, you wrote:

} Hello,
} I have someone that is interested in using either Na2CO3 or Na2PO4 (0.4 M
} in water) as a gas for our Environmental SEM. I was wondering if anyone
} had any experience in working with this in an environmental SEM, or other
} gases.
} Thanks.
} Gordon
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Fri Apr 12 10:15:20 2002



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 12 Apr 2002 10:04:56 -0500
Subject: Re: wax microtome

Contents Retrieved from Microscopy Listserver Archives
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Berg, R. HowardRHBerg-at-danforthcenter.org4/11/02 4:54 PM

Dr. Berg,

On your request for ancillary equipment, consider getting a laboratory
microwave oven for processing. We are just beginning to attempt to master
and use the relatively new microwave oven assisted techniques for paraffin
embedding, mounting of sections onto slides, and staining sections of plant
tissues. In addition to the claimed as good or better preservation of
morphology, the total time involved in preparation is reduced from 7-9 days
to something like 5 hours or so. We are just gearing up for our first
attempts, so no results yet.

Two references for this are:

1. Microwave Techniques and Protocols, edited by Richard T. Giberson and
Richard Demaree, Humana Press (2001), Chapter 15 "Microwave Paraffin
Techniques for Botanical Tissues, by Denise Schichnes, Jeffrey A. Nemson,
and Steven E. Ruzin.

2. Schichnes D, Nemson J, Ruzin, SE (1998) Microwave protocols for paraffin
microtechnique and in situ localization in plants. Microscopy &
Microanalysis, 4:491-496. [has great color photos]

You might want to consider looking into this approach as you begin to set up
your new lab. Good luck!

Have any other Listers out there usede MW assisted paraffin embedding, and
what kinds of results have you had?

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/

} List,
}
} We are planning to set up a lab to paraffin-embed and section plant
} tissues. This is to solicit recommendations for wax microtomes and
} ancillary equipment that might be appropriate for such lab. Thanks in
} advance for your advice.
}
}
} R. Howard Berg, Ph.D.
} Director, Integrated MIcroscopy Facility
} Danforth Plant Science Center
} 975 N. Warson Rd.
} St. Louis, MO 63132
}
} ph 314-587-1261 fx 314-587-1361 cell 314-378-2409
} rhberg-at-danforthcenter.org www.danforthcenter.org



From daemon Fri Apr 12 10:37:05 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 12 Apr 2002 10:30:44 -0500
Subject: Expiration dates for reagents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

Is anyone aware of a source listing generally accepted expiration periods for laboratory reagents? I'm mainly concerned, of course, with those used in electron microscopy. We are looking into Good Laboratory Practice (GLP) standards, and while we monitor dates on our chemicals, fixatives, stains, etc., I'm wondering if there are "official" figures out there somewhere.

Some chemicals have expiration dates on the original packaging, but not all. And how about mixed solutions?

Any help would be greatly appreciated.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Fri Apr 12 10:37:05 2002



From: Manoj Misra :      Manoj.Misra-at-unilever.com
Date: April 16, 2002
Subject: APRIL 16th: Metro. Micros. Soc. Meeting 2nd Notice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

The next meeting of the Metropolitan Microscopy Society will be held on April
16th, 2002 at the LEO US headquarters facility in Thornwood, NY. LEO has
graciously agreed to host our meeting and to provide complimentary coffee and
lunch to all attendees.

The meeting itself will comprise a technical symposium of six presentations
of interest to both photon and electron microscopists. The talks will cover a
broad range of topics including molecular resolution via cryo -FESEM,
cell imaging at the bottom ( {100nm) of cells, quantitative X-ray analysis
using ESEM, as well SEM based metrology methods / techniques and discussions.

We invite all members to attend and to inform their colleagues and fellow
workers who may also wish to attend.

Due to the nature of the corporate facility the meeting will be held at, it is
essential that members pre-register so that an attendee list can be delivered
to the security people at LEO for generation of guest badges. It will also
help us plan for lunches.

The pre-registration deadline is April 12 and can be accomplished
electronically. Please respond via email or fax to EVAN SLOW ( ESS-at-FEICO.com)
directly. A simple email note is all that’s required to pre-register. You can
then bring the required fee with
you to the meeting. For all attendees, the meeting fee, which includes lunch,
will be $20.00.

PROGRAM

Metropolitan Microscopy Society
Spring Meeting 2002


Time: 9:00 am (registration begins)

Place: LEO, One Zeiss Drive, Thornwood, NY (914) 747- 7700

Directions: {http://www.leo-usa.com/} (click visiting LEO, Thornwood, US)

9:00 - 9:45 Registration

9:45 - 10:00 Introductory Remarks (Al Sicignano).

10:00 - 10:45 "Molecular Resolution with Cryo-Field Emission SEM:
Visualization of Individual CAMs (P-selectin, GpIX/GpIB, and GpIIb/IIIa) in the
Glycocalyx of Human Platelets, Dr. Stanley L. Erlandsen, Univ. of Minnesota
Medical School, Minneapolis, MN 55455

10:45 - 11:30 "Overview of NIST Programs Related to SEM Metrology” Dr. Andras
Vladar, Nanoscale Metrology Group, NIST, Gaithersburg, MD

11:30 - 12:15 “Flashy Fireworks: Imaging at the Bottom {100 Nanometers of the
Cell, Dr. Derek Toomre, Yale University School of Medicine, New Haven, CT

12:15 - 1:00 Lunch and facility tour (included with registration - please
pre-register!)

1:00 - 1:45 “ESEM Can Produce Quantitative X-ray Results”, Dr. Charles Lyman,
Lehigh University, Bethlehem, PA

1:45 - 2:30 " Issues Affecting SEM Measurement Precision”, Al Sicignano,
Nanometrology LLC, Ardsley, NY

2:30 - 3:15 " Application Techniques Results”, David Frey, LEO, Thornwood,
NY.


M. Misra

Manoj Misra, Ph.D.
Group Leader
Advanced Imaging & Measurement
Unilever Research
Edgewater, NJ 07020
Phone: (201) 840-2702
Fax: (201) 840-8269







From daemon Fri Apr 12 11:46:11 2002



From: Brian Wajdyk :      electronmicroscopist-at-hotmail.com
Date: Fri, 12 Apr 2002 09:37:55 -0700
Subject: Backscattered Electron Detector - recommendation / explanation

Contents Retrieved from Microscopy Listserver Archives
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Fri, 12 Apr 2002 16:37:55 GMT
X-Originating-IP: [65.197.242.21]


Fellow Microscopists,

I currently have a Backscattered Electron Detector from a major
manufacturer, who I will not name, which I am not happy with. Let's suffice
it to say that it is a scintillator type detector that Hitachi likes to
recommend. I have a Hitachi 4100 (which is similar to a 4500 or 4700
without the in-lens detector) that I use for failure analysis of
semiconductors. I would like to mention that I used to have the same
detector on two other Hitachi 4500 FESEM's that were also ineffective. I
have two problems with the detector. First, it has a poor signal to noise
ratio. I find that it is not as good as the BSE detector I used to have on
my old Cambridge 250 and 360 series SEM's. Second, its shape is not
conducive to simultaneous EDS and BSE analysis. I can only do both if I do
not insert the BSE detector all they way. This isn't acceptable because I'm
looking for atomic element contrast not topographical.

I have looked around a bit and found ETP, GW electronics, and Autrata as the
main manufactures. Can anyone tell me about their experiences and
recommendations with their BSE detectors? I'm a bit fuzzy on the benefits
and limitations of the scintillator, solid state, and YAG detectors. I
would appreciate it if someone could straighten me out on this.

Thank you in advance,

Brian Wajdyk

*************************************************
Brian Wajdyk
Sr. Microscopist, Electron Optics Lab Supervisor
On Semiconductor
5005 E. McDowell Rd.
Phoenix, AZ 85008
Mail Drop B132
Ph: 602-244-4883 Pgr: 877-585-9946
*************************************************




_________________________________________________________________
Chat with friends online, try MSN Messenger: http://messenger.msn.com



From daemon Fri Apr 12 11:46:12 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 12 Apr 2002 09:40:30 -0700
Subject: Re: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear William,
I have the same problem sometimes,when the undergraduates have been
especialy busy. My routine is: soak in acetone for 1 to 2 hours in a beaker.
Sonicate for two minutes, lift each one out with forceps and wipe the sticky
tab, now quite slimy, off on a paper towel. If they have been sufficiently
soaked, they wipe right off.
At 12:26 PM 4/11/2002 +0000, you wrote:
}
} This is almost embarrassing to ask, but I run a teaching lab for
} undergraduate students and we have accumulated literally hundreds of
} stubs with double sticky carbon mounts. I would love to clean them up
} and start all over, but the prospect of scraping the tapes off with a
} razor blade is daunting. Can anyone suggest a solvent or alternate
} procedure? Thanks in advance.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Apr 12 12:04:21 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 12 Apr 2002 12:58:31 -0400
Subject: URL's for Micro-Slide Locator Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


First, thanks to those who answered.

For the information of all, these are the results of the Locator Slide
search.

Gurley Precision Optics, http://www.gurley.com [$80][0.1mm squares]
Lovins Micro-Slide Field finder:
http://www.gpi-optics.com/Field_Finder.pdf

McCrone, http://www.mccrone.com/mac/home2.html [$150][1mm squares]*,**
England Field Finder:
http://www.mccrone.com/cgi-bin/SoftCart.exe/mac/supplies/fieldfind.html?L+mc
crone+jven1292+1018639553

*McCrone promises that their finders are precisely interchangeable.
**McCrone also has Reflective Stage Micrometers

Regards to all,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin/wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Fri Apr 12 13:11:36 2002



From: Steve Beck :      becks-at-sunynassau.edu
Date: Fri, 12 Apr 2002 14:04:10 -0400
Subject: Kodak 4127 Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I have been using Kodak Professional film (4127) for many years in my SEM
course. I have recently been informed that this film has been discontinued
by Kodak. Does anyone know of a good replacement for this film?

Thanks for your advice!

Steve

Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Fri Apr 12 13:11:37 2002



From: Steve Beck :      becks-at-sunynassau.edu
Date: Fri, 12 Apr 2002 13:58:55 -0400
Subject: Summer 2002 - SEM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SUMMER I 2002 COURSE ANNOUNCEMENT - Scanning Electron Microscopy
(BIO. 222-Section BA)

NASSAU COMMUNITY COLLEGE, Garden City, Long Island, New York

A five week, Summer Session I, 2002 semester, course in Biological Scanning
Electron Microscopy is being offered by the Biology Department of Nassau
Community College. This is a 4 credit course offered four days per week
(Monday through Thursday) between the hours of 8:00 am and NOON. Classes
will begin on May 28 and end on June 27, 2002.

This is a "hands-on" course emphasizing biological specimen preparation,
student operation of the SEM (Hitachi S-2400) and the production of
electron micrographs through the process of black & white photography and
digital image capture, along with electron micrograph analysis. Students
will work on a number of biological samples with the goal of producing a
portfolio of high quality SEM photomicrographs.

The course is widely transferrable and the cost per credit is reasonable at
$100 per credit (for Nassau County residents or New York State residents
with a certificate of residency).

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and student gallery of EM photomicrographs is available at our
web site. The URL is
{http://www.sunynassau.edu/webpages/biology/becks.htm} .

Interested individuals should register as soon as possible since the course
is limited to a total enrollment of ten (10) students.

If you have further questions, you should e-mail me directly at the address
below.

For information about mail or telephone registration (Dial-a-Course) point
your browser to http://www.sunynassau.edu/courses/sum02/dialacourse.htm .
The phone registration option is available until 4/25/02 by calling
516-572-7131 or 7372 or 7425.

P.S. A Fall 2002 TEM course is also being offered (BIO 221 - Section E2) on
Thursday evenings beginning at 5:30 PM.


Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Fri Apr 12 13:18:16 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 12 Apr 2002 14:12:38 -0400
Subject: Re: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Acetone isn't the best solvent for tape adhesive. There is a commercial product called "Goop Remover" or something like that that works very well at softening adhesive. It is designed to remove the tags that stores and manufacturers put on nice shiny surfaces where you don't want the damn tags and they don't remove easily. I do not know what the main ingredient is, but I think that the product is available at K-mart, Wal-Mart and other hardware stores. If I can remember tonight, I will look at my can of the stuff and see if it says what's in it. That is what I would use first and then follow it with an acetone wash.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Friday, April 12, 2002 12:41 PM
To: William Perreault
Cc: Microscopy-at-sparc5.microscopy.com


Dear William,
I have the same problem sometimes,when the undergraduates have been
especialy busy. My routine is: soak in acetone for 1 to 2 hours in a beaker.
Sonicate for two minutes, lift each one out with forceps and wipe the sticky
tab, now quite slimy, off on a paper towel. If they have been sufficiently
soaked, they wipe right off.
At 12:26 PM 4/11/2002 +0000, you wrote:
}
} This is almost embarrassing to ask, but I run a teaching lab for
} undergraduate students and we have accumulated literally hundreds of
} stubs with double sticky carbon mounts. I would love to clean them up
} and start all over, but the prospect of scraping the tapes off with a
} razor blade is daunting. Can anyone suggest a solvent or alternate
} procedure? Thanks in advance.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Apr 12 13:59:24 2002



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Fri, 12 Apr 2002 14:52:59 -0400
Subject: Re: Interpreting EELS spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bob,
Two references particularly useful to your problem are:
Van Aken, P.A., Liebscher, B., and Styrsa, V.J. (1998a) Quantitative
determination of iron oxidation states in minerals using Fe L2,3-edge
electron energy-loss near-edge structure spectroscopy. Physics and
Chemistry of Minerals, 25, 323-327.
Van Aken, P.A., Styrsa, V.J, Liebscher, B. Woodland, A.B., and
Redhammer, G.J. (1999a) Microanalysis of Fe3+/SFe in oxide and
silicate minerals by investigation of electron energy-loss near-edge
structures (ELNES) at the Fe M2,3 edge. Physics and Chemistry of
Minerals, 26, 584-590.
They do not require superb resolution, although the Fe M2,3 edge can
have severe problems with overlap with other elements. Good Luck.
Ciao for now,
Ken


From daemon Fri Apr 12 14:22:24 2002



From: Clark Lantz :      lantz-at-email.arizona.edu
Date: Fri, 12 Apr 2002 12:17:21 -0700
Subject: Faculty Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a current opening for a faculty position in optical science at the
University of Arizona. The position is seeking an individual that can
develop a world class research effort in advanced optical microscopy. The
primary appointment will be in Optical Sciences with a joint appointment in
Physiology or Material Science. Interested applicants are referred to the
following URL for a complete description and application
instructions. Please respond to Dr. Dr. Jim Schwiegerling, Department of
Optical Sciences, Advanced Optical Microscopy Search Committee, The
University of Arizona, Tucson, Arizona 85721-0104.

Faculty Position Description:

http://www.hr.arizona.edu/22993xfacx.htm


Clark Lantz, Ph.D.
Professor and Associate Head
Cell Biology and Anatomy
University of Arizona
1501 N. Campbell Ave, Room LSN 447
PO Box 245044
Tucson, AZ 85724-5044

phone: 520-626-6716
FAX: 520-626-2097
email: lantz-at-email.arizona.edu


From daemon Fri Apr 12 16:17:14 2002



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Sat, 13 Apr 2002 07:09:21 +1000
Subject: digital cameras again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is there any difference between the same resolution cameras?

The most used looks to me is the Nikon Coolpix 950... isn't it? Anyone
experimented for example with Kodak 4900?

Keep care and be of good cheer

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
(title) 84 duke of Siebenlügner

websites:
http://www.coleoptera.org. and
http://www.egroups.com/group/coleoptera

University of Sydney
The Wentworth Bldg., B 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ICQ: 13610107

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).





From daemon Fri Apr 12 16:18:50 2002



From: joe.p.neilly-at-abbott.com
Date: Fri, 12 Apr 2002 16:12:10 -0500
Subject: Re: Expiration dates for reagents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Randy,

We have searched far and wide for this data and have NOT found any single
source. We have found the information in the literature, from manufacturers
and from other sources. If you find a reliable source for this information,
please let the list know. It would be very valuable for labs conforming to
GLP/GMP standards and those conforming to ISO standards.

Joe Neilly, Senior Microscopist
Abbott Laboratories
R45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027



"Tindall,
Randy D." To: {microscopy-at-sparc5.microscopy.com}
{TindallR-at-mis cc:
souri.edu} Subject: Expiration dates for reagents

04/12/02
10:30 AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear listers,

Is anyone aware of a source listing generally accepted expiration periods for
laboratory reagents? I'm mainly concerned, of course, with those used in
electron microscopy. We are looking into Good Laboratory Practice (GLP)
standards, and while we monitor dates on our chemicals, fixatives, stains,
etc., I'm wondering if there are "official" figures out there somewhere.

Some chemicals have expiration dates on the original packaging, but not all.
And how about mixed solutions?

Any help would be greatly appreciated.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/








From daemon Fri Apr 12 16:34:37 2002



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Fri, 12 Apr 2002 14:28:07 -0700
Subject: RE: Interpreting EELS spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



There is a recent paper on evaluation of Fe L23 EEL near-edge spectra in
Phys and Chem Minerals (2002), 29: 188-200 by P.A. van Aken.




From daemon Fri Apr 12 16:57:24 2002



From: sghoshro-at-NMSU.Edu
Date: Fri, 12 Apr 2002 15:55:48 -0600 (MDT)
Subject: RE: too many aluminum stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

"Throwing them out" sounds like the most unreasonable idea. Majority of
the university EM labs run on a very low budget and just like Randy and
probably many other people we regularly recycle all our aluminum stubs and
many other items in the lab. I think people should try their best to
recycle items that can be recycled and used again. Aluminum stubs
certainly fall in that category. If someone still prefers to throw them
out, then he/she should check with the organization safety office and they
may come up with a solution to have them recycled.

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml

On Fri, 12 Apr 2002, John Hoffpauir wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} have you considered just throwing them out?
}
}




From daemon Sat Apr 13 13:40:15 2002



From: William Perreault :      william.j.perreault-at-lawrence.edu
Date: Sat, 13 Apr 2002 13:24:39 +0000
Subject: many thanks for stub cleaning info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wow! Thank you all for the many and varied ideas on cleaning stubs. I
feel like the Sorcerers Apprentice--enough already! Seriously, thanks
and I shall try several of your suggestions. Bill P.


From daemon Sun Apr 14 08:26:41 2002



From: sstouden-at-thelinks.com
Date: Sun, 14 Apr 2002 08:15:43 -0500 (CDT)
Subject: Re: Imagining of Plakton

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



never thought about it until now ,but the new wide spectrum technology
which the government uses in its underground radar might be employed to
image these beasts of the sea? just a thought i think it is called GWS or
something likethat.
sterling

On Tue, 9 Apr 2002, Er Poh Nee (Yu Baoni) wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} We have lots of difficulties in trying to image unicellular plankton
} (flagellates)
}
} Our aim is to obtain as accurately as possible the surface area volume of
} such organism.
}
} We have tired to do optical sectioning using
} 1) autofluorescence
} 2) stain with acridine orange
} but the signal usually do not represent ( or stain) the entire volume.
}
} 3) transmitted mode
} but this is difficult to define the upper and lower limit of the organism.
}
} Do anyone have any good suggestions/ experiences? What are the suitable dyes
} to use?
}
} LiJia and other
}



From daemon Sun Apr 14 10:29:44 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Sun, 14 Apr 2002 11:21:00 -0400
Subject: Kodak 4127 Film

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try 4x5 inch tmax film from kodak, extreamly fine grained film.
john


From daemon Sun Apr 14 10:38:26 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Sun, 14 Apr 2002 11:32:39 -0400
Subject: RE: too many aluminum stubs

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Soumitra, one of the things I have learned over the years in that tech time is
far fmore expensive than supplies. If you have a tech making $15/hr and it
takes that long to recover the stubs then you are losing money. not to mention
the waste disopsal of the acetone or other reagents used to recycle them. I am
all for recycling, but only if it is trully cost effective. Since i don't know
what kind of stubs Mary is using i can't do a cost analysist. the prices range
from $1per to 0.20/ stub. In the end it depends on what works best for her
lab.
john


From daemon Sun Apr 14 10:41:05 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Sun, 14 Apr 2002 11:34:52 -0400
Subject: RE: Clean aluminum stubs

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is this method cost effective?


From daemon Sun Apr 14 10:53:48 2002



From: Beauregard :      beaurega-at-westol.com
Date: Sun, 14 Apr 2002 08:26:00 -0400
Subject: Re: too many aluminum stubs

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Hi,

I am sure you know how to remove silver paint and your samples from the stubs.
These tabs are carbon based and carbon burns in a muffle furnace at about
550º C.
When done, smear POL polish on a poly-jean or twill cloth and polish the
cylindrical stubs if you are worried about oxides and conductivity.

If you are using Al pin mounts, just throw them out. Your time cleaning
them costs more than a new bag of 100 mounts.

Paul



} This is almost embarrassing to ask, but I run a teaching lab for
} undergraduate students and we have accumulated literally hundreds of
} stubs with double sticky carbon mounts. I would love to clean them up
} and start all over, but the prospect of scraping the tapes off with a
} razor blade is daunting. Can anyone suggest a solvent or alternate
} procedure? Thanks in advance.
}
}
}



From daemon Sun Apr 14 12:56:00 2002



From: Alan E. Davis :      adavis-at-saipan.com
Date: Mon, 15 Apr 2002 09:54:14 +1000
Subject: Candle wax for Paraffin embedding?

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} From the material point of view, it almost costs you nothing. All you need
are some acetone and a few tea spoon of Sparkleen, which I believe most EM
lab has them.
Ping

--
Ping Li, Ph.D.
Director, Scientific Imaging Suite
Department of Biology
Dalhousie University
Halifax, NS B3H 4J1
Canada
Tel: 902-494-3309
Fax: 902-494-3736
E-mail: Ping.Li-at-Dal.Ca



-----Original Message-----
} From: John Hoffpauir [mailto:John.Hoffpauir-at-mail.tju.edu]
Sent: Sunday, April 14, 2002 12:35 PM
To: pli-at-is.dal.ca
Cc: microscopy-at-sparc5.microscopy.com; william.j.perreault-at-lawrence.edu


Is it possible, desireable, undesireable, to use candle paraffin (from the corner market) for embedding in a pinch? What would be the tradeoffs?

I'm sorry for the obviously off-mainstream request.

Alan Davis


--
Alan E. Davis, Science Instructor
Marianas High School
PMB 30, Box 10006,
Saipan, MP 96950
Northern Mariana Islands
adavis-at-saipan.com


"An inviscid theory of flow renders the screw useless, but the need
for one non-existent."
---Lord Raleigh(aka John William Strutt),or else
his son, Jr., who was also a scientist.


From daemon Sun Apr 14 20:37:40 2002



From: Ping Li :      pli-at-is.dal.ca
Date: Sun, 14 Apr 2002 22:26:48 -0300
Subject: Re: Clean aluminum stubs

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Further to my last message, for cleaning the stubs, I always use the acetone
collected from sample dehydration and EM mechanical part cleaning. So, the
recycled acetone probably should not be counted as a part of cost for
cleaning the stubs.

Although the cleaning process may take several hours, the time one really
involved in the work (a few minutes here and there) is only about half an
hour (for cleaning about 150 stubs in my lab). People can always do some
other work while the stubs are being soaking and sonicating.
Ping

}
} } From the material point of view, it almost costs you nothing. All you
need
} are some acetone and a few tea spoon of Sparkleen, which I believe most EM
} lab has them.
} Ping
}
} --
} Ping Li, Ph.D.
} Director, Scientific Imaging Suite
} Department of Biology
} Dalhousie University
} Halifax, NS B3H 4J1
} Canada
} Tel: 902-494-3309
} Fax: 902-494-3736
} E-mail: Ping.Li-at-Dal.Ca
}
}
}
} -----Original Message-----
} } From: John Hoffpauir [mailto:John.Hoffpauir-at-mail.tju.edu]
} Sent: Sunday, April 14, 2002 12:35 PM
} To: pli-at-is.dal.ca
} Cc: microscopy-at-sparc5.microscopy.com; william.j.perreault-at-lawrence.edu
} Subject: RE: Clean aluminum stubs
}
}
} is this method cost effective?
}
}
}



From daemon Sun Apr 14 20:46:32 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Sun, 14 Apr 2002 21:41:25 -0400
Subject: RE: Kodak 4127 Film

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Hi Steve,
I have always used Kodak 2415. Great for everything, and probably
worth a try on SEM. URL:
http://www.kodak.com/global/en/professional/support/techPubs/p255/p255.pdf

SPI recommends Kodak 4127 for SEM:
http://www.2spi.com/catalog/photo/kdakflm.shtml (Kodak URL: could NOT
find????)

Regards,

Fred Monson

} ----------
} From: Steve Beck
} Sent: Friday, April 12, 2002 2:04 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Kodak 4127 Film
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
}
} I have been using Kodak Professional film (4127) for many years in my SEM
} course. I have recently been informed that this film has been discontinued
} by Kodak. Does anyone know of a good replacement for this film?
}
} Thanks for your advice!
}
} Steve
}
} Stephen J. Beck
} Associate Professor
} Bio-Imaging Center/Electron Microscopy
} Department of Biology
} Nassau Community College
} Garden City, NY 11530
} Voice Mail: (516) 572-7829
} Email: {becks-at-sunynassau.edu}
} URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
}
}
}
}


From daemon Sun Apr 14 23:02:10 2002



From: Materials Research Laboratories, Inc. :      mrllab-at-raex.com
Date: Sun, 14 Apr 2002 23:51:11 -0400
Subject: Re: too many aluminum stubs

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on 4/14/02 8:26 AM, Beauregard at beaurega-at-westol.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I am sure you know how to remove silver paint and your samples from the stubs.
} These tabs are carbon based and carbon burns in a muffle furnace at about
} 550º C.
} When done, smear POL polish on a poly-jean or twill cloth and polish the
} cylindrical stubs if you are worried about oxides and conductivity.
}
} If you are using Al pin mounts, just throw them out. Your time cleaning
} them costs more than a new bag of 100 mounts.
}
} Paul
}
}
}
} } This is almost embarrassing to ask, but I run a teaching lab for
} } undergraduate students and we have accumulated literally hundreds of
} } stubs with double sticky carbon mounts. I would love to clean them up
} } and start all over, but the prospect of scraping the tapes off with a
} } razor blade is daunting. Can anyone suggest a solvent or alternate
} } procedure? Thanks in advance.
} }
} }
} }
}
}
}
I wrote to the requestor (individually) earlier but perhaps I should have
done the reply to all on the list.

The answer is simple: Soak the mounts in acetone and ultrasonically clean
for about an hour. The bath will soften any gunk, at which time the samples
can be rinsed in water and only a gentle (finger) abrasion is needed (you
can wear gloves for this if desired). If the solvent is especially dirty,
try another rinse or 2 - even with DI water a few times or a combination of
water and (m)ethanol. Lay the samples on a paper towel and you're done.
The samples will be perfect. Note: For Al or Cu, be sure to really dry
them well to prevent oxidation.

As far as aluminum mounts go, (pin or otherwise), they are fairly expensive
and at the rate of (estimated) $15/hour (as quoted by one of our members)
for someone to polish in an effort to recycle is well worth it - the
polishing just to resurface is only 1-2 minutes per mount. Easily, 20-30
can be cleaned within an hour by even an inexperienced technician. The
mounts cost substantially more than this to order new.

The amount of acetone needed is so miniscule that an environmental concern
for this is small relative to the idea of literally tossing away so much
metal.

I have had experience cleaning and resurfacing mounts of all types since the
early seventies. I see it as simply a peripheral aspect of an operator's
job. Obviously, if many have accumulated - it can seem overwhelming. But
small groups can be managed until all are done.

If you are concerned about cross-contamination, certainly never place a
sample (ie, a dispersion) directly onto the cleaned surface(s) without
verifying the integrity of your cleaning method. Having said that, I've
ordered 'new' mounts only to find residues from the final polish, so even
your own cleaning can be done quite well and often better than the
suppliers'.

The use of carbon tape or paint will generally provide enough of a clean
substrate for most other (non-dispersed) specimens.

Since EDS labs now typically all have detectors which 'see' carbon, even a
carbon mount sold as 'spectroscopically pure' is outdated. A simple diamond
polish for any carbon mount will return the (mount) to that state. One will
always have be cautious about contamination of any kind - including (now) C
if that is what you need to include in the analysis.

In closing, I would recommend random analyses of newly-purchased mounts to
ensure that indeed the surfaces are what is specified.


Carol Jean Hirt
Vice President, Materials Research Laboratories, Inc.
mrllab.com




From daemon Mon Apr 15 08:52:37 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 15 Apr 2002 08:40:28 -0500
Subject: RE: too many aluminum stubs

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I agree with Soumitra on this. I just can't get into the "use it and toss it" mode. It's a rare day that our student lab assistant doesn't have some spare time on her hands and, in addition, cleaning stubs could easily be one of the techniques taught to students, so they could recycle their own supply.

As for cost-effectiveness, I guess it depends upon circumstances and the nature of the lab's mission. It's partially a philosophical question of how we approach resources and could be debated ad infinitum. We will continue to reuse our stubs here.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/






John,

"Throwing them out" sounds like the most unreasonable idea. Majority of
the university EM labs run on a very low budget and just like Randy and
probably many other people we regularly recycle all our aluminum stubs and
many other items in the lab. I think people should try their best to
recycle items that can be recycled and used again. Aluminum stubs
certainly fall in that category. If someone still prefers to throw them
out, then he/she should check with the organization safety office and they
may come up with a solution to have them recycled.

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml

On Fri, 12 Apr 2002, John Hoffpauir wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} have you considered just throwing them out?
}
}





From daemon Mon Apr 15 08:54:44 2002



From: George Laing :      scisales-at-ngscorp.com
Date: Mon, 15 Apr 2002 09:48:16 -0400
Subject: RE: digital cameras again

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} Is there any difference between the same resolution cameras?
} The most used looks to me is the Nikon Coolpix 950... isn't } it? Anyone
experimented for example with Kodak 4900?

The Coolpix 950 had a 2.1 million pixel(MP) sensor and has been
discontinued for some time now. It's replacement, the Coolpix 995 which has
a 3.3MP sensor is a better comparison to the DC4900.
The 995 is similar in body style to the 950 with a number of improvements
including better noise reduction, contrast (saturation) control,
rechargeable battery (vs AA's) and a 4x optical zoom (vs 3x). An electronic
release is also available for the 995 which can reduce camera shake. Recent
price changes have made the 995 very affordable. The same microscope
couplers are used for both the 950 and 995 cameras.

I do not have experience with the Kodak 4900 for microscope use but in
general Kodak cameras produce high quality images. The DC4900 is a 4MP
camera with a 2x optical/ 3x digital zoom. I would check availability of
adapters for the DC4900, it does not have threads on the front of the lens.
The Canon G2 is also a 4MP camera, and the Nikon Coolpix 5000 is a 5MP
camera. Both have excellent image quality and adapters are available.

George

George Laing
National Graphic Supply
v:(800) 223-7130 x3109
f:(800) 832-2205
email: scisales-at-ngscorp.com



From daemon Mon Apr 15 08:57:27 2002



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Mon, 15 Apr 2002 09:51:42 -0400
Subject: Re: Kodak 4127 Film

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Hi Steve,
We have used Kodak Tmax 100 with good results when developed with Tmax
developer. You can continue to use your established brightness and
contrast settings if you change the f stop on your camera.
Frank


At 02:04 PM 4/12/02 -0400, Steve Beck wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Apr 15 09:11:43 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 15 Apr 2002 09:50:34 -0400
Subject: goof-off not goop-off

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Last week I mentioned that there was a good product on the market that took off adhesive material better than acetone. The product name was "Goof-Off" from Guardian and it contains xylene.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)




From daemon Mon Apr 15 09:39:51 2002



From: Margaret E. Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Mon, 15 Apr 2002 10:31:40 -0400
Subject: NESM's 19th Annual Woods Hole Symposium

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To all:

NESM's 19th Annual Woods Hole Symposium will be held May10-11th at Woods
Hole, MA.

This year, NESM is also hosting a Digital Imaging Short Course on May 9th
at Woods Hole.

The registration deadline for the course is April 19th and for the meeting
(with dinner Friday night) by May 3rd.

For details on this meeting and course, please go to NESM's
website: http://prism.mit.edu:8083 or the local affiliates page on the MSA
website: http://msa.microscopy.com/MSALAS/NESM/NESMHome.htm
The information will be in our April 2002 newsletter under "Current
Newsletter".

Peggy Sherwood
Corresponding Secretary, NESM
(New England Society for Microscopy)



From daemon Mon Apr 15 10:32:31 2002



From: Martin Goldberg :      MGoldberg-at-picr.man.ac.uk
Date: Mon, 15 Apr 2002 16:29:54 +0100
Subject: epitope tags for immuno-EM

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Dear List

I have just joined the list and this is my first question so I hope it is
appropriate. We are expressing recombinant proteins in Xenopus oocytes by
injecting the coding mRNA. We would like to locate the recombinant protein
by tagging the gene with a antigenic epitope such as myc, His, FLAG, Xpress
or maybe GFP and then use immuno-gold labelling both in thin cryo-sections
for the TEM and in field emission SEM. Does anyone have any experience of
which would be the best tag to choose for electron microscopy? And following
on from that what would be the best source (commercial?) of a suitable
antibody that would work well after aldehyde fixation? The cloning, etc. is
quite a lot of work so we don't really want to have to try too many
different tags - so any advice would be very gratefully received.

Martin

Dr. Martin Goldberg
CRC Dept. of Structural Cell Biology
Paterson Institute for Cancer Research
Christie Hospital
Wilmslow Road
Manchester
M20 9BX
UK

Tel 0161 446 3117
Fax 0161 446 3109
E-mail mgoldberg-at-picr.man.ac.uk {mailto:mgoldberg-at-picr.man.ac.uk}

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author and do not necessarily represent
those of the Paterson Institute For Cancer Research or the Christie Hospital
NHS Trust. It may contain information that is privileged & confidential
within the meaning of applicable law. Accordingly any dissemination,
distribution, copying, or other use of this message, or any of its contents,
by any person other than the intended recipient may constitute a breach of
civil or criminal law and is strictly prohibited. If you are NOT the
intended recipient please contact the sender and dispose of this e-mail as
soon as possible.


From daemon Mon Apr 15 11:26:26 2002



From: Christine Richardson :      a.c.richardson-at-durham.ac.uk
Date: Mon, 15 Apr 2002 17:17:37 +0100
Subject: TEM-Plunge freezing in liquid propane

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Dear All,
I have a member of staff who wants to try plunge freezing into liquid
Propane (pollen tubes).I have had lots of experience in the past with
isopentane and freon but I imagine it is whole new ball game with liquid
propane.My main concern is the safety aspect.Can anyone give me advice on
what equipment and procedure they use etc.
Has anyone done a risk assessment they are willing to share?
Is this a suitable technique to try in a general histo/e.m facility?
Where can I get more safety information?
For example some one pointed out that the fume cupboard should be spark
free?

Thanks in advance,
Chris.




From daemon Mon Apr 15 12:51:51 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Mon, 15 Apr 2002 13:43:59 -0400
Subject: Wrinkled thicks

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Hi Listers,

We are going nuts over here with wrinkles in our thick sections. We have tried coated (silane) and uncoated slides, the Super Frost Plus slides from Fisher. We have tried decreasing the temperature of the hot plate, we even bought a slide warmer for better consistancy with temp.

We are embedding a variety of human tissue samples and cell pellets using Spurr's. We can't think of what might have changed to cause these wrinkles.

Any ideas out there about how to get rid of wrinkles?

I'm getting more wrinkles (and gray hairs) tying to figure it out.


Help!


Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Mon Apr 15 12:54:48 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 15 Apr 2002 10:55:59 -0700
Subject: Re: digital cameras again

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There are numerous differences between Coolpix
models and other units with the same resolution.
Construction, adaptation, color balance, luminance
control, and "real resolution" are just a few of them.
One of the biggest problems with consumer cameras
is the effect of anti-aliasing. The CP950 has a
published rez of 2.1M pixels while the CP990 and 995
are 3.3M pixels. But these are not real resolutions.
The final resolution suffers from anti-aliasing.
The 950, 990, 995 and 5000 use a USB remote cable release
puck which is very handy for microscope use.
Also, the AC adapter is quite useful. Rechargeable
NiMh AAs are very good for use in the 950 and 990.
But the AC adapter makes this a moot issue. The
5000 uses Li-Ion module.

The 900-series use a fixed lens whereas the 5000's
lens protrudes from the front when turned on.
The threaded lenses of the 900s are easy to
adapt. I have not seen a scope adapter for the
5000, but suspect that one would exist.

I hope the following is not redundant....
This is a nicely done discussion about Coolpixes
and anti-aliasing by user nghy taken from digital photo usenet:

} Hello,
}
} I have been working extensively with the Coolpix 990. In general the
} camera itself works well. As far as I know the two models differ in
} that the 995 uses the newer and thicker compact flash memory
} cards/mini disk memory and the flash unit pops up and away from the
} camera body to prevent red eyd in portraits. There may be other minor
} differences as well but for use at the microscope they are
} functionally the same. The Coolpix is able to close fcus without
} other lenes and is capable of some macro work with out additional
} optics which are also available.
}
} The main problems with consumer cameras are 1) they use an
} anti-aliasing routine to "soften" the image to prevent some odd pixel
} effects and 2). the CCD chips are not physically cooled to limit the
} thermal noise which in turn reduces the dynamic range of the images.
} The design of the camera is not easily altered to circumvent either
} of these issues. The best you can do is to use photo editing software
} to massage and sharpen the image.
}
} Paranthetically, here is some information on anti-aliasing filters.
} http://www.kodak.com/global/en/professional/products/cameras/dcsTech/antiAliasingFilter/antiAliasing.jhtml
}
} There is no clear-cut best coupler.
}
} High end photographic systems designed for use with a microscope use a
} specially designed projection lens (in place of an eyepiece) to cast
} the image on the film. Camera backs are used without other
} photographic lenses.
}
} The 990 and 995 do not have removable lenses which means an eyepiece
} of one sort or another is required to relay the image to the camera
} lens. ( In this circumstance, the camera lens is set to focus at
} infinity and the aperture is set at its widest. The image is focused
} with the microscope controls and should be parfocal with the image at
} the eyepieces. The only problem will be with your own eyesight. The
} camera will not focus at the same point as your eyes so you will need
} to use your glasses to focus unless you develope a standard correction
} to compensate for the difference.)
}
} Herein lies the rub. Each of the microscope manufacturers builds into
} their eyepieces compensation for residual uncorrected abberitions in
} their objectives. These corrections are unique for the manufacturer
} and for the human eye which is the normal primary detector.
}
} There are adapters that are designed to connect the Coolpix camera to
} whatever eyepiece is designed for the microscope. Theorectically this
} is the best solution but in practice these may or may not work
} depending on the eyepiece design. My Zeiss widefield high eyepoint
} eyepieces vignette severly when using this type of adapter. This type
} adapter has not provided adequate results for me.
}
} So I must use "another" eyepiece. I have used a Leitz Periplan
} eyepiece that happily screws onto the Coolpix and found that this
} pretty much works but not to perfection. I have also used another
} eyepiece adapter designed by Optem specificaly for use with the
} Coolpix. Here again the results are different from the Leitz
} eyepiece but the conclusion is the same, pretty good but not perfect.
}
} The point is that the digital images can be striking and quite
} appealing IF you have never seen the same image at the microscope.
} The best images I have ever captured are a 6 or 7 on a scale of
} 1(worst) to 10 (best) when compared to the vue at the scope. This is
} frustrating and I am still searching for an answer.
}
} Therefore, I have made a compromise because the costs of a camera that
} comes closer to perfection is going to cost between 3 and 8 times as
} much as the Coolpix..

If you need high quality images, you need a high quality
camera. Not a consumer one. But the cost difference
is significant. It really depends on how much quality
you need and/or can afford.

gary g.



At 02:09 PM 4/12/2002, you wrote:

} Is there any difference between the same resolution cameras?
}
} The most used looks to me is the Nikon Coolpix 950... isn't it? Anyone
} experimented for example with Kodak 4900?
}
} Keep care and be of good cheer
}
} Regards
}
} (name) Vratislav Richard Eugene Maria John Baptist
} (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
} (title) 84 duke of Siebenlügner
}
} websites:
} http://www.coleoptera.org. and
} http://www.egroups.com/group/coleoptera
}
} University of Sydney
} The Wentworth Bldg., B 62
} NSW 2006
} AUSTRALIA
} phone : +61 414 540 465
} email: vratislav-at-bigfoot.com
} ICQ: 13610107
}
} Only after the last tree has been cut down,
} only after the last river has been poisoned,
} only after the last fish has been caught,
} only then will you find that money can not be eaten.'
} CREE INDIAN PROPHECY.
}
} Incoming mail is certified Virus Free.
} Checked by AVG anti-virus system (http://www.grisoft.com).



From daemon Mon Apr 15 13:13:49 2002



From: David H. Hall :      hall-at-aecom.yu.edu
Date: Mon, 15 Apr 2002 14:04:43 -0400
Subject: Re: epitope tags for immuno-EM

Contents Retrieved from Microscopy Listserver Archives
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Dear Martin,

We have published a post-embedding protocol for labelling plastic sections
using several commercial antibodies against GFP. We also have had success
with similar protocols using commercial antibodies against an HA tag
(unpublished). I would expect that you can bring the same commercial
antibodies to bear on cryosections too. We've generally found that success
at immunofluorescence is pretty good at predicting success or failure for
postembedding protocols. If the epitope survives strong fixes [or alcohol
washes] for immunofluorecence, then you can use that fix for immunoEM.

see:
Paupard, M.-C., Miller, A., Grant, B., Hirsh, D. and Hall, D.H. (2001)
Immuno-EM localization of GFP-tagged yolk proteins in C. elegans using
microwave fixation. J. Histochem. Cytochem. 49: 1-8.

There have still been problems for low abundance signals though. I can't
predict whether the mRNA construct will be of high enough abundance to
label above background. Good luck.

Dave
David H. Hall
Center for C. elegans Anatomy
Department of Neuroscience
1410 Pelham Parkway
Albert Einstein College of Medicine
Bronx, NY 10461

phone (718) 430-2195 FAX (718) 430-8821
hall-at-aecom.yu.edu
websites: www.aecom.yu.edu/wormem
www.wormatlas.org


From daemon Mon Apr 15 13:32:37 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 15 Apr 2002 12:30:32 -0600
Subject: RE: digital cameras again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been watching this thread for a while, and there are a few things
that nobody has mentioned so far (or I missed them) and which may be
important when it comes to decide about a camera:

1) Resolution
We often talk to people who think the more pixel the camera has the better
it is. That may apply to snapshots, but for microscopy there are other
considerations. For example, if you take pictures at the highest
magnification of the microscope, the resolution may be limited by the
microscope optics, not the resolution of the camera. All the millions of
pixels do is to provide empty resolution. In other words, the number of
pixels of the camera becomes more important, the LOWER the magnification of
the microscope becomes.

2) Sensitivity
For most cameras, the size of the chip is predetermined by some standards.
Doubling the resolution of the camera is accomplished by reducing the pixel
size. That automatically reduces the sensitivity per pixel, and in many
cases the overall sensitivity as well. In general, lower resolution cameras
have a higher sensitivity. B/W cameras are more sensitive than color
cameras.

3) Dark noise
Each camera produces dark noise, i.e., charge carriers are generated in the
CCD cells even when the chip is completely dark. The carriers are thermally
generated, and they accumulate during the exposure time. To reduce the dark
noise, many cameras are cooled. Astronomical cameras, for example, with
exposure times of hours, can be cooled to LN2. That's usually not necessary
for microscope cameras, but even a little cooling goes a long way, as the
thermal carrier generation grows exponentially with temperature.

4) live imaging
In our experience, a live preview of the image in full resolution helps the
microcopist tremendously. Not only allows it focusing on the screen, but in
many cases certain software features can be used for the live image
(auto-gain display, background subtraction, etc.), which can show features
that are not visible through the eye-pieces, or which would require intense
illumination, possibly destroying the sample (for example bleaching in
fluorescence).

5) Compression
Many "Snapshot" cameras acquire the images and immediately compress them
using JPG to save memory. For snapshots again that probably makes no
difference, but JPG is a lossy compression and leads to artifacts. We have
seen occasions, where the artifacts made it impossible to analyze the
images. That is not generally the case, but if you are looking for subtle
effects, stay away from JPG.

That's just my 2 cents worth of comments ....

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: George Laing [mailto:scisales-at-ngscorp.com]
Sent: Monday, April 15, 2002 7:48 AM
To: microscopy-at-sparc5.microscopy.com



} Is there any difference between the same resolution cameras?
} The most used looks to me is the Nikon Coolpix 950... isn't } it? Anyone
experimented for example with Kodak 4900?

The Coolpix 950 had a 2.1 million pixel(MP) sensor and has been
discontinued for some time now. It's replacement, the Coolpix 995 which has
a 3.3MP sensor is a better comparison to the DC4900.
The 995 is similar in body style to the 950 with a number of
improvements
including better noise reduction, contrast (saturation) control,
rechargeable battery (vs AA's) and a 4x optical zoom (vs 3x). An electronic
release is also available for the 995 which can reduce camera shake. Recent
price changes have made the 995 very affordable. The same microscope
couplers are used for both the 950 and 995 cameras.

I do not have experience with the Kodak 4900 for microscope use but
in
general Kodak cameras produce high quality images. The DC4900 is a 4MP
camera with a 2x optical/ 3x digital zoom. I would check availability of
adapters for the DC4900, it does not have threads on the front of the lens.
The Canon G2 is also a 4MP camera, and the Nikon Coolpix 5000 is a
5MP
camera. Both have excellent image quality and adapters are available.

George

George Laing
National Graphic Supply
v:(800) 223-7130 x3109
f:(800) 832-2205
email: scisales-at-ngscorp.com



From daemon Mon Apr 15 14:12:50 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Mon, 15 Apr 2002 15:06:36 -0400
Subject: Re: Imagining of Plakton

Contents Retrieved from Microscopy Listserver Archives
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have you consisdered using a scanning electron microscope?


From daemon Mon Apr 15 14:12:50 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Mon, 15 Apr 2002 15:04:17 -0400
Subject: Candle wax for Paraffin embedding?

Contents Retrieved from Microscopy Listserver Archives
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this one is filled with entanders, i probly misspelled that but couldn't
resist the temptation.


From daemon Mon Apr 15 15:31:10 2002



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Mon, 15 Apr 2002 15:25:58 -0500
Subject: RE: Wrinkled thicks and relief

Contents Retrieved from Microscopy Listserver Archives
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Hi Paula,
First of all, let me say that I know your pain, and most people have been
through this problem at one time or another, but it can be controlled with a
few tricks.

OK, first of all, to make your life easier, make sure that you trim away as
much excess plastic around the tissue as you can before you start cutting.
[with a razor blade] Uncoated slides are fine, but make sure that you let
them cook long enough on the hot plate or else they might come off when you
stain them. When we are warming our sections is the time that we type the
slide labels, so that they get a few extra minutes to stick to the slides.

You can cheat a bit and cut thicker than half a micron, say maybe 0.6 or 0.7
microns. They look just as good, but are a bit easier to cut.

For nerve and muscle, it has more of a tendency to wrinkle, so before I put
the sections on the hot plate, I force them to flatten out by holding a heat
pen over the sections. They will try to resist you, so you have to just
keep holding it and watching it until you darn well SEE the sections flatten
out before your very eyes. THEN is the time to put those babies onto the
hot plate.

We keep our hot plate at a temperature just too hot to touch without burning
yourself. I suppose if you hit the plate quickly with your hands it
wouldn't burn you. [1 notch below 2 on our particular hot plate, but I'm
not sure what the proper temp would be for you.

Finally, to move my sections from the knife boat to the slide [with a few
drops of water on it], I use a wooden stick that has a single cut across the
end at a 45 degree angle. This will assure you of an absolutely clean tool,
and also that freshly cut face will pick the sections up without folding
them.

Hope this helps,
Garry Burgess
Charge Technologist
Electron Microscopy
Health Science Centre
Winnipeg, Canada

Phone: 204-787-1508
FAX: 204-787-2381


Hi Listers,

We are going nuts over here with wrinkles in our thick sections. We have
tried coated (silane) and uncoated slides, the Super Frost Plus slides from
Fisher. We have tried decreasing the temperature of the hot plate, we even
bought a slide warmer for better consistancy with temp.

We are embedding a variety of human tissue samples and cell pellets using
Spurr's. We can't think of what might have changed to cause these wrinkles.

Any ideas out there about how to get rid of wrinkles?

I'm getting more wrinkles (and gray hairs) tying to figure it out.


Help!


Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Mon Apr 15 16:15:23 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 15 Apr 2002 16:09:06 -0500
Subject: Re: TEM-Plunge freezing in liquid propane

Contents Retrieved from Microscopy Listserver Archives
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Chris,

Plunge freeze into slush nitrogen instead. It's colder, there's no
Leidenfrost effect, and safe. No flammable cryogens condensing oxygen
out of the air, making for an even more explosive mixture.
To make slush nitrogen:
Fill a 600mL or 1000mL beaker with LN2, place in a vacuum desiccator,
attach to a rotary pump, of the size used for most sputter coaters or
larger.
Pump away. In a few minutes, you'll see the surface of the LN2
freezing over, then breaking up as a bubble of N2 surges through.
Keep pumping. Soon, there will be a mix of liquid & solid N2. Stop.
Pull out, place on an insulting pad, and plunge-freeze the samples,
dropping them into a container previously placed in the LN2.
Plunge-freeze until all samples are done (the LN2 is warming up).
When done freezing, then transfer the samples to whatever your next
step is.

Phil
P.S. Make sure the outlet in the desiccator collar is aligned with
the hole in the part of the desiccator lid over which the collar
fits. Otherwise you'll wonder -- as I did, one insufficiently
coffee'd Monday morning -- why the lid of the desiccator keeps
bumping, and the LN2 doesn't freeze.

} Dear All,
} I have a member of staff who wants to try plunge freezing into liquid
} Propane (pollen tubes).I have had lots of experience in the past with
} isopentane and freon but I imagine it is whole new ball game with liquid
} propane.My main concern is the safety aspect.Can anyone give me advice on
} what equipment and procedure they use etc.
} Has anyone done a risk assessment they are willing to share?
} Is this a suitable technique to try in a general histo/e.m facility?
} Where can I get more safety information?
} For example some one pointed out that the fume cupboard should be spark
} free?
}
} Thanks in advance,
} Chris.

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Mon Apr 15 16:23:35 2002



From: Arrowood, Roy :      arrowood-at-utep.edu
Date: Mon, 15 Apr 2002 15:12:33 -0600
Subject: candle wax for embedding

Contents Retrieved from Microscopy Listserver Archives
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Alan, I'm by no means an expert on paraffin embedment, or on microtomy in
general. So devalue my comments acordingly. However, when I was a
grade-school student doing amateur microscopy at home, I did have some
success with candle wax embedment of plant parts. It was hardly
professional-grade work-- my microtome consisted of a bolt, a nut, and a
honed razor blade. But I was able to do some fairly decent sections (at
least for my purposes). I would hesitate to entrust irreplaceable research
specimens to candle wax today. However, if you want to mount commonplace
specimens for educational purposes, candle wax might be worth a try.

Keep in mind that candle wax formulas are rather variable, and not optimized
for the microscopist. Candlemakers often add vegetable oil to the wax,
which does help to make it less brittle than unmodified paraffin. (It may
also be less likely to bond well enough to your sample, however.) I vaguely
remember that as a kid I experimented with further additions of oil (and
also beeswax, pine resin, etc) to candle wax. Usually, however, I think I
just embedded things in a particular brand of candle wax. without adding
anything to improve the wax. But many a year has gone by, and my memory may
not be reliable on that point.

Good luck!

-------Roy
=================================================
Roy Arrowood arrowood-at-utep.edu
Metallurgical and Materials Engineering
University of Texas at El Paso
El Paso, TX 79968-0520
(915)747-6934 voice // (915)747-8036 FAX
(915)747-5468 secretary






From daemon Mon Apr 15 19:39:00 2002



From: John Foust :      jfoust-at-threedee.com
Date: Mon, 15 Apr 2002 19:25:43 -0500
Subject: RE: digital cameras again

Contents Retrieved from Microscopy Listserver Archives
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Has anyone mentioned color? In a non-microscopy realm,
I've had maddening results from an Olympus C-2500L, a
well-rated camera from about 18 months ago. It's nearly
impossible to predict the fine details of its color
reproduction.

Although you can set the white balance to a new target
under a given light, or leave it on automatic, there's
no way to stop it from attempting to color-balance.
It looks at the scene, attempts to find "white" and
"black" and uses the contents of the scene to tweak
the colors.

In controlled situations such as an object with a single
subtle range of colors (in my case, four square ceramic
tiles of slightly varied colors) on a black velvet background,
it comes up with different colors when I also place other
colored objects in the scene, or when there's a pure white
in the scene, etc.

- John



From daemon Mon Apr 15 19:47:06 2002



From: Earl Weltmer :      earlw-at-sbcglobal.net
Date: Mon, 15 Apr 2002 17:39:17 -0700
Subject: JEOL S-880 parts

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

I have some JEOL S-880 parts (holders, etc.) free to anyone who wants them.

This is a JEOL S-880 not the S-840 series.
The S-880 was an immersion lens SEM.

Thank You,

Earl Weltmer
(714) 573-9158




From daemon Mon Apr 15 21:41:01 2002



From: Jay.Jerome-at-mcmail.vanderbilt.edu (by way of MicroscopyListserver)
Date: Mon, 15 Apr 2002 21:30:03 -0500
Subject: New Premeeting Short Course for M&M '02

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Colleagues:

A new pre meeting short course has been added to the Microscopy &
Microanalysis 2002
conference offerings.

Title: "High pressure freezing plus freeze substitution equals a new
approach to immunolabelling".

Outline: Introduction to High Pressure Freezing, HPF. Why HPF?
Advantages of High Pressure Freezing
over conventional - chemical and/or microwave fixation. After HPF
what techniques/methods of tissue
examination can be used - cryosectioning, cryoplaning, freeze
substitution and/or immuno labeling.
Techniques and examples of Immuno Labelled HPF tissues and cells


Details on all M&M '02 short courses can be found on the Meeting WWW page.

http://www.microscopy.com/MSAMeetings/MMMeeting.html




-----------------------------------------------------------------------
. AKA: W. Gray Jerome, Ph.D., FAHA .
. Department of Pathology .
. Vanderbilt University Medical Center .
. B-4220 Medical Center North .
. 1161 21st Ave, South .
. Nashville, TN 37232-2561 .
. Phone: 615-322-5530 .
. Fax: 615-343-7023 .
. Email: jay.jerome-at-mcmail.vanderbilt.edu .
.......................................................................



From daemon Mon Apr 15 23:47:55 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Mon, 15 Apr 2002 21:37:47 -0400
Subject: Re: TEM-Plunge freezing in liquid propane

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on 4/15/02 12:17 PM, Christine Richardson at a.c.richardson-at-durham.ac.uk
wrote:

} I have a member of staff who wants to try plunge freezing into liquid
} Propane (pollen tubes).I have had lots of experience in the past with
} isopentane and freon but I imagine it is whole new ball game with liquid
} propane.My main concern is the safety aspect.Can anyone give me advice on
} what equipment and procedure they use etc.
} Has anyone done a risk assessment they are willing to share?
} Is this a suitable technique to try in a general histo/e.m facility?
} Where can I get more safety information?
} For example some one pointed out that the fume cupboard should be spark
} free?
}
Dear Chris,
If you do not chose to follow Phil's suggestion, here's how I
plunge-froze with propane:

First, get a room with very good air circulation--I used one with ~3
complete change-overs per hour--then gather everything you need. I used a
~5 ml metal bucket mounted on 3 prongs to hold it near the top of a LN
dewar, the dewar, the plunger and stand, tweezers (equipped with an o-ring
to hold them closed, the reverse ones didn't hold my sample grids), grids,
sample, filter paper, micropipette, and a bottle of propane with a low-flow
regulator and a tygon line with a pipette tip fixed to the end. When the
dewar and bucket have been mounted under the plunger and cooled to 77 K,
start the propane flowing into the bucket until you have liquid nearly to
the top. This requires practice to do; too little flow and the propane
freezes, too much and it never cools enough to liquify. Start sample
loading, blotting, and plunging. When the propane starts to freeze, add
more gas at a low flow rate, moving the pipette tip around the solid propane
to melt it. Continue until done. When done, put the bucket with the
propane into a hood. I've done this both in a cold room and a
(well-ventilated) lab. I have not heard of any problem for anyone doing
plunge-freezing this way (n=~15), but there is certainly a safety concern.
I don't know if the Electron Microscopy Safety Handbook covers this topic.
(My copy is presently unavailable.) I'd guess that the procedure is as safe
as using diethyl ether in a lab, so it should be OK in a histo/EM facility.
Good luck.
Yours,
Bill Tivol



From daemon Tue Apr 16 02:33:36 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Tue, 16 Apr 2002 09:24:51 +0200
Subject: Re: digital cameras again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A lot of people keep thinking that when a camera has more pixels, this
results in better pictures in microscopy. A very basic issue however is
the size of each individual pixel in relation to the resolution of the
microscope. When you do digital micrscopy it is very important to
understand the Nyquist sampling principle and in addition the relation
between the size of the object and the C.V. of the measurement derived
from the digital image on the CCD array. A very simple principle is that
you always have to start from analysing the resolution of your
microscope from the N.A. and that magnification is only relevant to
"project" the image on the spatial "grid" of the detection "system"
(human eye, camera, ...).

An excellent source of information about the use of digital cameras in
microscopy:
http://www.ph.tn.tudelft.nl/People/young/manuscripts/QM/QM.html

A very simple way to understand the relation between the size of a
CCD-element and its sensitivity is to see one CCD element as a "bucket"
for photons. The larger the CCD-element, the more photons it can hold to
build up its charge (lectrons) and the more "sensitive" the camera. But,
if you increase the CCD-element (xy-dimensions), you decrease its
spatial resolution. The larger the CCD-elements the more you have to
magnify the microscope image for a given resolution
(http://ourworld.compuserve.com/homepages/pvosta/pcrnyq.htm).

And last but not least the issue of compression. I agree that JPG
compression can be used in presentations and publication, but avoid it
when you need to analyse the images afterwards. We use JPG compression
for B/W images in certain situations, but never use it for color images.
In situations where we acquire more than 3x28000 images per day with our
automated microscope, we have to use compression ;-) An alternative for
JPG compression is to use LZW compression for TIFF images. In general
try to us lossless compression algorithms for quantitative microscopy.

This is my 1 eurocent worth of comments.

Best regards,

Peter

P.S. this is my personal opinion.

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)
Cipalstraat 3
B-2440 Geel
Belgium
tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

====================================================================
Mike Bode wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have been watching this thread for a while, and there are a few things
} that nobody has mentioned so far (or I missed them) and which may be
} important when it comes to decide about a camera:
}
} 1) Resolution
} We often talk to people who think the more pixel the camera has the better
} it is. That may apply to snapshots, but for microscopy there are other
} considerations. For example, if you take pictures at the highest
} magnification of the microscope, the resolution may be limited by the
} microscope optics, not the resolution of the camera. All the millions of
} pixels do is to provide empty resolution. In other words, the number of
} pixels of the camera becomes more important, the LOWER the magnification of
} the microscope becomes.
}
} 2) Sensitivity
} For most cameras, the size of the chip is predetermined by some standards.
} Doubling the resolution of the camera is accomplished by reducing the pixel
} size. That automatically reduces the sensitivity per pixel, and in many
} cases the overall sensitivity as well. In general, lower resolution cameras
} have a higher sensitivity. B/W cameras are more sensitive than color
} cameras.
}
} 3) Dark noise
} Each camera produces dark noise, i.e., charge carriers are generated in the
} CCD cells even when the chip is completely dark. The carriers are thermally
} generated, and they accumulate during the exposure time. To reduce the dark
} noise, many cameras are cooled. Astronomical cameras, for example, with
} exposure times of hours, can be cooled to LN2. That's usually not necessary
} for microscope cameras, but even a little cooling goes a long way, as the
} thermal carrier generation grows exponentially with temperature.
}
} 4) live imaging
} In our experience, a live preview of the image in full resolution helps the
} microcopist tremendously. Not only allows it focusing on the screen, but in
} many cases certain software features can be used for the live image
} (auto-gain display, background subtraction, etc.), which can show features
} that are not visible through the eye-pieces, or which would require intense
} illumination, possibly destroying the sample (for example bleaching in
} fluorescence).
}
} 5) Compression
} Many "Snapshot" cameras acquire the images and immediately compress them
} using JPG to save memory. For snapshots again that probably makes no
} difference, but JPG is a lossy compression and leads to artifacts. We have
} seen occasions, where the artifacts made it impossible to analyze the
} images. That is not generally the case, but if you are looking for subtle
} effects, stay away from JPG.
}
} That's just my 2 cents worth of comments ....
}
} mike
}
} } } } } } } } } } } WE HAVE MOVED { { { { { { { { {
} please make a note of the new address below
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
} -----Original Message-----
} } From: George Laing [mailto:scisales-at-ngscorp.com]
} Sent: Monday, April 15, 2002 7:48 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: RE: digital cameras again
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } Is there any difference between the same resolution cameras?
} } The most used looks to me is the Nikon Coolpix 950... isn't } it? Anyone
} experimented for example with Kodak 4900?
}
} The Coolpix 950 had a 2.1 million pixel(MP) sensor and has been
} discontinued for some time now. It's replacement, the Coolpix 995 which has
} a 3.3MP sensor is a better comparison to the DC4900.
} The 995 is similar in body style to the 950 with a number of
} improvements
} including better noise reduction, contrast (saturation) control,
} rechargeable battery (vs AA's) and a 4x optical zoom (vs 3x). An electronic
} release is also available for the 995 which can reduce camera shake. Recent
} price changes have made the 995 very affordable. The same microscope
} couplers are used for both the 950 and 995 cameras.
}
} I do not have experience with the Kodak 4900 for microscope use but
} in
} general Kodak cameras produce high quality images. The DC4900 is a 4MP
} camera with a 2x optical/ 3x digital zoom. I would check availability of
} adapters for the DC4900, it does not have threads on the front of the lens.
} The Canon G2 is also a 4MP camera, and the Nikon Coolpix 5000 is a
} 5MP
} camera. Both have excellent image quality and adapters are available.
}
} George
}
} George Laing
} National Graphic Supply
} v:(800) 223-7130 x3109
} f:(800) 832-2205
} email: scisales-at-ngscorp.com


From daemon Tue Apr 16 03:33:39 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Tue, 16 Apr 2002 09:29:07 +0100
Subject: Re: digital cameras again

Contents Retrieved from Microscopy Listserver Archives
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One of the shortcomings of traditional single-chip colour ccds (I
think all current compact digital cameras to date use single chips) is
that the three colours at each location are measured by
cc devices at three separate sample locations, which may have three
separate intensities and colour values. The colour values must be
estimated by interpolation within and between adjacent sensor groups,
and there is scope for error in this estimation. The use of
three-chip cameras is one way round this, using beam-splitters to
project the components of the image onto three separate monochrome
sensor arrays, the limitation here being that the image projection and
alignment must be perfect. Recently Foveon has introduced a
single-chip sensor, Foveon X3 in which for the first time three
colours are measured at different depths at the same pixel location, a
process which is the silicon equivalent of imaging with a colour film
emulsion. Foveon X3 is said to eliminate the colour estimation errors
associated with traditional ccds and to provide better spatial
resolution per pixel as well. Is it too early for list members to have
had hands-on experience of this very promising sensor?

Chris

Disclaimer: I have no financial interest in Foveon or their products
other than a sharp price when (if) I buy them.




From daemon Tue Apr 16 06:09:38 2002



From: Melina Meli :      applina-at-libero.it
Date: Tue, 16 Apr 2002 12:38:15 +0000
Subject: Gold Coating Composition

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Can anybody tell me what's the exact composition of gold or Gold/Palladium
coating? I.E. It is possible to find high P, Si, Fe, (much higher than Au L)
peaks in an X-ray spectrum using a Pantafet EDS detector with a LEO 440 SEM?
Thanks
Melina



From daemon Tue Apr 16 09:13:12 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 16 Apr 2002 09:01:24 -0500
Subject: RE: Gold Coating Composition

Contents Retrieved from Microscopy Listserver Archives
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I think your EDS detected substrate under Au coating -
this coating usually very thin and mostly transparent to
EDS analysis.

Something should go terribly wrong with your coater
(like completely worn down Au foil) if you have Fe
in coated layer. Try to check it by coating spectroscopically
pure carbon and analyzing with EDS.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

}
}
} Can anybody tell me what's the exact composition of gold or
} Gold/Palladium
} coating? I.E. It is possible to find high P, Si, Fe, (much
} higher than Au L)
} peaks in an X-ray spectrum using a Pantafet EDS detector with
} a LEO 440 SEM?
} Thanks
} Melina
}
}
}


From daemon Tue Apr 16 09:25:05 2002



From: Arey, Bruce W :      bruce.arey-at-pnl.gov
Date: Tue, 16 Apr 2002 07:16:46 -0700
Subject: Plug in for Stereology Calculations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking at doing some stereology on rodent lungs. Does anyone know
of a program that will impose a "computer generated cycloid grid containing
24 evenly spaced points and sine-wave cycloid lines over a digitized image."
Our Optimas program can acquire and digitize the image but I need some sort
of toolbox of items to place this grid over the image and have it calculate
how many times the object of interest crosses the cycloid line and how many
times it crosses the points. The paper cited refers to a Stereology Toolbox
TM software by Morphometrix, Davis, CA and I have been unable to locate it
in the phone book or on the internet. Suggestions??? Thank you in advance
for any light you can shed our way. I know how to use our Optimas program
but it does have limitations (many) and this just happens to be one of them.


Bruce W. Arey

PNNL-Battelle
Associate Scientist
Microstructural Characterization
bruce.arey-at-pnl.gov
509-376-3363
fax 509-376-6308




From daemon Tue Apr 16 09:57:30 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Tue, 16 Apr 2002 10:48:52 -0700
Subject: Re: TEM-Plunge freezing in liquid propane

Contents Retrieved from Microscopy Listserver Archives
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Hi Christine,
Read Stephenson JL. 1954. Caution in the use of liquid propane for freezing
biological specimens. Nature (London) 174. 235.
Zabetakis MG. 1967. Safety with Cryogenic Fluids. Plenum, NY.
I use extreme caution when using propane. It's always best to practice safe
science and have someone bless the freezing;-) Post signs on the door that
say "No spark or flame - Flammable material in use".
Work in the hood. Unplug all electrical devices from the outlets on the hood.
Use an air-driven stirrer. The stirrer shaft and blade may need to be
modified by a machine shop to fit the propane well/cup.
Don't push it! Limit your freezing time to 45 minutes - 1 hour. Then pour
the propane from the well/cup into a stainless steel bowl and let it
evaporate in the hood.
Also, read Howard and O'Donnell's paper (where the above references came
from). Freeze substitution of Fungi for Cytological Analysis. Experimental
Mycology 11, 250-269 (1987).
When it works (good freezing), the results are spectacular!
happy freezing,
Beth Richardson
PS - are we cousins?

} Dear All,
} I have a member of staff who wants to try plunge freezing into liquid
} Propane (pollen tubes).I have had lots of experience in the past with
} isopentane and freon but I imagine it is whole new ball game with liquid
} propane.My main concern is the safety aspect.Can anyone give me advice on
} what equipment and procedure they use etc.
} Has anyone done a risk assessment they are willing to share?
} Is this a suitable technique to try in a general histo/e.m facility?
} Where can I get more safety information?
} For example some one pointed out that the fume cupboard should be spark
} free?
}
} Thanks in advance,
} Chris.


**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Tue Apr 16 10:04:54 2002



From: Bennett-stamper, Christina (BENNETCN) :      BENNETCN-at-UCMAIL.UC.EDU
Date: Tue, 16 Apr 2002 10:58:54 -0400
Subject: RE: Imagining of Plankton

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I have not tried this in plankton but I have had cases where I have
needed overall fluorescence for various reasons. I think there may be a
couple of ways to do this.

You could fix the creature with something like Gluteraldehyde that is highly
fluorescent to see if there is enough fluorescence to measure.

You could try a more generally staining compound like phalloidin that stains
actin...which is usually all over an organism. It can be purchased with any
number of fluorophores attached then used quantify the fluorescence.

It wasn't stated how these were being imaged, I assume you are using a
confocal to do this. If you aren't, maybe you should try. Many confocal
scopes have software that will quantify fluorescence and may allow you to
calculate surface area. Additionally, I have seen a 3D/4D program called
Velocity put out by a company called Improvision (of which I have no stake
in) which will take confocal Z-stacks and create very nice 3D
reconstructions from which the surface area can be measured.

I hope this helps, feel free to contact me off line if you have any
questions. I have some other ideas but I would need to know more about the
creature.

Christina

~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~
Christina L. Bennett-Stamper
Operations Manager
Center for Biological Microscopy

University of Cincinnati
Vontz Center for Molecular Studies
3125 Eden Ave, P.O. Box 670521
Cincinnati, OH 45267-0521

cell: 513 703 0355
office: 513 558 6613
fax: 513 558 4454
~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~


From daemon Tue Apr 16 10:51:37 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 16 Apr 2002 09:18:52 -0400
Subject: RE: Candle wax for Paraffin embedding?

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The paraffin you get in the store is pure [you can use it to make candy!]!
Embedding paraffin used to be made up. Here are some old recipes from Gray,
P, "Microtomists Formulary and Guide", Krieger Publishing.

Altman: 60degreeMP: paraffin 85, tristearin 10, beeswax 5.
Beyer: paraffin 100, rubber 2, beeswax 0.5
Gray 1941: paraffin(MP 58) 70, rubber 5, beeswax 5, spermaceti 5,
nevillite "5" or clarite " 15 [see nailpolishes for this acrylic polymer]
NOTE: this composition melts at about 50 but will cut 5um ribbons at room
temp up to 85 degrees F.
Pohlman: paraffin 10, bayberry wax 1
Modern paraffin embedments are mixtures of paraffin and various
other components including polyethylene glycols of different MW's (I think!)

The above have to mixed thoroughly, and you must BELIEVE that there is NO
other way!

Regards,

Fred

} ----------
} From: Alan E. Davis
} Sent: Sunday, April 14, 2002 7:54 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Candle wax for Paraffin embedding?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is it possible, desireable, undesireable, to use candle paraffin (from the
} corner market) for embedding in a pinch? What would be the tradeoffs?
}
} I'm sorry for the obviously off-mainstream request.
}
} Alan Davis
}
}
} --
} Alan E. Davis, Science Instructor
} Marianas High School
} PMB 30, Box 10006,
} Saipan, MP 96950
} Northern Mariana Islands
} adavis-at-saipan.com
}
}
} "An inviscid theory of flow renders the screw useless, but the need
} for one non-existent."
} ---Lord Raleigh(aka John William Strutt),or else
} his son, Jr., who was also a scientist.
}
}


From daemon Tue Apr 16 11:09:35 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 16 Apr 2002 12:07:44 -0400
Subject: goof-off not goop-off

Contents Retrieved from Microscopy Listserver Archives
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To All;

Has anyone tried any terpine based solvents? I believe they are derivatives
of citrus fruits like lemons etc. and they seem to be used a great deal
printed circuit board cleaning and de-fluxing. It is also an
environmentally friendly material and may be disposed of in a common drain
with no treatment and it's non-flammable.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Monday, April 15, 2002 9:51 AM
To: Microscopy (E-mail)


Last week I mentioned that there was a good product on the market that took
off adhesive material better than acetone. The product name was "Goof-Off"
from Guardian and it contains xylene.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)




From daemon Tue Apr 16 11:17:23 2002



From: David John :      djohn-at-tcd.ie
Date: Tue, 16 Apr 2002 17:06:17 +0100
Subject: Running costs of FEG v. LaB6 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

We are considering the purchase of a high resolution transmission electron
microscope and I am interested in finding out the true costs of operating a
FEGTEM versus a LaB6 TEM. Does a FEGTEM cost appreciably more to operate
than a LaB6 and is the better resolution of the FEG it's only major
advantage?
I can obtain some information such as service contact costs from the
manufacturers but I would like to hear of the experiences of actual users.

Regards,
David John

Centre for Microscopy and Analysis,
Trinity College,
Dublin 2,
Ireland.

Tel. (353) - 1 - 6081559
Fax. (353) - 1 - 6770438




From daemon Tue Apr 16 11:37:46 2002



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Tue, 16 Apr 2002 12:31:16 -0400 (EDT)
Subject: thanks to all

Contents Retrieved from Microscopy Listserver Archives
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Hi listers,

Thanks to all the folks I've been in contact with about my used prep
equipment - I wish I could help out all the needy labs out there!

Dee



***************************************************************
Please do not publicly post any of my correspondence without permission

Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 845/365-8640
F: 845/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Tue Apr 16 11:51:33 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 16 Apr 2002 12:45:07 -0400
Subject: While looking for a CCD....

Contents Retrieved from Microscopy Listserver Archives
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I found an unused "Optronics TEC-470 with a 1/2" Interline Transfer
Hyper-HAD CCD, Complimentary Color Mosaic Filter" which I connected to a 14"
Electrohome RGB monitor for real-time viewing of material viewed on my
Ortholux I with my 25 and 50X Water immersion objectives.

Now, I wanted a CCD camera and was listening VERY closely to the discussion
of the Nikons, etc.

I now have the following specifications to deal with:


"Optronics TEC-470 with a 1/2" Interline Transfer Hyper-HAD CCD,
Complimentary Color Mosaic Filter"
"Minimum illumination: 0.0002 lux at f/1.2, 30 IRE output(4min + 0dB
gain)"
"Light range: 0.0002 lux to 13,000 lux"
"Signal to Noise Ratio: 53dB (50 IRE output, 0dB gain)"
"Shutter speeds: 0dB AGC gain: 1/10000 - 4min"(approx 2-fold
stops)
"Exposure range: 65,000,000:1 (156dB) measured from 30 IRE to
clipping"
"Cooled temperature: 37oC below ambient"
"Scanning system: NTSC: 2:1 Interlaced, 525 lines, 30 fps"
"Sensing Area: 6.4mm x 4.8mm (Equivalent to 1/2 inch optical
format"
"Optics: Integral adapting optics for 1" format with C-mount
thread"
"Auto White Balance: Range: 2000oK to 6500oK"
"Picture elements: NTSC: 768 x 494"

Without understanding half of what I have written, I think I have found a
solution that exceeds my needs, which is OK. The questions I have are
these.

1. Given the "Picture elements" and whatever else is relevant, in what
(kind of?) frame grabber should I invest to capture what this camera has to
offer.

2. Without having to pay one of you guys to come and tutor me, where can I
go for an explanation of the jargon I have displayed above so that I can
speak intelligently about the capabilities of my new/old toy.

Regards and thanks,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin/wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

From daemon Tue Apr 16 12:57:55 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Tue, 16 Apr 2002 12:53:15 -0500
Subject: Nikon DXM1200

Contents Retrieved from Microscopy Listserver Archives
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I've followed the thread on digital cameras and have decided to ask for
off-line opinions on the Nikon DXM1200 digital camera for attachment to
optical microscopes. I am not able to get as much information about the
camera's specifications as I would like. It is not cooled to the best of
my knowledge and it uses a rather unusual method of collecting an image,
pixel shift. A test yielded rather good images and compared favorably with
other cameras I've used, i.e better than others. The camera will be used
primarily for bright field color images but some polarized light, HMC and
DIC as well.

Damian Neuberger
Baxter Healthcare Corp
damian_neuberger-at-baxter.com or
neuberger1234-at-attbi.com




From daemon Tue Apr 16 13:17:47 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 16 Apr 2002 13:11:19 -0500
Subject: Re: Plug in for Stereology Calculations

Contents Retrieved from Microscopy Listserver Archives
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you could add it as a semi-transparent layer using Photoshop. To
generate the sine wave, you could use any graphing program to plot
out a sine wave and then copy it into Photoshop and erase the
extraneous info like the X & Y axi.

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Apr 16 13:21:30 2002



From: jerzy.gazda-at-amd.com
Date: Tue, 16 Apr 2002 13:15:29 -0500
Subject: Running costs of FEG v. LaB6 TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear David,
our lab operates two TEMs, JEOL2010 LaB6 (6years old) and FEI CM300FEG (4 years old). Both of them are operated 24-7-365 because we support production in the Fab. The service contract cost for the two tools are comparable as we get multiple-tool discount. I would recommend always to have a service contract in place.

The true cost to us is the down time. From my experience over the last three years, the JEOL runs 97% of the time, and CM ~80% (issues with cameras and attachments included). Our JEOL gets a new filament and set of apertures every six months with a PM. CM had it first tip exchanged after 4.5 years of service and it took a week to get it done. Our techs prefer to use the JEOL because of experience and ease of use.
Our senior analysts drive the CM because of analytical equipment and ease of use ;). Yes, we do not agree!

On CM it takes ~5 minutes to exchange a sample and obtain lattice image of Si(110) while it might be a two hour ordeal on 2010 on a good day. Both scopes are sitting side by side in the same room so the environment is comparable. Both use digital cameras.

Your decision will also depend on other factors, but if possible go with a FEG from whatever manufacturer you prefer! Hitachi makes a great FEGs too.

Disclaimer, the names of manufacturers are used for background setting purposes only, I am a very satisfied user of both systems and do not have any financial interest in any of the mentioned companies.


Jerzy
******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************



-----Original Message-----
} From: David John [mailto:djohn-at-tcd.ie]
Sent: Tuesday, April 16, 2002 11:06 AM
To: "Microscopy-at-MSA.Microscopy.Com"-at-tcd.ie


Dear All,

We are considering the purchase of a high resolution transmission electron
microscope and I am interested in finding out the true costs of operating a
FEGTEM versus a LaB6 TEM. Does a FEGTEM cost appreciably more to operate
than a LaB6 and is the better resolution of the FEG it's only major
advantage?
I can obtain some information such as service contact costs from the
manufacturers but I would like to hear of the experiences of actual users.

Regards,
David John

Centre for Microscopy and Analysis,
Trinity College,
Dublin 2,
Ireland.

Tel. (353) - 1 - 6081559
Fax. (353) - 1 - 6770438






From daemon Tue Apr 16 14:10:16 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Tue, 16 Apr 2002 14:59:28 -0400
Subject: Plug in for Stereology Calculations

Contents Retrieved from Microscopy Listserver Archives
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The book Unbiased Stereology by Howard and Reed lists Kinetic Imaging LTD
(http://www.kineticimaging.com) as a supplier of stereology software.
Everett Ramer
Cellomics, Inc.

-----Original Message-----
} From: Arey, Bruce W [mailto:bruce.arey-at-pnl.gov]
Sent: Tuesday, April 16, 2002 10:17 AM
To: 'Microscopy-at-MSA.Microscopy.com'


We are looking at doing some stereology on rodent lungs. Does anyone know
of a program that will impose a "computer generated cycloid grid containing
24 evenly spaced points and sine-wave cycloid lines over a digitized image."
Our Optimas program can acquire and digitize the image but I need some sort
of toolbox of items to place this grid over the image and have it calculate
how many times the object of interest crosses the cycloid line and how many
times it crosses the points. The paper cited refers to a Stereology Toolbox
TM software by Morphometrix, Davis, CA and I have been unable to locate it
in the phone book or on the internet. Suggestions??? Thank you in advance
for any light you can shed our way. I know how to use our Optimas program
but it does have limitations (many) and this just happens to be one of them.


Bruce W. Arey

PNNL-Battelle
Associate Scientist
Microstructural Characterization
bruce.arey-at-pnl.gov
509-376-3363
fax 509-376-6308




From daemon Tue Apr 16 14:55:17 2002



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Tue, 16 Apr 2002 15:48:58 -0400
Subject: Fwd: to ask about better SEM preparation for larvae sample

Contents Retrieved from Microscopy Listserver Archives
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}
} } Please reply direstly to this inquiry that came to me as I have not had
} } experience with this type of specimen.
} }
} } Sir,
} }
} } I am doing my masters project in University Science Malaysia, Malaysia
} } dealing with morphological studies of ants. I need to use SEM to
} } characterize the morphological features of different stages of ants in
} } order to differentiate them. I had tried the CPD method which can give
} } quite a good picture on later stage of larvae but not for early stages. I
} } had tried also freeze drying and 1,1,1,3,3,3 hexamethyldisilazan drying
} } method but also couldn't get consistant results. Most of the time I
} } couldn't get a smooth surface of fixed larvaes. It would be appreciated
} } if you can give me some advices about that. Thanks.
} }
} } Cheers,
} } Annie

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Ditrector, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/emcl


From daemon Tue Apr 16 15:16:28 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Tue, 16 Apr 2002 16:15:07 -0400
Subject: Re: goof-off not goop-off

Contents Retrieved from Microscopy Listserver Archives
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Peter,

The correct spelling of the class name is "terpene", in case anyone
intends to look it up, and they are certainly NOT non-flammable,
although they have higher flashpoints than some of the compounds that
they are replacing. Perhaps you have seen commercial formulations that
contain these solvents in combination with other agents. In this case,
there are some combinations that have very little flammability risk.

Some of the commercial preparations for stripping paint involve
citrus-derived terpenes and n-methylpyrrolidinone. There are many
others. Since the terpene constituents are not very water soluble, they
often include emulsification agents to assist in their dispersal in
rinse water.

Flammability remains something to be concerned about until the agent is
diluted. As a cautionary tale, many of the old citrus industry packing
houses met their end with catastrophic fires fueled by the "solvent" in
the fruit peels, a well aerated heap of grapefruit being a bit like
kerosene soaked green wood.

On the environmental side, one should note that while the defluxing
agent itself may be disposable without restriction, once used to de-flux
solder work done with lead-based solder, it will contain dissolved
lead-rosinate which most certainly cannot be disposed of in a "common
drain".

John Twilley

Peter Tomic wrote:

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} -----------------------------------------------------------------------.
}
}
} To All;
}
} Has anyone tried any terpine based solvents? I believe they are derivatives
} of citrus fruits like lemons etc. and they seem to be used a great deal
} printed circuit board cleaning and de-fluxing. It is also an
} environmentally friendly material and may be disposed of in a common drain
} with no treatment and it's non-flammable.
}
} Peter Tomic
} Anadigics, Inc.
}
} -----Original Message-----
}
} } From: Walck, Scott D. [mailto:walck-at-ppg.com]
}
} Sent: Monday, April 15, 2002 9:51 AM
} To: Microscopy (E-mail)
} Subject: goof-off not goop-off
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Last week I mentioned that there was a good product on the market that took
} off adhesive material better than acetone. The product name was "Goof-Off"
} from Guardian and it contains xylene.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} P. O. Box 11472 (letters)
} Guys Run Rd. (packages)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com {mailto:Walck-at-PPG.com}
}
} (412) 820-8651 (office)
} (412) 820-8515 (fax)
}
}
}
}
}




From daemon Tue Apr 16 15:34:53 2002



From: DrJohnRuss-at-aol.com
Date: Tue, 16 Apr 2002 16:28:14 EDT
Subject: Re: Plug in for Stereology Calculations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 4/16/02 2:25:55 PM, PhillipsT-at-missouri.edu writes:

} you could add it as a semi-transparent layer using Photoshop. To
} generate the sine wave, you could use any graphing program to plot
} out a sine wave and then copy it into Photoshop and erase the
} extraneous info like the X & Y axi.

What he wants is cycloids, not sine waves. You can generate these with the
Image Processing Tool Kit plug-ins for Photoshop (http://ReindeerGraphics.com)



From daemon Tue Apr 16 16:26:15 2002



From: Steve D'Angelo :      steve-at-equiprx.net
Date: Tue, 16 Apr 2002 14:12:58 -0700
Subject: Fwd: RE: goof-off not goop-off

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I have a small business repairing and refurbishing used laboratory instruments.
The instruments are frequently covered with tape, markings and labels
from years of use.
The product I like to use is a citrus based aerosol called 'Lift Off 2'.
This product is available at most hardware stores and will not affect
most plastics.
It does sometimes remove silk screened printing.
I have used Goof-Off, for very stubborn adhesives, for a few years
with mixed results.
The xylene can attack some plastics and paints.
I use this as a last resort only.
Very best regards,
Steve D'Angelo

Equipment Resurrection
1005 Terra Nova Boulevard, Suite 2
Pacifica, CA 94044.
650-738-0351

http://equiprx.net/




} From: Peter Tomic {PTomic-at-anadigics.com}
} To: "'Walck, Scott D.'" {walck-at-ppg.com} ,
} "Microscopy (E-mail)"
} {microscopy-at-sparc5.microscopy.com}
} Subject: RE: goof-off not goop-off
} Date: Tue, 16 Apr 2002 12:07:44 -0400
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Apr 16 16:29:02 2002



From: DULATT, KAREN R [AG/1000] :      karen.r.dulatt-at-Monsanto.com
Date: Tue, 16 Apr 2002 16:22:12 -0500
Subject: Postdoctoral Research Associate position here at the Monsanto Com

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Hello!

I have been made aware that some of you have had trouble responding to my
advertisement for Postdoctoral Research Associate position here at the
Monsanto Company.

Please if you are the least bit interested please respond to me.

Here is the advertisement that was sent out recently:

Monsanto values diversity and is an equal opportunity affirmative action
employer.


Title: Postdoctoral Research Associate

Group: Biotechnology
Function: Research & Development
Req Number: mons-00000241

Location(s): St. Louis MO
Responsibilities:

A two-year postdoctoral fellow position is available immediately for
developing, implementing, and applying advanced microscopy techniques to
elucidating the ultrastructure of plants, seeds, weeds and other biological
systems. Additionally, the selected individual is responsible for developing
new methods to improve the sample preparation protocols of biological
systems. The selected candidate will interact with multifunctional groups of
scientists working on biotechnology projects.
Required Skills:

The position requires a Ph.D. in plant biology or a related field with
experience in advanced electron and light microscopy techniques. A strong
background and extensive experience in TEM, high-resolution cryo-SEM, and
confocal laser scanning microscopy techniques are essential. Experience with
gene transformation in plants is a strong plus. The following key
competencies are desired: highly motivated and interested in developing new
imaging technologies; good interpersonal, verbal and written communication
skills; innovative and seeking opportunity to improve existing techniques
and processes.

If interest please visit our website at www.monsanto.com, or e-mail me back!




Karen Dulatt
Staffing Specialist of Technology
Ph: 314-694-5449
Fax: 314-694-6554




From daemon Tue Apr 16 17:26:47 2002



From: Curtis Olson :      COlson-at-scpglobal.com
Date: Tue, 16 Apr 2002 16:15:34 -0600
Subject: Kodak MDS 100 digital camera

Contents Retrieved from Microscopy Listserver Archives
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I am investigating an upgrade from a black & white video printer to the digital world for our Olympus BH3-MJLT Integrated Circuit Inspection microscope. The Sony B&W video printer specs indicate that the "Effective Pixels" are 700 dots x 472 lines (w/h). Ultimately, doesn't the printer quality determine the resolution? How does this quality relate to the resolution of the camera?

Does anyone have an opinion or experience with the seemingly simple but reasonably good resolution (1280 x 960) of the Kodak MDS 100? We would like to use this system to capture integrated circuits with both brightfield and darkfield light from x80 to approximately x2400.

The bottom line is, what are the important specs I should compare in order to be confident the new "system" will be better than what I already have?

Thank you all in advance.

Curtis



From daemon Tue Apr 16 19:51:35 2002



From: Ellis, Sarah :      s.ellis-at-pmci.unimelb.edu.au
Date: Wed, 17 Apr 2002 10:40:36 +1000
Subject: manual for a 2050 microtome

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Our manual for our Leica 2050 microtome (Histology) has gone missing. Can
anyone out there please help me out with a copy?

many thanks

Sarah Ellis

Head, Microscopy Research and Imaging Laboratory
Peter MacCallum Cancer Institute
Locked Bag #1, A'Beckett Street
East Melbourne 8006
Victoria
AUSTRALIA

ph 61-3-9656 1243
fax 61-3-9656 1411

email; s.ellis-at-pmci.unimelb.edu.au




From daemon Tue Apr 16 22:05:41 2002



From: J. A. Kiernan :      jkiernan-at-uwo.ca
Date: Tue, 16 Apr 2002 23:58:01 -0400
Subject: Re: Candle wax for Paraffin embedding?

Contents Retrieved from Microscopy Listserver Archives
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Fred, try these two sites for the terminology:
http://www.kodak.com/US/en/digital/ccd/sensorsMain.shtml and
http://www.ccd.com/ccdu.html . The second one is the former
http://www.apogee-ccd.com/.

Frame grabber- a very broad range of possibilities, in terms of cost, and
features/performance. You mentioned maximum exposure time of 4 minutes.
Regular video output can not handle exposures longer than 1/30 sec. A video
rate grabber for 768 x 494 resolution will be quite inexpensive, however, it
is a shame to waste long exposure capability of your camera. Does the camera
have any other output besides analog RGB (which you used to connect to the
monitor)? Does the camera have a control box with the video memory?

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Monson, Frederick C. {fmonson-at-wcupa.edu}
To: 'List-Microscopy' {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, April 16, 2002 12:45 PM


If it helps in any way:

_1._ When I came to UWO 30 years ago I followed local custom
(attributable to the late Ted Walker, who retired soon after
my arrival) and got my lab technician to use a mixture of
4 parts parowax (from supermarket) and one part of a commercial
wax and "polymer" mixture intended for histology. It didn't
seem to make any difference whether the special stuff was
Tissuemat or Paraplast. (Old Ted said parowax alone was a bit
too hard, and the posh stuff softened it a bit.) We used this
for about 12 years and then I changed to using just the special
histology wax at 100% - not because it was any better but
because it came as little pellets rather than slabs that took
a couple of hours to melt into the mixture.
For a small lab like mine the cost of using an expensive wax
is trivial, but big, busy outfits should seriously consider Ted
Walkers approach (which amounts to lowering the softening
temperature of a harder wax) and save a lot of money. Parowax
in the 1970s was a product of Shell (or ? of Esso). The
name now seems to be generic for slabs of paraffin about
10cm square and 1 cm thick, sold 3 to a box in supermarkets.

_2._ Russ Allison, a frequent Histonet contributor, has
investigated and compared a huge number of waxes and published
the results in peer-reviewed journals. He has summarized his
findings in Histonet messages, which should be findable at
www.histosearch.com (though that is a poor substitute for
reading the actual papers). Russ's most conspicuous conclusion
was that simple paraffin wax, without any additives, was as
good as any of the commercial or in-house mixtures.
If you're reading this, Russ, please denounce me if I have
misquoted your work.
John.
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan-at-uwo.ca
http://publish.uwo.ca/~jkiernan/
--
"Monson, Frederick C." wrote:
} The paraffin you get in the store is pure [you can use it to make candy!]!
} Embedding paraffin used to be made up. Here are some old recipes from Gray,
} P, "Microtomists Formulary and Guide", Krieger Publishing.
}
} Altman: 60degreeMP: paraffin 85, tristearin 10, beeswax 5.
} Beyer: paraffin 100, rubber 2, beeswax 0.5
} Gray 1941: paraffin(MP 58) 70, rubber 5, beeswax 5, spermaceti 5,
} nevillite "5" or clarite " 15 [see nailpolishes for this acrylic polymer]
} NOTE: this composition melts at about 50 but will cut 5um ribbons at room
} temp up to 85 degrees F.
} Pohlman: paraffin 10, bayberry wax 1
} Modern paraffin embedments are mixtures of paraffin and various
} other components including polyethylene glycols of different MW's (I think!)
}
} The above have to mixed thoroughly, and you must BELIEVE that there is NO
} other way!


From daemon Tue Apr 16 23:17:57 2002



From: Richard Thrift :      Richard_Thrift-at-SkyePharma.com
Date: Tue, 16 Apr 2002 21:13:40 -0700
Subject: Fwd: RE: goof-off not goop-off

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At home I use barbecue lighter fluid as a solvent for adhesive (& sharpie ink, etc). At work I use pentane or n-hexane. Works great, probably at least as as well if not better than anything else that's not particularly toxic. And these nonpolar solvents do not dissolve most paint or plastic.

Richard

} } } "Steve D'Angelo" {steve-at-equiprx.net} 4/16/02 2:12:58 PM } } }
Listers,
I have a small business repairing and refurbishing used laboratory instruments.
The instruments are frequently covered with tape, markings and labels
from years of use.
The product I like to use is a citrus based aerosol called 'Lift Off 2'.
This product is available at most hardware stores and will not affect
most plastics.
It does sometimes remove silk screened printing.
I have used Goof-Off, for very stubborn adhesives, for a few years
with mixed results.
The xylene can attack some plastics and paints.
I use this as a last resort only.
Very best regards,
Steve D'Angelo

Equipment Resurrection
1005 Terra Nova Boulevard, Suite 2
Pacifica, CA 94044.
650-738-0351

http://equiprx.net/




} From: Peter Tomic {PTomic-at-anadigics.com}
} To: "'Walck, Scott D.'" {walck-at-ppg.com} ,
} "Microscopy (E-mail)"
} {microscopy-at-sparc5.microscopy.com}
} Subject: RE: goof-off not goop-off
} Date: Tue, 16 Apr 2002 12:07:44 -0400
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Wed Apr 17 03:41:47 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 17 Apr 2002 09:33:55 +0100 (GMT Daylight Time)
Subject: Re: Running costs of FEG v. LaB6 TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi David,

We run both LaB6 and FEG TEMs here and, as with most
universities, our biggest cost worry is finding the money
for continual support of instruments. The costs of
ownership of LaB6 and FEG instruments are very different.
Assuming all other costs are the same (and if anything the
FEG needs better quality support than the Lab6) then it is
down to the tip costs.

A Lab6 tip will last about 1000 hours with a whenhelt clean
during that period. It depends on how much use your
instrument gets how long it takes to total your 1000
hours beam time.
A FEG tip will last much longer (25,000 to 30,000
hours?) but as it runs all the time this will be somewhere
in the region of 3 to 5 years.

A LaB6 tip change will take one or two days and can be
carried out by your own staff or a service engineer a tip
will cost less than £1000 (sterling) and two days engineer
time may be another £2000 (sterling).

We have not yet had a FEG tip change (only 26,000 hours)
but it will have to be carried out by service engineers and
can cost £20,000 to £30,000 (sterling).

So if your FEG tip lasts 5 years and costs say £25,000 to
replace it costs about £5000 per annum. If your LaB6 lasts
9 months and costs £3000 to replace it costs about £4000
per annum. No great difference - but if you use the
instrument less then the LaB6 cost reduces whereas the FEG
one does not. If you change LaB6 tips yourself the cost may
be about £1000 per annum. If you source the LaB6 tips
directly they may be as little as £500 each. This makes the
FEG considerably more expensive.

It is easier for us to find a few thousand pounds every
year rather than 20,000 to 30,000 every 3 to 5 years.
Although that is a budgeting problem it is often easier for
commercial companies to handle than for educational
institutions.

I can get HREM images from both my 2010 Lab6 and 3000F FEG
instruments within 5 to 10 minutes of inserting a specimen
and would expect the same of my CM20 LaB6 if it had the
high resolution pole piece. Both machines are very similar
to operate, the FEG is slightly more complicated to align
but you don't need to wait 2 minutes to run the tip up
after specimen insertion.

There are very obvious advantages to FEG instruments but
there are also disadvantages. They are higher brightness
sources but they do not emit as many electrons so low
magnification images are not as bright. They don't like
being switched off (power disturbances etc.) and take about
a day to condition and run up the tip after the gun vacuum
has been restablished (to a better level than LaB6).

I hope this helps,
Regards,
Ron

On Tue, 16 Apr 2002 17:06:17 +0100 David John
{djohn-at-tcd.ie} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} We are considering the purchase of a high resolution transmission electron
} microscope and I am interested in finding out the true costs of operating a
} FEGTEM versus a LaB6 TEM. Does a FEGTEM cost appreciably more to operate
} than a LaB6 and is the better resolution of the FEG it's only major
} advantage?
} I can obtain some information such as service contact costs from the
} manufacturers but I would like to hear of the experiences of actual users.
}
} Regards,
} David John
}
} Centre for Microscopy and Analysis,
} Trinity College,
} Dublin 2,
} Ireland.
}
} Tel. (353) - 1 - 6081559
} Fax. (353) - 1 - 6770438
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Apr 17 03:41:47 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Wed, 17 Apr 2002 10:33:47 +0200
Subject: 3CCD versus 1CCD color cameras and fluorescence microscopy

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Hi,

There are some additional aspects on the use of a single chip CCD versus
a triple chip CCD color camera. On the single CCD color camera the
spatial resolution in the direction where the 3 colors are interpolated,
e.g. R,G and B is one third (1/3) of the spatial resolution in the other
direction. In my opinion this makes them less suitable for high spatial
discrimination and quantitative microscopy.

There are single chip CCD cameras that use a filter changer to capture
the 3 color channels. This makes them slower and probably more sensitive
to pixel shifts. There are however some nice cameras on the market that
use this technology. You can probably even have one camera for both B/W
and color imaging.

A triple chip CCD color camera has the advantage that you can separately
regulate the 3 color chanels, which is an advantage for doing
fluorescence microscopy with several probes emitting with a different
quantum efficiency. I like these cameras for the freedom they allow you
to optimise the color image acquisition. But of course the more you can
manipulate on any system, the more mistakes you can make ;-)

For fluorescence microscopy of very faint signals, a computer controlled
(intensified) B/W camera combined with an automatics filter changer is
what gives you a lot of freedom. With this system the image acquisition
of each fluorescent probe can be controlled and optimized. This setup
also allows you to vary the integration time for each chanel separately.
Pixel shifts are of course a potential problem with this setup too. By
carefully chosing the probes and narrow band fluorescence filters,
crosstalk between the signals can be reduced and optimised for each
experiment.

Best regards,

Peter

P.S. this is my own personal opinion of about one eurocent. I have no
commercial relation with any of the camera manufacturers.
--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)
Cipalstraat 3
B-2440 Geel
Belgium
tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/
==============================================================
Chris Jeffree wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} One of the shortcomings of traditional single-chip colour ccds (I
} think all current compact digital cameras to date use single chips) is
} that the three colours at each location are measured by
} cc devices at three separate sample locations, which may have three
} separate intensities and colour values. The colour values must be
} estimated by interpolation within and between adjacent sensor groups,
} and there is scope for error in this estimation. The use of
} three-chip cameras is one way round this, using beam-splitters to
} project the components of the image onto three separate monochrome
} sensor arrays, the limitation here being that the image projection and
} alignment must be perfect. Recently Foveon has introduced a
} single-chip sensor, Foveon X3 in which for the first time three
} colours are measured at different depths at the same pixel location, a
} process which is the silicon equivalent of imaging with a colour film
} emulsion. Foveon X3 is said to eliminate the colour estimation errors
} associated with traditional ccds and to provide better spatial
} resolution per pixel as well. Is it too early for list members to have
} had hands-on experience of this very promising sensor?
}
} Chris
}
} Disclaimer: I have no financial interest in Foveon or their products
} other than a sharp price when (if) I buy them.


From daemon Wed Apr 17 04:21:24 2002



From: Philip Koeck :      Philip.Koeck-at-biosci.ki.se
Date: Wed, 17 Apr 2002 11:20:06 +0200
Subject: thickness and resolution

Contents Retrieved from Microscopy Listserver Archives
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Dear list members,

in the book 'Polymer Microscopy' by Sawyer and Grubb I read about a rule of
thumb relating
attainable resolution to the specimen thickness (about 1/15 of the thickness
for carbon specimens
imaged with 100 keV electrons).
The authors refer to the second edition of Reimer but I can't find this
statement in the fourth edition
anymore.

How seriously should this rule of thumb be taken for TEM phase contrast
imaging?
Does anybody know of a publication giving estimates for different electron
energies and specimen
thicknesses?

On pages 190ff in Reimer 4th ed. I found a discussion of the 'top-bottom
effect' in STEM with
some references to publications on similar effects in TEM and
energy-filtered TEM.
Would the resolution in a TEM phase contrast image depend on whether the
specimen
is above or beneath the support film?

Thankful for any comments and references,

Philip



From daemon Wed Apr 17 07:44:51 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 17 Apr 2002 08:40:36 -0400
Subject: Re: goof-off not goop-off

Contents Retrieved from Microscopy Listserver Archives
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John;

Thank you for that data. I personally have not used "terpene" for removing
carbon tape off of SEM stubs but I was curious if anyone had tried it. If I
recall, these terpene based materials were used to replace
chlorofluorocarbons that put that hole in the sky. We actually recycle our
solvents like 2-propanol and acetone in a process that is similar to
distilling, albeit it's done off-site. The recovery rate is pretty good.

Forgive my ignorance relative to the chemistry of terpene since I am but a
simple electrical engineer.

Regards,
Peter

-----Original Message-----
} From: John Twilley [mailto:jtwilley-at-sprynet.com]
Sent: Tuesday, April 16, 2002 4:15 PM
To: microscopy-at-sparc5.microscopy.com; Ptomic-at-anadigics.com


Peter,

The correct spelling of the class name is "terpene", in case anyone
intends to look it up, and they are certainly NOT non-flammable,
although they have higher flashpoints than some of the compounds that
they are replacing. Perhaps you have seen commercial formulations that
contain these solvents in combination with other agents. In this case,
there are some combinations that have very little flammability risk.

Some of the commercial preparations for stripping paint involve
citrus-derived terpenes and n-methylpyrrolidinone. There are many
others. Since the terpene constituents are not very water soluble, they
often include emulsification agents to assist in their dispersal in
rinse water.

Flammability remains something to be concerned about until the agent is
diluted. As a cautionary tale, many of the old citrus industry packing
houses met their end with catastrophic fires fueled by the "solvent" in
the fruit peels, a well aerated heap of grapefruit being a bit like
kerosene soaked green wood.

On the environmental side, one should note that while the defluxing
agent itself may be disposable without restriction, once used to de-flux
solder work done with lead-based solder, it will contain dissolved
lead-rosinate which most certainly cannot be disposed of in a "common
drain".

John Twilley

Peter Tomic wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
{mailto:ListServer-at-MSA.Microscopy.Com}
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} -----------------------------------------------------------------------.
}
}
} To All;
}
} Has anyone tried any terpine based solvents? I believe they are
derivatives
} of citrus fruits like lemons etc. and they seem to be used a great deal
} printed circuit board cleaning and de-fluxing. It is also an
} environmentally friendly material and may be disposed of in a common drain
} with no treatment and it's non-flammable.
}
} Peter Tomic
} Anadigics, Inc.
}
} -----Original Message-----
}
} } From: Walck, Scott D. [mailto:walck-at-ppg.com]
}
} Sent: Monday, April 15, 2002 9:51 AM
} To: Microscopy (E-mail)
} Subject: goof-off not goop-off
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
{mailto:ListServer-at-MSA.Microscopy.Com}
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Last week I mentioned that there was a good product on the market that
took
} off adhesive material better than acetone. The product name was
"Goof-Off"
} from Guardian and it contains xylene.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} P. O. Box 11472 (letters)
} Guys Run Rd. (packages)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com {mailto:Walck-at-PPG.com}
}
} (412) 820-8651 (office)
} (412) 820-8515 (fax)
}
}
}
}
}




From daemon Wed Apr 17 07:44:51 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Wed, 17 Apr 2002 10:08:44 -0230
Subject: RE: Running costs of FEG v. LaB6 TEM

Contents Retrieved from Microscopy Listserver Archives
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Jerzy writes ...

} ...
} our lab operates two TEMs, JEOL2010 LaB6 (6years old) and FEI
} CM300FEG (4 years old). ...
}
} The true cost to us is the down time. From my experience over the
} last three years, the JEOL runs 97% of the time, and CM ~80%
} (issues with cameras and attachments included). Our JEOL gets a
} new filament and set of apertures every six months with a PM. CM
} had it first tip exchanged after 4.5 years of service and it took
} a week to get it done. Our techs prefer to use the JEOL because
} of experience and ease of use.
} ...

Not exactly my experience with a JEOL 6400 with LaB6. That is, in spite
of the emitter only needing to be replaced every 800-1000 hours, the wehnelt
still needed to be cleaned every 100-200 hours for ease of use and long-term
stability. However, it would still mean a minimum of down-time (~1.5hrs)
.. extract the wehnelt on a late afternoon ... a quick bath in acidic
solution for dissolving the non-conducting deposits ... rinse with H2O and
dry etOH ... bake at 50C for 30min ... replace for the next day's projects.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From daemon Wed Apr 17 08:00:56 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 17 Apr 2002 08:59:19 -0400
Subject: Nikon DXM1200

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Folks;

Is there a standard that one may use when evaluating/comparing the image
reproduction ability of an electronic camera? For example, resolution,
color, aberration etc. comparison? Would this be something like an NTSC
resolution pattern used in broadcast video?

Peter Tomic

-----Original Message-----
} From: Damian Neuberger [mailto:neuberger1234-at-attbi.com]
Sent: Tuesday, April 16, 2002 1:53 PM
To: Microscopy-at-sparc5.microscopy.com


I've followed the thread on digital cameras and have decided to ask for
off-line opinions on the Nikon DXM1200 digital camera for attachment to
optical microscopes. I am not able to get as much information about the
camera's specifications as I would like. It is not cooled to the best of
my knowledge and it uses a rather unusual method of collecting an image,
pixel shift. A test yielded rather good images and compared favorably with
other cameras I've used, i.e better than others. The camera will be used
primarily for bright field color images but some polarized light, HMC and
DIC as well.

Damian Neuberger
Baxter Healthcare Corp
damian_neuberger-at-baxter.com or
neuberger1234-at-attbi.com




From daemon Wed Apr 17 09:25:31 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 17 Apr 2002 08:19:23 -0600
Subject: 3CCD versus 1CCD color cameras and fluorescence microscopy

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A slight correction:

Single chip CCD cameras mostly use so-called "Bayer-filters", which
essentially have a filter sequence R-G-B-G-R-G ..., i.e., they have twice as
many green sensitive pixels as red or blue sensitive pixles. This scheme is
related to the fact, that the human eye is most sensitive in the
green-yellow part of the spectrum. In other words, the resolution is NOT
1/3, it 1/2 at worst, but in reality better, unless you have a pure red or
pure blue image.

I'd also like to mention, that Fluorescence microscopes usually use filter
cubes that select both the illumination frequency as well as the
fluorescence color, so that an additional filter on a b/w camera is not
needed in most cases.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Peter Van Osta [mailto:pvosta-at-unionbio-eu.com]
Sent: Wednesday, April 17, 2002 2:34 AM
To: Chris Jeffree
Cc: microscopy-at-sparc5.microscopy.com


Hi,

There are some additional aspects on the use of a single chip CCD versus
a triple chip CCD color camera. On the single CCD color camera the
spatial resolution in the direction where the 3 colors are interpolated,
e.g. R,G and B is one third (1/3) of the spatial resolution in the other
direction. In my opinion this makes them less suitable for high spatial
discrimination and quantitative microscopy.

There are single chip CCD cameras that use a filter changer to capture
the 3 color channels. This makes them slower and probably more sensitive
to pixel shifts. There are however some nice cameras on the market that
use this technology. You can probably even have one camera for both B/W
and color imaging.

A triple chip CCD color camera has the advantage that you can separately
regulate the 3 color chanels, which is an advantage for doing
fluorescence microscopy with several probes emitting with a different
quantum efficiency. I like these cameras for the freedom they allow you
to optimise the color image acquisition. But of course the more you can
manipulate on any system, the more mistakes you can make ;-)

For fluorescence microscopy of very faint signals, a computer controlled
(intensified) B/W camera combined with an automatics filter changer is
what gives you a lot of freedom. With this system the image acquisition
of each fluorescent probe can be controlled and optimized. This setup
also allows you to vary the integration time for each chanel separately.
Pixel shifts are of course a potential problem with this setup too. By
carefully chosing the probes and narrow band fluorescence filters,
crosstalk between the signals can be reduced and optimised for each
experiment.

Best regards,

Peter

P.S. this is my own personal opinion of about one eurocent. I have no
commercial relation with any of the camera manufacturers.
--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)
Cipalstraat 3
B-2440 Geel
Belgium
tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/
==============================================================
Chris Jeffree wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} One of the shortcomings of traditional single-chip colour ccds (I
} think all current compact digital cameras to date use single chips) is
} that the three colours at each location are measured by
} cc devices at three separate sample locations, which may have three
} separate intensities and colour values. The colour values must be
} estimated by interpolation within and between adjacent sensor groups,
} and there is scope for error in this estimation. The use of
} three-chip cameras is one way round this, using beam-splitters to
} project the components of the image onto three separate monochrome
} sensor arrays, the limitation here being that the image projection and
} alignment must be perfect. Recently Foveon has introduced a
} single-chip sensor, Foveon X3 in which for the first time three
} colours are measured at different depths at the same pixel location, a
} process which is the silicon equivalent of imaging with a colour film
} emulsion. Foveon X3 is said to eliminate the colour estimation errors
} associated with traditional ccds and to provide better spatial
} resolution per pixel as well. Is it too early for list members to have
} had hands-on experience of this very promising sensor?
}
} Chris
}
} Disclaimer: I have no financial interest in Foveon or their products
} other than a sharp price when (if) I buy them.


From daemon Wed Apr 17 10:22:21 2002



From: Dr.Manfred Rohde :      mro-at-gbf.de
Date: Wed, 17 Apr 2002 17:16:31 +0100
Subject: pH for uranyl acetate

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Dear listers,

has anyone out there a protocoll for adjusting the pH value of a 2% aqueous
uranyl acetate solution/ or 2% solution in TE-buffer. I would like to have
a solution of a pH around 6.5-6.8.
I know of the procedure of Bruggen back from 1967: make 0.5% uranyl
acetate, dissolve 12 mM oxalic acid, adjust pH with diluted NH4OH- but this
solution is very, very unstable and in my hands the negative-staining is
not very proper. Does anyone have other suggestions.
Thank's in advance. Manfred



From daemon Wed Apr 17 10:41:19 2002



From: jtwilley-at-sprynet.com
Date: Wed, 17 Apr 2002 11:34:00 -0400
Subject: Re: Hexane

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Richard,

n-Hexane is a very useful non-polar solvent but
beware of its toxicity. My understanding is that
hexane exposure can be a cause of peripheral
neuropathy (nerve damage to the extremities
causing loss of sensations and spurious sensations
that can range from annoying to painful). The
odor of hexane and other "aliphatic" hydrocarbons
is less annoying than many "aromatics" such as
xylene or toluene so there is less of a warning
factor - meaning that people are more prone to
expose themselves. While hexane is less
implicated in liver and kidney damage than some
alternatives, I've heard that it is more of a
threat for peripheral neuropathy and that this is
seldom reversible.

As to lighter fluids and "odorless paint thinner",
I have always assumed that these are primarily
aliphatics and that the more volatile ones must
have an increased level of hexane relative to say,
kerosene or lamp oil.

DISCLAIMER: I am not a physician or occupational
health worker.

On Tue, 16 Apr 2002 21:13:40 -0700 Richard Thrift
{Richard_Thrift-at-SkyePharma.com} wrote:

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The
Microscopy Society of America


At home I use barbecue lighter fluid as a solvent
for adhesive (& sharpie ink, etc). At work I use
pentane or n-hexane. Works great, probably at
least as as well if not better than anything else
that's not particularly toxic. And these nonpolar
solvents do not dissolve most paint or plastic.

Richard


}


From daemon Wed Apr 17 12:08:01 2002



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Wed, 17 Apr 2002 10:03:45 -0700
Subject: Re: thickness and resolution

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Philip,

One limit to resolution in the HR-TEM is caused by limited interaction of the Ewald sphere with the specimen shape function at reciprocal lattice spikes at large scattering angles. This interaction goes to zero at a specimen thickness of Tmax = 2D*D/L, where D is resolution and L is electron wavelength.
At 300keV, this limit is 65 Angstrom thickness at 0.8 Angstrom resolution -- see figure 1 in “Sub-Ångstrom transmission electron microscopy at 300keV”, M.A. O’Keefe, E.C. Nelson, J.H. Turner and A. Thust in 59th Ann. Proc. MSA, Long Beach, California (2001) 898-899.
For other energies (200, 300, 400, 800, 1250keV), see figure 1 in: “Limits to spatial resolution in the HR-TEM”, Michael A. O’Keefe, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1165-1166.

Hope this helps,
Mike O'Keefe

Philip Koeck wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear list members,
}
} in the book 'Polymer Microscopy' by Sawyer and Grubb I read about a rule of
} thumb relating
} attainable resolution to the specimen thickness (about 1/15 of the thickness
} for carbon specimens
} imaged with 100 keV electrons).
} The authors refer to the second edition of Reimer but I can't find this
} statement in the fourth edition
} anymore.
}
} How seriously should this rule of thumb be taken for TEM phase contrast
} imaging?
} Does anybody know of a publication giving estimates for different electron
} energies and specimen
} thicknesses?
}
} On pages 190ff in Reimer 4th ed. I found a discussion of the 'top-bottom
} effect' in STEM with
} some references to publications on similar effects in TEM and
} energy-filtered TEM.
} Would the resolution in a TEM phase contrast image depend on whether the
} specimen
} is above or beneath the support film?
}
} Thankful for any comments and references,
}
} Philip



From daemon Wed Apr 17 13:03:27 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 17 Apr 2002 13:56:56 -0400
Subject: Great Learning Tools - DOE

Contents Retrieved from Microscopy Listserver Archives
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G'Day Listers,

In my WEB travels, I have come across some sites with both unusual and
useful material. In the site set out below, I found a plethora of material
that in part or whole should be of interest to many on out lists. The
Department of Energy (DOE) has a training program for folks who are going to
work in the general area of nuclear energy. In order to train these
personnel so that they have required knowledge of the systems with which
they will be working, DOE has instituted a Standards program for them. At
the site below, listers may find BOOKS in PDF form on Chemistry, Physics,
Math, Electronics, Instrumentation, Symbology(reading schematics) and more.
I thought that you all might be interested in bookmarking the site. I have
done so for my son who is doing his math and physics from the high school
texts. The DOE manuals represent a learning regime for new learners and
those who need review.

URL: http://tis.eh.doe.gov/techstds/standard/appframe.html

Regards,

Fred

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin/wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Wed Apr 17 15:23:08 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 17 Apr 2002 13:27:29 -0700
Subject: Re: pH for uranyl acetate

Contents Retrieved from Microscopy Listserver Archives
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Manfred

Aqueous uranyl-acetate solutions are not stable at pH higher than 5-5.5 I
believe. There is no way to make them stable. If you need higher pH you
could try uranyl-formiate. It's stable for a couple of hours on ice in the
dark and pH is higher than UA. Many years ago I was playing with oxalate
recipe (actually in this case you will have mixed uranyl-acetate/oxalate
salt solution) without any success. UF produced very nice staining and I
always use it for 'fine work'. You have to prepare UF fresh. Good
luck, Sergey.


At 05:16 PM 4/17/02 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Apr 17 15:43:42 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 17 Apr 2002 13:52:42 -0700
Subject: Re: Great Learning Tools - DOE

Contents Retrieved from Microscopy Listserver Archives
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Thanks Fred

It's really useful. Sergey

At 01:56 PM 4/17/02 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Apr 17 17:28:23 2002



From: Alan E. Davis :      adavis-at-saipan.com
Date: Thu, 18 Apr 2002 07:57:16 +1000
Subject: Plans for Scanning_Tunnelling Microscope

Contents Retrieved from Microscopy Listserver Archives
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I'm more a lurker and an amateur, but may I point out an interesting link to a "Scanning Probe Microscope Construction Kit"?

http://sxm4.uni-muenster.de/introduction-en.html

Cheers,

Alan Davis

--
Alan E. Davis, Science Instructor
Marianas High School
PMB 30, Box 10006,
Saipan, MP 96950
Northern Mariana Islands
adavis-at-saipan.com


"An inviscid theory of flow renders the screw useless, but the need
for one non-existent."
---Lord Raleigh(aka John William Strutt),or else
his son, Jr., who was also a scientist.


From daemon Wed Apr 17 18:42:51 2002



From: Paul Hazelton, PhD :      paul_hazelton-at-umanitoba.ca
Date: Wed, 17 Apr 2002 18:35:02 -0500
Subject: Re: pH for uranyl acetate

Contents Retrieved from Microscopy Listserver Archives
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manfred

you will not get a pH above about 4.0 with uranyl acetate. the only
uranyl salt which will give an elevated pH is uranyl oxalate. buy the UO
compound directly, do not try to make it yourself.

if you want an elevated pH try phosphotungstic acid or ammonium
molybdate instead. they are more stable than UO.

paul hazelton



From daemon Wed Apr 17 18:42:51 2002



From: Paul Hazelton, PhD :      paul_hazelton-at-umanitoba.ca
Date: Wed, 17 Apr 2002 18:36:49 -0500
Subject: Re: further to pH for uranyl acetate

Contents Retrieved from Microscopy Listserver Archives
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sorry, i missed your address site. in fact, being in germany, call RKI
and speak with hans gelderblom. he can give you a lot of information on
staining.

paul hazelton



From daemon Thu Apr 18 02:34:17 2002



From: Torsten.Fregin-at-zoologie.uni-hamburg.de
Date: Thu, 18 Apr 2002 09:22:26 +0200
Subject: RE: Video NTSC to PAL Conversion

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Hi,

yes, I am very sure, b/c I brought some tapes with me from my stay in the US, and my
Philips VCR has even a label saying "NTSC playback" on the front case. This function got
kind of important, because many Germans like to travel the US and buy tapes there (esp.
StarTrek fans)...
If you go to eg. www.philips.de, then to Produkte / Unterhaltungselektronik / VCR / Hifi-VCR
you will find out, that all of the recorders have this information:

NTSC-Wiedergabe
* Gibt Kassetten wieder, die mit NTSC aufgenommen wurden (z. B. Kassetten
aus den USA).

(My translation: NTSC-Playback: Playback for tapes in NTSC format, e.g. from the USA)


:-) Torsten

P.S. I found it interesting to learn that the US (in 1998/1999) were not as technologicaly
advanced as everyone thought in good old Europe: Cell phones as big as bricks (and truly
bad reception areas - even in South Africa in the middle of nowhere I got better
connections...), no "videotext" on TV (a text-based information system broadcasted by the
TV stations with news, weather reports, TV program, stock exchange rates, and so on) and
else. But I will stop now b/c I don't want to use this mailing list for a European propaganda
campaign...

}
}
} Thorsten,
}
} are you sure about NTSC and PAL VCR's? Or are you talking about
} PAL/SECAM VCRs? PAL/SECAM would make more sense, because the two
} signals use the same bandwidth with a different scheme to transfer the
} color information. NTSC uses a different bandwidth. Also, PAL and
} SECAM are both used in Europe (Secam: France, PAL: rest of Europe),
} while NTSC is used in the US.
}
} mike
}
} } } } } } } } } } } WE HAVE MOVED { { { { { { { { {
} please make a note of the new address below
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: "Torsten.Fregin-at-zoologie.uni-hamburg.de"-at-sparc5.microscopy.com
} [mailto:"Torsten.Fregin-at-zoologie.uni-hamburg.de"-at-sparc5.microscopy.com
} ] Sent: Monday, March 25, 2002 4:09 AM To:
} Microscopy-at-sparc5.microscopy.com; Peter Tomic Subject: Re: Video NTSC
} to PAL Conversion
}
}
} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} Hi Peter (and everyone else),
}
} most (new) VCRs in Germany/Europe (as the companies sell them all over
} Europe, in most cases with thick handbooks in all languages) can
} (re)play both video standards, so if your associate has a new VCR (or
} buys one for 150 EUR), you should not have any problems. Just send a
} tape. Vice versa (PAL to NTSC) it is a problem... Some universities
} have media centers, you can get copies at low cost (~ $ 15 per tape;
} but that is for education purposes).
}
} :-) Torsten
}
}
} }
} }
} } Folks;
} }
} } Is anyone aware of any PC based software/hardware package that could
} } be used to easily convert from U.S. NTSC video standard to European
} } PAL standards? I have some lab. procedures and tests I'd like to
} } send to an associate in Europe and I'd like to not have to convert
} } it to digital .mpg or .avi format since the file size would be
} } inordinately large for the period of time the video requires. I
} } know there are a few firms that will do the conversion if I send the
} } VHS video tape out but I'd love to have that capability at a
} } desktop.
} }
} } Regards,
} } Peter Tomic
} } Group Leader
} } Failure Analysis & Analytical Services
} } Anadigics, Inc.
} } 141 Mt. Bethel Road
} } Warren, New Jersey
} } U.S.A.
} }
}
}
}
}
} Torsten Fregin
}
} Universität Hamburg - Zoologisches Institut
} Abt. Neurophysiologie
} AG Wiese - Raum 413
} Martin-Luther-King-Platz 3
} 20146 Hamburg, Germany
} Telefon *49-(0)40-42838-3931
} Fax *49-(0)40-42838-3937
} eMail Torsten.Fregin-at-zoologie.uni-hamburg.de
} oder TorstenFregin-at-gmx.de
}
}
}
}




Torsten Fregin

Universität Hamburg - Zoologisches Institut
Abt. Neurophysiologie
AG Wiese - Raum 413
Martin-Luther-King-Platz 3
20146 Hamburg, Germany
Telefon *49-(0)40-42838-3931
Fax *49-(0)40-42838-3937
eMail Torsten.Fregin-at-zoologie.uni-hamburg.de
oder TorstenFregin-at-gmx.de





From daemon Thu Apr 18 07:57:20 2002



From: David Waugh :      dwaugh-at-kent.edu
Date: Thu, 18 Apr 2002 08:49:48 -0400
Subject: Failed EDAX

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Our department as acquired a Amray 1645 with an EDAX unit (9900). The
SEM works well, but the Edax, which worked for the previous owner has
yet to function. It gets around 900 cps and generates two peaks with
low eV (less than one), the problem is that it gets the same cps with
the SEM off. This leads me to believe the detector is bad, it has
been cooled on and off, since we have had it, but it may has sat for
a year or two with no LN before that. I have used the manual
calibration to adjust the "gain" and "zero" with no change in the
spectrum generated. We don't have the money to have a real technician
look at it, does anybody have any suggestions or simple, or not so
simple, things I can try to at least narrow down the problem?
Thanks
David


From daemon Thu Apr 18 08:08:42 2002



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Thu, 18 Apr 2002 15:53:51 +0200
Subject: European NanoBusiness Association

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The comment below about phosphotungstic acid reminds me to ask a question I
have had. Is, or for how long is phosphotungstic acid, stored as powder in
the original bottle, good for. Through this list I learned that bottles of
powdered uranyl acetate are not good forever. Is the same true for
phosphotungstic acid?

Thank you,
Maureen Petersen
University of Florida
Dept of Plant Pathology


-----Original Message-----
} From: Paul Hazelton, PhD [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Wednesday, April 17, 2002 7:35 PM
To: Dr.Manfred Rohde
Cc: Microscopy-at-sparc5.microscopy.com


Hi,

Some of you may be interested in the launch of a new independent non-profit organization, the European NanoBusiness Association (www.nanoeurope.com).

The Association aims to provide a neutral platform in which the business, academic and financial communities can come together to:

· Discuss and promote the development of a dynamic nanotechnology industry
· Build a common forum through which to rapidly share and disseminate well-researched and realistic information for its members and for public education
· Promote the development of promising technology arenas
· Connect its members with the local and global nanotechnology community
· Monitor and benchmark Europe's competitive position in relation to the building and commercialization of nanotechnology

Naturally, microscopy is an important part of this.

Best

Tim


*****************************************************************
CMP Cientifica
Empowering business to make rational decisions about nanotechnology

Tim E. Harper - CEO

http://www.cmp-cientifica.com/





From daemon Thu Apr 18 10:09:58 2002



From: Paul Hazelton, PhD :      paul_hazelton-at-umanitoba.ca
Date: Thu, 18 Apr 2002 10:00:17 -0500
Subject: Re: pH for uranyl acetate

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maureen-

totally unscientific. i compared a bottle of PTA powder which had been
in the fridge for 20 years with a newly obtained bottle. different
suppliers, the older bottle had been stored at 4C, sealed with
parafilm. therefore, optimal storage conditions. there was no apparent
difference to me. but then it was not blinded.

i also have compared solutions of PTA which have been made up, stored in
brown glass at 4C, small head of air (intentionally minimized), sealed
with parafilm. again, ideal storage and unblinded. i saw no apparent
difference between fresh, 2 month old, 6 month old and 1 year old
preparations in terms of precipitate formed, haziness, pH, or quality of
stain. again, unblinded observations

having said this, our protocol is to make new stock solutions every 6-8
weeks. also, we filter all stain solutions at time of preparation
through 0.2 micron bottle top filters, and again just prior to use with
syringe filters, pore size 0.2 micron.

we usually use the stock powder PTA bottle as obtained from the supplier
for 10-15 years.

paul hazelton



From daemon Thu Apr 18 11:36:30 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 18 Apr 2002 12:28:47 -0400
Subject: FW: Image Standard for Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Again Peter,
finding even more good stuff on performance analysis of imaging
systems.

Even free stuff: http://www.mitre.org/technology/mtf/

This might really help to answer questions.

Fred

} ----------
} From: Monson, Frederick C.
} Sent: Thursday, April 18, 2002 11:51 AM
} To: 'Peter Tomic'
} Subject: RE: Image Standard for Microscopy
}
} Morning Peter,
}
} On this matter, Castleman, in Shotten's Electronic Light Microscopy,
} makes use of what he calls a "sine wave bar target" (a test pattern) to
} measure the resolution of an imaging system. I think a more specific
} explanation may be found at:
} http://www.pvinc.com/tutorial/tutorial-quality-intro.htm.
} Sources for targets are given on the 10th page of this PDF:
} http://www.mitre.org/support/papers/tech_papers_01/nill_conversion/nill_co
} nversion.pdf. Note, this PDF is a discussion of Modulation Transfer
} Function(MTF) and Contrast Transfer Function(CTF). Sine Pattern Producer
} is: http://www.sinepatterns.com/.
}
} Hope this helps,
}
} Fred Monson
}
} P.S. Tubes in box!!!
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} West Chester University
} South Church Street
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin/wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
}
}
} ----------
} From: Peter Tomic
} Sent: Wednesday, April 17, 2002 8:59 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: Image Standard for Microscopy
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Folks;
}
} Is there a standard that one may use when evaluating/comparing the image
} reproduction ability of an electronic camera? For example, resolution,
} color, aberration etc. comparison? Would this be something like an NTSC
} resolution pattern used in broadcast video?
}
} Peter Tomic
}
} -----Original Message-----
} } From: Damian Neuberger [mailto:neuberger1234-at-attbi.com]
} Sent: Tuesday, April 16, 2002 1:53 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Nikon DXM1200
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I've followed the thread on digital cameras and have decided to ask for
} off-line opinions on the Nikon DXM1200 digital camera for attachment to
} optical microscopes. I am not able to get as much information about the
} camera's specifications as I would like. It is not cooled to the best of
} my knowledge and it uses a rather unusual method of collecting an image,
} pixel shift. A test yielded rather good images and compared favorably
} with
} other cameras I've used, i.e better than others. The camera will be used
} primarily for bright field color images but some polarized light, HMC and
} DIC as well.
}
} Damian Neuberger
} Baxter Healthcare Corp
} damian_neuberger-at-baxter.com or
} neuberger1234-at-attbi.com
}
}
}
}
}


From daemon Thu Apr 18 12:32:52 2002



From: Louise_Harner-at-albint.com
Date: Thu, 18 Apr 2002 13:53:15 -0400
Subject: Re: European NanoBusiness Association

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sender, E-mmunity has detected virus(es) in your e-mail attachment(s).

Method: Mail



I believe this should have been:
www.nanoeurope.org

- Louise
Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com




"Tim E. Harper"
{tim-at-cmp-cientifi To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
ca.com} cc:
Subject: European NanoBusiness Association
2002/04/18 09:53
AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

Some of you may be interested in the launch of a new independent non-profit
organization, the European NanoBusiness Association (www.nanoeurope.com).

The Association aims to provide a neutral platform in which the business,
academic and financial communities can come together to:

· Discuss and promote the development of a dynamic
nanotechnology industry
· Build a common forum through which to rapidly share and
disseminate well-researched and realistic information for its members and
for public education
· Promote the development of promising technology arenas
· Connect its members with the local and global nanotechnology
community
· Monitor and benchmark Europe's competitive position in
relation to the building and commercialization of nanotechnology

Naturally, microscopy is an important part of this.

Best

Tim


*****************************************************************
CMP Cientifica
Empowering business to make rational decisions about nanotechnology

Tim E. Harper - CEO

http://www.cmp-cientifica.com/










From daemon Thu Apr 18 12:59:21 2002



From: Louise_Harner-at-albint.com
Date: Thu, 18 Apr 2002 13:53:15 -0400
Subject: Re: European NanoBusiness Association

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I believe this should have been:
www.nanoeurope.org

- Louise
Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com




"Tim E. Harper"
{tim-at-cmp-cientifi To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
ca.com} cc:
Subject: European NanoBusiness Association
2002/04/18 09:53
AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

Some of you may be interested in the launch of a new independent non-profit
organization, the European NanoBusiness Association (www.nanoeurope.com).

The Association aims to provide a neutral platform in which the business,
academic and financial communities can come together to:

· Discuss and promote the development of a dynamic
nanotechnology industry
· Build a common forum through which to rapidly share and
disseminate well-researched and realistic information for its members and
for public education
· Promote the development of promising technology arenas
· Connect its members with the local and global nanotechnology
community
· Monitor and benchmark Europe's competitive position in
relation to the building and commercialization of nanotechnology

Naturally, microscopy is an important part of this.

Best

Tim


*****************************************************************
CMP Cientifica
Empowering business to make rational decisions about nanotechnology

Tim E. Harper - CEO

http://www.cmp-cientifica.com/










From daemon Thu Apr 18 13:13:33 2002



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Thu, 18 Apr 2002 20:07:14 +0200
Subject: Re: European NanoBusiness Association

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oops! That should of course have been www.nanoeurope.org.

Tim

++++++++++++++++++++++++++++++++++
CMP Cientifica http://www.cientifica.com
Empowering Business to Make Rational Decisions
About Nanotechnology

Tim Harper CEO
Ph +34 91 640 71 85

-----Original Message-----
} From: Louise_Harner-at-albint.com [mailto:Louise_Harner-at-albint.com]
Sent: 18 April 2002 19:53
To: tim-at-cmp-cientifica.com; Microscopy-at-MSA.Microscopy.Com;
Microscopy-at-sparc5.microscopy.com


Hi,

Some of you may be interested in the launch of a new independent non-profit
organization, the European NanoBusiness Association (www.nanoeurope.com).

The Association aims to provide a neutral platform in which the business,
academic and financial communities can come together to:

· Discuss and promote the development of a dynamic
nanotechnology industry
· Build a common forum through which to rapidly share and
disseminate well-researched and realistic information for its members and
for public education
· Promote the development of promising technology arenas
· Connect its members with the local and global nanotechnology
community
· Monitor and benchmark Europe's competitive position in
relation to the building and commercialization of nanotechnology

Naturally, microscopy is an important part of this.

Best

Tim


*****************************************************************
CMP Cientifica
Empowering business to make rational decisions about nanotechnology

Tim E. Harper - CEO

http://www.cmp-cientifica.com/














From daemon Thu Apr 18 14:07:58 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 18 Apr 2002 14:01:41 -0500
Subject: Re: Failed EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My Oxford ISIS system has the same symptoms when the window ices up after a
rare chamber opening. The ISIS has a detector conditioning mode that lets
you de-ice the detector safely but I'm not that familiar with the EDAX
unit. You could let the detector warm up to room, then make sure the
chamber is under a good vacuum, then re-cool the unit. Another caveat:
make sure the detector housing is not touching anything (like the pole
piece) the the chamber. Once I had the same symptoms as ice on the
detector, but 2-3 conditioning cycles didn't change a thing. I finally
called field service and the supervisor told me to back the detector out a
couple of turns to make sure it wasn't touching anything. Sure enough, that
fixed it. When I finally had a chance to get inside the chamber and take a
look, it was obvious that when the detector was in to the stop, it was
barely touching the pole piece, causing a ground loop or something. So I
backed it out 2 turns and reset the stop. No more problem.
Good luck.

David Waugh wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Our department as acquired a Amray 1645 with an EDAX unit (9900). The
} SEM works well, but the Edax, which worked for the previous owner has
} yet to function. It gets around 900 cps and generates two peaks with
} low eV (less than one), the problem is that it gets the same cps with
} the SEM off. This leads me to believe the detector is bad, it has
} been cooled on and off, since we have had it, but it may has sat for
} a year or two with no LN before that. I have used the manual
} calibration to adjust the "gain" and "zero" with no change in the
} spectrum generated. We don't have the money to have a real technician
} look at it, does anybody have any suggestions or simple, or not so
} simple, things I can try to at least narrow down the problem?
} Thanks
} David

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Apr 18 15:34:27 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 18 Apr 2002 16:29:31 -0400
Subject: Failed EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David;

There are several possible problems that come immediately to mind and should
be checked.

Is there an IR. chamberscope illumination source left on while acquiring a
spectra? This will drive the detector crazy and give you a big peak at the
extreme low energy portion of the spectra.
Cabling issues, of course.
Detector diode non-functional.

Try changing the accelerating voltage higher to see if the count rate
increases. If not, you may just be measuring thermal noise in the detector
and not x-ray signal at all. Increasing the beam current will also raise
the count rate and is a good check.

EDAX 9900? Is this an oldie? If I recall, it has floppy drives the size of
a pizza, 10".

Hope this is of some help.

Regards,
Peter Tomic
Anadigics, Inc.


-----Original Message-----
} From: David Waugh [mailto:dwaugh-at-kent.edu]
Sent: Thursday, April 18, 2002 8:50 AM
To: Microscopy-at-sparc5.microscopy.com


Our department as acquired a Amray 1645 with an EDAX unit (9900). The
SEM works well, but the Edax, which worked for the previous owner has
yet to function. It gets around 900 cps and generates two peaks with
low eV (less than one), the problem is that it gets the same cps with
the SEM off. This leads me to believe the detector is bad, it has
been cooled on and off, since we have had it, but it may has sat for
a year or two with no LN before that. I have used the manual
calibration to adjust the "gain" and "zero" with no change in the
spectrum generated. We don't have the money to have a real technician
look at it, does anybody have any suggestions or simple, or not so
simple, things I can try to at least narrow down the problem?
Thanks
David


From daemon Thu Apr 18 17:06:43 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 18 Apr 2002 16:59:10 -0500
Subject: Re: Failed EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The window on our Kevex Quantum developed a pinhole some years back. I
remember the count-rate and dead-time being excessive even when the beam
was turned off. I don't know if it was as low as what you are reporting.
Air had gotten past the window and was causing noise. As the chamber was
left under vacuum (for hours), the vacuum in the detector eventually
improved so that the background count-rate dropped to normal levels and we
could operate. However, whenever we vented the sample chamber (as opposed
to using the airlock), we again saw the jump in the resting count rate.

We took the detector off and could just see the pinhole using a loupe. We
sent the detector in for repair, and it has been fine since.

You say the detector has also been cooled on and off. I trust the high
voltage to the detector was turned off whenever the detector was allowed to
warm up. I understand that applying voltage to a warm Si(Li) detector can
ruin it in short order.

Warren

At 08:49 AM 4/18/02 -0400, you wrote:

} Our department as acquired a Amray 1645 with an EDAX unit (9900). The SEM
} works well, but the Edax, which worked for the previous owner has yet to
} function. It gets around 900 cps and generates two peaks with low eV (less
} than one), the problem is that it gets the same cps with the SEM off. This
} leads me to believe the detector is bad, it has been cooled on and off,
} since we have had it, but it may has sat for a year or two with no LN
} before that. I have used the manual calibration to adjust the "gain" and
} "zero" with no change in the spectrum generated. We don't have the money
} to have a real technician look at it, does anybody have any suggestions or
} simple, or not so simple, things I can try to at least narrow down the problem?
} Thanks
} David

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Fri Apr 19 08:04:37 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw (by way
Date: Fri, 19 Apr 2002 07:46:53 -0500
Subject: Microwave Ovens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

I have no experience with microwave fixation. Our laboratory ma be in the
market for one. What are users experience with microwaves. What are the
limitations and quality of fixation versus conventional techniques? What
about normal household microwave ovens. Please share your experience good
and bad, offline please.

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana
{ {...OLE_Obj...} }

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}


From daemon Fri Apr 19 09:08:10 2002



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Fri, 19 Apr 2002 10:00:32 -0400
Subject: Uranyl Acetate Donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I was asked by an EHS person to see if I could find a way to recycle or donate a few one pound bottles of uranyl acetate to someone to avoid lab-packing it.
These unused bottles of material are about 20 years old and they are in the original one pound bottles.

It was used to do gravametric sodium determinations but I know it is also used in TEM. The purchaser has retired and we want to get rid of this 100 year supply of material. It is depleted uranium and is very slightly radioactive.

Is this stuff still good after all these years?
I thought is was until I read an email yesterday.
Is there a simple test I can do to determine if it's still good?

Any practical test suggestions off list would be helpful.

I do not have this material released to me for disposal but I have it near my lab. If you are interested, please email me directly by email. For clarity, donation means free for pre-paid shipping charges or personal pick up in Monroeville, PA.

We reserve the right to discriminate and/or cancel this offer. US citizens and institutions only, for example. You assume all risks in use and disposal of this material after receiving it.

Paul Beauregard
Senior Research Associate
PPG Industries
440 College Park Drive
Monroeville, PA 15146
724-325-5131




From daemon Fri Apr 19 09:29:14 2002



From: Stephen.R.Poe-at-aphis.usda.gov
Date: Fri, 19 Apr 2002 10:23:00 -0400
Subject: More alternatives to Goof-Off

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am another one of those folks that works a lot with older microscopes -
often loaded up with tape and stickers. Seems that the worst is petrified
masking tape. For a long time my favorite solvent was Finish Line citrus
bicycle chain cleaner. Got most stickum off right away, and for the really
severe cases would soften even the worst when you soaked it for about 30
minutes (usually done with a soaked paper towel folded up wraped in saran
wrap). Been hard to get the chain cleaner lately so I have been using a
cleaning product called De-Solve-It - another citrus based product that
seems to work nearly as well. I have tried a lot of other solvents, but
these two really do the job and do not seem to affect paint and finishes.
BTW, once you get all the tape and stickers off I find that Miguires #6 car
cleaner and polish does a fine job - very slight abrasive, but still gentle
enough to be used on guitars (my son was a guitar repair person and
suggested this). Amazing how good something like a funky old Leitz Labolux
can look after a couple hours of cleaning.

Stephen



From daemon Fri Apr 19 11:02:08 2002



From: AMCGroup2-at-aol.com
Date: Fri, 19 Apr 2002 11:51:55 EDT
Subject: Job Opening: TEM Analyst

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


AMC Group is a contract R&D consultancy based in Albuquerque, NM. We are
currently seeking a graduating or recent Ph.D. graduate with hands-on
academic background in TEM of semiconductor materials and devices.
Experience should include specimen preparation by broad-ion beam (BIB)
milling, focused ion beam (FIB) milling, and wedge-polishing. Assistance in
obtaining work permit will be provided to US non-residents or citizens. To
apply, please send a letter of application and current resume off-line to
amcgroup2-at-aol.com.

James Glossinger
Sr. Scientist
AMC Group


From daemon Fri Apr 19 13:20:32 2002



From: Christopher Gilpin :      christopher.gilpin-at-utsouthwestern.edu
Date: Fri, 19 Apr 2002 13:11:34 -0500
Subject: EM Technician position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
This is an advance notice that we will soon be advertising for an EM
technician post here at UT southwestern Medical Center in Dallas.
I would be happy to hear from anyone with experience in TEM with a
willingness to learn and develop.
Facilities here are good and getting better!

Regards

Chris



Christopher J. Gilpin
Assistant Professor
Director Molecular and Cellular Imaging Facility
K1.246
Department of Cell Biology
University of Texas Southwestern Medical Center
5323 Harry Hines Boulevard
Dallas, Texas 75390-9039
+1 214 648 2827 Phone
+1 214 648 6408 Fax
christopher.gilpin-at-utsouthwestern.edu



From daemon Fri Apr 19 17:12:48 2002



From: =?iso-8859-1?q?Ike=20Oguocha?= :      oguocha-at-yahoo.com
Date: Fri, 19 Apr 2002 23:03:56 +0100 (BST)
Subject: Help: Lattice Parameters of Nickel Phosphides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friends:

I'm looking for the the lattice parameters of the following
nickel phosphides. I could not find the info from the old
powder diffraction data handbook (published around 1968)
in our lab. You help will be highly appreciated.

-----------------------
Phase PDF No.
Ni3P 34-0501
Ni2P 03-0953
Ni5P4 18-0883
NiP2 21-0590 and/or 13-0213
Ni12P5 22-1190
Ni5P2 17-0225
Ni7P3 03-1101
------------------------

Many thanks in advance.

Ike Oguocha


__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com


From daemon Fri Apr 19 19:17:00 2002



From: Offers :      Offers-at-allbestcheapstuff.com
Date: Fri, 10 Sep 1999 08:33:29 -0400
Subject: Your approval is needed

Contents Retrieved from Microscopy Listserver Archives
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entered into our CASH SWEEPSTAKES for $25,000
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From daemon Mon Apr 22 04:56:15 2002



From: e-mmunity-at-electric.net
Date:
Subject: A IE 6.0 patch

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sender, E-mmunity has detected virus(es) in your e-mail attachment(s).

Method: Mail


From daemon Mon Apr 22 06:49:55 2002



From: Kelloes, Cathy L :      KELLOECL-at-bp.com
Date: Mon, 22 Apr 2002 06:40:14 -0500
Subject: Carbon particle identification in Polypropylene Films

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Hello All,

I would like some input on identifying carbon particle distribution in
polypropylene films.

Since the particles are approximately 19nm, I was thinking of using Spurr
resin and doing TEM; however, I would like to hear from some of you that
have had experience with carbon identification. Is this my best bet or
would there be a better, less time consuming method. Thanks in advance.
Cathy

Cathy Kelloes
Microscopy Technician
BP, Amoco FFBU
260 The Bluffs
Austell, GA. 30168
Phone: 770-941-1711, ext. 3255
Fax: 770-944-4745
E-mail: kelloecl-at-bp.com

This message is made of 100% recycled electrons.



From daemon Mon Apr 22 07:46:10 2002



From: msteglic-at-mail.mdanderson.org
Date: Mon, 22 Apr 2002 07:41:30 -0500
Subject: B&W print processor

Contents Retrieved from Microscopy Listserver Archives
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I will soon be replacing my current B&W print processor in my darkroom. I
know that there used to be several on the market but I have only been able to
} locate one,the MohrPro. Any feed back on this processor or any other one
currently
available along with estimated costs would be greatly appreciated.

Mannie Steglich




From daemon Mon Apr 22 09:46:05 2002



From: Brian J Laughlin :      brjlau18-at-US.ibm.com
Date: Mon, 22 Apr 2002 10:36:40 -0400
Subject: TEM: earthquake damage to a microscope

Contents Retrieved from Microscopy Listserver Archives
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Over the weekend we east coasters were in for quite a surprise in that we
had a 5.1 magnitude earthquake. The epicenter was about 25 miles from our
laboratory. I am just curious about what sort of damage small earthquakes
could cause to a sensitive instrument like a TEM. Are most air tables and
anti-vibration systems sufficient to absorb most of the shock? Are any of
my alignments in jeopardy? Is there anything that I should specifically
check besides just using the instrument to see if it working properly? We
have a JEOL 2010F and a Phillips CM-30. We also have a FIB tool, and my
concern that some liquid Ga could have been knocked off into the column
seems to be unfounded.

I am sure those of you on the west coast must deal with this on a regular
basis and can provide some insight. The major difference being that your
buildings are probably built to a more "earthquake-proof" code than any of
ours are.

Sincerely,

Brian Laughlin

FIB/TEM Engineer
IBM, Burlington, VT
Microelectronics Division
Surface and Materials Science Laboratory (Dept. GP8)


Lab: Bldg. 967-1 N18, (802) 769-1596
Fax: (802) 769-1220
Mail: IBM Burlington, 1000 River St., Essex Junction, VT 05452 Mailstop
967L





From daemon Mon Apr 22 11:00:33 2002



From: McCarthy, Deborah :      DMccar-at-lsuhsc.edu
Date: Mon, 22 Apr 2002 10:47:06 -0500
Subject: Rick Harris's question about epon embedded thicks

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Hi Rick,
Several years ago I had the job of taking vibratome sections, reacting them
for HRP product and then basically infiltrating them with epon but embedding
them on a slide that had been treated with Sigmacote (Sigma Chemical Co.)
prior to use. I may have even treated the coverslips also. The reason we
chose to do this is because we had to do camera lucida drawing of filled
neurons prior to serial EM sectioning and reconstruction. Hope this helps.

Deborah McCarthy
Dept. of Pathology
LSUHSC
Shreveport, LA 71106
dmccar-at-lsuhsc.edu


From daemon Mon Apr 22 13:04:45 2002



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Mon, 22 Apr 2002 13:56:01 -0400
Subject: Re: B&W print processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mannie,
We have an Ilford processor that we're very happy with . Its cost was about
$8000 and it's larger than the Mohr (30"x35"x19"), if space is an issue for
you . The company is Ilford Imaging USA, Inc and they're located in Paramus
, NJ , phone number 800-631-2522. Good luck.
Mary Gail Engle


At 07:41 AM 4/22/02 -0500,
msteglic-at-mail.mdanderson.org"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-9700


From daemon Mon Apr 22 13:30:39 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Mon, 22 Apr 2002 14:24:44 -0400
Subject: Wrinkled Thicks II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

Thanks to all who responded to my query about wrinkles. We have tried a different mounting media but a few of the sections are still wrinkling.

These sections are flat ( I checked) before I put on the Permount, and then they get wrinkly (like I do when I stay in the tub too long ;-) ).

My question is this: Does the temperature of the slide and mounting media matter? My slides are still hot, the Permount or CytoSeal is room temp. Can that cause the wrinkles? Should both be warm or room temp?

Massive frustration abounds....

I think I'll get a Shar Pei and admire his wrinkles,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Mon Apr 22 15:06:08 2002



From: Jay Campbell :      jmcampbe-at-facstaff.wisc.edu
Date: Mon, 22 Apr 2002 14:57:58 -0500
Subject: Freeze Sub & Grid staining

Contents Retrieved from Microscopy Listserver Archives
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--
Hello Listers,
I have 2 questions which are only tangentially related in that they
both deal with TEM techniques.

1) What is your favorite medium for freeze-substitution? We would
like to do a comparative study of several different fixative mixtures
to see what will work best for our samples. I will be happy to
receive entire protocols, or just solvents and fixative %ages.

2) What is your favorite method for staining large numbers of grids
(say 70-150) uniformly? I have tried a few methods and each seems to
have a drawback, ie. poor rinse vs. punctured formvar. What methods
work best for you?

Contact me on or offline and if there is sufficient interest, I will
post a compilation of the feedback to the list.
Thanks in advance,
Jay


Jay Campbell
Associate Research Specialist
University of Wisconsin
Dept.of Anatomy and Molecular Bio.
jmcampbe-at-facstaff.wisc.edu
608 263 8481 voice
608 265 3083 fax


From daemon Mon Apr 22 15:07:26 2002



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Mon, 22 Apr 2002 12:53:53 -0700
Subject: HPF and Cryo-EM Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a reminder that the deadline for applications for the
International Cryo-EM course in June is fast approaching - April 30th.

This is very much a hands-on course where participants should bring
their own cells/specimens/liposomes where ever possible, and finish
with some publishable results. This is the ideal but no big deal if
students just want to learn the techniques. We will provide
specimens. No previous knowledge is necessary.

Participants will have the opportunity to use high pressure freezers,
slam freezers, plunge freezers, TEM and SEM with cryo stages,
cryo-ultramicrotomes, cryo substitution systems, immunolabelling
techniques. Expertise will be on hand for all stages of the course
and participants will not be expected to cut their own blocks unless
they want to. Assistance will be given to ensure participants are
able to put together a presentation of their results.

This course is sponsored by Baltec (Technotrade), Emitech, Gatan,
Hitachi, Leica, Pelco International and Quantifoil Microtools. It is
organised by Kent MacDonald (Berkeley), Stan Erlandsen (Minnesota)
and Elaine Humphrey (UBC).

Please let your co-workers know about this course.

The application form can be found at http://www.emlab.ubc.ca

We have been asked if persons can attend this course for just two or
three days. Either they only want to use the high pressure freezer or
they are only interested in cryo-SEM. We have agreed in principle on
the basis of $275/day (includes lunch). The schedule will be
available shortly but overall it will be high pressure freezing in
the first two days, seminars and cryo-substitution in the next three
days, further cutting, immunolabelling, staining and viewing over the
next two days, and presentations of data on the last day. Plunge
freezing, slam freezing and cryo-TEM, cryo cutting (cryo
ultramicrotome) will be interspersed so that everyone will get a
chance to try different aspects of cryo-EM.

If you have any questions about the course please call, or better
yet, e-mail me.
Elaine

--
Dr. Elaine Humphrey
Director, Biosciences Electron Microscopy Facility
First Vice President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca
website: www.emlab.ubc.ca


From daemon Mon Apr 22 16:34:47 2002



From: KrlBer-at-aol.com
Date: Mon, 22 Apr 2002 17:27:00 EDT
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Mon Apr 22 18:06:08 2002



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Mon, 22 Apr 2002 18:57:23 -0400
Subject: Wrinkled Thicks II

Contents Retrieved from Microscopy Listserver Archives
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Yes. The temp matters. Also how you get them to Permount. I dip cooled,
stained slides in xylene, then add 1-2 drops Cytoseal and coverslip. If I
dehydrate through graded alcohols, my Spurr sections wrinkle up.

-----Original Message-----
} From: Paula Sicurello [mailto:patpxs-at-gwumc.edu]
Sent: Monday, April 22, 2002 2:25 PM
To: microscopy-at-sparc5.microscopy.com


Hi Listers,

Thanks to all who responded to my query about wrinkles. We have tried a
different mounting media but a few of the sections are still wrinkling.

These sections are flat ( I checked) before I put on the Permount, and then
they get wrinkly (like I do when I stay in the tub too long ;-) ).

My question is this: Does the temperature of the slide and mounting media
matter? My slides are still hot, the Permount or CytoSeal is room temp.
Can that cause the wrinkles? Should both be warm or room temp?

Massive frustration abounds....

I think I'll get a Shar Pei and admire his wrinkles,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Mon Apr 22 19:21:28 2002



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Mon, 22 Apr 2002 17:06:14 -0700
Subject: Re: TEM: earthquake damage to a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Brian
I am on the west coast and run a lab in a building built 30 years ago
when "earthquake code" hadn't been invented. However, when they
built this building, they put the EM Lab in the basement away from
plant operations and the elevator and they poured floating floors.
So when there was an earthquake a few months ago which shook some of
the buildings around here, I was in the SEM room and felt nothing.
The only indication I had that we had an earthquake was that the
water in the aquarium in the outer lab was sloshing around.

So one answer to your problem is to have the EMs in basement rooms
with floating floors.
Elaine


} -----------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Dr. Elaine Humphrey
Director, Biosciences Electron Microscopy Facility
First Vice President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca
website: www.emlab.ubc.ca


From daemon Mon Apr 22 22:10:00 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 22 Apr 2002 19:50:29 -0700
Subject: Re: TEM: earthquake damage to a microscope

Contents Retrieved from Microscopy Listserver Archives
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You are rich Elaine. It's my dream to have floating floor for my TEM! Sergey.

At 05:06 PM 4/22/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Apr 23 05:30:04 2002



From: e-mmunity-at-electric.net
Date:
Subject: Reporting Code Download Completion

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----- Original Message -----
} From: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
To: "Garrison, Becky" {becky.garrison-at-jax.ufl.edu}
Sent: Tuesday, April 23, 2002 11:17 AM


Sender, E-mmunity has detected virus(es) in your e-mail attachment(s).

Method: Mail


From daemon Tue Apr 23 07:52:41 2002



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Tue, 23 Apr 2002 08:44:57 -0400 (EDT)
Subject: RE: Wrinkled Thicks II

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I avoid the problem of wrinkled sections by mounting the section with
immersion oil. It works great, but be advised that the stain fades with
time (over several months).

Don

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718






From daemon Tue Apr 23 08:14:42 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 23 Apr 2002 09:13:05 -0400
Subject: Re: TEM: earthquake damage to a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;

If this is of any help, we had our FIB placed on a "floating" slab by
cutting into the concrete and isolating in that fashion. The slot which was
drilled using a wet saw which cut a 1/2" channel completely around the
instrument in an existing floor 10" thick. The open channel was filled with
a silastic flexible compound. This worked well for vibration isolation but
we are in New Jersey, unlike New York, we rarely have an Earthquake. Our
main issue wasn't from vibration but a soda machine just on the other side
of the wall. The cooling compressor's EM field was a real problem to beam
stability and my nerves. Elevators are a nightmare.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Monday, April 22, 2002 10:50 PM
To: Elaine Humphrey; Microscopy-at-sparc5.microscopy.com


You are rich Elaine. It's my dream to have floating floor for my TEM!
Sergey.

At 05:06 PM 4/22/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Apr 23 08:24:25 2002



From: charles j day :      wa5ekh-at-juno.com
Date: Tue, 23 Apr 2002 08:56:25 -0500
Subject: Amray ??? Parts service?? Manuals??

Contents Retrieved from Microscopy Listserver Archives
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I'm looking for spare parts and service-contract or corporate. Also some
routine service advice. Whenelt(oopps?sp?) cleaning, settings, etc.
Please respond direct to my email: wa5ekh-at-juno.com
jeffrey day
N. Texas Area


From daemon Tue Apr 23 08:33:58 2002



From: Fernando Capela e Silva :      fcs-at-uevora.pt
Date: Tue, 23 Apr 2002 14:28:01 +0100
Subject: Subscribe

Contents Retrieved from Microscopy Listserver Archives
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* * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laboratório de Biologia da Conservação
Departamento de Biologia
Universidade de Évora
Apartado 94
7002-554 Évora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs-at-uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm




From daemon Tue Apr 23 08:40:57 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Tue, 23 Apr 2002 08:35:30 -0500
Subject: Re: Wrinkled Thicks II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Paula,

I always cool the slides before adding the Permount. I've never had
wrinkling in this step--though I have experienced it in drying and
staining, for various reasons.

Karen Pawlowski

Paula Sicurello wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Listers,
}
} Thanks to all who responded to my query about wrinkles. We have tried a different mounting media but a few of the sections are still wrinkling.
}
} These sections are flat ( I checked) before I put on the Permount, and then they get wrinkly (like I do when I stay in the tub too long ;-) ).
}
} My question is this: Does the temperature of the slide and mounting media matter? My slides are still hot, the Permount or CytoSeal is room temp. Can that cause the wrinkles? Should both be warm or room temp?
}
} Massive frustration abounds....
}
} I think I'll get a Shar Pei and admire his wrinkles,
}
} Paula :-)
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax



From daemon Tue Apr 23 09:04:17 2002



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Tue, 23 Apr 2002 08:56:47 -0500
Subject: RE: Wrinkled Thicks II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


May I recommend:
http://www.rescueasharpei.com/html/adoption.html
?

Jeffrey A Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439
(630) 252-5594
FAX: (630) 972-4438


} ----------
} From: Paula Sicurello
} Sent: Monday, April 22, 2002 1:24 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Wrinkled Thicks II
}
} -
} Hi Listers,
}
} Thanks to all who responded to my query about wrinkles. We have tried a
} different mounting media but a few of the sections are still wrinkling.
}
} These sections are flat ( I checked) before I put on the Permount, and
} then they get wrinkly (like I do when I stay in the tub too long ;-) ).
}
} My question is this: Does the temperature of the slide and mounting media
} matter? My slides are still hot, the Permount or CytoSeal is room temp.
} Can that cause the wrinkles? Should both be warm or room temp?
}
} Massive frustration abounds....
}
} I think I'll get a Shar Pei and admire his wrinkles,
}
} Paula :-)
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax
}
}
}


From daemon Tue Apr 23 09:15:00 2002



From: Don Gantz :      Gantz-at-med-biophd.bu.edu
Date: Tue, 23 Apr 2002 10:09:07 -0400
Subject: B&W Print Processor

Contents Retrieved from Microscopy Listserver Archives
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Dear Mannie:
We've been the proud owners of a Mohr Pro 8 Processor since 1995
experiencing one relatively minor repair. Moderate use has been only for
prints and less so in the last year as we've become more digital. Please
contact directly if you have specific questions. Don

Donald Gantz
Dept. Physiology & Biophysics
Boston University School of Medicine
Boston, Massachusetts 02118
Email: Gantz-at-Biophysics.bumc.bu.edu
Phone: 617-638-4017




From daemon Tue Apr 23 10:46:48 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 23 Apr 2002 08:34:10 -0700
Subject: Re: TEM: earthquake damage to a microscope

Contents Retrieved from Microscopy Listserver Archives
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Dear Brian,
I am at the same west-coast location as Elaine, but on the fourth floor of
an even older building. When the big quake hit Seattle, I felt it plenty and
almost ran for the door. The SEMs and TEM were all fine, not even an
alignment problem. It was my nerves that suffered most.
At 10:36 AM 04/22/2002 -0400, you wrote:
}
} Over the weekend we east coasters were in for quite a surprise in that we
} had a 5.1 magnitude earthquake. The epicenter was about 25 miles from our
} laboratory. I am just curious about what sort of damage small earthquakes
} could cause to a sensitive instrument like a TEM. Are most air tables and
} anti-vibration systems sufficient to absorb most of the shock? Are any of
} my alignments in jeopardy? Is there anything that I should specifically
} check besides just using the instrument to see if it working properly? We
} have a JEOL 2010F and a Phillips CM-30. We also have a FIB tool, and my
} concern that some liquid Ga could have been knocked off into the column
} seems to be unfounded.
}
} I am sure those of you on the west coast must deal with this on a regular
} basis and can provide some insight. The major difference being that your
} buildings are probably built to a more "earthquake-proof" code than any of
} ours are.
}
} Sincerely,
}
} Brian Laughlin
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Apr 23 11:22:13 2002



From: Young, Gene (GP) :      GPYOUNG-at-dow.com
Date: Tue, 23 Apr 2002 12:00:48 -0400
Subject: RE: Carbon particle identification in Polypropylene Films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my experience, the method you describe is reasonable. It's the method
that our lab has been using for years for carbon black dispersions in
polymers.

Gene Young
Research Technologist
Analytical Sciences, Microscopy Group
The Dow Chemical Company

-----Original Message-----
} From: Kelloes, Cathy L

Hello All,

I would like some input on identifying carbon particle distribution in
polypropylene films.

Since the particles are approximately 19nm, I was thinking of using Spurr
resin and doing TEM; however, I would like to hear from some of you that
have had experience with carbon identification. Is this my best bet or
would there be a better, less time consuming method. Thanks in advance.
Cathy

Cathy Kelloes
Microscopy Technician
BP, Amoco FFBU
260 The Bluffs
Austell, GA. 30168
Phone: 770-941-1711, ext. 3255
Fax: 770-944-4745
E-mail: kelloecl-at-bp.com

This message is made of 100% recycled electrons.



From daemon Tue Apr 23 11:47:20 2002



From: Young, Gene (GP) :      GPYOUNG-at-dow.com
Date: Tue, 23 Apr 2002 11:40:17 -0500
Subject: RE: Carbon particle identification in Polypropylene Films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my experience, the method you describe is reasonable. It's the method
that our lab has been using for years for carbon black dispersions in
polymers.

Gene Young
Research Technologist
Analytical Sciences, Polymer Characterization
The Dow Chemical Company

-----Original Message-----
} From: Kelloes, Cathy L

Hello All,

I would like some input on identifying carbon particle distribution in
polypropylene films.

Since the particles are approximately 19nm, I was thinking of using Spurr
resin and doing TEM; however, I would like to hear from some of you that
have had experience with carbon identification. Is this my best bet or
would there be a better, less time consuming method. Thanks in advance.
Cathy

Cathy Kelloes
Microscopy Technician
BP, Amoco FFBU
260 The Bluffs
Austell, GA. 30168
Phone: 770-941-1711, ext. 3255
Fax: 770-944-4745
E-mail: kelloecl-at-bp.com

This message is made of 100% recycled electrons.


From daemon Tue Apr 23 16:38:33 2002



From: Shields, Vonnie :      vshields-at-towson.edu
Date: Tue, 23 Apr 2002 17:30:18 -0400
Subject: Knife assembly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,
Does anyone have a knife assembly to hold a steel knife or disposable knife blade for an LKB Historange Model 2218 microtome (microtome for paraffin sectioning) that they would be willing to sell? If so, please contact me directly via email at vshields -at-towson.edu.
Thanks.




From daemon Tue Apr 23 16:38:33 2002



From: Shields, Vonnie :      vshields-at-towson.edu
Date: Tue, 23 Apr 2002 17:31:11 -0400
Subject: Knife assembly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Listers,
Does anyone have a knife assembly to hold a steel knife or disposable knife blade for an LKB Historange Model 2218 microtome (microtome for paraffin sectioning) that they would be willing to sell? If so, please contact me directly via email at vshields -at-towson.edu.



From daemon Tue Apr 23 17:33:04 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 23 Apr 2002 17:26:24 -0500
Subject: does HRP survive osmium?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does any one know if HRP (horseradish peroxidase) survives osmium
fixation ? I know it survives aldehyde fixation. If you have a
reference, that would be extremely helpful since this is a tough
thing to focus in on with a Medline search. Thanks, Tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Apr 23 19:19:29 2002



From: zaluzec-at-microscopy.com
Date: Tue, 23 Apr 2002 20:00:41 -0500
Subject: Re: Administrivia: Ingnore "virus alert"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues:

Hello from cloudy South Korea.

Yes I have seen the virus alert message. Please ignore them. I will
try to setup to filter them from here. The are the equivalent of
a spam attack.

Nestor
Your Friendly Neighborhood SysOp




} Nestor, I have gotten an alert from
} "e-mmunity-at-electric.net-at-sparc5.microscopy.com" to
} "microscopy-at-aaem.amc.anl.gov" telling me I have a virus in my
} attachments. I haven't been posting to the list nor sending attachments
} to anyone else. Is this for real or is it SPAM?
}




From daemon Tue Apr 23 19:19:29 2002



From: Young, Gene (GP) :      GPYOUNG-at-dow.com
Date: Tue, 23 Apr 2002 19:57:40 -0500
Subject: Carbon particle identification in Polypropylene Films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my experience, the method you describe is reasonable. It's the method
that our lab has been using for years for carbon black dispersions in
polymers.

Gene Young
Research Technologist
Analytical Sciences, Microscopy Group
The Dow Chemical Company


-----Original Message-----
} From: Kelloes, Cathy L [mailto:KELLOECL-at-bp.com]
Sent: Monday, April 22, 2002 6:40 AM
To: MSA (E-mail)


Hello All,

I would like some input on identifying carbon particle distribution in
polypropylene films.

Since the particles are approximately 19nm, I was thinking of using Spurr
resin and doing TEM; however, I would like to hear from some of you that
have had experience with carbon identification. Is this my best bet or
would there be a better, less time consuming method. Thanks in advance.
Cathy

Cathy Kelloes
Microscopy Technician
BP, Amoco FFBU
260 The Bluffs
Austell, GA. 30168
Phone: 770-941-1711, ext. 3255
Fax: 770-944-4745
E-mail: kelloecl-at-bp.com

This message is made of 100% recycled electrons.


From daemon Tue Apr 23 22:28:41 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 23 Apr 2002 20:19:25 -0700
Subject: Re: B&W print processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Take a look at Jobo and Nova. Both make nice
print processors. There was another, perhaps better,
maker but I sense that they are gone(?).

There are places that sell processors used, at good
prices. But they units are iffy respective to availability.

Disclaimer: Since I am all-digital, I have no vested interest
in any print processor maker. Never had one before, either.

gary g.

At 05:41 AM 4/22/2002, you wrote:

} I will soon be replacing my current B&W print processor in my darkroom. I
} know that there used to be several on the market but I have only been able to
} } locate one,the MohrPro. Any feed back on this processor or any other one
} currently
} available along with estimated costs would be greatly appreciated.
}
} Mannie Steglich



From daemon Wed Apr 24 09:06:08 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 24 Apr 2002 09:48:58 -0400
Subject: Re: does HRP survive osmium?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I seem to recall a paper in the late '70's/early '80's reporting that
peroxidase acitivity in RBC's and other cells survived routine glut-OsO4
fixation and subsequent embedding. I will see if I can find the actual
reference later today.

Tom Phillips wrote:

} Does any one know if HRP (horseradish peroxidase) survives osmium
} fixation ? I know it survives aldehyde fixation. If you have a
} reference, that would be extremely helpful since this is a tough
} thing to focus in on with a Medline search. Thanks, Tom
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Apr 24 09:06:08 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 24 Apr 2002 09:56:48 -0500
Subject: HRP + osmium clarification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to clarify my original query. My question is not whether the
HRP reaction product survives osmium but whether the enzyme does. I
am aware of the many papers, including my own, that label with HRP or
a conjugate prior to aldehyde fixation, that then perform the
reaction with DAB or similar substrate followed by osmication and
embedding. Osmication intensifies the reaction product. What I am
wondering is whether, following in vivo labeling with HRP, a tissue
can be fixed in osmium and then the substrate added to generate the
deposit. Thanks for any insights. Tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Apr 24 09:06:08 2002



From: Earl Weltmer :      earlw-at-sbcglobal.net
Date: Wed, 24 Apr 2002 06:55:04 -0700
Subject: email virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Is it just me or have any (or all) been the recipient of email viruses
especially from Asia.

Earl





From daemon Wed Apr 24 11:41:51 2002



From: McCarthy, Deborah :      DMccar-at-lsuhsc.edu
Date: Wed, 24 Apr 2002 11:29:00 -0500
Subject: HRP surviving osmium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is in response to Thomas Phillips question about HRP surviving
osmication. Ten years ago I worked with an electrophysiologist who would
inject HRP into neurons, perfuse the animal with para/glut. mix and then it
was my job to vibratome the chunk of brain, put it into trays and then react
the tissue for HRP. I just remembered we would let the brain sink in
buffered sucrose overnight in the refrig.prior to the vibratoming. If
memory serves me right we would use Vector Labs' kit ( you can also make
your own solutions)for development of the HRP and then I would continue
tissue processing for EM, osmication,dehydration ,and then infiltration.
You must rinse the tissue very well after fixation with the buffer system
used in your fixative prior to reacting the tissue for HRP. Hope this helps
a little.

Deborah McCarthy
LSUHSC
Dept. Pathology
dmccar-at-lsuhsc.edu



From daemon Wed Apr 24 13:09:03 2002



From: Ruth Yamawaki :      Ryamawaki-at-cmexchange.stanford.edu
Date: Wed, 24 Apr 2002 11:02:12 -0700
Subject: EM technician position at Stanford University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

We have an job opening for an EM technician here at Stanford University in
California. For more information about this position and Stanford
University please check out
http://hrweb.stanford.edu/jobs/openings/display.cgi?Job_Req=001149&JFam=NIL

Life Science Research Assistant (#001149). Range: 2P1. Posted: 04/24/2002.
Description
The Department of Comparative Medicine is seeking a Life Science Research
Assistant to be responsible for electron microscopy in a neuroscience
laboratory. Duties will include: serial section examination using electron
microscope and analysis of electron micrographs (including 3-dimensional
reconstruction), preparing tissue for electron microscopic examination
(including post-embedding immunocytochemistry), ultrathin serial sectioning
and interpreting results and data. This position will also be expected to
implement new methods to improve laboratory procedures and maximize
productivity as well as develop negatives and supervise a part-time employee
who will make prints. This position will also be responsible for training
other personnel in photographic and electron microscopic techniques.
QUALIFICATIONS: Bachelor's degree in biology or equivalent area. Advanced
training in electron microscopy is desirable. Candidate must have at least
1-2 years of related work experience, 2-3 years is desired.

Please send resumes to:

Dr. Paul Buckmaster
Stanford University Medical School
Department of Comparative Medicine
300 Pasteur Drive
Edwards Building Room R102
Stanford, CA 04305-5330

Phone: (650) 498-4774
Fax: (650) 498-6259


Thanks,

Ruth

*******************************************
Ruth Yamawaki
Stanford University Medical Center
Department of Comparative Medicine
Edwards Building, Room R102
Stanford CA 94305-5330

phone (650) 723-3457
fax (650) 498-6259




From daemon Wed Apr 24 13:29:51 2002



From: Samuel Purdy :      spurdy52-at-mac.com
Date: Wed, 24 Apr 2002 17:22:35 -0500
Subject: Expiration Dates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

Some time ago, there was a thread about the performance of different
kinds of BSE detector for SEM.

I had earlier used a superb scintillator/PMT BSE detector designed
and built by Rudolf Autrata. This detector uses a single-crystal of
Yttrium-aluminum garnet (YAG), mounted concentric to the beam and
just below the polepiece of the objective lens, to convert BSE into
light. It also employs a very sophisticated set of reflective and
antireflective coatings to convey as much as possible of the light
produced to the PMT.

Using it, Ralph Albrecht and I were able to record useful BSE images
at beam voltages below 2 kv, in the Hitachi S-900. This was possible
without any positive bias voltage applied to the detector to increase
the impact energy of the incoming BSE.

Compared to semiconductor BSE detectors, I would say that the YAG has
better low kV performance and lower "dark cuyrrent". Compared with
other scintillators/PMT BSE detectors, the YAG has more radiation
resistance (longer life) than plastic scintillators and less phosphor
noise than detectors using powdered phosphors.

I didn't respond earlier because I first had to check with Prof.
Autrata to be sure that the detectors were still available. After
some delays caused by computer viruses, I attach his reply and
contact information below.

I have no financial interest in this detector or the company that
makes it: just a satisfied user.

Jim P.

________________________



Randy:
I can't address your problem on expiration dates directly. However, in
my metallography laboratory, stable inorganic chemicals in their original
bottles are held indefinitely. Stable made up etching solutions using
organic solvnts are discarded after 6 onths even though they are stored in
tight bottles. There appears to be a slow escape of solvent that gradually
increases the concentration of the solution. Aqueos solutions appar not to
evaporate as rapidly but we discard them after 6 months anyhow. Unstable
solutions are dumed immediately after use and made up fresh for each
pplication.

Sam Purdy
Ntional Steel Technical Center (Retired)
spurdy52-at-earthlink.net


From daemon Wed Apr 24 16:36:05 2002



From: Fernando Capela e Silva :      fcs-at-uevora.pt
Date: Wed, 24 Apr 2002 17:20:12 -0500
Subject: SEM and TEM for bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like someone send me a general bone preparation scheme for SEM and
TEM.

With compliments
* * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laboratório de Biologia da Conservação
Departamento de Biologia
Universidade de Évora
Apartado 94
7002-554 Évora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs-at-uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm


From daemon Wed Apr 24 16:36:05 2002



From: Fernando Capela e Silva :      fcs-at-uevora.pt
Date: Wed, 24 Apr 2002 17:20:12 -0500
Subject: SEM and TEM for bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like someone send me a general bone preparation scheme for SEM and
TEM.

With compliments
* * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laboratório de Biologia da Conservação
Departamento de Biologia
Universidade de Évora
Apartado 94
7002-554 Évora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs-at-uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm


From daemon Wed Apr 24 16:36:05 2002



From: Samuel Purdy :      spurdy52-at-mac.com
Date: Wed, 24 Apr 2002 17:22:35 -0500
Subject: Expiration Dates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy:
I can't address your problem on expiration dates directly. However, in
my metallography laboratory, stable inorganic chemicals in their original
bottles are held indefinitely. Stable made up etching solutions using
organic solvnts are discarded after 6 onths even though they are stored in
tight bottles. There appears to be a slow escape of solvent that gradually
increases the concentration of the solution. Aqueos solutions appar not to
evaporate as rapidly but we discard them after 6 months anyhow. Unstable
solutions are dumed immediately after use and made up fresh for each
pplication.

Sam Purdy
Ntional Steel Technical Center (Retired)
spurdy52-at-earthlink.net


From daemon Thu Apr 25 04:02:08 2002



From: Anne-Mette Heie =?iso-8859-1?Q?Kj=E6r?= :      ahk-at-topsoe.dk
Date: Thu, 25 Apr 2002 10:51:10 +0200
Subject: Amorphous Ge-film for high resolution TEM-calibration.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

Could someone help me finding where to buy a tem-grid with a film of
amorphous Germanium?

Thank you in advance,

Anne-Mette Heie Kjaer



From daemon Thu Apr 25 04:19:45 2002



From: Fernando Capela e Silva :      fcs-at-uevora.pt
Date: Thu, 25 Apr 2002 10:15:20 +0100
Subject: Bone SEM an TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like someone send me a protocal for processing bone for SEM and TEM.

With compliments
* * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laboratório de Biologia da Conservação
Departamento de Biologia
Universidade de Évora
Apartado 94
7002-554 Évora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs-at-uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm




From daemon Thu Apr 25 06:26:50 2002



From: Alexander Black :      alexander.black-at-nuigalway.ie
Date: Thu, 25 Apr 2002 12:18:03 +0100
Subject: Microscopical Society of Ireland - 26th Annual Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopical Society of Ireland - 26th Annual Symposium
Wednesday 28th August to Friday 30th August 2002
to be held at
National University of Ireland, Galway

CALL FOR ABSTRACTS

Abstracts submissions for either poster or oral communications in any
area of research involving microscopy are invited.

The Society endeavours to foster an interest in the science and practice
of microscopy and provides a forum for academic and technical dialogue
in matters relating to microscopy on the entire Island of Ireland and
beyond.

The Annual Symposium is conducted in a relaxed yet semi-formal
environment, and proves the ideal platform for students, especially
those who have yet to experience a scientific meeting for the first
time.

Student prizes are awarded for the best student oral presentation and
best student poster presentation in both the Biological and Material
Sciences.

Registration for the symposium includes membership of the MSI for one
year. Members are reminded that student members may make application to
the committee for Student Travel Bursaries.

Information will be updated as available at www.nuigalway.ie/msi

Queries may be sent via email to: MSI-at-nuigalway.ie



From daemon Thu Apr 25 07:37:14 2002



From: support-at-clubobscene.com
Date: Thu Apr 25 05:29:38 2002
Subject: Re: Peek behind the scenes at Paws Inc., the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America






Greetings from the Customer Service Department.

We want to help you as quickly as we can so we are experimenting with an
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Q: WHY WON'T MY PASSWORD WORK?

1) Make sure you are trying to access the correct site. If you enter your
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The vast majority of the thousands of live and pre-recorded feeds WE OFFER
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} CjwvSFRNTD4NCg0K
} --P92x3U8520bNj6C942HX607KvM5--
}


From daemon Thu Apr 25 07:54:53 2002



From: Carole Cooper :      carole_a_cooper-at-hotmail.com
Date: Thu, 25 Apr 2002 15:39:18 +0000
Subject: TEM-polymer thinning

Contents Retrieved from Microscopy Listserver Archives
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for {microscopy-at-aaem.amc.anl.gov} ; Thu, 25 Apr 2002 07:46:21 -0500 (CDT)


Incident Information:-

Originator: "support-at-clubobscene.com"-at-sparc5.microscopy.com
Recipients: microscopy {microscopy-at-aaem.amc.anl.gov}


Hi,
Does anyone know of a way to thin a central section of a polymer composite
specimen. I won't be using grids as my specimen is larger. Specifically I
have a specimen 8.5 mm x 2.5 mm and 0.1 mm thick. I need the thickness at
the edges of the specimen so that I can handle it.

I need the central region to be electron transparent. Ion thinning and
dimplers seem to be set up for metals and ceramics. Has anyone used these
methods for polymers? Any other good suggestions?

many thanks,
Carole




_________________________________________________________________
Send and receive Hotmail on your mobile device: http://mobile.msn.com



From daemon Thu Apr 25 14:04:13 2002



From: ANTIGEN_EMAIL :      ANTIGEN_EMAIL-at-uamail.albany.edu
Date: Thu, 25 Apr 2002 14:55:46 -0400
Subject: Antigen found Exploit-MIME.gen (McAfee4,CA(Vet)) virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Antigen for Exchange found Unknown infected with Exploit-MIME.gen
(McAfee4,CA(Vet)) virus.
The file is currently Removed. The message, "Re: Peek behind the scenes at
Paws Inc., the", was
sent from support-at-clubobscene.com-at-sparc5.microscopy.com and was discovered
in IMC Queues\Inbound
located at UAlbany/ADM/EMAIL.


From daemon Thu Apr 25 14:04:13 2002



From: ANTIGEN_EMAIL :      ANTIGEN_EMAIL-at-uamail.albany.edu
Date: Thu, 25 Apr 2002 14:55:47 -0400
Subject: Antigen found HTML.MimeExploit (CA(Vet)) virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Antigen for Exchange found Unknown infected with HTML.MimeExploit (CA(Vet))
virus.
The file is currently Removed. The message, "Re: Peek behind the scenes at
Paws Inc., the", was
sent from support-at-clubobscene.com-at-sparc5.microscopy.com and was discovered
in IMC Queues\Inbound
located at UAlbany/ADM/EMAIL.


From daemon Thu Apr 25 15:04:13 2002



From: ANTIGEN_INRS-IAF-EXCHG :      ANTIGEN_INRS-IAF-EXCHG-at-inrs-iaf.uquebec.ca
Date: Thu, 25 Apr 2002 20:10:18 -0400
Subject: Antigen found Exploit-MIME.gen (McAfee4) virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Incident Information:-

Originator: "support-at-clubobscene.com"-at-sparc5.microscopy.com
Recipients: microscopy {microscopy-at-aaem.amc.anl.gov}


Incident Information:-

Originator: "support-at-clubobscene.com"-at-sparc5.microscopy.com
Recipients: microscopy {microscopy-at-aaem.amc.anl.gov} ,
sdesmercieres-at-ato.com


Antigen for Exchange found Unknown infected with Exploit-MIME.gen (McAfee4)
virus.
The file is currently Removed. The message, "Re: Peek behind the scenes at
Paws Inc., the", was
sent from support-at-clubobscene.com-at-sparc5.microscopy.com and was discovered
in IMC Queues\Inbound
located at IRNS/INRS-IAF/INRS-IAF-EXCHG.


From daemon Fri Apr 26 04:37:17 2002



From: kate_francis-at-e-mailanywhere.com
Date: Fri, 26 Apr 2002 10:09:35 -0100
Subject: .BIZ and .INFO available here

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


.BIZ and .INFO Available Here

Dear sir/madam,

The Internet Corporation for Assigned Names and Numbers [ICANN] has recently approved the addition of new extensions to the internet domain space. The new domain names will offer much-needed competition in the domain market. Consumers will be able to choose from the following new extensions: .biz, .info, and .name

The new top-level domain names are now available to the general public at the following address: http://www.interniconline.com. Priced at only $24.95, the best names are expected to be snapped up quickly so don't hesitate.

Sincerely,

Internic Online
http://www.interniconline.com
7650UkUB9-238JpPG3930jBok5-220Mjsn7582ZnGl39


From daemon Fri Apr 26 06:17:46 2002



From: schnaith-at-uni-hohenheim.de
Date: Fri, 26 Apr 2002 13:09:40 +0200 (CEST)
Subject: compostion of a medium to induce pollen tube growth of BARLEY?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listeners!


Does anybody know the compostion (which sugar, nutrients, phytohormons and gel
in which concentration) of a medium to induce pollen tube growth of BARLEY (H.
vulgare, spontaneum, bulbosum)?

Does light and temperature play a big role in inducing?

Could anybody refer me to some papers (especially BARLEY / selfing species)?


Thanks, and best wishes!

Florian (MA-student of agricultural sciences)





From daemon Fri Apr 26 06:25:05 2002



From: schnaith-at-uni-hohenheim.de
Date: Fri, 26 Apr 2002 13:19:27 +0200 (CEST)
Subject: Compostion of a medium to induce pollen tube growth of BARLEY?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listeners!


Does anybody know the compostion (which sugar, nutrients, phytohormons and gel
in which concentration) of a medium to induce pollen tube growth of BARLEY (H.
vulgare, spontaneum, bulbosum)?

Does light and temperature play a big role in inducing?

Could anybody refer me to some papers (especially BARLEY / selfing species)?


Thanks, and best wishes!

Florian (MA-student of agricultural sciences)


From daemon Fri Apr 26 09:03:55 2002



From: F. J. Moura Nunes :      mouranunes-at-ipolisboa.min-saude.pt
Date: Fri, 26 Apr 2002 14:52:34 +0100
Subject: SEM and TEM for bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Caro Capela e Silva
Pra TEM do osso sugiro-lhe o descrito por exemplo em Glauert M e Lewis PR -
Biological Specimen Preparation for TEM, Portland Press, London 1998,p308-9,
ou seja:
1 - fixar em glut standard, em fatias de { 3mm
2 - Mergulhar em EDTA-dissódico a 10%, pH 7.4 ajustado com NaOH 1N, durante
1 a 3 semanas (é mesmo durante muito tempo!!) à TA. É conveniente usar
soluto fresco, mudar todos os dias e agitar pelo menos 2 x dia.
3 - Vai-se controlando o grau de descalcificação um tanto a olho...
4 - Lavar com o tampão de lavagem, cortar em blocos pequenos, fixar em ósmio
e seguir a técnica de rotina.

Já fizemos algumas inclusões de osso humano e as coisas correram bem. Pelo
sim pelo não fomos muito cuidadosos no uso da faca de diamente para cortar
os finos. Só depois de garantir que nos semifinos não havia sombras de
czonas calcificadas é que avançámos.
Boa sorte
J.F. Moura Nunes
Lab. Electron Microscopy
Portuguese Cancer Institute
Lisbon, Portugal
----- Original Message -----
} From: "Fernando Capela e Silva" {fcs-at-uevora.pt}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, April 24, 2002 11:20 PM


I would like someone send me a general bone preparation scheme for SEM and
TEM.

With compliments
* * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laboratório de Biologia da Conservação
Departamento de Biologia
Universidade de Évora
Apartado 94
7002-554 Évora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs-at-uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm





From daemon Fri Apr 26 10:18:39 2002



From: PESTOEM-at-aol.com
Date: Fri, 26 Apr 2002 11:10:20 EDT
Subject: Earl Weltmers E-Mail address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to send an E-Mail to Earl Weltmer of Scanservice Corp.
but the {eweltmer-at-home.com} keeps coming back.
Thank you, Peter Stolzenberg , Pesto Inc.


From daemon Fri Apr 26 10:24:45 2002



From: Frida.Maiers-at-co.hennepin.mn.us
Date: Fri, 26 Apr 2002 10:15:41 -0500
Subject: TEM: depolymerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a procedure to remove epoxy resin from polymerized
blocks. The goal would be to eventually stain the tissue previously
embedded for light microscopy and immunofluorescent techniques. Is this
possible and would there be a reference that explains this procedure?
Thanks,
Frida Maiers




From daemon Fri Apr 26 10:56:47 2002



From: DrJohnRuss-at-aol.com
Date: Fri, 26 Apr 2002 11:44:45 EDT
Subject: Photoshop-7 compatible free plug-ins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Several freely-downloadable Photoshop-compatible plug-ins have been offered
to readers of this group before: adaptive equalization (enhances the
visibility of fine detail), an improved custom convolution filter (larger
kernels, real values, and autoscaling), and a magnification bar drawing
routine (particularly useful for microscopists). New versions of these are
now compatible with Photoshop 7 (as well as versions 3, 4, 5 and 6) and with
Mac OSX and Windows XP (as well as OS 8 and 9 and Win 95/98/ME/NT/2K). All
work with both 8- and 16-bit per channel gray scale and RGB color images.
They can be downloaded from {http://ReindeerGraphics.com/free.html} (and you
can also get information there on the full set of plug-ins for image
processing and analysis in The Image Processing Tool Kit and Fovea Pro).


From daemon Fri Apr 26 11:43:24 2002



From: Paul Midgley :      pam33-at-cus.cam.ac.uk
Date: Fri, 26 Apr 2002 17:37:11 +0100
Subject: TEM Post-doc position Univ of Cambridge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



UNIVERSITY OF CAMBRIDGE

Post-Doctoral Research Assistant

The Development of Electron Tomography (3D-TEM) for the Physical Sciences

DEPARTMENT OF MATERIALS SCIENCE AND METALLURGY

A Post-Doctoral Research Assistant position is available immediately to
pursue a programme of research into the development of electron tomography
for the physical sciences with a particular emphasis on the 3-dimensional
structure and composition of nanoscale devices and catalysts. The successful
applicant will join a small team at Cambridge developing electron tomography
using STEM HAADF imaging and core-loss energy-filtered TEM. The research is
in conjunction with the Department of Chemistry in Cambridge and the Royal
Institution in London. More information about the research can be found at:

http://www-hrem.msm.cam.ac.uk/research/CETP/electron_tomography.html

Applicants should have experience of transmission electron microscopy and
image processing with a background in an experimental physical science.

The position is funded by the EPSRC, available immediately and for 2 years
in the first instance. The starting salary will be on the University RA1A
scale (£17,626 - £26,491)
depending on experience.

Informal enquiries can be made to Dr Paul Midgley:
tel. +44 1223 334561 or e-mail pam33-at-cam.ac.uk.

Applications should be sent to Dr P.A. Midgley, Department of Materials
Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge,
CB2 3QZ, U.K. and should include
a full CV with the names and addresses of two referees.

Closing date: 31 May 2002.
___________________________________________________________
Dr Paul A. Midgley
University of Cambridge AND Peterhouse
Dept of Materials Science Cambridge
& Metallurgy, Pembroke Street CB2 1RD
Cambridge CB2 3QZ U.K. U.K.

Tel: (+44)-(0)1223-334561 Tel: (+44)-(0)1223-766503
Fax: (+44)-(0)1223-334567 Fax: (+44)-(0)1223-337578
___________________________________________________________



From daemon Fri Apr 26 17:26:02 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 26 Apr 2002 15:10:13 -0700
Subject: SEM: Can I look at Hg?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Can I look at metallic mercury in a conventional SEM?

Here's the set up:

A researcher makes electrodes of 'glassy carbon'. He adds a membrane of
Nafion (teflon like, maybe 200 nm thick, makes like a holey formvar
covering) to the surface.

Then he plates metallic mercury through the pores of the membrane onto the
electrode.

He wants to know if the mercury fills up the pores and spills over onto the
top of the membrane or not.

We have a conventional SEM. I don't know if mercury will hold up in the
chamber at normal SEM pressures. Anyone know for sure?

If this won't work, any ideas for a Plan B?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Apr 26 18:36:51 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 26 Apr 2002 15:54:42 -0700
Subject: QX3 "toy" microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Some of you have experimented with the Intel QX3 "toy" microscope, which is
now being marketed to teachers with more software (and a higher price). If
you're a real geek, a link to its CPiA command structre is given at
http://slashdot.org/science/02/04/21/1719259.shtml?tid=137 . The same URL
has an under-$50 ordering source. If you make the thing do new tricks,
please let us know!

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Apr 26 22:09:01 2002



From: PfungosaKR-at-aol.com
Date: Fri, 26 Apr 2002 23:01:07 EDT
Subject: QX3 "toy" microscope and new tricks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings all,
I've used the microscope extensively, especially with elementary-aged
students. The final image on the monitor is greatly improved if you use
high-quality microscope slides.


From daemon Sat Apr 27 00:26:03 2002



From: ssgrr2002s-at-rti7020.etf.bg.ac.yu
Date: Sat, 27 Apr 2002 07:16:32 +0200
Subject: Call for papers for the SSGRR 2002s Conference in L`Aquila near Rome, Italy (Jul 29 - Aug 4 2002.)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopy,

I have been appointed to serve as the General Chair of the Summer 2002
edition of the SSGRR series of international conferences,
and I would like to extend a special invitation to you.

The SSGRR-2002S (Summer) conference on
"Infrastructure for e-Business, e-Education, e-Science, and e-Medicine"
takes place in SSGRR (Scuola Superiore G. Reiss Romoli), the delux
congress and educational center of the Telecom Italia Group of companies.

This is in L'Aquila near Rome, Italy, from July 29
(Monday) at 5pm (start of the Grand Opening) till August 4 (Sunday) at
10am (departure of busses to the Rome airport Fiumicino and the
railway station Tiburtina).

Most of the past participants beleive that this was one of the most
interesting, most useful, and definitely THE most hospitable conference
they ever attended.

The SSGRR-2002S will be open by Jerome Friedman from MIT
(laureate of the NOBEL PRIZE) and Travor Gruen-Kennedy of Citrix
(listed by some sources, together with Bill Gates,
as one of the world's TOP-25 contributors to the
development of the Internet).

For details, see the WWW site of the conference
(www.ssgrr.it/en/ssgrr2002s/index.htm).
Among other things, this WWW site also includes the full-blown version
of the invitation letter-contract, with all relevant details
(www.ssgrr.it/en/ssgrr2002s/invitation.htm).

The soft deadline for you to decide if you are coming is May 25,
2002 (in the worst case you should respond before May 31st). By that
date the place for you is unconditionally reserved. After
that date, you will be accepted to the conference only if the existing
240 places are not filled.

Before May 25, 2002, please send only the following: (a) TITLE,
(b) AUTHORS, (c) AFFILIATION, (d) ABSTRACT, and (e) STATEMENT THAT YOU
WILL COME 100% (answers like "maybe" will be treated as NO answer from
you). The full paper is due on June 10, 2002.

The early registration price for the 6-day stay at SSGRR is EURO1200
(if you represent an institution) or EURO1440 (if you come as an
individual). Coming without a paper costs you extra EURO600 or EURO720,
respectively. Deadline for the early registration is June 30, 2002.

If you come with an accompanying person, the early registration extra
cost is EURO300, for 6 days of bed and breakfast, in an external hotel
(please note that the best external hotels are 4-star, and not nearly
as comfortable as the accomodation in the SSGRR complex). The SSGRR
complex includes only single-bed rooms, and therefore available only
to those who come without an accompanying person.

If you have any questions, please check the WWW site of the conference
and especially the part entitled FAQ(Frequently Asked Questions). If
you still have questions or there is something that we can do for you,
please write to Organizing Committee
at ssgrr2002s-at-rti7020.etf.bg.ac.yu (preferred)
or if absolutely neccessary, to myself directly (vm-at-etf.bg.ac.yu).

Sincerely yours,

Professor V. M. Milutinovic,
General Chair of the SSGRR-2002S
(galeb.etf.bg.ac.yu/~vm/)

P.S. No matter if you will attend the SSGRR-2002S conference or not,
please let us know if you like to be invited to the Winter edition
of the year 2003 (SSGRR-2003W) to be held in the same place
from January 6, 2003 at 5pm till January 12, 2003 at 10am.
Shall we reinvite you?

Of course, if you wish not to receive again information about
the SSGRR conferences, please let us know, and we will remove
your name from our list.

IMPORTANT DETAILS:

1. We invite participants from three groups:
A: Researchers from the list of the most referenced scientists
B: VIPs of succesfull high-tech companies
C: Young talent (according to the criteria
of the Organizing Committee)
We try to maximaze the synergistic interraction among these 3 groups.

2. Your presentation is 25 minutes, plus 5 minutes for discussion
and the change of speakers.

3. The author of the LAST paper in the session is the session chairman,
so he/she is motivated to respect the timing.
The slots of the non-show-up papers are to be used for
extra discussions. Moving of presentation slots is NOT permitted.

4. Timing of the session is given on the WWW site of the conference.

5. More information on the SSGRR center is given on the WWW site
of the conference.

6. Transportation related information,
on July 29 from Tiburtina station in Rome to SSGRR in L'Aquila,
and on August 4 from L'Aquila to Tiburtina station and Fiumicino
airport is given in the conference WWW site (pay attention to FAQ).

7. Details of the food schedule, social program, and all other
relevant details are also given on the WWW site of the conference.



From daemon Sat Apr 27 03:58:28 2002



From: Krzysztof Herman :      kherman-at-labsoft.com.pl
Date: Sat, 27 Apr 2002 10:51:08 +0200
Subject: QX3 Intel toy...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello

I am using this toy since over year. I added simple adaptor-stand and use it to center
filaments, and to document in service some status of micromechanical items (bent sample
holders in TEM, preview TEM grids to locate sections on non-indexed grids etc.).
It has with standard software bundle a driver-shell which is recognized by other graphical
softwares (I use Microsoft Picture It Express - purchased as a bonus with pack of Pelikan
photo paper) which have much more "normal" user interface, more resolution range for
stored pictures than the child-toy looking original intel poduct IntelPlay.
In general it is very good and nice idea to combine simple magnifying tube and cheap
internet CCD camera (that.s how it is done).
If the camera and magnifying glasses would be of a bit higher class - say - like todays
top level internet cameras - it could be really valuable produst for even some
professional low mag applications.

greetings

Krzysztof Herman
FEI EO - Service
ul.Ba¿ancia 45A, 02-892 Warszawa, POLAND
tel/fx: (+48 22)6449753, 6449750
mobile: (+48 601)307456
www.labsoft.com.pl



From daemon Sat Apr 27 04:56:09 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 27 Apr 2002 05:45:29 -0500
Subject: amorphous films of germanium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Anne-Mette Heie Kjaer wrote:
====================================================
} } Could someone help me finding where to buy a tem-grid with a film of
} amorphous
} } Germanium?
====================================================
I believe this would be difficult to make. I am not saying impossible, just
difficult. There would be some big-time safety concerns as well, so I have
been told.

But if your interest is mainly because you want something completely
structureless and featureless, yet something that is more robust than a
polymer film, what about the silicon nitride membrane window grids offered
by SPI Supplies, and described on URL
http://www.2spi.com/catalog/instruments/silicon-nitride.shtml

Compared to a polymer film, the nitride membrane is indestructable.

Chuck

Disclaimer: SPI Supplies offers a family of silicon nitride membrane window
grid products and therefore we would like to see more people using them!

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sat Apr 27 18:54:32 2002



From: Alan Davis :      adavis-at-saipan.com
Date: Sun, 28 Apr 2002 09:39:39 +1000
Subject: Re: QX3 Intel toy...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On slashdot.org is an article (link below) with numerous links on the QX3, including modifications, webpages. The ensuing discussion leads to a number of other links.
The Link: http://slashdot.org/science/02/04/21/1719259.shtml?tid=137

I have seen, but been unable to relocate, a web page about converting the QX3 to a credible microscope camera. The CPiA is based on CMOS technology; it is not a CCD.

There is still the Kodak MDS-100 microscope camera for only a bit more, on ebay for less than 100.00, a CCD camea with a higher pixel count.

I want both.

Alan Davis
Marianas High School, Saipan



On Sat, 27 Apr 2002 10:51:08 +0200
"Krzysztof Herman" {kherman-at-labsoft.com.pl} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello
}
} I am using this toy since over year. I added simple adaptor-stand and use it to center
} filaments, and to document in service some status of micromechanical items (bent sample
} holders in TEM, preview TEM grids to locate sections on non-indexed grids etc.).
} It has with standard software bundle a driver-shell which is recognized by other graphical
} softwares (I use Microsoft Picture It Express - purchased as a bonus with pack of Pelikan
} photo paper) which have much more "normal" user interface, more resolution range for
} stored pictures than the child-toy looking original intel poduct IntelPlay.
} In general it is very good and nice idea to combine simple magnifying tube and cheap
} internet CCD camera (that.s how it is done).
} If the camera and magnifying glasses would be of a bit higher class - say - like todays
} top level internet cameras - it could be really valuable produst for even some
} professional low mag applications.
}
} greetings
}
} Krzysztof Herman
} FEI EO - Service
} ul.Ba¿ancia 45A, 02-892 Warszawa, POLAND
} tel/fx: (+48 22)6449753, 6449750
} mobile: (+48 601)307456
} www.labsoft.com.pl
}
}


--
adavis-at-saipan.com 1-670-322-6580
Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI

I have steadily endeavored to keep my mind free, so as to give up any
hypothesis, however much beloved -- and I cannot resist forming one
on every subject -- as soon as facts are shown to be opposed to it.
-- Charles Darwin (1809-1882)






From daemon Sat Apr 27 23:08:43 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Sat, 27 Apr 2002 22:26:33 -0500
Subject: Re: TEM: depolymerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Frida,

How's Minnesota? Too bad about the snow last week. Typical MN Spring.
I'm a Minnesota Alumni and I still have family there. :)

There is a solution of sodium methoxide that should work on your cross
sections. It is made by making a saturated solution of sodium hydroxide
in methanol. Add 5 to 10 grams NaOH pellets to 50 ml methanol in a
dark glass bottle. Let it stand for two to three weeks before using it.
The longer it stands, the stronger it gets, so you may want to dilute it
more as it ages. Typically this solution is diluted in methanol 1
part solution to 3 parts methanol. Etching times differ according to
age of solution and thickness of the section. I can't find the original
reference for it, but S.R. Shi, et al used a version of it in the 90's
for
etching celloidin sections for immunohistochemistry. (Sorry, the web is
down here right now, or I'd give you the complete references.) Maybe
someone else knows the refs. and will respond.

The solution is a nasty one to have around, but it will work. I just put
a drop or two on the slide. Be careful not to over etch. Once etched,
I rinse the sections thoroughly with distilled water and continue with
the stain technique.

Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas, Dallas, Texas


"Frida.Maiers-at-co.hennepin.mn.us"-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are looking for a procedure to remove epoxy resin from polymerized
} blocks. The goal would be to eventually stain the tissue previously
} embedded for light microscopy and immunofluorescent techniques. Is this
} possible and would there be a reference that explains this procedure?
} Thanks,
} Frida Maiers



From daemon Sun Apr 28 17:14:44 2002



From: thurston e herricks :      thurst0n-at-u.washington.edu
Date: Sun, 28 Apr 2002 15:02:41 -0700 (PDT)
Subject: Method for measuring Magnetic fields

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

What are standard methods for measuring magnetic fields in a room?
We need to know find out if a room meets specifications for installing a
TEM. We have a gauss meter. However, we are unsure of a method to
compare different rooms to one anouther for choosing the best location
for the TEM.

Thank you for your time

Take care

Thurston Herricks



From daemon Sun Apr 28 20:02:36 2002



From: norica.richardson-at-webmail.co.za
Date: Sun, 28 Apr 2002 20:52:26 +0400
Subject: new domain extensions are now affordable

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Price Discount for New TLD Extensions

Dear sir/madam,

The Internet Corporation for Assigned Names and Numbers [ICANN] has recently approved the addition of new extensions to the internet domain space. The new domain names will offer much-needed competition in the domain market. Consumers will be able to choose from the following new extensions: .biz, .info, and .name

The new top-level domain names are now available to the general public at the following address: http://www.interniconline.com. Priced at only $24.95, the best names are expected to be snapped up quickly so don't hesitate.

Sincerely,

Internic Online
http://www.interniconline.com
2397HaSx3-190SEIQ2627EPUG9-602BEhj0266nPVcl40



From daemon Mon Apr 29 02:40:07 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 29 Apr 2002 08:33:13 +0100 (GMT Daylight Time)
Subject: Re: SEM: Can I look at Hg?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jon,

According to my copy of 'Leybold Vacuum Handbook' the
vapour pressure of Hg at 10-5 torr is 245K rising to 318K
at 10-2 Torr (sorry about the units - it's an old book).

With the common use of Al in EMs I would not reccommend
looking at Hg unless you can use an environmental SEM or a
cold stage.

Regards,
Ron


On Fri, 26 Apr 2002 15:10:13 -0700 Jon Krupp
{jmkrupp-at-cats.ucsc.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Can I look at metallic mercury in a conventional SEM?
}
} Here's the set up:
}
} A researcher makes electrodes of 'glassy carbon'. He adds a membrane of
} Nafion (teflon like, maybe 200 nm thick, makes like a holey formvar
} covering) to the surface.
}
} Then he plates metallic mercury through the pores of the membrane onto the
} electrode.
}
} He wants to know if the mercury fills up the pores and spills over onto the
} top of the membrane or not.
}
} We have a conventional SEM. I don't know if mercury will hold up in the
} chamber at normal SEM pressures. Anyone know for sure?
}
} If this won't work, any ideas for a Plan B?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Mon Apr 29 03:05:34 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 29 Apr 2002 09:00:54 +0100 (GMT Daylight Time)
Subject: Re: Method for measuring Magnetic fields

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Thurston,

I would insist that the EM supplier measures the room
fields and thus, if they accept the room, have
responsibility for achieving spec on the machine after
installation.

However, if you want to do a check - measure the X, Y and Z
components of the field in several places. In particular
the position where the most sensitive part of the
instrument will be. For a standard TEM this will be the
objective lens area, a FEGTEM will also need low fields at
the gun and if you have EELS then also at the spectrometer
position.

Also measure the fields in all 8 room corners to find if
there is a strong field gradient across the room, if there
is you probably have a local source that should be
investigated. If the field is high but without any gradient
across the room the source will probably be some distance
away.

Finally leave the gaussmeter in one position for some time
to check for any changes over time (eg. someone running
equipment intermittently). After all this you may still be
caught by the experiment that only runs for a few days
every so often and is never on long enough to locate it
(personal experience).

Good luck,
Ron


On Sun, 28 Apr 2002 15:02:41 -0700 (PDT) thurston e
herricks {thurst0n-at-u.washington.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello All,
}
} What are standard methods for measuring magnetic fields in a room?
} We need to know find out if a room meets specifications for installing a
} TEM. We have a gauss meter. However, we are unsure of a method to
} compare different rooms to one anouther for choosing the best location
} for the TEM.
}
} Thank you for your time
}
} Take care
}
} Thurston Herricks
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Mon Apr 29 03:50:12 2002



From: yimin yao :      yimin-at-fy.chalmers.se
Date: Mon, 29 Apr 2002 10:39:25 +0200
Subject: TEM iron ELNES in cementites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

I am working with catalytic nanoparticles in CNT. I need help to find the
ELNES for Fe in cementites (Fe3C, M7C3, M23C6 etc.), any information either
in literatures or obtained from experiments and computations.

Best regards,


Yiming


------------------------------------
Dr. Yiming Yao

Microscopy and Microanalysis
Experimental Physics
Chalmers University of Technology
SE-41296, Göteborg
Sweden

Tel: +46 31 7723633
Fax: +46 31 7723224
email: yimin-at-fy.chalmers.se
website: http://fy.chalmers.se/~yimin

-------------------------------------



From daemon Mon Apr 29 07:30:09 2002



From: joe.p.neilly-at-abbott.com
Date: Mon, 29 Apr 2002 07:19:19 -0500
Subject: Re: QX3 "toy" microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I know several teachers that would like to use this microscope on their
Macintosh computers. Has anyone found or written software that will run the
QX3 from a Mac?

Joe Neilly, Senior Microscopist
Abbott Laboratories
R45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027



Caroline
Schooley To: Microscopy-at-sparc5.microscopy.com
{schooley-at-mcn cc: gwc-at-u.arizona.edu, joe.p.neilly-at-abbott.com,
.org} mckernan-at-cems.umn.edu, nev-at-ccmr.cornell.edu, dillamanr-at-pop2.uncwil.edu,
pjohnson-at-iolok.com, mccann-at-tiac.net, dtaatjes-at-uvm.edu,
04/26/02 pfungosakR-at-aol.com, gwe-at-biotech.ufl.edu, sbarlow-at-sunstroke.sdsu.edu,
05:54 PM JeanDP-at-aol.com, judo888-at-yahoo.com
Subject: QX3 "toy" microscope





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Some of you have experimented with the Intel QX3 "toy" microscope, which is
now being marketed to teachers with more software (and a higher price). If
you're a real geek, a link to its CPiA command structre is given at
http://slashdot.org/science/02/04/21/1719259.shtml?tid=137 . The same URL
has an under-$50 ordering source. If you make the thing do new tricks,
please let us know!

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html








From daemon Mon Apr 29 08:05:21 2002



From: Richard Beanland :      richard.beanland-at-bookham.com (by way of
Date: Mon, 29 Apr 2002 07:56:06 -0500
Subject: RE: amorphous films of germanium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anne-Mette,
I believe this is actually very straightforward - just make a TEM
specimen from germanium using ion milling at reasonable kV (4-6). From my
rather hazy memory of this, I seem to remember the problem with Ge is
avoiding the amorphous film in thin specimens!

If you have no luck in finiding a commercial supplier I can knock one up for
you (I think I have some Ge lying around somewhere...)

Good luck,

Richard


_______________________________
Richard Beanland
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com





-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Anne-Mette Heie Kjaer wrote:
====================================================
} } Could someone help me finding where to buy a tem-grid with a film of
} amorphous
} } Germanium?
====================================================
I believe this would be difficult to make. I am not saying impossible, just
difficult. There would be some big-time safety concerns as well, so I have
been told.

But if your interest is mainly because you want something completely
structureless and featureless, yet something that is more robust than a
polymer film, what about the silicon nitride membrane window grids offered
by SPI Supplies, and described on URL
http://www.2spi.com/catalog/instruments/silicon-nitride.shtml

Compared to a polymer film, the nitride membrane is indestructable.

Chuck

Disclaimer: SPI Supplies offers a family of silicon nitride membrane window
grid products and therefore we would like to see more people using them!

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




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From daemon Mon Apr 29 08:15:38 2002



From: antmezzo-at-hotmail.com (by way of MicroscopyListserver)
Date: Mon, 29 Apr 2002 08:06:37 -0500
Subject: Ask-A-Microscopist:TEM Protocols Macromolecules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (antmezzo-at-hotmail.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
April 29, 2002 at 08:08:20
---------------------------------------------------------------------------

Email: antmezzo-at-hotmail.com
Name: Antonio Mezzogiorno

Organization: Institute of Human Anatomy

Education: Graduate College

Location: Naples, Italy

Question: Could You suggest me some protocols for TEM preparation of
macromolecules ?

Thanks in advance,

Antonio Mezzogiorno, MD, PhD

---------------------------------------------------------------------------


From daemon Mon Apr 29 09:08:09 2002



From: Augusto.A.Morrone-at-seagate.com
Date: Mon, 29 Apr 2002 08:59:50 -0500
Subject: Re: Method for measuring Magnetic fields

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Thurston:

Since magnetic fields is not the only issue you will face for your TEM
installation, I suggest you look at a comprehensive treatment in a paper by
D. Muller and J. Grazul (J. of Electron Microscopy, Vol 50, N0. 3, pp
219-226, 2001). They suggest methods of making a quick survey of EM
fields, as well as a number of fixes for the various sources of image
deterioration. A whole symposium was dedicated to this problem in the 2001
M&M conference. However, I encourage you to work with the TEM manufacturer
for the final room survey way ahead of installation since they may have the
last word in what is a compliant room environment for their instrument.

Augusto

Augusto A. Morrone, PhD
Seagate Technology RHO
NRW 115
7801 Computer Ave South
Minneapolis, MN 55435
(952) 402-5838
Fax: (952) 402-7301
Augusto.A.Morrone-at-seagate.com



thurston e
herricks To: Microscopy-at-sparc5.microscopy.com
{thurst0n-at-u.washi cc:
ngton.edu} Subject: Method for measuring Magnetic fields

04/28/2002 05:02
PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello All,

What are standard methods for measuring magnetic fields in a room?
We need to know find out if a room meets specifications for installing a
TEM. We have a gauss meter. However, we are unsure of a method to
compare different rooms to one anouther for choosing the best location
for the TEM.

Thank you for your time

Take care

Thurston Herricks







From daemon Mon Apr 29 09:47:05 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 29 Apr 2002 09:40:00 -0500
Subject: Etching TEM Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hans,

This is on the Microscopy net. I thought that you might want to answer.

Jim P.





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Frida,

How's Minnesota? Too bad about the snow last week. Typical MN Spring.
I'm a Minnesota Alumni and I still have family there. :)

There is a solution of sodium methoxide that should work on your cross
sections. It is made by making a saturated solution of sodium hydroxide
in methanol. Add 5 to 10 grams NaOH pellets to 50 ml methanol in a
dark glass bottle. Let it stand for two to three weeks before using it.
The longer it stands, the stronger it gets, so you may want to dilute it
more as it ages. Typically this solution is diluted in methanol 1
part solution to 3 parts methanol. Etching times differ according to
age of solution and thickness of the section. I can't find the original
reference for it, but S.R. Shi, et al used a version of it in the 90's
for
etching celloidin sections for immunohistochemistry. (Sorry, the web is
down here right now, or I'd give you the complete references.) Maybe
someone else knows the refs. and will respond.

The solution is a nasty one to have around, but it will work. I just put
a drop or two on the slide. Be careful not to over etch. Once etched,
I rinse the sections thoroughly with distilled water and continue with
the stain technique.

Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas, Dallas, Texas


"Frida.Maiers-at-co.hennepin.mn.us"-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are looking for a procedure to remove epoxy resin from polymerized
} blocks. The goal would be to eventually stain the tissue previously
} embedded for light microscopy and immunofluorescent techniques. Is this
} possible and would there be a reference that explains this procedure?
} Thanks,
} Frida Maiers
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Mon Apr 29 10:37:52 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 29 Apr 2002 10:29:54 -0500
Subject: Etching TEM Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hans,

This is on the Microscopy net. I thought that you might want to answer.

Jim P.





------------------------------------------------------------------------
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Hi Frida,

How's Minnesota? Too bad about the snow last week. Typical MN Spring.
I'm a Minnesota Alumni and I still have family there. :)

There is a solution of sodium methoxide that should work on your cross
sections. It is made by making a saturated solution of sodium hydroxide
in methanol. Add 5 to 10 grams NaOH pellets to 50 ml methanol in a
dark glass bottle. Let it stand for two to three weeks before using it.
The longer it stands, the stronger it gets, so you may want to dilute it
more as it ages. Typically this solution is diluted in methanol 1
part solution to 3 parts methanol. Etching times differ according to
age of solution and thickness of the section. I can't find the original
reference for it, but S.R. Shi, et al used a version of it in the 90's
for
etching celloidin sections for immunohistochemistry. (Sorry, the web is
down here right now, or I'd give you the complete references.) Maybe
someone else knows the refs. and will respond.

The solution is a nasty one to have around, but it will work. I just put
a drop or two on the slide. Be careful not to over etch. Once etched,
I rinse the sections thoroughly with distilled water and continue with
the stain technique.

Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas, Dallas, Texas


"Frida.Maiers-at-co.hennepin.mn.us"-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are looking for a procedure to remove epoxy resin from polymerized
} blocks. The goal would be to eventually stain the tissue previously
} embedded for light microscopy and immunofluorescent techniques. Is this
} possible and would there be a reference that explains this procedure?
} Thanks,
} Frida Maiers
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Mon Apr 29 11:19:42 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 29 Apr 2002 12:13:01 -0400
Subject: RE: Can I look at Hg?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Jonathan,

Vapor pressures of Hg are listed in AIP Handbook as follows:

Temp(Celcius) Pressure(atm, [760mmHg, 760 torr])
357 1
251 10-1
176 10-2
120 10-3
77 10-4
42 10-5
14 10-6

Seems to me that even at ESEM low vacs of ~2 torr the V.P. of Hg is going to
be between 120 and 176 degrees C. At higher vacs, lower temps, no solution.
Sounds like, unless amalgamated or otherwise contained, the Hg is going to
evaporate all over your system either from heat of beam or reduced vapor
pressure. Hope there is a better way!

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: jmkrupp-at-cats.ucsc.edu
} Sent: Friday, April 26, 2002 6:10 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: SEM: Can I look at Hg?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Can I look at metallic mercury in a conventional SEM?
}
} Here's the set up:
}
} A researcher makes electrodes of 'glassy carbon'. He adds a membrane of
} Nafion (teflon like, maybe 200 nm thick, makes like a holey formvar
} covering) to the surface.
}
} Then he plates metallic mercury through the pores of the membrane onto the
} electrode.
}
} He wants to know if the mercury fills up the pores and spills over onto
} the
} top of the membrane or not.
}
} We have a conventional SEM. I don't know if mercury will hold up in the
} chamber at normal SEM pressures. Anyone know for sure?
}
} If this won't work, any ideas for a Plan B?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}


From daemon Mon Apr 29 11:28:11 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 29 Apr 2002 12:21:46 -0400
Subject: SEM: Can I look at Hg?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you want to examine something with Hg in it where the Hg is not amalgamated, then you will have to cool the sample and keep it cooled. Hg has a vapor pressure of about 1E-03 Torr at room temperature. In your vacuum system of the microscope it will just go poof. Also note: Hg vapor is a poison and can get to you over time.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Friday, April 26, 2002 6:10 PM
To: Microscopy-at-sparc5.microscopy.com



Can I look at metallic mercury in a conventional SEM?

Here's the set up:

A researcher makes electrodes of 'glassy carbon'. He adds a membrane of
Nafion (teflon like, maybe 200 nm thick, makes like a holey formvar
covering) to the surface.

Then he plates metallic mercury through the pores of the membrane onto the
electrode.

He wants to know if the mercury fills up the pores and spills over onto the
top of the membrane or not.

We have a conventional SEM. I don't know if mercury will hold up in the
chamber at normal SEM pressures. Anyone know for sure?

If this won't work, any ideas for a Plan B?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Mon Apr 29 13:36:57 2002



From: Siebein, Kerry :      ksiebein-at-erc.ufl.edu
Date: Mon, 29 Apr 2002 14:13:34 -0400
Subject: TEM - Freeze Fracture Replication Technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am interested learning about the freeze fracture / replication technique
for preparation of micro emulsions for TEM. We have access to a Balzers 400
freeze fracture unit that has not been used in many years. The instrument
was moved here from another facility, and we are in the process of setting
it up for use.

Does any one have specific recommendations for this type of sample
preparation? I am interested in visiting a laboratory for training on
freeze fracture / replication technique. I have read numerous articles on
freeze fracture and we have the manual for the instrument, but I would
prefer to visit a laboratory that routinely freeze fractures samples for
hands on experience.

Thank you.

Kerry N. Siebein
University of Florida
Engineering Research Center for Particle Science and Technology
P. O. Box 116135
Gainesville, FL 32611-6135

(352) 846-1194
(352) 846-1196 fax
ksiebein-at-erc.ufl.edu



From daemon Tue Apr 30 07:23:44 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 30 Apr 2002 08:07:58 -0400
Subject: RE: Ask-A-Microscopist:TEM Protocols Macromolecules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Antonio,
I suggest a look at the offerings from Google when you search: "tem
macromolecules pdf".

Good luck,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: antmezzo-at-hotmail.com
} Sent: Monday, April 29, 2002 9:06 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist:TEM Protocols Macromolecules
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (antmezzo-at-hotmail.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} April 29, 2002 at 08:08:20
} --------------------------------------------------------------------------
} -
}
} Email: antmezzo-at-hotmail.com
} Name: Antonio Mezzogiorno
}
} Organization: Institute of Human Anatomy
}
} Education: Graduate College
}
} Location: Naples, Italy
}
} Question: Could You suggest me some protocols for TEM preparation of
} macromolecules ?
}
} Thanks in advance,
}
} Antonio Mezzogiorno, MD, PhD
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Tue Apr 30 09:08:46 2002



From: bmalon01-at-fiu.edu (by way of MicroscopyListserver)
Date: Tue, 30 Apr 2002 08:57:27 -0500
Subject: Ask-A-Microscopist: cathodluminescence & SEM

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (bmalon01-at-fiu.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
April 30, 2002 at 05:12:39
---------------------------------------------------------------------------

Email: bmalon01-at-fiu.edu
Name: Barb

Organization: FIU

Education: Graduate College

Location: Miami, Florida, USA

Question: I am convinced we should be able to use a
cathodluminescence detector on a SEM for
biological applications using fluorochromes or
immunogold labeling. It was stated to me that
certain facilities have attempted this exact same thing
I was told that the problem was quenching happened
to quickly to grab an image.
Any of you out there have any knowledge of this?
Thanks


---------------------------------------------------------------------------


From daemon Tue Apr 30 09:47:55 2002



From: Susan Rehorek :      susan.rehorek-at-sru.edu
Date: Tue, 30 Apr 2002 10:46:04 -0700
Subject: Camera Lucida

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a Camera Lucida. This is a drawing tube + dissecting
(stereo) microscope. I used these as an undergrad - and found them to be
excellent for zone diagrams and gross pictures (photos give too much detail
sometimes).

I have tried all the major vendors (in the US) I can think of.

Does anyone know where I can get these from? It doesn't have to be new, as
long as it works.

Please reply off-list.

Thanks

Sue
Susan J Rehorek, Ph.D.
Department of Biology
Slippery Rock University of Pennsylvania
PA, 16057-1326

ph: 724 738 2485
fax: 724 738 4782
email: susan.rehorek-at-sru.edu


From daemon Tue Apr 30 10:49:53 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Tue, 30 Apr 2002 17:41:44 +0200
Subject: Jeol 6700F SEM

Contents Retrieved from Microscopy Listserver Archives
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Hi all

I want to have a contact with other user of the Jeol 6700F FE-SEM, to
exchange informations. Are there other users from this SEM on the list ?
Please contact me off-line.

Thanks

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Tue Apr 30 10:54:05 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 30 Apr 2002 08:41:43 -0700
Subject: Re: TEM - Freeze Fracture Replication Technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I am interested learning about the freeze fracture / replication technique
} for preparation of micro emulsions for TEM. We have access to a Balzers 400
} freeze fracture unit that has not been used in many years. The instrument
} was moved here from another facility, and we are in the process of setting
} it up for use.
}
} Does any one have specific recommendations for this type of sample
} preparation? I am interested in visiting a laboratory for training on
} freeze fracture / replication technique. I have read numerous articles on
} freeze fracture and we have the manual for the instrument, but I would
} prefer to visit a laboratory that routinely freeze fractures samples for
} hands on experience.
}
} Thank you.
}
} Kerry N. Siebein
} University of Florida

Kerry -

You'll find the 2 special issues of J.E.M.Tech. [13(3-4):157-373(1989)
helpful. Lee & Owicki have a method on pgs 372-373 that may be what you
need; it avoids artefactual aggregation of liposomes by using propane jet
freezing.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Apr 30 11:38:15 2002



From: Margaret Miller :      MILLERMM-at-uthscsa.edu
Date: Tue, 30 Apr 2002 10:33:01 -0500
Subject: MT-2B service manual

Contents Retrieved from Microscopy Listserver Archives
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--
Dear list,
Does anyone have a service manual to the MT-2B Sorvall microtome.
We are in need of a parts list.
Thanks


From daemon Tue Apr 30 11:38:17 2002



From: Margaret Miller :      MILLERMM-at-uthscsa.edu
Date: Tue, 30 Apr 2002 10:33:01 -0500
Subject: MT-2B service manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--
Dear list,
Does anyone have a service manual to the MT-2B Sorvall microtome.
We are in need of a parts list.
Thanks


From daemon Tue Apr 30 12:53:39 2002



From: Margaret Miller :      MILLERMM-at-uthscsa.edu
Date: Tue, 30 Apr 2002 11:47:23 -0500
Subject: Service manual for MT-2B

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--
Dear list,
Does anyone have a service manual to the MT-2B Sorvall microtome.
We are in need of a parts list.
Thanks.


From daemon Tue Apr 30 13:27:29 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 30 Apr 2002 14:25:08 -0400
Subject: SEM: Can I look at Hg?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott & Folks.;

I found this material made by Acton Technologies that may be helpful in
working with mercury. The link is below.

http://www.actontech.com/hgx1.htm Below is cut from their site. I have no
interest, financial or otherwise with this vendor.

"For the reduction of toxic mercury vapor, HgX® is an inexpensive, water
soluble, non-hazardous, metallic-mercury/sulfide converting powder with a
chelating compound and dispersing agent. It reacts rapidly, forming a film
over the finely divided, nearly invisible beads of mercury and reacting to
produce a non-vaporizing sulfide. Further, HgX® keeps on working. Particles
of HgX® left on cleaned surfaces are reactivated during subsequent cleaning.


HgX® is not harmful. None of its components are classified as toxic. It
leaves no odor. And no allergic reactions have ever been reported even after
continuous use under various conditions.

HgX® is stable. It is supplied in 15 lb. polyethylene drums and can be
stored safely up to one year without any loss of activity. "

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Monday, April 29, 2002 12:22 PM
To: 'jmkrupp-at-cats.ucsc.edu'
Cc: Microscopy (E-mail)


If you want to examine something with Hg in it where the Hg is not
amalgamated, then you will have to cool the sample and keep it cooled. Hg
has a vapor pressure of about 1E-03 Torr at room temperature. In your
vacuum system of the microscope it will just go poof. Also note: Hg vapor
is a poison and can get to you over time.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Friday, April 26, 2002 6:10 PM
To: Microscopy-at-sparc5.microscopy.com



Can I look at metallic mercury in a conventional SEM?

Here's the set up:

A researcher makes electrodes of 'glassy carbon'. He adds a membrane of
Nafion (teflon like, maybe 200 nm thick, makes like a holey formvar
covering) to the surface.

Then he plates metallic mercury through the pores of the membrane onto the
electrode.

He wants to know if the mercury fills up the pores and spills over onto the
top of the membrane or not.

We have a conventional SEM. I don't know if mercury will hold up in the
chamber at normal SEM pressures. Anyone know for sure?

If this won't work, any ideas for a Plan B?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Tue Apr 30 14:57:03 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 30 Apr 2002 12:48:28 -0700
Subject: Horseman 120 roll film back

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There was a discussion thread here awhile ago
about cut film holders for use instead of Polaroid
materials. I have some 4x5" Riteway cut film holders for
sale and a Horseman 120 roll film back,
Mfr # 47022452. This roll film back fits Graflok backs
found on most all SEMs. It works with any type
of 120 roll film. The active image capture area
is 6x7cm--so you won't get the normal field of
view as that of a Polaroid or 4x5" cut film. But the
film and processing is much cheaper than Polaroid.

The holders are $6ea and the roll back is $425
($550 new). If no one is interested in these items,
they will go to a photo selling venue.

gary g.





From daemon Tue Apr 30 15:54:48 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Tue, 30 Apr 2002 15:48:07 -0500
Subject: Re: digital cameras again

Contents Retrieved from Microscopy Listserver Archives
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There are numerous differences between Coolpix
models and other units with the same resolution.
Construction, adaptation, color balance, luminance
control, and "real resolution" are just a few of them.
One of the biggest problems with consumer cameras
is the effect of anti-aliasing. The CP950 has a
published rez of 2.1M pixels while the CP990 and 995
are 3.3M pixels. But these are not real resolutions.
The final resolution suffers from anti-aliasing.
The 950, 990, 995 and 5000 use a USB remote cable release
puck which is very handy for microscope use.
Also, the AC adapter is quite useful. Rechargeable
NiMh AAs are very good for use in the 950 and 990.
But the AC adapter makes this a moot issue. The
5000 uses Li-Ion module.

The 900-series use a fixed lens whereas the 5000's
lens protrudes from the front when turned on.
The threaded lenses of the 900s are easy to
adapt. I have not seen a scope adapter for the
5000, but suspect that one would exist.

I hope the following is not redundant....
This is a nicely done discussion about Coolpixes
and anti-aliasing by user nghy taken from digital photo usenet:

} Hello,
}
} I have been working extensively with the Coolpix 990. In general the
} camera itself works well. As far as I know the two models differ in
} that the 995 uses the newer and thicker compact flash memory
} cards/mini disk memory and the flash unit pops up and away from the
} camera body to prevent red eyd in portraits. There may be other minor
} differences as well but for use at the microscope they are
} functionally the same. The Coolpix is able to close fcus without
} other lenes and is capable of some macro work with out additional
} optics which are also available.
}
} The main problems with consumer cameras are 1) they use an
} anti-aliasing routine to "soften" the image to prevent some odd pixel
} effects and 2). the CCD chips are not physically cooled to limit the
} thermal noise which in turn reduces the dynamic range of the images.
} The design of the camera is not easily altered to circumvent either
} of these issues. The best you can do is to use photo editing software
} to massage and sharpen the image.
}
} Paranthetically, here is some information on anti-aliasing filters.
} http://www.kodak.com/global/en/professional/products/cameras/dcsTech/antiAliasingFilter/antiAliasing.jhtml
}
} There is no clear-cut best coupler.
}
} High end photographic systems designed for use with a microscope use a
} specially designed projection lens (in place of an eyepiece) to cast
} the image on the film. Camera backs are used without other
} photographic lenses.
}
} The 990 and 995 do not have removable lenses which means an eyepiece
} of one sort or another is required to relay the image to the camera
} lens. ( In this circumstance, the camera lens is set to focus at
} infinity and the aperture is set at its widest. The image is focused
} with the microscope controls and should be parfocal with the image at
} the eyepieces. The only problem will be with your own eyesight. The
} camera will not focus at the same point as your eyes so you will need
} to use your glasses to focus unless you develope a standard correction
} to compensate for the difference.)
}
} Herein lies the rub. Each of the microscope manufacturers builds into
} their eyepieces compensation for residual uncorrected abberitions in
} their objectives. These corrections are unique for the manufacturer
} and for the human eye which is the normal primary detector.
}
} There are adapters that are designed to connect the Coolpix camera to
} whatever eyepiece is designed for the microscope. Theorectically this
} is the best solution but in practice these may or may not work
} depending on the eyepiece design. My Zeiss widefield high eyepoint
} eyepieces vignette severly when using this type of adapter. This type
} adapter has not provided adequate results for me.
}
} So I must use "another" eyepiece. I have used a Leitz Periplan
} eyepiece that happily screws onto the Coolpix and found that this
} pretty much works but not to perfection. I have also used another
} eyepiece adapter designed by Optem specificaly for use with the
} Coolpix. Here again the results are different from the Leitz
} eyepiece but the conclusion is the same, pretty good but not perfect.
}
} The point is that the digital images can be striking and quite
} appealing IF you have never seen the same image at the microscope.
} The best images I have ever captured are a 6 or 7 on a scale of
} 1(worst) to 10 (best) when compared to the vue at the scope. This is
} frustrating and I am still searching for an answer.
}
} Therefore, I have made a compromise because the costs of a camera that
} comes closer to perfection is going to cost between 3 and 8 times as
} much as the Coolpix..

If you need high quality images, you need a high quality
camera. Not a consumer one. But the cost difference
is significant. It really depends on how much quality
you need and/or can afford.

gary g.



At 02:09 PM 4/12/2002, you wrote:

} Is there any difference between the same resolution cameras?
}
} The most used looks to me is the Nikon Coolpix 950... isn't it? Anyone
} experimented for example with Kodak 4900?
}
} Keep care and be of good cheer
}
} Regards
}
} (name) Vratislav Richard Eugene Maria John Baptist
} (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
} (title) 84 duke of Siebenlügner
}
} websites:
} http://www.coleoptera.org. and
} http://www.egroups.com/group/coleoptera
}
} University of Sydney
} The Wentworth Bldg., B 62
} NSW 2006
} AUSTRALIA
} phone : +61 414 540 465
} email: vratislav-at-bigfoot.com
} ICQ: 13610107
}
} Only after the last tree has been cut down,
} only after the last river has been poisoned,
} only after the last fish has been caught,
} only then will you find that money can not be eaten.'
} CREE INDIAN PROPHECY.
}
} Incoming mail is certified Virus Free.
} Checked by AVG anti-virus system (http://www.grisoft.com).
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Tue Apr 30 19:53:10 2002



From: Rontec Cust. :      rtc998-at-toast.com
Date: 1 May 2002 00:43:15 -0000
Subject: RÖNTEC USA Closes Down Operation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America




Apologies to those who may consider this message inappropriate for the listserver, but the following information may be very important to other current or prospective users of EDX equipment sold by RÖNTEC USA, Inc.

RÖNTEC USA has been providing misleading information on its equipment, its commitment and ability to provide support, and its sales and financial situation. The company has recently made substantial cuts to its operations and may soon be forced to cease business completely. The company has vacated its Acton, Mass. laboratory and office space, eliminated its administrative and technical support staff, and is in the process of selling off its equipment (e.g., see www.mwrn.com/page/rontec/leo440.asp). At the moment, RÖNTEC USA retains a nominal existence by operating out of a private home, but its future remains uncertain. The company has apparently exhausted its startup capital without demonstrating self-sustainability and was effectively cut off from cash by its parent, RÖNTEC GmbH.

Anyone considering the purchase of systems or components from RÖNTEC USA is advised to seek further information directly from the company.

__________________________________________
Join http://www.toast.com today for your own free, flash-based webmail!



From daemon Tue Apr 30 21:08:27 2002



From: zaluzec-at-microscopy.com
Date: Tue, 30 Apr 2002 20:57:20 -0500
Subject: Administrivia: Anonymous Posting are against our Rules!

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References: {20020501004315.36422.qmail-at-toast.com}


Colleagues....

It is inappropriate to post information to the listserver without
providing your true identity. This is one of the first rules of this
listserver and was instituted at the onset to insure that honesty
and integrity is maintained. To this end anonymous posting of messages,
was always disallowed. This is even more disturbing when it
involves unsubstantiated information which has
direct implications upon either individuals and/or companies.


The most recent posting concerning Rontec USA is an example
of this. I have no information about the companies status, but because of the
anonymous posting I will personnally contact Rontec USA and inform
them of the information which was posted.

Email address which are not easily identified with an individual
are obviously common, however, if you post a message I expect
you to add your real name to the posting!

The Email address of the individual who posted this anonymous
message will hereby be banned from posting to the listserver,
until (s)he properly identifies themself.


Nestor
Your Friendly (but not Pleased) Neighborhood SysOp






From daemon Wed May 1 08:33:21 2002



From: msteglic-at-mail.mdanderson.org
Date: Wed, 1 May 2002 08:17:07 -0500
Subject: Camera Lucida

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Did you try Nikon? I have one that was was purchased from them about 10 years
ago. The part number on it is 231262.
---------------------- Forwarded by Mannie Steglich/MDACC on 05/01/2002 08:15 AM
---------------------------


Susan Rehorek {susan.rehorek-at-sru.edu} on 04/30/2002 12:46:04 PM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Mannie Steglich/MDACC)


I am looking for a Camera Lucida. This is a drawing tube + dissecting
(stereo) microscope. I used these as an undergrad - and found them to be
excellent for zone diagrams and gross pictures (photos give too much detail
sometimes).

I have tried all the major vendors (in the US) I can think of.

Does anyone know where I can get these from? It doesn't have to be new, as
long as it works.

Please reply off-list.

Thanks

Sue
Susan J Rehorek, Ph.D.
Department of Biology
Slippery Rock University of Pennsylvania
PA, 16057-1326

ph: 724 738 2485
fax: 724 738 4782
email: susan.rehorek-at-sru.edu






From daemon Wed May 1 08:33:25 2002



From: msteglic-at-mail.mdanderson.org
Date: Wed, 1 May 2002 08:22:18 -0500
Subject: MT-2B service manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I have one. If you will contact me directly by email, we can make arrangements
for what you need.

Mannie Steglich
U T M D Anderson Cancer Center.
---------------------- Forwarded by Mannie Steglich/MDACC on 05/01/2002 08:22 AM
---------------------------


Margaret Miller {MILLERMM-at-uthscsa.edu} on 04/30/2002 10:33:01 AM

To: MSA: ;
cc: (bcc: Mannie Steglich/MDACC)



--
Dear list,
Does anyone have a service manual to the MT-2B Sorvall microtome.
We are in need of a parts list.
Thanks






From daemon Wed May 1 08:46:30 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 1 May 2002 09:44:52 -0400
Subject: Horseman 120 roll film back

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;

Does anyone know of a mfg. that makes a camera, for general photography,
that will accomodate 4x5" film like Polaroid Type 57, that uses a Graflok
back? I checked with Polaroid but they could offer no assistance.

Thanks,

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Tuesday, April 30, 2002 3:48 PM
To: MSA listserver


There was a discussion thread here awhile ago
about cut film holders for use instead of Polaroid
materials. I have some 4x5" Riteway cut film holders for
sale and a Horseman 120 roll film back,
Mfr # 47022452. This roll film back fits Graflok backs
found on most all SEMs. It works with any type
of 120 roll film. The active image capture area
is 6x7cm--so you won't get the normal field of
view as that of a Polaroid or 4x5" cut film. But the
film and processing is much cheaper than Polaroid.

The holders are $6ea and the roll back is $425
($550 new). If no one is interested in these items,
they will go to a photo selling venue.

gary g.





From daemon Wed May 1 09:52:10 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 01 May 2002 11:23:31 -0400
Subject: Philips CM12 for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It makes one wonder why anyone would post something on the Listserver &
conceal their identity
unless they had something to hide.

Then again, why would someone post an message like this unless they had
something to gain or in revenge.

I havE neVer heard of rontec but assume that thEy are a X-ray company.
For motivation, that lEaVes compEting X-ray Companies.
HowEVer, I doubt that the largEr X-ray Companies would even bother.
That leaves the smaller EDS Companies which would have equal motivation but
would not see the repercussions of management.

I seem to vaguely recall a thread about a member evaluating EDS Companies &
their service.
Another member touted the superior service & capabilities of one of the EDS
Companies in question.
A colleague of mine questioned the identity of the second member & found
that the second member had posted the message anonymously.
When queried about their identity he got no response.

It would be interesting to identify this person.

I am not saying that there is a connection, just thinking out loud on the
Listserver.

Earl Weltmer
Scanservice Corporation
My true identity
----- Original Message -----
} From: {zaluzec-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Cc: "Rontec Cust." {rtc998-at-toast.com}
Sent: Tuesday, April 30, 2002 6:57 PM


I have a Philips CM12 TEM for sale. It was under service contract from
Philips until about 3 years ago. It has not been used for over a year
due to a vacuum leak in the column. All manuals and lots of spare parts
are included, including the gaskets needed to repair the leak. Water
recirculator is also included. The microscope is in central New Jersey.
Best offer.
Please contact me directly.

Geoff
mcauliff-at-umdnj.edu
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed May 1 11:41:29 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 1 May 2002 11:33:02 -0500
Subject: Horseman 120 roll film back

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter,

Most, if not all, makes of 4x5 camera will accept Polaroid backs. I bought one years ago from Calumet Photo for about $200 (without lens!. The camera was a used Cambo 4x5, built like a tank.

Nikon makes a 35mm camera that will accept Polaroid film with the use of an expensive accessory, called a "magny", I believe. You could check with your local camera store on this, but a decent used 4x5 will certainly cost less.

Hope this helps.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Wednesday, May 01, 2002 8:45 AM
To: MSA listserver


Folks;

Does anyone know of a mfg. that makes a camera, for general photography,
that will accomodate 4x5" film like Polaroid Type 57, that uses a Graflok
back? I checked with Polaroid but they could offer no assistance.

Thanks,

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Tuesday, April 30, 2002 3:48 PM
To: MSA listserver


There was a discussion thread here awhile ago
about cut film holders for use instead of Polaroid
materials. I have some 4x5" Riteway cut film holders for
sale and a Horseman 120 roll film back,
Mfr # 47022452. This roll film back fits Graflok backs
found on most all SEMs. It works with any type
of 120 roll film. The active image capture area
is 6x7cm--so you won't get the normal field of
view as that of a Polaroid or 4x5" cut film. But the
film and processing is much cheaper than Polaroid.

The holders are $6ea and the roll back is $425
($550 new). If no one is interested in these items,
they will go to a photo selling venue.

gary g.






From daemon Wed May 1 11:58:46 2002



From: Raj Patel :      rpatel-at-umdnj.edu
Date: Wed, 01 May 2002 12:56:30 -0400
Subject: Cryo pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




We have a Balzer 500 freeze fracture unit that needs a new cryo pump.

Since Balzer no longer support its equipments we are having a difficult time
finding parts and people to service the unit.

Any used balzer cryo pump available out there? Any other palces?

Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
675 Hoes Lane
Piscataway, NJ 08854




From daemon Wed May 1 15:54:27 2002



From: Mary K. O'Connell :      oconne1l-at-leland.stanford.edu
Date: Wed, 01 May 2002 13:45:53 -0700
Subject: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Need Your Help!!

I am trying to teach 4th graders how an oxygen saturation meter works, & my
experiment is FAILING!! Basically, oxygen saturation is measured by
passing 2 light beams through the finger, one red light (660 nm), the other
infrared (940 nm). If the blood is oxygenated, it appears red because it
absorbs red light, thus very little of the red beam passes through. If the
blood has little oxygen, it appears blue & allows red light to pass. In
both cases, the infrared beam will pass through. The amount of red vs
infrared light detected on the other side of the finger is proportional to
level of oxygen saturation.

I designed an experiment to show that a red laser pointer beam would be
absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
the laser beam darker if anything! PLEASE, can someone tell me what is
going on? Is there any simple way to communicate this principle?? I
appreciate your help.

Mary

***************************************************
Mary K.O'Connell
Cardiovascular Biomechanics Research Lab
MSLS: Room P224 - Surgery
1201 Welch Road
Stanford, CA 94305
(650) 723-1695
(650) 498-6262 Fax
E-mail: oconne1l-at-leland.stanford.edu
(Note: O'Connell in the E-mail address is spelled with a numeric "1" for
the first l, and an alphabetic "l" for the second.)
*******************************************************


From daemon Wed May 1 16:29:31 2002



From: Phoebe J Doss/app/Cvm :      pjdoss-at-cvm.okstate.edu
Date: Wed, 1 May 2002 16:22:59 -0500
Subject: sectioning "hot" tissue

Contents Retrieved from Microscopy Listserver Archives
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"Ginger
Hendricks" To: "Doss, Phoebe and Mike" {pjdoss-at-okstate.edu}
{grhendricks-at-cox. cc: (bcc: Phoebe J Doss/app/Cvm)
net} Subject: please forward to the MSA listserv

05/01/02 04:13 PM






Hello all,

I have a researcher who is interested in using autoradiography with nerve
tissue. His tech is researching methods and has come to me for advice. I
have never performed this procedure and am concerned about sectioning "hot"
(S35) tissue with the microtome. Any protocols, suggestions or direction
would be greatly appreciated.

My email address is grhendricks-at-cox.net

Thank you in advance,

Ginger

Ginger R. Hendricks
EM Lab Manager and Adjunct Instructor
OSU-CHS
1111 W. 17th St.
Tulsa, OK 74107
(918) 561-8232 work
(918) 699-8629 fax
grhendricks-at-cox.net
Website:
http://www.healthsciences.okstate.edu/research/rsp/electronmicroscopy/electron_microscopy_laboratory.htm






From daemon Wed May 1 18:47:46 2002



From: Earl Weltmer :      earlw-at-sbcglobal.net (by way of MicroscopyListserver)
Date: Wed, 1 May 2002 18:36:45 -0500
Subject: copper wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

This might be a little off-topic but does anyone know where I can purchase
some copper wire for use in an FESEM?

The wire would probably need to be "oxygen-free" and withstand some elevated
temperature.

Thank You,

Earl Weltmer
(Real identity except to ex-wife & unknown offspring)




From daemon Wed May 1 19:53:06 2002



From: John Brealey :      john.brealey-at-imvs.sa.gov.au
Date: Thu, 2 May 2002 10:15:30 +0930
Subject: Darkroom printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there,
Does anyone have a ballpark figure for the cost of a new tabletop automatic
processor for black and white printing? Currently we use an Ilford 2150RC
processor.
We are thinking of switching to digital printing off negatives and are doing
some costings.
Thanks.

John Brealey,
EM Unit,
Queen Elizabeth Hospital,
Adelaide,
South Australia



From daemon Wed May 1 23:43:22 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 01 May 2002 21:34:43 -0700
Subject: Re: sectioning "hot" tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As soon as it's in the plastic, it should not be a problem. S35 - is very
moderate beta emitter, 26 cm of air/0.32 mm of water will completely block
it. Half-life is 84 days - keep in mind: work faster than decay. Most
important part is labelling and embedding. Check solutions for
radionuclides after processing the sample with Geiger detector, dispose
properly. Amount of radioactivity in sections is negligible: less or equal
to uranyl-acetate staining.
Hope it may help. Sergey

At 02:22 PM 5/1/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu May 2 00:03:32 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 2 May 2002 10:45:14 +0000
Subject: Re: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Raj, was the cryopump made by Balzers? If not, I suspect it was made by CTI
Cryogenics - try www.ctivacuum.com for parts, service, or replacement. I
don't know for sure if they have used/rebuilt - they may. Also try
www.bidservice.com for used cryopumps and Helium compressors.

No interest in either one, just passing the information along.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Raj Patel {rpatel-at-umdnj.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 01, 2002 12:56 PM


Earl, try these:

1) Malin Co., 5400 Smith Road, Brook Park, OH 44142. Specialty wire. Tel.
(216)267-9080. I don't know if they have a web site.
2) Alfa Aesar, www.alfa.com , (800)343-0660, and (800)343-7276 for tech
support.
3) Little Falls Alloys, www.lfa-wire.com , (888)5329473 or (973)278-1666.

Alfa will probably have the purest Copper, as far as Oxygen and other
impurities go (the best is 99,9999% metals basis). Little Falls Alloys may
have more suitable specialty Copper alloy, especially for high temperature
apps.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Earl Weltmer (by way of MicroscopyListserver) {earlw-at-sbcglobal.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 01, 2002 7:36 PM


Mary
Isn't this backwards? Oxygenated blood appears redder in
transmitted or reflected light because it absorbs *blue*, and
transmits or reflects the remaining red.
} Basically, oxygen saturation is measured by
} passing 2 light beams through the finger, one red light (660 nm), the other
} infrared (940 nm). If the blood is oxygenated, it appears red because it
} absorbs red light, thus very little of the red beam passes through. If the
} blood has little oxygen, it appears blue & allows red light to pass. In
} both cases, the infrared beam will pass through. The amount of red vs
} infrared light detected on the other side of the finger is proportional to
} level of oxygen saturation.

Again I think you have this the wrong way round. Red Kool-Aid
looks red because it allows red light to pass through. Think of the
effect of shining a white torch beam through red KoolAid onto a
sheet of white paper. What colour is the beam? Red. Why,
because that is the predominant colour of light remaining in the
beam, blue and green having been removed by the KoolAid. A Blue
filter should attenuate a red laser more than a Red filter, assuming
the two are of the same colour strength.

The only way the red KoolAid can absorb more red light than the
blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
is present in the Blue KoolAid. This seems unlikely, but is not
impossible. Which flavour do you use for red, and which for blue?
The crimson effect of black cherry is almost certainly produced by
combining red and blue dyes, and there may be more blue dye in
black cherry than there is in ice-blue raspberry.

You might find the following web sites instructive

http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
y.html#dyestruct
http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
um.html
Chris

} I designed an experiment to show that a red laser pointer beam would be
} absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
} the laser beam darker if anything! PLEASE, can someone tell me what is
} going on? Is there any simple way to communicate this principle?? I
} appreciate your help.
}
} Mary
}
} ***************************************************
} Mary K.O'Connell
} Cardiovascular Biomechanics Research Lab
} MSLS: Room P224 - Surgery
} 1201 Welch Road
} Stanford, CA 94305
} (650) 723-1695
} (650) 498-6262 Fax
} E-mail: oconne1l-at-leland.stanford.edu
} (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} the first l, and an alphabetic "l" for the second.)
} *******************************************************
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Thu May 2 09:13:29 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 02 May 2002 08:58:53 -0500
Subject: Re: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mary,

I think Chris is right. I got your message late last night and started
to reply that plants are green because they reflect green light, then I
in my sleepy state I started thinking that maybe something is different
about reflected versus transmitted light and I gave up trying to help.

However, this morning I still think the red should pass through red
Koolaid better than ultraviolet. I haven't had this stuff since my
undergrad about 20 years ago.

Karen Pawlowski, Ph.D.
UT Dallas

Chris Jeffree wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Mary
} Isn't this backwards? Oxygenated blood appears redder in
} transmitted or reflected light because it absorbs *blue*, and
} transmits or reflects the remaining red.
} } Basically, oxygen saturation is measured by
} } passing 2 light beams through the finger, one red light (660 nm), the other
} } infrared (940 nm). If the blood is oxygenated, it appears red because it
} } absorbs red light, thus very little of the red beam passes through. If the
} } blood has little oxygen, it appears blue & allows red light to pass. In
} } both cases, the infrared beam will pass through. The amount of red vs
} } infrared light detected on the other side of the finger is proportional to
} } level of oxygen saturation.
}
} Again I think you have this the wrong way round. Red Kool-Aid
} looks red because it allows red light to pass through. Think of the
} effect of shining a white torch beam through red KoolAid onto a
} sheet of white paper. What colour is the beam? Red. Why,
} because that is the predominant colour of light remaining in the
} beam, blue and green having been removed by the KoolAid. A Blue
} filter should attenuate a red laser more than a Red filter, assuming
} the two are of the same colour strength.
}
} The only way the red KoolAid can absorb more red light than the
} blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
} is present in the Blue KoolAid. This seems unlikely, but is not
} impossible. Which flavour do you use for red, and which for blue?
} The crimson effect of black cherry is almost certainly produced by
} combining red and blue dyes, and there may be more blue dye in
} black cherry than there is in ice-blue raspberry.
}
} You might find the following web sites instructive
}
} http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
} y.html#dyestruct
} http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
} um.html
} Chris
}
} } I designed an experiment to show that a red laser pointer beam would be
} } absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} } pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
} } the laser beam darker if anything! PLEASE, can someone tell me what is
} } going on? Is there any simple way to communicate this principle?? I
} } appreciate your help.
} }
} } Mary
} }
} } ***************************************************
} } Mary K.O'Connell
} } Cardiovascular Biomechanics Research Lab
} } MSLS: Room P224 - Surgery
} } 1201 Welch Road
} } Stanford, CA 94305
} } (650) 723-1695
} } (650) 498-6262 Fax
} } E-mail: oconne1l-at-leland.stanford.edu
} } (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} } the first l, and an alphabetic "l" for the second.)
} } *******************************************************
} }
}
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================



From daemon Thu May 2 12:57:33 2002



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Thu, 02 May 2002 10:48:13 -0700
Subject: sectioning "hot" tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ginger,

we do microtomy/TEM of bacterial samples with U and Tc in our lab. We take
special precautions at the block trimming part by collecting all the chips
on a piece of tape and disposing of them, as well as of the unused sections,
as a rad waste. I don't think my little routine of wiping the microtome
after finishing the work helps anything but my consciousness, however, it
may make you feel better, too. Since I am using a TEM that is NOT devoted to
the rad work (I think that will be also your case), I make a duplicate grid,
and run it through the scintillation counter. If the count doesn't exceed
background (most of the cases - imagine the dimensions of a section), I
consider it non rad. Your S35 beta should be a very safe bet.
Please feel free to contact me if you need any more info,
Alice.

Alice Dohnalkova
Environmental Microbiology
Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692 office
(509) 376-3654 TEM lab
fax (509) 376-1321

} From: Phoebe J Doss/app/Cvm [SMTP:pjdoss-at-cvm.okstate.edu]
Sent: Wednesday, May 01, 2002 2:23 PM
To: microscopy-at-sparc5.microscopy.com




"Ginger

Hendricks" To: "Doss, Phoebe and
Mike" {pjdoss-at-okstate.edu}
{grhendricks-at-cox. cc: (bcc: Phoebe J
Doss/app/Cvm)
net} Subject: please forward to
the MSA listserv


05/01/02 04:13 PM









Hello all,

I have a researcher who is interested in using autoradiography with nerve
tissue. His tech is researching methods and has come to me for advice. I
have never performed this procedure and am concerned about sectioning "hot"
(S35) tissue with the microtome. Any protocols, suggestions or direction
would be greatly appreciated.

My email address is grhendricks-at-cox.net

Thank you in advance,

Ginger

Ginger R. Hendricks
EM Lab Manager and Adjunct Instructor
OSU-CHS
1111 W. 17th St.
Tulsa, OK 74107
(918) 561-8232 work
(918) 699-8629 fax
grhendricks-at-cox.net
Website:
http://www.healthsciences.okstate.edu/research/rsp/electronmicroscopy/electr
on_microscopy_laboratory.htm






From daemon Thu May 2 13:08:26 2002



From: Schmitz, Robert :      rschmitz-at-uwsp.edu
Date: Thu, 2 May 2002 13:02:31 -0500
Subject: RE: SEM and TEM for bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suggest that you check out the following reference

Dickson, G.R., 1984; Chemical fixation and the preparation of calcified tissues for transmission electron microscopy
in: G.R. Dickson (ed) Methods of Calcified Tissue Research. New York, Elsevier, pp 79-148

Bob
Robert J. Schmitz
Department of Biology
University of Wisconsin Stevens Point
Stevens Point, WI 54481
715-346-2420
Email: rschmitz-at-uwsp.edu
http://biology.uwsp.edu/faculty/RSchmitz/home.html

} ----------
} From: Fernando Capela e Silva
} Sent: Wednesday, April 24, 2002 5:20 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: SEM and TEM for bone
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would like someone send me a general bone preparation scheme for SEM and
} TEM.
}
} With compliments
} * * * * * * * * * * * * * * * * * * * * * *
} Fernando Capela e Silva
} Laboratório de Biologia da Conservação
} Departamento de Biologia
} Universidade de Évora
} Apartado 94
} 7002-554 Évora
} PORTUGAL
}
} Phone: +351-266 760 800
} Fax: +351-266 711 231
} Email: fcs-at-uevora.pt
}
} http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm
}
}
}


From daemon Thu May 2 13:37:17 2002



From: Karli Fitzelle :      fitzelle.1-at-osu.edu (by way of MicroscopyListserver)
Date: Thu, 2 May 2002 14:56:13 -0500
Subject: Grainy negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you Michael. It is really nice of you!!!

Xianglin

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411


----- Original Message -----
} From: Michael Green {michael.green-at-UCL.AC.UK}


Hello all!!
I have a quick question for everyone.....We have a Hitachi
7500 TEM. We have been having a problem with "grainy" negatives, I
don't think the focus is as crisp as it should be. I have tried
smaller apertures (both obj. and cond. in various combinations). If
we take pictures of the same sample on a different microscope, the
micrographs are not grainy and the focus is much clearer (so it's not
the sample, the negatives, or the paper we're using....). I know the
optical density setting for the camera can be changed to increase
contrast, I have a feeling this would increase graininess as
well...is my hunch correct? The kicker is that we have had the same
optical density setting for weeks now and the graininess is just
getting worse (to my knowledge nothing has changed on the
microscope....). Would the use (or lack of use) of liquid Nitrogen
influence graininess? Any other ideas or comments?
Any help would be appreciated!!

Thanks,

Karli


From daemon Thu May 2 16:26:51 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 2 May 2002 17:43:22 -0400
Subject: Re: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Check out a Beldon Cable catalog. They sell different gauges of a good grade of Copper cable (unshielded/unwrapped). The copper conductor in the cables is probably a good grade also, but it may not be OFHC (Oxygen-free High Conductivity). I have no idea whether Beldon has their catalog on the web or not.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Vitaly Feingold [mailto:vitalylazar-at-worldnet.att.net]
Sent: Thursday, May 02, 2002 2:47 AM
To: Microscopy-at-sparc5.microscopy.com; Earl Weltmer (by way of
MicroscopyListserver)


Earl, try these:

1) Malin Co., 5400 Smith Road, Brook Park, OH 44142. Specialty wire. Tel.
(216)267-9080. I don't know if they have a web site.
2) Alfa Aesar, www.alfa.com , (800)343-0660, and (800)343-7276 for tech
support.
3) Little Falls Alloys, www.lfa-wire.com , (888)5329473 or (973)278-1666.

Alfa will probably have the purest Copper, as far as Oxygen and other
impurities go (the best is 99,9999% metals basis). Little Falls Alloys may
have more suitable specialty Copper alloy, especially for high temperature
apps.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Earl Weltmer (by way of MicroscopyListserver) {earlw-at-sbcglobal.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 01, 2002 7:36 PM


Heaven forbid that I should comment on something in the life science, so I won't. I don't think that Chris is correct in his explanation.

Let me site the example of a thin film of gold. If it is thin enough, when you look at the gold in transmission, it is green. In reflection it is gold because the gold is absorbing the green and allowing all other wavelengths of light to be reflected. If you make the gold thicker the intensity of the green getting through decreases exponentially with thickness until eventually it is opaque. A series of thickness of gold films from very thin to very thick illustrates the point very well.

I suspect that the absorption mechanism with the oxygen is related to the iron in the blood. Isn't it iron in the blood that carries the oxygen? With more oxygenated iron, a higher absorption occurs, i.e. the red light gets absorbed more. This is analogous to your beam getting darker. I would say that to show a less oxygenated example, you have to dilute your Cool-Aid.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
Sent: Thursday, May 02, 2002 6:45 AM
To: Mary K. O'Connell
Cc: microscopy-at-sparc5.microscopy.com


Mary
Isn't this backwards? Oxygenated blood appears redder in
transmitted or reflected light because it absorbs *blue*, and
transmits or reflects the remaining red.
} Basically, oxygen saturation is measured by
} passing 2 light beams through the finger, one red light (660 nm), the other
} infrared (940 nm). If the blood is oxygenated, it appears red because it
} absorbs red light, thus very little of the red beam passes through. If the
} blood has little oxygen, it appears blue & allows red light to pass. In
} both cases, the infrared beam will pass through. The amount of red vs
} infrared light detected on the other side of the finger is proportional to
} level of oxygen saturation.

Again I think you have this the wrong way round. Red Kool-Aid
looks red because it allows red light to pass through. Think of the
effect of shining a white torch beam through red KoolAid onto a
sheet of white paper. What colour is the beam? Red. Why,
because that is the predominant colour of light remaining in the
beam, blue and green having been removed by the KoolAid. A Blue
filter should attenuate a red laser more than a Red filter, assuming
the two are of the same colour strength.

The only way the red KoolAid can absorb more red light than the
blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
is present in the Blue KoolAid. This seems unlikely, but is not
impossible. Which flavour do you use for red, and which for blue?
The crimson effect of black cherry is almost certainly produced by
combining red and blue dyes, and there may be more blue dye in
black cherry than there is in ice-blue raspberry.

You might find the following web sites instructive

http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
y.html#dyestruct
http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
um.html
Chris

} I designed an experiment to show that a red laser pointer beam would be
} absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
} the laser beam darker if anything! PLEASE, can someone tell me what is
} going on? Is there any simple way to communicate this principle?? I
} appreciate your help.
}
} Mary
}
} ***************************************************
} Mary K.O'Connell
} Cardiovascular Biomechanics Research Lab
} MSLS: Room P224 - Surgery
} 1201 Welch Road
} Stanford, CA 94305
} (650) 723-1695
} (650) 498-6262 Fax
} E-mail: oconne1l-at-leland.stanford.edu
} (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} the first l, and an alphabetic "l" for the second.)
} *******************************************************
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Thu May 2 17:12:47 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 2 May 2002 23:09:07 +0100
Subject: Re: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Yeeess!, but haemoglobin is not reflective like gold. The gold
phenomenon is caused by strong forward reflection of red/yellow light,
and transmission of remaining green. Blood cells have no surface
reflectivity to speak of. Blood is more like an emulsion of a red
transparent phase in a pale yellow transparent phase. The oxygenated
rbcs absorb blue, transmitting red, but they also cause light
scattering, and some red light is forward scattered as a result.
Unlike a film of gold, blood is red in reflected and transmitted
light.

Now how do you explain the fact that colloidal gold appears red
against the light i.e. red is transmitted??
Chris

p.s. you're allowed - we're talking physics here
} Heaven forbid that I should comment on something in the life
science, so I won't. I don't think that Chris is correct in his
explanation.
}
} Let me site the example of a thin film of gold. If it is thin
enough, when you look at the gold in transmission, it is green. In
reflection it is gold because the gold is absorbing the green and
allowing all other wavelengths of light to be reflected. If you make
the gold thicker the intensity of the green getting through decreases
exponentially with thickness until eventually it is opaque. A series
of thickness of gold films from very thin to very thick illustrates
the point very well.
}
} I suspect that the absorption mechanism with the oxygen is related
to the iron in the blood. Isn't it iron in the blood that carries the
oxygen? With more oxygenated iron, a higher absorption occurs, i.e.
the red light gets absorbed more. This is analogous to your beam
getting darker. I would say that to show a less oxygenated example,
you have to dilute your Cool-Aid.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} P. O. Box 11472 (letters)
} Guys Run Rd. (packages)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8515 (fax)
}
}
}
} -----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
} Sent: Thursday, May 02, 2002 6:45 AM
} To: Mary K. O'Connell
} Cc: microscopy-at-sparc5.microscopy.com
} Subject: Re: Basic Science - oxygen saturation example
}
}
} --------------------------------------------------------------------
----
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
---.
}
}
} Mary
} Isn't this backwards? Oxygenated blood appears redder in
} transmitted or reflected light because it absorbs *blue*, and
} transmits or reflects the remaining red.
} } Basically, oxygen saturation is measured by
} } passing 2 light beams through the finger, one red light (660 nm),
the other
} } infrared (940 nm). If the blood is oxygenated, it appears red
because it
} } absorbs red light, thus very little of the red beam passes
through. If the
} } blood has little oxygen, it appears blue & allows red light to
pass. In
} } both cases, the infrared beam will pass through. The amount of red
vs
} } infrared light detected on the other side of the finger is
proportional to
} } level of oxygen saturation.
}
} Again I think you have this the wrong way round. Red Kool-Aid
} looks red because it allows red light to pass through. Think of the
} effect of shining a white torch beam through red KoolAid onto a
} sheet of white paper. What colour is the beam? Red. Why,
} because that is the predominant colour of light remaining in the
} beam, blue and green having been removed by the KoolAid. A Blue
} filter should attenuate a red laser more than a Red filter, assuming
} the two are of the same colour strength.
}
} The only way the red KoolAid can absorb more red light than the
} blue is if it contains more red-absorbing (i.e. cyan or blue) dye
than
} is present in the Blue KoolAid. This seems unlikely, but is not
} impossible. Which flavour do you use for red, and which for blue?
} The crimson effect of black cherry is almost certainly produced by
} combining red and blue dyes, and there may be more blue dye in
} black cherry than there is in ice-blue raspberry.
}
} You might find the following web sites instructive
}
} http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
} y.html#dyestruct
} http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
} um.html
} Chris
}
} } I designed an experiment to show that a red laser pointer beam
would be
} } absorbed by red Kool-Aid, while blue Kool-Aid would allow the red
beam to
} } pass through. Unfortunately, this is not the case!!! The red
Kool-Aid makes
} } the laser beam darker if anything! PLEASE, can someone tell me
what is
} } going on? Is there any simple way to communicate this principle??
I
} } appreciate your help.
} }
} } Mary
} }
} } ***************************************************
} } Mary K.O'Connell
} } Cardiovascular Biomechanics Research Lab
} } MSLS: Room P224 - Surgery
} } 1201 Welch Road
} } Stanford, CA 94305
} } (650) 723-1695
} } (650) 498-6262 Fax
} } E-mail: oconne1l-at-leland.stanford.edu
} } (Note: O'Connell in the E-mail address is spelled with a numeric
"1" for
} } the first l, and an alphabetic "l" for the second.)
} } *******************************************************
} }
}
}
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================



From daemon Thu May 2 17:27:37 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 2 May 2002 16:26:39 -0600
Subject: Re: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't want to make this more complicated as it is, but do you know, how
Koolaid gets its color?

Could be straight exitation and relaxation. Then Koolaid would absorb a
photon and emit it again. Since the emission should be into 4 Pi solid
angle, the effect would be, that fewer photons of that color reach the eye,
i.e., this color would be absorbed.

Could be a "fluorescence" effect, where Koolaid absorbs a high energy photon
(blue), then emits a red photon and an IR photon. In this case the red parts
of the spectrum would be enhanced.

Could be scattering, as in "why is the sky blue".

And then, the laser frequency may not be the one that is actually absorbed
by Koolaid. Red is not necesarily red.

I think, without knowing exactly what it is that makes Koolaid red it is
hard to predict what happens. And if that then can be applied to the color
of blood is another step.

It might be easier to start with something different than Koolaid.



} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Karen Pawlowski [mailto:kpawlow-at-swbell.net]
Sent: Thursday, May 02, 2002 7:59 AM
To: MSA listserver submission


Mary,

I think Chris is right. I got your message late last night and started
to reply that plants are green because they reflect green light, then I
in my sleepy state I started thinking that maybe something is different
about reflected versus transmitted light and I gave up trying to help.

However, this morning I still think the red should pass through red
Koolaid better than ultraviolet. I haven't had this stuff since my
undergrad about 20 years ago.

Karen Pawlowski, Ph.D.
UT Dallas

Chris Jeffree wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Mary
} Isn't this backwards? Oxygenated blood appears redder in
} transmitted or reflected light because it absorbs *blue*, and
} transmits or reflects the remaining red.
} } Basically, oxygen saturation is measured by
} } passing 2 light beams through the finger, one red light (660 nm), the
other
} } infrared (940 nm). If the blood is oxygenated, it appears red because it
} } absorbs red light, thus very little of the red beam passes through. If
the
} } blood has little oxygen, it appears blue & allows red light to pass. In
} } both cases, the infrared beam will pass through. The amount of red vs
} } infrared light detected on the other side of the finger is proportional
to
} } level of oxygen saturation.
}
} Again I think you have this the wrong way round. Red Kool-Aid
} looks red because it allows red light to pass through. Think of the
} effect of shining a white torch beam through red KoolAid onto a
} sheet of white paper. What colour is the beam? Red. Why,
} because that is the predominant colour of light remaining in the
} beam, blue and green having been removed by the KoolAid. A Blue
} filter should attenuate a red laser more than a Red filter, assuming
} the two are of the same colour strength.
}
} The only way the red KoolAid can absorb more red light than the
} blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
} is present in the Blue KoolAid. This seems unlikely, but is not
} impossible. Which flavour do you use for red, and which for blue?
} The crimson effect of black cherry is almost certainly produced by
} combining red and blue dyes, and there may be more blue dye in
} black cherry than there is in ice-blue raspberry.
}
} You might find the following web sites instructive
}
} http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
} y.html#dyestruct
} http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
} um.html
} Chris
}
} } I designed an experiment to show that a red laser pointer beam would be
} } absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam
to
} } pass through. Unfortunately, this is not the case!!! The red Kool-Aid
makes
} } the laser beam darker if anything! PLEASE, can someone tell me what is
} } going on? Is there any simple way to communicate this principle?? I
} } appreciate your help.
} }
} } Mary
} }
} } ***************************************************
} } Mary K.O'Connell
} } Cardiovascular Biomechanics Research Lab
} } MSLS: Room P224 - Surgery
} } 1201 Welch Road
} } Stanford, CA 94305
} } (650) 723-1695
} } (650) 498-6262 Fax
} } E-mail: oconne1l-at-leland.stanford.edu
} } (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} } the first l, and an alphabetic "l" for the second.)
} } *******************************************************
} }
}
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================



From daemon Thu May 2 17:57:59 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 02 May 2002 15:50:53 -0700
Subject: Re: Grainy negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karli

If it's focus problem, try to make 'focus series' - the same area with
1,2,3 clicks to the left and to the right. You have to see the changes on
the negs (and on the screen). I usually align microscope in the way that I
am able to see changes from underfocus to overfocus in 3 clicks (it's
JEM1200EX, coarse control) at x60-80K. So, 1st click is underfocus, next-
focus, next - overfocus. If you do not see it, you have, probably, problem
with alignment. I don't know how this related to your particular
problem. In biological applications we are usually using amplitude
contrast, so it's close to the actual focus. At this point you have
minimal visual contrast. From that you defocus (to increase contrast and
'granularity' will go up too) your image. The degree of defocus depends
what you want to see.

Personally, I would suspect film/developer/technician. So many things: bad
batch of the film, film stored at the high temp, contaminated developer,
unproper dilution etc. Try to make blank image (no sample in the
microscope), turn off automatic exposure and make a few shots with
different exposure time (around automatic measurements, say automatic is 2
sec, make 1, 1.5, 2, 3 sec) and develop altogether....

Sergey

At 12:56 PM 5/2/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu May 2 19:12:56 2002



From: pcy :      pcy-at-usc.edu
Date: Thu, 2 May 2002 17:05:27 -0700 (PDT)
Subject: confocal--overlapping signal problem?

Contents Retrieved from Microscopy Listserver Archives
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We have stained annelid embryos with FITC-conjugated anti-tubulin antibody
and propidium iodide. We are using a Biorad-MRC-600 and seem to be getting
almost identical signals(of nuclei and a few mitochondria) on both the 488
and 568 wavelengths, even though when viewed with epifluorescence, the
tubulin stain seems to distinctly define cell borders. I understood that
the propidium iodide emission would not be overlapping with that of
FITC--is this incorrect? I have other double-labelled samples which are
stained with different stains, but their signals are within the
appropriate emission spectra for both confocal and epifluorescence, so the
detection filters on the confocal do not seem to be the problem.

Any feedback is appreciated!

Pauline Yu
pcy-at-usc.edu
Manahan Lab
http://www.usc.edu/manahanlab



From daemon Thu May 2 19:54:02 2002



From: pjp6 :      pjp6-at-dana.ucc.nau.edu
Date: Thu, 02 May 2002 17:47:16 -0700
Subject: More digital questions: MDS 100

Contents Retrieved from Microscopy Listserver Archives
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I was considering purchasing a Kodak MDS 100 Digital camera to permanently
attach to one of my microscopes for examining specimens directly on my
computer CRT. Unfortunately as far as I can determine the software supplied
only supports Win 95 and 98. I'd like to know if anyone is currently using a
MDS 100 on ME or XP and how (or if) it works.
Thanks in advance
P Polsgrove

Pete Polsgrove
NAU Flagstaff, AZ.
pjp6-at-dana.ucc.nau.edu
micro2001p-at-netscape.net



From daemon Thu May 2 20:37:21 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Thu, 2 May 2002 21:37:59 -0500
Subject: Re: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
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Karli, do grainy negatives look denser? If so, they may be overexposed (TEM
setting/exp.meter problem), or overdeveloped
(developer/temperature/time/stop bath). I assume here that the batch of film
is fine. If grainy negatives don't look denser, then the problem is likely
with TEM. It's difficult to say whether you are dealing with real photo
emulsion grain, or rather with sample/TEM artifact, without seeing the
negative.

Speaking of the emulsion grain- it will also appear on the normal density
negatives, in the following cases: film improperly stored or expired,
negatives were overexposed and underdeveloped or vice versa, in order to
keep density normal. Then, bad developer (contaminated/exhausted), or bad
film.

Use Sergey's advice and take a few blanks. It's hard to imagine a TEM
problem causing that, other than incorrect exposure meter reading.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Karli Fitzelle (by way of MicroscopyListserver) {fitzelle.1-at-osu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 02, 2002 3:56 PM


Mary,
As someone who has used a pulse oximeter before a double lung transplant, I
decided to dig out my manual. I am copying the following FYI:

"The pulse oximeter shines red and infrared light through the tissue and
detects the fluctuating signals caused by arterial blood pulses. The ratio
of the fluctuations of the red and infrared light signals received
determines the oxygen saturation content. Conditions such as steady venous
blood flow, skin thickness, finger nail thickness, etc. do not effect the
saturation reading because they are constant and do not cause fluctuations."

(the function is then given as a formula; it goes on saying)

"Note that the oximeter's readings do not depend upon the absolute light
intensity, rather upon the fluctuations in light intensity."

It goes on with other comments about skin pigmentation, if there is too
little light, etc.

"Pulse oximeters use two different wavelengths of light (red and infrared),
providing the ability to determine one component of the blood. The oximeter
is calibrated to closely approximate functional oxygen saturation values.
These values will closely approximate laboratory instrument functional
saturation values if the dysfunctional hemoglobin saturation levels are
negligible. If the dysfunctional hemoglobin is carboxyhemoglobin or
methemoglobin, the difference between the oxygen saturation value displayed
by the oximeter and the oxygen saturation values determined by the
laboratory instrument will be greater as the dysfunctional hemoglobin levels
rise approximately in accordance with the following formula: " (it then
gives the formula).

I wonder if the issue is not "red blood" but how the red and infrared light
interact with oxygenated and deoxygenated hemoglobin and not what we see as
arterial (red) blood or venous (blue) blood. I don't think that this is an
easy principle for 4th graders.

Chris gives a correct explanation about light absorption and the dyes used
in Kool-Aid.

PS I hope this is part of a "Don't smoke" program!!!

Damian

Damian Neuberger, PhD
Senior Research Scientist
Baxter Healthcare Corp
P.O. Box 490
Round Lake, IL 60073
Tel: 847.270.5888
Fax: 847.270.5897
damian_neuberger-at-baxter.com







} Need Your Help!!
}
} I am trying to teach 4th graders how an oxygen saturation meter works, &
my
} experiment is FAILING!! Basically, oxygen saturation is measured by
} passing 2 light beams through the finger, one red light (660 nm), the
other
} infrared (940 nm). If the blood is oxygenated, it appears red because it
} absorbs red light, thus very little of the red beam passes through. If the
} blood has little oxygen, it appears blue & allows red light to pass. In
} both cases, the infrared beam will pass through. The amount of red vs
} infrared light detected on the other side of the finger is proportional to
} level of oxygen saturation.
}
} I designed an experiment to show that a red laser pointer beam would be
} absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} pass through. Unfortunately, this is not the case!!! The red Kool-Aid
makes
} the laser beam darker if anything! PLEASE, can someone tell me what is
} going on? Is there any simple way to communicate this principle?? I
} appreciate your help.
}
} Mary
}
} ***************************************************
} Mary K.O'Connell
} Cardiovascular Biomechanics Research Lab
} MSLS: Room P224 - Surgery
} 1201 Welch Road
} Stanford, CA 94305
} (650) 723-1695
} (650) 498-6262 Fax
} E-mail: oconne1l-at-leland.stanford.edu
} (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} the first l, and an alphabetic "l" for the second.)
} *******************************************************
}
}




From daemon Thu May 2 21:54:49 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Thu, 2 May 2002 21:49:23 -0500
Subject: Re: 4X5 Graflok Cameras

Contents Retrieved from Microscopy Listserver Archives
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Peter,

Check with Calumet Photographic. Their web address is

www.calumetphoto.com

Damian Neuberger

} Folks;
}
} Does anyone know of a mfg. that makes a camera, for general photography,
} that will accomodate 4x5" film like Polaroid Type 57, that uses a Graflok
} back? I checked with Polaroid but they could offer no assistance.
}
} Thanks,
}
} Peter Tomic
} Anadigics, Inc.





From daemon Thu May 2 23:50:31 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 02 May 2002 21:41:59 -0700
Subject: RE: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
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Scott and Chris

Talking about ultra-fine gold foil or gold colloidal suspension, we have to
keep in mind the'wave-nature' of the light. When thickness of material is
comparable to wavelength, so may magical things is happening: suspended
tiny gold particles colored solution in the red. Moreover, the color
depends from the particle size - you could make it brownish or greenish -
depends how much tannic acid you add to make collodial gold. Over
moreover: you could calculate the particles size from the known
spectrum. Similar things is happening with fine gold foil. I was a bad
student in the physic class. I do remember it's waves. If particle is
smaller than 1/2 wavelength, the wave will not interfere with it. Red
light has longer wavelength and pass gold particles when other has
adsorbed. I hope it's how they teach us... Sorry if I am wrong. Similar
to lasers. It's monochromatic and coherent. To filter it you should use
appropriate things: diffraction (pattern?) filters (they work similarly:
let pass wavelength longer then the size of the strips). If KoolAid is
looks red it does not mean it will be transparent to the laser
wavelength. I think the set of simple photographic filters (red/blue;
yellow/green) may do better job (I mean, no lasers).


Sergey


At 02:43 PM 5/2/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri May 3 01:15:42 2002



From: Bruyntjes, J.P. :      J.Bruyntjes-at-voeding.tno.nl
Date: Fri, 3 May 2002 08:07:05 +0200
Subject: unsubcribe

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From daemon Fri May 3 03:25:09 2002



From: =?ISO-8859-1?Q?Thomas_Sch=FClein?= :      Schuelein-at-roentec.de
Date: Fri, 3 May 2002 10:15:34 +0100
Subject: RONTEC USA Inc., Correction

Contents Retrieved from Microscopy Listserver Archives
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{bold} {color} {param} 0100,0100,0100 {/param} To the Microscopy & Microanalysi=
s community


{/bold} Ref.: Anonymous Message posted April 30,

Subj: R=D6NTEC USA Closes Down Operation


Ladies and Gentlemen:


Without any doubt, the Microscopy ListServer is a great facility for
the Microscopy&Microanalysis community to distribute and
exchange useful information in a very efficient way and everyone
involved in initiation and maintenance of this forum highly deserves
our community=92s credit.


However, it appears that at the same time this platform is a quite
powerful tool for anyone with hostile and bad enough intents to
defame and defile disliked entities. This is what we had to learn
when the unwarranted message regarding an alleged close down of
R=D6NTEC USA, Inc. was spread. It also appears that there is no
way to avoid such unfounded, false and misleading information to
be posted at the ListServer other than the commitment of all users
to the =93General Ground Rules on using the Microscopy
Listserver/Mailreflector=94 (see msa website), clearly a code of honor.
Whatever individual or organization posted this message in their
malicious attempt of damaging our company and good reputation
violated all those general ground rules. May everyone draw his/her
own conclusions concerning the nature of the information and the
integrity of the sender.


We may be allowed to correct the above-mentioned statements
about R=D6NTEC USA, Inc. as follows:


{paraindent} {param} out {/param} 1. There are no plans whatsoever to cease th=
e business of
R=D6NTEC USA, Inc. {/paraindent}


{paraindent} {param} out {/param} 2. The R=D6NTEC Group of companies =96 like =
so many others =96
has not remained unaffected by the overall downturn we
saw in the economy during the year 2001. The situation
within our group forced us to implement a restructuring
program in order to reduce operational costs (as was the
case throughout the industry =96 we believe we are in good
company here). This program was comprising all members
of our group, including RONTEC USA. The goal was not to
cease business, but exactly the opposite - to ensure its
continuation, in the best interest of our customers. {/paraindent}


{paraindent} {param} out {/param} 3. Our joint efforts have been successful. T=
he fact is
R=D6NTEC USA, Inc. has had its best sales year since the
company=92s founding in 1999 nearly doubling its revenue
over the previous year. Why, after having worked very hard
indeed to get things going and after having eventually
accomplished that stage, should we consider to
discontinue the business? Apparently there exists
someone or some company who is attempting to damage
our reputation. Be assured that R=D6NTEC USA is in a
better position than ever before and will continue to grow
and continue to support its customers. {/paraindent}


{paraindent} {param} out {/param} 4. We have never provided misleading informa=
tion on the
equipment we are offering. How long would we survive in
this business if we were to make promises we can=92t keep?
Due to our companies policy of verifying any information
before disclosing it to the public we earned ourselves a
reputation as a reliable business partner. {/paraindent}


{paraindent} {param} out {/param} 5. R=D6NTEC USA, Inc. does have an SEM for s=
ale, however
this has absolutely no bearing on the viability of the
company. {/paraindent}


Whoever has any questions or the need for more information on
this subject may please contact us directly, by phone or otherwise,
in the US or at our German headquarters. We at R=D6NTEC take
great pride in our products and services and we have nothing to
hide =96 in contrast to the sender of that unfounded message, who
chose not to disclose a name.

In the future may our community be spared such unwarranted
assaults.



Thomas Schuelein Paul Smith

President & CEO President & CEO

R=D6NTEC Holding AG R=D6NTEC USA, Inc.


{nofill}


From daemon Fri May 3 05:59:28 2002



From: barbara maloney :      bmalon01-at-fiu.edu
Date: Fri, 03 May 2002 06:48:41 -0400
Subject: confocal laser fluorescent

Contents Retrieved from Microscopy Listserver Archives
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Hi folks - I'm familar with prep for TEM, SEM, etc., but not confocal -
would you send me a good protocol for this type of scope for tissue or
single cells?
Thanks
Barb



From daemon Fri May 3 05:59:28 2002



From: barbara maloney :      bmalon01-at-fiu.edu
Date: Fri, 03 May 2002 06:51:21 -0400
Subject: confocal laser fluorescent

Contents Retrieved from Microscopy Listserver Archives
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Hi folks - I'm familar with prep for TEM, SEM, flow, etc., but not this
type of scope, would someone send me a good protocol for tissue and
single cells?
Thanks
Barb



From daemon Fri May 3 06:01:54 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 3 May 2002 11:55:31 +0000
Subject: Re: Basic Science - oxygen saturation example

Contents Retrieved from Microscopy Listserver Archives
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Yes, they do reflect green light. But they also transmit green light.
Chlorophylls a and b have two strong absorption peaks very roughly
between 400&500nm and between 650 and 700nm. The remaining
central part of the visible spectrum appears green, but also
contains cyan, yellow and orange.

} I think Chris is right. I got your message late last night and
started
} to reply that plants are green because they reflect green light, then I
} in my sleepy state I started thinking that maybe something is different
} about reflected versus transmitted light and I gave up trying to help.
}
} However, this morning I still think the red should pass through red
} Koolaid better than ultraviolet. I haven't had this stuff since my
} undergrad about 20 years ago.
}
} Karen Pawlowski, Ph.D.
} UT Dallas
}
} Chris Jeffree wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Mary
} } Isn't this backwards? Oxygenated blood appears redder in
} } transmitted or reflected light because it absorbs *blue*, and
} } transmits or reflects the remaining red.
} } } Basically, oxygen saturation is measured by
} } } passing 2 light beams through the finger, one red light (660 nm), the other
} } } infrared (940 nm). If the blood is oxygenated, it appears red because it
} } } absorbs red light, thus very little of the red beam passes through. If the
} } } blood has little oxygen, it appears blue & allows red light to pass. In
} } } both cases, the infrared beam will pass through. The amount of red vs
} } } infrared light detected on the other side of the finger is proportional to
} } } level of oxygen saturation.
} }
} } Again I think you have this the wrong way round. Red Kool-Aid
} } looks red because it allows red light to pass through. Think of the
} } effect of shining a white torch beam through red KoolAid onto a
} } sheet of white paper. What colour is the beam? Red. Why,
} } because that is the predominant colour of light remaining in the
} } beam, blue and green having been removed by the KoolAid. A Blue
} } filter should attenuate a red laser more than a Red filter, assuming
} } the two are of the same colour strength.
} }
} } The only way the red KoolAid can absorb more red light than the
} } blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
} } is present in the Blue KoolAid. This seems unlikely, but is not
} } impossible. Which flavour do you use for red, and which for blue?
} } The crimson effect of black cherry is almost certainly produced by
} } combining red and blue dyes, and there may be more blue dye in
} } black cherry than there is in ice-blue raspberry.
} }
} } You might find the following web sites instructive
} }
} } http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
} } y.html#dyestruct
} } http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
} } um.html
} } Chris
} }
} } } I designed an experiment to show that a red laser pointer beam would be
} } } absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} } } pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
} } } the laser beam darker if anything! PLEASE, can someone tell me what is
} } } going on? Is there any simple way to communicate this principle?? I
} } } appreciate your help.
} } }
} } } Mary
} } }
} } } ***************************************************
} } } Mary K.O'Connell
} } } Cardiovascular Biomechanics Research Lab
} } } MSLS: Room P224 - Surgery
} } } 1201 Welch Road
} } } Stanford, CA 94305
} } } (650) 723-1695
} } } (650) 498-6262 Fax
} } } E-mail: oconne1l-at-leland.stanford.edu
} } } (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} } } the first l, and an alphabetic "l" for the second.)
} } } *******************************************************
} } }
} }
} } ==========================================
} } Dr. Chris Jeffree
} } University of Edinburgh
} } BIOSEM - Biological Sciences Electron Microscope Facility
} } Institute of Cell and Molecular Biology
} } Waddington Building
} } King's Buildings, Mayfield Road
} } EDINBURGH, EH9 3JN, Scotland, UK
} } Tel. #44 (0) 131 650 5554
} } FAX. #44 (0) 131 650 6563
} } Mobile 07710 585 401
} } email c.jeffree-at-ed.ac.uk
} } =========================================
}
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Fri May 3 07:12:57 2002



From: Gordon Couger :      gcouger-at-couger.com (by way of MicroscopyListserver)
Date: Fri, 3 May 2002 07:02:01 -0500
Subject: Re: Grainy negatives

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} From a processing point of view:

Every time I have had a really excessive grain problem it was from using too
strong a concentration of developer, way too strong or I was underexposing
the film and over developing it. The first was usualy by accident and only
caused a problem when I was working with a completely unknown set of
conditions such as infra red photography, a new film and a mistake in
dilution where I had to establish exposure time, development time without
reliable tools to measure the intensity of the infra red light or working
with some new combination of film and some unconventional developer. I the
last case it was usualy intentionally trying to take pictures in light to
low for the film I had.


Gordon

} From: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
: Karli
:
: If it's focus problem, try to make 'focus series' - the same area with
: 1,2,3 clicks to the left and to the right. You have to see the changes on
: the negs (and on the screen). I usually align microscope in the way that I
: am able to see changes from underfocus to overfocus in 3 clicks (it's
: JEM1200EX, coarse control) at x60-80K. So, 1st click is underfocus, next-
: focus, next - overfocus. If you do not see it, you have, probably,
problem
: with alignment. I don't know how this related to your particular
: problem. In biological applications we are usually using amplitude
: contrast, so it's close to the actual focus. At this point you have
: minimal visual contrast. From that you defocus (to increase contrast and
: 'granularity' will go up too) your image. The degree of defocus depends
: what you want to see.
:
: Personally, I would suspect film/developer/technician. So many things:
bad
: batch of the film, film stored at the high temp, contaminated developer,
: unproper dilution etc. Try to make blank image (no sample in the
: microscope), turn off automatic exposure and make a few shots with
: different exposure time (around automatic measurements, say automatic is 2
: sec, make 1, 1.5, 2, 3 sec) and develop altogether....
:
: Sergey
:
: At 12:56 PM 5/2/02, you wrote:
: } ------------------------------------------------------------------------
: } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
: } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
: } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
: } -----------------------------------------------------------------------.
: }
: }
: } Hello all!!
: } I have a quick question for everyone.....We have a Hitachi 7500
: } TEM. We have been having a problem with "grainy" negatives, I don't
think
: } the focus is as crisp as it should be. I have tried smaller apertures
: } (both obj. and cond. in various combinations). If we take pictures of
the
: } same sample on a different microscope, the micrographs are not grainy
and
: } the focus is much clearer (so it's not the sample, the negatives, or the
: } paper we're using....). I know the optical density setting for the
camera
: } can be changed to increase contrast, I have a feeling this would
increase
: } graininess as well...is my hunch correct? The kicker is that we have had
: } the same optical density setting for weeks now and the graininess is
just
: } getting worse (to my knowledge nothing has changed on the
: } microscope....). Would the use (or lack of use) of liquid Nitrogen
: } influence graininess? Any other ideas or comments?
: } Any help would be appreciated!!
: }
: } Thanks,
: }
: } Karli
:
: _____________________________________
:
: Sergey Ryazantsev Ph. D.
: Electron Microscopy
: UCLA School of Medicine
: Department of Biological Chemistry
: Box 951737
: Los Angeles, CA 90095-1737
:
: Phone: (310) 825-1144
: FAX (departmental): (310) 206-5272
: mailto:sryazant-at-ucla.edu
:
:
:
:
:


From daemon Fri May 3 07:46:49 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 03 May 2002 05:39:46 -0700
Subject: Re: RONTEC USA Inc., Correction

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It's strange to see that one anonymous message could make so much damage to
the company. It seems to me that reputation of the company depends more on
the quality of the products/service etc nor on the one anonymous
opinion. From another hand, I think, this ListServer mostly is a place for
'customers' to exchange not only scientific ideas but to help each other to
survive in this 'capitalistic' world. Information about wrongdoing may
help others do not make similar mistake or spend money on bad quality
product/service. This is sort of our 'protection' against bad quality
service/products providers. Again, single decision even published here
could not damage the reputation of the good company with long well
established record of the service to EM community. I know, there are
places on the Internet where you could leave your opinion about some
particular business/company - it's perfectly legal and many institutions
used that lists for decision making. This discussion is more about ethics
- we all agree that we have rights openly speak here and others have the
rights to know who is speaking.

Sergey spoke.

At 02:15 AM 5/3/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri May 3 09:40:31 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 3 May 2002 10:34:07 -0400
Subject: RE: Basic Science - oxygen saturation example

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Morning All,

To get a better sense of this thread of conversation I went looking for a
source of concentration on this specific subject. This continued an attempt
some time ago when the question came up on another 'List'.

As we learn from one another, sometimes it helps to find a source of
expertise who appears to have actually been involved in the engineering of
the type of device in question - or something like it. Further, the
following URL demonstrates that this conversation is ALL about physics

Thus, I recommend some outside reading at:
http://lfd.uiuc.edu/staff/maier/thesis/index.html

Is it widely known that some arctic fish travel contentedly sans RBCs? For
more information look in old 'Comparative Biochemistry' by Baldwin. Short
book from Methuen and a longer one from Cambridge. Also a reading from Sci.
Am.: http://oak.cats.ohiou.edu/~piccard/scientam/globins.html

Also, HIGHLY recommended is the translation of Zinoffsky's original paper on
hemoglobin: http://www.udel.edu/chem/white/teaching/CHEM342/ZinBkgd99.html

For me life is just a constant stream of history and future, mixed in the
present. As George Patton said (about something else!), "God help me, I do
love it so."

Regards,

Fred Monson

P.S. Award for best Commencement Address ever goes to Kurt Vonnegut (1997 -at-
MIT) even if he didn't give it:
http://www.tiac.net/users/sqltech/document/vonegut.txt

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Mary K. O'Connell
} Sent: Wednesday, May 1, 2002 4:45 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Basic Science - oxygen saturation example
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Need Your Help!!
}
} I am trying to teach 4th graders how an oxygen saturation meter works, &
} my
} experiment is FAILING!! Basically, oxygen saturation is measured by
} passing 2 light beams through the finger, one red light (660 nm), the
} other
} infrared (940 nm). If the blood is oxygenated, it appears red because it
} absorbs red light, thus very little of the red beam passes through. If the
}
} blood has little oxygen, it appears blue & allows red light to pass. In
} both cases, the infrared beam will pass through. The amount of red vs
} infrared light detected on the other side of the finger is proportional to
}
} level of oxygen saturation.
}
} I designed an experiment to show that a red laser pointer beam would be
} absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} pass through. Unfortunately, this is not the case!!! The red Kool-Aid
} makes
} the laser beam darker if anything! PLEASE, can someone tell me what is
} going on? Is there any simple way to communicate this principle?? I
} appreciate your help.
}
} Mary
}
} ***************************************************
} Mary K.O'Connell
} Cardiovascular Biomechanics Research Lab
} MSLS: Room P224 - Surgery
} 1201 Welch Road
} Stanford, CA 94305
} (650) 723-1695
} (650) 498-6262 Fax
} E-mail: oconne1l-at-leland.stanford.edu
} (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} the first l, and an alphabetic "l" for the second.)
} *******************************************************
}
}


From daemon Fri May 3 09:40:32 2002



From: Peter Roiz :      peterroi-at-eml.doe.gov
Date: Fri, 03 May 2002 10:28:06 -0400
Subject: Oxygen examples.

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Try food coloring.



From daemon Fri May 3 09:41:21 2002



From: John Foust :      jfoust-at-threedee.com
Date: Fri, 03 May 2002 09:33:38 -0500
Subject: Re: Basic Science - oxygen saturation example

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At 09:37 PM 5/2/2002 -0500, Damian Neuberger wrote:
} I wonder if the issue is not "red blood" but how the red and infrared light
} interact with oxygenated and deoxygenated hemoglobin and not what we see as
} arterial (red) blood or venous (blue) blood. I don't think that this is an
} easy principle for 4th graders.

Yes, half of them will come away thinking that red Kool-Aid has
blood in it. :-) The good news is that 20% of them weren't
paying attention and won't retain this "fact".

- John



From daemon Fri May 3 11:28:15 2002



From: Ann-Fook Yang :      yanga-at-EM.AGR.CA
Date: Fri, 03 May 2002 11:26:25 -0400
Subject: Flat embedding of LR White

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Hi everyone,

I need to do flat embedding using LR White. A Teflon mold covered with ACLAR seems to be the answer. I am afraid that the over filled resin under ACLAR may not polimerize properly and become messy. I appreciate your experience.

Ann Fook Yang



From daemon Fri May 3 12:00:48 2002



From: Mike Coviello :      coviello-at-mae.uta.edu
Date: Fri, 03 May 2002 12:01:11 -0700
Subject: EM-A question for AN10000 owners

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Hi All:
I am trying to convert an image and spectra from the Oxford AN 10000 EDS
to
a .tiff that is readable on a PC. I've done it in the past, but it has
been so long, I forget the exact
procedure. I hope that there is somone out there that does this more
regularly that can refresh my memory.
Thanks in advance,
Mike Coviello
UT Arlington



From daemon Fri May 3 12:08:13 2002



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Fri, 03 May 2002 13:03:41 -0400
Subject: JEOL 4000 TEM available

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Dear Microscopists,

The following TEM, which is capable of producing images with 0.17
nm resolution, but has gun/filament instability problems especially above
300 KV, is available to anyone willing to take responsibility for removing
it from its present location prior to August 2002. There is no charge
other than the costs involved with dismantling, moving, shipping and
reinstallation. It is a top-entry version of a JEOL 4000. The only
accessory is a Gatan image intensifier and TV camera. It is estimated that
it would take two weeks to dismantle and pack properly for reuse. It is
located in Ithaca, NY at Cornell University's Center for Materials Research.


John Hunt
CCMR Microscopy Facility
(607) 255-0108



From daemon Fri May 3 13:07:45 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 3 May 2002 19:02:28 +0100
Subject: Re: Oxygen saturation example

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} Try food coloring.
Tried it. Didn't like it. Fact is green potatoes and bright purple
steak
just doesn't get eaten.

The take-home from this discussion, as pointed out by several
respondents, is that there are several ways to arrive at "red" in
Kool-Aid, inkjet inks, food colouring, whatever. For example, a red
which exactly matches the apparent colour of a laser pointer could be
produced by a single red dye, or, as in an inkjet printer, from a
blend of yellow and magenta dyes. A deeper cherry red from your
printer will be a blend of yellow, magenta and cyan dyes. What is
required is some data on the absorption/transmission characteristics
of different reds and blues. The best thing would be to run an
absorption spectrum using a spectrophotometer. If there is no chance
of access to one then it might be instructive to do some simple
chromatography. Place drops of concentrated solutions of fibre-tip
pen, fabric dye, food colouring or Kool-Aid one inch from the long
edge of a ~12" x 6" piece of filter paper or blotting paper. Curl
the paper into a cylinder, holding the short edges together with a
staple or paper clip and stand (the paper!) in 3/8" of water in a
suitable lidded container. 15-20 minutes later you will have a
colourful chromatographic separation of the main dyes in your samples.
Get the kids involved - they'll love it.

Chris




From daemon Fri May 3 13:59:21 2002



From: Parvez Haris :      pharis-at-dmu.ac.uk
Date: Fri, 3 May 2002 19:52:32 +0100
Subject: First International Conference on Biomedical Spectroscopy: From M

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Invitation and Call for Papers.

The First International Conference on Biomedical Spectroscopy:
} From Molecules to Men
7-10 July, 2002
Cardiff, Wales, United Kingdom

This conference covers spectroscopic as well as application of microscopic
techniques, such as AFM, for analysis of biological/biomedical systems.

Information regarding the conference is given below. You can also visit the
web-site of the conference:

http://www.dmu.ac.uk/In/biospectra/

Best wishes,
Dr P.I. Haris
Editor-in-chief: Spectroscopy - An International Journal
Editor: Biochemical Journal
Department of Biological Sciences,
De Montfort University, The Gateway, Leicester,
LE1 9BH, United Kingdom
E-Mail: pharis-at-dmu.ac.uk

First International Conference on Biomedical Spectroscopy:
} From Molecules to Men
7-10 July, 2002
Cardiff, Wales, United Kingdom

Aims and Scope of the Conference:

The first international conference on Biomedical Spectroscopy will bring
together spectroscopists and life scientists, from academia and
industry, engaged in solving problems of biological and biomedical
interest using diverse spectroscopic techniques. The conference is
dedicated to the memory of Professor Dennis Chapman FRS who introduced
many spectroscopic techniques to the study of biological systems.
The interdisciplinary nature of the conference will encourage scientific
interchange and cross-fertilisation of ideas.

Application of spectroscopic techniques such as
Mass spectrometry, CD, FTIR, NMR, EPR, ENDOR, NIR, SPR, Raman, EXAFS,
UV/Visible, Atomic Force Spectroscopy, Neutron Spectroscopy, Dielectric
Spectroscopy, Fluorescence Spectroscopy and so on,
for solving problems in diverse areas of life science including
-Biomedical and Clinical Science
-Biochemistry and Biophysics
-Biomaterials and Biosensors
-Proteomics and Pharmaceuticals
-Biotechnology and Biomedical Engineering

Call for Papers:

Scientists employing spectroscopic techniques in any area of life
science research are invited to submit proposals for lectures and poster
presentations. Papers presented at the conference will be reviewed and
published in a special issue of Spectroscopy - An International journal
(http://www.iospress.nl/site/navfr/navframe2.html)

Programme Structure:

The scientific programme will consist of invited lectures, as well as
contributed lectures selected from submitted contributions. There will
also be poster periods and time allocated for exhibition where
participants can discuss with spectroscopic manufacturers.

Deadlines:

Early Registration: 31 May 2002
Abstract Submission: 15 June 2002
Manuscript Submission for Publication in Spectroscopy: 8 July 2002

Conference Organising Committee Members :

Jose Luis Arrondo (University Pais Vasco, Spain)
Yoshinori Asakawa (Tokushima Bunri University, Japan)
Laurence D, Barron, (University of Glasgow, UK)
Andreas Barth (Johann Wolfgang Goethe Universitaet, Germany)
Lawrence J. Berliner (University of Denver, USA)
William Bonfield (University of Cambridge, UK)
Kevin M. Brindle (University of Cambridge, UK)
Chris Cooper (University of Essex, UK)
Gerard Cote (Texas A&M University, USA)
Lesley Davenport (CUNY, USA)
Martyn C Davies (University of Nottingham, UK)
Michael J. Davies (Heart Research Institute, Australia)
Peter J. Derrick (University of Warwick, UK)
David Gadian (Institute of Child Health, UK)
Parvez I. Haris (De Montfort University, UK)
Marcus A. Hemminga (Wageningen University, The Netherlands)
Larry Hench (Imperial College, London, UK)
Jan Johansson (Karolinska Institute, Stockholm, Sweden)
Mikio Kataoka (Nara Institute of Science & Technology, Japan)
Katrin Kneipp (Massachusetts Institute of Technology, USA)
Wlfgang Knoll (Max-Planck-Institut für Polymerforschung, Germany)
Kunihiro Kuwajima (University of Tokyo, Japan)
James M. McDonnell (University of Oxford, UK)
Henry H. Mantsch (Institute for Biodiagnostics, Canada)
Jeremy Nicholson (Imperial College, London, UK)
Niels Chr. Nielsen (University of Aarhus, Denmark)
Eric Oldfield (University of Illinois, USA)
Yukihiro Ozaki (Kwansei-Gakuin University, USA)
Hazime Saito (Himeji Institute of Technology, Japan)
Zhifeng Shao (University of Virginia School of Medicine, USA)
Gary Siuzdak (The Scripps Research Institute, USA)
Thomas G. Spiro (Princeton University, USA)
Gordon Tollin (University of Arizona, USA)
Bonnie Wallace (University of London, UK)

Conference Chair:

Dr P.I. Haris
Editor-in-Chief: Spectroscopy - An International Journal
http://www.iospress.nl/site/html/07124813.html
Editor: Biochemical Journal
http://www.biochemj.org/bj/bjedboard.htm
Editorial Advisor: Molecular Membrane Biology
http://www.tandf.co.uk/journals/boards/tmmb-edbrd.html
Treasurer: Protein and Peptide Science Group of Biochemical Society &
RSC
http://www.biochemistry.org/groups/ppsg/members.htm

Further information regarding the conference should be addressed to:

Dr Parvez I. Haris
Department of Biological Sciences,
De Montfort University, The Gateway, Leicester,
LE1 9BH, United Kingdom
E-Mail: pharis-at-dmu.ac.uk
Tel. 00-44-116-2506306 Fax: 00-44-116-2577287

REGISTRATION FORM:

Please send completed form by e-mail (pharis-at-dmu.ac.uk) to the
Conference Chair or by regular post: Dr P.I. Haris, Department of
Biological Sciences, De Montfort University, The Gateway, Leicester, LE1


9BH, United Kingdom, Fax: 00-44-116-2577287

Family name: .......................
Initials: .......................
First names: .......................
Institution: ..................................................
Department: ...............................................
Address: ....................................................
City/Postal Code: .............
Country: ..................
Telephone: .......................
Fax: .......................
E-mail: ........................................
Accompanying Person: .......................................

REGISTRATION FEES:

Amount
..........Early Registration. Payment received before 31 May 2002
(£400)
..........Late Registration. Payment received after 31 May 2002: (£500)
..........Student (£300)
..........Accompanying Person (£250)*
..........Total amount

Registration fee covers the following:
Accommodation for three nights in single rooms
Conference dinner
Lunch, tea, coffee for the duration of the conference
Conference bag, as well as the Abstract and Programme Book.
*Accompanying person registration fee covers accommodation for three
nights, conference dinner, lunch, tea and coffee.

PAYMENTS:

Registration fee can be directly paid by bank transfer: transfer should
be made to: 'Biospectra' Account, Account Number 02786668, Sort Code:
30-94-97, Lloyds TSB Bank, Leicester High Street Banch, Leicester, LE1
9FS, UK. The payments must be made in Sterling Pounds. Please state
your name and address.

Alternatively the registration fee can be paid by cheque or
international money order in Sterling Pounds, made payable to
'Biospectra'. The cheque should be sent by registered mail to Dr P.I.
Haris, Department of Biological Sciences, De Montfort University, The
Gateway, Leicester, LE1 9BH, United Kingdom.

Please send your payments by one of the above methods as soon as
possible. After receipt of your payment e-mail conformation will be
forwarded.

Accommodation:

Costs associated with accommodation are included within the registration
fee. Accommodation will be in single rooms for three nights covering
7-10 July 2002. Anyone requiring accommodation for additional nights
should contact the conference organiser in advance.

Information Regarding Arrival/Departure:

Date of arrival ........ / .......... / 2002
Date of departure ........ / .......... / 2002
*Need to be met at Cardiff Central Station: Yes/No
*Need to be met at Cardiff International Airport: Yes/No

*Please indicate time of arrival at the station or the airport by
e-mail.

Abstract Submission:

Any person registered to attend the conference may submit one Abstract
where he/she is the main author. It is possible submit additional
Abstracts where he/she is not the main author. The Organising committee
will select some of the Abstracts for oral presentation. Please
indicate if you are willing to present an oral presentation, if
selected.

The Abstract must be on one page, be single spaced and fit into an A4
size portrait-oriented rectangular space. Times New Roman fonts should
be used throughout the entire text of the abstract, including the title
and author sections. The name of the author who will present the paper
at the congress should be underlined. The Abstract should be prepared in
MS WORD for Windows. Abstracts should be sent by e-mail to
pharis-at-dmu.ac.uk or by regular post to the following address:
Dr P.I. Haris, Department of Biological Sciences, De Montfort
University, The Gateway, Leicester, LE1 9BH, United Kingdom. Fax:
00-44-116-2577287

Conference Publication:

Papers presented at the conference will be published in a special issue
of Spectroscopy - An International Journal. Manuscripts should be
submitted to the organisers at the Conference in Cardiff. Although
there are no specific length restrictions, papers should be of a length
appropriate to the material presented at the Conference. All papers
will be reviewed and are expected to be of a standard expected for a
paper published by the Journal in a regular issue.

The manuscripts should be prepared according to the instructions
available at http://www.iospress.nl/site/html/07124813_ita.html
The manuscript should be submitted at the conference in a diskette
containing the electronic version of the manuscript, together with two
paper copies.

The deadline for submission of manuscripts for publication in
Spectroscopy - An International Journal is 8 July 2002.

Exhibition:

Space will be reserved for exhibition of diverse spectroscopic
instrumentation, software, accessories, scientific journals and related
books. Companies interested in participating should take immediate
contact with the conference office.

Transportation:

Please let us know your travel plans so appropriate arrangements can be
made to meet you in either Cardiff Central Station or at Cardiff
International Airport.

London Heathrow Airport:

London's Heathrow Airport is the recommended route of arrival by air
into the UK. From here you can travel directly to Cardiff by National
Express coach (approximately 3 hours 15 minutes) or take the Heathrow
Express (15 minutes) to London's Paddington station and catch a First
Great Western The train journey to Cardiff Central station takes
approximately 2 hours. Members of the organising committee will be
available to meet the particpants at the Cardiff Central station.
Please indicate your arrival plans in advance.

London Gatwick Airport:

You can travel to Cardiff directly by National Express coach
(approximately 4 hours 45 minutes). Alternatively, you can take the
Gatwick Express train to London's Victoria Station (30 minutes) and
transfer to Victoria coach station from which the journey time is
approximately 3 hours 10 minutes. Alternatively from Victoria, use the
underground "Circle" line to reach Paddington Station and continue by
train as above.

London Stansted and London Luton Airports:

Budget flights are available from Europe into these airports which both
have rail and coach services into central London. For more information
on travel options visit the Stansted and Luton airport websites.

Cardiff International Airport:

Cardiff International Airport which is situated 12 miles west of the
city centre in Rhoose. Its main international connections are via
Amsterdam (KLM), Brussels and Paris (British Airways). British Airways
flights from Aberdeen, Edinburgh and Glasgow also serve the airport, as
well as Ryanair and British Airways flights from Dublin. Members of the
organising committee will be available to meet particpants arriving at
the airport. Please indicate your arrival plans in advance.

Eurostar/Train:

Arrival by Eurostar is to London Waterloo station. There is a direct
Great Western Train service from London Waterloo to Cardiff Central
Station with an approximate journey of 3 hours 10 minutes.
Alternatively, you may transfer at Waterloo to the underground
"Bakerloo" line to London Paddington where you can take the train as
above or take a coach from the Victoria station. Members of the
organising committee will be available to meet particpants arriving at
the Cardiff Central Station. Please indicate your arrival plans in
advance.

Information about Cardiff and Wales:

You can find out more details about Wales from various guides including
the Welsh Tourist Board (http://www.visitwales.com).

Cardiff, the capital city of Wales, boasts a number of tourist
attractions and has an extensive shopping centre. The civic centre
provides an interesting walking tour with many classical buildings
including Cardiff City Hall. Nearby are Cardiff Castle and the
Millennium Stadium. The National Museum (http://www.nmgw.ac.uk/) and
Gallery is also very popular with visitors. There are many eating and
drinking establishments situated in the city centre, ensuring that there
is something for everyone. Virtual Cardiff
(http://www.virtualcardiff.co.uk/) provides an indication of the wide
variety entertainment taking place in the city. Cardiff Bay, a
regenerated dock area, is a pleasant place to stroll around and can be
reached by a short train journey or 30 minutes walk from Cardiff City
centre. Cardiff, the Capital city is a lively, interesting and safe
city.

Further afield:

There are many interesting attractions in South Wales attractions
details of which can be found in various web-sites including Welsh
Tourist Board (http://www.visitwales.com). and Data Wales
(http://www.data-wales.co.uk/).

Activities:

Wales provides a wide variety of activities details of which can be
found in a number of websites including the Welsh Tourist Board
(http://www.visitwales.com). and Data Wales
(http://www.data-wales.co.uk/)

Useful Links:

Information regarding Wales: http://www.visitwales.com
Information Regarding Wales: http://www.data-wales.co.uk/
For UK train time tables visit www.railtrack.com
To book UK rail tickets outside the UK visit www.britcities.com
To book UK coach tickets visit www.gobycoach.com

Climate and Other Facts:

As Wales has a "temperate" climate, it generally never gets very hot or
very cold. July should be one of the sunniest and warmest months of the
year. However, it is advisable to bring a light rainproof coat and a
sweater, just in case, but you might possibly be in T-shirts. If you are
venturing into the mountains you will certainly need warmer clothing.
You can get a better idea nearer the time from the UK Meteorological
office (http://www.meto.gov.uk/).





From daemon Fri May 3 14:05:17 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 3 May 2002 13:05:09 -0600
Subject: Re: RONTEC USA Inc., Correction

Contents Retrieved from Microscopy Listserver Archives
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Unfortunately that is the case. Someone posts damaging material on this (or
any other) list, it get's sent around to some people a few times, and
suddenly it is "the official opinion of the microscopy specialists". I am
sure that can do substantial damage.

And it's not limited to the web or list servers. Audi (the German car
manufacturer) had a good business going in the US until someone decided to
start the engine and put it in forward (or backwards, I don't remember) and
run the car into a wall. There was a flaw, that allowed users to put the car
in gear without stepping on the brakes. Although that definitely was a flaw,
it was blown out of proportion and almost killed the Audi business in the
US. It took them 10 years to get their reputation back, even though I would
not consider this as dangerous as the tire problem that Firestone/Ford was
experiencing last year.

Bottom line: Everybody should be careful and check their sources when
posting potentially dmaging material on the listserver.

But that's just my 2 cents as a vendor.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Friday, May 03, 2002 6:40 AM
To: Microscopy-at-sparc5.microscopy.com


It's strange to see that one anonymous message could make so much damage to
the company. It seems to me that reputation of the company depends more on
the quality of the products/service etc nor on the one anonymous
opinion. From another hand, I think, this ListServer mostly is a place for
'customers' to exchange not only scientific ideas but to help each other to
survive in this 'capitalistic' world. Information about wrongdoing may
help others do not make similar mistake or spend money on bad quality
product/service. This is sort of our 'protection' against bad quality
service/products providers. Again, single decision even published here
could not damage the reputation of the good company with long well
established record of the service to EM community. I know, there are
places on the Internet where you could leave your opinion about some
particular business/company - it's perfectly legal and many institutions
used that lists for decision making. This discussion is more about ethics
- we all agree that we have rights openly speak here and others have the
rights to know who is speaking.

Sergey spoke.

At 02:15 AM 5/3/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri May 3 15:36:29 2002



From: Jo Dee Fish :      jfish-at-gladstone.ucsf.edu
Date: Fri, 3 May 2002 13:36:18 -0700
Subject: Bone prep for TEM

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Obviously, one can only conclude that the person(s) who sent this email are
competitors of RÖNTEC.


I am the owner of a small business & have always been in favor of fair
competition which benefits everyone.
Occasionally, we have someone resort to "badmouthing" us in order sway an
order.
I have never felt it necessary as we have always had an abundance of
business: relying on technical superiority & great customer relations.

I have found that equipment and Companies usually reflects the "personality"
of the people involved.
The same traits that I attribute to equipment (reliable, stable, solid)
reflects the personality of the people.
If the truth be known, I wonder about the integrity & reliability of
equipment the "anonymous Sender" may market.
It is obvious that he cannot sell based upon the equipment's technical
merits.

It is ashamed that a member of this Listserver would find it necessary to
resort to this "low-life" tactics.
I wonder if the anonymous Sender can look with pride at his accomplishments
& really take pride in himself for this feat.

My real name & my real Company for twenty years,

Earl Weltmer
Scanservice Corporation
----- Original Message -----
} From: Thomas Schülein
To: Microscopy-at-sparc5.microscopy.com
Sent: Friday, May 03, 2002 2:15 AM


Hello microscopists,
I am looking for a tried and true protocol for mouse bone. We are
interested in looking at the osteoblasts, and I am not sure how to
preserve them for examination of ultrathin sections in the TEM.
What is the best decalcification method? What type of resin for
embedding? Would a low viscosity resin, such as Spurr's, be the best?
I would appreciate any help you could give.
Thanks,
Jo Dee Fish

************************************************************
Jo Dee Fish
Research Technologist II
Microscopy Core
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 695-3720
Fax: (415) 285-5632
E-mail: jfish-at-gladstone.ucsf.edu

Mailing address:
Gladstone Institutes
P.O. Box 419100
San Francisco, CA 94141-9100
************************************************************


From daemon Fri May 3 18:26:36 2002



From: Rotole, John A. :      John.Rotole-at-ispat.com
Date: Fri, 3 May 2002 18:05:28 -0500
Subject: request for users manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning everyone,

I am trying to round up a copy of a users manual for the following software:

HP Data Translation DT VEE 3.0

(The latest version of this software is now called AGLIENT VEE Pro.)

If anyone can help me out, it would be much appreciated.

Thanks!

John

John A. Rotole, Ph.D.
Project Engineer
Coatings & Surface Technology
Ispat Inland Product Research
3001 E. Columbus Drive
East Chicago, Indiana 46312
Tel: 219-399-6308
Fax: 219-399-6562


From daemon Fri May 3 18:52:09 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 03 May 2002 16:45:25 -0700
Subject: Re: RONTEC USA Inc., Correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 01:28 PM 5/3/2002, you wrote:

[snip]


They were not totally anonymous since they had
a real(?) email address. A posting inquiry to that address
did not generate any response. So your point is
well taken.

gg



From daemon Fri May 3 22:23:22 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 3 May 2002 23:19:54 -0400
Subject: Question on EDX spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;

On a few occasions I have been handed reports from outside our organization
with EDX spectra that were saved as image files, e.g. .bmp. .tif, .jpg etc.
and pasted into a Word document or another report format. However, this
"cut and paste job" is only an image and in my mind isn't data at all but
simply information that can't be verified or refuted. A case in point is
when I received a spectra that only displayed energies up to 3.5 KeV and the
report identified Pt as Au which overlap at the lower energy region but
spread out higher up. Now without the raw data I cannot do anything but
take someone's word or politely interrogate the person that did the
analysis. My question is whether it would be appropriate to ask someone on
this listserver with the same EDX system to look at the raw data, if
available, provided they have the same platform?

These matters are generally not life and death like a forensics issue but
purely economic, but I'd rather be in jail than lose money.

Regards,
Peter


From daemon Sat May 4 08:17:26 2002



From: zaluzec-at-microscopy.com
Date: Sat, 4 May 2002 08:07:34 -0500
Subject: Re: Question on EDX spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter

The task of sharing spectra cross-platform was address a number
of years ago by the MSA/MAS Standards Committee.

You could ask the individual to send the raw data to you
in the MSA/MAS File format. You can then look at it on
any computer with something as simple as a spread sheet
program. In addition, a number of commerical manufacturers
have implemented a routine to read/write data to/from this
format, hence you might be able to look at the raw data on
your own system.

You can find the details on the MSA/MAS format for Spectra at

ftp://www.amc.anl.gov/AMC-3/ANLSoftwareLibrary/2-EMMPDL/Eels/emmff/


Nestor
Your Friendly Neighborhood SysOp


========================
Keywords :XEDS,EELS,AES,WDS,CLS,GAM,XRF,PES
Computer :IBM, MAC, DEC
Operating System :ALL
Programming Language :Fortran 77
Hardware Requirements :None
Author(s) :EMSA/MAS TASK FORCE
Ray Egerton ,Charles E. Fiori ,John A. Hunt,
Mike S. Isaacson,Earl J. Kirkland ,Nestor J. Zaluzec
Correspondence Address :R.F. EGERTON-CHAIRMAN
University of Alberta
Dept. of Physics
Edmonton, Alberta, Canada, T6G2J1
Abstract:

A simple format for the exchange of digital spectral data is
presented, and proposed as an EMSA/MAS standard. This format is readable by
both humans and computers and is suitable for transmission through various
electronic networks (BITNET, ARPANET), the phone system (with modems) or on
physical computer storage devices (such as floppy disks). The format is not
tied to any one computer, programming language or computer operating system.
The adoption of a standard format would enable different laboratories to freely
exchange spectral data, and would help to standarize data
analysis software. If equipment manufacturers were to support a common format,
the microscopy and microanalysis community would avoid duplicated effort in
writing data-analysis software. This version of EMSAMASFF contains two
subroutines which read and write spectral data files Version 1.0 data format.
The data are stored as simple ASCII characters at a user defined number of
columns per line for the length of the data file. The spectral data is
preceeded by a series of header lines, which tell the user about the
parameters of the spectrum. The header lines are identified by the first
character in the line being the symbol (#) followed by a descriptor and if
appropriate its units. An example of a data file format can be found in the
EMSAMASFF.DOC file.


From daemon Sat May 4 11:56:04 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Sat, 04 May 2002 09:43:46 -0700
Subject: Re: Bone prep for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use EDTA with NaOH to decalcify, it's slow and you have to keep it cold,
but it is gentle. Then dehydrate and infiltrate with long spells in each
change, and embed in a hard resin - I use Epon with DDSA and NMA and, to
embed, 2% DMP30. Use vacuum or a rotator (or both in turn) to force the
resin in, and after embedding leave it overnight at 37C, if possible under
vacuum (I use an old paraffin oven) before putting it into the 60C oven.
People do use microwave techniques, but I never had much luck with them.
Hope this helps.

Lesley Weston.


on 03/05/2002 1:36 PM, Jo Dee Fish at jfish-at-gladstone.ucsf.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello microscopists,
} I am looking for a tried and true protocol for mouse bone. We are
} interested in looking at the osteoblasts, and I am not sure how to
} preserve them for examination of ultrathin sections in the TEM.
} What is the best decalcification method? What type of resin for
} embedding? Would a low viscosity resin, such as Spurr's, be the best?
} I would appreciate any help you could give.
} Thanks,
} Jo Dee Fish
}
} ************************************************************
} Jo Dee Fish
} Research Technologist II
} Microscopy Core
} Gladstone Institute of Cardiovascular Disease
}
} Telephone: (415) 695-3720
} Fax: (415) 285-5632
} E-mail: jfish-at-gladstone.ucsf.edu
}
} Mailing address:
} Gladstone Institutes
} P.O. Box 419100
} San Francisco, CA 94141-9100
} ************************************************************
}



From daemon Sat May 4 15:49:35 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Sat, 4 May 2002 16:25:05 -0400
Subject: Question on EDX spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think that you have a need to trust your analyst to know what he/she is doing. Unfortunately, in the age of push button data collection on instruments that anyone can use, the training may or may not be up to snuff. Of course, you can rely on "certified" labs that are ISO-9000 qualified, but if you do, they may be just following a rote script. It is best to know that analyst's pedigree and their experience level.



-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Friday, May 03, 2002 11:20 PM
To: Microscopy-at-sparc5.microscopy.com


Folks;

On a few occasions I have been handed reports from outside our organization
with EDX spectra that were saved as image files, e.g. .bmp. .tif, .jpg etc.
and pasted into a Word document or another report format. However, this
"cut and paste job" is only an image and in my mind isn't data at all but
simply information that can't be verified or refuted. A case in point is
when I received a spectra that only displayed energies up to 3.5 KeV and the
report identified Pt as Au which overlap at the lower energy region but
spread out higher up. Now without the raw data I cannot do anything but
take someone's word or politely interrogate the person that did the
analysis. My question is whether it would be appropriate to ask someone on
this listserver with the same EDX system to look at the raw data, if
available, provided they have the same platform?

These matters are generally not life and death like a forensics issue but
purely economic, but I'd rather be in jail than lose money.

Regards,
Peter


From daemon Sat May 4 17:32:32 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Sat, 4 May 2002 18:29:04 -0400
Subject: Question on EDX spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott;

Unfortunately sometimes the "analyst" is a few people removed in the food
chain of the organization I may have to deal with and that's sometimes a
customer so I must at all times be cordial and diplomatic. It turned out
that the individual that sent me the "pictorial" spectra, but no raw data,
was a "push button" let the machine identify everything type of analyst and
was not very conversant in where error comes from in EDX analyses. He also
had no real understanding of the device he was "analyzing" so I should
probably be a bit more forgiving. I would have preferred that his report
stated "these are the possible elements detected, Au, Pt etc."

I must take this opportunity to agree with you on ISO9000 certification and
speak my mind. ISO is not an education in engineering or science, it is an
education on how to be compliant with paperwork. If one documents a bad
recipe for a cake, one will get a consistently bad cake but will not be on
The Martha Stewart Show. I often wonder how many organizations filed for
bankruptcy but had wonderful ISO documentation and passed all their audits?
I really should look this up somewhere.

I hope my boss doesn't subscribe to this, naaaaah.

Peter

-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Saturday, May 04, 2002 4:25 PM
To: 'Peter Tomic'
Cc: Microscopy (E-mail)


Folks;

On a few occasions I have been handed reports from outside our organization
with EDX spectra that were saved as image files, e.g. .bmp. .tif, .jpg etc.
and pasted into a Word document or another report format. However, this
"cut and paste job" is only an image and in my mind isn't data at all but
simply information that can't be verified or refuted. A case in point is
when I received a spectra that only displayed energies up to 3.5 KeV and the
report identified Pt as Au which overlap at the lower energy region but
spread out higher up. Now without the raw data I cannot do anything but
take someone's word or politely interrogate the person that did the
analysis. My question is whether it would be appropriate to ask someone on
this listserver with the same EDX system to look at the raw data, if
available, provided they have the same platform?

These matters are generally not life and death like a forensics issue but
purely economic, but I'd rather be in jail than lose money.

Regards,
Peter


From daemon Sat May 4 19:07:50 2002



From: Barton Smith :      smithdb-at-ornl.gov
Date: Sat, 04 May 2002 19:58:05 -0400
Subject: Re: EM-A question for AN10000 owners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:01 PM -0700 on 5/3/02 you wrote:
} Hi All:
} I am trying to convert an image and spectra from the Oxford AN 10000 EDS
} to a .tiff that is readable on a PC. I've done it in the past, but it has
} been so long, I forget the exact procedure. I hope that there is
} somone out there that does this more regularly that can refresh my
} memory.
} Thanks in advance,
} Mike Coviello
} UT Arlington


Procedure for converting AN10000 spectrum screendumps to TIFF format
files on PC DOS disks

1) From the Analyser MAIN MENU select (4) PRINT/PLOT

2) Select (3) DUMP TO DISK
Enter filename FILENAME.IM
Message: File FILENAME.IM does not exist in STAFF/STUDENT.DR
Do you wish to create it? Press Y
The spectrum will now be saved to the working directory as FILENAME.IM

3) Return to the GO menu

4) Select (4) FILE MANAGEMENT

5) Select (16) DEMON/TIFF convert

6) Select (1) IMAGE filename. Enter FILENAME.IM

7) Select (2) TIFF filename. Enter FILENAME.TI

8) Select (3) LOOKUP table: MSDOSCV.LT

9) Select (8) IMAGE to TIFF
Overwrite file if it exists: Yes
Rescale pixels for grey image: Yes

10) Select (10) MSDOS convert
Insert DOS formatted disk in DM0
Source disk for DOS: DM0

11) Select (1) CONVERT files
Copy file to (MSDOS/DEMON): MSDOS
Filename please: FILENAME.TI
Any conversion type: B
Transfer will now take place, lasting approximately 3
minutes. The TIFF file will be a
grey-scale image. Use an image processing package to
change it to color.

12) Select (3) DEMON utilities

13) Select (2) DELETE DEMON files
Enter FILENAME.-
Message: Delete DEMON file FILENAME.TI? Press Y
Message: Delete DEMON file FILENAME.IM? Press Y
Press Blue

14) Select (20) EXIT

15) Select (20) EXIT



--
Barton Smith, Ph.D.
Advanced Lasers and Optics Group
Engineering Science and Technology Division
Oak Ridge National Laboratory


From daemon Sun May 5 04:22:01 2002



From: Allen Sampson :      ars-at-sem.com
Date: Sun, 5 May 2002 04:11:02 -0700
Subject: RE: Question on EDX spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If your opinion is being sought, then you should have available all raw
data. Whether it is a legalistic matter or not, a definitive opinion can
not be rendered on a synopsis of available data, only on original data.

Preferably, the original sample would be made available to you for
analysis. In this way, not only the interpretation, but also the
providence of that interpretation would be verifiable by you.

Your query whether it is appropriate to find someone with the same
equipment is moot. It is the interpretation of the results given the
analytical conditions that is far more important than the data delivered.
The data as delivered to you has already undergone the filters of the one
who sent it to you. You need to see past that and provide your own
interpretation of the truth, which can only be based on data that you know
to be based on some semblance of reality. Since the data collection of EDX
spectra is based on a variety of experimental conditions, the better they
are known, the better they can be corrected for.

In other words, you need to know not only the raw data but also the
experimental conditions it was collected under as well as the providence of
the sample. To provide an opinion on anything less would be a disservice
to those who ask as well as those who oppose the requesting party.
Assuming the desire to be an impartial source, there is no other course
than to do your own analysis..


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


}
} Folks;
}
} On a few occasions I have been handed reports from outside our
organization
} with EDX spectra that were saved as image files, e.g. .bmp. .tif, .jpg
etc.
} and pasted into a Word document or another report format. However, this
} "cut and paste job" is only an image and in my mind isn't data at all but
} simply information that can't be verified or refuted. A case in point is
} when I received a spectra that only displayed energies up to 3.5 KeV and
the
} report identified Pt as Au which overlap at the lower energy region but
} spread out higher up. Now without the raw data I cannot do anything but
} take someone's word or politely interrogate the person that did the
} analysis. My question is whether it would be appropriate to ask someone
on
} this listserver with the same EDX system to look at the raw data, if
} available, provided they have the same platform?
}
} These matters are generally not life and death like a forensics issue but
} purely economic, but I'd rather be in jail than lose money.
}
} Regards,
} Peter
}



From daemon Sun May 5 06:57:11 2002



From: barbara maloney :      bmalon01-at-fiu.edu
Date: Sun, 05 May 2002 07:47:16 -0400
Subject: grainy negative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karli - by chance could your column be containmented or have a whisker?
I know sometimes if your image isn't good sometimes your filament could
be going, but probably not for weeks. Just a thought.
Barb



From daemon Sun May 5 14:35:37 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 05 May 2002 12:21:04 -0700
Subject: DEC M7555 card

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Disclaimer: This is not a for sale posting.

I ran across an LSI-11 bus M7555 card which
seems to be in good condition. No idea what it
is or if it works. It has a ribbon connector (1)
on the pull handles end. It is a DEC card.

Free to a good home.

gary g.



From daemon Sun May 5 18:55:03 2002



From: rahul padmakar panat :      panat-at-students.uiuc.edu
Date: Sun, 5 May 2002 21:11:26 -0500 (CDT)
Subject: Vacuum high Temperature experiment

Contents Retrieved from Microscopy Listserver Archives
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Allen;

Thank you. I am saving this email for reference in my next argument with
upper management.

Peter

-----Original Message-----
} From: Allen Sampson [mailto:ars-at-sem.com]
Sent: Sunday, May 05, 2002 7:11 AM
To: 'Peter Tomic'; Microscopy-at-sparc5.microscopy.com



Hi all,

I have a specific question regarding experiments in inert environment:

The experiment I did was: A small superalloy (SA) sample was taken
in a quartz tube and I placed the tube horizontally in a tube furnace. I
then ran Ar gas for a few minutes through it and under the assumption
that all oxygen is gone, heated the tube to 1200 C, held there for 50
min before cooling the apparatus to 200C. This I thought would avoid
any oxidation of the SA sample.
On cooling, to my disappointment, the SA surface
oxidized (shiny surface became black). In fact, the quartz tube became
black from inside. Note that throughout the experiment, I had inserted
a thermocouple in the quartz tube, which (the thermocouple) had a Nextel
Ceramic fiber insulation. This insulation also turned black.

The specific questions in my mind are:
1) How do I make sure that all the oxygen is flushed out? Is a
vacuum necessary before Ar gas is filled in the tube?
2) In my experiment, did the Thermocouple insulation emit any gas (from
the black color of the quartz tube and the nextel insulation)?
In that case, can i have a bare theomocouple wire in the tube at
1200C?

Thanks in advance

Rahul Panat
Dept. of Theoretical and Applied Mechanics
Univ of Illinois,
Urbana, IL




From daemon Sun May 5 22:50:44 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Sun, 05 May 2002 20:40:30 -0400
Subject: Re: Vacuum high Temperature experiment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 5/5/02 10:11 PM, rahul padmakar panat at panat-at-students.uiuc.edu wrote:

}
} I have a specific question regarding experiments in inert environment:
}
} The experiment I did was: A small superalloy (SA) sample was taken
} in a quartz tube and I placed the tube horizontally in a tube furnace. I
} then ran Ar gas for a few minutes through it and under the assumption
} that all oxygen is gone, heated the tube to 1200 C, held there for 50
} min before cooling the apparatus to 200C. This I thought would avoid
} any oxidation of the SA sample.
} On cooling, to my disappointment, the SA surface
} oxidized (shiny surface became black). In fact, the quartz tube became
} black from inside. Note that throughout the experiment, I had inserted
} a thermocouple in the quartz tube, which (the thermocouple) had a Nextel
} Ceramic fiber insulation. This insulation also turned black.
}
} The specific questions in my mind are:
} 1) How do I make sure that all the oxygen is flushed out? Is a
} vacuum necessary before Ar gas is filled in the tube?
} 2) In my experiment, did the Thermocouple insulation emit any gas (from
} the black color of the quartz tube and the nextel insulation)?
} In that case, can i have a bare theomocouple wire in the tube at
} 1200C?
}
} Thanks in advance
}
} Rahul Panat
}
Dear Rahul,
If you had a long, thin quartz tube, the Ar would not necessarily flush
out all the O2, so, yes, I would evacuate the tube furnace, flush with Ar,
repeat until all the O2 is gone. (I'm thinking that the tube was not open
at both ends, and that the Ar was not directed at the end of the tube.) I'd
try the experiment again without the SA, but with a clean quartz tube and
new TC; if they again turn black, then it's something in the TC insulation.
If not, try again with the SA, and see if anything unexpected occurs. Good
luck.
Yours,
Bill Tivol



From daemon Mon May 6 00:51:17 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Mon, 6 May 2002 07:42:49 +0200
Subject: Flat embedding of LR White

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ann Fook

We normally imbed in gelatin capsules. Polymerise in a oven. Use a hacksaw
(Very small) to cut and re-glue our specimens in the preferred orientation
before sectioning. That way our orientation is perfect every time!

Just a useful hint.


Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana

Phone : +267 355 2426
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw


-----Original Message-----
} From: Ann-Fook Yang [mailto:yanga-at-EM.AGR.CA]
Sent: Friday, May 03, 2002 5:26 PM
To: microscopy-at-sparc5.microscopy.com


Hi everyone,

I need to do flat embedding using LR White. A Teflon mold covered with
ACLAR seems to be the answer. I am afraid that the over filled resin under
ACLAR may not polimerize properly and become messy. I appreciate your
experience.

Ann Fook Yang



From daemon Mon May 6 01:54:04 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Mon, 6 May 2002 16:49:54 +1000
Subject: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

We have a Jeol 100CX TEM that, I am told, has developed the following
vacuum problem over the last 5-7 years. The problem, whatever its cause,
means that the viewing port into the observation chamber has to be removed
once a month or so to clean off the buildup of oil on the inside. I am
amazed that this was considered Ok for the microscope........ So, my
proposal is to replace all the rubber vacuum lines, and clean out, as best
as possible, the remaining components - metal hoses, etc. We're not sure
of the cause of this problem, whether it's oil from the rotary pumps or
from one of the diffusion pumps. Oh, and we need to clean, or at least
check, the column as well, of course......

My questions are:
Can we replace the original rubber tubing with wire-reinforced clear PVC
tubing (at least then we'd see any oil buildup in future)? It's a little
unclear how much vacuum the PVC tubing will take and this will be important
in the internals of the microscope.

How can we identify the source of the problem? I'm proposing to put
foreline traps on both rotary pumps, but if a diff pump is the problem,
this won't solve it. I'm also proposing to take out the diff pumps and
check them, change the oil, etc. I've had a look through Will Bigelow's
Vacuum Methods book (very useful!), and it's a bit like reading an index of
diseases and their symptoms - you think you have a whole variety of them!!
- I can see several incidents and problems that could have affected a
number of the vacuum components.


Thanks for any and all advice!
cheers,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au




From daemon Mon May 6 05:13:00 2002



From: Allen Sampson :      ars-at-sem.com
Date: Mon, 6 May 2002 05:04:13 -0700
Subject: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, a changeover to the clear PVC hosing is appropriate - I love the
stuff. The problem you are experiencing is probably more a consequence of
the calibration of the vacuum system than anything else.

Anyone who has been here for awhile has probably seen postings from me on
the vacuum system calibrations of EMs regarding the cross-over points of
diffusion pumped systems. Too often, design engineered or field
engineering solutions result in a cross-over vacuum level from roughing to
diffusion pumps at too high a pressure level. When a diffusion pump is
opened to a pressure level too high, it can stall which results in the
breakdown of the normal laminar flow of oil vapor to a chaotic flow which
results in the influx of oil vapor into the sample chamber.

Your first step should be to determine the actual chamber pressure at which
the cross-over from roughing to diffusion pump occurs. At the least, you
can get a qualitative determination from the action of the chamber vacuum
when the diffusion pump kicks in. At that point, the vacuum level should
start a rapid increase to the ultimate vacuum. If, instead, the vacuum
level declines or holds steady for a few seconds, the cross-over point is
too high and the diffusion pump is stalling. Cross-over should normally
occur at 70 - 100 microns, most diffusion pumps will react well in this
area. I generally tend to set the cross-over at 70 microns - it will take
longer for the roughing time but when the diffusion pump kicks in it will
rapidly pull down to the ultimate vacuum and the sample chamber will remain
cleaner.

Field engineers don't normally carry independant vacuum measuring gear.
Instead, they depend on known timing characteristics of known good
systems. If your system doesn't match the characteristics expected, the
calibration will be off. The only offset to this is the use of a leak back
test, where the actual leak rate of the system can be tested. If the leak
back can be verified as within the manufacturer's specs, then the
calibration can be applied and expected to achieve specs.

If, however, there is no absolute measurement made of either actual vacuum
levels or actual leak rates, then no assumptions can possibly be made of
the vacuum levels achieved. Turbo-pumping systems actually make this
determination of vacuum levels more problematic as they are seldom capable
of closing a valve on the sample chamber to allow the determination of its
leak rate, making the use of accurate vacuum level measuring equipment
neccessary to determining the the calibration of system levels.



Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Sunday, May 05, 2002 11:50 PM, Rosemary White
[SMTP:rosemary.white-at-csiro.au] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} We have a Jeol 100CX TEM that, I am told, has developed the following
} vacuum problem over the last 5-7 years. The problem, whatever its cause,
} means that the viewing port into the observation chamber has to be
removed
} once a month or so to clean off the buildup of oil on the inside. I am
} amazed that this was considered Ok for the microscope........ So, my
} proposal is to replace all the rubber vacuum lines, and clean out, as
best
} as possible, the remaining components - metal hoses, etc. We're not sure
} of the cause of this problem, whether it's oil from the rotary pumps or
} from one of the diffusion pumps. Oh, and we need to clean, or at least
} check, the column as well, of course......
}
} My questions are:
} Can we replace the original rubber tubing with wire-reinforced clear PVC
} tubing (at least then we'd see any oil buildup in future)? It's a little
} unclear how much vacuum the PVC tubing will take and this will be
important
} in the internals of the microscope.
}
} How can we identify the source of the problem? I'm proposing to put
} foreline traps on both rotary pumps, but if a diff pump is the problem,
} this won't solve it. I'm also proposing to take out the diff pumps and
} check them, change the oil, etc. I've had a look through Will Bigelow's
} Vacuum Methods book (very useful!), and it's a bit like reading an index
of
} diseases and their symptoms - you think you have a whole variety of
them!!
} - I can see several incidents and problems that could have affected a
} number of the vacuum components.
}
}
} Thanks for any and all advice!
} cheers,
} Rosemary
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} 61- 2 6246 5475 or
} 61- 0402 835 973
} rosemary.white-at-csiro.au
}
}
}
}
}



From daemon Mon May 6 06:04:52 2002



From: colleen_cockrell-at-aSurfer.com
Date: Mon, 06 May 2002 14:53:43 -0400
Subject: please read

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Mon May 6 08:09:04 2002



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Mon, 6 May 2002 09:49:47 -0400
Subject: Re: Vacuum high Temperature experiment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists & Co.;

I received the below notice from our friendly IT people. I believe it was
incoming from the listserver and I though you may want to be cautious about
the "sender."

Regards,
Peter

-----Original Message-----
} From: MB10190 [mailto:MB10190-at-aol.com]
Sent: Sunday, May 05, 2002 1:40 PM
To: PTomic-at-anadigics.com



I would add; verify purity of your argon or use an argon purifier.


At 8:40 PM -0400 5/5/02, Bill & Sue Tivol wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html


From daemon Mon May 6 09:14:22 2002



From: gary.m.brown-at-exxonmobil.com
Date: Mon, 6 May 2002 09:05:59 -0500
Subject: Re: Flat embedding of LR White

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Ann-Fook,

My approaches in using LRWhite differ by the nature of the sample being
embedded:
Hard samples, such as silica and zeolites, are embedded in LRWhite (hard
grade) without accelerator. The sample is first vacuum degassed,
immersed in LRWhite in the mold and placed under vacuum again to
facilitate complete infiltration of the resin into the sample. The
embedded sample is then cured overnight at 75-90C. This gives a hard,
brittle block that is ideal for sectioning hard samples.
I try to avoid embeddment of polymers in LRWhite. Some polymers may be
partially to very soluble in the acrylic monomer and thus susceptible to
swelling by the resin. Furthermore, since the heat of curing is not
known to me, I choose not to take chances on the annealing samples
during exothermic curing. Rather, I generally choose to use one of the
generic Epon 812 replacements for these materials. Carefully controlled
curing conditions allow embedment and microtomy without concerns of
annealing.
This said, if I must embed polymers in LRWhite, I choose the correct
hardness of resin (soft, medium or hard) for the material being
embedded. The embedding mold is degassed for a bit under a nitrogen flow
and the mold surfaces are then wiped with the accelerant on a cotton
swab. This helps the resin in contact with the mold surface to more
fully cure. The samples are embedded in the resin/accelerator and
immediately placed under nitrogen flow until curing is complete.

Good luck,

Gary M. Brown





"Ann-Fook Yang"
{yanga-at-EM.AGR.C To: {microscopy-at-sparc5.microscopy.com}
A} cc:
Subject: Flat embedding of LR White

05/03/02 10:26
AM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi everyone,

I need to do flat embedding using LR White. A Teflon mold covered with
ACLAR seems to be the answer. I am afraid that the over filled resin under
ACLAR may not polimerize properly and become messy. I appreciate your
experience.

Ann Fook Yang








From daemon Mon May 6 09:42:07 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Mon, 06 May 2002 16:15:59 -0400
Subject: Re: confocal--overlapping signal problem?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm glad someone else got around to this before I did! As
you can see, it's rather involved. I will add two
additional points:

1) One of my AN10000's doesn't have DEMON/TIFF convert as
an option (older software). I must use the "newer" version
of the QX200 Utilities software.
2) At step 9 below: both answers must be an uppercase 'Y'.
Again, possibly peculiar to my system/version.
Good luck!

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Saturday, May 04, 2002 7:58 PM, Barton Smith
[SMTP:smithdb-at-ornl.gov] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} ml
}
----------------------------------------------------------
} -------------.
}
}
} At 12:01 PM -0700 on 5/3/02 you wrote:
} } Hi All:
} } I am trying to convert an image and spectra from the
} } Oxford AN 10000 EDS
} } to a .tiff that is readable on a PC. I've done it in
the
} } past, but it has
} } been so long, I forget the exact procedure. I hope that
} } there is
} } somone out there that does this more regularly that can
} } refresh my
} } memory.
} } Thanks in advance,
} } Mike Coviello
} } UT Arlington
}
}
} Procedure for converting AN10000 spectrum screendumps to
} TIFF format
} files on PC DOS disks
}
} 1) From the Analyser MAIN MENU select (4) PRINT/PLOT
}
} 2) Select (3) DUMP TO DISK
} Enter filename FILENAME.IM
} Message: File FILENAME.IM does not exist in
} STAFF/STUDENT.DR
} Do you wish to create it? Press Y
} The spectrum will now be saved to the working directory
} as FILENAME.IM
}
} 3) Return to the GO menu
}
} 4) Select (4) FILE MANAGEMENT
}
} 5) Select (16) DEMON/TIFF convert
}
} 6) Select (1) IMAGE filename. Enter FILENAME.IM
}
} 7) Select (2) TIFF filename. Enter FILENAME.TI
}
} 8) Select (3) LOOKUP table: MSDOSCV.LT
}
} 9) Select (8) IMAGE to TIFF
} Overwrite file if it exists: Yes
} Rescale pixels for grey image: Yes
}
} 10) Select (10) MSDOS convert
} Insert DOS formatted disk in DM0
} Source disk for DOS: DM0
}
} 11) Select (1) CONVERT files
} Copy file to (MSDOS/DEMON): MSDOS
} Filename please: FILENAME.TI
} Any conversion type: B
} Transfer will now take place, lasting approximately 3
} minutes. The TIFF file will be a
} grey-scale image. Use an image processing
} package to
} change it to color.
}
} 12) Select (3) DEMON utilities
}
} 13) Select (2) DELETE DEMON files
} Enter FILENAME.-
} Message: Delete DEMON file FILENAME.TI? Press Y
} Message: Delete DEMON file FILENAME.IM? Press Y
} Press Blue
}
} 14) Select (20) EXIT
}
} 15) Select (20) EXIT
}
}
}
} --
} Barton Smith, Ph.D.
} Advanced Lasers and Optics Group
} Engineering Science and Technology Division
} Oak Ridge National Laboratory


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Dear Rahul Panat

If it is possible link the tube to a vacuum system it will help. Flushing
with Ar a few times will improve it dramatically. It is worth to allow a
very slow flow of Ar during heating and cooling over your sample. This is
done by closing both ends of your quarts tube with a inlet and outlet pipe.
The outlet pipe is run into a holder with water. Increase the Ar flow until
a very small bubble rate is achieved. This will ensure a inert atmosphere
with a positive Ar pressure.

Yours Sincerely
Stephan H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana


Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}

Hi all,

I have a specific question regarding experiments in inert environment:

The experiment I did was: A small superalloy (SA) sample was taken
in a quartz tube and I placed the tube horizontally in a tube furnace. I
then ran Ar gas for a few minutes through it and under the assumption
that all oxygen is gone, heated the tube to 1200 C, held there for 50
min before cooling the apparatus to 200C. This I thought would avoid
any oxidation of the SA sample.
On cooling, to my disappointment, the SA surface
oxidized (shiny surface became black). In fact, the quartz tube became
black from inside. Note that throughout the experiment, I had inserted
a thermocouple in the quartz tube, which (the thermocouple) had a Nextel
Ceramic fiber insulation. This insulation also turned black.

The specific questions in my mind are:
1) How do I make sure that all the oxygen is flushed out? Is a
vacuum necessary before Ar gas is filled in the tube?
2) In my experiment, did the Thermocouple insulation emit any gas (from
the black color of the quartz tube and the nextel insulation)?
In that case, can i have a bare theomocouple wire in the tube at
1200C?

Thanks in advance

Rahul Panat
Dept. of Theoretical and Applied Mechanics
Univ of Illinois,
Urbana, IL




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Pauline,
Try decreasing the brighter labels concentration. Also run single label
counterparts as bleed through
controls (same concentrations as the double label, but use only the fitc and
PI separately). If you get
bleed with the single label samples (capture with both channels), you can
then do a backround subtract
to get the real signal. Good luck

Mike D.

pcy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We have stained annelid embryos with FITC-conjugated anti-tubulin antibody
} and propidium iodide. We are using a Biorad-MRC-600 and seem to be getting
} almost identical signals(of nuclei and a few mitochondria) on both the 488
} and 568 wavelengths, even though when viewed with epifluorescence, the
} tubulin stain seems to distinctly define cell borders. I understood that
} the propidium iodide emission would not be overlapping with that of
} FITC--is this incorrect? I have other double-labelled samples which are
} stained with different stains, but their signals are within the
} appropriate emission spectra for both confocal and epifluorescence, so the
} detection filters on the confocal do not seem to be the problem.
}
} Any feedback is appreciated!
}
} Pauline Yu
} pcy-at-usc.edu
} Manahan Lab
} http://www.usc.edu/manahanlab



From daemon Mon May 6 16:38:28 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 06 May 2002 14:42:09 -0700
Subject: Fwd: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Date: Mon, 06 May 2002 14:39:09 -0700
} To: "ars-at-sem.com" {ars-at-sem.com}
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: RE: cleaning vacuum system
}
} The only things you have to keep in mind: PVC tubes are less flexible and
} may 'transfer' vibration from rotary pump to the microscope's column. For
} this reason, I love to use PVC on vacuum evaporators nor the microscopes.
}
} As for oil in the column - to me it looks like DP problem and water
} waffler, which is situate over DP. The reason I think so, that usually we
} do not pump columns down by RP very often. It happening on my microscope
} a few times over 15 years, I believe. So, it mean, that RP do not touch
} column frequently (except terrible downstream if it happening). I do
} believe that 100C/CX DPs have water wafflers, check does water go
} through. Another things: if DP oil is old (or overheated over some period
} of time) it may be deteriorated and boiling point may decreased (shorter
} molecules), so some low-weight components may not condensate effectively
} and will migrate into the column. I would suggest to check DP oil and
} replace it on Santovak-5 like type (and forget for 5+ years). If you
} decided to replace DP oil, I would suggest, you install oil traps on RPs
} and new tubings as well to protect fresh oil (you never know...). Good
} luck! Sergey
}
} At 05:04 AM 5/6/02 -0700, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 6 16:47:33 2002



From: A.P Alves de Matos :      apamatos-at-oninet.pt
Date: Mon, 6 May 2002 23:06:49 +0100
Subject: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After all the cleaning, I would check the water lines & the water
temperature.

I have had problems similar to the one you describe. It turned out that
after moving the EM the input & output lines were reversed causing oil
backstreaming. In the second case, the water temperature had increased
overnight as it was connected to the tap water.

Another thought would be to check the pre-pump pressure to ensure that it is
switching over at the correct pressure.

Regards,


Earl





----- Original Message -----
} From: "Rosemary White" {rosemary.white-at-csiro.au}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, May 05, 2002 11:49 PM


Dear Rosemary

Once, in the darkness of time (1975-...), I used one of the first JEOL 100C
microscopes. That machine also developed with time severe contamination
problems that, I believe, may be similar to the ones you mention.
As far as we could find out, and as far as I can recall (forgive some
possible innacuracies) the trouble came from the particular design of the
vacum system, in which two diffusion pumps are linked together, the exit of
the upper pump being connected to the observation chamber. In these early
microscopes, that design seemed to lead to oil reflux from the exit of the
upper pump into the column, at least during some stages of the vacum
sequence. One cure for the problem was to close permanently the valve
connecting the observation chamber with the vacuum system. That was the only
measure that worked! Enough vacuum could still be achieved by the upper
connection alone.
As far as I was told newer microscopes (yours should be one of these) were
built with an improved automated vacuum sequence and traps that avoided that
problem. My guess is that either your oil traps are not operational
(obstructed water circulation?), either the vacuum sequence is incorrect
(check the pirani gauge settings that control the sequence against true
vacum values if you are able to measure them!). Some vacuum valve (the lower
connection to the column should be the primary suspect) may be stuck, or
electronics is producing a wrong sequence etc...
Anyhow if you have this problem, continuos cleaning does not seem to be an
answer. It was not for me.

Best wishes and good luck
Prof. Doctor A.P. Alves de Matos
Anatomic Pathology Department
Curry Cabral Hospital
Lisbon


-----Mensagem original-----
De: Rosemary White [mailto:rosemary.white-at-csiro.au]
Enviada: Segunda-feira, 6 de Maio de 2002 7:50
Para: Microscopy-at-sparc5.microscopy.com
Assunto: cleaning vacuum system


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,

We have a Jeol 100CX TEM that, I am told, has developed the following
vacuum problem over the last 5-7 years. The problem, whatever its cause,
means that the viewing port into the observation chamber has to be removed
once a month or so to clean off the buildup of oil on the inside. I am
amazed that this was considered Ok for the microscope........ So, my
proposal is to replace all the rubber vacuum lines, and clean out, as best
as possible, the remaining components - metal hoses, etc. We're not sure
of the cause of this problem, whether it's oil from the rotary pumps or
from one of the diffusion pumps. Oh, and we need to clean, or at least
check, the column as well, of course......

My questions are:
Can we replace the original rubber tubing with wire-reinforced clear PVC
tubing (at least then we'd see any oil buildup in future)? It's a little
unclear how much vacuum the PVC tubing will take and this will be important
in the internals of the microscope.

How can we identify the source of the problem? I'm proposing to put
foreline traps on both rotary pumps, but if a diff pump is the problem,
this won't solve it. I'm also proposing to take out the diff pumps and
check them, change the oil, etc. I've had a look through Will Bigelow's
Vacuum Methods book (very useful!), and it's a bit like reading an index of
diseases and their symptoms - you think you have a whole variety of them!!
- I can see several incidents and problems that could have affected a
number of the vacuum components.


Thanks for any and all advice!
cheers,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au





From daemon Mon May 6 18:20:45 2002



From: Ron Anderson :      microtoday-at-attglobal.net (by way of
Date: Mon, 6 May 2002 18:11:03 -0500
Subject: MICROSCOPY TODAY May/June Issue: Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

Listed below is the table of contents of the May/June issue of
MICROSCOPY TODAY (MT) which is the newest publication of
the Microscopy Society of America (MSA), the host of the
Microscopy Listserver.

As a service to the Microscopy Community and begining with the
May/June issue of MT the table of contents of MT, will be routinely
submitted to the Listserver.

Issues of Microscopy-Today are free, thanks to our advertisers, for any
individuals who receive delivery of their copies in the USA.
Non-USA subscribers who are also members of MSA will also receive
free subscriptions. Non-USA, non-MSA members, who wish to receive
copies of MT will find subscription rates on the MT web site
(http://www.microscopy-today.com). In the transition from the
previous publisher (Don Grimes) into an MSA publication, we
have found there were a number of non-USA, paid subscribers, who are
also MSA members. We plan to credit these folks appropriately.

The entire MSA membership has been added to the MT subscriber list and
as many duplicates as possible have been removed. We are left with
potential duplicates for the case where two records have the same name
but different addresses. If you have moved and did not change your
address or if you are receiving two copies, please let me know
by Email at: microtoday-at-attglobal.net.

I plan to close the subscription list and generate address labels for
the May/June issue on May 8th. People who subscribe after that date
will begin to receive their issues in July.

All subscriptions and address changes should go to
http://www.microscopy-today.com directly or via "publications of the
society" in http://www.msa.microscopy.com .

Ron Anderson, Editor
Email: microtoday-at-attglobal.net
________________________________________________


MICROSCOPY TODAY, May/June 2002
__________________________
Index of Articles
__________________________


Food Under The Microscope
Stephen Carmichael, Mayo Clinic

Some Thoughts On Vibrations In EM Laboratories
Anthony J. Garratt-Reed, MIT

The Emergence of Aberration Correctors for Electron Lenses
John Silcox, Cornell University

Fast OIM
D. J. Dingley*, S. Wright and M. Nowell TSL
(a subsidiary of EDAX)

Fitting a Student Microscope with a Consumer Digital Camera
Theodore M. Clarke, Metallurgical F. A. Consultant

Microscopy in the Real World: A Manufacturer's Perspective
Michael M. Kersker, JEOL USA, Inc.

All That Glitters is not Gold: Approaches to Labeling for TEM
R.M. Albrecht and D.A. Meyer, University of Wisconsin

Low Voltage Scanning Electron Microscopy and Jack Ramsey's principle
Oliver C. Wells, IBM Research Division

More on the Calibration of TEMs
J. P. McCaffrey, N.R.C. and R. Beanland,
Bookham Technology PLC

Lateral Resolution in Scanning Force Microscopy
Brian A. Todd and Steven J. Eppell
Case Western Reserve University

Utilizing Original TEM Negatives and Micrographs For Teaching in the
Digital Domain
José A. Mascorro, Tulane University

Plunge-Freezing into Slush Nitrogen
Philip Oshel, University of Wisconsin

Downloadable Photoshop Convolution Plug-In
John Russ, North Carolina State University

A Home-made Antifade Medium for Fluorescent Dyes
Tim Plummer, Mayo Clinic


From daemon Mon May 6 18:32:13 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Tue, 7 May 2002 09:29:11 +1000
Subject: Re: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for all of your replies!

A few people suggested checking the water cooling system - we had to
replace the chiller last year, and we're replacing the water lines now at
the same time as we replace the vacuum lines in this overhaul - there are a
couple of very slow water leaks and some of the old rubber water lines are
very stiff. Our water tends to be on the cool side rather than too hot -
never more than 20C, though we did have a period of about a week or two
after the new chiller went in when the water got up to 24-25C. And all the
EMs were on tap water for about 6 months while we waited for the new
chiller....... But the oil problem started long before this.

The amazing thing about this TEM is that the image quality is pretty good.
However, some oil (or something) has definitely got into the column because
during an EM maintenance training session last year, we took the top off
the column and cleaned the upper chamber and gun - the inside of the
chamber was brown! However, since this was its first clean in at least 12
years, perhaps this isn't surprising. The TEM itself is 25 years old (so
the diff pump oil is also 25 years old) and has been in this building for
12 years. It may be that the move to this building caused a minor leak
somewhere in the system.

The guys have just started taking the diff pumps out, and we can see oil
leaking out of the solenoids on the vacuum system at the back of the
column..... We're all going to have fun, I can see!!!

Once it's clean and put back together, we'll follow Allen Sampson's
suggestion re. checking the cross-over vacuum setting.

cheers,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au




From daemon Mon May 6 23:07:56 2002



From: Xianglin Li :      Xianglin_Li-at-student.uml.edu
Date: Mon, 06 May 2002 23:59:48 -0400
Subject: About the Z value of AFM image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear friends,

I met a problem about the AFM images. As you know, although there is
no real meaning of 3-D image in AFM, it is a good format to show the
AFM result.
But the thing is: How to make two images have the same Z value?

My AFM system is the ThermoMicroscope. The software I use is Proscan.
Could somebody help me to find out how to get the same Z value for
different images out of my system? Or could you introduce another
reasonable software for me?

Looking forward for your help!

Yours, Xianglin Li

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Tue May 7 00:26:42 2002



From: Allen Sampson :      ars-at-sem.com
Date: Tue, 7 May 2002 00:19:10 -0700
Subject: RE: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How often do you pump down the camera?

As far as the PVC tubing, I have rarely seen cases where the proper
installation of roughing pump tubing of any kind induces a significant
vibration problem. Far more common are the building vibrations induced
through the structural members of the EM. However, I did qualify that as a
proper installation of the tubing. Case in point - one manufacturer's
service rep who replaced the tubing from the optics table to the vibration
isolator (the lead or concrete filled block usually used to dampen
vibrations). He added around 8 feet of extra tubing that he left coiled
on the floor claiming that it was known that that length of tubing was
required to reduce vibrations. Needless to say, when the excess was
removed the customer was greatly relieved that his FESEM actually could
image above 20KX.

Old DP oil can be a problem and the original poster has admitted to an
inordinate amount of time between changes. I assumed changes around 5
years and am culpable once again for the assumption that the service
organizations are doing their business properly and an apparently aware
operator would be apprised of the timing of good maintenance. Mea culpa.

Santovac 5 another one of my favorites. Anyone who knows me knows that I
am reluctant to endorse any particular product or service, but you've hit
two of my favorites in one posting and I can't help it.

You also bring up another pet peeve of mine - improperly placed foreline
traps. A foreline trap is a device that is inserted between a mechanical
pump and the instrument that is designed to condense oil vapors
backstreaming from the mechanical pump. Ideally, they should be placed at
least a few inches from the inlet of the pump, to provide thermal isolation
from the pump. A trap at room temperature will do a better job than one at
an elevated temperature caused by close contact with a constantly operated
pump.

The trap should be placed vertically so that the condensed oils can be
drawn back into the pump by gravity. Nothing worse than being called in to
fix an instrument that won't pump down only to find the foreline trap
laying horizontal on the floor and it and the vacuum hose flooded with
collected oil.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Monday, May 06, 2002 2:42 PM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
wrote:
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
}
} } Date: Mon, 06 May 2002 14:39:09 -0700
} } To: "ars-at-sem.com" {ars-at-sem.com}
} } From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} } Subject: RE: cleaning vacuum system
} }
} } The only things you have to keep in mind: PVC tubes are less flexible
and
} } may 'transfer' vibration from rotary pump to the microscope's column.
For
} } this reason, I love to use PVC on vacuum evaporators nor the
microscopes.
} }
} } As for oil in the column - to me it looks like DP problem and water
} } waffler, which is situate over DP. The reason I think so, that usually
we
} } do not pump columns down by RP very often. It happening on my
microscope
} } a few times over 15 years, I believe. So, it mean, that RP do not touch
} } column frequently (except terrible downstream if it happening). I do
} } believe that 100C/CX DPs have water wafflers, check does water go
} } through. Another things: if DP oil is old (or overheated over some
period
} } of time) it may be deteriorated and boiling point may decreased (shorter
} } molecules), so some low-weight components may not condensate effectively
} } and will migrate into the column. I would suggest to check DP oil and
} } replace it on Santovak-5 like type (and forget for 5+ years). If you
} } decided to replace DP oil, I would suggest, you install oil traps on RPs
} } and new tubings as well to protect fresh oil (you never know...). Good
} } luck! Sergey
} }
} } At 05:04 AM 5/6/02 -0700, you wrote:
}
} } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
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}
} } -----------------------------------------------------------------------.
} } }
} } }
} } } Yes, a changeover to the clear PVC hosing is appropriate - I love the
} } } stuff. The problem you are experiencing is probably more a consequence
of
} } } the calibration of the vacuum system than anything else.
} } }
} } } Anyone who has been here for awhile has probably seen postings from me
on
} } } the vacuum system calibrations of EMs regarding the cross-over points
of
} } } diffusion pumped systems. Too often, design engineered or field
} } } engineering solutions result in a cross-over vacuum level from roughing
to
} } } diffusion pumps at too high a pressure level. When a diffusion pump is
} } } opened to a pressure level too high, it can stall which results in the
} } } breakdown of the normal laminar flow of oil vapor to a chaotic flow
which
} } } results in the influx of oil vapor into the sample chamber.
} } }
} } } Your first step should be to determine the actual chamber pressure at
which
} } } the cross-over from roughing to diffusion pump occurs. At the least,
you
} } } can get a qualitative determination from the action of the chamber
vacuum
} } } when the diffusion pump kicks in. At that point, the vacuum level
should
} } } start a rapid increase to the ultimate vacuum. If, instead, the vacuum
} } } level declines or holds steady for a few seconds, the cross-over point
is
} } } too high and the diffusion pump is stalling. Cross-over should
normally
} } } occur at 70 - 100 microns, most diffusion pumps will react well in this
} } } area. I generally tend to set the cross-over at 70 microns - it will
take
} } } longer for the roughing time but when the diffusion pump kicks in it
will
} } } rapidly pull down to the ultimate vacuum and the sample chamber will
remain
} } } cleaner.
} } }
} } } Field engineers don't normally carry independant vacuum measuring gear.
} } } Instead, they depend on known timing characteristics of known good
} } } systems. If your system doesn't match the characteristics expected,
the
} } } calibration will be off. The only offset to this is the use of a leak
back
} } } test, where the actual leak rate of the system can be tested. If the
leak
} } } back can be verified as within the manufacturer's specs, then the
} } } calibration can be applied and expected to achieve specs.
} } }
} } } If, however, there is no absolute measurement made of either actual
vacuum
} } } levels or actual leak rates, then no assumptions can possibly be made
of
} } } the vacuum levels achieved. Turbo-pumping systems actually make this
} } } determination of vacuum levels more problematic as they are seldom
capable
} } } of closing a valve on the sample chamber to allow the determination of
its
} } } leak rate, making the use of accurate vacuum level measuring equipment
} } } neccessary to determining the the calibration of system levels.
} } }
} } }
} } }
} } } Allen R. Sampson
} } } Advanced Research Systems
} } } 317 North 4th. Street
} } } St. Charles, Illinois 60174
} } }
} } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
} } }
} } }
} } } On Sunday, May 05, 2002 11:50 PM, Rosemary White
} } } [SMTP:rosemary.white-at-csiro.au] wrote:
} } } }
------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
-----------------------------------------------------------------------.
} } } }
} } } }
} } } } Dear all,
} } } }
} } } } We have a Jeol 100CX TEM that, I am told, has developed the
following
} } } } vacuum problem over the last 5-7 years. The problem, whatever its
cause,
} } } } means that the viewing port into the observation chamber has to be
} } } removed
} } } } once a month or so to clean off the buildup of oil on the inside. I
am
} } } } amazed that this was considered Ok for the microscope........ So,
my
} } } } proposal is to replace all the rubber vacuum lines, and clean out,
as
} } } best
} } } } as possible, the remaining components - metal hoses, etc. We're not
sure
} } } } of the cause of this problem, whether it's oil from the rotary pumps
or
} } } } from one of the diffusion pumps. Oh, and we need to clean, or at
least
} } } } check, the column as well, of course......
} } } }
} } } } My questions are:
} } } } Can we replace the original rubber tubing with wire-reinforced clear
PVC
} } } } tubing (at least then we'd see any oil buildup in future)? It's a
little
} } } } unclear how much vacuum the PVC tubing will take and this will be
} } } important
} } } } in the internals of the microscope.
} } } }
} } } } How can we identify the source of the problem? I'm proposing to put
} } } } foreline traps on both rotary pumps, but if a diff pump is the
problem,
} } } } this won't solve it. I'm also proposing to take out the diff pumps
and
} } } } check them, change the oil, etc. I've had a look through Will
Bigelow's
} } } } Vacuum Methods book (very useful!), and it's a bit like reading an
index
} } } of
} } } } diseases and their symptoms - you think you have a whole variety of
} } } them!!
} } } } - I can see several incidents and problems that could have affected
a
} } } } number of the vacuum components.
} } } }
} } } }
} } } } Thanks for any and all advice!
} } } } cheers,
} } } } Rosemary
} } } }
} } } } Rosemary White
} } } } Microscopy Centre
} } } } CSIRO Plant Industry
} } } } GPO Box 1600
} } } } Canberra, ACT 2601
} } } } Australia
} } } }
} } } } 61- 2 6246 5475 or
} } } } 61- 0402 835 973
} } } } rosemary.white-at-csiro.au
} } } }
} } } }
} } } }
} } } }
} } } }
}
}
}
}



From daemon Tue May 7 00:40:39 2002



From: Allen Sampson :      ars-at-sem.com
Date: Tue, 7 May 2002 00:34:33 -0700
Subject: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rosemary, you've got a lot of 'xplaining to do.

Has this instrument had any service at all in all those years? These
instruments can be remarkable tolerant, especially if the proper supplies
are used such as a DP oil that will form a non-conductive film. However,
you have two choices - either make column cleanliness a regular affair
where small amounts of contamination are removed at regular intervals, or
make column cleanliness a rare but substantial affair where it is left
alone until a problem develops and a major cleaning is required.

I have some sympathy for the task you now have, but take solace in the fact
that you brought it upon yourself. What you have is one of the most
sensitive and sophisticated scientific instruments ever made. Treat it in
the future as the fine instrument it is and you will have many more years
of happy service from it.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Monday, May 06, 2002 4:29 PM, Rosemary White
[SMTP:rosemary.white-at-csiro.au] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Thanks for all of your replies!
}
} A few people suggested checking the water cooling system - we had to
} replace the chiller last year, and we're replacing the water lines now at
} the same time as we replace the vacuum lines in this overhaul - there are
a
} couple of very slow water leaks and some of the old rubber water lines
are
} very stiff. Our water tends to be on the cool side rather than too hot -
} never more than 20C, though we did have a period of about a week or two
} after the new chiller went in when the water got up to 24-25C. And all
the
} EMs were on tap water for about 6 months while we waited for the new
} chiller....... But the oil problem started long before this.
}
} The amazing thing about this TEM is that the image quality is pretty
good.
} However, some oil (or something) has definitely got into the column
because
} during an EM maintenance training session last year, we took the top off
} the column and cleaned the upper chamber and gun - the inside of the
} chamber was brown! However, since this was its first clean in at least
12
} years, perhaps this isn't surprising. The TEM itself is 25 years old (so
} the diff pump oil is also 25 years old) and has been in this building for
} 12 years. It may be that the move to this building caused a minor leak
} somewhere in the system.
}
} The guys have just started taking the diff pumps out, and we can see oil
} leaking out of the solenoids on the vacuum system at the back of the
} column..... We're all going to have fun, I can see!!!
}
} Once it's clean and put back together, we'll follow Allen Sampson's
} suggestion re. checking the cross-over vacuum setting.
}
} cheers,
} Rosemary
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} 61- 2 6246 5475 or
} 61- 0402 835 973
} rosemary.white-at-csiro.au
}
}
}
}
}



From daemon Tue May 7 01:47:19 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Tue, 7 May 2002 16:42:30 +1000
Subject: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Allen,

Yes, well this is the state the instrument was in when I arrived here 2
years ago, but was told "it's OK, it works fine, don't worry about the oil
on the viewing window". As the new kid on the block and with no
experience in EM maintenance, I found it hard to argue for this full
overhaul. And yes, you could get OK images from it, but I still worried
about the oil, so finally we are doing something about it.

The diff pumps are out now, and if any of you have looked at Fig. 5.15 in
Wilbur Bigelow's Vacuum Methods book you'll have an idea what their innards
looked like. The top pump had a little oil left in it, with big chunks of
black goop in - and it looks like some of this junk and oil has gone
further up the vacuum system. The hot parts had brown to black carbonised
oil burnt firmly onto the surface. The innards of the bottom diff pump
were coated in what looked like blackstrap molasses - what's left of the
original oil, I guess. There was less carbonisation than in the top pump.

So, we're in for a long session, by the looks.......

Thanks again to everyone for suggestions and comments.

cheers,
Rosemary

} Rosemary, you've got a lot of 'xplaining to do.
}
} Has this instrument had any service at all in all those years? These
} instruments can be remarkable tolerant, especially if the proper supplies
} are used such as a DP oil that will form a non-conductive film. However,
} you have two choices - either make column cleanliness a regular affair
} where small amounts of contamination are removed at regular intervals, or
} make column cleanliness a rare but substantial affair where it is left
} alone until a problem develops and a major cleaning is required.
}
} I have some sympathy for the task you now have, but take solace in the fact
} that you brought it upon yourself. What you have is one of the most
} sensitive and sophisticated scientific instruments ever made. Treat it in
} the future as the fine instrument it is and you will have many more years
} of happy service from it.
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au




From daemon Tue May 7 02:46:30 2002



From: Allen Sampson :      ars-at-sem.com
Date: Tue, 7 May 2002 02:40:00 -0700
Subject: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Funny stuff, DP oil. Over time and use, it seems to both crack, break down
into lower molecular weight products, and polymerize into larger molecular
weight fractions. The polymerization generally occurs on the metallic
surfaces of the pump and can only be removed by vigorous abrasive
techniques (metal polish and a lot of elbow grease or sandblasting with
glass beads - I haven't tried starch or CO2, but they may work). The gooey
mess can normally be removed with a good detergent (Dawn dishwashing soap)
or tri-chlor (not generally available anymore in the US). Mechanical
buffing wheels can be helpful but be aware that some pump parts may be made
of aluminum and will suffer material thinning if such means are used.

Sorry you inherited this mess. But it is a good guide to future
maintenance requirements.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Monday, May 06, 2002 11:43 PM, Rosemary White
[SMTP:rosemary.white-at-csiro.au] wrote:
} Dear Allen,
}
} Yes, well this is the state the instrument was in when I arrived here 2
} years ago, but was told "it's OK, it works fine, don't worry about the
oil
} on the viewing window". As the new kid on the block and with no
} experience in EM maintenance, I found it hard to argue for this full
} overhaul. And yes, you could get OK images from it, but I still worried
} about the oil, so finally we are doing something about it.
}
} The diff pumps are out now, and if any of you have looked at Fig. 5.15 in
} Wilbur Bigelow's Vacuum Methods book you'll have an idea what their
innards
} looked like. The top pump had a little oil left in it, with big chunks
of
} black goop in - and it looks like some of this junk and oil has gone
} further up the vacuum system. The hot parts had brown to black
carbonised
} oil burnt firmly onto the surface. The innards of the bottom diff pump
} were coated in what looked like blackstrap molasses - what's left of the
} original oil, I guess. There was less carbonisation than in the top
pump.
}
} So, we're in for a long session, by the looks.......
}
} Thanks again to everyone for suggestions and comments.
}
} cheers,
} Rosemary
}
} } Rosemary, you've got a lot of 'xplaining to do.
} }
} } Has this instrument had any service at all in all those years? These
} } instruments can be remarkable tolerant, especially if the proper
supplies
} } are used such as a DP oil that will form a non-conductive film.
However,
} } you have two choices - either make column cleanliness a regular affair
} } where small amounts of contamination are removed at regular intervals,
or
} } make column cleanliness a rare but substantial affair where it is left
} } alone until a problem develops and a major cleaning is required.
} }
} } I have some sympathy for the task you now have, but take solace in the
fact
} } that you brought it upon yourself. What you have is one of the most
} } sensitive and sophisticated scientific instruments ever made. Treat it
in
} } the future as the fine instrument it is and you will have many more
years
} } of happy service from it.
} }
} } Allen R. Sampson
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} }
} } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} 61- 2 6246 5475 or
} 61- 0402 835 973
} rosemary.white-at-csiro.au
}
}
}
}



From daemon Tue May 7 02:50:20 2002



From: Dr Adam Papworth :      ajp5-at-liverpool.ac.uk
Date: Tue, 07 May 2002 08:47:24 +0100
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Unsubscribe

--
Dr Adam Papworth,
Senior Experimental Officer,
Department of Engineering,
The University of Liverpool,
Liverpool,
L69 3GH, UK.

Phone
(Work) 0151 794 4672
(Mobile) 0151 794 7587
07970 24 7587
(Home) 0151 283 8596
(FAX) 0151 794 4675
e-mail ajp5-at-liv.ac.uk




From daemon Tue May 7 03:15:23 2002



From: Reinhard Windoffer :      windoff-at-mail.uni-mainz.de
Date: Tue, 07 May 2002 10:03:43 +0200
Subject: hcRED

Contents Retrieved from Microscopy Listserver Archives
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Hello,
Clontech is offering a new red fluorescent Protein hcRed.
Has someone made any experience using this protein.
Of special interest is the question of oligomerizatin or aggregation in
living cells since the older red fluorescent proteins have this
tendency.

greetings
Reinhard


--
--------------------------------------------------------------------
Dr. Reinhard Windoffer phone ++49-(0)6131 39 23720
Universitaet Mainz fax ++49-(0)6131 39 23719
Anatomisches Institut internet windoff-at-mail.uni-mainz.de
Becherweg 13 www.uni-mainz.de/~windoff
55099 Mainz
--------------------------------------------------------------------




From daemon Tue May 7 07:38:49 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 7 May 2002 08:34:05 -0400
Subject: About the Z value of AFM image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Xianglin;

I am, and I would imagine others, are a bit confused as to what you are
using the AFM for? It sounds as if you are doing a thermal map over a
surface and not z-height measurements. If you are doing thermal
measurements are you attempting to combine them with z-height on a surface
as overlapping images?

Please elaborate and I'm sure someone can help.

Regards,
Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Xianglin Li [mailto:Xianglin_Li-at-student.uml.edu]
Sent: Tuesday, May 07, 2002 12:00 AM
To: Microscopy-at-sparc5.microscopy.com


Dear friends,

I met a problem about the AFM images. As you know, although there is
no real meaning of 3-D image in AFM, it is a good format to show the
AFM result.
But the thing is: How to make two images have the same Z value?

My AFM system is the ThermoMicroscope. The software I use is Proscan.
Could somebody help me to find out how to get the same Z value for
different images out of my system? Or could you introduce another
reasonable software for me?

Looking forward for your help!

Yours, Xianglin Li

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Tue May 7 08:29:48 2002



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Tue, 7 May 2002 09:23:01 -0400
Subject: RE:RONTEC USA Inc., Correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,
I had a slight memory lapse regarding Audi so I spent 5 minutes to refresh
my memory. Here is a quote from The Center for Auto Safety:
"With nearly 1800 reported unintended acceleration incidents in the 1980s,
Audi became synonymous with the term "sudden acceleration." Despite several
deaths, dozens of injuries, five related recalls and a Swedish defense
agency study showing cruise control malfunctions could cause sudden
acceleration, Audi claimed that "pedal misapplication" caused the incidents.
Audi lost 80% of its market share because it chose to blame its customers
for the problem."
http://www.autosafety.org/autodefects/AUDI.htm

If memory serves me correctly one of the accidents actually occurred in a
New Jersey emissions inspection station! Ouch that's likely to put a dent in
your corporate image. Seems to me the free market did its job. As Sergey
Ryazantsev mentioned in his reply, it is unlikely that a single negative
posting will damage a credible companies reputation. It is usually a very
simple matter to verify and correct misinformation posted to this site.
That being said, I further agree with Sergey that the list has rules
governing participation that should be observed and enforced.


} John A. Robson
} _____________________________________________________
}
} Boehringer Ingelheim Pharmaceuticals, Inc.
} Research and Development
} 900 Ridgebury Road / P. O. Box 368
} Ridgefield, CT 06877-0368
}
} phone: 203.798.5640
} fax: 203.798.5698
} email: jrobson-at-rdg.boehringer-ingelheim.com
}
}
}
}
} -----Original Message-----
} From: Mike Bode [SMTP:mb-at-Soft-Imaging.com]
} Sent: Friday, May 03, 2002 3:05 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE:RONTEC USA Inc., Correction
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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}
}
} Unfortunately that is the case. Someone posts damaging material on this
} (or
} any other) list, it get's sent around to some people a few times, and
} suddenly it is "the official opinion of the microscopy specialists". I am
} sure that can do substantial damage.
}
} And it's not limited to the web or list servers. Audi (the German car
} manufacturer) had a good business going in the US until someone decided to
} start the engine and put it in forward (or backwards, I don't remember)
} and
} run the car into a wall. There was a flaw, that allowed users to put the
} car
} in gear without stepping on the brakes. Although that definitely was a
} flaw,
} it was blown out of proportion and almost killed the Audi business in the
} US. It took them 10 years to get their reputation back, even though I
} would
} not consider this as dangerous as the tire problem that Firestone/Ford was
} experiencing last year.
}
} Bottom line: Everybody should be careful and check their sources when
} posting potentially dmaging material on the listserver.
}
} But that's just my 2 cents as a vendor.
}
} mike
}
} } } } } } } } } } } WE HAVE MOVED { { { { { { { { {
} please make a note of the new address below
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Friday, May 03, 2002 6:40 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: RONTEC USA Inc., Correction
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} It's strange to see that one anonymous message could make so much damage
} to
} the company. It seems to me that reputation of the company depends more
} on
} the quality of the products/service etc nor on the one anonymous
} opinion. From another hand, I think, this ListServer mostly is a place
} for
} 'customers' to exchange not only scientific ideas but to help each other
} to
} survive in this 'capitalistic' world. Information about wrongdoing may
} help others do not make similar mistake or spend money on bad quality
} product/service. This is sort of our 'protection' against bad quality
} service/products providers. Again, single decision even published here
} could not damage the reputation of the good company with long well
} established record of the service to EM community. I know, there are
} places on the Internet where you could leave your opinion about some
} particular business/company - it's perfectly legal and many institutions
} used that lists for decision making. This discussion is more about ethics
}
} - we all agree that we have rights openly speak here and others have the
} rights to know who is speaking.
}
} Sergey spoke.
}
} At 02:15 AM 5/3/02, you wrote:
} } ------------------------------------------------------------------------
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} } {http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} http://www.m
} sa
} .microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } To the Microscopy & Microanalysis community
} }
} } Ref.: Anonymous Message posted April 30,
} } Subj: RÖNTEC USA Closes Down Operation
} }
} } Ladies and Gentlemen:
} }
} } Without any doubt, the Microscopy ListServer is a great facility for the
} } Microscopy&Microanalysis community to distribute and exchange useful
} } information in a very efficient way and everyone involved in initiation
} } and maintenance of this forum highly deserves our community's credit.
} }
} } However, it appears that at the same time this platform is a quite
} } powerful tool for anyone with hostile and bad enough intents to defame
} and
} } defile disliked entities. This is what we had to learn when the
} } unwarranted message regarding an alleged close down of RÖNTEC USA, Inc.
} } was spread. It also appears that there is no way to avoid such unfounded,
}
} } false and misleading information to be posted at the ListServer other
} than
} } the commitment of all users to the "General Ground Rules on using the
} } Microscopy Listserver/Mailreflector" (see msa website), clearly a code of
}
} } honor. Whatever individual or organization posted this message in their
} } malicious attempt of damaging our company and good reputation violated
} all
} } those general ground rules. May everyone draw his/her own conclusions
} } concerning the nature of the information and the integrity of the sender.
} }
} } We may be allowed to correct the above-mentioned statements about RÖNTEC
} } USA, Inc. as follows: 1. There are no plans whatsoever to cease the
} } business of RÖNTEC USA, Inc.
} } 2. The RÖNTEC Group of companies - like so many others - has not
} } remained unaffected by the overall downturn we saw in the economy during
} } the year 2001. The situation within our group forced us to implement a
} } restructuring program in order to reduce operational costs (as was the
} } case throughout the industry - we believe we are in good company here).
} } This program was comprising all members of our group, including RONTEC
} } USA. The goal was not to cease business, but exactly the opposite - to
} } ensure its continuation, in the best interest of our customers.
} } 3. Our joint efforts have been successful. The fact is RÖNTEC USA,
} } Inc. has had its best sales year since the company's founding in 1999
} } nearly doubling its revenue over the previous year. Why, after having
} } worked very hard indeed to get things going and after having eventually
} } accomplished that stage, should we consider to discontinue the business?
} } Apparently there exists someone or some company who is attempting to
} } damage our reputation. Be assured that RÖNTEC USA is in a better position
}
} } than ever before and will continue to grow and continue to support its
} } customers.
} } 4. We have never provided misleading information on the equipment we
}
} } are offering. How long would we survive in this business if we were to
} } make promises we can't keep? Due to our companies policy of verifying any
}
} } information before disclosing it to the public we earned ourselves a
} } reputation as a reliable business partner.5. RÖNTEC USA, Inc. does
} } have an SEM for sale, however this has absolutely no bearing on the
} } viability of the company.
} }
} }
} } Whoever has any questions or the need for more information on this
} subject
} } may please contact us directly, by phone or otherwise, in the US or at
} our
} } German headquarters. We at RÖNTEC take great pride in our products and
} } services and we have nothing to hide - in contrast to the sender of that
} } unfounded message, who chose not to disclose a name.
} } In the future may our community be spared such unwarranted assaults.
} }
} }
} } Thomas Schuelein Paul Smith
} } President & CEO President & CEO
} } RÖNTEC Holding AG RÖNTEC USA, Inc.
} }
} }
} } {/blockquote} {/x-html}
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}



From daemon Tue May 7 08:38:34 2002



From: Steven L. Tripp :      tripp-at-purdue.edu
Date: Tue, 7 May 2002 12:14:14 -0500
Subject: EM of magnetic particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-----Ursprungliche Nachricht-----
Von: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Gesendet: Tuesday, May 07, 2002 3:03 PM
An: NewSub-at-sparc5.microscopy.com
Betreff: Welcome to the Microscopy Listserver


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


*********************************************
This Email contains Important Information about
the Microscopy Listserver. Please read it all then
SAVE a copy for future reference. - Nestor
****************************************
To: NewSub-at-MSA.Microscopy.Com
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}


Hi Peter:

I am very sorry to make you and other friends confused with my
question.

Actually, I am just doing the regular AFM, that is, using AFM to get
the topography information. It is the z height what you mentioned.

I want to put some images in my presentation. While, as you know, 3-D
works better than 2-D if you just want to "show" them. The problem is:
different 3-D images have their own Z range. If I want to compare
them, I'd better use the same Z range for every image, so that when
people look at them, they will have some idea immediately.

This is what I want to do: Keep same Z range for different sample's
image. But I failed when I tried to reach it by only changing the Z
magnification and offset.

But for lots of papers, which have AFM images included, their images
have the constant Z range for all the images. So I just wonder whether
some of you did this for your own images, and whether you could help
me out about this.

Thank you very much!

Sincerely, Xianglin Li


Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411


Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411


----- Original Message -----
} From: Peter Tomic {PTomic-at-Anadigics.com}


I am working with 27nm cobalt particles that are weakly ferromagnetic (MS =
95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
am thinking of also using a JEOL 840 SEM to analyze the aggregated
structures formed once the particles are dried onto a substrate. The
substrates I am using are carbon grids for the TEM and I am planning to use
silicon wafers for SEM. I am unsure of the adhesion of the particles to the
substrates, although suspect adhesion to the carbon grid will be better than
to the silicon (the particles are coated with an organic surfactant). I
would prefer to obtain the SEM images without coating the sample. What is
the possibility that these particles will be picked up by the lenses in the
EM? Are there other considerations I should keep in mind while planning
these experiments?


Thank you,

Steve Tripp

Dept. of Chemistry
Purdue University
West Lafayette, IN 47907




From daemon Tue May 7 15:27:50 2002



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Tue, 7 May 2002 16:19:31 -0400
Subject: RE: EM of magnetic particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the early days of materials ultramicrotomy at my laboratory, we plastered
more than a few sections of steel onto the polepiece of our EM400T as soon
as the polepiece was activated in going from low mag to regular mode. One
minute they were there, then---poof! However in an old warhorse like that,
there was no noticeable effect on the TEM's performance. (Our
superconscientious tech did glare rather strongly just at the thought of
such an indiscretion, mind).

So I wouldn't worry too much. We got tired of losing metal thin sections
that were dried onto grids in any event, and found that collecting them on
the film side of a coated grid resulted in more than enough 'stick' to hold
them there. Of course, these sections had pretty huge aspect ratios
(thickness/width of several hundred) compared to a particle, but I think
your low mass should help out there.

Good luck.

Tom

Dr. Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada K1A 0G1

ph. 613-992-2310
FAX 613-992-8735

email: malis-at-nrcan.gc.ca
(currently on assignment as Science Advisor to DG/MTB, can be reached at
613-995-7358, same email)


} ----------
} From: Steven L. Tripp
} Sent: Tuesday, May 07, 2002 1:14 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: EM of magnetic particles
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am working with 27nm cobalt particles that are weakly ferromagnetic (MS
} =
} 95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
} and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
} am thinking of also using a JEOL 840 SEM to analyze the aggregated
} structures formed once the particles are dried onto a substrate. The
} substrates I am using are carbon grids for the TEM and I am planning to
} use
} silicon wafers for SEM. I am unsure of the adhesion of the particles to
} the
} substrates, although suspect adhesion to the carbon grid will be better
} than
} to the silicon (the particles are coated with an organic surfactant). I
} would prefer to obtain the SEM images without coating the sample. What is
} the possibility that these particles will be picked up by the lenses in
} the
} EM? Are there other considerations I should keep in mind while planning
} these experiments?
}
}
} Thank you,
}
} Steve Tripp
}
} Dept. of Chemistry
} Purdue University
} West Lafayette, IN 47907
}
}
}


From daemon Tue May 7 16:15:13 2002



From: Kathy Napolitano :      kathy-at-napolitano.com
Date: Tue, 7 May 2002 13:52:46 -0700
Subject: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
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Yuck I've scrubbed DP's like that before, Sorry You inherited this mess but
they will be amazed at how good it works when you are done. yearly PM's are
well worth never having to do this again I'm sure you will agree by the time
you get it all put back together. Good Luck.

Kathy Napolitano

-----Original Message-----
} From: Rosemary White [mailto:rosemary.white-at-csiro.au]
Sent: Monday, May 06, 2002 11:43 PM
To: ars-at-sem.com
Cc: Microscopy-at-sparc5.microscopy.com


Dear Allen,

Yes, well this is the state the instrument was in when I arrived here 2
years ago, but was told "it's OK, it works fine, don't worry about the oil
on the viewing window". As the new kid on the block and with no
experience in EM maintenance, I found it hard to argue for this full
overhaul. And yes, you could get OK images from it, but I still worried
about the oil, so finally we are doing something about it.

The diff pumps are out now, and if any of you have looked at Fig. 5.15 in
Wilbur Bigelow's Vacuum Methods book you'll have an idea what their innards
looked like. The top pump had a little oil left in it, with big chunks of
black goop in - and it looks like some of this junk and oil has gone
further up the vacuum system. The hot parts had brown to black carbonised
oil burnt firmly onto the surface. The innards of the bottom diff pump
were coated in what looked like blackstrap molasses - what's left of the
original oil, I guess. There was less carbonisation than in the top pump.

So, we're in for a long session, by the looks.......

Thanks again to everyone for suggestions and comments.

cheers,
Rosemary

} Rosemary, you've got a lot of 'xplaining to do.
}
} Has this instrument had any service at all in all those years? These
} instruments can be remarkable tolerant, especially if the proper supplies
} are used such as a DP oil that will form a non-conductive film. However,
} you have two choices - either make column cleanliness a regular affair
} where small amounts of contamination are removed at regular intervals, or
} make column cleanliness a rare but substantial affair where it is left
} alone until a problem develops and a major cleaning is required.
}
} I have some sympathy for the task you now have, but take solace in the fact
} that you brought it upon yourself. What you have is one of the most
} sensitive and sophisticated scientific instruments ever made. Treat it in
} the future as the fine instrument it is and you will have many more years
} of happy service from it.
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au







From daemon Tue May 7 19:46:46 2002



From: Dmrelion-at-aol.com
Date: Tue, 7 May 2002 20:32:04 EDT
Subject: LM-illuminator for POS

Contents Retrieved from Microscopy Listserver Archives
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We are looking for a simple sub-stage illuminator for an old Olympus POS
vertical monocular microscope. Any suggestions would be appreciated.

Don Marshall

Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

781-275-4695 (Phone)
781-271-0252 (FAX)

dmrelion-at-aol.com

http://www.excitingelectrons.com


From daemon Tue May 7 21:25:58 2002



From: Mr.WILKES :      gimy-at-public.ayptt.ha.cn
Date: Wed,8 May 2002 10:20:05 +0800
Subject: OIL REFINING EQUIPMENT

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Dear Sir,

We are glad to send the introductory to you in order to enter into
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E-mail: chineseoilpress-at-hotmail.com





From daemon Wed May 8 02:32:28 2002



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Wed, 08 May 2002 09:23:09 +0200
Subject: Materials polishing

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for Microscopy-at-MSA.Microscopy.Com; Wed, 08 May 2002 09:23:20 +0200
Message-ID: {3CD8D25D.A48BB9E9-at-ru.ac.za}


Hi.
We're investigating various alternatives for the polishing of rocks
of various kinds. One of the suggestions is to go the Struers' MD-system
route, but as you can imagine this is the expensive option. Is there
anyone out there using this and how is it working for you? My particular
concerns are lif time of the discs and enbedding of foreign particles
(mineral frags that may chip off) into the polymer surface rendering
these unusable.
Thanks,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (try your luck!)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA




From daemon Wed May 8 02:51:21 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 8 May 2002 08:46:49 +0100 (GMT Daylight Time)
Subject: Re: EM of magnetic particles

Contents Retrieved from Microscopy Listserver Archives
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Hi Steven,

Common sense is (as always) most important. However, as
most common sense comes with hindsight I'll give a few
thoughts.

I'll assume that you are not thinking of applying fields
in-situ or looking for magnetic contrast but if you are
there are other considerations.

Don't forget that unless you have a low field pole piece
the specimen will be sitting in a field when you analyse
it. In the TEM this will be a high field and may negate
your ex-situ experiment.

It seems sensible to use a long working distance in the
SEM to reduce the field but I have no idea what a SEM field
profile looks like.

In the TEM switch off the objective lens before inserting
the specimen and slowly increase the strength to avoid
sudden field changes.

Even if using a low field pole piece don't forget the
return path of the lens field is through the outer column
and there will be a high field as the specimen is inserted
through the airlock.

Keep your beam current low to prevent the possibility of
particle charging adding to the force to free the particles
from the substrate.

Don't forget to tap the specimens to free any loose
particles before inserting them into the microscope.

Check particle dispersion under an optical microscope
first, large agglomerations are more likely to give
problems. (You may not see the particles but you will see
large agglomerations of particles.)

Good luck,
Ron


On Tue, 7 May 2002 12:14:14 -0500 "Steven L. Tripp"
{tripp-at-purdue.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am working with 27nm cobalt particles that are weakly ferromagnetic (MS =
} 95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
} and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
} am thinking of also using a JEOL 840 SEM to analyze the aggregated
} structures formed once the particles are dried onto a substrate. The
} substrates I am using are carbon grids for the TEM and I am planning to use
} silicon wafers for SEM. I am unsure of the adhesion of the particles to the
} substrates, although suspect adhesion to the carbon grid will be better than
} to the silicon (the particles are coated with an organic surfactant). I
} would prefer to obtain the SEM images without coating the sample. What is
} the possibility that these particles will be picked up by the lenses in the
} EM? Are there other considerations I should keep in mind while planning
} these experiments?
}
}
} Thank you,
}
} Steve Tripp
}
} Dept. of Chemistry
} Purdue University
} West Lafayette, IN 47907
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed May 8 04:59:54 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Wed, 8 May 2002 11:51:49 +0200
Subject: Line directions from TEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
What programs are people using these days for calculating the line direction
of dislocations and other linear features (and thus also habit planes of
planar features) from measurements made on conventional TEM diffraction
contrast images?

Several years ago I did this with Diffract (for Mac) as a PhD student, but
no error was included in the analysis, and Diffract was always prone to
unexpected crashes. Maybe this is done better in Desktop Microscopist (the
successor to Diffract) but I don't know myself.

Or maybe there's some other program to recommend?

I remember seeing a routine published by Fu-Rong Chen and Alex King for
Hexagonal Materials which calculated a best fit to available data. Did
anyone make such a thing into a program? I could probably do it with Excel,
but if somebody has already done it then it saves the work.

Well, I look forward to hearing from you.

Best wishes

Ian MacLaren
N.B. New address
FB Materialwissenschaft - FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162893
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Wed May 8 07:47:42 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 8 May 2002 08:44:08 -0400
Subject: EM of magnetic particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve;

Would there be a problem in elevating your samples above the Fermi
Temperature and thereby make them non-magnetic? Or would this change the
characteristics of your alloy? I am interested since I have had a similar
problem with an iron alloy but solved it by coating and imaging as you have
mentioned.

With respect to these particles on a Si wafer in SEM, you may try depositing
Si3n4 [silicon nitride] over the particles while on the wafer if you have
access to a deposition system such as a PECVD. This can be a very thin
film, just a few hundred angstroms, but it will lock these particles to the
surface and still allow imaging even if they are irregular surfaces.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Steven L. Tripp [mailto:tripp-at-purdue.edu]
Sent: Tuesday, May 07, 2002 1:14 PM
To: Microscopy-at-sparc5.microscopy.com


I am working with 27nm cobalt particles that are weakly ferromagnetic (MS =
95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
am thinking of also using a JEOL 840 SEM to analyze the aggregated
structures formed once the particles are dried onto a substrate. The
substrates I am using are carbon grids for the TEM and I am planning to use
silicon wafers for SEM. I am unsure of the adhesion of the particles to the
substrates, although suspect adhesion to the carbon grid will be better than
to the silicon (the particles are coated with an organic surfactant). I
would prefer to obtain the SEM images without coating the sample. What is
the possibility that these particles will be picked up by the lenses in the
EM? Are there other considerations I should keep in mind while planning
these experiments?


Thank you,

Steve Tripp

Dept. of Chemistry
Purdue University
West Lafayette, IN 47907




From daemon Wed May 8 08:57:26 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Wed, 8 May 2002 09:25:25 -0400
Subject: RE: cleaning vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


can anyone tell me where i might purchase uranyl formate in the US?


From daemon Wed May 8 08:57:27 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Wed, 8 May 2002 09:26:29 -0400
Subject: uranyl formate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


can anyone tell me where i might purchase uranyl formate in the US?


From daemon Wed May 8 09:41:16 2002



From: Poirier, Glenn :      glpoirie-at-nrcan.gc.ca
Date: Wed, 8 May 2002 10:34:16 -0400
Subject: Particle Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning to all.

I was wondering if the group had any wisdom to impart that could help me
with my particle analysis work. I'm trying to analyse stack effluent
collected on polycarbonate filters during an airborne survey. I'm using WDS
because the sample contain Pb and S. The particles range from less than one
to ~ 10 um. My problem is that most of the are charging and therefore the
beam is drifting off of them during the course of analysis. The samples
polycarbonate filtres are attached to Al stubs using double stick carbon and
heavily carbon coated while on a planetary rotating table. I'm using 15 kV
(I need to see Fe)and 10-20 nA current. I've had some success overscanning
the particles (i.e analysing with a raster that is the same size as the
particle) but some of the larger particles are multiphase and i would like
to use a smaller beam to determine their chemistry. Any tips or comments
from people with experience in this type of work would be gladly accepted.

Thanks

Glenn
Glenn Poirier
Microbeam Specialist

Mine and Mineral Science Laboratories (CANMET)
Rm. 213b, 555 Booth st.
Ottawa, On, K1A 0G1
TEL: (613) 947-9833
glpoirie-at-nrcan.gc.ca


From daemon Wed May 8 15:02:47 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 8 May 2002 15:58:32 -0400
Subject: EM of magnetic particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the below first paragraph below I mentioned "Fermi Temperature" and
should have said "Curie Temp."

My appologies.

Peter

-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Wednesday, May 08, 2002 8:44 AM
To: 'Steven L. Tripp'; Microscopy-at-sparc5.microscopy.com


Steve;

Would there be a problem in elevating your samples above the Fermi
Temperature and thereby make them non-magnetic? Or would this change the
characteristics of your alloy? I am interested since I have had a similar
problem with an iron alloy but solved it by coating and imaging as you have
mentioned.

With respect to these particles on a Si wafer in SEM, you may try depositing
Si3n4 [silicon nitride] over the particles while on the wafer if you have
access to a deposition system such as a PECVD. This can be a very thin
film, just a few hundred angstroms, but it will lock these particles to the
surface and still allow imaging even if they are irregular surfaces.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Steven L. Tripp [mailto:tripp-at-purdue.edu]
Sent: Tuesday, May 07, 2002 1:14 PM
To: Microscopy-at-sparc5.microscopy.com


I am working with 27nm cobalt particles that are weakly ferromagnetic (MS =
95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
am thinking of also using a JEOL 840 SEM to analyze the aggregated
structures formed once the particles are dried onto a substrate. The
substrates I am using are carbon grids for the TEM and I am planning to use
silicon wafers for SEM. I am unsure of the adhesion of the particles to the
substrates, although suspect adhesion to the carbon grid will be better than
to the silicon (the particles are coated with an organic surfactant). I
would prefer to obtain the SEM images without coating the sample. What is
the possibility that these particles will be picked up by the lenses in the
EM? Are there other considerations I should keep in mind while planning
these experiments?


Thank you,

Steve Tripp

Dept. of Chemistry
Purdue University
West Lafayette, IN 47907




From daemon Wed May 8 15:30:52 2002



From: Ladd Research :      sales-at-laddresearch.com
Date: Wed, 08 May 2002 16:24:58 -0400
Subject: Re: uranyl formate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We sold uranyl formate for many years but we discontinued it in 1995 and
have been unable to find a new source.

John Arnott
--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
FAX: 1-802-660-8859
e-mail: sales-at-laddresearch.com

Quality Since 1955


John Hoffpauir wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} can anyone tell me where i might purchase uranyl formate in the US?


From daemon Wed May 8 17:15:26 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 08 May 2002 18:05:11 -0500
Subject: Uranyl formate availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

John Hoffpauir wrote:
==================================================
can anyone tell me where i might purchase uranyl formate in the US?
==================================================
Try URL
http://www.2spi.com/catalog/chem/stain.shtml

The product is in stock.

Chuck

Disclaimer: SPI Supplies is a supplier of uranyl formate.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Wed May 8 20:07:14 2002



From: Stan Gelles :      sgelles-at-cctlabs.com
Date: Wed, 8 May 2002 19:51:07 -0500
Subject: Nikon Condenser Lens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We need to replace a Nikon Condenser Lens (MBL12100-AL1) from a Nikon Phase
Contrast Unit (1.25 59842) being used on a Nikon Labophot Microscope.
This microscope is being used for the phase contrast analysis of air
samples for fiber concentration.

We have been in touch with Nikon and one of their suppliers but so far
without too much success. Does anyone know of any other sources for this
lens or a replacement phase contrast unit?

Thanks .

Stanley H. Gelles
Principal Scientist
CC Technologies
6141 Avery Road
Columbus, OH 43220
614-761-1214
FAX 614761-1633
sgelles-at-cctlabs.com


From daemon Wed May 8 20:07:14 2002



From: alm958-at-lulu.it.northwestern.edu ()
Date: Wed, 8 May 2002 19:55:51 -0500
Subject: Ask-A-Microscopist: Imaging Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (alm958-at-lulu.it.northwestern.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
May 8, 2002 at 17:58:01
---------------------------------------------------------------------------

Email: alm958-at-lulu.it.northwestern.edu
Name: Tony Meier

Organization: Northwestern University

Education: Graduate College

Location: Evanston, IL ,USA

Question: Why are we getting a clear and focused image with our
infinity corrected objective attached directly to our vidicon camera,
without using a tube lens? I realize that we are not getting
anywhere near the 10x mag of the objective but I don't understand why
we are getting an image at all since we are not forming a real image
on our cameras sensor.

Thanks

---------------------------------------------------------------------------


From daemon Wed May 8 23:05:23 2002



From: Tina Schwach :      tschwach-at-mindspring.com
Date: Wed, 8 May 2002 23:04:01 -0500
Subject: In search of a Fluorescent Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking to purchase a fluorescent microscope ( I am partial to Olympus
and Leica but am open to other options). It needs to have oil (100X) and
phase contrast for LM, DIC would be nice too, and all three filter sets,
DAPI, FITC, and TRITC. A used one in good condition would be acceptable.

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112



From daemon Thu May 9 00:52:52 2002



From: charles j day :      wa5ekh-at-juno.com
Date: Wed, 8 May 2002 00:39:52 -0500
Subject: Subject: Negative Scanners-noncontact

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in the current negative-scanner technology, but I would
like to find a non-contact scanner. EM emulsions are very soft and very
high resolution, so since these negatives represent the basis for long
term archiving, I wouldn't want to sacrifice microscopic information by
placing it on a surface of glass (or any surface) with potentially
damaging particulate on the surface. Even projection holders for
Negatives have no glass that contact the emulsion.
So what is the current negative scanner technology?? Non-contact..?

Jeff Day/Texas
wa5ekh-at-juno.com


From daemon Thu May 9 05:49:33 2002



From: prof. Bruno Dore :      bruno.dore-at-unito.it
Date: Thu, 09 May 2002 12:30:38 +0200
Subject: LM, GMA resin, disposable blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


During several years I used Ralph Knifes to section Glycol Methacrylate
embedded material (2-3 micrometer) for LM.
Has anyone experience in sectioning with disposable blades?

Thanks for the courtesy
Bruno Dore




From daemon Thu May 9 10:20:50 2002



From: George Laing :      scisales-at-ngscorp.com
Date: Thu, 9 May 2002 11:12:20 -0400
Subject: RE: Subject: Negative Scanners-noncontact

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Many of our customers are using Microtek Artixscan 2500 and 1100 scanners
as well as their mechanical twins, the Agfa Duoscan T2500 and Hi-D. Agfa
has discontinued the Duoscan line but the Microteks are still available.
The 2500 has higher optical resolution, the 1100 has a wider dynamic range.
These scanners hold films in a glassless drawer much like a negative carrier
in an enlarger. Most are using the 4x5 opening to hold the 3.25 x 4 EM
negs.

More information is available at http://www.microtekusa.com/as2500.html for
the 2500, and
http://www.microtekusa.com/as1100.html for the 1100. We also have a PDF
available.


George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com



} I am interested in the current negative-scanner
} technology, but I would like to find a non-contact
} scanner.




From daemon Thu May 9 11:07:03 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Thu, 9 May 2002 12:00:52 -0400
Subject: LaB6 filament reconditioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I just wondered if anybody heard about LaB6 filament reconditioning and if
it's worth it.


Thanks,
Pavel
atcsem-at-earthlink.net



From daemon Thu May 9 11:56:40 2002



From: Jon Mulholland :      jwm-at-genome.stanford.edu
Date: Thu, 9 May 2002 09:49:17 -0700 (PDT)
Subject: Stanford position availible

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Life Science Research Assistant (LSRAI) (HR #001178). Range: 2P1.

DESCRIPTION: Life Sciences Research Assistant is sought for day to day
managing of the Cell Sciences Imaging Facility (CSIF). The CSIF is a
service center that specializes in the use of high technology fluorescent
microscopes (confocal, deconvolution, ratio imaging) in biomedical
research. LSRA duties include: training users in operation of microscopes,
providing ongoing technical supervision, performing routine
maintenance/cleaning and arranging for service of microscopes and
computers. Additionally, LSRA will provide some administrative support
such as assisting in setting up user accounts, updating user databases and
monthly billing routines.

All of CSIF microscopes are interfaced with computers for data collection
and analysis. Basic knowledge of operating systems (Windows, Mac, Unix ),
graphics programs (PhotoShop, Illustrator) and general computer usage
(transfer of files between platforms, file format differences) is
essential. LSRA should understand optical properties of fluorescence.
Preference will be given to applicants who have both basic microscopy and
computer experience, but a willingness to learn is key. Qualifications: BS
degree required, as well as good communication skills, patience, and the
ability to work independently. Good problem solving skills are essential.
This is a great opportunity learn state-of-the-art technology and be
exposed to current biomedical research.

Salary range $37,100 - 41,000, depending upon experience. There is no
relocation assistance; position is available immediately. Please send
resume directly to:

Jon Mulholland, Director
Cell Sciences Imaging Facility
Beckman Center B050
Stanford University School of Medicine
Stanford, CA 94305

650-725-4951 (f)



From daemon Thu May 9 12:33:00 2002



From: Smolko, Dan :      DSmolko-at-Nanogen.com
Date: Thu, 9 May 2002 10:21:12 -0700
Subject: SEM - 2D to 3D Image Rendering and Porosity Estimation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all subscribers:

I am looking to obtain a pore size distribution and a porosity estimate of a
sample from two scanning electron micrographs taken at a 10° offset from one
another. Both files are tiff format. All of the software I was able to find
uses z-stacking and not an angular offset. Does anyone have any
recommendations on software for generating a 3-D image and obtaining the
information? Thank you for your time.

Dan


****************************************************************************
******************************************************************
Daniel D. Smolko, Ph.D.
Senior Research Scientist & BioChemical Engineer
Nanogen, Incorporated
10398 Pacific Center Court
San Diego, CA 92121

Tel.# (858) 410-4798
Fax.# (858) 410-4848
e-mail: dsmolko-at-nanogen.com


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recipient(s) entity to which it is addressed. This message is confidential
and may contain information that is privileged, confidential and is exempt
from disclosure under applicable law. If you are not the intended
recipient(s), you may not review, copy, or distribute this message. If you
have received this communication in error, please notify us immediately by
e-mail, or telephone and delete the original message. Thank you.
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From daemon Thu May 9 13:14:17 2002



From: jaod2e-at-studentmail.umsl.edu
Date: Thu, 09 May 2002 13:06:54 -0500 (CDT)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to have my name removed from this listserve.
Thank You.


From daemon Thu May 9 13:29:10 2002



From: John W. Mattila :      jwmattil-at-iastate.edu
Date: Thu, 09 May 2002 13:18:19 -0500
Subject: FISH Class/Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

We have a visiting scientist interested in learning FISH techniques -- are
there any class(es)/workshops out there being offered in the near future
(ie. this summer or fall)?

TIA,

John

John W. Mattila
Iowa State University
Bessey Microscopy Facility
Room 1, Bessey Hall
Ames, IA 50011-1020

Ph: 515 294-3872
Fax: 515 294-1337




From daemon Thu May 9 14:44:42 2002



From: Michael Urbanik :      crystalguru-at-earthlink.net
Date: Thu, 9 May 2002 15:40:17 -0400
Subject: Re: Materials polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Malc,
We polish many different crystals and materials here.
We also build our own lapping and polishing equipment.
I thought I might be able to assist you in making a cost-effective
choice in selecting equipment if I were able to learn just exactly
what you need to do. We routinely achieve surfaces with as low
as 3 to 4 Angstrom RMS roughness here.
Let me know if I can be of help to you.

Cheers,
Michael Urbanik
} From the U.S.A., the keeper of the Flame of Freedom.
www.crystalguru.com


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} Hi.
} We're investigating various alternatives for the polishing of rocks
} of various kinds. One of the suggestions is to go the Struers' MD-system
} route, but as you can imagine this is the expensive option. Is there
} anyone out there using this and how is it working for you? My particular
} concerns are lif time of the discs and enbedding of foreign particles
} (mineral frags that may chip off) into the polymer surface rendering
} these unusable.
} Thanks,
} Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (try your luck!)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA







From daemon Thu May 9 16:31:40 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 9 May 2002 22:26:07 +0100
Subject: SEM - 2D to 3D Image Rendering and Porosity Estimation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dan
You may find the Soft Imaging System web site relevant. There is a
module for AnalySIS
that calculates quantitative elevation maps based on stereo pair
images

http://www.soft-imaging.de/eng/products/modules/stereo/ste_e.php

Chris

----- Original Message -----
} From: "Smolko, Dan" {DSmolko-at-Nanogen.com}
To: "'Microscopy-at-MSA.Microscopy.Com'"
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 09, 2002 6:21 PM


To all subscribers:

I am looking to obtain a pore size distribution and a porosity
estimate of a
sample from two scanning electron micrographs taken at a 10° offset
from one
another. Both files are tiff format. All of the software I was able
to find
uses z-stacking and not an angular offset. Does anyone have any
recommendations on software for generating a 3-D image and obtaining
the
information? Thank you for your time.

Dan


**********************************************************************
******
******************************************************************
Daniel D. Smolko, Ph.D.
Senior Research Scientist & BioChemical Engineer
Nanogen, Incorporated
10398 Pacific Center Court
San Diego, CA 92121

Tel.# (858) 410-4798
Fax.# (858) 410-4848
e-mail: dsmolko-at-nanogen.com


||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
||||||
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
||||||
|||||||||||||||||||||||||||||
This e-mail message is intended only for use by the individual, or
recipient(s) entity to which it is addressed. This message is
confidential
and may contain information that is privileged, confidential and is
exempt
from disclosure under applicable law. If you are not the intended
recipient(s), you may not review, copy, or distribute this message. If
you
have received this communication in error, please notify us
immediately by
e-mail, or telephone and delete the original message. Thank you.
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From daemon Thu May 9 17:44:38 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 9 May 2002 15:20:29 -0700
Subject: Re: LM, GMA resin, disposable blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} During several years I used Ralph Knifes to section Glycol Methacrylate
} embedded material (2-3 micrometer) for LM.
} Has anyone experience in sectioning with disposable blades?
}
} Thanks for the courtesy
} Bruno Dore

Bruno -

You can hand-break Ralph knives from glass microscope slides. They're
cheap, good, and disposable.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Thu May 9 17:54:13 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 9 May 2002 16:53:33 -0600
Subject: SEM - 2D to 3D Image Rendering and Porosity Estimation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dan,

what you are looking for is a stereo reconstruction program. There are
several available, we make on too. If you want more information, please
contact me offline at the address below.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Smolko, Dan [mailto:DSmolko-at-Nanogen.com]
Sent: Thursday, May 09, 2002 11:21 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


To all subscribers:

I am looking to obtain a pore size distribution and a porosity estimate of a
sample from two scanning electron micrographs taken at a 10° offset from one
another. Both files are tiff format. All of the software I was able to find
uses z-stacking and not an angular offset. Does anyone have any
recommendations on software for generating a 3-D image and obtaining the
information? Thank you for your time.

Dan


****************************************************************************
******************************************************************
Daniel D. Smolko, Ph.D.
Senior Research Scientist & BioChemical Engineer
Nanogen, Incorporated
10398 Pacific Center Court
San Diego, CA 92121

Tel.# (858) 410-4798
Fax.# (858) 410-4848
e-mail: dsmolko-at-nanogen.com


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recipient(s) entity to which it is addressed. This message is confidential
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From daemon Thu May 9 21:03:47 2002



From: DrJohnRuss-at-aol.com
Date: Thu, 9 May 2002 21:50:18 EDT
Subject: Re: SEM - 2D to 3D Image Rendering and Porosity Estimation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


in addition to the SIS stereo reconstruction module, there is a similar
routine that is part of Fovea Pro. See
http://ReindeerGraphics.com/foveapro2/surface.html


From daemon Fri May 10 08:30:22 2002



From: Thimothy Schneider :      timothy.schneider-at-mail.tju.edu
Date: Fri, 10 May 2002 09:23:30 -0400
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 JAH
1020 Locust Street
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cell
Timothy.Schneider-at-Mail.TJU.edu



From daemon Fri May 10 08:30:38 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Fri, 10 May 2002 09:24:05 -0400
Subject: rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


anyone out there have any experience using the edwards vacumm evaporator to
visualize macromolecules?
john



From daemon Fri May 10 08:46:37 2002



From: Y.Chen-at-surrey.ac.uk
Date: Fri, 10 May 2002 14:39:57 GB
Subject: Tungsten Filament in Philips EM400T

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

We have a Philips EM400T in our lab. It is old but still works well. However, I
noticed that illumination can be observed as soon as High Tension is on. As a
result, the saturation point is very low, only two or three steps of filament
current. My question is why there is a clear emmission current without any
filament current? Is this caused by a gun fault? What can we do about it?
Thank you very much in advance!

Yanling

Dr.Y.L.Chen
MicroStructural Studies Unit
School of Engineering
The University of Surrey
Guildford, Surrey
GU2 7XH, England


---------------------------------------------
This message was sent using UNIS MailSystem.




From daemon Fri May 10 09:10:57 2002



From: Gary Radice :      gradice-at-richmond.edu
Date: Fri, 10 May 2002 10:04:02 -0400
Subject: re: negative scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} } I am interested in the current negative-scanner
} } technology, but I would like to find a non-contact
} } scanner.

If you have a robust budget, (12-18K$) you might check the Imacon
scanners, either the Flextite 848 or Precision III.

I have no commercial interest in these products.
--
Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice


From daemon Fri May 10 11:18:07 2002



From: JoAn Hudson :      hudson-at-uoneuro.uoregon.edu
Date: Fri, 10 May 2002 09:17:27 -0700
Subject: Book Reviews

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for individuals who are willing to review and write a
reviewed article of the following books for the MSA Journal.

1. "Electron Microscopy and Analysis 2001" (A materials related
book). Edited by M. Aindow and C.J. Kiely
2. "Luminescence Biotechnology Instruments and Applications" Edited
by Knox Van Dyke, Christopher Van Dyke and Karen Woodfork
3. " Photography with a Microscope" Fred Rost and Ron Oldfield
4. " Selected Papers on Optical Low-Coherence Reflectometry &
Tomography" Edited by Barry R. Masters
5. "Near-Infrared Technology in the Agricultural and Food
Industries" Edited by Phil Williams and Karl Norris

If you are interested in participating please send me the title of the
book you would like to review and it will be sent to you ASAP. Selection
will be make on a first come first serve basis.

Thank you!

JoAn Hudson, PhD.
Institute of Neuroscience
222 Huestis Hall
University of Oregon,
Eugene, OR 97403
 
Telephone: (541) 346 4508
FAX: (541) 346 4548






From daemon Fri May 10 12:50:35 2002



From: Antonio Correia :      antonio-at-cmp-cientifica.com
Date: Fri, 10 May 2002 19:39:26 +0200
Subject: TNT2002 Conference - Deadlines (40 graduate grants available)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleague,

Please find enclosed deadlines related to TNT2002 conference to be held in Santiago de Compostela (Spain): September 9-13, 2002:

!!May 17, 2002: Extended "Graduate grant request" deadline!!
!!May 24, 2002: Extended Abstract submission deadline!!
June 03, 2002: Notification to the authors by e-mail of the accepted papers for oral presentation or poster / Notification of graduate travel awards
June 17, 2002: Registration deadline
July 01, 2002: Preliminary programme
July 15, 2002: Final programme & "Extended Abstracts" booklet printing
September 09, 2002: Manuscript submission deadline (to be published in "Nanotechnology" journal)

40 Grants for students are available: 20 for US and 20 for Europe - please check http://www.cmp-cientifica.com/cientifica/frameworks/generic/public_users/TNT02/TNT02_grants.html for more info.

If you are interested in presenting an oral contribution, please send me (mailto:antonio-at-cmp-cientifica.com) as soon as possible a tentative title talk and an extended abstract (2 pages max including figures) - only a few slots are still available. Therefore, the committee will select papers for oral presentation and we will notify authors shortly. We encourage participants to present posters during TNT2002.

If you need more information, please do not hesitate to contact me. If you accept this invitation, please respond by email as soon as possible.

Hoping to receive a positive answer to our invitation.

Antonio Correia

-----------------------------------------------------------------------------------
Dr. Antonio CORREIA - Coordinator of the IST Nanoelectronics Network (PHANTOMS)
CMP Cientifica S.L.
Phone: +34 91 6407187 Fax: +34 91 6407186
mailto:antonio-at-cmp-cientifica.com
WEB site: http://www.cmp-cientifica.com/
PHANTOMS WEB site: http://www.phantomsnet.com/
TNT2002: http://www.cmp-cientifica.com/TNT2002.html

------------------------------------------------------------------------------------
Please find enclosed confirmed TNT02 Keynote Speakers (09/05/2002):

1. Masakasu Aono (Riken, Japan)
2. Phaedon Avouris (IBM, USA)
3. Yoshio Bando - National Institute for Materials Science (NIMS) (Japan)
4. Flemming Besenbacher (Aarhus University, Denmark)
5. Mark Blamire (University of Cambridge, UK)
6. Guillermo Bozzolo (NASA Glenn Research Center, USA)
7. George Bourianoff (Intel, USA)
8. Roberto Car (Princeton University, USA)
9. Ignacio Cirac (Max-Planck Institut fur Quantenoptik, Germany)
10. Dongmin Chen (Rowland Institute for Science, Cambridge, MA, USA)
11. Wonbong Choi (Samsung, Korea)
12. Harold Craighead (Cornell University, USA)
13. Supriyo Datta (Purdue University, USA)
14. Cees Dekker (Delft University, Netherlands)
15. Pedro Echenique (Donostia International Physics Center (DIPC), Spain)
16. Andreas Engel (Basel University, Switzerland)
17. Leo Esaki (Shibaura Institute of Technology, Japan) - Invited Lecture
18. Fernando Flores (Universidad Autonoma de Madrid, Spain)
19. Harald Fuchs (Munster University, Germany)
20. Christoph Gerber (IBM, Switzerland)
21. James Gimzewski (UCLA, USA)
22. Herb Goronkin (Physical Research Laboratory - Motorola Labs, USA)
23. Masahiko Hara (Tokyo Institute of Technology, Japan)
24. James Heath (UCLA, USA)
25. Kikuji Hirose (Osaka University, Japan)
26. Christian Joachim (CEMES/CNRS, France)
27. Sajeev John (University of Toronto, Canada)
28. Dieter Kern (Tuebingen University, Germany)
29. Uzi Landman (Georgia Institute of Technology, USA)
30. Stuart Lindsay (Arizona State University, USA)
31. Daniel Loss (Basel University, Switzerland)
32. Ramesh G. Mani (Harvard University, USA)
33. Meyya Meyyappan (NASA, USA)
34. Seizo Morita (Osaka University, Japan)
35. Rodolfo Miranda (Universidad Autónoma de Madrid, Spain)
36. Gernot Pomrenke (Air Force Office of Scientific Research, USA)
37. Calvin Quate (Stanford University, USA)
38. Jan van Ruitenbeek (Leiden University, Netherlands)
39. Lars Samuelson (Lund University, Sweden)
40. Christian Schoenenberger (Basel University, Switzerland)
41. Ivan Schuller (University of California, USA)
42. Clivia Sotomayor Torres (Wuppertal University, Germany)
43. Shen Tsai (NEC Fundamental Research Laboratories, Japan)
44. Christian Urbina (CEA-Saclay, France)
45. Luis Vina (Universidad Autonoma de Madrid, Spain)
46. Mark Welland (University of Cambridge, UK)
47. Stanley Williams (HP, USA)



From daemon Fri May 10 14:33:32 2002



From: George_Munzing-at-engelhard.com
Date: Fri, 10 May 2002 15:27:27 -0400
Subject: Gatan 628/2 Environmental Holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings all.

I have come across a Gatan model 628/2 single tilt hot stage (1300C) and
heating control unit that was purchased for a Hitachi H600 100Kv TEM but
was never used. It was apparently tucked away in the lab by my predecessor
immediately after its purchase and was recently discovered after having
already replaced this microscope. I would like to know if anyone with a
similar microscope is interested in actually using it.

Please contact me off-line to discuss in more detail.

George R. Munzing Jr.
Engelhard Corporation
25 Middlesex-Essex Tpk.
Iselin, NJ 08830
TELE 732-205-7030
FAX 732-205-5300



From daemon Fri May 10 15:34:22 2002



From: Corazon D. Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Fri, 10 May 2002 15:22:03 -0400
Subject: slide presentation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate hearing from people taking color digital images. Is
there another slide presentation software, other than Powerpoint, that one
can use to show histology or fluorescence micrographs in a lecture or
meeting. The problem is pasting high resolution images to a Powerpoint
slide - quite often we have to reduce the size or the resolution of the
images and the images appear washed out. It defeats the purpose of using
high resolution cameras to capture images. Any
comments/suggestions/recommendations would be greatly appreciated.

Cora Bucana

Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747


From daemon Fri May 10 17:10:19 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 10 May 2002 15:01:38 -0700
Subject: Re: rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try this:
http://www.google.com/search?hl=en&ie=UTF8&oe=UTF8&q=edwards+vacuum+evaporator&btnG=Google+Search


At 06:24 AM 5/10/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri May 10 18:46:04 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 10 May 2002 16:37:20 -0700
Subject: Re: slide presentation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cora

If you need some program just to show already prepared images (slide show)
you may try InfanView - this freeware software were discussed recently on
ListServer and received very high marks. Again, it's only for
demonstration, you may not create PowerPoint-like presentation in it. You
may show already prepared (even in Powerpoint I believe, not sure)
images. It's very good 'viewer'. It has some very nice features like - it
may shrink your image to the screen size and it's so quick. I am using it
to show my EM pictures 20-50 Mb in size...
http://www.irfanview.com/
Sergey

At 12:22 PM 5/10/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri May 10 18:50:48 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 10 May 2002 19:44:58 -0400
Subject: slide presentation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would wager that the problem isn't with PowerPoint, but rather the projector. Consider that most of the projectors in use now are 1024 x 728 pixels. The whole PowerPoint slide takes that up. If your slide in PowerPoint is set up for 10" x 7.5" (the default) and your image is put on the slide as a 4"x5", then whatever resolution your image was taken at is still only projected at 512x364 pixels.

Now consider using overheads -still not the best, but let's say that you have a good printer that you can print at 300 dpi. (Sublimation dye printers can do this and good quality inkjets can come close to doing this with 256 levels per color.) A 4"x5" print on the slide can still have a size of 1200 x 1500 pixels without loosing anything.

Add to this, the problems of projecting the full color range with projectors. I have read about this, but am not qualified to discuss it.

My advice is to stay with overhead transparencies and forget the fancy slide effects that you can do with PowerPoint or other presentation software.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Corazon D. Bucana [mailto:bucana-at-audumla.mdacc.tmc.edu]
Sent: Friday, May 10, 2002 3:22 PM
To: Microscopy-at-sparc5.microscopy.com


I would appreciate hearing from people taking color digital images. Is
there another slide presentation software, other than Powerpoint, that one
can use to show histology or fluorescence micrographs in a lecture or
meeting. The problem is pasting high resolution images to a Powerpoint
slide - quite often we have to reduce the size or the resolution of the
images and the images appear washed out. It defeats the purpose of using
high resolution cameras to capture images. Any
comments/suggestions/recommendations would be greatly appreciated.

Cora Bucana

Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747


From daemon Fri May 10 18:55:30 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Fri, 10 May 2002 19:49:31 -0400 (EDT)
Subject: Re: slide presentation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Corazon....

You might try using the slideshow function available in Irfanview
freeware.

www.irfanview.com

Best,

Angela

-----------------------------------------
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------

On Fri, 10 May 2002, Corazon D. Bucana wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would appreciate hearing from people taking color digital images. Is
} there another slide presentation software, other than Powerpoint, that one
} can use to show histology or fluorescence micrographs in a lecture or
} meeting. The problem is pasting high resolution images to a Powerpoint
} slide - quite often we have to reduce the size or the resolution of the
} images and the images appear washed out. It defeats the purpose of using
} high resolution cameras to capture images. Any
} comments/suggestions/recommendations would be greatly appreciated.
}
} Cora Bucana
}
} Corazon D. Bucana, Ph.D.
} Dept. Cancer Biology
} UT M.D.Anderson Cancer Center
} 1515 Holcombe Blvd, Box 173
} Houston, Texas 77030
} Phone: 713-792-8106
} FAX 713-792-8747
}
}



From daemon Fri May 10 19:44:53 2002



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Fri, 10 May 2002 17:37:59 -0700
Subject: RE: slide presentation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Cora,
I've used a piece of software called "UPresent" - its very good for
multi-media work, but the last version I used was a little light on text and
outlining capabillities. Here is the URL:

http://www.codeblazer.com/products.html

-Brad

----------
From: Corazon D. Bucana
Sent: Friday, May 10, 2002 12:22 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: slide presentation software


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.


I would appreciate hearing from people taking color digital images.
Is
there another slide presentation software, other than Powerpoint,
that one
can use to show histology or fluorescence micrographs in a lecture
or
meeting. The problem is pasting high resolution images to a
Powerpoint
slide - quite often we have to reduce the size or the resolution of
the
images and the images appear washed out. It defeats the purpose of
using
high resolution cameras to capture images. Any
comments/suggestions/recommendations would be greatly appreciated.

Cora Bucana

Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747




From daemon Sat May 11 07:42:01 2002



From: barbara maloney :      bmalon01-at-fiu.edu
Date: Sat, 11 May 2002 08:27:15 -0400
Subject: grainy image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everybody - using a JEOL 5900LV, recently replaced tungsten filament,
got great images, few days later image looked like I was in backscatter
mode instead of secondary. Stubs were coated 360ang of Au, stubs are
flush with sample holder; 20kv, WD 8mm, ss 37 - looking at diatoms
1)aligned gun
2)checked the wobbler
Any suggestions of what is going on?
Thanks
Barb



From daemon Sat May 11 10:44:54 2002



From: Larry Hanke :      hanke-at-mee-inc.com
Date: Sat, 11 May 2002 10:36:59 -0500
Subject: Re: slide presentation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From you post, I get the impression that you are reducing
the resolution of the image before inserting into
Powerpoint. This is one way to make image fit into the slide
image area, but does decrease the resolution of the
projected image. You can insert the image at full
resolution, then size the image on the slide using the
Format Image function.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870




From daemon Sat May 11 19:49:21 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Sat, 11 May 2002 19:39:24 -0500
Subject: Re: slide presentation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I second Scott Walck's comments and add a thought.

The limitation is most likely the projector technology. You are limited to
1024x768 resolution input on the newer projectors. There might still be a
question as to whether all that input ends up as output. I have an adapter
to take computer input (maybe even up to 1280x1024) and convert it to make
it suitable for a video projector. But that projector can only handle NTSC
which is a little worse that VGA (640x480) resolution. So all I put in
definitely does not make it out there. I also know the color rendition is
rather poor.

Scott suggested overheads as an option. I agree and might suggest 35mm
slides as another alternative. Our campus has (or at least had) a
centralized slide maker that could take Power Point or any printed output
and render it at 4000x3000 pixels, IIRC.

But will your audience be able to resolve that much detail from where they
are sitting?

I would like to hear from someone who can speak authoritatively on the
physiology of the matter. My own sense is that anything more than 1024
pixels across will not be appreciated from normal viewing distance.
1024-pixel images may seem pixelated to someone standing a few feet away
from the screen, but I doubt that a viewer sitting in the front row would
notice.

For that reason, I take the approach of simply recording multiple images
from our SEMs at 1024-pixels across showing the appropriate level of detail
in each image. A 40-MB image certainly contains lots of information;
however, it can really only be appreciated one 1024-pixel image at a time.
I suggest that is the way the presentations will need to be prepared.

At 03:22 PM 5/10/02 -0400, you wrote:

} I would appreciate hearing from people taking color digital images. Is
} there another slide presentation software, other than Powerpoint, that one
} can use to show histology or fluorescence micrographs in a lecture or
} meeting. The problem is pasting high resolution images to a Powerpoint
} slide - quite often we have to reduce the size or the resolution of the
} images and the images appear washed out. It defeats the purpose of using
} high resolution cameras to capture images. Any
} comments/suggestions/recommendations would be greatly appreciated.
}
} Cora Bucana
}
} Corazon D. Bucana, Ph.D.
} Dept. Cancer Biology
} UT M.D.Anderson Cancer Center
} 1515 Holcombe Blvd, Box 173
} Houston, Texas 77030
} Phone: 713-792-8106
} FAX 713-792-8747

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Mon May 13 01:01:42 2002



From: Cathy Gillespie :      cathy.gillespie-at-anu.edu.au
Date: Mon, 13 May 2002 15:46:58 +1000
Subject: TEM samples - how long?

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I am interested in opinions on how long it takes to prepare a routine
chemically fixed, resin embedded biological sample for TEM (by an average
technician). I need to work out how many samples could reasonably be
prepared by one person in a year.

Thanks
Cathy Gillespie





From daemon Mon May 13 01:01:42 2002



From: Cathy Gillespie :      cathy.gillespie-at-anu.edu.au
Date: Mon, 13 May 2002 15:46:58 +1000
Subject: TEM samples - how long?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in opinions on how long it takes to prepare a routine
chemically fixed, resin embedded biological sample for TEM (by an average
technician). I need to work out how many samples could reasonably be
prepared by one person in a year.

Thanks
Cathy Gillespie





From daemon Mon May 13 04:31:51 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 13 May 2002 10:25:52 +0100 (GMT Daylight Time)
Subject: Re: Tungsten Filament in Philips EM400T

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Hi Chen,

I don't know the 420 but as there have been no other
responses maybe I can shed some light on your question.

} From memory, when we had our Philips 300 the filament was
always on at a low level, the filament control increased
this level but never switched it off. I guess it was
interlocked with the vacuum or something, I'm a little hazy
about the details. On the back of the console was a switch
(I think marked high and low) that would adjust the current
setting.

It sounds like your 420 is similar, ie. there is a heating
current even though the filament control is off. As soon
as you have HT you have emission. It may be that this batch
of filaments have slightly thinner wire or that the
filament is not set correctly in the Wenhelt. If the
filament is set correctly and there is a switch for the
filament current that is set on 'high' try setting it to
'low'. If you can still saturate the filament on that
setting then that's OK.

Maybe the quiescient current circuit is playing up and
giving too high a current?

If that is not the case then I have no idea how you can get
a beam without heating unless you have field emission from
a very sharp tip on the filament. Not that likely at 120kV
but seen at 1MeV on HVEMs.

Good luck,
Ron

On Fri, 10 May 2002 14:39:57 GB
"Y.Chen-at-surrey.ac.uk"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} We have a Philips EM400T in our lab. It is old but still works well. However, I
} noticed that illumination can be observed as soon as High Tension is on. As a
} result, the saturation point is very low, only two or three steps of filament
} current. My question is why there is a clear emmission current without any
} filament current? Is this caused by a gun fault? What can we do about it?
} Thank you very much in advance!
}
} Yanling
}
} Dr.Y.L.Chen
} MicroStructural Studies Unit
} School of Engineering
} The University of Surrey
} Guildford, Surrey
} GU2 7XH, England
}
}
} ---------------------------------------------
} This message was sent using UNIS MailSystem.
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Mon May 13 04:40:35 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 13 May 2002 02:51:32 -0700
Subject: Re: TEM samples - how long?

Contents Retrieved from Microscopy Listserver Archives
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Kathy,
It'll depend, how much you'll pay for it. If you offer $50-60K, S/he will
make a LOT of samples to you!!! There is no 'average' technician in
EM. EM is very sophisticated area and in order to have necessary
knowledge, experience etc, you have to spend 2-3-5 years. I am not talking
about 'hands' - it comes from God. It's not easy to find such qualified
person. 'Average' person will kill your most valuable sample in two
minutes... Good luck. Sergey

At 03:46 PM 5/13/02 +1000, you wrote:
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 13 07:24:49 2002



From: =?iso-8859-1?q?Jeremy=20Sanderson?= :      jb_sanderson-at-yahoo.com
Date: Mon, 13 May 2002 13:16:26 +0100 (BST)
Subject: JPEG file recovery

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I am looking for programmes (preferably free!)that
will enable me to read jpeg files recovered after
deletion, or corruption of the file marker. I have
tried a few of the better known jpeg file readers
without success. Thanks, Jeremy
Reply to jb_sanderson-at-yahoo.com


__________________________________________________
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from News and Sport to Email and Music Charts
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From daemon Mon May 13 10:19:30 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Mon, 13 May 2002 10:10:45 -0500
Subject: Re: negative scanners

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Hi All,

If you want a scanner for smaller negs(35mm or slides), we have a Nikon
Super Coolscan 4000 scanner that does quite well and costs less. I
think it cost between 1&2K. Less expensive scanners I've used tend
to put lines in the image or have missing pixels.

Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas

Gary Radice wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } } I am interested in the current negative-scanner
} } } technology, but I would like to find a non-contact
} } } scanner.
}
} If you have a robust budget, (12-18K$) you might check the Imacon
} scanners, either the Flextite 848 or Precision III.
}
} I have no commercial interest in these products.
} --
} Gary P. Radice gradice-at-richmond.edu
} Associate Professor of Biology 804 289 8107 (voice)
} University of Richmond 804 289 8233 (FAX)
} Richmond VA 23173 http://www.science.richmond.edu/~radice



From daemon Mon May 13 10:29:57 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Mon, 13 May 2002 11:19:50 -0400
Subject: Platinum shadowing thickness

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Microscopists,
Does anyone know of a way (quant. or qualitative) to meause the
amount of thickness coating a protein sample with platinum in a metal
evaporation
run? I don't have a quartz thickness monitor, so is there some
extrapolation you can do based on starting and ending material? Right
now all we do is
use filter paper wedges to estimate shadow thickness (not empirically).
Thank you.

Mike D.



From daemon Mon May 13 11:29:26 2002



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Mon, 13 May 2002 12:21:51 -0400
Subject: Platinum shadowing thickness

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Funny you should ask. I wrote a post last night but wasn't going to post it until tomorrow.

In the late 1960s there were charts called nomographs to graphically estimate the thickness of films evaporated onto substrates. They were kind of a graphical slide rule to figure out how much metal to evaporate onto a substrate.
I can post a better email from home but this is the link to a nomograph on the web.

http://www.technology.niagarac.on.ca/courses/phtn1432/NotesOnPreloadingSources.html

The scale at the top is the bulk density of the element or alloy. It is not labeled with the number scale, only the elements. Neither is carbon (near Be), Ge and some alloys like Au-Pd shown.

The formula used for these nomograph charts and converted to use bulk densities is:

wt = [4 * Pi * d² * t * rho * (10^-5)]/(sin s)

wt to use is in mgs
d is the distance from the point source to the sample target in cms.
t is the thickness in angstroms, Å.
rho is the bulk density, 21.45 grams per cm³ for Pt.
10 EE -5 is to get the units right.
s is the shadowing angle measured at the target (as I recall) and that is subtended by the point source and the plane of the sample.

It is easy to derive this formula from surface area concepts and densities.

For straight down evaporations, sin 90º = 1. The above nomograph doesn't have a scale for shadowing angle. The formula will tell you how to correctly ESTIMATE the amount of material you used.

Hope this helps.

Paul Beauregard
Senior Research Associate
PPG Industries
Monroeville Technical Center
Monroeville, PA 15146



-----Original Message-----
} From: Mike Delannoy [mailto:delannoy-at-jhmi.edu]
Sent: Monday, May 13, 2002 11:20 AM
To: microscopylistserver


Microscopists,
Does anyone know of a way (quant. or qualitative) to meause the
amount of thickness coating a protein sample with platinum in a metal
evaporation run?
I don't have a quartz thickness monitor, so is there some
extrapolation you can do based on starting and ending material? Right
now all we do is use filter paper wedges to estimate shadow thickness (not empirically).
Thank you.

Mike D.



From daemon Mon May 13 14:50:49 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 13 May 2002 12:58:43 -0700
Subject: Re: JPEG file recovery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Jeremy

Is it possible to recover corrupted JPEG file specifically? Never hear
about that. I could talk about PC/Win platform only. Windows NT itself
(NTFS file system) has internal ability to recover corrupted files
(any). It's called 'checkdisk' I believe. It's in the standard Windows
package. Similar things should be in win2000. You may also run Norton Disk
Doctor on any Win platform - it works great. The things about that: all
these programs are not specific to the JPEG files. So, I am not sure how
it may help in your particular case. By the way, for PC/Win I would
strongly recommend to have Norton Utilities on HD: very useful stuff. Sergey

At 01:16 PM 5/13/02 +0100, you wrote:
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 13 15:59:45 2002



From: Leland G Hanna :      Leland.G.Hanna-at-usa.dupont.com
Date: Mon, 13 May 2002 16:49:03 -0400
Subject: Re: Tungsten Filament in Philips EM400T

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Greetings Folks,

I'd like to echo Ron's advice regarding checking that the filament is set
up correctly in the wehnelt and that it meets the manufacturers specs
(thickness, material, etc). The type of problem you described can be caused
by any number of faults in the HT circuitry of your 400T, but your first
and most likely source, and easiest and least expensive to fix, would be a
filament exchange. If that doesn't cure your problem I would recommend
having a qualified service engineer in to analyse the situation. We once
had a similar problem on our CM12 (which has a nearly identical HT set) and
after cleaning the emission chamber, cleaning then replacing the gun, and
just before exchanging the HT generator tank, we were able to prove that
our cable was breaking down at voltages greater than 40KV. This created all
kinds of bias problems which manifested with the symptoms you have
described. When the problem first started occurring, it was suspected that
we had a malfunction in the emission section of our HT tank. The filaments
could be set very far back in the wehnelt assembly to achieve somewhat
"normal" heating and illumination behaviour, but changing the Emission
setting then had negligible impact on beam current. If these symptoms are
what you are noticing with your TEM, you might want to check the HT cable.
This is probably best accomplished with a meter that will measure
inductance, checking for high impedance "short circuits" amongst the three
conductors of the cable. Considerable disassembly may be required to do
this properly, (to isolate both ends of the cable) hence the
recommendation to call in the service representative.

Good Luck!!!

Lee



From daemon Mon May 13 17:49:04 2002



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Mon, 13 May 2002 18:40:57 -0400
Subject: Re: Tungsten Filament in Philips EM400T

Contents Retrieved from Microscopy Listserver Archives
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On our Philips 400T we had a switch on the back of the left console
that was marked Low/High, the low settng was used for tungsten
filaments and the high setting for LaB6 filaments which required more
current to heat the crystal. It may be that this switch was
accidently bumped to the high position?





At 4:49 PM -0400 5/13/02, Leland G Hanna wrote:
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--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html


From daemon Mon May 13 18:29:39 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 13 May 2002 16:24:07 -0700
Subject: Re: JPEG file recovery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey points out some good facets of file recovery.

What is missing from your post is whether you are using
FAT16, FAT32 or NTFS. In a "normal" mode, the OS
does not delete the file, but rather, it "marks" it as deleted.
This is done by replacing the file name with an initial ? mark.
There are variations on this theme. But unless and until
the disk is defragmented, the original file is still on the drive.
(a good reason to use Norton WipeDisk if you don't want
it to remain there) But there are exceptions, of course.

I agree that Norton DiskDoctor is the way to go. This will
allow recovery (un-deletion) of files. But beware that if you
delete a file and do other disk actions, recovery may be
impossible. This is because the prior-used sectors are now
marked as available for use. So if you delete a file, then
add new files, all or portions of your deleted file's sectors
could be used--eliminating any possibility of recovery.

gary g.


At 12:58 PM 5/13/2002, you wrote:

} Hello Jeremy
}
} Is it possible to recover corrupted JPEG file specifically? Never hear
} about that. I could talk about PC/Win platform only. Windows NT itself
} (NTFS file system) has internal ability to recover corrupted files
} (any). It's called 'checkdisk' I believe. It's in the standard Windows
} package. Similar things should be in win2000. You may also run Norton
} Disk Doctor on any Win platform - it works great. The things about that:
} all these programs are not specific to the JPEG files. So, I am not sure
} how it may help in your particular case. By the way, for PC/Win I would
} strongly recommend to have Norton Utilities on HD: very useful stuff. Sergey
}
} At 01:16 PM 5/13/02 +0100, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 13 18:46:24 2002



From: Smolko, Dan :      DSmolko-at-nanogen.com (by way of MicroscopyListserver)
Date: Mon, 13 May 2002 18:36:57 -0500
Subject: 2D to 3D image conversion

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Thank you for all of your input!

I finally found the software I was looking for. This information was
forwarded to me by Michael Nesson, Ph.D. at OSU and a similar message was
sent by John Mardinly.

"The company is called Alicona Imaging GmbH. The software product is called
MeX. Try www.alicona.com for additional information."

A demo version is available online and the software looks very impressive
but the cost is several $K. Thank you for the help.

Dan

****************************************************************************
******************************************************************
Daniel D. Smolko, Ph.D.
Senior Research Scientist & BioChemical Engineer
Nanogen, Incorporated
10398 Pacific Center Court
San Diego, CA 92121

Tel.# (858) 410-4798
Fax.# (858) 410-4848
e-mail: dsmolko-at-nanogen.com


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From daemon Mon May 13 23:24:30 2002



From: Corazon D. Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Mon, 13 May 2002 23:10:06 -0400
Subject: slide presentation

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Thank you to all who responded to my request for information regarding
other software for slide presentation. Some of the suggestions include:
(a) http://www.irfanview.com I downloaded this tonight and I find it very
easy to use.
(b) UPresent from http://www.codeblazer.com/products.html I have not
tried this yet
(c) Sliderite
(d) using HTML
(e) JPEGView
(f) Other suggestions deal with increasing the computer RAM and data
compression.


Our projector has a 1024 x768 resolution and we do paste the full image
onto the Powerpoint page and then adjust the size of the image but the
problem comes when we try to paste a composite consisting of more than 6
images. Quite often the images appear washed out - the images were
captured originally in Tiff format but as I indicated earlier we had to
compress the file before pasting.

Thank you all for the information. I will try some of the other
suggestions soon.

Cora Bucana




Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747


From daemon Tue May 14 07:11:15 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Tue, 14 May 2002 07:57:17 -0400
Subject: Re: TEM samples - how long?

Contents Retrieved from Microscopy Listserver Archives
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Here here, i recently left clinical area for reseach. the head cyto tech
convinced the administrator that he could do the job as good as i could. in
six months he trashed the lab 3 diamond knives, and an LKB. an average EM
tech. is probly a bad em tech.
as for your question. i have seen labs that do 1000/year with just 1.5 techs.
i wouldn't want to work in one of those mills.
john


From daemon Tue May 14 07:58:40 2002



From: Eric Johnston :      ericdj-at-seas.upenn.edu
Date: Tue, 14 May 2002 08:50:47 -0400
Subject: Hot spot in epifluorescence

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Hi

Long time lurker, first time poster (well, almost).

We recently upgraded from a video camera to a cooled digital camera that is
very sensitive. While doing epifluorescence on our Nikon TE300, we noticed
some rather significant variations in the illumination from the mercury
lamp, specifically a hot spot filling about 1/4 of the screen. This is not
visible through the eyepiece and is not visible with the video camera. I
have tried re-aligning the lamp in various ways. This moves the hot spot
around but cannot make it go away. I even tried putting a diffuser in the
light path. The hot spot is still there. I've had the vendors in, who say
we might need a lamp with a bigger arc to fill the field of view.

Does anybody have any suggestions?

Eric



From daemon Tue May 14 09:54:12 2002



From: Oparowski, Joseph :      Joseph_Oparowski-at-bose.com
Date: Tue, 14 May 2002 10:46:53 -0400
Subject: AMRay installation

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Greetings,

The following note is posted for a friend who does not have access to the
list server;

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313




A colleague is looking for someone to install an Amray
3600 FESEM. Please reply directly to

James Greer
PVD Products
231 Andover St.
Wilmington, MA 01887
(978) 694-9455
jgreer-at-pvdproducts.com

Thanks,
Audrey Dow


From daemon Tue May 14 10:46:09 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Tue, 14 May 2002 11:46:42 -0400
Subject: Re: Hot spot in epifluorescence

Contents Retrieved from Microscopy Listserver Archives
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Eric,

You might be imaging a reflection off of the front of some part of the
camera/lens system. Check to make sure that there is no contamination on the
forward side of any of the accessible optical elements that could serve as a
source of scattered light that would be reflected by another optical element.

John Twilley

Eric Johnston wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi
}
} Long time lurker, first time poster (well, almost).
}
} We recently upgraded from a video camera to a cooled digital camera that is
} very sensitive. While doing epifluorescence on our Nikon TE300, we noticed
} some rather significant variations in the illumination from the mercury
} lamp, specifically a hot spot filling about 1/4 of the screen. This is not
} visible through the eyepiece and is not visible with the video camera. I
} have tried re-aligning the lamp in various ways. This moves the hot spot
} around but cannot make it go away. I even tried putting a diffuser in the
} light path. The hot spot is still there. I've had the vendors in, who say
} we might need a lamp with a bigger arc to fill the field of view.
}
} Does anybody have any suggestions?
}
} Eric





From daemon Tue May 14 13:30:22 2002



From: giummc-at-rpi.edu (by way of MicroscopyListserver)
Date: Tue, 14 May 2002 13:19:35 -0500
Subject: Ask-A-Microscopist: Detecting Ca in Al

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (giummc-at-rpi.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, May
14, 2002 at 12:37:15
---------------------------------------------------------------------------

Email: giummc-at-rpi.edu
Name: Cindie Giummarra

Organization: Rensselaer Polytechnic Institute

Education: Graduate College

Location: Troy, New York, USA

Question: Hi,

I was wondering which techniques I could use to detect calcium in
15-40ppm amounts in an aluminum alloy?

I am interested in finding out what form the Ca is in and where it is
located in the microstructure.

Thank you.

---------------------------------------------------------------------------


From daemon Tue May 14 14:04:33 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Tue, 14 May 2002 14:58:21 -0400
Subject: Biosafe adhesive

Contents Retrieved from Microscopy Listserver Archives
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O.k. this is not mciroscopy directly BUT I hope someone out there has an
answer.

We're looking for an "adhesive" to glue #3 Whatman filter paper to
stainless steel (surface area of ~ 3 sq cm or less) but it must be: (1) sterile or
sterilizable, (2) water stable, (3) biologically non-toxic.

Thanks for your suggestions!



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Tue May 14 14:05:05 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Tue, 14 May 2002 14:59:37 -0400
Subject: Cameras & Microscope Heads

Contents Retrieved from Microscopy Listserver Archives
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HI Listers,

We have an old Nikon Labophot2 and we are in need of a trinoc head. It is currently a dual head scope but we need to convert it so it can handle a digital camera. Currently we have a TriPix RGB (hanging off one of the eyepieces with an adapter) but we're having trouble with dust and spots at 100X oil. I know this is not the optimum configuartion.

Does any one have a suggestion about digital cameras for light microscopes? We are working mostly with H&E (10 -40X) and some blood smears (100X oil). We will be saving images to a website.

Also, I'm looking for anybody who might have a old trinoc head for a Nikon Labophot2 sitting around that might want to sell it.

I'm welcome to all ideas, so send them along.


In the new land of tornadoes,



Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Tue May 14 17:18:25 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 14 May 2002 15:10:55 -0700
Subject: Re: Biosafe adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Silicone or epoxy compound? Both of them are 'naturally' aseptic-
aggressive enough to kill any bacteria unless polymerized.... Silicone
when completely polymerized (be sure) is bio-compatible. I mean silicone
compound with clear silicone base and strong smell of acetic acid. Sergey

At 11:58 AM 5/14/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue May 14 17:49:12 2002



From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Mon, 13 May 2002 16:15:50 -0700
Subject: Re: slide presentation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I haven't had a chance to test this but I saw a very impressive
demonstration by Adobe using their Acrobat program (not the free Acrobat
Reader). Creating PDF files and editing them with this software is quite
easy and allows almost any program for creation.



From daemon Tue May 14 22:36:36 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 14 May 2002 23:23:02 -0700
Subject: Re: Biosafe adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Also, properly cured Silicone compounds mostly compatible with hot water,
but not with hot steam on repeated exposure, especially pressure seals.
Sterilize by boiling, not autoclaving. Try www.nusil.com for more
information.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, May 14, 2002 6:10 PM


Surprising: silicone rubber (after polymerization of the silicone compound)
is extremely stable against the temperature. It widely used in high
temperature applications (simplest example: silicone compound for car
engines). 121oC which is typical autoclave temperature should not hurt
silicone at any instances even with overheated steam... I also check WEB
site you suggested and did not find anything about thermal instability of
the silicones... Moreover, the curing temperature they suggested is 150oC
for 5 hours, which is much longer than autoclave procedure (20-30 min
usually). If I remember correctly, people used silicone compound to make
molds for the metal... I am sorry, could not agree with you: as I know,
silicone is most thermally stable rubber suitable for biological/medical
use. Actually, my point was that if you perform procedure at the sterile
conditions (laminar cabinet with HEPA filter for instance) you don't have
to sterilize the compound. Compound is sterile itself. You DO have to
care about sterility of the other parts, but non-polymerized silicone. Sergey

At 08:26 PM 5/14/02, you wrote:
} Also, properly cured Silicone compounds mostly compatible with hot water,
} but not with hot steam on repeated exposure, especially pressure seals.
} Sterilize by boiling, not autoclaving. Try www.nusil.com for more
} information.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
} ----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, May 14, 2002 6:10 PM
} Subject: Re: Biosafe adhesive
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Silicone or epoxy compound? Both of them are 'naturally' aseptic-
} } aggressive enough to kill any bacteria unless polymerized.... Silicone
} } when completely polymerized (be sure) is bio-compatible. I mean silicone
} } compound with clear silicone base and strong smell of acetic acid. Sergey
} }
} } At 11:58 AM 5/14/02, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } O.k. this is not mciroscopy directly BUT I hope someone out
} there
} } } has an
} } } answer.
} } }
} } } We're looking for an "adhesive" to glue #3 Whatman filter paper
} to
} } } stainless steel (surface area of ~ 3 sq cm or less) but it must be: (1)
} } } sterile or
} } } sterilizable, (2) water stable, (3) biologically non-toxic.
} } }
} } } Thanks for your suggestions!
} } }
} } }
} } }
} } } Richard E. Edelmann, Ph.D.
} } } Electron Microscopy Facility Supervisor
} } } 352 Pearson Hall
} } } Miami University, Oxford, OH 45056
} } } Ph: 513.529.5712 Fax: 513.529.4243
} } } E-mail: edelmare-at-muohio.edu
} } } http://www.emf.muohio.edu
} } }
} } } "RAM disk is NOT an installation procedure."
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
} }

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed May 15 04:29:13 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Wed, 15 May 2002 11:22:04 +0200
Subject: GIF question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
We have a GIF here attached to a JEOL 3010 and something strange has
happened to the energy selecting slit. It is far too wide (like a measured
width of about 70 eV, when nominally set to 1eV). Is there any user
intervention that can fix this problem easily? (e.g. Does anyone understand
how to use the Slit Width Calibration function in Filter Control, and would
this help?)

Or, is this simply a case for calling the Gatan Service Engineer?

Thanks in advance for any help you can give.

Best wishes

Ian MacLaren
N.B. New address
FB Materialwissenschaft - FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162893
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Wed May 15 05:48:00 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Wed, 15 May 2002 12:40:10 +0200
Subject: HREM TV Cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Has anybody out there installed a TV Camera for HREM imaging ABOVE the
normal viewing screen in a TEM?

We would like to install a TV Camera on a JEOL TEM for focussing HREM
images, but there's no space below the Camera due to other attachments being
there. Meanwhile, there are a couple of ports free above the viewing
screen.

If anyone has any ideas of good cameras / camera manufacturers for this
purpose, please contact me.

Thanks

Ian MacLaren
N.B. New address
FB Materialwissenschaft - FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162893
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Wed May 15 07:26:56 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Wed, 15 May 2002 08:17:06 -0400
Subject: ETEC micromanipulator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Previous and present ETEC owners,
Does anyone have an ETEC micromanipulator hidden away in a drawer or
cabinet? One of my customers has my only complete one and needs a
second for a project. Beg, borrow, rent or buy are all options. Thanks.

Ken Converse
owner
Quality Images
third party SEM service
16 Creek Rd.
Delta, PA 17314

717-456-5491




From daemon Wed May 15 07:46:10 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 15 May 2002 08:39:47 -0400
Subject: RE: Hot spot in epifluorescence

Contents Retrieved from Microscopy Listserver Archives
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Morning Eric,
Just a guess, but one of the things that is emitted in large
quantity is heat (ir?). In the old days when bright light in microscopy
always came from a carbon arc, the UV was filtered thru a 1"+ layer of
copper sulfate solution to cool off the light (remove the ir?).
Now, if I remember, most CCD detectors have a sweet spot in the near
or far red (ir?). My guess is that you may be 'seeing' with your CCD what
YOU cannot see during visual alignment.
I believe that there are barrier filters or N.D. filters in front of
the Hg lamp that will accomplish what you want.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Eric Johnston
} Sent: Tuesday, May 14, 2002 8:50 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Hot spot in epifluorescence
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi
}
} Long time lurker, first time poster (well, almost).
}
} We recently upgraded from a video camera to a cooled digital camera that
} is
} very sensitive. While doing epifluorescence on our Nikon TE300, we
} noticed
} some rather significant variations in the illumination from the mercury
} lamp, specifically a hot spot filling about 1/4 of the screen. This is
} not
} visible through the eyepiece and is not visible with the video camera. I
} have tried re-aligning the lamp in various ways. This moves the hot spot
} around but cannot make it go away. I even tried putting a diffuser in the
} light path. The hot spot is still there. I've had the vendors in, who
} say
} we might need a lamp with a bigger arc to fill the field of view.
}
} Does anybody have any suggestions?
}
} Eric
}
}
}


From daemon Wed May 15 08:05:43 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 15 May 2002 09:03:23 -0400
Subject: Ask-A-Microscopist: Detecting Ca in Al

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may want to try SIMS [Scanning Ion Mass Spectroscopy] [EDX may only be
able to do about 1,000 ppm, but that is sample dependent and matrix
dependent.]

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: giummc-at-rpi.edu [mailto:giummc-at-rpi.edu]
Sent: Tuesday, May 14, 2002 2:20 PM
To: Microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (giummc-at-rpi.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, May
14, 2002 at 12:37:15
---------------------------------------------------------------------------

Email: giummc-at-rpi.edu
Name: Cindie Giummarra

Organization: Rensselaer Polytechnic Institute

Education: Graduate College

Location: Troy, New York, USA

Question: Hi,

I was wondering which techniques I could use to detect calcium in
15-40ppm amounts in an aluminum alloy?

I am interested in finding out what form the Ca is in and where it is
located in the microstructure.

Thank you.

---------------------------------------------------------------------------


From daemon Wed May 15 08:46:32 2002



From: Isabel Nogueira :      isabeln-at-popsrv.ist.utl.pt
Date: Wed, 15 May 2002 14:39:04 +0100
Subject: EDS detector resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

We have an EDS detector attached to a 200 kV TEM that is having its crystal
changed (to a Si(Li)).
The original resolution was 139 eV. Now, depending on the amount of the
repair, it could become 138 or 143 eV.
My question is : is 143 eV worse than 138 eV in practise ? Does one notice
the difference ?

I appreciate any ideas.

Isabel


Isabel Nogueira
Lab Technicien
Instituto Superior Técnico
Materials Department
Avenida Rovisco Pais
1049-001 Lisboa
Portugal
tel.: +351 218418123
fax: +351 218418120
email: isabeln-at-popsrv.ist.utl.pt



From daemon Wed May 15 09:19:01 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 15 May 2002 09:11:42 -0500
Subject: Re: EDS detector resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suppose the issue depends on how you use your detector and which elements
you examine. If it is for elements up through arsenic, you should have
little trouble with overlaps and 143 eV resolution ought to be fine. If you
are looking at heavier metals with lots of overlaps, you may want to hold
out for the better resolution. For example, last week I was looking at a
Pb-Bi-Sn-Cd fusible alloy. That made for plenty of overlaps. Even so, I
think my results were good even without operating in the highest resolution
mode.

Our EDS offers 20, 10, and 5 eV/channel modes. Thus you are only talking
about a difference of 1/4th of a channel in the 20 eV/channel mode which we
use most of the time. I don't think you would notice it. But that is only
my guess.

Warren

At 02:39 PM 5/15/02 +0100, you wrote:

} Dear colleagues,
}
} We have an EDS detector attached to a 200 kV TEM that is having its crystal
} changed (to a Si(Li)).
} The original resolution was 139 eV. Now, depending on the amount of the
} repair, it could become 138 or 143 eV.
} My question is : is 143 eV worse than 138 eV in practise ? Does one notice
} the difference ?
}
} I appreciate any ideas.
}
} Isabel
}
} Isabel Nogueira
} Lab Technicien
} Instituto Superior Técnico
} Materials Department
} Avenida Rovisco Pais
} 1049-001 Lisboa
} Portugal

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Wed May 15 09:35:06 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 15 May 2002 07:27:43 -0700
Subject: Re: Biosafe adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} O.k. this is not mciroscopy directly BUT I hope someone out there
} has an
} answer. We're looking for an "adhesive" to glue #3 Whatman filter paper to
} stainless steel (surface area of ~ 3 sq cm or less) but it must be: (1)
} sterile or sterilizable, (2) water stable, (3) biologically non-toxic.
}
} Richard -

My son-in-law makes and repairs diamond scalpels (he started with diamond
EM knives, but decided that surgeons are less fussy & more clumsy than
microscopists). He, and the other manufacturers, cements the blades into
steel or titanium handles with a 2-part epoxy glue that can take autoclave
temperatures.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed May 15 09:42:14 2002



From: Debbie Lietz :      dlietz-at-trentu.ca
Date: Wed, 15 May 2002 10:35:03 -0400
Subject: Digital Cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have recently been thinking of purchasing the Nikpn Coolpix 995 for
digital photgraphy but I read some of the thread from last year
regarding attaching this camera to a microscope. I have attached it and
for what I was doing at the time it was suitable. Can anyone suggest
other digital cameras in the same price range that will do microscopy
work (compound and dissecting)(macro and micro), good outside and indoor
photos and can be used to photograph electrophoresis gels. Thanks
Debbie



From daemon Wed May 15 09:50:36 2002



From: msteglic-at-mail.mdanderson.org
Date: Wed, 15 May 2002 09:45:08 -0500
Subject: TEM samples - how long?

Contents Retrieved from Microscopy Listserver Archives
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Cathy

As stated previously, your average tech would not be a good thing. I have been
in a clinical lab for the past 18 years. When I first came to this lab it had 3
techs, myself included with no less then 8 years EM experience each. We were
processing around 1850 specimens per year with a turn around time of less than
48 hours (the specimens had been in glut for at least overnight). About half of
these were cut and viewed for diagnosis. This is an average of 7 processed per
day and 4 per day being cut and viewed. Some days were more some less. I
remember at least one day where we processed 15 specimens. So as you can see,
the question you should ask is not how many specimens can be processed, but how
many can be cut and scoped successfully in a day. Anyone that can follow a
protocol can process but only an experienced tech can successfully cut, stain,
scope, and print a specimen. If s/he is the only tech in the lab, then IMHO the
most one could expect would be maybe 4 or 5 specimens per day if s/he were doing
everything (the processing, cutting etc) and this would be pushing the limit to
do everyday day in and day out. If the specimens were in blocks, then one may be
able to do 8 or more in a day depending on the number of pictures taken and the
speed of the print processor in the darkroom, again not something I would want
to do every day. If your images are acquired digitally, then that would need to
be accounted for in the scheme of things.

Mannie Steglich
Tech Dir E M Lab
U T M D Anderson Cancer Center




From daemon Wed May 15 10:00:31 2002



From: Doug Cromey :      cromey-at-Arizona.edu
Date: Wed, 15 May 2002 07:55:21 -0700
Subject: Processing tissue for 3D reconstruction

Contents Retrieved from Microscopy Listserver Archives
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I'm trying to help a colleague refine their technique for a 3D
reconstruction project. They work with kidney. They are trying to
reconstruct all of the thin loops of Henle (approx 15um in diameter)
through a depth of about 3-4 mm. In a nutshell, their current protocol is:

* formaldehyde fix & process kidney tissue for TEM (w/o OsO4)
* embed in Spurrs (normal hardness)
* cut 1um sections with a glass knife, sampling every 5um
* etch the epoxy from the sections and perform immunohistochemistry
* capture images of the sections and software align them for 3D reconstruction

Their biggest complaint is aligning the sections. My thought was that this
might be as a result of compression due to sectioning (the block faces are
pretty large). Occasional wrinkles in the plastic don't help matters.

As we talked about the problem there were a couple of issues that I
raised. I'd appreciate feedback from the list, since I've not done any 3D
work before.

* would a harder formulation of Spurrs be a better choice?
* would a different epoxy altogether be a better choice?
* if a different epoxy, how about something like LRGold (apologies to vendors
of competing products), a methacrylate-type plastic that could be embedded
at a lower temp and might be better overall for their immuno-reactions (but
might be more prone to shrinkage and/or compression artifacts)?

Yours,
Doug Cromey

....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: Cromey-at-Arizona.edu) :
:...................................................................:
http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Wed May 15 10:17:34 2002



From: Eric Johnston :      ericdj-at-seas.upenn.edu
Date: Wed, 15 May 2002 11:09:27 -0400
Subject: Re: Hot spot in epifluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

wow, I got a lot of really useful replies.

The majority suggest that there is some kind of reflection on one or more
of the lens surfaces that's causing the hot spot, especially from the
camera adapter (in my case, the adapter has no lens so that's out).

The other suggestion was that the hot spot is due to IR, which CCDs can be
especially sensitive to. I need to find out if there is an IR blocker in
light path or on the camera itself.

Thanks for all the helpful suggestions. I'll keep you updated.

Eric


From daemon Wed May 15 10:55:40 2002



From: Taylor H. Ricketts :      ricketts-at-leland.stanford.edu
Date: Wed, 15 May 2002 08:48:01 -0700
Subject: Fwd: Wild scope repair/service

Contents Retrieved from Microscopy Listserver Archives
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} I have a relatively old Wild dissecting scope that needs
} repair/service. Does anyone know a good place to have that done in the
} Bay area, preferably near Stanford? Is this the kind of thing that
} Universities typically have standing contracts with someone for?

thanks,

Taylor




From daemon Wed May 15 12:40:34 2002



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Wed, 15 May 2002 10:31:37 -0700
Subject: RE: EDS detector resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Maybe more important than the quoted detector resolution is how you choose
to run the EDS system. Using small amplifier time constants and high
counting high rates can degrade the actual resolution a lot more than just a
few eV.

For historic reasons, EDS detector resolution is quoted at the energy of Mn
K-alpha x-rays (5.4 keV). However, the EDS resolution is actually has
energy dependent and energy independent contributions, so the x-ray peaks at
O-K, for example will be much narrower than those at Mn or Fe-K. Degrading
the detector resolution by adding (energy independent) electronic noise will
broaden the low-energy peaks much more than it does the high-energy ones.

Warren's comment about heavy elements and peak overlaps might need
clarification. For EDS of heavy elements in a TEM (as opposed to an SEM),
you can use the K or L-shell x rays with energies up to 20, 40 keV, or even
higher energies to avoid significant overlaps. The problem with peak
overlaps arises when you want to include light elements in the analyses and
the K-peaks from those elements overlap with higher-shell x-rays (e.g., L
or M) from the heavy elements in your sample. In that case, you might want
better resolution.

Larry

----------
From: Warren E Straszheim
Sent: Wednesday, May 15, 2002 7:11 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: Re: EDS detector resolution


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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-----------------------------------------------------------------------.


I suppose the issue depends on how you use your detector and which
elements
you examine. If it is for elements up through arsenic, you should
have
little trouble with overlaps and 143 eV resolution ought to be fine.
If you
are looking at heavier metals with lots of overlaps, you may want to
hold
out for the better resolution. For example, last week I was looking
at a
Pb-Bi-Sn-Cd fusible alloy. That made for plenty of overlaps. Even
so, I
think my results were good even without operating in the highest
resolution
mode.

Our EDS offers 20, 10, and 5 eV/channel modes. Thus you are only
talking
about a difference of 1/4th of a channel in the 20 eV/channel mode
which we
use most of the time. I don't think you would notice it. But that is
only
my guess.

Warren

At 02:39 PM 5/15/02 +0100, you wrote:

} Dear colleagues,
}
} We have an EDS detector attached to a 200 kV TEM that is having its
crystal
} changed (to a Si(Li)).
} The original resolution was 139 eV. Now, depending on the amount of
the
} repair, it could become 138 or 143 eV.
} My question is : is 143 eV worse than 138 eV in practise ? Does one
notice
} the difference ?
}
} I appreciate any ideas.
}
} Isabel
}
} Isabel Nogueira
} Lab Technicien
} Instituto Superior Técnico
} Materials Department
} Avenida Rovisco Pais
} 1049-001 Lisboa
} Portugal

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of
materials
Computer applications and networking







From daemon Wed May 15 12:45:00 2002



From: gerard.d.gagne-at-abbott.com
Date: Wed, 15 May 2002 12:37:02 -0500
Subject: Re: Biosafe adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} O.k. this is not mciroscopy directly BUT I hope someone out there
} has an
} answer. We're looking for an "adhesive" to glue #3 Whatman filter paper to
} stainless steel (surface area of ~ 3 sq cm or less) but it must be: (1)
} sterile or sterilizable, (2) water stable, (3) biologically non-toxic.
}
} Richard -

My son-in-law makes and repairs diamond scalpels (he started with diamond
EM knives, but decided that surgeons are less fussy & more clumsy than
microscopists). He, and the other manufacturers, cements the blades into
steel or titanium handles with a 2-part epoxy glue that can take autoclave
temperatures.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html






From daemon Wed May 15 14:59:01 2002



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu (by way of
Date: Wed, 15 May 2002 14:48:00 -0500
Subject: Glow discharge unit

Contents Retrieved from Microscopy Listserver Archives
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Hello,
Our glow discharge unit is becoming unreliable, and we are thinking about
replacing it. We have contemplated having a dedicated stand alone unit versus
going with a large vacuum evaporator multi-function system. I send this
message out so that vendors can respond directly to me, and also as
a solicitation for what
technology has/hasn't worked for others.
Thanks in advance,
Randy Nessler
University of Iowa
Central Microscopy Research Facility
Iowa City, Iowa 52358
Phone 319-335-8142
Fax 319-384-4469


From daemon Wed May 15 17:38:25 2002



From: Alan E. Davis :      adavis-at-saipan.com
Date: Thu, 16 May 2002 08:26:32 +1000
Subject: More digiterata: Microscope attachment for Olympus E20N?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just another posting about digital microscope adaptors. I am looking for informatio leading to the ordering and purchase of an adaptor for the Olympus E20N digital camera.

At the college lab where I will be teaching as an adjunct this summer, a new toy has been purchased: an Olympus E20N digital camera. Some discussion has focused on purchase of a microscope attachment. The attachment that has been recommended by one of the staff attaches to the 62mm filter ring threads of the camera. I have two scopes that I use in teaching, each of them w/ c. 45. eyetube inclination angles; there is no trinocular tube available. I fear the forces of attaching a camera to a scope by the filter ring at such an angle would be unhealthy for the camera. The camera is a hefty one.

Alan Davis

--
Alan E. Davis
Science Department
Marianas High School
PMB 30, Box 10006,
Saipan, MP 96950
Northern Mariana Islands
adavis-at-saipan.com


"An inviscid theory of flow renders the screw useless, but the need
for one non-existent."
---Lord Raleigh(aka John William Strutt),or else
his son, Jr., who was also a scientist.


From daemon Wed May 15 17:51:34 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 15 May 2002 15:46:50 -0700
Subject: Re: Digital Cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Debbie,
I recently saw a new Sony camera with a large lens that can focus down to
about one inch and claims to have better low-light performance than most
digitals. It is a 5 megapixel and sells for about what the Nikon 995 did
when it was first introduced (~$1650.00CAN). I'm sorry, I don't know the
model number, but it is hard to miss, since it looks funny with a tiny
digital camera body attached to a large, complex optical lens. It might best
suit your multiple needs.
At 10:35 AM 5/15/02 -0400, you wrote:

} I have recently been thinking of purchasing the Nikpn Coolpix 995 for
} digital photgraphy but I read some of the thread from last year
} regarding attaching this camera to a microscope. I have attached it and
} for what I was doing at the time it was suitable. Can anyone suggest
} other digital cameras in the same price range that will do microscopy
} work (compound and dissecting)(macro and micro), good outside and indoor
} photos and can be used to photograph electrophoresis gels. Thanks
} Debbie
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Wed May 15 18:08:04 2002



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Wed, 15 May 2002 16:01:51 -0700
Subject: Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Engineer Senior/Principal

Arizona State University Center for Solid State Science is seeking
qualified candidates to fill the position Engineer Senior or Principal.
Level and salary will be determined based on qualifications. This is a
full time, benefit eligible position. This position is responsible for
maintaining high levels of performance in analytical high-resolution
electron microscopes particularly electrical, magnetic and mechanical
stability, while concurrently developing these systems to achieve higher
levels of performance, including computer interfacing.

Complete qualifications and application information please refer to SR
108236 at www.asu.edu/hr/jobs/. Application deadline is May 28, 2002 and
every two weeks thereafter until search is closed.


John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Thu May 16 01:07:34 2002



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Thu, 16 May 2002 07:59:03 +0200
Subject: Re: Hot spot in epifluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I am not at my office at the moment (using webmail) so I cannot check directly
but I have some filters from way back which I think I bought from Schott (I
have no connection with the company!).

Try going to

www.google.com

and searching on

optical glass filters

or something similar. Schott comes up as do many others of course.

Good luck

Gareth

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Thu May 16 03:05:29 2002



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Thu, 16 May 2002 19:50:00 +1000
Subject: Re: Digital Cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 15, 2002 2:23 AM


I have seen today Panasonic Lumix DMC-LC5A with Leica lenses and looks
also very good.
3,9 Mega pixels
just for AUS dollars 1775
Ricardo
Keep care and be of good cheer

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
(title) 84 duke of Siebenlügner

websites:
http://www.coleoptera.org. and
http://www.egroups.com/group/coleoptera

University of Sydney
The Wentworth Bldg., B 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ICQ: 13610107

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).



From daemon Thu May 16 07:51:42 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 16 May 2002 08:42:34 -0400
Subject: RE: More digiterata: Microscope attachment for Olympus E20N?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Alan,
At 100g without batteries, I would certainly NOT mount such a camera
without the added support of a mini-tripod, or macro-stand.

Here are some sites that have adapters:

http://www.marksimmons.org/closeup/adapter/adapter.html

http://www.marksimmons.org/closeup/adapter/adapter.html [for angle
viewing???]

http://www.mir.com.my/rb/photography/hardwares/classics/olympusom1n2/om1/om1
manual/index5.htm
[an old Olympus OM1 site with a 'massive' camera stand to isolate it
from scope]

http://www.lensadapter.com/optical_article.htm [here's a guy who has your
problem]
http://www.lensadapter.com/default.htm [looks like just what you need!!!!]

These last two were the best finds of the exercise. Hope it helps.

Fred



} ----------
} From: Alan E. Davis
} Sent: Wednesday, May 15, 2002 6:26 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: More digiterata: Microscope attachment for Olympus E20N?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Just another posting about digital microscope adaptors. I am looking for
} informatio leading to the ordering and purchase of an adaptor for the
} Olympus E20N digital camera.
}
} At the college lab where I will be teaching as an adjunct this summer, a
} new toy has been purchased: an Olympus E20N digital camera. Some
} discussion has focused on purchase of a microscope attachment. The
} attachment that has been recommended by one of the staff attaches to the
} 62mm filter ring threads of the camera. I have two scopes that I use in
} teaching, each of them w/ c. 45. eyetube inclination angles; there is no
} trinocular tube available. I fear the forces of attaching a camera to a
} scope by the filter ring at such an angle would be unhealthy for the
} camera. The camera is a hefty one.
}
} Alan Davis
}
} --
} Alan E. Davis
} Science Department
} Marianas High School
} PMB 30, Box 10006,
} Saipan, MP 96950
} Northern Mariana Islands
} adavis-at-saipan.com
}
}
} "An inviscid theory of flow renders the screw useless, but the need
} for one non-existent."
} ---Lord Raleigh(aka John William Strutt),or else
} his son, Jr., who was also a scientist.
}
}


From daemon Thu May 16 12:21:57 2002



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Thu, 16 May 2002 13:13:05 -0400
Subject: TEM position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Army Research Laboratory, Adelphi MD (http://www.arl.army.mil) is about
to advertise a position for an experienced Ph.D. level TEM specialist. We
are looking for someone with experience and publications in the TEM of
semiconductor materials/devices. Experience with or interest in Auger
electron spectroscopy, MOCVD, wide bandgap materials, and FIB sample
preparation would be beneficial. We have a JEOL 2010 and a JEOL 2010F with
EDX. Applicants MUST BE US CITIZENS. ARL is an equal opportunity
employer. Please e-mail resumes directly to me, although, this will not
constitute an official application for the position which must be done by
responding to the official posting.

Sincerely,
Matthew Ervin, Ph.D.
MErvin-at-ARL.Army.mil

M/S: AMSRL-SE-RL
US Army Research Laboratory
2800 Powder Mill Road
Adelphi, MD 20783-1197

Disclaimer: The opinions and views expressed above are those of the author
and do not necessarily represent those of the U.S. Army Research Laboratory
or any other government agency



From daemon Thu May 16 13:26:25 2002



From: George Laing :      scisales-at-ngscorp.com
Date: Thu, 16 May 2002 14:18:05 -0400
Subject: RE: Digital Cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The camera you refer to is the Sony DSC-F707. It has 5MP resolution, a
Zeiss zoom lens, USB interface and uses Memory Stick media for storage.
http://www.sonystyle.com/digitalimaging/P_Feature_F707.shtml

} I recently saw a new Sony camera with a large lens that can focus } down to
about one inch and claims to have better low-light
} performance than most digitals.... ... it is hard to miss, since } it looks
funny with a tiny digital camera body attached to a
} large, complex optical lens.

The Nikon Coolpix 5000 is also a 5MP camera with a Nikon zoom lens, .75
inch minimum focus, and accepts compact flash media. It will also shoot
320x240 Quicktime movies(the Sony also has a "video" mode). Image quality
is excellent. In addition to standard exposure controls, the CP5000 also
has adjustments for contrast, noise reduction, sharpening and saturation.
An electronic release is also available to reduce potential vibration from
depressing the shutter or to do interval timing. Microscope couplers are
readily available for the 5000 to fit most microscopes.
http://www.nikonusa.com/usa_product/product.jsp?cat=1&grp=2&productNr=25501
or we have a PDF available.

} Can anyone suggest other digital cameras in the same price range } that
will do microscopy work (compound and dissecting)(macro and
} micro), good outside and indoor photos and can be used to photograph
electrophoresis gels.

George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com




From daemon Thu May 16 14:52:56 2002



From: Sarka Lhotak :      lhotaks-at-mcmail.cis.mcmaster.ca
Date: Thu, 16 May 2002 15:44:31 -0400 (EDT)
Subject: Retiga 1300 camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
would anybody have any thoughts on the Retiga 1300 Monochromatic
cooled CCD camera (for fluorescent microscopy) a and its price: CAN$
12,300.00 (CAN$ = US$ 0.63).

Thank you for your help,
Sincerely,

Sarka Lhotak

Hamilton Regional Cancer Centre
McMaster University
Hamilton, Ontario, Canada



From daemon Thu May 16 15:18:12 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 16 May 2002 16:11:47 -0400
Subject: Problem with CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
We have a EMITech K850 Critical Point Dryer and a new tank of
Research Grade CO2, and we have problems.
The runs have all of a sudden become VERY inconsistent.

Ambient temp in the Lab is around 18oC
After cooling the chamber to 5oC and opening the Inlet valve, the
pressure rises to 750psi at which is will remain for at least an hour with
all valves closed.
With the Inlet valve open, however, and waiting for liquid CO2 to
show in the sight glass, the temperature begins to rise and does so to
around 10oC at which point the fluid begins to rise in the glass to about
1/8 of the 1cm diameter.
If I re-cool the chamber to 5oC, the fluid disappears.
If I wait for up to 3 minutes, one of two things happens.
1. the fluid reappears and rises to 1/2 the sight window,
or
2. I see nothing until I release the pressure and for a
brief period note the fluid level show at the very top of the sight glass
and fall and disappear very rapidly.
After the first run, which I cannot control, I cannot merely recyle
the unit. I have to wait an hour to demonstrate another unsatisfactory
test.

If anyone can help me ferret out this poltergeist, I would be most
appreciative. I must say that I am convinced that I am dealing with a
daemon from the PChem lab.

I attached my Polaron CPD, which is much simpler in design and saw
the same initial 750 psi but NO fluid accumulation after watching for 10min.

Hoping that it is NOT that terrible tank with the broken siphon (again after
all these years),

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Fri May 17 02:40:14 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 17 May 2002 09:30:28 +0200
Subject: Re: More digiterata: Microscope attachment for Olympus E20N?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Look at :

http://www.microscopy-uk.org.uk/mag/artaug01/vrcoolpix.html

It's a nice description of a home made adaptor to fit a Nikon CoolPix on a
microscope, using the tripod thread insted of the filter ring threads of
the camera. I don't know if the Olympus E20N has such a thread, but it
gives some idee how to build something which works well and is not
expensive.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Thu, 16 May 2002, Alan E. Davis wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Just another posting about digital microscope adaptors. I am looking
} for informatio leading to the ordering and purchase of an adaptor for
} the Olympus E20N digital camera.
}
} At the college lab where I will be teaching as an adjunct this summer,
} a new toy has been purchased: an Olympus E20N digital camera. Some
} discussion has focused on purchase of a microscope attachment. The
} attachment that has been recommended by one of the staff attaches to
} the 62mm filter ring threads of the camera. I have two scopes that I
} use in teaching, each of them w/ c. 45. eyetube inclination angles;
} there is no trinocular tube available. I fear the forces of attaching
} a camera to a scope by the filter ring at such an angle would be
} unhealthy for the camera. The camera is a hefty one.
}
} Alan Davis
}
} --
} Alan E. Davis
} Science Department
} Marianas High School
} PMB 30, Box 10006,
} Saipan, MP 96950
} Northern Mariana Islands
} adavis-at-saipan.com
}
}
} "An inviscid theory of flow renders the screw useless, but the need
} for one non-existent."
} ---Lord Raleigh(aka John William Strutt),or else
} his son, Jr., who was also a scientist.
}



From daemon Fri May 17 09:07:20 2002



From: Kevran44-at-aol.com
Date: Fri, 17 May 2002 09:24:23 EDT
Subject: Re. Detector Resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't think you will see the difference between 143 and 138 resolution
there are many factors that come into play as previously mentioned. Another
one is are you replacing the window on your detector also. There are an
assortment of different windows that have different energy line curves and
strengths. Some may be more suited to your application that is if you are
doing something so touchy that 138 resolution to 143 resolution is going to
come into play. Why did you need to replace your crystal? Are you replacing
your F. E. T. also, as that will effect the detector resolution as well.

Daniel Connors


From daemon Fri May 17 10:21:30 2002



From: Peter Guthrie :      Peter.Guthrie-at-hsc.utah.edu
Date: Fri, 17 May 2002 09:01:00 -0600
Subject: ISP Question (USA) - no Microscopy content

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry for intruding with a non-microscopy question, but I am looking
for feedback on a nationwide ISP. My present ISP has dropped their
East Coast dial-ups, and I need access when I travel.

I am looking for the least-intrusive (i.e. not AOL) nationwide ISP.
Any suggestions?

Please reply privately unless you have some information you would
like the entire list to see.

Thanks

pbg


From daemon Fri May 17 11:05:02 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 17 May 2002 11:58:19 -0400
Subject: CPD Question Two

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,


For those who order CO2-w/siphon tube for critical point drying.

Do you order:

the 'Research Grade' CO2 (-at-$300) or

the standard grade CO2 (-at-$7)?

Thanks,

Fred Monson


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Fri May 17 11:45:23 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 17 May 2002 09:38:09 -0700
Subject: RE: Digital Cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Sony F707, Nikon 5000, Olympus E20N and Minolta Dimage 7 all use the
same 5 Mega Pixel Sony CCD. Reviews, and test photos comparing resolution,
color response and S/N characteristics can be found at this web site:
http://www.dpreview.com/
The way I read it, the Sony F707 beats them all quite handily. In addition,
the lens is internal focusing, so the end of the camera doesn't move during
focusing or poweron/poweroff. In addition, the body with the display on it
tilts so that it can be viewed conveniently while mounted vertically. This
would be a good to adapt to a microscope, but I do not know if anyone has
built a commercially available adapter.

John Mardinly




-----Original Message-----
} From: George Laing [mailto:scisales-at-ngscorp.com]
Sent: Thursday, May 16, 2002 11:18 AM
To: MSA Microscopy Society of America


The camera you refer to is the Sony DSC-F707. It has 5MP
resolution, a
Zeiss zoom lens, USB interface and uses Memory Stick media for storage.
http://www.sonystyle.com/digitalimaging/P_Feature_F707.shtml

} I recently saw a new Sony camera with a large lens that can focus } down to
about one inch and claims to have better low-light
} performance than most digitals.... ... it is hard to miss, since } it looks
funny with a tiny digital camera body attached to a
} large, complex optical lens.

The Nikon Coolpix 5000 is also a 5MP camera with a Nikon zoom lens,
.75
inch minimum focus, and accepts compact flash media. It will also shoot
320x240 Quicktime movies(the Sony also has a "video" mode). Image quality
is excellent. In addition to standard exposure controls, the CP5000 also
has adjustments for contrast, noise reduction, sharpening and saturation.
An electronic release is also available to reduce potential vibration from
depressing the shutter or to do interval timing. Microscope couplers are
readily available for the 5000 to fit most microscopes.
http://www.nikonusa.com/usa_product/product.jsp?cat=1&grp=2&productNr=25501
or we have a PDF available.

} Can anyone suggest other digital cameras in the same price range } that
will do microscopy work (compound and dissecting)(macro and
} micro), good outside and indoor photos and can be used to photograph
electrophoresis gels.

George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com




From daemon Fri May 17 15:26:12 2002



From: Frank Eugene Jones :      jonesfe-at-darkwing.uoregon.edu
Date: Fri, 17 May 2002 13:17:18 -0700 (PDT)
Subject: remote connection to Oxford Lemas stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

I am trying to interface a PC to an Oxford eXL/Lemas system on a
JEOL 6300 SEM in order to get external control of the stage. Has anyone
tried this and/or do you have any suggestions for getting this to work?

Connections:

(1) eXL (main Oxford control computer using Genie OS) {-{6 pin
"LaserBus" & 15 pin stage sync.}-} Lemas (stage motherboard and motor
drivers)

(2) Lemas {-{15 pin cable}-} joystick & keypad

(3) Lemas {-} stepper motors & limit

Thanks!
*************************************************************************
Frank E. Jones email: jonesfe-at-darkwing.uoregon.edu
Department of Physics URL: http://darkwing.uoregon.edu/~jonesfe
Office: 189b Klamath
Lonergan Lab (Chemistry)
University of Oregon
Office Phone: (541)346-0977
-------------------------------------------------------------------------





From daemon Fri May 17 21:29:46 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 17 May 2002 22:24:49 -0400
Subject: Re: Hot spot in epifluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric & Co.;

The I.R., in my opinion, seems the likely suspect for the hotspot. I took
an optical microscope and deliberately converted it into an IR.. reflected
light microscope to image through crystalline compounds like GaAs. What I
did was put a filter in the path that blocked the visible light but was a
bandpass to I.R. The I.R. naturally is generated by the illuminator. Of
course the human eye cannot see into the infra-red but a CCD camera can and
does and that is used for imaging and recording vis-a-vis a monitor. I
removed the I.R. blocking filter from a B/W camera [just in front of the CCD
array to make this work] I also have what you described as a "hot spot." I
am trying to diffuse this with a filter that will still transmit I.R. In
your case you may need an I.R. blocking filter as you stated. I am looking
at Edmund Scientific for a low budget solution.

Please let us know if, or when, you find a solution.

Regards,

Peter Tomic
Anadigics, Inc.



-----Original Message-----
} From: Eric Johnston [mailto:ericdj-at-seas.upenn.edu]
Sent: Wednesday, May 15, 2002 11:09 AM
To: Microscopy-at-sparc5.microscopy.com


Hi all

wow, I got a lot of really useful replies.

The majority suggest that there is some kind of reflection on one or more
of the lens surfaces that's causing the hot spot, especially from the
camera adapter (in my case, the adapter has no lens so that's out).

The other suggestion was that the hot spot is due to IR, which CCDs can be
especially sensitive to. I need to find out if there is an IR blocker in
light path or on the camera itself.

Thanks for all the helpful suggestions. I'll keep you updated.

Eric


From daemon Sat May 18 10:39:57 2002



From: Gordon Nord :      gnord-at-mindspring.com
Date: Sat, 18 May 2002 11:26:16 -0400
Subject: Inexpensive recirculating chiller for diffusion pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,

I have a dual gun Gatan ion thinner that runs a 1500W oil diffusion pump.

The rules at Brooklyn College require no heat down the drain and water
conservation.
Thus I need a recirculating chiller. Current lab chillers are $4,000 and
up
however the pump doesn't require constant temperature so I don't see why
an expensive controller, etc is necessary.

Any ideas for a recirculating chiller that is less expensive?

Also any US government labs with a surplus recirculator. It can be
donated to an American University.

Thanks,
Gordon Nord
Environmental Science Institute
Brooklyn College, Brooklyn, NY
gnord-at-mindspring.com



From daemon Sat May 18 14:11:58 2002



From: John W. Raffensperger, Jr. :      johnr-at-helwigcp.com
Date: Sat, 18 May 2002 14:05:08 -0500
Subject: Amateur Microscopy Associations

Contents Retrieved from Microscopy Listserver Archives
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I hope you don't mind my intrusion onto your list sever. It was
suggested
by the business office that I post my question here.

I am an amateur microscopist that has dabbling on and off since grade
school,
exploring the world of the miniature, and interesting views of the
ordinary.

I have recently become more serious about this "hobby", and have adapted
a
digital camera to the photo tube of an optical microscope.

I am hoping to find some type of amateur microscopy association(s) that
would
help enhance my work and enjoyment. My web search only turned up a
group in
the UK, and I was hoping to find something "closer to home" (Wisconsin,
USA).

Thank you kindly;

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.




From daemon Sat May 18 17:43:04 2002



From: kchamusco-at-mail.ifas.ufl.edu ()
Date: Sat, 18 May 2002 20:56:15 -0500
Subject: Ask-A-Microscopist: corn pollen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,
I prefer the Neslab CFT 25 chiller, either digital or analog. The work
just fine for a small DP and are very reliable. Check out LABx or a
reseller like Bid Service. They usually have some in stock, around $1K.

Gary M. Easton
Scanners Corporation
Third Party SEM Service

No financial interest in any of the above companies(except mine of course).

----- Original Message -----
} From: "Gordon Nord" {gnord-at-mindspring.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, May 18, 2002 11:26 AM


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kchamusco-at-mail.ifas.ufl.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
May 18, 2002 at 16:01:58
---------------------------------------------------------------------------

Email: kchamusco-at-mail.ifas.ufl.edu
Name: karen chamusco

Organization: U of FL

Education: Graduate College

Location: Gainesville FL

Question: I'm sorry if I should be usinf the list serve but this is
the only page I could find to ask a question. I have been working
with corn pollen and trying to embed it in LR White resin for
immunolabeling in TEM. I thought I had this problem solved, but,
apparently I don't. When I get my pollen through the EtOH series up
to 95% it looks very good, but when I try to embed it in the resin it
collapses. I have tried putting it in resin/EtOH at 20% steps and
directly embedding it in 100% resin. The 20% stepwise series seems to
be the least destructive and I can get partially collapsed pollen
sometimes. I really need to be able to see inside a "fluffy" whole,
uncollapsed pollen grain. Is there anyone who can help with this
before I pull out the rest of my hair? If necessary to know, I fix
in 4% paraformaldehyde + 0.5% gluteraldehyde + 4% sucrose + 0.3%
tween {to get the samples to sink}. I vacuum infiltrate for 5 minutes
then I leave them in the fridge overnight. I then rinse with PBS and
send through a 10% EtOH series{anything greater causes the pollen to
collapse}. Then the rest is as I described above. Thanks, Karen

---------------------------------------------------------------------------


From daemon Sun May 19 02:29:37 2002



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 19 May 2002 02:20:46 -0500
Subject: Re: Hot spot in epifluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you use a CCD or CMOS camera you need an IR filter some where. Most color
cameras have some filtering but not all. Many monochrome cameras do not have
any filtering.

IR light will be out of focus as well as causing hot spots. Silicone in a
CCD camera is sensitive into IR light to 1000 nm. With the sensitivity at
800 nm being high enough to cause some problems. A curve with and with out
IR blocking filters are given here:
http://www.edmundoptics.com/techsupport/DisplayArticle.cfm?articleid=266

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger
} From: "Peter Tomic" {PTomic-at-anadigics.com}
: Eric & Co.;
:
: The I.R., in my opinion, seems the likely suspect for the hotspot. I took
: an optical microscope and deliberately converted it into an IR.. reflected
: light microscope to image through crystalline compounds like GaAs. What I
: did was put a filter in the path that blocked the visible light but was a
: bandpass to I.R. The I.R. naturally is generated by the illuminator. Of
: course the human eye cannot see into the infra-red but a CCD camera can
and
: does and that is used for imaging and recording vis-a-vis a monitor. I
: removed the I.R. blocking filter from a B/W camera [just in front of the
CCD
: array to make this work] I also have what you described as a "hot spot."
I
: am trying to diffuse this with a filter that will still transmit I.R. In
: your case you may need an I.R. blocking filter as you stated. I am
looking
: at Edmund Scientific for a low budget solution.
:
: Please let us know if, or when, you find a solution.
:
: Regards,
:
: Peter Tomic
: Anadigics, Inc.
:
:
:
: } From: Eric Johnston [mailto:ericdj-at-seas.upenn.edu]
: :
: wow, I got a lot of really useful replies.
:
: The majority suggest that there is some kind of reflection on one or more
: of the lens surfaces that's causing the hot spot, especially from the
: camera adapter (in my case, the adapter has no lens so that's out).
:
: The other suggestion was that the hot spot is due to IR, which CCDs can be
: especially sensitive to. I need to find out if there is an IR blocker in
: light path or on the camera itself.
:
: Thanks for all the helpful suggestions. I'll keep you updated.
:
: Eric
:
:




From daemon Sun May 19 06:17:49 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Sun, 19 May 2002 12:11:23 +0100
Subject: Chillers on the roof

Contents Retrieved from Microscopy Listserver Archives
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In about 6 months time I will have to move my microscopes into a newly
re-furbished two-storey building. The chillers for the FEI CM120 and
Hitachi 4700 may have to be located on the roof, at about 8.5 metres
from ground level. I would like to hear from anyone who has
considered this, and would particularly value the first-hand
experiences of anyone who has already tried it. Are the pumps in
standard chillers capable of dealing with an 8-metre head?

Chris



From daemon Sun May 19 20:02:40 2002



From: Beauregard :      beaurega-at-westol.com
Date: Sun, 19 May 2002 19:55:04 -0400
Subject: Re: Chillers on the roof

Contents Retrieved from Microscopy Listserver Archives
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Hi Chris,

We had a Nesslab unit with a pump in our 4th floor fan loft.
Our CM12 and SEM were located on the first floor.

I installed pressure gauges at the inlet and outlet lines where they enter
the labs. Typical readings on the first floor were 55 PSI on the inlet
(from Nesslab) and 15 PSI on the return outlet after going through our
scopes. This 15 psi 'back pressure' was simply the pressure of the water
standing in the pipe that was 3-4 floors or about 30-35 feet high.
The first floor inlet pressure was then 40 PSI + 15 PSI or 55 PSI to the
scopes. This is only a net pressure difference of 40 PSI across your scopes.

Upstairs on the outlet side of the Neslab, the pressure gauge at the unit
in the fan loft was 40 psi. The 'suction' or inlet at the Nesslab read
zero. The buffer tank is open to air. YOUR chiller will not even notice
any pressure difference but you will get a loss of 15 PSI to your
instruments as shown above.

The net effect was that we had an extra 15 PSI on the whole system on the
first floor even when the pump was turned OFF. Don't remove any lines on
instruments without shutting both the return and the supply lines OFF.

This pressure worked out to 5 PSI per floor for the three floors above us.
I think you will see about 15 PSI back pressure on your instruments on the
first floor.

We were forced to install a system that runs off our HVAC chilled water
through two heat exchangers in series. It has automatic city water
chilling for backup. The temperature of city water is not constant
throughout the year.
The last heat exchanger and pump is on the first floor and eliminates the
back pressure problem. You might consider a heat exchanger and pump on
your floor to eliminate the back preesure. My CM 12 can handle much higher
pressures. Your does too I believe.

As for your problems:
Run DI or distilled water on a CM series scope. Never use chloramine-T
because of chloride pit corrosion of SS fittings. Check with Philips
service.

Make sure you have a DI water pump to keep the recirc' cooling tank topped
off. If you have a leak on the first floor, then you will empty any open
buffer tank 'upstairs' fast.

We had a small pump tied into a big DI water tank to deliver enough water
to buy us some time to react to a leak problem. It automatically topped
off the buffer tank. Also if I drained any water out on the first floor to
change the water, it pumped in the required replacement amount without
going upstairs.

You need someone in the group to go around and check that all plastic
connections are water tight and secure to avoid draining the buffer tank
over a weekend, say. Use brass tubing inserts and hose clamps. Make sure
all plastic solvent welds are secure.

Use good rubber pressure hose where possible.

You might need a remote temperature light and readout in the first floor
hallway to tell someone when there is a problem. This wasn't that
effective an alarm system for us. People just walked by without noticing a
problem. SO.......

I also have in my lab a small cheap audible temp alarm that has the probe
fitted under the chilled water insulation. It is set to go off if any
minute temperature rise occurs. I bought this from McMaster-Carr Hardware
supply. If it even chirps, I go check the system light and temp readout
down the hall, check all pressure readings, and go up to the fan loft and
watch the freon compressor cycle. This alarm can also alert you to a
partial loss in freon before a sudden total system failure and it allows
one to shut down all instruments safely and to notify maintainence of a
slight loss in cooling. I highly recommend this alarm.

We originally installed L-type copper 1 inch manifold lines from the fan
loft to the first floor and the labs. We tore all that out and now use
CPVC dark gray plastic pipe, I believe. This was done to avoid green
copper carbonate build up in the RETURN--RETURN lines after diffusion pumps.

Hope this helps.

Paul Beauregard
Senior Research Associate

}
} In about 6 months time I will have to move my microscopes into a newly
} re-furbished two-storey building. The chillers for the FEI CM120 and
} Hitachi 4700 may have to be located on the roof, at about 8.5 metres
} from ground level. I would like to hear from anyone who has
} considered this, and would particularly value the first-hand
} experiences of anyone who has already tried it. Are the pumps in
} standard chillers capable of dealing with an 8-metre head?
}
} Chris
}
}
}
}



From daemon Mon May 20 07:25:53 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 20 May 2002 08:15:04 -0400
Subject: RE: CPD Question Two-Summary

Contents Retrieved from Microscopy Listserver Archives
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Morning All,
For those who are interested in the responses to my last question,
here's the dope.

I received 9 responses from VERY reputable sources.
NONE used what I called 'Research Grade' CO2
NONE paid more than ~$40, and most paid {$20 / tank
Most ordered what they called "bone dry" CO2
One used a hot water pipe heating strip for 1hr before use
to insure access to entire liquid charge in tank.

Appreciation to all for your help and advice.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Monson, Frederick C.
} Sent: Friday, May 17, 2002 11:58 AM
} To: 'List-Microscopy'
} Subject: CPD Question Two
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
}
}
} For those who order CO2-w/siphon tube for critical point drying.
}
} Do you order:
}
} the 'Research Grade' CO2 (-at-$300) or
}
} the standard grade CO2 (-at-$7)?
}
} Thanks,
}
} Fred Monson
}
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} West Chester University
} South Church Street and Rosedale
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}


From daemon Mon May 20 08:33:07 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 20 May 2002 09:20:42 -0400
Subject: Re: CPD Question Two

Contents Retrieved from Microscopy Listserver Archives
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At 11:58 AM -0400 5/17/02, Monson, Frederick C. wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Mon May 20 10:27:15 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Mon, 20 May 2002 11:17:30 -0400
Subject: Re: Problem with CPD

Contents Retrieved from Microscopy Listserver Archives
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Sounds like you have a flow rate problem something is constricting the
flow of CO2 into the chamber. The chamber will get warm when you fill it (the
CO2 in the syphon tank is at 18 C vs the chamber at 5 C). When you cool
the chamber the CPD uses vented high pressure CO2 to cool the chamber
(PV=nRT), but since there is a restriction in the flow from the CO2 supply
tank, the CO2 back flows from the chamber dropping the CO2 liquid level.

I have found CO2 flow problems caused by:

- low CO2 supply tank

- tank valve must be openned fully (minus one 1/2 turn) - don't use the
tank valve as a regulator.

- clogged filters. I have worked with different setups with various line
filters (particle, oil, moisture, etc.) which get clogged and need replacing. I
have also found some CPDs have (scintered?) metal mesh filters for particle
filtration and these get clogged too.

- Worked with one instrument in which the internal plumbing lines were
just too small and if you flushed or cooled too fast the liquid level in the
chamber would drop and you had to be very carful and slow about things.

Hope it helps!

(Oh we use the cheap standard CO2)

On 16 May 2002, at 16:11, Monson, Frederick C. wrote:


}
} Hi All,
} We have a EMITech K850 Critical Point Dryer and a new tank of
} Research Grade CO2, and we have problems.
} The runs have all of a sudden become VERY inconsistent.
}
} Ambient temp in the Lab is around 18oC
} After cooling the chamber to 5oC and opening the Inlet valve, the
} pressure rises to 750psi at which is will remain for at least an hour with
} all valves closed.
} With the Inlet valve open, however, and waiting for liquid CO2 to
} show in the sight glass, the temperature begins to rise and does so to
} around 10oC at which point the fluid begins to rise in the glass to about
} 1/8 of the 1cm diameter.
} If I re-cool the chamber to 5oC, the fluid disappears.
} If I wait for up to 3 minutes, one of two things happens.
} 1. the fluid reappears and rises to 1/2 the sight window,
} or
} 2. I see nothing until I release the pressure and for a
} brief period note the fluid level show at the very top of the sight glass
} and fall and disappear very rapidly.
} After the first run, which I cannot control, I cannot merely recyle
} the unit. I have to wait an hour to demonstrate another unsatisfactory
} test.
}
} If anyone can help me ferret out this poltergeist, I would be most
} appreciative. I must say that I am convinced that I am dealing with a
} daemon from the PChem lab.



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 20 May 2002 12:49:34 -0500
Subject: Re: Chillers on the roof

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If it is simply a matter of recirculation, it shouldn't be a problem even
it there was a difference of several stories. Whether the pump is on the
roof or in the EM room, since the inlet and outlet are at approximately the
same height, there should be no net elevation head to be concerned about.

You would have the extra elevation head generating increased pressure in
the line at its low point, but that it would only amount to about 15 psi or
one bar for that height difference.

There may be some issue due to the length of the loop, but I will leave
that to someone with more experience in that area.

Warren

At 12:11 PM 5/19/02 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon May 20 13:47:14 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Mon, 20 May 2002 14:40:06 -0400
Subject: Scope Heads & Cameras

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who tried to help me with my head problem, I mean my microscope head problem ;-)

The guys from the company whose camera we bought came down and fixed the problem we were having with the camera. Now it looks like we're needing to buy a new LM, one that has the proper Trinoc head to handle our camera.


Again thanks to all who assisted me.


Looking at tiny things on big microscopes,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Mon May 20 14:37:23 2002



From: Mark Aindow :      m.aindow-at-uconn.edu
Date: Mon, 20 May 2002 15:29:34 -0400
Subject: Postdoctoral Positions

Contents Retrieved from Microscopy Listserver Archives
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University of Connecticut
Institute of Materials Science

Postdoctoral Positions

Two post-doctoral position are available immediately within the
Institute of Materials Science at the University of Connecticut. Both
positions require extensive hands-on experience in transmission electron
microscopy, scanning electron microscopy, and x-ray diffraction. These
positions are one-year appointments, with possible renewal for a second
year.

The first position is for work on characterization and synthesis of
ceramic coatings, thin films, and nanocomposites. Experience in the
areas of materials chemistry and synthesis/processing is highly
desirable. Contact: Prof. Nitin Padture at: nitin.padture-at-uconn.edu

The second position is for a study of the character and motion of
interfacial defects in titanium aluminide alloys. An individual with
prior experience in the use of HREM and analysis of interfacial
crystallography would be preferred. Contact Prof. Mark Aindow at
m.aindow-at-uconn.edu

To apply, please send a complete resume, together with a list of
publications and contact details for 3 referees to the appropriate
contact named above. Screening of applications will begin on June 1, and
will continue until the positions are filled. We encourage applications
from under-represented groups, including minorities, women and people
with disabilities.


--
*********************************************************

Mark Aindow, Associate Professor,
Department of Metallurgy and Materials Engineering
Institute of Materials Science,
97 North Eagleville Road, Unit 3136
University of Connecticut, Storrs,
CT 06269-3136, USA

Tel: +1 (860) 486-2644
FAX: +1 (860) 486-4745
Email: m.aindow-at-uconn.edu

**********************************************************




From daemon Mon May 20 15:09:47 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Mon, 20 May 2002 16:02:47 -0400
Subject: RE:Zeiss 902

Contents Retrieved from Microscopy Listserver Archives
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We are closing a Neurobiology EM lab and have an extra working Zeiss 902
available. If you are interested look at the official MSA website and the
Surplus Listing.

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954



From daemon Tue May 21 07:27:01 2002



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Tue, 21 May 2002 08:56:05 -0300
Subject: Chillers on the roof

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,
We have a Hitachi H7500, and a 3000-N. We use an air cooled
Haskaris chiller for both, and located the chiller in a well ventilated
rooftop service enclosure, approx. 12 metres above the EM room. In 2 years
we have had no problems with this set-up. Saves water, eliminates noise and
heat near the microscopes, plus the space savings.

David O'Neil
Institute for Marine Biosciences
National Research Council of Canada
1411 Oxford St.
Halifax, NS B3H 3Z1
ph. 902-426-8258
fax 902-426-9413
david.o'neil-at-nrc.ca
http://www.imb.nrc.ca/micros_e.html





-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: May 19, 2002 8:11 AM
To: microscopy-at-sparc5.microscopy.com


In about 6 months time I will have to move my microscopes into a newly
re-furbished two-storey building. The chillers for the FEI CM120 and
Hitachi 4700 may have to be located on the roof, at about 8.5 metres
from ground level. I would like to hear from anyone who has
considered this, and would particularly value the first-hand
experiences of anyone who has already tried it. Are the pumps in
standard chillers capable of dealing with an 8-metre head?

Chris



From daemon Tue May 21 12:54:22 2002



From: Jackie :      ujtxh-at-eszett.de
Date: Wed, 22 May 2002 01:15:32 +0900
Subject: Great shape for summer,get there now

Contents Retrieved from Microscopy Listserver Archives
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From daemon Tue May 21 12:54:22 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 21 May 2002 12:45:05 -0500
Subject: Replicas of glass surface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a customer who needs to determine whether
his glass and crystal objects were polished by hand
or with machines. The objects are too big to fit
in microscope, so I am looking for advice how to
prepare replicas of their surfaces which will not
leave marks on the objects.

Thank you,

Vladimir Dusevich


From daemon Tue May 21 13:06:07 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 21 May 2002 13:59:49 -0400
Subject: RE: Ask-A-Microscopist: corn pollen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Karen,
Thought I remembered something from history about pollen processing,
but......specifics are lost. I think I found a relevant reference.
----------------------------------------------------------------- Immunogold electron microscopy of soluble proteins: localization of Bet v I
major allergen in ultra-thin sections of birch pollen after anhydrous
fixation techniques M Grote Institute of Medical Physics, Munster University, Germany. To localize the highly water-soluble major allergen Bet v I in ultra- thin
sections of birch pollen, pollen grains were cracked, air-dried, and
processed for electron microscopy using one of the following preparation
techniques: fixation in aqueous p-formaldehyde + cetylpyridinium chloride;
fixation in p-formaldehyde vapor; fixation in benzoquinone vapor; inert
dehydration; or no fixation. Afterwards the pollen grains were embedded in
Lowicryl K4M resin at low temperature. Ultra-thin sections were cut and
incubated with a monoclonal antibody against Bet v I, followed by a
gold-labeled secondary antibody. In some experiments, commercial rabbit IgG
antibodies against birth pollen allergens were also used, followed by
incubation with the protein A- gold complex. Bet v I could be localized only
after vapor fixation and in the inert dehydrated specimens. Best
preservation of ultrastructure and antigenicity was obtained after
p-formaldehyde vapor fixation. Bet v I antibody binding sites were detected
only in the cytoplasmic matrix of the pollen grain, never in the pollen
wall. Commercial rabbit antibodies bound to cytoplasm and wall of all
prepared specimens, even after aqueous fixation. This might be explained by
the assumption that these antibodies recognize a variety of antigenic and
allergenic structures, not all of which are so highly soluble as Bet v I. Volume 39, Issue 10, pp. 1395-1401, 10/01/1991 Copyright © 1991 by The Histochemical Society

Hope this gives you another starting point.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: kchamusco-at-mail.ifas.ufl.edu
} Sent: Saturday, May 18, 2002 9:56 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: corn pollen
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (kchamusco-at-mail.ifas.ufl.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
} May 18, 2002 at 16:01:58
} --------------------------------------------------------------------------
} -
}
} Email: kchamusco-at-mail.ifas.ufl.edu
} Name: karen chamusco
}
} Organization: U of FL
}
} Education: Graduate College
}
} Location: Gainesville FL
}
} Question: I'm sorry if I should be usinf the list serve but this is
} the only page I could find to ask a question. I have been working
} with corn pollen and trying to embed it in LR White resin for
} immunolabeling in TEM. I thought I had this problem solved, but,
} apparently I don't. When I get my pollen through the EtOH series up
} to 95% it looks very good, but when I try to embed it in the resin it
} collapses. I have tried putting it in resin/EtOH at 20% steps and
} directly embedding it in 100% resin. The 20% stepwise series seems to
} be the least destructive and I can get partially collapsed pollen
} sometimes. I really need to be able to see inside a "fluffy" whole,
} uncollapsed pollen grain. Is there anyone who can help with this
} before I pull out the rest of my hair? If necessary to know, I fix
} in 4% paraformaldehyde + 0.5% gluteraldehyde + 4% sucrose + 0.3%
} tween {to get the samples to sink}. I vacuum infiltrate for 5 minutes
} then I leave them in the fridge overnight. I then rinse with PBS and
} send through a 10% EtOH series{anything greater causes the pollen to
} collapse}. Then the rest is as I described above. Thanks, Karen
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Tue May 21 17:14:22 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 21 May 2002 20:22:41 -0500
Subject: Replicating glass surfaces

Contents Retrieved from Microscopy Listserver Archives
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Vladimir -

A conservator or scientists at the Corning Museum of Glass can advise you.
Their main number is 800.732.6845.

James Martin
Conservation Scientist


----- Original Message -----
} From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
To: "microscopylistserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, May 21, 2002 1:45 PM


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Vladimir Dusevich wrote:
============================================================
I have a customer who needs to determine whether his glass and crystal
objects were polished by hand or with machines. The objects are too big to
fit in microscope, so I am looking for advice how to prepare replicas of
their surfaces which will not leave marks on the objects.
============================================================
We have done something very similar to this, the first time going back into
the mid-1970's. We used what was then the prototype of the SPI Supplies :
"Wet Replica Kit" as shown on URL
http://www.2spi.com/catalog/spec_prep/wet_rep_kits.shtml

I realize this is not a wet surface but the kit was originally developed for
the replication of a "wet" surface such as a plant leaf or even human skin.

Since it is a silicone, so long as the components are properly mixed, there
should be no residue left behind on the surface. And the lift-off is easy
enough that one does not generally need any kind of metal tools (e.g. a
scalpel blade or tweezers) to help get the left off started.

We have also tried the cellulose acetate replica approach, see URL
http://www.2spi.com/catalog/submat/cellulose-acetate-replicating-tape-sheets
.shtml
but we have had greater difficulty getting the cellulose acetate to lift off
without the use of some kind of sharp instrument to start the lift-off.

Another advantage of the Wet Replica kit is that the replica itself can be
replicated so that what goes into the SEM is something that spatially, looks
just like the actual surface.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Wed May 22 00:56:22 2002



From: Colin.Veitch-at-csiro.au
Date: Wed, 22 May 2002 15:45:58 +1000
Subject: OM: shading on a Lieca digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

This question is on behalf of our OM operator.

We have a Leitz DMRBE OM and have just purchased a Leica DC 300F digital
camera to use with it. The engineer recommended we get a x0.63 C-mount
adaptor to use with the camera, particularly for fluorescence work "to
concentrate the low light levels".

When using the camera with the x0.63 adaptor we get a dark shading on one
side of the image(approximately 25% of the width of the image). This
shading is independent of the camera alignment (ie you can rotate the camera
and the shading doesn't rotate (although the image does)) and it also
appears to be independent of the alignment of the adaptor. (ie you can
rotate the adaptor and the shading doesn't rotate)

If you use a plain tube adaptor or a 1x adaptor there is no evidence of this
shading. We have also tried the camera on other microscopes with identical
results (ie shading only when using the x0.63 adaptor). We have also tried
two different x0.63 adaptors and the shading was the same.

In the manual it mentions that some vignetting MAY occur using the x0.63
adaptor. Is this what we are seeing? The manual doesn't offer a solution
other than not using the x0.63 adaptor. Does anyone have any ideas how we
may be able to get around this problem other than not using the adaptor?

Thank you very much in advance.



Colin Veitch

Instrumentation Scientist
Late Stage Innovation Group
CSIRO Textile and Fibre Technology
PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au
Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Fax: +61 (0) 3 5246 4811


The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may not
copy, distribute or take any action in reliance on it. If you have received
this message in error, please telephone CSIRO Textile and Fibre Technology
on +61 3 5246 4000.



From daemon Wed May 22 02:53:07 2002



From: Visitec-at-t-online.de (Martin Klein)
Date: Wed, 22 May 2002 09:39:39 +0200
Subject: AW: Replicas of glass surface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Vladimir,

I would like to offer you the opportunity to put your samples into our large
chamber SEM. There is enough space for glass and crystal objects up to a
volume of 1 cubic meter and 500 pounds of weight. Further more we can
analyze the chemical composition of the glass surfaces by EDS.

Especially the analytic capabilities might be an advantage in distinguishing
the production method.

Best regards
Martin Klein


VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen/Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: mklein-at-visitec-em.de
WWW: http://www.visitec-em.de
++++ Home of the world's largest SEM ++++

-----Ursprüngliche Nachricht-----
Von: Dusevich, Vladimir [mailto:DusevichV-at-umkc.edu]
Gesendet: Dienstag, 21. Mai 2002 19:45
An: microscopylistserver
Betreff: Replicas of glass surface


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have a customer who needs to determine whether
his glass and crystal objects were polished by hand
or with machines. The objects are too big to fit
in microscope, so I am looking for advice how to
prepare replicas of their surfaces which will not
leave marks on the objects.

Thank you,

Vladimir Dusevich



From daemon Wed May 22 06:19:21 2002



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Wed, 22 May 2002 11:59:57 +0100
Subject: Scandiplast

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know of the cold mounting resisn called 'Scandiplast' and
who supplies it?

Thanks in advance,
Steven

--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Wed May 22 08:34:30 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 22 May 2002 08:23:38 -0500
Subject: Re: Replicas of glass surface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a user with the same basic need, only looking at marks on
ancient ceramics. These are museum pieces and cannot be altered. He
makes casts with latex or wax, usually latex.

Phil

} I have a customer who needs to determine whether
} his glass and crystal objects were polished by hand
} or with machines. The objects are too big to fit
} in microscope, so I am looking for advice how to
} prepare replicas of their surfaces which will not
} leave marks on the objects.
}
} Thank you,
}
} Vladimir Dusevich

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Wed May 22 09:15:12 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 22 May 2002 08:14:42 -0600
Subject: OM: shading on a Lieca digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colin,

You use a .63x tube normally to adapt the field of view of the camera to the
field of view through the binoculars. I am not sure what that has to do with
"concentrating the light". And the physical reason behind that is, that the
camera uses a chip that is bigger than the standard TV chips. Some
vignetting (darker edges) is indeed normal when you use such a setup, but
you may be able to minimize it by carfully adjusting all the parameters of
the microscope and illumination path. In particular, it should be symmetric.
Since you are using a digital camera, you can correct the images by using a
shading or background correction. That's a software feature, and you may
have to check if it is supported. If not, you can take a background image by
removing the sample and taking a picture. then take a normal picture with
your sample and divide or subtract the background image from the regular
image.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "Colin.Veitch-at-csiro.au"-at-sparc5.microscopy.com
[mailto:"Colin.Veitch-at-csiro.au"-at-sparc5.microscopy.com]
Sent: Tuesday, May 21, 2002 11:46 PM
To: microscopy-at-sparc5.microscopy.com


Hi All,

This question is on behalf of our OM operator.

We have a Leitz DMRBE OM and have just purchased a Leica DC 300F digital
camera to use with it. The engineer recommended we get a x0.63 C-mount
adaptor to use with the camera, particularly for fluorescence work "to
concentrate the low light levels".

When using the camera with the x0.63 adaptor we get a dark shading on one
side of the image(approximately 25% of the width of the image). This
shading is independent of the camera alignment (ie you can rotate the camera
and the shading doesn't rotate (although the image does)) and it also
appears to be independent of the alignment of the adaptor. (ie you can
rotate the adaptor and the shading doesn't rotate)

If you use a plain tube adaptor or a 1x adaptor there is no evidence of this
shading. We have also tried the camera on other microscopes with identical
results (ie shading only when using the x0.63 adaptor). We have also tried
two different x0.63 adaptors and the shading was the same.

In the manual it mentions that some vignetting MAY occur using the x0.63
adaptor. Is this what we are seeing? The manual doesn't offer a solution
other than not using the x0.63 adaptor. Does anyone have any ideas how we
may be able to get around this problem other than not using the adaptor?

Thank you very much in advance.



Colin Veitch

Instrumentation Scientist
Late Stage Innovation Group
CSIRO Textile and Fibre Technology
PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au
Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Fax: +61 (0) 3 5246 4811


The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may not
copy, distribute or take any action in reliance on it. If you have received
this message in error, please telephone CSIRO Textile and Fibre Technology
on +61 3 5246 4000.



From daemon Wed May 22 09:19:15 2002



From: barbara maloney :      bmalon01-at-fiu.edu
Date: Wed, 22 May 2002 10:10:21 -0400
Subject: SEM technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everybody - what is the best method for prep on a dried out moth?
Just Au coat or if you have low vacuum mode available - go with that
uncoated?
Thanks
Barb



From daemon Wed May 22 10:39:42 2002



From: Joyce Craig :      J-Craig-at-csu.edu
Date: Wed, 22 May 2002 10:23:13 -0700
Subject: Chillers on the roof

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How difficult will it be to get up on the roof to check the chillers for
water level? I check mine weekly.
Will the water lines freeze in the winter?
Joyce Craig
Chicago State University



From daemon Wed May 22 10:45:02 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 22 May 2002 10:32:34 -0500
Subject: Toluidine Blue question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Regarding a Toluidine Blue stain solution:

1% Toluidine Blue O
1% Sodium Tetraborate Dodecahydrate
30% Ethanol
in DDI

I've been using this stain for years on Epon-Araldite sections and Durcupan
sections with no problems in getting satisfactory staining. When I have
30-50mL remaining, I always make a new batch so it's ready the next time I
need it , but this time I forgot. I made a new solution and had to use it
right away. I stained my sections as usual, but the color of the sections
was bright blue instead of the more purple-blue we desire. Thinking that
I'd made a mistake in preparing the solution, I made another batch and
decided to wait overnight to try it, but again the sections were bright
blue.

I was never aware of the need to do this, but does this solution need to
"ripen" for a week or so before using? I can't think of any other reason.
The quality of our water hasn't changed, and everything else is the same as
I've always used.

Thank you for any help you can give me.

Jaclynn M. Lett, Research Technician
Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu
314-977-0257


From daemon Wed May 22 10:51:47 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Wed, 22 May 2002 08:45:55 -0700
Subject: Re: Replicas of glass surface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You could try dental impression materials, available from any dental
supplier. There are various types, I think the best type for your purpose
would be one of the silicone-based ones. This would give a negative
impression, of course. To get a positive I use epoxy resins: place the
silicone impression at the bottom of a small container and pour a de-bubbled
resin to make a layer 2 or 3 mm thick. After curing, the resin impression is
indistinguishable from the original in the SEM. Hope this helps.

Lesley Weston.


on 21/05/2002 10:45 AM, Dusevich, Vladimir at DusevichV-at-umkc.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a customer who needs to determine whether
} his glass and crystal objects were polished by hand
} or with machines. The objects are too big to fit
} in microscope, so I am looking for advice how to
} prepare replicas of their surfaces which will not
} leave marks on the objects.
}
} Thank you,
}
} Vladimir Dusevich
}



From daemon Wed May 22 12:34:20 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Wed, 22 May 2002 18:28:37 +0100
Subject: Fw: SEM technique for moths

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Butterlies and moths, and other scaly insects such as some beetles
etc. can be a right pain.
The overlapping scales make it almost impossible to put down a
continuous coating.
Even well-coated specimens may give problems with charging. If the
whole moth is the objective,
then the underside needs to be coated as well as the top. I
contributed a tip for doing that with hard objects like seeds etc.
some time ago,
but the method I described then will damage a moth. The simplest
thing
to do with a moth is to coat the underside before mounting. Once
mounted coat the top. The more angles you coat from the better, so
rotary coating will help, even in a sputter coater. Carbon seems to
reach the parts other coating materials cannot reach, so a
preliminary
rotary/tilting coating with carbon before sputtering may improve the
overall conductivity. I used to do that routinely, but it has gone
out
of favour, mostly for good reasons.
Low vac mode may help somewhat, but not if you need high res images
of
scale details. Keep the kV and beam current as low as possible, use
short exposures if you have enough signal to do so. In the worst-case
scenario collecting an image by frame-integration at tv rate may be
the only way, but image quality is rarely very good. Try a little
specimen bias if available. Use a FEG SEM if available. Use the lower
detector if there is a choice.

Chris

} ----- Original Message -----
} From: "barbara maloney" {bmalon01-at-fiu.edu}
} To: "'microscopy-at-msa.microscopy.com'"
} {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, May 22, 2002 3:10 PM
} Subject: SEM technique
}
}
}
} --------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
} ---.
} }
} }
} } Hi everybody - what is the best method for prep on a dried out
moth?
} } Just Au coat or if you have low vacuum mode available - go with
that
} } uncoated?
} } Thanks
} } Barb
} }
} }
}



From daemon Wed May 22 13:23:39 2002



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 22 May 2002 13:16:34 -0500
Subject: RE: SEM technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If specimen permits, I always coat it. Images are much
better for coated specimens.

Vladimir

} -----Original Message-----
} From: barbara maloney [mailto:bmalon01-at-fiu.edu]
} Sent: Wednesday, May 22, 2002 9:10 AM
} To: 'microscopy-at-msa.microscopy.com'
} Subject: SEM technique
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi everybody - what is the best method for prep on a dried out moth?
} Just Au coat or if you have low vacuum mode available - go with that
} uncoated?
} Thanks
} Barb
}
}
}


From daemon Wed May 22 14:46:29 2002



From: Anne-Marie Brun :      abrun-at-hsc.unt.edu
Date: Wed, 22 May 2002 14:38:23 -0500
Subject: Microscopic user fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would any one please share with use your user fees of your microscopic
facility including charges for imaging? I would appreciate your help. We
are in the process of establishing such charges and hope to find
willingness of your part to share this information.

Many thanks.

Anne-Marie Brun
Research Associate
Mol. Biology and Genetics
UNT HSC
Fort Worth, Texas



From daemon Wed May 22 15:33:48 2002



From: Eric Johnston :      ericdj-at-seas.upenn.edu
Date: Wed, 22 May 2002 16:21:38 -0400
Subject: Hot spot in epifluorescence - update

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Hi all

I have an update to my hotspot problem that unfortunately does not include
any solutions.

First of all, we don't get the problem on the transmitted side of the
scope. The cheap halogen lamp seems to work fine. Also, we have another
Nikon TE300 microscope in the lab with the same Coolsnap HQ camera and we
get exactly the same thing. That seems rather suspicious.

I think I can see this effect with a regular video camera if I threshold
the image.

Here is a list of things we have done so far. None of them have produced
any appreciable change.

1) 10 degree holographic diffuser in place of one of the neutral density
filters right after the lamp.
2) IR filter in the collector lens.
3) IR filter before the camera.
4) aligned and misaligned the lamp in every possible way.
6) focused and defocused the lamp in every possible way.
7) changed out filter block.
8) reduced the exposure time of the camera down to 1 ms.

So it doesn't seem to be IR.

I'll let you know if we figure it out.

Eric




Eric Johnston
Design Engineer and Research Specialist
Department of Bioengineering
University of Pennsylvania
5170 Vagelos
220 South 33rd Street
Philadelphia, PA 19104
215-573-6696
215-573-7616
(F) 215-573-2071
ericdj-at-seas.upenn.edu



From daemon Wed May 22 15:44:15 2002



From: jtwilley-at-sprynet.com
Date: Wed, 22 May 2002 16:37:54 -0400
Subject: Re: AW: Replicas of glass surface

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Mr. Klein,

You should be aware, as should anyone else who might attempt this, that certain glass and rock crystal artifacts are prone to catastrophic fracturing when subjected to pressure or humidity changes - crystal in the first case and glass in the second.

Rock crystal artifacts are often subject to considerable residual stress and must be protected from sudden temperature or pressure excursions. Certain glasses, many from archaeological origins but also some from the 19th Century, are unstable with respect to water and may have undergone partial hydrolysis with a resulting incorporation of hydrous silica in places formerly occupied by alkali-oxygen-silica links. Such glasses are sometimes visually unchanged but may shatter to powder when subjected to dehydrating conditions as under the vacuum of a SEM chamber.

While the advantages of your large chamber instrument are well known, the examination of replicas is safer unless a great deal is known about the behavior of the specific object.

John Twilley
Art Conservation Scientist

On Wed, 22 May 2002 09:39:39 +0200 Martin Klein {Visitec-at-t-online.de} wrote:

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Dear Vladimir,

I would like to offer you the opportunity to put your samples into our large
chamber SEM. There is enough space for glass and crystal objects up to a
volume of 1 cubic meter and 500 pounds of weight. Further more we can
analyze the chemical composition of the glass surfaces by EDS.

Especially the analytic capabilities might be an advantage in distinguishing
the production method.

Best regards
Martin Klein


VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen/Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: mklein-at-visitec-em.de
WWW: http://www.visitec-em.de
++++ Home of the world's largest SEM ++++

-----Ursprüngliche Nachricht-----
Von: Dusevich, Vladimir [mailto:DusevichV-at-umkc.edu]
Gesendet: Dienstag, 21. Mai 2002 19:45
An: microscopylistserver
Betreff: Replicas of glass surface


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I have a customer who needs to determine whether
his glass and crystal objects were polished by hand
or with machines. The objects are too big to fit
in microscope, so I am looking for advice how to
prepare replicas of their surfaces which will not
leave marks on the objects.

Thank you,

Vladimir Dusevich




From daemon Wed May 22 16:12:25 2002



From: cwuethri-at-caregroup.harvard.edu
Date: Wed, 22 May 2002 17:05:06 -0400
Subject: Toluidine blue

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Dear Jaclynn,

Try perhaps to control the pH of your Toluidine Blue stain solution.

If I remember well, Toluidine Blue Stain is metachromatic when it is acidic
(no real difference between pH 4 and 7) but it is no more (only blue) when
basic (for example at pH 8.2, at least for plant tissue embeded in JB4).

Hope it helps !

Chris Wuethrich
Beth Israel Deaconess Medical Center
330 brookline AVE
Boston, MA 02215
cwuethri-at-caregroup.harvard.edu


From daemon Wed May 22 18:39:21 2002



From: RCHIOVETTI-at-aol.com
Date: Wed, 22 May 2002 19:31:08 EDT
Subject: Re: OM: shading on a Lieca digital camera

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In a message dated 05/21/2002 11:02:03 PM US Mountain Standard Time,
Colin.Veitch-at-csiro.au-at-sparc5.microscopy.com writes:

{ { When using the camera with the x0.63 adaptor we get a dark shading on one
side of the image(approximately 25% of the width of the image). This
shading is independent of the camera alignment (ie you can rotate the camera
and the shading doesn't rotate (although the image does)) and it also
appears to be independent of the alignment of the adaptor. (ie you can
rotate the adaptor and the shading doesn't rotate) } }

Colin,

I know the Leica DC500 has a "shading correction" routine you can use to even
out the illumination, but I don't know if the 300F has the same routine. We
had a similar problem with a 0.63X adapter on a DC500, and running the
"shading correction" took care of the problem.

If the camera can do this (check the manual), you will see a "shading"
function on the left side of the screen where you set the image size, auto
contrast, etc. Proceed as follows:

Click on "shading" and first set the black level: shut off all light to the
camera and click on "black level." After this, click on "shading" again and
then set the white level by sending light to the camera and making sure there
is no specimen or tissue in the field of view. Then, grab a "white level."
You may have to play with the "brightness" slider at the top of the window
until the camera accepts the bright level. It will inform you if the
illumination is too dark or too light.

Also, when you set the white level you will have to assign a name to it...you
can call it "10x," or "40x," or whatever, for the objective you are using, or
maybe "brightfield" or another name of your choice.

If you have access to this routine on the 300F, it should take care of
moderate differences in illumination such as hot spots and shadows.

If the shading is severe, it may be too much for the correction factor to
handle. If this is the case, you will probably either need to use a 1.0X
adapter or a Leica c-mount "vario-zoom" adapter, which allows you to set the
magnification from about 0.3 up to 1.3 or so, much like a zoom lens for a
35mm camera.

Good luck, let us know how things work out!

Cheers,

Bob Chiovetti


From daemon Wed May 22 21:23:28 2002



From: barbara maloney :      bmalon01-at-fiu.edu
Date: Wed, 22 May 2002 22:14:35 -0400
Subject: Moth prep

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Hi everybody - thanks to those who responded. Wanted to let you know,
we ended (before I received your responses) coating 367 Ang of Au for 2
min. Prior to coating we did view at low vacuum with no coat - got an
image, of course not as nice as when we coated at high vacuum. Just
used a large carbon tab on viewed ventral side of moth - really nice
images. Thanks gang.
Barb
P.S. I will try your suggestions. Thanks again



From daemon Wed May 22 22:42:55 2002



From: Sue Lindsay :      suelind-at-austmus.gov.au
Date: Thu, 23 May 2002 13:46:46 +1000
Subject: Re: SEM technique

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barbara maloney wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi everybody - what is the best method for prep on a dried out moth?
} Just Au coat or if you have low vacuum mode available - go with that
} uncoated?
} Thanks
} Barb

Hi Barb
I routinely deal with insects especially hairy and scaly specimens. What I
have found is,
firstly when you mount the specimen - I always mount the whole animal on a
pin - use carbon or silver impregnated glue to stick the moth onto the pin
or directly onto a stub.
Secondly if mounting directly onto an aluminum stub, I use a double sided
carbon sticky tab on the stub then apply the glue to the tab then the
specimen. This ensures good conductivity.
Thirdly when coating be careful not the coat too heavily with gold - you
will loose some fine detail on the scales, antennae etc. Coat the specimen
several times (short coating times) in differing positions - this will
ensure total coverage. With the specimen on a pin it makes coating the whole
specimen a lot easier.
Once coated I then recoat the pin with the carbon glue. This makes the pin
nonconductive and once in the SEM you do not see the pin. Thus the moth
looks like it is sitting in space.

We use a LEO 435VP SEM in high vacuum mode to do this work. The way I avoid
charging (if it is charging much at all) is to use the Robinson Backscatter
Detector. The images derived are fantastic. Quite contrasty!!!
Another thing is I often mix the Backscatter signal with the Secondary
signal. This allows me to enhance the background, bring out the edges, seta
etc that appear to be flattened in the backscatter image.

I hope this helps

Sue

--
Sue Lindsay

SEM Laboratory Manager
Scanning Electron Microscope Unit
The Australian Museum
6 College st
Sydney, 2010
NSW, Australia

ph 02 9320 6198
fax 02 9320 6059
Email suelind-at-austmus.gov.au




From daemon Wed May 22 22:42:56 2002



From: Sue Lindsay :      suelind-at-austmus.gov.au
Date: Thu, 23 May 2002 13:46:46 +1000
Subject: Re: SEM technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




barbara maloney wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi everybody - what is the best method for prep on a dried out moth?
} Just Au coat or if you have low vacuum mode available - go with that
} uncoated?
} Thanks
} Barb

Hi Barb
I routinely deal with insects especially hairy and scaly specimens. What I
have found is,
firstly when you mount the specimen - I always mount the whole animal on a
pin - use carbon or silver impregnated glue to stick the moth onto the pin
or directly onto a stub.
Secondly if mounting directly onto an aluminum stub, I use a double sided
carbon sticky tab on the stub then apply the glue to the tab then the
specimen. This ensures good conductivity.
Thirdly when coating be careful not the coat too heavily with gold - you
will loose some fine detail on the scales, antennae etc. Coat the specimen
several times (short coating times) in differing positions - this will
ensure total coverage. With the specimen on a pin it makes coating the whole
specimen a lot easier.
Once coated I then recoat the pin with the carbon glue. This makes the pin
nonconductive and once in the SEM you do not see the pin. Thus the moth
looks like it is sitting in space.

We use a LEO 435VP SEM in high vacuum mode to do this work. The way I avoid
charging (if it is charging much at all) is to use the Robinson Backscatter
Detector. The images derived are fantastic. Quite contrasty!!!
Another thing is I often mix the Backscatter signal with the Secondary
signal. This allows me to enhance the background, bring out the edges, seta
etc that appear to be flattened in the backscatter image.

I hope this helps

Sue

--
Sue Lindsay

SEM Laboratory Manager
Scanning Electron Microscope Unit
The Australian Museum
6 College st
Sydney, 2010
NSW, Australia

ph 02 9320 6198
fax 02 9320 6059
Email suelind-at-austmus.gov.au




From daemon Thu May 23 03:16:15 2002



From: newsletter-at-metalfirst.com :      newsletter-at-metalfirst.com
Date: Tue, 21 May 2002 20:47:41 +0800
Subject: MetalFirst.Com

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From daemon Thu May 23 07:57:38 2002



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Thu, 23 May 2002 08:55:58 -0400
Subject: TEM-sectioning insect material

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A student working in the lab is interested in using TEM to examine
thickness of the chitinous exoskeleton and the interface between muscle and
skeletal attachments in some insects (Heteroptera). Does someone have
experience in embedding and ultramicrotomy of this type of material? I
expect we will have some problems with the harder parts pulling out of the
resin. Are there any tricks we should know about? Any special fixation
steps or suggestions? Thanks.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2242
University of Connecticut
Storrs, CT 06269-2242
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Thu May 23 08:22:14 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 23 May 2002 09:15:12 -0400
Subject: RE: Microscopic user fees

Contents Retrieved from Microscopy Listserver Archives
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} Morning Anne-Marie,
} When I need such information, I try searching Google for {core
} facility charges} . Pages and pages.
}
} Regards,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} West Chester University
} South Church Street and Rosedale
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
}
} ----------
} From: Anne-Marie Brun
} Sent: Wednesday, May 22, 2002 3:38 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Microscopic user fees
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Would any one please share with use your user fees of your microscopic
} facility including charges for imaging? I would appreciate your help. We
} are in the process of establishing such charges and hope to find
} willingness of your part to share this information.
}
} Many thanks.
}
} Anne-Marie Brun
} Research Associate
} Mol. Biology and Genetics
} UNT HSC
} Fort Worth, Texas
}
}
}


From daemon Thu May 23 08:30:53 2002



From: Emmanuelle Roux :      eroux-at-caprion.com
Date: Thu, 23 May 2002 09:22:51 -0400
Subject: Toluidine blue

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Hi,
Here is the way we do the TB:
1% TB in H2O
Saturate with Na Borate (Na2B4O7.10H2O) (25g for 500ml)
Leave 24h at 4 C
Filter with Whatman No.1, 2 times
Leave at 4 C for a week before use.
Filter with a syringe filter before each use.
Keep at 4 C. Protect from light.
Hope it helps.
Emmanuelle.

Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620



From daemon Thu May 23 08:33:52 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 23 May 2002 09:22:33 -0400
Subject: Re: Microscopic user fees

Contents Retrieved from Microscopy Listserver Archives
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Hi Ann-Marie,
You can go to our website for the fees for all of the core facilities
here at Weill-Cornell:
www.med.cornell.edu/research/cores

specifically the EM core can be founds at the above address plus:
/core1/sop.html
and our oprical core facility is at: /core3/sop.html
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu May 23 08:44:34 2002



From: jshields-at-cb.uga.edu
Date: Thu, 23 May 2002 09:36:41 -0400
Subject: x-ray photoctron?

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Hi All,
Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
please provide brief description and some citations.
Thanks!

John Shields
EM Lab
Univ. of Georgia
Athens, GA 30602


From daemon Thu May 23 08:54:50 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 23 May 2002 09:48:29 -0400
Subject: RE: Toluidine Blue question

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Morning Jaclynn,

Well, let's see. What I know is that to preserve metachromasia -
Toluidine blue is one of the thazine dyes that is known for metachromatic
staining - one is admonished NOT to use ethanol - I use tertiary
butanol(TBA) - to dehydrate/differentiate a section. Of course, in
semi-thin staining, most procedures do not suggest anything but rinsing
with water followed by drying before mounting - if that is how you prepare
the semi-thin for viewing.

I have never put ethanol in my Toluidine blue stains. With the
above in mind as well as the normal semi-thin staining protocols with which
I am familiar, if I did add ethanol it would be to restrict/prevent
metachromasia in the preparation.

Why aging? I can't give an all-knowing response, but if the ethanol
slows some coordination of the buffer with the dye, then that would be an
explanation.

I presume your recipe is something like this:

1g TolBlue
1g Sodium borate
30ml 100% ethanol
70ml water(d.i.)

If so then we are on the same page. Perhaps what you need to do is
add the ethanol, if you continue to use it, sometime (hours?) after you make
up the aqueous dye-buffer solution.

My recipe comes from a small Lab Manual by: Giuseppe
Millonig(1976), Laboratory Manual of Biologic electron Microscopy, Mario
Saviolo - Editore, Vercelli C.P. 182, Italy.


Method 3(Millonig Ref: Richardson, K.C., Jarret, L, Finke,
E.H.(1960), Embedding in epoxy resins for ultrathin sectioning in electron
microscopy, Stain. tech., 35: 313)

0.5g sodium borate
50ml water

0.5g Toluidine Blue [O] - C.I. 52040) - You should check -
Lillie, R.D.(Ed.)(1977), H.J. Conn's Biological Stains(9th Ed.), Williams
and Wilkins, Baltimore - for information on contrast between old and new
dyes with this name.

Above sequence as given in Millonig.

I often use it fresh, and I can't ever remember a problem,
even though, like you, I tend to make it in advance of need, at least by a
day.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Jaclynn Lett
} Sent: Wednesday, May 22, 2002 11:32 AM
} To: Microscopy Listserver (E-mail)
} Subject: Toluidine Blue question
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Regarding a Toluidine Blue stain solution:
}
} 1% Toluidine Blue O
} 1% Sodium Tetraborate Dodecahydrate
} 30% Ethanol
} in DDI
}
} I've been using this stain for years on Epon-Araldite sections and
} Durcupan
} sections with no problems in getting satisfactory staining. When I have
} 30-50mL remaining, I always make a new batch so it's ready the next time I
} need it , but this time I forgot. I made a new solution and had to use it
} right away. I stained my sections as usual, but the color of the sections
} was bright blue instead of the more purple-blue we desire. Thinking that
} I'd made a mistake in preparing the solution, I made another batch and
} decided to wait overnight to try it, but again the sections were bright
} blue.
}
} I was never aware of the need to do this, but does this solution need to
} "ripen" for a week or so before using? I can't think of any other reason.
} The quality of our water hasn't changed, and everything else is the same
} as
} I've always used.
}
} Thank you for any help you can give me.
}
} Jaclynn M. Lett, Research Technician
} Electron Microscopy Core Facility
} Fay and Carl Simons Center for Biology of Hearing and Deafness
} Central Institute for the Deaf
} 4560 Clayton Ave.
} St. Louis, MO 63110
}
} jlett-at-cid.wustl.edu
} 314-977-0257
}
}


From daemon Thu May 23 09:01:52 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 23 May 2002 10:47:32 -0500
Subject: diamond knife on cpd. microscope

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I am asking for your input once again. I am looking for a
source to obtain
"a diamond knife that can be installed onto a compound microscope
like a lens so that it can be used to cut a tiny circle on a plastic
membrane on a glass slide. One would look for a particular cell or
tissue with the desired properties using a regular lens (40X or
100X), and then switch to the knife, which is just a little off
center, and turn the knife around the central axis to score the
around the sample. A little buffer would then float the cut sample,
which can then be picked up by a TEM grid for observation using TEM."
Rosemary

--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Thu May 23 09:11:56 2002



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Thu, 23 May 2002 10:04:49 -0400
Subject: RE: SEM Technique

Contents Retrieved from Microscopy Listserver Archives
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Dear Barbara,

I would coat the dried moth with gold or gold/palladium and it should be
fine.

Good Luck,

Jackie Garfield
LifeCell Corp.


From daemon Thu May 23 09:31:16 2002



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Thu, 23 May 2002 10:24:05 -0400
Subject: Re: x-ray photoctron?

Contents Retrieved from Microscopy Listserver Archives
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It sounds like X-ray photoelectron spectroscopy (aka ESCA).

You use a monochromatic x-ray beam (usually Al or Mg target) to irradiate a
surface. The x-rays create photoelectrons which have characteristic
energies. Because the photoelectrons are low energy, the only ones to
escape come from the top 1-5nm of the sample. Hence the technique is a
surface measurement technique. By careful measurement of the electron
energies, the bonding state of the element can be determined, helping to
identify the chemical compound.

You might check out the book by Czenderna (or Czanderna) and articles by
M.P. Seah.

At 09:36 AM 5/23/02 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Thu May 23 10:17:13 2002



From: Keith R. Hallam :      k.r.hallam-at-bristol.ac.uk
Date: Thu, 23 May 2002 16:06:23 +0100
Subject: Re: x-ray photoctron?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sender: "Hendrik O. Colijn" {colijn.1-at-osu.edu}

} It sounds like X-ray photoelectron spectroscopy (aka ESCA).

} You might check out the book by Czenderna (or Czanderna) and articles
} by
} M.P. Seah.

And UK Surface Analysis Forum website at www.uksaf.org

Keith

----
Dr. Keith R. Hallam University of Bristol, Interface Analysis Centre,
Oldbury House, 121, St. Michael's Hill, Bristol, BS2 8BS, England
Telephone: + 44 (0)117 925 5666 | E-mail: k.r.hallam-at-bristol.ac.uk
Facsimile: + 44 (0)117 925 5646 | URL: http://eis.bris.ac.uk/~phkrh/





From daemon Thu May 23 11:05:21 2002



From: barbara maloney :      bmalon01-at-fiu.edu
Date: Thu, 23 May 2002 11:54:48 -0400
Subject: laser ablation

Contents Retrieved from Microscopy Listserver Archives
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Hi everybody - help!! I have a 2mm thick silicate glass sample - with
laser ablations in the diameter of ~50 um. We are expecting depth
measurement of ~70um. We want to view side of conical crater formed by
the laser, however after coating with 367 ang of Au, using SEI and BEI
not really seeing what we expected. On light scope, able to view the
conical crater. Any suggestions to what we can do to get a SEI image?
Thanks so much
Barb



From daemon Thu May 23 11:10:07 2002



From: eld26-at-cornell.edu
Date: Thu, 23 May 2002 12:03:33 -0400 (EDT)
Subject: Re: x-ray photoctron? (XPS)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Vickerman's surface analysis book gives a pretty good overview of XPS,
AES, etc. The title is something like Surface Analysis: the Principal
Techniques.

Eve


On Thu, 23 May 2002 jshields-at-cb.uga.edu-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
} Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
} please provide brief description and some citations.
} Thanks!
}
} John Shields
} EM Lab
} Univ. of Georgia
} Athens, GA 30602
}
}



From daemon Thu May 23 11:13:13 2002



From: eld26-at-cornell.edu
Date: Thu, 23 May 2002 12:06:41 -0400 (EDT)
Subject: Re: x-ray photoctron?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

Vickerman's book on surface analysis gives a pretty good overview of XPS,
AES, etc. The title is something like Surface Analysis: the Principal
Techniques.

Eve

On Thu, 23 May 2002 jshields-at-cb.uga.edu-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
} Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
} please provide brief description and some citations.
} Thanks!
}
} John Shields
} EM Lab
} Univ. of Georgia
} Athens, GA 30602
}
}



From daemon Thu May 23 12:37:30 2002



From: James.Passmore-at-sealedair.com
Date: Thu, 23 May 2002 13:29:16 -0400
Subject: Re: x-ray photoctron?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Looks like a typo to me. Likely should be X-ray Photoelectron Spectroscopy
(XPS) aka Electron Spectrscopy for Chemical Analysis (ESCA)

Excellent surface analysis technique for determining elemental
concentrations of surfaces (top few atomic layers) and some information on
chemical bonding environment. Many applications in polymer science,
microelectronic manufacturing, corrosion, and more. (I'm running some
samples this afternoon!)


For introductions explore the following web sites:
http://www.uksaf.org/home.html
http://www.uwo.ca/ssw/


There are many books; here are a few from my shelf:
Practical Surface Analysis, 2nd ed., Vol 1--Auger and X-ray Photoelectron
Spectroscopy. Edited by D. Briggs & M. P. Seah
Modern ESCA: The Principles and Practice of X-Ray Photoelectron
Spectroscopy. Tery L. Barr
Surface Analysis: The Principle Techniques. Edited by John C. Vickerman


Hope this helps!

Jim Passmore
Sr. Analytical Chemist
Cryovac Division, Sealed Air Corporation
Duncan, SC







"jshields-at-cb.u
ga.edu" To: Microscopy-at-sparc5.microscopy.com
cc:
05-23-02 09:36 Subject: x-ray photoctron?
AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi All,
Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
please provide brief description and some citations.
Thanks!

John Shields
EM Lab
Univ. of Georgia
Athens, GA 30602







From daemon Thu May 23 13:43:20 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 23 May 2002 11:52:43 -0700
Subject: Re: diamond knife on cpd. microscope

Contents Retrieved from Microscopy Listserver Archives
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Dear Rosemary
It's out of topic, but it seems to me, people use lasers for such
purpose. You could direct laser using the same lens you are using for
observation and use it as a 'laser blade'. I kind of pessimistic how you
could use proposed tool with x100 lens: distance between lens and sample is
too small. I know tnat our Medical School do have commercial apparatus for
such purpose. I believe it's Olympus (very expensive). Sergey

At 10:47 AM 5/23/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu May 23 13:44:08 2002



From: tbargar-at-unmc.edu
Date: Thu, 23 May 2002 13:02:04 -0500
Subject: Digital camera for photomicrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Is it possible to buy a digital camera that can be used as a camera and by
using an adaptor also be mounted on any light microscope for use in
photomicrography? I've been asked by my dept. chairman to gather
information. His goal, a departmental camera for everyone to use. As
regards the light photomicrography it would be bright field and
fluorescence. Any information will be appreciated, I'm a complete newbie
in this digital stuff.

Tom Bargar
CEMRF-Univ. of Neb. Med. Ctr.
tbargar-at-unmc.edu
402-559-7347




From daemon Thu May 23 14:10:48 2002



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Thu, 23 May 2002 15:04:28 -0400
Subject: x-ray photoctron?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John,
Sounds like a typo that should read x-ray photoelectron
spectroscopy. A web search for "XPS" or "ESCA" (electron spectroscopy for
chemical analysis) should give you all the info you need.
In brief, monochromatic soft x-rays (typically Mgka 1253.6eV or AlKa
1486.6eV) are used to photo-emit electrons from core atomic levels of
elements in a solid sample in high vacuum. The photoelectrons are energy
analyzed to give a spectrum which uniquely defines the elements present in
the sample (except H or He).
Subtle shifts in peak position for each element gives information on
the bonding of that element within the sample (hence "chemical analysis").
Another major advantage of the technique is that it is extremely
surface sensitive. Only the top ~100 Angstroms of the sample are analyzed
because although the x-rays penetrate much deeper the mean free path of
electrons of energy ~100eV-1486eV in a solid is typically {50A so only
electrons in the near-surface region can escape the material without
scattering.

All the Best,

Steve

Steve Buckingham
Excellatron Solid State LLC.
1640 Roswell St, Suite J.
Smyrna, GA 30080
Ph: 770 438 2201
Fax: 617 812 5920
sbuckingham-at-excellatron.com {mailto:sbuckingham-at-excellatron.com}


-----Original Message-----
} From: "jshields-at-cb.uga.edu"-at-sparc5.microscopy.com
[mailto:"jshields-at-cb.uga.edu"-at-sparc5.microscopy.com]
Sent: Thursday, May 23, 2002 9:37 AM
To: Microscopy-at-sparc5.microscopy.com


Hi All,
Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
please provide brief description and some citations.
Thanks!

John Shields
EM Lab
Univ. of Georgia
Athens, GA 30602



From daemon Thu May 23 14:30:12 2002



From: Carlton, Robert :      robert.carlton-at-elan.com
Date: Thu, 23 May 2002 15:24:25 -0400
Subject: Philadelphia Society of Microscopy Meeting at Longwood Gardens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



SPECIAL MEETING:

Thursday, June 6th

"Research At Longwood Gardens -
In search of the perfect horticultural display"

Jim Harbage,
Plant Physiologist/Propagator, Longwood Gardens

To be held at:
Longwood Gardens, Kennett Square PA


Please see the Announcements Section for important information regarding
electronic copies of this notice!
Meeting Details

Location: LONGWOOD GARDENS, Kennett Square PA
Schedule: 4:30 - 5:30: Guided tour of including the
production greenhouse and (hopefully) the pest management lab.
6:00 Buffet dinner at the Terrace Restaurant
7:00 Talk
Directions: Can be found at http://www.longwoodgardens.org/
Cost: Entrance to the park is $12. We are also collecting $10 to
help defray the cost of dinner.
For those who want to take the tour, we will be gathering at the
Conservatory main entrance at 4:30 (I know that's too early for some, but
that's the best we can do). For everyone else, we will have a table set up
at the Terrace Restaurant.
The talk, at 7:00, will be be presented at the Terrace Restaurant.
Reservations
Reservations required. We have been asked to give Longwood Gardens an idea
of how many people will attend. Please let me know if you will be attending
the tour or just the dinner and talk.
By E-Mail (preferred): Send your name and affiliation to
jrreffner-at-rohmhaas.com
By Phone: Call John Reffner, 215-619-5283
DEADLINE Monday, June 3th. I will confirm all e-mail reservations

About the Speaker

Jim Harbage is Plant Physiologist/Propagator and I work in the Research
Division at Longwood Gardens, Inc. He has been there about 2.5 years.
Prior to that he was an Associate Professor of Horticulture at South Dakota
State University for 6 years. He hold a BSc and MSc from University of
Maryland - College Park and a Ph.D from University of Wisconsin - Madison.
All are with majors in horticulture. His primary focus areas at Longwood
include conduct plant trials and plant improvement programs, optimize plant
culture, utilize tissue culture methods for propagation and virus
elimination.

Other Announcements
IMPORTANT NOTICE REGARDING EMAIL:
It is important that PSM has your email address. We are considering
switching our meeting notifications to email ONLY. This would save the
society some money and significantly reduce the work of the executive
committee. Also, and most importantly, we could communicate more often and
give members more advanced notice of upcoming events.

EASTERN ANALYTICAL SYMPOSIUM:IMPORTANT ANNOUNCEMENT
The Eastern Analytical Symposium will return to its Somerset, New Jersey
location starting with the 2002 Meeting
The Symposium Dates will be November 18-21, 2002 Garden State Exhibit
Center, Somerset, New Jersey

Philadelphia Society for Microscopy Executive Council, 2001
President Past President President-Elect Secretary-Treasurer and
Newsletter Editor Corporate Liaison
Robert Carlton Pat Connelly Robert Carlton John Reffner Open
(610)- 313-1360 (215)- 898-7145 (610)- 313-1360 (215) 619-5283



Robert A. Carlton
Elan Drug Delivery, Inc.
3500 Horizon Drive
King of Prussia, PA, 19406
610-313-1360
robert.carlton-at-elan.com



********************************************************************
This communication and any files transmitted with it
may contain information that is confidential, privileged
and exempt from disclosure under applicable law.
It is intended solely for the use of the individual or entity
to which it is addressed. If you are not the intended
recipient, you are hereby notified that any use,
dissemination or copying of this communication is strictly
prohibited. If you have received this communication in
error, please notify the sender. Thank you for your co-operation.
********************************************************************



From daemon Thu May 23 16:13:05 2002



From: Anne-Marie Brun :      abrun-at-hsc.unt.edu
Date: Thu, 23 May 2002 16:04:53 -0500
Subject: Microscopy user fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all of you who responded to my email. You are a real help.
My new question now to you is: if you charge all that money, how fast is
your turn around time of samples? Would you please mention how many
people work in the lab, who actually do the work.
Thanks
Anne-Marie Brun-Zinkernagel



From daemon Thu May 23 16:38:09 2002



From: Richard Harris :      rjharris-at-uwo.ca
Date: Thu, 23 May 2002 17:38:48 -0400
Subject: Fitting a digital video camera to a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers;
Does anyone have suggestions on how to fit or couple a retail (like a Sony
or Cannon) digital video camera to a microscope? Are there any commercial
products to do this? I've been asked by a Prof who'd like to (on the cheap)
capture the activities of microscopic marine invertebrates for later editing
and compiling on his desktop computer. He has a Sony digital camcorder and
Apple G4 system. I'd really appreciate your feedback.

Thanks;
Richard Harris
Laboratory Supervisor
Imaging and Analysis
Department of Zoology
University of Western Ontario
London ON
CANADA N6A 5B7
(519) 661-2111 ext 86780
(519) 661-2014 Fax




From daemon Thu May 23 22:16:27 2002



From: RCHIOVETTI-at-aol.com
Date: Thu, 23 May 2002 23:07:26 EDT
Subject: Re: diamond knife on cpd. microscope

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 05/23/2002 7:07:02 AM US Mountain Standard Time,
rw9-at-psu.edu writes:

{ { "a diamond knife that can be installed onto a compound microscope
like a lens so that it can be used to cut a tiny circle on a plastic
membrane on a glass slide. One would look for a particular cell or
tissue with the desired properties using a regular lens (40X or
100X), and then switch to the knife, which is just a little off
center, and turn the knife around the central axis to score the
around the sample. A little buffer would then float the cut sample,
which can then be picked up by a TEM grid for observation using TEM."
} }

Rosemary,

Most microscope manufacturers can provide a diamond slide marker or diamond
scribe that's used to mark slides...I suppose you could use it to scribe a
plastic section and then float off the scribed area.

The only difficulty would be a lack of control of the circle's size. You
could only scribe one size of circle, whatever the scribe comes equipped to
do. This would make it rather difficult to excise single cells if they are
too close together.

You might want to check out the Eppendorf website. They make a micro-chisel
that's designed for this type of thing. It mounts on the end of a
micromanipulator and also has a micropipette for flushing the chiseled-out
cell and collecting the material. Go to {www.eppendorf.com} and follow the
links: Products -} Cell Technology -} Microdissector.

You can also check with the microscope manufacturers for specialized
instruments that can do the same thing: laser microdissection (Leica), laser
capture (Arcturus), laser catapulting (Zeiss/PALM), etc. Very cool
technology, but also very pricey.

It would probably be quite difficult to pick up the excised single cell in
plastic and transfer to an EM grid, but it might be worth a try. I suppose
you could use either the Leica laser microdissection system of the PALM unit,
since they are non-contact methods, and collect the excised specimen onto a
drop of buffer.

The Arcturus probably wouldn't be suitable, since it depends on heating a
transfer film to make it sticky and then adhering the tissue or specimen to
the film.

Maybe there's a core lab or a research pathology lab in your area that has
one of the above instruments??

Good luck!

Cheers,

Bob Chiovetti


From daemon Thu May 23 23:28:27 2002



From: Edward_Principe-at-amat.com
Date: Thu, 23 May 2002 21:20:58 -0700
Subject: Re: diamond knife on cpd. microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Rosemary,

Could a dual beam FIB be used to cut your "tiny circle" ? How thick is the
plastic?

-Ed




Sergey Ryazantsev {sryazant-at-ucla.edu} on 05/23/2002 11:52:43 AM

To: Rosemary Walsh {rw9-at-psu.edu} , microscopy-at-sparc5.microscopy.com
cc:


Dear Rosemary
It's out of topic, but it seems to me, people use lasers for such
purpose. You could direct laser using the same lens you are using for
observation and use it as a 'laser blade'. I kind of pessimistic how you
could use proposed tool with x100 lens: distance between lens and sample is
too small. I know tnat our Medical School do have commercial apparatus for
such purpose. I believe it's Olympus (very expensive). Sergey

At 10:47 AM 5/23/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America








From daemon Fri May 24 00:28:41 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Fri, 24 May 2002 07:20:40 +0200
Subject: Moth Prep

Contents Retrieved from Microscopy Listserver Archives
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Dear Barbara

If you still run into charging problems on your moth try a thin Carbon coat
under high vacuum prior to a AuPd coat. Rotary coating in the high vacuum
will be preferred during the carbon evaporation.
If you are fortunate to have a ESEM AuPd coating will not be needed. We had
some nice fun here and were rewarded with interesting pics ;-)

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana
{ {...OLE_Obj...} }

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}




From daemon Fri May 24 06:34:18 2002



From: Philip Koeck :      Philip.Koeck-at-biosci.ki.se
Date: Fri, 24 May 2002 13:33:00 +0200
Subject: objective aperture position

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I'm wondering whether our objective aperture is correctly positioned (or the
column
correctly aligned).
If I focus a diffusely scattering specimen at the eucentric height and then
switch to diffraction
mode with a relatively parallel beam (the beam covers the entire screen at a
magnification of
about 30K) I can't get a sharp (minimal) central spot and a sharp image of
the aperture
at the same time.
Is there something wrong with our microscope or with my idea of electron
optics?

sincerely,


Philip Koeck
Svdertvrns Hvgskola and
Karolinska Institutet
Dept. of Bioscience at Novum
S-14157 Huddinge
Sweden
phone: +46-8-6089186
fax: +46-8-6089290
http://www.biosci.ki.se/em

The phrase 'We have always done things this way.'
is as much a reason to change as a reason not to.
- Dartwill Aquila
_______________________________________



From daemon Fri May 24 06:57:02 2002



From: Dmrelion-at-aol.com
Date: Fri, 24 May 2002 07:50:36 EDT
Subject: Re: Fitting a digital video camera to a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard, Diagnostics Instrument, Inc. has a nice catalog of couplers for
all sorts of microscopes and all sorts of cameras. info-at-diaginc.com and
www.diaginc.com. I have no commercial interest in the company but have used
their products.

Don Marshall
RELION Industries
cathodoluminescence microscopy
mass spectroscopy
electron beam technology
PO Box 12
Bedford, MA 01730

781-275-4695 (Phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

http://www.excitingelectrons.com



In a message dated 05/24/2002 4:11:10 AM Eastern Daylight Time,
rjharris-at-uwo.ca writes:

{ { Subj: Fitting a digital video camera to a microscope
Date: 05/24/2002 4:11:10 AM Eastern Daylight Time
From: rjharris-at-uwo.ca (Richard Harris)
Reply-to: rjharris-at-uwo.ca
To:

(Microscopy Listserver)



Dear Listers;
Does anyone have suggestions on how to fit or couple a retail (like a Sony
or Cannon) digital video camera to a microscope? Are there any commercial
products to do this? I've been asked by a Prof who'd like to (on the cheap)
capture the activities of microscopic marine invertebrates for later editing
and compiling on his desktop computer. He has a Sony digital camcorder and
Apple G4 system. I'd really appreciate your feedback.

Thanks;
Richard Harris



From daemon Fri May 24 07:53:39 2002



From: John W. Raffensperger, Jr. :      johnr-at-helwigcp.com
Date: Fri, 24 May 2002 07:48:10 -0500
Subject: FW: Fitting a digital video camera to a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard and Tom;

(Tom, I'm assuming that you have a sub $1,000 digital camera in mind, as
opposed to a full blown SLR type digital.)

I have coupled both a Sony still, and a Sony Camcorder to a light
microscope. There is a commercial adapter that can be found at:

http://www.lensadapter.com

In looking at this online, I noticed that there were no optics, and that
it was just an adapter tube. As a quick test, I took a number of
cameras from both work and home, and just held them to the eyepiece, and
lo, there was a useable image.

For the small and light still camera I have, I machined an adapter with
a slight press fit over the camera lens barrel, and a slip fit over the
microscope eyepiece. This works great. The machining needs to be close
on the fits, as any "wobble" results in vignetting of the image.

For the heavier still cameras and camcorders, I've used a tripod to
support the weight of the camera, but am working on an easier to adjust
stand (X, Y, Z adjustments) to align and support the camera.

The only real issue I've run into is focusing. For the most part, the
cameras auto focus works well for the camera itself, but in my case the
focus of the viewing tube and camera tube are not always exactly the
same. Trying to focus via the digital cameras LCD screen is less than
optimal, as the resolution is too low. You kind of have to go for the
"least blurry" image.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Richard Harris [mailto:rjharris-at-uwo.ca]
Sent: Thursday, May 23, 2002 4:39 PM

Dear Listers;
Does anyone have suggestions on how to fit or couple a retail (like a
Sony
or Cannon) digital video camera to a microscope? Are there any
commercial
products to do this? I've been asked by a Prof who'd like to (on the
cheap)
capture the activities of microscopic marine invertebrates for later
editing
and compiling on his desktop computer. He has a Sony digital camcorder
and
Apple G4 system. I'd really appreciate your feedback.

Thanks;
Richard Harris
Laboratory Supervisor
Imaging and Analysis
Department of Zoology
University of Western Ontario
London ON
CANADA N6A 5B7
(519) 661-2111 ext 86780
(519) 661-2014 Fax

-----Original Message-----
} From: "tbargar-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"tbargar-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Thursday, May 23, 2002 1:02 PM

Is it possible to buy a digital camera that can be used as a camera and
by
using an adaptor also be mounted on any light microscope for use in
photomicrography? I've been asked by my dept. chairman to gather
information. His goal, a departmental camera for everyone to use. As
regards the light photomicrography it would be bright field and
fluorescence. Any information will be appreciated, I'm a complete
newbie
in this digital stuff.

Tom Bargar
CEMRF-Univ. of Neb. Med. Ctr.
tbargar-at-unmc.edu
402-559-7347



From daemon Fri May 24 08:02:34 2002



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Fri, 24 May 2002 08:56:21 -0400
Subject: Re: objective aperture position

Contents Retrieved from Microscopy Listserver Archives
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Philip and others,

The problem you face comes from the use of immersion lenses in the
modern TEM. The illumination may not be parallel simply because you
spread the beam.

Here are the rules: The true back focal plane can be found by looking
at the Kikuchi lines. When the Kikuchi lines are brought to best
sharpness with the diffraction focus control, you are looking at the
back focal plane. Make this adjustment with the illumination focused at
the specimen plane. If you put the objective aperture in now, it should
give a sharp shadow. If it does not, depending on the model of
microscope that you use, it may be appropriate to adjust the height of
the aperture. If you have the diffraction focus set this way, the beam
will be "parallel" when the spots are sharp.

On the other hand there is no good reason, most of the time, to work in
the back focal plane. Instead, spread the beam to cover the area you
want and make the spots sharp with the diffraction focus. The only
drawback with this is that the camera length is not calibrated.

Here are two publications that may (or may not) help.

'Skew Thoughts on Parallelism'
K. K. Christenson and J. A. Eades
Ultramicroscopy 26 (1988) 113-132

Topics in Electron Diffraction (TEM): A Tutorial
J. A. Eades
Microscopy and Microanalysis 7 Suppl 2 (2001) 764-765


Philip Koeck wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
}
} I'm wondering whether our objective aperture is correctly positioned (or the
} column
} correctly aligned).
} If I focus a diffusely scattering specimen at the eucentric height and then
} switch to diffraction
} mode with a relatively parallel beam (the beam covers the entire screen at a
} magnification of
} about 30K) I can't get a sharp (minimal) central spot and a sharp image of
} the aperture
} at the same time.
} Is there something wrong with our microscope or with my idea of electron
} optics?
}
} sincerely,
}
} Philip Koeck
} Svdertvrns Hvgskola and
} Karolinska Institutet
} Dept. of Bioscience at Novum
} S-14157 Huddinge
} Sweden
} phone: +46-8-6089186
} fax: +46-8-6089290
} http://www.biosci.ki.se/em
}
} The phrase 'We have always done things this way.'
} is as much a reason to change as a reason not to.
} - Dartwill Aquila
} _______________________________________

--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Fri May 24 09:26:23 2002



From: jshields-at-cb.uga.edu
Date: Fri, 24 May 2002 10:09:51 -0400
Subject: photoctron corrected

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks all for the input.
} I figured it must be a typo for photoelectron, but wanted
} to make sure I wasn't missing some *new*
} technique/equipment out there... You never know these
} days. john


From daemon Fri May 24 09:26:24 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Fri, 24 May 2002 11:12:12 -0500
Subject: responses on post: diamond knife on cpd. microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,
Once again --thanks to all who responded. Here are the
responses to date:
Tom McGill, Leica
I saw your posting on the listserver just before I left for the day and
thought I would send you a quick email. What you are describing sounds a lot
like a slide marker or object marker.
I am a sales rep for Leica here in Toronto. We have a part # 11505059 and
this is called "Object Marker with thread M25"
It is not a knife per se, but it is a sharp diamond tip that scribes a
circular scratch into the coverslip of a normal slide. You find the area of
interest with the 40x, then swing in the diamond marker, pull down and
rotate the collar, thus marking the coverslip. Take the slide off of the
scope, then you rub India Ink over this scratch.... the ink goes into the
circle and leaves a nice circle so that interesting parts of the slide can
be high-lighted.... similar to the marks that cytotechs put on the slides so
that cytopathologists will know where to look.

Patty Jansma { Neurobiology, Arizona
I haven't heard of exactly what you are looking for but I have seen a
Zeiss "marker" lens similiar to what you are describing. You can flat
embed your cells and find the cell under the compound scope. After that
you rotate in the marker lens and cut the circle around the area of
interest. The difference is that you can remount this area and then
section it.

Sergey Ryazantsev, UCLA
It's out of topic, but it seems to me, people use lasers for such
purpose. You could direct laser using the same lens you are using
for observation and use it as a 'laser blade'. I kind of pessimistic
how you could use proposed tool with x100 lens: distance between lens
and sample is too small. I know tnat our Medical School do have
commercial apparatus for such purpose. I believe it's Olympus (very
expensive).

John Reber, Zeiss
Carl zeiss has a Diamond marker, 462960, $1,385.00. It screws into a standard
microscope nosepiece. The diameter of the circle may be set with a graduated
scale. Engraving takes place by pulling down the spring loaded diamond and
turning the knurled ring.
Regards, John Reber


Gretchen Ziegler, Leica
I believe our company, Leica Microsystems, can do this for you. I will
have a rep get in contact with you.

Chuck Garber, SPI Supplies
For your information we have sold some of our diamond scalpel blades, see
SPI's URL for similar soundingapplications.
However, one has to be really really careful about not damaging the end.
This is something that has happened, and has resulted in at least one
relatively unhappy customer. I don't know what more I could say about this
except I don't see how you could do this with the use of anything else.
Chuck, SPI Supplies



Bob Chiovetti
Most microscope manufacturers can provide a diamond slide marker or diamond
scribe that's used to mark slides...I suppose you could use it to scribe a
plastic section and then float off the scribed area.

The only difficulty would be a lack of control of the circle's size. You
could only scribe one size of circle, whatever the scribe comes equipped to
do. This would make it rather difficult to excise single cells if they are
too close together.

You might want to check out the Eppendorf website. They make a micro-chisel
that's designed for this type of thing. It mounts on the end of a
micromanipulator and also has a micropipette for flushing the chiseled-out
cell and collecting the material. Go to {www.eppendorf.com} and follow the
links: Products -} Cell Technology -} Microdissector.
You can also check with the microscope manufacturers for specialized
instruments that can do the same thing: laser microdissection (Leica), laser
capture (Arcturus), laser catapulting (Zeiss/PALM), etc. Very cool
technology, but also very pricey.
It would probably be quite difficult to pick up the excised single cell in
plastic and transfer to an EM grid, but it might be worth a try. I suppose
you could use either the Leica laser microdissection system of the PALM unit,
since they are non-contact methods, and collect the excised specimen onto a
drop of buffer.
The Arcturus probably wouldn't be suitable, since it depends on heating a
transfer film to make it sticky and then adhering the tissue or specimen to
the film. Good luck! Bob Chiovetti


Edward Principe
Could a dual beam FIB be used to cut your "tiny circle" ? How thick is the
plastic?

Rosemary






--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Fri May 24 09:26:58 2002



From: Margaret Miller :      MILLERMM-at-uthscsa.edu
Date: Fri, 24 May 2002 08:22:34 -0500
Subject: Fees for clinical services

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--
Dear listers,

If there is anyone who doesn't mind sharing their fee
structure I would like to know what other electron microscopy
facilities are charging for clinical services. With and without
the professional component.

As always,
Thanks for your input,
Peggy Miller


From daemon Fri May 24 09:56:34 2002



From: dale anderson :      dale.anderson-at-attglobal.net
Date: Fri, 24 May 2002 10:49:39 -0400
Subject: Fitting a digital video camera to a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The May issue of the MSA magazine "Microscopy Today" has a solid article
by Ted Clarke on fitting a retail digital camera to a microscope column.
In it he cites several equally useful articles on the subject. Richard
Harris: if you don't get Microscopy Today, send me a note and I'll send
you a copy of the May issue. You can subscribe at
www.microscopy-today.com.

Ron Anderson, MT Editor

-----Original Message-----
} From: Richard Harris [mailto:rjharris-at-uwo.ca]
Sent: Thursday, May 23, 2002 5:39 PM
To: Microscopy Listserver


Dear Listers;
Does anyone have suggestions on how to fit or couple a retail (like a
Sony
or Cannon) digital video camera to a microscope? Are there any
commercial
products to do this? I've been asked by a Prof who'd like to (on the
cheap)
capture the activities of microscopic marine invertebrates for later
editing
and compiling on his desktop computer. He has a Sony digital camcorder
and
Apple G4 system. I'd really appreciate your feedback.

Thanks;
Richard Harris
Laboratory Supervisor
Imaging and Analysis
Department of Zoology
University of Western Ontario
London ON
CANADA N6A 5B7
(519) 661-2111 ext 86780
(519) 661-2014 Fax






From daemon Fri May 24 15:05:35 2002



From: Jim Haley :      haley-at-mvia.com
Date: Fri, 24 May 2002 15:57:09 -0700
Subject: Re: Fitting a digital video camera to a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard,

We have a universal adapter that will mount the camera on a microscope
trinocular head. This is much better than mounting on the eyepieces
because the camera is more stable. The adapter can screw onto the front
end of most cameras (some will require a step down ring).

It also has a parfocalization adjustment which will allow you to get the
camera and eyepieces in focus at the same time on the microscope.

Please feel free to email me or call if you have any questions or would
like additional information.

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
12901 Duckettown Road
Laurel, MD 20708
voice: (301) 809-6292
fax: (603) 676-2966
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

Richard Harris wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers;
} Does anyone have suggestions on how to fit or couple a retail (like a Sony
} or Cannon) digital video camera to a microscope? Are there any commercial
} products to do this? I've been asked by a Prof who'd like to (on the cheap)
} capture the activities of microscopic marine invertebrates for later editing
} and compiling on his desktop computer. He has a Sony digital camcorder and
} Apple G4 system. I'd really appreciate your feedback.
}
} Thanks;
} Richard Harris
} Laboratory Supervisor
} Imaging and Analysis
} Department of Zoology
} University of Western Ontario
} London ON
} CANADA N6A 5B7
} (519) 661-2111 ext 86780
} (519) 661-2014 Fax

--


From daemon Fri May 24 15:06:43 2002



From: Weiskittel, Amy L. :      Amy.Weiskittel-at-Equistarchem.com
Date: Fri, 24 May 2002 15:00:51 -0500
Subject: Need help with support medias for air sensitive catalysts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in speaking with someone about support medias (i.e. epoxies, etc.) for moisture and/or oxygen sensitive catalysts
that would allow me to cross-section the catalyst for elemental analysis.


From daemon Fri May 24 18:34:57 2002



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Fri, 24 May 2002 16:26:31 -0700
Subject: Need help with support medias for air sensitive catalysts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Amy,

to prevent the oxygen exposure, we do the processing and sectioning of
O2-sensitive samples in anaerobic glove bags (Coit, Inc.). We take
advantage of LR White resin properties to polymerize easily at anaerobic
conditions; I prefer the hard grade. I like to complain about the challenge
of anaerobic microtomy (the microtome is placed in a glove bag, and the
controls, as well as the binoculars, are accessed through the gloves /
plastic wall you press your nose against). However, we found out that even
those 15 minutes needed for thin sectioning can make a difference in oxygen
sensitive material chemistry. I guess there is no way to prevent the brief
period in room atmosphere while transferring the grid into the holder. I
transport the grid from the glove box to the TEM room in a sealed container.
Assuming there is no significant oxidation in the TEM vacuum. Make sure all
your processing reagents are anoxic (purged with a gas), as well as the
water in your knife.
Feel free to contact me for more info.
Alice.

Alice Dohnalkova
Environmental Microbiology
Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692 office
(509) 376-3654 TEM lab
fax (509) 376-1321

-----Original Message-----
} From: Weiskittel, Amy L. [SMTP:Amy.Weiskittel-at-Equistarchem.com]
Sent: Friday, May 24, 2002 1:01 PM
To: Microscopy-at-sparc5.microscopy.com


I am interested in speaking with someone about support medias (i.e. epoxies,
etc.) for moisture and/or oxygen sensitive catalysts
that would allow me to cross-section the catalyst for elemental analysis.


From daemon Fri May 24 22:28:38 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Thu, 23 May 2002 23:16:21 -0500
Subject: LM stain for phenolics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What is the best LM staining technique for phenolics in dense hardwoods (core
samples and sledge microtome cut sections)?




From daemon Sat May 25 03:47:58 2002



From: mohammed y abdulrawoof / f40z006 75760 :      mdyousuf-at-KSU.EDU.SA
Date: Sat, 25 May 2002 09:16:42 -0300 (GMT)
Subject: Osmium Tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Greetings of the day.
During a cleanup of our chemical stores I found a package of 1 gm ampules
of Osmium Tetroxide crystals in a large number (presumably they have been
there for over a decade plus). My question is; what is the shelf life
period of these ampules (refrigerated or unrefrigerated). Can they be used
now reliably to make a fixative solution?

A colleague told me that he used one of them in recent times and
complained that as soon as the crystals dissolve in buffer or distilled
water, the solution turns dark immediately (rather than after a prolonged
period)

Regards to all.

M. Yousuf Abdul-Rawoof
Department of Zoology
College of Science
King Saud University
POB 2455, Riyadh 11451
Saudi Arabia

mdyousuf-at-ksu.edu.sa







From daemon Sat May 25 10:48:21 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 25 May 2002 08:32:24 -0700
Subject: Re: Need help with support medias for air sensitive catalysts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I am interested in speaking with someone about support medias (i.e.
} epoxies, etc.) for moisture and/or oxygen sensitive catalysts
} that would allow me to cross-section the catalyst for elemental analysis.

Amy -

Acrylic (LR White, hard) works for some catalysts, epoxy for others; you
can start here:

Csencsits, R., Schooley, C., and Gronsky, R. (1985) An improved method
for thin sectioning of particulate catalysts. J.E.M. Technique 2:643-644.

Ulan, J.G., Schooley, C., & Gronsky, R. (1990) Microtomy of large
particle zeolites for TEM. Mat. Res. Soc. Symp. Proc. 199:153-156

Ulan, J.G., Schooley, C., & Gronsky, R. (1990) Modified embedment
procedure for microtomy of large particle zeolites. J.E.M. Technique
16:254-255


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun May 26 12:18:31 2002



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 5/25/02 9:16 AM
Subject: Osmium Tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

In the past, a retiring professor donated some 100 1 gram vials that
had been stored for years in his attic, with the attendant harsh
swings in seasonal temperatures in such an environment. The osmium
worked well as I remember. Sure wouldn't hurt to try some of the
vials out and see what happens in water, then buffer. Leave critical
applications for OsO4 that you trust.

Chuck Butterick
Degussa Corporation
Borger, Texas


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers,
Greetings of the day.
During a cleanup of our chemical stores I found a package of 1 gm ampules
of Osmium Tetroxide crystals in a large number (presumably they have been
there for over a decade plus). My question is; what is the shelf life
period of these ampules (refrigerated or unrefrigerated). Can they be used
now reliably to make a fixative solution?

A colleague told me that he used one of them in recent times and
complained that as soon as the crystals dissolve in buffer or distilled
water, the solution turns dark immediately (rather than after a prolonged
period)

Regards to all.

M. Yousuf Abdul-Rawoof
Department of Zoology
College of Science
King Saud University
POB 2455, Riyadh 11451
Saudi Arabia

mdyousuf-at-ksu.edu.sa










From daemon Sun May 26 17:41:12 2002



From: Philippe A. Buffat :      philippe.buffat-at-epfl.ch
Date: Sun, 26 May 2002 20:15:52 -0700
Subject: Re: objective aperture position

Contents Retrieved from Microscopy Listserver Archives
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X-Sender: pbuffat-at-imap.epfl.ch
X-Mailer: QUALCOMM Windows Eudora Version 5.1


Philip, Alwyn and others,

I fully agree with Alwyn comments as long as you deal with diffraction. You
just need to recalibrate more often your camera length.

But the "reciprocal issue" that is not solved on modern microscopes with
"twin" lenses or equivalent short focus is the unability to put the
diffraction aperture in hte back focal plane of the objective because of
geometrical constraintgs.

It means that it is not possible to accurately select a diffraction spot,
i.e. to have a true bright field or a clean dark field from a
superstructure reflection.

Moreover at low mag. the shadow of the objective aperture covers the field
of view (it happen up to 100'000x for those tempted by short Cs lenses!).

Only solution: travel to whom has still this good old JEOL 200CX (or buy a
"biologist microscope" with long focal lengths/weak objective lenses if you
agree with 100 kV.

Regards

Philippe Buffat

At 08:56 24.05.2002, Alwyn Eades wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



_______________________________________________________________
Philippe A. Buffat
Ecole Polytechnique Federale de Lausanne (EPFL)
Centre Interdisciplinaire de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/


***NEW*** European Cells and Materials: Free, on-line, peer reviewed journal
http://www.eurocellmat.org.uk/
_______________________________________________________________




From daemon Mon May 27 04:34:12 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 27 May 2002 10:12:36 +0100 (GMT Daylight Time)
Subject: Re: Osmium Tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have read on the listserver that discoloured osmium
tetroxide can be regenerated by adding (I think - please
check archives!) hydrogen peroxide.

Dave


On Sun, 26 May 2002 11:50:14 -0500 Chuck Butterick
{cbutte-at-ameripol.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers,
}
} In the past, a retiring professor donated some 100 1 gram vials that
} had been stored for years in his attic, with the attendant harsh
} swings in seasonal temperatures in such an environment. The osmium
} worked well as I remember. Sure wouldn't hurt to try some of the
} vials out and see what happens in water, then buffer. Leave critical
} applications for OsO4 that you trust.
}
} Chuck Butterick
} Degussa Corporation
} Borger, Texas
}
}
} ______________________________ Reply Separator _________________________________
} Subject: Osmium Tetroxide
} Author: mohammed y abdulrawoof / f40z006 75760 {mdyousuf-at-KSU.EDU.SA} at
} INTERNET-MAIL
} Date: 5/25/02 9:16 AM
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} Greetings of the day.
} During a cleanup of our chemical stores I found a package of 1 gm ampules
} of Osmium Tetroxide crystals in a large number (presumably they have been
} there for over a decade plus). My question is; what is the shelf life
} period of these ampules (refrigerated or unrefrigerated). Can they be used
} now reliably to make a fixative solution?
}
} A colleague told me that he used one of them in recent times and
} complained that as soon as the crystals dissolve in buffer or distilled
} water, the solution turns dark immediately (rather than after a prolonged
} period)
}
} Regards to all.
}
} M. Yousuf Abdul-Rawoof
} Department of Zoology
} College of Science
} King Saud University
} POB 2455, Riyadh 11451
} Saudi Arabia
}
} mdyousuf-at-ksu.edu.sa
}
}
}
}
}
}
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon May 27 07:18:05 2002



From: Ann-Fook Yang :      yanga-at-EM.AGR.CA
Date: Mon, 27 May 2002 08:09:16 -0400
Subject: Re: Osmium Tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think it was the buffer or bottle that contained organic matter causing osmium solution to turn black. My osmium crystal had been purchased several decades ago and there is no problem.

Ann Fook

} } } mohammed y abdulrawoof / f40z006 75760 {mdyousuf-at-KSU.EDU.SA} 05/25/02 08:16AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers,
Greetings of the day.
During a cleanup of our chemical stores I found a package of 1 gm ampules
of Osmium Tetroxide crystals in a large number (presumably they have been
there for over a decade plus). My question is; what is the shelf life
period of these ampules (refrigerated or unrefrigerated). Can they be used
now reliably to make a fixative solution?

A colleague told me that he used one of them in recent times and
complained that as soon as the crystals dissolve in buffer or distilled
water, the solution turns dark immediately (rather than after a prolonged
period)

Regards to all.

M. Yousuf Abdul-Rawoof
Department of Zoology
College of Science
King Saud University
POB 2455, Riyadh 11451
Saudi Arabia

mdyousuf-at-ksu.edu.sa









From daemon Mon May 27 12:03:19 2002



From: Rik Brydson :      mtlrmdb-at-leeds.ac.uk
Date: Mon, 27 May 2002 17:39:56 +0100
Subject: FW: Second SuperSTEM workshop - 11/7/02 London

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SuperSTEM ­ the Second Workshop
Thursday 11th July 2002
part of RMS Microscience 2002, ExCel, London.

The UK SuperSTEM project aims to develop a UK national facility comprising a
suite of ultra high resolution imaging and analytical scanning transmission
electron microscopes (STEMs) at Daresbury Laboratories in Cheshire. The
principle of SuperSTEM is based on the computer controlled aberration
correction of a STEM objective lens using quadrupole and octupole lenses.
This allows the formation of a sub-Angstrom probe for simultaneous ultra
-high resolution high angle annular dark field (HAADF) imaging and
atomic-column electron energy loss spectroscopy (EELS) analysis of thin
specimens.

Following the success of the first SuperSTEM User Workshop at the EMAG 2001
conference at Dundee, the second workshop will be held as part of RMS
Microscience 2002 at the ExCel centre in London and will present the first
results from SuperSTEM I - the first aberration corrected machine which is
currently being tested at Cambridge prior to being moved to a purpose built
facility at Daresbury Laboratories in September 2002. Speakers include Dr
Pete Nellist from NION inc., USA and Dr Andrew Bleloch, technical director
of the SuperSTEM project. We encourage all interested parties to attend.

If you are interested in reserving a place on the workshop then please
email: abeckerl-at-liverpool.ac.uk or write to Mrs A Beckerlegge, SuperSTEM
Secretary, Materials Science and Engineering, University of Liverpool,
Ashton Building, Liverpool L69 3GH, UK.

The registration costs for this second workshop are £25 for the day ­ please
register with the Royal Microscopical Society
(http://www.microscience2002.org.uk). In addition to the workshop there will
also be other scientific sessions during the day (e.g. energy filtered TEM
imaging, focussed ion beam microscopy, variable pressure SEM and electron
backscattered diffraction) as well as Europe¹s largest exhibition of
microscopes and scientific equipment. Further details can be obtained from
the Microscience website or by emailing info-at-rms.org.uk.



--
_____________________________
Dr. Rik Brydson,
Leeds Electron Microscopy and Spectroscopy (LEMAS) Centre
Department of Materials,
University of Leeds,
Leeds LS2 9JT, U.K.

Tel: 44 + (0)113 343 2369
Fax: 44 + (0)113 242 2531
Web: http://www.materials.leeds.ac.uk
Web: http://www.materials.leeds.ac.uk/lemas

New MSc in Nanoscale Science and Technology
Visit http://www.ee.leeds.ac.uk/nanomsc/
______________________________

Bedtime reading..........
Series: Microscopy Handbooks - Electron Energy Loss Spectroscopy by Rik
Brydson, BIOS
Published: September 2001 £29.99 ISBN: 1859961347
Paperback; 152 pages 39 line drawings and 10 b/w half tones

**************************
Join email discussion list for all aspects of
Electron Microscopy and Analytical Spectroscopy (LEMAS)

visit website
http://www.jiscmail.ac.uk/lists/lemas.html
and follow instructions
**************************

=====================================
EELS and X ray database : http://www.cemes.fr/~eelsdb/
=====================================


--


From daemon Mon May 27 12:29:40 2002



From: cwuethri-at-caregroup.harvard.edu
Date: Mon, 27 May 2002 13:23:00 -0400
Subject: Re: Polyphenols

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Greg,

I'm afraid it's impossible to answer your question. The number of of
polyphenol is so high and can be found in so many different forms in very
different cells compartment (walls, vacuoles) associated with proteins,
sugars ... that there no single, best stain which will give you the right
answer...

In my PhD, I tried to quantify the proteins, the tannins, amyloplasts in the
bark and the xylem (last increments) of 100 adult european beeches. I used
image analysis and ... fluorescence microscopy (without staining, only with
autofluorescence).

As preliminary tests I tried some light microscopy staining which could help
you:

First you can try some old tests such as the vanillin test, the nitrous test
(Reeve, 1951; Jensen, 1962)...

If you fix your samples in Glutaraldehyde / paraformaldehyde, you will see
condensed tannins (yellow) in the cells vacuoles. If you try to stain these
fixed sections with toluidine blue (pH 4.4), these vacuoles will stain in
blue-green and the proteins in blue (the difference is not easy to
see)...Look at FEDER et O'BRIEN 1968 or O'BRIEN et al. 1964.

Some polyphenols will be black if you stain them with DMCA
(di-methyl-amino-cinnamaldehyde, also used in fluorescence microscopy).

If you simply want to localise the heartwood on a core you can perhaps
simply try to stain with lugol (there are only few amyloplasts or starch in
the heartwood).

Some people on International Tree Ring Forum (ITRDBFOR-at-listserv.arizona.edu)
will certainly give you better advices than me. Look on the net to see how
to register...

Some of the references below could perhaps help you (I do no more work on
plants and I have no more access on these articles: I don't remember the
content of these articles).

Hope it helps !!!


Chris Wuethrich
Beth Israel Deaconess Medical Center
330 Brookline AVE
Boston, MA 02215
cwuethri-at-caregroup.harvard.edu



FEUCHT W, SCHMID PPS, CHRIST E. 1986. Distribution of Flavonols in
Meristematic and Mature Tissues of Prunus avium shoots. J. Plant Physiol.
125:1-8.

FEUCHT W, TREUTTER D, CHRIST E. 1997. Role of flavanols in yellowing beech
trees of the Black Forest. Tree Physiol. 17:335-340.

FRANCESCHI VR, KREKLING T, BERRYMAN AA, CHRISTIANSEN E. 1998. Specialized
phloem parenchyma cells in norway spruce (Pinaceae) bark are an important
site of defense reactions. Am. J. Bot. 85(5):601-615.

GETACHEW G, MAKKAR HP, BECKER K. 1998. Localisation and quantification of
tannins in multipurpose tree leaves using a histochemical approach coupled
with image analysis (http://ecsoc2.hcc.ru.poster/DP_TOP6/dp278/dp278.htm).

MANSFIELD JL, CURTIS PS, ZAK DR, PREGITZER KS. 1999. Genotypic variation for
condensed tannin production in trembling aspen (Populus tremuloides,
Salicaceae) under elevated CO2 and in high- and low-fertility soil. Am. J.
Bot. 86(8):1154-1159.

MUZIKA RM, PREGITZER KS. 1992. Effect of nitrogen fertilization on leaf
phenolic production of grand fir seedlings. Trees 6:241-244.
NAGARAJAN GR, SESHADRI TR. 1964. Flavonoid components of the heartwood of
Prunus domestica Linn. Phytochem. 3:477-484.

EL MODAFAR C, CLERIVET A, MACHEIX JJ. 1996. Flavan accumulation in stems of
Platanus x acerifolia seedlings inoculated with Ceratocystis fimbriata f.sp.
platani, the canker stain disease agent. Can. J. Bot. 74:1982-1987.

GROSSONI P, BUSSOTTI F, TANI C, GRAVANO E, SANTARELLI S, BOTTACI A. 1998.
Morpho-anatomical alterations in leaves of Fagus sylvatica L. and Quercus
ilex L. in different environmental stress conditions. Chemosphere
36(4-5):919-924.






From daemon Mon May 27 20:32:07 2002



From: Beauregard :      beaurega-at-westol.com
Date: Mon, 27 May 2002 21:24:46 -0400
Subject: In situ generation of Osmium Tetroxide for the Physical

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

About a year ago I asked if anyone had done any work on the in situ
generation of osmium tetroxide. I got a few replies but nobody had a
procedure.

I stained a lot of polymers with various chemicals in the past 12 years. I
did not like to store RuO4 or OsO4 ampoules in my lab. I had an RuO4
ampoule break open in my refrigerator once. I lab-packed the remaining
ones and switched to in situ generation of RuO4 as a safer alternative.

So, I recently investigated the reactions of osmium chloride and dioxide
with various oxidizers for use on physical science samples. In my
experiments, I used about 1-3 milligrams per reaction test in a Petri dish.
Five reaction routes worked.

Discussion:
An indicator to gauge the progress of the reaction was made by dissolving
Budene® 1207 (Goodyear Tire and Rubber Company) rubber in cyclohexane
solvent. This almost colorless gum stock rubber consisted of a butadiene
rubber containing plenty of double bonds to react. This very thick
solution (almost a gel) was smeared onto a glass slide and allowed to
evaporate. This clear fairly thin film indicator system turned
progressively darker as the staining reaction proceeded and more vapors of
OsO4 were generated. It ultimately turned the indicator black on overnight
exposures.

It was observed that osmium dioxide reacted slower than osmium chloride and
both were much slower than RuCL3.xH2O. I wanted the osmium dioxide to work
and it did. However, it was very slow and required overnight exposures at
the 1-2 mg level. Don't use hydrogen peroxide with osmium dioxide powder.
It created a vigorous decompositional foaming of the hydrogen peroxide with
little generated OsO4, if any.

Osmium chloride reacted with sodium periodate (fastest), sodium
hypochlorite and hydrogen peroxide (slowest) to stain the butadiene rubber
indicator. The use of osmium chloride and hypochlorite seemed the best
choice because both chemicals were the easiest to obtain. The periodate
was a powder one had to mix and the peroxide could cause serious burns of
the skin. On the other hand, if one spilled hypochlorite on your arm, not
much would happen. The osmium chloride was not as easy to handle as RuCl3.
Reading the OsCl3 MSDS sheet could give one heart failure. There was no
free lunch here. The use of gloves, avoidance of skin contact and the
prevention of airborne dusts were critical with osmium compounds also.

In order to help gauge the progress of the reaction, it helped to have a
couple of paper towels and lastly a poly-jean cloth under the covered
reaction dishes. This provided a nice white background to see a light
stain on the indicator slide.
If the dishes broke, then only a milliliter or two of hypochlorite with a
few milligrams of osmium tetroxide would soak into the cloth and paper
towels in your hood. Cleanup would not require a HAZMAT team.

This relatively safer reaction avoided storing large amounts of Os in
opened ampoules or jars containing unused osmium tetroxide. It also
avoided managing a larger amount of waste osmium.

Disclaimer: My employer and I are not responsible for your use or safety
in handling these highly toxic and hazardous materials. Only you can
manage your risks. You must use gloves, use a fume hood, read the MSDS
sheets, avoid dusts, avoid skin, lung and eye exposures, etc. With a TWA
exposure level 2000 times lower than phosgene gas, you should get the
message that these are not casual chemicals to use without training. Talk
to someone that has previously used these materials and is a safe worker.

A more detailed procedure for generating and handling RuO4 and OsO4 was
written but apparently can't be published without tons of clearances.

Paul Beauregard
Senior Research Associate Microscopist




From daemon Tue May 28 03:12:13 2002



From: =?iso-8859-1?Q?Jes=FAs_Lamas?= :      bfsuso-at-usc.es
Date: Tue, 28 May 2002 10:04:41 +0200
Subject: Stain for glucans

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


LM Need help on staining glucans in cell walls. Do you know any "specific"
stain for glucans?.

Thanks,

Jesús Lamas



From daemon Tue May 28 06:51:09 2002



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Tue, 28 May 2002 17:13:52 +0530
Subject: [TEM] [MAT] optimum ion mill voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

I need to learn more about ion milling for TEM sample preparation. What is
considered to be the optimum milling condition with respect to voltage,
specimen current and temperature for materials which minimises beam damage
and maximises milling rate? Is it just by trial and error or are there
specific rules to consider for different classes of materials to determine
the optimum milling conditions?


----
R Divakar





From daemon Tue May 28 07:49:37 2002



From: Emmanuelle Roux :      eroux-at-caprion.com
Date: Tue, 28 May 2002 08:41:55 -0400
Subject: Re:Osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,
Like Ann, I think that the problem is your buffer or your bottle. Osmium reacts strongly with lipids and turns black right away. So to avoid that, we used extraclean bottles, always wear gloves before touching anything related to the osmium solution (you have lipids on your hands, plus osmium is highly toxic) and rinse really well with acetone the outside of the vials that contain osmium. Hope this helps.
Emmanuelle


Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620



From daemon Tue May 28 09:35:25 2002



From: cwuethri-at-caregroup.harvard.edu
Date: Tue, 28 May 2002 10:26:36 -0400
Subject: Re: Stain for pectins (glucans)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jesus,

Try 0.01-0.2% Ruthenium red in an acid solution (for example acetic acid or
Sörensen buffer) and rince with the same acidic solution or buffer. In
acidic solution ruthenium red will stain "specifically pectins. If I
remember well non methylated pectins. I guess there is another stain which
will stain more specifically the methylated pectins (try perhaps to look at
articles by Anne-Marie Catesson et al.).

As Ruthenium red is expensive, you can try to do the same with Astra Blue.
As I have seen (on 5µm beech sections embedded in JB4 resin), it will do the
same as Ruthenium red, but in bright blue...

Important notice: The fixation of the staining is reversible. In a basic
solution, the stain of the walls will migrate in the vacuoles (more acid)...
Be careful when you mont your slides that the medium is not too basic...

Hope it helps

Chris Wuethrich
Beth Israel Deaconess medical Center
330 brookline AVE
Boston, MA 02215
cwuethri-at-caregroup.harvard.edu


From daemon Tue May 28 10:11:57 2002



From: gary.m.brown-at-exxonmobil.com
Date: Tue, 28 May 2002 10:05:26 -0500
Subject: Re: In situ generation of Osmium Tetroxide for the Physical Sciences.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Paul,

Nice work. I, too, have worked extensively with in-situ production of RuO4
but never with OsO4. This will be very useful.

I applaud the sharing of your work on the list server.

Best regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



Beauregard
{beaurega-at-westo To: microscopy-at-sparc5.microscopy.com
l.com} cc: peoshel-at-facstaff.wisc.edu
Subject: In situ generation of Osmium
Tetroxide for the Physical Sciences.
05/27/02 08:24
PM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

About a year ago I asked if anyone had done any work on the in situ
generation of osmium tetroxide. I got a few replies but nobody had a
procedure.

I stained a lot of polymers with various chemicals in the past 12 years. I
did not like to store RuO4 or OsO4 ampoules in my lab. I had an RuO4
ampoule break open in my refrigerator once. I lab-packed the remaining
ones and switched to in situ generation of RuO4 as a safer alternative.

So, I recently investigated the reactions of osmium chloride and dioxide
with various oxidizers for use on physical science samples. In my
experiments, I used about 1-3 milligrams per reaction test in a Petri dish.
Five reaction routes worked.

Discussion:
An indicator to gauge the progress of the reaction was made by dissolving
Budene® 1207 (Goodyear Tire and Rubber Company) rubber in cyclohexane
solvent. This almost colorless gum stock rubber consisted of a butadiene
rubber containing plenty of double bonds to react. This very thick
solution (almost a gel) was smeared onto a glass slide and allowed to
evaporate. This clear fairly thin film indicator system turned
progressively darker as the staining reaction proceeded and more vapors of
OsO4 were generated. It ultimately turned the indicator black on overnight
exposures.

It was observed that osmium dioxide reacted slower than osmium chloride and
both were much slower than RuCL3.xH2O. I wanted the osmium dioxide to work
and it did. However, it was very slow and required overnight exposures at
the 1-2 mg level. Don't use hydrogen peroxide with osmium dioxide powder.
It created a vigorous decompositional foaming of the hydrogen peroxide with
little generated OsO4, if any.

Osmium chloride reacted with sodium periodate (fastest), sodium
hypochlorite and hydrogen peroxide (slowest) to stain the butadiene rubber
indicator. The use of osmium chloride and hypochlorite seemed the best
choice because both chemicals were the easiest to obtain. The periodate
was a powder one had to mix and the peroxide could cause serious burns of
the skin. On the other hand, if one spilled hypochlorite on your arm, not
much would happen. The osmium chloride was not as easy to handle as RuCl3.
Reading the OsCl3 MSDS sheet could give one heart failure. There was no
free lunch here. The use of gloves, avoidance of skin contact and the
prevention of airborne dusts were critical with osmium compounds also.

In order to help gauge the progress of the reaction, it helped to have a
couple of paper towels and lastly a poly-jean cloth under the covered
reaction dishes. This provided a nice white background to see a light
stain on the indicator slide.
If the dishes broke, then only a milliliter or two of hypochlorite with a
few milligrams of osmium tetroxide would soak into the cloth and paper
towels in your hood. Cleanup would not require a HAZMAT team.

This relatively safer reaction avoided storing large amounts of Os in
opened ampoules or jars containing unused osmium tetroxide. It also
avoided managing a larger amount of waste osmium.

Disclaimer: My employer and I are not responsible for your use or safety
in handling these highly toxic and hazardous materials. Only you can
manage your risks. You must use gloves, use a fume hood, read the MSDS
sheets, avoid dusts, avoid skin, lung and eye exposures, etc. With a TWA
exposure level 2000 times lower than phosgene gas, you should get the
message that these are not casual chemicals to use without training. Talk
to someone that has previously used these materials and is a safe worker.

A more detailed procedure for generating and handling RuO4 and OsO4 was
written but apparently can't be published without tons of clearances.

Paul Beauregard
Senior Research Associate Microscopist









From daemon Tue May 28 10:16:05 2002



From: Alexander Black :      alexander.black-at-nuigalway.ie
Date: Tue, 28 May 2002 16:10:32 +0100
Subject: Microscopical Society of Ireland - symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Information on the Microscopical Society of Ireland Symposium for
2002 and abstract submission forms etc. is now up at
www.nuigalway.ie/msi

Sincerely,
Alexander Black



From daemon Tue May 28 10:25:44 2002



From: Gordon Nord :      gnord-at-mindspring.com
Date: Tue, 28 May 2002 11:19:13 -0400
Subject: Re: [TEM] [MAT] optimum ion mill voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For ceramics and minerals the damage rate varies considerably.
I use 6 keV for most minerals except oxides.
Oxides require 0.5 keV for 24 hours and LN2 cooling stage to remove
surface damage.
Silicates on the other hand can thin at 6 keV and suffer minimum damage.

Temperatures can get fairly high so if you are interested in
transformation microstructures be aware.
A LN2 stage is very useful.

As a rule of thumb you get the best results it you start with a thin
material.
10 micrometers thick is a good start, if possible.

Your results will vary, speaking from would you believe, 30 years of
experience.

Gordon Nord
Environmental Sciences Laboratory
Brooklyn College

On Tuesday, May 28, 2002, at 07:43 AM, Divakar R wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers:
}
} I need to learn more about ion milling for TEM sample preparation. What
} is
} considered to be the optimum milling condition with respect to voltage,
} specimen current and temperature for materials which minimises beam
} damage
} and maximises milling rate? Is it just by trial and error or are there
} specific rules to consider for different classes of materials to
} determine
} the optimum milling conditions?
}
}
} ----
} R Divakar
}
}
}
}



From daemon Tue May 28 10:43:45 2002



From: Mayandi Sivaguru :      sivagurum-at-missouri.edu
Date: Tue, 28 May 2002 10:36:27 -0500
Subject: Re: Stain for glucans

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jesús, Do you want to stain beta 1,3 or 1,4 glucans. And then do you prefer
antibodies or fluoroscent stains. There are stains such as Calcofluor,
Aniline blue and Sirofluor stains either one of these or both glucans.
Monoclonal antibodies are also available from
http://www.biosupplies.com.au/product.html, an Australian company. Do you
want to use with plants, yeast or in human cells to study activation of
immune response involving macrophages. If you could be more specific with
detail may help answering better.

Shiv

Dr. Mayandi Sivaguru, Ph.D.,
Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2, Tucker Hall
University of Missouri
Columbia, MO 65211-7400
USA

Voice: 1-573-882-4895
Fax: 1-573-882-0123
Home: 1-573-445-6037

www.biotech.missouri.edu/mcc/


Following are the sample products from Biosupplies might be relevant to you.
Enzymes:
(1-3;1-4)-beta-D-Glucan Hydrolase from Bacillus subtilis (EC 3.2.1.73).
Specifically hydrolyses beta-D-glucans containing both (1-3)- and
(1-4)-beta-D-glucosidic linkages in linear sequences. Does not hydrolyse
(1-4)-beta-D-glucans or (1-3)-beta-D-glucans [Anderson & Stone (1975) FEBS
Letters 52: 202-207].

Substrates:
Pachyman [(1-3)-beta-D-Glucan] ex Poria cocus
Useful as a positive control in fluoroescence microscopy studies on callose
using the aniline blue fluorochrome or (1-3)-beta-D-glucan specific
monoclonal antibody.
Carboxymethyl-pachyman [(1-3)-beta-D-Glucan] (D.S. 0.31)
This water-soluble O-carboxymethyl ether of pachyman is a useful substrate
for assay of (1-3)-beta-D-glucan endo-hydrolases either reductometrically
or viscometrically.
(1-3;1-4)-beta-Glucan from Barley
A water-soluble polysaccharide from walls of barley endosperm cells. Useful
as a substrate for (1-3;1-4)-beta-D-glucan hydrolase and as a control in
immunohistochemical studies with the (1-3;1-4)-beta-D-glucan monoclonal
antibody [Meikle et al (1994) The Plant Journal 5: 1-9].
Arabino-(1-4)-beta-D-Xylan from wheat flour
A water-soluble polysaccharide from walls of wheat endosperm cells. Useful
as a substrate for (1-4)-beta-D-xylan hydrolase.

Histochemical Reagents:
Aniline Blue Fluorochrome for callose
An analytical probe for (1-3)-beta-D-glucans (callose). Highly specific for
(1-3)-beta-glucans [Evans et al. (1984) Carbohydrate Polymers 4: 215-230;
Stone et al. (1984) Protoplasma 122: 191-195]. May be used for the
quantitative determination of callose [Kauss (1989) Plant Physiol. 81:
171-176].
Yariv Reagents for arabinogalactan-proteins
Specific probes for the detection of arabinogalactan-proteins in tissue
sections [Anderson et al. (1977) Aust J. Plant Physiol. 4: 143-158]. May be
used to detect and quantify arabinogalactan-proteins in tissue extracts
[Van Holst & Clarke (1985) Anal Biochem. 148: 446-450] and to detect
arabinogalactan-proteins in crossed-electrophoretic separations [Van Holst
& Clarke (1986) Plant Physiol. 80: 786-798]

Immunohistochemical Reagents:
Monoclonal Antibodies (Murine)
to (1-3)-beta-D-Glucan
No cross-reactivity with (1-4)-beta-D-glucans or (1-3;1-4)-beta-D-glucans
[Meikle et al. (1991) Planta 188: 1-8].
to (1-3;1-4)-beta-D-Glucan
No cross-reactivity with (1-3)-beta-D-glucans [Meikle et al. (1994) The
Plant Journal 5: 1-9].
Each of these antibodies can be used with second stage, gold- or
fluorochrome-labelled rabbit, anti-mouse antibody for immunohistochemical
studies.




From daemon Tue May 28 11:06:00 2002



From: Raynald Gauvin :      raynald.gauvin-at-mcgill.ca
Date: Tue, 28 May 2002 11:58:38 -0400
Subject: Workshop on Charging Materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

On August 2 and 3, 2002, there will be a workshop on Characterization of
Non-Conductive or Charging Materials with Microbeam Analysis. This workshop
will be held at McGill University which is located in Montreal, Quebec,
Canada. It is still possible to register to this workshop, the deadline
have been postpone up to the end of July. For information about lodging and
update of the scientific program, please go to

http://www.minmet.mcgill.ca/charging

Also note that on Saturday, august 3 after 3h00 pm, it will be possible to
attendees to take a special bus to go to Quebec city. The cost will be 25$
canadian.

I will be please to meet you at McGill next august.

Raynald GAUVIN




From daemon Tue May 28 11:46:11 2002



From: Jian-Guo Zheng :      j-zheng3-at-northwestern.edu
Date: Tue, 28 May 2002 11:36:39 -0500
Subject: qx2000 EDX pulse procesor and computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Friends,

I have an old link EDX detector made in England. Its series no is
23088-1380-01. ATT NO: 32CC6080D, 0120024. Model: 6547. Det. Area: 30
mmxmm. Winder: ATW2. Resolution: 136 at 5.9 KeV.

Currently, the X-ray count rate is quite low (less than 200 counts/sec)
even when beam was strong and sample was thick. This problem occurred
when our Link pulse processor (QX 2000) was replaced by a new pulse
processor produced by a new company. I thought the new pulse processor
did not get along with the Link detector, which caused the problem.
After the investigation of several months, an engineer from the company
told me that my detector suffered from hard X-rays or backscattered
electrons which hit the detector. The hard X-rays could frequently reset
the EDX system and cause the low count rate.
Therefore, the low rate may be related to both the detector and the
processor. First I would like to know your opinion about possible
reasons about the hard X-ray and back-scattering electrons. Second, I
need a Link pulse processor (XQ2000) (mine was dead) to see the
performance of the detector. Please let me know if you have one and no
longer use it. I want to identify the reasons one by one. Any other
suggestions are welcome.

I am looking forward to your response.

Jian-Guo Zheng
EPIC
Materials Science & Engineering
Northwestern University
2225N Campus Drive, 1156 Cook Hall
Evanston, IL 60208-3108, USA
Phone: (847) 491-7807, Fax: (847) 491-7820
E-mail: j-zheng3-at-northwestern.edu





From daemon Tue May 28 12:29:53 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Tue, 28 May 2002 18:25:02 +0100
Subject: Re: In situ generation of Osmium Tetroxide for the Physical Sciences.

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I'll second that
I also suggest this gets written up in Microscopy Today with Paul's
name on it
Chris
Paul,

Nice work. I, too, have worked extensively with in-situ production of
RuO4
but never with OsO4. This will be very useful.

I applaud the sharing of your work on the list server.

Best regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



Beauregard
{beaurega-at-westo To:
microscopy-at-sparc5.microscopy.com
l.com} cc:
peoshel-at-facstaff.wisc.edu
Subject: In situ
generation of Osmium
Tetroxide for the Physical
Sciences.
05/27/02 08:24
PM





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The Microscopy ListServer -- Sponsor: The Microscopy Society of
America


Hi,

About a year ago I asked if anyone had done any work on the in situ
generation of osmium tetroxide. I got a few replies but nobody had a
procedure.

I stained a lot of polymers with various chemicals in the past 12
years. I
did not like to store RuO4 or OsO4 ampoules in my lab. I had an RuO4
ampoule break open in my refrigerator once. I lab-packed the
remaining
ones and switched to in situ generation of RuO4 as a safer
alternative.

So, I recently investigated the reactions of osmium chloride and
dioxide
with various oxidizers for use on physical science samples. In my
experiments, I used about 1-3 milligrams per reaction test in a Petri
dish.
Five reaction routes worked.

Discussion:
An indicator to gauge the progress of the reaction was made by
dissolving
Budene® 1207 (Goodyear Tire and Rubber Company) rubber in cyclohexane
solvent. This almost colorless gum stock rubber consisted of a
butadiene
rubber containing plenty of double bonds to react. This very thick
solution (almost a gel) was smeared onto a glass slide and allowed to
evaporate. This clear fairly thin film indicator system turned
progressively darker as the staining reaction proceeded and more
vapors of
OsO4 were generated. It ultimately turned the indicator black on
overnight
exposures.

It was observed that osmium dioxide reacted slower than osmium
chloride and
both were much slower than RuCL3.xH2O. I wanted the osmium dioxide to
work
and it did. However, it was very slow and required overnight
exposures at
the 1-2 mg level. Don't use hydrogen peroxide with osmium dioxide
powder.
It created a vigorous decompositional foaming of the hydrogen peroxide
with
little generated OsO4, if any.

Osmium chloride reacted with sodium periodate (fastest), sodium
hypochlorite and hydrogen peroxide (slowest) to stain the butadiene
rubber
indicator. The use of osmium chloride and hypochlorite seemed the
best
choice because both chemicals were the easiest to obtain. The
periodate
was a powder one had to mix and the peroxide could cause serious burns
of
the skin. On the other hand, if one spilled hypochlorite on your arm,
not
much would happen. The osmium chloride was not as easy to handle as
RuCl3.
Reading the OsCl3 MSDS sheet could give one heart failure. There was
no
free lunch here. The use of gloves, avoidance of skin contact and the
prevention of airborne dusts were critical with osmium compounds also.

In order to help gauge the progress of the reaction, it helped to have
a
couple of paper towels and lastly a poly-jean cloth under the covered
reaction dishes. This provided a nice white background to see a light
stain on the indicator slide.
If the dishes broke, then only a milliliter or two of hypochlorite
with a
few milligrams of osmium tetroxide would soak into the cloth and paper
towels in your hood. Cleanup would not require a HAZMAT team.

This relatively safer reaction avoided storing large amounts of Os in
opened ampoules or jars containing unused osmium tetroxide. It also
avoided managing a larger amount of waste osmium.

Disclaimer: My employer and I are not responsible for your use or
safety
in handling these highly toxic and hazardous materials. Only you can
manage your risks. You must use gloves, use a fume hood, read the
MSDS
sheets, avoid dusts, avoid skin, lung and eye exposures, etc. With a
TWA
exposure level 2000 times lower than phosgene gas, you should get the
message that these are not casual chemicals to use without training.
Talk
to someone that has previously used these materials and is a safe
worker.

A more detailed procedure for generating and handling RuO4 and OsO4
was
written but apparently can't be published without tons of clearances.

Paul Beauregard
Senior Research Associate Microscopist











From daemon Tue May 28 20:45:27 2002



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From daemon Tue May 28 22:41:21 2002



From: R Divakar :      divakar-at-igcar.ernet.in
Date: Wed, 29 May 2002 09:09:57 +0530
Subject: [TEM] [MAT] optimum ion mill voltage

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The samples I am trying to prepare for HRTEM are nanocrystalline palladium.

Best Wishes,
Divakar

-----Original Message-----
} From: Peter Tomic [SMTP:PTomic-at-anadigics.com]
Sent: Wednesday, May 29, 2002 9:08 AM
To: Divakar R


Dear Listers:

I need to learn more about ion milling for TEM sample preparation. What is
considered to be the optimum milling condition with respect to voltage,
specimen current and temperature for materials which minimises beam damage
and maximises milling rate? Is it just by trial and error or are there
specific rules to consider for different classes of materials to determine
the optimum milling conditions?


----
R Divakar







From daemon Wed May 29 03:36:42 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 29 May 2002 09:30:31 +0100 (GMT Daylight Time)
Subject: Re: qx2000 EDX pulse procesor and computer

Contents Retrieved from Microscopy Listserver Archives
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Hi Jian-Guo,

A few thoughts for you.

An important question is - why has this problem started
since you changed the processor, surely the problem woud
have existed before if it is a microscope or a detector
shielding problem?

You do not say what instument your detector is on. From the
crystal size I would guess that it is on a TEM is this
correct? If not then you can discount hard X-rays and
backscattered electrons. Can you operate at a much lower
voltage and improve the problem, x-rays can only be as
energetic as the accelerating voltage?

I assume that the objective apertures and all other
possible sources of BSEs and x-rays in the secimen region
are out. Do you have a 'hard x-ray aperture' above the
specimen or in the condensor strip that should be used? Are
you in a mag range and not in low mag when the objective
lens is weak and may allow more electrons to hit the
detector?

If you get high energy x-rays or electrons into the
detector you will get low counts but very high dead time as
the detector is spending most of it's time recognising and
rejecting unsuitable events (not in the 0-20kV range).

However, I would expect the operation without the
microscope beam on to be fine, that is the zero strobe or
noise peak should be a few hundred cps, good resolution (50
to 60eV in your case) and no other signals.

If this changes when the beam is on but the specimen is not
in it may be high energy x-rays. Retracting the detector
should change (but may not eliminate) this.

If you get a high dead time with low counts when the
specimen is in can you reduce the beam current and reduce
the counts? Does changing the processing time change the
deadtime significantly? If counts increase as the beam
current drops then you have too many counts and should keep
reducing the beam current to get a good operating condition.

It is possible to recognise electrons or high energy x-rays
if you can look at the processor ramp with an oscilloscope.
In an EDX detector the crystal operates as a reverse bias
diode, when is has no x-ray input there will be a voltage
ramp that is reset at a certain voltage. This will be seen
as a sawtooth wave form with a period of milliseconds
(maybe several 100s of ms). When an x-ray is detected the
ramp has a jump equivalent to the energy of the x-ray. If
an electron enters the ramp may see the negative charge of
the electron at the leading edge of the jump. If either an
electron or a high energy x-ray enters the jump height will
be very large. This causes the ramp to reset very often and
gives a high deadtime. Your engineer, who is telling you
that it is a high energy or x-ray problem, should be able
to demonstrate this to you and determine which it is.

If it really is caused by electrons or high energy x-rays
this is best discussed with you microscope manufacturer. I
still wonder why it was not a problem before, maybe a look
in the column is needed, collimator damaged, lost specimen
blocking x-ray beam etc. and probe is very strong as you
don't have a 'real' x-ray sgnal from your specimen.

Good luck,
Ron

On Tue, 28 May 2002 11:36:39 -0500 Jian-Guo Zheng
{j-zheng3-at-northwestern.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, Friends,
}
} I have an old link EDX detector made in England. Its series no is
} 23088-1380-01. ATT NO: 32CC6080D, 0120024. Model: 6547. Det. Area: 30
} mmxmm. Winder: ATW2. Resolution: 136 at 5.9 KeV.
}
} Currently, the X-ray count rate is quite low (less than 200 counts/sec)
} even when beam was strong and sample was thick. This problem occurred
} when our Link pulse processor (QX 2000) was replaced by a new pulse
} processor produced by a new company. I thought the new pulse processor
} did not get along with the Link detector, which caused the problem.
} After the investigation of several months, an engineer from the company
} told me that my detector suffered from hard X-rays or backscattered
} electrons which hit the detector. The hard X-rays could frequently reset
} the EDX system and cause the low count rate.
} Therefore, the low rate may be related to both the detector and the
} processor. First I would like to know your opinion about possible
} reasons about the hard X-ray and back-scattering electrons. Second, I
} need a Link pulse processor (XQ2000) (mine was dead) to see the
} performance of the detector. Please let me know if you have one and no
} longer use it. I want to identify the reasons one by one. Any other
} suggestions are welcome.
}
} I am looking forward to your response.
}
} Jian-Guo Zheng
} EPIC
} Materials Science & Engineering
} Northwestern University
} 2225N Campus Drive, 1156 Cook Hall
} Evanston, IL 60208-3108, USA
} Phone: (847) 491-7807, Fax: (847) 491-7820
} E-mail: j-zheng3-at-northwestern.edu
}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed May 29 15:44:07 2002



From: Susan Carbyn :      CarbynS-at-EM.AGR.CA
Date: Wed, 29 May 2002 14:59:27 -0400
Subject: Maintenance question

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Hi!

We have a Hummer VII sputter coater, and I know the oil has not been changed in many years. I wanted to change the oil, but have no instructions on how to do this - and the person who was responsible for this machine is now gone. The drain and fill points are obvious, but are there any things that I need to be aware of before doing this? How do I go about ballasting the oil? Please bear with the questions - as in the past - I was only a user of the equipment - not someone who has experience in maintaining it.

Thanks for any information!

Susan



Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, N.S. B4N 1J5
Canada

Phone (902) 679-5535
Fax (902) 679-2311

E-Mail: carbyns-at-em.agr.ca



From daemon Wed May 29 16:01:44 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 29 May 2002 15:49:04 -0500
Subject: toluidine again

Contents Retrieved from Microscopy Listserver Archives
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Regarding my Toluidine Blue stain solution, which should be staining animal
tissue varying shades of blue-purple (which I want), but is now staining
bright blue instead (which I don't want):

1% Toluidine Blue O
1% Sodium Tetraborate Dodecahydrate
30% Ethanol
in DDI

(1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)

I've received several suggestions to change the pH--some to acidic, some to
basic. Which direction should it be changed and what chemical do I use to
change it?

Thank you again,

Jaclynn M. Lett, Research Technician
Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu
314-977-0257


From daemon Wed May 29 16:39:05 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 29 May 2002 17:33:03 -0400
Subject: Re: toluidine again

Contents Retrieved from Microscopy Listserver Archives
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Depends on the type of sections. You only need the tetraborate (which makes
the stain very alkaline) if you are trying to stain through epoxy (Epon,
Araldite, Spurr) sections. If you want metachromasia (varing shades of
blue/purple/red), you must have an acidic solution. If you are staining wax or
frozen sections, eliminate the tetraborate, eliminate the alcohol, and cut the
stain concentration to 0.1% or less in distilled water (I use 0.05%), rinse in
water and dehydrate in acetone, not alcohol, to preserve the metachromasia. You
can use various buffers to get the pH lower than 4 for even more selective
staining.
If you are staining epoxy sections, keep the tetraborate but eliminate the
alcohol. Alcohol destroys metachromasia, at least in TB stained tissue.

Jaclynn Lett wrote:

} Regarding my Toluidine Blue stain solution, which should be staining animal
} tissue varying shades of blue-purple (which I want), but is now staining
} bright blue instead (which I don't want):
}
} 1% Toluidine Blue O
} 1% Sodium Tetraborate Dodecahydrate
} 30% Ethanol
} in DDI
}
} (1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)
}
} I've received several suggestions to change the pH--some to acidic, some to
} basic. Which direction should it be changed and what chemical do I use to
} change it?
}
} Thank you again,
}
} Jaclynn M. Lett, Research Technician
} Electron Microscopy Core Facility
} Fay and Carl Simons Center for Biology of Hearing and Deafness
} Central Institute for the Deaf
} 4560 Clayton Ave.
} St. Louis, MO 63110
}
} jlett-at-cid.wustl.edu
} 314-977-0257

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed May 29 17:35:13 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 29 May 2002 18:12:30 -0400
Subject: [TEM] [MAT] optimum ion mill voltage

Contents Retrieved from Microscopy Listserver Archives
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There are a number of things that are important with respect to ion milling. Since you are doing HREM, you want to minimize surface damage and since you want to work with nanocrystalline material, you want to minimize beam heating to prevent grain growth or phase changes. As you increase the milling angle, you increase the rate of etching up to a maximum at about 20 degrees, however, several significant things will happen, 1) the sample heating goes up, 2) the surface damage by ion implantation goes up, and 3) topographic features are enhanced and can grow. Going to a lower energy at these high angles can improve things, but not by much. The best thing to do is to go to low angles and low voltage. Both of these will minimize the effects above and the low angle will "smooth" features out and actually polish the sample, but at the expense of time. That is why the low angle ion mills need and have higher current ion guns than the older ion mills.

To speed things up a little, you can go to a higher angles to mill for awhile, drop the angle to the lowest that you can go (and still hit the desired area), and then finish at a lower energy. On the newer mills, I would not go above 10 degrees to start. I typically will start at 6-8 degrees and drop to about 4 degrees. This is the lowest that my dimple + sample holder will allow me. Since I use a single sided dimple, I can have the gun on the flat side of the sample at a little lower angle. Fortunately, my samples are glass and I can see the samples fluoresce when the beam hits them. My samples are also thinned as thin as they permit and still be able to handle them so that the dimple depth is as shallow as possible. This also decreases the lowest possible angle. I really don't like to go above 5 kV, but when I am in a bit more of a hurry, I will start at 6 kV. I finish at about 2.5 kV at 4 degrees. One thing that you should remember, that most of the guns on commercially
available ion mills lose their focus at lower voltages, i.e. the beam broadens and the current density decreases. The one exception is the Gentle Mill from South Bay Technology that uses Arpa Barna's low energy guns. The main thing to realize for your HREM application is that the damage layer on top and bottom of your sample will be minimized the lower the voltage and the lower that angle. There is a considerable difference in the available mills on the market with respect to the milling rates at different voltages and lowest possible angles.

I would recommend that you take a piece of soda lime glass (ordinary window glass) and prepare a sample to the same thickness and dimple it to the same dimensions that you will use on your sample. Then lower the ion mill angle to the lowest that you can and still see the center of the dimple lit up. With a dimple in the sample, you will actually see a shadow come across the center of the dimple when the shoulder of the dimple is shadowing the beam. This will give you an indication for the lowest angle that you can get to. A glass sample will also serve as an alignment sample for the ion guns. Nothing slows down ion milling better than guns that are out of alignment. This sample is best if you just use about a 100 um thick sample that is parallel sided.

I also suggest that you get a hold of the third installment of the MRS books on TEM sample preparation and look at Arpa Barna's paper. In the words of Ron Anderson, "He taught the world how to ion mill."

I hope this helps.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)


-----Original Message-----
} From: Divakar R [mailto:divakar-at-igcar.ernet.in]
Sent: Tuesday, May 28, 2002 7:44 AM
To: microscopy-at-sparc5.microscopy.com


Dear Listers:

I need to learn more about ion milling for TEM sample preparation. What is
considered to be the optimum milling condition with respect to voltage,
specimen current and temperature for materials which minimises beam damage
and maximises milling rate? Is it just by trial and error or are there
specific rules to consider for different classes of materials to determine
the optimum milling conditions?


----
R Divakar



From daemon Wed May 29 18:57:59 2002



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Thu, 30 May 2002 09:53:53 +1000
Subject: Re: toluidine again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day All,
For epoxy sections you require high pH Tol. Blue stain and heat ~60c.
After staining, rinse the sections and dry the slide before
coversliping.
Do not use alcohol or excessive heat to dry the slide, both will destroy
the metachromacy. You require some water in the section for metachromacy
to remain viable.
Best to mount the coverslip in a water based mounting media, this
prevents the bleaching of the tol.blue stain that so often happens with
xylene based styrene mountants.
Regards
JVN

Geoff McAuliffe wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Depends on the type of sections. You only need the tetraborate (which makes
} the stain very alkaline) if you are trying to stain through epoxy (Epon,
} Araldite, Spurr) sections. If you want metachromasia (varing shades of
} blue/purple/red), you must have an acidic solution. If you are staining wax or
} frozen sections, eliminate the tetraborate, eliminate the alcohol, and cut the
} stain concentration to 0.1% or less in distilled water (I use 0.05%), rinse in
} water and dehydrate in acetone, not alcohol, to preserve the metachromasia. You
} can use various buffers to get the pH lower than 4 for even more selective
} staining.
} If you are staining epoxy sections, keep the tetraborate but eliminate the
} alcohol. Alcohol destroys metachromasia, at least in TB stained tissue.
}
} Jaclynn Lett wrote:
}
} } Regarding my Toluidine Blue stain solution, which should be staining animal
} } tissue varying shades of blue-purple (which I want), but is now staining
} } bright blue instead (which I don't want):
} }
} } 1% Toluidine Blue O
} } 1% Sodium Tetraborate Dodecahydrate
} } 30% Ethanol
} } in DDI
} }
} } (1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)
} }
} } I've received several suggestions to change the pH--some to acidic, some to
} } basic. Which direction should it be changed and what chemical do I use to
} } change it?
} }
} } Thank you again,
} }
} } Jaclynn M. Lett, Research Technician
} } Electron Microscopy Core Facility
} } Fay and Carl Simons Center for Biology of Hearing and Deafness
} } Central Institute for the Deaf
} } 4560 Clayton Ave.
} } St. Louis, MO 63110
} }
} } jlett-at-cid.wustl.edu
} } 314-977-0257
}
} Geoff
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************

--
John V Nailon
Executive Officer and Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Wed May 29 21:14:22 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 29 May 2002 15:49:04 -0500
Subject: toluidine again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hummer coaters usually have the rotary pumps installed in the console.

If you can remove the oil using a tube of some type: great.

If not, there are four nuts holding the pump down that can be easily
accessed from the bottom.

Once the nuts & the pumping hose on the top of the pump are removed the pump
can be easily removed for servicing.

Regards,

Earl

----- Original Message -----
} From: "Susan Carbyn" {CarbynS-at-EM.AGR.CA}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 29, 2002 11:59 AM


Regarding my Toluidine Blue stain solution, which should be staining animal
tissue varying shades of blue-purple (which I want), but is now staining
bright blue instead (which I don't want):

1% Toluidine Blue O
1% Sodium Tetraborate Dodecahydrate
30% Ethanol
in DDI

(1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)

I've received several suggestions to change the pH--some to acidic, some to
basic. Which direction should it be changed and what chemical do I use to
change it?

Thank you again,

Jaclynn M. Lett, Research Technician
Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu
314-977-0257



From daemon Wed May 29 21:53:46 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 29 May 2002 15:49:04 -0500
Subject: toluidine again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Regarding my Toluidine Blue stain solution, which should be staining animal
tissue varying shades of blue-purple (which I want), but is now staining
bright blue instead (which I don't want):

1% Toluidine Blue O
1% Sodium Tetraborate Dodecahydrate
30% Ethanol
in DDI

(1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)

I've received several suggestions to change the pH--some to acidic, some to
basic. Which direction should it be changed and what chemical do I use to
change it?

Thank you again,

Jaclynn M. Lett, Research Technician
Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu
314-977-0257



From daemon Thu May 30 01:44:11 2002



From: Petr Schauer :      Petr-at-isibrno.cz
Date: Thu, 30 May 2002 08:35:18 +0200
Subject: list of producers & vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear microscopists,

The page with microscopy Producers & Vendors at my Petr's Microscopy
Resources (http://www.petr.isibrno.cz/microscopy/production.php) has
been modified and extended.

New links (not only in Production & Sale category) will be
appreciated. If your URL (laboratory, company, congress, journal,
..,) isn't in the Petr's Microscopy Resources or if you have found an
interesting URL, please fill in the simple form at the
http://www.petr.isibrno.cz/microscopy/PMRform.php. Your submission
will be reviewed and included within one day.

Regards,

Petr Schauer


+--------------------------------------------------------------------+
| Dr. Petr Schauer tel.: (+420 5) 41514313 |
| Head of Electron Microscopy Laboratory fax : (+420 5) 41514404 |
| INSTITUTE OF SCIENTIFIC INSTRUMENTS (+420 5) 41514402 |
| ACADEMY OF SCIENCES OF THE CZECH REPUBLIC e-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno petr-at-dp.fce.vutbr.cz |
| Czech Republic www: http://www.petr.isibrno.cz/ |
+--------------------------------------------------------------------+



From daemon Thu May 30 05:09:34 2002



From: Tony Bruton :      Bruton-at-nu.ac.za
Date: Thu, 30 May 2002 11:50:13 +0200
Subject: Looking for a replacement Board - Oxford eXLII system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Greetings All

If anyone has a spare or redundant PC1916 Computer Board for our ailing
Oxford eXLII system we would really like to hear about it.

I am told that there is one available at Oxford in the UK but the cost
is beyond us.

Our serial number for the board is 1490 - i1J
ass no. W - 0140276
PC1916 1128 - 184 - V3

Numbers for the system : Model no. 110 - 012
Serial no. 16112
ass no. W - 0140530

Thanks in anticipation.



Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website: www.nu.ac.za/microscopy.asp
Email: bruton-at-nu.ac.za
Postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa


From daemon Thu May 30 07:10:07 2002



From: volfova-at-pobox.sk (by way of Ask-A-Microscopist)
Date: Thu, 30 May 2002 06:59:06 -0500
Subject: Ask-A-Microscopist: staining of polystyrene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (volfova-at-pobox.sk) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 30, 2002 at 04:10:19
---------------------------------------------------------------------------

Email: volfova-at-pobox.sk
Name: Volfova Petra

Organization: Faculty of Chemical and Food Technology,Slovak
University of Technology,Department Plastics and Rubber

Education: Graduate College

Location: Bratislava, Slovak republic

Question: I have got some problems with the staining of
polystyrene/poly(butyl acrylate) core/shell particles with OsO4 and
uranyl acetate UA combination. There are not contrast between core
and shell. When I used only either UA or OsO4, I obtained the same
particles without contrast. Only when I functionalized either core or
shell of particles for example carboxyl groups, there were contrast
between core and shell, if I used the UA only. I predict reactions
between -COOH and UA. But where I do the mistake? I thik the time of
staining is very important factor. What do you think?
Thank you very much.


---------------------------------------------------------------------------


From daemon Thu May 30 08:04:12 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Thu, 30 May 2002 13:57:28 +0100
Subject: X-ray diffraction - JCPDS data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,
sorry to go a bit off topic and stray into X-ray land, but I have an
offer (and a question) which I hope someone can help me with.

First, I have a microfiche set of 1987 JCPDS crystal data files (with
microfiche reader) which is free for anyone to have if they want it. Too
expensive to throw away. If you want it you will have to collect/pay for
someone to collect it for you.

Second, I am looking for crystallographic data on TiW and TiW(N), which I do
not have even in my more recent CD-ROM JCPDS data files. I would like
details of lines seen up to 85 degrees 2 theta with Cu Kalpha radiation. I
suspect that there is no data on this, but would prefer to be proved
wrong...

Many thanks,

Richard



_______________________________
Richard Beanland
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com




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From daemon Thu May 30 08:20:20 2002



From: lillianft-at-aol.com (by way of Ask-A-Microscopist)
Date: Thu, 30 May 2002 08:08:47 -0500
Subject: Ask-A-Microscopist: SEM- simple procedures for tissue preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lillianft-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 30, 2002 at 08:07:21
---------------------------------------------------------------------------

Email: lillianft-at-aol.com
Name: lillian Domenico

Organization: College of St. Elizabeth

Education: Undergraduate College

Location: City, State, Country Morristown, NJ

Question:
I will be teaching an introductory course on SEM in the Fall. I am
in the process of looking for simple procedures for tissue
preparations. Do you have any lab manuals you could recommend?
Thanks
Lillian Domenico

---------------------------------------------------------------------------


From daemon Thu May 30 10:54:45 2002



From: K.N. Bozhilov :      bozhilov-at-citrus.ucr.edu
Date: Thu, 30 May 2002 08:46:17 -0700
Subject: EM400 STEM available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A Philips EM400 STEM is available for sale in Riverside, CA.
It is a 24 years old instrument with W filament, ±60° goniometer tilt, with
EDAX EDS system PV9100 and Si(Li) detector. The scope is not operational due
to minor vacuum problem. The V7 valve needs to be fixed or replaced.

We are open to discuss any offers.


Krassimir Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel 909 787 2998
Fax 909 787 4324




From daemon Thu May 30 11:46:44 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 30 May 2002 09:38:43 -0700
Subject: Re: Maintenance question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Susan,
We had an old Hummer for years, I think it was a Hummer I, and I recall that
the oil drain could have a small hose attached to drain the oil. Just drain
it all out and add new to the right place on the site glass. To "ballast"
the oil just means to run a bit of air through it to drive off all the water
vapour or other solvent vapours that might be in the fresh oil. Don't pump
on a wide open inlet, just a little air leak for about ten minutes to clean
the oil. I recall that my Hummer had an air leak valve that you could
control to bleed a little air into the chamber.
Hope this helps.
At 02:59 PM 05/29/2002 -0400, you wrote:
}
} Hi!
}
} We have a Hummer VII sputter coater, and I know the oil has not been
changed in many years. I wanted to change the oil, but have no instructions
on how to do this - and the person who was responsible for this machine is
now gone. The drain and fill points are obvious, but are there any things
that I need to be aware of before doing this? How do I go about ballasting
the oil? Please bear with the questions - as in the past - I was only a
user of the equipment - not someone who has experience in maintaining it.
}
} Thanks for any information!
}
} Susan
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu May 30 11:59:47 2002



From: gary.m.brown-at-exxonmobil.com
Date: Thu, 30 May 2002 11:52:19 -0500
Subject: Re: Ask-A-Microscopist: staining of polystyrene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Volfova,

I recommend that you stain with ruthenium tetroxide. If my memory serves me
correctly, polystyrene is stained heavily with RuO4 whereas
poly(butylacrylate) is not. By comparison, OsO4 stains neither molecule
particularly well.

My recommendation is that you cut cryosections of the material and stain
these for several minutes in the vapors of an RuO4 solution. Allow the
stained sample to degas in the hood for a bit prior to handling and
microscopy. Alternatively, one might consider include staining the block
face for 30 minutes to several hours (just a guess on the staining
duration) followed by cryogenic sectioning for TEM. Only experimentation
will allow you to determine the best method for you materials. I also
recommend that you develop staining protocols on majority/minority blends
of known volume fraction. This ensures that you know beyond a doubt the
reactivity of the stain to each blend component. Once this is known, you
can attack the sample of concern with confidence in your ability to
properly interpret the results.

OsO4 and RuO4 stain by different mechanisms. Therefore, take care not to
overstain the material with RuO4. RuO4 may rapidly stain many materials in
a matter of minutes to hours whereas the same materials appear relatively
impervious to overstaining with OsO4 regardless of staining duration.

I wish you the best,

Gary M. Brown
phone: (281) 834-2387




volfova-at-pobox.s
k (by way of To: Microscopy-at-sparc5.microscopy.com
Ask-A-Microscop cc:
ist) Subject: Ask-A-Microscopist: staining of
polystyrene

05/30/02 06:59
AM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (volfova-at-pobox.sk) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 30, 2002 at 04:10:19
---------------------------------------------------------------------------

Email: volfova-at-pobox.sk
Name: Volfova Petra

Organization: Faculty of Chemical and Food Technology,Slovak
University of Technology,Department Plastics and Rubber

Education: Graduate College

Location: Bratislava, Slovak republic

Question: I have got some problems with the staining of
polystyrene/poly(butyl acrylate) core/shell particles with OsO4 and
uranyl acetate UA combination. There are not contrast between core
and shell. When I used only either UA or OsO4, I obtained the same
particles without contrast. Only when I functionalized either core or
shell of particles for example carboxyl groups, there were contrast
between core and shell, if I used the UA only. I predict reactions
between -COOH and UA. But where I do the mistake? I thik the time of
staining is very important factor. What do you think?
Thank you very much.


---------------------------------------------------------------------------







From daemon Thu May 30 12:13:04 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 30 May 2002 12:56:24 -0400
Subject: Re: Toluidine Blue O - Metachromasia and Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning All,

For those among you who are wise, you should probably immediately
jump or GOTO END!

This is difficult to put into words without chancing a gross error
or causing some offence, but here goes anyway.

When we "fix" a specimen taken from a biological source, we NEVER
quite know what we are doing. The entire process is 'outcome-based'
(Ugh!!!). How does it look? Does the fixative 'coagulate' or 'cross-link'
the myriad of chemical entities within the sample? What is the effect of
specimen size? What about temperature? How long to kill? How long to
achieve minimum preservation? Preservation of what? What will constitute
comparable samples that permit statistical comparison of specimens of mouse
and elephant liver? We preserve or fix or kill or maintain color or get rid
of it. Behind every act there lies a reason, and in consequence of every
act there is produced an uncountable number of variables which only
REPRESENT what existED in the original, living, organism. Everyone says
that s/he already knows this!

Metachromasia is part physical chemistry, part biochemistry (and by
reason of those two, for many, little more than pure magic!). Metachromasia
has been extensively used, extensively studied and extensively reported
upon. Several items of information are clear [This may be an
exaggeration!].

1. The polychrome effect noted in the use of several related dyes
of the thiazine (quinone-imine/methylene blue) family is based on the
polymerization of dye molecules and the distances between adjacent acidic
groups on the substrate.

2. A loss of the polychrome effect could be attributed to*:
a. decrease in the concentration of some tissue/substrate
component
b. decarboxylation [- or - esterification with alcohol?]
c. degradation of the substrate to diffusible constituents.

[* see Pearse, A.G.E.(1985), Histochemistry, Theoretical and
Applied(4th Ed.), Vol II, Churchill Livingstone, NY,NY, pp701-710. ISBN:
0-443-02997-0.

3. In order for the metachromatic color. for (Toluidine Blue O,
that is purple [red + blue]) to be observed, the appropriately spaced acid
moieties must be deprotonated, i.e. negative in charge. Otherwise, the
orthochromatic color is observed.

In other words, in order for there to be metachromasia of a
particular component of the tissue, that component must have appropriately
spaced negatively charged groups.

4. At pH 7, most 'acid' moieties in biological systems are
deprotonated, and thus, negatively charged. The isoelectric points of most
macromolecules are in the vicinity of pH 5 [This IS an exaggeration!] Where
pH can determine dye binding, pH can be used to partition objects in the
specimen space along a pH gradient [also in Pearse, same pages, but see
Methylene Blue Extinction (MBE) methods in many compendia]. [MBE used to
distinguish among histologic 'acid' mucosubstances (GAG's, etc.).]

5. A buffer may stabilize substances, react with them, or promote
changes in them (i.e. oxidation).

6. A bottle of dye, used for 5 years to produce a result, may, at
some moment, STOP behaving as it should. Pearse mentions that one of the
reasons that metachromasia was such a confused subject prior to the 50-60's,
was due to the fact that so many studies failed to use pure dyes.

RULE: If a dye fails to function as it should. Try a different batch or
make up a fresh batch, or purchase a new supply. I have found that a simple
0.1% solution of the dye gives rise to regular, reproducible metachromasia
which survives dehydration in absolute ethanol, but never in 95% ethanol.
That having been said, when I have performed the Azure B, pH 4.0 for
ethanol-acetic acid(3:1)(Clark) fixed nucleic acids (Flax and Himes, 1952),
I follow their protocol for dehydration and use tertiary butanol.
Preparations I made in the mid-60's still show metachromasia, albeit with
some overall loss of color in my personally prepared Damar-xylene mountant.

The questions about the failure of any regularly used dyeing
procedure amount to a scientific challenge that are best addressed by the
one who is having the problem. Since the variables are many, the sources of
failure are also many. One has to learn how to perform a component analysis
in order to efficiently address such a problem. Since much of what one does
in dyeing/staining is by prescribed protocol, it should be clear that if
anything has changed, it is the operator who is in the best position to DO
the troubleshooting. When I ran the Flax and Himes procedure, I always
retained the blocks of previously sectioned material. Each sectioned block
was dipped in paraffin to cover the exposed tissue and stored carefully
away. I was especially careful of those specimens, prepared for any
particular purpose, in the event I ever required a 'known' tissue source of
a good result. Even so, I was aware that the stored specimens would not be
the same in two years as those I sectioned yesterday. I learned this when I
addressed the issue of saving tissues labeled with tritiated thymidine.
Why, I asked parenthetically, should I be able to determine that loss of
radioactivity by disintegration was not going to be augmented by loss due to
progressive destruction of DNA, if I had no specific knowledge of how much
DNA/nucleus/section was present in the starting material? So, I learned
that I would not be able to have compete faith, even in the best of my
archived specimens, 5 or 10 years in the future.

NOTE: Acridine orange was one of the first fluorescent dyes used by
virologists in the late 50's/early 60's. One was able to distinguish
between single stranded RNA and double-stranded DNA until someone noted the,
then recent, discovery of the RNA viruses. "Nuts!" Even now, there is an
extensive literature on the use of AO fluorescence for double-dyeing the
nucleic acids in cell nuclei.

An absolute obligation of old windbags is a SUMMARY: If there is a
single point in all of this, it is this. In the application of
metachromasia there are considerations of mass action, pH, purity of the
dye, and treatments preceding and following the application of the dye. If
each adds an order of magnitude to the number of variables involved, there
are 4-5 such ordinal magnifications through the process. If the physical
and chemical bases of histologic methods are understood at less than optimal
levels, then troubleshooting will be a problem that has little hope for
success. On the other hand, even one who has only the recipe to which s/he
can refer can be taught component analysis of that recipe.

Mom used to say, "If you don't know what kind of flour to put in
your bread, try any flour and the bread will let you know if you were right
or wrong. If you were wrong, and really want bread, then you will have to
try another flour, and another, until the bread tells you that you are
finally right. Just don't change any other part of the recipe while you are
testing the flour." Ah! If only more of us had learned to bake when we
were young.

END: Respectfully submitted,

Fred Monson


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Thu May 30 12:14:38 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 30 May 2002 13:08:44 -0400
Subject: RE: Maintenance question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Afternoon Susan,
I think you can read the information you want at the following site.
It's simple, straight forward, and I couldn't find anything wrong with the
procedure to replace the oil.

URL: http://safescience.ucsc.edu/Equipinst/vacpump.html

You might also want to know that I was recently given a nice pump that
hadn't been used for a while. Along with it came a case of oil and a case
of what was called Pump Flush. I used 4 quarts of the flush before the
cleaning was done. You might expect the same from small amounts of oil.

If you are interested in the flushing fluid, contact Savant Industries at
1-800-634-8886, and ask for information on "Savant Vacuum Pump flushing
Fluid", catalog No., SFF-1. I think that it works REALLY well.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Susan Carbyn
} Sent: Wednesday, May 29, 2002 2:59 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Maintenance question
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi!
}
} We have a Hummer VII sputter coater, and I know the oil has not been
} changed in many years. I wanted to change the oil, but have no
} instructions on how to do this - and the person who was responsible for
} this machine is now gone. The drain and fill points are obvious, but are
} there any things that I need to be aware of before doing this? How do I
} go about ballasting the oil? Please bear with the questions - as in the
} past - I was only a user of the equipment - not someone who has experience
} in maintaining it.
}
} Thanks for any information!
}
} Susan
}
}
}
} Susan Carbyn
} Electron Microscopy Technician
} Atlantic Food and Horticulture Research Centre
} Agriculture and Agri-Food Canada
} 32 Main Street, Kentville, N.S. B4N 1J5
} Canada
}
} Phone (902) 679-5535
} Fax (902) 679-2311
}
} E-Mail: carbyns-at-em.agr.ca
}
}
}


From daemon Thu May 30 12:31:47 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 30 May 2002 13:30:16 -0400
Subject: Optical Microscopy of Fine Wire in Microelectronic Application

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;

Has anyone found an optical microscopy solution to measuring microelectronic
bond wire loop height and length in one instrument? Our problem is that we
want to measure the length of a wire [.001" diameter] that has been formed
into a loop connecting two points in a ckt. This is generally not a
critical measurement but at our frequency range small variations in this
formation can mean large electrical parasitic effects.

Regards,

Peter Tomic
Anadigics, Inc.


From daemon Thu May 30 13:48:42 2002



From: Robert Fitton :      fittonro-at-luther.edu
Date: Thu, 30 May 2002 12:42:54 -0600
Subject: ETEC SEM available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

Our near and dear to my heart ETEC autoscan is going to be replaced this
summer by a more up to date SEM. The ETEC is in working order and comes
with a complete set of spare parts.

Please contact me directly if interested. The scope is located in NE Iowa.

Cheers,

Robert

Robert Fitton
Teaching Associate/Director of Laboratories
Luther College
Department of Biology
700 College Drive
Decorah, IA 52101

Voice 563-387-1559
FAX 563-387-1080




From daemon Thu May 30 14:47:04 2002



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Thu, 30 May 2002 12:37:04 -0700
Subject: Eutectics Neuron Tracing System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
We will be de-commissioning a Neuron Tracing System, by Eutectics
Electronics, available to anyone who can make use of it, or who might
need it for parts. It is fully functional.

Free to a good home for the cost of shipping.

HP 2-pen plotter, lots of pens
Gateway 80486 computer, with monitor
Mark 4 stage with joystick and controller, extra old-style joystick and
stage motors
4 K vector graphics processor
Vector graphics display
Focus drive
NTS software version 5
sorry, we are keeping the venerable Zeiss WL microscope.



Regards,
Glen

--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************


From daemon Thu May 30 18:21:43 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 30 May 2002 16:10:06 -0700
Subject: Re: Toluidine Blue O - Metachromasia and Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fred

I don't understand your point about Acridine Orange (AO)? How discovery of
the RNA-viruses affects the staining and our knowledge about this
particular staining agent? As I remember, AO staining is based on polar
interactions, mostly with "nucleic acids". The 'color' of fluorescence
depends from the energy transfer efficiency between donor (AO) and acceptor
(which may be, may be not nucleic acid). I expect, the 'color' from AO
stained double strands RNA would be similar to DNA. Another point here, as
far as I know, RNA viruses in most cases contain "double-stranded RNA
segments", so it's a mixture single/double-stranded RNA. I could not
predict how it will fluorescence with AO. Nevertheless, such viruses are
very small and I don't believe you could even see their fluorescence with
AO on the autofluorescence background of the typical cell. The bottom line
here: the discovery of the new "RNA-viruses" is not affected our knowledge
of basic properties of AO and AO would work (I believe) at the same manner
with this 'double-stranded RNA' as with other stuff. I don't see big
difference between single/double-stranded-RNA and DNA. It's well known,
that most RNAs tends to form secondary structure (call it double-stranded
RNA fragments) under the physiological conditions. Moreover, my personal
experience shows that it's extremely difficult to make RNA completely
'single-stranded': even at the presence of 8MUrea+formamide,

Sergey



At 09:56 AM 5/30/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu May 30 20:04:02 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 30 May 2002 17:58:40 -0700
Subject: FEI/Philips XL-30 high vac uptime

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could anyone respond with typical uptime for
the Philips XL-30 high vac system? This is the
FESEM version. At issue are multiple users,
stupid things that can happen, cost of the stupid
things, frequency thereof, etc.

would appreciate all feedback. Private off-line is
preferred.

tnx,
gary g.



From daemon Thu May 30 21:06:38 2002



From: Ask-A-Microscopist :      zaluzec-at-ultra5.microscopy.com
Date: Thu, 30 May 2002 20:56:12 -0500
Subject: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Status:
}
} Hi,
}
} A colleague asked an interesting question the other day: were there
} any legal requirements for microscope images?
}
} The only issues which I have seen are the following:
} 1. The standard format set by MSA is TIFF.
} 2. Ethically, an image can be processed for improved publication but
} not to any extent which corrupts data. Better microscopy is
} strongly preferred over processing.
}
} Do any of you know of any other legal ramifications?
}
} Thanks in advance for any input.
}
} Best regards,
} Barbara Foster
} Microscopy/Microscopy Education
} 125 Paridon Street, Suite 102
} Springfield, MA 01118
} PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
}


From daemon Fri May 31 05:59:20 2002



From: =?iso-8859-1?q?Jeremy=20Sanderson?= :      jb_sanderson-at-yahoo.com
Date: Fri, 31 May 2002 11:49:21 +0100 (BST)
Subject: Information on Moire Fringes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
Can anyone please point me in the direction of easily
understandable information on the nature of Moire
fringes. I have a good article from Scientific
American dated 1963(!), but have almost no other
information.
I have tried Google and a search, but need to sort out
the good from the bad/misleading. Any leads would be
much appreciated.
Thanks, Jeremy
jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com


From daemon Fri May 31 06:27:01 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 31 May 2002 12:19:55 +0000
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Processing is not ethical if it corrupts data?? Hmm--- strictly, any
processing *at all* corrupts the primary image data.
Most people would accept a bit of sharpening or noise reduction or
contrast enhancement as part of the normal image preparation
process - except of course where the absolute values of pixels are
important, as in ratio imaging for example. Even conventional silver
photographers do this routinely, by choosing a development
protocol and printing paper grade to optimise image contrast, or by
dodging or holding back during printing, or by spotting out dust and
scratches. Even some sharpening or blurring was possible using
conventional non-digital technique, by choosing appropriate
condensors in the enlarger, etc. The names of the digital
equivalents of these manipulations derive from their applications in
silver imaging - viz. the dodging tool and unsharp mask sharpening
in Photoshop, so there is nothing new about a bit of image
processing in the interests of optimal data presentation.

Furthermore, what is the status of image processing techniques for
noise reduction such as image averaging / integration, fourier
transformation / summation / filtration / reconstruction. What about
ratio and subtraction images - are these to be regarded as corrupt
data too? Most people regard these as techniques for data
acquisition and enhancement rather than data corruption, and thus
the end-process of the image processing IS the original primary
image data.

So another definition of corruption of data is required. In my view,
the issue should not be whether the image has been processed or
not. If there is a role for a legal definition at all, the question should
be "at what point does processing cross the line (if there is such a
thing) into image manipulation intended to misrepresent or
mislead?". My guess is that it will always be very hard to provide a
single answer to that, as to so many other legal questions. Why
else do we have juries?

Chris

} } Hi,
} }
} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is
} } strongly preferred over processing.
} }
} } Do any of you know of any other legal ramifications?
} }
} } Thanks in advance for any input.
} }
} } Best regards,
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Fri May 31 07:12:35 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Fri, 31 May 2002 07:02:40 -0500
Subject: Re: Maintenance question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My .02....

Typically, the ultimate vacuum achieved will be reduced while the ballast
valve is open. Vacuum should improve on closure.

I back-fill my coaters (as well as the SEM) with the off-gas from my liquid
nitrogen cryo. My vacuum pump oil must be treated as both radioactive and a
hazmat, therefore changes are expensive and infrequent. Given this reality,
I "ballast" the pumps often using the dry N2. I have not done a study to
quantify the improvement by using dry N2 in "flushing" the oil of
contaminants, but it should be more effective than air (especially in humid
environments).
_W_

Woody White
McDermott Technology Inc.
McD: http://www.rdd.mcdermott.com/
Mine: http://woody.white.home.att.net


-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Thursday, May 30, 2002 12:39 PM
To: Susan Carbyn
Cc: Microscopy-at-sparc5.microscopy.com


Dear Susan,
We had an old Hummer for years, I think it was a Hummer I, and I recall that
the oil drain could have a small hose attached to drain the oil. Just drain
it all out and add new to the right place on the site glass. To "ballast"
the oil just means to run a bit of air through it to drive off all the water
vapour or other solvent vapours that might be in the fresh oil. Don't pump
on a wide open inlet, just a little air leak for about ten minutes to clean
the oil. I recall that my Hummer had an air leak valve that you could
control to bleed a little air into the chamber.
Hope this helps.
At 02:59 PM 05/29/2002 -0400, you wrote:
}
} Hi!
}
} We have a Hummer VII sputter coater, and I know the oil has not been
changed in many years. I wanted to change the oil, but have no instructions
on how to do this - and the person who was responsible for this machine is
now gone. The drain and fill points are obvious, but are there any things
that I need to be aware of before doing this? How do I go about ballasting
the oil? Please bear with the questions - as in the past - I was only a
user of the equipment - not someone who has experience in maintaining it.
}
} Thanks for any information!
}
} Susan
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri May 31 07:41:15 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 31 May 2002 08:39:52 -0400
Subject: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barbara;

With respect to legal requirements of images, and by that I assume you mean
ownership, I would suggest reviewing copyright laws that apply. We often ask
our clients/customers to sign NDA's [Non-Disclosure Agreements] since many
of our analytical reports contain proprietary information and images.

I would also suggest checking with some forensic people since images,
electronic or otherwise, in criminal investigations is critical. There was
someone at an MSA conference in Monterey, CA from FBI back in ~ 1993 that
gave a good talk on this subject.

Hope this helps some.

Peter Tomic
Anadigics, Inc.


-----Original Message-----
} From: Ask-A-Microscopist [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Thursday, May 30, 2002 9:56 PM
To: Microscopy-at-sparc5.microscopy.com


} Status:
}
} Hi,
}
} A colleague asked an interesting question the other day: were there
} any legal requirements for microscope images?
}
} The only issues which I have seen are the following:
} 1. The standard format set by MSA is TIFF.
} 2. Ethically, an image can be processed for improved publication but
} not to any extent which corrupts data. Better microscopy is
} strongly preferred over processing.
}
} Do any of you know of any other legal ramifications?
}
} Thanks in advance for any input.
}
} Best regards,
} Barbara Foster
} Microscopy/Microscopy Education
} 125 Paridon Street, Suite 102
} Springfield, MA 01118
} PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
}


From daemon Fri May 31 07:51:33 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 31 May 2002 08:44:41 -0400
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barbara,
A number of years ago I had a customer in a state crime lab and he told
me that any digital images had to be captured on a WORM drive (Write
Once, Read Many), now CD-R. This was considered the rough equivalent of
a photographic negative in court.

Also, there were several threads over the past couple of years about
enhancing (altering) data. I believe the main thrust was that you had
to have the original data and also be able to describe (preferably with
equations) what was done to alter the data to its final state. The
threads go into much more detail.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Status:
} }
} } Hi,
} }
} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is strongly
} } preferred over processing.
} }
} } Do any of you know of any other legal ramifications?
} }
} } Thanks in advance for any input.
} }
} } Best regards,
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
}
}





From daemon Fri May 31 08:17:58 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Fri, 31 May 2002 09:10:21 -0400
Subject: Optical Microscopy of Fine Wire in Microelectronic Application

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter,
We have an optical based micrometer attached to our Zeiss Axiophot and
Axiovert stage. this gives Z in 1 micron increments. The units are made by
Boeckeler Instruments who did the modification / attachment. The sensitivity
will of course depend on the quality of the stage drive.
Russ Gillmeister
Xerox
~~~~~~~~~~~~~


-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Thursday, May 30, 2002 1:30 PM
To: Microscopy Listserver (E-mail)


Folks;

Has anyone found an optical microscopy solution to measuring microelectronic
bond wire loop height and length in one instrument? Our problem is that we
want to measure the length of a wire [.001" diameter] that has been formed
into a loop connecting two points in a ckt. This is generally not a
critical measurement but at our frequency range small variations in this
formation can mean large electrical parasitic effects.

Regards,

Peter Tomic
Anadigics, Inc.


From daemon Fri May 31 09:10:46 2002



From: Carlton, Robert :      robert.carlton-at-elan.com
Date: Fri, 31 May 2002 10:05:18 -0400
Subject: Correction: PSM Meeting (Longwood Gardens, June 6)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



To all,

There is a typographical error in the meeting notice for the Philadelphia
Society of Microscopy notice for our meeting at Longwood Gardens on June
6th. The email address should read

jreffner-at-rohmhaas.com


We put too many r's in the notice. Alternatively, you can simply reply to
me at this email address.

Our apologies for the inconvenience.

Robert A. Carlton
Elan Drug Delivery, Inc.
3500 Horizon Drive
King of Prussia, PA, 19406
610-313-1360
robert.carlton-at-elan.com



********************************************************************
This communication and any files transmitted with it
may contain information that is confidential, privileged
and exempt from disclosure under applicable law.
It is intended solely for the use of the individual or entity
to which it is addressed. If you are not the intended
recipient, you are hereby notified that any use,
dissemination or copying of this communication is strictly
prohibited. If you have received this communication in
error, please notify the sender. Thank you for your co-operation.
********************************************************************



From daemon Fri May 31 09:28:47 2002



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 31 May 2002 09:19:43 -0500
Subject: Re: Information on Moire Fringes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you seen.....

Reyes-Gasga J., Tehuacanero S., and Yacaman M. Jose. 1998. Moire
Patterns in High Resolution Electron Microscopy Images of MoS2.
Microsc. Res. Tech. 40:2-9, 1998.
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================

Jeremy Sanderson wrote:


------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.

Dear All,
Can anyone please point me in the direction of easily
understandable information on the nature of Moire
fringes. I have a good article from Scientific
American dated 1963(!), but have almost no other
information.
I have tried Google and a search, but need to sort out
the good from the bad/misleading. Any leads would be
much appreciated.
Thanks, Jeremy
jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com





From daemon Fri May 31 11:17:34 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 31 May 2002 12:16:34 -0400
Subject: Re: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This thread seems to reflect a common phenomenon that accompanies
technological progress: in effect, that when a more objective and
quantifiable means of accomplishing something becomes possible, it is held to
a higher standard than the technology or prior art that preceded it. For
example, DNA evidence has been subjected to a level of quantified review that
fingerprinting has generally not. Newly synthesized drugs are routinely
evaluated prior to legalization in ways that botanicals have seldom been
scrutinized.

Since digitized images are readily subject to quantifiable changes that, as
Ken says, may be described numerically, it doesn't strike us as odd to suggest
that changes made to them should be scrutinized in that way. And yet every
step in conventional photography is susceptible to substantial variations, the
sum of which is probably beyond quantifying. (Imagine! One is steeping a
sheet of film in chemical after chemical in an attempt to produce a stain that
resembles something seen by the eye!)

What of that hallowed original negative? I wonder how often photographic
evidence has been subjected to a rigorous review of how each step in its
storage and development affected its response (log e) characteristics,
contrast, density range, etc. And what about color?!. How long did time and
storage temperature act on the silver halide grain ripening? Were water
quality, time, temperature and agitation quantified at each step of
development, rinsing and fixing? What was the age of each of the baths and
the number of prior images processed at the time of this development? Was the
image tested for residual fixer or halide? How has this stained piece of
plastic and gelatin been stored since? How much highlight fading has it
undergone as a result of sulfur-containing gases and peroxides acting on the
image silver? Now, if dealing with a print instead of a negative, simply
repeat each of these variables and add them to the calculation. Next, if
there was intentional manipulation of the development in order to increase the
capabilities of the film, quantify that.

Leaving aside the enormous variations in color stability among different film
types, what of the inherent ability of dyes and pigments to render color? As
every critical photographer knows, for every film / print combination there
are certain colors that cannot be rendered accurately. Similarly, the means
by which manufacturers bias the intrinsic sensitivities of their CCD chips to
render an approximation of what the eye sees are not perfect (nor accessible
to the user, as they often form the basis for proprietary advancements in
their equipment.)

Along with prompting a review of what may be deemed acceptable within the
potentials of new imaging technology these advances should, perhaps, provoke
some thought about what the real level of accountability has been in regard to
the older technology.

John Twilley
Conservaton Scientist

Ken Converse wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Barbara,
} A number of years ago I had a customer in a state crime lab and he told
} me that any digital images had to be captured on a WORM drive (Write
} Once, Read Many), now CD-R. This was considered the rough equivalent of
} a photographic negative in court.
}
} Also, there were several threads over the past couple of years about
} enhancing (altering) data. I believe the main thrust was that you had
} to have the original data and also be able to describe (preferably with
} equations) what was done to alter the data to its final state. The
} threads go into much more detail.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} Delta, PA
}
} Ask-A-Microscopist wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } } Status:
} } }
} } } Hi,
} } }
} } } A colleague asked an interesting question the other day: were there
} } } any legal requirements for microscope images?
} } }
} } } The only issues which I have seen are the following:
} } } 1. The standard format set by MSA is TIFF.
} } } 2. Ethically, an image can be processed for improved publication but
} } } not to any extent which corrupts data. Better microscopy is strongly
} } } preferred over processing.
} } }
} } } Do any of you know of any other legal ramifications?
} } }
} } } Thanks in advance for any input.
} } }
} } } Best regards,
} } } Barbara Foster
} } } Microscopy/Microscopy Education
} } } 125 Paridon Street, Suite 102
} } } Springfield, MA 01118
} } } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} } }
} }
} }





From daemon Fri May 31 14:00:40 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 31 May 2002 08:54:11 -1000 (HST)
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, All-

The Education Committee of the Microscopy Society of America has recently
formed a new subcommittee on the ethics of digital image manipulation,
which intends to promote discussion of this issue. At M&M2002 in Quebec
City I'll be bringing it up during the Problem Solving with the Experts
session, which is scheduled for Tuesday at 8:30 am.

Currently, as far as I know, there are no legal or ethical standards for
digital image presentation except in clinical and forensic fields where
images may be used in legal cases. If anyone knows of any other standards,
please let me know.

I have some opinions and ideas, and I had planned to bring it up in this
list soon so I could get an idea how all of you felt, and then summarize
and invite more discussion at the meeting. I welcome any and all input!

I'll have a lot more to say as soon as I have time to write, but this is
as good a time as any to let the debate begin!


} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is
} } strongly preferred over processing.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Fri May 31 14:00:42 2002



From: max.sidorov-at-amd.com
Date: Fri, 31 May 2002 11:51:45 -0700
Subject: Information on Moire Fringes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeremy,
I just did a google on this. Second item at the top of the search list:
http://don.sci.mu.edu/classes/L1980210.htm

{- it's about gratings but for crystals it's the same principle. Moire fringes are interference patterns produced by two overlapping lattices or gratings.

Hope this helps,

Max
__________________________________
Max Sidorov
Materials Technology Development
Advanced Micro Devices

max.sidorov-at-amd.com



-----Original Message-----
} From: Jeremy Sanderson [mailto:jb_sanderson-at-yahoo.com]
Sent: Friday, May 31, 2002 3:49 AM
To: Microscopy-at-sparc5.microscopy.com


Dear All,
Can anyone please point me in the direction of easily
understandable information on the nature of Moire
fringes. I have a good article from Scientific
American dated 1963(!), but have almost no other
information.
I have tried Google and a search, but need to sort out
the good from the bad/misleading. Any leads would be
much appreciated.
Thanks, Jeremy
jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com




From daemon Fri May 31 14:12:22 2002



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Fri, 31 May 2002 12:05:00 -0700
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The real test is that the individual that is introducing the photo must be able to testify that the image "truly and accurately depicts what it is purported to represent." That is the question that we are asked when a photo is being introduced in court. If you can testify in court that it does so it will probably be introduced. Even an image taken with a wide angle or telephoto lens does not meet this criteria if being introduced to show size relative to distance, but would for some other uses.

A lot of people have developed rules they believe the courts would want, like the one stated here, but I am unaware of any standard that has been set down by the courts themselves (appellate or above in published opinions) other than the "accurately depicts" test. If your testimony were challenged on that issue it would be of great help to have an original that you could prove had not been altered in any way. Our department is going to a great deal of trouble to set up a system that provides this. But introduction of the image does not require that, at least with-in the jurisdictions I've worked in.

Jim

James L. Roberts
Supervising Forensic Scientist
Comparative Analysis Section
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 654-2308

James.Roberts-at-mail.co.ventura.ca.us


} } } "Ken Converse" {qualityimages-at-netrax.net} 05/31/02 05:44AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Barbara,
A number of years ago I had a customer in a state crime lab and he told
me that any digital images had to be captured on a WORM drive (Write
Once, Read Many), now CD-R. This was considered the rough equivalent of
a photographic negative in court.

Also, there were several threads over the past couple of years about
enhancing (altering) data. I believe the main thrust was that you had
to have the original data and also be able to describe (preferably with
equations) what was done to alter the data to its final state. The
threads go into much more detail.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Status:
} }
} } Hi,
} }
} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is strongly
} } preferred over processing.
} }
} } Do any of you know of any other legal ramifications?
} }
} } Thanks in advance for any input.
} }
} } Best regards,
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
}
}









From daemon Fri May 31 14:40:31 2002



From: Clarkson Donna R Contr USAMRD :      donna.clarkson-at-brooks.af.mil
Date: Fri, 31 May 2002 14:33:45 -0500
Subject: TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking to purchase a digital camera system for our TEM and
would like some feedback from those of you who already utilize such systems.
Some questions I'd like to ask are:

* What make & model camera system do you have?
* What type, make & model printer(s) do you use, (e.g. inkjet,
dye-sublimation, silver halide)?
* Do you feel you get micrograph-quality resolution and images
utilizing that system?
* Ease of use--is it user-friendly or cumbersome?
* Ease and/or quality of service--are any of the components
serviceable by you? How quickly & easily is it to get a service rep?
* How long have you had this system?
* Would you recommend this system--why or why not?
* What, if anything, might you have done differently?

I'd greatly appreciate any responses. Thank-you in advance for your
help.

Donna R. Clarkson


From daemon Fri May 31 15:44:36 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 31 May 2002 16:30:43 -0500
Subject: Manipulation of images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

James Roberts wrote:
=====================================================
The real test is that the individual that is introducing the photo must be
able to testify that the image "truly and accurately depicts what it is
purported to represent." That is the question that we are asked when a
photo is being introduced in court. If you can testify in court that it does
so it will probably be introduced. Even an image taken with a wide angle or
telephoto lens does not meet this criteria if being introduced to show size
relative to distance, but would for some other uses.

A lot of people have developed rules they believe the courts would want,
like the one stated here, but I am unaware of any standard that has been set
down by the courts themselves (appellate or above in published opinions)
other than the "accurately depicts" test. If your testimony were challenged
on that issue it would be of great help to have an original that you could
prove had not been altered in any way. Our department is going to a great
deal of trouble to set up a system that provides this. But introduction of
the image does not require that, at least with-in the jurisdictions I've
worked in.
==========================================================
One must always remember that the expert giving such testimony is rendering
professional judgements and opinions and if one or another party to a
dispute should believe or try to construe that the independent expert made a
professional error, which resulted in economic loss to them, then that
expert can be sued for professional malpractice just as a physician or any
other professional can be sued in our courts by anyone who feels they have
been harmed by the professional negligence of the expert.

This reality becomes a powerful force to make sure sure that any image
enhancement or alteration is done in ways that do not distort the outcome of
one's conclusions. Remember,just as for physicians and lawyers, one does
not really have to have made a profesisonal error, since someone needs only
to construe that an error was made, for them to be on the receiving end of a
law suit.

We all know that lawyers and physicians pay a great deal of money for
insurance to fund such losses. But people rendering opinions on microscopy
results tend to forget about the need for such insurance coverage, even
though many times they put their own personal assets at risk when they
render such testimony (without professional liablity insurance coverage).

Do laboratories get sued? You bet. Anyone doing this type of work should
have some kind of a program of loss prevention and risk analysis in order to
reduce the risk that any of their laboratory's results could be
misinterpreted or misconstrued by others.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================


From daemon Fri May 31 16:02:41 2002



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 31 May 2002 15:55:04 -0500 (CDT)
Subject: SEM specimen holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone,

We are using an Hitachi S-570 SEM for examination of rock thin sections
mounted on 1" x 3" glass microscope slides. Up until now, we have been
securing the slides to a large circular (50 mm) specimen holder with
copper and/or carbon tape. We would like a holder that would not require
the use of tape, carbon paint, etc. Does anyone know if there are any
commercially available holders (similar, for example, to that found in a
Cameca Microprobe, where a metal frame secures and grounds the slide to
the base) that would work in our microscope?

Any design ideas would also be appreciated since our machine shop will
fabricate what we need as long as nothing is commercially available.

Thanks for any help,

Heather Owen

Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall, P.O. Box 413
Milwaukee, WI 53210
USA

Phone: (414)229-6816



From daemon Fri May 31 17:29:54 2002



From: Hiromi Konishi :      hkonishi-at-asu.edu
Date: Fri, 31 May 2002 15:21:34 -0700
Subject: Cerius Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{fontfamily} {param} Times_New_Roman {/param} Cerius users: 5/31/02

{/fontfamily} {fontfamily} {param} Times {/param}

I am having troubles with image simulations along low-index
orientations using Cerius program (Cerius 2 Ver.4).


I redefined unit-cell and changed the orientation that I want to
calculate to a primary axis. I divided the new cell into several slices
and I calculated projected potential for each slice. However, the
resulting ED patterns have extra spots that dose not match to the
reciprocal lattice points of the original unit-cell. I thought the
extra spots are from high order Laue zone, but the spacing does not
match to that I expect in some cases.


What I would like to ask are:

(1) How can I interpret the calculated ED patterns, assuming that
Cerius does not go wrong?

(2) If Cerius code has troubles, at what conditions does it go wrong?

(3) Is there any other programs that allow us to calculate low-index
orientation images?


The msi files of the structures, log files, and the calculated
diffraction patterns, and copies of comments I received from a user and
the developer are available upon request.


I would appreciate any comments you might have.


Hiromi konishi hkonishi-at-asu.edu {smaller}

{/smaller} {/fontfamily}




From daemon Fri May 31 18:10:45 2002



From: gary.m.brown-at-exxonmobil.com
Date: Fri, 31 May 2002 18:03:15 -0500
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The real test is that the individual that is introducing the photo must be
able to testify that the image "truly and accurately depicts what it is
purported to represent." That is the question that we are asked when a
photo is being introduced in court. If you can testify in court that it
does so it will probably be introduced. Even an image taken with a wide
angle or telephoto lens does not meet this criteria if being introduced to
show size relative to distance, but would for some other uses.

A lot of people have developed rules they believe the courts would want,
like the one stated here, but I am unaware of any standard that has been
set down by the courts themselves (appellate or above in published
opinions) other than the "accurately depicts" test. If your testimony were
challenged on that issue it would be of great help to have an original that
you could prove had not been altered in any way. Our department is going to
a great deal of trouble to set up a system that provides this. But
introduction of the image does not require that, at least with-in the
jurisdictions I've worked in.

Jim

James L. Roberts
Supervising Forensic Scientist
Comparative Analysis Section
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 654-2308

James.Roberts-at-mail.co.ventura.ca.us


} } } "Ken Converse" {qualityimages-at-netrax.net} 05/31/02 05:44AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Barbara,
A number of years ago I had a customer in a state crime lab and he told
me that any digital images had to be captured on a WORM drive (Write
Once, Read Many), now CD-R. This was considered the rough equivalent of
a photographic negative in court.

Also, there were several threads over the past couple of years about
enhancing (altering) data. I believe the main thrust was that you had
to have the original data and also be able to describe (preferably with
equations) what was done to alter the data to its final state. The
threads go into much more detail.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Status:
} }
} } Hi,
} }
} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is strongly
} } preferred over processing.
} }
} } Do any of you know of any other legal ramifications?
} }
} } Thanks in advance for any input.
} }
} } Best regards,
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
}
}












From daemon Fri May 31 18:14:29 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 31 May 2002 18:05:15 -0500
Subject: Re: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Actually, in addition to guidelines for generation of images,
there can be issues of data ownership and legal ramifications based
on interpretation of micrographs by the microscopists. These and
other related questions will be discussed at M&M2002 during the
Technologist's Forum Roundtable. The topic for this session is:
Legal and Ethical Issues of Data Ownership".

The panel will consist of: Bertha M Knoppers, an internationally
recognized expert in ethics and the law, as well as representatives
from both academia and industry.

Hope many of you can attend because responses to questions related
to this topic are not always as obvious as one would expect.
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907


On Thursday, May 30, 2002 8:56 PM, Ask-A-Microscopist
{zaluzec-at-sparc5.microscopy.com} wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Fri May 31 18:14:47 2002



From: David Hall :      hall-at-aecom.yu.edu
Date: Fri, 31 May 2002 18:05:47 -0500
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barbara -

The National Forensic Science Technology Center maintains a site for The
Scientific Working Group for Imaging Technologies at
http://for-swg.org/it_files/swgit_guidelines.html. The site includes draft
guidelines for use of imaging technologies by criminal justice
professionals.

James Martin
Orion Analytical, LLC
www.orionanalytical.com
martin-at-orionanalytical.com


----- Original Message -----
} From: "Ask-A-Microscopist" {zaluzec-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 30, 2002 9:56 PM


There are two more significant legal aspects that might be brought up.

1. To whom does the microscope image belong as intellectual
property, and under what conditions can the image be shared between
groups, placed on a website, or published in someone's manuscript.
How does one adequately attribute the original source of the image? I
certainly have experienced occasional surprise when an old workprint
of mine has been scanned to create a new published illustration.

2. For a previously published microscope image, how much
manipulation transforms the data into a "new" image which can be
published without violating the copyright on the original publication?

Obviously we need a lawyer or two on this list. Does anyone know of
a good article or book that covers this ground?
--
David H. Hall, Ph.D.
Center for C. elegans Anatomy
Department of Neuroscience
Albert Einstein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461

www.wormatlas.org
www.aecom.yu.edu/wormem

phone 718 430-2195
fax 718 430-8821


From daemon Fri May 31 22:45:24 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Fri, 31 May 2002 23:30:58 -0400 (EDT)
Subject: Re: Manipulation of images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The most basic rule is that the controls and experimentals must be
treated the same before they are imaged and that they are imaged by the
same parameters.
Following the simple rules of doing valid controls and
taking the raw data in a uniform manner is far more critical than
the post-collection manipulation.

And the problems with the image manipulation are not that images are
altered intentionally, but that imaging amateurs (who may be the most
brilliant
geneticists or endocrinologists), who don't understand digital
representations, misuse Photoshop. A very common example is the misuse of
the Auto button in the Levels or Curves menu when rescaling from 12 bits
to 8 bits or to-byte-as-shown in any of the imaging packages. And then
the question we get is how to fix the problem, not to
compound it. "Why do the WT and KO look the same on the computer when I
swear the WT looked at least 2 times brighter through the eyepiece and the
Westerns show a 10X reduction in expression in the KO?"

People who want to cheat will always find a way to do so. The real
problem is educating to avoid simple imaging errors.


-Michael

___________________________________
WORK: http://www.aecom.yu.edu/aif/




From daemon Sat Jun 1 06:33:41 2002



From: Bill Brady :      wmbrady-at-olg.com
Date: Sat, 1 Jun 2002 07:22:42 -0400
Subject: Re: [Microscopes] basic stains and chemicals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rooting Wrote:

} For Formaldehyde/Formalin, are there any replacements which are
} non-flammable?

I use "Quick Cure" for aquarium use. Works fine in most cases. It's main
ingredient is Formalin.
}
} How good is frutose for a mounting solution?

See:
http://www.microscopy-uk.org.uk/larry/sugar0.html

Wm. "Bill" Brady, Harwood MD 38°51'30"N 76°41'00"W - Its in the darkest
hour that the most stars come out.



From daemon Sat Jun 1 14:11:02 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sat, 1 Jun 2002 13:08:58 -0600
Subject: Re: Manipulation of images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Manipulation of images is an issue that not only comes up in legal
proceedings, but in many other settings as well. One area is Pharmaceutical
research. There are very strict standards that the FDA sets regarding data
manipulation. Lab books have to be kept in certain ways to ensure that any
manipulation of data (legitimate or not) is traceable.
A few years ago, the FDA published a document (CFR21 rule 11), which
describes how a digital equivalent of a lab book has to be kept.
Unfortunately this document mixes applications, Operating systems, hardware
and SOPs, so it is very hard to implement (ask me off-line how we do that).
The essence is, that a document has to be defined as an "original" at some
point, as close as possible to the origin of the data. For a digital image,
this would probably be the time it is transferred to the computer and
displayed for the first time. ANY change to the data afterwards needs to be
documented and signed. For example, if you run a filter on the image, it
must have an audit trail and you must be able to go back to the original
image. That's a tall order for images, but it can be done. This kind of
audit trail would probably also stand in legal proceedings.

I don't think, that even this is a 100% insurance against malicious intend.
After all, you could theoretically change something in the camera
electronics, which would change the image before it was declared an original
document, but it comes pretty close.

mike

} } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Ave #300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Michael Cammer [mailto:cammer-at-aecom.yu.edu]
Sent: Friday, May 31, 2002 9:31 PM
To: Garber, Charles A.
Cc: MICROSCOPY BB


The most basic rule is that the controls and experimentals must be
treated the same before they are imaged and that they are imaged by the
same parameters.
Following the simple rules of doing valid controls and
taking the raw data in a uniform manner is far more critical than
the post-collection manipulation.

And the problems with the image manipulation are not that images are
altered intentionally, but that imaging amateurs (who may be the most
brilliant
geneticists or endocrinologists), who don't understand digital
representations, misuse Photoshop. A very common example is the misuse of
the Auto button in the Levels or Curves menu when rescaling from 12 bits
to 8 bits or to-byte-as-shown in any of the imaging packages. And then
the question we get is how to fix the problem, not to
compound it. "Why do the WT and KO look the same on the computer when I
swear the WT looked at least 2 times brighter through the eyepiece and the
Westerns show a 10X reduction in expression in the KO?"

People who want to cheat will always find a way to do so. The real
problem is educating to avoid simple imaging errors.


-Michael

___________________________________
WORK: http://www.aecom.yu.edu/aif/




From daemon Sun Jun 2 10:09:45 2002



From: Jackie :      ujtxh-at-eszett.de
Date: Sun, 02 Jun 2002 07:49:55 +0900
Subject: Great shape for summer,get there now

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Mon Jun 3 08:22:19 2002



From: William Oxberry :      William_Oxberry-at-downstate.edu
Date: Mon, 3 Jun 2002 09:08:16 -0400
Subject: Re:TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Donna,

I recently looked into getting a digital camera for my Zeiss TEM. At a
cost of $40k-$50k I decided to stick with 4x3 1/4 film and D-19 development
(archival negs) and digitize with a good scanner (Epson perfection 2450
about $400). I can get decent prints on a laser printer or my new Epson
C80 bj printer. This printer costs $150 and is amazing. If you need faster
output printing you can buy a dedicated computer, a couple of these
scanners and several printers and still spend less than $5k and have enough
paper for a couple years. At a savings of $35k my dept chair was happy

Bill




From daemon Mon Jun 3 08:32:17 2002



From: James Hayden :      jhayden-at-wistar.upenn.edu
Date: Mon, 3 Jun 2002 09:26:23 -0400
Subject: Ethics of Digital Manipulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This seems as good a time as any to put in my $.02.

Unfortunately, my answer would go for pages. I wrote an article on the
subject a few years back aimed at photographers and graphic artists that
may or may not be aware of the issues, especially in regard to
scientific images. It was published in the Journal of Biocommunications
and later, as an update in the Network journal of the Australian
Institute of Medical and Biological Illustration. This second paper is
reproduced (with permission) on my web site at:

http://www.biographics.org/pages/ethics.html

The paper was designed to make people aware of the issue and has a few
examples of what I termed "acceptable" and "unacceptable" manipulations,
along with information from a German case of particularly blatant
manipulation of images.

I just began a new position at The Wistar Institute in Philadelphia and
part of my mandate is to introduce the topic into required ethical
training here. This is a topic that will be with us for a long time. Any
comments on-line or off would be greatly appreciated. This thread is
certainly a good start.

Jamie Hayden

*********************************
James E. Hayden, RBP, FBCA
Manager: Microscopy Core Facility
The Wistar Institute
Room B-78
3601 Spruce Street
Philadelphia, PA 19104

office phone: (215)898-3887
cell phone: (215)514-4223
email: jhayden-at-wistar.upenn.edu



From daemon Mon Jun 3 08:58:52 2002



From: sjb27-at-cornell.edu
Date: Mon, 3 Jun 2002 09:52:29 -0400 (EDT)
Subject: LM Need cutting wheel specs for LKB Histo Knifemaker 2078

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Recently received a used LKB Histo Knifemaker 2078
(makes Ralph glass knives, not triangular glass knives)
but need to replace the cutting/scoring wheel. The
manual does not list the specifications of the wheel,
and the company that made the Knifemaker is no longer
in business making knifemakers.

Does anyone know the specs, or know a supplier for the
cutting wheels?

Thanks,
Sandra Borgardt
L. H. Bailey Hortorium
462 Mann Library
Cornell University
Ithaca, NY 14853


From daemon Mon Jun 3 08:59:36 2002



From: mail.thelinks.com :      sstouden-at-thelinks.com
Date: Mon, 03 Jun 2002 08:57:57 -0500
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



It has been my experience in expert testimony, that things like images need
expert testimony.
Have you seen the image that is on this media(cross examiner holds media
and image in hand)? Did you take the image? Is this the image you made? Are
you sure? How many other images did you make in the course of doing this
work? What happened to them? Did you modify the image in any way? IF so,
what were the nature and extent of those modifications? Are those types of
modifications normal practice in your industry, in your lab, for or by you?
Did you do the work yourself or was a technican involved? If technican,
what is his/her name? Without the modifications, what would have been the
result? At any time during the time you started the work and ended the
work was the "object to be investigated" under your direct control? If
not, please explain.
Are the images reproducible at any time by anyone with your level of training?
What type of equipment did you use? What is the best equipment that could
have been used to make this image? What did you not use that? Were you
limited by availability in your lab? If so, would that have affected the
result?
Ok, now in your opinion what does the image labeled "such and such" on
the "media" you provided the court, the one I am holding up for the jury to
see now mean to you? ... your answer.... Is your answer subject to any
kind of doubt or question, if so, what is the nature of your doubt or
concerns? Did you use any assumptions in expressing your findings about
this image? If so, what were they?
In your opinion as a professional the image shows " that the bullet that
killed the Deer was fired from this weapon [holding it up]...at such and
such a time on such and such a date?" Is that correct?

(be careful, you can only testify that the bullet was fired from the weapon
on a date and time, not that the bullet killed the deer unless you made
that determination) Thank you.


At 08:44 AM 5/31/2002 -0400, Ken Converse wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jun 3 09:25:58 2002



From: Brad Storey :      storey-at-lanl.gov
Date: Mon, 03 Jun 2002 08:19:10 -0600
Subject: EM Position at Los Alamos

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Los Alamos National Lab has an opening for a electron microscopist to work
in the Nuclear Materials Science group (NMT-16) on Pu alloys and other
actinides.

Applicants must apply directly through the official LANL web site, but they
may contact Brad Storey at storey-at-lanl.gov or 505-667-0458 with questions.
Note that this position requires US citizenship.

Summary:
The Nuclear Materials Science Group, NMT-16, of the Nuclear Materials
Technology Division is seeking a highly motivated materials scientist to
study plutonium and other actinide materials. The successful candidate
will participate in a team environment in ongoing experiments to support
Pit Surveillance, Pit Manufacturing, and Pit Certification Programs. The
primary experimental focus will be in fielding and advancing
state-of-the-art materials electron microscopy to investigate production
problems, component failures, and aging stockpile issues. They will have
access to three newly acquired instruments: a JEOL 6700F field-emission gun
SEM (WDS and EDS); a JEOL JAMP-7830 field-emission gun Auger Microprobe
(Auger, XPS, cold fracture, heating, orientation imaging microscopy, gas
reaction, AFM/STM); and a Kratos Axis-Ultra imaging XPS system (XPS, Auger,
heating/cooling, gas reaction). These instruments will be installed in the
Plutonium Facility at TA-55 in the spring of 2003, and enhance the
experimental capabilities of a well-equipped material science laboratory
that includes: a variable pressure SEM (EDS, WDS, OIM, CL spectroscopy,
hot/cold stage); a 5 spectrometer JEOL 8200 Electron Microprobe; diverse
x-ray diffraction capabilities; hardness testers; several modern optical
microscopes with digital cameras; and a nicely equipped metallography
line. The successful candidate will perform hands-on work with plutonium
and other actinides in a glovebox environment and mentor technicians in
materials science topics related to particular experiments.

Required Skills:
Extensive experience in materials characterization studies utilizing
electron-beam instrumentation and associated detectors, e.g., SEM,
microprobe, EDS, WDS, CL, OIM, etc. Recent peer-reviewed publications that
include SEM and microprobe as characterization tools. Strong knowledge of
quantification procedures relating to these techniques. Experience
maintaining state-of-the-art electron microscopy equipment and teaching
technicians and staff to operate it. Strong knowledge base in materials
science as evidenced by an advanced degree in the field and a
publication/presentation record. Demonstration of organizational skills and
good communication skills. Ability to obtain a Q clearance, which usually
requires US citizenship.

Desired Skills:
Experience in the design, production, or evaluation of nuclear weapon
systems and components. Ability to evaluate microstructures as they relate
to materials properties and processing history. Experience working with
nuclear materials in glove boxes, classified document handling at all
classification levels, and familiarity with quality assurance principles.
Experience writing successful proposals to secure funding. Active DOE
Q-clearance and PSAP approval.

Education:
MS (Ph.D. preferred) in materials science, nuclear, or metallurgical
engineering, or equivalent combination of education and experience.

Additional Requirements:
This position is subject to the requirements of the Personnel Security
Assurance Program (PSAP). All candidates invited for an interview must
consent to be in the PSAP program at the time of the interview. Only the
selected candidate will be subject to the requirements of the PSAP program,
which includes a pre-employment screening check, medical examination, and
drug test.




***************************************************
Brad Storey, Ph.D.
Team Leader, Microstructure & Microanalysis
Nuclear Materials Science Group (NMT-16)
Nuclear Materials Technology Division
Los Alamos National Lab
PO Box 1663, MS E574
Los Alamos, NM 87545

Tel.: 505-667-0458
FAX: 505-665-7815
pager: 505-996-3129
storey-at-lanl.gov
***************************************************




From daemon Mon Jun 3 09:49:22 2002



From: Greg Strout :      gstrout-at-ou.edu
Date: Mon, 03 Jun 2002 09:42:41 -0500
Subject: Ultracut Fluorescent bulbs

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Does anyone know what the specs are for replacement fluorescent bulbs on
a Reichert Ultracut microtome? The specs are not in the manual and our
bulbs are so old that the printing is illegible. TIA.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Mon Jun 3 11:24:26 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 03 Jun 2002 09:11:44 -0700
Subject: Re: SEM specimen holder

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Dear Heather,
I also use an S-570 for lots of different kinds of samples and I have got my
shop to make all sorts of clamps, jigs and pin-stub holders over the years.
I would think that a shallow groove to match the glass slide width could be
milled in the flat holder to just fit the slide. Either that, or a vise with
a fixed side and a sliding or spring-mounted side to close on the slide.
Just make sure it is made all of non-magnetic materials. Whatever you get
them to make just needs a 4M threaded hole in the bottom to take the stage
insert that screws into the bottom of your existing specimen holder.
At 03:55 PM 05/31/2002 -0500, you wrote:
} Hi Everyone,
}
} We are using an Hitachi S-570 SEM for examination of rock thin sections
} mounted on 1" x 3" glass microscope slides. Up until now, we have been
} securing the slides to a large circular (50 mm) specimen holder with
} copper and/or carbon tape. We would like a holder that would not require
} the use of tape, carbon paint, etc. Does anyone know if there are any
} commercially available holders (similar, for example, to that found in a
} Cameca Microprobe, where a metal frame secures and grounds the slide to
} the base) that would work in our microscope?
}
} Any design ideas would also be appreciated since our machine shop will
} fabricate what we need as long as nothing is commercially available.
}
} Thanks for any help,
}
} Heather Owen
}
} Dr. Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} Lapham Hall, P.O. Box 413
} Milwaukee, WI 53210
} USA
}
} Phone: (414)229-6816
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Mon Jun 3 11:30:37 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 3 Jun 2002 12:24:22 -0400
Subject: RE: AO & TB O Metachromasia and Science - Personal Reflections on

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Sergey,

In 1965, in a lecture by one of the virologists who worked on Flu
vaccines at Merck, Sharpe and Dhome(?) (West Point, PA), he mentioned that
early on before the discovery of the RNA nucleoids of certain viruses, AO
became briefly popular, because it differentiated between DNA and RNA
(strandedness not then an issue). For a while, green fluorescence in AO
stained cultures of DNA viruses was used to follow processes leading to
assembly and burst (CPE). Then, the axe fell. RNA viruses fluoresced green
during development. Very mixed up cytoplasm of infected cells since both
single and double-stranded RNA(?) appeared to be present. This was reported
to have been announced at a meeting (?) after a paper in which AO
fluorescence was part of the presentation. Why the problem? Well, one of
the more difficult problems in the study of any virus was getting enough
pure virus to work on. Most microbiologists then were NOT biochemists. The
use of AO in THAT context was ended. We had one of the newer Sorvall
Ultracentrifuges. Three people were permitted to use it, and most didn't
understand the principles of its operation!

Viruses were still "filterable agents".

Re: Foster R Jr, Metcalf D, Kirchmyer R., "Induction of
bone marrow colony-stimulating activity by a filterable agent in leukemic
and normal mouse serum". J Exp Med. 1968 May 1;127(5):853-66.

and Re: Cook MK.,"Cultivation of a filterable agent
associated with Marek's disease", J Natl Cancer Inst. 1969 Jul;43(1):203-12.


One key to understand is that in 1965, as far as we knew, there was
only one course in virology at the 300 level in the country. The book that
was used was a brief text that concentrated on phages and E. coli and
Hershey and Chase and, of course, the Warring Blender - at that moment in
time, also a very modern culinary machine, which my Mother wanted but had
not yet received for Xmas. Further, most undergraduate cell biology books
still hadn't incorporated Watson and Crick, except as recent historical
notes. Full incorporation took almost 20 years - ~1975.

Acta Microbiol Acad Sci Hung 1966;13(2):185-7, "Acridine orange
fluorescence of tissue cultures infected with Aujeszky's disease virus",
Bodon L, Greczi E.

The above reference was typical of reports of the use of AO in
virology. Nucleic acids, cell culture.

Of the great sexually-oriented processes then known: prophase,
leptonema, zygonema, pachynema, diplonema, diakinesis, metaphase, anaphase,
replication, transcription, translation, and osculation, only the last MIGHT
have been found on the S.A.T. It is problematic whether any of the last
four would have appeared on the graduate record exams of the time [I can't
remember!!!].

What might be called second-tier microbiologists around the world
were trying to learn the new jargon and techniques while being inundated
with NEWER jargon and techniques. Delbruck and Lauria were pushing the
envelope [and we couldn't keep track!], and they were applying the new
Watson and Crick dogma to microbiology and bacterial viruses, while others
were just beginning to investigate the 'inward workings' of the few animal
viruses that were known. The same virologist mentioned above, Richard
Malsberger at Lehigh, would demonstrate during the afternoon lab the
fundamental of what was known about Herpes simplex by initiating a full
eruption of a so-called "cold sore" on his lower lip by eating a piece of
DARK chocolate at the beginning of the 8:00am lecture the same morning [my
timing may be off after all these years!]. Question: would he do that
demo in his Immunology class today or would it still remain in Virology?

In five years at Lehigh, 3 or 4 graduate students took the virology
lab. We learned to do I.D. and L.D. 50's on mice using adapted Flu virus.
We learned hemagglutinin tests/assays, complement fixation, viral
titrations, 2-fold, 5-fold, 10-fold dilutions - things only graduate
students understood. We learned about Landsteiner, and began to feel that
only Physical chemists would ever do anything or understand anything. We
learned how to fail at cell culture using the wrong "distilled" water.
Most labs had water stills hanging on the walls in the prep rooms whose
boiler and copper cooling coils were plated with chromium. Deionizers were
modified/upgraded water softeners. If you cultured animal cells, you
cultured HeLa cells. We did CPE's and plaque assays. Those who taught and
learned were probably a decade behind those who were investigating and
reporting - perhaps little different from today.

A Leitz Ortholux with fluorite objectives, darkfield condenser and a
mercury arc illuminator with exciter and barrier filters, AND the Orthomat
fully automatic 35mm camera system with TWO film cassettes [one for color
(for fluorescence) and one for B&W (for bright field)] cost around
$6,700.00. This system was SOooo advanced that the senior graduate student
in virology had no experience with microscopy and misinterpreted the
instructions for oil immersion to suggest that the objective was to be
FILLED with oil (which he did!). The objective was returned to Germany for
cleaning. We made out own fluorescent antibody, first with Fluorescein and
then with FITC. The clonal selection theory (Burnet and others) was
something in a book that only graduate students in virology read. When I,
an anatomy student, was seen reading THAT book, it was observed that I was
wasting my time. When I took microbial biochemistry, I was considered wise
by some and as a reactionary by others.

RCA made electron microscopes and they were NOT found
everywhere! The ultramicrotome of the day was the MT-1, and the NEW, magic,
motorized MT-2! We had just received the just released those magic
balances by Mettler. Oh, to weigh a gram in a minute to 2 places of
precision! [I now have a double pan balance of my youth as "...the largest
brick-a-brack in our house".]

World War II had been over for 20 years and the transistor had just
found its way into affordable, portable radios - AM AND FM! And then, of
course there was SPUTNIK and Gagerin and the Moon! Electrophoresis was on
purified cellulose paper! Total nitrogen by Kjeldahl! Amphoteric amino
acids by acid titration, deflections, and chart recording. A Frieden
mechanical calculator with twenty columns of numbers "clunkety, clunkety,
clunkety" - - off the lab bench onto the floor - - - "clunkety, clunkety,
clunkety" - - - all through the lunch hour - - "clunkety, clunkety" - - -
lying on its side, on the laboratory floor. A magic computer in the
basement [all of it!] of the Engineering College". Stacks of cards. Boxes
of stacks.

While there were some during this time who preferred being stoned
[they get all the publicity now!], most were sober and trying to soak it all
up and get on for the ride. I grew up 3 miles from Bell Labs in Berkely
Heights (Murray Hill), NJ and 15 miles from Princeton and saw Prof. Albert
walking on the street one day as our 54 Chevy passed through town and met
two of the transistor guys at a party in Summit, NJ sometime later. We were
taught in the 50's that only three or four people understood what Prof.
Albert presented in his benchmark papers in the 20's. Relativity had more
social meaning, even then, than physical.

In any case, back to AO. As I note the history of virology now, the
use of AO to study virus infections then is not even given the space of a
footnote in current histories. Not an undeserved relegation.

Sorry for the additional ramble,

Fred

} ----------
} From: Sergey Ryazantsev
} Sent: Thursday, May 30, 2002 7:10 PM
} To: Monson, Frederick C.; Microscopy-at-sparc5.microscopy.com
} Subject: Re: Toluidine Blue O - Metachromasia and Science
}
} Fred
}
} I don't understand your point about Acridine Orange (AO)? How discovery of
}
} the RNA-viruses affects the staining and our knowledge about this
} particular staining agent? As I remember, AO staining is based on polar
} interactions, mostly with "nucleic acids". The 'color' of fluorescence
} depends from the energy transfer efficiency between donor (AO) and
} acceptor
} (which may be, may be not nucleic acid). I expect, the 'color' from AO
} stained double strands RNA would be similar to DNA. Another point here,
} as
} far as I know, RNA viruses in most cases contain "double-stranded RNA
} segments", so it's a mixture single/double-stranded RNA. I could not
} predict how it will fluorescence with AO. Nevertheless, such viruses are
} very small and I don't believe you could even see their fluorescence with
} AO on the autofluorescence background of the typical cell. The bottom
} line
} here: the discovery of the new "RNA-viruses" is not affected our knowledge
}
} of basic properties of AO and AO would work (I believe) at the same manner
}
} with this 'double-stranded RNA' as with other stuff. I don't see big
} difference between single/double-stranded-RNA and DNA. It's well known,
} that most RNAs tends to form secondary structure (call it double-stranded
} RNA fragments) under the physiological conditions. Moreover, my personal
} experience shows that it's extremely difficult to make RNA completely
} 'single-stranded': even at the presence of 8MUrea+formamide,
}
} Sergey
}
}
}
} At 09:56 AM 5/30/02, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Morning All,
} }
} } For those among you who are wise, you should probably
} immediately
} } jump or GOTO END!
} }
} } This is difficult to put into words without chancing a gross
} error
} } or causing some offence, but here goes anyway.
} }
} } When we "fix" a specimen taken from a biological source, we
} NEVER
} } quite know what we are doing. The entire process is 'outcome-based'
} } (Ugh!!!). How does it look? Does the fixative 'coagulate' or
} 'cross-link'
} } the myriad of chemical entities within the sample? What is the effect of
} } specimen size? What about temperature? How long to kill? How long to
} } achieve minimum preservation? Preservation of what? What will
} constitute
} } comparable samples that permit statistical comparison of specimens of
} mouse
} } and elephant liver? We preserve or fix or kill or maintain color or get
} rid
} } of it. Behind every act there lies a reason, and in consequence of every
} } act there is produced an uncountable number of variables which only
} } REPRESENT what existED in the original, living, organism. Everyone says
} } that s/he already knows this!
} }
} } Metachromasia is part physical chemistry, part biochemistry (and
} by
} } reason of those two, for many, little more than pure magic!).
} Metachromasia
} } has been extensively used, extensively studied and extensively reported
} } upon. Several items of information are clear [This may be an
} } exaggeration!].
} }
} } 1. The polychrome effect noted in the use of several related
} dyes
} } of the thiazine (quinone-imine/methylene blue) family is based on the
} } polymerization of dye molecules and the distances between adjacent acidic
} } groups on the substrate.
} }
} } 2. A loss of the polychrome effect could be attributed to*:
} } a. decrease in the concentration of some
} tissue/substrate
} } component
} } b. decarboxylation [- or - esterification with
} alcohol?]
} } c. degradation of the substrate to diffusible
} constituents.
} }
} } [* see Pearse, A.G.E.(1985), Histochemistry, Theoretical
} and
} } Applied(4th Ed.), Vol II, Churchill Livingstone, NY,NY, pp701-710. ISBN:
} } 0-443-02997-0.
} }
} } 3. In order for the metachromatic color. for (Toluidine Blue O,
} } that is purple [red + blue]) to be observed, the appropriately spaced
} acid
} } moieties must be deprotonated, i.e. negative in charge. Otherwise, the
} } orthochromatic color is observed.
} }
} } In other words, in order for there to be metachromasia of a
} } particular component of the tissue, that component must have
} appropriately
} } spaced negatively charged groups.
} }
} } 4. At pH 7, most 'acid' moieties in biological systems are
} } deprotonated, and thus, negatively charged. The isoelectric points of
} most
} } macromolecules are in the vicinity of pH 5 [This IS an exaggeration!]
} Where
} } pH can determine dye binding, pH can be used to partition objects in the
} } specimen space along a pH gradient [also in Pearse, same pages, but see
} } Methylene Blue Extinction (MBE) methods in many compendia]. [MBE used to
} } distinguish among histologic 'acid' mucosubstances (GAG's, etc.).]
} }
} } 5. A buffer may stabilize substances, react with them, or
} promote
} } changes in them (i.e. oxidation).
} }
} } 6. A bottle of dye, used for 5 years to produce a result, may,
} at
} } some moment, STOP behaving as it should. Pearse mentions that one of the
} } reasons that metachromasia was such a confused subject prior to the
} 50-60's,
} } was due to the fact that so many studies failed to use pure dyes.
} }
} } RULE: If a dye fails to function as it should. Try a different batch or
} } make up a fresh batch, or purchase a new supply. I have found that a
} simple
} } 0.1% solution of the dye gives rise to regular, reproducible
} metachromasia
} } which survives dehydration in absolute ethanol, but never in 95% ethanol.
} } That having been said, when I have performed the Azure B, pH 4.0 for
} } ethanol-acetic acid(3:1)(Clark) fixed nucleic acids (Flax and Himes,
} 1952),
} } I follow their protocol for dehydration and use tertiary butanol.
} } Preparations I made in the mid-60's still show metachromasia, albeit with
} } some overall loss of color in my personally prepared Damar-xylene
} mountant.
} }
} } The questions about the failure of any regularly used dyeing
} } procedure amount to a scientific challenge that are best addressed by the
} } one who is having the problem. Since the variables are many, the sources
} of
} } failure are also many. One has to learn how to perform a component
} analysis
} } in order to efficiently address such a problem. Since much of what one
} does
} } in dyeing/staining is by prescribed protocol, it should be clear that if
} } anything has changed, it is the operator who is in the best position to
} DO
} } the troubleshooting. When I ran the Flax and Himes procedure, I always
} } retained the blocks of previously sectioned material. Each sectioned
} block
} } was dipped in paraffin to cover the exposed tissue and stored carefully
} } away. I was especially careful of those specimens, prepared for any
} } particular purpose, in the event I ever required a 'known' tissue source
} of
} } a good result. Even so, I was aware that the stored specimens would not
} be
} } the same in two years as those I sectioned yesterday. I learned this
} when I
} } addressed the issue of saving tissues labeled with tritiated thymidine.
} } Why, I asked parenthetically, should I be able to determine that loss of
} } radioactivity by disintegration was not going to be augmented by loss due
} to
} } progressive destruction of DNA, if I had no specific knowledge of how
} much
} } DNA/nucleus/section was present in the starting material? So, I learned
} } that I would not be able to have compete faith, even in the best of my
} } archived specimens, 5 or 10 years in the future.
} }
} } NOTE: Acridine orange was one of the first fluorescent dyes
} used by
} } virologists in the late 50's/early 60's. One was able to distinguish
} } between single stranded RNA and double-stranded DNA until someone noted
} the,
} } then recent, discovery of the RNA viruses. "Nuts!" Even now, there is
} an
} } extensive literature on the use of AO fluorescence for double-dyeing the
} } nucleic acids in cell nuclei.
} }
} } An absolute obligation of old windbags is a SUMMARY: If there
} is a
} } single point in all of this, it is this. In the application of
} } metachromasia there are considerations of mass action, pH, purity of the
} } dye, and treatments preceding and following the application of the dye.
} If
} } each adds an order of magnitude to the number of variables involved,
} there
} } are 4-5 such ordinal magnifications through the process. If the physical
} } and chemical bases of histologic methods are understood at less than
} optimal
} } levels, then troubleshooting will be a problem that has little hope for
} } success. On the other hand, even one who has only the recipe to which
} s/he
} } can refer can be taught component analysis of that recipe.
} }
} } Mom used to say, "If you don't know what kind of flour to put in
} } your bread, try any flour and the bread will let you know if you were
} right
} } or wrong. If you were wrong, and really want bread, then you will have
} to
} } try another flour, and another, until the bread tells you that you are
} } finally right. Just don't change any other part of the recipe while you
} are
} } testing the flour." Ah! If only more of us had learned to bake when we
} } were young.
} }
} } END: Respectfully submitted,
} }
} } Fred Monson
} }
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging
} } Schmucker II Science Center
} } West Chester University
} } South Church Street and Rosedale
} } West Chester, Pennsylvania, USA, 19383
} } Phone: 610-738-0437
} } FAX: 610-738-0437
} } fmonson-at-wcupa.edu
} } CASI URL: http://darwin.wcupa.edu/casi/
} } WCUPA URL: http://www.wcupa.edu/
} } Visitors URL: http://www.wcupa.edu/_visitors/
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}


From daemon Mon Jun 3 11:50:24 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 3 Jun 2002 12:43:30 -0400
Subject: Legal Image Standards???

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Hi All,

I don't know much about this other than it exists as an issue in government
circles such as those found revolving at FDA and NCI.

URL: http://www3.cancer.gov/scienceresources/announcements/imaging.html

URL: http://www.acuotech.com/fda_filing.asp

Interesting Commercial Site: http://www.archivebuilders.com/

and also see:
http://www.archivebuilders.com/whitepapers/22041p.pdf

What constitutes a 'legal' digital, archived image has become a VERY
interesting topic.

Have a good one,

Fred

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Mon Jun 3 12:23:13 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Mon, 3 Jun 2002 13:12:15 -0400
Subject: Jeol 100C

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We have a JEOL 100C top entry scope for sale to anyone interested. Has 2
sets of plate film boxes and holders. Asking $10,000 or Best offer with
removal and shipping at buyers labor and cost. Scope has been under service
contract for the last 10 years.

Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Mon Jun 3 12:29:42 2002



From: Mike McKay :      mike.mckay-at-vitana.com
Date: Mon, 3 Jun 2002 13:21:06 -0400
Subject: RE: TEM-Digital Camera Systems

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Dear Ms. Clarkson,

I represent Vitana Corp., a digital camera manufacturer based in Ottawa,
Canada. We manufacture the PixeLINK brand of megapixel FireWire cameras
aimed at the scientific and industrial imaging markets.

At $1695 USD, our monochrome 1.3 megapixel camera may be of interest to you.
PixeLINK cameras provide excellent value and could act as a megapixel
preview system in support of a higher-end camera. We also manufacture a
color version priced at $1795. Please visit our web site
(http://www.pixelink.com) for more detailed information.

To answer your questions,

* What make & model camera system do you have?

The PixeLINK PL-A641 Monochrome camera is a 1.3 megapixel camera, connected
to a computer with a single FireWire cable. There are no other cables,
framegrabbers, nor power supplies. The camera uses a CMOS sensor and is
well suited to brightfield applications. The camera body mounts to the
photomultiplier or video coupler via a standard "C"-mount. For more
information on the cameras, please visit the product page on our web site -
http://www.pixelink.com/products/600.htm.

* Do you feel you get micrograph-quality resolution and images
utilizing that system?

Uncooled and with 1.3 megapixels and 10 bit sensitivity, the PixeLINK
cameras will not provide comparable images to photographic systems. The
comparison would have to be in terms of cost, speed and ease of use.

* Ease of use--is it user-friendly or cumbersome?

PixeLINK products are designed to be user-friendly. The camera equipment
and software can be installed and functional in just a few minutes. The
PixeLINK Capture application allows full control of the image capture
process. Exposure, brightness, and gamma are all easily adjusted.
Darkfield and brightfield corrections can also be applied. Images can be
saved, sequential numbered, in TIFF or bitmap formats. A bitmap overlay can
be applied to the image. Time-lapse operation is possible and the camera
can be operated with a remote control foot switch.

The most useful benefit to the user is the real-time preview. A full-screen
preview (1280x1024) at up to 14 frames per second can be used to observe the
specimen prior to image capture. At the more common VGA resolution
(640x480), the speed increases to 30 frames a second. At these speeds,
there is little need to examine the image through the eyepiece of the
microscope. The result in increased productivity for the operator.

* Ease and/or quality of service--are any of the components
serviceable by you? How quickly & easily is it to get a service rep?

The camera requires little support or service. Installation is simple and
operations training is not required. For support, call us at 1-800-4VITANA
(1-800-484-8262).

I'd be happy to answer any other questions you may have.

Yours,

Michael McKay

{ { { { { { { { { { { { { { { { { { { { { { { P i x e L I N K } } } } } } } } } } } } } } } } } } } } } } } } }
Michael McKay, Product Manager {mailto:mike.mckay-at-vitana.com}
Vitana Corporation
2500 Don Reid Drive Tel: (613) 247-1211 x 152
Ottawa, Ontario Cell: (613) 859-6174
Canada K1H 1E1 Fax: (613) 247-2001
"Making Digital Imaging Simple {http://www.pixelink.com} "
{ { { { { { { { { { { { { { { { { { { { { { { P i x e L I N K } } } } } } } } } } } } } } } } } } } } } } } } }



From daemon Mon Jun 3 12:33:16 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Mon, 3 Jun 2002 13:23:37 -0400
Subject: Jeol 100C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have a JEOL 100C top entry scope that is looking for a new home for
anyone interested. It has 2 sets of plate film boxes and holders. Asking
$10,000 or BO with removal and shipping at labor and cost. Scope has been
under service contract for the last 10 years.

Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Mon Jun 3 12:50:23 2002



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Mon, 03 Jun 2002 13:40:28 -0400
Subject: Re: Ultracut Fluorescent bulbs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Reichert Ultracut E and it takes a Philips TL 4W/33 XD6 or an
OsramL 4W/ 25, Weiss-universal-white. You might try Bulbman,
1-800-648-1163, or www.bulbman.com if you can't find them locally.
Mary Gail Engle

At 09:42 AM 6/3/02 -0500, Greg Strout wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-9700


From daemon Mon Jun 3 12:53:40 2002



From: Jeff Thompson :      jthompso-at-csusb.edu
Date: Mon, 03 Jun 2002 10:45:13 -0700
Subject: Auto Film Processor for EM negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are evaluating an automatic film processor for developing EM
negatives from AFP Imaging Corp. (Mini-Medical series). We are trying
to develop SO-163 EM negative film with D-19 developer. No problem when
we develop manually in a tank. In the new processor, the film comes out
foggy/milky in a botchy pattern at most temperatures with a development
time of 130 seconds. If the temperature is maintained at exactly 85.6 F
then the film is clear. A second problem is that we see what we think
are roller marks on the film. A final note: the processor works fine
with X-ray film (Kodak-AR) for use in autoradiography.

My questions: Is anyone using a automatic processor for EM negatives
successfully?
Do you have any suggestions for tweaking the AFP processor?
Is there something special about the emulsion and/or gelatin coating on
the SO-163 film?

Thanks.

Jeff Thompson
Director, Electron Microscope and Image Analysis Center
Department of Biology
California State University
San Bernardino, CA 92407
909-880-5315



From daemon Mon Jun 3 13:20:32 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 3 Jun 2002 14:13:35 -0400
Subject: FDA and Electronic Documents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Afternoon Listers,
I thought that the information/sites listed herein might be of
interest to many of you, so here it is.

I have been aware of the issue of "acceptability" in electronic
image/document storage and archiving. What I have not done until now is
search out the sources of useful information so that the issue comes into
greater focus, so to speak. While the sites listed below are United States
Government sites, the issues, as seen by the government regulators, are
exposed, at least in part. The most important thing is to note the
complexity of the issues as summarized in the first site. Hope this is a
welcome sort of sharing.

See a long summary of the FDA guidelines/requirements at:

URL: http://www.devicelink.com/mddi/archive/99/05/009.html

FDA Rule: 21 CFR 11 published on 20 Mar, 1997 is the relevant publication.
It considers more than just images.

At the FDA site of the Center for Devices and Radiologic Health, one can
read: "Guidance for the Submission Of Premarket Notifications for Medical
Image Management Devices"

URL: http://www.fda.gov/cdrh/ode/guidance/416.html (under "Diagnostic
Imaging" or "Picture Archiving and Communications Systems (PACS)") in the
following FDA Index page.
URL:
http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfTopic/topicindex/topindx
.cfm
or for the PDF: http://www.fda.gov/cdrh/ode/guidance/416.pdf
or for the printed document: call: 1-800-899-0381 and ask for
document 416.


I will know if this is OK with most listers if I don't receive a torrent of
negative responses.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Mon Jun 3 13:38:56 2002



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Mon, 03 Jun 2002 13:34:37 -0500
Subject: Re:TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill

What about the cost of film and chemicals over the lifetime of the
microscope? As a one off charge your dept chair was happy but the
additional costs of dealing with film are not small. Also as a totally
digital materials science lab the main additional benefit is being able to
see immediately the image and know it is in focus etc without wasting film
and time.

Alan

At 09:08 AM 6/3/2002 -0400, William Oxberry wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Mon Jun 3 14:04:38 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 3 Jun 2002 19:57:03 +0100 (GMT Daylight Time)
Subject: Re: Ultracut Fluorescent bulbs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On my Ultracut E the writing is:
OSRAM (manufactuerer) L 4W/25

Interestingly my spare (unneeded in 13yrs here) is:
OSRAM L 4W/23

Dave


On Mon, 03 Jun 2002 09:42:41 -0500 Greg Strout
{gstrout-at-ou.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone know what the specs are for replacement fluorescent bulbs on
} a Reichert Ultracut microtome? The specs are not in the manual and our
} bulbs are so old that the printing is illegible. TIA.
}
} --
} ==================================================================
} Greg Strout
} Electron Microscopist, University of Oklahoma
} WWW Virtual Library for Microscopy:
} http://www.ou.edu/research/electron/www-vl/
} e-mail: gstrout-at-ou.edu
} Opinions expressed herein are mine and not necessarily those of
} the University of Oklahoma
} ==================================================================
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Jun 3 14:33:24 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 3 Jun 2002 20:26:27 +0100 (GMT Daylight Time)
Subject: Re: LM Need cutting wheel specs for LKB Histo Knifemaker 2078

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the UK we can get LKB spares from Leica. Perhaps they
can help.

Dave


On Mon, 3 Jun 2002 09:52:29 -0400 (EDT)
"sjb27-at-cornell.edu"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Recently received a used LKB Histo Knifemaker 2078
} (makes Ralph glass knives, not triangular glass knives)
} but need to replace the cutting/scoring wheel. The
} manual does not list the specifications of the wheel,
} and the company that made the Knifemaker is no longer
} in business making knifemakers.
}
} Does anyone know the specs, or know a supplier for the
} cutting wheels?
}
} Thanks,
} Sandra Borgardt
} L. H. Bailey Hortorium
} 462 Mann Library
} Cornell University
} Ithaca, NY 14853
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Jun 3 19:48:17 2002



From: Mark YEADON :      m-yeadon-at-imre.org.sg
Date: Tue, 4 Jun 2002 08:43:43 +0800
Subject: Postdoc, In-situ TEM, Growth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I would be grateful if you could bring this job ad to the attention of
anyone whom you think may be interested.

Many thanks,

Mark

Research Associate: In-situ TEM, Crystal Growth

The Institute of Materials Research and Engineering (IMRE) is seeking a
Postdoctoral Research Associate with a strong background in transmission
electron microscopy. The candidate will be involved in the in-situ growth
and characterisation of thin films and nanostructured materials using the
newly commissioned MERLION system, a modified JEOL 200kV TEM with ultrahigh
vacuum column. The system is equipped with in-situ electron beam
evaporators and gas injectors enabling real-time in-situ observations of
materials growth. The system is also equipped with a Gatan Image Filter
(GIF) and Dualview camera system.

IMRE is supported by the Singapore Agency for Science, Technology and
Research (A*STAR) and is situated on the campus of the National University
of Singapore (NUS). (www.imre.org.sg; www.nus.edu.sg).

Interested candidates should submit a comprehensive CV/resume together with
the names of at least 3 referees, to the address below. Email
correspondence is encouraged, and further details can be supplied upon
request.

=================
Dr Mark Yeadon
Institute of Materials Research and Engineering
3 Research Link, Singapore 117602

Tel: (+65) 6874 8591
FAX: (+65) 6872 0785
Email: m-yeadon-at-imre.org.sg
=================

%%%%%%%%%%%%%%%%%%
Mark Yeadon

President, Microscopy Society of Singapore

Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119260
http://www.matsci.nus.edu.sg/STAFF/Mark.html

TEL: (+65) 6874 8591
FAX: (+65) 6872 0785
Email: m-yeadon-at-imre.org.sg



From daemon Mon Jun 3 23:10:52 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 03 Jun 2002 21:02:18 -0700
Subject: Re:TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Using digital camera (600W BioScan, Gatan) with TEM over last couple of
month I find the following.

-most people prefer to use digital imaging even we offer equal access to
the film as well. It's because in most cases people need to evaluate
sample quality etc, they simply don't need film quality. From another hand
they could use film anytime if they wanted.

-with digital images you have chance to see your sample immediately
(important for evaluation/students in rush/deadlines etc).

-If you do not enlarge the digital image, 7x7" draft printout on laser
printer (standard) is looks very similar to the image produced from scanned
film. Manipulating with image resolution, dpi etc, you should keep in
mind, that 1200 dpi printer resolution mean that printer could produce 1200
dots per inch in the row making solid line. In order to create shadows of
gray, printer should print dots with space. For instance, 50% of grey
would be represented by 'dot-space' sequence. It mean, that resolution
would be 50% from 1200 = 600 dpi. For 30% gray, it would be 400 dpi. So,
there is no reason to abuse printer sending 1200 dpi 50 Mb image. On
practice, I could not recognize difference between 1x1K at 144 dpi digital
camera image from scanned from film 1200 dpi image: On laserJet (similarly
on our Tektronix dye-sub) - they are looks very the same. BUT: the
difference is that you could enlarge 1200 dpi image (and print with the
same quality) and you COULDN'T do so with 1x1K image from digital
camera. Another remark here: in 'prestigious' magazines like
Science/Nature technical editors have tendency to reduce image to the
postal stamp size, so you could do it by yourself and there is no need to
use film for such small images, digital camera will work just fine!

-another advantage of the digital camera, which, actually, I did not
expect, is its sensitivity. My camera is at least x10 more sensitive than
film, so exposure time is 0.1-0.2 sec versus my usual 1.5-2 sec for the
film, So, less drift and sample damage.

-another things are educational. Instead keep students in the dark and
teach them how to adjust binoculars (instead doing actual EM), we are
comfortably sitting around the 19" flat screen monitor with lights ON (and
nice, not loud classic music). I find it's more convenient to show how to
focus image on the computer screen rather than make a 'focus series', then
develop/print it. At this point students usually don't remember how image
was looks like on the microscope's screen and we have to start again. I
don't mean that students don't need to know how to operate
microscope/binoculars, I just mean that digital camera makes this process
more enjoyable and creative.

-another thing is academic: The live image from digital camera I broadcast
to the Departmental network, so you could see live image on any
departmental computer. PIs now comfortably sit in their offices and enjoy
watching how their students working on the TEM. Similarly it works for
Internet. We did a few session when our collaborators observe their
samples sitting far-far away from UCLA.

So, the bottom line is that TEM digital camera is very convenient
'supplement' to your EM. It does not replace the film, but enhance your
microscope's abilities. It's like power-steering in the car: convenient,
but not necessary - you may park your car in NY without this 'supplement'
right? As a matter of fact, cars without power-steering is cheaper.

Sergey

P.S. I just figured out that this message may be recognized as an
'advertisement'. So, it looks like I was working hard writing on ESL for
manufacturers. I would like to declare openly - I did not intend to do so
and nobody from Gatan or other manufacturer offered to me dinner or coffee
when I was writing it. I did it in sincere believe of what I was writing.

At 11:34 AM 6/3/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Jun 4 03:54:27 2002



From: Janos Labar :      labar-at-mfa.kfki.hu
Date: Tue, 4 Jun 2002 10:45:29 +0200
Subject: Evaluation of SAED ring patterns from nano-phase materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List Members,

I am happy to inform you that a brand new version (V2.0.7) of my free
program is now available at the same web-site where the previous version was
published
(www.mfa.kfki.hu/~labar/ProcDif.htm ).

Beside the new Multiple Document User Interface, new functionality has also
come: Some of the new features:

1/ You can generate Marker files on your own for known structures.
The new Structure Definition Module allows you to enter the
crystallographic data for known phases.
Automatic listing of the possible choices and some checking of the
consistency of the entered data are provided by the program.
(e.g. only the Space Groups in accordance with the selected Bravais
lattice are listed, Wyckoff positions for the selected Space Group are only
listed, and
a warning is given if the specified atomic coordinates are conflicting
with the multiplicity of the selected Wyckoff symbol)

Kinematic electron scattering (powder diffraction) data are calculated
to be used as Markers for the measured ring-patterns
(in contrast to using only X-ray data from a database, which is still a
valid option if the structure of the pahse is not known).

2/ You can copy the curves of the processed SAED patterns into 5 different
"Compare Memories". These data can be saved and later re-loaded.
Using this feature, you can compare distributions of intensities at any
time, without a need for later re-processing of previously processed SAED
patterns.

Compared distributions can be normalized on display to the same
full-scale.

3/ XRD data can also be loaded into Compare Memories for comparison with
SAED data. For this, the XRD data should be saved in a text file with the
following format:
2 columns, separated by coma. The first column is 2-Theta and the second
is Intensity (Counts).
A special 3-line header must be added:
"[Source = XRD]"
"[X-axis type = 2Theta]"
"[Lambda = 1.541]"
Obviously, the value of the wavelength should correspond to the true
value used in the XRD.
The header-format must be strict (case-sensitive,
character-by-character).

4/ New command buttons help stretch, compress and shift distributions in
either X or Y directions. The more accurate setting-possibility of the axes
still persists.

5/ New image formats are supported:
TIF (uncompressed) and RAW.
Both of them can be either 8-bit or 16-bit. Obviously, the point is in
using the 16-bit format, since the 8-bit format does not offer more that the
BMP-files.

6/ A simple Document is added to facilitate writing reports. It can save
text and pictures in Rich Text Format (.RTF files)
that can be read e.g. by MS Word for more sophisticated formatting.
Be aware that RTF files with embedded pictures are huge and saving them
takes a very long time.

{Alt} {Prt SC} can be used to copy shots of screen onto the Clipboard
from where you can insert them into the Document by {Ctrl} V.

Also see the new menu points for saving or copying graphs.

7/ Processing of negatives is done in two steps. First Select menu Process /
Use Inverted ... (this is a toggle-switch), then the usual Process /
Calculate Distribution.

8/ Option menu point helps you to determine
- where to find and store different types of files
- whether to obtain hints at every stage of your work
- what line-thickness to use in Schematic half-circles
- which minimal intensity is to be used to display Markers (later
versions will contain a possibility to show forbidden lines, too).

Some Folders are created for you as a suggestion where to store SAED
patterns, Structures, XRD Markers, Electron Diffraction Markers (EDM), etc.
You can change this as you wish. The program warns you if a non-existent
Path is specified as Default and offers the usual Windows interface
to select a new one, instead.

9/ Letter size is increased to produce better material for printing or for
Projected Presentations.

10/ Peak Search is separated with the inclusion of the possibility to change
the filter-parameters for search.
The window comes up with suggested parameters, but you can change them
to experiment how to find most peaks with less noise.
Peak positions hopefully became more accurate, but do not underestimate
the importance of visual inspection.

11/ I hope the program became more robust. However, I would appreciate
receiving any comments, suggestions or reports on errors.

I wish you good luck in using this program.

Best regards:

Janos L. Labar




From daemon Tue Jun 4 06:14:39 2002



From: Susan Carbyn :      CarbynS-at-agr.gc.ca
Date: Tue, 04 Jun 2002 07:05:26 -0400
Subject: Re: Ultracut Fluorescent bulbs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Greg,

Our Reichert Ultracut E Microtome has Philips TL4W/33 F7 bulbs in it. Sounds like your bulbs lasted a long time, since you can't read the print. We haven't had to replace ours yet, so I guess they must last awhile.

Susan

Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, N.S. B4N 1J5
Canada

Phone (902) 679-5535
Fax (902) 679-2311

E-Mail: carbyns-at-em.agr.ca

} } } Greg Strout {gstrout-at-ou.edu} 06/03/02 11:42AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anyone know what the specs are for replacement fluorescent bulbs on
a Reichert Ultracut microtome? The specs are not in the manual and our
bulbs are so old that the printing is illegible. TIA.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================






From daemon Tue Jun 4 06:22:51 2002



From: LLOYD, PAMELA F [AG/1000] :      pamela.f.lloyd-at-Monsanto.com
Date: Tue, 4 Jun 2002 07:16:40 -0500
Subject: Ultracut Fluorescent bulbs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Heather
A simple holder to make would be a flat piece of 4-6mm aluminium
same size as the slide or a little larger, drilled M4 in the centre,
fitted with non-ferrous metal spring clips similar to LM stage clips
Chris

Date sent: Fri, 31 May 2002 15:55:04 -0500 (CDT)
} From: Heather A Owen {owenha-at-csd.uwm.edu}
To: MSA Listserver {Microscopy-at-sparc5.microscopy.com}


Greg,

We have a Reichert/Leica Ultracut S. The fluorescent bulb is a 5W Osram
Dulux S/E. We get it from Mager Scientific Inc. in Dexter, MI. The part
number is 870041.

I don't know if this is interchangeable with the Ultracut E.

Pam Lloyd


Pamela F. Lloyd
Research Associate
Monsanto Co.
800 N. Lindbergh Blvd.
U1E
St. Louis, MO 63167
Phone: (314)694-6527
FAX: (314)694-8065
e-mail: pamela.f.lloyd-at-monsanto.com

-----Original Message-----
} From: Greg Strout [mailto:gstrout-at-ou.edu]
Sent: Monday, June 03, 2002 9:43 AM
To: Microscopy listserve


Does anyone know what the specs are for replacement fluorescent bulbs on
a Reichert Ultracut microtome? The specs are not in the manual and our
bulbs are so old that the printing is illegible. TIA.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Tue Jun 4 08:01:12 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 4 Jun 2002 08:54:29 -0400
Subject: RE: Mitotic index - McAuliffe cont'd

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with Geoff, Augustin. Br-UdR is the current way to go. It actually
requires more work than 3H-TdR, but for short-term experiments, its use
avoids a LOT of safety and regulatory hassles. Problem is that in longer
term experiments, where 3H-TdR is known, by literature support, to 'breed
true', there appears to be less such support for long-term Br-UdR. If you
just count the layers (i.e., the number of steps to finished product),
3H-TdR comes out way ahead in resolution, ease of use and precision, BUT the
hassle in use of radioactive labels is almost totally limiting if one only
considers the added cost of handling the remains.

When I used 3H-TdR on rabbit urinary bladder in situ(vivo) in the early
90's, I ended up using very low dosage per gram of body weight (~0.1uCi/g)
when I introduced the label i.v. and normal (for me!) dosage (0.5uCi/ml or
g) in aerated Hank's BSS (NO BSA added!!) for in vitro incubations of either
whole bladder or bladder strips. Labeling indices proved to be similar in
three categories: whole bladder labeled either in vivo(1) or in vitro(2)
and bladder strips in vitro.

NOTE: the throw-away radiation level was 0.05uCi/g body weight at that time
(early '90's) so even when dosed less and exposed in a special manner,
hazardous waste disposal was still required for the carcass. Needless to
say, the growing cost of disposal caused me to expend both energy and time
in developing a means by which I could dose with 0.049uCi/g body weight and
not have to wait for a generation (mine!) for the autoradiograms to expose.
Got close, but time and money ran out.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

} ----------
} From: Geoff McAuliffe
} Sent: Monday, June 3, 2002 4:58 PM
} To: Agustín Venzano
} Cc: HistoNet Server
} Subject: Re: Mitotic index
}
} Augustin:
}
} I believe that bromodeoxyuridine is a more accurate index of mitotic
} activity, but I can't remember the citation right now. Actually seeing
} mitotic
} figures is difficult, they don't last long! S-phase often lasts 10-12
} hours,
} mitosis lasts 20 minutes so incorporation of something into the DNA during
} S-phase is the way to go. The "old" way with tritiated thymidine
} autoradiography
} has problems due to disposal of animals and contaminated reagents.
}
} Agustín Venzano wrote:
}
} } Dear netters: I'm planning a sampling of ruminal papillae (i.e. special
} } structures endowed with epithelium and lamina propria located in the
} largest
} } forestomach of ruminants) in young cattle. The project is aimed at
} defining
} } the growth rate of these structures specialised in nutrients absorption,
} so
} } it is necessary to estimate the mitotic index. My questions are:
} }
} } 1.What staining would you prefer for seeing DNA and mitosis?
} } 2. Do you consider c-kit to be an adequate marker of the mitotic rate
} } through IHC in paraffin blocks?
} }
} } Thank you in advance
} }
} } Sincerely yours
} }
} } Agustin Jose Venzano Halliburton
} } DVM-Pathology Group
} } INTA, Argentina
}
} Geoff
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************
}
}
}
}
}


From daemon Tue Jun 4 08:15:00 2002



From: Awbrey, Donald :      DonaldAwbrey-at-texashealth.org
Date: Tue, 4 Jun 2002 08:07:21 -0500
Subject: TEM imaging plate technology

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Dear EM Netters,

Does anyoun out there have experience with imaging plate technology (i.e.
Ditabis)? I
would like to hear pros and cons concidering this technology. How does it
compare to
digital CCD camera systems (cost, time, supplies, reliability. We are
looking into possibly
retrofitting our JEOL 100CX with an off-line system.


Thanks in advance


Donald G. Awbrey, HT (ASCP), QIHC
Electron Microscopy / Image Analysis
817-878-5647
donaldawbrey-at-texashealth.org



From daemon Tue Jun 4 08:34:06 2002



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 5/31/02 2:33 PM
Subject: TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
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We upgraded an old Philips 300 with an AMT system with a Hamamatsu
C4742-95 camera 3.5 years ago. The system was mounted in the old 35mm
camera port, which does not have the resolution an under-the-column
camera will have, because I didn't want to lose the capability of
using film. The system has allowed a superior throughput of work with
same day turnarounds on projects that took 2 weeks before. Further,
digital image acquisition permits image analysis without any
intervening steps, which is crucial, for example, to calculating the
particle size of carbon black aggregates in a time-conscious
production environment.

For every day work, a laser printer works just fine. When printing
images for show/publication, an inkjet printer using a high quality
inkjet paper (Kodak or HP photo quality papers are my choice) does
very well. The ability to make high quality enlargements, though, is
restricted. Wet chemistry, film and paper is better, but not by
much...and, arguably, may not be worth the extra time and effort.

Not only does a digital system save on film, paper and chemistry (with
all the associated environmental concerns), it saves on
labor/time...and that is where the largest dollar savings is. Further,
as happened in my situation, I didn't need a darkroom any more so that
room was converted to house the new SEM/EDX system, which is a more
profitable use of the square footage. Even academic chairman (well,
in medical schools anyway) are concerned about the amount of research
dollars per square foot.

The choice of a system must always factor in service as well as
capability and price. AMT and Gatan both have excellent systems.
However, no one, given my circumstances at the time, would have chosen
an alternate system. Given another chance to buy a digital system for
a TEM, the AMT folks would have first shot, with the alternates having
an uphill, but not impossible, battle to convince me otherwise.

AMT competitors make excellent products. I'm just very satisfied with
cost, service, and instrumentation obtained from AMT. The usual
disclaimers apply.

Chuck Butterick
Degussa Corporation
Borger, TX


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We are looking to purchase a digital camera system for our TEM and
would like some feedback from those of you who already utilize such systems.
Some questions I'd like to ask are:

* What make & model camera system do you have?
* What type, make & model printer(s) do you use, (e.g. inkjet,
dye-sublimation, silver halide)?
* Do you feel you get micrograph-quality resolution and images
utilizing that system?
* Ease of use--is it user-friendly or cumbersome?
* Ease and/or quality of service--are any of the components
serviceable by you? How quickly & easily is it to get a service rep?
* How long have you had this system?
* Would you recommend this system--why or why not?
* What, if anything, might you have done differently?

I'd greatly appreciate any responses. Thank-you in advance for your
help.

Donna R. Clarkson





From daemon Tue Jun 4 08:58:10 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 04 Jun 2002 09:51:44 -0400
Subject: Re:TEM-Digital Camera Systems

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X-Mailer: QUALCOMM Windows Eudora Version 5.0.2


Alan is correct about the cost of film, etc. We charge our users 75¢US per
sheet for film to cover this cost. I disagree, though, about seeing the
image is in focus - if you have a digital system then you can use it to
check the image is in focus if you like, (and it certainly helps at very
high magnifications), but you can still record, with advantage, on film,
when the image will still be in focus.

The issue, though, goes beyond cost. For some users, digital imaging will
give excellent results, and meet all their needs. For others, the extreme
(by current standards) information density of conventional film is still
essential to get the job done. One has to be careful before deciding to
abandon an existing darkroom facility. Of course, the equation could be
different if a new facility is being designed. We still teach our users to
take all their images on film, for subsequent scanning. We do have digital
capture systems, but they are generally used only by outside users who
haven't learned how to handle film.

In changing from film to digital imaging, it is also important to
understand that significant re-education of users is important. The two
media have different characteristics, which must be understood and
accounted for if best results are to be obtained.

Tony.


At 01:34 PM 6/3/2002 -0500, you wrote:
} Bill
}
} What about the cost of film and chemicals over the lifetime of the
} microscope? As a one off charge your dept chair was happy but the
} additional costs of dealing with film are not small. Also as a totally
} digital materials science lab the main additional benefit is being able to
} see immediately the image and know it is in focus etc without wasting film
} and time.
}
} Alan


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Tue Jun 4 09:19:51 2002



From: Mike Marko :      marko-at-wadsworth.org
Date: Tue, 4 Jun 2002 10:04:48 -0400
Subject: TEM tomography workshop

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

A workshop on electron tomography will be held on 14-16 November 2002
in Albany, New York. The workshop is being offered at the Wadsworth
Center laboratories by the Resource for Visualization of Biological
Complexity (RVBC), a Biotechnology Resource supported by NCRR/NIH.

The workshop is intended to provide hands-on experience in electron
tomography, with an emphasis on data collection. Several different data
collection procedures, software packages, and electron microscopes will
be used. There will be daily practical sessions. There will also be
lectures, demonstrations, and practical sessions covering alignment and
reconstruction, visualization techniques, and preparation of
frozen-hydrated specimens.

Instructors and lecturers will include RVBC staff, as well the
individuals directly involved in software and technique development for
the systems that will be used during the workshop.

There is no registration fee.

Registration deadline: 1 October, 2002.

For more information and to register, please go to:

http://home.nycap.rr.com/cdmms/workshop

Michael Marko
Workshop Coordinator
Wadsworth Center, Albany, NY
marko-at-wadsworth.org


From daemon Tue Jun 4 09:29:48 2002



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Tue, 4 Jun 2002 10:28:38 -0400
Subject: Re: Legal requirements for microscope images

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Greetings Listers,

Normally I am a lurker, a parasite if you will, gleaning valuable
information and insights from the listings without contributing. However,
the current thread concerning the legality of "altered" or "enhanced"
digital images stirs old feelings from my youth in the days when I was a
forensic scientist (we called ourselves criminalists in those days). The
issue that was hot then between the attorneys, both prosecution and
defense, and myself was over the question of my resistance to introducing
any images at all to bolster my testimony as an expert witness. I think
that some of my arguments are still cogent and might be appropriate to
reconsider now.

First, if a putative expert witness survives voir dire and is declared an
expert by the court (and this must be done every time expert testimony is
to be presented, although it does get easier as one's reputation is
established), then the witness is permitted to render opinions which is
something an ordinary witness is not allowed to do. These opinions are
allowed based on the expectation that the expert possesses knowledge or
skills beyond those of the average person in the subject area(s) wherein
his or her testimony will be rendered. The expert's testimony is not and
should not be expected to inflame, excite, or even to educate the court or
the jurors to the point where they would be able to draw their own
conclusions from the same data set that the expert worked from. Hence, the
expert should not supply spectra, images, graphs, etc. to support his or
her testimony in open court.

Second, as has been alluded to in an earlier posting, questions concerning
the validity of testimony in the form of challenges from opposing expert
witnesses may well be expected and serve the valuable cause of keeping us
all honest. I never released raw or enhanced images or data to defense
experts, only samples of the specimens I had examined. It does not forward
the cause of justice to have one expert influence the opinion of another by
sharing the results of analyses or images. There is also no reason to
inflate the profits of independent consultants by supplying them with work
results done at the taxpayers expense.

Third, an image is only a representation of reality. It is not that
reality in and of itself. Therefore, even the "raw" image cannot be deemed
to convey any absolute information or truth about a sample or a scene in
the sense that it is entirely free of any alteration, subjectivity,
distortion, or misleading appearances. Further, if an individual is so
unscrupulous as to present false testimony, whether with supporting imagery
or not, then any supposed record of image enhancement would be highly
suspect as well.

} From the foregoing I would argue that there is no basis for a set of
"legal" restrictions on what can or cannot be done to an image. An image
is just an abstraction of reality. In many cases the image does allow us
to observe or interpret reality in a way that we as humans would be unable
to do given the limitations of our innate sensory abilities. However, we
will always have to deal with the further reality that the skill levels and
conscientiousness of all individuals who generate images are not equal,
just as not every referee will call the same pitch the same way, for
whatever reason. We are humans and rules don't make us better or worse
photographers.

Just some ramblings submitted for your consideration.



From daemon Tue Jun 4 09:39:46 2002



From: Clarkson Donna R Contr USAMRD :      donna.clarkson-at-brooks.af.mil
Date: 5/31/02 2:33 PM
Subject: TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
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Chuck,
Thank-you for your input. As with many others the opinions are mixed,
but seem to lean toward the AMT camera system. I am a little confused on one
issue, though. You mentioned that you have lost some resolution by
side-mounting the camera; others have noted that they thought they had lost
resolution by under-the-column mounting. Hmmmm, I guess it depends on the
scope it's mounted to.

Best regards,

Donna

Northrop Grumman Information Technology
for U S Army Medical Research Detachment
at Brooks Air Force Base
Phone (210) 536-1416
FAX (210) 536-1449
e-mail donna.clarkson-at-brooks.af.mil


-----Original Message-----
} From: Chuck Butterick [mailto:cbutte-at-ameripol.com]
Sent: Tuesday, June 04, 2002 8:15 AM
To: Microscopy-at-sparc5.microscopy.com; donna.clarkson-at-brooks.af.mil


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We are looking to purchase a digital camera system for our TEM and
would like some feedback from those of you who already utilize such systems.

Some questions I'd like to ask are:

* What make & model camera system do you have?
* What type, make & model printer(s) do you use, (e.g. inkjet,
dye-sublimation, silver halide)?
* Do you feel you get micrograph-quality resolution and images
utilizing that system?
* Ease of use--is it user-friendly or cumbersome?
* Ease and/or quality of service--are any of the components
serviceable by you? How quickly & easily is it to get a service rep?
* How long have you had this system?
* Would you recommend this system--why or why not?
* What, if anything, might you have done differently?

I'd greatly appreciate any responses. Thank-you in advance for your
help.

Donna R. Clarkson




From daemon Tue Jun 4 10:02:12 2002



From: Clarkson Donna R Contr USAMRD :      donna.clarkson-at-brooks.af.mil
Date: Tue, 4 Jun 2002 09:55:53 -0500
Subject: Ultracut Fluorescent bulbs

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Greg,
We have the Ultracut R and S, in addition to the E. If you need bulbs
for either of these you can order directly from Osram Sylvania at
www.osram.com. The R and S models use a U-shaped bulb, Sylvania Dulux S/E,
5W compact fluorescent, # 20315. I hope this helps.

Sincerely,
Donna R. Clarkson

Northrop Grumman Information Technology
for U S Army Medical Research Detachment
at Brooks Air Force Base
Phone (210) 536-1416
FAX (210) 536-1449
e-mail donna.clarkson-at-brooks.af.mil


-----Original Message-----
} From: Greg Strout [mailto:gstrout-at-ou.edu]
Sent: Monday, June 03, 2002 9:43 AM
To: Microscopy listserve


Does anyone know what the specs are for replacement fluorescent bulbs on
a Reichert Ultracut microtome? The specs are not in the manual and our
bulbs are so old that the printing is illegible. TIA.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Tue Jun 4 10:20:24 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 04 Jun 2002 10:13:39 -0500
Subject: Ge detector problem

Contents Retrieved from Microscopy Listserver Archives
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We have a Ge detector on our EDS system that has been there for several years.

Our normal procedure is to fill the dewar with LN2 twice a week (Monday or
Tuesday and then Thursday or Friday). We would like to think the dewar had
enough capacity to last a full week, and it used to. However, in the
end-of-the-week rush, I forgot to fill the dewar and came in to a warm
detector on Monday. It had been filled last Tuesday, so something seems to
be degrading that we no longer can go even 6 days on a fill.

We have an LN2 alarm, and it had worked and presumably cut off the high
voltage. We refilled the dewar, gave it an hour or more to cool off, then
tried the system again. We got a spectrum, but with artifacts.

I tried conditioning the detector (three times over 24 hours) and
recalibrating the electronics. The strobe peak got back into balance, but
artifacts still remain. I have posted three documents on the web at
ftp://www.marl.iastate.edu/Ge_detector/ for those that might be interested
in examining the problem for themselves. One file is a bitmap of a line
trace, another is a color bitmap, and the third, most detailed view, is a
figure embedded in a word document.

All three files show the same thing - an overlay of spectra from an
aluminum sample holder and a titanium standard. Both show the
characteristic peak but with perhaps more of a low energy tail. But the
real problem is a large hump in the background down-scale from the peak.
The hump follows the characteristic peak up and down the energy scale with
the element and always seems to peak at about 38% of the characteristic
energy.

I have been given one diagnosis and prognosis from the manufacturer. But I
wonder if the resident experts on this list might have a second opinion. I
welcome your comments, particularly if they might lead to a quicker,
cheaper fix than sending the detector back.

Warren

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Tue Jun 4 10:52:32 2002



From: Menon Sarath K Contr AFRL/MLLM :      Sarath.Menon-at-wpafb.af.mil
Date: Tue, 4 Jun 2002 11:42:14 -0400
Subject: Microprobe with FEG

Contents Retrieved from Microscopy Listserver Archives
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Could experts comment on advantages and disadvantages of using a microprobe with a FEG vs. a LaB6 emitter?
Pointers to any recent publications will be nice too.

thanks

Sarath Menon
Scientist
UES Inc.
Dayton, OH


From daemon Tue Jun 4 12:06:08 2002



From: Eric Anderson :      anderson_e-at-southernct.edu
Date: Tue, 04 Jun 2002 12:53:46 -0400
Subject: Re: Philips EM400 Matching Mains Transformer

Contents Retrieved from Microscopy Listserver Archives
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} Greetings All!
}
} We are in the process of setting up a second-hand EM400 acquired
} recently, and have found that the mains matching transformer was not
} included. Our raw supply main is 237 volts/60Hz (118V on one leg,
} 119V on the other, stability unknown), and I'm thinking this is not
} close enough to the specified 220V to go without the transformer. Any
} ideas? If we do need some line conditioning, can anyone recommend a
} particular device, or source for the original Philips transformer?
}
} Many thanks for any tips!
} -Eric
} --
}
Eric Anderson
SCSU Physics Adjunct
203-392-6455
anderson_e-at-southernct.edu

}



From daemon Tue Jun 4 13:10:47 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 4 Jun 2002 12:10:10 -0600
Subject: Re:TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tony,

you are of course right, that the "extreme" information density of film
cannot be approached today with digital cameras. This is where the different
types of digital TEM cameras come into play. On most microscopes there are
two possibilities to attach a camera: the 35 mm port (side-mount) and below
the film chamber (bottom-mount). BOTH types, by the way, allow you to keep
the film camera in most cases.

The side-mount cameras usually "see" an area that is approximately the same
as the area on a negative. Of course the resolution of the digital image is
then worse than the resolution on the film. The bottom-mounted cameras
usually "see" a much smaller area (plus there may be an addition
magnification due to geometric reasons), but they normally have a resolution
that is similar to film.

In our experience (and we have been producing these cameras for many years
now), there is almost never anybody who really needs the highest resolution
over a large area. In most cases the users fall into 2 groups: Users who
normally use the microscope to take images, then use the entire negative to
make a print. These are often biologists, who do not need sub-nm resolution.
In these cases the real issue is field of view and the appropriate camera
would be a side-mount camera.

On the other end are users who need the highest resolution (for example
materials scientists who do lattice imaging). Normally they need the highest
resolution in a very small area, though, and not over the entire field of a
negative. When I used to do this, it often turned out, that the sample was
slightly curved or other artifacts prohibited a good lattice imaging in
areas that were only nms away from the area I was looking at.

For example, if you take images at 500kx (for lattice imaging), the entire
field of view is roughly 200 nm. That is about 1000 lattice spacings in Si.
It is very unlikely, that you can keep the imaging conditions constant over
this distance to allow the use of the full negative.

And if you DO need higher resolution with a larger field of view, you can
always take several images with a slight overlap and montage them
electronically. In our software you can even do that automatically, provided
of course that you have a motorized stage. That way you can get images that
do approach the information density of film. However, the files tend to be
huge and can become a pain to work with.

Now, if we talk about information density, the amount of information per
pixel (or per unit area) has to be taken into account also. Typical CCDs
today are very linear and have 12- 14 bit information depth per pixel. That
corresponds to 4,000 to 16,000 levels of gray. Film, on the other hand has a
very non-linear characteristic, which makes it hard to get quantitative
information out of the film. As to the "bit -depth" of film: Film is really
a binary medium -- a grain can either be exposed or not. As far as I know,
there are no "partially exposed grains". If a grain in the film is about 5
microns in diameter, the film would have a 1-bit information depth on a 5
micron scale (on or off). On a 25 micron scale that would then roughly be a
5-bit resolution. In other words, a digital camera with 24 microns pixel
resolution has a much better bit-depth than film. Of course this is only a
crude approximation, as it does not take into account overlapping grains,
beam spread in the emulsion and other factors, so don't pin me on the
numbers.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Anthony J. Garratt-Reed [mailto:tonygr-at-mit.edu]
Sent: Tuesday, June 04, 2002 7:52 AM
To: Microscopy-at-sparc5.microscopy.com


Alan is correct about the cost of film, etc. We charge our users 75¢US per
sheet for film to cover this cost. I disagree, though, about seeing the
image is in focus - if you have a digital system then you can use it to
check the image is in focus if you like, (and it certainly helps at very
high magnifications), but you can still record, with advantage, on film,
when the image will still be in focus.

The issue, though, goes beyond cost. For some users, digital imaging will
give excellent results, and meet all their needs. For others, the extreme
(by current standards) information density of conventional film is still
essential to get the job done. One has to be careful before deciding to
abandon an existing darkroom facility. Of course, the equation could be
different if a new facility is being designed. We still teach our users to
take all their images on film, for subsequent scanning. We do have digital
capture systems, but they are generally used only by outside users who
haven't learned how to handle film.

In changing from film to digital imaging, it is also important to
understand that significant re-education of users is important. The two
media have different characteristics, which must be understood and
accounted for if best results are to be obtained.

Tony.


At 01:34 PM 6/3/2002 -0500, you wrote:
} Bill
}
} What about the cost of film and chemicals over the lifetime of the
} microscope? As a one off charge your dept chair was happy but the
} additional costs of dealing with film are not small. Also as a totally
} digital materials science lab the main additional benefit is being able to
} see immediately the image and know it is in focus etc without wasting film
} and time.
}
} Alan


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Tue Jun 4 14:00:20 2002



From: Steve Barlow :      sbarlow-at-sciences.sdsu.edu
Date: Tue, 4 Jun 2002 11:52:52 -0700
Subject: repair for old ISI sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers

My archeology trip to the storeroom uncovered a vintage ISI PS-2
sputter coater unit, which did not immediately start up when we
plugged it in and tried to pump it down. Does anyone know who might
service these babies, or have a Rosetta stone (i.e.,service manual
and schematics) for same?

thanks in advance

Steve
--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/


From daemon Tue Jun 4 15:06:13 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 04 Jun 2002 15:58:29 -0400
Subject: Re: Ge detector problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have seen a somewhat similar thing on a 12-year-old Si detector which I
know to have poor vacuum. The "hump" was more symmetric and much closer to
the parent peak (guessing, I would say at about 60-70% of the parent's
energy), and would appear particularly after I conditioned the detector,
reducing in height over a period of a day or two following the
conditioning. If it becomes useful, I'm sure I could dig out a sample
spectrum.

I have eliminated the effect by warming the detector (with hot water in the
dewar) and thoroughly pumping it (down to 10-8 Torr) before re-cooling. In
my case, this was easy because the detector is windowless, and is installed
on a UHV microscope (I had to bake the 'scope afterwards!). The full-width
at half-max resolution is still excellent, but I do get significant tailing
(i.e. degraded events), and am considering getting a new crystal, because I
assume I have crystal damage (or contamination?).

Tony.



} We have a Ge detector on our EDS system that has been there for several years.
}
} Our normal procedure is to fill the dewar with LN2 twice a week (Monday or
} Tuesday and then Thursday or Friday). We would like to think the dewar had
} enough capacity to last a full week, and it used to. However, in the
} end-of-the-week rush, I forgot to fill the dewar and came in to a warm
} detector on Monday. It had been filled last Tuesday, so something seems to
} be degrading that we no longer can go even 6 days on a fill.
}
} We have an LN2 alarm, and it had worked and presumably cut off the high
} voltage. We refilled the dewar, gave it an hour or more to cool off, then
} tried the system again. We got a spectrum, but with artifacts.
}
} I tried conditioning the detector (three times over 24 hours) and
} recalibrating the electronics. The strobe peak got back into balance, but
} artifacts still remain. I have posted three documents on the web at
} ftp://www.marl.iastate.edu/Ge_detector/ for those that might be interested
} in examining the problem for themselves. One file is a bitmap of a line
} trace, another is a color bitmap, and the third, most detailed view, is a
} figure embedded in a word document.
}
} All three files show the same thing - an overlay of spectra from an
} aluminum sample holder and a titanium standard. Both show the
} characteristic peak but with perhaps more of a low energy tail. But the
} real problem is a large hump in the background down-scale from the peak.
} The hump follows the characteristic peak up and down the energy scale with
} the element and always seems to peak at about 38% of the characteristic energy.
}
} I have been given one diagnosis and prognosis from the manufacturer. But I
} wonder if the resident experts on this list might have a second opinion. I
} welcome your comments, particularly if they might lead to a quicker,
} cheaper fix than sending the detector back.
}
} Warren
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 23 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking
}
}


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Tue Jun 4 15:25:08 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 04 Jun 2002 13:35:40 -0700
Subject: Re: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Donald

Before I bought the digital camera, I was looking on that image plate
technology. It seems to me, it does not have serious advantage for most of
us. I would like to concentrate my findings in a few sentences.

Image plate PRO:
- has more pixels that most digital cameras (some top-end cameras
HAS similar amount).
- has dynamic range same as digital cameras 12-16 bit.
- it's more sensitive than film.
- very good for electron diffraction experiments!!! (only one real plus to me).
- manufacturers claimed that pixel resolution on the plate is comparable
with film (it's difficult to proof because you have to scan film to
compare, so it'll depend from the film scanner).

Image plate CONTRA:
Imitate the film procedure - you have to perform all procedures as for
film, load/upload plates into cassettes (in the dark), change the magazine
(wait for vacuum), load plates into the scanner (in the dark I believe),
wait for scanning - 2 (or more, don't remember, up to 5 at full resolution
I believe) min etc. So, it does not eliminate the dark-room, scanning is
slow and then you have to process/save/print data. Time consuming. You
have all disadvantages the classical film use: plate may be scratched
during loading/uploading, deformed, lost, dropped on floor with valuable
image etc. One plate is $100 I believe. No practical use in most
biological applications I believe. I am not warranty that all my
information is current, I was shopping for image plate system a few years
ago and my comments represent the situation of that time. Sorry,
manufacturing gays. Sergey

At 08:07 AM 6/4/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Tue Jun 4 15:48:29 2002



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Tue, 4 Jun 2002 15:41:15 -0500
Subject: RE: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sergey et al,

I'd just like to point out that instead of relying on uncertain
recollections, there is some good work in the literature which discusses
both slow-scan CCD's and imaging plates. Here's a start:

J. M. Zuo "Electron detection characteristics of slow-scan CCD camera"
Ultramicroscopy 66 (1996) 21-33.

J. M. Zuo, M. R. McCartney and J. C. H. Spence "Performance of imaging
plates for electron recording" Ultramicroscopy 66 (1996) 35-47

G. Y. Fan and M. H. Ellisman "Digital imaging in transmission electron
microscopy" Journal of Microscopy 200 (2000) 1-13.

Although the technology is continuously evolving, the basic points are still
valid.

Regards,
Wharton

*************************************************
Wharton Sinkler, Ph.D.
UOP LLC
25 E. Algonquin Rd.
Des Plaines, IL 60017-5017
847-391-3878


} -----Original Message-----
} From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} Sent: Tuesday, June 04, 2002 3:36 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Re: TEM imaging plate technology
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Donald
}
} Before I bought the digital camera, I was looking on that image plate
} technology. It seems to me, it does not have serious advantage for most
} of
} us. I would like to concentrate my findings in a few sentences.
}
} Image plate PRO:
} - has more pixels that most digital cameras (some top-end cameras
} HAS similar amount).
} - has dynamic range same as digital cameras 12-16 bit.
} - it's more sensitive than film.
} - very good for electron diffraction experiments!!! (only one real plus to
} me).
} - manufacturers claimed that pixel resolution on the plate is comparable
} with film (it's difficult to proof because you have to scan film to
} compare, so it'll depend from the film scanner).
}
} Image plate CONTRA:
} Imitate the film procedure - you have to perform all procedures as for
} film, load/upload plates into cassettes (in the dark), change the magazine
}
} (wait for vacuum), load plates into the scanner (in the dark I believe),
} wait for scanning - 2 (or more, don't remember, up to 5 at full resolution
}
} I believe) min etc. So, it does not eliminate the dark-room, scanning is
} slow and then you have to process/save/print data. Time consuming. You
} have all disadvantages the classical film use: plate may be scratched
} during loading/uploading, deformed, lost, dropped on floor with valuable
} image etc. One plate is $100 I believe. No practical use in most
} biological applications I believe. I am not warranty that all my
} information is current, I was shopping for image plate system a few years
} ago and my comments represent the situation of that time. Sorry,
} manufacturing gays. Sergey
}
} At 08:07 AM 6/4/02 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} }
} } Dear EM Netters,
} }
} } Does anyoun out there have experience with imaging plate technology (i.e.
} } Ditabis)? I
} } would like to hear pros and cons concidering this technology. How does
} it
} } compare to
} } digital CCD camera systems (cost, time, supplies, reliability. We are
} } looking into possibly
} } retrofitting our JEOL 100CX with an off-line system.
} }
} }
} } Thanks in advance
} }
} }
} } Donald G. Awbrey, HT (ASCP), QIHC
} } Electron Microscopy / Image Analysis
} } 817-878-5647
} } donaldawbrey-at-texashealth.org
} }
}
} ------------------------------------------------------
}
} Sergey Ryazantsev, Ph.D.
} Electron Microscopy
} Department of Biological Chemistry, School of Medicine
} University of California, Los Angeles
} Box 951737
} Los Angeles, CA 90095-1737
}
} (310) 825-1144 (office)
} Pager: (310) 845-0248
} FAX: (310) 206-5272 (departmental)
} mailto:sryazant-at-ucla.edu
}


From daemon Tue Jun 4 18:38:21 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 4 Jun 2002 19:35:19 -0400
Subject: Microprobe with FEG

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sarath;

I'm sure you will get many opinions on this question but I'll offer mine
anyway even though there are plenty of folks out there that have more
specific knowledge and may provide empirical comparisons and some literature
references. However, I have both a LaB6 and a FESEM side by side, both with
identical Si/sapphire SUTW windows, light element detectors, going into a
common processor from the same vendor. In my opinion, and some considerable
experience in this comparison, unless doing very low voltage work such as
high resolution elemental mapping of light elements,e.g. C, O, F, N, which
is more easily done with a FEG, the physics of electron-solid interactions
essentially dictate the results. My biggest source of error [spatially] is
beam drift when doing elemental mapping and that is influenced, in my
experience, much more by mechanical vibration, sample charging, stage
moving, heavy footed people near the column etc., than beam stability, beam
shape, astigmatism etc.

In any event, a coulomb of charge, is a coulomb of charge, no matter what
gun it originates from. The physics of the electron-solid interaction
doesn't care which gun the electron was pulled out of. But for high mag.,
high res. imaging, I'll take the FEG all the time. The nice thing about
running the lab., AND working in it, is you get to tell everyone else they
have to use the "other" SEM.

Peter Tomic
Failure Analysis & Analytical Services
Anadigics, Inc.

-----Original Message-----
} From: Menon Sarath K Contr AFRL/MLLM [mailto:Sarath.Menon-at-wpafb.af.mil]
Sent: Tuesday, June 04, 2002 11:42 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Could experts comment on advantages and disadvantages of using a microprobe
with a FEG vs. a LaB6 emitter?
Pointers to any recent publications will be nice too.

thanks

Sarath Menon
Scientist
UES Inc.
Dayton, OH


From daemon Tue Jun 4 19:17:24 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 4 Jun 2002 20:16:01 -0400
Subject: Re: Philips EM400 Matching Mains Transformer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric;

I'm actually surprised that the two legs are within a volt! You must have a
better power company than we do. If you put a strip-chart recorder on your
A.C. line, you will see all kinds of fluctuations over time, especially
during high demand periods in the summer months. In any event, your
instrument should have a voltage regulator within it and a step-down
transformer, often to 100 volts. I wouldn't spend the money on a line
regulator or U.P.S. unless you find some issues with the instrument since
its' power supply should be capable of "cleaning" up a noisy line . Unless
the instrument was designed by a high school student in Wood Shop, the D.C.
supply should be well regulated with little ripple or A.C. component in its'
D.C. supply and generally immune to most A.C. line fluctuations.

We lost power yesterday for 10 seconds and a turbomolecular pump on an FIB
rotated it's last rotation. Now we're talking real misery.

Peter

-----Original Message-----
} From: Eric Anderson [mailto:anderson_e-at-southernct.edu]
Sent: Tuesday, June 04, 2002 12:54 PM
To: Microscopy-at-sparc5.microscopy.com


} Greetings All!
}
} We are in the process of setting up a second-hand EM400 acquired
} recently, and have found that the mains matching transformer was not
} included. Our raw supply main is 237 volts/60Hz (118V on one leg,
} 119V on the other, stability unknown), and I'm thinking this is not
} close enough to the specified 220V to go without the transformer. Any
} ideas? If we do need some line conditioning, can anyone recommend a
} particular device, or source for the original Philips transformer?
}
} Many thanks for any tips!
} -Eric
} --
}
Eric Anderson
SCSU Physics Adjunct
203-392-6455
anderson_e-at-southernct.edu

}



From daemon Tue Jun 4 19:48:30 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 04 Jun 2002 17:41:40 -0700
Subject: RE: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Wharton

Are you seriously think, people will read that very specific papers? Do
you think, so many of us is ready to go through all this math etc? I agree,
it's very important to understand the scientific foundation for device you
are going to use. But, in practice you have deal not with such nice things
as PSF (point spread function) or MTF. The camera with very nice PSF may
have terrible design and not suitable for use. Company, who manufactured
that baby may not respond on your telephone calls and finally you may
figure out that it does not exists anymore. I do read scientific journals
and it's extremely easy to find informations on the Internet nova days,
ListServer to me is a place where we have chance to share our personal
experience (bad or good), which mostly is not published and not indexed by
Medline/CC whatever. Anyway, thank you for the reference, I enjoyed
reading it. Sergey

At 01:41 PM 6/4/02, you wrote:

} Sergey et al,
}
} I'd just like to point out that instead of relying on uncertain
} recollections, there is some good work in the literature which discusses
} both slow-scan CCD's and imaging plates. Here's a start:
}
} J. M. Zuo "Electron detection characteristics of slow-scan CCD camera"
} Ultramicroscopy 66 (1996) 21-33.
}
} J. M. Zuo, M. R. McCartney and J. C. H. Spence "Performance of imaging
} plates for electron recording" Ultramicroscopy 66 (1996) 35-47
}
} G. Y. Fan and M. H. Ellisman "Digital imaging in transmission electron
} microscopy" Journal of Microscopy 200 (2000) 1-13.
}
} Although the technology is continuously evolving, the basic points are still
} valid.
}
} Regards,
} Wharton
}
} *************************************************
} Wharton Sinkler, Ph.D.
} UOP LLC
} 25 E. Algonquin Rd.
} Des Plaines, IL 60017-5017
} 847-391-3878
}
}
} } -----Original Message-----
} } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } Sent: Tuesday, June 04, 2002 3:36 PM
} } To: microscopy-at-sparc5.microscopy.com
} } Subject: Re: TEM imaging plate technology
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Donald
} }
} } Before I bought the digital camera, I was looking on that image plate
} } technology. It seems to me, it does not have serious advantage for most
} } of
} } us. I would like to concentrate my findings in a few sentences.
} }
} } Image plate PRO:
} } - has more pixels that most digital cameras (some top-end cameras
} } HAS similar amount).
} } - has dynamic range same as digital cameras 12-16 bit.
} } - it's more sensitive than film.
} } - very good for electron diffraction experiments!!! (only one real plus to
} } me).
} } - manufacturers claimed that pixel resolution on the plate is comparable
} } with film (it's difficult to proof because you have to scan film to
} } compare, so it'll depend from the film scanner).
} }
} } Image plate CONTRA:
} } Imitate the film procedure - you have to perform all procedures as for
} } film, load/upload plates into cassettes (in the dark), change the magazine
} }
} } (wait for vacuum), load plates into the scanner (in the dark I believe),
} } wait for scanning - 2 (or more, don't remember, up to 5 at full resolution
} }
} } I believe) min etc. So, it does not eliminate the dark-room, scanning is
} } slow and then you have to process/save/print data. Time consuming. You
} } have all disadvantages the classical film use: plate may be scratched
} } during loading/uploading, deformed, lost, dropped on floor with valuable
} } image etc. One plate is $100 I believe. No practical use in most
} } biological applications I believe. I am not warranty that all my
} } information is current, I was shopping for image plate system a few years
} } ago and my comments represent the situation of that time. Sorry,
} } manufacturing gays. Sergey
} }
} } At 08:07 AM 6/4/02 -0500, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } }
} } } Dear EM Netters,
} } }
} } } Does anyoun out there have experience with imaging plate technology (i.e.
} } } Ditabis)? I
} } } would like to hear pros and cons concidering this technology. How does
} } it
} } } compare to
} } } digital CCD camera systems (cost, time, supplies, reliability. We are
} } } looking into possibly
} } } retrofitting our JEOL 100CX with an off-line system.
} } }
} } }
} } } Thanks in advance
} } }
} } }
} } } Donald G. Awbrey, HT (ASCP), QIHC
} } } Electron Microscopy / Image Analysis
} } } 817-878-5647
} } } donaldawbrey-at-texashealth.org
} } }
} }
} } ------------------------------------------------------
} }
} } Sergey Ryazantsev, Ph.D.
} } Electron Microscopy
} } Department of Biological Chemistry, School of Medicine
} } University of California, Los Angeles
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } (310) 825-1144 (office)
} } Pager: (310) 845-0248
} } FAX: (310) 206-5272 (departmental)
} } mailto:sryazant-at-ucla.edu
} }

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Jun 5 00:30:18 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Wed, 05 Jun 2002 05:53:47 -0400
Subject: Re: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric,

237V is a bit too high. It must be between 208V and 220V. The simplest and
economical way to power up your TEM is to use Buck and Boost transformer
connected as autotransformer. Philips matching unit originally supplied with
EM400, was also an autotransformer. Order from Grainger, www.grainger.com ,
many locations everywhere, stock # 1H270, $174. This one will reduce your
line voltage by 24V, making it 213V to 211V (see below), which is perfect.

Notes.
1) Make sure that grounding is correct and safe.
2)This unit can be connected in 4 different ways, for increasing or reducing
the line voltage by 12V or 24V, and is shipped with 1 page manual. Read it.
3) The above part number is given for your particular (line voltage) case.
Others may require a transformer with different part number.
4) Transformer will not stabilize the line voltage, only change it.
5) I assume that you measured line voltage with no load connected- the
actual voltage may drop about 1V or 2V when you connect the TEM.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Eric Anderson {anderson_e-at-southernct.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, June 04, 2002 12:53 PM


The Ditabis plates now offer with resolution up to 6000x5000 and 20 bit
data for FAR less money than a lower resolution CCD solution. The newest
system even offers variable resolution. The higher sensitivity is often
useful for low dose images as well. There are applications where one or the
other may be the right choice but certainly biological image should not be
excluded. You failed to mention any of the downside for CCD devices - of
which there is, of course, a comparable list that includes limited image
area and dynamic range, image resolution and cost.

Bill Miller
ElectroImage


At 01:35 PM 6/4/2002 -0700, Sergey Ryazantsev wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jun 5 05:49:10 2002



From: Khalid Boulahya :      khalid-at-quim.ucm.es
Date: Wed, 05 Jun 2002 12:42:40 +0200
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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**************************************************************
Khalid Boulahya
Depto de Química Inorgánica
Facultad De Químicas
Universidad Complutense de Madrid (UCM)
28040 Spain
khalid-at-quim.ucm.es
**************************************************************



From daemon Wed Jun 5 05:50:23 2002



From: Khalid Boulahya :      khalid-at-quim.ucm.es
Date: Wed, 05 Jun 2002 12:45:04 +0200
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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subscribe

**************************************************************
Khalid Boulahya
Depto de Química Inorgánica
Facultad De Químicas
Universidad Complutense de Madrid (UCM)
28040 Spain
khalid-at-quim.ucm.es
**************************************************************



From daemon Wed Jun 5 06:31:42 2002



From: Khalid Boulahya :      khalid-at-quim.ucm.es
Date: Wed, 05 Jun 2002 13:24:48 +0200
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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subscribe



From daemon Wed Jun 5 07:19:06 2002



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Wed, 05 Jun 2002 14:10:51 +0200
Subject: Re: TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
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Dear Donna,

} From the camera resolution point of view, in principle, there is no difference
between the side-mounted and bottom-mounted position. Of course the
electron-optical resolution has to be adapted accordingly – in order to acquire
the wished field of view. For example, the mag must be increased by of factor of
3x if a side-mounted cameras is used. This is not easy possible for high
resolution work, therefore mainly bottom-mounted cameras are used for such
applications.

But there is an essential difference between both positions: The used aperture
of the bottom mounted camera is much smaller, this means the image is captured
very close to the optical axis. Therefore these images will show almost no
distortions which are due to the aberrations of the projective lenses.
At the side-mounted position these aberrations can be up to 1-2% which seems not
to be very much. But for a 1k camera, this means a displacement of 10-20 pixels
which makes problems when images are stitched together.
In practise, if you consider to combine images together (stitching, tiling) - in
order to get a higher resolution or larger field of view - you should consider a
bottom-mounted camera.

I hope this helps to clarify the situation.

Best wishes,
Ingo

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
We have moved. Please notice our new address.

Dr. Ingo Daberkow
Tietz Video and Image Processing Systems GmbH
Eremitenweg 1
D-82131 Gauting, Germany
Tel: +49-89-8506567
FAX: +49-89-8509488
Internet: http://www.tvips.com/
Email: ingo.daberkow-at-tvips.com
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++



Clarkson Donna R Contr USAMRD wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Chuck,
} Thank-you for your input. As with many others the opinions are mixed,
} but seem to lean toward the AMT camera system. I am a little confused on one
} issue, though. You mentioned that you have lost some resolution by
} side-mounting the camera; others have noted that they thought they had lost
} resolution by under-the-column mounting. Hmmmm, I guess it depends on the
} scope it's mounted to.
}
} Best regards,
}
} Donna
}
} Northrop Grumman Information Technology
} for U S Army Medical Research Detachment
} at Brooks Air Force Base
} Phone (210) 536-1416
} FAX (210) 536-1449
} e-mail donna.clarkson-at-brooks.af.mil
}
} -----Original Message-----
} } From: Chuck Butterick [mailto:cbutte-at-ameripol.com]
} Sent: Tuesday, June 04, 2002 8:15 AM
} To: Microscopy-at-sparc5.microscopy.com; donna.clarkson-at-brooks.af.mil
} Subject: Re: TEM-Digital Camera Systems
}
} We upgraded an old Philips 300 with an AMT system with a Hamamatsu
} C4742-95 camera 3.5 years ago. The system was mounted in the old 35mm
} camera port, which does not have the resolution an under-the-column
} camera will have, because I didn't want to lose the capability of
} using film. The system has allowed a superior throughput of work with
} same day turnarounds on projects that took 2 weeks before. Further,
} digital image acquisition permits image analysis without any
} intervening steps, which is crucial, for example, to calculating the
} particle size of carbon black aggregates in a time-conscious
} production environment.
}
} For every day work, a laser printer works just fine. When printing
} images for show/publication, an inkjet printer using a high quality
} inkjet paper (Kodak or HP photo quality papers are my choice) does
} very well. The ability to make high quality enlargements, though, is
} restricted. Wet chemistry, film and paper is better, but not by
} much...and, arguably, may not be worth the extra time and effort.
}
} Not only does a digital system save on film, paper and chemistry (with
} all the associated environmental concerns), it saves on
} labor/time...and that is where the largest dollar savings is. Further,
} as happened in my situation, I didn't need a darkroom any more so that
} room was converted to house the new SEM/EDX system, which is a more
} profitable use of the square footage. Even academic chairman (well,
} in medical schools anyway) are concerned about the amount of research
} dollars per square foot.
}
} The choice of a system must always factor in service as well as
} capability and price. AMT and Gatan both have excellent systems.
} However, no one, given my circumstances at the time, would have chosen
} an alternate system. Given another chance to buy a digital system for
} a TEM, the AMT folks would have first shot, with the alternates having
} an uphill, but not impossible, battle to convince me otherwise.
}
} AMT competitors make excellent products. I'm just very satisfied with
} cost, service, and instrumentation obtained from AMT. The usual
} disclaimers apply.
}
} Chuck Butterick
} Degussa Corporation
} Borger, TX
}
} ______________________________ Reply Separator
} _________________________________
} Subject: TEM-Digital Camera Systems
} Author: Clarkson Donna R Contr USAMRD {donna.clarkson-at-brooks.af.mil} at
} INTERNET-MAIL
} Date: 5/31/02 2:33 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We are looking to purchase a digital camera system for our TEM and
} would like some feedback from those of you who already utilize such systems.
}
} Some questions I'd like to ask are:
}
} * What make & model camera system do you have?
} * What type, make & model printer(s) do you use, (e.g. inkjet,
} dye-sublimation, silver halide)?
} * Do you feel you get micrograph-quality resolution and images
} utilizing that system?
} * Ease of use--is it user-friendly or cumbersome?
} * Ease and/or quality of service--are any of the components
} serviceable by you? How quickly & easily is it to get a service rep?
} * How long have you had this system?
} * Would you recommend this system--why or why not?
} * What, if anything, might you have done differently?
}
} I'd greatly appreciate any responses. Thank-you in advance for your
} help.
}
} Donna R. Clarkson
}
}



From daemon Wed Jun 5 07:51:30 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 05 Jun 2002 08:43:53 -0400
Subject: Re: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
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I was just dealing with the company that will be the US rep. for Ditabis. They are ElectroImage at www.electroimage.com
As far as I can tell, the plates are not light sensitive until after they have been exposed and each plate is useful for up to 1000 exposures (you just "erase" the previous image). Iwas told that the unit with all the bells and whistles will cost around $50 to $60K. I think it might be a good thing for people who want digital, but I never saw it in action.

We still are old fashioned and want negatives that we can archive.

Paula :-)

p.s. I have no financial interest in ElectroImage I just know that they are going to sell the beasty.

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

} } } "Awbrey, Donald" {DonaldAwbrey-at-texashealth.org} 06/04/02 09:07AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America




Dear EM Netters,

Does anyoun out there have experience with imaging plate technology (i.e.
Ditabis)? I
would like to hear pros and cons concidering this technology. How does it
compare to
digital CCD camera systems (cost, time, supplies, reliability. We are
looking into possibly
retrofitting our JEOL 100CX with an off-line system.


Thanks in advance


Donald G. Awbrey, HT (ASCP), QIHC
Electron Microscopy / Image Analysis
817-878-5647
donaldawbrey-at-texashealth.org





From daemon Wed Jun 5 08:31:08 2002



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Wed, 5 Jun 2002 08:23:15 -0500
Subject: RE: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
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OK, Truce Sergey!

Your point is well taken, and yes, design considerations and convenience may
overwhelm a good MTF or linearity.

However, I think you are mistaken about dynamic range being similar for
Imaging Plates and CCD's. That being said, I have gone back to look at the
papers I mentioned and I'm not able to find a clear statement of what I
mean.

I believe (maybe someone will set us straight on this) that the saturation
of the IP is considerably higher than the CCD. By 'saturation' I mean the
dose (electrons/unit area) at which there is significant deviation from
linearity.

The reason saturation level is important (and why the IP is great for
diffraction) is that signal/noise at low electron doses is dominated by shot
noise. So if you are trying to record a diffraction pattern with a CCD with
a single exposure without saturating the CCD at the strong spots, the weak
reflections will be noisy simply because relatively few electrons are
arriving there (statistical or 'shot' noise). With a higher saturation (I
believe the case for IP), you can expose longer and get better statistics
overall. (Of course you can also record multiple exposures with the CCD at
different times and merge the data).

Regards,
Wharton



} -----Original Message-----
} From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} Sent: Tuesday, June 04, 2002 7:42 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: TEM imaging plate technology
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Wharton
}
} Are you seriously think, people will read that very specific papers? Do
} you think, so many of us is ready to go through all this math etc? I
} agree,
} it's very important to understand the scientific foundation for device you
}
} are going to use. But, in practice you have deal not with such nice
} things
} as PSF (point spread function) or MTF. The camera with very nice PSF may
} have terrible design and not suitable for use. Company, who manufactured
} that baby may not respond on your telephone calls and finally you may
} figure out that it does not exists anymore. I do read scientific
} journals
} and it's extremely easy to find informations on the Internet nova days,
} ListServer to me is a place where we have chance to share our personal
} experience (bad or good), which mostly is not published and not indexed by
}
} Medline/CC whatever. Anyway, thank you for the reference, I enjoyed
} reading it. Sergey
}
} At 01:41 PM 6/4/02, you wrote:
}
} } Sergey et al,
} }
} } I'd just like to point out that instead of relying on uncertain
} } recollections, there is some good work in the literature which discusses
} } both slow-scan CCD's and imaging plates. Here's a start:
} }
} } J. M. Zuo "Electron detection characteristics of slow-scan CCD camera"
} } Ultramicroscopy 66 (1996) 21-33.
} }
} } J. M. Zuo, M. R. McCartney and J. C. H. Spence "Performance of imaging
} } plates for electron recording" Ultramicroscopy 66 (1996) 35-47
} }
} } G. Y. Fan and M. H. Ellisman "Digital imaging in transmission electron
} } microscopy" Journal of Microscopy 200 (2000) 1-13.
} }
} } Although the technology is continuously evolving, the basic points are
} still
} } valid.
} }
} } Regards,
} } Wharton
} }
} } *************************************************
} } Wharton Sinkler, Ph.D.
} } UOP LLC
} } 25 E. Algonquin Rd.
} } Des Plaines, IL 60017-5017
} } 847-391-3878
} }
} }
} } } -----Original Message-----
} } } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } } Sent: Tuesday, June 04, 2002 3:36 PM
} } } To: microscopy-at-sparc5.microscopy.com
} } } Subject: Re: TEM imaging plate technology
} } }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Donald
} } }
} } } Before I bought the digital camera, I was looking on that image plate
} } } technology. It seems to me, it does not have serious advantage for
} most
} } } of
} } } us. I would like to concentrate my findings in a few sentences.
} } }
} } } Image plate PRO:
} } } - has more pixels that most digital cameras (some top-end cameras
} } } HAS similar amount).
} } } - has dynamic range same as digital cameras 12-16 bit.
} } } - it's more sensitive than film.
} } } - very good for electron diffraction experiments!!! (only one real
} plus to
} } } me).
} } } - manufacturers claimed that pixel resolution on the plate is
} comparable
} } } with film (it's difficult to proof because you have to scan film to
} } } compare, so it'll depend from the film scanner).
} } }
} } } Image plate CONTRA:
} } } Imitate the film procedure - you have to perform all procedures as for
} } } film, load/upload plates into cassettes (in the dark), change the
} magazine
} } }
} } } (wait for vacuum), load plates into the scanner (in the dark I
} believe),
} } } wait for scanning - 2 (or more, don't remember, up to 5 at full
} resolution
} } }
} } } I believe) min etc. So, it does not eliminate the dark-room, scanning
} is
} } } slow and then you have to process/save/print data. Time consuming.
} You
} } } have all disadvantages the classical film use: plate may be scratched
} } } during loading/uploading, deformed, lost, dropped on floor with
} valuable
} } } image etc. One plate is $100 I believe. No practical use in most
} } } biological applications I believe. I am not warranty that all my
} } } information is current, I was shopping for image plate system a few
} years
} } } ago and my comments represent the situation of that time. Sorry,
} } } manufacturing gays. Sergey
} } }
} } } At 08:07 AM 6/4/02 -0500, you wrote:
} } }
} } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } -----------------------------------------------------------------------.
} } } }
} } } }
} } } }
} } } }
} } } } Dear EM Netters,
} } } }
} } } } Does anyoun out there have experience with imaging plate technology
} (i.e.
} } } } Ditabis)? I
} } } } would like to hear pros and cons concidering this technology. How
} does
} } } it
} } } } compare to
} } } } digital CCD camera systems (cost, time, supplies, reliability. We
} are
} } } } looking into possibly
} } } } retrofitting our JEOL 100CX with an off-line system.
} } } }
} } } }
} } } } Thanks in advance
} } } }
} } } }
} } } } Donald G. Awbrey, HT (ASCP), QIHC
} } } } Electron Microscopy / Image Analysis
} } } } 817-878-5647
} } } } donaldawbrey-at-texashealth.org
} } } }
} } }
} } } ------------------------------------------------------
} } }
} } } Sergey Ryazantsev, Ph.D.
} } } Electron Microscopy
} } } Department of Biological Chemistry, School of Medicine
} } } University of California, Los Angeles
} } } Box 951737
} } } Los Angeles, CA 90095-1737
} } }
} } } (310) 825-1144 (office)
} } } Pager: (310) 845-0248
} } } FAX: (310) 206-5272 (departmental)
} } } mailto:sryazant-at-ucla.edu
} } }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}


From daemon Wed Jun 5 08:31:31 2002



From: Clarkson Donna R Contr USAMRD :      donna.clarkson-at-brooks.af.mil
Date: Wed, 5 Jun 2002 08:25:49 -0500
Subject: Re:TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey,
Don't worry about sounding like an advertisement. The more information
I can gather on the digital cameras the better. I did find it rather amusing
to read your "disclaimer" at the end stating that you were not bribed with
dinner or coffee! Good sense of humor! Thanks.

Donna R. Clarkson




-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Monday, June 03, 2002 11:02 PM
To: Microscopy-at-sparc5.microscopy.com


Using digital camera (600W BioScan, Gatan) with TEM over last couple of
month I find the following.

-most people prefer to use digital imaging even we offer equal access to
the film as well. It's because in most cases people need to evaluate
sample quality etc, they simply don't need film quality. From another hand
they could use film anytime if they wanted.

-with digital images you have chance to see your sample immediately
(important for evaluation/students in rush/deadlines etc).

-If you do not enlarge the digital image, 7x7" draft printout on laser
printer (standard) is looks very similar to the image produced from scanned
film. Manipulating with image resolution, dpi etc, you should keep in
mind, that 1200 dpi printer resolution mean that printer could produce 1200
dots per inch in the row making solid line. In order to create shadows of
gray, printer should print dots with space. For instance, 50% of grey
would be represented by 'dot-space' sequence. It mean, that resolution
would be 50% from 1200 = 600 dpi. For 30% gray, it would be 400 dpi. So,
there is no reason to abuse printer sending 1200 dpi 50 Mb image. On
practice, I could not recognize difference between 1x1K at 144 dpi digital
camera image from scanned from film 1200 dpi image: On laserJet (similarly
on our Tektronix dye-sub) - they are looks very the same. BUT: the
difference is that you could enlarge 1200 dpi image (and print with the
same quality) and you COULDN'T do so with 1x1K image from digital
camera. Another remark here: in 'prestigious' magazines like
Science/Nature technical editors have tendency to reduce image to the
postal stamp size, so you could do it by yourself and there is no need to
use film for such small images, digital camera will work just fine!

-another advantage of the digital camera, which, actually, I did not
expect, is its sensitivity. My camera is at least x10 more sensitive than
film, so exposure time is 0.1-0.2 sec versus my usual 1.5-2 sec for the
film, So, less drift and sample damage.

-another things are educational. Instead keep students in the dark and
teach them how to adjust binoculars (instead doing actual EM), we are
comfortably sitting around the 19" flat screen monitor with lights ON (and
nice, not loud classic music). I find it's more convenient to show how to
focus image on the computer screen rather than make a 'focus series', then
develop/print it. At this point students usually don't remember how image
was looks like on the microscope's screen and we have to start again. I
don't mean that students don't need to know how to operate
microscope/binoculars, I just mean that digital camera makes this process
more enjoyable and creative.

-another thing is academic: The live image from digital camera I broadcast
to the Departmental network, so you could see live image on any
departmental computer. PIs now comfortably sit in their offices and enjoy
watching how their students working on the TEM. Similarly it works for
Internet. We did a few session when our collaborators observe their
samples sitting far-far away from UCLA.

So, the bottom line is that TEM digital camera is very convenient
'supplement' to your EM. It does not replace the film, but enhance your
microscope's abilities. It's like power-steering in the car: convenient,
but not necessary - you may park your car in NY without this 'supplement'
right? As a matter of fact, cars without power-steering is cheaper.

Sergey

P.S. I just figured out that this message may be recognized as an
'advertisement'. So, it looks like I was working hard writing on ESL for
manufacturers. I would like to declare openly - I did not intend to do so
and nobody from Gatan or other manufacturer offered to me dinner or coffee
when I was writing it. I did it in sincere believe of what I was writing.








From daemon Wed Jun 5 09:24:32 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Wed, 05 Jun 2002 09:51:07 -0400
Subject: Temporary Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello there, the University of Michigan North Campus EMAL has an opening
available for a temporary Research Associate II Engineering. The generic
job description for a Research Associate II Engineering may be found on the
U of M web site (http://www.umich.edu/%7Ehraa/erc/descriptions/cd12871.htm)
The specific requirements for this position are as follows:

Research Assoc. II Engineering
Operate, assist and train users in the use of the following NC EMAL lab
instruments: JEOL 2010 FEG Analytical Transmission Electron Microscope, JEOL
4000EX High Resolution Transmission Electron Microscope, Philips XL30 FEG
SEM Scanning Electron Microscope, ElectroScan E3 Environmental Scanning
Electron Microscope, Digital Instruments Scanning Force Microscope, PHI 5400
X-ray Photo-electron Spectrometer, PHI 660 Scanning Auger Microprobe,
Darkroom facilities, specimen preparation equipment. Assist users with
reduction and analysis of data recorded on same instruments.

Maintenance of some lab equipment including PHI 5400 XPS, PHI 660 Auger,
Environmental SEM, darkroom and specimen preparation equipment. Prepare
internal and external billing for EMAL instrument use each month. Purchase
lab supplies. Take part in collaborative projects with other members of the
university. Develop personal research projects as time permits. Other duties
as defined by supervisor.

The position is full time, but will be short-term only ( less than 11
months)
This position lies in the University Professional/Administrative salary
grade 9 which currently extends from $11.25 per hour to $29.45 per hour
maximum. The actual compensation will depend on the candidate experience.

Minimum qualifications are a bachelors degree in science or engineering and
several years experience in the field of electron microscopy and surface
analysis. Ideal qualifications are a bachelors degree in materials science,
physics, chemistry, engineering or a closely related subject and 5 years of
experience in electron microscopy and surface analysis or a masters degree
in a related subject and 3 years experience in electron microscopy and
surface analysis. Computer literacy is required on two of the following
platforms: PC, Mac, Unix.

Please respond by email or phone to John Mansfield, information in my
signature below.


--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
USA
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"



From daemon Wed Jun 5 09:42:29 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Wed, 5 Jun 2002 10:17:44 -0400
Subject: Jeol 100C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a JEOL 100C top entry scope that is looking for a new home
for anyone interested. It has 2 sets of plate film boxes and holders.
Asking $10,000 or BO with removal and shipping at labor and cost. Scope
has been under service contract with Jeol USA for the last 10 years.


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432
jgoodhouse-at-molbio.princeton.edu



From daemon Wed Jun 5 10:03:45 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Wed, 5 Jun 2002 10:55:29 -0700
Subject: heating lead citrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,
What safety precautions should be followed when heating lead citrate?
I want to try Hanaichi et al's method - A Stable Lead by Modification of
Sato's Method.(1986) J. Electron Microsc. Vol. 35 No. 3, 304-306.
It calls for heating lead citrate for several hours in a melting pot at 200
- 300 C. I have access to a muffle furnace (it's in a hood). Are there
other safety precautions we should use?
thanks in advance for the advice,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Wed Jun 5 10:14:17 2002



From: Mike Marko :      marko-at-wadsworth.org
Date: Wed, 5 Jun 2002 10:59:20 -0400
Subject: TEM - Tomography Workshop

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

A workshop on electron tomography will be held on 14-16 November 2002
in Albany, New York. The workshop is being offered at the Wadsworth
Center laboratories by the Resource for Visualization of Biological
Complexity (RVBC), a Biotechnology Resource supported by NCRR/NIH.

The workshop is intended to provide hands-on experience in electron
tomography, with an emphasis on data collection. Several different data
collection procedures, software packages, and electron microscopes will
be used. There will be daily practical sessions. There will also be
lectures, demonstrations, and practical sessions covering alignment and
reconstruction, visualization techniques, and preparation of
frozen-hydrated specimens.

Instructors and lecturers will include RVBC staff, as well the
individuals directly involved in software and technique development for
the systems that will be used during the workshop.

There is no registration fee.

Registration deadline: 1 October, 2002.

For more information and to register, please go to:

http://home.nycap.rr.com/cdmms/workshop

Michael Marko
Workshop Coordinator
Wadsworth Center, Albany, NY
marko-at-wadsworth.org


From daemon Wed Jun 5 10:31:06 2002



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Wed, 5 Jun 2002 11:25:58 -0400
Subject: RE: repair for old ISI sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I also have an ISI PS-2 coater. When I was looking for documentation, I was
told (by a person who provides maintenance for EM equipment) that the
Polaron E5000 was pretty much the same unit. I got an E5000 manual and the
control descriptions match what I see on my unit, so it seems to be a good
match. I have not opened the unit to compare the schematic against the
actual circuitry, though.

Hope that helps.

Bruce Girrell



From daemon Wed Jun 5 10:31:23 2002



From: Eric Anderson :      anderson_e-at-southernct.edu
Date: Wed, 05 Jun 2002 11:25:22 -0400
Subject: Re: Philips EM400 Matching Mains Transformer

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all for your replies and generous consideration! I think we have
this one problem licked, but our troubleshooting has only just begun. Well,
back to work!

Best regards,
-Eric
---------------------------
Vitaly Feingold wrote:

} Eric,
}
} 237V is a bit too high. It must be between 208V and 220V. The simplest and
} economical way to power up your TEM is to use Buck and Boost transformer
} connected as autotransformer. Philips matching unit originally supplied with
} EM400, was also an autotransformer. Order from Grainger, www.grainger.com ,
} many locations everywhere, stock # 1H270, $174. This one will reduce your
} line voltage by 24V, making it 213V to 211V (see below), which is perfect.
}
} Notes.
} 1) Make sure that grounding is correct and safe.
} 2)This unit can be connected in 4 different ways, for increasing or reducing
} the line voltage by 12V or 24V, and is shipped with 1 page manual. Read it.
} 3) The above part number is given for your particular (line voltage) case.
} Others may require a transformer with different part number.
} 4) Transformer will not stabilize the line voltage, only change it.
} 5) I assume that you measured line voltage with no load connected- the
} actual voltage may drop about 1V or 2V when you connect the TEM.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
} ----- Original Message -----
} From: Eric Anderson {anderson_e-at-southernct.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, June 04, 2002 12:53 PM
} Subject: Re: Philips EM400 Matching Mains Transformer
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } } Greetings All!
} } }
} } } We are in the process of setting up a second-hand EM400 acquired
} } } recently, and have found that the mains matching transformer was not
} } } included. Our raw supply main is 237 volts/60Hz (118V on one leg,
} } } 119V on the other, stability unknown), and I'm thinking this is not
} } } close enough to the specified 220V to go without the transformer. Any
} } } ideas? If we do need some line conditioning, can anyone recommend a
} } } particular device, or source for the original Philips transformer?
} } }
} } } Many thanks for any tips!
} } } -Eric
} } } --
} } }
} } Eric Anderson
} } SCSU Physics Adjunct
} } 203-392-6455
} } anderson_e-at-southernct.edu



From daemon Wed Jun 5 11:48:13 2002



From: Stephenson, Matthew :      stephenson-at-impactanalytical.com
Date: Wed, 5 Jun 2002 12:39:41 -0400
Subject: AFM calibration - standards and procedures

Contents Retrieved from Microscopy Listserver Archives
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Greetings all!

I'm in the process of resuscitating a TopoMetrix TMX 2000 with Discoverer
stage for contact and non-contact AFM. We use three scanners: a 70 micron
tripod, a 7 micron tube, and a 0.76 micron tube. The instrument is up and
running, so the time has come to calibrate, incorporate into our quality
program, and get some customers. My questions are as follows:

1. What reference standards are recommended for x, y, and z distance
calibration? Is anything available that is NIST traceable, or barring that,
certifiable by a reputable authority?

2. Have widely accepted AFM calibration procedures emerged in the
microscopy community? I'd be particularly interested in citations for sound
calibration procedure, and hoary wisdom regarding the effect of instrument
variables on the calibration process and accuracy.

I look forward to your comments!

Matthew K. Stephenson
Analytical Associate
Impact Analytical
1910 West Saint Andrews Road
Midland, MI 48640
(989) 832-5555 X506
stephenson-at-impactanalytical.com




From daemon Wed Jun 5 13:38:14 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 5 Jun 2002 12:32:26 -0600
Subject: RE: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Careful: the following is an opinion of a CCD system manufacturer. Reader
discretion is advised!

If I remember correctly, the manufacturers for he imaging plates claim a
dynamic range of 20 bit, which is considerably more than the 12 or 14 bit
from a CCD camera. If that, however, makes a big difference for diffraction
patterns I don't know. If you want to record the weakest spots, you need to
expose at least for a time so that the signal is above noise. Since CCD
cameras are very sensitive (down to single electron sensitivity), there is
not much that can be improved by the imaging plates. There are no half
electrons (and if you see 1/3 electrons, book a flight to Stockholm). If the
20 bits are sufficient to image the weakest beams together with the
transmitted beam without saturation I don't know. With the Imaging plates
you can probably get the weakest spots and more medium intensity spots at
the same time than with a CCD. With a CCD you have the advantage that you
immediately see the result and can adjust your exposure accordingly. It's
also very easy to take exposures at different exposure times and combine
them, as the CCDs are very linear.

I can't remember anything about linearity of the imaging plates. But the way
they work (excite crystals into a semi-stable state through the light from a
phosphor and then "read' them by relaxing into ground stage through a
laser), I would think there are some non-linearities as with increasing
exposure you have less and less crystallites that can be excited. Whether
that's critical for quantitative measurements I can't say.

Finally, the images on the plates are stable for a few hours. So you can't
leave the plates in for a few days if you want the best results.

One area, where in my experience (as a TEM user) CCD cameras have a big
advantage is dark field imaging. I remember taking several films with
different exposures to make sure that I had one that was right. With a CCD
there is no guesswork anymore, as you can see the images immediately.

Resolution: theoretically the plate film should have a worse resolution that
film. The electron beam has to travel through the crystallite layer, create
light in the phosphor layer, and the photons then have to travel back to the
crystallites to excite them. This should add to the dispersion of the beam,
resulting in a reduced resolution. The manufacturers of the imaging plates
claim around 20 microns resolution. If that is a 20 micron resolution at 20
bit dynamic range they don't say.

Again, we produce those CCD systems, and I definitely have a bias. But I
thought I put my 2 cents in.

Mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Wednesday, June 05, 2002 7:23 AM
To: 'Sergey Ryazantsev'; Microscopy-at-sparc5.microscopy.com



OK, Truce Sergey!

Your point is well taken, and yes, design considerations and convenience may
overwhelm a good MTF or linearity.

However, I think you are mistaken about dynamic range being similar for
Imaging Plates and CCD's. That being said, I have gone back to look at the
papers I mentioned and I'm not able to find a clear statement of what I
mean.

I believe (maybe someone will set us straight on this) that the saturation
of the IP is considerably higher than the CCD. By 'saturation' I mean the
dose (electrons/unit area) at which there is significant deviation from
linearity.

The reason saturation level is important (and why the IP is great for
diffraction) is that signal/noise at low electron doses is dominated by shot
noise. So if you are trying to record a diffraction pattern with a CCD with
a single exposure without saturating the CCD at the strong spots, the weak
reflections will be noisy simply because relatively few electrons are
arriving there (statistical or 'shot' noise). With a higher saturation (I
believe the case for IP), you can expose longer and get better statistics
overall. (Of course you can also record multiple exposures with the CCD at
different times and merge the data).

Regards,
Wharton



} -----Original Message-----
} From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} Sent: Tuesday, June 04, 2002 7:42 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: TEM imaging plate technology
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Wharton
}
} Are you seriously think, people will read that very specific papers? Do
} you think, so many of us is ready to go through all this math etc? I
} agree,
} it's very important to understand the scientific foundation for device you
}
} are going to use. But, in practice you have deal not with such nice
} things
} as PSF (point spread function) or MTF. The camera with very nice PSF may
} have terrible design and not suitable for use. Company, who manufactured
} that baby may not respond on your telephone calls and finally you may
} figure out that it does not exists anymore. I do read scientific
} journals
} and it's extremely easy to find informations on the Internet nova days,
} ListServer to me is a place where we have chance to share our personal
} experience (bad or good), which mostly is not published and not indexed by
}
} Medline/CC whatever. Anyway, thank you for the reference, I enjoyed
} reading it. Sergey
}
} At 01:41 PM 6/4/02, you wrote:
}
} } Sergey et al,
} }
} } I'd just like to point out that instead of relying on uncertain
} } recollections, there is some good work in the literature which discusses
} } both slow-scan CCD's and imaging plates. Here's a start:
} }
} } J. M. Zuo "Electron detection characteristics of slow-scan CCD camera"
} } Ultramicroscopy 66 (1996) 21-33.
} }
} } J. M. Zuo, M. R. McCartney and J. C. H. Spence "Performance of imaging
} } plates for electron recording" Ultramicroscopy 66 (1996) 35-47
} }
} } G. Y. Fan and M. H. Ellisman "Digital imaging in transmission electron
} } microscopy" Journal of Microscopy 200 (2000) 1-13.
} }
} } Although the technology is continuously evolving, the basic points are
} still
} } valid.
} }
} } Regards,
} } Wharton
} }
} } *************************************************
} } Wharton Sinkler, Ph.D.
} } UOP LLC
} } 25 E. Algonquin Rd.
} } Des Plaines, IL 60017-5017
} } 847-391-3878
} }
} }
} } } -----Original Message-----
} } } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } } Sent: Tuesday, June 04, 2002 3:36 PM
} } } To: microscopy-at-sparc5.microscopy.com
} } } Subject: Re: TEM imaging plate technology
} } }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Donald
} } }
} } } Before I bought the digital camera, I was looking on that image plate
} } } technology. It seems to me, it does not have serious advantage for
} most
} } } of
} } } us. I would like to concentrate my findings in a few sentences.
} } }
} } } Image plate PRO:
} } } - has more pixels that most digital cameras (some top-end cameras
} } } HAS similar amount).
} } } - has dynamic range same as digital cameras 12-16 bit.
} } } - it's more sensitive than film.
} } } - very good for electron diffraction experiments!!! (only one real
} plus to
} } } me).
} } } - manufacturers claimed that pixel resolution on the plate is
} comparable
} } } with film (it's difficult to proof because you have to scan film to
} } } compare, so it'll depend from the film scanner).
} } }
} } } Image plate CONTRA:
} } } Imitate the film procedure - you have to perform all procedures as for
} } } film, load/upload plates into cassettes (in the dark), change the
} magazine
} } }
} } } (wait for vacuum), load plates into the scanner (in the dark I
} believe),
} } } wait for scanning - 2 (or more, don't remember, up to 5 at full
} resolution
} } }
} } } I believe) min etc. So, it does not eliminate the dark-room, scanning
} is
} } } slow and then you have to process/save/print data. Time consuming.
} You
} } } have all disadvantages the classical film use: plate may be scratched
} } } during loading/uploading, deformed, lost, dropped on floor with
} valuable
} } } image etc. One plate is $100 I believe. No practical use in most
} } } biological applications I believe. I am not warranty that all my
} } } information is current, I was shopping for image plate system a few
} years
} } } ago and my comments represent the situation of that time. Sorry,
} } } manufacturing gays. Sergey
} } }
} } } At 08:07 AM 6/4/02 -0500, you wrote:
} } }
} } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } -----------------------------------------------------------------------.
} } } }
} } } }
} } } }
} } } }
} } } } Dear EM Netters,
} } } }
} } } } Does anyoun out there have experience with imaging plate technology
} (i.e.
} } } } Ditabis)? I
} } } } would like to hear pros and cons concidering this technology. How
} does
} } } it
} } } } compare to
} } } } digital CCD camera systems (cost, time, supplies, reliability. We
} are
} } } } looking into possibly
} } } } retrofitting our JEOL 100CX with an off-line system.
} } } }
} } } }
} } } } Thanks in advance
} } } }
} } } }
} } } } Donald G. Awbrey, HT (ASCP), QIHC
} } } } Electron Microscopy / Image Analysis
} } } } 817-878-5647
} } } } donaldawbrey-at-texashealth.org
} } } }
} } }
} } } ------------------------------------------------------
} } }
} } } Sergey Ryazantsev, Ph.D.
} } } Electron Microscopy
} } } Department of Biological Chemistry, School of Medicine
} } } University of California, Los Angeles
} } } Box 951737
} } } Los Angeles, CA 90095-1737
} } }
} } } (310) 825-1144 (office)
} } } Pager: (310) 845-0248
} } } FAX: (310) 206-5272 (departmental)
} } } mailto:sryazant-at-ucla.edu
} } }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}


From daemon Wed Jun 5 14:01:35 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 05 Jun 2002 12:12:31 -0700
Subject: virus alert

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I just got message with subject "Subject: To call in the service
representative." from microscopy-at-sparc5.microscopy.com with attachment
aa_160x100[1].exe

This message contains some virus. Unfortunately my Norton Antivirus
eliminate it so quickly and I have no chance to find what virus it was.
Thanks for such nice gift. May be it's sort of sign, I am talking too much
on ListServer? Sergey
------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Wed Jun 5 15:22:08 2002



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Wed, 05 Jun 2002 22:13:44 +0200
Subject: Re: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sergey,

Just another remark regarding the dynamic range. The imaging plates have a much
higher dynamic range than other electron detectors (up to 20 bit after several
scans). But please notice the dynamic range is defined as: (maximum signal/RMS
noise) – this is not the digitization level of the analog-to-digital converter.
This extreme high dynamic range is only necessary for the acquisition of
diffraction patterns. A “normal” bright-field image has typically a dynamic
range of about 100, which is mainly limited by the noise of the electrons
(shot-noise). This means, for such applications CCD cameras have the advantage
of a real-time acquisition (WYSIWYG) and furthermore the camera can be used for
checking or controlling of the TEM parameters (defocus, astigmatism, etc.)

Personally I have found the literature list of Wharton very useful – which
enables the interesting reader to collect more specific information.

Best wishes,
Ingo

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Dr. Ingo Daberkow
Tietz Video and Image Processing Systems GmbH
Eremitenweg 1
D-82131 Gauting, Germany
Tel: +49-89-8506567
FAX: +49-89-8509488
Internet: http://www.tvips.com/
Email: ingo.daberkow-at-tvips.com
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

,



"Sinkler, Wharton" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} OK, Truce Sergey!
}
} Your point is well taken, and yes, design considerations and convenience may
} overwhelm a good MTF or linearity.
}
} However, I think you are mistaken about dynamic range being similar for
} Imaging Plates and CCD's. That being said, I have gone back to look at the
} papers I mentioned and I'm not able to find a clear statement of what I
} mean.
}
} I believe (maybe someone will set us straight on this) that the saturation
} of the IP is considerably higher than the CCD. By 'saturation' I mean the
} dose (electrons/unit area) at which there is significant deviation from
} linearity.
}
} The reason saturation level is important (and why the IP is great for
} diffraction) is that signal/noise at low electron doses is dominated by shot
} noise. So if you are trying to record a diffraction pattern with a CCD with
} a single exposure without saturating the CCD at the strong spots, the weak
} reflections will be noisy simply because relatively few electrons are
} arriving there (statistical or 'shot' noise). With a higher saturation (I
} believe the case for IP), you can expose longer and get better statistics
} overall. (Of course you can also record multiple exposures with the CCD at
} different times and merge the data).
}
} Regards,
} Wharton
}
} } -----Original Message-----
} } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } Sent: Tuesday, June 04, 2002 7:42 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: RE: TEM imaging plate technology
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Wharton
} }
} } Are you seriously think, people will read that very specific papers? Do
} } you think, so many of us is ready to go through all this math etc? I
} } agree,
} } it's very important to understand the scientific foundation for device you
} }
} } are going to use. But, in practice you have deal not with such nice
} } things
} } as PSF (point spread function) or MTF. The camera with very nice PSF may
} } have terrible design and not suitable for use. Company, who manufactured
} } that baby may not respond on your telephone calls and finally you may
} } figure out that it does not exists anymore. I do read scientific
} } journals
} } and it's extremely easy to find informations on the Internet nova days,
} } ListServer to me is a place where we have chance to share our personal
} } experience (bad or good), which mostly is not published and not indexed by
} }
} } Medline/CC whatever. Anyway, thank you for the reference, I enjoyed
} } reading it. Sergey
} }
} } At 01:41 PM 6/4/02, you wrote:
} }
} } } Sergey et al,
} } }
} } } I'd just like to point out that instead of relying on uncertain
} } } recollections, there is some good work in the literature which discusses
} } } both slow-scan CCD's and imaging plates. Here's a start:
} } }
} } } J. M. Zuo "Electron detection characteristics of slow-scan CCD camera"
} } } Ultramicroscopy 66 (1996) 21-33.
} } }
} } } J. M. Zuo, M. R. McCartney and J. C. H. Spence "Performance of imaging
} } } plates for electron recording" Ultramicroscopy 66 (1996) 35-47
} } }
} } } G. Y. Fan and M. H. Ellisman "Digital imaging in transmission electron
} } } microscopy" Journal of Microscopy 200 (2000) 1-13.
} } }
} } } Although the technology is continuously evolving, the basic points are
} } still
} } } valid.
} } }
} } } Regards,
} } } Wharton
} } }
} } } *************************************************
} } } Wharton Sinkler, Ph.D.
} } } UOP LLC
} } } 25 E. Algonquin Rd.
} } } Des Plaines, IL 60017-5017
} } } 847-391-3878
} } }
} } }
} } } } -----Original Message-----
} } } } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } } } Sent: Tuesday, June 04, 2002 3:36 PM
} } } } To: microscopy-at-sparc5.microscopy.com
} } } } Subject: Re: TEM imaging plate technology
} } } }
} } } }
} } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Dear Donald
} } } }
} } } } Before I bought the digital camera, I was looking on that image plate
} } } } technology. It seems to me, it does not have serious advantage for
} } most
} } } } of
} } } } us. I would like to concentrate my findings in a few sentences.
} } } }
} } } } Image plate PRO:
} } } } - has more pixels that most digital cameras (some top-end cameras
} } } } HAS similar amount).
} } } } - has dynamic range same as digital cameras 12-16 bit.
} } } } - it's more sensitive than film.
} } } } - very good for electron diffraction experiments!!! (only one real
} } plus to
} } } } me).
} } } } - manufacturers claimed that pixel resolution on the plate is
} } comparable
} } } } with film (it's difficult to proof because you have to scan film to
} } } } compare, so it'll depend from the film scanner).
} } } }
} } } } Image plate CONTRA:
} } } } Imitate the film procedure - you have to perform all procedures as for
} } } } film, load/upload plates into cassettes (in the dark), change the
} } magazine
} } } }
} } } } (wait for vacuum), load plates into the scanner (in the dark I
} } believe),
} } } } wait for scanning - 2 (or more, don't remember, up to 5 at full
} } resolution
} } } }
} } } } I believe) min etc. So, it does not eliminate the dark-room, scanning
} } is
} } } } slow and then you have to process/save/print data. Time consuming.
} } You
} } } } have all disadvantages the classical film use: plate may be scratched
} } } } during loading/uploading, deformed, lost, dropped on floor with
} } valuable
} } } } image etc. One plate is $100 I believe. No practical use in most
} } } } biological applications I believe. I am not warranty that all my
} } } } information is current, I was shopping for image plate system a few
} } years
} } } } ago and my comments represent the situation of that time. Sorry,
} } } } manufacturing gays. Sergey
} } } }
} } } } At 08:07 AM 6/4/02 -0500, you wrote:
} } } }
} } } ------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } } -----------------------------------------------------------------------.
} } } } }
} } } } }
} } } } }
} } } } }
} } } } } Dear EM Netters,
} } } } }
} } } } } Does anyoun out there have experience with imaging plate technology
} } (i.e.
} } } } } Ditabis)? I
} } } } } would like to hear pros and cons concidering this technology. How
} } does
} } } } it
} } } } } compare to
} } } } } digital CCD camera systems (cost, time, supplies, reliability. We
} } are
} } } } } looking into possibly
} } } } } retrofitting our JEOL 100CX with an off-line system.
} } } } }
} } } } }
} } } } } Thanks in advance
} } } } }
} } } } }
} } } } } Donald G. Awbrey, HT (ASCP), QIHC
} } } } } Electron Microscopy / Image Analysis
} } } } } 817-878-5647
} } } } } donaldawbrey-at-texashealth.org
} } } } }
} } } }
} } } } ------------------------------------------------------
} } } }
} } } } Sergey Ryazantsev, Ph.D.
} } } } Electron Microscopy
} } } } Department of Biological Chemistry, School of Medicine
} } } } University of California, Los Angeles
} } } } Box 951737
} } } } Los Angeles, CA 90095-1737
} } } }
} } } } (310) 825-1144 (office)
} } } } Pager: (310) 845-0248
} } } } FAX: (310) 206-5272 (departmental)
} } } } mailto:sryazant-at-ucla.edu
} } } }
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }



From daemon Wed Jun 5 15:38:10 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 05 Jun 2002 15:31:51 -0500
Subject: Re: virus alert

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Did the message come with the standard MSA header? I doubt it, but it
should if it really came via the list. (Right, Nestor?) I have not received
the message you described.

I have had a number of virus reports sent back to me that a message of mine
had a virus that was caught by one of the growing number of corporate virus
checkers. My name was listed as the alleged sender of the message; however,
the return path listed a different account. I remember reading something on
the McAfee site that such is the behavior of several of the viruses out there.

So, some virus probably is spoofing the MSA address. Nestor has done a good
job of stopping most of the trash from getting through.

Warren

At 12:12 PM 6/5/02 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Wed Jun 5 16:00:45 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 05 Jun 2002 13:54:23 -0700
Subject: Re: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ingo
Thanks for comments. I absolutely agree with folks who mentioned extremely
wide dynamic range for image plates. I think there is some misunderstood
happening: In order to get data from image plate, you have to scan it and
record it as a digital image. The quality of scanners is different, some
of them could not provide 20 bit gray scale. When I pointed, that dynamic
range is the same for the images from CCD and IP I mean that the images
were recorded in 12-16 bit format, which is very usual for nova days. 20
bit format is non-readable by most software and therefore useless for such
simple person like me, who is still using Photoshop for most jobs. So,
from this point of view, we have practically the same dynamic range of the
final digital image. I am sorry if my posting confuse somebody. Image
plate technology widely used in X-ray crystallography and as a substitution
for X-ray film in molecular biology applications for decades. No question
about that: this is a great technology. BUT, my point, is that current
realization of this technology in EM does not have serious advantages over
normal film/digital camera combination. AND: keep in mind, how long you
will scan to get those 20 bits dynamic range... Thanks again for the very
good point. Sergey


At 01:13 PM 6/5/02, you wrote:
} Dear Sergey,
}
} Just another remark regarding the dynamic range. The imaging plates have a
} much
} higher dynamic range than other electron detectors (up to 20 bit after several
} scans). But please notice the dynamic range is defined as: (maximum signal/RMS
} noise) ­ this is not the digitization level of the analog-to-digital
} converter.
} This extreme high dynamic range is only necessary for the acquisition of
} diffraction patterns. A “normal” bright-field image has typically a dynamic
} range of about 100, which is mainly limited by the noise of the electrons
} (shot-noise). This means, for such applications CCD cameras have the advantage
} of a real-time acquisition (WYSIWYG) and furthermore the camera can be
} used for
} checking or controlling of the TEM parameters (defocus, astigmatism, etc.)
}
} Personally I have found the literature list of Wharton very useful ­ which
} enables the interesting reader to collect more specific information.
}
} Best wishes,
} Ingo
}
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} Dr. Ingo Daberkow
} Tietz Video and Image Processing Systems GmbH
} Eremitenweg 1
} D-82131 Gauting, Germany
} Tel: +49-89-8506567
} FAX: +49-89-8509488
} Internet: http://www.tvips.com/
} Email: ingo.daberkow-at-tvips.com
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
} ,
}
}
}
} "Sinkler, Wharton" wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } OK, Truce Sergey!
} }
} } Your point is well taken, and yes, design considerations and
} convenience may
} } overwhelm a good MTF or linearity.
} }
} } However, I think you are mistaken about dynamic range being similar for
} } Imaging Plates and CCD's. That being said, I have gone back to look at the
} } papers I mentioned and I'm not able to find a clear statement of what I
} } mean.
} }
} } I believe (maybe someone will set us straight on this) that the saturation
} } of the IP is considerably higher than the CCD. By 'saturation' I mean the
} } dose (electrons/unit area) at which there is significant deviation from
} } linearity.
} }
} } The reason saturation level is important (and why the IP is great for
} } diffraction) is that signal/noise at low electron doses is dominated by
} shot
} } noise. So if you are trying to record a diffraction pattern with a CCD
} with
} } a single exposure without saturating the CCD at the strong spots, the weak
} } reflections will be noisy simply because relatively few electrons are
} } arriving there (statistical or 'shot' noise). With a higher saturation (I
} } believe the case for IP), you can expose longer and get better statistics
} } overall. (Of course you can also record multiple exposures with the CCD at
} } different times and merge the data).
} }
} } Regards,
} } Wharton
} }
} } } -----Original Message-----
} } } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } } Sent: Tuesday, June 04, 2002 7:42 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: RE: TEM imaging plate technology
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Wharton
} } }
} } } Are you seriously think, people will read that very specific papers? Do
} } } you think, so many of us is ready to go through all this math etc? I
} } } agree,
} } } it's very important to understand the scientific foundation for
} device you
} } }
} } } are going to use. But, in practice you have deal not with such nice
} } } things
} } } as PSF (point spread function) or MTF. The camera with very nice PSF may
} } } have terrible design and not suitable for use. Company, who manufactured
} } } that baby may not respond on your telephone calls and finally you may
} } } figure out that it does not exists anymore. I do read scientific
} } } journals
} } } and it's extremely easy to find informations on the Internet nova days,
} } } ListServer to me is a place where we have chance to share our personal
} } } experience (bad or good), which mostly is not published and not
} indexed by
} } }
} } } Medline/CC whatever. Anyway, thank you for the reference, I enjoyed
} } } reading it. Sergey
} } }
} } } At 01:41 PM 6/4/02, you wrote:
} } }
} } } } Sergey et al,
} } } }
} } } } I'd just like to point out that instead of relying on uncertain
} } } } recollections, there is some good work in the literature which discusses
} } } } both slow-scan CCD's and imaging plates. Here's a start:
} } } }
} } } } J. M. Zuo "Electron detection characteristics of slow-scan CCD camera"
} } } } Ultramicroscopy 66 (1996) 21-33.
} } } }
} } } } J. M. Zuo, M. R. McCartney and J. C. H. Spence "Performance of imaging
} } } } plates for electron recording" Ultramicroscopy 66 (1996) 35-47
} } } }
} } } } G. Y. Fan and M. H. Ellisman "Digital imaging in transmission electron
} } } } microscopy" Journal of Microscopy 200 (2000) 1-13.
} } } }
} } } } Although the technology is continuously evolving, the basic points are
} } } still
} } } } valid.
} } } }
} } } } Regards,
} } } } Wharton
} } } }
} } } } *************************************************
} } } } Wharton Sinkler, Ph.D.
} } } } UOP LLC
} } } } 25 E. Algonquin Rd.
} } } } Des Plaines, IL 60017-5017
} } } } 847-391-3878
} } } }
} } } }
} } } } } -----Original Message-----
} } } } } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } } } } Sent: Tuesday, June 04, 2002 3:36 PM
} } } } } To: microscopy-at-sparc5.microscopy.com
} } } } } Subject: Re: TEM imaging plate technology
} } } } }
} } } } }
} } } ------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} } } -----------------------------------------------------------------------.
} } } } }
} } } } }
} } } } } Dear Donald
} } } } }
} } } } } Before I bought the digital camera, I was looking on that image plate
} } } } } technology. It seems to me, it does not have serious advantage for
} } } most
} } } } } of
} } } } } us. I would like to concentrate my findings in a few sentences.
} } } } }
} } } } } Image plate PRO:
} } } } } - has more pixels that most digital cameras (some top-end cameras
} } } } } HAS similar amount).
} } } } } - has dynamic range same as digital cameras 12-16 bit.
} } } } } - it's more sensitive than film.
} } } } } - very good for electron diffraction experiments!!! (only one real
} } } plus to
} } } } } me).
} } } } } - manufacturers claimed that pixel resolution on the plate is
} } } comparable
} } } } } with film (it's difficult to proof because you have to scan film to
} } } } } compare, so it'll depend from the film scanner).
} } } } }
} } } } } Image plate CONTRA:
} } } } } Imitate the film procedure - you have to perform all procedures
} as for
} } } } } film, load/upload plates into cassettes (in the dark), change the
} } } magazine
} } } } }
} } } } } (wait for vacuum), load plates into the scanner (in the dark I
} } } believe),
} } } } } wait for scanning - 2 (or more, don't remember, up to 5 at full
} } } resolution
} } } } }
} } } } } I believe) min etc. So, it does not eliminate the dark-room,
} scanning
} } } is
} } } } } slow and then you have to process/save/print data. Time consuming.
} } } You
} } } } } have all disadvantages the classical film use: plate may be scratched
} } } } } during loading/uploading, deformed, lost, dropped on floor with
} } } valuable
} } } } } image etc. One plate is $100 I believe. No practical use in most
} } } } } biological applications I believe. I am not warranty that all my
} } } } } information is current, I was shopping for image plate system a few
} } } years
} } } } } ago and my comments represent the situation of that time. Sorry,
} } } } } manufacturing gays. Sergey
} } } } }
} } } } } At 08:07 AM 6/4/02 -0500, you wrote:
} } } } }
} } } } ------------------------------------------------------------------------
} } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America
} } } } } } To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com
} } } } } } On-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} } } } -----------------------------------------------------------------------.
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } } Dear EM Netters,
} } } } } }
} } } } } } Does anyoun out there have experience with imaging plate technology
} } } (i.e.
} } } } } } Ditabis)? I
} } } } } } would like to hear pros and cons concidering this technology. How
} } } does
} } } } } it
} } } } } } compare to
} } } } } } digital CCD camera systems (cost, time, supplies, reliability. We
} } } are
} } } } } } looking into possibly
} } } } } } retrofitting our JEOL 100CX with an off-line system.
} } } } } }
} } } } } }
} } } } } } Thanks in advance
} } } } } }
} } } } } }
} } } } } } Donald G. Awbrey, HT (ASCP), QIHC
} } } } } } Electron Microscopy / Image Analysis
} } } } } } 817-878-5647
} } } } } } donaldawbrey-at-texashealth.org
} } } } } }
} } } } }
} } } } } ------------------------------------------------------
} } } } }
} } } } } Sergey Ryazantsev, Ph.D.
} } } } } Electron Microscopy
} } } } } Department of Biological Chemistry, School of Medicine
} } } } } University of California, Los Angeles
} } } } } Box 951737
} } } } } Los Angeles, CA 90095-1737
} } } } }
} } } } } (310) 825-1144 (office)
} } } } } Pager: (310) 845-0248
} } } } } FAX: (310) 206-5272 (departmental)
} } } } } mailto:sryazant-at-ucla.edu
} } } } }
} } }
} } } _____________________________________
} } }
} } } Sergey Ryazantsev Ph. D.
} } } Electron Microscopy
} } } UCLA School of Medicine
} } } Department of Biological Chemistry
} } } Box 951737
} } } Los Angeles, CA 90095-1737
} } }
} } } Phone: (310) 825-1144
} } } FAX (departmental): (310) 206-5272
} } } mailto:sryazant-at-ucla.edu
} } }
} } }
} } }

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Jun 5 16:03:48 2002



From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Wed, 05 Jun 2002 14:02:42 -0700
Subject: CCD FOR FRET

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We hope to purchase a new sensitive CCD and one future application is FRET.
I have no experience with this technique. Please advise me about the time
between the two acquired images, ie. how fast should the system operate?
Thanks

Larry Ackerman
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Avenue
San Francisco, CA 94143 USA

415-476-8751 FAX 415-476-5774

http://www.ucsf.edu/jan



From daemon Wed Jun 5 16:15:41 2002



From: Hong Yi :      hyi-at-emory.edu
Date: Wed, 5 Jun 2002 17:09:38 -0400 (EDT)
Subject: Embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:
I recently did a pre-embedding double immunolabeling run on 50 um
rat brain vibratome sections. The labeling looked beautiful on LM so I
flat embedded sections for EM. Our standard protocol for resin
infiltration and embedding involves placing sections in a mixture of
propylene oxide and Eponate 12 (Pella) at 1:1 ratio in a aluminum dish
overnight, followed by placing sections in 100% Epon for a few hours, and
then embedding with fresh Epon. It has always worked fine for us.
However, I used Epon resin from a different source this time, and am
having problem sectioning. Thin sections disintegrate once they are
floating on water. I have tried to leave embedded tissue in oven for a
few extra days and radiate blocks with UV light. But so far nothing
helped. Does anyone have any suggestion on what else I can try to rescue
these embedded sections, or I should just give it up and start another
run?


Hong



From daemon Wed Jun 5 17:08:52 2002



From: Scott Henderson :      Scott.Henderson-at-mssm.edu
Date: Wed, 05 Jun 2002 18:01:19 -0400
Subject: technical position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A technical position is available in the Microscopy Shared Resource
Facility at Mount Sinai School of Medicine in New York. The
successful candidate will participate in microscopy studies of
various biological systems. Duties include instructing and assisting
users of the facility, sample preparation, image analysis and minor
equipment maintenance. Applicants should have excellent
communication and organizational skills, an understanding of basic
laboratory procedures, and the ability to manage a large and varied
workload. Qualifications include a degree in Biology/Life Sciences,
experience with laser scanning microscopy (confocal and/or
multi-photon), sample preparation (e.g. immunofluorescence), digital
imaging, image analysis and routine equipment maintenance. Computer
skills are essential. Some experience with electron microscopy is an
asset.

For consideration, please mail/email your resume to:

Scott Henderson, Ph.D.,
Director, Microscopy Shared Resource Facility,
Box 1007,
Mount Sinai School of Medicine,
One Gustave L. Levy Place,
New York, NY 10029-6574

email: Scott.Henderson-at-mssm.edu

"We are an equal opportunity employer and foster diversity in the workplace."



From daemon Wed Jun 5 17:32:05 2002



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Wed, 5 Jun 2002 18:28:37 -0400
Subject: RE: repair for old ISI sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

While looking at the operation manual for the E5000, I saw that the machine
will not start operation until the pirani gauge goes below 0.2 Torr.
Assuming that you pumped your system down to at least that level, then
something is wrong with the pressure relay that controls the high voltage or
some part of that circuit is not tripping the relay.

The manual says that you should be able to hear a click when the relay kicks
in at about 0.2 Torr.

Bruce Girrell



From daemon Wed Jun 5 18:39:42 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 5 Jun 2002 17:38:41 -0600
Subject: virus alert

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Relax, Sergey,

you were probably hit by the W32.Klez.H-at-mm worm or virus. As a twist it does
not use the real senders email address, but "fakes" the email address of
some other person in the senders email list. So you got the email from
someone, who has the listserver somewhere in his address book or wherever
the worm gets its information. In other words: This email came from John
Doe, who at some point had contact with the listserver and also has your
email address somewhere.

I don't think you can get the worm through the list server, to my knowledge
it requires some form of html email, which is not accepted on the server. Is
that right, Nestor?

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, June 05, 2002 1:13 PM
To: microscopy-at-sparc5.microscopy.com


I just got message with subject "Subject: To call in the service
representative." from microscopy-at-sparc5.microscopy.com with attachment
aa_160x100[1].exe

This message contains some virus. Unfortunately my Norton Antivirus
eliminate it so quickly and I have no chance to find what virus it was.
Thanks for such nice gift. May be it's sort of sign, I am talking too much
on ListServer? Sergey
------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Wed Jun 5 19:52:01 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 05 Jun 2002 17:46:40 -0700
Subject: Re: virus alert

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Set up Norton to put any virus into quarantine at your
request. Each msg that has a virus will come up with
a Norton warning screen and ask if you want to quarantine
the msg (assuming that repair failed). If the virus could
be repaired, this msg stream does not appear. That could
explain why things happened so quickly.

I am using NAV System Works 5.02 (2002) and it works
great. Be sure to keep the virus definitions updated via
live update (tm).

gary g.


At 12:12 PM 6/5/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jun 5 20:44:29 2002



From: Helvey, Marc :      Marc.Helvey-at-vlsistd.com
Date: Wed, 5 Jun 2002 18:37:49 -0700
Subject: AFM calibration - standards and procedures

Contents Retrieved from Microscopy Listserver Archives
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Hi Matthew -

http://www.vlsistandards.com/products/so_surfTopStan.asp?cid=3&sid=47

Please see the traceability chart at the bottom of the page. Feel free to
contact me offline about this product.

Regards -

Marc Helvey
Strategic Accounts Manager
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
Phone: (408) 428-1800, ext. 108
FAX: (408) 428-9555
Mobile: (408) 221-7581
E-Mail: marc.helvey-at-vlsistd.com {mailto:marc.helvey-at-vlsistd.com}
Internet: http://www.vlsistandards.com




-----Original Message-----
} From: Stephenson, Matthew [mailto:stephenson-at-impactanalytical.com]
Sent: Wednesday, June 05, 2002 9:40 AM
To: Microscopy-at-sparc5.microscopy.com


Greetings all!

I'm in the process of resuscitating a TopoMetrix TMX 2000 with Discoverer
stage for contact and non-contact AFM. We use three scanners: a 70 micron
tripod, a 7 micron tube, and a 0.76 micron tube. The instrument is up and
running, so the time has come to calibrate, incorporate into our quality
program, and get some customers. My questions are as follows:

1. What reference standards are recommended for x, y, and z distance
calibration? Is anything available that is NIST traceable, or barring that,
certifiable by a reputable authority?

2. Have widely accepted AFM calibration procedures emerged in the
microscopy community? I'd be particularly interested in citations for sound
calibration procedure, and hoary wisdom regarding the effect of instrument
variables on the calibration process and accuracy.

I look forward to your comments!

Matthew K. Stephenson
Analytical Associate
Impact Analytical
1910 West Saint Andrews Road
Midland, MI 48640
(989) 832-5555 X506
stephenson-at-impactanalytical.com




From daemon Wed Jun 5 21:01:28 2002



From: georgiana_sprott-at-aemail4u.com
Date: Wed, 05 Jun 0102 23:27:40 +0200
Subject: BIZ, .INFO, .COM for only $14.95

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From: georgiana_sprott-at-aemail4u.com
Date: Wed, 05 Jun 0102 23:27:40 +0200
Subject: BIZ, .INFO, .COM for only $14.95

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From daemon Wed Jun 5 21:18:51 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Wed, 05 Jun 2002 22:10:01 -0400
Subject: RE: TEM imaging plate technology

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First - ElectroImage will probably be selling the Ditabis Imaging Plate
system - but even if that were not true there should be some reality check
in all this - It is beginning to sounds very suspiciously like "Oh my god ,
we can't have another competitor." There are times when the plusses of a
CCD system are the right answer for the TEM digitization problem. Other
times there may actually be some different solution - scan the negatives,
shoot small portions of the negatives with a digital camera and a light
box, or maybe even use an imaging plate system. "One size does not fit
all." If it's all you have to sell, then it is right and proper to defend
it but to spread what you "think" without knowing is particularly bad
science and not particularly helpful to anyone. From the hail storm of
resistance though , you are not alone.


} Careful: the following is an opinion of a CCD system manufacturer. Reader
} discretion is advised!
}
} If I remember correctly, the manufacturers for he imaging plates claim a
} dynamic range of 20 bit, which is considerably more than the 12 or 14 bit
} from a CCD camera. If that, however, makes a big difference for diffraction
} patterns I don't know.

Then why bring it up?

} If you want to record the weakest spots, you need to
} expose at least for a time so that the signal is above noise. Since CCD
} cameras are very sensitive (down to single electron sensitivity), there is
} not much that can be improved by the imaging plates.

Imaging plates lack the readout noise of CCD's . It is true that there are
CCD's that can detect single photons but to my knowledge no one uses them
in this application. Certainly the new photon to electron cameras fit into
this category. Having higher bit depth means not only that you can "see"
the dimmer spots but that you can miss the exact exposure and still get
good images. The high DQE at low dose minimizes the exposure as does
the the large image area. Additionally, software like Lucis is ideally
suited to extract tremendous detail from just such high bit depth images.


} There are no half
} electrons (and if you see 1/3 electrons, book a flight to Stockholm).

It's DQE that counts here. Imaging plates have a DQE of .8 as low as
.001e/cm squared. What is the DQE of a CCD camera at low dose exposures?
The answer is



} If the
} 20 bits are sufficient to image the weakest beams together with the
} transmitted beam without saturation I don't know. With the Imaging plates
} you can probably get the weakest spots and more medium intensity spots at
} the same time than with a CCD. With a CCD you have the advantage that you
} immediately see the result and can adjust your exposure accordingly.


And if the sample is beam sensitive, then what? Again, there is no one
right answer.

} It's
} also very easy to take exposures at different exposure times and combine
} them, as the CCDs are very linear.


The imaging plates are linear over their six order of magnitude range -
CCDs as well as film has a sigmoid curve response and are linear only over
the central portion of their total response range.


} I can't remember anything about linearity of the imaging plates. But the way
} they work (excite crystals into a semi-stable state through the light from a
} phosphor and then "read' them by relaxing into ground stage through a
} laser), I would think there are some non-linearities as with increasing
} exposure you have less and less crystallites that can be excited. Whether
} that's critical for quantitative measurements I can't say.


And, indeed, you are quite wrong.


} Finally, the images on the plates are stable for a few hours. So you can't
} leave the plates in for a few days if you want the best results.

That is correct.



} One area, where in my experience (as a TEM user) CCD cameras have a big
} advantage is dark field imaging. I remember taking several films with
} different exposures to make sure that I had one that was right. With a CCD
} there is no guesswork anymore, as you can see the images immediately.


The extended dynamic range of the imaging plates pretty much negates this
re-exposure scheme.


} Resolution: theoretically the plate film should have a worse resolution that
} film. The electron beam has to travel through the crystallite layer, create
} light in the phosphor layer, and the photons then have to travel back to the
} crystallites to excite them. This should add to the dispersion of the beam,
} resulting in a reduced resolution. The manufacturers of the imaging plates
} claim around 20 microns resolution. If that is a 20 micron resolution at 20
} bit dynamic range they don't say.


15um resolution with .4MTF at the Nyquest frequency


} Again, we produce those CCD systems, and I definitely have a bias. But I
} thought I put my 2 cents in.
}
} Mike


The imaging plates offer high resolution over a large area - not quite the
resolution of film but the same area and with better sensitivity, higher
dynamic range and much better linearity. With up to 6000 x 5000 pixels (
actually from 1800 x 1250 to 6000 x 5000) the imaging plates offer much
more resolution for much less money than any other commercially available
system. They are not perfect but they can represent a solution to replace
film to produce high quality digital images. My position is to try to offer
the best solution for the customers problem. There are times when a CCD
system is the absolute right solution, as pointed out by Dr. Daberkow, but
there are times when it may not be. Now there is a choice.

Bill Miller
ElectroImage

} } } } } } } } } } } WE HAVE MOVED { { { { { { { { {
} please make a note of the new address below
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
} Sent: Wednesday, June 05, 2002 7:23 AM
} To: 'Sergey Ryazantsev'; Microscopy-at-sparc5.microscopy.com
} Subject: RE: TEM imaging plate technology
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jun 5 21:39:48 2002



From: zaluzec-at-microscopy.com
Date: Wed, 5 Jun 2002 21:30:12 -0500
Subject: Administrivia: Virus Alert

Contents Retrieved from Microscopy Listserver Archives
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Colleagues

Yes, Mike Bode and the othere that have replied are correct.
The listserver is setup to reject any messages with attachments.
These include EXE, HTML, and a host of others all of which
carry virus or are suspect/junk mail. If any valid mail
is caught by the filter and it does happen. Please read the directions in the
"returned message" , follow the instructions and we
can get your message through to the list.

The latest virus W32.Klez.H-at-mm worm, and variants there of spoof (fake)
email addresses and make someone else look as if someone else is
the "bad guy". This is likely the case with Sergey's mail.

As Warren has pointed out all Listserver mail will
have the banner/heading in the body of the message.
If you get suspect mail which carries the listserver
address and DO NOT see that banner it is guarenteed
to be a spoofed message. However, that being said,
the filters are not perfect and some spam gets through,
like the one on WWW Sites today, which I've now
added to my ever growing spam site list .

Just in case your curious the spam filter stopped 102
junk mail messages in the last week from reaching
all of you... I'll warn you this is a ever growing problem
and I have to continuously fight back the junk... It's
not what I would call fun..

Nestor
Your Friendly (and sometime tired) Neighborhood SysOp



From daemon Wed Jun 5 21:55:58 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 5 Jun 2002 20:56:46 -0600
Subject: RE: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
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Bill,

I don't want to drag this out, you have your opinion and I have mine. But
let me correct you on one thing: CCDs are very linear over their range. If
you don't believe me, then perhaps you want to check out this site:

http://www.roper.co.jp/Html/teflin.htm

Here is a quote: High-performance CCD (HCCD) imagers have extremely good
linearity. Deviations from linearity are often less than a few tenths of a
percent for over five orders of magnitude.

Our measurements show similar results.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Bill Miller [mailto:microbill-at-mohawk.net]
Sent: Wednesday, June 05, 2002 8:10 PM
To: Mike Bode; 'Microscopy-at-MSA.Microscopy.Com'


From daemon Wed Jun 5 22:41:19 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 05 Jun 2002 20:36:15 -0700
Subject: Re: virus alert

Contents Retrieved from Microscopy Listserver Archives
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Attachments are not allowed on the MSA listserver. Nestor
does a great job of keeping postings true to this rule. If you
do have an attachment post that slips through, you probably
should update Nestor about it. Send your header info to him
if possible to help him differentiate your situation from others.

Nevertheless, Norton AV will trap any known virus in the body
or attachment of an e-mail message--or web-based thread.
Lately, I'm getting klez and sircam viruses. these are old and
well-known pathogens. Now, simply a pain.

Some simple (not free) actions for a virus-free environment is
to dump Microsoft Outlook and web browsers in favor of Qualcomm's
Eudora e-mail client and get Norton Anti-Virus (part of Norton System Works,
which includes other good options) and do not open attachments from
anyone you do not know. Beware of .SCR attachments (screen
savers). The central theme is to use Eudora for e-mail rather
than Netscape or IE5 or IE6 browsers or Outlook. You can also get relief
from viruses on the usenet by not using the browsers and instead,
using ForteInc Agent newsreader....very nice (using it for 5 years).

gary g.


At 12:12 PM 6/5/2002, you wrote:

} I just got message with subject "Subject: To call in the service
} representative." from microscopy-at-sparc5.microscopy.com with attachment
} aa_160x100[1].exe
}
} This message contains some virus. Unfortunately my Norton Antivirus
} eliminate it so quickly and I have no chance to find what virus it was.
} Thanks for such nice gift. May be it's sort of sign, I am talking too
} much on ListServer? Sergey
} ------------------------------------------------------
}
} Sergey Ryazantsev, Ph.D.
} Electron Microscopy
} Department of Biological Chemistry, School of Medicine
} University of California, Los Angeles
} Box 951737
} Los Angeles, CA 90095-1737
}
} (310) 825-1144 (office)
} Pager: (310) 845-0248
} FAX: (310) 206-5272 (departmental)
} mailto:sryazant-at-ucla.edu
}



From daemon Thu Jun 6 04:12:32 2002



From: Khalid Boulahya :      khalid-at-quim.ucm.es
Date: Thu, 06 Jun 2002 11:02:42 +0200
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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subscribe

we are looking urgently for a stage double tilt holder for EM400 family or
CM Philips microscopes (not compustage holder) for reasonable price.

best regards



From daemon Thu Jun 6 06:54:12 2002



From: Khalid Boulahya :      khalid-at-quim.ucm.es (by way of MicroscopyListserver)
Date: Thu, 6 Jun 2002 06:42:55 -0500
Subject: TEM Stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear friends,

we are looking urgently for a stage double tilt holder for EM400
family or CM Philips microscopes (not compustage holder) for
reasonable price.

best regards

**************************************************************
Khalid Boulahya
Depto de Química Inorgánica
Facultad De Químicas
Universidad Complutense de Madrid (UCM)
28040 Spain
khalid-at-quim.ucm.es
**************************************************************


From daemon Thu Jun 6 07:42:34 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 6 Jun 2002 08:35:21 -0400
Subject: RE: Embedding

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Morning Hong,
The last time I had such a problem, I remember that I immediately
tried to section one of the blank blocks I always form from new batches of
resin. In my case, that block belied my feeling, after my first blank run
with the new resin, that I could trust the formulation of the new stuff. In
that instance, I had to go back and set up another run, after I practiced a
couple more times with the new furmulations.

My current rules: 1) Always make certain that a new type of
resin works by itself before you commit tissue to it. I don'/t like
sectioning blanks either, but the procedure helps to prevent REAL problems.

2) Always keep 1-5 10ml syringes of
pre-mixed resin from an old batch that really works so that when someone
comes along with a specimen that can't be replaced, you have a reliable
embedment to use.
3) Always mix your own stuff. My
experience has been that whenever a technician begins to mix things that
always work, you have to start looking for a replacement.

Sorry for your loss,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Hong Yi
} Sent: Wednesday, June 5, 2002 5:09 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Embedding
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All:
} I recently did a pre-embedding double immunolabeling run on 50 um
} rat brain vibratome sections. The labeling looked beautiful on LM so I
} flat embedded sections for EM. Our standard protocol for resin
} infiltration and embedding involves placing sections in a mixture of
} propylene oxide and Eponate 12 (Pella) at 1:1 ratio in a aluminum dish
} overnight, followed by placing sections in 100% Epon for a few hours, and
} then embedding with fresh Epon. It has always worked fine for us.
} However, I used Epon resin from a different source this time, and am
} having problem sectioning. Thin sections disintegrate once they are
} floating on water. I have tried to leave embedded tissue in oven for a
} few extra days and radiate blocks with UV light. But so far nothing
} helped. Does anyone have any suggestion on what else I can try to rescue
} these embedded sections, or I should just give it up and start another
} run?
}
}
} Hong
}
}
}


From daemon Thu Jun 6 08:46:57 2002



From: Young, Gene (GP) :      GPYOUNG-at-dow.com
Date: Thu, 6 Jun 2002 09:38:51 -0400
Subject: Re:TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
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Sergey,

Can you give more details on how your Gatan system is setup to broadcast
live over a network? Are you using something like Netmeeting? I am very
interested in doing this.

Gene

Gene P. Young
Research Technologist
Analytical Sciences, Polymer Characterization

The Dow Chemical Company
2301 N. Brazosport Blvd., B-1470
Freeport, Texas 77541-3257
Fax: (979) 238-0095
Phone: (979) 238-1579


-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Monday, June 03, 2002 11:02 PM
To: Microscopy-at-sparc5.microscopy.com

{snip}
-another thing is academic: The live image from digital camera I broadcast
to the Departmental network, so you could see live image on any
departmental computer. PIs now comfortably sit in their offices and enjoy
watching how their students working on the TEM. Similarly it works for
Internet. We did a few session when our collaborators observe their
samples sitting far-far away from UCLA.

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jun 6 08:46:57 2002



From: Jamila Shawon :      Jamila_Shawon-at-student.uml.edu
Date: Thu, 06 Jun 2002 09:38:14 -0400
Subject: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
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Dear all:

recently I am trying to prepare TEM sample of CMP polyurethane pads. I
made the sample with embaded resin and did microtoming. After
microtoming, I did staining by RuO4 (as my purpose is to identfy the
hard and soft phases). But my problem is that I have no idea how the
stained polyurethane will look like. I tried several times, but it was
hard to distinguish the sample from resin. So does anyone have any idea
about the PU cmp pads TEM images after staining? Please let me know.

Thanks
Jamila Shawon



From daemon Thu Jun 6 08:58:41 2002



From: Lou bustillos :      lbustillos-at-amalab.com
Date: Thu, 6 Jun 2002 09:52:35 -0400
Subject: Chillers

Contents Retrieved from Microscopy Listserver Archives
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Hello,

First I would like to say thank you to everyone that helped me with my last
email about purchasing a carbon coater. Hopefully installation will be in
the next two weeks.

My new question is about chillers for a JEOL 100CX II. The state that we
are located in is beginning to have a drought and we want to do our part by
changing our water chillers to air cooled chillers. I would like to know
your experience with switching to an air cooled system. Also, what
manufacture would you recommend? I have two 100CX II and they will have
there own separate units. Thank you for your help.



From daemon Thu Jun 6 09:08:50 2002



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 6 Jun 2002 10:03:44 -0400
Subject: TEM Stage

Contents Retrieved from Microscopy Listserver Archives
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Contact:

Optechs, Inc.
Used Philips parts
Dick Turnage
446 Acapesket Road
East Falmouth, MA 02536
617 739-7900 voice
978 667-1827 fax


}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
Storrs, CT 06269-2242
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Thu Jun 6 10:03:19 2002



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Thu, 06 Jun 2002 16:54:33 +0200
Subject: Re: TEM imaging plate technology

Contents Retrieved from Microscopy Listserver Archives
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Sergey, Bill, Mike,

After I have been contacted by a listener off-line, I want to correct my remark:
“A normal bright-field image has typically a dynamic range of about 100…”

First at all, the expression “dynamic range” has to be replace by “signal-to-noise
ratio” (SNR) – sorry for my fault. It makes only sense for a detector not for an
image itself.

Secondly, the value 100 was estimated by 10,000 incident electrons per pixel –
resulting in a noise 100 = sqrt(10,000). Therefore the SNR of a captured image is
about 100 (=10,000/100) – assuming a “normal” specimen (e.g. weak-phase object).
But please don’t pin me down at numbers, basically I wanted to express that for
normal (bright-field) applications an image detector with a dynamic range of a few
hundred is sufficient. Actually a photo plate has “only” such a dynamic range.
But to make it clear: A SNR of 100 is an excellent value – a rough rule of thumb
says the SNR should be at least higher than 5 (so-called “Rose criteria”). I don’t
dare to give a reference of this old and famous work from 1946 :-)

I feel that this question may arise: “But why are you guys selling CCD cameras
which such a high dynamic range if normal users don’t need it?”
A: Normally, CCD cameras are designed to detect single electrons – especially for
low-dose/cryo applications. If you want to capture a “high-dose” image with 10,000
electrons/pixel, then the huge dynamic range is needed. This means CCD cameras are
able to handle both applications.

One remark regarding Bill (and Mike): I agree with Mike, the non-linearity of CCD
cameras is normally less than 1%.

Best wishes,
Ingo

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Dr. Ingo Daberkow
Tietz Video and Image Processing Systems GmbH
Eremitenweg 1
D-82131 Gauting, Germany
Tel: +49-89-8506567
FAX: +49-89-8509488
Internet: http://www.tvips.com/
Email: ingo.daberkow-at-tvips.com
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++




Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Ingo
} Thanks for comments. I absolutely agree with folks who mentioned extremely
} wide dynamic range for image plates. I think there is some misunderstood
} happening: In order to get data from image plate, you have to scan it and
} record it as a digital image. The quality of scanners is different, some
} of them could not provide 20 bit gray scale. When I pointed, that dynamic
} range is the same for the images from CCD and IP I mean that the images
} were recorded in 12-16 bit format, which is very usual for nova days. 20
} bit format is non-readable by most software and therefore useless for such
} simple person like me, who is still using Photoshop for most jobs. So,
} from this point of view, we have practically the same dynamic range of the
} final digital image. I am sorry if my posting confuse somebody. Image
} plate technology widely used in X-ray crystallography and as a substitution
} for X-ray film in molecular biology applications for decades. No question
} about that: this is a great technology. BUT, my point, is that current
} realization of this technology in EM does not have serious advantages over
} normal film/digital camera combination. AND: keep in mind, how long you
} will scan to get those 20 bits dynamic range... Thanks again for the very
} good point. Sergey
}
} At 01:13 PM 6/5/02, you wrote:
} } Dear Sergey,
} }
} } Just another remark regarding the dynamic range. The imaging plates have a
} } much
} } higher dynamic range than other electron detectors (up to 20 bit after several
} } scans). But please notice the dynamic range is defined as: (maximum signal/RMS
} } noise) ­ this is not the digitization level of the analog-to-digital
} } converter.
} } This extreme high dynamic range is only necessary for the acquisition of
} } diffraction patterns. A “normal” bright-field image has typically a dynamic
} } range of about 100, which is mainly limited by the noise of the electrons
} } (shot-noise). This means, for such applications CCD cameras have the advantage
} } of a real-time acquisition (WYSIWYG) and furthermore the camera can be
} } used for
} } checking or controlling of the TEM parameters (defocus, astigmatism, etc.)
} }
} } Personally I have found the literature list of Wharton very useful ­ which
} } enables the interesting reader to collect more specific information.
} }
} } Best wishes,
} } Ingo
} }
} } +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} } Dr. Ingo Daberkow
} } Tietz Video and Image Processing Systems GmbH
} } Eremitenweg 1
} } D-82131 Gauting, Germany
} } Tel: +49-89-8506567
} } FAX: +49-89-8509488
} } Internet: http://www.tvips.com/
} } Email: ingo.daberkow-at-tvips.com
} } +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} }
} } ,
} }
} }
} }
} } "Sinkler, Wharton" wrote:
} }
} } } ------------------------------------------------------------------------
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} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} } } -----------------------------------------------------------------------.
} } }
} } } OK, Truce Sergey!
} } }
} } } Your point is well taken, and yes, design considerations and
} } convenience may
} } } overwhelm a good MTF or linearity.
} } }
} } } However, I think you are mistaken about dynamic range being similar for
} } } Imaging Plates and CCD's. That being said, I have gone back to look at the
} } } papers I mentioned and I'm not able to find a clear statement of what I
} } } mean.
} } }
} } } I believe (maybe someone will set us straight on this) that the saturation
} } } of the IP is considerably higher than the CCD. By 'saturation' I mean the
} } } dose (electrons/unit area) at which there is significant deviation from
} } } linearity.
} } }
} } } The reason saturation level is important (and why the IP is great for
} } } diffraction) is that signal/noise at low electron doses is dominated by
} } shot
} } } noise. So if you are trying to record a diffraction pattern with a CCD
} } with
} } } a single exposure without saturating the CCD at the strong spots, the weak
} } } reflections will be noisy simply because relatively few electrons are
} } } arriving there (statistical or 'shot' noise). With a higher saturation (I
} } } believe the case for IP), you can expose longer and get better statistics
} } } overall. (Of course you can also record multiple exposures with the CCD at
} } } different times and merge the data).
} } }
} } } Regards,
} } } Wharton
} } }
} } } } -----Original Message-----
} } } } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } } } Sent: Tuesday, June 04, 2002 7:42 PM
} } } } To: Microscopy-at-sparc5.microscopy.com
} } } } Subject: RE: TEM imaging plate technology
} } } }
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Dear Wharton
} } } }
} } } } Are you seriously think, people will read that very specific papers? Do
} } } } you think, so many of us is ready to go through all this math etc? I
} } } } agree,
} } } } it's very important to understand the scientific foundation for
} } device you
} } } }
} } } } are going to use. But, in practice you have deal not with such nice
} } } } things
} } } } as PSF (point spread function) or MTF. The camera with very nice PSF may
} } } } have terrible design and not suitable for use. Company, who manufactured
} } } } that baby may not respond on your telephone calls and finally you may
} } } } figure out that it does not exists anymore. I do read scientific
} } } } journals
} } } } and it's extremely easy to find informations on the Internet nova days,
} } } } ListServer to me is a place where we have chance to share our personal
} } } } experience (bad or good), which mostly is not published and not
} } indexed by
} } } }
} } } } Medline/CC whatever. Anyway, thank you for the reference, I enjoyed
} } } } reading it. Sergey
} } } }
} } } } At 01:41 PM 6/4/02, you wrote:
} } } }
} } } } } Sergey et al,
} } } } }
} } } } } I'd just like to point out that instead of relying on uncertain
} } } } } recollections, there is some good work in the literature which discusses
} } } } } both slow-scan CCD's and imaging plates. Here's a start:
} } } } }
} } } } } J. M. Zuo "Electron detection characteristics of slow-scan CCD camera"
} } } } } Ultramicroscopy 66 (1996) 21-33.
} } } } }
} } } } } J. M. Zuo, M. R. McCartney and J. C. H. Spence "Performance of imaging
} } } } } plates for electron recording" Ultramicroscopy 66 (1996) 35-47
} } } } }
} } } } } G. Y. Fan and M. H. Ellisman "Digital imaging in transmission electron
} } } } } microscopy" Journal of Microscopy 200 (2000) 1-13.
} } } } }
} } } } } Although the technology is continuously evolving, the basic points are
} } } } still
} } } } } valid.
} } } } }
} } } } } Regards,
} } } } } Wharton
} } } } }
} } } } } *************************************************
} } } } } Wharton Sinkler, Ph.D.
} } } } } UOP LLC
} } } } } 25 E. Algonquin Rd.
} } } } } Des Plaines, IL 60017-5017
} } } } } 847-391-3878
} } } } }
} } } } }
} } } } } } -----Original Message-----
} } } } } } From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} } } } } } Sent: Tuesday, June 04, 2002 3:36 PM
} } } } } } To: microscopy-at-sparc5.microscopy.com
} } } } } } Subject: Re: TEM imaging plate technology
} } } } } }
} } } } } }
} } } } ------------------------------------------------------------------------
} } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } } America
} } } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } }
} } } } -----------------------------------------------------------------------.
} } } } } }
} } } } } }
} } } } } } Dear Donald
} } } } } }
} } } } } } Before I bought the digital camera, I was looking on that image plate
} } } } } } technology. It seems to me, it does not have serious advantage for
} } } } most
} } } } } } of
} } } } } } us. I would like to concentrate my findings in a few sentences.
} } } } } }
} } } } } } Image plate PRO:
} } } } } } - has more pixels that most digital cameras (some top-end cameras
} } } } } } HAS similar amount).
} } } } } } - has dynamic range same as digital cameras 12-16 bit.
} } } } } } - it's more sensitive than film.
} } } } } } - very good for electron diffraction experiments!!! (only one real
} } } } plus to
} } } } } } me).
} } } } } } - manufacturers claimed that pixel resolution on the plate is
} } } } comparable
} } } } } } with film (it's difficult to proof because you have to scan film to
} } } } } } compare, so it'll depend from the film scanner).
} } } } } }
} } } } } } Image plate CONTRA:
} } } } } } Imitate the film procedure - you have to perform all procedures
} } as for
} } } } } } film, load/upload plates into cassettes (in the dark), change the
} } } } magazine
} } } } } }
} } } } } } (wait for vacuum), load plates into the scanner (in the dark I
} } } } believe),
} } } } } } wait for scanning - 2 (or more, don't remember, up to 5 at full
} } } } resolution
} } } } } }
} } } } } } I believe) min etc. So, it does not eliminate the dark-room,
} } scanning
} } } } is
} } } } } } slow and then you have to process/save/print data. Time consuming.
} } } } You
} } } } } } have all disadvantages the classical film use: plate may be scratched
} } } } } } during loading/uploading, deformed, lost, dropped on floor with
} } } } valuable
} } } } } } image etc. One plate is $100 I believe. No practical use in most
} } } } } } biological applications I believe. I am not warranty that all my
} } } } } } information is current, I was shopping for image plate system a few
} } } } years
} } } } } } ago and my comments represent the situation of that time. Sorry,
} } } } } } manufacturing gays. Sergey
} } } } } }
} } } } } } At 08:07 AM 6/4/02 -0500, you wrote:
} } } } } }
} } } } } ------------------------------------------------------------------------
} } } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } } America
} } } } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } }
} } } } } -----------------------------------------------------------------------.
} } } } } } }
} } } } } } }
} } } } } } }
} } } } } } }
} } } } } } } Dear EM Netters,
} } } } } } }
} } } } } } } Does anyoun out there have experience with imaging plate technology
} } } } (i.e.
} } } } } } } Ditabis)? I
} } } } } } } would like to hear pros and cons concidering this technology. How
} } } } does
} } } } } } it
} } } } } } } compare to
} } } } } } } digital CCD camera systems (cost, time, supplies, reliability. We
} } } } are
} } } } } } } looking into possibly
} } } } } } } retrofitting our JEOL 100CX with an off-line system.
} } } } } } }
} } } } } } }
} } } } } } } Thanks in advance
} } } } } } }
} } } } } } }
} } } } } } } Donald G. Awbrey, HT (ASCP), QIHC
} } } } } } } Electron Microscopy / Image Analysis
} } } } } } } 817-878-5647
} } } } } } } donaldawbrey-at-texashealth.org
} } } } } } }
} } } } } }
} } } } } } ------------------------------------------------------
} } } } } }
} } } } } } Sergey Ryazantsev, Ph.D.
} } } } } } Electron Microscopy
} } } } } } Department of Biological Chemistry, School of Medicine
} } } } } } University of California, Los Angeles
} } } } } } Box 951737
} } } } } } Los Angeles, CA 90095-1737
} } } } } }
} } } } } } (310) 825-1144 (office)
} } } } } } Pager: (310) 845-0248
} } } } } } FAX: (310) 206-5272 (departmental)
} } } } } } mailto:sryazant-at-ucla.edu
} } } } } }
} } } }
} } } } _____________________________________
} } } }
} } } } Sergey Ryazantsev Ph. D.
} } } } Electron Microscopy
} } } } UCLA School of Medicine
} } } } Department of Biological Chemistry
} } } } Box 951737
} } } } Los Angeles, CA 90095-1737
} } } }
} } } } Phone: (310) 825-1144
} } } } FAX (departmental): (310) 206-5272
} } } } mailto:sryazant-at-ucla.edu
} } } }
} } } }
} } } }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu



From daemon Thu Jun 6 10:23:02 2002



From: Battjes, Kevin :      battjes-at-impactanalytical.com
Date: Thu, 6 Jun 2002 11:16:14 -0400
Subject: Chillers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lou,
We have had Haskris chillers, both water cooled and air cooled for many
years. They run 24/7 very faithfully. I would buy another one.

Disclaimer: Haskris has never bought me lunch or coffee or even a candy
bar! I have no commercial interest in the sales of their equipment.


Kevin Battjes
Impact Analytical Voice 989-832-5555, ext 556
Michigan Molecular Institute Fax 989-832-5560
1910 W. St Andrews Road e-mail: battjes-at-mmi.org
Midland MI 48640 battjes-at-impactanalytical.com




-----Original Message-----
} From: Lou bustillos [mailto:lbustillos-at-amalab.com]
Sent: Thursday, June 06, 2002 9:53 AM
To: Microscopy-at-sparc5.microscopy.com


Hello,

First I would like to say thank you to everyone that helped me with my last
email about purchasing a carbon coater. Hopefully installation will be in
the next two weeks.

My new question is about chillers for a JEOL 100CX II. The state that we
are located in is beginning to have a drought and we want to do our part by
changing our water chillers to air cooled chillers. I would like to know
your experience with switching to an air cooled system. Also, what
manufacture would you recommend? I have two 100CX II and they will have
there own separate units. Thank you for your help.



From daemon Thu Jun 6 12:56:36 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 06 Jun 2002 13:41:08 -0400
Subject: Re: Chillers

Contents Retrieved from Microscopy Listserver Archives
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Lou-

Air cooled chillers work just fine. They produce lots of heat, so they
will need good ventilation in the summer. However, they cannot be in a
position where they would freeze in winter if switched off for any reason
(e.g. power failure). They also tend to be quite noisy.

We have systems from several manufacturers. They all have been good. I
would shop around for the usual best combination of performance, features
and price appropriate for your application.

It doesn't apply to you, but a user switching from using the public water
supply for direct cooling will have been using a system with extremely good
short-term temperature stability. A simple chiller does not have that -
typically it will vary by about 2ºC over its cycle time - i.e. a few
minutes. This will usually be very clear in the image drift! There are a
number of methods of improving the short-term temperature stability which
can be discussed with the chiller manufacturers.

We have always used recirculating chillers, using water-cooled models. We
switched several years ago from the city water as the coolant for the
condenser to the chilled water provided by the facilities people for the
air conditioning. After a few teething troubles (which I would be glad to
share with anyone interested) our system now works reliably, doesn't waste
water, doesn't dump heat into the rooms, isn't too noisy, and didn't
require us to buy 6 new air-cooled chillers! Of course not everyone will
have that option.

Tony.



} Hello,
}
} First I would like to say thank you to everyone that helped me with my last
} email about purchasing a carbon coater. Hopefully installation will be in
} the next two weeks.
}
} My new question is about chillers for a JEOL 100CX II. The state that we
} are located in is beginning to have a drought and we want to do our part by
} changing our water chillers to air cooled chillers. I would like to know
} your experience with switching to an air cooled system. Also, what
} manufacture would you recommend? I have two 100CX II and they will have
} there own separate units. Thank you for your help.


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Thu Jun 6 13:53:13 2002



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Thu, 6 Jun 2002 14:49:07 -0400
Subject: Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor:

I really enjou the listserver, and think I get a great deal of good
stuff from it. I think you do a great job managing it, and thank you
immensely for all the effort you put into doing so.

Best regards,
Wil Bigelow
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Thu Jun 6 13:53:15 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 06 Jun 2002 11:59:14 -0700
Subject: RE: virus alert

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fred!

Thanks for your message. I really appreciate your respond. It's so nice
of you. Of coarse, I never ever intend to think that somebody from
ListServer sent to me virus. It was sort of joke (my 'humor' was a little
bit heavy, I guess). As a matter of fact, I survived a few complete (!)
computer crushes with following HD reformat. In all that cases I got
viruses from friends (lost attention). So, applying this idea to our
situation: everyone on ListServer is my friends! And it's very
true. ListServer become to me a place where friends are situated. I am
really sorry if my last posting somehow hurt our ListServerers. I really
enjoy being a part of this society and thank you so much for attention.
Sergey


} } ----------
} } From: Sergey Ryazantsev
} } Sent: Wednesday, June 5, 2002 3:12 PM
} } To: microscopy-at-sparc5.microscopy.com
} } Subject: virus alert
} } Importance: High
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I just got message with subject "Subject: To call in the service
} } representative." from microscopy-at-sparc5.microscopy.com with attachment
} } aa_160x100[1].exe
} }
} } This message contains some virus. Unfortunately my Norton Antivirus
} } eliminate it so quickly and I have no chance to find what virus it was.
} } Thanks for such nice gift. May be it's sort of sign, I am talking too
} } much
} } on ListServer? Sergey
} } ------------------------------------------------------
} }
} } Sergey Ryazantsev, Ph.D.
} } Electron Microscopy
} } Department of Biological Chemistry, School of Medicine
} } University of California, Los Angeles
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } (310) 825-1144 (office)
} } Pager: (310) 845-0248
} } FAX: (310) 206-5272 (departmental)
} } mailto:sryazant-at-ucla.edu
} }
} }
} }

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Thu Jun 6 15:40:53 2002



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Fri, 7 Jun 2002 08:31:30 +1200
Subject: re-embedding

Contents Retrieved from Microscopy Listserver Archives
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Hi Hong

Dug out and dusted off these references for re-embedding last night.


1. Method of re-embedding tissue for electron microscopy. Stain
Technology Vol49, page 118 to 119. (1974) Bauman and Mendall

Done with Spurrs.

- Trimmed away excess plastic under a stereo microscope with razor blade
- tissue put in vial of Propylene Oxide and sonicated for 1 hour
- excess plastic (now soft and pliable) again trimmed away
- tissue infiltrated with 1:1 resin/ propylene oxide with gental
agitation for 18 hours at RT
- 48 hours infiltration in full strength Spurrs, again gentle agitation at RT
- re-embed and polymerise

We have tried this with tissue blocks and it worked for us. Don't
know how vibratome slices would stand up to it.



2. Rescuing poorly embeded tissue for electron microscopy: a new and
simple technique for re-embedding. Stain Technology Vol50, page 209
to 211. (1975) McNelly and Hinds

Done with Araldite 502.

- place 10µm (or thin as can get) slices placed in disposable
aluminum weighing dishes
- cover sections with distilled water and heat on hot plate until
water evaporated, leaving sections stuck on the bottom.
- fill dishes with resin mixture and place in a dessicator over night
(doesn't mention vaccum level or use of a desicant)
- then transfer to an oven at 60oC for 48 hours.
- remove polymerised block cut out area of interest, remount and away you go

I haven't tried this one so no comment on its success.


3. I don't have the reference for this one but it is mentioned in
Principles and Techniques of Electron Microscopy, MA Hayat, Third
Edition, page 133

Method of Ogura and Oda, 1973.

-trimed excess plastic away, soaked in 100% ethanol saturated with
KOH for several hours. The ethanol/KOH must be matured overnight
before use (becomes dark brown).

- after solubilisation of the resin soak in 100% ethanol, followed by
propylene oxide, then re-embedded in resin.

Interestingly McNelly and Hinds and say in their paper they tried
this and it didn't work for them. I haven't tried it either so can't
comment.

As I mentioned above, the only one I have tried is 1. above and it
worked for us.

Good luck,

Allan

PS. have you spotted any legs under any bar tables lately that match
or better Doug's yet ?
--
-------------------------------------------------
Allan Mitchell
Technical Manager
Otago Centre for Electron Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Thu Jun 6 17:44:52 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 06 Jun 2002 15:36:13 -0700
Subject: RE: TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
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Dear Gene

I am using VNC software, which is stands for Virtual Network
Computing. It's freeware, look at http://www.uk.research.att.com/vnc/

VNC is developed to transmit ANY information via network, not necessarily
images from digital camera. Actually, it transmits the whole screen in the
real time (depends from your network speed). So, If you are working with
digital camera, it'll transmit everything you doing/seeing. It has a
setting, when remote mouse/keyboard is activated. In this case remote
operator may manipulate your computer/camera/software. If microscope
controlled by computer, you may control the whole things. The reasons, I
am using this software rather than NetMeeting or something like that are:
VNC is very compact and should be installed on the 'transmitter computer'
only (VNC-server, transmitter). It may works as a 'service' or as a
standard application under WinNT/2000 (it does not work under Win95/8). All
other computers - recipients, should run "VNC viewer" - 34 kB .exe file
only (there is no installation, just run). You could run it even from
diskette on any computer (OS limitation). In order to use VNC, you don't
have to use the 'outside server' as it happening with NetMeeting, ICQ etc:
You connected to MS server and it transmit your session to another
'user'. VNC transmits to 'everyone' inside the network, and you may get
this transmission if you run VNC-viewer and provide correct password
(password is not transmitted via network). If you permit transmission to
Internet - everyone on the Internet will have chance to catch this
transmission, this is a disadvantage. For 'Internet' session, I've sent
by E.mail VNC-viewer file, preconfigured for listening my transmission and
password by phone. Remote user should double-click on the VNC-viewer.exe
file and enter password. That's it. Only one things here: remote user
should not be such picky/panic as me about viruses in .exe files.

The disadvantage of VNC is: It designed for local network and therefore has
very limited security (I belive, it uses standard TCP/IP protocol), so the
computer running 'VNC server, read - transmitter' is vulnerable if
available from the Internet. The best way to use VNC is to use it inside
the local network, protected from outside by firewall and never run it on
your 'departmental server'. I am not running VNC permanently, instead, I
set 'session' when my computer is running this software. For each session
I provide a new password. If you have good network administrator, s/he
could probably advise you, how to use this software safely in your
particular case.

I hope it could help. Good luck. Sergey

At 06:38 AM 6/6/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jun 6 23:38:30 2002



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 06 Jun 2002 23:24:07 -0500
Subject: RE: TEM-Digital Camera Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 03:36 PM 6/6/2002 -0700, Sergey Ryazantsev wrote:
} I am using VNC software, which is stands for Virtual Network Computing. It's freeware, look at http://www.uk.research.att.com/vnc/
} It may works as a 'service' or as a standard application under WinNT/2000 (it does not work under Win95/8).

VNC server and viewer works fine under Windows 98.
Also see http://www.tightvnc.com/ . It's also multi-platform,
so someone on a Mac can watch a PC. I've used the VNC viewer
on palmtops and even a Palm, I think.

- John



From daemon Fri Jun 7 02:43:17 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 7 Jun 2002 08:38:17 +0100
Subject: Hitachi / EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is anyone out there using EDAX Phoenix on a Hitachi 4700?
If so, I would very much like to make contact, offline

Dr. Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
EDINBURGH EH9 3JN UK

Inveresk Cottage
26, Carberry Road
Inveresk
Musselburgh
Midlothian
EH21 8PR
Tel: +44 131 665 6062
FAX +44 131 653 6248
Mobile 07710 585 401



From daemon Fri Jun 7 04:35:01 2002



From: PHYSIOL4-at-AKAD.SUN.AC.ZA
Date: Fri, 7 Jun 2002 11:26:26 +0200
Subject: Subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Subscribe


"When life hands you a lemon ... bring out the tequila and salt."


From daemon Fri Jun 7 09:53:39 2002



From: Jeannette Taylor :      jvtaylo-at-emory.edu
Date: Fri, 07 Jun 2002 10:50:32 -0400
Subject: negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have just had an interesting problem show up, or not show up as it
were. One of the students here put her negatively stained grids into
the TEM to re-examin after a couple of months and there appeared to be
no stain. She used K-PTA at pH 2 for 4 minutes and got an a excess of
stain initially. I have not re-viewed negatively stained grids once I
have photographed them so I don't know what might have happened.
Neither have I heard of this before.
Does the List have experience and comments on this? Please do.

Thanks.
Jeannette Taylor
IM&MF/ Emory University
jvtaylo-at-emory.edu



From daemon Fri Jun 7 10:24:11 2002



From: David Hall :      hall-at-aecom.yu.edu
Date: Fri, 7 Jun 2002 11:17:09 -0400
Subject: LKB microtome manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for the operations manual for an LKB Ultratome III.
Could someone contact me offline and FAX or mail us a copy? Thanks
so much.
--
David H. Hall, Ph.D.
Center for C. elegans Anatomy
Department of Neuroscience
Albert Einstein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461

www.wormatlas.org
www.aecom.yu.edu/wormem

phone 718 430-2195
fax 718 430-8821


From daemon Fri Jun 7 12:58:55 2002



From: Michael L. Boucher :      mboucher-at-tc.umn.edu
Date: Fri, 07 Jun 2002 12:52:19 -0500
Subject: Supplier of Robinson Chamber View?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Robinson Chamber View camera that we need to modify to fit a new
SEM. Does anyone know where I can contact the company or U.S. distributor?
Thanks for any replies.
Regards,
Mike
********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************








From daemon Fri Jun 7 13:26:58 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 07 Jun 2002 11:18:33 -0400
Subject: Re: Chillers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 6/6/02 1:41 PM, Anthony J. Garratt-Reed at tonygr-at-mit.edu wrote:
}
} It doesn't apply to you, but a user switching from using the public water
} supply for direct cooling will have been using a system with extremely good
} short-term temperature stability. A simple chiller does not have that -
} typically it will vary by about 2ºC over its cycle time - i.e. a few
} minutes. This will usually be very clear in the image drift! There are a
} number of methods of improving the short-term temperature stability which
} can be discussed with the chiller manufacturers.
}
Dear Tony & Lou,
Haskris, and, I'm sure, other manufacturers, makes a hot-gas bypass
attachment which provides an order of magnitude better temperature
stability. We tracked the cyclic drift from the temp changes before
ordering the bypass unit, and, when we tested for drift after the unit was
installed, the drift was negligable. BTW, the drift test suggested in the
manual--take pictures every half hour--did not catch the cyclic drift; we
had to watch for several minutes to see the problem.
Yours,
Bill Tivol



From daemon Fri Jun 7 13:53:18 2002



From: Russell Spear :      rzs-at-plantpath.wisc.edu
Date: Fri, 7 Jun 2002 13:43:28 CST
Subject: vernier scales

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a source of adhesive vernier scales that could be
attached to a microscope stage.

Thanks

Russ Spear
Russell N. Spear
Sr. Research Specialist
Dept. of Plant Pathology
Univ. of Wisconsin-Madison

RZS-at-plantpath.wisc.edu
Phone 608 263-2093
Fax 608 263-2626


From daemon Fri Jun 7 14:25:31 2002



From: Xianglin Li :      Xianglin_Li-at-student.uml.edu
Date: Fri, 07 Jun 2002 15:16:16 -0400
Subject: Asking for SEM machine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friends,

Our center is looking for a "new" SEM machine (with good condition and
resolution, within last 10 years). We want this machine could do EDX, X-
ray mapping, and with LaB6 as the filament. Of course, the cheaper, the
better.

Is anybody have a good deal for that? We will appreciate that!

Regards,

Xianglin Li

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411




From daemon Fri Jun 7 15:20:15 2002



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Fri, 07 Jun 2002 13:12:49 -0700
Subject: RE: Supplier of Robinson Chamber View?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Mike,
Try contacting Bob Rusica:


Robert Ruscica
ETP-USA
Tel:(916)797-6199
Fax:(916)797-6304
email:ruscica-at-etp-usa.com
www.etp-usa.com


-Brad


----------
From: Michael L. Boucher
Sent: Friday, June 7, 2002 10:52 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: Supplier of Robinson Chamber View?


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.


We have a Robinson Chamber View camera that we need to modify to fit
a new
SEM. Does anyone know where I can contact the company or U.S.
distributor?
Thanks for any replies.
Regards,
Mike
********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************










From daemon Fri Jun 7 22:50:17 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 07 Jun 2002 20:42:04 -0700
Subject: Glow Plug SEM analysis inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am working on a rather fun subject of a model
engine glow plug. I have put some SEM pix at

http://www.microtechnics.com/glow.htm

which will pull up a PDF catalog of SEM images.
I think that the key images are the BSE ones,
including 10, 2, and 3. What I do not know is
what is going on in these pix. I tend to see a
phase change, but the metal coil wire is at room
temperature.

If anyone has any ideas about what is seen in
these pix, please reply off-line with your comments
and attribution data. As I am planning on writing
an article about this topic, I will include relevant
feedback and will attribution specifics.

One item of interest is that this particular plug was
relatively new (about 45 minutes of running from
totally new). It faltered and was replaced by a new
plug which solved the problem. so, why does this
happen and what exactly is happening to the plugs
to degrade them? Beats me.

gary g.



From daemon Sat Jun 8 00:43:33 2002



From: Allen Sampson :      ars-at-sem.com
Date: Sat, 8 Jun 2002 00:37:24 -0700
Subject: RE: Glow Plug SEM analysis inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Any idea what the wire composition is? How about the fuel composition and
fuel/air mixture being used? What imaging was used for 8 & 9 (is 9 BSE)?
Were 8 & 9 taken at the upper coil connection point? If so, that bond
looks suspicious, unless the rest that's not shown shows a better bond.
Anything more specific on failure mode than 'faltered'?

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Friday, June 07, 2002 8:42 PM, Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am working on a rather fun subject of a model
} engine glow plug. I have put some SEM pix at
}
} http://www.microtechnics.com/glow.htm
}
} which will pull up a PDF catalog of SEM images.
} I think that the key images are the BSE ones,
} including 10, 2, and 3. What I do not know is
} what is going on in these pix. I tend to see a
} phase change, but the metal coil wire is at room
} temperature.
}
} If anyone has any ideas about what is seen in
} these pix, please reply off-line with your comments
} and attribution data. As I am planning on writing
} an article about this topic, I will include relevant
} feedback and will attribution specifics.
}
} One item of interest is that this particular plug was
} relatively new (about 45 minutes of running from
} totally new). It faltered and was replaced by a new
} plug which solved the problem. so, why does this
} happen and what exactly is happening to the plugs
} to degrade them? Beats me.
}
} gary g.
}
}
}
}



From daemon Sat Jun 8 01:04:42 2002



From: paul r hazelton, PhD :      Paul_Hazelton-at-umanitoba.ca
Date: Sat, 08 Jun 2002 01:00:49 -0500
Subject: Re: negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


jeannette

well, it certainly is an interesting protocol. but then, i know one
person who insists that staining for more than 10 seconds gives positive
staining, not negative staining, while another collaborator in a
recently submitted review suggested that he uses 5 minutes staining
time. in my experience, most staining effect is seen in the first 30-60
seconds.

what is intersting is the pH of the stain. i have seen Uranyl stains
used at low pH, but never PTA. i know that use of PTA has been reported
as low as pH 4.0, but i have only seen uncited references to this, so i
do not know what the actual studies showed. my memory is also that the
natural pH of PTA is already about 2.0, so you must not have done a lot
of adjusting. i have personally done some work at pH 6.0 and pH 8.0,
but most is at 7.0.

i know KOH can be used for pH adjustment, but it is also associated with
the degradation of virions. look at old work by Hoyle, early '60's,
where they eventually twigged that when they used KOH to neutralize PTA
the viral envelopes were degraded, but when they used NaOH the particles
did not degrade unless other treatment, which was intended to degrade
the particle, was used. the use of KOH is probably the source of the
arguement that PTA degrades the particles.

when we use NaOH we do not see particle degradation or loss of
significant loss in stain density over short time the particles are
clearly readable, with no apparant degradation over several weeks. the
biggest degradation seems to occur after being put in the scope and
exposed to vacuum and beam, and that is not major in our experience.

as far as stability of stain on the grid during prolonged storage - i
have set aside grids prepared from clinical samples as training grids
and looked at them 2-3 years later and seen clearly defined virus. the
only problem seems to occur when the grid is subjected to prolonged
exposure to beam and vacuum, which sometimes occurs because frequent
reference to the these grids by students or technicians who are in
training.

to the point, wednesday i had reason to look at a grid with subviral
particles that was prepared by a student in november. the staining was
still good, and i was able to particle count the sample. the observed
distribution of particle types, and subviral components, was consistent
with previous experiments, as was the total number of particles
observed. while several micrographs were taken for archival purpose,
they will probably never be printed. however, those negatives were
scanned through the enlarger and do look good.

in short, the problem should not be time of storage. but i would look
seriously at the use of NaOH instead of KOH for neutralization. i would
also look at higher pH, unless there is an isoelectric point issue that
you must address.

now, i must admit that i'm interested in what sara miller has to say on
the issue.

paul hazelton





From daemon Sat Jun 8 17:02:18 2002



From: Rooting :      rooting-at-hortus.com
Date: Sat, 8 Jun 2002 16:42:44 -0500
Subject: vernier scales

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a source of adhesive vernier scales that could be
attached to a microscope stage.

Thanks

Russ Spear
Russell N. Spear
Sr. Research Specialist
Dept. of Plant Pathology
Univ. of Wisconsin-Madison




McmasterCarr has some crude ones in their "adhesive backed rules" section

http://www.mcmaster.com

They are pretty inexpensive and Mcmaster does not have minimum orders and
low actual shipping chg and takes credit cards. They usually ship same day.

regards
MRK


From daemon Sat Jun 8 18:38:29 2002



From: Qian-Chun Yu, MB, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Sun, 09 Jun 2002 09:12:17 -0400
Subject: just testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is a source of small amounts of Canadian Balsam at reasonable prices.
http://studioproducts.com/catalog/balsams/canad1.html
It is from an artist supply house but it is reported to be very good quality
and suitable for slide making.

I am collecting sources of difficult to find items useful information and
posting it on a page at:
http://www.couger.com/microscope/links/gclinks.html

Any sources that any of you have for supplies would be appreciated so I can
add them to this list.

Gordon
----- Original Message -----
} From: "Rooting" {rooting-at-hortus.com}
To: "Microscope" {Microscopy-at-sparc5.microscopy.com} ; "Microscope list,"
{microscopes-at-yahoogroups.com} ; "Microscope Confocal,"
{confocal-at-listserv.acsu.buffalo.edu}
Sent: Saturday, June 08, 2002 5:40 PM


this is just to test that my message will be able to reach the community.
please disregard any information that I may put in here. thanks!

QCY



From daemon Mon Jun 10 00:16:52 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 09 Jun 2002 22:02:37 -0700
Subject: Looking for EDX detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It's becoming increasingly clear to me that I could
greatly benefit from having an EDX system on my
FESEM. The most cost advantageous, and feature-based
solution seems to be to find a surplus detector and
mate it with electronics and software from IXRF.

If you have a homeless detector (Si(Li) or other type)
that uses LN2 or cryo cooling, please contact me off-line
and let me know the details of what you have....and
cost of transfer.

I'm hoping to mate the detector to an Amray 1910FESEM.
It would use the left rear port (looking at the chamber
from the front).

gary g.



From daemon Mon Jun 10 06:16:43 2002



From: Oparowski, Joseph :      Joseph_Oparowski-at-bose.com
Date: Mon, 10 Jun 2002 07:09:00 -0400
Subject: Supplier of Robinson Chamber View?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,

You can contact the US rep at;

ETP-USA
Electron Detectors Inc.
4734 Tenbury Lane
Rocklin, CA 95677
(916) 797-6199
Bob Ruscica

Good luck.


Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313


-----Original Message-----
} From: Michael L. Boucher [mailto:mboucher-at-tc.umn.edu]
Sent: Friday, June 07, 2002 1:52 PM
To: Microscopy-at-sparc5.microscopy.com


We have a Robinson Chamber View camera that we need to modify to fit a new
SEM. Does anyone know where I can contact the company or U.S. distributor?
Thanks for any replies.
Regards,
Mike
********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************








From daemon Mon Jun 10 07:41:32 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Mon, 10 Jun 2002 07:31:40 -0500
Subject: Glow Plug SEM analysis inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary,

Allen had some good questions, but going ahead without all the answers...

Generally, the low-Z indications suggest carbon/hydrocarbon deposits which
likely occurred after the filament was at temperature. Did you do EDS? I
would suspect very high carbon, some oxygen, and a smattering of low
concentration elements. Ref: Glow 2-1, 2-10, Glow 3-12 This would not
have survived actual operational temperatures, I suspect.

Ref. Glow 3-1, 3-2, 3-3: Possible intergranular separation which is
(partially) filled with after-the-fact carbonaceous material. W wire is
typically exhibits long, fibrous like deformed grains.

In Glow 1-2, 1-3 I believe you are seeing grain channeling contrast. The
grains are more equiaxed here and I would suspect it was from an area that
was much hotter (not too hot) than the fibrous area.

In 1-4, the image suggests to me (in concert with the others) more low Z
material on the surface which did not play a role in the failure.


Woody White
McDermott Technology Inc.
McD: http://www.rdd.mcdermott.com/
Mine: http://woody.white.home.att.net


-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Friday, June 07, 2002 11:42 PM
To: MSA listserver


I am working on a rather fun subject of a model
engine glow plug. I have put some SEM pix at

http://www.microtechnics.com/glow.htm

which will pull up a PDF catalog of SEM images.
I think that the key images are the BSE ones,
including 10, 2, and 3. What I do not know is
what is going on in these pix. I tend to see a
phase change, but the metal coil wire is at room
temperature.

If anyone has any ideas about what is seen in
these pix, please reply off-line with your comments
and attribution data. As I am planning on writing
an article about this topic, I will include relevant
feedback and will attribution specifics.

One item of interest is that this particular plug was
relatively new (about 45 minutes of running from
totally new). It faltered and was replaced by a new
plug which solved the problem. so, why does this
happen and what exactly is happening to the plugs
to degrade them? Beats me.

gary g.



From daemon Mon Jun 10 07:41:53 2002



From: Susan Carbyn :      CarbynS-at-agr.gc.ca
Date: Mon, 10 Jun 2002 08:34:34 -0400
Subject: Edwards 306A Coating Instructions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there,

If anyone has a set of instructions for downward evaporation of carbon using this machine, could I have a copy? We have three very different sets of instructions, none of which are very clear. We don't use liquid nitrogen with this machine.

Please contact me off line (or fax a copy) if you can help me out.

Thanks,

Susan



Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, N.S. B4N 1J5
Canada

Phone (902) 679-5535
Fax (902) 679-2311

E-Mail: carbyns-at-em.agr.ca



From daemon Mon Jun 10 09:55:09 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Mon, 10 Jun 2002 11:38:58 -0500
Subject: Yur help re: Size of transferrin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Would any of you have a reference for the size of the
transferrin and the transferrin receptor?
Thanks for your help once again.
Rosemary

--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Mon Jun 10 11:09:16 2002



From: S Keller :      swtkeller-at-yahoo.com
Date: Mon, 10 Jun 2002 09:01:16 -0700 (PDT)
Subject: TEM: Looking for a TEM w/ asid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:
I am looking for a TEM with STEM preferably a 200 Kv
JEOL.
Thx,
Sandra Keller

__________________________________________________
Do You Yahoo!?
Yahoo! - Official partner of 2002 FIFA World Cup
http://fifaworldcup.yahoo.com


From daemon Mon Jun 10 14:06:26 2002



From: Josh Kahn :      4jbk1-at-qlink.queensu.ca
Date: Mon, 10 Jun 2002 14:57:21 -0400
Subject: LM/TEM - cellular extraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Can anyone recommend an extraction protocol to remove all cellular
material except intermediate filaments?

JBK
Read books




From daemon Mon Jun 10 15:49:25 2002



From: Don Grimes :      microtoday-at-mindspring.com
Date: Mon, 10 Jun 2002 14:56:34 -0500
Subject: Possible Virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,
Phil Oshel advises me that either my computer is infected with a virus, or
one of the people
I email is, and the virus is spoofing my address (pretending it was sent by
me/Ron Anderson, instead of the computer it really came from).
That address is microtoday-at-verizon.net and the subject line is: "Contar con
la carta de liberaci"
Not to be confused with my address, as above, or with that of Ron Anderson
(microtoday-at-attglobal.net), the new editor of Microscopy Today.
Regards to all,
Don Grimes



From daemon Mon Jun 10 17:14:54 2002



From: PHYSIOL4-at-AKAD.SUN.AC.ZA
Date: Mon, 10 Jun 2002 12:40:44 +0200
Subject: LM: need help fixing + embedding sugarcane

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Hi there

Does anyone have any experience with fixing and embedding
sugarcane stem tissue for in situ hybridization? So far we have
tried a number of protocols - including traditional fixation in 4%
paraformaldehyde, as well as prefixing and then freezing in
isopentane and cutting frozen sections - but to no avail. With
traditional chemical fixation (a protocol which worked beautifully for
one of our colleagues working on grape berries), we have to fix for 5-
7 days just to get adequate fixation, and even then parts of the
older internode tissue don't fix properly - this makes sectioning very
tricky. With cutting prefixed frozen tissue, our sections break up
very easily, and even when we do get sections, once thawed they
go into some sort of shock and develop thick black cell borders
(even in the xylem).

If anyone has any ideas, please let me know!

Thanks
Gabrielle



Gabrielle Turner
Institute for Plant Biotechnology
University of Stellenbosch
7600

Cell: 083 324 7453


From daemon Mon Jun 10 18:01:25 2002



From: David Hall :      hall-at-aecom.yu.edu
Date: Mon, 10 Jun 2002 18:54:35 -0400
Subject: lab microwave oven in Northwest Ohio

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This question is for a friend who is doing TEM in Toledo, Ohio. She
is hoping to get access to a lab nearby with an energy controlled
microwave oven (Pelco, for instance) where she could conduct
fixations from time to time.

You can direct responses to my email address. Many thanks.

Dave
--
David H. Hall, Ph.D.
Center for C. elegans Anatomy
Department of Neuroscience
Albert Einstein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461

www.wormatlas.org
www.aecom.yu.edu/wormem

phone 718 430-2195
fax 718 430-8821


From daemon Mon Jun 10 18:16:59 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 10 Jun 2002 16:10:21 -0700
Subject: RE: Glow Plug SEM analysis

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I've received several very informative responses to my original
posting--many thanks to you all.

Some responses suggested that I am going through this
exercise for income purposes.....not. See:

http://www.photoweb.net/pw_trav/RC/RC.html

Nope....I'm doing this at a loss of money but a gain in fun and
education. Glow plugs are key items in 2- and 4-stroke
RC engines. Having a plug die in the middle of a competition
aerobatic maneuver is not at all good. Having one not start
on the ground when it's my turn is also not good. I'm newly
returning to RC as a hobby. Applying SEM and new
analytical technology to RC is very enjoyable.

It turns out that the glow plug wire is made of Platinum
and Rhodium. The Pt acts as a catalyst when introduced
with methanol. I don't know if there is a difference between
the 2- and 4-stroke plug wires. The veteran RC enthusiasts
are very knowledgeable about many aspects of this topic.

I put additional images at the site.

http://www.microtechnics.com/glow.htm


These added images are
of new 4-stroke glow plugs. They do not show the dramatic
grain boundaries seen in the failed 2-stroke plug. So, do
the boundaries show up after use, are they only seen in
2-stroke plugs, or what? Perhaps the 2-strokes are an
alloy whereas the 4-strokes are just Pt. More questions
and unknowns.....sigh.

But the difference in wire quality between the two 4-stroke
engine plugs is, I believe, significant. I have not used
these plugs yet. Now I will have to keep track of each
plug's experiences in order to make a valid experiment.
I had not thought about this much complication...

With Z of 78/Pt and 45/Rh, I ought to be able to see
the grain boundaries with BSE. I cannot do this with
a new plug. Therefore, as Warren and Woody have suggested,
there is the possibility of visualizing the effects of high
temperature exposure. I have seen this in ICs at Al/Si
alloy areas. But this was fundamentally when the
wafers were sintered too long or reflow was done
improperly.

One thing is clear however, there is marvelous depth of
field and clarity in looking down the coiled wire in the plug.
The SEM can do this with ease. LM has a very tough
time attempting this feat. The

This is becoming quite involved. Not sure how useful this is
other than to satisfy my own curiosity. There is perhaps
a way to electrically test new plugs, based on what I have
seen as clear defects in new plugs. However, considering
that a new plug costs about $7, it is probably not economical
to worry about pre-qualifying plugs! But making an assessment
of the quality of each brand and type does make sense.

I do wish that I had x-ray capability. I'm working on that.
There have been several good responses to my listserver posting
about that--thanks. I hope that an EDX system is not like
a boat--a place to dump lots of money. At least a dewar
is small and could be used as a boat anchor in desperation.

gary g.




At 05:31 AM 6/10/2002, you wrote:

} Hi Gary,
}
} Allen had some good questions, but going ahead without all the answers...
}
} Generally, the low-Z indications suggest carbon/hydrocarbon deposits which
} likely occurred after the filament was at temperature. Did you do EDS? I
} would suspect very high carbon, some oxygen, and a smattering of low
} concentration elements. Ref: Glow 2-1, 2-10, Glow 3-12 This would not
} have survived actual operational temperatures, I suspect.
}
} Ref. Glow 3-1, 3-2, 3-3: Possible intergranular separation which is
} (partially) filled with after-the-fact carbonaceous material. W wire is
} typically exhibits long, fibrous like deformed grains.
}
} In Glow 1-2, 1-3 I believe you are seeing grain channeling contrast. The
} grains are more equiaxed here and I would suspect it was from an area that
} was much hotter (not too hot) than the fibrous area.
}
} In 1-4, the image suggests to me (in concert with the others) more low Z
} material on the surface which did not play a role in the failure.
}
}
} Woody White
} McDermott Technology Inc.
} McD: http://www.rdd.mcdermott.com/
} Mine: http://woody.white.home.att.net
}
}
} -----Original Message-----
} } From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: Friday, June 07, 2002 11:42 PM
} To: MSA listserver
} Subject: Glow Plug SEM analysis inquiry
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jun 11 03:20:11 2002



From: Anaspec :      anaspec-at-icon.co.za
Date: Tue, 11 Jun 2002 10:05:15 +0200
Subject: ICEM 15 1 July registration date.

Contents Retrieved from Microscopy Listserver Archives
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Hi all

A quick update from South Africa and the organizing of ICEM15 for 1-6
September 2002.
The last chance to still save a bit on early registration is coming up fast.
1 July being the cut-off date. Registration is available online by visiting
the new revamped website. www.icem15.com

As the scientific programme has now been "finalised" there are some numbers
on the conference, available via the same website. Given all the events over
the past two years, the numbers of abstracts submitted is well over our
expectations.
The programme will be put onto the website once all last minute changes have
been made. In the mean time there is an indication of some of the topics of
interest.

The trade exhibition looks good as most stands are sold. As the trade have
been asking who and how many people will be there, we have added a page
showing the abstracts by country. Interesting reading!

Thanks for your time and hope to see you all at ICEM15 in Durban, South
Africa.

Luc Harmsen
Marketing ICEM15

www.icem15.com



ANASPEC South Africa www.anaspec.co.za
Tel: +27 11 794 8340
Fax: +27 11 794 8349
P.O. Box 2561
Honeydew 2040
Gauteng, South Africa




From daemon Tue Jun 11 08:36:39 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 11 Jun 2002 09:22:33 -0400
Subject: Re: Yur help re: Size of transferrin

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At 11:38 AM -0500 6/10/02, Rosemary Walsh wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Tue Jun 11 13:37:39 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 11 Jun 2002 13:27:58 -0500
Subject: Core Facility managment

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Fellow microscopists:
This year we will discuss equipment maintenance during the Core Facility Management session at M&M2002. The session will be on Wed morning, Aug. 7 (although the program may say Wed afternoon - a mistake that hopefully was corrected). The program is at the end of this E-mail.

A number of the facilitators have asked for direction as to their comments. I would appreciate your sending specific questions or points of interest to me. Please indicate who you would like to address your issues. I will summarize the response and send the information on to the presenters. This should help make for a more productive session.

The NSF representative may not be able to make it to the meeting. However, if I have specific questions for NSF related to equipment purchase and their policies on funding of service contracts, I will try to get the information and present it at the session. Additional discussion and questions generated at the meeting will be clarified and sent out via the list.

We again hope to tape the discussion for later publication...providing I get the time to do the transcription.

See you in Quebec!
Debby

Maintaining Major Equipment in a Core Microscopy Facility

8:30: Servicing by Original Equipment manufacturers: the structure, function & considerations for assembling & operating a major service organization
JEOL: Patrick McGinley
National Service Manager
FEI: Mike Kearney
Director of Service
Hitachi: Greg Rigby
Director of Service

9:15: Servicing EMs by On-site staff: Insight into the Do-it-yourself Approach.
John Wheatley, Arizona State University
Owen Mills, Michigan Technological University

10:00: Break

10:30: Using 3rd Party Service Organizations for Major Equipment Maintenance
Art McCanna
Service Manager
Materials Analytical Services

11:00: Policies Regarding Allowable Costs for Equipment Maintenance on National Science Foundation (NSF) Awards

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907





From daemon Wed Jun 12 07:44:23 2002



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Wed, 12 Jun 2002 08:27:51 -0400
Subject: Salary Survey for Microscopists

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Two or three years ago, "Microscopy Today" conducted a salary survey for
microscopists based on years of experience, education and region. Does
anyone know if that was archived anywhere? Thank you!

Lesley Bechtold



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Wed Jun 12 09:31:38 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Wed, 12 Jun 2002 10:23:15 -0400
Subject: Roughness from IPP scan

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Greetings Microscopists:

I would like to measure roughness from a microtomed cross section of a
surface replica. We're using Image Pro Plus to trace the interface
generating a representation of the surface profile. Is there some freeware /
software available to get from the trace generated by IPP to some
quantification like Ra???

Thanks,

Russ Gillmeister
Microscopy
Xerox Corp.
Webster, NY 14580




From daemon Wed Jun 12 10:51:27 2002



From: woxberry-at-downstate.edu
Date: Tue, 11 Jun 2002 22:21:15 -0500
Subject: question on cryoultramicrotomy equipment

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers,

I am trying to get an old FC-4 cryo unit up and running to do some
cryosectioning on my Ultracut E. It seems to be missing some parts,
specifically the LN2 transfer hose from the dewar to the unit and a male
threaded transfer tool to transfer the specimen holder into the
chamber. I only
have one knife holder .

Does anyone have an FC-4 gathering dust somewhere who might want to find it a
new home? Or at least the LN2 transfer hose? I found one source for
a hose but
they want over $600 ! I don't want to buy a hose and find out that the unit
doesn't work.
Thank you all.

Bill Oxberry
Core Microscopy Lab
Dept. of Path.
Downstate Medical Center
Brooklyn, NY
woxberry-at-downstate.edu
7182704472


From daemon Wed Jun 12 13:40:12 2002



From: Thearith H. Ung :      tung-at-qdots.com
Date: Wed, 12 Jun 2002 11:28:18 -0700
Subject: RE: Urylacetate

Contents Retrieved from Microscopy Listserver Archives
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Would anyone be interested in having a solution of urylacetate (25g)? We
have a bottle that has not been opened since it was purchased. We would be
happy to ship it out to anyone interested. Please let me know as soon as
possible if you would to have it.

Regards,
Thearith
_________________

Thearith Ung

Quantum Dot Corporation
26118 Research Road
Hayward, CA 94545, USA
Tel: 510-887-8775 (Ext 4125)
Fax: 510-783-9729
Email: tung-at-qdots.com



From daemon Wed Jun 12 13:58:41 2002



From: Eric Anderson :      anderson_e-at-southernct.edu
Date: Wed, 12 Jun 2002 14:52:29 -0400
Subject: Re: Philips EM400 Matching Mains Transformer

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Greetings Vitaly,

Sorry to trouble you again, but I think I may have goofed. When I told our
campus electricians to install a 220V service for the EM400 (before I had the
manuals), they naturally gave us two hots and a neutral. From each hot to
neutral is about 118V. From hot to hot is only 205V. When I asked the
microscopy group for help with my 237V, I was merely adding the two separate
measurements with respect to neutral, and worse, I had not considered that
Philips asks for a 3-wire hook up consisting of 220V/neutral/ground, not
110/110/neutral.

Since the EM400 has a power spec of 8 kVA and the Buck & Boost only 1 kVA, I
called the transformer manufacturer Square D, and they say I need a custom
built $2000 isolation transformer to get the mains set up properly (without the
Buck&Boost), or else a $1200 stock gizmo to use with the Buck&Boost. I already
have the Buck & Boost, but sadly, our budget is virtually exhausted. Our
electrician thinks we should try using the two hots in place of the
220/neutral, but I'm very reluctant. Do you operate an EM400, and how is yours
powered?

Thanks so much,
-Eric
---------------------------
Eric Anderson
SCSU Physics Adjunct
203-392-6455
anderson_e-at-southernct.edu
---------------------------
Vitaly Feingold wrote:

} Eric,
}
} 237V is a bit too high. It must be between 208V and 220V. The simplest and
} economical way to power up your TEM is to use Buck and Boost transformer
} connected as autotransformer. Philips matching unit originally supplied with
} EM400, was also an autotransformer. Order from Grainger, www.grainger.com ,
} many locations everywhere, stock # 1H270, $174. This one will reduce your
} line voltage by 24V, making it 213V to 211V (see below), which is perfect.
}
} Notes.
} 1) Make sure that grounding is correct and safe.
} 2)This unit can be connected in 4 different ways, for increasing or reducing
} the line voltage by 12V or 24V, and is shipped with 1 page manual. Read it.
} 3) The above part number is given for your particular (line voltage) case.
} Others may require a transformer with different part number.
} 4) Transformer will not stabilize the line voltage, only change it.
} 5) I assume that you measured line voltage with no load connected- the
} actual voltage may drop about 1V or 2V when you connect the TEM.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
} ----- Original Message -----
} From: Eric Anderson {anderson_e-at-southernct.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, June 04, 2002 12:53 PM
} Subject: Re: Philips EM400 Matching Mains Transformer
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } } Greetings All!
} } }
} } } We are in the process of setting up a second-hand EM400 acquired
} } } recently, and have found that the mains matching transformer was not
} } } included. Our raw supply main is 237 volts/60Hz (118V on one leg,
} } } 119V on the other, stability unknown), and I'm thinking this is not
} } } close enough to the specified 220V to go without the transformer. Any
} } } ideas? If we do need some line conditioning, can anyone recommend a
} } } particular device, or source for the original Philips transformer?
} } }
} } } Many thanks for any tips!
} } } -Eric



From daemon Wed Jun 12 14:43:26 2002



From: Pierre-M. Charest :      pcharest-at-rsvs.ulaval.ca
Date: Wed, 12 Jun 2002 15:32:30 -0400
Subject: M&M 2002 - Quebec City

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Dear Colleagues Microscopists

The count down has started. In less than two months, Microscopy &
Microanalysis 2002 will begin in Quebec City, 4-8 August 2002. The
deadline for pre-registration is July 6. You can register on-line at
the following URL address:

http://www.microscopy.com/MSAMeetings/MMMeeting.html

More that 800 papers and posters will be presented at this meeting.
The scientific and social program will certainly please you. This
meeting could also be a unique opportunity to visit the French cradle
of North America. On August 7 the "Festival de la Nouvelle France"
will be launched, you will be witness of the life style of the early
French and English citizens who colonized our land from the XVIIth
Century. The old city will be crowded with people dressed like three
centuries ago.

If you visit Quebec with your family or friends, do not miss the
Whale Watching Cruise organized either on Wednesday August 7 or on
Friday August 9. You can make reservation on-line on the web site of
the Local Arrangement Committee where several tips are also offered
for your travel to Quebec, your lodging and your sightseeing
activities.

http://msc.rsvs.ulaval.ca/2002/2002.html

All members of the Local Arrangement Committee are eager to
facilitate your stay in our home and we hope we'll have the pleasure
to seeing you here.

AU REVOIR


PIERRE, CHAIR
LOCAL ARRANGEMENT COMMITTEE



From daemon Wed Jun 12 15:02:31 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 12 Jun 2002 14:56:02 -0500
Subject: SEM/ Cryo unit sputter coater

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Hi all,

We have an Emitech cryopreparation unit associated with our Hitachi S4700 FESEM. The cryo unit's sputter coater is currently outfitted with a gold target, but we'd like to try platinum for a finer grain coating. The current target appears to be a thick sheet of gold, wrapped around the support and glued in place.

My main question: is there any reason we couldn't purchase a similar, malleable sheet of platinum and just wrap it around the gold for when we want to coat with Pt? Seems to me like it would work, but I like to check these things with the experts first.

Also, if anybody has any wisdom to share on avoiding getting ice on samples during cryo runs, I'd love to hear it. We often (usually) get ice during transfers and need to sublime off the moisture before coating, otherwise the coating forms quasi-permanent ice "casts" that render the sample unusable. We've replaced/checked all the o-rings we can reach, so any special hints are very welcome.

Finally, has anyone ever tried mixing colloidal carbon (like SEM mounting carbon) with the polyvinyl alcohol cryo mounting medium in order to improve conductivity? We're going to give it a whirl, but it would be interesting to hear if others have tried it.

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Wed Jun 12 20:10:21 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 12 Jun 2002 21:04:43 -0700
Subject: Re: SEM/ Cryo unit sputter coater

Contents Retrieved from Microscopy Listserver Archives
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} HI Randy,

I use an Oxford (now GATAN) C1500C system and have some experience
imaging and analyzing cells grown in inserts, tissues, polymers,
emulsions and foods. When possible I prefer unfixed tissue or fixed,
cryoprotected cells.

} Also, if anybody has any wisdom to share on avoiding getting ice on
} samples during cryo runs, I'd love to hear it. We often (usually) get ice
} during transfers and need to sublime off the moisture before coating,
} otherwise the coating forms quasi-permanent ice "casts" that render the
} sample unusable. We've replaced/checked all the o-rings we can reach, so
} any special hints are very welcome.



In order to insure some success in removing surface ice, I blot excess
fluid from a 2mm sample, plunge into LN in a transport dewar located as
close to the SEM sample prep chamber as it possible, cover and quickly
press the N admit to open the chamber. After inserting the transfer holder
onto the prep cryostage (-170C), I pull a vaccum, wait 2 min., fracture if
needed, transfer onto the SEM coldstage, close the ball valve and
immediately begin heating the stage.I prefer to do the transfer this way
rather than into the LN slush where I run the risk of heavy ice
contamination. Etching or sublimation can take 15 min for plant tissues,
biopolymers to 45 minutes for emulsions and frozen foam foods (yogurt, ice
cream).

If your sample size is large 5-10 mm, I attach to the sample holder with
graphite/OCT drop and immediately insert onto sample prep stage (-170C) and
proceed as outlined above---I've also modified this by progressively
lowering temp using ice, dry-ice, LN plunge.
One adaptation for dry-ice use is to cut out a hole in the side of a
styrofoam container so that the transfer rod can be inserted into dry ice
vapor. It is the only way I to work with frozen samples.

It's is important to minimize the sample size--I use gold planchets from an
old Balzers FF/FE system -- they have a tiny depression which holds 10
uls. This sits in a brass holder with an aluminum cap which holds the
planchet in place. I invert a second and transfer to the cryo-prep stage,
wait two minutes, fracture by touching the pick to the top planchet and
proceeding as above.
Another option is to use brass rivets--the machine shop made 10 mm brass
stubs with holes drilled for three rivets--I either drop liquid emulsion or
scoop ice cream into them, cover with an inverted- cooled rivet, insert
onto the cryo prep stage, pull a vacuum, tip the top rivet so that it
fractures, insert into the SEM cryo-stage to begin sublimation and proceed
as above.

It is imperative for us to schedule cryo-SEM work during lower humidity
times. July and August are difficult times. If I have to work then, I run
a dehumidifier but it is still problematic.


} Finally, has anyone ever tried mixing colloidal carbon (like SEM mounting
} carbon) with the polyvinyl alcohol cryo mounting medium in order to
} improve conductivity? We're going to give it a whirl, but it would be
} interesting to hear if others have tried it.

I routinely mix a fresh batch of OCT cryo-mountant with a couple of drops
of colloidal graphite, place a small drop onto the brass mount, add a tiny
wedge of filter paper to the drop along with the sample. I use the filter
paper as a monitor for the sublimation / etch time--it has worked very well
with pieces of tissue, polymers. It is important to make this fresh
conductive adhesive, keep it in a one ml microfuge tube and apply with a
wooden pick or with a disposable 1 cc syringe.

I wish you success----ice can be maddening
Rosemary

Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212



From daemon Thu Jun 13 01:46:35 2002



From: DrJohnRuss-at-aol.com
Date: Thu, 13 Jun 2002 02:34:49 -0400
Subject: Re: Roughness from IPP scan

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In a message dated Wed, 12 Jun 2002 10:43:57 AM Eastern Daylight Time, RGillmeister-at-crt.xerox.com writes:

} I would like to measure roughness from a microtomed cross section of a
} surface replica. We're using Image Pro Plus to trace the interface
} generating a representation of the surface profile. Is there some freeware /
} software available to get from the trace generated by IPP
} to some
} quantification like Ra???

The Fovea Pro (ReindeerGraphics.com) plugins run in Image Pro Plus version 4.5 and include this function. Besides Ra you also get Rq and some other standard profile roughness values.





From daemon Thu Jun 13 03:14:05 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 13 Jun 2002 09:06:57 +0100
Subject: Re: SEM/ Cryo unit sputter coater

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Randy
It is possible to make up your own targets. If you buy discs of
precious metal sheet from a reputable dealer you will usually save a
considerable amount of money compared with the sputter manufacturer's
prices. And you can specify target thickness. But make sure the metal
purity is very high.
Although you could wrap platinum round the gold for a temporary fix,
it would probably be a better plan to buy a spare target base and make
that up with your platinum sheet. It is important to ensure secure
electrical connection between the base and the metal foil. The easiest
way to do this is to glue the foil to the base with silver-loaded
epoxy. SPI, TAAB, Agar etc. can usually supply this.

Penny- (or dime-) pinchers tip: Sputter coaters often burn through
their targets in a narrow ring leaving a huge amount of perfectly
good metal in the centre and round the edges. When this happens with
your second target you can use the gold saved from the first to patch
the holes.

} We have an Emitech cryopreparation unit associated with our Hitachi
S4700 FESEM. The cryo unit's sputter coater is currently outfitted
with a gold target, but we'd like to try platinum for a finer grain
coating. The current target appears to be a thick sheet of gold,
wrapped around the support and glued in place.
}
} My main question: is there any reason we couldn't purchase a
similar, malleable sheet of platinum and just wrap it around the gold
for when we want to coat with Pt? Seems to me like it would work, but
I like to check these things with the experts first.

It is virtually impossible to avoid some contamination of exposed
surfaces with ice during cryofixation and the initial transfer stage.
The first source is particulate ice in the nitrogen slush, condensed
from the atmosphere when the nitrogen is poured into the dewar.
Usually this ice is in the form of flocculent particles that are
easily sublimed at -100oC or above. Ice condensed on the cold specimen
directly from the moist atmosphere will also be finely granular, and
easily sublimed. This type of ice is what you will normally observe if
there is a leak in your transfer system seals. If you are seeing
encasing films or sheets of ice covering the specimen there was
probably a film of liquid water covering the specimen before it was
cryo-fixed. This is a major problem when examining the surfaces of
cultured cells, for example. The only way I can think of to deal with
this if you want to observe fully or partially hydrated material is to
blot the surplus water off thoroughly immediately before fixation, and
then etch some ice off. The problem with this is that the etch times
will be quite long, and since the film will be of very uneven
thickness very variable amounts of freeze-drying will occur in the
specimen.

} Also, if anybody has any wisdom to share on avoiding getting ice on
samples during cryo runs, I'd love to hear it. We often (usually) get
ice during transfers and need to sublime off the moisture before
coating, otherwise the coating forms quasi-permanent ice "casts" that
render the sample unusable. We've replaced/checked all the o-rings we
can reach, so any special hints are very welcome.

Aqueous carbon dag is quite effective as a mountant on its own or when
mixed with Tissue-Tek. Dirty though! I have used a 50:50 mixture, but
the effective proportions will depend on the solids content of your
carbon dag preparation. You could try measuring the resistivity of the
frozen mixtures. I don't recommend using SEM mounting carbon dag of
the Leit C type which uses an organic solvent.

Chris
} Finally, has anyone ever tried mixing colloidal carbon (like SEM
mounting carbon) with the polyvinyl alcohol cryo mounting medium in
order to improve conductivity? We're going to give it a whirl, but it
would be interesting to hear if others have tried it.
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}




From daemon Thu Jun 13 08:29:22 2002



From: TCLEEG-at-tsmc.com.tw (by way of MicroscopyListserver)
Date: Thu, 13 Jun 2002 08:10:49 -0500
Subject: Senior TEM analyst position at tsmc

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}
}
}
} Senior TEM analyst
}
} Taiwan Semiconductor Manufacturing Company (TSMC) is seeking
} qualified candidates for the position of Senior Failure Analysis
} Engineer for the Process Failure Analysis Department located in
} Hsin-Chu, Taiwan. The position involves TEM analysis of
} reliability failures and customer returns using tool such as
} JEOL2010F, JEOL 2000 EX and FIB DB835 etc. The successful
applicant
} will also be responsible for developing solutions to technical
} problems and issues in the failure analysis group, including the
} analysis and interpretation of results to resolve
} product/process and customer problems, independent investigative
} work, new
} analytical technique development and presentation of results in
} customer and technical meetings.
}
} Applicant Qualifications:
} - High level of knowledge and hands-on experience in TEM analysis.
} - Ability to conduct independent laboratory investigations.
} - Good oral and written communication skills as required in
} preparing reports on TEM results and problem investigation.
} - Experience with semiconductor materials analysis and sample
} preparation is a plus.
}
} Interested individuals should send a detailed cover letter and
} resume to: Tan-Chen Lee, Process Failure Analysis Department,
} Taiwan Semiconductor Manufacturing Co., 25 Li-Hsin Rd.
} Science-Based Industrial Park Hsin-Chu, Taiwan 300 or via
} e-mail:tcleeg-at-tsmc.com.tw
}
}
}
} Best regards,
}
} Tan-Chen Lee ???
} Process Failure Analysis Department
} Taiwan Semiconductor Manufacturing Company, Ltd.
} 25, Li-Hsin Rd, Science-Based Industrial Park
} Hsin-Chu, Taiwan, R.O.C.
} tcleeg-at-tsmc.com.tw
}
}


From daemon Thu Jun 13 08:50:36 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 13 Jun 2002 08:44:12 -0500
Subject: Re: SEM/ Cryo unit sputter coater

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From: Debby Sherman :      dsherman-at-purdue.edu
Date: 13 Jun 2002 08:44:12 -0500
Subject: Re: SEM/ Cryo unit sputter coater

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Randy,
We have mixed carbon with Tissue Tec OCT cryoadhesive since the early 80's for cryo-SEM. Formulation we use is:
0.4g carbon powder (can be obtained from waste from sharpening carbon rods used for carbon coating)
2gm 95% ETOH
10gm OCT compound
Mix well and let sit for a few days prior to using.

Also, I use an 18 year old Hexland/Oxford/Gatan system for plunge freezing/coating etc. It is lower tech than today's versions but still does imazingly well. We have minimum problem with ice on samples accumulated during transfer. The only time I sublimate is if I have to fracture a sample and remove some of the water from the fracture surface to better reveal the structure. Now this is with a standard tungsten-filament instrument so maybe we are not seeing the water. However, it is not visible at magnifications up to ~5000x, which is about as high as we can go with biological samples before excessive noise swamps the signal and we get into the "empty magnification" problems.
I am not familiar with the EMItech accessory so cannot comment on possible reasons or solutions for this ice problem but assume the company may have some suggestions. Also, perhaps they can manufacture a target for you from another metal. However, wouldn't it require different voltage and possibly vacuum conditions to sputter platinum instead of gold? I would appreciate hearing if you manage to do this as it will be of value to many of use who do cryo and hope to do it on FESEM's in the future.

Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907


On Wednesday, June 12, 2002 2:56 PM, Tindall, Randy D. {TindallR-at-missouri.edu} wrote:
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From daemon Thu Jun 13 09:15:14 2002



From: Microscopy Today :      microtoday-at-attglobal.net
Date: Thu, 13 Jun 2002 10:08:52 -0400
Subject: Salary Survey for Microscopists

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Reply-To: {microtoday-at-attglobal.net}


The Microscopy Today archives for the last five years are on the MT
website, www.microscopy-today.com
I have copies of most of the old issues.

Ron Anderson, MT Editor

-----Original Message-----
} From: Lesley S. Bechtold [mailto:lsb-at-jax.org]
Sent: Wednesday, June 12, 2002 8:28 AM
To: microscopy-at-sparc5.microscopy.com


Two or three years ago, "Microscopy Today" conducted a salary survey for

microscopists based on years of experience, education and region. Does
anyone know if that was archived anywhere? Thank you!

Lesley Bechtold



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191





From daemon Thu Jun 13 12:14:03 2002



From: eld26-at-cornell.edu
Date: Thu, 13 Jun 2002 13:04:13 -0400 (EDT)
Subject: Polishing trabecular bone for AFM/nanoindentation

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Listers,

I have some samples of cancellous bone embedded in both spurrs and PMMA,
and I plan to make small indentations (1-2 microns) within a single
lamella. To do this, though, I need a relatively smooth (~ {10 nm rms
roughness on a 20 um scan) and uniform surface.

My current surface prep procedure of manually grinding on SiC paper
through 1200 grit and then polishing with Al2O3 on hard nylon cloth down
to 0.05 um particle size results in samples that are generally too rough
(~70-120 nm rms roughness on a 20 um scan), and the surface finish is
highly variable from point to point within a sample.

Does anyone have suggestions for improving the surface finish?
Unfortunately, I don't have access to automatic polishing equipment, and
I'm wary of chemical treatments that may alter the surface properties I'm
trying to measure.

thanks very much,


Eve Donnelly
Sibley School of Mechanical & Aerospace Engineering
Cornell University
Ithaca, NY 14850
tel: (607)255-3582




From daemon Thu Jun 13 14:12:37 2002



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Thu, 13 Jun 2002 15:04:32 -0400
Subject: Uranyl Formate

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Hi All,

I'm posting this for a microscopist not on the listserver. They
are testing uranyl formate as a negative stain without much success. Does
anyone have any tips or methods for getting this to work? Thanks!

Lesley Bechtold

Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Thu Jun 13 15:34:47 2002



From: hseyan :      hseyan-at-is.dal.ca
Date: Thu, 13 Jun 2002 17:26:18 -0300
Subject: Double immuno staining for TEM

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Hi,
I would like to know if there is anybody who has successfully managed to
immunostain nerve cells in brain tissue labeled with Cholera toxin (Ctb) using
antibodies against CTB,developing with TMB method, and then follow it with
antibody against GABA using a goldconjugated secondary. The preparation is to
be viewed using a TEM. Please advise.

harjit Seyan
hseyan-at-is.dal.ca




From daemon Thu Jun 13 17:06:53 2002



From: Lewis Melissa A :      LEWISMA-at-medicine.ufl.edu (by way of
Date: Thu, 13 Jun 2002 16:55:44 -0500
Subject: glass knives

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I am having problems with my water level not staying on the knife
edge. I am cutting Lowicryl, so the water level has to be very low.
This problem has occurred with the last two batches (10-15) of knives
that I have made. I have tried everything I know with no success.
Does anyone have any suggestions. Please:)


From daemon Thu Jun 13 17:06:53 2002



From: Robert Johnson :      spore1-at-worldnet.att.net (by way of
Date: Thu, 13 Jun 2002 16:56:16 -0500
Subject: Greetings EM in D.C.?

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Dear Friends,

I am an early career Biologist and photographer who is new to the
Washington, D.C. area and interested very much in working in the field of
electron microscopy and wish to make myself available to labs needed extra
help. Should anyone be able to provide advice or contact information of
parties willing to meet with me and tour their facility, I would be very
grateful.


-Robert Johnson

(202) 265-2264

spore1-at-worldnet.att.net


From daemon Thu Jun 13 18:18:07 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 13 Jun 2002 16:11:01 -0700
Subject: Re: Uranyl Formate

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Dear Lesley

Use uranyl formate fresh (1-2 h after preparation) and avoid exposure to
the direct light (alumina foil wrap when dissolved, diffuse room light when
prepare samples - no direct high intensity light). I am using 1% UF
dissolved in ultrapure H2O for 30-40 min with gentle mixing in the
dark. Staining time 1-2 min. The quality of staining depends from the
support film (I am using 1.5 nm thick carbon film), sample
purity/concentration. In most cases I got staining quality better that
with UA. UF in general produces fine granularity and looks less contrast
under the microscope, but it went fine on the film.

I hope it help. Sergey

At 12:04 PM 6/13/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jun 13 18:51:36 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Fri, 14 Jun 2002 09:48:23 +1000
Subject: RE: SEM/ Cryo unit sputter coater

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Hi Randy,

Yes the slow-freeze process is very simple, exactly as you say - we just
attach the plant material to any type of stub with carbon dag and freeze on
the cold preparation stage in the coating/prep chamber. This almost
completely eliminates any frost, in fact we rarely check for frost. If
it's a very precious specimen we check the surface at 2-3 kV before
coating. The only problem we have is if the plant tissue is broken and
exudes cell contents before freezing. If this is going to be a problem we
just work very quickly and mop up gently - for example, if we want to look
at the innards of a flower after removing some of the sepals and petals, or
if we want to look at a stem or root cross-section. We also do Arabidopsis
siliques like this to look at ovule size and morphology in various mutants
- slit in half, glue down halves onto stub, freeze, coat, observe. The
systematists/taxonomists in the Herbarium also look at all their fresh
material this way. Another group working on the soil-plant interface look
at roots in soil in this way too. Yes, we put dirt in our SEM!

This is a joint facility with the Entomology Division of CSIRO, and they do
the same for their fresh insects. With small insects, they knock them out
with CO2 I think, line up lots of them on a large stub, freeze on cold
stage of prep chamber, coat, and spend the rest of the day taking photos.

One advantage we have is that Canberra is quite dry, rarely gets above 80%
humidity in winter. Summer can be down to 20%.

With already frozen tissue then we routinely sublime off the frost by
raising the stage temperature to -90 while observing the tissue at low kV.
We drop the temperature just before all the frost has gone - have to watch
quite closely not to etch cryo-planed specimens too much. This is only for
tissue that has been frozen in the field and sent in for analysis, or for
tissue that has been cryo-planed for X-ray analysis. We attach this with
TissueTek or Leit-C. The tissue for cryo-planing is inserted into
home-made chucks that go into the cryomicrotome (home-made - by our machine
shop - is much cheaper!). Then for observation two chucks are inserted
into a specially made stub to fit onto the cryostage.

A final comment re Pt or other coating, for our Oxford 1500 CT cryochamber
we have two spare target bases (cf. comment from Chris Jeffree), one for
carbon rope, the other for W wire. As I mentioned before, for Al coating
we wrap pure Al sheet around the W wire (40 amp double strand). Al is from
Alfa Aeser, who have a vast range of pure metal bits and pieces (no
commercial interest........). You have to find the right Ar level, etc.
for good Al coating - by trial and error. But once this is sorted out,
it's quite routine. The expert on cryoplaning and Al (and Cr) coating for
X-ray analysis in Canberra is Cheng Huang, who has published quite a few
papers using these methods.

We get all our gold targets made by a local jeweller - we get them made
thicker than normal (0.6mm), and we take back the bits from worn out
targets so he can remelt this and incorporate into the new target. We've
found this to be much cheaper than getting the gold from any other source
and it only takes a day or so. I guess because any other gold targets have
to be imported, it makes a big difference here in Oz. And the jeweller has
some gold-coated insects on display in his shop....

hope this is of some use!
cheers,
Rosemary

} Hi Rosemary,
}
} Thanks for the information! I'm a little unclear about your slow-freeze
} process, though. Could you give me a little more detail on how you freeze
} the sample in the transfer chamber. On our unit, there is a freezing
} chamber for the LN2 cup, and the transfer chamber is just a holder that
} can be pumped to a vacuum to move the sample from the freezing chamber, to
} the coating/preparation chamber and then to the SEM. Do you freeze the
} sample by simply setting on the LN2-cooled preparation stage?
}
} Thanks again.
}
} Randy
}
} -----Original Message-----
} From: Rosemary White [mailto:rosemary.white-at-csiro.au]
} Sent: Thursday, June 13, 2002 12:46 AM
} To: Tindall, Randy D.
} Subject: Re: SEM/ Cryo unit sputter coater
}
}
} Hi Randy,
}
} Can't comment on Pt, but for Al coating we do exactly that - wrap a very
} thin sheet of Al around a W electrode for sputtering frozen plant material
} before X-ray analysis.
}
} Re. icing up - I guess this is transfer from freezer to cryochamber(?),
} have you ever tried "slow-freezing"? We do this all the time for plant
} material - just stick the material onto the cryo-stub with Tissuetek or
} that liquid carbon goop or whatever, then put into transfer chamber, and
} freeze. Then check for ice, usually the specimen is fine, then withdraw to
} chamber again, coat, and observe. Works for robust things, but I guess
} would not work for some animal tissues.
}
} cheers,
} Rosemary
}
} }
} }


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph. 61- 2 6246 5475 or
mob. 61- 0402 835 973
rosemary.white-at-csiro.au




From daemon Thu Jun 13 22:55:28 2002



From: Sampath, Srinidhi (CORP, GEITC) :      Srinidhi.Sampath-at-geind.ge.com
Date: Fri, 14 Jun 2002 09:17:02 +0530
Subject: Characterizing inhomogeneous particle distributions...

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I am looking for techniques, protocols and programs which will give a
quantitative measure of particle distribution in a matrix. Emphasis is on
inhomogeneous distributions and how to characterize them.
I would appreciate any information on this. You could also reply to my
e-mail address.

Thanks,

S. Srinidhi,
Materials Scientist
John F. Welch Technology Center
_______________________________________
Materials Research Laboratory
Sy #152, Export Promotion Industrial Park Phase - 2
Hoodi Village, Whitefield Road,
Bangalore - 560066.
Phone: +91-80-8412050 - 69 x: 2562
Fax: +91-80-8412111
e-mail: srinidhi.sampath-at-geind.ge.com
Dialcom: 8*901 2562


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From daemon Thu Jun 13 23:29:21 2002



From: jerzy.gazda-at-amd.com
Date: Thu, 13 Jun 2002 23:22:48 -0500
Subject: Bacteria on semiconductor

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Experts,

as a materials scientist I don't have too much exposure to bio-elemental analysis. Could someone in Bio-fields help out. What elements should I expect to find in biological residue? I am evaluating suspected bacterial deposits (contamination) and finding O, C, and Ca. Would there be any other elements present after the residue was encapsulated in high temperature deposited silicon oxide?

Thank you in advance.

Warmest Regards from Texas.

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From daemon Fri Jun 14 02:54:01 2002



From: heiko.stegmann-at-amd.com
Date: Fri, 14 Jun 2002 09:48:19 +0200
Subject: Re: Bacteria on semiconductor

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Rosemary
While I concede the slow-freeze method works on some specimens - it
can as you indicate be
particularly successful with leaves or small insects that are covered
with a cuticle -
such recidivism should only be practised by consenting adults fully
aware of the implications, which are
a) wet stuff (possibly including organic solvents) has to be
introduced to the high vacuum of the prep chamber thus contaminating
cold stage and anticontaminator of the prep chamber
b) uncontrolled evaporation from the surface of the un-fixed specimen
c) thus specimens without the benefit of a protective covering (such
as cultured cells) will show variable amounts of drying artefacts.
I suppose the frank reality is that all etching / drying procedures
result in artefacts, but there is a comforting illusion of control if
you can get the same artefacts with reasonable reproducibility!

On the topic of gold for targets, I was not recommending jewellers as
a source of metals (although that is fine if they, like yours, seem to
understand your requirements fully) but the bullion dealers and metal
refiners who supply the jewellers. I don't know who they are in the US
or Australia, but here in the UK Johnson Matthey Metals would be the
first port of call. They can supply very pure Pt, Ir, Pd, Au, W, etc.

I don't fully understand your point about aluminium. If you are
wrapping it round tungsten wire, you are presumably then evaporating
it thermally rather than sputtering. So where does the argon come in?
Why don't you do this under ultra-high vacuum?

Best wishes
Chris

----- Original Message -----
} From: "Rosemary White" {rosemary.white-at-csiro.au}
To: "Tindall, Randy D." {TindallR-at-missouri.edu}
Cc: {microscopy-at-sparc5.microscopy.com}
Sent: Friday, June 14, 2002 12:48 AM


Hi Jerzy,

also look for potassium! Cells contain around 140 mM of K, which should translate to something in the range of 10 percent (biologists please correct me if I'm wrong) K atoms in the dry mass. Much more than Ca anyway.

Regards,
Heiko

------------------------------------------

Dr. Heiko Stegmann
AMD Saxony Manufacturing GmbH
Materials Analysis/TEM Group
Wilschdorfer Landstr. 101
01109 Dresden
Germany
Phone +49-351-2774167
Fax +49-351-2774199
e-mail heiko.stegmann-at-amd.com



Von: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Gesendet: Freitag, 14. Juni 2002 06:23
An: Microscopy-at-sparc5.microscopy.com
Betreff: Bacteria on semiconductor


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Experts,

as a materials scientist I don't have too much exposure to bio-elemental analysis. Could someone in Bio-fields help out. What elements should I expect to find in biological residue? I am evaluating suspected bacterial deposits (contamination) and finding O, C, and Ca. Would there be any other elements present after the residue was encapsulated in high temperature deposited silicon oxide?

Thank you in advance.

Warmest Regards from Texas.

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************











From daemon Fri Jun 14 04:41:18 2002



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 14 Jun 2002 10:32:39 +0000
Subject: Encapsulite safelights

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

I have been asked to specify safelights for a new darkroom and
TEM room. The old darkroom had a red Encapsulite fluorescent
strip, that was great for paper printing but we found that it was not
safe with EM Film. Is there an Encapsulite filter type you can
confidently recommend for use with Kodak SO163 EM Film, or
would you prefer something else? If so, what? The darkroom will
be 3x3metres, and ideally I want a diffuse, general room lighting
that will serve for both EM film loading and processing and bromide
paper printing.

Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Fri Jun 14 08:00:08 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 14 Jun 2002 08:55:13 -0400
Subject: Bacteria on semiconductor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jerzy;

I also deal in semiconductors, specifically failure analysis of same. On
occasion, I have to identify what appears to be an organic contaminant on
the surface or embedded into a structure such as a dielectric film, SiO2,
SiN etc. The tool that I find most useful for this is EDX [EDS] since these
contaminants generally detectable via SEM. Of course, the EDX system has
light element capability for O, C, etc.

More often than not, the elements you mentioned, C, O, Ca are present as
well as P, Cl, Mg, Fe. Also, many contaminants are simply skin flakes,
humans shed several million per day, which contain all of the above
elements. There is a reference to this in the Merck Index [composition of
skin/dermis/epidermis]. I can dig it out if you need it and it's a good
reference to substantiate or corroborate conclusions.

An additional nightmare in semiconductor contamination is cosmetics, e.g.
eye makeup, and that other powder stuff, I believe morticians also use it.
Since one cannot cover their eyes while looking through the eyepieces of a
microscope, little things like mascara break off of eyelashes and on to the
device. I think there is also a reference somewhere as to the elemental
composition of this stuff [makeup]. I'm sure most are proprietary
formulations like Helena Rubenstein's Stuff etc. but it's good to know
generally what's in these things.

The problem you may have with bacterial identification is the fact that
wafer processing uses high temperatures in deposition, annealing, plasma
treatment etc. At 750 C and higher, I doubt whether a bacteria will still
look like one if imaged in an SEM or optically. Imaging an organism is
probably the best identification of the "species", if that's the correct
term, if one can do so prior to all the high temp. processing.

I'm sure someone on the listserver, if not many, have great references on
SEM identification of bacteria.

Regards,

Peter Tomic
Team Leader
Analytical Dept.
Anadigics, Inc.





-----Original Message-----
} From: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Sent: Friday, June 14, 2002 12:23 AM
To: Microscopy-at-sparc5.microscopy.com


Experts,

as a materials scientist I don't have too much exposure to bio-elemental
analysis. Could someone in Bio-fields help out. What elements should I
expect to find in biological residue? I am evaluating suspected bacterial
deposits (contamination) and finding O, C, and Ca. Would there be any other
elements present after the residue was encapsulated in high temperature
deposited silicon oxide?

Thank you in advance.

Warmest Regards from Texas.

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White
Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From daemon Fri Jun 14 08:22:01 2002



From: Simon Dumbill :      simon.dumbill-at-aeat.co.uk (by way of
Date: Fri, 14 Jun 2002 08:12:00 -0500
Subject: TEM - SF6 disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Content-Type: text/html; charset=ISO-8859-1
Content-Description: HTML


We're in the process of planning the decommissioning of an EM430. Our
Safety and Environment people are not at all happy about the idea of
us just dumping the SF6 from the HT tank and, so far, we've not been
able to identify another disposal route. We've contacted refrigerant
disposal companies but SF6 is a new one to them and Air Products were
no use either. So if anyone out there has any experience of SF6
disposal I'd be very interested to hear from you.

Thanks,

Simon

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}

Dr Simon Dumbill
Team Leader, Microstructural Characterisation
AEA Technology Nuclear Science
B14, Windscale
Seascale
Cumbria CA20 1PF

Tel: +44 (0)19467 72235
Fax: +44 (0)19467 72606

Email: {mailto:Simon.Dumbill-at-aeat.co.uk} Simon.Dumbill-at-aeat.co.uk

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}


***********************************************************************
This transmission contains information which may be confidential and
which may also be privileged. It is intended for the named addressee
only. Unless you are the named addressee, or authorised to receive it
on behalf of the addressee you may not copy or use it, or disclose it
to anyone else. If you have received this transmission in error please
contact the sender. Thank you for your cooperation.
***********************************************************************

For more information about AEA Technology please visit our website at
http://www.aeat.co.uk

AEA Technology plc registered office 329 Harwell, Didcot, Oxfordshire OX11 0QJ.
Registered in England and Wales, number 3095862.


From daemon Fri Jun 14 09:58:49 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 14 Jun 2002 10:51:52 -0400
Subject: Re: Bacteria on semiconductor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers;

If anyone needs an EDX quantification of skin flakes, I have some on file.
However, there are so many variables in the quantification that I'm
uncertain if it would be useful. P & K are certainly among the elements of
skin.

Peter Tomic

-----Original Message-----
} From: "heiko.stegmann-at-amd.com"-at-sparc5.microscopy.com
[mailto:"heiko.stegmann-at-amd.com"-at-sparc5.microscopy.com]
Sent: Friday, June 14, 2002 3:48 AM
To: Microscopy-at-sparc5.microscopy.com


Hi Jerzy,

also look for potassium! Cells contain around 140 mM of K, which should
translate to something in the range of 10 percent (biologists please correct
me if I'm wrong) K atoms in the dry mass. Much more than Ca anyway.

Regards,
Heiko

------------------------------------------

Dr. Heiko Stegmann
AMD Saxony Manufacturing GmbH
Materials Analysis/TEM Group
Wilschdorfer Landstr. 101
01109 Dresden
Germany
Phone +49-351-2774167
Fax +49-351-2774199
e-mail heiko.stegmann-at-amd.com



Von: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Gesendet: Freitag, 14. Juni 2002 06:23
An: Microscopy-at-sparc5.microscopy.com
Betreff: Bacteria on semiconductor


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Experts,

as a materials scientist I don't have too much exposure to bio-elemental
analysis. Could someone in Bio-fields help out. What elements should I
expect to find in biological residue? I am evaluating suspected bacterial
deposits (contamination) and finding O, C, and Ca. Would there be any other
elements present after the residue was encapsulated in high temperature
deposited silicon oxide?

Thank you in advance.

Warmest Regards from Texas.

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White
Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************











From daemon Fri Jun 14 10:38:33 2002



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 14 Jun 2002 10:34:02 -0500
Subject: Re: SEM/ Cryo unit sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Geetings Randy, and to others on this "thread":

} We have an Emitech cryopreparation unit associated with our Hitachi S4700
} FESEM.

snip!

} Also, if anybody has any wisdom to share on avoiding getting ice on samples
} during cryo runs, I'd love to hear it. We often (usually) get ice during
} transfers and need to sublime off the moisture before coating, otherwise the
} coating forms quasi-permanent ice "casts" that render the sample unusable.
} We've replaced/checked all the o-rings we can reach, so any special hints are
} very welcome.

On my Hitachi S3500N SEM, I have the Emitech K1150 cryo-prep unit, the
"little sibling" to the K1250, which I'll bet is the one you have? Mine
mounts onto the SEM sample chamber and consists of has air lock transfer
into a sputter coating chamber, then on into the SEM. The SEM vacuum system
is used for this unit, and I have to bleed the argon through the entire SEM,
as well as the sputter coater, but it works fine that way. The LN
cryo-freezing unit is a small seperate bench-top device, so I do need to
make a transfer from that, in room air, into the SEM mounted sputter coater
& air lock transfer unit. My system comes with a metal frostproofing shroud
which is picked up at the bottom of the LN freezing unit immediately after
the freeze and it tightly surrounds the sample and its stub in a nitrogen
gas atmosphere and prevents room air and humidity from getting to sample, so
I almost never see frost at the low to moderate mags we typically use. I
would think your system would also have such protection, so I wonder if
maybe your samples have surface moisture that looks like frost after
freezing?

In the event of frost contamination, here is a cheap trick that I use to
sublimate it off the surface once its in the SEM: Just hook up a bottle of
dry nitrogen gas to the air input of your SEM, via a regulator set to about
3-8 psi. I you already use N gas to vent your SEM, you're all set! Vent to
atmosphere and then immediately pump back down. All the while the cryostage
is kept at usual maximum cold temperature, about -120 C in my case, but the
gas imparts just enough thermal energy to the frost to sublimate it off. You
may not even have to vent all the way to room pressure before pumping back
down - experiment and see what works.

The big advantage is this only takes me 4 minutes for complete cycle.
Disadvantage is you must turn the beam off so you can't watch, but not a
problem. I don't like to defrost by heating up the stage and the entire mass
of the sample just to get some heat to the surface frost, as it takes so
long to do that plus cool down again, and you run the risk of overheating
and drying out the surface of the sample. Also with the K1150, because the
sputter coater is mounted to the SEM, if I should need to resputter after a
defrost, I just pull it back into the sputter chamber and there is no
transfer in air involved. Again, its rare that I have to do this now, on my
current system. Before, on my old Philips 500 SEM, I had to do air transfers
with NO frost shroud, so I did this N gas defrost more often.

Good luck!

Gib

} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
-- --------------------------
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Fri Jun 14 10:49:50 2002



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 14 Jun 2002 10:46:08 -0500
Subject: Re: SEM/ Cryo unit sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I forgot to add this reference for my nitrogen gas defrost to my recent
posting on this topic. The pictures show an example of the results obtained:

Low-temperature low-voltage scanning electron microscopy of uncoated frozen
biological materials: A simple alternative. 1996. Microscopy &
Microanalysis. pp 918-19. (Minneapolis MSA meeting).

Reprints are still available!! (plug plug).

Gib

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Fri Jun 14 12:04:57 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 14 Jun 2002 09:56:27 -0700 (PDT)
Subject: Re: TEM - SF6 disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think recycling SF6 through a company that does such work is the best
option.

Here is an article about SF6 I found useful:

Many people have heard of the environmental impact of greenhouse gas
emissions, such as chlorofluorocarbons. As a result, we have seen design
modifications in product packaging, such as changing aerosol propellants
to pumps and other environmentally safe dispensers. The IEEE has also been
concerned with these greenhouse gases, and in particular with sulfur
hexafluoride (SF6), which is commonly used as a dielectric and insulator
in circuit breakers, gas-insulated substations (GIS), and related
equipment for electrical transmission and distribution systems. According
to the Environmental Protection Agency (EPA), SF6 is a desirable gas
because of its stability when used in these electrical systems, but it has
undesirable properties when released into the environment. It has a long
life of about 3200 years, according to the estimates of the
Intergovernment-al Panel on Climate Change (IPCC). It is one of the worst
offenders of the greenhouse gases, having the highest global warming
potential.

Currently, there are no regulations regarding the disposal of SF6.
However, IEEE standards are encouraging manufacturers to safeguard the
release of SF6 into the air. IEEE Std C37.122.1-1993, a guide to GIS,
recommends against the unnecessary release of SF6 into the atmosphere
because of environmental concerns. Draft standard IEEE P1403, which
compares air-insulated substations and GIS, mentions that recent advances
in GIS construction include sophisticated equipment needed to reprocess
SF6. This recommendation means that less SF6 is released into the
atmosphere, helping to alleviate environmental concerns over the release
of greenhouse gases.

The EPA also has been involved with the subject of SF6. In August 1995, it
hosted a conference entitled Electrical Transmission and Distribution
Systems--Sulfur Hexafluoride and the Atmospheric Effects of Greenhouse Gas
Emissions. This international meeting included IEEE members as attendees.
The session urged voluntary compliance among users of SF6 so that further
official regulation is not needed in this area. This session also helped
to raise the visibility of SF6 disposal as a growing area of environmental
concern. Expect to see more discussion of SF6 disposal in the future.

For the final proceedings of the EPA greenhouse gas emissions conference,
contact the Atmospheric Pollution Prevention Division; US EPA 6202J;
Washington, DC 20460.


\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Fri, 14 Jun 2002, Simon Dumbill wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Content-Type: text/html; charset=ISO-8859-1
} Content-Description: HTML
}
}
} We're in the process of planning the decommissioning of an EM430. Our
} Safety and Environment people are not at all happy about the idea of
} us just dumping the SF6 from the HT tank and, so far, we've not been
} able to identify another disposal route. We've contacted refrigerant
} disposal companies but SF6 is a new one to them and Air Products were
} no use either. So if anyone out there has any experience of SF6
} disposal I'd be very interested to hear from you.
}
} Thanks,
}
} Simon
}
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
}
} Dr Simon Dumbill
} Team Leader, Microstructural Characterisation
} AEA Technology Nuclear Science
} B14, Windscale
} Seascale
} Cumbria CA20 1PF
}
} Tel: +44 (0)19467 72235
} Fax: +44 (0)19467 72606
}
} Email: {mailto:Simon.Dumbill-at-aeat.co.uk} Simon.Dumbill-at-aeat.co.uk
}
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
}
}
} ***********************************************************************
} This transmission contains information which may be confidential and
} which may also be privileged. It is intended for the named addressee
} only. Unless you are the named addressee, or authorised to receive it
} on behalf of the addressee you may not copy or use it, or disclose it
} to anyone else. If you have received this transmission in error please
} contact the sender. Thank you for your cooperation.
} ***********************************************************************
}
} For more information about AEA Technology please visit our website at
} http://www.aeat.co.uk
}
} AEA Technology plc registered office 329 Harwell, Didcot, Oxfordshire OX11 0QJ.
} Registered in England and Wales, number 3095862.
}



From daemon Fri Jun 14 12:38:38 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 14 Jun 2002 11:38:33 -0600
Subject: Bacteria on semiconductor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jerzy,

There are two other sources (probably more) for the contamination besides
bacteria or the materials that Peter mentioned:

1) Algae. When I worked on semiconductors, we had a clean room where some
parts of the DI Water lines used transparent hoses. We found algae growing
in the DI water.

2) The elements you mention (O, C, Ca) can also come from Calcium carbonate.
I had at one time some dirt on my wafers that included the same materials.
The dirt consisted of roundish contamination areas, some with a ring-like
structure. It turned out that we had slightly hard water and we did not dry
our materials carefully enough to avoid small droplets remaining on the
material. Further heat treatment then evaporated the water and left the
residue.

mike


} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Sent: Thursday, June 13, 2002 10:23 PM
To: Microscopy-at-sparc5.microscopy.com


Experts,

as a materials scientist I don't have too much exposure to bio-elemental
analysis. Could someone in Bio-fields help out. What elements should I
expect to find in biological residue? I am evaluating suspected bacterial
deposits (contamination) and finding O, C, and Ca. Would there be any other
elements present after the residue was encapsulated in high temperature
deposited silicon oxide?

Thank you in advance.

Warmest Regards from Texas.

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White
Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From daemon Fri Jun 14 13:25:53 2002



From: sghoshro-at-NMSU.Edu
Date: Fri, 14 Jun 2002 12:19:36 -0600 (MDT)
Subject: Re: Encapsulite safelights

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

We use Kodak light amber safelight for both EM film and paper and we use
Kodak polycontrast printing paper and 4489 EM films. I am quite sure the
light amber is safe for SO-163 and bromide paper.

Best wishes,

Soumitra

*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml

On Fri, 14 Jun 2002, Chris Jeffree wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All
}
} I have been asked to specify safelights for a new darkroom and
} TEM room. The old darkroom had a red Encapsulite fluorescent
} strip, that was great for paper printing but we found that it was not
} safe with EM Film. Is there an Encapsulite filter type you can
} confidently recommend for use with Kodak SO163 EM Film, or
} would you prefer something else? If so, what? The darkroom will
} be 3x3metres, and ideally I want a diffuse, general room lighting
} that will serve for both EM film loading and processing and bromide
} paper printing.
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================
}
}



From daemon Fri Jun 14 14:05:54 2002



From: jerzy.gazda-at-amd.com
Date: Fri, 14 Jun 2002 13:59:16 -0500
Subject: Bacteria on semiconductor - thank you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all that have replayed to my question from last night.

For trade reasons I cannot discuss all the details of the defect I am investigating, but it is sufficient to say, we traced its formation to one of water based wet cleans. I was provided samples that had defects encapsulated into layers of Si-oxide and went through high temperature processes. Therefore the microorganism was burned up and resulting residue does not have structure (amorphous blob of darker contrast in oxide). Based on your replays, I will follow up with analysis on blanket Si wafers processed in the same sink and attempt to image the micro-organism with SEM. Hopefully the structure will be preserved. Elements listed in your replays are: H, C, O, K, Ca, Na, and if bacteria is metal reducing S, Fe, etc.

Again, thank you all for your help.

Even warmer Regards from Texas (104F).

Jerzy

******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From daemon Fri Jun 14 15:38:03 2002



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Fri, 14 Jun 2002 16:10:22 -0400
Subject: SEM - Use of Cyro-transfer/Sputter system. Fee for use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Afternoon,
I would like to hear from anyone who can help me locate a lab having a SEM
equipped with a cryo-transfer/sputter system. I am interested in examining
small (5 to 10 micron) polymer particles that can be fractured at low
temperature then coated and subsequently examined, preferably at low ( {5kV)
accelerating voltage. Hopefully such a lab/facility exists in Southern
Ontario, somewhere between Toronto and Hamilton would be great. Thanks.

Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com



From daemon Fri Jun 14 20:00:26 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 14 Jun 2002 17:51:58 -0700
Subject: RE: TEM - SF6 disposal

Contents Retrieved from Microscopy Listserver Archives
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Simon;
Our service engineer piped it into a plastic garbage bag, which he
carried outside and dumped. This is relatively easy because SF6 is
considerably denser than air, and safer than dumping it in the room, since
the HVAC would have difficulty eliminating it. Our new labs have the SF6
bleed plumbed to scrubbed exhaust.

John Mardinly
Intel




Content-Type: text/html; charset=ISO-8859-1
Content-Description: HTML


We're in the process of planning the decommissioning of an EM430. Our
Safety and Environment people are not at all happy about the idea of
us just dumping the SF6 from the HT tank and, so far, we've not been
able to identify another disposal route. We've contacted refrigerant
disposal companies but SF6 is a new one to them and Air Products were
no use either. So if anyone out there has any experience of SF6
disposal I'd be very interested to hear from you.

Thanks,

Simon

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}

Dr Simon Dumbill
Team Leader, Microstructural Characterisation
AEA Technology Nuclear Science
B14, Windscale
Seascale
Cumbria CA20 1PF

Tel: +44 (0)19467 72235
Fax: +44 (0)19467 72606

Email: {mailto:Simon.Dumbill-at-aeat.co.uk} Simon.Dumbill-at-aeat.co.uk

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}


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From daemon Sat Jun 15 01:53:52 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 14 Jun 2002 23:39:31 -0400
Subject: Re: TEM - SF6 disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 6/14/02 9:12 AM, Simon Dumbill (by way of MicroscopyListserver) at
simon.dumbill-at-aeat.co.uk wrote:

} We're in the process of planning the decommissioning of an EM430. Our
} Safety and Environment people are not at all happy about the idea of
} us just dumping the SF6 from the HT tank and, so far, we've not been
} able to identify another disposal route. We've contacted refrigerant
} disposal companies but SF6 is a new one to them and Air Products were
} no use either. So if anyone out there has any experience of SF6
} disposal I'd be very interested to hear from you.
}
} Thanks,
}
Dear Simon,
One thing to try is to freeze the SF6 into an empty gas bottle by
connecting the tank, regulator, etc. to a fitting on the HT tank, then
putting the bottom of the gas bottle in a bucket of dry ice (assuming that
the S&E people are not alarmed at the resulting CO2). At room temp, the SF6
will be mostly in the liquid state with some gas at 330 psi--its vapor
pressure. The SF6 is pretty valuable, so you might easily find someone to
take it off your hands. Good luck.
Yours,
Bill Tivol



From daemon Mon Jun 17 03:38:51 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Mon, 17 Jun 2002 18:21:02 +1000
Subject: Re: SEM/ Cryo unit sputter coater

Contents Retrieved from Microscopy Listserver Archives
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Chris,

Yes, we only use this freezing method for fairly rigid smallish plants and
insects or their parts. We call it slow-freezing because it's slower than
plunging in liquid ethane or propane (lab) or nitrogen (field) or snap
freezing between pliers cooled in LN2. But it takes less than a minute,
usually only a few seconds, for the tissue to freeze in the prep chamber,
so they don't lose too much surface water, not enough to damage the surface
structure, at least. Once it's frozen, it wouldn't lose more than a
plunge-frozen specimen, I shouldn't think. We don't deal with cultured
animal cells at all, for SEM at least, and I'd be wary of recommending this
method for soft and/or wet tissues (might be worth trying once, though).
And when we need to do analysis, the plunge frozen tissue is first planed
to give a fresh "clean" flat surface. Then we do need to get rid of frost
and also etch very lightly.

Re metals, Alfa Aeser is now controlled by Johnson Matthey, I think, and is
certainly our first port of call for other than gold, which we have been
able to get at highest purity from the local jeweller.

Sorry, brain disengaged re. Al coating - of course, this is evaporative
coating..... so we have to clean the chamber windows every now and then,
before they turn into mirrors.
cheers,
Rosemary



Rosemary
While I concede the slow-freeze method works on some specimens - it
can as you indicate be
particularly successful with leaves or small insects that are covered
with a cuticle -
such recidivism should only be practised by consenting adults fully
aware of the implications, which are
a) wet stuff (possibly including organic solvents) has to be
introduced to the high vacuum of the prep chamber thus contaminating
cold stage and anticontaminator of the prep chamber
b) uncontrolled evaporation from the surface of the un-fixed specimen
c) thus specimens without the benefit of a protective covering (such
as cultured cells) will show variable amounts of drying artefacts.
I suppose the frank reality is that all etching / drying procedures
result in artefacts, but there is a comforting illusion of control if
you can get the same artefacts with reasonable reproducibility!

On the topic of gold for targets, I was not recommending jewellers as
a source of metals (although that is fine if they, like yours, seem to
understand your requirements fully) but the bullion dealers and metal
refiners who supply the jewellers. I don't know who they are in the US
or Australia, but here in the UK Johnson Matthey Metals would be the
first port of call. They can supply very pure Pt, Ir, Pd, Au, W, etc.

I don't fully understand your point about aluminium. If you are
wrapping it round tungsten wire, you are presumably then evaporating
it thermally rather than sputtering. So where does the argon come in?
Why don't you do this under ultra-high vacuum?

Best wishes
Chris





From daemon Mon Jun 17 06:45:53 2002



From: George Laing :      scisales-at-ngscorp.com
Date: Mon, 17 Jun 2002 07:37:51 -0400
Subject: RE: Encapsulite safelights

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


According to Kodak, the correct safelight for SO-163 film is either
the GBX-2 or a #1(red). For 4489, the correct safelight filters are
either the OC(amber) or OA(greenish yellow).
For more safelight information see
http://www.kodak.com/US/en/health/scientific/products/electronmicrography/da
rkroom.shtml

Encapsulite has filters for almost every use. They also have a
dual lamp fixture for those needing two filters.
See http://www.encapsulite.com/doublelamp.htm If you contact them, they
can give you their equivalent to the GBX-2.


George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com
}
} Is there an Encapsulite filter type you can
} confidently recommend for use with Kodak SO163 EM Film, or
} would you prefer something else? If so, what? The darkroom will
} be 3x3metres, and ideally I want a diffuse, general room lighting
} that will serve for both EM film loading and processing and bromide
} paper printing.
}




From daemon Mon Jun 17 08:12:52 2002



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Mon, 17 Jun 2002 09:04:59 -0400
Subject: Looking for Jan Schwarz

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have her email address? She's located in Vermont.

Thanks!

Lesley Bechtold



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Mon Jun 17 12:27:26 2002



From: Mae :      pqqljxitg-at-cci.be
Date: Tue, 04 Jun 2002 08:47:29 +0900
Subject: Learn how to Outperform the Dow Jones industrial average

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Mon Jun 17 13:48:44 2002



From: hard-at-acsu.buffalo.edu
Date: Mon, 17 Jun 2002 14:38:17 -0500
Subject: Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Course Announcement

Title: Optical Microscopy and Imaging in the Biomedical Sciences

When: October 9 - October 18, 2002

Where: Marine Biology Laboratory, Woods Hole, MA, USA

Tuition: $2200 (Includes room and board, text, handouts, supplies)

Application Deadline: July 25, 2002

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Internet: admissions-at-mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

Course Director: Colin S. Izzard, State University of New York -at- Albany
Phone: [518] 442 - 4367
EMail: csizzard-at-csc.albany.edu

Course Description:

For Whom:
Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and video-imaging will benefit greatly from this course as well.
There are no specific prerequisites, but an understanding of the basic
principles of optics is desirable. Limited to 24 students.

The eight day course consists of lectures, laboratory demonstrations,
exercises and discussions that will enable the participant to obtain and
interpret microscope images of high quality, to perform quantitative optical

measurements, and to produce photographic and video records for
documentation
and analysis.

Topics to be covered include:
principles of microscope design and image formation
bright field, dark field, phase contrast, polarized light,
differential
interference contrast, interference reflection, and fluorescence
microscopy
confocal scanning microscopy, multiphoton excitation fluorescence
microscopy and image deconvolution
digital image restoration and 3-D reconstruction
video imaging, recording, enhancement, and intensification
analog and digital image processing and analysis
fluorescent probes and ratiometric-imaging
laser tweezers and laser scissors

Applications to live cells will be emphasized; other specimens will be
covered as well.

Students will have direct hands-on experience with state-of-the-art
microscopes, video cameras, recorders and image processing equipment
provided
by major optical and electronics companies. Instruction will be provided by
experienced staff from universities and industry.

STUDENTS ARE ENCOURAGED TO BRING THEIR OWN BIOLOGICAL (PRIMARY
CULTURES, CELL LINES, PREPARED SLIDES, ETC.) AND MATERIAL SPECIMENS AND TO
USE THEM FOR COURSE EXERCISES, WHERE APPROPRIATE. Cell culture facilities
are available. Students are highly encouraged to discuss individual research
problems with the academic and commercial faculty.

For faculty list and additional information, see: http://www.mbl.edu




From daemon Mon Jun 17 15:39:13 2002



From: mohammed y abdulrawoof / f40z006 75760 :      mdyousuf-at-KSU.EDU.SA
Date: Mon, 17 Jun 2002 23:23:45 -0300 (GMT)
Subject: Thanks and appreciation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
This list server is of great help, when cornered into bottle- necks.
Thanks to every one who replied to my Osmium Tetroxide question. Thanks to
all the expert advise, which helped me in using the many ampules which
were just about to be discarded from the stores. My deep regards to
Nestor who keeps the show running.

Mohammed Yousuf Abdul-Rawoof
Dept. of Zoology
College of Science
King Saud University
POB 2455, Riyadh 11451
Saudi Arabia.

mdyousuf-at-ksu.edu.sa
mdyousuf99-at-hotmail.com



From daemon Tue Jun 18 08:29:26 2002



From: PHYSIOL4-at-AKAD.SUN.AC.ZA
Date: Tue, 18 Jun 2002 15:11:09 +0200
Subject: Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

Thanks for all your ideas and input, I am currently at the end stages
of attempting to fix sugarcane in a 4% paraformaldehyde, 0.5%
glutaraldehyde mixture (I think Jan Coetzee suggested I try that),
and will let you know how that went. Thanks also to Rosemary White
for that reference about in situs on non-embedded tissues, will
definitely check that out.

Cheers!
Gabrielle


Gabrielle Turner
Institute for Plant Biotechnology
University of Stellenbosch
7600

Cell: 083 324 7453


From daemon Tue Jun 18 16:56:34 2002



From: Chun-Ming Li :      lichun-at-uiuc.edu
Date: Tue, 18 Jun 2002 16:41:41 -0500
Subject: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear friends:
I have a problem in preparing a TEM sample (plan view). The
as-received sample is as follow:
On a soda-lime glass (thickness 2-4 mm) substrate, deposit a
molybdenum layer (thickness 0.1-1.5 micron), and then deposit a layer of
CuInSe2 (thickness 0.7-2.5 micron).
After dissolved the glass with HF (hydrofluoric) acid (cover the layers
with lacquer before etching, otherwise the multilayers will also be
dissolved), I immersed the multilayers into acetone. But the layers are
too brittle that they broke into tiny chips. Even sometimes I can get a
big chip, it is still very difficult to fix it on a grid, since it is
too brittle.
I would appreciate it very much if you can give me any suggestions
to solve this problem, i.e., how to get a plan-view TEM sample from the
as-received sample. The major problem would be how to get rid of the
glass and fix the multilayers to grid before ion milling.
Thank you in advance!

Chun-Ming Li, Ph.D.
Dept. of Mater. Sci. and Engi.
University of Illinois at Urbana-Champaign




From daemon Tue Jun 18 23:00:25 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 18 Jun 2002 23:35:50 -0400
Subject: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You do not have to dissolve the glass. You can get the layers off by suspending the sample in the HF vapors for a while with plastic tongs and then gently floating the samples off in water. A small amount of HF in a plastic beaker will work fine. You should have small chips to work with so that the HF vapors do not have to penetrate far. You can then float the films off in water much like you do with films on salt crystals. For better support, use a high mesh grid, 400 of more. You can also get a little better support with holey carbon support films on the grids. Once the samples are on the grids, you will have to dissolve the lacquer. For picking the films up from the water, you should try using a wire loop as it will be more gentle on your fragile samples than trying to pick them up on the grids directly. Electron Microscopy Science sells the "Perfect Loop" which might work well for your application.

One issue you have though is that at 1.5 um, the samples will not be electron transparent, but I assume that you have thought of that.

Be very careful with HF! Do not let it come in contact with your skin and do not breath the vapors!



-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Chun-Ming Li [mailto:lichun-at-uiuc.edu]
Sent: Tuesday, June 18, 2002 5:42 PM
To: Microscopy-at-sparc5.microscopy.com


Dear friends:
I have a problem in preparing a TEM sample (plan view). The
as-received sample is as follow:
On a soda-lime glass (thickness 2-4 mm) substrate, deposit a
molybdenum layer (thickness 0.1-1.5 micron), and then deposit a layer of
CuInSe2 (thickness 0.7-2.5 micron).
After dissolved the glass with HF (hydrofluoric) acid (cover the layers
with lacquer before etching, otherwise the multilayers will also be
dissolved), I immersed the multilayers into acetone. But the layers are
too brittle that they broke into tiny chips. Even sometimes I can get a
big chip, it is still very difficult to fix it on a grid, since it is
too brittle.
I would appreciate it very much if you can give me any suggestions
to solve this problem, i.e., how to get a plan-view TEM sample from the
as-received sample. The major problem would be how to get rid of the
glass and fix the multilayers to grid before ion milling.
Thank you in advance!

Chun-Ming Li, Ph.D.
Dept. of Mater. Sci. and Engi.
University of Illinois at Urbana-Champaign




From daemon Wed Jun 19 02:47:37 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Wed, 19 Jun 2002 08:38:18 +0100
Subject: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chun-Ming,
One way to pick up small thin fragments is to use a mesh grid with a
thin coating of epoxy. I use Devcon 5-minute epoxy. Choose a mesh grid
with holes smaller than your typical fragment size. Place it on filter
paper and cover it with a blob of glue. Then take another piece of filter
paper and press down on top to remove some glue. Separate the papers, move
the grid to a clean region of paper, and repeat 2 or 3 times. This will
give you a 'tacky' mesh grid with no epoxy covering the holes. You can then
use this to pick up fragments of film.
When looking at thin films on glass I have usually tried to make the
amount of glass to be dissolved as small as possible by grinding from the
back to a thickness of a grade zero cover slip (~120 um). You can then
dissolve the glass in a small amount of HF, which can be diluted massively
when the glass is gone, and filtered through coarse filter paper to collect
the fragments. When the paper is dry you can use a tacky grid to pick up
the fragments. In your case, I guess you would take the lacquer sheet (with
film attached) into a solvent and filter the solvent when the lacquer is
dissolved. Again massive dilution may help, in this case to reduce the
amount of lacquer which dries on the fragments.

Good luck,


Richard Beanland

__________________________
Richard Beanland
Analytical Services,
Bookham Technology plc.,
Caswell,
Towcester,
Northants NN12 8EQ
UK
e-mail richard.beanland-at-bookham.com



-----Original Message-----
} From: Chun-Ming Li [mailto:lichun-at-uiuc.edu]
Sent: 18 June 2002 22:42
To: Microscopy-at-sparc5.microscopy.com


Dear friends:
I have a problem in preparing a TEM sample (plan view). The
as-received sample is as follow:
On a soda-lime glass (thickness 2-4 mm) substrate, deposit a
molybdenum layer (thickness 0.1-1.5 micron), and then deposit a layer of
CuInSe2 (thickness 0.7-2.5 micron).
After dissolved the glass with HF (hydrofluoric) acid (cover the layers
with lacquer before etching, otherwise the multilayers will also be
dissolved), I immersed the multilayers into acetone. But the layers are
too brittle that they broke into tiny chips. Even sometimes I can get a
big chip, it is still very difficult to fix it on a grid, since it is
too brittle.
I would appreciate it very much if you can give me any suggestions
to solve this problem, i.e., how to get a plan-view TEM sample from the
as-received sample. The major problem would be how to get rid of the
glass and fix the multilayers to grid before ion milling.
Thank you in advance!

Chun-Ming Li, Ph.D.
Dept. of Mater. Sci. and Engi.
University of Illinois at Urbana-Champaign




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From daemon Wed Jun 19 06:18:31 2002



From: Sousan Abolhassani :      sousan.abolhassani-at-psi.ch
Date: Wed, 19 Jun 2002 13:07:37 +0200
Subject: Re: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chun-Ming,

As your multi layer is thick, in any case you should thin it
afterwards.
Instead of going through the problem of dissolving and collecting
such a layer that you still need to thin by other techniques
there are at least three possibilities (that do not need suffisticated
facilities):

a. If you want to see the top view of your multi-layer, then
it means that you should thin your sample from the molybdenum
side until it is electron transparent. For this, the sample
should be grinded from the glass side to a thickness of the order
of 20-50 microns, then mounted on a ring sample holder used
for ion milling. (If you can grind down to 30 micrometers it needs a
shorter time for ion-milling, but if you cann't it is not important).
Then you ion-mill your sample from the glass side, until you see a hole
and you know that the thinnest part is in the CuInSe2 region.

Note that in this case you will not see the molybdenum, because your
CuInSe2 is 0.7-2.5 micrometers as you say.
If you wish to observe the molybdenum as well, you could take another
sample and proceed as above, but stop milling when the sample is
near the Mo (you could see from colour changes) and then ion mill
from the other side.

b. The second possibility is to do a tripod polishing and this can
be done with different orientations. All layers will be observable
and from my experience this is the most convenient method, that
does not need to complicated facilities.

c. The last method, that I use for quick determinations of the
material before the above elaborate methods, is to scratch the
surface to be observed by a diamond pointer and then put the
sctratched debris in a solvent that does not attack the film
(you should use a very small recipient not to loose your material)
and collect the debris on a grid with a thin support film.
This method is very rapid and can quickly bring some information
before you proceed with more elaborate techniques.


Hope this helps,

Sousan



E-mail: sousan.abolhassani-at-psi.ch
http://www.psi.ch


Chun-Ming Li wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear friends:
} I have a problem in preparing a TEM sample (plan view). The
} as-received sample is as follow:
} On a soda-lime glass (thickness 2-4 mm) substrate, deposit a
} molybdenum layer (thickness 0.1-1.5 micron), and then deposit a layer of
} CuInSe2 (thickness 0.7-2.5 micron).
} After dissolved the glass with HF (hydrofluoric) acid (cover the layers
} with lacquer before etching, otherwise the multilayers will also be
} dissolved), I immersed the multilayers into acetone. But the layers are
} too brittle that they broke into tiny chips. Even sometimes I can get a
} big chip, it is still very difficult to fix it on a grid, since it is
} too brittle.
} I would appreciate it very much if you can give me any suggestions
} to solve this problem, i.e., how to get a plan-view TEM sample from the
} as-received sample. The major problem would be how to get rid of the
} glass and fix the multilayers to grid before ion milling.
} Thank you in advance!
}
} Chun-Ming Li, Ph.D.
} Dept. of Mater. Sci. and Engi.
} University of Illinois at Urbana-Champaign


From daemon Wed Jun 19 07:26:37 2002



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 19 Jun 2002 07:23:38 -0500 (CDT)
Subject: PEELS on a PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a PC compatible interface for a Gatan
PEELS that they would be willing to donate (or sell) to
a worthy cause? I would also be interested in a Mac version,
although I would prefer a PC.

-----------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2225 N Campus Drive
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
-----------------------------------------------




From daemon Wed Jun 19 07:57:44 2002



From: Gabriel A. Rosa :      micros-at-bg.fcen.uba.ar
Date: Wed, 19 Jun 2002 09:51:01 -0300
Subject: I need help (spectrofluorometers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,
My name is Gabriel, and in my University (of Buenos Aires) are two
spectrofluorometers (one aminco SPF500 and one Perkin Elmer 650-40) both
without electric source for the lamps.
Anything have an electric source for this equipment for donation?
Or anything have the electrical chart for construct this??

T.I.A.

Gabriel Adriano Rosa
Centro de Imagenes y Microscopia
Departamento de Ciencias Biologicas
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires
Ciudad Universitaria, 4 piso, Pab. II,
CP 1428, Buenos Aires, ARGENTINA
TE -(54-11)-4576-3349
FAX (54-11)-4576-3384
e-mail cimic-at-bg.fcen.uba.ar






From daemon Wed Jun 19 09:49:40 2002



From: John Mansfield :      jfmjfm-at-umich.edu
Date: Wed, 19 Jun 2002 10:35:20 -0400
Subject: Gatan DuoMill parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there,

We are still running a 14+ year old Gatan DuoMill and are having a few
problems with one of the Whisperloks leaking a little. There is a
Swagelock seal on the bottom of the whole assembly where the braid attaches
for the liquid nitrogen dewar for cold milling. This seal is so tightly
bound to the aluminum rod that runs through the center of the whole thing
that we cannot take the Swagelock stuff off to try and replace the ferrule
or the aluminum rod to try and improve the seal.

My question is, does anyone have a DuoMill that they have retired that we
could buy/scrounge parts from to try and fix this?

I can go to Gatan and buy a new part but I think that they may have to have
them made since they no longer make the DuoMill and it seems they don't have
this part in stock. Having one made will probably make this rather
expensive and I am trying to do this on the cheap. Can anyone be of
assistance?

ADVthanksANCE.

Jfm

--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"




From daemon Wed Jun 19 13:28:46 2002



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Wed, 19 Jun 2002 14:21:51 -0400
Subject: Jet polishing recipe for stainless steel 316LN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I need to make TEM samples from the bulk stainless steel.
I would appreciate very much if anyone could give me the recipe to make TEM
samples of stainless steel 316LN using jet polishing.

Thanks

Regards
Yan xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Wed Jun 19 15:33:25 2002



From: Krzysztof Herman :      kherman-at-labsoft.com.pl
Date: Wed, 19 Jun 2002 22:26:37 +0200
Subject: Philips EM420 IGP Power Supply

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List

Does somebody have the "IGP power supply" (or maybe some of it's components) to sell cheap
or even donate to Czech microscopists ??

(I don't count on many offers ;-)) ... but to ask is allways worthwise..

regards

Krzysztof Herman
Labsoft, ul.Ba¿ancia 45A
02-892 Warszawa, Poland
tel/fx: (+48 22)6449753, 6449750
mobile: (+48 601)307456





From daemon Wed Jun 19 16:33:25 2002



From: Dmrelion-at-aol.com
Date: Wed, 19 Jun 2002 17:26:19 EDT
Subject: immersion oil removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear list members,

One of my associates has a large number of thin sections of limestone that he
wants to look at with cathodoluminescence (CL). The problem is that the thin
sections have been coated already with immersion oil for other microscopy
observations and this may create problems with the vacuum and electron beam
in the CL apparatus. Does anyone have suggestions on the best/easiest way to
remove the immersion oil? The oil is identified as "Immersion oil for
Microscopy, Cargille Type A, Formula Code 1248" and is said to be made up of
synthetic hydrocarbons and natural petroleum derivatives.

He will appreciate any and all suggestions.

Thank you.

Don Marshall

RELION Industries
PO Box 12
Bedford, MA 01730

781-275-4695 (Phone)
781-271-0252 (FAX)

dmrelion-at-aol.com

cathodoluminescence, mass spectroscopy, electron beam technology

http://www.excitingelectrons.com


From daemon Wed Jun 19 16:38:23 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 19 Jun 2002 17:31:45 -0400
Subject: FW: Darkroom safelights

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In spite of the fact that I think darkrooms are going to disappear either
because Kodak and friends go the way of Polaroid or because the digital
detectors get so good that they equal (though I can't see how!!!) the
resolution of film, I am following Chris' suggestion to put my response to
his safelight question on the list.

I have done this search before, so it wasn't just for his benefit. I
especially liked my find of the Butzi review which included some info about
LCD safelights which I fortuitously found again in the next to last
reference I gave.

Well, here it is, and I hope it's useful for others. I already have my
Thomas and I have the spare bulb. Our 20' x 15' of darkroom is completely
ready for all the students who will be using our new FEI Tachni 12T with its
Gatan 1000 side-mounted CCD camera. My analysis of the cost comparison for
a class of twenty between the CCD and Kodak 4489 has the Space Committee
already salivating about unused space. Unfortunately, the Polymer Chemists
are insisting on 4489 as are the Physicists. So, I'm ordering a 5' round
table so the rest of us can play poker while the few hard-timers are taking
pictures on the scope. they will be able to process during our breaks. I
am also generating a complete description of the original method of
agitation that was replaced by nitrogen burst technology which is no longer
cost effective at all.

Regards to all,

Fred



} ----------
} From: Monson, Frederick C.
} Sent: Wednesday, June 19, 2002 12:32 PM
} To: 'c.jeffree-at-ed.ac.uk'
} Subject: RE: Encapsulite safelights
}
} Morning Chris,
} I have been using a Thomas Super Duplex (~$200) for quite a long
} time, and I have noticed no problems with it an Kodak 4489. I have not
} tried it with SO163 or brands other than Kodak. I give you a site on
} which it is critiqued: http://www.butzi.net/reviews/darkroom.htm.
}
} Kodak Darkroom hints are very helpful at design time.
} http://www.kodak.com/cluster/global/en/consumer/products/techInfo/k4/k4Pla
} cement.shtml
}
} Safelight Recommendations - note the Table link
} http://www.kodak.com/global/en/consumer/products/techInfo/k4/k4Recomnd.sht
} ml#93831
}
} And more:
} http://www.silverprint.co.uk/dark19.html
}
} Ted Pella's ~$200 Duplex for $300
} http://www.tedpella.com/photo_html/photo10.htm
}
} LED Safelights:
} http://www.barbierielectronic.com/products/Safelights_en.htm
}
} Thomas at B&H
} http://198.77.206.55/links/534.html Choose {Thomas DUB ....} to get to
} good info on Thomas systems. Really hard to get good info on this stuff.
}
} Hope this helps,
}
} Regards,
}
} Fred
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} West Chester University
} South Church Street and Rosedale
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
}
}
}
}
}
}
} ----------
} From: Chris Jeffree
} Reply To: c.jeffree-at-ed.ac.uk
} Sent: Friday, June 14, 2002 6:32 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Encapsulite safelights
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All
}
} I have been asked to specify safelights for a new darkroom and
} TEM room. The old darkroom had a red Encapsulite fluorescent
} strip, that was great for paper printing but we found that it was not
} safe with EM Film. Is there an Encapsulite filter type you can
} confidently recommend for use with Kodak SO163 EM Film, or
} would you prefer something else? If so, what? The darkroom will
} be 3x3metres, and ideally I want a diffuse, general room lighting
} that will serve for both EM film loading and processing and bromide
} paper printing.
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================
}
}
}


From daemon Wed Jun 19 19:13:33 2002



From: rszilard-at-sickkids.ca (rachel szilard)
Date: Wed, 19 Jun 2002 20:05:42 -0400
Subject: LM - uncooperative antibodies & immunofluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am fairly new to staining tissues (mouse) and I am having trouble
detecting my protein of interest by indirect immunofluorescence. I have
raised antibodies (rabbit, guinea pig) to the cytoplasmic domain of a
neuronal transmembrane protein. These antibodies work well on
Westerns and in immunofluorescence of transfected COS cells (fixed
with 4% PFA in PBS, permeabilised with PBS + 0.1% Triton) However I
see little or nothing above background on sections of brain or retina.
The tissues are fixed overnight in 4% PFA prior to sectioning,
rehydrated in PBS and are permeabilised using 0.1% Triton before
blocking (PBS + 10% serum) and antibody addition. Antibodies to other
proteins, with various subcellular distributions, work well under these
conditions. I have tried post-fixing in 4% PFA without improvement. I
have not yet tried unfixed sections. If anyone has suggestions for
alternative fixation/permeabilisation/detection methods, or can direct me
to a good reference I would be very appreciative.


Thanks in advance,

rachel



Rachel K. Szilard, Ph.D.

Program in Developmental Biology
11-130 Elm Wing
Hospital for Sick Children
555 University Avenue
Toronto, Ontario
Canada
M5G 1X8

(416) 813 4997 phone
(416) 813 4931 fax
rszilard-at-sickkids.ca




From daemon Wed Jun 19 19:56:40 2002



From: Josh Kahn :      kahnj-at-biology.queensu.ca
Date: Wed, 19 Jun 2002 20:50:11 -0400 (EDT)
Subject: CLSM - Intermediate filament immunofluorescence of plant tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,

I'm looking for protocols for immunostaining intact (not protoplasts)
plant tissue intermediate filaments (using anti IFA, ME 101, or anything
else that will light the buggers up! Has anyone used anti-keratin
primaries?). Ideally for pea root nodules but anything else will help.

Thanks!



From daemon Wed Jun 19 22:08:03 2002



From: Dmrelion-at-aol.com
Date: Wed, 19 Jun 2002 23:00:09 EDT
Subject: immersion oil removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear list members,

One of my associates has a large number of thin sections of limestone that he
wants to look at with cathodoluminescence (CL). The problem is that the thin
sections have been coated already with immersion oil for other microscopy
observations and this may create problems with the vacuum and electron beam
in the CL apparatus. Does anyone have suggestions on the best/easiest way to
remove the immersion oil? The oil is identified as "Immersion oil for
Microscopy, Cargille Type A, Formula Code 1248" and is said to be made up of
synthetic hydrocarbons and natural petroleum derivatives.

He will appreciate any and all suggestions.

Thank you.

Don Marshall

RELION Industries
PO Box 12
Bedford, MA 01730

781-275-4695 (Phone)
781-271-0252 (FAX)

dmrelion-at-aol.com

cathodoluminescence, mass spectroscopy, electron beam technology

http://www.excitingelectrons.com


From daemon Wed Jun 19 23:40:09 2002



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thu, 20 Jun 2002 00:05:51 -0400
Subject: Philips EM420 IGP Power Supply

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Krzysztof, what's wrong with the IGP power supply? It is a very simple
circuit that is much easier and cheaper to repair than to ship overseas.
Components are available everywhere. Contact me off list with the detailed
description of the problem.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Krzysztof Herman {kherman-at-labsoft.com.pl}
To: MSA {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, June 19, 2002 4:26 PM


Hello List

Does somebody have the "IGP power supply" (or maybe some of it's components)
to sell cheap
or even donate to Czech microscopists ??

(I don't count on many offers ;-)) ... but to ask is allways worthwise..

regards

Krzysztof Herman
Labsoft, ul.Ba¿ancia 45A
02-892 Warszawa, Poland
tel/fx: (+48 22)6449753, 6449750
mobile: (+48 601)307456








From daemon Thu Jun 20 07:05:06 2002



From: JPMcCullough-at-fsl.gov.ie
Date: Thu, 20 Jun 2002 12:56:39 +0100
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Unsubscribe, please.



"The information transmitted is intended only for the person or entity to which it
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also mailminder-at-fsl.gov.ie".


From daemon Thu Jun 20 07:58:52 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Thu, 20 Jun 2002 10:22:07 -0230
Subject: RE: immersion oil removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Don Marshall writes ...

} One of my associates has a large number of thin sections of
} limestone that he wants to look at with cathodoluminescence
} (CL). The problem is that the thin sections have been coated
} already with immersion oil ...
} ... Does anyone have suggestions on the
} best/easiest way to remove the immersion oil? ...

Ethanol should work ... but I generally cut the difficult stuff with a
little acetone (in a hood) first, then an immediate immersion in ETOH.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com



From daemon Thu Jun 20 07:58:58 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Thu, 20 Jun 2002 08:52:49 -0400
Subject: Sputter target suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I only have two sources, Pella and Refining Systems. Who else is there??
Thanks

Scott Whittaker
SEM Lab Manager
Smithsonian Institution
PO Box 37012 MRC104
National Museum of Natural History
Washington DC 20013-7012
202-357-1651



From daemon Thu Jun 20 08:34:05 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 20 Jun 2002 09:26:12 -0400
Subject: Jet polishing recipe for stainless steel 316LN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a couple of recipes from the Philips monographs# 5 book (Thompson-Russell and Edington).

1) 10% Perchloric 90% acetic, 12v, 0C, jet polish
for austenitic SS:
2) 10% Perchloric, 20% glycerol, 70% ethanol, 65V electropolish
3)10% nitric, 90% water 50V, jet polish
4) 60% orthophosphoric, 40% sulphuric, 100C, no voltage specified, Jet (this works well for electropolishing too.)

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Yan Xin [mailto:xin-at-magnet.fsu.edu]
Sent: Wednesday, June 19, 2002 2:22 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

I need to make TEM samples from the bulk stainless steel.
I would appreciate very much if anyone could give me the recipe to make TEM
samples of stainless steel 316LN using jet polishing.

Thanks

Regards
Yan xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Thu Jun 20 10:21:57 2002



From: jtwilley-at-sprynet.com
Date: Thu, 20 Jun 2002 11:08:44 -0400
Subject: Re: immersion oil removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Don,

I have had good success using a very light petroleum distillate such as hexane
or "petroleum ether". This has very low surface tension (penetrates porosity
well) and a high evaporation rate so that it may be repeated several times in
quick succession. I would use a tissue to remove the excess immersion oil
first, followed by immersion in a minimal volume of the solvent - say 5ml for
a standard slide. Drain, allow to dry for a minute and then repeat with a
fresh 5ml volume. I have never seen observable residues in the SEM even from
structures such as clay stylolites inside biocalcarinites. I haven't tried CL
however, after this.

John Twilley
Conservation Scientist

On Wed, 19 Jun 2002 23:00:09 EDT "Dmrelion-at-aol.com"-at-sparc5.microscopy.com
wrote:

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear list members,

One of my associates has a large number of thin sections of limestone that he

wants to look at with cathodoluminescence (CL). The problem is that the thin
sections have been coated already with immersion oil for other microscopy
observations and this may create problems with the vacuum and electron beam
in the CL apparatus. Does anyone have suggestions on the best/easiest way to
remove the immersion oil? The oil is identified as "Immersion oil for
Microscopy, Cargille Type A, Formula Code 1248" and is said to be made up of
synthetic hydrocarbons and natural petroleum derivatives.

He will appreciate any and all suggestions.

Thank you.

Don Marshall

RELION Industries
PO Box 12
Bedford, MA 01730

781-275-4695 (Phone)
781-271-0252 (FAX)

dmrelion-at-aol.com

cathodoluminescence, mass spectroscopy, electron beam technology

http://www.excitingelectrons.com




From daemon Thu Jun 20 12:45:09 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 20 Jun 2002 13:38:06 -0400
Subject: RE: Jet polishing recipe for stainless steel 316LN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The recipes Scott gives would undoubtedly work, but there are significant
hazards, especially associated with Perchloric Acid. I would strongly urge
new users to discuss the hazards of this material with their Health and
Safety people before beginning work.

These hazards are not hypothetical. Several years ago we had a user
working with 20% Perchloric in Methanol (thankfully at -35C) in the
jet-polisher. For whatever reason (we have never found out) the solution
caught fire. Thankfully a clear-headed staff member was nearby and added
copious amounts of water, but the student was panicked, and frozen into
inaction. At higher temperatures this particular mixture is explosive!!!

We threw the jet polisher away and have not replaced it. All steel samples
in our lab now are made by ion-milling.

Tony

At 09:26 AM 6/20/2002 -0400, Walck, Scott D. wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
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From daemon Thu Jun 20 13:27:36 2002



From: jerzy.gazda-at-amd.com
Date: Thu, 20 Jun 2002 13:20:18 -0500
Subject: Jet polishing recipe for stainless steel 316LN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yan Xin,
working with perchloric acid can be a dangerous business, be careful not to let any salts accumulate on outside of your solution bottles, cool the stuff while mixing, and do not store used solutions for extended period of time. Salts of perchloric acid can be explosive or spontaneously combust. Also in USA OSHA requires special hoods with water cleanup for work with perchloric acid.

Alternatively, I have a recipe from a guide book published years ago by Bernie Kestel at ANL. The reference for this publication is ANL-80-120 revised edition from 1986.

Using jet electro polisher (single polisher was used to acquire this data):

solution of:
20% H2SO4 + 80% Methanol at 20°C with medium stir rate, 20V, 320-400 mA gives removal rate of ~300nm / sec when fresh or 200nm when re-used.

Personally, I like to mix these components while holding my container in a cooling bath of methanol and dry ice (solid CO2), they heat up and could be flammable when combined too fast. I used this solution extensively with SS316 and vanadium alloys (irradiated and annealed materials) and found that mixing the two types of specimens did not cause any problems, as mixed solution has about one month shelf life due to its hygroscopic nature. Cooling the solution below 0°C allows better control of removal rates.


Have fun and be careful to work in a vented hood.

Jerzy
******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************







-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Thursday, June 20, 2002 8:26 AM
To: 'Yan Xin'
Cc: Microscopy (E-mail)


I have a couple of recipes from the Philips monographs# 5 book (Thompson-Russell and Edington).

1) 10% Perchloric 90% acetic, 12v, 0C, jet polish
for austenitic SS:
2) 10% Perchloric, 20% glycerol, 70% ethanol, 65V electropolish
3)10% nitric, 90% water 50V, jet polish
4) 60% orthophosphoric, 40% sulphuric, 100C, no voltage specified, Jet (this works well for electropolishing too.)

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Yan Xin [mailto:xin-at-magnet.fsu.edu]
Sent: Wednesday, June 19, 2002 2:22 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

I need to make TEM samples from the bulk stainless steel.
I would appreciate very much if anyone could give me the recipe to make TEM
samples of stainless steel 316LN using jet polishing.

Thanks

Regards
Yan xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================







From daemon Thu Jun 20 14:34:27 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Thu, 20 Jun 2002 16:43:42 -0400
Subject: Immersion oil removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I know this is a little "off list", but does anyone out there have
any any strong opinions (oh boy, am I asking for it) about xylene vs
d-Limonene as a clearing solvent for paraffin processing? We
switched to limonene a little while ago, because of its lower
toxicity, but have had problems with adequate infiltration and
crunchy tissues, especially liver.
thanks in advance. You can respond on or off list,
Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


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Dear List:

I have a nice late 1950's vintage Wild M-11 light microscope. The
coarse focus mechanism is shot, the teeth on the two pieces that
interact are beyond repair. There don't seem to be any spare parts
available, even from Heerbrugg, Switzerland. I am looking for someone
with parts for this microscope or another M-11 with a different
unrepairable defect that I can cannibalize for parts.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




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Kia Ora Scott,

We send our sputter coater targets for recoating to

Testbourne Ltd
Unit 12/13, Hassocks Wood,
Stroudley Road
Basingstoke
Hampshire,
England
RG24 8UQ

Phone ++44 (0) 1256 467055
Fax ++44 (0) 1256 842929

email: info-at-testbourne.com


Good turn around time (and we are on the other side of the world),
good price, they give us a refund on any metal scraps left on the
target, and they send us a christmas card at Christmas...... what
more could you ask for.


Allan




At 8:52 AM -0400 20/6/02, Scott Whittaker wrote:
} I only have two sources, Pella and Refining Systems. Who else is there??
} Thanks
}
} Scott Whittaker
} SEM Lab Manager
} Smithsonian Institution
} PO Box 37012 MRC104
} National Museum of Natural History
} Washington DC 20013-7012
} 202-357-1651
--
-------------------------------------------------
Allan Mitchell
Technical Manager
Otago Centre for Electron Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


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Don, I'm digging way back in the gray matter for this one. I believe it was
Ladd Ind. who sold a reflux unit specifically for the purpose of washing TEM
replicas in the days before SEM. It consisted of a water cooled probe which
supported the grid on a fine wire mesh. This probe was inserted into the
reflux unit where the heated solvent vapors would continually wash the grid.
You could probably build a similar setup and use Hexane to wash out the oil.


Russ Gillmeister
Microscopy
Xerox Corp.
Webster, NY 14580




From daemon Thu Jun 20 17:12:08 2002



From: Michael Calabrese :      mcalabrese-at-rwsc.com
Date: Thu, 20 Jun 2002 16:08:15 -0700
Subject: RE: Jet polishing recipe for stainless steel 316LN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Energy Beam Sciences.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (800) 992-9037
www.ebsciences.com
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: Scott Whittaker [mailto:Whittaker.scott-at-nmnh.si.edu]
Sent: Thursday, June 20, 2002 8:53 AM
To: microscopy-at-microscopy.com


I only have two sources, Pella and Refining Systems. Who else is there??
Thanks

Scott Whittaker
SEM Lab Manager
Smithsonian Institution
PO Box 37012 MRC104
National Museum of Natural History
Washington DC 20013-7012
202-357-1651





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I have used the following solutions for jet polishing(Tenupol) various
stainless steels and Ni based alloys:
a) 125ml methanol, 75ml butyl alcohol, 20ml perchloric acid -at-
-35C/16V

b) 150ml methanol, 50ml nitric acid -at- -40C/15V

They both work well. There are dangers if you heat these solutuions. Cool
the methanol before adding the acid. Keep them cool when using and do not
store. Dilute them with water when transfering to a disposal container.
(Don't catch them on fire!)

Mike Calabrese
mcalabrese-at-rwsc.com



From daemon Thu Jun 20 18:54:52 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 20 Jun 2002 21:32:44 -0500
Subject: Removal of Cargille Type A, Formula Code 1248

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try:

Refining Systems, Inc.
Las Vegas, NV
702-368-0579.


gg


} At 8:52 AM -0400 20/6/02, Scott Whittaker wrote:
} } I only have two sources, Pella and Refining Systems. Who else is there??
} } Thanks
} }
} } Scott Whittaker
} } SEM Lab Manager
} } Smithsonian Institution
} } PO Box 37012 MRC104
} } National Museum of Natural History
} } Washington DC 20013-7012
} } 202-357-1651



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To: MICROSCOPY BB {Microscopy-at-sparc5.microscopy.com}


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Don Marshall wrote:
=============================================================
One of my associates has a large number of thin sections of limestone that
he
wants to look at with cathodoluminescence (CL). The problem is that the thin
sections have been coated already with immersion oil for other microscopy
observations and this may create problems with the vacuum and electron beam
in the CL apparatus. Does anyone have suggestions on the best/easiest way to
remove the immersion oil? The oil is identified as "Immersion oil for
Microscopy, Cargille Type A, Formula Code 1248" and is said to be made up of
synthetic hydrocarbons and natural petroleum derivatives.

He will appreciate any and all suggestions.
=============================================================
The manufacturer of Cargille fluids told me today that the "recommended"
solvents would be heptane, tolune, xylene, or ethyl ether. Interestingly,
they said acetone would not do as good of a job as these other solvents.

Disclaimer: SPI Supplies distributes the full range of fluids from Cargille
for light microscopy applications.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Thu Jun 20 22:57:59 2002



From: Frank Eugene Jones :      jonesfe-at-darkwing.uoregon.edu
Date: Thu, 20 Jun 2002 20:46:41 -0700
Subject: Re: Sputter target suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

Try the Kurt J. Lesker company. I see on their web site
(www.kurtlesker.com) that they are having a sale on targets.

Frank
}
----------------------------------------------------------------------
--
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------
-.
}
}
} I only have two sources, Pella and Refining Systems. Who else is
there??
} Thanks
}
} Scott Whittaker
} SEM Lab Manager
} Smithsonian Institution
} PO Box 37012 MRC104
} National Museum of Natural History
} Washington DC 20013-7012
} 202-357-1651
}
}
*******************************************************
Frank E. Jones Physics Graduate Student
Lonergan Lab (Chemistry) University of Oregon
Office: 103a Klamath Phone: (541)346-2900
or 346-0977
email: jonesfe-at-darkwing.uoregon.edu
homepage: darkwing.uoregon.edu/~jonesfe


From daemon Fri Jun 21 01:02:19 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Fri, 21 Jun 2002 07:52:28 +0200
Subject: RE: immersion oil removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Don

I am passing on a bit of advice a service technician give me to clean
microscope slides from immersion oil. This may work for your sample as
well. Normally I just rinse slides with it. Since your sample is more
robust the following may work. :



Place the with the exposed side down for approx 2 minutes in a mixture of
10 % Acetone
20 % Chloroform
70 % Absolute Ethanol

You can use a hair drier to speed up the evaporation




Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana

Phone : +267 355 2426
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw


} -----Original Message-----
} From: "Dmrelion-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Dmrelion-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Thursday, June 20, 2002 5:00 AM
} To: microscopy-at-microscopy.com
} Subject: immersion oil removal
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear list members,
}
} One of my associates has a large number of thin sections of
} limestone that he
} wants to look at with cathodoluminescence (CL). The problem
} is that the thin
} sections have been coated already with immersion oil for
} other microscopy
} observations and this may create problems with the vacuum and
} electron beam
} in the CL apparatus. Does anyone have suggestions on the
} best/easiest way to
} remove the immersion oil? The oil is identified as
} "Immersion oil for
} Microscopy, Cargille Type A, Formula Code 1248" and is said
} to be made up of
} synthetic hydrocarbons and natural petroleum derivatives.
}
} He will appreciate any and all suggestions.
}
} Thank you.
}
} Don Marshall
}
} RELION Industries
} PO Box 12
} Bedford, MA 01730
}
} 781-275-4695 (Phone)
} 781-271-0252 (FAX)
}
} dmrelion-at-aol.com
}
} cathodoluminescence, mass spectroscopy, electron beam technology
}
} http://www.excitingelectrons.com
}


From daemon Fri Jun 21 01:06:54 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Fri, 21 Jun 2002 07:58:59 +0200
Subject: RE: Sputter target suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Scott

I do not know how limited your fund are. But in the past we just buy 24
Carat gold in sheet form from the reserve bank. There is lot of additional
effort in getting permits etc. Then if you have a polaron which uses a ring
rather than a circle like in the SPI coater you still have to cut it
yourself which will kneed help from a workshop. The end result was that we
were able to get double the sputter target thickness for half the price. A
chat to a local jeweller will help.






Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana

Phone : +267 355 2426
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw


} -----Original Message-----
} From: Scott Whittaker [mailto:Whittaker.scott-at-nmnh.si.edu]
} Sent: Thursday, June 20, 2002 2:53 PM
} To: microscopy-at-microscopy.com
} Subject: Sputter target suppliers
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} I only have two sources, Pella and Refining Systems. Who else
} is there??
} Thanks
}
} Scott Whittaker
} SEM Lab Manager
} Smithsonian Institution
} PO Box 37012 MRC104
} National Museum of Natural History
} Washington DC 20013-7012
} 202-357-1651
}
}


From daemon Fri Jun 21 07:50:01 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Fri, 21 Jun 2002 08:42:34 -0400
Subject: Re: Sputter Coater Targets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hello all, does anyone out there know how to make up ammonium carbonate buffer
to pH 7.0.
thanks
john


From daemon Fri Jun 21 12:11:21 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Thu, 20 Jun 2002 12:59:13 -0500
Subject: Olympus DP12 digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I am considering buying an Olympus DP12 digital camera for light microscopy use and I am looking for advice on how this
camera compares with other cameras on the market from Nikon, Kodak, Zeiss etc.

I want something that is a good value now in the face of rising megapixels and falling prices. Is its 3.34 MP chip enough for
high quality images?

I would appreciate your comments because I have no experience with digital photography.

Greg




From daemon Fri Jun 21 14:57:10 2002



From: Andreas Taubert :      taubert-at-seas.upenn.edu
Date: Fri, 21 Jun 2002 15:48:52 -0700
Subject: IR microscope manufacturers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

we are looking into buying an IR microscope and I am currently trying to
assemble a list of vendors. I am aware of Thermo Nicolet, Omicron, and
Perkin Elmer. Any other sources ? We are looking for an intrument that can
do microspectroscopy, i.e., spatially resolved IR spectroscopy on ~ 1 micron
(or better, of course) length scale.

Thanks in advance
Andreas

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Andreas Taubert
Dept. of Materials Science & Engineering
3231 Walnut St.
University of Pennsylvania
Philadelphia PA 19104-6272
Tel.: 215 573 1179
Fax: 215 573 2128
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Fri Jun 21 15:24:32 2002



From: Andreas Taubert :      taubert-at-seas.upenn.edu
Date: Fri, 21 Jun 2002 16:17:49 -0700
Subject: IR microscope manufacturers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

we are looking into buying an IR microscope and I am currently trying to
assemble a list of vendors. I am aware of Thermo Nicolet, Omicron, and
Perkin Elmer. Any other sources ? We are looking for an intrument that can
do microspectroscopy, i.e., spatially resolved IR spectroscopy on ~ 1 micron
(or better, of course) length scale.

Thanks in advance
Andreas

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Andreas Taubert
Dept. of Materials Science & Engineering
3231 Walnut St.
University of Pennsylvania
Philadelphia PA 19104-6272
Tel.: 215 573 1179
Fax: 215 573 2128
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Fri Jun 21 15:31:28 2002



From: =?iso-8859-1?Q?Jos=E9?= Reinaldo Guerrero :      guerrero-at-ivic.ve
Date: Fri, 31 May 2002 18:05:48 -0400
Subject: subscription

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir

Could you subscribe me at the microscopy club?

Thanks

José Reinaldo Guerrero





From daemon Fri Jun 21 17:13:15 2002



From: Mary K. O'Connell :      oconne1l-at-leland.stanford.edu
Date: Fri, 21 Jun 2002 15:05:11 -0700
Subject: Measurement of Collagen Fiber Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Members:

I am struggling to measure the orientation of collagen fibers in the layers
of a blood vessel. In healthy aorta, I expect to see a semi-organized
arrangement of predominantly helically-oriented fibers, as well as a few
longitudinally-oriented fibers.

Thus far, have investigated polarized light microscopy (PLM) and confocal
laser scanning microscopy (CLSM). Initial efforts with CLSM revealed poor
collagen visibility (trying to improve staining technique) and difficulties
in imaging through the thickness of the vessel (0.2mm). PLM equipment was
limited and was difficult to correlate orientation from one layer to the
next.

I would greatly appreciate any input on possible techniques or resources
for measuring fiber orientation. Thank You!

Mary


***************************************************
Mary K.O'Connell
Cardiovascular Biomechanics Research Lab
MSLS: Room P224 - Surgery
1201 Welch Road
Stanford, CA 94305
(650) 723-1695
(650) 498-6262 Fax
E-mail: oconne1l-at-leland.stanford.edu
(Note: O'Connell in the E-mail address is spelled with a numeric "1" for
the first l, and an alphabetic "l" for the second.)
*******************************************************


From daemon Fri Jun 21 17:18:07 2002



From: Mary K. O'Connell :      oconne1l-at-leland.stanford.edu
Date: Fri, 21 Jun 2002 15:12:09 -0700
Subject: Measurement of Collagen Fiber Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Members:

I am struggling to measure the orientation of collagen fibers in the layers
of a blood vessel. In healthy aorta, I expect to see a semi-organized
arrangement of predominantly helically-oriented fibers, as well as a few
longitudinally-oriented fibers.

Thus far, have investigated polarized light microscopy (PLM) and confocal
laser scanning microscopy (CLSM). Initial efforts with CLSM revealed poor
collagen visibility (trying to improve staining technique) and difficulties
in imaging through the thickness of the vessel (0.2mm). PLM equipment was
limited and was difficult to correlate orientation from one layer to the
next.

I would greatly appreciate any input on possible techniques or resources
for measuring fiber orientation. Thank You!

Mary


***************************************************
Mary K.O'Connell
Cardiovascular Biomechanics Research Lab
MSLS: Room P224 - Surgery
1201 Welch Road
Stanford, CA 94305
(650) 723-1695
(650) 498-6262 Fax
E-mail: oconne1l-at-leland.stanford.edu
(Note: O'Connell in the E-mail address is spelled with a numeric "1" for
the first l, and an alphabetic "l" for the second.)
*******************************************************


From daemon Fri Jun 21 18:03:28 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 21 Jun 2002 15:55:13 -0400
Subject: Re: Ammonium carbonate

Contents Retrieved from Microscopy Listserver Archives
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on 6/21/02 8:42 AM, John Hoffpauir at John.Hoffpauir-at-mail.tju.edu wrote:
}
} hello all, does anyone out there know how to make up ammonium carbonate buffer
} to pH 7.0.
} thanks
} john
}
Dear John,
Ammonium bicarbonate (NH4HCO3) is pretty close to pH 7.0. If it is
below that pH, just add (NH4)2CO3; if it is above that pH, just bubble CO2
through the solution.
Yours,
Bill Tivol



From daemon Fri Jun 21 20:14:57 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Thu, 20 Jun 2002 21:03:55 -0500
Subject: Re: LM: need help fixing + embedding sugarcane

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Hello Gabrielle,

Young sugarcane internodes (cross sections abt 1-1.5 cm diam) and stem apices (longitudinal sections) fix fine in an
FAA-TBA-Ethanol series we have been using at the University of the West Indies in Trinidad, forever and ever. If you
send me some more details of your methods I will ask my technician next week, and I can send you our fixation
schedule, which is as old as the hills.

Greg


} Does anyone have any experience with fixing and embedding
} sugarcane stem tissue for in situ hybridization? So far we have
} tried a number of protocols - including traditional fixation in 4%
} paraformaldehyde, as well as prefixing and then freezing in
} isopentane and cutting frozen sections - but to no avail. With
} traditional chemical fixation (a protocol which worked beautifully for
} one of our colleagues working on grape berries), we have to fix for 5-
} 7 days just to get adequate fixation, and even then parts of the
} older internode tissue don't fix properly - this makes sectioning very
} tricky. With cutting prefixed frozen tissue, our sections break up
} very easily, and even when we do get sections, once thawed they
} go into some sort of shock and develop thick black cell borders
} (even in the xylem).
}
} If anyone has any ideas, please let me know!
}
} Thanks
} Gabrielle
}
}
}
} Gabrielle Turner
} Institute for Plant Biotechnology
} University of Stellenbosch
} 7600
}
} Cell: 083 324 7453
}





From daemon Fri Jun 21 20:32:28 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Thu, 20 Jun 2002 21:23:31 -0500
Subject: Re: Need help fixing and embedding Sugarcane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello Gabrielle,

Young sugarcane internodes (cross sections abt 1-1.5 cm diam) and whole stem apices section great after fixation in an
FAA-TBA-Ethanol series we have been using at the University of the West Indies in Trinidad forever.

Send me some more details of your methods and I will ask my technician next week to check your schedule. I can send
you our schedule which is as old as the hills.

Greg





From daemon Fri Jun 21 20:51:05 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Thu, 20 Jun 2002 21:41:48 -0500
Subject: Reply to Need help fixing and embedding Sugarcane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello Gabrielle,
}
} Young sugarcane internodes (cross sections abt 1-1.5 cm diam) and whole stem apices section great in an
} FAA-TBA-Ethanol series we have been using at the University of the West Indies in Trinidad.
}
} Send me some more details of your methods and I will ask my technician next week to look at them. I can send you our
schedule, which is as old as the hills.
}
} Greg




From daemon Sat Jun 22 19:38:52 2002



From: DrJohnRuss-at-aol.com
Date: Sun, 23 Jun 2002 07:29:51 EDT
Subject: Re: Measurement of Collagen Fiber Orientation

Contents Retrieved from Microscopy Listserver Archives
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Mary,

I was using my BioRad MRC1000 CLSM) to look at sections of some cells grown
in collagen matrix and stained with H&E. I was amazed that the collagen
really lit up as did the cells but not the nuclei. These were cross section
of rat aorta and I was looking at the endothelial cells growing out to form
accessory capillaries? The sections were about 30-50 micrometers thick.

As far a orientation, Fovea Pro 2.0 sold by Reindeer Games can do that with
a digital image; I'm not sure about a stack of images. Check with Dr. John
Russ, he can advise you better; he'll probably answer your email anyway.
Usual disclaimer - no financial interest, etc. just a user.

Damian

Damian Neuberger, Ph.D
Senior Research Scientist
Baxter Healthcare Corp.

----- Original Message -----
} From: "Mary K. O'Connell" {oconne1l-at-leland.stanford.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, June 21, 2002 5:12 PM



In a message dated 6/22/02 8:51:23 PM, neuberger1234-at-attbi.com writes:

} As far a orientation, Fovea Pro 2.0 sold by Reindeer Games can do that
} with a digital image; I'm not sure about a stack of images. Check with Dr.
} John Russ, he can advise you better; he'll probably answer your email anyway.

Well, I guess I'd better answer it now. I have no advice on sample prep or
getting the image in the first place, but if you have representative images
of the structure the procedure for determining orientation is pretty
straightforward, and yes, Fovea Pro (http://reindeergraphics.com) does
include that function. At each point in the image, the vector of brightness
gradient points perpendicular to the orientation of the collagen fiber.
Calculating that angle and assigning the value as a grey scale to the pixel
is the first step. If there are points in the image that are not collagen
fibers, a Boolean operation is then used to eliminate them. Finally, the
histogram of the grey scale (orientation) values is summed and plotted as a
rose plot to show the preferred orientation.

John Russ


From daemon Sun Jun 23 08:56:49 2002



From: Brendan J. Griffin :      bjg-at-cyllene.uwa.edu.au (by way of
Date: Sun, 23 Jun 2002 08:44:57 -0500
Subject: Lecturer Position: Centre for Microscopy & Microanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



LECTURER (REF: A26/20)
CENTRE FOR MICROSCOPY AND MICROANALYSIS
The Centre for Microscopy and Microanalysis (CMM) is the principal
node of the Western Australian Centre for Microscopy (WACM), a State
Government Centre of Excellence at the University of Western
Australia. WACM has partner facilities at Curtin, Murdoch and Edith
Cowan Universities. The CMM is also a core partner in the recently
established Nanostructural Analysis Network Organisation (NANO) and
we expect to install a high-resolution ion microprobe in 2003, as
part of the local NANO capability. CMM facilities are extensive, and
include digital optical and confocal microscopes, four TEMs and seven
SEMs, together with extensive specimen preparation equipment in a
newly refurbished site.

The appointee(s) will provide leadership and collaboration with users
in biological research involving optical, confocal and electron
microscopy techniques. In particular, the appointee will be expected
to initiate the development and enhancement of cryo-microscopy, x-ray
microanalysis and associated specimen preparation. Activities will
include the training and teaching of users of microscopical and
related microanalysis facilities in biological applications. It is
essential that the appointee has detailed knowledge in the
instrumental and application aspects of biological microscopy.
Experience with or an interest in relevant SIMS techniques would be
of benefit and should be noted in the application.

The position is expected to be formally shared with one or more of
the newly-formed UWA Schools of Plant Biology, Biomedical and
Chemical Sciences, Animal Biology or Anatomy and Human Biology.

The minimum qualification required is a PhD in Biological/Biomedical
Sciences or equivalent. The successful applicant must have strong
interpersonal skills and the ability to work with other staff within
the Centre and its users. Joint supervision of post-graduate students
is expected, in the relevant Schools. The position is tenurable.
Applicants with tertiary teaching experience should submit a teaching
portfolio as part of their application.

For further information and copies of the selection criteria please
contact the Director of the CMM, Associate Professor Brendan Griffin,
on telephone (08) 9380 2770 or email director-at-cmm.uwa.edu.au.
Details of the CMM may be found at: http://cmm.uwa.edu.au

SALARY RANGE: Lecturer Level B $53,592 - $63,642 p.a. (AUD)

CLOSING DATE: 28th June 2002

Late applications will continue to be received after the closing date
and may be considered until the position is filled. The University
reserves the right to not make an appointment to this position or to
fill the position at a different level.

Located adjacent to the picturesque banks of the Swan River, the
University of Western Australia offers an attractive benefits package
including generous superannuation, fares to Perth (if applicable) for
appointee and dependants along with a removals allowance, generous
leave provisions and a working environment that's the envy of many.
These and other benefits will be specified in the offer of employment.

APPLICATION DETAILS: Written applications quoting the reference
number, personal contact details, qualifications and experience,
along with contact detail of three referees should be sent to
Director, Human Resources, The University of Western Australia, 35
Stirling Highway, Crawley WA 6009 by the closing date.

Advertised internationally.

--




Brendan J. Griffin
Director & Assoc. Professor in Electron Microscopy
Centre for Microscopy and Microanalysis
The University of Western Australia
First floor, Physics Building,
35 Stirling Highway
CRAWLEY, WA, AUSTRALIA 6009
ph 61-8-9380-2739 fax 9380-1087
mobile 0409-104-096
bjg-at-cmm.uwa.edu.au
http://cmm.uwa.edu.au/

CRICOS Provider No. 00126G


From daemon Sun Jun 23 16:03:55 2002



From: Pauline C. Yu :      pcy-at-usc.edu
Date: Sun, 23 Jun 2002 13:55:44 -0700 (PDT)
Subject: light microscopy and RSIs--motorized stages?

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,

I am currently experiencing possible symptoms of carpel tunnel syndrome
after having spent much of this summer at the helm of a microscope, and
this is in addition to the mild tendonitis I contracted from computer use
a few years ago. Does any one know about add-on motors for stages that
allow foot pedal control? I feel like this is a more viable solution than
buying a new scope with all the bells and whistles(besides the fact that
I'm only a graduate student and the budget decisions are not mine to
make!)
Of course any other suggestions from people with similar problems would be
greatly appreciated.
Thanks in advance,

Pauline Yu
pcy-at-usc.edu
Manahan Lab
http://www.usc.edu/manahanlab



From daemon Sun Jun 23 17:07:24 2002



From: msandusk-at-bucknell.edu (by way of MicroscopyListserver)
Date: Sun, 23 Jun 2002 16:56:39 -0500
Subject: Ask-A-Microscopist: immunostain & shrinkage question

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (msandusk-at-bucknell.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on Sunday,
June 23, 2002 at 14:45:43
---------------------------------------------------------------------------

Email: msandusk-at-bucknell.edu
Name: Matt Sandusky

Organization: Bucknell Univeristy

Education: Undergraduate College

Location: Lewisburg, Pennsylvania, USA

Question: I'm working on an immunostain of 10 micron sections
of rat testis and I'm having trouble with shrinkage.
The seminiferous tubules are shrinking away from the
interstitial tissue. I'm currently working on perfusion
fixation using 4% paraformaldehyde and a .01M phosphate
buffer. Once fixed, I slice each testis in half and
embed in paraffin using 30min PBS, 50%EtOH for 1 hr,
70%EtOH 1hr, 90%EtOH 1hr, 3 of 100%EtOH 2hrs, 3 of
xylene for 1hr 30min, 2hr paraffin and 3hr paraffin (with vaccum).
Any advice anyone might be able to offer would be
greatly appreciated. Thanks.

---------------------------------------------------------------------------


From daemon Mon Jun 24 06:08:27 2002



From: Sampath, Srinidhi (CORP, GEITC) :      Srinidhi.Sampath-at-geind.ge.com
Date: Mon, 24 Jun 2002 16:20:56 +0530
Subject: 3D image analysis...

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Does anyone have an idea of how to do 3D image analysis for accurate
interparticle spacing on TEM images (projected images). Is there a
corresponding formula to:
lambda = d(particle)*f(matrix)/f(particle)
where lambda = interparticle spacing
d(particle) = dia of particle
f(matrix) = volume fraction of matrix
f(particle) = volume fraction of particle

Thanks,
Srinidhi
srinidhi.sampath-at-geind.ge.com


"THIS E-MAIL MESSAGE ALONG WITH ANY ATTACHMENTS IS INTENDED ONLY FOR THE
ADDRESSEE and may contain confidential and privileged information.
If the reader of this message is not the intended recipient,
you are notified that any dissemination, distribution or copy of this
communication is strictly Prohibited.
If you have received this message by error, please notify us
immediately, return the original mail to the sender and delete the
message from your system."



From daemon Mon Jun 24 07:37:23 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 24 Jun 2002 08:33:35 -0400
Subject: IR microscope manufacturers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Barnes also for thermal imaging. What wavelength do you need to work
at?

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Andreas Taubert [mailto:taubert-at-seas.upenn.edu]
Sent: Friday, June 21, 2002 6:49 PM
To: Microscopy List Server


Dear Listers,

we are looking into buying an IR microscope and I am currently trying to
assemble a list of vendors. I am aware of Thermo Nicolet, Omicron, and
Perkin Elmer. Any other sources ? We are looking for an intrument that can
do microspectroscopy, i.e., spatially resolved IR spectroscopy on ~ 1 micron
(or better, of course) length scale.

Thanks in advance
Andreas

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Andreas Taubert
Dept. of Materials Science & Engineering
3231 Walnut St.
University of Pennsylvania
Philadelphia PA 19104-6272
Tel.: 215 573 1179
Fax: 215 573 2128
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Mon Jun 24 10:32:57 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Mon, 24 Jun 2002 11:21:02 -0400
Subject: TEM POSITION

Contents Retrieved from Microscopy Listserver Archives
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Transmission Electron Microscopist

Princeton University's Department of Molecular Biology is seeking a
Transmission Electron Microscopist to run a new LEO912AB. Duties will
include: (1) teaching and training undergraduate and graduate students,
post-docs and faculty on instrument operation, (2) preparation and
sectioning of diverse biological specimens including yeast, virally infected
mammalian cells, brain slices and Drosophila embryos, using a variety of
methods including high-pressure freezing and freeze substitution techniques,
(3) daily microscope system checks and scheduling of users, (4) general lab
maintenance and supply. The candidate should have a BS/MS in a biological
field and several years of TEM experience. Knowledge of ultra-structural
and immuno-labeling protocols, the operation and care of microtomes and
other equipment needed for specimen preparation, a basic understanding of
digital imaging and the ability to manage and administer digital
workstations and image archives is essential. The candidate must be highly
interactive, willing to collaborate on diverse projects and able to identify
and research the best methods of specimen preparation and examination. Rank
and salary are dependent upon qualifications and experience. Please send
curriculum vitae, a list of references and representative samples of your
work to Professor Mark Rose, Chair, Search Committee, Department of
Molecular Biology, Princeton University, Princeton, NJ 08544-1014.
Princeton University is an Equal Opportunity/Affirmative Action Employer



Joe Goodhouse
Confocal / EM Core Lab Manager
Department of Molecular Biology
Princeton University
jgoodhouse-at-molbio.princeton.edu
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Mon Jun 24 10:54:34 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Mon, 24 Jun 2002 11:44:42 -0400
Subject: ignore-2

Contents Retrieved from Microscopy Listserver Archives
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Thank you too.


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432



From daemon Mon Jun 24 11:18:01 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Mon, 24 Jun 2002 12:08:03 -0400
Subject: ignore test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you.

Joe Goodhouse
Confocal / EM Core Lab Manager
Department of Molecular Biology
Princeton University
jgoodhouse-at-molbio.princeton.edu
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Mon Jun 24 11:22:25 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Mon, 24 Jun 2002 12:13:09 -0400
Subject: ignore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you

Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University



From daemon Mon Jun 24 15:51:57 2002



From: Jolanta Jacobs :      jolanta-at-ksu.edu
Date: Mon, 24 Jun 2002 16:01:21 -0500
Subject: LM chloral hydrate in Alexander's stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everybody,
I need to do a vital stain on pollen of Arabidopsis mutant I'm working on-it
seems Alexander's stain is still widely used, at least outside US. One of
the ingredients is chloral hydrate which happens to be a schedule IV
substance. I called DEA to get the license but it may take longer than I
have the time for. Is there a substance that may be used instead of chloral
hydrate? Or some other as easy as Alexander's vital staining method for
pollen?
Thank you for any input you may have,
Jolanta Jacobs
Grad student in Div of Biology
Manhattan, Kansas



From daemon Mon Jun 24 15:54:59 2002



From: Chun-Ming Li :      lichun-at-uiuc.edu
Date: Mon, 24 Jun 2002 15:48:14 -0500
Subject: Re: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear friends,

Thank you very much for your useful suggestions on polishing multilayers
on glass! I will try them.

Best regards,
Chunming Li

Sousan Abolhassani wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Chun-Ming,
}
} As your multi layer is thick, in any case you should thin it
} afterwards.
} Instead of going through the problem of dissolving and collecting
} such a layer that you still need to thin by other techniques
} there are at least three possibilities (that do not need suffisticated
} facilities):
}
} a. If you want to see the top view of your multi-layer, then
} it means that you should thin your sample from the molybdenum
} side until it is electron transparent. For this, the sample
} should be grinded from the glass side to a thickness of the order
} of 20-50 microns, then mounted on a ring sample holder used
} for ion milling. (If you can grind down to 30 micrometers it needs a
} shorter time for ion-milling, but if you cann't it is not important).
} Then you ion-mill your sample from the glass side, until you see a hole
} and you know that the thinnest part is in the CuInSe2 region.
}
} Note that in this case you will not see the molybdenum, because your
} CuInSe2 is 0.7-2.5 micrometers as you say.
} If you wish to observe the molybdenum as well, you could take another
} sample and proceed as above, but stop milling when the sample is
} near the Mo (you could see from colour changes) and then ion mill
} from the other side.
}
} b. The second possibility is to do a tripod polishing and this can
} be done with different orientations. All layers will be observable
} and from my experience this is the most convenient method, that
} does not need to complicated facilities.
}
} c. The last method, that I use for quick determinations of the
} material before the above elaborate methods, is to scratch the
} surface to be observed by a diamond pointer and then put the
} sctratched debris in a solvent that does not attack the film
} (you should use a very small recipient not to loose your material)
} and collect the debris on a grid with a thin support film.
} This method is very rapid and can quickly bring some information
} before you proceed with more elaborate techniques.
}
} Hope this helps,
}
} Sousan
}
} E-mail: sousan.abolhassani-at-psi.ch
} http://www.psi.ch
}
} Chun-Ming Li wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Dear friends:
} } I have a problem in preparing a TEM sample (plan view). The
} } as-received sample is as follow:
} } On a soda-lime glass (thickness 2-4 mm) substrate, deposit a
} } molybdenum layer (thickness 0.1-1.5 micron), and then deposit a layer of
} } CuInSe2 (thickness 0.7-2.5 micron).
} } After dissolved the glass with HF (hydrofluoric) acid (cover the layers
} } with lacquer before etching, otherwise the multilayers will also be
} } dissolved), I immersed the multilayers into acetone. But the layers are
} } too brittle that they broke into tiny chips. Even sometimes I can get a
} } big chip, it is still very difficult to fix it on a grid, since it is
} } too brittle.
} } I would appreciate it very much if you can give me any suggestions
} } to solve this problem, i.e., how to get a plan-view TEM sample from the
} } as-received sample. The major problem would be how to get rid of the
} } glass and fix the multilayers to grid before ion milling.
} } Thank you in advance!
} }
} } Chun-Ming Li, Ph.D.
} } Dept. of Mater. Sci. and Engi.
} } University of Illinois at Urbana-Champaign



From daemon Mon Jun 24 17:07:50 2002



From: Jolanta Jacobs :      jolanta-at-ksu.edu
Date: Mon, 24 Jun 2002 17:18:22 -0500
Subject: LM chloral hydrate in Alexander's stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everybody,
I need to do a vital stain on pollen of Arabidopsis mutant I'm working on-it
seems Alexander's stain is still widely used, at least outside US. One of
the ingredients is chloral hydrate which happens to be a schedule IV
substance. I called DEA to get the license but it may take longer than I
have the time for. Is there a substance that may be used instead of chloral
hydrate? Or some other as easy as Alexander's vital staining method for
pollen?
Thank you for any input you may have,
Jolanta Jacobs
Grad student in Div of Biology
Manhattan, Kansas



From daemon Mon Jun 24 20:30:03 2002



From: Paul Fischione :      pe_fischione-at-fischione.com (by way of
Date: Mon, 24 Jun 2002 20:20:38 -0500
Subject: Microscopy Society of America (MSA) Needs You

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: Microscopy Society of America: Marketing and Communications Committee
(MSAMCC)

Colleagues:

MSA is a vibrant society which encompasses all facets of Microscopy
and Microanalysis. The society and it's members are dedicated to the
promotion and advancement of the knowledge of the science and practice
of all microscopical imaging, analysis and diffraction techniques useful
for elucidating the ultrastructure and function of materials in diverse
areas of biological, materials, medical and physical sciences. To this end
MSA is a resource which is second to none throughout the world. If you're
not currently a member of MSA, then you're missing out on both the
opportunities and the fun; and we would like to take this opportunity to
invite you to join our family.


If you are already an MSA member, take a moment to talk to your colleagues
and/or students about MSA's great benefits and encourage them to join the
society. Invite them to be come part of our world wide microscopy community.


MSA Membership Benefits Include:

* A subscription to the high-quality journal: Microscopy & Microanalysis
-included with your membership dues
http://www.msa.microscopy.com/MM/MscopyManalysis.html
* A complimentary copy of Microscopy Today
http://microscopy-today.com
* Receive advanced information and reduced fees for the Annual Meeting
- the preeminent microscopy related meeting and exhibition
http://www.microscopy.com/MSAMeetings/MMMeeting.html
* Access to extensive educational materials & activities
http://www.msa.microscopy.com/RefEdu.html
* On-line Directory of MSA members
http://www.msa.microscopy.com/MSAMembers/SearchMembersDB.html
* Opportunities to have your accomplishments recognized as an Awards recipient.
http://www.msa.microscopy.com/MSADocs/MSAAwards.html
* MSA Placement Office
http://www.msa.microscopy.com/PlacementOffice/JobListings.html
* MSA Certification Program
http://www.cvmbs.colostate.edu/emcenter/msa/certboard/
* MSA Technologist Forum
http://www.cvmbs.colostate.edu/emcenter/msa/techforum/
* MSA Public Policy Committee
-remain aware and influence governmental funding opportunities
http://www.msa.microscopy.com/PublicPolicy/
* MSA Sustaining Members Program
http://www.msa.microscopy.com/cgi-bin/SMDBListing.pl
* MSA Vendor News and Surplus Equipment Listings
http://www.msa.microscopy.com/News/NewsListings.html
http://www.msa.microscopy.com/SurplusEquipment/SurplusListings.html
* Reduced prices for books and journals
* Subscription to the MSA Bulletin to keep abreast of Society activities
* Specialized networking via participation in "Focussed Interest Groups" with
microscopist's having similar interests to yours
* and of course the International Networking facilitated in part by
the Microscopy
Listserver to keep abreast of worldwide microscopy activities
and information.

So if your not a member of MSA, take a moment and join!
It is easy and inexpensive [$15/Students, $ 45/Regular,
$63/outside of North America and don't forget this includes the
a subscription to the Journal].

You can become a Member On-Line at
http://www.msa.microscopy.com/MSADocs/MSANewForm.html
or request that an printed application form be sent to you by
sending an Email to:
MSABusinessOffice-at-msa.microscopy.com


Remember MSA needs YOU !
--------------------

MSA Marketing and Communications Committee
MSAMCC-at-msa.microscopy.com


From daemon Mon Jun 24 20:30:03 2002



From: Brendan J. Griffin :      bjg-at-cyllene.uwa.edu.au (by way of
Date: Mon, 24 Jun 2002 20:18:36 -0500
Subject: CMM Biological Microscopy Lectureship - open til 5th July

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear All,

Please note that the closing date for the advertised Biological
Microscopy Lectureship is 5th July, not 28th June.

Thank you
--




Brendan J. Griffin
Director & Assoc. Professor in Electron Microscopy
Centre for Microscopy and Microanalysis
The University of Western Australia
First floor, Physics Building,
35 Stirling Highway
CRAWLEY, WA, AUSTRALIA 6009
ph 61-8-9380-2739 fax 9380-1087
mobile 0409-104-096
bjg-at-cmm.uwa.edu.au
http://cmm.uwa.edu.au/

CRICOS Provider No. 00126G


From daemon Tue Jun 25 10:36:34 2002



From: Deborah Grimm :      dgrimm-at-tulane.edu (by way of MicroscopyListserver)
Date: Tue, 25 Jun 2002 10:16:52 -0500
Subject: TEM Position - Tulane University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position Laboratory Supervisor

Tulane University's Coordinated Instrumentation Facility seeks an individual
to supervise its expanding microscopy facility. The successful candidate
will have a B.S. in a basic science and two years of electron or advanced
microscopy experience. Experience working in a multi-user environment
serving biological and materials science clientele is a plus. Direct
experience with JEOL's SEMs and TEMs as well as confocal microscopy is
desired. The position is responsible for sample preparation, instrument
maintenance and operation.

Deborah A. Grimm, Ph.D.
Director
Coordinated Instrumentation Facility
605 Lindy Boggs Building
Tulane University
New Orleans, LA 70118


From daemon Tue Jun 25 12:07:41 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Tue, 25 Jun 2002 12:40:44 -0400
Subject: Bill Miller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm trying to contact Bill Miller. Could someone send me Bill Millers email
or contact info.

Bill, you sent email to me yesterday about new high res image capture
system. I've lost it. Please contact me again.

Thanks and Regards,


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Tue Jun 25 16:44:17 2002



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Tue, 25 Jun 2002 14:35:52 -0700
Subject: Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Post-Doctoral Associate

Available with the Center for High Resolution Electron Microscopy at
Arizona State University. Position is sponsored by major chemical company
and primary focus of the research will be the development and application
of environmental transmission electron microscopy to industrially relevant
catalyst systems. Areas of particular interest include the study of phase
transformations under reaction environments, in situ sintering, mobility
and dynamic microstructural changes. Candidate will have a Ph.D. in
material science, material physics, solid-state chemistry or chemical
engineering, with experience in nano-particle characterization by in situ
transmission electron microscopy. Experience with the techniques of high
resolution imaging, electron energy loss spectroscopy and scanning
transmission electron microscopy is preferred. Please submit your resume
together and the names and addresses of 3 referees to: Dr. Peter A.
Crozier, Industrial Associates Program, Center for Solid State Science,
Arizona State University, Tempe, AZ 85287-1704, Fax (480) 965-9004, email
crozier-at-asu.edu.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Tue Jun 25 17:24:26 2002



From: Al Coritz :      Cactusgrower-at-earthlink.net
Date: Tue, 25 Jun 2002 18:17:31 -0400
Subject: Bill Miller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill Miller can be reached at microbill-at-mohawk.net

Best,

Al Coritz, Sales Manager
Diatome USA
V: 215-646-1478
F: 215-646-8931
Cactusgrower-at-earthlink.net
www.emsdiasum.com


-----Original Message-----
} From: Goodhouse, Joseph [mailto:jgoodhouse-at-molbio.princeton.edu]
Sent: Tuesday, June 25, 2002 12:41 PM
To: Microscopy (E-mail)


I'm trying to contact Bill Miller. Could someone send me Bill Millers
email
or contact info.

Bill, you sent email to me yesterday about new high res image capture
system. I've lost it. Please contact me again.

Thanks and Regards,


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432

Visit us at
http://www.molbio.princeton.edu/facility/confocal/index.html




From daemon Wed Jun 26 04:16:02 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Wed, 26 Jun 2002 11:03:52 +0200
Subject: Revisiting LaB6

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Technai 12 which we want to convert to LaB6. Since different
microscopes have their own petty preferences (not including the
manufactures). Therefore I would like to know what other manufacturers LaB6
run nicely on a Technai 12? I know Den co was used on the CM range.

It will be a pretty expensive exercise to buy a LaB6 and find it not willing
to marry my Technai!

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana
{ {...OLE_Obj...} }

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}




From daemon Wed Jun 26 06:43:13 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Wed, 26 Jun 2002 13:31:43 +0200
Subject: LaB6 revisited

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

We have a Technai 12 which we want to upgrade with a LaB6. What we would
like to know which other brands of LaB6 marry nicely with the Technai 12. I
am interested in stability and actual life.
Thanks

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana
{ {...OLE_Obj...} }

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}




From daemon Wed Jun 26 07:57:48 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Wed, 26 Jun 2002 08:51:09 -0400
Subject: Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Experienced (20+years) electron microscopist looking for a weekend opening in
a clinical/research lab in the Philadelphia area. f anyone knows of any
opening please feel free to email me.
John Hoffpauir


From daemon Wed Jun 26 08:46:33 2002



From: Lena Falk :      lklfalk-at-fy.chalmers.se
Date: Wed, 26 Jun 2002 15:42:16 +0200
Subject: SEM course at Chalmers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear fellow microscopist,

Take the chance to learn about the developments in scanning electron
microscopy! A course on "Scanning Electron Microscopy - Imaging and
Microanalysis", SEM 2002, will be given at Chalmers University of
Technology, Göteborg, Sweden, October 22 - 24, 2002.

The course will discuss imaging and microanalysis in high vacuum, low
vacuum and environmental SEM’s with special focus on:
o low voltage SEM
o new detectors and detector technology
o electron sources
o elemental and phase analysis
o optimum specimen preparation

The course will be given in close collaboration with microscope
manufacturers and suppliers of equipment for microanalysis.
Instrumentation from the following companies will be used in the course:
SEM’s from Fei, Hitachi, Jeol and Leo, EDX/WDX/EBSP systems from EDAX,
Oxford Instruments and HKL Technology, and equipment for specimen
preparation from Gatan.

This three-day intensive course will give a theoretical background in
the morning sessions and experimental insights in the laboratory
afternoon sessions. The lectures and the demonstrations will be given
by application specialists from the different companies representad at
the course, and also by researchers working with different applications
in SEM. The demonstrations and lab classes will be carried out on
equipment brought to Chalmers specifically for this course.

Detailed information about SEM 2002, including course programme and
registration form, is posted on: http://fy.chalmers.se/microscopy This
web-site will be continuously updated until the course starts. The
registration fee is 6.500 SEK plus VAT, including lecture notes,
refreshments, lunches and course dinner.

Looking forward to seeing you at SEM 2002!

Lena Falk and Mats Halvarsson
Course Organisers

____________________________

Associate Professor Lena Falk
Department of Experimental Physics,
Chalmers University of Technology,
SE-412 96 Göteborg, SWEDEN

tel: +46 31 772 3321
fax: +46 31 772 3224
e-mail: lklfalk-at-fy.chalmers.se


From daemon Wed Jun 26 11:03:34 2002



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Wed, 26 Jun 2002 12:41:10 -0700
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Please note that the entry for the Expert's Session: Core facility managment is incorrect in the program. Although it is listed for 3:00 Wed afternoon, the correct time is 8:00 Wed morning.
The program should have read as follows:

Maintaining Major Equipment in a Core Microscopy Facility

8:15 Introduction

8:30: Servicing by Original Equipment manufacturers: the structure, function & considerations for assembling & operating a major service organization
JEOL: Patrick McGinley
National Service Manager
FEI: Mike Kearney
Director of Service
Hitachi: Greg Rigby
Director of Service

9:15: Servicing EMs by On-site staff: Insight into the Do-it-yourself Approach.
John Wheatley, Arizona State University
Owen Mills, Michigan Technological University

10:00: Break

10:30: Using 3rd Party Service Organizations for Major Equipment Maintenance
Art McCanna
Service Manager
Materials Analytical Services

11:00: Policies Regarding Allowable Costs for Equipment Maintenance on National Science Foundation (NSF) Awards


--------------------------------------





Harry Ekstrom
Materials Laboratory

*Phone: (602) 231-2744
?Fax: (602) 231-1547
*e-mail: harry.ekstrom-at-honeywell.com
{mailto:harry.ekstrom-at-honeywell.com}




From daemon Thu Jun 27 08:08:56 2002



From: David_Bell-at-Millipore.com (by way of MicroscopyListserver)
Date: Thu, 27 Jun 2002 07:54:28 -0500
Subject: Microtomy of Polymers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

I am looking for a service lab in the north east that has the capability
of embedding and sectioning various types of polymer membranes. Please
contact me off list if you can provide such a service.

Thanks in advance,



David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108


From daemon Thu Jun 27 08:08:57 2002



From: reinhard rachel :      reinhard.rachel-at-biologie.uni-regensburg.de (by
Date: Thu, 27 Jun 2002 07:54:03 -0500
Subject: Re: Revisiting LaB6

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are operating a CM12 since about 11 years now,
exclusively with LaB6 filaments (in a Microbiology
environment, no EDX, no STEM, but with Cryo).
I strongly recommend to use this filament type.
If used properly, one filament can last for as long as 3 years;
in our CM12, it is only the 4th filament now.
EM is in use daily, approx. 6 - 10 hrs. Mo - Fr.
3 years of NOT changing a filament, not opening the column:
excellent vacuum! no down times -- if you sum it up,
it even might be cheaper than W-filaments.

I started with Denca; now I'm using the Kimball Physics
filament; a bit more expensive, but recommendation from
the service engineer. Runs very smoothly, no problems.
very satisfied.

if you need more info, contact me directly.

sincerely, yours,

Reinhard Rachel


From daemon Thu Jun 27 12:03:38 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Thu, 27 Jun 2002 12:50:33 -0400
Subject: LaB6 life expectancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

In one of the massages I've read that LaB6 filament lasts for ~3 years. We
run our SEM 16-20 hr per day(heat 5.0-5.3) and turn down heat to 4 for the
night. We only turn off SEM for weekends. My question is how long does LaB6
filament lasts?

Thanks,
Pavel




From daemon Thu Jun 27 12:08:56 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Thu, 27 Jun 2002 13:04:15 -0400
Subject: LaB6 life expectancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

In one of the massages I've read that LaB6 filament lasts for ~3 years. We
run our SEM 16-20 hr per day(heat 5.0-5.3) and turn down heat to 4 for the
night. We only turn off SEM for weekends. My question is how long does LaB6
filament lasts?

Thanks,
Pavel





From daemon Thu Jun 27 15:55:58 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Thu, 27 Jun 2002 16:48:23 -0400
Subject: Re: LaB6 life expectancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the info.
We keep vacuum system ON at all the time, just the electron optics console
we turn OFF before weekends.
This filament is already running for almost 11 months, and I wondered how
much longer would it last. Previously, before I start working here, LaB6 was
changed in ~ 6 months period.
Thanks again for the info.

Pavel



From daemon Thu Jun 27 16:08:41 2002



From: kwalters :      kwalters-at-blue.weeg.uiowa.edu
Date: Thu, 27 Jun 2002 16:02:21 -0500
Subject: Lynx el Tissue Processor for EM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Has anyone had experience with this automated processor of EM samples? Thanks
for any information you can provide me.

Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------



From daemon Thu Jun 27 16:58:44 2002



From: Judy Trogadis :      TrogadisJ-at-smh.toronto.on.ca
Date: Thu, 27 Jun 2002 17:06:55 -0400
Subject: facility management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This topic may have been discussed, if so, I will look at the archives if you tell me an approximate date.

Some of the microscopes in our facility are sometimes left in a sorry state by inexperienced or hasty users. I would like to keep track of users who are actually capturing images, therefore using the computer (if fingerprint sensors exist, let me know, I can just dust the microscope knobs).

Has anyone used software that keeps a record of login ID's and hours of use? We are on NT operating systems.

Thank you

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Queen
30 Bond St.
Toronto, ON
M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Fri Jun 28 06:33:35 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Fri, 28 Jun 2002 07:25:06 -0400
Subject: Re: LaB6 life expectancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Reading you question ,Ritchie, I thought you might be right. The
electronics probably would last longer if I don't turn on and off the
console 50 times a year, but I think TV monitors, would burn out.

Regards,
Pavel



From daemon Fri Jun 28 08:15:07 2002



From: danthony38-at-cogeco.ca (by way of Ask-A-Microscopist)
Date: Fri, 28 Jun 2002 08:04:38 -0500
Subject: Ask-A-Microscopist: Polarizer and Crystals in LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (danthony38-at-cogeco.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
June 26, 2002 at 13:32:34
---------------------------------------------------------------------------

Email: danthony38-at-cogeco.ca
Name: don anthony

Education: 9-12th Grade High School

Location: peterborough ont canada

Question: I have seen beautiful photos of, (as I was told)chemicals
crystalized on glass slides. As it was explained to me, a polorizer
was placed in front of the light source,and a second polorizer, above
the crystals being photographed. The profusion of colour was
unbelieveable, and when the polorizer was moved slightly, the colours
changed, creating a different picture. My question is,after trying to
duplicate this proceedure, I'm getting no where, can anyone help?
Also what chemicals make for the best crystals. Many thanks Don

---------------------------------------------------------------------------


From daemon Fri Jun 28 08:15:07 2002



From: jolanta-at-ksu.edu ()
Date: Fri, 28 Jun 2002 08:03:47 -0500
Subject: Ask-A-Microscopist: stain on pollen- chloral hydrate?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jolanta-at-ksu.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on Monday,
June 24, 2002 at 17:37:44
---------------------------------------------------------------------------

Email: jolanta-at-ksu.edu
Name: Jolanta Jacobs

Organization: KSU

Education: Graduate College

Location: Manhattan, KS USA

Question: Hello,
I need to do a vital stain on pollen of Arabidopsis mutant I'm working on- it
seems Alexander's stain is still widely used, at least outside US. One of
the ingredients is chloral hydrate which happens to be a schedule IV
substance. I called DEA to get the license but it may take longer than I
have the time for. Is there a substance that may be used instead of chloral
hydrate? Or some other as easy as Alexander's vital staining method for
pollen?
Thank you for any input you may have,
Jolanta Jacobs
Grad student in Div of Biology
Manhattan, Kansas


---------------------------------------------------------------------------


From daemon Fri Jun 28 08:26:26 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 28 Jun 2002 09:19:21 -0400
Subject: RE: facility management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Judy,
You need to speak to your network manager. S/he can set up an audit
of resources that will keep track of the information. Also, you should
discuss means by which users may be isolated to specific resources on the
network or local client such that their use of resources can be structured.
User Manager for Domains permits domain-wide auditing of user activities
along with auditing of specific resource use. You will NOT be able to keep
a microscope clean when the user doesn't pay, however. If entry to the
facility/office is by pass card, then there will be a record of entry and
times. Perhaps you might consider placing a sign on the scope that
requests that any user report any scope in poor condition so that you know,
at least, that s/he wasn't the culprit. If you know the previous user, you
can gently chide. For a student, the grade's the thing.
Such things are easier the more widespread is use of NT Wks clients
in your system. If Win 9x is the majority client OS, then you will have to
rely more on domain auditing at the server. NT Wks permits local auditing.
What is called "shell scripting" is the method of choice to specify and
amplify auditing requirements, especially for specific resources (e.g..,
shares, applications, and other objects such as printers). Such 'scripts'
are little more than advanced batch files and may be mounted on NT Wrks
clients to reduce network overhead. Your domain admins should be able to
tailor auditing to your needs. If YOU are the Admin, then you should get
some help from the System Admin.

There is a book by Minasi, Mark, "Mastering Windows NT Server 4",
Sybex, which should be found in the libraries of at least one of your IT
folks. You might also ask about another that I use. Hill, T, "Windows NT
Scripting", Macmillan.

Regards,

Fred Monson

} ----------
} From: Judy Trogadis
} Sent: Thursday, June 27, 2002 5:06 PM
} To: Microscopy-at-sparc5.microscopy.com
} Cc: Judy Trogadis
} Subject: facility management
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This topic may have been discussed, if so, I will look at the archives if
} you tell me an approximate date.
}
} Some of the microscopes in our facility are sometimes left in a sorry
} state by inexperienced or hasty users. I would like to keep track of users
} who are actually capturing images, therefore using the computer (if
} fingerprint sensors exist, let me know, I can just dust the microscope
} knobs).
}
} Has anyone used software that keeps a record of login ID's and hours of
} use? We are on NT operating systems.
}
} Thank you
}
} Judy Trogadis
} Bio-Imaging Coordinator
} St. Michael's Hospital, 8Queen
} 30 Bond St.
} Toronto, ON
} M5B 1W8
} Canada
}
} ph: 416-864-6060 x6337
} fax: 416-864-6043
} trogadisj-at-smh.toronto.on.ca
}
}
}
}


From daemon Fri Jun 28 08:55:04 2002



From: Corazon D. Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Fri, 28 Jun 2002 08:36:42 -0400
Subject: travel to Quebec City

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate clarification on how to get to Quebec city from
Montreal-Dorval airport. The website is not very clear on how to get to
Quebec City i.e. the shuttle service lists the airport to downtown Montreal
but I was not able to locate the shuttle to Quebec City. Or does this mean
to take the shuttle to downtown Montreal and take another shuttle to Quebec
city? Please help.

Thanks,

Cora Bucana
Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747


From daemon Fri Jun 28 10:41:08 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 28 Jun 2002 11:32:15 -0400
Subject: RE: facility management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Judy,
You need to speak to your network manager. S/he can set up an audit
of resources that will keep track of the information. Also, you should
discuss means by which users may be isolated to specific resources on the
network or local client such that their use of resources can be structured.
User Manager for Domains permits domain-wide auditing of user activities
along with auditing of specific resource use. You will NOT be able to keep
a microscope clean when the user doesn't pay, however. If entry to the
facility/office is by pass card, then there will be a record of entry and
times. Perhaps you might consider placing a sign on the scope that
requests that any user report any scope in poor condition so that you know,
at least, that s/he wasn't the culprit. If you know the previous user, you
can gently chide. For a student, the grade's the thing.
Such things are easier the more widespread is use of NT Wks clients
in your system. If Win 9x is the majority client OS, then you will have to
rely more on domain auditing at the server. NT Wks permits local auditing.
What is called "shell scripting" is the method of choice to specify and
amplify auditing requirements, especially for specific resources (e.g..,
shares, applications, and other objects such as printers). Such 'scripts'
are little more than advanced batch files and may be mounted on NT Wrks
clients to reduce network overhead. Your domain admins should be able to
tailor auditing to your needs. If YOU are the Admin, then you should get
some help from the System Admin.

There is a book by Minasi, Mark, "Mastering Windows NT Server 4",
Sybex, which should be found in the libraries of at least one of your IT
folks. You might also ask about another that I use. Hill, T, "Windows NT
Scripting", Macmillan.

Regards,

Fred

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/



} ----------
} From: Judy Trogadis
} Sent: Thursday, June 27, 2002 5:06 PM
} To: Microscopy-at-sparc5.microscopy.com
} Cc: Judy Trogadis
} Subject: facility management
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This topic may have been discussed, if so, I will look at the archives if
} you tell me an approximate date.
}
} Some of the microscopes in our facility are sometimes left in a sorry
} state by inexperienced or hasty users. I would like to keep track of users
} who are actually capturing images, therefore using the computer (if
} fingerprint sensors exist, let me know, I can just dust the microscope
} knobs).
}
} Has anyone used software that keeps a record of login ID's and hours of
} use? We are on NT operating systems.
}
} Thank you
}
} Judy Trogadis
} Bio-Imaging Coordinator
} St. Michael's Hospital, 8Queen
} 30 Bond St.
} Toronto, ON
} M5B 1W8
} Canada
}
} ph: 416-864-6060 x6337
} fax: 416-864-6043
} trogadisj-at-smh.toronto.on.ca
}
}
}
}


From daemon Fri Jun 28 10:58:53 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Fri, 28 Jun 2002 11:52:07 -0400
Subject: Re: facility management (NT user auditing)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Judy,

I believe you can obtain the information you need without installing any additional software. Windows NT has built-in tools for tracking users' logons/logoffs (among other things), though it's not enabled by default.

Click Start -} Programs -} Administrative Tools (Common) -} User Manager
Select the Policies menu -} Audit..., and you'll find a window that allows you to enable auditing, and select which events are audited and logged. In your case, I'd recommend just enabling auditing for successful logons and logoffs. Everything selected will be logged to the Security Event Log: C:\WINNT\system32\config\SecEvent.Evt (Assuming %SystemRoot% is C:\WINNT\).

In order to view, filter, sort, and archive the log, as well as change settings like maximum file size and overwrite policy, use the Event Viewer:
Click Start -} Programs -} Administrative Tools (Common) -} Event Viewer

Use Log -} Security to select the Security Log for viewing. I think you'll find the View -} Filter Events... selection will allow you to do everything you need, without having to import to a spreadsheet.

Best Regards,

Kevin Frischmann

Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: 212-313-7975
Fax: 212-496-3480
email: kfrisch-at-amnh.org


At 05:06 PM 6/27/02 -0400, Judy Trogadis wrote:
} This topic may have been discussed, if so, I will look at the archives if you tell me an approximate date.
}
} Some of the microscopes in our facility are sometimes left in a sorry state by inexperienced or hasty users. I would like to keep track of users who are actually capturing images, therefore using the computer (if fingerprint sensors exist, let me know, I can just dust the microscope knobs).
}
} Has anyone used software that keeps a record of login ID's and hours of use? We are on NT operating systems.
}
} Thank you
}
} Judy Trogadis
} Bio-Imaging Coordinator
} St. Michael's Hospital, 8Queen
} 30 Bond St.
} Toronto, ON
} M5B 1W8
} Canada
}
} ph: 416-864-6060 x6337
} fax: 416-864-6043
} trogadisj-at-smh.toronto.on.ca



From daemon Fri Jun 28 11:12:21 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Fri, 28 Jun 2002 12:05:28 -0400
Subject: Re: facility management (NT user auditing)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Judy,

I believe you can obtain the information you need without installing any additional software. Windows NT has built-in tools for tracking users' logons/logoffs (among other things), though it's not enabled by default.

Click Start -} Programs -} Administrative Tools (Common) -} User Manager
Select the Policies menu -} Audit..., and you'll find a window that allows you to enable auditing, and select which events are audited and logged. In your case, I'd recommend just enabling auditing for successful logons and logoffs. Everything selected will be logged to the Security Event Log: C:\WINNT\system32\config\SecEvent.Evt (Assuming %SystemRoot% is C:\WINNT\).

In order to view, filter, sort, and archive the log, as well as change settings like maximum file size and overwrite policy, use the Event Viewer:
Click Start -} Programs -} Administrative Tools (Common) -} Event Viewer

Use Log -} Security to select the Security Log for viewing. I think you'll find the View -} Filter Events... selection will allow you to do everything you need, without having to import to a spreadsheet.

Best Regards,

Kevin Frischmann

Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: 212-313-7975
Fax: 212-496-3480
email: kfrisch-at-amnh.org


} At 05:06 PM 6/27/02 -0400, Judy Trogadis wrote:
} This topic may have been discussed, if so, I will look at the archives if you tell me an approximate date.
}
} Some of the microscopes in our facility are sometimes left in a sorry state by inexperienced or hasty users. I would like to keep track of users who are actually capturing images, therefore using the computer (if fingerprint sensors exist, let me know, I can just dust the microscope knobs).
}
} Has anyone used software that keeps a record of login ID's and hours of use? We are on NT operating systems.
}
} Thank you
}
} Judy Trogadis
} Bio-Imaging Coordinator
} St. Michael's Hospital, 8Queen
} 30 Bond St.
} Toronto, ON
} M5B 1W8
} Canada
}
} ph: 416-864-6060 x6337
} fax: 416-864-6043
} trogadisj-at-smh.toronto.on.ca



From daemon Fri Jun 28 11:53:46 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Fri, 28 Jun 2002 12:45:13 -0400 (EDT)
Subject: Re: facility management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Judy,

I believe you can obtain the information you need without installing any
additional software. Windows NT has built-in tools for tracking users'
logons/logoffs (among other things), though it's not enabled by default.

Click Start -} Programs -} Administrative Tools (Common) -} User Manager
Select the Policies menu -} Audit..., and you'll find a window that allows
you to enable auditing, and select which events are audited and logged.
In your case, I'd recommend just enabling auditing for successful logons
and logoffs. Everything selected will be logged to the Security Event
Log: C:\WINNT\system32\config\SecEvent.Evt (Assuming %SystemRoot% is
C:\WINNT\).

In order to view, filter, sort, and archive the log, as well as change
settings like maximum file size and overwrite policy, use the Event
Viewer:
Click Start -} Programs -} Administrative Tools (Common) -} Event Viewer

Use Log -} Security to select the Security Log for viewing. I think
you'll find the View -} Filter Events... selection will allow you to do
everything you need, without having to import to a spreadsheet.

This is the easiest to configure option I can think of; If you need
something a bit more sophisticated, and/or need to audit a large number of
workstations, Fred Monson's suggestions are certainly more appropriate.

Best Regards,

Kevin Frischmann

Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: 212-313-7975
Fax: 212-496-3480
email: kfrisch-at-amnh.org




At 05:06 PM 6/27/02 -0400, Judy Trogadis wrote:
This topic may have been discussed, if so, I will look at the archives if
you tell me an approximate date.

Some of the microscopes in our facility are sometimes left in a sorry
state by inexperienced or hasty users. I would like to keep track of users
who are actually capturing images, therefore using the computer (if
fingerprint sensors exist, let me know, I can just dust the microscope
knobs).

Has anyone used software that keeps a record of login ID's and hours of
use? We are on NT operating systems.

Thank you

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Queen
30 Bond St.
Toronto, ON
M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Fri Jun 28 20:54:15 2002



From: rcmoretz-at-att.net
Date: Sat, 29 Jun 2002 01:41:12 +0000
Subject: Re: Lynx el Tissue Processor for EM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kathy:
I have had experience with the Lynx processor for almost
as long as they have been on the market--first directly,
then thru Leica. For routine processing of tissues, the
Lynx offers the opportunity to do a one time setup for
processing from fixative to epoxy:dehydrant mix. I
currently have programs for OsO4 thru pure epoxy,
including uranyl acetate en bloc staining that runs for
more than 24 hours. The only caveat I have for the pure
epoxy (epon replacement) is that I run overnight up to
pure, mix and add the pure the following morning (I am
reluctant to let the complete epoxy mix stand overnight--
there's too much polymerization going on, even at RT).
The programming is simple and will allow up to 9
separate regimens (I have only 4 plus a test routine set
up), and alteration is simple to allow for different
start time, etc. The unit has a Peltier cooled chamber
that will permit 4C up to RT or higher for the different
steps. There is an agitation feature, etc. The maximum
number of samples (separate wells) is 28, altho'
chambers are available for 9 wells. It is possible to
us the Lynx for TEM grid staining--but I have to admit
that I don't trust the system for doing lead staining!
So, I've never done that.
All is not always great. I had no trouble with the
original unit, which is why I purchased the second one
(13 years ago). But it was the proverbial lemon. It
took nearly 5 years to get it functional and it
generally operates flawlessly--altho' I still have
gremlin-like problems now and again to remind me that it
really ought to be a bright yellow color. I now
purchase supplies thru' EMS and use the unit for
processing large volumes of tissues for critical
studies. Proper attention to details, careful cleaning
and maintenance and not over-reusing the reagent vials
will aid in improved,consistent operational
performance. (Indeed, the last problem I had was
operator-induced, from using a warped, reused reagent
vial!) Like any other equipment, the Lynx is a
potentially great time and effort saver, but poor
routines (and the occasional lemon) can result in severe
headaches (the thing _always_ goes off at about 2am!).

Roger
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} Has anyone had experience with this automated processor of EM samples? Thanks
} for any information you can provide me.
}
} Kathy
}
} Kathy Walters //
} Central Microscopy Research Facility / /
} 85 EMRB / /\
} University of Iowa / /\ \
} Iowa City, Iowa 52242 / / \ \
} Phone #: (319) 335-8142 / / \ \
} Fax #: (319) 384-4469 ______ ((0))
} email: Katherine-Walters-at-uiowa.edu |__| / /
} || / /
} --------------
} ------------------
}
}


From daemon Sat Jun 29 10:35:51 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 29 Jun 2002 08:14:24 -0700
Subject: Re: Ask-A-Microscopist: Polarizer and Crystals in LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

We need to know more about what you've tried. What polarizers are you
using? When you look thru the pair & rotate one, does the field go really
dark, or just a bit more grey? What did you crystallize? Is it a thin
layer? These websites might help:
http://www.crystal-land.com , http://www.pbrc.hawaii.edu/~kunkel/,
http://www.cellsalive.com, http://home.att.net/~seberhard,
http://www.ScienceArt.nl

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon Jul 1 05:00:07 2002



From: Arthur Day :      ard-at-ansto.gov.au
Date: Mon, 1 Jul 2002 19:39:31 +1000
Subject: Turning machines off: (was LaB6 life expectancy)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Pavel,

I hope this doesn't start off a thorny side debate about the pros and
cons of turning our microscopes off or not when not in use, but I
think that Ritchie is correct. I don't know your local situation but
unless there is some really compelling reason to turn the electron
optics console off on weekends then you should ideally leave it on
permanently as you do the vacuum system. (Just blank out or turn down
the brightness if you are worried about "burn marks" from characters
on the TV monitor screens?) There are at least two elements to this:

1. Even if leaving the electronics on all of the time means that you
have to replace the monitor screens every few years, the risk of
other much more serious (read expensive) things going wrong over the
microscope's service life as a result of cycling the power hundreds
of times, is probably a lot more significant. The replacement costs
for the monitor screens would generally be peanuts compared with the
costs of diagnosing and replacing some of the other dozens of bits
and pieces in the electronics console, especially any one of a large
number of individual boards that could fail. Add to that the cost of
the down time while you wait for replacement parts. Usually you get a
fair bit of warning from an ageing monitor screen via gradual loss of
picture quality, so you can change it at leisure, and replacement
costs should only be in the order of hundreds of dollars or less
rather than the all-up costs in the thousands of dollars or more for
many components in the rest of the machine.

2. In any case there is a risk that turning the electron optics
console on and off hundreds of times could abruptly shorten the life
of the monitor tubes anyway -- even more than simply leaving them on,
because thermal cycling of the filament will place mechanical
stresses on the filament itself, the other gun components, and
metal-glass seals etc.

I suppose the disclaimer is that of course the optimum on/off regime
is dependent on one's particular local situation.

}
} Reading you question ,Ritchie, I thought you might be right. The
} electronics probably would last longer if I don't turn on and off the
} console 50 times a year, but I think TV monitors, would burn out.
}
} Regards,
} Pavel





Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/


From daemon Mon Jul 1 07:29:02 2002



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Tue, 25 Jun 2002 13:21:54 +0100
Subject: recirculating chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I am interested in recirculating chillers that can be attached to
electropolishing units. These 'chillers' pump coolant to the
electropolishing unit and the temperatures range that I require needs to
be as far down as -40°C.
If someone has one could they send me the brand/make?

Thanks in advance,
Steven






From daemon Mon Jul 1 07:34:12 2002



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Tue, 25 Jun 2002 13:29:19 +0100
Subject: recirculating chillers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I am interested in recirculating chillers that can be attached to
electropolishing units. These 'chillers' pump coolant to the
electropolishing unit and the temperatures range that I require needs to
be as far down as -40°C.
If someone has one could they send me the brand/make?

Thanks in advance,
Steven

--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Mon Jul 1 08:37:54 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 1 Jul 2002 09:23:57 -0400
Subject: Re: travel to Quebec City

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cora,

I booked my flight right into Quebec City. Check your arrangements.
Montreal is a long way from Quebec City.


Lee

At 8:36 AM -0400 6/28/02, Corazon D. Bucana wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Jul 1 09:48:53 2002



From: Petr Schauer :      Petr-at-isibrno.cz
Date: Mon, 1 Jul 2002 16:40:28 +0200
Subject: Conference list 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear microscopists,

the initial microscopy conference collection for the year 2003 has
appeared at the Petr's Microscopy Resources at the

http://www.petr.isibrno.cz/microscopy/meetings.php#2003

Petr Schauer
+--------------------------------------------------------------------+
| Dr. Petr Schauer tel.: (+420 5) 41514313 |
| Head of Electron Microscopy Laboratory fax : (+420 5) 41514404 |
| INSTITUTE OF SCIENTIFIC INSTRUMENTS (+420 5) 41514402 |
| ACADEMY OF SCIENCES OF THE CZECH REPUBLIC e-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno petr-at-dp.fce.vutbr.cz |
| Czech Republic www: http://www.petr.isibrno.cz/ |
+--------------------------------------------------------------------+



From daemon Mon Jul 1 09:51:18 2002



From: David.Cugier-at-abbott.com
Date: Mon, 1 Jul 2002 09:45:08 -0500
Subject: Re: Lynx el Tissue Processor for EM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Kathy,

I have been using the older Leica Lynx tissue processor (Now distributed by
Electron Microscopy Sciences under the name Vision EMS processor) for 6 years
and have been extremely pleased with the reliability, efficiency, and quality.
There are a few potential short falls inherent with any automated tissue
processor versus processing by hand which include the incorporation of air
bubbles which can get trapped in the processing baskets and prevent good fluid
exchange with the tissue. Also there is the potential problem of vials
jamming if the vials are not seated securely in the carousel or if the vials
are not symetrically round. I have not experienced jamming vials but I know
this can be a problem if you are not careful. The automated tissue processors
are extremely helpful in our lab because we process thousands of samples a
year and the overall sample preservation is good.


David Cugier
Associate Cellular and Molecular Biologist
Abbott Labs
847-938-6725
David.Cugier-at-Abbott.com



kwalters
{kwalters-at-blue.weeg. To: Microscopy-at-sparc5.microscopy.com
uiowa.edu} cc:
Subject: Lynx el Tissue Processor for EM samples
06/27/02 04:02 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

Has anyone had experience with this automated processor of EM samples? Thanks
for any information you can provide me.

Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------







From daemon Mon Jul 1 10:01:08 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 1 Jul 2002 10:54:38 -0400
Subject: RE: Ask-A-Microscopist: Polarizer and Crystals in LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Don,

Please begin with:

http://micro.magnet.fsu.edu/primer/techniques/polarized/polarizedhome.html

Then, to get your colors from polarized light, you must understand
the phenomenon:

http://micro.magnet.fsu.edu/primer/virtual/polarizing/

The key to having command of any tool is to understand how it works! The
microscope IS a tool!

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: danthony38-at-cogeco.ca
} Sent: Friday, June 28, 2002 9:04 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: Polarizer and Crystals in LM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (danthony38-at-cogeco.ca) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
} June 26, 2002 at 13:32:34
} --------------------------------------------------------------------------
} -
}
} Email: danthony38-at-cogeco.ca
} Name: don anthony
}
} Education: 9-12th Grade High School
}
} Location: peterborough ont canada
}
} Question: I have seen beautiful photos of, (as I was told)chemicals
} crystalized on glass slides. As it was explained to me, a polorizer
} was placed in front of the light source,and a second polorizer, above
} the crystals being photographed. The profusion of colour was
} unbelieveable, and when the polorizer was moved slightly, the colours
} changed, creating a different picture. My question is,after trying to
} duplicate this proceedure, I'm getting no where, can anyone help?
} Also what chemicals make for the best crystals. Many thanks Don
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Mon Jul 1 10:02:43 2002



From: David.Cugier-at-abbott.com
Date: Mon, 1 Jul 2002 09:56:28 -0500
Subject: Re: Lynx el Tissue Processor for EM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Kathy,

I have been using the older Leica Lynx tissue processor (Now distributed by
Electron Microscopy Sciences under the name Vision EMS processor) for 6 years
and have been extremely pleased with the reliability, efficiency, and quality.
There are a few potential short falls inherent with any automated tissue
processor versus processing by hand which include the incorporation of air
bubbles which can get trapped in the processing baskets and prevent good fluid
exchange with the tissue. Also there is the potential problem of vials
jamming if the vials are not seated securely in the carousel or if the vials
are not symetrically round. I have not experienced jamming vials but I know
this can be a problem if you are not careful. The automated tissue processors
are extremely helpful in our lab because we process thousands of samples a
year and the overall sample preservation is good.


David Cugier
Associate Cellular and Molecular Biologist
Abbott Labs
847-938-6725
David.Cugier-at-Abbott.com



kwalters
{kwalters-at-blue.weeg. To: Microscopy-at-sparc5.microscopy.com
uiowa.edu} cc:
Subject: Lynx el Tissue Processor for EM samples
06/27/02 04:02 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

Has anyone had experience with this automated processor of EM samples? Thanks
for any information you can provide me.

Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------







From daemon Mon Jul 1 10:07:41 2002



From: David.Cugier-at-abbott.com
Date: Mon, 1 Jul 2002 10:01:20 -0500
Subject: Re: Lynx el Tissue Processor for EM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Kathy,

I have been using the older Leica Lynx tissue processor (Now distributed by
Electron Microscopy Sciences under the name Vision EMS processor) for 6 years
and have been extremely pleased with the reliability, efficiency, and quality.
There are a few potential short falls inherent with any automated tissue
processor versus processing by hand which include the incorporation of air
bubbles which can get trapped in the processing baskets and prevent good fluid
exchange with the tissue. Also there is the potential problem of vials
jamming if the vials are not seated securely in the carousel or if the vials
are not symetrically round. I have not experienced jamming vials but I know
this can be a problem if you are not careful. The automated tissue processors
are extremely helpful in our lab because we process thousands of samples a
year and the overall sample preservation is good.


David Cugier
Associate Cellular and Molecular Biologist
Abbott Labs
847-938-6725
David.Cugier-at-Abbott.com



kwalters
{kwalters-at-blue.weeg. To: Microscopy-at-sparc5.microscopy.com
uiowa.edu} cc:
Subject: Lynx el Tissue Processor for EM samples
06/27/02 04:02 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

Has anyone had experience with this automated processor of EM samples? Thanks
for any information you can provide me.

Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------







From daemon Mon Jul 1 10:21:11 2002



From: David.Cugier-at-abbott.com
Date: Mon, 1 Jul 2002 10:14:25 -0500
Subject: Re: Lynx el Tissue Processor for EM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Kathy,

I have been using the older Leica Lynx tissue processor (Now distributed by
Electron Microscopy Sciences under the name Vision EMS processor) for 6 years
and have been extremely pleased with the reliability, efficiency, and quality.
There are a few potential short falls inherent with any automated tissue
processor versus processing by hand which include the incorporation of air
bubbles which can get trapped in the processing baskets and prevent good fluid
exchange with the tissue. Also there is the potential problem of vials
jamming if the vials are not seated securely in the carousel or if the vials
are not symetrically round. I have not experienced jamming vials but I know
this can be a problem if you are not careful. The automated tissue processors
are extremely helpful in our lab because we process thousands of samples a
year and the overall sample preservation is good.


David Cugier
Associate Cellular and Molecular Biologist
Abbott Labs
847-938-6725
David.Cugier-at-Abbott.com



kwalters
{kwalters-at-blue.weeg. To: Microscopy-at-sparc5.microscopy.com
uiowa.edu} cc:
Subject: Lynx el Tissue Processor for EM samples
06/27/02 04:02 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

Has anyone had experience with this automated processor of EM samples? Thanks
for any information you can provide me.

Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------







From daemon Mon Jul 1 10:27:18 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 1 Jul 2002 11:20:52 -0400
Subject: FW: Ask-A-Microscopist: Polarizer and Crystals in LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Morning Don,
}
} Please begin with:
}
} http://micro.magnet.fsu.edu/primer/techniques/polarized/polarizedhome.html
}
} Then, to get your colors from polarized light, you must understand
} the phenomenon:
}
} http://micro.magnet.fsu.edu/primer/virtual/polarizing/
}
} The key to having command of any tool is to understand how it works! The
} microscope IS a tool!
}
} Regards,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} West Chester University
} South Church Street and Rosedale
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
} ----------
} From: danthony38-at-cogeco.ca
} Sent: Friday, June 28, 2002 9:04 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: Polarizer and Crystals in LM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (danthony38-at-cogeco.ca) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
} June 26, 2002 at 13:32:34
} --------------------------------------------------------------------------
} -
}
} Email: danthony38-at-cogeco.ca
} Name: don anthony
}
} Education: 9-12th Grade High School
}
} Location: peterborough ont canada
}
} Question: I have seen beautiful photos of, (as I was told)chemicals
} crystalized on glass slides. As it was explained to me, a polorizer
} was placed in front of the light source,and a second polorizer, above
} the crystals being photographed. The profusion of colour was
} unbelieveable, and when the polorizer was moved slightly, the colours
} changed, creating a different picture. My question is,after trying to
} duplicate this proceedure, I'm getting no where, can anyone help?
} Also what chemicals make for the best crystals. Many thanks Don
}
} --------------------------------------------------------------------------
} -
}
}
}


From daemon Mon Jul 1 13:08:03 2002



From: Abdul Rani, Suriani :      suriani-at-erc.montana.edu
Date: Mon, 1 Jul 2002 11:58:44 -0600
Subject: Calibration in MetaMorph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

We have a problem here, whereby the picture taken using the confocal
microscopy is distorted in MetaMorph.The picture taken is 1mm by 1mm, but
when the planes are rotated(90° angle), the x and y axis seem distored. I
have been trying to calibrate the xy distances, but it doesn't seem to work.
I was wondering if anyone can inform me the exact ways of calibrating the
distances, or the reasons for the pictures being distorted.

Thank you for your help.

Suriani Abdul Rani
Control Lab
CBE,MSU
Montana

suriania-at-erc.montana.edu


From daemon Mon Jul 1 17:13:49 2002



From: tartenon-at-netscape.net
Date: Mon, 01 Jul 2002 18:05:20 -0400
Subject: RE: Calibration in MetaMorph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Easiest way to do this is contacting UIC at http://www.universal-imaging.com and follow the links to support, they have on site scientific to help you out.
"Abdul Rani, Suriani" {suriani-at-erc.montana.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi!
}
} We have a problem here, whereby the picture taken using the confocal
} microscopy is distorted in MetaMorph.The picture taken is 1mm by 1mm, but
} when the planes are rotated(90° angle), the x and y axis seem distored. I
} have been trying to calibrate the xy distances, but it doesn't seem to work.
} I was wondering if anyone can inform me the exact ways of calibrating the
} distances, or the reasons for the pictures being distorted.
}
} Thank you for your help.
}
} Suriani Abdul Rani
} Control Lab
} CBE,MSU
} Montana
}
} suriania-at-erc.montana.edu
}
}


__________________________________________________________________
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From daemon Mon Jul 1 18:53:10 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Mon, 1 Jul 2002 08:31:56 -0700
Subject: Re: facility management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ed,

I would have to say that the blame is not so much on NT as it is on typical user practices. The security of any system relies a great deal on the security practices of its users.

That said - there are, not surprisingly, a number of solutions to the problem along the lines of what you suggest. Since system administration is a secondary part of many readers' job descriptions (myself included), I feel emphasis ought to be on easy to configure/implement. (I don't know any details on scripting a command to execute after a specified period without input; perhaps "at")

I should mention first that I don't use auditing or auto-logout, so I can't claim first-hand experience that might allow me to point out possible pitfalls.
I do, however, always set the system to lock after a specified period (see below).

If the system is controlling instrumentation, or runs processes that take a long time (users likely to get coffee/lunch while waiting), I would suggest that the system be set to lock, rather than logoff. An auto-logoff will either hang waiting for applications to terminate, or terminate all applications if properly configured (losing unsaved data). This could be a real problem for some instruments, and upsetting for some users. If the system is set to lock, and a user forgets to logoff, anyone with administrative privileges (presumably you) can unlock the workstation in order to properly logoff the last user.

The easiest way I know to setup a local session timeout is to setup a password-protected screensaver. The first step here is to make sure users can't change the screensaver settings. This is best done using the NT system policy editor (NOT the Win 9x version). This is included with NT Server, and perhaps with the Resource Kit as well - I don't know of other ways to get it, but there may be. It can be installed on an NT workstation, using the NT Server disk.

If you have no way to obtain the policy editor, access to the screensaver tab (as well as just about anything else) can also be restricted by manually editing the registry. After restricting access to the screensaver settings, proceed as below:

To setup a lock timeout, just go to the screensaver tab, set the wait time reasonably short, and fill the "password protected" check-box -- doesn't get any easier!

To setup an auto-logoff timeout, you'll need to install and configure the winexit screensaver (included in the resource kit), as well as edit some permissions to keep it from hanging when trying to shut down programs.

My apologies to all if this has begun to wander a bit from the scope of this list, but many of us have to deal with these issues without proper background or support. (i.e. "winging it")

Ed: if you need further details I would suggest contacting me off the list.

Best,

Kevin Frischmann

Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA


------------------------------------------------------------------------

To: Kevin Frischmann {kfrisch-at-amnh.org}
Cc: Microscopy-at-sparc5.microscopy.com


Judy,

I believe you can obtain the information you need without installing any
additional software. Windows NT has built-in tools for tracking users'
logons/logoffs (among other things), though it's not enabled by default.

Click Start -} Programs -} Administrative Tools (Common) -} User Manager
Select the Policies menu -} Audit..., and you'll find a window that allows
you to enable auditing, and select which events are audited and logged.
In your case, I'd recommend just enabling auditing for successful logons
and logoffs. Everything selected will be logged to the Security Event
Log: C:\WINNT\system32\config\SecEvent.Evt (Assuming %SystemRoot% is
C:\WINNT\).

In order to view, filter, sort, and archive the log, as well as change
settings like maximum file size and overwrite policy, use the Event
Viewer:
Click Start -} Programs -} Administrative Tools (Common) -} Event Viewer

Use Log -} Security to select the Security Log for viewing. I think
you'll find the View -} Filter Events... selection will allow you to do
everything you need, without having to import to a spreadsheet.

This is the easiest to configure option I can think of; If you need
something a bit more sophisticated, and/or need to audit a large number of
workstations, Fred Monson's suggestions are certainly more appropriate.

Best Regards,

Kevin Frischmann

Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: 212-313-7975
Fax: 212-496-3480
email: kfrisch-at-amnh.org




At 05:06 PM 6/27/02 -0400, Judy Trogadis wrote:
This topic may have been discussed, if so, I will look at the archives if
you tell me an approximate date.

Some of the microscopes in our facility are sometimes left in a sorry
state by inexperienced or hasty users. I would like to keep track of users
who are actually capturing images, therefore using the computer (if
fingerprint sensors exist, let me know, I can just dust the microscope
knobs).

Has anyone used software that keeps a record of login ID's and hours of
use? We are on NT operating systems.

Thank you

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Queen
30 Bond St.
Toronto, ON
M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca








From daemon Tue Jul 2 11:02:20 2002



From: kwalters :      kwalters-at-blue.weeg.uiowa.edu
Date: Tue, 2 Jul 2002 10:44:53 -0500
Subject: Leica EM processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The responses from the request for comments about the Lynx EM processor were
extremely helpful, so I thought I'd go out on a limb and see if anyone had
comments about the Leica EM processor.

I have collected the Lynx responses in a file and would be glad to send it on
if anyone needs this information.

Thanks,
Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------



From daemon Tue Jul 2 11:13:57 2002



From: kwalters :      kwalters-at-blue.weeg.uiowa.edu
Date: Tue, 2 Jul 2002 11:07:45 -0500
Subject: Leica EM Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The responses from the request for comments about the Lynx EM processor were
extremely helpful, so I thought I'd go out on a limb and see if anyone had
comments about the Leica EM processor.

I have collected the Lynx responses in a file and would be glad to send it on
if anyone needs this information.

Thanks,
Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------



From daemon Tue Jul 2 15:38:34 2002



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Tue, 02 Jul 2002 16:19:21 -0400
Subject: silver tarnish analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers-

i'm looking at some daguerreotypes (early photographs on Ag coated Cu
plates) in the sem to find out about the silver tarnish that forms on the
surfaces. as expected i find sulphur in it, but not in all the areas that
look tarnished. i suspect that since the AgS layer may be thin when
compared to the beam penetration, and that the underlying material is all
Ag (with some Au toning agent) i may be swamping the signal from the
tarnish layer. curiously the areas with the most intense interference
colors are not the highest in S (no O present either). i've used low HV as
well as high. since this is on museum materials i can't prep a cross
section to see what's going on.

so the questions: will a tarnish layer on silver form a wedge of varying
thickness around an aperture to the ambient air? and if a wedge forms will
the interference colors indicate the local thicknesses accurately? does
light play any role in tarnishing (some areas beneath a matte paper look
untarnished, while adjacent areas outside the matte look heavily
tarnished)? and as for the vanishing S signal...any ideas? is there a
better way to probe the tarnish for thickness and composition (like SIMS)?

thx in advance.
b-


----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875


From daemon Wed Jul 3 08:58:09 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 03 Jul 2002 08:45:59 -0500
Subject: copper braid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
I am trying to find a source for heavy copper braid wire to repair some accessories used for metal and carbon evaporation. This is the stiff but flexible wire that would go between the connectors in a high vacuum evaporator and, in this case, the carbon evaporation apparatus. Does anyone have a source in the USA who is likely to sell small quantities? I just need a couple of feet at most.

Thanks,
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907




From daemon Wed Jul 3 09:21:27 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 3 Jul 2002 06:48:11 -0700
Subject: Nikon Coolscan 8000: EM film holder modification

Contents Retrieved from Microscopy Listserver Archives
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


} From: Majid Ghoddusi (mghoddusi-at-cmri.usyd.edu.au)

Hi all,

We are considering to purchase a Nikon SuperCoolscan 8000. Our TEM negative
size is 8.0x9.9 cm and from reading of previous postings on this subject it
appears that the film holder needs a bit of modification for TEM negatives
to fit in without going through the hassle of trimming them (I am aware that
the maximum area that I can scan is about 6.0x9.0, I just want to avoid
trimming down hundreds of negatives).

I would really appreciate it if colleagues who have tried it (modifying the
holder) successfully could please share their experience with me. I have
only seen the 120/220 film holder and it did not seem to be very easy to
modify.

Regards
majid

.....................................................
Majid Ghoddusi, DVM PhD
Senior Microscopist
Muscle Development Unit
Children's Medical Research Institute
locked Bag 23
Wentworthville NSW 2145
Australia
http://www.cmri.com.au
........................................................




From daemon Wed Jul 3 09:30:37 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 3 Jul 2002 07:22:32 -0700
Subject: EM film holder modification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: Majid Ghoddusi (mghoddusi-at-cmri.usyd.edu.au)

Hi all,

We are considering to purchase a Nikon SuperCoolscan 8000. Our TEM negative
size is 8.0x9.9 cm and from reading of previous postings on this subject it
appears that the film holder needs a bit of modification for TEM negatives
to fit in without going through the hassle of trimming them (I am aware that
the maximum area that I can scan is about 6.0x9.0, I just want to avoid
trimming down hundreds of negatives).

I would really appreciate it if colleagues who have tried it (modifying the
holder) successfully could please share their experience with me. I have
only seen the 120/220 film holder and it did not seem to be very easy to
modify.

Regards
majid

....................................................
Majid Ghoddusi, DVM PhD
Senior Microscopist
Muscle Development Unit
Children's Medical Research Institute
locked Bag 23
Wentworthville NSW 2145
Australia
http://www.cmri.com.au
.......................................................




From daemon Wed Jul 3 12:29:36 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 03 Jul 2002 13:23:49 -0400
Subject: Re: copper braid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debby,

Try any electronics store, this kind of material is commonly used for
attaching earth grounds, buss bars and the like. It also is used as a
solder removal aid, or wick. (In the latter case, it is held against a
connection that is to be de-soldered and heated with a soldering iron.
The solder melts and wicks its way into the briad toward the heat,
leaving the joint exposed.)

Too thin for your use? Braid three strands together, etc.

John Twilley

Debby Sherman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers,
} I am trying to find a source for heavy copper braid wire to repair some accessories used for metal and carbon evaporation. This is the stiff but flexible wire that would go between the connectors in a high vacuum evaporator and, in this case, the carbon evaporation apparatus. Does anyone have a source in the USA who is likely to sell small quantities? I just need a couple of feet at most.
}
} Thanks,
} Debby
}
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907
}
}
}
}
}



From daemon Wed Jul 3 13:46:06 2002



From: John Foust :      jfoust-at-threedee.com
Date: Wed, 03 Jul 2002 13:35:11 -0500
Subject: Re: copper braid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 08:45 AM 7/3/2002 -0500, Debby Sherman wrote:
} I am trying to find a source for heavy copper braid wire to repair some accessories used for metal and carbon evaporation. This is the stiff but flexible wire that would go between the connectors in a high vacuum evaporator and, in this case, the carbon evaporation apparatus. Does anyone have a source in the USA who is likely to sell small quantities? I just need a couple of feet at most.

Unless you have special requirements for purity, just go to a
well-equipped hardware store and ask for "copper grounding wire",
a heavy gauge (6?) braid.

- John



From daemon Wed Jul 3 15:18:54 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 03 Jul 2002 16:18:24 -0400
Subject: Re: silver tarnish analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian,

If you haven't already done so, I would suggest that you consult the
people in the Image Permanence Institute of your own university. There
are individuals there, or formerly associated with it, who have done
considerable work in this area. Irving Pobboravsky comes to mind as one
of the few contemporary practitioners who might be able to provide
examples that could be sacrificed.

Just what the image forming structures are in a daguerreotype, and what
interferes with their optical effects, continue to be a subject of
research. The physical state of the surface has much to do with the
scattering of light, and tarnish effects lead to short range
redistribution of material in addition to the expected chemical
reactions. One might expect to find some silver amalgam present as well.

SIMS certainly would seem to be a valuable option. The material would
easily lend itself to ESCA or Auger techniques, as well.

John Twilley
Conservation Scientist

Brian McIntyre wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi listers-
}
} i'm looking at some daguerreotypes (early photographs on Ag coated Cu
} plates) in the sem to find out about the silver tarnish that forms on the
} surfaces. as expected i find sulphur in it, but not in all the areas that
} look tarnished. i suspect that since the AgS layer may be thin when
} compared to the beam penetration, and that the underlying material is all
} Ag (with some Au toning agent) i may be swamping the signal from the
} tarnish layer. curiously the areas with the most intense interference
} colors are not the highest in S (no O present either). i've used low HV as
} well as high. since this is on museum materials i can't prep a cross
} section to see what's going on.
}
} so the questions: will a tarnish layer on silver form a wedge of varying
} thickness around an aperture to the ambient air? and if a wedge forms will
} the interference colors indicate the local thicknesses accurately? does
} light play any role in tarnishing (some areas beneath a matte paper look
} untarnished, while adjacent areas outside the matte look heavily
} tarnished)? and as for the vanishing S signal...any ideas? is there a
} better way to probe the tarnish for thickness and composition (like SIMS)?
}
} thx in advance.
} b-
}
}
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875
}
}
}



From daemon Thu Jul 4 06:52:57 2002



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Thu, 04 Jul 2002 21:41:30 +1000
Subject: Postdoc in multidisiplinary field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A significant component of this work could involve microscopy

Dear colleague,

A postdoctoral fellowship is available at the Centre for Visual
Sciences, Australian National University. It would be appreciated if you
could bring the attached advertisement to the attention of potential
candidates.

yours sincerely

Gert Stange

ANUTECH Pty Ltd

and

AUSTRALIAN NATIONAL UNIVERSITY

Postdoctoral Fellow in Neurophysiology/Biorobotics

The Centre for Visual Sciences is seeking to fill a position to work on a
challenging research project investigating the principles of visual flight
control in insects. The research project, funded by a major aerospace
organization, aims at identifying the spatial transfer characteristics of
the optics and the spatiotemporal characteristics of the neuronal circuitry
associated with the simple eyes (ocelli) of dragonflies. The successful
applicant will apply optical, neuroanatomical, electrophysiological and
behavioural techniques, with specific attention to object/motion detection
mechanisms in the ocellar system and their integration with neuronal flight
control circuitry.
The biological results will be applied to the development of concepts for
novel attitude control systems capable of being implemented in ultra-light
hardware for application to micro-unmanned aerial vehicles.

Salary range will be $A 45,000 to $A 55,000. The position is for 1 year
initially, with prospects for extension for a further 2 years subject to
satisfactory performance and availability of project funding. Enquiries
should be directed to Dr. Gert Stange, ph +61 2 6125 5089, email
gert.stange-at-anu.edu.au. The selection criteria and duty statement can be
obtained from or from Beverley Cooper, ph +61 2 6125 5865, email .

Applications close on Friday 17 August, 2002.

Applications, including the names and contact details of 3 referees, should
be sent to Ms. Beverley Cooper, ANUTECH Pty Ltd., GPO Box 4, Canberra ACT
2601, fax +61 2 6257 1433.




From daemon Thu Jul 4 09:29:36 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 04 Jul 2002 09:20:25 -0500
Subject: copper braid

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Thanks for all the replies regarding sources for copper braid. Most of the braid is tinned on the outside and can be either flat or round. A few of the responses are below.

1) If you disassemble co-axial cable you will find some copper braid wire within. Just remove this, attach crimp-on ends and you ae all set.

2) some local hardware stores corry it as it is used for grounding wire. It also is occasionally used to "wick" solder away from joins.

3) Newark Electronics sells what you want but you might have to buy 50-100
feet at about $30-$100. In Newark catalog 119, Beldon's braid is on page
1175 and Alpha's is on page 1239. Alpha calls theirs "tinned copper flat
braid". My Beldon braid # 8660 carried 14.5 amps and had a cross section
of about 3800 circular mils.

4) try hosfelt electronics. http://hosfelt.com/index.htm

5) Try McMaster Carr. http://McMaster.com

6) Ham radio folks often use braided wire as grounding wire.

7) This wire is often used in Physiology experiments where "noise" is often a problem. You might find it in a shop that repairs this equipment on your campus.

8) Try commercial dealers who sell High Vacuum equipment. They might be willing to sell some of the braid.

Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Thu Jul 4 09:59:07 2002



From: elbio martinez :      edmarti-at-ceride.gov.ar
Date: Thu, 04 Jul 2002 11:43:17 ART
Subject: XRF Detector Unit PV9500

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Hello all,
Please inform if theres someone who knows about the internal electronics of the detecting unit of a x-ray fluorescence which characteristics are:

EDAX PV9740/05

MODEL PV9500 165-17

ACTIVE AREA 10 MM2

AMPLIFIER MODEL 183 A

The problem is that some LED or PHOTODIODE of said detector burnt out. The detector is a vacuum packed unit and this diagnostic is the result of measurements on the detector connection pins guided by an electric circuit scheme.

If someone can give me advice, please contact me,



Tic. Ppal Elbio Martmnez
CERIDE - CONICET
G|emes 3450
3000 - Santa Fe
Argentina
email: edmarti-at-ceride.gov.ar


From daemon Thu Jul 4 10:04:20 2002



From: edmarti-at-crdsf2.arcride.edu.ar
Date: Thu, 4 Jul 2002 11:58:51 +0300
Subject: XRF Detector Unit PV9500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
Please inform if theres someone who knows about the internal electronics of the detecting unit of a x-ray fluorescence which characteristics are:

EDAX PV9740/05

MODEL PV9500 165-17

ACTIVE AREA 10 MM2

AMPLIFIER MODEL 183 A

The problem is that some LED or PHOTODIODE of said detector burnt out. The detector is a vacuum packed unit and this diagnostic is the result of measurements on the detector connection pins guided by an electric circuit scheme.

If someone can give me advice, please contact me,



Tic. Ppal Elbio Martmnez
CERIDE - CONICET
G|emes 3450
3000 - Santa Fe
Argentina
email: edmarti-at-ceride.gov.ar


From daemon Thu Jul 4 10:26:13 2002



From: =?iso-8859-1?Q?Elbio_Mart=EDnez?= :      edmarti-at-ceride.gov.ar
Date: Thu, 4 Jul 2002 12:20:05 -0300
Subject: XRF Detector Unit PV9500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello all,
Please inform if there's someone who knows about the internal electronics of
the detecting unit of a x-ray fluorescence which characteristics are:

EDAX PV9740/05

MODEL PV9500 165-17

ACTIVE AREA 10 MM2

AMPLIFIER MODEL 183 A

The problem is that some LED or PHOTODIODE of said detector burnt out. The
detector is a vacuum packed unit and this diagnostic is the result of
measurements on the detector connection pins guided by an electric circuit
scheme.

If someone can give me advice, please contact me,



Tic. Ppal Elbio Martmnez
CERIDE - CONICET
G|emes 3450
3000 - Santa Fe
Argentina
email: edmarti-at-ceride.gov.ar



From daemon Thu Jul 4 15:37:04 2002



From: Rogerio Lucio de Almeida - doutorado :      ralmeida-at-ifi.unicamp.br
Date: Thu, 4 Jul 2002 17:22:19 -0300 (BRT)
Subject: Looking for Abrikosov original article

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Hello All,
I'm looking for the original article of Abrikosov( in russian or in
english) in which he introduces
the Type-II superconductor. I can't find it on the internet. Does
anybody have the text or know where to look for it? Reference:
A.A. Abrikosov, Zh. Eksperim. i Teor. Fiz. 32, 1442 (1957) [Sov. Phys.
-- JETP 5, 1174 (1957)]

Thanks
R. Almeida











************************************
Rogerio Lucio de Almeida

















From daemon Thu Jul 4 17:09:43 2002



From: Ken Tiekotter :      tiekotte-at-up.edu (by way of MicroscopyListserver)
Date: Thu, 4 Jul 2002 15:00:15 -0700
Subject: Leaf Lumina or MicroLumina

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have or know where I can buy a Leaf Lumina or MicroLumina
camera. I understand the limitations of the technology, but for the price
I am hard pressed to find a camera of equal resolution/dynamic range.
Unless any one you have suggestions?

Thank you.
Ken

--
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

Tel.: 503-413-5391


From daemon Thu Jul 4 18:53:54 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 5 Jul 2002 11:44:34 GMT+1200
Subject: Re: XRF Detector Unit PV9500

Contents Retrieved from Microscopy Listserver Archives
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I guess you've already approached your friendly local Philips/EDAX
agent?

I may be wrong, but I've always thought that the manufacturer is in a
far better position to service these things than is anyone else.

Any interesting opinions out there?

cheers

rtch






} Date: Thu, 04 Jul 2002 11:43:17 ART
} From: elbio martinez {edmarti-at-ceride.gov.ar}
} Reply-to: edmarti-at-ceride.gov.ar
} Subject: XRF Detector Unit PV9500
} To: Microscopy-at-sparc5.microscopy.com

} --------------------------------------------------------------------
} ---- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
} ---.
}
}
} Hello all,
} Please inform if theres someone who knows about the internal
} electronics of the detecting unit of a x-ray fluorescence which
} characteristics are:
}
} EDAX PV9740/05
}
} MODEL PV9500 165-17
}
} ACTIVE AREA 10 MM2
}
} AMPLIFIER MODEL 183 A
}
} The problem is that some LED or PHOTODIODE of said detector burnt
} out. The detector is a vacuum packed unit and this diagnostic is the
} result of measurements on the detector connection pins guided by an
} electric circuit scheme.
}
} If someone can give me advice, please contact me,
}
}
}
} Tic. Ppal Elbio Martmnez
} CERIDE - CONICET
} G|emes 3450
} 3000 - Santa Fe
} Argentina
} email: edmarti-at-ceride.gov.ar
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Fri Jul 5 02:00:31 2002



From: Allen Sampson :      ars-at-sem.com
Date: Fri, 5 Jul 2002 01:53:31 -0700
Subject: RE: XRF Detector Unit PV9500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One would like to think so, but we're often disappointed in life.

I don't have the schematics on that particular unit, but the manufacturer
would. Whether they would want to share that or not is another question.
The item in question is probably used to reset the detector. Normally,
the components within the vacuum would be only the detector diode and the
FET used as a preamplifier, the remaining preamplifier parts are usually
outside of the vacuum in a small metal box attached to the detector.
However, even if they are inside the vacuum of the dewar cold finger
assembly, that can be opened, repaired and re-pumped - if you have the
right vacuum fitting. Again, the manufacturer could supply you with that,
if they so desire.

The problem with manufacturers is often three fold - they often have
difficulty holding on to good techs, their self-interest is often to sell
you the latest system and they often see others working on their
instruments (even needy customers) as cutting into their profits. A lot of
oftens that occasionally add up to what is often seen as poor customer
service.

Individuals will generally remain loyal to a manufacturer as long as they
are able to meet that person's needs. If you've had a good result there,
consider yourself lucky. At any particular time, there is at least one
manufacturer that can actually provide good service with a view of their
customer's needs. Unfortunately, none has ever kept that up for more than
a few years. A customer's best friend is a good, local, service tech who
stays with the manufacturer for a long time. Someone who has a talent for
the work, good knowledge of the instruments, experience in working on them
and the manufacturer's backing for parts and information, is a great asset.

I'm most aquainted with SEMS. Twenty years ago, ETEC, after being a market
leader for many years, decided to get out of the business. Within the last
couple of years, we've seen that again with Amray. Those are two extremes
that used one business as a stepping stone to another. But in the ensuing
years, every manufacturer out there has enjoyed periods of high sales, good
customer relations, eroding sales, shifts in thoughts of service as a
profit center rather than a vital part of design engineering and marketing
leading to increases in turn-over rate and reduced knowledge and experience
on the part of their service engineers.

In XRF, HNU comes to mind as an extreme of the first kind. Every other
manufacturer as examples of the latter.

In many ways, it is a matter of the life cycle experienced by all
businesses. This natural life cycle has recently become a factor in
thought processes on business and the stock market. It is true across the
board, no matter what sector a business is in. It is the reason every
business has to be constantly re-inventing itself.

Now, Ritchie, to the heart of the matter, at least in a market the size of
America. We have here technicians with decades of experience in such
instrumentation. Many of them (I try to keep track of them as referrals)
have gone into business for themselves. This is generally a desire to
continue the work they love, without interference from corporations whose
mergers, acquisitions and buy-outs continually dilute and obfuscate the
customer's needs.

I am happy to here state that I am one of those who left that melee over
twenty years ago. I have never really advertised my services, but have
survived in business on word of mouth and benign exposure on the internet.
In every case, my customers have sought me out. There is only one
possible reason for that - the manufacturer has not provided the value that
the customer expects. Frankly, there is no one more knowledgeable on what
manufacturers are doing wrong, but not one has come to ask me what I would
so willingly tell them.

I do make my share of mistakes, but I have more experience on more
different systems from more manufacturers than just about anyone out there.
But that breadth of experience, itself, brings real life practical
knowledge that no manufacturer's tech can equal with his or hers limited
experience and knowledge.

I've tooted my own horn enough, but actually, I've been tooting the horn of
other independents out there. When you need us, it's good to know we're
there. When you don't, you should be glad that there are others who do,
because, in our own small way, we keep the manufacturer's feet on the
ground. I could tell you some real horror stories of what manufacturers
have pulled when they think that they have no competition, but I'll save
that for another day.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Friday, July 05, 2002 4:45 AM, Ritchie Sims [SMTP:r.sims-at-auckland.ac.nz]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} I guess you've already approached your friendly local Philips/EDAX
} agent?
}
} I may be wrong, but I've always thought that the manufacturer is in a
} far better position to service these things than is anyone else.
}
} Any interesting opinions out there?
}
} cheers
}
} rtch
}
}
}
}
}
}
} } Date: Thu, 04 Jul 2002 11:43:17 ART
} } From: elbio martinez {edmarti-at-ceride.gov.ar}
} } Reply-to: edmarti-at-ceride.gov.ar
} } Subject: XRF Detector Unit PV9500
} } To: Microscopy-at-sparc5.microscopy.com
}
} } --------------------------------------------------------------------
} } ---- The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------
} } ---.
} }
} }
} } Hello all,
} } Please inform if theres someone who knows about the internal
} } electronics of the detecting unit of a x-ray fluorescence which
} } characteristics are:
} }
} } EDAX PV9740/05
} }
} } MODEL PV9500 165-17
} }
} } ACTIVE AREA 10 MM2
} }
} } AMPLIFIER MODEL 183 A
} }
} } The problem is that some LED or PHOTODIODE of said detector burnt
} } out. The detector is a vacuum packed unit and this diagnostic is the
} } result of measurements on the detector connection pins guided by an
} } electric circuit scheme.
} }
} } If someone can give me advice, please contact me,
} }
} }
} }
} } Tic. Ppal Elbio Martmnez
} } CERIDE - CONICET
} } G|emes 3450
} } 3000 - Santa Fe
} } Argentina
} } email: edmarti-at-ceride.gov.ar
} }
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}
}
}



From daemon Fri Jul 5 09:48:33 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Fri, 05 Jul 2002 16:37:05 +0200
Subject: Zeiss AxioCam HR

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,

Does anyone of you have some experience you want to share about the Zeiss
AxioCam HR? We are planning to buy one, but first I would like to know the
positiv and negativ things about it.
Thanks in advance,

Sven Terclavers



From daemon Fri Jul 5 10:28:47 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Fri, 5 Jul 2002 11:23:44 -0400
Subject: XRF Detector Unit PV9500

Contents Retrieved from Microscopy Listserver Archives
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does anyone where i can obtain spare parts for an agfa print processor agfa
3700, or where a used one can be purchased?
thanks
john


From daemon Fri Jul 5 11:38:52 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 5 Jul 2002 17:34:09 +0100 (GMT Daylight Time)
Subject: Re: RE: XRF Detector Unit PV9500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Fri, 5 Jul 2002 01:53:31 -0700 Allen Sampson
{ars-at-sem.com} wrote:
} The problem with manufacturers is often three fold - they often have
} difficulty holding on to good techs, their self-interest is often to sell
} you the latest system and they often see others working on their
} instruments (even needy customers) as cutting into their profits. A lot of
} oftens that occasionally add up to what is often seen as poor customer
} service.
}
} Individuals will generally remain loyal to a manufacturer as long as they
} are able to meet that person's needs. If you've had a good result there,
} consider yourself lucky. At any particular time, there is at least one
} manufacturer that can actually provide good service with a view of their
} customer's needs. Unfortunately, none has ever kept that up for more than
} a few years.

I wish to report that in the 25 years I have worked here we
have had continuous good service from a manufacturer.

During that time there have been a series of local
engineers most of whom have been replaced as they
progressed within the company. I do not feel that we have
ever been penalised for carrying out our own service,
indeed they have been helpful in supplying components,
information and even redundant items from scrapped
instruments to keep older instruments running. We have also
told them of alternative sources that we have found for
their repairs or components.

I accept that the situation may be different in other
parts of the world and that the original comments probably
refer to the USA but I wish to correct the impression that
manufacturers cannot keep up a high standard of service for
more than a few years. It is possible.

With any complex piece of equipment there is the
possiblilty of problems, the most important thing is for
the customer, the supplier and the service organisation to
have trust and faith in each other.

It takes hard work from all sides to build up this sort of
relationship, but it is worth it.

Ron
Disclaimer: I probably buy the engineers as many beers as
they buy me so I don't feel under any obligation.

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk





From daemon Fri Jul 5 16:28:25 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 05 Jul 2002 17:21:20 -0400
Subject: Re: XRF Detector Unit PV9500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ron,

Perhaps something about wanting to remain on good terms with a customer with the
visibility of Oxford University has something to do with it? A firm named
"Widget Tool Alloys" might be perceived somewhat differently by a manufacturer.

Certainly in the U.S. the attitude of many OEMs often becomes "That's a really
old model... I'm sure we don't have any documentation on that. Most of our
customers have migrated to the _____ platform". While that is probably true in
the case of rapidly changing data handling systems, it is less true of other
systems. In my experience this has been particularly galling when it comes to
mechanical systems (for which nothing short of abuse should cause them to wear
out) and for detector electronics where the the nature of the detection process
and the signal conditioning requirements remained static for about two decades.

Disclaimer: If Widget Tool Alloys actually exists out there somewhere, my
apologies. No disrespect is intended.

John Twilley

Ron Doole wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} On Fri, 5 Jul 2002 01:53:31 -0700 Allen Sampson
} {ars-at-sem.com} wrote:
} } The problem with manufacturers is often three fold - they often have
} } difficulty holding on to good techs, their self-interest is often to sell
} } you the latest system and they often see others working on their
} } instruments (even needy customers) as cutting into their profits. A lot of
} } oftens that occasionally add up to what is often seen as poor customer
} } service.
} }
} } Individuals will generally remain loyal to a manufacturer as long as they
} } are able to meet that person's needs. If you've had a good result there,
} } consider yourself lucky. At any particular time, there is at least one
} } manufacturer that can actually provide good service with a view of their
} } customer's needs. Unfortunately, none has ever kept that up for more than
} } a few years.
}
} I wish to report that in the 25 years I have worked here we
} have had continuous good service from a manufacturer.
}
} During that time there have been a series of local
} engineers most of whom have been replaced as they
} progressed within the company. I do not feel that we have
} ever been penalised for carrying out our own service,
} indeed they have been helpful in supplying components,
} information and even redundant items from scrapped
} instruments to keep older instruments running. We have also
} told them of alternative sources that we have found for
} their repairs or components.
}
} I accept that the situation may be different in other
} parts of the world and that the original comments probably
} refer to the USA but I wish to correct the impression that
} manufacturers cannot keep up a high standard of service for
} more than a few years. It is possible.
}
} With any complex piece of equipment there is the
} possiblilty of problems, the most important thing is for
} the customer, the supplier and the service organisation to
} have trust and faith in each other.
}
} It takes hard work from all sides to build up this sort of
} relationship, but it is worth it.
}
} Ron
} Disclaimer: I probably buy the engineers as many beers as
} they buy me so I don't feel under any obligation.
}
} ----------------------
} Mr. R.C. Doole
} Department of Materials,
} University of Oxford.
} Parks Road, Oxford. OX1 3PH. UK.
} Phone +44 (0) 1865 273701
} Fax +44 (0) 1865 283333
} ron.doole-at-materials.ox.ac.uk





From daemon Mon Jul 8 06:16:29 2002



From: Chris Peppiatt :      chris.peppiatt-at-nuigalway.ie
Date: Mon, 08 Jul 2002 11:57:21 +0100
Subject: Carbon Quantitation by EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

Someone has asked me to do some work for them on mineral specimens. The
samples will be mounted in resin and polished and we will then coat with
carbon. Obviously we will set our software to deconvolute carbon from the
analyses. We have the option to coat samples with either gold or silver and
then look at the carbon content. I suppose the questions I would like to
have answered are:

(1) Heavy elements like gold or silver will absorb some of the light
element (inc. carbon) X-rays when used as coatings. Is there any way of
correcting for this to get an accurate quantitative analysis of carbon content?

(2) Is there any way of removing either gold/silver or carbon coatings from
such samples except for the obvious method of regrinding/polishing the
coating off?

Thanks in advance.

Regards,

Chris Peppiatt


============================================
Dr. Chris Peppiatt (Experimental Officer),
The National Centre for Biomedical Engineering Science,
Science & Engineering Technology Building,
National University of Ireland Galway,
Galway City, Co. Galway,
Republic of Ireland.
chris.peppiatt-at-nuigalway.ie
Phone: +353 91 512157 Fax: +353 91 750596
=============================================



From daemon Mon Jul 8 06:26:30 2002



From: Chris Peppiatt :      chris.peppiatt-at-nuigalway.ie
Date: Mon, 08 Jul 2002 12:20:14 +0100
Subject: Carbon Quantitation by EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

Someone has asked me to do some work for them on mineral specimens. The
samples will be mounted in resin and polished and we will then coat with
carbon. Obviously we will set our software to deconvolute carbon from the
analyses. We have the option to coat samples with either gold or silver and
then look at the carbon content. I suppose the questions I would like to
have answered are:

(1) Heavy elements like gold or silver will absorb some of the light
element (inc. carbon) X-rays when used as coatings. Is there any way of
correcting for this to get an accurate quantitative analysis of carbon content?

(2) Is there any way of removing either gold/silver or carbon coatings from
such samples except for the obvious method of regrinding/polishing the
coating off?

Thanks in advance.

Regards,

Chris Peppiatt


============================================
Dr. Chris Peppiatt (Experimental Officer),
The National Centre for Biomedical Engineering Science,
Science & Engineering Technology Building,
National University of Ireland Galway,
Galway City, Co. Galway,
Republic of Ireland.
chris.peppiatt-at-nuigalway.ie
Phone: +353 91 512157 Fax: +353 91 750596
=============================================



From daemon Mon Jul 8 08:54:37 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 8 Jul 2002 09:39:33 -0400
Subject: Re: Leaf Lumina or MicroLumina

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Does anyone have or know where I can buy a Leaf Lumina or MicroLumina
} camera. I understand the limitations of the technology, but for the price
} I am hard pressed to find a camera of equal resolution/dynamic range.
} Unless any one you have suggestions?
}
} Thank you.
} Ken
}
} --
Ken, Check Electron Microscopy Sciences.
No financial interest, just a long-time customer.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Jul 8 09:05:20 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 8 Jul 2002 09:57:56 -0400
Subject: RE: copper braid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Debby,
When I had this problem, I went to Pep Boys [no stock or other
relationship] and purchased some stranded copper battery cable. I had to
'trim' off the connectors, but the solution was satisfactory. Another
choice is any electric supply store that sells to contractors or a 'Home
Depot' [no stock or other relationship], though may be more likely to find
stranded Al than Cu there. I have always found stranded Cu at one or the
other of these places. Contractor supplies and Home Depot are usually less
expensive, because the cable is sold by the foot. Such cable is used in
many high wattage situations.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Debby Sherman
} Reply To: Debby Sherman
} Sent: Wednesday, July 3, 2002 9:45 AM
} To: message to: MSA list
} Subject: copper braid
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers,
} I am trying to find a source for heavy copper braid wire to repair some
} accessories used for metal and carbon evaporation. This is the stiff but
} flexible wire that would go between the connectors in a high vacuum
} evaporator and, in this case, the carbon evaporation apparatus. Does
} anyone have a source in the USA who is likely to sell small quantities? I
} just need a couple of feet at most.
}
} Thanks,
} Debby
}
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
}
} Purdue University E-mail: dsherman-at-purdue.edu
}
} S-052 Whistler Building
} West Lafayette, IN 47907
}
}
}
}


From daemon Mon Jul 8 09:16:43 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 08 Jul 2002 09:07:54 -0500
Subject: Re: Carbon Quantitation by EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would be quite cautious in trying to directly measure carbon. We have
wanted to for years, but I tell clients not to expect much.

We can easily see carbon in carbonate minerals. I just do not try to
quantitate it directly. (The times I tried, I got very doubtful numbers. I
would like my results to be consistent within 50% relative error.) If you
are looking for carbon in lesser amounts, I am doubtful that it would work.

You are aware that the carbon is strongly absorbed by most other elements.
I understand that it is a big part of the problem. Even if you can get a
good carbon signal above background, you still have to deal with
uncertainty in the absorption coefficients.

There would also be problems if any of your resin smears over.

Maybe you can tell us a bit more what you would like to measure and we can
provide more information.

I recall a discussion a couple of months back about removing Au and Ag by
means other that polishing. It should be in the list archives.

Warren

At 11:57 AM 7/8/02 +0100, you wrote:
} Dear All,
}
} Someone has asked me to do some work for them on mineral specimens. The
} samples will be mounted in resin and polished and we will then coat with
} carbon. Obviously we will set our software to deconvolute carbon from the
} analyses. We have the option to coat samples with either gold or silver
} and then look at the carbon content. I suppose the questions I would like
} to have answered are:
}
} (1) Heavy elements like gold or silver will absorb some of the light
} element (inc. carbon) X-rays when used as coatings. Is there any way of
} correcting for this to get an accurate quantitative analysis of carbon content?
}
} (2) Is there any way of removing either gold/silver or carbon coatings
} from such samples except for the obvious method of regrinding/polishing
} the coating off?
}
} Thanks in advance.
}
} Regards,
}
} Chris Peppiatt

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Mon Jul 8 12:30:36 2002



From: Heeschen, Bill (WA) :      WAHEESCHEN-at-dow.com
Date: Mon, 8 Jul 2002 13:20:13 -0400
Subject: Retrofitting ElectroScan E-3 ESEM for fully automated control

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:
If any of you have experience with a major retrofit to an ElectroScan E-3
ESEM for fully automated control (stage, gas pressure, imaging, EDS, etc.),
I would like to have your thoughts - both good news and bad.
Thanks,
Bill

William A. Heeschen, Ph.D.
Microscopy, Digital Imaging
The Dow Chemical Company
Midland, Mi 48667
waheeschen-at-dow.com


From daemon Tue Jul 9 04:58:35 2002



From: John MacIsaac :      jmacisaac-at-coxhanson.ca
Date: Tue, 09 Jul 2002 06:50:31 -0300
Subject: Re: Frameborder (Out of Office)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am out of the office today, Monday, July 8, 2002.

Should you require immediate assistance, please contact my assistant, Gail Jensen, at (902) 421-6262, or by email at gjensen-at-coxhanson.ca.

} } } Microscopy 07/09/02 06:47 } } }

{HTML} {HEAD} {/HEAD} {BODY}
{iframe src=3Dcid:IQ6Pu6g60H37Us1 height=3D0 width=3D0}
{/iframe}
{FONT} {/FONT} {/BODY} {/HTML}


___________________ ATTENTION!! _____________________
A Virus Has Been Detected in the file attachment(s).
The file attachment(s) have been removed by Guinevere,
the Groupwise Antivirus Scanner.

====} You may wish to notify the sender!
_____________________________________________________


From daemon Tue Jul 9 08:05:55 2002



From: Tom Moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Tue, 09 Jul 2002 07:50:38 -0500
Subject: LKB Knife Makers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Upon some serious cleaning we have discovered three LKB glass knife makers
that we do not need to keep. We have one Model 7800 and two Model 7801 Type
As that are in good working order. If you are interested please reply to me
off line with an offer.
Tom

Thomas Moninger (thomas-moninger-at-uiowa.edu)
University of Iowa Central Microscopy Research Facility (www.uiowa.edu/~cemrf)
View expressed are my own.



From daemon Tue Jul 9 08:26:34 2002



From: Tom Moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Tue, 09 Jul 2002 08:17:22 -0500
Subject: LKB knife makers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Upon some serious cleaning we have discovered three LKB glass knife makers
that we do not need to keep. We have one Model 7800 and two Model 7801 Type
As that are in good working order. If you are interested please reply to me
off-line with an offer.
Tom

Thomas Moninger (thomas-moninger-at-uiowa.edu)
University of Iowa Central Microscopy Research Facility (www.uiowa.edu/~cemrf)
View expressed are my own.



From daemon Tue Jul 9 08:26:35 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 9 Jul 2002 09:17:57 -0400
Subject: FW: Gray, Microtomist's Formulary and Guide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Listers,

I am forwarding this as information about a volume that serves as a 'limited
access' ramp to the pre-1950's protocols and literature on staining and
microtechnique. The price I have been given for one is $84.50+. The other
relevant information is below in the remainder of the thread.

The last of Peter Gray is on the horizon. He has lasted longer than most.
This book has been discussed on the Histopathology Net List recently. You
can search at: http://www.histosearch.com/histonet.html

I have no business relationship with Krieger.

Regards,

Fred Monson

} ----------
} From: Krieger
} Sent: Monday, July 8, 2002 6:06 PM
} To: Monson, Frederick C.
} Subject: Re: Gray, Microtomist's Formulary and Guide
}
} Dear Dr. Monson:
} We are selling the remaining stock we have. At the present time we have
} approximately 113 copies available. If you are interested in purchasing
} large quantities (over 50 copies) we can offer you special discounts.
} Sincerely,
} Cheryl Stanton
} Krieger Publishing Company
} P.O. Box 9542
} Melbourne, FL 32902-9542
} Tel: (321) 724-9542
} Fax: (321-951-3671
} 1-800-724-0025
} E-mail: info-at-krieger-publishing
} www.krieger-publishing.com
}
} -----Original Message-----
} From: Monson, Frederick C. {fmonson-at-wcupa.edu}
} To: 'Krieger' {info-at-krieger-publishing.com}
} Date: July 08, 2002 1:52 PM
} Subject: RE: Gray, Microtomist's Formulary and Guide
}
}
} } Hi Cheryl,
} } I know that you are modifying your web site, but when I called up
} } your list of books, this one was NOT included. If I am to recommend the
} } book, I must know its current disposition. For example, do you consider
} it
} } a current, and future, publication? Are you simply selling off the last
} of
} } the last run?
} }
} } Thanks,
} }
} } Fred Monson
} }
} } } ----------
} } } From: Krieger
} } } Sent: Monday, July 8, 2002 11:47 AM
} } } To: Monson, Frederick C.
} } } Subject: Re: Gray, Microtomist's Formulary and Guide
} } }
} } } Dear Dr. Monson:
} } }
} } } Please be advised this book is available at a list price of $84.50
} plus
} } } $5.00 for shipping via Ground UPS. Please advise if you require
} further
} } } information.
} } }
} } } Sincerely,
} } } Cheryl Stanton
} } } Krieger Publishing Company
} } } P.O. Box 9542
} } } Melbourne, FL 32902-9542
} } } Tel: (321) 724-9542
} } } Fax: (321-951-3671
} } } 1-800-724-0025
} } } E-mail: info-at-krieger-publishing
} } } www.krieger-publishing.com
} } }
} } } -----Original Message-----
} } } From: Monson, Frederick C. {fmonson-at-wcupa.edu}
} } } To: 'info-at-krieger-publishing.com' {info-at-krieger-publishing.com}
} } } Date: July 08, 2002 11:35 AM
} } } Subject: Gray, Microtomist's Formulary and Guide
} } }
} } }
} } } } Is this one now out of print for good?
} } } }
} } } } Thanks for help,
} } } }
} } } } Frederick C. Monson, PhD
} } } } Center for Advanced Scientific Imaging
} } } } Schmucker II Science Center
} } } } West Chester University
} } } } South Church Street and Rosedale
} } } } West Chester, Pennsylvania, USA, 19383
} } } } Phone: 610-738-0437
} } } } FAX: 610-738-0437
} } } } fmonson-at-wcupa.edu
} } } } CASI URL: http://darwin.wcupa.edu/casi/
} } } } WCUPA URL: http://www.wcupa.edu/
} } } } Visitors URL: http://www.wcupa.edu/_visitors/
} } }
} } }
}
}


From daemon Tue Jul 9 10:44:09 2002



From: Maruthi Sridhar Balaji Bhaskar :      sb183-at-Ra.MsState.Edu
Date: Tue, 09 Jul 2002 10:34:43 -0500 (CDT)
Subject: SEM&LM - help needed for Metal localization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
Here is the problem we had. we are trying to localize the metals(Zn
and Cd) within the planttissues (Barley). Prior work has shown that if you fix
the tissue in FAA or gluteraldehyde-type fixes and continue with dehydration
steps, the metals leach or diffuse out of the plant into the fix or dehydrating
agent.Metal localization has mostly been done using cryofixation and with a
cryostage. As the cryostage is not avilable at our EM Center. We fixed the
samples conventionally (gluteraldehyde, ethanol dehydration)and cryofractured
in liquid N and subjected them to EDS system, but it could barely pick up the
metal in the tissue. but the analytical studies (ICP) showed theres enough
metal accumulation.
some of the alternatives we are thinking is-
Treating the tissue samples with chemicals to precipitate the metals and
continue with conventional procedures with minimum dehydration and fixation
steps.
Do any of you know of stains for metals (Zn& Cd) that would allow us to stain
the cryosections specifically for metals and view them by LM? Or do you have
any better or alternative suggestions?
We would appreciate any input and suggestions you may have.
thanks in advance
B.B.M.Sridhar
Graduate Research Assistant
Diagnostic Instrumentation and Analysis Laboratory (DIAL)
& Department of Forest Products
Mississippi State University
Starkville, MS -39759
Phone(office)- 662-325-9044


From daemon Tue Jul 9 10:44:20 2002



From: Maruthi Sridhar Balaji Bhaskar :      sb183-at-Ra.MsState.Edu
Date: Tue, 09 Jul 2002 10:37:40 -0500 (CDT)
Subject: SEM&LM - help needed for Metal localization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
Here is the problem we had. we are trying to localize the metals(Zn
and Cd) within the planttissues (Barley). Prior work has shown that if you fix
the tissue in FAA or gluteraldehyde-type fixes and continue with dehydration
steps, the metals leach or diffuse out of the plant into the fix or dehydrating
agent.Metal localization has mostly been done using cryofixation and with a
cryostage. As the cryostage is not avilable at our EM Center. We fixed the
samples conventionally (gluteraldehyde, ethanol dehydration)and cryofractured
in liquid N and subjected them to EDS system, but it could barely pick up the
metal in the tissue. but the analytical studies (ICP) showed theres enough
metal accumulation.
some of the alternatives we are thinking is-
Treating the tissue samples with chemicals to precipitate the metals and
continue with conventional procedures with minimum dehydration and fixation
steps.
Do any of you know of stains for metals (Zn& Cd) that would allow us to stain
the cryosections specifically for metals and view them by LM? Or do you have
any better or alternative suggestions?
We would appreciate any input and suggestions you may have.
thanks in advance
B.B.M.Sridhar
Graduate Research Assistant
Diagnostic Instrumentation and Analysis Laboratory (DIAL)
& Department of Forest Products
Mississippi State University
Starkville, MS -39759
Phone(office)- 662-325-9044


From daemon Tue Jul 9 15:51:47 2002



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Tue, 9 Jul 2002 16:12:38 -0400
Subject: Stop algae growth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recall that over the past couple of years several people have
inquired about ways to control the growth of algae in recirculating
cooling water systems.

I just received a catalog from an outfit called Home Improvements
that lists a device called 'Power Clear' for controlling algal growth
in garden pools and ponds. It is essentially a 15 x 5 x 5" chamber
with hose connections at each end. When the circulator's hose is
connected to it the circulating water flows over a quartz-sleeved
ultra violet bulb, which is said to kill parasites, mold spores,
bacteria and fungi in the water.

It sounds as though this would be very suitable to use in water
recirculators for electron microscopes and related instruments. The
cost is only about $130, with replacement bulbs priced at $30. Check
www.ImprovementsCatalog.com, or call 1-800-642-2112.

--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Tue Jul 9 16:06:23 2002



From: brandon.k.rice-at-uwrf.edu (by way of MicroscopyListserver)
Date: Tue, 9 Jul 2002 13:40:55 -0700
Subject: Ask-A-Microscopist: LM Questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (brandon.k.rice-at-uwrf.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, July
8, 2002 at 11:20:50
---------------------------------------------------------------------------

Email: brandon.k.rice-at-uwrf.edu
Name: Brandon Rice

Organization: University of Wisconsin-River Falls

Education: Undergraduate College

Location: River Falls, WI, United States

Question: I am using a .50 NA objective and three lenses to image 1.6
micron silica spheres onto a camera. The focal lengths of the three
lenses after the objective are 125mm, 38.1mm, and 90mm respectively.
I am utilizing Kohler illumination to illuminate the sample. The
image of the spheres appear very stretched out in the vertical
direction; the spheres look more like ellipses. I have attempted to
realign the objective and lenses over and over, but this does not
seem to affect the image. What do you recommend I try to make the
spheres look spherical?

---------------------------------------------------------------------------


From daemon Tue Jul 9 16:06:24 2002



From: Ann-Fook Yang :      yanga-at-agr.gc.ca (by way of MicroscopyListserver)
Date: Tue, 9 Jul 2002 13:47:21 -0700
Subject: question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My colleague asks:
Is it true or is a hoax that there is a software which makes it
possible to get focussed light microscopy images from pairs of under-
and overfocussed micrographs? If it si true, what is the name of the
software and where is it available?
Thank you.
M. Kalab

Please reply to:
scimat-at-magma.ca


From daemon Tue Jul 9 16:56:05 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Tue, 09 Jul 2002 14:46:23 -0400
Subject: Re: SEM&LM - help needed for Metal localization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 7/9/02 11:34 AM, Maruthi Sridhar Balaji Bhaskar at sb183-at-Ra.MsState.Edu
wrote:

} Here is the problem we had. we are trying to localize the metals(Zn
} and Cd) within the planttissues (Barley). Prior work has shown that if you
} fix
} the tissue in FAA or gluteraldehyde-type fixes and continue with dehydration
} steps, the metals leach or diffuse out of the plant into the fix or
} dehydrating
} agent.Metal localization has mostly been done using cryofixation and with a
} cryostage. As the cryostage is not avilable at our EM Center. We fixed the
} samples conventionally (gluteraldehyde, ethanol dehydration)and cryofractured
} in liquid N and subjected them to EDS system, but it could barely pick up the
} metal in the tissue. but the analytical studies (ICP) showed theres enough
} metal accumulation.
} some of the alternatives we are thinking is-
} Treating the tissue samples with chemicals to precipitate the metals and
} continue with conventional procedures with minimum dehydration and fixation
} steps.
} Do any of you know of stains for metals (Zn& Cd) that would allow us to stain
} the cryosections specifically for metals and view them by LM? Or do you have
} any better or alternative suggestions?
} We would appreciate any input and suggestions you may have.
} thanks in advance
Dear Maruthi,
Can you cryo-fix, then lyophylize at low temperatures (-90 C)? This
should retain the metals, but much of the structure will be hard to
visualize. If you can recognize the structures where the metals appear, you
can get a rough idea of the location of the metals, then try other
techniques to pin down the metals to better resolution if needed. Good
luck.
Yours,
Bill Tivol



From daemon Tue Jul 9 18:08:38 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Tue, 09 Jul 2002 18:49:09 +0200
Subject: Immunogold labeling with chicken =?iso-8859-1?Q?ab=B4s?=

Contents Retrieved from Microscopy Listserver Archives
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Has anybody out there experience with using chicken ab´s as primary ab´s
for immunogold labeling? Do they have disatvantages compared with
rabbit, mouse ab´s. I can remember that a couple of years ago there was
the word that the available anti-chicken-gold-conjugates are not as
"good" as anti-rabbit- or mouse-gold-conjugates.

Thanks for your help,

Stefan





°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Tue Jul 9 18:44:27 2002



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Wed, 10 Jul 2002 11:37:30 +1200
Subject: Re: Stop algae growth (plus pH control)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Wilbur, Thanks for the update

The recirculating cooling water systems problem that we have the most
problem trying to get a handle on is controlling the pH of the
cooling water to prevent corrosion. Since the end of the good old
days when we were allowed to use Sodium Chromate and Sodium
Bicarbonate we have not yet found a satisfactory replacement.

Satisfactory includes; effectiveness at pH control, life time before
depletion, safe handling and disposal, cost, availablility.

Trying to get good advice also seems to like the proverbial hens teeth.

Anyone out there come up with any good products recently.

Allan




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--
-------------------------------------------------
Allan Mitchell
Technical Manager
Otago Centre for Electron Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Tue Jul 9 18:50:19 2002



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Wed, 10 Jul 2002 11:43:12 +1200
Subject: Re: Stop algae growth (plus pH control)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Wilbur, Thanks for the update

The recirculating cooling water systems problem that we have the most
problem trying to get a handle on is controlling the pH of the
cooling water to prevent corrosion. Since the end of the good old
days when we were allowed to use Sodium Chromate and Sodium
Bicarbonate we have not yet found a satisfactory replacement.

Satisfactory includes; effectiveness at pH control, life time before
depletion, safe handling and disposal, cost, availablility.

Trying to get good advice also seems to like the proverbial hens teeth.

Anyone out there come up with any good products recently.

Allan




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
-------------------------------------------------
Allan Mitchell
Technical Manager
Otago Centre for Electron Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Tue Jul 9 19:10:43 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Tue, 09 Jul 2002 19:51:40 +0200
Subject: Immunogold labeling with hen =?iso-8859-1?Q?ab=B4s?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anybody out there experience with using chicken ab´s as primary ab´s

for immunogold labeling? Do they have disatvantages compared with
rabbit, mouse ab´s. I can remember that a couple of years ago there was
the word that the available anti-chicken-gold-conjugates are not as
"good" as anti-rabbit- or anti-mouse-gold-conjugates.

Thanks for your help,

Stefan





°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
c/o Rosenbaum Lab.
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6210

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Tue Jul 9 19:24:26 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Tue, 09 Jul 2002 20:04:58 +0200
Subject: TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have the following problem:
I am observing Epon sections, stained with uranyl acetate and lead
citrate in an Philips EM 201. The sections show a really severe
contamination with electron dense grains. These grains are always
associated with embedded structures (membranes, microtubules etc.).The
problem started after I changed the cathode. The contamination looks
kinda like lead-stain granularity and I think it has something to do
with the lead. A section stained with uranyl acetate only looks fine
(but I need the lead to get enough stain).
The crazy thing is that I don´t have the problem when I use our other
scope, a Zeiss EM 10. So if I take one of my sections and have a look at
it in the Zeiss EM 10, everything is perfect. The same section observed
in the Philips EM 201 shows this contamination with electron dense
grains. Both scopes operated at comparable conditions (80 kv, cold trap
etc.). So the contamination seems to be lead citrate and scope
dependend.
Anybody ever had that problem and might have an idea how to solve it?

Stefan



°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6210

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Tue Jul 9 19:39:55 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 09 Jul 2002 17:37:08 -0700
Subject: Sony video printer sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of US sources for the Sony
UD-890 and UD-895CE video printers? I'd
like to see if they would interface to NTSC
640x480 composite video.

Anyone using these? Comments?

Off-line please.

tnx,
gary g.



From daemon Tue Jul 9 19:52:28 2002



From: Larry Allard :      L2A-at-ornl.gov
Date: Tue, 09 Jul 2002 20:42:20 -0400
Subject: Fwd: Stop algae growth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This looks great...I'm going to try it out right away. The full link
for PowerClear (one word) is:

http://www.improvementscatalog.com/parent.asp?pf%5Fid=228244x&dept%5Fid=30&strFindSpec=powerclear&Solutions=&code=

Thanks Wil.

Larry






} Date: Tue, 09 Jul 2002 16:12:38 -0400
} From: Wil Bigelow {bigelow-at-engin.umich.edu}
} Subject: Stop algae growth
} X-Sender: bigelow-at-srvr5.engin.umich.edu
} To: Microscopy Listserver {microscopy-at-sparc5.microscopy.com}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Tue Jul 9 21:07:30 2002



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Tue, 9 Jul 2002 18:58:19 -0700
Subject: Administrivia: Double Postings...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

I've noticed a rash of double posting lately.
If you are getting an error message on your posting please
contact me. Do not simply try posting a second time.

Nestor
Your Friendly Neighborhood SysOp
--
Nestor J. Zaluzec
Argonne National Lab
Materials Science Division.


Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 630-252-7901


Home: Zaluzec-at-microscopy.com
Tel: 630-739-1160


From daemon Tue Jul 9 21:49:56 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Tue, 09 Jul 2002 22:50:54 -0400
Subject: Re: Stop algae growth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wil,

We looked into the use of UV sterilization (both those that generate ozone
and those that do not) for purposes of reducing or eliminating algae growth
in fountains containing artworks that are susceptible to staining or
corrosion by some of the chemical agents typically used for the same
purpose. I've also had to deal with the problem in closed recirculating
cooling systems. The problem is that the effects of UV are limited to the
flow-through cell and algae can still colonize the walls of the rest of the
system. When bits of the biofilm come off, they can block filter screens
and or the aperture that typically lies behind the anode of a fine-focus
X-ray tube. It doesn't matter that the debris got sterilized on its trip
through the UV lamp housing.

My experience with closed systems has been best when using only reverse
osmosis purified water with a very small amount of biocide. If there is no
source of minerals, the growth is retarded and cleaning may be limited to
three or four year intervals. Often, the nylon mesh of the intake filter
was the limiting factor, beginning to embrittle and give way, thereby
introducing its own problems.

John Twilley

Wil Bigelow wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I recall that over the past couple of years several people have
} inquired about ways to control the growth of algae in recirculating
} cooling water systems.
}
} I just received a catalog from an outfit called Home Improvements
} that lists a device called 'Power Clear' for controlling algal growth
} in garden pools and ponds. It is essentially a 15 x 5 x 5" chamber
} with hose connections at each end. When the circulator's hose is
} connected to it the circulating water flows over a quartz-sleeved
} ultra violet bulb, which is said to kill parasites, mold spores,
} bacteria and fungi in the water.
}
} It sounds as though this would be very suitable to use in water
} recirculators for electron microscopes and related instruments. The
} cost is only about $130, with replacement bulbs priced at $30. Check
} www.ImprovementsCatalog.com, or call 1-800-642-2112.
}
} --
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} 3062 Dow Bldg.; 2300 Hayward St.
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:734-763-4788; Ph:734-662-5237





From daemon Wed Jul 10 00:48:37 2002



From: Ronald Vane :      RVane-at-Evactron.com
Date: Monday, July 08, 2002 3:22 PM
Subject: Carbon Quantitation by EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chris and all:

Quantitative analysis of Carbon by EDX is nearly impossible because of the
very shallow penetration depth of the Carbon X-rays. You really just measure
the surface. Surface analysis techniques such as XPS and Auger also show
that thin carbon films love to form on surfaces. If you have an XPS you use
your ion gun to sputter off the carbon surface scum to see the real surface.

Dry ashing in a plasma cleaner can also remove the carbon surface layer.
Sputter etching can be used to remove gold and silver coatings.

Ron Vane
XEI Scientific
3124 Wessex Way, Redwood City, CA 94061
650-369-0133
www.SEMCLEAN.com

Note: XEI Scientific makes the EVACTRON plasma cleaning system for Electron
Microscope Chambers and FIBs, but does not make desk top plasma dry ashers
or sputter etchers.

-----Original Message-----
} From: Chris Peppiatt {chris.peppiatt-at-nuigalway.ie}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Wed Jul 10 01:40:03 2002



From: Anaspec :      anaspec-at-icon.co.za
Date: Wed, 10 Jul 2002 08:31:43 +0200
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Anaspec South Africa
anaspec-at-icon.co.za


From daemon Wed Jul 10 05:21:22 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 10 Jul 2002 12:12:08 +0200
Subject: Re: Carbon Quantitation by EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

In the same idee, what about mesurements on boron carbide ? Carbon AND
boron concentrations ! I see nice spectras, without anything else ( a bit
O, some times Al and N). And I have variations between samples in the B/C
ratio. In such a case can I mesure only ratio variation, or is it possible
to try some quantification (with standards). The sample is bulk B4C
and laser ablation thick layers (with dropplets). Primary energy 3 to 5
keV.

I think it's a bit pretentious to quantify. What is other's opinion ?



J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Tue, 9 Jul 2002, Ronald Vane wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Chris and all:
}
} Quantitative analysis of Carbon by EDX is nearly impossible because of the
} very shallow penetration depth of the Carbon X-rays. You really just measure
} the surface. Surface analysis techniques such as XPS and Auger also show
} that thin carbon films love to form on surfaces. If you have an XPS you use
} your ion gun to sputter off the carbon surface scum to see the real surface.
}
} Dry ashing in a plasma cleaner can also remove the carbon surface layer.
} Sputter etching can be used to remove gold and silver coatings.
}
} Ron Vane
} XEI Scientific
} 3124 Wessex Way, Redwood City, CA 94061
} 650-369-0133
} www.SEMCLEAN.com
}
} Note: XEI Scientific makes the EVACTRON plasma cleaning system for Electron
} Microscope Chambers and FIBs, but does not make desk top plasma dry ashers
} or sputter etchers.
}
} -----Original Message-----
} } From: Chris Peppiatt {chris.peppiatt-at-nuigalway.ie}
} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
} Date: Monday, July 08, 2002 3:22 PM
} Subject: Carbon Quantitation by EDX
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear All,
} }
} } Someone has asked me to do some work for them on mineral specimens. The
} } samples will be mounted in resin and polished and we will then coat with
} } carbon. Obviously we will set our software to deconvolute carbon from the
} } analyses. We have the option to coat samples with either gold or silver and
} } then look at the carbon content. I suppose the questions I would like to
} } have answered are:
} }
} } (1) Heavy elements like gold or silver will absorb some of the light
} } element (inc. carbon) X-rays when used as coatings. Is there any way of
} } correcting for this to get an accurate quantitative analysis of carbon
} content?
} }
} } (2) Is there any way of removing either gold/silver or carbon coatings from
} } such samples except for the obvious method of regrinding/polishing the
} } coating off?
} }
} } Thanks in advance.
} }
} } Regards,
} }
} } Chris Peppiatt
} }
} }
} } ============================================
} } Dr. Chris Peppiatt (Experimental Officer),
} } The National Centre for Biomedical Engineering Science,
} } Science & Engineering Technology Building,
} } National University of Ireland Galway,
} } Galway City, Co. Galway,
} } Republic of Ireland.
} } chris.peppiatt-at-nuigalway.ie
} } Phone: +353 91 512157 Fax: +353 91 750596
} } =============================================
} }
} }
} }
}
}



From daemon Wed Jul 10 05:34:09 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 10 Jul 2002 11:27:36 +0100 (GMT Daylight Time)
Subject: Re: TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When you take a "contaminated" section from the Philips
does it then look OK in the Zeiss?


Dave


On Tue, 09 Jul 2002 20:04:58 +0200 Stefan Geimer
{stefan.geimer-at-yale.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have the following problem:
} I am observing Epon sections, stained with uranyl acetate and lead
} citrate in an Philips EM 201. The sections show a really severe
} contamination with electron dense grains. These grains are always
} associated with embedded structures (membranes, microtubules etc.).The
} problem started after I changed the cathode. The contamination looks
} kinda like lead-stain granularity and I think it has something to do
} with the lead. A section stained with uranyl acetate only looks fine
} (but I need the lead to get enough stain).
} The crazy thing is that I don´t have the problem when I use our other
} scope, a Zeiss EM 10. So if I take one of my sections and have a look at
} it in the Zeiss EM 10, everything is perfect. The same section observed
} in the Philips EM 201 shows this contamination with electron dense
} grains. Both scopes operated at comparable conditions (80 kv, cold trap
} etc.). So the contamination seems to be lead citrate and scope
} dependend.
} Anybody ever had that problem and might have an idea how to solve it?
}
} Stefan
}
}
}
} °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} Stefan Geimer
} MCDB Dept.
} Yale University
} P.O. Box 208103
} New Haven, CT 06520-8103
} U.S.A.
}
} Tel.: 203/432-3473
} Fax.: 203/432-6210
}
} e-mail: stefan.geimer-at-yale.edu
} °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Jul 10 06:46:13 2002



From: Sarka Lhotak :      lhotaks-at-mcmail.cis.mcmaster.ca
Date: Wed, 10 Jul 2002 07:36:20 -0400 (EDT)
Subject: Re: question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dobry den, Milosi,
metoda se jmenuje "deconvolution" a je k mani v cele rade image
analysis softwares. Napr. Northern Eclipse od Empix Imaging a mnohe dalsi.
Jak je v Ottawe? Jeste pisete pro Neviditelneho Psa? Uz ho nectu, protoze
na to naveseli tolik reklam, ze to muj staricky pocitac nezvlada.
Mnoho pozdravu,
Sarka Lhotakova

On Tue, 9 Jul 2002, Ann-Fook Yang wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} My colleague asks:
} Is it true or is a hoax that there is a software which makes it
} possible to get focussed light microscopy images from pairs of under-
} and overfocussed micrographs? If it si true, what is the name of the
} software and where is it available?
} Thank you.
} M. Kalab
}
} Please reply to:
} scimat-at-magma.ca
}
}



From daemon Wed Jul 10 07:10:31 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Wed, 10 Jul 2002 08:03:52 -0400
Subject: Stop algae growth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wil, I would think this would kill the algae in the water and the UV chamber
but other areas are still vulnerable.
We made the mistake of installing a cooling water tap off our Haskris
system on an EM400 for digital camera cooling by using clear tubing. The
algae growth took off. I contacted Philips to determine how we could treat
the coolant to prevent this algae growth. They recommended a 50 / 50, water
/ ethylene glycol. We did this and had no further problems with algae even
with the clear tubing in place. I would not do this without the OK from you
scope vendor.
Russ Gillmeister
Xerox
~~~~~~~~~~~~~

-----Original Message-----
} From: Wil Bigelow [mailto:bigelow-at-engin.umich.edu]
Sent: Tuesday, July 09, 2002 4:13 PM
To: Microscopy Listserver


I recall that over the past couple of years several people have
inquired about ways to control the growth of algae in recirculating
cooling water systems.

I just received a catalog from an outfit called Home Improvements
that lists a device called 'Power Clear' for controlling algal growth
in garden pools and ponds. It is essentially a 15 x 5 x 5" chamber
with hose connections at each end. When the circulator's hose is
connected to it the circulating water flows over a quartz-sleeved
ultra violet bulb, which is said to kill parasites, mold spores,
bacteria and fungi in the water.

It sounds as though this would be very suitable to use in water
recirculators for electron microscopes and related instruments. The
cost is only about $130, with replacement bulbs priced at $30. Check
www.ImprovementsCatalog.com, or call 1-800-642-2112.

--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Wed Jul 10 07:43:45 2002



From: Gilles Grondin :      Gilles.Grondin-at-USherbrooke.ca
Date: Wed, 10 Jul 2002 08:35:31 -0400
Subject: iron detection.....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are trying to localize iron in yeast with light and electron microscopy
. Has anybody know of stains for iron at the light microscope and also for
electron microscope.

We would appreciate any input and suggestions you may have. Thanks for your
help,
Gilles




From daemon Wed Jul 10 07:59:47 2002



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 10 Jul 2002 08:52:07 -0400
Subject: Design

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If there is anyone out there who has recently designed a new EM lab in new
construction, I would appreciate hearing what special instructions you gave
to the architects and engineers to assure the appropriate environmental
conditions for operation of the instruments.

Thanks, Greg
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/EM


From daemon Wed Jul 10 08:07:36 2002



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 10 Jul 2002 09:00:19 -0400
Subject: Design

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



If there is anyone out there who has recently designed a new EM lab in new
construction, I would appreciate hearing what special instructions you gave
to the architects and engineers to assure the appropriate environmental
conditions for operation of the instruments.

Thanks, Greg


Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/EM


From daemon Wed Jul 10 09:18:52 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 10 Jul 2002 08:18:13 -0600
Subject: question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The answer is yes, actually several times.

1) Deconvolution. This technique takes several images (a stack) of images
taken at different focus positions, and calculates the "blurring" in each
image and finally removes it for a focused image. There are different types
of deconvolution (no neighbor, nearest neighbor, blind iterative). These
algorithms are fairly computation intense and can take a while to complete,
especially on large images. You also need information about the microscope
to get the best results.

2) Extended Focal Imaging (we call it that way, other manufacturers have
different names). This is a simpler approach, where the software simply
"collects" the focused parts from each image and combines them into a new
image. This usually works faster than deconvolution and you don't need any
other information from the microscope, but there might be artifacts in areas
where you find no structure on the image.

If you want to get more information, you can check "deconvolution" on the
internet, or go to our web site and look for "ride" (rapid image
deconvolution) and EFI (Extended Focal Imaging). As I mentioned above, other
manufacturers have similar software.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Ann-Fook Yang [mailto:yanga-at-agr.gc.ca]
Sent: Tuesday, July 09, 2002 2:47 PM
To: microscopy-at-sparc5.microscopy.com


My colleague asks:
Is it true or is a hoax that there is a software which makes it
possible to get focussed light microscopy images from pairs of under-
and overfocussed micrographs? If it si true, what is the name of the
software and where is it available?
Thank you.
M. Kalab

Please reply to:
scimat-at-magma.ca


From daemon Wed Jul 10 10:11:14 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 10 Jul 2002 11:03:44 +0200
Subject: follow up: TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I tried a lot of stuff to solve my "pepper" problem either on the "stainig
side", like shorter lead stain, different batch of lead citrate, prolonged
washes after fix and osmium, I don´t use phosphate buffers, etc, etc or on the
scope side (Philips EM 201) like using different objektive/condensor
apertures, low kv, low emission, etc. etc. Nothing helped.

If I take a section I worked with a couple of weeks ago (and then it was ok in
the Philips EM 20, I took dozens of nice negs) and put it in the same Philips
EM 201 now (exactly the same scope settings) I get this "pepper" and it is
really bad. If I take such a "peppered" section to the Zeiss EM 10 I still can
see the "pepper", so it is not a problem of not being able to see the
contamination in the Zeiss.

It drives me crazy.

Stefan



°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6210

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Wed Jul 10 10:37:19 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 10 Jul 2002 11:28:32 -0400
Subject: RE: TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


But do you have the problem with a section that is taken from the Philips to
the Zeiss?

Thanks,

Fred Monson

} ----------
} From: Stefan Geimer
} Sent: Tuesday, July 9, 2002 2:04 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM, Epon sections, problem with "pepper"
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have the following problem:
} I am observing Epon sections, stained with uranyl acetate and lead
} citrate in an Philips EM 201. The sections show a really severe
} contamination with electron dense grains. These grains are always
} associated with embedded structures (membranes, microtubules etc.).The
} problem started after I changed the cathode. The contamination looks
} kinda like lead-stain granularity and I think it has something to do
} with the lead. A section stained with uranyl acetate only looks fine
} (but I need the lead to get enough stain).
} The crazy thing is that I don´t have the problem when I use our other
} scope, a Zeiss EM 10. So if I take one of my sections and have a look at
} it in the Zeiss EM 10, everything is perfect. The same section observed
} in the Philips EM 201 shows this contamination with electron dense
} grains. Both scopes operated at comparable conditions (80 kv, cold trap
} etc.). So the contamination seems to be lead citrate and scope
} dependend.
} Anybody ever had that problem and might have an idea how to solve it?
}
} Stefan
}
}
}
} °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} Stefan Geimer
} MCDB Dept.
} Yale University
} P.O. Box 208103
} New Haven, CT 06520-8103
} U.S.A.
}
} Tel.: 203/432-3473
} Fax.: 203/432-6210
}
} e-mail: stefan.geimer-at-yale.edu
} °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
}
}
}
}


From daemon Wed Jul 10 10:58:00 2002



From: Phil Fraundorf :      FraundorfP-at-umsl-mail02.umsl.edu
Date: Wed, 10 Jul 2002 10:50:14 -0500
Subject: Re: Design

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Dear Greg,

I'm not sure what your design goals are, but the following
may give you some ideas...

We designed a lab, with focus on atom-resolution TEM and
SPM, to occupy half of the first floor of a 3 story building.
There was a writeup about it in the November 1999 issue (vol
4 no 11) of Laboratory Design. A brief quote from the article
said: "After reviewing geotechnical studies, designers
decided to place the electron microscopy lab on the first
floor, as far away as possible from the arterial road.
Mechanical equipment, as well as the microscope's generator,
were located on the first floor in the office wing, which is
separated from the lab block by a 2-in. seismic joint. A
second 2-in. expansion joint isolates the lab wing from the
adjacent Benton Hall. The microscopy lab rests on a dedicated
2-ft-thick concrete foundation, with theater-wall construction
further mitigating vibration." I'm not sure what they mean
here by "the microscope's generator", but the elastic barriers
they describe extend through all 3+1 floors of the facility.

Electrical wiring and air handling systems were designed
to minimize stray fields and asymmetric convection around
electron microscope columns, separate air conditioning
controls supplied the HREM room (allowing shut-down to test
it's effects on vibration if necessary) along with a ceiling
crane for column disassembly and a wall feedthrough
allowing the scope's low voltage power supplies to be located
in an adjacent, vibrationally separated, area. Hall closets
for chillers, transformers, etc. were located well away from
more sensitive instruments. Dust generating areas (e.g.
specimen prep) were located away from the scope rooms, and
specimen handling areas were given single-color floor tiles
to make small dropped objects easier to find.

Lastly, since we knew we couldn't do much after the fact
if building design goals were compromised during construction,
we made it a practice to attend weekly construction meetings,
and to schedule vibration isolation tests at appropriate times
during construction. I remember painfully one in particular,
when the only way we could figure how to measure vibration
attenuation between the 2-foot thick slab, and the newly-
poured floor surrounding, was for me to jump down from a
4-foot retaining wall onto the new floor hitting with maximum
impact (that was the bad part) while others monitored on-slab
and off-slab vibration. We got numbers for amplitude
attentuation (I think somewhere between a factor of 10 and
100, in the 10 Hz frequency range), but the next day I could
barely get out of bed. Of course, it was less the results
of the tests than the fact that everyone knew we were actively
doing them that, we felt, worked in our favor.

Most elements of the strategy worked (provided I don't
mention light leaks in the darkroom). Under normal contact
mode operating conditions sitting out in the room on a
vibration table, our Nanoscope III SPM has stationary-tip
vibration amplitudes of about half an Angstrom, and our
300 kV Philips SuperTwin continues to reliably deliver
contrast in the sub-2A range even on amorphous materials
using the lowest possible bias setting. If you have further
questions or would like more details, contact me off of the
list server.

Cheers. /pf

Phil Fraundorf
UM-StL Physics and Astronomy
St. Louis MO 63121
office: (314)516-5044
lab: (314)516-5024
fax: (314)516-6152
http://www.umsl.edu/~fraundor

*********** REPLY SEPARATOR ***********

On 7/10/2002 at 9:00 AM you? wrote:

} If there is anyone out there who has recently designed a new EM lab in new
} construction, I would appreciate hearing what special instructions you
} gave
} to the architects and engineers to assure the appropriate environmental
} conditions for operation of the instruments.
}
} Thanks, Greg
}
} Gregory W. Erdos, Ph.D.
} Assistant Director, Biotechnology Program
} Scientific Director, ICBR EM CORE
} University of Florida Ph. 352-392-1295
} PO Box 118525 Fax 352-846-0251
} Gainesville, FL 32611 http://www.biotech.ufl.edu/EM






From daemon Wed Jul 10 12:40:43 2002



From: Emmanuelle Roux :      eroux-at-caprion.com
Date: Wed, 10 Jul 2002 13:32:03 -0400
Subject: Immunogold with hen Ab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Stephan,
I haven't yet worked (soon but not yet, so I can't tell you much about protocoles) with IgY but I can tell you where to buy secondary Ab to IgY conjugated to gold: BIO/CAN Scientific (Jackson) 1-800-387-8125 or 905-828-2455
They are located in Ontario, Canada.
Donkey anti-chicken IgY - 6 nm cat# 703--195-155
Donkey anti-chicken IgY - 12 nm cat# 703--205-155
Donkey anti-chicken IgY - 18 nm cat# 703--215-155
Hope it helps

Emmanuelle



Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620



From daemon Wed Jul 10 12:44:56 2002



From: Emmanuelle Roux :      eroux-at-caprion.com
Date: Wed, 10 Jul 2002 13:39:01 -0400
Subject: Immunogold with hen Ab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hi Stephan,
} I haven't yet worked (soon but not yet, so I can't tell you much about protocoles) with IgY but I can tell you where to buy secondary Ab to IgY conjugated to gold: BIO/CAN Scientific (Jackson) 1-800-387-8125 or 905-828-2455
} They are located in Ontario, Canada.
} Donkey anti-chicken IgY - 6 nm cat# 703--195-155
} Donkey anti-chicken IgY - 12 nm cat# 703--205-155
} Donkey anti-chicken IgY - 18 nm cat# 703--215-155
} Hope it helps
}
} Emmanuelle
}
}
}
} Emmanuelle Roux, PhD
} Senior Scientist
} Caprion Pharmaceuticals
} 7150 Alexander Fleming
} St-Laurent, H4S 2C8
} Quebec, Canada
} Tel: 514-940-3600 ext. 3773
} Fax: 514-940-3620
}


From daemon Wed Jul 10 12:47:42 2002



From: MARK JEFFREY TALBOT :      mark.talbot-at-studentmail.newcastle.edu.au (by
Date: Wed, 10 Jul 2002 10:28:02 -0700
Subject: Congo Red

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I have a little problem. I am staining thin sections of LR White
embedded plant tissue with a 1% aqueous solution of Congo Red (Sigma)
for 1 min, with three 'copious' washes from a distilled water wash
bottle. Instead of the cell wall labelling, I get a 'negative' image of
the wall, and the cytoplasm stains strongly. I don't know why this is
so; I made up a mew batch of the stain, but that didn't improve the
labelling, and it's never happened before. I checked the pH, and it's
around 9. Am I going mad, or is there something about Gongo Red that I
should know about?

Thanks for you help in advance,


Mark.


Mark Talbot
Department of Biological Sciences
University of Newcastle
Callaghan NSW 2308
AUSTRALIA


From daemon Wed Jul 10 13:08:22 2002



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Wed, 10 Jul 2002 19:02:49 +0100
Subject: Re: Carbon Quantitation by EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am curious to hear the answer to this question from Jacques and I have
something to add.
Once I performed EDS analyses on a sample of BC particles on C film in order to
test a new EDS analysis system installed on a new FEG 200KeV TEM. After selecting
the correct time constant in the EDS software I could get nice distinguishable C
and B peaks. Later I tried doing a similar EDS analysis on large FeB2 particles in

a Fe matrix. I mention large here to stress that the particles went through the
specimen foil so most of the X-rays were being emitted directly from the particles

without passing through the Fe matrix before reaching the detector. I never saw
even a hint of a B peak. These ppts should have contained 33at%B.
Was I doing something wrong or is that an indication of how easily boron X-rays
are absorbed within a sample containing large z atoms?

Faerber Jacques wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi all
}
} In the same idee, what about mesurements on boron carbide ? Carbon AND
} boron concentrations ! I see nice spectras, without anything else ( a bit
} O, some times Al and N). And I have variations between samples in the B/C
} ratio. In such a case can I mesure only ratio variation, or is it possible
} to try some quantification (with standards). The sample is bulk B4C
} and laser ablation thick layers (with dropplets). Primary energy 3 to 5
} keV.
}
} I think it's a bit pretentious to quantify. What is other's opinion ?
}
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces)
} 23, rue de Loess
} 67037 Strasbourg CEDEX
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)0 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
}
} On Tue, 9 Jul 2002, Ronald Vane wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Chris and all:
} }
} } Quantitative analysis of Carbon by EDX is nearly impossible because of the
} } very shallow penetration depth of the Carbon X-rays. You really just measure
} } the surface. Surface analysis techniques such as XPS and Auger also show
} } that thin carbon films love to form on surfaces. If you have an XPS you use
} } your ion gun to sputter off the carbon surface scum to see the real surface.
} }
} } Dry ashing in a plasma cleaner can also remove the carbon surface layer.
} } Sputter etching can be used to remove gold and silver coatings.
} }
} } Ron Vane
} } XEI Scientific
} } 3124 Wessex Way, Redwood City, CA 94061
} } 650-369-0133
} } www.SEMCLEAN.com
} }
} } Note: XEI Scientific makes the EVACTRON plasma cleaning system for Electron
} } Microscope Chambers and FIBs, but does not make desk top plasma dry ashers
} } or sputter etchers.
} }
} } -----Original Message-----
} } } From: Chris Peppiatt {chris.peppiatt-at-nuigalway.ie}
} } To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
} } Date: Monday, July 08, 2002 3:22 PM
} } Subject: Carbon Quantitation by EDX
} }
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear All,
} } }
} } } Someone has asked me to do some work for them on mineral specimens. The
} } } samples will be mounted in resin and polished and we will then coat with
} } } carbon. Obviously we will set our software to deconvolute carbon from the
} } } analyses. We have the option to coat samples with either gold or silver and
} } } then look at the carbon content. I suppose the questions I would like to
} } } have answered are:
} } }
} } } (1) Heavy elements like gold or silver will absorb some of the light
} } } element (inc. carbon) X-rays when used as coatings. Is there any way of
} } } correcting for this to get an accurate quantitative analysis of carbon
} } content?
} } }
} } } (2) Is there any way of removing either gold/silver or carbon coatings from
} } } such samples except for the obvious method of regrinding/polishing the
} } } coating off?
} } }
} } } Thanks in advance.
} } }
} } } Regards,
} } }
} } } Chris Peppiatt
} } }
} } }
} } } ============================================
} } } Dr. Chris Peppiatt (Experimental Officer),
} } } The National Centre for Biomedical Engineering Science,
} } } Science & Engineering Technology Building,
} } } National University of Ireland Galway,
} } } Galway City, Co. Galway,
} } } Republic of Ireland.
} } } chris.peppiatt-at-nuigalway.ie
} } } Phone: +353 91 512157 Fax: +353 91 750596
} } } =============================================
} } }
} } }
} } }
} }
} }

--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Wed Jul 10 13:27:23 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 10 Jul 2002 14:20:41 -0400
Subject: Re: iron detection.....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For the LM either "Prussian Blue" reaction or "Turnbull's Blue" reaction depending on whether your
iron is +3 (ferric) or +2 (ferros). Theses are very simple and in any histotechnique text (ferro- or
ferri- cyanide in HCl) . You should run a control for verification. At the EM level I would think that
iron deposits would be electron dense and not require any staining. In fact, they might be more visible in
an unstained section.

Gilles Grondin wrote:

} We are trying to localize iron in yeast with light and electron microscopy
} . Has anybody know of stains for iron at the light microscope and also for
} electron microscope.
}
} We would appreciate any input and suggestions you may have. Thanks for your
} help,
} Gilles

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Jul 10 14:30:29 2002



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 10 Jul 2002 14:26:13 -0500
Subject: RE: TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have to ask the obvious, just to make sure that your staining technique is
meticulous.

1. are you doing the lead citrate stain in a low Co2 environment? KOH,
sucks up CO2 a lot better than NaOH... so try that... in a glass petri dish.

2. Use ultra clean boiled water to make up the lead citrate stain to drive
out CO2.

3. Wash the living hell out of the grids after staining. I bang them up
and down 60 times in half a liter of ultra pure water in 3 separate beakers,
after every stain. [I can stain about 35 grids on one of those rubber grid
holders with the slits in them to hold the grids... they are nice. You can
cut more slits to hold even more grids.]

4. Never use the lead citrate near the bottom of the vial. Every time I
try that, I end up with peppering, and make sure that your stain is fresh.

The reason that you don't see the artifact in one microscope probably just
means that you dont' have the same contrast between the 2 machines... maybe
different obj. aperture sizes or a lot of other reasons. But trust me, it's
probably still there.



From daemon Wed Jul 10 14:36:06 2002



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 10 Jul 2002 14:34:17 -0500
Subject: RE: follow up: TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have to ask the obvious, just to make sure that your staining technique is
meticulous.

1. are you doing the lead citrate stain in a low Co2 environment? KOH,
sucks up CO2 a lot better than NaOH... so try that... in a glass petri dish.

2. Use ultra clean boiled water to make up the lead citrate stain to drive
out CO2.

3. Wash the living hell out of the grids after staining. I bang them up
and down 60 times in half a liter of ultra pure water in 3 separate beakers,
after every stain. [I can stain about 35 grids on one of those rubber grid
holders with the slits in them to hold the grids... they are nice. You can
cut more slits to hold even more grids.]

4. Never use the lead citrate near the bottom of the vial. Every time I
try that, I end up with peppering, and make sure that your stain is fresh.

The reason that you don't see the artifact in one microscope probably just
means that you dont' have the same contrast between the 2 machines... maybe
different obj. aperture sizes or a lot of other reasons. But trust me, it's
probably still there.



From daemon Wed Jul 10 14:45:42 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 10 Jul 2002 14:38:35 -0500
Subject: SEM: Removing cell membranes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I seem to recall reading somewhere about a method of removing the top cell membranes from a cell culture layer. It seems to have involved placing a layer of material over the cultured cells and stripping it away, but I can't remember anything about where I saw the reference.

Can somebody help jog my memory? Or did I dream this? I figure if ANYONE knows, you folks will!

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Wed Jul 10 14:56:30 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Wed, 10 Jul 2002 15:50:06 -0400
Subject: RE: Silver enhancement of gold particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

Has anyone out there ever silver enhanced 20nm gold particles? I would like
to see by light microscopy the distribution in rat lung of inhaled gold
particles. I have done 1nm gold particle enhancement but that was for EM
viewing. Would it involve the same methodology?

Thanks!

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954



From daemon Wed Jul 10 15:30:29 2002



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 10 Jul 2002 15:27:18 -0500
Subject: RE: TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have to ask the obvious, just to make sure that your staining technique is
meticulous.

1. are you doing the lead citrate stain in a low Co2 environment? KOH,
sucks up CO2 a lot better than NaOH... so try that... in a glass petri dish.

2. Use ultra clean boiled water to make up the lead citrate stain to drive
out CO2.

3. Wash the living hell out of the grids after staining. I bang them up
and down 60 times in half a liter of ultra pure water in 3 separate beakers,
after every stain. [I can stain about 35 grids on one of those rubber grid
holders with the slits in them to hold the grids... they are nice. You can
cut more slits to hold even more grids.]

4. Never use the lead citrate near the bottom of the vial. Every time I
try that, I end up with peppering, and make sure that your stain is fresh.

The reason that you don't see the artifact in one microscope probably just
means that you dont' have the same contrast between the 2 machines... maybe
different obj. aperture sizes or a lot of other reasons. But trust me, it's
probably still there.



From daemon Wed Jul 10 16:24:51 2002



From: Vetrano, John S :      john.vetrano-at-pnl.gov
Date: Wed, 10 Jul 2002 14:15:41 -0700
Subject: TEM/SEM Tech. Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all;

the following is copied from our HR group announcement. The official bits
are listed below. I can answer some questions but I'm not the decision
maker so please don't bombard me with emails!

cheers, JSV
******************

Would you like to work at a National Laboratory? The Pacific Northwest
National Laboratory is looking for a Microscopist. If you are interested,
please apply by visiting our website:
http://jobs.pnl.gov/jobs.asp?req=104165
PNNL is an EEO/AA employer and values diversity in the workplace. F/M/D/V
are encouraged to apply.

Materials Interfaces and Characterization
Materials Science Division
Science & Engineering Associate II
Min. Salary: 40K/yr - Max. Salary: 59K/yr (salary is commensurate with
education and experience).

This position requires experience in electron microscopy and a 4-year degree
in a field of science or its equivalent. Background should be in metal and
ceramic sample preparation and in the operation of transmission and scanning
electron microscopes. Working experience with the use of analytical
electron microscopes is highly desired. Background in the handling,
preparation and examination of materials is also required. Will lead
technical activities dealing with material preparation and characterization
for PNNL scientists and engineers in support of a variety of projects within
the technical group. Will be responsible for performing analytical
characterization of materials, using transmission and scanning electron
microscopy. Will contribute research data for publications in refereed
technical journals and in reports to various sponsors. Excellent oral and
written communication skills and ability to establish positive working
relationships with other technical staff is required. This position will
report to the Materials Interfaces and Characterization Technical Group
Manager.
***********************


********
John S. Vetrano
Sr. Research Scientist
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0724 Fax: (509)376-6308
Email: mailto:john.vetrano-at-pnl.gov


From daemon Wed Jul 10 16:34:05 2002



From: Vetrano, John S :      john.vetrano-at-pnl.gov
Date: Wed, 10 Jul 2002 14:26:00 -0700
Subject: TEM/SEM Tech. Position Open

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} Hi all;
}
} the following is copied from our HR group announcement. The official bits
} are listed below. I can answer some questions but I'm not the decision
} maker so please don't bombard me with emails!
}
} cheers, JSV
} ******************
}
} Would you like to work at a National Laboratory? The Pacific Northwest
} National Laboratory is looking for a Microscopist. If you are interested,
} please apply by visiting our website:
} http://jobs.pnl.gov/jobs.asp?req=104165
} PNNL is an EEO/AA employer and values diversity in the workplace. F/M/D/V
} are encouraged to apply.
}
} Materials Interfaces and Characterization
} Materials Science Division
} Science & Engineering Associate II
} Min. Salary: 40K/yr - Max. Salary: 59K/yr (salary is commensurate with
} education and experience).
}
} This position requires experience in electron microscopy and a 4-year
} degree in a field of science or its equivalent. Background should be in
} metal and ceramic sample preparation and in the operation of transmission
} and scanning electron microscopes. Working experience with the use of
} analytical electron microscopes is highly desired. Background in the
} handling, preparation and examination of materials is also required. Will
} lead technical activities dealing with material preparation and
} characterization for PNNL scientists and engineers in support of a variety
} of projects within the technical group. Will be responsible for
} performing analytical characterization of materials, using transmission
} and scanning electron microscopy. Will contribute research data for
} publications in refereed technical journals and in reports to various
} sponsors. Excellent oral and written communication skills and ability to
} establish positive working relationships with other technical staff is
} required. This position will report to the Materials Interfaces and
} Characterization Technical Group Manager.
} ***********************
}
}
} ********
} John S. Vetrano
} Sr. Research Scientist
} Pacific Northwest National Laboratory
} MSIN P8-16
} P.O. Box 999
} Richland, WA 99352
} Phone: (509)372-0724 Fax: (509)376-6308
} Email: mailto:john.vetrano-at-pnl.gov
}


From daemon Wed Jul 10 16:59:04 2002



From: R. Ann Bliss :      bliss5-at-llnl.gov
Date: Wed, 10 Jul 2002 14:52:25 -0700
Subject: Replacement parts

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Dear Listers:

If anyone out there has a Kevex Analyst 8000 in "excess storage" we
would be interested in a pulse processor (4460). Who knows? We may be
looking for other modules in the near future. Please contact me
offline.

Warm Regards,
Annie
--

+++++++++++++++++++++++++++++

R. Ann Bliss
Technician, Chemistry and Materials Science
Materials Science and Technology Division
Lawrence Livermore National Laboratory

_____________________________


From daemon Wed Jul 10 18:24:23 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Wed, 10 Jul 2002 19:12:58 -0400
Subject: TMC Isolation Platform

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We have a TMC isolation platform for a TEM that we wish to sell as our new
scope will not fit on this platform. Currently used for a JEOL 100C. Unit
purchased in 1994 and in excellent condition. Interested parties should
contact

Joe Goodhouse
Confocal / EM Core Laboratory Manager
Department of Molecular Biology
Princeton University
609-258-5432
jgoodhouse-at-molbio.princeton.edu

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Wed Jul 10 18:54:14 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 10 Jul 2002 19:53:01 -0700
Subject: Re: SEM: Removing cell membranes

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HI Randy,
We've done this with cell cancer lines incubated onto a bone
surface or with bacteria incubated on a polished planchet of
hornblende. I placed double stickey C tabs cut into thin slivers onto an
alumium stub, remove the tab cover, inverted the aluminum, stub with the
exposed sticky +conductive surface over a dried /coated and previously
imaged sample, touching it lightly to the surface before pulling it
away---the idea being to Au/Pd coat both stubs, and then check them for
the cells, cell content or for the pit formed when the cells anchored into
the layer of bone / hornblende. As might be expected, I had fewer problems
with the hornblende surface than with the bone slice.
I hope that your ultrasmall gold course went well.
Rosemary


.At 02:38 PM 7/10/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jul 10 19:17:29 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu (by way of
Date: Wed, 10 Jul 2002 17:02:27 -0700
Subject: Haskris Water Chiller

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Haskris Water to Air Chiller, puchased in 1994 to cool one Jeol 840 SEM and
a Jeol 100C TEM, from 1994 to 1996. 1996 to present only used for the TEM.
Unit is in very good condition. Asking $20000.00 or Best offer plus
removal and shipping costs. Contact

Joe Goodhouse
Confocal / EM Core Laboratory Manager
Department of Molecular Biology
Princeton University
609-258-5432
jgoodhouse-at-molbio.princeton.edu

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html


From daemon Wed Jul 10 23:26:26 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Wed, 10 Jul 2002 21:15:58 -0400
Subject: Re: Stop algae growth (plus pH control)

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on 7/9/02 7:37 PM, Allan Mitchell at allan.mitchell-at-stonebow.otago.ac.nz
wrote:

}
} Hi Wilbur, Thanks for the update
}
} The recirculating cooling water systems problem that we have the most
} problem trying to get a handle on is controlling the pH of the
} cooling water to prevent corrosion. Since the end of the good old
} days when we were allowed to use Sodium Chromate and Sodium
} Bicarbonate we have not yet found a satisfactory replacement.
}
} Satisfactory includes; effectiveness at pH control, life time before
} depletion, safe handling and disposal, cost, availablility.
}
} Trying to get good advice also seems to like the proverbial hens teeth.
}
} Anyone out there come up with any good products recently.
}
Dear Allan,
Back at the HVEM, we used Aqua Treet 42 for anti-corrosion and adjusted
the pH to between 8.0 and 8.5 with NaOH. The Aqua Treet--from Aqua Labs
somewhere in NJ as I recall--works well at that pH. We checked the
concentration with a color kit once each month, and had to add more only
occasionally; it is very safe (I didn't drink any, but it is not corrosive,
etc.); the cost was under $100 for a 5 gal tub, which has lasted for many
years, and is nowhere near the bottom. Last time I checked, Aqua Labs was
reachable by phone and was on the web. Good luck.
Yours,
Bill Tivol



From daemon Wed Jul 10 23:27:38 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Wed, 10 Jul 2002 21:19:36 -0400
Subject: Re: SEM&LM - help needed for Metal localization

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on 7/9/02 7:46 PM, rcmoretz-at-att.net at rcmoretz-at-att.net wrote:

} Altho' not directly involved in the
} work, I do remember that the fixative was hydrogen
} sulfide saturated glutaraldehyde (really noxious mixture-
} -in more ways than one!). There is also some literature
} related to Cd localization, but I can't dredge up
} names.
} Roger Moretz
} --
} Where the world is only slightly
} less weird than it actually is.

Dear Roger,
Sulfide should precipitate Cd as well as Zn.
Yours,
Bill Tivol



From daemon Thu Jul 11 06:23:28 2002



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Thu, 11 Jul 2002 13:13:38 +0200
Subject: Re: SEM: Removing cell membranes

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Randy
There was a presentation at a South African EM Society meeting in 1983
on this subject:
Hughes, F & Rijkenberg, F H J - 1983: An epidermis removal technique
for studying infection processes of Puccinia sorghi in Maize leaves.
Proc. Electron Microsc. Soc. South Afr. 13, 17-18.

The basics are: Fix and dehydrate specimens, then CPD, mount onto SEM
stub. A second stub, onto which double-sided sticky tape has been
affixed, is pressed to the specimen surface and pulled away. After
coating both stubs, the inner and outer surfaces of the specimen can
be studied.

If required, I can fax you a copy of the two pages.

Regards
Jan Coetzee


"Tindall, Randy D." wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear listers,
}
} I seem to recall reading somewhere about a method of removing the top cell membranes from a cell culture layer. It seems to have involved placing a layer of material over the cultured cells and stripping it away, but I can't remember anything about where I saw the reference.
}
} Can somebody help jog my memory? Or did I dream this? I figure if ANYONE knows, you folks will!
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/

--
Jan Coetzee
Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/academic/electron/emunit1.htm


From daemon Thu Jul 11 09:11:48 2002



From: cwuethri-at-caregroup.harvard.edu
Date: Thu, 11 Jul 2002 09:59:39 -0400
Subject: Re: Congo red

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Hi Mark,

Some weeks ago, there was a discussion about the effects of pH and many
other factors on the stainings (in that case toluidine blue). The conclusion
was, if I can say, that we can never know ...., only try.

Regarding your problem, I had a similar experiment with ruthenium red on JB4
embedded European beeches sections. At neutral and basic pH, the stain is in
the cytoplasm (or in the vacuoles?).
At pH {= 5, the pectins (near the walls) stained. It remained me an
experiment I did on practical work (when I was student) in which we
experimented that the staining of the walls migrates to the vacuols when the
pH is changed from acidic to basic...

You can try quickly the same with your sections:

1) stain them as you do
2) rince them with an acidic buffer or solution and mount them in that
buffer (non permanent mounting)

3) look at them, perhaps you will see after some time that the staining in,
or near the walls

4) if it works, one conclusion would be : the pH of rinsing is as (or
perhaps in some cases) more important than the pH of the staining itself.
But I stop here, because I'm sure there will be many other opinions...

Another problem could perhaps be that embedding was not optimal (did you
your staining on the same sections than when it worked ?)...

Hope it hels

Chris Wuethrich
Beth Israel Deaconess medical Center
330, Brookline AVE
Boston, MA 02215


From daemon Thu Jul 11 09:11:48 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 11 Jul 2002 10:02:59 -0400
Subject: RE: ???TEM, Epon sections, problem with "pepper"

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PCan someone please explain why the solutions still concentrate on the stain
and its condition rather than taking the tack that the problem lies with the
Philips scope?

Fred Monson

} ----------
} From: Monson, Frederick C.
} Sent: Wednesday, July 10, 2002 11:28 AM
} To: 'Stefan Geimer'
} Cc: 'List-Microscopy'
} Subject: RE: TEM, Epon sections, problem with "pepper"
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} But do you have the problem with a section that is taken from the Philips
} to
} the Zeiss?
}
} Thanks,
}
} Fred Monson
}
} } ----------
} } From: Stefan Geimer
} } Sent: Tuesday, July 9, 2002 2:04 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: TEM, Epon sections, problem with "pepper"
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I have the following problem:
} } I am observing Epon sections, stained with uranyl acetate and lead
} } citrate in an Philips EM 201. The sections show a really severe
} } contamination with electron dense grains. These grains are always
} } associated with embedded structures (membranes, microtubules etc.).The
} } problem started after I changed the cathode. The contamination looks
} } kinda like lead-stain granularity and I think it has something to do
} } with the lead. A section stained with uranyl acetate only looks fine
} } (but I need the lead to get enough stain).
} } The crazy thing is that I don´t have the problem when I use our other
} } scope, a Zeiss EM 10. So if I take one of my sections and have a look at
} } it in the Zeiss EM 10, everything is perfect. The same section observed
} } in the Philips EM 201 shows this contamination with electron dense
} } grains. Both scopes operated at comparable conditions (80 kv, cold trap
} } etc.). So the contamination seems to be lead citrate and scope
} } dependend.
} } Anybody ever had that problem and might have an idea how to solve it?
} }
} } Stefan
} }
} }
} }
} } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} } Stefan Geimer
} } MCDB Dept.
} } Yale University
} } P.O. Box 208103
} } New Haven, CT 06520-8103
} } U.S.A.
} }
} } Tel.: 203/432-3473
} } Fax.: 203/432-6210
} }
} } e-mail: stefan.geimer-at-yale.edu
} } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} }
} }
} }
} }
}
}


From daemon Thu Jul 11 09:31:44 2002



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 11 Jul 2002 10:25:35 -0600
Subject: Re: SEM: Removing cell membranes

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Check the work of Richard G Anderson (Department of Cell Biology,
University of Texas Southwestern Medical Center, Dallas).

One example of publication which I think described that technique :
Moore MS, Mahaffey DT, Brodsky FM, Anderson RG.
Assembly of clathrin-coated pits onto purified plasma membranes.
Science 1987 May 1;236(4801):558-63.

"Tindall, Randy D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear listers,
}
} I seem to recall reading somewhere about a method of removing the top cell membranes from a cell culture layer. It seems to have involved placing a layer of material over the cultured cells and stripping it away, but I can't remember anything about where I saw the reference.
}
} Can somebody help jog my memory? Or did I dream this? I figure if ANYONE knows, you folks will!
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/

--
Marc Pypaert
Department of Cell Biology,
Center for Cell and Molecular Imaging and
Ludwig Institute for Cancer Research,
Yale University School of Medicine
333 Cedar Street
New Haven CT 06520
Tel : (203) 785 3681
Fax : (203) 785 7446




From daemon Thu Jul 11 10:59:10 2002



From: Xinran Liu :      xinran.liu-at-utsouthwestern.edu
Date: Thu, 11 Jul 2002 10:53:38 -0500
Subject: TEM film scanner

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Dear Colleagues,

I plan to upgrade my film scanner for TEM 3.25x4 in. negatives.
(Currently I am using Microtek ScanMaker 8700 and Agfa Duoscan T2500). I
have located two scanners that fit into my requirement. One is Minolta
Dimage Scan multi Pro, the other one is Nikon Super Coolscan 8000ED.
Minolta scanner has a slightly higher optical resolution (4800 dpi) and
Dynamic range of 4.8.

Can anybody who has experience with these scanners help me to make a
decision? Thanks a lot.

Xinran Liu

Xinran Liu, M.D., Ph.D.
Center for Basic Neuroscience
UT Southwestern Medical Center at Dallas
Phone: 214-648-1830
Fax: 214-648-1801
E-mail: xinran.liu-at-utsouthwestern.edu






From daemon Thu Jul 11 11:32:57 2002



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Thu, 11 Jul 2002 11:30:05 -0500
Subject: RE: ???TEM, Epon sections, problem with "pepper"

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The reason why I took this position is because I have sometimes seen
peppering as a result of faulty staining technique with lead citrate. I
have not see this sort of "peppering" as a result of microscope
contamination. In the case of microscope contamination, it shows more as a
general density increase over a specific area of the grid....like a burn.

That said, I've never used a Philips EM, but I have used Jeol 1010, Hitachi
7000, AEI 6B and AEI 801, Zeiss 109, and a Zeiss 10CR and an old Zeiss 9 I
believe. In my 18 years of experience, I've never seen peppering as a
result of a microscope contamination. It doesn't mean that it can't happen,
just that in my experience, I haven't seen it. I can only talk about what
I've seen, or haven't seen.


PCan someone please explain why the solutions still concentrate on the stain
and its condition rather than taking the tack that the problem lies with the
Philips scope?

Fred Monson

} ----------
} From: Monson, Frederick C.
} Sent: Wednesday, July 10, 2002 11:28 AM
} To: 'Stefan Geimer'
} Cc: 'List-Microscopy'
} Subject: RE: TEM, Epon sections, problem with "pepper"
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} But do you have the problem with a section that is taken from the Philips
} to
} the Zeiss?
}
} Thanks,
}
} Fred Monson
}
} } ----------
} } From: Stefan Geimer
} } Sent: Tuesday, July 9, 2002 2:04 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: TEM, Epon sections, problem with "pepper"
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I have the following problem:
} } I am observing Epon sections, stained with uranyl acetate and lead
} } citrate in an Philips EM 201. The sections show a really severe
} } contamination with electron dense grains. These grains are always
} } associated with embedded structures (membranes, microtubules etc.).The
} } problem started after I changed the cathode. The contamination looks
} } kinda like lead-stain granularity and I think it has something to do
} } with the lead. A section stained with uranyl acetate only looks fine
} } (but I need the lead to get enough stain).
} } The crazy thing is that I don´t have the problem when I use our other
} } scope, a Zeiss EM 10. So if I take one of my sections and have a look at
} } it in the Zeiss EM 10, everything is perfect. The same section observed
} } in the Philips EM 201 shows this contamination with electron dense
} } grains. Both scopes operated at comparable conditions (80 kv, cold trap
} } etc.). So the contamination seems to be lead citrate and scope
} } dependend.
} } Anybody ever had that problem and might have an idea how to solve it?
} }
} } Stefan
} }
} }
} }
} } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} } Stefan Geimer
} } MCDB Dept.
} } Yale University
} } P.O. Box 208103
} } New Haven, CT 06520-8103
} } U.S.A.
} }
} } Tel.: 203/432-3473
} } Fax.: 203/432-6210
} }
} } e-mail: stefan.geimer-at-yale.edu
} } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} }
} }
} }
} }
}
}



From daemon Thu Jul 11 11:42:30 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Thu, 11 Jul 2002 12:32:17 -0400
Subject: Haskris Correction

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Haskris Water to Air Chiller, purchased in 1994 to cool one Jeol 840 SEM and
a Jeol 100C TEM, from 1994 to 1996. 1996 to present only used for the TEM.
This is a Model R150 Unit. 208/230 volts , 1 Phase 60 Hertz. Condenser is
1.75 HP. with lbs. refrigerant R-22 charge ,1/3 HP water pump with a 14
gallon tank. Unit is in very good condition. Asking $2,000.00 or Best offer
plus removal and shipping costs. Contact


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432
jgoodhouse-at-molbio.princeton.edu

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Thu Jul 11 13:21:11 2002



From: agodl-at-o2.pl (by way of MicroscopyListserver)
Date: Thu, 11 Jul 2002 11:08:34 -0700
Subject: Ask-A-Microscopist:Timm Method??

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (agodl-at-o2.pl) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
July 11, 2002 at 11:30:16
---------------------------------------------------------------------------

Email: agodl-at-o2.pl
Name: Godlewski Andrzej

Education: Graduate College

Location: Medical University of Lodz, Lodz, Poland

Question: Hallo,
In the autometallography the Timm method is used for revealing
cations (eg zinc, cobalt)in different biological materials. The idea
of This method is simply from chemical point of view: treatment of
sample with Na2S (high pH)[sulphide of cation present in sample],
wash, next AgNO3 in H2O (1%?)[substitution by silver other cattion ]
and photographic developer [for silver revealing].The method is
simply, accurate but not specific. The question: What is original
recipe of Timm method?
Best regard
A.Godlewski MD PhD D Sci

---------------------------------------------------------------------------


From daemon Thu Jul 11 14:33:21 2002



From: Paul Toselli :      paultos-at-biochem.bumc.bu.edu
Date: Thu, 11 Jul 2002 15:29:07 -0400
Subject: How to prevent mouse-brain paraffin-section loss

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Hello,

Our 5-micron paraffin-embedded mouse brain secions are washing off
our plus-coated microsope slides during our post-xylene alcohol
dehydration steps.

Does any one know how to prevent this from occuring?

Thank you.

Paul Toselli
Boston University Medical School




From daemon Thu Jul 11 14:44:54 2002



From: Paul Toselli :      paultos-at-biochem.bumc.bu.edu
Date: Thu, 11 Jul 2002 15:42:23 -0400
Subject: How to prevent mouse-brain paraffin-section loss

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Our 5-micron paraffin-embedded mouse brain secions are washing off
our plus-coated microsope slides during our post-xylene alcohol
dehydration steps.

Does any one know how to prevent this from occuring?

Thank you.

Paul Toselli
Boston University Medical School






From daemon Thu Jul 11 16:51:48 2002



From: Jian-Guo Zheng :      j-zheng3-at-northwestern.edu
Date: Thu, 11 Jul 2002 16:42:17 -0500
Subject: Electron Microscopist: position open

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Electron Microscopist
Electron Probe Instrumentation Center (EPIC)
Northwestern University, USA

Job description:

Research, collaboration and training of students and users of EPIC in
all aspects of electron microscopy: particularly specimen preparation
and FIB, SEM and TEM analysis.

Specific duties include:

(1) Teach, help and actively collaborate with users in preparing TEM/SEM
samples and their observation by SEM/TEM.
(2) Assist and conduct laboratory teaching in UG and graduate courses
related to specimen preparation and electron microscopy.
(3) Develop collaborative and independent research projects and topics
related to advanced electron microscopy and nanostructures.
(4) Maintain and develop sample preparation laboratory and equipment
such as FIB, ultramicrotome, IBTs, PIPS, electrojet polishers, cutting
saw, wire saw, grinder, vacuum evaporator, sputter coater, liquid
nitrogen and other accessories.
(5) Help maintain and develop computer facility within EPIC.

Qualifications and Needs:

A technical degree in physical science/engineering or bioscience is
needed. Actual hands-on experience in specimen preparation of hard and
soft materials, and electron microscopy (SEM/TEM) is required. Need to
be familiar with modern computers and basic user programs. Laboratory
teaching experience is highly desirable.

Excellent prospects for personal and professional growth.

Send Resume and 3 References directly to:

Prof. Vinayak P. Dravid
Materials Science & Engineering
Director, Electron Probe Instrumentation Center (EPIC)
2225 N. Campus Drive, 1133 MLSF
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 467-6573
E-mail: v-dravid-at-northwestern.edu
http://vpd.ms.northwestern.edu
http://epic.ms.northwestern.edu
**********************************************************




From daemon Thu Jul 11 17:03:03 2002



From: Jian-Guo Zheng :      j-zheng3-at-northwestern.edu
Date: Thu, 11 Jul 2002 16:55:58 -0500
Subject: Electron Microscopist: Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Electron Microscopist
Electron Probe Instrumentation Center (EPIC)
Northwestern University, USA

Job description:

Research, collaboration and training of students and users of EPIC in
all aspects of electron microscopy: particularly specimen preparation
and FIB, SEM and TEM analysis.

Specific duties include:

(1) Teach, help and actively collaborate with users in preparing TEM/SEM
samples and their observation by SEM/TEM.
(2) Assist and conduct laboratory teaching in UG and graduate courses
related to specimen preparation and electron microscopy.
(3) Develop collaborative and independent research projects and topics
related to advanced electron microscopy and nanostructures.
(4) Maintain and develop sample preparation laboratory and equipment
such as FIB, ultramicrotome, IBTs, PIPS, electrojet polishers, cutting
saw, wire saw, grinder, vacuum evaporator, sputter coater, liquid
nitrogen and other accessories.
(5) Help maintain and develop computer facility within EPIC.

Qualifications and Needs:

A technical degree in physical science/engineering or bioscience is
needed. Actual hands-on experience in specimen preparation of hard and
soft materials, and electron microscopy (SEM/TEM) is required. Need to
be familiar with modern computers and basic user programs. Laboratory
teaching experience is highly desirable.

Excellent prospects for personal and professional growth.

Send Resume and 3 References directly to:

Prof. Vinayak P. Dravid
Materials Science & Engineering
Director, Electron Probe Instrumentation Center (EPIC)
2225 N. Campus Drive, 1133 MLSF
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 467-6573
E-mail: v-dravid-at-northwestern.edu
http://vpd.ms.northwestern.edu
http://epic.ms.northwestern.edu
**********************************************************



From daemon Thu Jul 11 18:10:14 2002



From: Carlos Kazuo Inoki :      kazuo-at-csc.albany.edu
Date: Thu, 11 Jul 2002 19:02:27 -0400 (EDT)
Subject: Re: TEM film scanner

Contents Retrieved from Microscopy Listserver Archives
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Hi,

This is my experience with Minolta Scan Multi Pro film scanner.
After a couple of months using it I had to adapt the way I take my TEM
pictures to the scanner. The reason is that the 8x10cm negatives can't be
rotated even using the multi format film holder. And the largest area it
can scan is something around 6x9cm. If you are scanning a small area in
the negative this in not a problem. But in my case some of the pictures I
take is at low mag (less than 5k), and cover almost the entire negative.
What I do is choose a magnification that can fit everything of interest in
the "scannable" area. About the dynamical range, I still don't see much
difference between a negative scanned using the Minolta film scanner or
the old Epson tabletop scanner we have (supposed to be 3.0). But maybe it
is just a matter of getting used to the new scanner. BTW, if you download
the manual of this scanner from Minolta, you will notice in the
Specification section a notice about the Dynamical Range, "4.2 (tested
value)". Maybe this 4.8 is obtained under some "special" condition. :P

The Nikon one should not be much different, I suppose. :)

Regards,

Carlos

On Thu, 11 Jul 2002, Xinran Liu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
}
} I plan to upgrade my film scanner for TEM 3.25x4 in. negatives.
} (Currently I am using Microtek ScanMaker 8700 and Agfa Duoscan T2500). I
} have located two scanners that fit into my requirement. One is Minolta
} Dimage Scan multi Pro, the other one is Nikon Super Coolscan 8000ED.
} Minolta scanner has a slightly higher optical resolution (4800 dpi) and
} Dynamic range of 4.8.
}
} Can anybody who has experience with these scanners help me to make a
} decision? Thanks a lot.
}
} Xinran Liu

o-------------------------------------------------------o
| Carlos Kazuo Inoki |
| Department of Physics - University at Albany |
| 1400 Washington Ave.- Albany - NY - 12222 |
o-------------------------------------------------------o



From daemon Thu Jul 11 21:56:35 2002



From: Microscopy Today :      microtoday-at-attglobal.net
Date: Thu, 11 Jul 2002 21:44:46 -0500
Subject: FW: Microscopy Today July/August Issue TofC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Reproduced here is the table of contents for the July/August issue of
Microscopy Today.



It is in production at this time and should mail by the 12th.


Ron Anderson

Editor, Microscopy Today



MT JULY/AUGUST TofC



Very Tiny Bar Codes, Stephen W. Carmichael, Mayo Clinic



Progress Towards More Realistic In-Situ Microscopy Observations, A.
Howie, Cavendish Laboratory, University of Cambridge



Spectral Image Analysis: Getting The Most From All That Data, P.G.
Kotula, M.R. Keenan, and R. Loehman, Sandia National Laboratories



New Fluorescent Labeling Technologies for Ultrasensitive Cytochemical
and Histochemical Imaging, Iain Johnson, Molecular Probes, Inc



Artifacts and Non-Local Effects In SPM Potential Measurements, Sergei
V. Kalinin and Dawn A. Bonnell, University of Pennsylvania



Vibration, Resonance and the Effect on Microscopes , Douglas A.
Anderson, Schnabel Engineering Associates



The Basics of Immunoglobulins and Immunostaining, W. Gray (Jay)
Jerome, Vanderbilt University Medical Center



Swapping Atoms For Bits: Managing The Digital Evolution In The
Microscopy Laboratory, Judy A. Murphy, San Joaquin Delta College



A Note on Iodine and Vacuum , Scott D. Walck, PPG Industries, Inc.



A Simple Way to Eliminate Frost Build-up on Cryo-SEM Samples, Gib
Ahlstrand, University of Minnesota



Mousescope, Lee van Hook, Piltdown Research Institute, Münchhausen University



A Method for Safely Restraining Mouse Pups for Microscopy, Michael J.
Herron, University of Minnesota



A Simple Image Archive That's Cheap, Too! , Tim Morken, Centers for
Disease Control and Prevention




From daemon Fri Jul 12 01:15:40 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 11 Jul 2002 23:06:52 -0700
Subject: RE: ???TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
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I could not imagine the situation when the SAME section will looks dirty in
one microscope (does not matter Phillips or not) and perfectly fine in
another... Only one explanation: the 'good' microscope is misaligned
(sorry EM10) in such degree that you do see practically nothing (the dirty
things becomes invisible). I am so sorry for such rude interpretation. I
would more believe to the 'worse' microscope and will pay attention to the
staining procedure to avoid precipitates.

Sergey

At 09:30 AM 7/11/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Jul 12 06:57:07 2002



From: kivambe :      kivambe-at-uccmail.co.tz
Date: Fri, 12 Jul 2002 14:38:35 +0300 (EAT)
Subject: TEM/EDS of wood Question.

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

How do I prepare bamboo wood for TEM (Microstructure) and EDS?
please, kindly give ideas,suggestions.

Thanks,

Maulid M. Kivambe
Electron Microscopy Laboratory,
Faculty of Science,
P.O.Box 35065,
Dar es Salaam,
Tanzania.

Phone: 255 022 2410462
Mobile: 255 0744 266667
Fax 255 022 2410480.



From daemon Fri Jul 12 06:57:07 2002



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 12 Jul 2002 04:36:16 -0700 (PDT)
Subject: RE: ???TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
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Dear Fred and those interested in reading...

I used a Philips 201 for a number of years and got great results as long
as I did not dwell too long in a particular area or a single grid.
However, I always used LN2 whenever I examined or photographed samples
because of the issue associated with vacuum contamination. You could
place a specimen in the field of view and literally watch the
contamination particles form!

The 201 had a mercury/oil diffusion pump and although this may not have
been the entire issue, it was a problem. The other issue was the age of
the instrument. My question would be: how many filament hours are you
getting out of the 201? This is frequently a choice method for
determining scope operation and vacuum function.

I have used and am still using a Zeiss EM10CA since purchased new in 1986.
In addition to the oil diffusion pump, the scope has twin getter pumps: I
always use LN2 when viewing or imaging specimens. I can sit there for 15
minutes and never see contamination. This is true for tissue embedded in
LR White, Epon, Epon substitutes, Spurrs, etc. Furthermore, I get an
average of 300 hours on Zeiss supplied tungsten filaments: yes, this is
true!!

So, Fred, I believe you are correct in your deduction!

Sincerely,
Ken

------
Ken Tiekotter,
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203 USA

Director, MicroImaging Center, G50
Legacy Portland Hosptials
Legacy Holladay Park Medical Center
1225 NE 2nd Avenue
Portland, OR 97232 USA

Tel.: 503-413-5391

On Thu, 11 Jul 2002, Monson, Frederick C. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} PCan someone please explain why the solutions still concentrate on the stain
} and its condition rather than taking the tack that the problem lies with the
} Philips scope?
}
} Fred Monson
}
} } ----------
} } From: Monson, Frederick C.
} } Sent: Wednesday, July 10, 2002 11:28 AM
} } To: 'Stefan Geimer'
} } Cc: 'List-Microscopy'
} } Subject: RE: TEM, Epon sections, problem with "pepper"
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } But do you have the problem with a section that is taken from the Philips
} } to
} } the Zeiss?
} }
} } Thanks,
} }
} } Fred Monson
} }
} } } ----------
} } } From: Stefan Geimer
} } } Sent: Tuesday, July 9, 2002 2:04 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: TEM, Epon sections, problem with "pepper"
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } I have the following problem:
} } } I am observing Epon sections, stained with uranyl acetate and lead
} } } citrate in an Philips EM 201. The sections show a really severe
} } } contamination with electron dense grains. These grains are always
} } } associated with embedded structures (membranes, microtubules etc.).The
} } } problem started after I changed the cathode. The contamination looks
} } } kinda like lead-stain granularity and I think it has something to do
} } } with the lead. A section stained with uranyl acetate only looks fine
} } } (but I need the lead to get enough stain).
} } } The crazy thing is that I don´t have the problem when I use our other
} } } scope, a Zeiss EM 10. So if I take one of my sections and have a look at
} } } it in the Zeiss EM 10, everything is perfect. The same section observed
} } } in the Philips EM 201 shows this contamination with electron dense
} } } grains. Both scopes operated at comparable conditions (80 kv, cold trap
} } } etc.). So the contamination seems to be lead citrate and scope
} } } dependend.
} } } Anybody ever had that problem and might have an idea how to solve it?
} } }
} } } Stefan
} } }
} } }
} } }
} } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} } } Stefan Geimer
} } } MCDB Dept.
} } } Yale University
} } } P.O. Box 208103
} } } New Haven, CT 06520-8103
} } } U.S.A.
} } }
} } } Tel.: 203/432-3473
} } } Fax.: 203/432-6210
} } }
} } } e-mail: stefan.geimer-at-yale.edu
} } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} } }
} } }
} } }
} } }
} }
} }
}




From daemon Fri Jul 12 07:41:16 2002



From: Gunnar Glasser :      glasser-at-mpip-mainz.mpg.de
Date: Fri, 12 Jul 2002 14:40:13 +0200
Subject: SEM Leo Gemini 15xx / Zeiss DSM982 "improved Inlens detector"

Contents Retrieved from Microscopy Listserver Archives
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Hi,

i wonder if there are people out there who already possess the improved Inlens
detector for the LEO Gemini 15xx or the Zeiss DSM 982.

According to their sales people, the detection efficiency was increased by 250 .. 300%
and the new the detector material is supposed to be "radian hard" ... so far that is
what they told us - Sounds to good to be true?

We would like to get hold of comparable user-image material which shows the same
sample observed a)with your old and b)with the improved Inlens detector.
(... parameter's like acquisition time, detector settings and so on should be provided if
possible)

"Any" thougths, suggestions and experiences are welcome!

Gunnar



Dipl.Ing.(FH) G.Glasser, Elektronenmikroskopie
Max Planck Institut fuer Polymerforschung
Ackermannweg 10
55128 Mainz, GERMANY
phone:++49 +6131 379195 379391
fax: ++49 +6131 379100
web: http://www.mpip-mainz.mpg.de/~glasser

Disclaimer:
The statement above is mine alone and does not implicitly represent the position
of the MPI-P or the MPG!



From daemon Fri Jul 12 10:35:22 2002



From: Barbara Maloney :      bmalon01-at-fiu.edu
Date: Fri, 12 Jul 2002 11:20:10 -0400
Subject: TEM methods for nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Group - what would be the best procedure to prep a copper TEM grid
with nanoparticles? What glue or adhesive would you suggest? I'm
actually using a STEM holder (to get TEM image) on a SEM.
Thanks
Barb



From daemon Fri Jul 12 10:44:40 2002



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Fri, 12 Jul 2002 11:37:29 -0400
Subject: LM, Workshop on "Use of The Microscope", NYMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


New York Microscopical Society
30 North Mountain Avenue
Montclair, NJ 07042


Bernard Friedman
Memorial Workshop



Use of the Microscope
September 21, 28, October 5, 12, 2002

A basic course on light microscopy which will cover the following topics:
Theory of microscopy
Kohler Illumination
Diffraction Theory
Contrast Methods
Polarized light
Phase Contrast
Interference
. . . . Hoffman contrast, Rheinberg, Dark-field & oblique Illumination,
etc.

The workshop will consist of four consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of microscopy.
The course instructors include Jan Hinsch of Leica, Inc., Dennis O’Leary of
Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of SensIR
Technologies, Inc. and N.Y.M.S. Instructor Don O'Leary.

WHEN: September 21, 28, October 5, 12, 2002 from 10 A.M. to 4 P.M.

WHERE: 30 N. Mountain Avenue, Montclair, NJ, Phone (973) 744-0043 (
parking available, accessible by public transportation. Information on car
pools and transportation will be provided.)

COST: $325 for N.Y.M.S. members, $355 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: Beginners and experienced users who wish to learn more about the
proper use of a microscope.

HOW: Register using the form below. Limited to the first 12 registrants.
Send form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849
E-mail donoleary-at-worldnet.att.net Fax (425) 988-1415

PLEASE POST
----------------------------------------------------------------------------
-------------------
Registration Form
Use of the Microscope 2002

N.Y.M.S. Member_________________ ($275) Non-Member__________($295)

Name______________________________________________________________________
Address____________________________________________________________________
Phone (W)_______________________(H)___________________________




From daemon Fri Jul 12 11:39:31 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 12 Jul 2002 12:29:08 -0400
Subject: RE: ???TEM, Epon sections, problem with "pepper"

Contents Retrieved from Microscopy Listserver Archives
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Timing is sometimes NOT everything, BUT in this case it was a fault. My
apology for the timing of my general question which was directed generally
BUT in time appeared as a direct response to Garry's suggestion.

In any case, for those who address my question, including Garry, I
appreciate the discussion and the enlightenment from those who have spent
much more time in the driver's seat than I.

I often pose questions in a way that can cause others to bristle, but my
questions are only intended to seek an answer. You see, I'm not certain
what 'peppering' even refers to when one is discussing artifact on a 60-90nm
section. I know that if I have such a problem a year from now that I will
take the grid to the SEM to try to determine whether the contamination is in
or on the section. On the other hand, if nothing else counts in this
business, experience does, and I listen most intently to those with
experience when they speak.

Respectfully yours,

Fred Monson

} ----------
} From: Garry Burgess
} Sent: Thursday, July 11, 2002 12:30 PM
} To: 'Monson, Frederick C.'; 'List-Microscopy'
} Subject: RE: ???TEM, Epon sections, problem with "pepper"
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The reason why I took this position is because I have sometimes seen
} peppering as a result of faulty staining technique with lead citrate. I
} have not see this sort of "peppering" as a result of microscope
} contamination. In the case of microscope contamination, it shows more as
} a
} general density increase over a specific area of the grid....like a burn.
}
} That said, I've never used a Philips EM, but I have used Jeol 1010,
} Hitachi
} 7000, AEI 6B and AEI 801, Zeiss 109, and a Zeiss 10CR and an old Zeiss 9 I
} believe. In my 18 years of experience, I've never seen peppering as a
} result of a microscope contamination. It doesn't mean that it can't
} happen,
} just that in my experience, I haven't seen it. I can only talk about what
} I've seen, or haven't seen.
}
}
} PCan someone please explain why the solutions still concentrate on the
} stain
} and its condition rather than taking the tack that the problem lies with
} the
} Philips scope?
}
} Fred Monson
}
} } ----------
} } From: Monson, Frederick C.
} } Sent: Wednesday, July 10, 2002 11:28 AM
} } To: 'Stefan Geimer'
} } Cc: 'List-Microscopy'
} } Subject: RE: TEM, Epon sections, problem with "pepper"
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } But do you have the problem with a section that is taken from the
} Philips
} } to
} } the Zeiss?
} }
} } Thanks,
} }
} } Fred Monson
} }
} } } ----------
} } } From: Stefan Geimer
} } } Sent: Tuesday, July 9, 2002 2:04 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: TEM, Epon sections, problem with "pepper"
} } }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } I have the following problem:
} } } I am observing Epon sections, stained with uranyl acetate and lead
} } } citrate in an Philips EM 201. The sections show a really severe
} } } contamination with electron dense grains. These grains are always
} } } associated with embedded structures (membranes, microtubules etc.).The
} } } problem started after I changed the cathode. The contamination looks
} } } kinda like lead-stain granularity and I think it has something to do
} } } with the lead. A section stained with uranyl acetate only looks fine
} } } (but I need the lead to get enough stain).
} } } The crazy thing is that I don´t have the problem when I use our other
} } } scope, a Zeiss EM 10. So if I take one of my sections and have a look
} at
} } } it in the Zeiss EM 10, everything is perfect. The same section
} observed
} } } in the Philips EM 201 shows this contamination with electron dense
} } } grains. Both scopes operated at comparable conditions (80 kv, cold
} trap
} } } etc.). So the contamination seems to be lead citrate and scope
} } } dependend.
} } } Anybody ever had that problem and might have an idea how to solve it?
} } }
} } } Stefan
} } }
} } }
} } }
} } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} } } Stefan Geimer
} } } MCDB Dept.
} } } Yale University
} } } P.O. Box 208103
} } } New Haven, CT 06520-8103
} } } U.S.A.
} } }
} } } Tel.: 203/432-3473
} } } Fax.: 203/432-6210
} } }
} } } e-mail: stefan.geimer-at-yale.edu
} } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
} } }
} } }
} } }
} } }
} }
} }
}
}
}


From daemon Fri Jul 12 11:44:09 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 12 Jul 2002 17:41:33 +0100 (GMT Daylight Time)
Subject: Re: TEM methods for nanoparticles

Contents Retrieved from Microscopy Listserver Archives
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Hi Barbara,

Assuming you really do mean nano particles and not micron
sized then just drop them onto a carbon support filmed Cu
grid and they will usually stick. Larger particles will
tend to come off or charge and fly off but most nano and
10s of nanometre particles will be OK. Of course if they
are magnetic the field might strip them off and if they are
non conductive charging will be worse etc.

The alternative is to suspend them (in ethanol) and drop a
drop of it onto the carbon support grid resting on a filter
paper. They will also usually stick but beware of
contaminating the surface by using dirty pipette, alcohol
etc. Nothing should be stored in plastic and wash the
utensils before use then you should be OK.

Good luck,
Ron


On Fri, 12 Jul 2002 11:20:10 -0400 Barbara Maloney
{bmalon01-at-fiu.edu} wrote:

} ------------------------------------------------------------------------
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}
}
} Dear Group - what would be the best procedure to prep a copper TEM grid
} with nanoparticles? What glue or adhesive would you suggest? I'm
} actually using a STEM holder (to get TEM image) on a SEM.
} Thanks
} Barb
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri Jul 12 11:51:36 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 12 Jul 2002 11:44:08 -0500
Subject: Re: Removing Cell Membranes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many thanks to all who replied to my question about removing cell membranes for SEM! Lots of leads, references, and a couple complete protocols to keep me busy for awhile.

What a great list!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Fri Jul 12 13:39:50 2002



From: Mark Riggs :      Mark.Riggs-at-asml.com
Date: Fri, 12 Jul 2002 14:30:44 -0400
Subject: xl40feg imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


primary application is imaging of Pt coated (2-3nm) resist lines of 100nm and less. standard scope parameters under 100,000x have been 10kv spot2. now find that i must run at 20-30kv to image clearly, and image degrades over time (as little as ½ hour). FEI boosted µA to 334 and performed alignments. what amazes me is that i am no longer damaging my samples. its as if the beam lacks the "oomph" to bend over the lines like it used to at even 10kv. is this a "beam density" issue?

Mark Riggs
SEM Metrology
ASML, Wilton, CT
mark.riggs-at-asml.com
ph:203-761-6856
lab:203-761-4403





From daemon Fri Jul 12 14:13:36 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Fri, 12 Jul 2002 15:02:35 -0400
Subject: TMC Anti-vibration Platform

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a TMC MICRO-g Vibration Isolation System Model # 65-17171-01
that we wish to sell. The platform is 86" wide by 71" deep with a seating
cut out that is -at- 30" wide narrowing down to 12" and is about 38" deep.
System has 4 isolation feet. With the feet, it requires an area that is 97"
x 82". The weight of unit is between 2200 - 2500 lbs. Asking $4,000.00 or
best offer plus shipping and handling charges. Interested parties should
contact


Joe Goodhouse
Confocal / EM Core Laboratory Manager
Department of Molecular Biology
Princeton University
609-258-5432
jgoodhouse-at-molbio.princeton.edu

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Fri Jul 12 14:19:42 2002



From: mdhaque :      mdhaque-at-students.uiuc.edu
Date: Fri, 12 Jul 2002 14:13:10 -0500
Subject: TEM Dislocations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have a question: With TEM how can I be sure that the feature I am looking at
is/are dislocation(s)? Can you suggest any reading on dislocation
imaging..Thanks

Aman Haque

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Aman Haque
Dept of Mechanical & Industrial Engg
University of Illinois at Urbana Champaign

Tel: 217-244-2760, Fax: 217-244-6534



From daemon Fri Jul 12 15:47:22 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 12 Jul 2002 16:37:46 -0400
Subject: FEI Quanta?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dopes anyone have a FEI Quanta working that s/he would talk to me about on
the QT?

Sure would appreciate the info.

Thanks all and have a nice weekend,

Fred

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


From daemon Fri Jul 12 18:15:35 2002



From: Dwight Arrieche (IIBCA) :      darriech-at-cumana.sucre.udo.edu.ve
Date: Fri, 12 Jul 2002 18:59:55 -0400 (VET)
Subject: On line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Greatly appreciate send me Listserver communications. Account were bloked
for a couple of weeks.

Thanks



From daemon Fri Jul 12 18:15:50 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 12 Jul 2002 17:17:32 -0600
Subject: TEM Dislocations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dislocations have very specific visibility criteria in a TEM, which are
basically determined by their burgers vector. I am not close to my TEM books
right now, so I can't be more specific, but I am sure there are other people
here who are very familiar with dislocations. To read more about
dislocations and TEM, I would suggest to take a look at Sir Peter Hirsch's
book . I think, it's called "Electron Microscopy of Thin Crystals".

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: mdhaque [mailto:mdhaque-at-students.uiuc.edu]
Sent: Friday, July 12, 2002 1:13 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

I have a question: With TEM how can I be sure that the feature I am looking
at
is/are dislocation(s)? Can you suggest any reading on dislocation
imaging..Thanks

Aman Haque

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Aman Haque
Dept of Mechanical & Industrial Engg
University of Illinois at Urbana Champaign

Tel: 217-244-2760, Fax: 217-244-6534



From daemon Fri Jul 12 18:52:47 2002



From: Ronald Vane :      RVane-at-Evactron.com
Date: Wednesday, July 10, 2002 10:21 PM
Subject: Re: Carbon Quantitation by EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, Steve, the much higher absorption coefficient for FE prevents the very
soft Boron X-rays from being emitted from FeB2 in sufficient quantity to be
detected.

Ron Vane
XEI Scientific

-----Original Message-----
} From: Steven Celotto {s.celotto-at-liverpool.ac.uk}
Cc: Microscopy Society of America {Microscopy-at-sparc5.microscopy.com}



From daemon Fri Jul 12 20:48:36 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 12 Jul 2002 21:24:47 -0400
Subject: TEM Dislocations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Another book that you might want to look at is Williams and Carter's book, Transmission Electron Microscopy. It is available from Plenum.

One slick method to determine the Burgers vector of a dislocation is to set up a multi-beam convergent beam diffraction pattern (e.g. three beams) with the beams at or very nearly at the exact Bragg condition and place the spot over the dislocation. You also have to have dynamical diffraction conditions so that you can see the HOLZ lines in the disks. If you don't see the HOLZ lines, then lower the voltage of the microscope. You will see that there are nodes in the HOLZ lines that appear to split the lines. If you know the g for a HOLZ line, the number of nodes in the line tells you the value of g dot b for that g (e.g. g * b=1,2,3,etc.). With three such disks, you have three equations and three unknowns and you can determine b from one pattern without the need to tilt the sample to other orientations to find g dot b = 0 conditions where the dislocation will disappear. See Spence and Zuo's book, Electron Microdiffraction, also available from Plenum.

If you use the tilting to find two-beam conditions where the dislocation disappears, two such g dot b =0 conditions, where the g's are not collinear, will give you b because it is mutually perpendicular to both g's.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Mike Bode [mailto:mb-at-Soft-Imaging.com]
Sent: Friday, July 12, 2002 7:18 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Dislocations have very specific visibility criteria in a TEM, which are
basically determined by their burgers vector. I am not close to my TEM books
right now, so I can't be more specific, but I am sure there are other people
here who are very familiar with dislocations. To read more about
dislocations and TEM, I would suggest to take a look at Sir Peter Hirsch's
book . I think, it's called "Electron Microscopy of Thin Crystals".

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: mdhaque [mailto:mdhaque-at-students.uiuc.edu]
Sent: Friday, July 12, 2002 1:13 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

I have a question: With TEM how can I be sure that the feature I am looking
at
is/are dislocation(s)? Can you suggest any reading on dislocation
imaging..Thanks

Aman Haque

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Aman Haque
Dept of Mechanical & Industrial Engg
University of Illinois at Urbana Champaign

Tel: 217-244-2760, Fax: 217-244-6534



From daemon Sat Jul 13 13:05:33 2002



From: zaluzec-at-microscopy.com
Date: Sat, 13 Jul 2002 12:16:19 -0500
Subject: M&M 2002 : Program Search Engine Now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues:

The Microscopy & Microanalysis 2002 Program Search Engine is now on-line at

http://www.msa.microscopy.com/

You may search the 2002 program by Author, Title, Symposium, Day of the Week.
to obtain a summary listing of presentations and posters.

The Complete Program listing is also available via a PDF File.

Feel free to forward this information along to anyone you think might
be interested.

Nestor
Your Friendly Neighborhood SysOp.


From daemon Sat Jul 13 13:51:58 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 14 Jul 2002 01:03:16 -0500
Subject: VEECO buys FEI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


.. And let's not forget Hull and Bacon, "Introduction to Dislocations" from
Pergamon Press. And I think, there's also information in L. Reimer,
"Transmission Electron Microscopy" from Springer.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Friday, July 12, 2002 7:25 PM
To: Microscopy (E-mail)
Cc: 'Mike Bode'


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

The following news item was in the July 13-14, 2002 International Herald
Tribune, sandwiched between articles about Enron and Worldcom:

SEMICONDUCTOR BUY:
Veeco Instruments, Inc. said it would purchase FEI Co., a maker of gear for
the semiconductor industry, for $1 billion in stock in a deal that would
make it the sixth-largest U. S. maker of chip-manufacturing equipment.

The acquisition is the latest in a string of acquisitions by Veeco, a maker
of precision instruments and electronic testing products, and signals
further consolidation in the semiconductor equipment market.

The purchase will give Veeco a larger presence in the business of
microscopic measurement systems used to make semiconductors, data storage
devices and other electronic products.

The Amsterdam-based Philips Electronics NV, the largest holder of FEI shares
with a 25% stake, will own about 15% of the combined company, a spokesman
said.

Reuters, Bloomberg


I thought at least some members of this listserver would find this
information quite interesting. We might also pause and ask whether this
kind of consolidation is positive, negative, or neutral for the future our
industry and market place.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================


From daemon Sun Jul 14 07:43:24 2002



From: Darryn Capes-Davis :      dcapes-davis-at-cmri.usyd.edu.au
Date: Sun, 14 Jul 2002 22:31:53 +1000
Subject: Test Post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry to post this but we have been having troubles posting and it may be
fixed now. So I am sending this as my staff have nothing actually to post at
the moment.

~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-
Darryn Capes-Davis BE
IT Manager
Children's Medical Research Institute
214 Hawkesbury Road
Westmead, NSW 2145
Australia
Tel.  +61 2 9687 2800
Fax  +61 2 9687 2120
Email  dcapes-davis-at-cmri.usyd.edu.au
~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-



From daemon Sun Jul 14 12:09:01 2002



From: zaluzec-at-microscopy.com
Date: Sun, 14 Jul 2002 11:56:02 -0500
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp


From daemon Sun Jul 14 12:09:18 2002



From: JHoffpa464-at-aol.com
Date: Sun, 14 Jul 2002 12:57:40 -0400
Subject: Re: Test Post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


test


From daemon Sun Jul 14 12:34:30 2002



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Sun, 14 Jul 2002 19:31:11 +0200
Subject: Re: TEM Dislocations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The exhaustive treatment of the "classical", two beam diffraction contrast
of dislocations in TEM is contained in the book of A.K.Head, P.Humble,
L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron
micrographs and defect identification". On the ground of the minimum
theoretical knowledge that any electron microscopist has to hold, it is
presented in all details, computer codes in Fortran included, the method
that can generate by computation the "classical" contrast of a rectilinear
dislocation and a complex of two dislocations and three stacking faults.
There are in use such computer programs, commercial and free ones, that
either are applying the codes given in that book or are providing more
advanced treatment dealing with the same problem.
It is surprising to see that the old "classical" knowledge of the
diffraction contrast interpretation has faded away during the HRTEM
offensive and that the necessity of looking back to the "simple" two beam
diffraction contrast is more and more a request.


Corneliu Sarbu, PhD
Department of Metallurgy and Applied Materials Science (MTM Dept.)
Catholic University of Leuven (KULeuven)
Kasteelpark ARENBERG nr. 44
B-3001 Heverlee-Leuven, Belgium
****************************************************************
Phone: +32-16-32.1241 - office
+32-16-32.1264 - secretary of department
Fax: +32-16-32.1992 or +32-16-32.1270
e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be
****************************************************************

At 14:13 12/07/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Jul 14 14:07:49 2002



From: Allen Sampson :      ars-at-sem.com
Date: Sun, 14 Jul 2002 13:56:28 -0700
Subject: RE: VEECO buys FEI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suspect history will repeat itself yet again. The two other EM
manufacturers that come to mind that sold because of their semiconductor
production products are ETEC and Amray.

ETEC's SEM business quietly folded twenty years ago, just a couple of years
after being sold to Perkin-Elmer primarily because of their electron beam
lithography products. ETEC was the original American SEM manufacturer, had
major market share here for years and put out a fine instrument. But, in
the end, sold out entirely to a manufacturer who wanted to augment their
optical stepper line of lithography equipment with the electron beam mask
makers from ETEC.

Amray's history in this regard is probably recent enough that most here
have at least heard some of it. While they have not pulled completely out
of the general EM business, what steps they have taken would seem to point
to their long term commitment to.

In both cases, customers were hurt. Not just by the loss of the
manufacturers, but also by the way their EM businesses were casually tossed
aside. Perhaps, this time, someone will at least make an effort to make it
painless for their current customers. I hesitate to think that anyone
would actually consider making an effort to maintain and improve their
general EM business, or spin it off as a separate division or company.

Given FEI's broader base of EM instruments, perhaps it will happen. I
wouldn't bet on it though.

There, a gauntlet thrown down to anyone from Veeco who may be reading this.
Please prove me wrong and keep this business going. You have a lot of
very loyal customers out there.

As far as whether such change is good, bad or indifferent, I think it's
just natural. Over the last few decades we've seen EM emerge from the
research labs to applications that intersect virtually every industry. The
growth of electron beam instruments has branched in many, often overlaying,
directions. This provides opportunities for some companies that can pio
neer new applications - applications that can often prove more profitable.

The good news is that EM has become such a pervasive and useful set of
technologies. Add to that the relatively high profit margin for
manufacturer's (particularly those just starting out), and you have a
formula for a constantly competitive environment. The more the industry
tries to consolidate, I believe, the more new manufacturer's we'll see.
The unfortunate thing is trying to keep up with all the name changes.

(A little disclaimer might be in order here. As many of you know, I am a
rather outspoken third-party service provider for EM and other instruments.
I never particularly liked the corporate environment of Philips in
general, but they have apparently done a very good job of keeping their
customers satisfied. In over twenty years of business, I have yet to work
on one of their instruments, and that's very rare. They've done a good
job, and I hate to see the good ones go.)


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Saturday, July 13, 2002 11:03 PM, Garber, Charles A.
[SMTP:cgarber-at-2spi.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} The following news item was in the July 13-14, 2002 International Herald
} Tribune, sandwiched between articles about Enron and Worldcom:
}
} SEMICONDUCTOR BUY:
} Veeco Instruments, Inc. said it would purchase FEI Co., a maker of gear
for
} the semiconductor industry, for $1 billion in stock in a deal that would
} make it the sixth-largest U. S. maker of chip-manufacturing equipment.
}
} The acquisition is the latest in a string of acquisitions by Veeco, a
maker
} of precision instruments and electronic testing products, and signals
} further consolidation in the semiconductor equipment market.
}
} The purchase will give Veeco a larger presence in the business of
} microscopic measurement systems used to make semiconductors, data storage
} devices and other electronic products.
}
} The Amsterdam-based Philips Electronics NV, the largest holder of FEI
shares
} with a 25% stake, will own about 15% of the combined company, a spokesman
} said.
}
} Reuters, Bloomberg
}
}
} I thought at least some members of this listserver would find this
} information quite interesting. We might also pause and ask whether this
} kind of consolidation is positive, negative, or neutral for the future
our
} industry and market place.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.2spi.com
} ########################
} ============================================
}
}
}
}



From daemon Sun Jul 14 17:09:23 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 14 Jul 2002 15:01:59 -0700
Subject: RE: VEECO buys FEI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may have an historical point here.

Amray is still alive and kicking. Even that they are
part of KLA-Tencor. The difference with them is that they
no longer support thermionic systems--just 305FE
Schottky gun systems. Since this is the same gun used
in the KLA CD-SEM systems, my hope is that SEM support
will continue for some time. I believe that KLA made
a major strategic blunder by exiting the general purpose SEM
market in favor of the high cost CD-SEMs. These giant
systems are all over the place at auctions and bone yards now
as the semi market has taken a nose dive. On the other hand
I don't see many used SEMs of any type come up for sale
at the rate of CD-SEMs. If KLA had kept the service going
for the non-FESEMs, they would have had a nice cash flow
instead of a loss.

They do not make lab SEMs any more. But the do service
the existing FESEMs. That is better than nothing. Mine is
very reliable and rarely needs service. Mostly, it is cleaning
and aperture replacement, and holder cleaning. I've heard
that all of the SEM technology is moving from MA to CA.
Perhaps parts supply will stay in MA. I'm not sure what the motivation
of this action is, but hopefully it will work out.

I'm working on a new XL-30 Sirion and am impressed by
how nicely it is built. But for me, the Amray is much easier to
use. Fast and efficient. Nice balance of computer control
and user interface. Probably just a personal preference.

Let's see what happens. You can bet that if the manufacturers
suffer economically, we users will too.

gary g.



At 01:56 PM 7/14/2002, you wrote:

} I suspect history will repeat itself yet again. The two other EM
} manufacturers that come to mind that sold because of their semiconductor
} production products are ETEC and Amray.
}
} ETEC's SEM business quietly folded twenty years ago, just a couple of years
} after being sold to Perkin-Elmer primarily because of their electron beam
} lithography products. ETEC was the original American SEM manufacturer, had
} major market share here for years and put out a fine instrument. But, in
} the end, sold out entirely to a manufacturer who wanted to augment their
} optical stepper line of lithography equipment with the electron beam mask
} makers from ETEC.
}
} Amray's history in this regard is probably recent enough that most here
} have at least heard some of it. While they have not pulled completely out
} of the general EM business, what steps they have taken would seem to point
} to their long term commitment to.
}
} In both cases, customers were hurt. Not just by the loss of the
} manufacturers, but also by the way their EM businesses were casually tossed
} aside. Perhaps, this time, someone will at least make an effort to make it
} painless for their current customers. I hesitate to think that anyone
} would actually consider making an effort to maintain and improve their
} general EM business, or spin it off as a separate division or company.
}
} Given FEI's broader base of EM instruments, perhaps it will happen. I
} wouldn't bet on it though.
}
} There, a gauntlet thrown down to anyone from Veeco who may be reading this.
} Please prove me wrong and keep this business going. You have a lot of
} very loyal customers out there.
}
} As far as whether such change is good, bad or indifferent, I think it's
} just natural. Over the last few decades we've seen EM emerge from the
} research labs to applications that intersect virtually every industry. The
} growth of electron beam instruments has branched in many, often overlaying,
} directions. This provides opportunities for some companies that can pio
} neer new applications - applications that can often prove more profitable.
}
} The good news is that EM has become such a pervasive and useful set of
} technologies. Add to that the relatively high profit margin for
} manufacturer's (particularly those just starting out), and you have a
} formula for a constantly competitive environment. The more the industry
} tries to consolidate, I believe, the more new manufacturer's we'll see.
} The unfortunate thing is trying to keep up with all the name changes.
}
} (A little disclaimer might be in order here. As many of you know, I am a
} rather outspoken third-party service provider for EM and other instruments.
} I never particularly liked the corporate environment of Philips in
} general, but they have apparently done a very good job of keeping their
} customers satisfied. In over twenty years of business, I have yet to work
} on one of their instruments, and that's very rare. They've done a good
} job, and I hate to see the good ones go.)
}
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} On Saturday, July 13, 2002 11:03 PM, Garber, Charles A.
} [SMTP:cgarber-at-2spi.com] wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} }
} } The following news item was in the July 13-14, 2002 International Herald
} } Tribune, sandwiched between articles about Enron and Worldcom:
} }
} } SEMICONDUCTOR BUY:
} } Veeco Instruments, Inc. said it would purchase FEI Co., a maker of gear
} for
} } the semiconductor industry, for $1 billion in stock in a deal that would
} } make it the sixth-largest U. S. maker of chip-manufacturing equipment.
} }
} } The acquisition is the latest in a string of acquisitions by Veeco, a
} maker
} } of precision instruments and electronic testing products, and signals
} } further consolidation in the semiconductor equipment market.
} }
} } The purchase will give Veeco a larger presence in the business of
} } microscopic measurement systems used to make semiconductors, data storage
} } devices and other electronic products.
} }
} } The Amsterdam-based Philips Electronics NV, the largest holder of FEI
} shares
} } with a 25% stake, will own about 15% of the combined company, a spokesman
} } said.
} }
} } Reuters, Bloomberg
} }
} }
} } I thought at least some members of this listserver would find this
} } information quite interesting. We might also pause and ask whether this
} } kind of consolidation is positive, negative, or neutral for the future
} our
} } industry and market place.
} }
} } Chuck
} }
} } ============================================
} }
} } Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} } President 1-800-2424-SPI
} } SPI SUPPLIES FAX: 1-610-436-5755
} } PO BOX 656 e-mail:cgarber-at-2spi.com
} } West Chester, PA 19381-0656 USA
} } Cust.Service: spi2spi-at-2spi.com
} }
} } Look for us!
} } ########################
} } WWW: http://www.2spi.com
} } ########################
} } ============================================
} }
} }
} }
} }



From daemon Sun Jul 14 17:43:55 2002



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Mon, 15 Jul 2002 08:34:17 +1000
Subject: Pacemakers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of our EM users is likely to need a heart pacemaker soon. Just to make
sure, we'd like to ask if there are any known problems associated with
pacemakers operating in the vicinity of TEMs and SEMs?

thanks
Sally



From daemon Sun Jul 14 23:51:13 2002



From: Majid Ghoddusi :      mghoddusi-at-cmri.usyd.edu.au
Date: Mon, 15 Jul 2002 14:40:53 +1000
Subject: test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



only a test, please ignore.





















.....................................................
Majid Ghoddusi, DVM PhD
Senior Microscopist
Children's Medical Research Institute
Locked Bag 23
Wentworthville NSW 2145
Tel: (02) 9687-2800
Fax:(02) 968702120
www.cmri.com.au
.....................................................





From daemon Mon Jul 15 01:14:44 2002



From: Majid Ghoddusi :      mghoddusi-at-cmri.usyd.edu.au
Date: Mon, 15 Jul 2002 16:06:05 +1000
Subject: test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



test only, please ignore.



















.....................................................
Majid Ghoddusi, DVM PhD
Senior Microscopist
Children's Medical Research Institute
Locked Bag 23
Wentworthville NSW 2145
Tel: (02) 9687-2800
Fax:(02) 968702120
www.cmri.com.au
.....................................................





From daemon Mon Jul 15 07:25:11 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 15 Jul 2002 08:20:10 -0400
Subject: Pacemakers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sally;

There are several variants in pacemakers. I worked on them back when in the
early models, fixed rate type [RCA/Cordis]. However, I would suggest to the
EM/TEM user that he consult the mfg. on the specific type he has and its'
precautions. If I'm not mistaken some have radio transmitters embedded for
remote monitoring purposes. EMP [Electromagnetic Pulse] is an issue but I
assume you aren't working on weapons systems or susceptibility of weapons
systems. From an operators perspective, just turning knobs and focusing, I
can't think of a reason why operating either instrument should be a problem.


Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Sally Stowe [mailto:stowe-at-rsbs.anu.edu.au]
Sent: Sunday, July 14, 2002 6:34 PM
To: microscopy-at-sparc5.microscopy.com


One of our EM users is likely to need a heart pacemaker soon. Just to make
sure, we'd like to ask if there are any known problems associated with
pacemakers operating in the vicinity of TEMs and SEMs?

thanks
Sally



From daemon Mon Jul 15 08:54:48 2002



From: zaluzec-at-microscopy.com
Date: Mon, 15 Jul 2002 08:42:58 -0500
Subject: Administrivia: Test & Out of the Office Messages.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues


Test Messages:
----------
Each test message that you post creates ~ 3000 email
messages which get sent world wide, and needlessly
fill up the Ether and the Email queues.

Please read the FAQ's and don't post any!!

I will work with you if you are having problems, but don't inflict
these problems on thousands of your colleagues. Most of the
time your posting problems can get sorted out
in a few days without hasseling the greater community.

99+% of the posting problems have to do with the Email
filters I have setup to minimize JUNK/SPAM mail.
These sometimes catch valid posting, with false positive
results of the filters subroutines. That is unavoidable.

You all have to put up with these filters, otherwise
this list will die from junk mail. Right now the
filter stops ~ 30+ junk postings/day. Unfortunately
I personally still see them all, in my feeble attempt to
keep this beast running ....

Remember: No attachments, No embedded HTML,Send
your message as pure & simple ASCII (plain) text in
the body of your message. And if your mail gets "rejected"
please READ the rejection message and follow the
instructions!!

Out of the Office Messages:
-------------------
In addition, you should unsubscribe if you leave the office for
a few days, instead of just turning on your "on vacation/out of
the office messages". These programs also generate needless Email
to the list, most of which gets captured, but some gets
through. This is also covered in the FAQ.



Nestor
Your Friendly Neighborhood SysOp


From daemon Mon Jul 15 09:50:16 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Mon, 15 Jul 2002 10:38:41 -0400
Subject: TMC ANTI-VIBRATION PLATFORM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a TMC MICRO-g Vibration Isolation System Model # 65-17171-01
that we wish to sell. The platform is 86" wide by 71" deep with a seating
cut out that is -at- 30" wide narrowing down to 12" and is about 38" deep.
System has 4 isolation feet. With the feet, it requires an area that is 97"
x 82". The weight of unit is between 2200 - 2500 lbs. Asking $4,000.00 or
best offer plus shipping and handling charges. Interested parties should
contact


Joe Goodhouse
Confocal / EM Core Laboratory Manager
Department of Molecular Biology
Princeton University
609-258-5432
jgoodhouse-at-molbio.princeton.edu

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Mon Jul 15 09:54:39 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Mon, 15 Jul 2002 09:47:34 -0500
Subject: RE: Carbon Quantitation by EDX

Contents Retrieved from Microscopy Listserver Archives
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Nevertheless, boron in borides (Fe or Cr) is easely
detectable with WDS. Have done it on SX50.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

}
}
} Yes, Steve, the much higher absorption coefficient for FE
} prevents the very
} soft Boron X-rays from being emitted from FeB2 in sufficient
} quantity to be
} detected.
}
} Ron Vane
} XEI Scientific
}
} -----Original Message-----
} } From: Steven Celotto {s.celotto-at-liverpool.ac.uk}
} Cc: Microscopy Society of America {Microscopy-at-sparc5.microscopy.com}
} Date: Wednesday, July 10, 2002 10:21 PM
} Subject: Re: Carbon Quantitation by EDX
}
}
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------
} ----------.
} }
} }
} } I am curious to hear the answer to this question from
} Jacques and I have
} } something to add.
} } Once I performed EDS analyses on a sample of BC particles on
} C film in
} order to
} } test a new EDS analysis system installed on a new FEG 200KeV
} TEM. After
} selecting
} } the correct time constant in the EDS software I could get nice
} distinguishable C
} } and B peaks. Later I tried doing a similar EDS analysis on large FeB2
} particles in
} }
} } a Fe matrix. I mention large here to stress that the
} particles went through
} the
} } specimen foil so most of the X-rays were being emitted
} directly from the
} particles
} }
} } without passing through the Fe matrix before reaching the
} detector. I never
} saw
} } even a hint of a B peak. These ppts should have contained 33at%B.
} } Was I doing something wrong or is that an indication of how
} easily boron
} X-rays
} } are absorbed within a sample containing large z atoms?
} }
} } Faerber Jacques wrote:
} }
} } }
} --------------------------------------------------------------
} ----------
} } } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} --------------------------------------------------------------
} ---------.
} } }
} } } Hi all
} } }
} } } In the same idee, what about mesurements on boron carbide
} ? Carbon AND
} } } boron concentrations ! I see nice spectras, without
} anything else ( a bit
} } } O, some times Al and N). And I have variations between
} samples in the B/C
} } } ratio. In such a case can I mesure only ratio variation, or is it
} possible
} } } to try some quantification (with standards). The sample is bulk B4C
} } } and laser ablation thick layers (with dropplets). Primary
} energy 3 to 5
} } } keV.
} } }
} } } I think it's a bit pretentious to quantify. What is
} other's opinion ?
} } }
} } } J. Faerber
} } } IPCMS-GSI
} } } (Institut de Physique et Chimie des Matériaux de Strasbourg
} } } Groupe Surface et Interfaces)
} } } 23, rue de Loess
} } } 67037 Strasbourg CEDEX
} } } France
} } }
} } } Tel 00 33(0)3 88 10 71 01
} } } Fax 00 33(0)0 88 10 72 48
} } } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
} } }
} } } On Tue, 9 Jul 2002, Ronald Vane wrote:
} } }
} } }
} }
} --------------------------------------------------------------
} ----------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
} --------------------------------------------------------------
} ---------.
} } } }
} } } }
} } } } Dear Chris and all:
} } } }
} } } } Quantitative analysis of Carbon by EDX is nearly
} impossible because of
} the
} } } } very shallow penetration depth of the Carbon X-rays. You
} really just
} measure
} } } } the surface. Surface analysis techniques such as XPS and
} Auger also
} show
} } } } that thin carbon films love to form on surfaces. If you
} have an XPS you
} use
} } } } your ion gun to sputter off the carbon surface scum to
} see the real
} surface.
} } } }
} } } } Dry ashing in a plasma cleaner can also remove the carbon surface
} layer.
} } } } Sputter etching can be used to remove gold and silver coatings.
} } } }
} } } } Ron Vane
} } } } XEI Scientific
} } } } 3124 Wessex Way, Redwood City, CA 94061
} } } } 650-369-0133
} } } } www.SEMCLEAN.com
} } } }
} } } } Note: XEI Scientific makes the EVACTRON plasma cleaning
} system for
} Electron
} } } } Microscope Chambers and FIBs, but does not make desk top
} plasma dry
} ashers
} } } } or sputter etchers.
} } } }
} } } } -----Original Message-----
} } } } } From: Chris Peppiatt {chris.peppiatt-at-nuigalway.ie}
} } } } To: Microscopy-at-sparc5.microscopy.com
} {Microscopy-at-sparc5.microscopy.com}
} } } } Date: Monday, July 08, 2002 3:22 PM
} } } } Subject: Carbon Quantitation by EDX
} } } }
} } } }
} } } }
} } -------------------------------------------------------------
} -----------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } -------------------------------------------------------------
} ----------.
} } } } }
} } } } }
} } } } } Dear All,
} } } } }
} } } } } Someone has asked me to do some work for them on
} mineral specimens.
} The
} } } } } samples will be mounted in resin and polished and we
} will then coat
} with
} } } } } carbon. Obviously we will set our software to
} deconvolute carbon from
} the
} } } } } analyses. We have the option to coat samples with either gold or
} silver and
} } } } } then look at the carbon content. I suppose the
} questions I would like
} to
} } } } } have answered are:
} } } } }
} } } } } (1) Heavy elements like gold or silver will absorb some
} of the light
} } } } } element (inc. carbon) X-rays when used as coatings. Is
} there any way
} of
} } } } } correcting for this to get an accurate quantitative
} analysis of carbon
} } } } content?
} } } } }
} } } } } (2) Is there any way of removing either gold/silver or
} carbon coatings
} from
} } } } } such samples except for the obvious method of
} regrinding/polishing the
} } } } } coating off?
} } } } }
} } } } } Thanks in advance.
} } } } }
} } } } } Regards,
} } } } }
} } } } } Chris Peppiatt
} } } } }
} } } } }
} } } } } ============================================
} } } } } Dr. Chris Peppiatt (Experimental Officer),
} } } } } The National Centre for Biomedical Engineering Science,
} } } } } Science & Engineering Technology Building,
} } } } } National University of Ireland Galway,
} } } } } Galway City, Co. Galway,
} } } } } Republic of Ireland.
} } } } } chris.peppiatt-at-nuigalway.ie
} } } } } Phone: +353 91 512157 Fax: +353 91 750596
} } } } } =============================================
} } } } }
} } } } }
} } } } }
} } } }
} } } }
} }
} } --
} } Dr. Steven Celotto
} } Department of Engineering
} } Materials Science & Engineering
} } University of Liverpool
} } Brownlow Hill
} } Liverpool L69 3BX
} } UNITED KINGDOM
} }
} } Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
} } Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
} } email: s.celotto-at-liv.ac.uk
} }
} }
} }
} }
}
}
}


From daemon Mon Jul 15 09:55:09 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 15 Jul 2002 10:32:57 -0400
Subject: Re: TEM Dislocations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Your posting reminded me of the software available at the MSA/MAS library. There is a public domain software program that is called "oneDis" (I think) that will simulate the image of a dislocation after providing the program with the imaging parameters. I would recommend that you donwload the software and "play" with it to learn what dislocations look like at different imaging conditions. It is quite useful and it will help with the original question that was posted.


You can get to the EMMPDL library from the MSA website. Here are instructions that I copied

EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National Laboratory. The following FTP Site give you access to these Software Libraries. Access is by anonymous Login:


Host : WWW.AMC.ANL.GOV or
the mirror site WWW.MSA.Microscopy.Com

Login:
Username = Anonymous
Password = Your Email Address







-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be]
Sent: Sunday, July 14, 2002 1:31 PM
To: Microscopy-at-sparc5.microscopy.com



The exhaustive treatment of the "classical", two beam diffraction contrast
of dislocations in TEM is contained in the book of A.K.Head, P.Humble,
L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron
micrographs and defect identification". On the ground of the minimum
theoretical knowledge that any electron microscopist has to hold, it is
presented in all details, computer codes in Fortran included, the method
that can generate by computation the "classical" contrast of a rectilinear
dislocation and a complex of two dislocations and three stacking faults.
There are in use such computer programs, commercial and free ones, that
either are applying the codes given in that book or are providing more
advanced treatment dealing with the same problem.
It is surprising to see that the old "classical" knowledge of the
diffraction contrast interpretation has faded away during the HRTEM
offensive and that the necessity of looking back to the "simple" two beam
diffraction contrast is more and more a request.


Corneliu Sarbu, PhD
Department of Metallurgy and Applied Materials Science (MTM Dept.)
Catholic University of Leuven (KULeuven)
Kasteelpark ARENBERG nr. 44
B-3001 Heverlee-Leuven, Belgium
****************************************************************
Phone: +32-16-32.1241 - office
+32-16-32.1264 - secretary of department
Fax: +32-16-32.1992 or +32-16-32.1270
e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be
****************************************************************

At 14:13 12/07/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jul 15 10:34:51 2002



From: hard-at-acsu.buffalo.edu
Date: Mon, 15 Jul 2002 11:27:03 -0500
Subject: Course Announcement (2nd Posting)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Course Announcement

Title: Optical Microscopy and Imaging in the Biomedical Sciences

When: October 9 - October 18, 2002

Where: Marine Biology Laboratory, Woods Hole, MA, USA

Tuition: $2200 (Includes room and board, text, handouts, supplies)

Application Deadline: July 25, 2002

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Internet: admissions-at-mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

Course Director: Colin S. Izzard, State University of New York -at- Albany
Phone: [518] 442 - 4367
EMail: csizzard-at-csc.albany.edu

Course Description:

For Whom:
Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and video-imaging will benefit greatly from this course as well.
There are no specific prerequisites, but an understanding of the basic
principles of optics is desirable. Limited to 24 students.

The eight day course consists of lectures, laboratory demonstrations,
exercises and discussions that will enable the participant to obtain and
interpret microscope images of high quality, to perform quantitative optical

measurements, and to produce photographic and video records for
documentation
and analysis.

Topics to be covered include:
principles of microscope design and image formation
bright field, dark field, phase contrast, polarized light,
differential
interference contrast, interference reflection, and fluorescence
microscopy
confocal scanning microscopy, multiphoton excitation fluorescence
microscopy and image deconvolution
digital image restoration and 3-D reconstruction
video imaging, recording, enhancement, and intensification
analog and digital image processing and analysis
fluorescent probes and ratiometric-imaging
laser tweezers and laser scissors

Applications to live cells will be emphasized; other specimens will be
covered as well.

Students will have direct hands-on experience with state-of-the-art
microscopes, video and digital cameras, recorders and image processing
equipment provided by major optical and electronics companies. Instruction
will be provided by experienced staff from universities and industry.

STUDENTS ARE ENCOURAGED TO BRING THEIR OWN BIOLOGICAL (PRIMARY
CULTURES, CELL LINES, PREPARED SLIDES, ETC.) AND MATERIAL SPECIMENS AND TO
USE THEM FOR COURSE EXERCISES, WHERE APPROPRIATE. Cell culture facilities
are available. Students are highly encouraged to discuss individual research
problems with the academic and commercial faculty.

For faculty list and additional information, see: http://www.mbl.edu




From daemon Mon Jul 15 10:41:40 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Mon, 15 Jul 2002 11:38:15 -0700
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hmm--- strictly, any
} processing *at all* corrupts the primary image data.

Looking at something corrupts it's nature and asking a question about it
eliminates other perpectives. Cats, electrons and trees falling in the
forest are all phenomena that illustrate the point that detection of
absolute truth is inherently flawed.


____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/



From daemon Mon Jul 15 11:19:15 2002



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Mon, 15 Jul 2002 16:12:03 -0400
Subject: Odd image effects

Contents Retrieved from Microscopy Listserver Archives
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Then by all means, let us stop looking and asking questions lest we
corrupt truth...

----- Original Message -----
} From: Michael Cammer {cammer-at-aecom.yu.edu}


Greetings all,

I routinely have to look at semiconductor devices from top down for
failure analysis. All has been fine with our images until we started
imaging with our new microscope.

To give some background, what we are looking at is aluminum metal lines with
tungsten interconnects standing proud of the aluminum. When we image what we
see is significant darkening of the image around groups of interconnects
and, in extreme cases, around individual interconnects.

We see this primarily when imaging in backscatter, but sometimes also using
In-lens and E-T secondary detectors. does anybody have a reasonable
explanation to what this phenomenon is?

Thanks for you time

Nick


From daemon Mon Jul 15 15:25:41 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Mon, 15 Jul 2002 16:16:55 -0400
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Unsubscribe jfmjfm-at-umich.edu
--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
USA
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"





From daemon Mon Jul 15 15:28:10 2002



From: Jamila Shawon :      Jamila_Shawon-at-student.uml.edu
Date: Mon, 15 Jul 2002 16:21:24 -0400
Subject: to purchase microtome machine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear all,

Currently we are looking for a microtome machine for our lab. Till now,
we use a microtome machine in another lab which is SORVAU & CAT 15350.
It has light source of 15400. We also like to have same type of
microtome with reasonable price.

So, any of you have any idea about the microtome, please let us know.
We will appreaciate that very much.

Thank you very much

Jamila Shawon



From daemon Mon Jul 15 16:42:56 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Mon, 15 Jul 2002 16:38:11 -0500
Subject: Texas Society for Microscopy Meeting Announcement and Call for Papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Texas Society for Microscopy
(http://www.texasmicroscopy.org/) will have its
Fall Meeting on Oct. 24-26, 2002 in Austin, TX at the
Embassy Suites Austin North Hotel .

The Thursday afternoon workshop will be on "TEM materials
preparation tools",
presented by Dr. Rocco Cerchiara, Applications Laboratory
Manager, E. A. Fischione.

The Friday afternoon workshop will be "Cryo-microtomy, with
emphasis on TEM
sample preparation", presented by Dr. Gregory Becker,
Product Manager,
RMC Products by Boeckeler.

Workshops sponsored by ASI, Inc. and TSM

There is also a call for papers for this meeting. All
technologies and disciplines involving
microscopy in any form are invited to contribute.

Please see our website, http://www.texasmicroscopy.org/ ,
for details, registration,
additional information, and author's instructions


--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-598-1291 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Mon Jul 15 17:19:16 2002



From: simkin-at-egr.msu.edu
Date: Mon, 15 Jul 2002 18:11:59 -0400 (EDT)
Subject: Re: Odd image effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nick,

It sounds as though you're getting a shadowing effect from the interconnects.
If a significant fraction of the backscattered electrons otherwise received by
your detector is blocked by the interconnects, then this will show up as
a darkening around these 'taller' features. This would be more pronounced for
W shadowing Al, as the BSEs coming off Al would tend to be comparatively low
energy, and thus more easily blocked by the W.
Assuming the geometry of the objects being imaged is the same as in your
previous 'scope, then it sounds as though either: 1) your collection
geometry for the new detector arrangement is larger (giving a larger solid angle
of collection in which some part of the signal is blocked), or 2) your new
detector has a different energy sensitivity as compared to the 'old' detector
(less sensitivity to lower energy electrons, OR a lower detection threshold
energy), or possibly even 3) the magnetic field strength in your chamber is
high enough to cause electrons emitted from your sample to spiral up about the
field lines to your detector, giving the same effect as if you had a larger
solid angle of collection. This last sounds like a possibility because of your
mention of an in-lens detector, which would tend to need a pretty strong
Z-axis magnetic field in order to operate.

Cheers,

Ben Simkin (simkin-at-egr.msu.edu)


} Greetings all,
}
} I routinely have to look at semiconductor devices from top down for
} failure analysis. All has been fine with our images until we started
} imaging with our new microscope.
}
} To give some background, what we are looking at is aluminum metal lines with
} tungsten interconnects standing proud of the aluminum. When we image what we
} see is significant darkening of the image around groups of interconnects
} and, in extreme cases, around individual interconnects.
}
} We see this primarily when imaging in backscatter, but sometimes also using
} In-lens and E-T secondary detectors. does anybody have a reasonable
} explanation to what this phenomenon is?
}
} Thanks for you time
}
} Nick


From daemon Mon Jul 15 17:47:55 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 15 Jul 2002 15:43:49 -0700
Subject: Re: Odd image effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hum...what is the new scope? What is the old scope?

What mag and KV are you using? Are the specimens
coated? With what and how much? What feature
sizes are you dealing with? Underneath the Al interconnects
should be TiW barrier metal if that process required it.
Z contrast will be high. If you are shooting with high KV,
large spot size and large aperture, I'd suspect charging.
Make sure that the specimen is grounded and is coated.
And use low KV (2-5) and a small probe diameter.

Posting a couple of sample images to your web site would
be helpful.

gary g.


At 01:12 PM 7/15/2002, you wrote:

} Greetings all,
}
} I routinely have to look at semiconductor devices from top down for
} failure analysis. All has been fine with our images until we started
} imaging with our new microscope.
}
} To give some background, what we are looking at is aluminum metal lines with
} tungsten interconnects standing proud of the aluminum. When we image what we
} see is significant darkening of the image around groups of interconnects
} and, in extreme cases, around individual interconnects.
}
} We see this primarily when imaging in backscatter, but sometimes also using
} In-lens and E-T secondary detectors. does anybody have a reasonable
} explanation to what this phenomenon is?
}
} Thanks for you time
}
} Nick



From daemon Mon Jul 15 19:45:36 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Sun, 14 Jul 2002 20:34:15 -0500
Subject: Looking for SDR unit for Philips 515 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I am looking for a Split Data Rotation unit (Part PW6762) for
a Philips 515 SEM. If you have such a device or know
where I can get one I would appreciate hearing from you.

Greg Barclay




From daemon Tue Jul 16 03:47:46 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 16 Jul 2002 08:40:27 -0400
Subject: Odd image effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

My lateral thoughts on the matter.

I don't think we corrupt the truth, it's more like we have a tendency to
produce corrupt representations of the truth.

We seek truth as best as we can, whilst recognising the limitations of

our methods (e.g. observation disturbs the object, sample preparation
induces artifacts, etc.)

and

ourselves (e.g. personal biases, the fact that we haven't considered all
possible explanations of the results etc.)

An honest and realistic scientist realises that he/she throws up as many
questions as he/she answers. Maybe in science, we just hope for better and
better approximations to the truth...

Of course, all this assumes that there is such an entity as "the truth",
independent of us, our thoughts, and our observations. Many philosophers
would deny that. I see no way that we can do science without this
assumption and we have to part company with some current philisophy as a
result. This assumption is of course deeply embedded in western culture and
very much part of the Judaeo-Christian and Muslim traditions.

Best wishes

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



----- Original Message -----
} From: {"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com}
To: "Michael Cammer" {cammer-at-aecom.yu.edu}
Cc: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, July 15, 2002 6:59 PM


Nicol;

What is the surface passivated with? If it is polyimide or
bisbenzocyclobutene you may get a little "burning" and definitely charging,
if uncoated. Also, hydrocarbons in your sample chamber will be, so to
speak, "painted" on the surface and look like the region you just rastered
on the SEM when you back out in mag.

A little more info. and someone may be able to help you with this.

Regards,

Peter Tomic
Anadigics, inc.

-----Original Message-----
} From: Nicol Aitken [mailto:nicol-at-semiconductor.com]
Sent: Monday, July 15, 2002 4:12 PM
To: microscopy-at-sparc5.microscopy.com


Greetings all,

I routinely have to look at semiconductor devices from top down for
failure analysis. All has been fine with our images until we started
imaging with our new microscope.

To give some background, what we are looking at is aluminum metal lines with
tungsten interconnects standing proud of the aluminum. When we image what we
see is significant darkening of the image around groups of interconnects
and, in extreme cases, around individual interconnects.

We see this primarily when imaging in backscatter, but sometimes also using
In-lens and E-T secondary detectors. does anybody have a reasonable
explanation to what this phenomenon is?

Thanks for you time

Nick


From daemon Tue Jul 16 10:10:59 2002



From: Kristian Ukkonen :      kristian.ukkonen-at-iki.fi
Date: Tue, 16 Jul 2002 19:47:32 +0300
Subject: Re: Fwd: legal requirements for microscope images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you Ian for a thoughtful and insightful contribution -- indeed,
one that allows us to continue to believe in the usefulness of our
endeavors to uncover various aspects of the truth that we hope is out
there.

Pragmatically, we can believe that this world is below the shadow of a
dream, and taught by time take it so -- excepting always steam.*

*Rudyard Kipling's "McAndrew's Hymn"


----- Original Message -----
} From: "Ian MacLaren" {maclaren-at-tu-darmstadt.de}



Michael Cammer wrote:
} } Hmm--- strictly, any
} } processing *at all* corrupts the primary image data.
}
} Looking at something corrupts it's nature and asking a question about it
} eliminates other perpectives. Cats, electrons and trees falling in the
} forest are all phenomena that illustrate the point that detection of
} absolute truth is inherently flawed.

http://angryflower.com/schrod.gif

Just the image for the lab fridge. :)

Kristian Ukkonen.



From daemon Tue Jul 16 14:54:38 2002



From: Yuquan Ding :      yding-at-uwaterloo.ca
Date: Tue, 16 Jul 2002 15:50:59 -0400
Subject: INCA Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

The last year we installed ENCA 200 system in our JSM 840 SEM. Last week
when we repaired our SEM, liquid nitrogen in dewar was emptied to remove
water. Then we re-filled new liquid nitrogen through "warm up" and "cool
down" procedures. After that, we tried to calibrate this ISIS system. When
making "discriminators only", a message showed "ISIS calibration unable to
measure strobe peak. Abandoning calibration". At somebody's suggestion,
several times of conditioner were conducted, but the situation is the same.
We couldn't do anything now. The system seems to be locked. Also when
running EDS, no any peaks could be found, showing very high deadtime (99%).
Even without beam, the deadtime is also very high. It was suspected that the
detector system was damaged. I suspect that the strobed zero peak is
generated in the XP2 pulse processor, not in the detector. Why
"discriminators only" didn't work?

If anybody of you has experience with this type of problems, I would like to
have your opinions and thoughts. Thanks!

Our system No. 05426-2390-414-51, Model 6506, att 1: 32804c802, att2:
0120024, window: ATW2 det. area 10 mmxmm.

Yuquan Ding
Materials Labs
Dept. of Mechanical Engineering
University of Waterloo
Waterloo, ON N2L 3G1
519-888-4567 x3766
Fax: 519-888-6197
Email: yding-at-uwaterloo.ca




From daemon Tue Jul 16 14:54:38 2002



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Tue, 16 Jul 2002 20:45:51 +0100
Subject: Re: TEM Dislocations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OneDis is the program developed by Head et al. It may be still in Fortran and it may still have its awkward input format (emulating the old punch card format). Prof. Veli-Tapani Kuokkala has repackaged the code into a user-friendly Windows format for PC. The program can still be download from his webpage.

http://www.tut.fi/units/ms/elm/enindex.htm

"Walck, Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Your posting reminded me of the software available at the MSA/MAS library. There is a public domain software program that is called "oneDis" (I think) that will simulate the image of a dislocation after providing the program with the imaging parameters. I would recommend that you donwload the software and "play" with it to learn what dislocations look like at different imaging conditions. It is quite useful and it will help with the original question that was posted.
}
} You can get to the EMMPDL library from the MSA website. Here are instructions that I copied
}
} EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National Laboratory. The following FTP Site give you access to these Software Libraries. Access is by anonymous Login:
}
} Host : WWW.AMC.ANL.GOV or
} the mirror site WWW.MSA.Microscopy.Com
}
} Login:
} Username = Anonymous
} Password = Your Email Address
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} P. O. Box 11472 (letters)
} Guys Run Rd. (packages)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8515 (fax)
}
} -----Original Message-----
} } From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be]
} Sent: Sunday, July 14, 2002 1:31 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: TEM Dislocations
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} The exhaustive treatment of the "classical", two beam diffraction contrast
} of dislocations in TEM is contained in the book of A.K.Head, P.Humble,
} L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron
} micrographs and defect identification". On the ground of the minimum
} theoretical knowledge that any electron microscopist has to hold, it is
} presented in all details, computer codes in Fortran included, the method
} that can generate by computation the "classical" contrast of a rectilinear
} dislocation and a complex of two dislocations and three stacking faults.
} There are in use such computer programs, commercial and free ones, that
} either are applying the codes given in that book or are providing more
} advanced treatment dealing with the same problem.
} It is surprising to see that the old "classical" knowledge of the
} diffraction contrast interpretation has faded away during the HRTEM
} offensive and that the necessity of looking back to the "simple" two beam
} diffraction contrast is more and more a request.
}
} Corneliu Sarbu, PhD
} Department of Metallurgy and Applied Materials Science (MTM Dept.)
} Catholic University of Leuven (KULeuven)
} Kasteelpark ARENBERG nr. 44
} B-3001 Heverlee-Leuven, Belgium
} ****************************************************************
} Phone: +32-16-32.1241 - office
} +32-16-32.1264 - secretary of department
} Fax: +32-16-32.1992 or +32-16-32.1270
} e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be
} ****************************************************************
}
} At 14:13 12/07/02 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi,
} }
} } I have a question: With TEM how can I be sure that the feature I am
} } looking at
} } is/are dislocation(s)? Can you suggest any reading on dislocation
} } imaging..Thanks
} }
} } Aman Haque
} }
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Aman Haque
} } Dept of Mechanical & Industrial Engg
} } University of Illinois at Urbana Champaign
} }
} } Tel: 217-244-2760, Fax: 217-244-6534

--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Tue Jul 16 15:49:41 2002



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Tue, 16 Jul 2002 16:37:37 -0400 (EDT)
Subject: TEM sperm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

One of our investigators wants to do TEM on a very minute quantity of
sperm, 40 of them to be exact. Does anyone have any suggestions for
preparing such a minute sample with the goal of actually being able to
find them upon ultrathin sectioning? So far we have tried suspending them
in a tiny drop of BSA, crosslinking the BSA with glutaraldehyde, and
treating it like a piece of tissue from that point on. Unfortunately,
looking for the sperm in the resulting tissue block is like trying to find
a pollywog in an ocean. Any helpful hints out there?

Thanks for your help.

Dotty


Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu
c



From daemon Tue Jul 16 16:27:05 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Tue, 16 Jul 2002 17:13:35 -0400
Subject: Biological Electron Microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Transmission Electron Microscopist

Princeton University's Department of Molecular Biology is seeking a
Transmission Electron Microscopist to run a new LEO912AB. Duties will
include: (1) teaching and training undergraduate and graduate students,
post-docs and faculty on instrument operation, (2) preparation and
sectioning of diverse biological specimens including yeast, virally infected
mammalian cells, brain slices and Drosophila embryos, using a variety of
methods including high-pressure freezing and freeze substitution techniques,
(3) daily microscope system checks and scheduling of users, (4) general lab
maintenance and supply. The candidate should have a BS/MS in a biological
field and several years of TEM experience. Knowledge of ultra-structural
and immuno-labeling protocols, the operation and care of microtomes and
other equipment needed for specimen preparation, a basic understanding of
digital imaging and the ability to manage and administer digital
workstations and image archives is essential. The candidate must be highly
interactive, willing to collaborate on diverse projects and able to identify
and research the best methods of specimen preparation and examination. Rank
and salary are dependent upon qualifications and experience. Please send
curriculum vitae, a list of references and representative samples of your
work to Professor Mark Rose, Chair, Search Committee, Department of
Molecular Biology, Princeton University, Princeton, NJ 08544-1014.
Princeton University is an Equal Opportunity/Affirmative Action Employer

Joe Goodhouse
Confocal / EM Core Lab Manager
Department of Molecular Biology
Princeton University

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Tue Jul 16 18:57:37 2002



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Tue, 16 Jul 2002 16:45:08 -0700
Subject: unsubscribe for a while

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe



From daemon Tue Jul 16 20:36:10 2002



From: zaluzec-at-microscopy.com
Date: Tue, 16 Jul 2002 20:24:03 -0500
Subject: Walter McCrone: 1916-2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

It is with sadness that I learned today of the death
on July 10th of Walter McCrone. Many of you will know of his
work in the area of applied light microscopy, particuliarly
in the area of materials analysis and problem solving as well
as the number of books/short course etc.. which bear
his name .

Additional details about his life and work at the following WWW Site

http://www.mccrone.org/WalterMcCrone.html


Walter McCrone is survived by his wife, Lucy, who is also an
accomplished microscopist.

Contributions can be made in his name to the Walter C. McCrone
Scholarship Fund for Advanced Microscopy Studies, c/o McCrone
Research Institute, 2820 S. Michigan Avenue, Chicago, IL 60616.


Regards...

Nestor


From daemon Tue Jul 16 22:29:03 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 16 Jul 2002 23:23:14 -0400
Subject: INCA Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mr. Ding;

Sounds like you either have a dead detector or the front end processing
electronics have failed. Dead time of 99% indicates something is radically
wrong. We had a similar situation recently with a EDX detector. The cause
was found to be a cracked detector window.

Peter

-----Original Message-----
} From: Yuquan Ding [mailto:yding-at-uwaterloo.ca]
Sent: Tuesday, July 16, 2002 3:51 PM
To: microscopy-at-sparc5.microscopy.com


Dear Listers:

The last year we installed ENCA 200 system in our JSM 840 SEM. Last week
when we repaired our SEM, liquid nitrogen in dewar was emptied to remove
water. Then we re-filled new liquid nitrogen through "warm up" and "cool
down" procedures. After that, we tried to calibrate this ISIS system. When
making "discriminators only", a message showed "ISIS calibration unable to
measure strobe peak. Abandoning calibration". At somebody's suggestion,
several times of conditioner were conducted, but the situation is the same.
We couldn't do anything now. The system seems to be locked. Also when
running EDS, no any peaks could be found, showing very high deadtime (99%).
Even without beam, the deadtime is also very high. It was suspected that the
detector system was damaged. I suspect that the strobed zero peak is
generated in the XP2 pulse processor, not in the detector. Why
"discriminators only" didn't work?

If anybody of you has experience with this type of problems, I would like to
have your opinions and thoughts. Thanks!

Our system No. 05426-2390-414-51, Model 6506, att 1: 32804c802, att2:
0120024, window: ATW2 det. area 10 mmxmm.

Yuquan Ding
Materials Labs
Dept. of Mechanical Engineering
University of Waterloo
Waterloo, ON N2L 3G1
519-888-4567 x3766
Fax: 519-888-6197
Email: yding-at-uwaterloo.ca




From daemon Wed Jul 17 02:30:35 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Wed, 17 Jul 2002 09:22:48 +0200
Subject: digest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


digest



From daemon Wed Jul 17 04:02:58 2002



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Wed, 17 Jul 2002 10:59:18 +0200
Subject: Re: TEM Dislocations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Just a short comment: the Fortran (i.e. the punch card) format is not
awkward at all. Besides, as long as the graphics is available in Fortran
too, I don't see why we should abandon the good old language which is still
very appropriate for scientific "FORmula TRANslation". There are a huge
amount of very good programs written in that language, still in use in the
scientific world. As an evidence I can mention a version of the codes given
in the book of Head et al., I have written myself, in Fortran, with screen
display of the graphica result included (written in Fortran too). Its
advantage is that it can be run even on old computers, under DOS. And they
are still a lot of "old" computers all over the world.

Corneliu Sarbu, PhD
Department of Metallurgy and Applied Materials Science (MTM Dept.)
Catholic University of Leuven (KULeuven)
Kasteelpark ARENBERG nr. 44
B-3001 Heverlee-Leuven, Belgium
****************************************************************
Phone: +32-16-32.1241 - office
+32-16-32.1264 - secretary of department
Fax: +32-16-32.1992 or +32-16-32.1270
e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be
****************************************************************


At 20:45 16/07/02 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jul 17 04:35:31 2002



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Wed, 17 Jul 2002 10:45:46 +0100
Subject: Re: TEM Dislocations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all
Did anyone ever rework these programs to handle dislocations in low symmetry
structures, e.g. monoclinic or triclinic?

Thanks

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/

----- Original Message -----
} From: "Steven Celotto" {s.celotto-at-liverpool.ac.uk}
Cc: "Microscopy (E-mail)" {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, July 16, 2002 9:45 PM


Perhaps I was not clear in saying what was awkward. It is their input format being
like that of a punch-card, not the Fortran language.
When I used their programs I had to make ascii/text files with the program
parameters (beam direction, line direction, operating, w, anno etc) in the 82 (?)
character line like when they had to input the data via punch cards. It was easy
for them because because they were 'brought up' with that format. I was amazed
that they could glance through the almost continuous strings of text and find
which parameter they wanted (and my mistakes). I had to count characters all the
time.

Corneliu Sarbu wrote:

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}
} Just a short comment: the Fortran (i.e. the punch card) format is not
} awkward at all. Besides, as long as the graphics is available in Fortran
} too, I don't see why we should abandon the good old language which is still
} very appropriate for scientific "FORmula TRANslation". There are a huge
} amount of very good programs written in that language, still in use in the
} scientific world. As an evidence I can mention a version of the codes given
} in the book of Head et al., I have written myself, in Fortran, with screen
} display of the graphica result included (written in Fortran too). Its
} advantage is that it can be run even on old computers, under DOS. And they
} are still a lot of "old" computers all over the world.
}
} Corneliu Sarbu, PhD
} Department of Metallurgy and Applied Materials Science (MTM Dept.)
} Catholic University of Leuven (KULeuven)
} Kasteelpark ARENBERG nr. 44
} B-3001 Heverlee-Leuven, Belgium
} ****************************************************************
} Phone: +32-16-32.1241 - office
} +32-16-32.1264 - secretary of department
} Fax: +32-16-32.1992 or +32-16-32.1270
} e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be
} ****************************************************************
}
} At 20:45 16/07/02 +0100, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } OneDis is the program developed by Head et al. It may be still in Fortran
} } and it may still have its awkward input format (emulating the old punch
} } card format). Prof. Veli-Tapani Kuokkala has repackaged the code into a
} } user-friendly Windows format for PC. The program can still be download
} } from his webpage.
} }
} } http://www.tut.fi/units/ms/elm/enindex.htm
} }
} } "Walck, Scott D." wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } Your posting reminded me of the software available at the MSA/MAS
} } library. There is a public domain software program that is called
} } "oneDis" (I think) that will simulate the image of a dislocation after
} } providing the program with the imaging parameters. I would recommend
} } that you donwload the software and "play" with it to learn what
} } dislocations look like at different imaging conditions. It is quite
} } useful and it will help with the original question that was posted.
} } }
} } } You can get to the EMMPDL library from the MSA website. Here are
} } instructions that I copied
} } }
} } } EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National
} } Laboratory. The following FTP Site give you access to these Software
} } Libraries. Access is by anonymous Login:
} } }
} } } Host : WWW.AMC.ANL.GOV or
} } } the mirror site WWW.MSA.Microscopy.Com
} } }
} } } Login:
} } } Username = Anonymous
} } } Password = Your Email Address
} } }
} } } -Scott
} } }
} } } Scott D. Walck, Ph.D.
} } } PPG Industries, Inc.
} } } Glass Technology Center
} } } P. O. Box 11472 (letters)
} } } Guys Run Rd. (packages)
} } } Pittsburgh, PA 15238-0472
} } }
} } } Walck-at-PPG.com
} } }
} } } (412) 820-8651 (office)
} } } (412) 820-8515 (fax)
} } }
} } } -----Original Message-----
} } } } From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be]
} } } Sent: Sunday, July 14, 2002 1:31 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: Re: TEM Dislocations
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } The exhaustive treatment of the "classical", two beam diffraction contrast
} } } of dislocations in TEM is contained in the book of A.K.Head, P.Humble,
} } } L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron
} } } micrographs and defect identification". On the ground of the minimum
} } } theoretical knowledge that any electron microscopist has to hold, it is
} } } presented in all details, computer codes in Fortran included, the method
} } } that can generate by computation the "classical" contrast of a rectilinear
} } } dislocation and a complex of two dislocations and three stacking faults.
} } } There are in use such computer programs, commercial and free ones, that
} } } either are applying the codes given in that book or are providing more
} } } advanced treatment dealing with the same problem.
} } } It is surprising to see that the old "classical" knowledge of the
} } } diffraction contrast interpretation has faded away during the HRTEM
} } } offensive and that the necessity of looking back to the "simple" two beam
} } } diffraction contrast is more and more a request.
} } }
} } } Corneliu Sarbu, PhD
} } } Department of Metallurgy and Applied Materials Science (MTM Dept.)
} } } Catholic University of Leuven (KULeuven)
} } } Kasteelpark ARENBERG nr. 44
} } } B-3001 Heverlee-Leuven, Belgium
} } } ****************************************************************
} } } Phone: +32-16-32.1241 - office
} } } +32-16-32.1264 - secretary of department
} } } Fax: +32-16-32.1992 or +32-16-32.1270
} } } e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be
} } } ****************************************************************
} } }
} } } At 14:13 12/07/02 -0500, you wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi,
} } } }
} } } } I have a question: With TEM how can I be sure that the feature I am
} } } } looking at
} } } } is/are dislocation(s)? Can you suggest any reading on dislocation
} } } } imaging..Thanks
} } } }
} } } } Aman Haque
} } } }
} } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } } } Aman Haque
} } } } Dept of Mechanical & Industrial Engg
} } } } University of Illinois at Urbana Champaign
} } } }
} } } } Tel: 217-244-2760, Fax: 217-244-6534
} }
} } --
} } Dr. Steven Celotto
} } Department of Engineering
} } Materials Science & Engineering
} } University of Liverpool
} } Brownlow Hill
} } Liverpool L69 3BX
} } UNITED KINGDOM
} }
} } Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
} } Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
} } email: s.celotto-at-liv.ac.uk

--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Wed Jul 17 05:34:07 2002



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Wed, 17 Jul 2002 11:25:44 +0100
Subject: Re: TEM Dislocations: low symmetry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No, as far as I know.
I have the Fortran/DOS versions for the cubic, tetragonal and hexagonal versions

in my draw. That is as low as they ever needed.
However, they show in their book how to modify the programs for tetragonal and
hexagonal crystals. In section 10.6 they explain how they designed the program
code so that modify it for other crystals can be done without massive amounts of

rewriting. And they explain how to do it giving the tetragonal and hexagonal
versions as examples. On the elastic constants in the ANCALC subroutine and the
'crystallography package' of the code needed to be modified.
If someone has the time and energy they could do it.



Ian MacLaren wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear all
} Did anyone ever rework these programs to handle dislocations in low symmetry
} structures, e.g. monoclinic or triclinic?
}
} Thanks
}
} Ian MacLaren
} N.B. New address
} TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
} Petersenstr. 23, 64287 Darmstadt, Germany
} Tel: +49 6151 162894
} ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/
}
} ----- Original Message -----
} } From: "Steven Celotto" {s.celotto-at-liverpool.ac.uk}
} Cc: "Microscopy (E-mail)" {microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, July 16, 2002 9:45 PM
} Subject: Re: TEM Dislocations
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } OneDis is the program developed by Head et al. It may be still in Fortran
} and it may still have its awkward input format (emulating the old punch card
} format). Prof. Veli-Tapani Kuokkala has repackaged the code into a
} user-friendly Windows format for PC. The program can still be download from
} his webpage.
} }
} } http://www.tut.fi/units/ms/elm/enindex.htm
} }
} } "Walck, Scott D." wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } Your posting reminded me of the software available at the MSA/MAS
} library. There is a public domain software program that is called "oneDis"
} (I think) that will simulate the image of a dislocation after providing the
} program with the imaging parameters. I would recommend that you donwload
} the software and "play" with it to learn what dislocations look like at
} different imaging conditions. It is quite useful and it will help with the
} original question that was posted.
} } }
} } } You can get to the EMMPDL library from the MSA website. Here are
} instructions that I copied
} } }
} } } EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National
} Laboratory. The following FTP Site give you access to these Software
} Libraries. Access is by anonymous Login:
} } }
} } } Host : WWW.AMC.ANL.GOV or
} } } the mirror site WWW.MSA.Microscopy.Com
} } }
} } } Login:
} } } Username = Anonymous
} } } Password = Your Email Address
} } }
} } } -Scott
} } }
} } } Scott D. Walck, Ph.D.
} } } PPG Industries, Inc.
} } } Glass Technology Center
} } } P. O. Box 11472 (letters)
} } } Guys Run Rd. (packages)
} } } Pittsburgh, PA 15238-0472
} } }
} } } Walck-at-PPG.com
} } }
} } } (412) 820-8651 (office)
} } } (412) 820-8515 (fax)
} } }
} } } -----Original Message-----
} } } } From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be]
} } } Sent: Sunday, July 14, 2002 1:31 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: Re: TEM Dislocations
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } The exhaustive treatment of the "classical", two beam diffraction
} contrast
} } } of dislocations in TEM is contained in the book of A.K.Head, P.Humble,
} } } L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron
} } } micrographs and defect identification". On the ground of the minimum
} } } theoretical knowledge that any electron microscopist has to hold, it is
} } } presented in all details, computer codes in Fortran included, the method
} } } that can generate by computation the "classical" contrast of a
} rectilinear
} } } dislocation and a complex of two dislocations and three stacking faults.
} } } There are in use such computer programs, commercial and free ones, that
} } } either are applying the codes given in that book or are providing more
} } } advanced treatment dealing with the same problem.
} } } It is surprising to see that the old "classical" knowledge of the
} } } diffraction contrast interpretation has faded away during the HRTEM
} } } offensive and that the necessity of looking back to the "simple" two
} beam
} } } diffraction contrast is more and more a request.
} } }
} } } Corneliu Sarbu, PhD
} } } Department of Metallurgy and Applied Materials Science (MTM Dept.)
} } } Catholic University of Leuven (KULeuven)
} } } Kasteelpark ARENBERG nr. 44
} } } B-3001 Heverlee-Leuven, Belgium
} } } ****************************************************************
} } } Phone: +32-16-32.1241 - office
} } } +32-16-32.1264 - secretary of department
} } } Fax: +32-16-32.1992 or +32-16-32.1270
} } } e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be
} } } ****************************************************************
} } }
} } } At 14:13 12/07/02 -0500, you wrote:
} } }
} } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi,
} } } }
} } } } I have a question: With TEM how can I be sure that the feature I am
} } } } looking at
} } } } is/are dislocation(s)? Can you suggest any reading on dislocation
} } } } imaging..Thanks
} } } }
} } } } Aman Haque
} } } }
} } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } } } Aman Haque
} } } } Dept of Mechanical & Industrial Engg
} } } } University of Illinois at Urbana Champaign
} } } }
} } } } Tel: 217-244-2760, Fax: 217-244-6534
} }
} } --
} } Dr. Steven Celotto
} } Department of Engineering
} } Materials Science & Engineering
} } University of Liverpool
} } Brownlow Hill
} } Liverpool L69 3BX
} } UNITED KINGDOM
} }
} } Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
} } Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
} } email: s.celotto-at-liv.ac.uk
} }
} }
} }

--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Wed Jul 17 06:13:29 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Wed, 17 Jul 2002 13:07:51 +0200
Subject: subscribe digest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subscribe digest



From daemon Wed Jul 17 06:59:00 2002



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Wed, 17 Jul 2002 08:51:58 -0300
Subject: Re: INCA problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hmm, must be a Canadian problem, because I have a somewhat similar situation.
We also have an Inca system with a SATW window. The JEOL serviceman
was just here for the routine on our 5600, and when he vented the column and
started taking it apart, we noticed a thin film of condensation on the outside
portion of the detector finger. The metal here is just cool to the touch - certainly
not cold enough to freeze. We didn't have any significant increase in boiling of the
LN2, and consumption is pretty much the rate it has always been. At first, I wasn't
too alarmed (humidity is nearly 100% in the Maritime summer), but then I got to
thinking (dangerous ground, I know) - why does this only occur when the scope is
vented? I've never noticed this condensation before, but normally the detector is
inserted fully into the column, and I don't see much of the finger on the outside of
the column in that instance.

I'm thinking that we have a pinhole leak or entirely broken window, and I hadn't
noticed it before because I normally do specimen exchanges very quickly, and use
the EDS relatively infrequently.

With the scope pumped back down, we got the same high deadtime, no peaks
in the spectra behavior described below, but this subsided after a few minutes. Low
energy peaks were somewhat depressed, but were restored after running the conditioning
routine. Strangely, I've had this high deadtime, no peak behavior before, even a month
or so after installation, usually shortly after specimen exchange. My questions to this oh so
experienced and wise group are:

1. Do I have a leak in the window? I probably know the answer to this one.

2. What are the long term effects on the detector of operating in this manner?
With normal use, I'm not so far appreciably affecting pumpdown time of the scope,
so I think with brief exchanges I'm not condensing that much inside the scope.

3. We use the EDS system fairly infrequently. Would it be better to warm the system
up and only cool it down when we need it? Or would it be better to keep
it cold and run the conditioning circuit before use?

Any help would be greatly appreciated. I'd like to dash right out and fix the thing - but
there is absolutely no money in the budget in the forseeable future for such a repair.
Any brave souls out there who have attempted repairing broken windows themselves?

Thanks in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America







From daemon Wed Jul 17 08:39:59 2002



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Wed, 17 Jul 2002 08:33:17 -0500
Subject: Re: INCA Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Yuquan

We have had similar problems with an 18month old Inca system this year. In
our case it was a cracked window and dead FET. The detector had to go back
to Oxford for repair.

Alan

At 03:50 PM 7/16/2002 -0400, Yuquan Ding wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Wed Jul 17 08:40:24 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 17 Jul 2002 09:28:16 -0400
Subject: Re: TEM sperm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} Hello,
}
} One of our investigators wants to do TEM on a very minute quantity of
} sperm, 40 of them to be exact. Does anyone have any suggestions for
} preparing such a minute sample with the goal of actually being able to
} find them upon ultrathin sectioning? So far we have tried suspending them
} in a tiny drop of BSA, crosslinking the BSA with glutaraldehyde, and
} treating it like a piece of tissue from that point on. Unfortunately,
} looking for the sperm in the resulting tissue block is like trying to find
} a pollywog in an ocean. Any helpful hints out there?
}
} Thanks for your help.
}
} Dotty
************************
Hi Dotty,
Your question is most timely from my perspective. I just ran up two
samples of sperm for a colleague. Initially, we tried agar, with the
same results you had with the BSA. My second, successful try
consisted simply of spinning the little guys down in an eppendorf
tube. Granted, I was using a 200 microliter suspension of sperm in
fix, and had many more than 40, but this worked well. I have a
small, table top centrifuge that I used at around 900g to bring them
to a pellet each time I changed solutions, and then an old Beckman
ultrafuge that holds the tubes horizontally to bring the pellet to
the very bottom when I put them into the resin. After osmium, I
could actually see the sample. With so few, you might want to pellet
them first, and then overlay them with a very small volume of the
BSA, just to hold them in place.

Good luck!
Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Jul 17 09:20:15 2002



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Wed, 17 Jul 2002 16:18:19 +0200
Subject: Re: TEM Dislocations: low symmetry

Contents Retrieved from Microscopy Listserver Archives
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I've never heard about an adaptation of this program for a lower symmetry than
the tetragonal one (which can be found in the original book by Head et al.).
As one who has implemented the Onedis code I have the feeling that it is not
very difficult to adapt it for such a lower symmetry. You have just to look
at the
subroutines that are involved in the calculation of the mechanical strain
field and do
the necessary modifications. The components of that strain field are much more
numerous as the symmetry gets lower, but it can be done. The code given in the
book of Head et al. is a good starting point, in as much as the basic
principles
of the calculations of each of the subroutines are thoroughly presented.

Corneliu Sarbu!

At 11:27 17/07/02 +0200, you wrote:
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From daemon Wed Jul 17 10:03:26 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 17 Jul 2002 09:55:36 -0500
Subject: Re: INCA problem

Contents Retrieved from Microscopy Listserver Archives
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James,
It sure sounds like you have a pinhole leak in your window. We developed
one in our Kevex Quantum detector on a JEOL 840A years ago. We only noticed
it when we had to vent the whole chamber for large samples. We normally
used the airlock and so the specimen chamber and detector were kept under
vacuum.

However, once we vented the column, we also vented the detector, at least
partially. That led to 99% deadtime until the air molecules could be pumped
back out the pinhole. Then things seemed to operate fine. I suppose a
person could keep on operating like that as routine, but contamination
might eventually build up on the crystal. We bit the bullet and shipped the
detector back for a window replacement that cost us somewhere between $2000
and 3000. It has been fine these many years since.

We normally see droplets of oil build up on our detector snout over time.
(That has been discussed here before.) After several months we take the
detector off the scope and clean it. We drizzle freon down the snout and
over the window. But you should check with your manufacturer to make sure
you don't do anything to damage your window (more) or dissolve the cement
holding the window in place.

I suppose water droplets could just as well build up. Iowa is humid, too,
but we vent with dry nitrogen and normally don't leave the chamber open all
that long, and the snout is not all that cool to the touch.

Warren

At 08:51 AM 7/17/02 -0300, you wrote:
} ------------------------------------------------------------------------
}
} Hmm, must be a Canadian problem, because I have a somewhat similar situation.
} We also have an Inca system with a SATW window. The JEOL serviceman
} was just here for the routine on our 5600, and when he vented the column and
} started taking it apart, we noticed a thin film of condensation on the outside
} portion of the detector finger. The metal here is just cool to the touch -
} certainly
} not cold enough to freeze. We didn't have any significant increase in
} boiling of the
} LN2, and consumption is pretty much the rate it has always been. At first,
} I wasn't
} too alarmed (humidity is nearly 100% in the Maritime summer), but then I
} got to
} thinking (dangerous ground, I know) - why does this only occur when the
} scope is
} vented? I've never noticed this condensation before, but normally the
} detector is
} inserted fully into the column, and I don't see much of the finger on the
} outside of
} the column in that instance.
}
} I'm thinking that we have a pinhole leak or entirely broken window, and I
} hadn't
} noticed it before because I normally do specimen exchanges very quickly,
} and use
} the EDS relatively infrequently.
}
} With the scope pumped back down, we got the same high deadtime, no peaks
} in the spectra behavior described below, but this subsided after a few
} minutes. Low
} energy peaks were somewhat depressed, but were restored after running the
} conditioning
} routine. Strangely, I've had this high deadtime, no peak behavior before,
} even a month
} or so after installation, usually shortly after specimen exchange. My
} questions to this oh so
} experienced and wise group are:
}
} 1. Do I have a leak in the window? I probably know the answer to this one.
}
} 2. What are the long term effects on the detector of operating in this manner?
} With normal use, I'm not so far appreciably affecting pumpdown time of the
} scope,
} so I think with brief exchanges I'm not condensing that much inside the scope.
}
} 3. We use the EDS system fairly infrequently. Would it be better to warm
} the system
} up and only cool it down when we need it? Or would it be better to keep
} it cold and run the conditioning circuit before use?
}
} Any help would be greatly appreciated. I'd like to dash right out and fix
} the thing - but
} there is absolutely no money in the budget in the forseeable future for
} such a repair.
} Any brave souls out there who have attempted repairing broken windows
} themselves?
}
} Thanks in advance,
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/~jehrman
}
}
}
} } ------------------------------------------------------------------------
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} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listers:
} }
} } The last year we installed ENCA 200 system in our JSM 840 SEM. Last week
} } when we repaired our SEM, liquid nitrogen in dewar was emptied to remove
} } water. Then we re-filled new liquid nitrogen through "warm up" and "cool
} } down" procedures. After that, we tried to calibrate this ISIS system. When
} } making "discriminators only", a message showed "ISIS calibration unable to
} } measure strobe peak. Abandoning calibration". At somebody's suggestion,
} } several times of conditioner were conducted, but the situation is the same.
} } We couldn't do anything now. The system seems to be locked. Also when
} } running EDS, no any peaks could be found, showing very high deadtime (99%).
} } Even without beam, the deadtime is also very high. It was suspected that the
} } detector system was damaged. I suspect that the strobed zero peak is
} } generated in the XP2 pulse processor, not in the detector. Why
} } "discriminators only" didn't work?
} }
} } If anybody of you has experience with this type of problems, I would like to
} } have your opinions and thoughts. Thanks!
} }
} } Our system No. 05426-2390-414-51, Model 6506, att 1: 32804c802, att2:
} } 0120024, window: ATW2 det. area 10 mmxmm.
} }
} } Yuquan Ding
} } Materials Labs
} } Dept. of Mechanical Engineering
} } University of Waterloo
} } Waterloo, ON N2L 3G1
} } 519-888-4567 x3766
} } Fax: 519-888-6197
} } Email: yding-at-uwaterloo.ca

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Wed Jul 17 10:11:34 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 17 Jul 2002 10:05:28 -0500
Subject: Re: subscribe digest

Contents Retrieved from Microscopy Listserver Archives
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If I may be so bold to stick my nose into what is normally Nestor's
business, I offer these sections taken from the list FAQ. The link to the
FAQ is given at the top of each posting.

Since Nestor's reminder to unsubscribe from the list rather than to simply
return "out of office" messages during absences, there have been a few
commands sent to the list to unsubscribe or to switch to digest mode. That
isn't the way to do it.

First, the list itself does not handle commands. Some implementations of
mailing lists do - many do not. This list simply is the latter form. See
note #2 below.

Second, there is no digest mode at this time. There may be later and I am
sure Nestor will tell us if digest mode becomes available.

Thanks, Nestor, for all your investment in this list. It is a wonderful aid.

Warren
------------------------------
How do I unsubscribe?

Send an Email Message to "Listserver-at-MSA.Microscopy.Com" in the body of
your message include the line
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Replace the "USERID-at-EMailAddress" with your real Email address.
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IMPORTANT NOTE#1. To unsubscribe you must supply the original address which
you used when you first submitted your subscription. Over the last few
years I have found that many users have their mail forwarded to new
addresses, or use an alias. When they then try to unsubscribe, they forget
where there mail is being sent to (or forwarded from). This causes me lots
of headaches. So just remember in order to unsubscribe you from the list,
the listserver must know the your original subscription address to remove it!
Please be considerate. I am not a mindreader, and don't really enjoy
playing detective over the Internet. I have too many other things to do.
IMPORTANT NOTE #2: Do not send subscribe/unsubscribe message to
"Microscopy-at-MSA.Microscopy.Com". When you do this ALL members of the
listserver receive your message. All administrative issues should be sent
to "Listserver-at-MSA.Microscopy.Com" and not to the general mailing list.


Is there a Digest Mode?

Digest Mode is not available on the present server. It is an option which
will be added in the future.
Digest Mode compresses all message from a single day into one long message
which is then sent to all subscribers. It is a nice option, and I intend to
get it running eventually.

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Wed Jul 17 10:40:19 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 17 Jul 2002 11:39:09 -0700
Subject: Re: TEM sperm

Contents Retrieved from Microscopy Listserver Archives
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Dorothy,
Some possibilities
a. fix and wick the cells into a cellulose fiber similar
to the one produced by Microdyn
(microfiltration cartridges)--will send website
directly
b. fix and drop cells onto a disk of nitrocellulose paper,
add second disk of np to sandwich cells
and process in wells of a staining dish
c. filter using polycarbonate filer, fix, process, flat
embedd or use a Chen mold

Rosemary
At 04:37 PM 7/16/02 -0400, Dorothy Roak Sorenson wrote:
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jul 17 11:18:56 2002



From: akc-at-umich.edu
Date: Wed, 17 Jul 2002 12:10:15 -0400
Subject: Re: TEM sperm

Contents Retrieved from Microscopy Listserver Archives
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Dotty,

I'm in a mountain cabin in Utah, looking at my e-mail on a laptop.

That's a tough problem. I could imagine something like the following:
Make up plastic embedding monomer and add a little dye to it. Polymerize a
block of it and then make tiny shavings or particles of it. Put some
sticky substance (polylysine, etc.?) on the surface of the shavings. Put
one or more shaving in with the sperm in a way that would cause the sperm
to attach to the shaving(s). You would have to decide whether the sperm
should be fixed before or after they attached to the shaving. When fixed
sperm are on the shaving(s), dehydrate them, and then put a shaving or
tight cluster of shavings into embedding medium at one end of a flat
embedding block. Polymerize the block. You should be able to see the
shaving(s) because of the dye, and can section them and see the sperm that
are on theeir surface.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Tuesday, July 16, 2002 4:37 PM -0400 Dorothy Roak Sorenson
{dsoren-at-umich.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} One of our investigators wants to do TEM on a very minute quantity of
} sperm, 40 of them to be exact. Does anyone have any suggestions for
} preparing such a minute sample with the goal of actually being able to
} find them upon ultrathin sectioning? So far we have tried suspending them
} in a tiny drop of BSA, crosslinking the BSA with glutaraldehyde, and
} treating it like a piece of tissue from that point on. Unfortunately,
} looking for the sperm in the resulting tissue block is like trying to find
} a pollywog in an ocean. Any helpful hints out there?
}
} Thanks for your help.
}
} Dotty
}
}
} Dotty Sorenson
} Microscopy and Image Analysis Laboratory
} Department of Cell and Developmental Biology
} University of Michigan Medical School
} Ann Arbor, Michigan
} (734)763-1170
} FAX (734)763-1166
} dsoren-at-umich.edu
} c
}







From daemon Wed Jul 17 13:16:44 2002



From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Wed, 17 Jul 2002 11:13:20 -0700
Subject: Re: TEM sperm

Contents Retrieved from Microscopy Listserver Archives
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You might try mixing a small drop of stain such as Toluidine Blue in the
agar for easier visualization of your sample.

At 04:37 PM 7/16/02 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Larry Ackerman
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Avenue
San Francisco, CA 94143 USA

415-476-8751 FAX 415-476-5774

http://www.ucsf.edu/jan



From daemon Wed Jul 17 16:51:06 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 17 Jul 2002 14:38:16 -0700
Subject: TEM sperm

Contents Retrieved from Microscopy Listserver Archives
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Have you thought about using a FIB?

John Mardinly
Intel



-----Original Message-----
} From: Dorothy Roak Sorenson [mailto:dsoren-at-umich.edu]
Sent: Tuesday, July 16, 2002 1:38 PM
To: microscopy-at-sparc5.microscopy.com


Hello,

One of our investigators wants to do TEM on a very minute quantity of
sperm, 40 of them to be exact. Does anyone have any suggestions for
preparing such a minute sample with the goal of actually being able to
find them upon ultrathin sectioning? So far we have tried suspending them
in a tiny drop of BSA, crosslinking the BSA with glutaraldehyde, and
treating it like a piece of tissue from that point on. Unfortunately,
looking for the sperm in the resulting tissue block is like trying to find
a pollywog in an ocean. Any helpful hints out there?

Thanks for your help.

Dotty


Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu
c



From daemon Wed Jul 17 16:57:54 2002



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Wed, 17 Jul 2002 15:54:56 -0300 (ADT)
Subject: TEM-hepes buffer

Contents Retrieved from Microscopy Listserver Archives
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Hi Everybody!
I was wondering if any of you have used Hepes buffer for electron
microscopy fixation. We used 2% glut in Hepes and we found
fixation unsatisfactory. Did any of you experienced any problems
using this system?
Thanks
Dorota


From daemon Wed Jul 17 19:07:05 2002



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Thu, 18 Jul 2002 11:59:24 +1200
Subject: Flat embedding using Melinex problem

Contents Retrieved from Microscopy Listserver Archives
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Kia Ora

A flat embedding problem. We have cryofixed and cryosubstituted some
Pisolithus tinctorius (a fungi) for one of our users and have run
into a a bit of a problem.

We cryosubsituted in 2% Osmium Tetroxide in Acetone for 72 hours in a
Leica AFS. After gradually bringing the samples to room temperature
and rinsing in absolute acetone we very slowly infiltrated into
Quetol 651 resin (formulation used; Quetol 651 10 g, NSA 10g, MNA 10g
DMP-30 0.45g). We then flat embedded the fungi between two sheets of
Melinex film (Agar Scientific catalogue number L4103) and polymerised
overnight at 60oC.

We have used Melinex film on numerous occasions in the past to flat
embed vibratomed sectioned brain slices, monolayers of culture cells
etc. We have used Melinex film with 'conventional' epoxy resins (ie
TAAB TK3, Agar 100 resin) and with LR White. We have not in the past
used Melinex with Quetol 651.

When we came to seperate the Melinex to remove the flat embedded
fungi the Melinex crazed and fractured , it wouldn't peel apart.

We ran a trial with old stock Melinex and some fairly new stuff. Not
wanting to get caught again we also trialed with some Alcar, Thermonx
and Mylar film. We tried the Quetol 651 again and some Agar 100.

Interestingly the Meinex (both the old stock and new stuff) reacted
in the same way both with the Quetol and the Agar 100 (which has
nevcer happened to us before). The Alcar, Thermonx and Mylar film
were all fine with the two resins.

Is, or has, anyone else out experienced the same problem and come up
with an answer ?

While on the subject, does anybody know at what temperature the
osmium in the acetone based cryosubstitution medium actual fixes at ?

It is my understanding that the idea is that the acetone/ osmium
fully infiltrates the tissue at the lower temperature, replacing the
cell water, but the osmium doesn't actually fix until the sample is
warmed up to a particular temperature (warmed up still being somewhat
lower than zero degress C).

Thanks in advance

Allan




--
-------------------------------------------------
Allan Mitchell
Technical Manager
Otago Centre for Electron Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Thu Jul 18 01:11:00 2002



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Thu, 18 Jul 2002 16:00:17 +1000
Subject: Ultrastructure of boab nuts?

Contents Retrieved from Microscopy Listserver Archives
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We have a student interested in the ultrastructure of boab nuts....can
anyone help with the interpretation of micrographs?

cheers
Sally


Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU




From daemon Thu Jul 18 07:48:48 2002



From: Katherine.S.Connolly-at-dartmouth.edu (Katherine S. Connolly)
Date: 18 Jul 2002 08:38:21 EDT
Subject: TEM sperm

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I would do all steps in a centrufuge.
Kate Connolly


From daemon Thu Jul 18 08:53:17 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 18 Jul 2002 09:44:07 -0400
Subject: RE: TEM-HEPES buffer

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Morning,

When you changed your buffer from the old(?) to HEPES, what change,
if any, did you record in its osmolarity? Also, there ARE non-trivial
effects recorded for different buffering systems on various tissues. Every
change should be followed by an experiment to define new optima for the
tissues under consideration, and the best way to determine new optima is to
start with semi-thin section comparisons followed by ultrastructural
analysis.

If it was easy, we'd all be paid much more for our work.

Regards,


Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Dorota Wadowska
} Sent: Wednesday, July 17, 2002 2:54 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM-hepes buffer
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Everybody!
} I was wondering if any of you have used Hepes buffer for electron
} microscopy fixation. We used 2% glut in Hepes and we found
} fixation unsatisfactory. Did any of you experienced any problems
} using this system?
} Thanks
} Dorota
}
}


From daemon Thu Jul 18 10:22:35 2002



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Thu, 18 Jul 2002 10:14:01 -0500 (CDT)
Subject: Re: TEM-hepes buffer

Contents Retrieved from Microscopy Listserver Archives
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Hi Dorrota,

I've used HEPES buffer with eubacteria, cyanobacteria, several algae
(green and golden brown) and land plants with good success. I did a
side-by-side comparison of cacodylate, phosphate and PIPES (a different
organic buffer) early in my career - an increasingly more scary number of
years ago - and PIPES was by far the best. I tried HEPES on the advice of
a collegue that worked on dinoflagellates, and have been using it ever
since. I have not had much experience with animal tissue, with the
exception of some tissue cultured cells. We have used HEPES in these
situations when it was the buffer system the cells were grown in, and have
had no trouble with it.

Heather Owen


Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall, P.O. Box 413
Milwaukee, WI 53210
USA

Phone: (414)229-6816



From daemon Thu Jul 18 10:55:30 2002



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 18 Jul 2002 11:49:34 -0600
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
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ELECTRON MICROSCOPIST/ RESEARCH ASSOCIATE

YALE UNIVERSITY SCHOOL OF MEDICINE


Electron Microscopy Core Facility at the Yale University School of
Medicine Seeks experienced electron microscopist to assist Principal
Investigator and other research scientists as consultant for independent
experiments; overseeing the operation of the facility, preparation of
specimens, conducting immunocytochemical labeling, sample analysis and
statistical analysis. Candidate will be expected to conduct independent
research projects in consultation of PI; design experiments, interpret
results and present proposals for future experiments. Will also
supervise lab personnel and other users of the facility as well as teach
EM techniques to users on a 1:1 basis or formal courses organized
biannually.

Position requires a Master’s degree in the biological sciences and one
year prior experience in related position; or the equivalent combination
of education and experience. Previous experience in microscopy and in
the conduct of independent research is required; also strong computer
literacy is required.

Qualified candidates send resume and cover letter referencing source
code EASEM10769 to Ms. S. Greer at jobs-at-yale.edu. Fax: 203-432-9817.
Mail: PO Box 208344, New Haven, CT 06520-8344. For more information
about this position and Yale’s outstanding benefits package visit
www.yale.edu.

Yale University is an Affirmative Action, Equal Opportunity Employer.

--
Marc Pypaert
Department of Cell Biology,
Center for Cell and Molecular Imaging and
Ludwig Institute for Cancer Research,
Yale University School of Medicine
333 Cedar Street
New Haven CT 06520
Tel : (203) 785 3681
Fax : (203) 785 7446




From daemon Thu Jul 18 11:43:17 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 18 Jul 2002 12:40:28 -0700
Subject: Re: TEM-hepes buffer

Contents Retrieved from Microscopy Listserver Archives
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Dorota,
We obtained good fixation of Arabidopsis thaliana buds using the
protocol from the following publication:
Owen HA and Makaroff CA. Ultrastructure of microsporogenesis and
microgametogenesis in Arabidopsis thaliana (L.) Heynh. ecotype
Wassilewskija (Brassicaceae). Protoplasma 185:7-21, 1995.
I don't have concentrations available to me at the moment but the
fixatives are fully described in this article. It was a great find for us
and we have used it on other plants as well.
Rosemary




From daemon Thu Jul 18 12:46:03 2002



From: JHoffpa464-at-aol.com
Date: Thu, 18 Jul 2002 13:37:15 -0400
Subject: Re: TEM-HEPES buffer

Contents Retrieved from Microscopy Listserver Archives
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if it was easy any HS student could do it and we would all be making min wage.
john


From daemon Thu Jul 18 12:56:07 2002



From: Lu-Chang Qin :      lcqin-at-physics.unc.edu
Date: Thu, 18 Jul 2002 13:48:59 -0400
Subject: broken spare TEM sample holder

Contents Retrieved from Microscopy Listserver Archives
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I am looking for a broken TEM sample holder that you may no longer need.
Please contact me if you have one - thanks in advance.

With best regards.
-----------------------------------------------------------------
Lu-Chang Qin, ScD
Department of Physics and Astronomy &
Curriculum in Applied and Materials Sciences
University of North Carolina at Chapel Hill
Room 178 Phillips Hall, CB#3255
Chapel Hill, NC 27599-3255
Phone: (919) 843-3575



From daemon Thu Jul 18 13:50:25 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 18 Jul 2002 12:02:11 -0700
Subject: RE: TEM-HEPES buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was surprised to find that some people in EM Labs used 0.1 M phosphate
buffer as a "PBS". From my course of biochemistry/cell biology PBS stands
for Phosphate Buffered Saline and is 20 mM phosphate buffer, pH 7.4 and 150
mM NaCl. If you replace 20 mM phosphate on 20 mM of any other buffer,
HEPES for instance, it's very unlikely the total osmomolarity will
changed... BUT, if you are using 0.1 (?) M HEPES it may be a HUGE
DIFFERENCE... You have to understand clearly, which buffer system you are
using. In biochemistry, we never ever use 100 mM range buffers itself (like
0.1 M phosphate/HEPES/MOPS etc). The purpose of the 'buffer' is to provide
necessary pH and sodium or potassium salts were used to provide necessary
osmosity... GA itself affect osmosity seriously and formally speaking,
osmosity should be corrected. From the 'chemical' point of view, HEPES
should not affect GA efficiency. Moreover, HEPES is the best buffer for GA
crosslinking because of it's huge buffer capacity in the neutral pH range.
As I understand, some 'buffer systems' like cacodylate helps to wash out
some content of the cell matrix and therefore makes some structural details
more pronounced. Mistakenly, some people called it 'good fixation'. If you
are using 100 mM range buffers, such 'washing effect' supposed to be very
pronounced. HEPES is good to preserve the matrix integrity, not for
washing out it's components. I am sorry for such obvious comment. Best
wishes, Sergey.

At 09:44 AM 7/18/02 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jul 18 14:05:10 2002



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Thu, 18 Jul 2002 14:58:39 -0400
Subject: Hand-held GFP detection devices???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone,

Rather than putting animals (such as mouse pups) under a
stereoscope that can detect GFP, has anyone ever heard of a hand-held
device that can be used to see if the pups are green?

Thank you in adance...

Lesley



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Thu Jul 18 15:55:25 2002



From: kwalters :      kwalters-at-blue.weeg.uiowa.edu
Date: Thu, 18 Jul 2002 15:47:17 -0500
Subject: antibodies for porcine sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I have a project requiring that I identify cells grown in culture that were
originally taken from porcine tissue. The cells are suspected to be either
smooth muscle cells, endothelium, or fibroblasts. I am unfamiliar with which
antibodies would work for these cells. There are many antibodies for these
types of cells, but will they work in porcine tissue? Help.....


Thanks,
Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------



From daemon Thu Jul 18 18:13:30 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 18 Jul 2002 16:01:27 -0700
Subject: RE: TEM-HEPES buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Not at all Fred. I was commenting the original message, not yours. No
worry. I think, we both agree that in order to get good answer, we have to
ask good questions. For instance the definition of the 'HEPES buffer' to
me mean that people talking about 10-20 mM concentration of HEPES. 0.1 M
HEPES is a stock solution to me. Others may think differently. As a result
there is misunderstanding may occur (exactly what you mean). Sergey


At 01:29 PM 7/18/02, you wrote:
} Did I say something wrong? I tried not to. But I can tell from what you
} wrote that we agree. My response was my nasty way to say that there was
} insufficient information to draw any conclusion and frame any answer.
}
} Hope you agree.
}
} Fred
}
}
} } ----------
} } From: Sergey Ryazantsev
} } Sent: Thursday, July 18, 2002 3:02 PM
} } To: Monson, Frederick C.; microscopy-at-sparc5.microscopy.com
} } Subject: RE: TEM-HEPES buffer
} }
} } I was surprised to find that some people in EM Labs used 0.1 M phosphate
} } buffer as a "PBS". From my course of biochemistry/cell biology PBS
} } stands
} } for Phosphate Buffered Saline and is 20 mM phosphate buffer, pH 7.4 and
} } 150
} } mM NaCl. If you replace 20 mM phosphate on 20 mM of any other buffer,
} } HEPES for instance, it's very unlikely the total osmomolarity will
} } changed... BUT, if you are using 0.1 (?) M HEPES it may be a HUGE
} } DIFFERENCE... You have to understand clearly, which buffer system you are
} }
} } using. In biochemistry, we never ever use 100 mM range buffers itself
} } (like
} } 0.1 M phosphate/HEPES/MOPS etc). The purpose of the 'buffer' is to
} } provide
} } necessary pH and sodium or potassium salts were used to provide necessary
} } osmosity... GA itself affect osmosity seriously and formally speaking,
} } osmosity should be corrected. From the 'chemical' point of view, HEPES
} } should not affect GA efficiency. Moreover, HEPES is the best buffer for
} } GA
} } crosslinking because of it's huge buffer capacity in the neutral pH range.
} }
} } As I understand, some 'buffer systems' like cacodylate helps to wash out
} } some content of the cell matrix and therefore makes some structural
} } details
} } more pronounced. Mistakenly, some people called it 'good fixation'. If
} } you
} } are using 100 mM range buffers, such 'washing effect' supposed to be very
} } pronounced. HEPES is good to preserve the matrix integrity, not for
} } washing out it's components. I am sorry for such obvious comment. Best
} } wishes, Sergey.
} }
} } At 09:44 AM 7/18/02 -0400, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Morning,
} } }
} } } When you changed your buffer from the old(?) to HEPES, what
} } change,
} } } if any, did you record in its osmolarity? Also, there ARE non-trivial
} } } effects recorded for different buffering systems on various tissues.
} } Every
} } } change should be followed by an experiment to define new optima for the
} } } tissues under consideration, and the best way to determine new optima is
} } to
} } } start with semi-thin section comparisons followed by ultrastructural
} } } analysis.
} } }
} } } If it was easy, we'd all be paid much more for our work.
} } }
} } } Regards,
} } }
} } }
} } } Fred Monson
} } }
} } } Frederick C. Monson, PhD
} } } Center for Advanced Scientific Imaging
} } } Schmucker II Science Center
} } } West Chester University
} } } South Church Street and Rosedale
} } } West Chester, Pennsylvania, USA, 19383
} } } Phone: 610-738-0437
} } } FAX: 610-738-0437
} } } fmonson-at-wcupa.edu
} } } CASI URL: http://darwin.wcupa.edu/casi/
} } } WCUPA URL: http://www.wcupa.edu/
} } } Visitors URL: http://www.wcupa.edu/_visitors/
} } }
} } }
} } } } ----------
} } } } From: Dorota Wadowska
} } } } Sent: Wednesday, July 17, 2002 2:54 PM
} } } } To: microscopy-at-sparc5.microscopy.com
} } } } Subject: TEM-hepes buffer
} } } }
} } } }
} } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi Everybody!
} } } } I was wondering if any of you have used Hepes buffer for electron
} } } } microscopy fixation. We used 2% glut in Hepes and we found
} } } } fixation unsatisfactory. Did any of you experienced any problems
} } } } using this system?
} } } } Thanks
} } } } Dorota
} } } }
} } } }
} }
} }

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jul 18 21:28:04 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 18 Jul 2002 19:23:35 -0700
Subject: XL-30 Sirion x-ray & diffusion pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone out there have any experience to share
about the Philips XL-30 (Sirion or other) with EDAX relative
to potential consequences of a diffusion pump versus
a turbo? It seems to me that a turbo pump would
go a long way to prevent contamination of the
detector window. I am not sure about this. The
Sirion isolates the chamber from the diffusion pump
during specimen exchange. Plus, it has a chilled
water trap. So this may not be a problem.

gg



From daemon Thu Jul 18 23:52:43 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Fri, 19 Jul 2002 14:47:35 +1000
Subject: Re: Hand-held GFP detection devices???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Haven't tried this yet, but a blue fluorescent lamp, maximum emission
around 480 or so nm, may excite GFP enough to be visible in a dark room.
We're planning to test this to see if we can screen thousands of
GFP-expressing seeds. Could observe the fluorescence through a long-pass
theatre filter of some kind, like one of the Lee green or yellow filters,
which have quite sharp wavelength cutoffs (have used various Lee filters to
get different wavelength illumination for prac classes looking at
photomorphogenesis in plants).
good luck,
Rosemary
}
}
} Hi Everyone,
}
} Rather than putting animals (such as mouse pups) under a
} stereoscope that can detect GFP, has anyone ever heard of a hand-held
} device that can be used to see if the pups are green?
}
} Thank you in adance...
}
} Lesley
}
}
}
} Lesley S. Bechtold
} Supervisor, Biological Imaging
} The Jackson Laboratory
} 600 Main St.
} Bar Harbor, ME 04609
} 207-288-6191


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia
rosemary.white-at-csiro.au
fax 61- 2 6246 5000
ph. 61- 2 6246 5475 or
mob. 61- 0402 835 973




From daemon Fri Jul 19 05:38:26 2002



From: Renaat Dasseville :      renaat.dasseville-at-rug.ac.be
Date: Fri, 19 Jul 2002 13:36:07 +0200
Subject: SEM Peltier cold stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kristian
Thanks for that brief frisson of levity.
I failed to observe the promised cartoon on
the double slit experiment however.
Chris

Date sent: Tue, 16 Jul 2002 19:47:32 +0300
} From: Kristian Ukkonen {kristian.ukkonen-at-iki.fi}
To: microscopy-at-sparc5.microscopy.com


Hello,

We recently aquired a JSM5600LV SEM, with a Peltier cold stage. What is the
purpose of using this decive? All info on this is welcome!

Thanks

Renaat Dasseville
Protistology & Aquatic Ecology
Dept. Biology, University Gent
Krijgslaan 281, S8
9000 Gent, B E L G I U M

tel: +32 9 264 85 04
fax +32 9 264 85 99



From daemon Fri Jul 19 07:07:38 2002



From: joe.p.neilly-at-abbott.com
Date: Fri, 19 Jul 2002 06:59:54 -0500
Subject: Re: XL-30 Sirion x-ray & diffusion pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have two XL30s. One is 8 years old with a diffusion pump and one is 1 year
old with a turbo pump. Both have Edax EDS systems, and we have not seen any
evidence of contamination on the x-ray detectors. The vacuum systems on our
Philips/FEI microscopes have been very reliable.

Joe Neilly, Research Investigator
Abbott Laboratories
R45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027



Gary Gaugler
{gary-at-gaugler To: MSA listserver {Microscopy-at-sparc5.microscopy.com}
.com} cc:
Subject: XL-30 Sirion x-ray & diffusion pump
07/18/02
09:23 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anyone out there have any experience to share
about the Philips XL-30 (Sirion or other) with EDAX relative
to potential consequences of a diffusion pump versus
a turbo? It seems to me that a turbo pump would
go a long way to prevent contamination of the
detector window. I am not sure about this. The
Sirion isolates the chamber from the diffusion pump
during specimen exchange. Plus, it has a chilled
water trap. So this may not be a problem.

gg








From daemon Fri Jul 19 09:12:28 2002



From: dpu-at-spdc.ti.com (by way of Nestor J. Zaluzec)
Date: Fri, 19 Jul 2002 08:50:36 -0500
Subject: Ask-A-Microscopist: Imaging Grooves in Records

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dpu-at-spdc.ti.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on
Thursday, July 18, 2002 at 13:16:32
---------------------------------------------------------------------------

Email: dpu-at-spdc.ti.com
Name: Dennis Pu

Organization: Texas Instruments

Education: Graduate College

Location: Dallas, Texas 75243

Question: Hello,
This is not exactly a question for school,
but I hope we're all here to learn and explore.
My question deals with using microscopy to view
detailed images of the inside of vinyl record
grooves. My desire is to research what it might
take to build an optical record player.
I'm trying to determine whether it is
more practical to utilize some kind of optical
tracking of the groove for real-time play or
if scanning the whole record in at once and using
software to recognize the grooves and to produce
the sound would be better.
My first step is just to determine ways I
can use microscopy to even see inside groove. I'd
consider one economical option that hobbyists
could tinker with at home, and one "best" solution
archivists can use for transcribing old media
(vinyl, wax cylinders, acetates, etc...) without
having to use destructive mechanical means.
I'd appreciate any suggestions you have
to offer.

Thanks,
Dennis Pu

---------------------------------------------------------------------------


From daemon Fri Jul 19 09:12:28 2002



From: julycola-at-biof.ufrj.br (by way of MicroscopyListserver)
Date: Fri, 19 Jul 2002 08:48:20 -0500
Subject: Ask-A-Microscopist: 16% formaldehyde from EMS Paraformaldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (julycola-at-biof.ufrj.br) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
July 18, 2002 at 09:15:20
---------------------------------------------------------------------------

Email: julycola-at-biof.ufrj.br
Name: Juliany Rodrigues

Organization: Federal University of Rio de Janeiro

Education: Graduate College

Location: Rio de Janeiro, Brazil

Question: We are not succeeding in preparing 16% formaldehyde from
EMS Paraformaldehyde. The final mixture is turbid no matter how much
NaOH we add.
What could be the cause? We have not had this kind of trouble in many
years of preparation. Do you advise pH adjustment of the freshly
prepared solution? Thank You for the help.

---------------------------------------------------------------------------


From daemon Fri Jul 19 09:12:29 2002



From: cf_reu-at-ccmr.cornell.edu (by way of MicroscopyListserver)
Date: Fri, 19 Jul 2002 08:49:10 -0500
Subject: Ask-A-Microscopist: Etching Nickel-Titanium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cf_reu-at-ccmr.cornell.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
July 18, 2002 at 10:00:16
---------------------------------------------------------------------------

Email: cf_reu-at-ccmr.cornell.edu
Name: Chris Fanning

Organization: Cornell University

Education: Undergraduate College

Location: Ithaca, Ny, US

Question: I am involved in undergraduate materials research on
Nickel-Titanium (50.5% ni). I have prepared an ~1cm cube of nitinol
potted in epoxy to be viewed in an SEM. Our goal is to obtain an ODF
via an EBSD scan. to accomplish this we need to get well defined
patterns in the EBSD. We are getting patterns now but they are not
good enough. We are considering etching and need recomendations for
etching solutions. Many people suggest an HF solution. Are there
alternatives to HF?

Our material prep was, ...
-cut with a slow speed (200 rpm) precision saw, diamond blade
-rough polish with 240, 320, 480(?), 600, 1200 Si-carbide paper by hand
-fine polish with 9u, 3u, 1u on nylon paper - polishing wheel, 200rpm
-fine polish with colloidal silica, by hand
-4 hrs on vibraory polisher with colloidal silica

any advice would be much appreciated! sorry for the long email,

Sincerely,
Chris Fanning

---------------------------------------------------------------------------


From daemon Fri Jul 19 09:12:30 2002



From: Jason Patton :      jason.patton-at-anu.edu.au
Date: Sat, 20 Jul 2002 00:07:29 +1000
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subscribe microscopy



From daemon Fri Jul 19 09:21:17 2002



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Fri, 19 Jul 2002 10:16:08 -0600
Subject: Re: TEM-HEPES buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sergey,

I disagree with your comment that a buffer has to be used in
the 10-20 mM range, and that at 0.1 M it should be considered
a stock solution. Clearly the concentration has to be proportional
to the buffering capacity required. When using large concentrations
of PFA (4-8 %), high concentration of HEPES is required to maintain
the pH neutral. We and others use routinely 250 mM Hepes "buffer"
with PFA fixation and get great morphology (as defined by an
absence of swelling of internal membranes and lack of extraction
of cytoplasmic components). Similarly, I know of many EM groups
who use routinely 100 or 200 mM phosphate solutions for fixation
for both GA and PFA fixation. As Fred said, what matters is to try
various techniques and identify the optimal conditions. Of course,
what someone calls great fixation can be seen as poor fixation
by someone else. Since nobody really knows what a cell is
supposed to look like at the microscopical level without having to
kill it first, remove all its water content and saturate it with heavy
metals, discussing what is a good or poor morphology can be
a futile exercise. According to what I consider reasonnable criteria
(some of which are listed above), I and others have found that
fixation in 200 or 250 mM solutions of HEPES, Pipes or phosphate
actually works well.
Best regards


Marc



Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Not at all Fred. I was commenting the original message, not yours. No
} worry. I think, we both agree that in order to get good answer, we have to
} ask good questions. For instance the definition of the 'HEPES buffer' to
} me mean that people talking about 10-20 mM concentration of HEPES. 0.1 M
} HEPES is a stock solution to me. Others may think differently. As a result
} there is misunderstanding may occur (exactly what you mean). Sergey
}
} At 01:29 PM 7/18/02, you wrote:
} } Did I say something wrong? I tried not to. But I can tell from what you
} } wrote that we agree. My response was my nasty way to say that there was
} } insufficient information to draw any conclusion and frame any answer.
} }
} } Hope you agree.
} }
} } Fred
} }
} }
} } } ----------
} } } From: Sergey Ryazantsev
} } } Sent: Thursday, July 18, 2002 3:02 PM
} } } To: Monson, Frederick C.; microscopy-at-sparc5.microscopy.com
} } } Subject: RE: TEM-HEPES buffer
} } }
} } } I was surprised to find that some people in EM Labs used 0.1 M phosphate
} } } buffer as a "PBS". From my course of biochemistry/cell biology PBS
} } } stands
} } } for Phosphate Buffered Saline and is 20 mM phosphate buffer, pH 7.4 and
} } } 150
} } } mM NaCl. If you replace 20 mM phosphate on 20 mM of any other buffer,
} } } HEPES for instance, it's very unlikely the total osmomolarity will
} } } changed... BUT, if you are using 0.1 (?) M HEPES it may be a HUGE
} } } DIFFERENCE... You have to understand clearly, which buffer system you are
} } }
} } } using. In biochemistry, we never ever use 100 mM range buffers itself
} } } (like
} } } 0.1 M phosphate/HEPES/MOPS etc). The purpose of the 'buffer' is to
} } } provide
} } } necessary pH and sodium or potassium salts were used to provide necessary
} } } osmosity... GA itself affect osmosity seriously and formally speaking,
} } } osmosity should be corrected. From the 'chemical' point of view, HEPES
} } } should not affect GA efficiency. Moreover, HEPES is the best buffer for
} } } GA
} } } crosslinking because of it's huge buffer capacity in the neutral pH range.
} } }
} } } As I understand, some 'buffer systems' like cacodylate helps to wash out
} } } some content of the cell matrix and therefore makes some structural
} } } details
} } } more pronounced. Mistakenly, some people called it 'good fixation'. If
} } } you
} } } are using 100 mM range buffers, such 'washing effect' supposed to be very
} } } pronounced. HEPES is good to preserve the matrix integrity, not for
} } } washing out it's components. I am sorry for such obvious comment. Best
} } } wishes, Sergey.
} } }
} } } At 09:44 AM 7/18/02 -0400, you wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Morning,
} } } }
} } } } When you changed your buffer from the old(?) to HEPES, what
} } } change,
} } } } if any, did you record in its osmolarity? Also, there ARE non-trivial
} } } } effects recorded for different buffering systems on various tissues.
} } } Every
} } } } change should be followed by an experiment to define new optima for the
} } } } tissues under consideration, and the best way to determine new optima is
} } } to
} } } } start with semi-thin section comparisons followed by ultrastructural
} } } } analysis.
} } } }
} } } } If it was easy, we'd all be paid much more for our work.
} } } }
} } } } Regards,
} } } }
} } } }
} } } } Fred Monson
} } } }
} } } } Frederick C. Monson, PhD
} } } } Center for Advanced Scientific Imaging
} } } } Schmucker II Science Center
} } } } West Chester University
} } } } South Church Street and Rosedale
} } } } West Chester, Pennsylvania, USA, 19383
} } } } Phone: 610-738-0437
} } } } FAX: 610-738-0437
} } } } fmonson-at-wcupa.edu
} } } } CASI URL: http://darwin.wcupa.edu/casi/
} } } } WCUPA URL: http://www.wcupa.edu/
} } } } Visitors URL: http://www.wcupa.edu/_visitors/
} } } }
} } } }
} } } } } ----------
} } } } } From: Dorota Wadowska
} } } } } Sent: Wednesday, July 17, 2002 2:54 PM
} } } } } To: microscopy-at-sparc5.microscopy.com
} } } } } Subject: TEM-hepes buffer
} } } } }
} } } } }
} } } ------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} } } -----------------------------------------------------------------------.
} } } } }
} } } } }
} } } } } Hi Everybody!
} } } } } I was wondering if any of you have used Hepes buffer for electron
} } } } } microscopy fixation. We used 2% glut in Hepes and we found
} } } } } fixation unsatisfactory. Did any of you experienced any problems
} } } } } using this system?
} } } } } Thanks
} } } } } Dorota
} } } } }
} } } } }
} } }
} } }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu

--
Marc Pypaert
Department of Cell Biology,
Center for Cell and Molecular Imaging and
Ludwig Institute for Cancer Research,
Yale University School of Medicine
333 Cedar Street
New Haven CT 06520
Tel : (203) 785 3681
Fax : (203) 785 7446




From daemon Fri Jul 19 09:25:37 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Fri, 19 Jul 2002 10:19:04 -0400
Subject: antibodies for porcine sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Renaat
The purpose of the peltier cold stage is to
cool the specimen, thereby reducing the vapour pressure of any
volatile liquids, such as water, oils, that it contains. At the
temperatures attainable with a peltier module (perhaps -30oC?) water
or ice cannot be completely stabilized, unless you can match the
partial pressure of water vapour in the chamber atmosphere to the
vapour pressure above ice at the specimen temperature employed. This
may require ESEM and may not be possible using LVSEM. However, the
rate of outgassing of wet specimens may be greatly reduced.
You can also stabilize some low melting point materials, such as
waxes, chocolate or butterfat in food samples by reducing their
temperature.
Chris

} From: "Renaat Dasseville" {renaat.dasseville-at-rug.ac.be}
To: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}


Kathy:

Try the SEROTEC company at www.serotec.co.uk or Veterinary Medical Research
& Development at www.vmrd.com or Upstate Cell Signaling Solutions at
www.upstate.com

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954


-----Original Message-----
} From: kwalters [mailto:kwalters-at-blue.weeg.uiowa.edu]
Sent: Thursday, July 18, 2002 4:47 PM
To: microscopy


Hi All,

I have a project requiring that I identify cells grown in culture that were
originally taken from porcine tissue. The cells are suspected to be either
smooth muscle cells, endothelium, or fibroblasts. I am unfamiliar with
which
antibodies would work for these cells. There are many antibodies for these
types of cells, but will they work in porcine tissue? Help.....


Thanks,
Kathy

Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters-at-uiowa.edu |__| / /
|| / /
--------------
------------------



From daemon Fri Jul 19 09:45:33 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 19 Jul 2002 16:37:33 +0200
Subject: Re: XL-30 Sirion x-ray & diffusion pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't have experience of the XL30, but so far I know, the XL30 serie
uses Edwards Diffstack diff pumps, which are very good pumps, and don't
backstream a lot. We use it (with Santovac oil) on UHV surface science
instruments without any contamination probleme (and Auger is sensitiv to
carbon...). As we know, contamination comes from the rouhing pump, and a
turbo pump stops better oil from it than a diff one. The question is : how
is the SEM chamber pumped from air pressure down to 1E-2mbar ? Is
the diff pump by pased by an oil seeled rouhing pump ? If so, you will
have a source of contamination independently from the diff or turbo
pump.

To avoid all sources of contamination, you have two designs possible :
-a turbo pump backed by a scroll. The two pumps are stopped when
the specimen chamber is vented, and are put on together to pumpd down
again. I think Leo does it this way.
-a fast entry lock, pumped by a scroll pump, and idealy a turbo
and a scroll on the chamber, or less expensive, a diff and a rotary
vane pump (with alumina foreline trap). I would prefer this design,
because I don't thing it's a good idee for a HR-SEM to put the whole
chamber to air at each specimen exchange.

What is meaningsless is to put a turbo pump as secondary pump, to avoid
contamination and than use a oil sealed pump to pump down the entry lock,
or do the primary vaccum with it, by passing the turbo. Of coarse you can
put a turbo on the fast entry lock too !

In all cases, if you use a rotary vane pump, put a foreline trap on it.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Thu, 18 Jul 2002, Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone out there have any experience to share
} about the Philips XL-30 (Sirion or other) with EDAX relative
} to potential consequences of a diffusion pump versus
} a turbo? It seems to me that a turbo pump would
} go a long way to prevent contamination of the
} detector window. I am not sure about this. The
} Sirion isolates the chamber from the diffusion pump
} during specimen exchange. Plus, it has a chilled
} water trap. So this may not be a problem.
}
} gg
}
}



From daemon Fri Jul 19 10:01:21 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 19 Jul 2002 15:54:52 +0100 (GMT Daylight Time)
Subject: Re: SEM Peltier cold stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is for use in low vacuum mode. When you use it to cool
your hydrated samples they will lose water more slowly.

Dave


On Fri, 19 Jul 2002 13:36:07 +0200 Renaat Dasseville
{renaat.dasseville-at-rug.ac.be} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} We recently aquired a JSM5600LV SEM, with a Peltier cold stage. What is the
} purpose of using this decive? All info on this is welcome!
}
} Thanks
}
} Renaat Dasseville
} Protistology & Aquatic Ecology
} Dept. Biology, University Gent
} Krijgslaan 281, S8
} 9000 Gent, B E L G I U M
}
} tel: +32 9 264 85 04
} fax +32 9 264 85 99
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Jul 19 11:44:11 2002



From: Sophie Dahan :      sdahan-at-caprion.com
Date: Fri, 19 Jul 2002 12:35:19 -0400
Subject: Immunofluorescence labeling of liver and lung paraffin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We are trying to conduct immunofluorescent labeling of tissue arrays of paraffin sections of multiple tissue types (from Abcam).....we are having problems reducing the background fluorescence in some tissues....liver and lung to name just a few. These are blaring green with FITC secondary antibodies alone!
Would anyone have good (low background noise) protocols for labeling these difficult tissue types?

I thank you in advance for any advice we can get on this.
Sophie

Sophie Dahan, Ph.D.
Senior Scientist, Microscopy Lab
Caprion Pharmaceuticals, Inc.
Montreal, Quebec
Canada



From daemon Fri Jul 19 12:12:39 2002



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Fri, 19 Jul 2002 10:02:04 -0700
Subject: Workshop announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Workshop Announcement

Microscopy and Digital Imaging: Advances and Applications.
An afternoon with Dr. Alan Hibbs, Biocon, Ltd., Melbourne, Australia
Thursday, August 1, 2002, Health Sciences Building Room G-417

1:30-2:15 Medical diagnosis using miniaturized confocal microscopes and
a review of current confocal instruments for research.

2:30-3:15 Culture on the stage - growing cells and tissues on the
microscope for live cell imaging.

3:30-4:30 Refreshments and a roundtable discussion of audience questions
regarding microscopy, confocal microscopy, live cell imaging or related
issues: with the audience, Dr. Hibbs, invited UW faculty and staff.

Dr. Hibbs provides training and consulting throughout the world for
confocal microscopy and live cell microscopy.

Presented by:
The University of Washington Core for Communication Research - V.M.
Bloedel Hearing Research Center; Cellular Imaging Facility - Center for
Human Development and Disability; W.M. Keck Center for Advanced Studies
in Neural Signaling.

Refreshments are provided courtesy of Intelligent Imaging Innovations,
Denver, CO
www.intelligent-imaging.com

Please contact Glen MacDonald for more information.
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************


From daemon Fri Jul 19 14:54:17 2002



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Fri, 19 Jul 2002 15:45:40 -0400 (EDT)
Subject: TEM sperm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

Many thanks for the many very creative approaches to preparing minute
quantities of sperm for TEM. I'll pass them along to the investigator and
give some of them a try.

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu



From daemon Fri Jul 19 16:33:48 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Fri, 19 Jul 2002 12:48:27 -0400
Subject: Jeol 100C TEM for donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear TEM Community,
We have a Jeol 100C 100 KV TEM serial # EM156009-05, that we are
willing to donate to a nonprofit or educational institution. The instrument
was built in 1974 and is in fair condition. Scope has 3 sets of 50 plate
film cassettes and boxes with built in vacuum film desiccator. It has been
under service contract with Jeol for the last 10 years. We will be
dismantling the scope at the end of this month It will be the donee's
responsibility to come and take the microscope. Interested parties should
send statement of purpose and reasons why we should select them as the donee
for this microscope. Please contact


Joe Goodhouse
Confocal / EM Core Laboratory Manager
Department of Molecular Biology
Princeton University
jgoodhouse-at-molbio.princeton.edu
609-258-5432


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Fri Jul 19 16:58:26 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 19 Jul 2002 17:56:48 -0400
Subject: Ask-A-Microscopist: Etching Nickel-Titanium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris;

Try contacting Acton Technologies in Pennsylvania, USA, "www.actontech.com."
I didn't see an etch specfically for Ni-Ti but they may offer some
solutions. They make up custom mixtures as well as off-the-shelf products.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: cf_reu-at-ccmr.cornell.edu [mailto:cf_reu-at-ccmr.cornell.edu]
Sent: Friday, July 19, 2002 9:49 AM
To: microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cf_reu-at-ccmr.cornell.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
July 18, 2002 at 10:00:16
---------------------------------------------------------------------------

Email: cf_reu-at-ccmr.cornell.edu
Name: Chris Fanning

Organization: Cornell University

Education: Undergraduate College

Location: Ithaca, Ny, US

Question: I am involved in undergraduate materials research on
Nickel-Titanium (50.5% ni). I have prepared an ~1cm cube of nitinol
potted in epoxy to be viewed in an SEM. Our goal is to obtain an ODF
via an EBSD scan. to accomplish this we need to get well defined
patterns in the EBSD. We are getting patterns now but they are not
good enough. We are considering etching and need recomendations for
etching solutions. Many people suggest an HF solution. Are there
alternatives to HF?

Our material prep was, ...
-cut with a slow speed (200 rpm) precision saw, diamond blade
-rough polish with 240, 320, 480(?), 600, 1200 Si-carbide paper by hand
-fine polish with 9u, 3u, 1u on nylon paper - polishing wheel, 200rpm
-fine polish with colloidal silica, by hand
-4 hrs on vibraory polisher with colloidal silica

any advice would be much appreciated! sorry for the long email,

Sincerely,
Chris Fanning

---------------------------------------------------------------------------


From daemon Fri Jul 19 17:30:01 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 19 Jul 2002 15:21:14 -0400
Subject: Re: Ask-A-Microscopist: Imaging Grooves in Records

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Email: dpu-at-spdc.ti.com
} Name: Dennis Pu
}
} Organization: Texas Instruments
}
} Education: Graduate College
}
} Location: Dallas, Texas 75243
}
} Question: Hello,
} This is not exactly a question for school,
} but I hope we're all here to learn and explore.
} My question deals with using microscopy to view
} detailed images of the inside of vinyl record
} grooves. My desire is to research what it might
} take to build an optical record player.
} I'm trying to determine whether it is
} more practical to utilize some kind of optical
} tracking of the groove for real-time play or
} if scanning the whole record in at once and using
} software to recognize the grooves and to produce
} the sound would be better.
} My first step is just to determine ways I
} can use microscopy to even see inside groove. I'd
} consider one economical option that hobbyists
} could tinker with at home, and one "best" solution
} archivists can use for transcribing old media
} (vinyl, wax cylinders, acetates, etc...) without
} having to use destructive mechanical means.
} I'd appreciate any suggestions you have
} to offer.
}
} Thanks,
} Dennis Pu
}
} ---------------------------------------------------------------------------
}
Dear Dennis,
One problem you'll have is that vinyl doesn't reflect very well, so it
will be hard to use optical microscopy to see the groove shape. Also, the
contrast will be inherently low. Trying to get the 3-D shape of both sides
of the groove will be a lot harder than determining this mechanically with a
macroscopic version of the atomic-force microscope--aka stylus and
cartridge. It might be possible to coat the grooves with a very thin layer
of a reflective metal and get a stereo view; a CD reader must do something
like this, but the information is probably stored in a different manner to
facilitate optical reading. Good luck.
Yours,
Bill Tivol



From daemon Fri Jul 19 18:47:32 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 19 Jul 2002 19:36:37 -0400
Subject: Ask-A-Microscopist: Imaging Grooves in Records

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dennis,

I collect and restore wind up phonographs. I remember reading
about a project where someone scanned Edison wax cylinder
records with a laser(s) and created recordings that contained
more sounds than could be heard through playing the records
on the original phonographs. The information got recorded by
the original mechanical means, but could not be heard through
mechanical playing. They may have done it with early 78's,
also, but I can't remember. It was a long time ago. FYI, the
cylinder records are "vertically cut", as opposed to "horizontally
cut" like the 78's and the later vinyl records.

Anyway, you might try searching for the information under phonos,
records, etc.

Regards,
Darrell

dpu-at-spdc.ti.com (by way of Nestor J. Zaluzec) on 07/19/2002 09:50:36 AM

To: microscopy-at-sparc5.microscopy.com
cc:


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dpu-at-spdc.ti.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on
Thursday, July 18, 2002 at 13:16:32
---------------------------------------------------------------------------

Email: dpu-at-spdc.ti.com
Name: Dennis Pu

Organization: Texas Instruments

Education: Graduate College

Location: Dallas, Texas 75243

Question: Hello,
This is not exactly a question for school,
but I hope we're all here to learn and explore.
My question deals with using microscopy to view
detailed images of the inside of vinyl record
grooves. My desire is to research what it might
take to build an optical record player.
I'm trying to determine whether it is
more practical to utilize some kind of optical
tracking of the groove for real-time play or
if scanning the whole record in at once and using
software to recognize the grooves and to produce
the sound would be better.
My first step is just to determine ways I
can use microscopy to even see inside groove. I'd
consider one economical option that hobbyists
could tinker with at home, and one "best" solution
archivists can use for transcribing old media
(vinyl, wax cylinders, acetates, etc...) without
having to use destructive mechanical means.
I'd appreciate any suggestions you have
to offer.

Thanks,
Dennis Pu

---------------------------------------------------------------------------







From daemon Fri Jul 19 18:47:32 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 19 Jul 2002 19:40:44 -0400
Subject: Ask-A-Microscopist: Imaging Grooves in Records

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Denis;

An interesting assignment. However, you should be aware that the grooves
are an analog of the frequency and magnitude of the sounds recorded. If you
image the grooves of a record, say on an electron microscope, you will
notice that it not only has waves horizontally, but waves in the "Z" axis as
well [vertically]. The two surfaces represent the left and right channels
of a stereo recording, and are thus separated.

Your assignment seems to deal with a non-destructive method of getting that
analog data off the surface by some means of "imaging." Electron
microscopy, it seems to me, may image a small area, but generating "numbers"
from that surface will be daunting. And it's numbers you need, not another
analog representation. You may look into "ultrasonic" imaging, at least for
the Z axis, something that works with "Time-of-flight" like radar, but
acoustically. I have an instrument in the laboratory called a "Sonoscan"
made by Sonoscan Corp. that can generate images of surfaces
non-destructively. There is also AFM, [Atomic Force Microscope] but the
scales you need are much more gross. You don't need to measure angstroms,
but probably 10's of microns. One pass of a record needle on that vinyl and
you've just milled off many angstroms. One question you will need to answer
is what the dynamic range of those grooves are from peak to trough so that
you can faithfully reconstruct the sound range in magnitude and the other
question is the rate of change in the grooves per linear measure to
reconstruct the frequency or pitch of the sound.

If you'd like, I would be more than happy to take a piece of vinyl record
and image it in our SEM to give you some notion of the surface you are
dealing with. However, you may have to supply the record, or at least a
piece of it. My wife will not like me sawing up her Frank Sinatra records
for such lofty scientific endeavors, even if it's for a student.

Good luck and publish your results.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: dpu-at-spdc.ti.com [mailto:dpu-at-spdc.ti.com]
Sent: Friday, July 19, 2002 9:51 AM
To: microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dpu-at-spdc.ti.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on
Thursday, July 18, 2002 at 13:16:32
---------------------------------------------------------------------------

Email: dpu-at-spdc.ti.com
Name: Dennis Pu

Organization: Texas Instruments

Education: Graduate College

Location: Dallas, Texas 75243

Question: Hello,
This is not exactly a question for school,
but I hope we're all here to learn and explore.
My question deals with using microscopy to view
detailed images of the inside of vinyl record
grooves. My desire is to research what it might
take to build an optical record player.
I'm trying to determine whether it is
more practical to utilize some kind of optical
tracking of the groove for real-time play or
if scanning the whole record in at once and using
software to recognize the grooves and to produce
the sound would be better.
My first step is just to determine ways I
can use microscopy to even see inside groove. I'd
consider one economical option that hobbyists
could tinker with at home, and one "best" solution
archivists can use for transcribing old media
(vinyl, wax cylinders, acetates, etc...) without
having to use destructive mechanical means.
I'd appreciate any suggestions you have
to offer.

Thanks,
Dennis Pu

---------------------------------------------------------------------------


From daemon Sat Jul 20 02:33:48 2002



From: drands-at-avuhsd.k12.ca.us (by way of MicroscopyListserver)
Date: Sat, 20 Jul 2002 02:16:38 -0500
Subject: Ask-A-Microscopist: paraffin wax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (drands-at-avuhsd.k12.ca.us) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July
19, 2002 at 22:18:04
---------------------------------------------------------------------------

Email: drands-at-avuhsd.k12.ca.us
Name: Dave Rands

Organization: Lancaster High School/Special Education Department

Education: 9-12th Grade High School

Location: Lancaster, California, Los Angeles County

Question: We are having a difficult time getting our paraffin wax to
fill in around our speciman. We have tried several things and have
asked the other science teachers with no success. Can you help?

---------------------------------------------------------------------------


From daemon Sat Jul 20 02:33:48 2002



From: Stephen Black :      black-at-mshri.on.ca (by way of MicroscopyListserver)
Date: Sat, 20 Jul 2002 02:09:46 -0500
Subject: GFP emission lighting system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,
I read the item about a hand-held system for GFP emission. Our lab has been
using a number of lighting systems for GFP, YFP and RFP that are
manufactured in Hungary by BLS ( http://www.bls-ltd.com/ ).
Some results can be seen at ( http://www.mshri.on.ca/nagy ).Mr. Lajos Laszlo
has many years of experience in the development of biological equipment.
Regards,
Stephen Black


From daemon Sat Jul 20 02:33:48 2002



From: nuruh-at-uccmail.co.tz (by way of Nestor J. Zaluzec)
Date: Sat, 20 Jul 2002 02:10:45 -0500
Subject: Ask-A-Microscopist:indexing Diffraction Patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (nuruh-at-uccmail.co.tz) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July
19, 2002 at 09:54:16
---------------------------------------------------------------------------

Email: nuruh-at-uccmail.co.tz
Name: Saleh R

Organization: university of dar es salaam

Education: Graduate College

Location: Dar es salaam, Tanzania

Question: I want to index some spots of an electron diffraction
patterns, which web sites and/or books can help me on this?

---------------------------------------------------------------------------


From daemon Sat Jul 20 02:33:48 2002



From: bjbecker-at-ucsd.edu (by way of MicroscopyListserver)
Date: Sat, 20 Jul 2002 02:15:54 -0500
Subject: Ask-A-Microscopist: larval mussels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (bjbecker-at-ucsd.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July
19, 2002 at 20:01:15
---------------------------------------------------------------------------

Email: bjbecker-at-ucsd.edu
Name: Bonnie Becker

Organization: Scripps Institution of Oceanography

Education: Graduate College

Location: San Diego, CA USA

Question: I work with larval mussels (on the order of 100 microns),
using laser ablation technology to analyze their chemical structures.
I would like to stick them on a microscope slide such that

1. They don't pop off
2. I can put them on the slide in water and let the water dissolve
without handling them individually.
3. If possible (doubt it), it should be trace metal free.

So, I am looking for a coated microscope slide, but I don't know if I
need poly-l-lysine, silane, or something else? The shells are CaCO3,
and they are on the order of 100 microns.

Thanks!

--

---------------------------------------------------------------------------


From daemon Sat Jul 20 21:27:33 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Sat, 20 Jul 2002 16:09:04 -1000 (HST)
Subject: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Schroedinger's cat aside, some ethical and legal issues about scientific
images will be raised at M&M 2002 in Quebec.

First, at the Presidential Happenings symposium on Monday at 5:00, Colin
Smith of Adobe Systems, Inc. in Canada will present "Harnessing the Power
of Photoshop 7", demonstrating the new and powerful features of this
latest release of Adobe Photoshop. Those of you who saw Julianne Kost's
excellent presentation at M&M 2001 in Long Beach will remember the
audience gasping at both the wonderful features of Photoshop 6 they may
not have known about, and also at the power to potentially manipulate
image data beyond all recognition.

On Tuesday the Problem Solving with the Experts program, beginning at 8:00
am, will be Addressing Issues in Digital Imaging for the
Microscopist: II. In addition to Jose Mascorro speaking about converting
film negatives to digital files and Judy Murphy explaining her solution
for handling huge numbers of digital files in the microscopy laboratory, I
will be presenting a talk, "Adobe Photoshop for Image Adjustment: How to
Start and When to Stop". For the first part I will demonstrate the usual
steps required to prepare digital micrographs and combine them into a
figure plate for publication. Then I hope to stimulate a discussion on the
kinds of manipulation that can be performed on images and the effects
those may have on data, and what this might mean for truth and ethics in
scientific imaging.

On Thursday, beginning at 10:30 am, the Technologists' Forum Roundtable
Discussion will be about the Legal and Ethical Issues of Data Ownership,
focusing on copyrights of images and other forms of data. Barbara
M. Knoppers, a recognized expert in ethics and the law, and other
representatives of academia and industry will be on the panel.

In addition, there may also be a Public Policy Committee session on
Wednesday at 2:00 pm about the copyright and legal issues, so watch the
program and daily newsletter for an announcement.

The MSA Education Committee has recently added a subcommittee on the
Ethics of Digital Image Processing, with the purpose of drafting a white
paper of recommendations.

As you can see, there are lots of opportunities for lively discussion!

See you all in Quebec,
Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Sun Jul 21 17:41:38 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Sun, 21 Jul 2002 17:13:56 -0500
Subject: facility support survey at last

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
Here finally are the results of the facility support survey first put
out at last year's M&M meeting, then on the microscopy and confocal
listservers.
Alltheusualexcuses for the delay. Including formatting the results in
ascii for the email servers.
I did try to think of wise things to say about this, but any comments
would take far too much space for the listservers, so I've decided to
simply present the raw numbers. I hope there will be room to run
these results in Microscopy Today as graphs or something, but I can
make no promises.
%s of course don't add to 100 because of rounding errors.

The analyses of the returned surveys were done by Barbara Foster's
crew MME ( mme-at-mail.map.com ). Many thanks, Barbara and gang.

There is no heading #1 as that contained information that would
identify individual institutions. I discarded that information before
I sent the surveys to MME or indeed did anything else. This
information was collected solely in case of duplicate responses, and
was trashed as soon as it was no longer needed for this purpose.

There is the usual problem with sample size. I hope the information
will be useful in spite of this.
I also hope some vestige of legible organization survives the email process ...
Phil

2. Nature of institution
COUNT %
Academic 50 89
Other 4 7
Private research 2 4
Commercial 1 2
56 Total Respondents
57 Total Responses

3. Type of facility
COUNT %
Institution core 32 58
Departmental 19 35
Other 5 9
Satellite 1 2
55 Total Respondents
57 Total Responses
4. Predominate work
COUNT %
Biological 53 87
Materials 27 44
61 Total Respondents
80 Total Responses

5. User Base
COUNT %
Multi - user 56 93
Service 47 78
60 Total Respondents
103 Total Responses

6. Type of microscopes
COUNT %
TEM 48 80
SEM 36 60
Optical 18 30
Confocal 18 30
Other 18 30
Other Optical 16 27
FESEM 12 20
ESEM or LV 12 20
Other Confocal 5 8
Scanning Probe 5 8
FETEM 3 5
Other Scanning Probe 3 5
60 Total Respondents
194 Total Responses

7. Salaries
COUNT %
100% Your Institution 31 50
80% Your Institution 6 10
50% Your Institution 5 8
100% User fees 4 6
20% Grants 4 6
100% Grants 4 6
10% Your Institution 3 5
20% User fees 3 5
25% User fees 3 5
30% User fees 3 5
50% User fees 3 5
25% Grants 3 5
70% Your Institution 2 3
75% Your Institution 2 3
10% User fees 2 3
10% Grants 2 3
50% Grants 2 3
Other 2 3
20% Your Institution 1 2
30% Your Institution 1 2
60% Your Institution 1 2
90% Your Institution 1 2
Other 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
Other User fee percentage 1 2
30% Grants 1 2
40% Grants 1 2
70% Grants 1 2
75% Grants 1 2
Other Grants 1 2
62 Total Respondents
98 Total Responses

8. Instrument maintenance
COUNT %
100% User fees 19 31
100% Your Institution 18 30
50% Your Institution 6 9
50% Grants 4 6
10% Your Institution 3 5
90% Your Institution 3 5
50% User fees 3 5
60% User fees 3 5
20% Your Institution 2 3
40% Your Institution 2 3
Other 2 3
10% User fees 2 3
80% User fees 2 3
90% User fees 2 3
10% Grants 2 3
20% Grants 2 3
100% Grants 2 3
25% Your Institution 1 2
30% Your Institution 1 2
25% User fees 1 2
30% User fees 1 2
60% Grants 1 2
75% Grants 1 2
Other 1 2
61 Total Respondents
84 Total Responses

9. Replacement of existing equipment
COUNT %
100% Grants 16 30
100% Your Institution 11 20
50% Your Institution 6 11
50% Grants 5 9
60% Grants 5 9
20% Your Institution 4 7
30% Your Institution 4 7
10% User fees 4 7
100% User fees 4 7
25% Your Institution 3 5
40% Your Institution 3 5
20% User fees 3 5
50% User fees 3 5
10% Your Institution 2 4
70% Grants 2 4
75% Grants 2 4
90% Grants 2 4
40% User fees 1 2
75% User fees 1 2
80% User fees 1 2
90% User fees 1 2
10% Grants 1 2
40% Grants 1 2
80% Grants 1 2
Other 1 2
56 Total Respondents
87 Total Responses

10. Purchasing of new equipment
COUNT %
100% Grants 21 37
100% Your Institution 9 16
50% Your Institution 6 11
50% Grants 6 11
20% Your Institution 5 9
30% Your Institution 5 9
70% Grants 4 7
20% User fees 3 5
60% Grants 3 5
80% Grants 3 5
10% Your Institution 2 4
25% Your Institution 2 4
40% Your Institution 2 4
75% Your Institution 2 4
10% User fees 2 4
30% User fees 2 4
100% User fees 2 4
10% Grants 2 4
90% Grants 2 4
60% Your Institution 1 2
70% Your Institution 1 2
40% User fees 1 2
25% Grants 1 2
40% Grants 1 2
75% Grants 1 2
57 Total Respondents
89 Total Responses

11. Supplies
COUNT %
100% User fees 28 46
100% Your Institution 9 15
50% Grants 5 8
80% Your Institution 4 7
10% Your Institution 3 5
50% Your Institution 3 5
50% User fees 3 5
90% User fees 3 5
10% Grants 3 5
90% Your Institution 2 3
10% User fees 2 3
30% User fees 2 3
20% Grants 2 3
100% Grants 2 3
20% Your Institution 1 2
25% Your Institution 1 2
30% Your Institution 1 2
40% Your Institution 1 2
60% Your Institution 1 2
20% User fees 1 2
25% User fees 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
40% Grants 1 2
70% Grants 1 2
90% Grants 1 2
Other 1 2
61 Total Respondents
85 Total Responses

12. Operating expenses
COUNT %
100% User fees 19 40
100% Your Institution 16 33
50% Your Institution 6 12
50% User fees 6 12
100% Grants 3 6
20% Your Institution 1 2
40% Your Institution 1 2
60% Your Institution 1 2
80% Your Institution 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
20% Grants 1 2
Other 1 2
48 Total Respondents
59 Total Responses
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Sun Jul 21 19:57:44 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 21 Jul 2002 17:53:27 -0700
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Will the transactions at the conference be available
either electronically or via print? This would be most
interesting.

I'm curious about the distinction between ethics and
legal issues. Image analysis technology can produce
legal data in cases where it was not possible in the past.
I don't see that this has anything to do with ethics. It
seems to me to be an issue of what can technology do
and then what will the legal system accept? There are numerous
instances of this issue. So far as I know, technology won.

gary g.



From daemon Sun Jul 21 23:17:42 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 21 Jul 2002 21:13:37 -0700
Subject: Amray 1830 final aperture holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have one of these assemblies and do not need it.

If someone can use it, please let me know. It only
fits the conical lens 1800-series units (1830)...not the flat
lens 1800-series units.



From daemon Mon Jul 22 04:47:01 2002



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Mon, 22 Jul 2002 10:36:37 +0100
Subject: Re: Ask-A-Microscopist: Imaging Grooves in Records

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dennis

when I saw your query, I thought I had seen such a technology mentioned
in a magazine. If you look at the websites below there are companies
which are already using laser scanning technology to read vinyl LPs.

http://www.stereotimes.com/turn030300.shtm

http://avconvert.com/laserturntable/

This would suggest that this is a mature technology but the 'kit' is
very expensive.

Malcolm Haswell


"by way of Nestor J. Zaluzec" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (dpu-at-spdc.ti.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on
} Thursday, July 18, 2002 at 13:16:32
} ---------------------------------------------------------------------------
}
} Email: dpu-at-spdc.ti.com
} Name: Dennis Pu
}
} Organization: Texas Instruments
}
} Education: Graduate College
}
} Location: Dallas, Texas 75243
}
} Question: Hello,
} This is not exactly a question for school,
} but I hope we're all here to learn and explore.
} My question deals with using microscopy to view
} detailed images of the inside of vinyl record
} grooves. My desire is to research what it might
} take to build an optical record player.
} I'm trying to determine whether it is
} more practical to utilize some kind of optical
} tracking of the groove for real-time play or
} if scanning the whole record in at once and using
} software to recognize the grooves and to produce
} the sound would be better.
} My first step is just to determine ways I
} can use microscopy to even see inside groove. I'd
} consider one economical option that hobbyists
} could tinker with at home, and one "best" solution
} archivists can use for transcribing old media
} (vinyl, wax cylinders, acetates, etc...) without
} having to use destructive mechanical means.
} I'd appreciate any suggestions you have
} to offer.
}
} Thanks,
} Dennis Pu


From daemon Mon Jul 22 05:31:27 2002



From: :      PHYSIOL4-at-sun.ac.za
Date: Mon, 22 Jul 2002 12:13:45 +0200
Subject: Re: Ask-A-Microscopist: paraffin wax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{color} {param} 0100,0100,0100 {/param} Hi there


What kind of specimens are you working with? I'm not sure what
you meant by " {/color} We are having a difficult time getting our paraffin
wax to fill in around our speciman" - do you mean the wax won't
literally surround your specimen, or do you mean the wax won't go
{italic} inside {/italic} the specimen? If you mean it won't go inside the specimen,
you can try placing your specimen, together with the wax, under a
vacuum overnight. If this still doesn't work.... maybe your tissue isn't
fixed properly?


Hope this helps! {color} {param} 0100,0100,0100 {/param}

{nofill}

"When life hands you a lemon ... bring out the tequila and salt."


From daemon Mon Jul 22 08:37:40 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 22 Jul 2002 09:27:50 -0400
Subject: RE: Immunofluorescence labeling of liver and lung paraffin sectio

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Sophie,

Nice problem. First a question. How does one contrive a microarray
solution without using micro-sections? And I'm not trying to be funny.

Found these by searching Google for {bilirubin autofluorescence}

http://info.med.yale.edu/labmed/faculty/labs/krauselab/HumanBMliver.pdf

The above has a specific reference to autofluorescense in liver.

http://info.med.yale.edu/labmed/faculty/labs/krauselab/miceliver.pdf

The above is even better (photomicrographs).

The liver is a biochemical factory with one of its functions focused on
detoxification. It's association with degradation of hemoglobin is the
source of your consternation. If you want to defeat the autofluorescence,
you will have to post-process the molecules to "quench" them chemically
without destroying any of the cellular antigens. Since the source of this
background is most likely large quantities of resonant molecules, I would
think a bath in bromine (additive) might do the trick, or a hydrogenation
(additive too but perhaps more dangerous), or a hydration, but the last is
usually hydrolytic. This starts as a problem in organic chemistry and ends
with a choice of a relatively mild alternative.

The folks at Yale seem to have some experience here, you might ask them
directly for help on the above.

I think!

Good luck,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/



} ----------
} From: Sophie Dahan
} Sent: Friday, July 19, 2002 12:35 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Immunofluorescence labeling of liver and lung paraffin
} sections
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} We are trying to conduct immunofluorescent labeling of tissue arrays of
} paraffin sections of multiple tissue types (from Abcam).....we are having
} problems reducing the background fluorescence in some tissues....liver and
} lung to name just a few. These are blaring green with FITC secondary
} antibodies alone!
} Would anyone have good (low background noise) protocols for labeling these
} difficult tissue types?
}
} I thank you in advance for any advice we can get on this.
} Sophie
}
} Sophie Dahan, Ph.D.
} Senior Scientist, Microscopy Lab
} Caprion Pharmaceuticals, Inc.
} Montreal, Quebec
} Canada
}
}
}


From daemon Mon Jul 22 09:50:58 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 22 Jul 2002 10:41:49 -0400
Subject: RE: Ask-A-Microscopist: Imaging Grooves in Records

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's the REAL dope, answering your question with a photo and a URL.

Hope it helps: http://members01.chello.se/christer.hamp/phono/poliak.html

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: dpu-at-spdc.ti.com
} Sent: Friday, July 19, 2002 9:50 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: Imaging Grooves in Records
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (dpu-at-spdc.ti.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on
} Thursday, July 18, 2002 at 13:16:32
} --------------------------------------------------------------------------
} -
}
} Email: dpu-at-spdc.ti.com
} Name: Dennis Pu
}
} Organization: Texas Instruments
}
} Education: Graduate College
}
} Location: Dallas, Texas 75243
}
} Question: Hello,
} This is not exactly a question for school,
} but I hope we're all here to learn and explore.
} My question deals with using microscopy to view
} detailed images of the inside of vinyl record
} grooves. My desire is to research what it might
} take to build an optical record player.
} I'm trying to determine whether it is
} more practical to utilize some kind of optical
} tracking of the groove for real-time play or
} if scanning the whole record in at once and using
} software to recognize the grooves and to produce
} the sound would be better.
} My first step is just to determine ways I
} can use microscopy to even see inside groove. I'd
} consider one economical option that hobbyists
} could tinker with at home, and one "best" solution
} archivists can use for transcribing old media
} (vinyl, wax cylinders, acetates, etc...) without
} having to use destructive mechanical means.
} I'd appreciate any suggestions you have
} to offer.
}
} Thanks,
} Dennis Pu
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Mon Jul 22 10:09:33 2002



From: swiding :      swiding-at-temple.edu
Date: Mon, 22 Jul 2002 11:03:09 -0400
Subject: Software hunt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List,

This is not a technical problem. I am seaching for graphics
software for creating\printing posters and banners. We have poster
sessions several times a year for the students. We were using an old
DOS program but our computer lab has been upgraded(OS + network printers)
and I can't get the program to work with the network printers.

Does anyone have any suggestions for this software application? It has to
be user friendly because of the students. And it has to be cheap because
of the budget.


Thanks,

Steve Widing
EM Tech / Computer Labs Manager
Biology Department
Temple University
Philadelphia, PA



From daemon Mon Jul 22 12:00:47 2002



From: JHoffpa464-at-aol.com
Date: Mon, 22 Jul 2002 12:54:24 -0400
Subject: Re: Ask-A-Microscopist: paraffin wax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"When life hands you a lemon ... bring out the tequila and salt."
nice thing to say to underage kids!!!!!!


From daemon Mon Jul 22 12:49:09 2002



From: Brian Matsumoto :      matsumot-at-lifesci.ucsb.edu
Date: Mon, 22 Jul 2002 10:43:02 -0700
Subject: Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Course Announcement for Microscopy Workshop, west coast
Deadline to enroll August 7, 2002

The seminar / workshop will be an intensive lecture/laboratory series that
will enable participants to develop theoretical and hands-on expertise with
light microscopes. Attendees will closely interact with the instructors
while using modern research grade microscopes, cameras, and computers.

The Integrated Microscopy Facility, operated by the Department of Molecular,
Cellular, and Developmental Biology (MCDB) and the Neuroscience Research
Institute (NRI), is offering a workshop on modern techniques in microscopy
and electronic imaging. This 5-day workshop will be offered from September
9th through September 13th, 2001 and will consist of lectures and laboratory
exercises that will run from 9 am to approximately 5 pm each day.

The seminars and laboratories will cover basic optical theory and how it
pertains to increasing contrast (signal to noise ratio) in biological
samples. Fundamental techniques such as fluorescence, phase contrast,
Nomarski differential interference contrast, and darkfield imaging will be
taught and attendees will use microscopes equipped to perform these optical
enhancement techniques. In addition, the theory and practice of electronic
image acquisition (analog and digital) will be discussed and attendees will
work with low-light cameras, digital image processing computers, and
morphometric programs. There are five research grade microscopes, five
electronic imaging cameras, three computer workstations, and one confocal
microscope. With a maximum enrollment of 10 students, there will be ample
opportunity to work with all of the microscopes and cameras. For those so
interested, intensive hands-on instruction and guidance on the confocal
microscope will be provided. Working together with the instructors, the
participants will gain theoretical and practical experience on applying
modern optical and computational techniques for biological research.

September 9th
Morning Lab and Seminar: Fundamentals of Microscopy. Parts of the
microscope, obtaining Köhler illumination and the nature of light.
Afternoon Lab and Seminar: Optical Enhancement of the Image. Phase contrast,
darkfield, and Nomarski Differential Interference Contrast.
After 6pm: Optional review and lab work.

September 10th
Morning Lab and Seminar: Fluorescence microscopy: Selection of
fluorochromes, filters and objectives.
Afternoon Lab and Seminar: Role of specimen preparation in Fluorescence
microscopy. Introduction to digital microscopy.
After 6pm: Optional review and lab work.

September 11th
Morning Lab and Seminar: Image Acquisition for Microscopy: Selection of
electronic cameras.
Afternoon Lab and Seminar: Computer Enhancement of the image: Uses of image
processing.
After 6 pm: Optional review and lab work.

September 12th
Morning Lab and Seminar: Confocal Microscopy and Deconvolution
Afternoon Lab and Seminar: Continuation with Confocal Microscopy and 3D
imaging
After 6pm: Beach Barbecue

September 13th
Morning Lab Review
Afternoon Lab Optional Topics (attendees choice)

To find out more about the course and to register, please go to the
following web site:

http://www.lifesci.ucsb.edu/mcdb/events/imaging_workshop/index.php

===============================
Brian Matsumoto, Ph.D.
Research Biologist
Dept. MCDB & NRI
University of California
Santa Barbara, CA 93106

Office Phone 805-893-8702
FAX 805-893-4724



From daemon Mon Jul 22 12:49:10 2002



From: jinsong wu :      jinsong.wu-at-asu.edu
Date: Mon, 22 Jul 2002 10:33:20 -0700
Subject: liquid He transferring kids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Listers:

We have a new liquid-He holder. However, it takes three men to
work together for about one and half hours for every filling up of the
holder. At the same time, there is high risk to destroy the vacuum.
Then we have merely about 30 minutes' working time.

Please let me know your kind suggestions on the possible techniques
of the transferring of the liquid He from the tank to the holder.
For example, is it possible to put the He tank in near room and use
some transferring kits connecting the tank and the holder so as to be
able to work continuously?

Any replies from commercial sources or vendors are welcome to my
personal email address: jinsong.wu-at-asu.edu.

Thanks a lot,

jinsong wu
Department of Physics and Astronomy
Arizona State University
Tempe, AZ 85287-1504

Tel: 480-965-2535 (o)





From daemon Mon Jul 22 12:56:37 2002



From: Gabriel A. Rosa :      cimic-at-bg.fcen.uba.ar
Date: Mon, 22 Jul 2002 14:50:40 -0300
Subject: PhiliipsTEM 301 Spare part Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, I need a piece of the TEM Philips 301, and I need
to know if someone can donate it, or sell it, it is the
ROLL MEMBRANE of the camera plates, the serial number is 5322 360
40071

Thanks



Gabriel Adriano Rosa
Area Servicios Generales
Departamento de Ciencias Biologicas
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires
Ciudad Universitaria, 4 piso, Pab. II,
C1428EHA, Buenos Aires, ARGENTINA
TE -(54-11)-4576-3349
FAX (54-11)-4576-3384
e-mail micros-at-bg.fcen.uba.ar




From daemon Mon Jul 22 13:58:10 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 22 Jul 2002 08:50:33 -1000 (HST)
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary Gaugler wrote:

} Will the transactions at the conference be available
} either electronically or via print? This would be most
} interesting.
}
} I'm curious about the distinction between ethics and
} legal issues. Image analysis technology can produce
} legal data in cases where it was not possible in the past.
} I don't see that this has anything to do with ethics. It
} seems to me to be an issue of what can technology do
} and then what will the legal system accept? There are numerous
} instances of this issue. So far as I know, technology won.

I hope to have a transcript of these sessions available in print at some
point.

One thing I hope we can do is make the distinction between manipulation of
"native" images (the definition of which is also a question) for
publication as a representation of true image, and "image analysis" for
ferreting out information from these images.

A few organizations have guidelines for image manipulation:

1. The criminal justice system has a Scientific Working Group on Imaging
Technologies (SWGIT) that has come up with recommendations for capture,
storage, processing, analysis, transmission and output of
images. http://www.for-swg.org/it_files/swgit_guidelines.html

2. The National Press Photographers Association (NPPA) has a general code
of ethics and a statement specifically about digital media at
http://www.nppa.org/services/bizpract/digitalethics.html See also the
DigitalCustom Model Ethics Guidelines at
http://www.digitalcustom.com/howto/mediaguidelines.htm for suggested
guidelines for journalists.

3. The Department of Defense once had a Memorandum on Digital Manipulation
that appeared in the Military Review. Although I have not managed to get
my hands on the original, it is reproduced in part at
http://media.gn.apc.org/manidod.html

4. Other organizations, such as the North American Nature Photographers
Association (NANPA) have ethics committees that are trying to formulate
guidelines, or at least a way to label what sorts of manipulations have
been performed on images. See a couple of papers and discussions at
http://www.nanpa.org/committees/ethics/manip_intro.html

In addition, several agencies, such as the FDA, have guidelines for the
handling, transmission and storage of images, especially those potentially
involved in legal issues. In those cases, each step in acquiring,
storing, moving and manipulation of images must be documented, as well as
a record of anyone who has accessed those files.

Generally, these organizations have had to come up with guidelines for
images that may be used in legal proceedings. However, research scientists
generally operate in a self-policing environment, where a code of ethics
encourages presentation of the "truth". All kinds of data can be
manipulated in many ways, either fraudently or innocently. It's the
innocent/uninformed/untrained use of digital image manipulation that
scares me the most!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Mon Jul 22 15:53:13 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 22 Jul 2002 15:45:27 -0500
Subject: support survey again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It spite of my best efforts to format the survey with Geneva
fixed-width font in ascii, it still came across to some folks (many?)
all disarranged. So Here is the survey reformatted by J Quinn at SUNY
(Stony Brook, I believe).
Phil
**************************************************

2. Nature of institution
COUNT %
Academic 50 89
Other 4 7
Private research 2 4
Commercial 1 2

56 Total Respondents
57 Total Responses

**************************************************

3. Type of facility
COUNT %
Institution core 32 58
Departmental 19 35
Other 5 9
Satellite 1 2

55 Total Respondents
57 Total Responses

**************************************************

4. Predominate work
COUNT %
Biological 53 87
Materials 27 44

61 Total Respondents
80 Total Responses

**************************************************

5. User Base
COUNT %
Multi - user 56 93
Service 47 78

60 Total Respondents
103 Total Responses

**************************************************

6. Type of microscopes
COUNT %
TEM 48 80
SEM 36 60
Optical 18 30
Confocal 18 30
Other 18 30
Other Optical 16 27
FESEM 12 20
ESEM or LV 12 20
Other Confocal 5 8
Scanning Probe 5 8
FETEM 3 5
Other Scanning Probe 3 5

60 Total Respondents
194 Total Responses

**************************************************

7. Salaries
COUNT %
100% Your Institution 31 50
80% Your Institution 6 10
50% Your Institution 5 8
100% User fees 4 6
20% Grants 4 6
100% Grants 4 6
10% Your Institution 3 5
20% User fees 3 5
25% User fees 3 5
30% User fees 3 5
50% User fees 3 5
25% Grants 3 5
70% Your Institution 2 3
75% Your Institution 2 3
10% User fees 2 3
10% Grants 2 3
50% Grants 2 3
Other 2 3
20% Your Institution 1 2
30% Your Institution 1 2
60% Your Institution 1 2
90% Your Institution 1 2
Other 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
Other User fee percentage 1 2
30% Grants 1 2
40% Grants 1 2
70% Grants 1 2
75% Grants 1 2
Other Grants 1 2

62 Total Respondents
98 Total Responses

**************************************************

8. Instrument maintenance
COUNT %
100% User fees 19 31
100% Your Institution 18 30
50% Your Institution 6 9
50% Grants 4 6
10% Your Institution 3 5
90% Your Institution 3 5
50% User fees 3 5
60% User fees 3 5
20% Your Institution 2 3
40% Your Institution 2 3
Other 2 3
10% User fees 2 3
80% User fees 2 3
90% User fees 2 3
10% Grants 2 3
20% Grants 2 3
100% Grants 2 3
25% Your Institution 1 2
30% Your Institution 1 2
25% User fees 1 2
30% User fees 1 2
60% Grants 1 2
75% Grants 1 2
Other 1 2
61 Total Respondents
84 Total Responses

**************************************************

9. Replacement of existing equipment
COUNT %
100% Grants 16 30
100% Your Institution 11 20
50% Your Institution 6 11
50% Grants 5 9
60% Grants 5 9
20% Your Institution 4 7
30% Your Institution 4 7
10% User fees 4 7
100% User fees 4 7
25% Your Institution 3 5
40% Your Institution 3 5
20% User fees 3 5
50% User fees 3 5
10% Your Institution 2 4
70% Grants 2 4
75% Grants 2 4
90% Grants 2 4
40% User fees 1 2
75% User fees 1 2
80% User fees 1 2
90% User fees 1 2
10% Grants 1 2
40% Grants 1 2
80% Grants 1 2
Other 1 2

56 Total Respondents
87 Total Responses

**************************************************

10. Purchasing of new equipment
COUNT %
100% Grants 21 37
100% Your Institution 9 16
50% Your Institution 6 11
50% Grants 6 11
20% Your Institution 5 9
30% Your Institution 5 9
70% Grants 4 7
20% User fees 3 5
60% Grants 3 5
80% Grants 3 5
10% Your Institution 2 4
25% Your Institution 2 4
40% Your Institution 2 4
75% Your Institution 2 4
10% User fees 2 4
30% User fees 2 4
100% User fees 2 4
10% Grants 2 4
90% Grants 2 4
60% Your Institution 1 2
70% Your Institution 1 2
40% User fees 1 2
25% Grants 1 2
40% Grants 1 2
75% Grants 1 2

57 Total Respondents
89 Total Responses

**************************************************

11. Supplies
COUNT %
100% User fees 28 46
100% Your Institution 9 15
50% Grants 5 8
80% Your Institution 4 7
10% Your Institution 3 5
50% Your Institution 3 5
50% User fees 3 5
90% User fees 3 5
10% Grants 3 5
90% Your Institution 2 3
10% User fees 2 3
30% User fees 2 3
20% Grants 2 3
100% Grants 2 3
20% Your Institution 1 2
25% Your Institution 1 2
30% Your Institution 1 2
40% Your Institution 1 2
60% Your Institution 1 2
20% User fees 1 2
25% User fees 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
40% Grants 1 2
70% Grants 1 2
90% Grants 1 2
Other 1 2

61 Total Respondents
85 Total Responses

**************************************************

12. Operating expenses
COUNT %
100% User fees 19 40
100% Your Institution 16 33
50% Your Institution 6 12
50% User fees 6 12
100% Grants 3 6
20% Your Institution 1 2
40% Your Institution 1 2
60% Your Institution 1 2
80% Your Institution 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
20% Grants 1 2
Other 1 2

48 Total Respondents
59 Total Responses

**************************************************


From daemon Mon Jul 22 15:53:13 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 22 Jul 2002 16:44:47 -0400
Subject: RE: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary,

In imaging, it would be unethical for a female model to hand deliver a photo
which showed an amplified chest prior to surgery, unless she stipulated that
she is hopeful that she will look as she appears in the photo after her
surgery is done. If, on the other hand, she sent such photos thru the mail
without an accompanying declaration, she might be arrested for fraudulent
use of the mail.

It is both legal and ethical for the model to send such an amplified photo
to past and future clients and ask if she has surgery and alters her
appearance as in the photo whether she will be more or less employable.

She can be both legal and ethical with her boyfriends by sending them such a
photo and asking whether she will be more or less enjoyable after such an
alteration.

It would be illegal for the model to represent herself with an
enhanced chest in a public advertisement without the declaration. She would
also be leaving herself legally liable is she, unethically, used such a
photo to acquire employment on the pretence that she had the chest in the
photograph unless she arrived at the job with a note from her physician
attesting to the fact that between the sending of the photograph, her
employment and her arrival, her chest had suffered a deflation.

I cannot think of anything more along these lines now. I would have
to see the original and altered photographs before reaching any other
conclusions.

They may be sent to the address below.

If a male model were to act in the manner of the female described
above, let the employer and boyfriend beware, there is NO legal or ethical
issue that can save them.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/






} ----------
} From: Gary Gaugler
} Sent: Sunday, July 21, 2002 8:53 PM
} To: MSA listserver
} Subject: Re: Ethical and legal issues in imaging
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Will the transactions at the conference be available
} either electronically or via print? This would be most
} interesting.
}
} I'm curious about the distinction between ethics and
} legal issues. Image analysis technology can produce
} legal data in cases where it was not possible in the past.
} I don't see that this has anything to do with ethics. It
} seems to me to be an issue of what can technology do
} and then what will the legal system accept? There are numerous
} instances of this issue. So far as I know, technology won.
}
} gary g.
}
}
}


From daemon Mon Jul 22 16:32:58 2002



From: Carlos Kazuo Inoki :      kazuo-at-csc.albany.edu
Date: Mon, 22 Jul 2002 17:26:28 -0400 (EDT)
Subject: service center for Leitz Optical Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We have a Leitz Ortholux II optical microscope that needs to be
serviced. I tried to contact the manufacturer for the a list of service
center nearby, but the 2 companies they indicated are possibly
out-of-business (phone number not working). Can someone indicate some
service center near tho my area, Albany-NY? What we need is clean/align
the optics and fix a mechanical problem with the stage. Regards,

Carlos

o-------------------------------------------------------o
| Carlos Kazuo Inoki |
| Department of Physics - University at Albany |
| 1400 Washington Ave.- Albany - NY - 12222 |
o-------------------------------------------------------o



From daemon Mon Jul 22 16:33:39 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 22 Jul 2002 14:27:53 -0700 (PDT)
Subject: Re: Software hunt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Adobe Illustrator is the software of choice for making posters. It is
available at academic discount for educators for about $80-$150 or so.
Other option would be to buy an older used version from somone. You
should be able to get it quite cheaply.

Other software you might find economic and useful is from www.jasc.com
they have paintshop pro which is similar to photoshop, but much cheaper.


\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Mon, 22 Jul 2002, swiding wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello List,
}
} This is not a technical problem. I am seaching for graphics
} software for creating\printing posters and banners. We have poster
} sessions several times a year for the students. We were using an old
} DOS program but our computer lab has been upgraded(OS + network printers)
} and I can't get the program to work with the network printers.
}
} Does anyone have any suggestions for this software application? It has to
} be user friendly because of the students. And it has to be cheap because
} of the budget.
}
}
} Thanks,
}
} Steve Widing
} EM Tech / Computer Labs Manager
} Biology Department
} Temple University
} Philadelphia, PA
}
}



From daemon Mon Jul 22 18:13:16 2002



From: Larry Hanke :      hanke-at-mee-inc.com
Date: Mon, 22 Jul 2002 18:00:44 -0500
Subject: Re: Software hunt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suggest that you look into Paint Shop Pro from JASC
Software. This is a Photoshop-type program with a lot of
functionality and low price ~$100US. I think that the
program can be downloaded for evaluation at jasc.com. We use
this program extensively for posters, image processing,
printing, etc.
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870




From daemon Mon Jul 22 20:22:21 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Mon, 22 Jul 2002 18:12:20 -0400
Subject: Re: Software hunt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 7/22/02 11:03 AM, swiding at swiding-at-temple.edu wrote:

}
} This is not a technical problem. I am seaching for graphics
} software for creating\printing posters and banners. We have poster
} sessions several times a year for the students. We were using an old
} DOS program but our computer lab has been upgraded(OS + network printers)
} and I can't get the program to work with the network printers.
}
} Does anyone have any suggestions for this software application? It has to
} be user friendly because of the students. And it has to be cheap because
} of the budget.
}
Dear Steve,
I have used PrintMaster for this and other applications. They do not
make a version for Mac (boo, hiss) but their Windows version works well, is
not expensive, and is easy to use. Good luck. I am not affiliated with
this product or its manufacturer (not even as a user, since I now have an
iBook).
Yours,
Bill Tivol



From daemon Mon Jul 22 20:24:29 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Mon, 22 Jul 2002 18:16:19 -0400
Subject: Re: liquid He transferring kids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 7/22/02 1:33 PM, jinsong wu at jinsong.wu-at-asu.edu wrote:

} We have a new liquid-He holder. However, it takes three men to
} work together for about one and half hours for every filling up of the
} holder. At the same time, there is high risk to destroy the vacuum.
} Then we have merely about 30 minutes' working time.
}
} Please let me know your kind suggestions on the possible techniques
} of the transferring of the liquid He from the tank to the holder.
} For example, is it possible to put the He tank in near room and use
} some transferring kits connecting the tank and the holder so as to be
} able to work continuously?
}
} Any replies from commercial sources or vendors are welcome to my
} personal email address: jinsong.wu-at-asu.edu.
}
} Thanks a lot,
}
Dear Jinsong,
Could you please post a summary of the replies you get to the list
(assuming I'm not the only one interested)? TIA.
Yours,
Bill Tivol



From daemon Tue Jul 23 04:17:18 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Tue, 23 Jul 2002 11:07:37 +0200
Subject: Software for TEM in Materials Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I asked once before but didn't get many replies.

What software are people using for interpreting TEM images and diffraction
patterns?

I am here NOT including simulation of HREM or simulation of dislocation
images in CTEM.

What I am particularly interested in is simulation of Kikuchi patterns and
SAD patterns, and plotting of stereographic projections for determining
dislocation line directions.

Do the makers of Desktop Microscopist still exist? Is there a current
version of this package? What is it like? Earlier versions tended to have
rather too many bugs and a tendency for unexpected crashes. Nevertheless, it
did lots of useful stuff.

Is Ideal Microscope still for sale? This is also handy for some things.

It doesn't have to be for Mac, it could also be for PC.

Best wishes

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Tue Jul 23 05:02:50 2002



From: Torsten.Fregin-at-zoologie.uni-hamburg.de
Date: Tue, 23 Jul 2002 11:56:53 +0200
Subject: Re: Software hunt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Steve,

why not use Powerpoint? OK, it is from Microsoft, but as most universities have a campus
licence for MS Office, and most students have it on their personal computers, too, it might be
a solution. And the students have to learn to work with all Office-programs anyway...

:-) Torsten




Torsten Fregin

Universität Hamburg - Zoologisches Institut
Abt. Neurophysiologie
AG Wiese - Raum 413
Martin-Luther-King-Platz 3
20146 Hamburg, Germany
Telefon *49-(0)40-42838-3931
Fax *49-(0)40-42838-3937
eMail Torsten.Fregin-at-zoologie.uni-hamburg.de
Torsten-at-Fregin.de





From daemon Tue Jul 23 07:02:07 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Tue, 23 Jul 2002 07:53:46 -0400
Subject: Re: Software hunt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

Another *REALLY* inexpensive solution that faculty and students here
have been using for posters has been Power Point (Really inexpensive if you
have MS Office already). Now power point has very little image manipulation
capability, so we fiddle images (EM, LM, Photo's, Graphs, Gels, etc.) else
where (Corel Photopaint, Adobe Photoshop, etc.), save as JPEG's or TIFF's
etc. and insert in Power Point. But Power point is easy to arrange images
and text.



} This is not a technical problem. I am seaching for graphics
} software for creating\printing posters and banners. We have poster
} sessions several times a year for the students. We were using an old
} DOS program but our computer lab has been upgraded(OS + network printers)
} and I can't get the program to work with the network printers.
}
} Does anyone have any suggestions for this software application? It has to
} be user friendly because of the students. And it has to be cheap because
} of the budget.
}
}
} Thanks,
}
} Steve Widing
} EM Tech / Computer Labs Manager
} Biology Department
} Temple University
} Philadelphia, PA



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Tue Jul 23 07:12:37 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Tue, 23 Jul 2002 14:04:59 +0200
Subject: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
What do you consider to be the standard terminology for microscopy involving
a conventional microscope with the sample illuminated by light?

Optical microscopy?

Light Microscopy?

Light optical microscopy?

Something else?

The first seems to be often used, but as far as I can see, every form of
microscopy is optical, whether using light, electrons, X-rays or something
else. So, it seems to be a bit too vague.

What should I use then?

Best wishes

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Tue Jul 23 08:42:51 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 23 Jul 2002 08:34:44 -0500
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
"Monson, Frederick C." {fmonson-at-wcupa.edu}
CC: "'List-Microscopy'" {Microscopy-at-sparc5.microscopy.com}
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From: Debby Sherman :      dsherman-at-purdue.edu
Date: 23 Jul 2002 08:34:44 -0500
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Fred,
I think your example is in extremely poor taste. What is legal and ethical involving one's own body is between that individual and their doctor, dentist, etc. and none of your business. This has little or nothing to do with scientific data.
Debby Sherman

On Monday, July 22, 2002 3:44 PM, Monson, Frederick C. {fmonson-at-wcupa.edu} wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jul 23 08:42:51 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 23 Jul 2002 08:36:42 -0500
Subject: Re: Software hunt

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From: Debby Sherman :      dsherman-at-purdue.edu
Date: 23 Jul 2002 08:36:42 -0500
Subject: Re: Software hunt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Steve,

Microsoft Powerpoint works fairly well if the posters are not huge. You just have to give the program enough memory. Go to Page Setup and set it for banner or custom (upper left) with your poster size inserted. This program is not as good as some drawing programs but is readily available on most computers and your printers should be able to handle it. Just remember that sometimes printers have a hardtime printing the background color. If that happens just make a box the size of the background and fill it with the color or pattern of preference. It should print fine.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907

On Monday, July 22, 2002 10:03 AM, swiding-at-temple.edu wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jul 23 08:54:41 2002



From: Christopher S. Zurenko :      czurenko-at-khri3.khri.med.umich.edu
Date: Tue, 23 Jul 2002 09:47:59 -0400
Subject: Re: Terminology: optical microscopy?

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I certainly do not have as much experience as many on this list
serve but with my training and experience to date, I have never
heard the term "optical microscopy" used. Microscopy using an
incandescent or "normal" bulb is typically referred to as "light
microscopy" or "bright-field microscopy".

Someone please correct me if I'm in error.

Cheers!

------------------------------------------
Christopher S. Zurenko
Research Assistant
Kresge Hearing Research Institute
The University of Michigan Medical School
MSRB 3, Room 9303
1150 W. Medical Center Drive
Ann Arbor, MI 48109-0648
Lab Phone: 734-763-9680
czurenko-at-umich.edu
http://www.khri.med.umich.edu/research/raphael_lab/index.htm


From daemon Tue Jul 23 08:55:41 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 23 Jul 2002 09:53:45 -0700
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I will propose a rough definition to be shredded, confirmed and clarified
by others.

First of all, I'm going to call it "light microscopy" instead of "optical
microscopy".

Light microscopy involves radiation from the UV (approximately 320 nm)
through the infra red (approximately 1100 nm). This can be transmittance
or reflectance. Typically, we mean by use of compound optics.


____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/



From daemon Tue Jul 23 10:27:08 2002



From: Tamara Howard :      thoward-at-unm.edu
Date: Tue, 23 Jul 2002 09:19:01 -0600 (MDT)
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How about "ROM"? ("Regular Old Microscopy"?!)

Tamara

On Tue, 23 Jul 2002, Ian MacLaren wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} What do you consider to be the standard terminology for microscopy involving
} a conventional microscope with the sample illuminated by light?
}
} Optical microscopy?
}
} Light Microscopy?
}
} Light optical microscopy?
}
} Something else?
}
} The first seems to be often used, but as far as I can see, every form of
} microscopy is optical, whether using light, electrons, X-rays or something
} else. So, it seems to be a bit too vague.
}
} What should I use then?
}
} Best wishes
}
} Ian MacLaren
} N.B. New address
} TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
} Petersenstr. 23, 64287 Darmstadt, Germany
} Tel: +49 6151 162894
} ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/
}
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Tue Jul 23 10:32:11 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 23 Jul 2002 11:25:29 -0400
Subject: RE: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK-1. I was told, by friendly, and private, warnings, that my example would
get a response like this. Well, OK-2. I have spent the last 20 minutes
carefully crafting an appropriate response, but in the end I decided to
remain 'friendly' rather than speak to a parenthetic, and impertinent, side
issue. Thus, the result of those minutes resides in my 'Draft' folder.

Now I want it clearly understood, that if I had received THIS message in
private, that I would have apologized, sincerely, for the pain I had caused.
But it wasn't friendly either.

The issue, however, IS imaging and how processing/alteration relate to
ethical and legal issues. I chose an example, BECAUSE it should have
focused on the many effects imaging/images have on our lives.

Finally, the fact that I married a beautifully proportioned woman when I was
25 was, when all was said and done, 95% her choice and less than 5% mine,
because I did listen to the opinions of others while I basked in the light
of my good fortune. Also, my Mother, at the age of 67, had a breast
reduction (is that Politically correct?), which was more for her comfort
and less for her appearance. Both of these occurrences must be the reason
for my lack of understanding that our discussion was about imaging and not
body parts, and that much of our imaging has been and will continue to be of
body parts from animals, people and automobile engines.

So let's stick to the issue, and don't make me send the other message which
was about defending my right of subject (the FIRST amendment???) selection.
And, if you don't like my choice of example, pick a better one and let's
discuss the real issue.

Respectfully,

Fred Monson

P.S. I will not comment further on this parenthetic unless suitably
provoked!

} ----------
} From: Debby Sherman
} Reply To: Debby Sherman
} Sent: Tuesday, July 23, 2002 9:34 AM
} To: 'Gary Gaugler'; Monson, Frederick C.
} Cc: 'List-Microscopy'
} Subject: Re: Ethical and legal issues in imaging
}
} Fred,
} I think your example is in extremely poor taste. What is legal and
} ethical involving one's own body is between that individual and their
} doctor, dentist, etc. and none of your business. This has little or
} nothing to do with scientific data.
} Debby Sherman
}
} On Monday, July 22, 2002 3:44 PM, Monson, Frederick C. {fmonson-at-wcupa.edu}
} wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi Gary,
} }
} } In imaging, it would be unethical for a female model to hand deliver a
} photo
} } which showed an amplified chest prior to surgery, unless she stipulated
} that
} } she is hopeful that she will look as she appears in the photo after her
} } surgery is done. If, on the other hand, she sent such photos thru the
} mail
} } without an accompanying declaration, she might be arrested for fraudulent
} } use of the mail.
} }
} } It is both legal and ethical for the model to send such an amplified
} photo
} } to past and future clients and ask if she has surgery and alters her
} } appearance as in the photo whether she will be more or less
} } employable.
} }
} } She can be both legal and ethical with her boyfriends by sending them
} such a
} } photo and asking whether she will be more or less enjoyable after such an
} } alteration.
} }
} } It would be illegal for the model to represent herself with an
} } enhanced chest in a public advertisement without the declaration. She
} would
} } also be leaving herself legally liable is she, unethically, used such a
} } photo to acquire employment on the pretence that she had the chest in the
} } photograph unless she arrived at the job with a note from her physician
} } attesting to the fact that between the sending of the photograph, her
} } employment and her arrival, her chest had suffered a deflation.
} }
} } I cannot think of anything more along these lines now. I would have
} } to see the original and altered photographs before reaching any other
} } conclusions.
} }
} } They may be sent to the address below.
} }
} } If a male model were to act in the manner of the female described
} } above, let the employer and boyfriend beware, there is NO legal or
} ethical
} } issue that can save them.
} }
} } Regards,
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging
} } Schmucker II Science Center
} } West Chester University
} } South Church Street and Rosedale
} } West Chester, Pennsylvania, USA, 19383
} } Phone: 610-738-0437
} } FAX: 610-738-0437
} } fmonson-at-wcupa.edu
} } CASI URL: http://darwin.wcupa.edu/casi/
} } WCUPA URL: http://www.wcupa.edu/
} } Visitors URL: http://www.wcupa.edu/_visitors/
} }
} }
} }
} }
} }
} }
} } } ----------
} } } From: Gary Gaugler
} } } Sent: Sunday, July 21, 2002 8:53 PM
} } } To: MSA listserver
} } } Subject: Re: Ethical and legal issues in imaging
} } }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Will the transactions at the conference be available
} } } either electronically or via print? This would be most
} } } interesting.
} } }
} } } I'm curious about the distinction between ethics and
} } } legal issues. Image analysis technology can produce
} } } legal data in cases where it was not possible in the past.
} } } I don't see that this has anything to do with ethics. It
} } } seems to me to be an issue of what can technology do
} } } and then what will the legal system accept? There are numerous
} } } instances of this issue. So far as I know, technology won.
} } }
} } } gary g.
} } }
} } }
} } }
} }
}
}


From daemon Tue Jul 23 10:36:26 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 23 Jul 2002 09:39:18 -0600
Subject: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is what the Encyclopedia Britannica has to say about "Optics":

science concerned with the genesis and propagation of light, the changes
that it undergoes and produces, and other phenomena closely associated with
it. There are two major branches of optics, physical and geometrical.
Physical optics deals primarily with the nature and properties of light
itself. Geometrical optics has to do with the principles that govern the...

(They would not give me more). Then there is this for "electron optics":

branch of physics that is concerned with beams of electrons, their
deflection and focusing by electric and magnetic fields, their interference
when crossing each other, and their diffraction or bending when passing very
near matter or through the spacings in its submicroscopic structure.
Electron optics is based on the wave properties of electrons, which,
according...

The Encyclopedia Britannica, however, uses "Light microscopy", not "optical
microscopy".

Both phrases are being used, and generally people understand what you are
saying regardless of what you are using. In fact, even "Light microscopy"
should be qualified further as in "Visible Light microscopy" (as opposed to
"Infrared microscopy").

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Tuesday, July 23, 2002 6:05 AM
To: Microscopy-at-sparc5.microscopy.com


Dear all,
What do you consider to be the standard terminology for microscopy involving
a conventional microscope with the sample illuminated by light?

Optical microscopy?

Light Microscopy?

Light optical microscopy?

Something else?

The first seems to be often used, but as far as I can see, every form of
microscopy is optical, whether using light, electrons, X-rays or something
else. So, it seems to be a bit too vague.

What should I use then?

Best wishes

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Tue Jul 23 11:09:05 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 23 Jul 2002 09:06:57 -0700
Subject: RE: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good points. But this is from the standpoint of the model--who
I presume did not prepare the manipulated photos. How do
ethics play into the "picture" relative to those who create them?

I can think of one. Suppose a person is accused of a crime.
Some optical evidence is manipulated to either acquit them or
condemn them. This would be un-ethical for the preparer either
way. But knowing that an image could be manipulated, would
the prosecutor or defense argue that the image was improperly
manipulated?

What I'm thinking of is the ability to see things that otherwise could
not be visualized. These things can make or break a legal event.

Check out:

http://www.imagecontent.com/lucis/whitepaper.html

especially the forensic application link.

gary g.

Disclaimer: I am an authorized dealer for Image Content products.
Lucis is used in forensic science.



At 01:44 PM 7/22/2002, you wrote:
} Hi Gary,
}
} In imaging, it would be unethical for a female model to hand deliver a photo
} which showed an amplified chest prior to surgery, unless she stipulated that
} she is hopeful that she will look as she appears in the photo after her
} surgery is done. If, on the other hand, she sent such photos thru the mail
} without an accompanying declaration, she might be arrested for fraudulent
} use of the mail.
}
} It is both legal and ethical for the model to send such an amplified photo
} to past and future clients and ask if she has surgery and alters her
} appearance as in the photo whether she will be more or less employable.
}
} She can be both legal and ethical with her boyfriends by sending them such a
} photo and asking whether she will be more or less enjoyable after such an
} alteration.
}
} It would be illegal for the model to represent herself with an
} enhanced chest in a public advertisement without the declaration. She would
} also be leaving herself legally liable is she, unethically, used such a
} photo to acquire employment on the pretence that she had the chest in the
} photograph unless she arrived at the job with a note from her physician
} attesting to the fact that between the sending of the photograph, her
} employment and her arrival, her chest had suffered a deflation.
}
} I cannot think of anything more along these lines now. I would have
} to see the original and altered photographs before reaching any other
} conclusions.
}
} They may be sent to the address below.
}
} If a male model were to act in the manner of the female described
} above, let the employer and boyfriend beware, there is NO legal or ethical
} issue that can save them.
}
} Regards,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} West Chester University
} South Church Street and Rosedale
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
}
}
}
}
} } ----------
} } From: Gary Gaugler
} } Sent: Sunday, July 21, 2002 8:53 PM
} } To: MSA listserver
} } Subject: Re: Ethical and legal issues in imaging
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Will the transactions at the conference be available
} } either electronically or via print? This would be most
} } interesting.
} }
} } I'm curious about the distinction between ethics and
} } legal issues. Image analysis technology can produce
} } legal data in cases where it was not possible in the past.
} } I don't see that this has anything to do with ethics. It
} } seems to me to be an issue of what can technology do
} } and then what will the legal system accept? There are numerous
} } instances of this issue. So far as I know, technology won.
} }
} } gary g.
} }
} }
} }



From daemon Tue Jul 23 11:18:10 2002



From: efosten-at-mmm.com
Date: Tue, 23 Jul 2002 11:12:28 -0500
Subject: RE: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Occam's Razor:~~ 'One should not increase, beyond what is necessary, the
number of entities required to explain anything'.

The late Walter McCrone used the term "Light Microscopy" (as do, today, the
institutions he founded) - who would argue with Walter.


Ev Osten

efosten-at-mmm.com
651-736-0104
fax: 651-733-0648

3M Company
Corporate Analytical Technology Center
3M Center, 201-BE-16
St. Paul, MN 55144-1000
U.S.A.



From daemon Tue Jul 23 11:59:25 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Tue, 23 Jul 2002 12:49:59 +0200
Subject: EM 201 "pepper" problem solved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I found out what was giving me this bad contamination of my grids in the Philps
EM 201. It was the O-ring which is located within the specimen holder. This
O-ring is sitting at the rod (which you move by pressing the button at the end
of the specimen holder) which pushes this "claw-mechanism" forward, thus
releasing the tip of the specimen holder with the grid. This O-ring and the area
around it was smeared with some slimy, black stuff. Maybe material of the O-ring
itself, but for sure way too much of some grease. So every grid I inserted got
his own cloud of dirt. Nice! Why it started so suddenly to give me this
contamination problem, I have no idea. But I know, sometimes things just start
all of a sudden to go wrong.
I should have thought much earlier about the inside of the specimen holder.
There are moving parts, telling me that there should be also an O-ring and
stuff. It is funny, I think because I worked for so long exclusively with a CM
10 and its "one-piece" specimen holder, I just did not see the problem. Scary,
how limited thinking can become if you get used to a specific system.

So Fred Monson was right with his statement:
So, here's what I think from what you have told me. All of a sudden, you are
getting the rain of death from the 201, and you have thought of everything but,
perhaps?????, an outgassing (old o-ring) phenomenon
associated with inserting the stage. The proverbial "cloud of dust".

It was such a good feeling to clean the specimen holder, put a grid in the scope
and everything is back to normal!


Thanks a lot for your help,

Stefan



From daemon Tue Jul 23 12:39:42 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Tue, 23 Jul 2002 13:32:17 -0400
Subject: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian,
I think it would be great if the term light microscopy were used.
TEM is definitely an optical microscopy technique.
You could argue about SEM. While the column is electron optical, the image
formation mechanism is not.
SPM is not with possible exception of near field.

My two cents. Or maybe one today.
The longer I work the farther I am from retirement!!!!
Russ Gillmeister
Xerox
~~~~~~~~~~~~~


-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Tuesday, July 23, 2002 8:05 AM
To: Microscopy-at-sparc5.microscopy.com


Dear all,
What do you consider to be the standard terminology for microscopy involving
a conventional microscope with the sample illuminated by light?

Optical microscopy?

Light Microscopy?

Light optical microscopy?

Something else?

The first seems to be often used, but as far as I can see, every form of
microscopy is optical, whether using light, electrons, X-rays or something
else. So, it seems to be a bit too vague.

What should I use then?

Best wishes

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Tue Jul 23 13:07:07 2002



From: dale anderson :      dale.anderson-at-attglobal.net
Date: Tue, 23 Jul 2002 13:58:27 -0400
Subject: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I asked this same question of Barry Carter and his response was the best
I've heard:

Visible light microscopy or VLM.

Ron

-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Tuesday, July 23, 2002 8:05 AM
To: Microscopy-at-sparc5.microscopy.com


Dear all,
What do you consider to be the standard terminology for microscopy
involving
a conventional microscope with the sample illuminated by light?

Optical microscopy?

Light Microscopy?

Light optical microscopy?

Something else?

The first seems to be often used, but as far as I can see, every form of
microscopy is optical, whether using light, electrons, X-rays or
something
else. So, it seems to be a bit too vague.

What should I use then?

Best wishes

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/




From daemon Tue Jul 23 13:21:11 2002



From: Gary Gill :      garygill-at-dcla.com
Date: Tue, 23 Jul 2002 13:13:39 -0500
Subject: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'll locate my reprint at home that will provide an answer and let you know
tomorrow. The great John R. Baker of England authored a series of 7 reports
of the Nomenclature Committee that were published primarily in the
Proceedings of the Royal Microscopical Society from 1967 thru 1970. One of
these provided the definition you seek.

Gary Gill


-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Tuesday, July 23, 2002 7:05 AM
To: Microscopy-at-sparc5.microscopy.com


Dear all,
What do you consider to be the standard terminology for microscopy involving
a conventional microscope with the sample illuminated by light?

Optical microscopy?

Light Microscopy?

Light optical microscopy?

Something else?

The first seems to be often used, but as far as I can see, every form of
microscopy is optical, whether using light, electrons, X-rays or something
else. So, it seems to be a bit too vague.

What should I use then?

Best wishes

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Tue Jul 23 14:41:33 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 23 Jul 2002 09:33:10 -1000 (HST)
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As much as the example of the model annoyed me, I am going to continue
with it to raise some points.

Let's say the model had her picture taken by one of her boyfriends, who
happened to have a camera with a long telephoto lens. Subsequently the
model purchases and uses the new Super-Duper-Triple-D Breast Enhancement
device, and diligently follows the instructions for use for three
weeks. She then has another picture taken.

Scenario 1: The second photo is taken by a different boyfriend, who has
his favorite super wide-angle lens on his camera, the one with the
pincushion effect that makes objects in the middle of the field appear
larger than those to the sides. He has no idea how the first photo was
taken.

Scenario 2: The second photo is taken by the first boyfriend, who
deliberately uses a wide-angle lens this time instead of the telephoto.

Scenario 3: In the first two scenarios the model genuinely thinks the
device has enlarged her bust.

Scenario 4: In the first two scenarios the model knows the device has NOT
increased her bust, but knows a bit about photography. She knows that the
telephoto lens will make her bust appear smaller due to foreshortening,
and the wide angle lens will make it look bigger, and has asked that those
particular lenses be used for the photos. The boyfriends are in on this or
they are not in on this.

Scenario 5: The photo shoots were not set up by an advertising agency for
the Super-Duper-Triple-D Breast Enhancement Device.

Scenario 6: The photo shoots were set up by an advertising agency for the
Super-Duper-Triple-D Breast Enhancement Device.

If you look at all the possible combinations and factor in whether each
participant knowingly or unknowingly performed their roles, and whether
they performed their part either knowingly and maliciously, or
unknowingly, or because they were uneducated about the properties of their
imaging devices, you can see that the images might or might not represent
the "truth". And this is WITHOUT digital or even darkroom
manipulation! Throw in differences in lighting and possibly the film type
and even the chemicals used to make the prints, and you see that you do
not have a controlled experiment here. Add a little personal bias (I might
personally consider the model to be an airhead and the first boyfriend to
be a fool and the second to be a power-hungry, manipulating character, and
so might draw conclusions based on personal bias when viewing the
images) and you've got not only flawed data, but a possible
misinterpretation of the data.

Now let's open these pictures in Photoshop ...

Aloha,
Tina


****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Tue Jul 23 15:24:38 2002



From: DrJohnRuss-at-aol.com
Date: Tue, 23 Jul 2002 16:16:11 EDT
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It's easy to imagine lots of scenarios in which the ability of the image to
fairly represent the truth might be compromised. But it is a lot more
interesting to consider some real cases. And still ones that do not require
actual "manipulation" of the image as those of us who do image processing and
use Photoshop think of it.

During the OJ mess, several newpapers and magazines printed pictures that
showed his face much darker and more shadowed than a "fair" image taken in
good light would have looked. It certainly made him appear more sinister. Was
that conscious bias or not? There have been arguments both ways. Along the
same lines, how about Richard Nixon's famous use of makeup to lighten a dark
5 o'clock shadow. That happened way before any picture was even taken. Was it
"fair" or not? Arguably, he did more damage to most americans than OJ.

News organizations are always being criticized for cropping of pictures. They
always claim it is just to fit things into the available space, but removing
other people and the surroundings from a picture can make it appear to be
something very different. They also don't always say that a picture of person
A with person B was taken 5 years ago and is file photo, and that those two
people were not together during some recent news event. This can create very
misleading impressions. It isn't just the tabloids that do it, either.

I think all of this is heavily overblown. For years I edited The Journal of
Computer Assisted Microscopy. Probably every issue had somewhere an image
captioned "typical" or "representative" microstructure. Nonsense - that was
widely understood to mean "the best picture we ever got" or worse yet "the
only good picture we ever got." No one picture can ever be representative in
a statistical sense. But if it was understood honestly by the author as
fairly representing that aspect of the structure that they were interpreting
or reporting on, then I say it is OK to use it.

Science is based on honest reporting and the inclusion of enough information
for others to duplicate our work. Sure, there will always be a few people who
try to cut a corner or shade the truth. I don't really care whether they do
it by selecting the field of view in the microscope, or by using the
Photoshop paint brush to remove some embarassing structure - it is wrong if
they knowingly introduce bias. And it is wrong if they don't know it but
should, but that gets a little bit trickier. Those who cheat are usually
caught in the end - science is a self-correcting process.

Worrying about whether applying an unsharp mask to an image to clean it up
for publication is misplaced concern. Most scientists know when they are
getting close to the line of fair representation, and choose not to cross it.
The nature of the tools is irrelevant. Telling people exactly what you did is
usually the best way to ensure that others can properly interpret your work.

In the broad sense, this applies to legal issues as well, but there the
problems get stickier. Experts may legitimately disagree about whether a
series of processing operations on an image reveal useful new information or
not, and it usually comes down to who is more convincing to the jury, at
least in the US/Canada/England legal system (please, let's not even think
about the French system!). I've been involved in these, been in the witness
box for days on end, and have written a book about it that says most of what
I have to say. This isn't the right forum for that debate anyway.

John Russ


From daemon Tue Jul 23 15:31:47 2002



From: Greg Strout :      gstrout-at-ou.edu
Date: Tue, 23 Jul 2002 15:25:48 -0500
Subject: EM Position

Contents Retrieved from Microscopy Listserver Archives
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EM POSITION IN MATERIALS RESEARCH
AT THE UNIVERSITY OF OKLAHOMA

Applicants are sought for a material-science research position at the
University of Oklahoma, in Norman, OK. The position is funded through
the NSF-funded OK-NanoNet, a Oklahoma- based network of researchers
involved in research involving nanostructures. The successful candidate
will participate in a team environment and must be familiar with
characterization of nanotubes and crystalline semiconductors including
the preparation of plan and cross-sectional samples. Experience with
HRTEM, diffraction techniques and image simulation is preferred. High
resolution SEM experience desirable.
Candidates should send a curriculum vita including a list of
publications, a statement of research interests and arrange for three
references letters to C-SPIN Program Manager, Department of Physics and
Astronomy, University of Oklahoma, Norman OK 73019-0225. Consideration
of applications will commence August 15th and continue until the
positions are filled. The University of Oklahoma is an Equal-Opportunity
Affirmative-Action employer.


--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Tue Jul 23 16:22:32 2002



From: Chaoying Ni :      cni-at-udel.edu
Date: Tue, 23 Jul 2002 17:14:23 -0400 (EDT)
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
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How about
visual light microscopy
laser microscopy
x-ray microscopy
electron microscopy
etc?



On Tue, 23 Jul 2002, Ian MacLaren wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} What do you consider to be the standard terminology for microscopy involving
} a conventional microscope with the sample illuminated by light?
}
} Optical microscopy?
}
} Light Microscopy?
}
} Light optical microscopy?
}
} Something else?
}
} The first seems to be often used, but as far as I can see, every form of
} microscopy is optical, whether using light, electrons, X-rays or something
} else. So, it seems to be a bit too vague.
}
} What should I use then?
}
} Best wishes
}
} Ian MacLaren
} N.B. New address
} TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
} Petersenstr. 23, 64287 Darmstadt, Germany
} Tel: +49 6151 162894
} ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/
}
}



From daemon Tue Jul 23 16:26:56 2002



From: Chaoying Ni :      cni-at-udel.edu
Date: Tue, 23 Jul 2002 17:20:33 -0400 (EDT)
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
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Still confusing. Per de Broglie, accelerated electron beam is light, so
does neutron......


On Tue, 23 Jul 2002, Michael Cammer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I will propose a rough definition to be shredded, confirmed and clarified
} by others.
}
} First of all, I'm going to call it "light microscopy" instead of "optical
} microscopy".
}
} Light microscopy involves radiation from the UV (approximately 320 nm)
} through the infra red (approximately 1100 nm). This can be transmittance
} or reflectance. Typically, we mean by use of compound optics.
}
}
} ____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
} (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
}
}



From daemon Tue Jul 23 16:41:47 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 23 Jul 2002 17:32:40 -0400
Subject: RE: Ask-A-Microscopist: paraffin wax

Contents Retrieved from Microscopy Listserver Archives
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Afternoon Dave,
If your specimen is already infiltrated with paraffin, then it is
likely that you are letting the paraffin on the surface of the infiltrated
specimen harden before you add the paraffin to fill the mold. If that is
true, then you need to do the following.
1. Find a piece of metal that is at least 1/8-1/4" thick
and around 7-8" square or in diameter
2. mount the metal about 7-10" above the table using one or
two clamps from chemistry
3. find a goose-neck lamp (60Watt bulb) and turn the hood
over so the light is directed toward the ceiling
4. slide the light under the metal plate leaving about 1"
between the hood and the plate
5. place an aluminum pie plate about the size of the metal
plate on the metal.
6. place a bit of paraffin in the pie plate and turn on the
lamp. If the paraffin is too hot, you should lower the lamp further from
the plate, or replace the 60W bulb with a 40W or a 25W. The temperature you
want is no higher than 60oCelcius.
7. when the paraffin begins to melt, place your cardboard
(folded from an index card to the right size for the specimen, but not less
than 1/2x1/2" in area) right on the melted paraffin and place a few pieces
of solid paraffin in the bottom of the box.
8. when THAT paraffin begins to melt, add the infiltrated
piece of specimen with the surface to be sectioned facing down.
9. when the specimen is covered with melted paraffin,
carefully remove the box to another pie plate on the table and watch while
the paraffin on the very bottom of the box begins to harden and trap the
specimen so it can't move. Prior to this you can use a warm dissecting
needle to position the specimen and tease trapped air bubbles to rise in the
paraffin.
10. you can move the box back-and-forth to the hot plate
until you are satisfied that the specimen is surrounded by melted paraffin.
11. when you are satisfied, and the specimen is trapped in a
slightly solid layer of paraffin, add sufficient paraffin to fill the box to
half its height but not less than 1/2" OR to 1/4" over the height of the
specimen.
12. If you are satisfied that the paraffin around the
specimen and the new, melted paraffin are merging nicely, and that no
paraffin is leaking from the box, you can fill the box to it's top (no more
than 1") with more melted paraffin and begin to blow lightly on the surface
of the melted paraffin.
13. When a layer of translucent paraffin forms that
prevents you from seeing the specimen in any detail you may VERY GENTLY and
SLOWLY immerse the block/box in cool water. NOTE: when the water is about
to spill over into the box, the box should be tilted slightly to permit the
water to slowly cover the paraffin. When the box is fully immersed, place
the container under a stream of running cold water so the paraffin block
will harden uniformly.
14. I generally leave the water running on my blocks while
I continue to make more blocks until I am finished. Then I let the last
block remain immersed in the running water for another 15min. Then I place
all my blocks in the fridge, or an ice bath, for at least a hour before I
begin to section any of them.
15. Trim before sectioning, and don't forget to turn off
the lamp!

Regards and hope this helps,

Fred Monson

P.S. If I did not correctly understand your problem, please come
back at me about your question.

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/



----------
} From: drands-at-avuhsd.k12.ca.us
} Sent: Saturday, July 20, 2002 3:16 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: paraffin wax
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (drands-at-avuhsd.k12.ca.us) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July
} 19, 2002 at 22:18:04
} --------------------------------------------------------------------------
} -
}
} Email: drands-at-avuhsd.k12.ca.us
} Name: Dave Rands
}
} Organization: Lancaster High School/Special Education Department
}
} Education: 9-12th Grade High School
}
} Location: Lancaster, California, Los Angeles County
}
} Question: We are having a difficult time getting our paraffin wax to
} fill in around our speciman. We have tried several things and have
} asked the other science teachers with no success. Can you help?
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Tue Jul 23 17:39:02 2002



From: Robert Blystone :      rblyston-at-trinity.edu (by way of
Date: Tue, 23 Jul 2002 17:26:29 -0500
Subject: Microscopy nomenclature

Contents Retrieved from Microscopy Listserver Archives
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To the List

If there is electron microscopy, can there be photon microscopy?

Bob Blystone


--
Robert V. Blystone, Ph.D.
Professor of Biology
Trinity University
San Antonio, Texas 78212
(210) 999-7243 or FAX (210) 999-7229
rblyston-at-trinity.edu
http://www.trinity.edu/rblyston


From daemon Tue Jul 23 17:57:05 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 23 Jul 2002 18:49:36 -0400
Subject: RE: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just Opinion Ian, and I hope not volatile or the source of physical
conflict.

And Ev, I couldn't help it. Further, I was finished when yours, with which
I entirely agree, arrived.

Light - those wavelengths in the electromagnetic spectrum(EMS) to
which WE (our visual systems) are sensitive (not reactive, meaning
illuminated or slightly heated, NOT burned!)

Optics - the study of the behavior of light, but, more broadly, the
behavior of any part of the EMS as it interacts with materials,
electromagnetic fields or objects.

Optical microscope - any microscope constructed of optical elements
constructed of glass or other transparent materials which by their
construction affect the passage of light.

Light microscope
any microscope that uses the "Visible" portion of the EMS to
illuminate an object for viewing OR which transmits visible light from an
object source stimulated by 'illumination' from a non-visible part of the
EMS (i.e. UV 'light'!?!?!?!, which is also known as 'black' 'light', or even
"invisible light" - one of the ultimate oxymorons)
sub-species of light microscopes
bright field
dark field
phase
fluorescent
etc.

Electronic microscope
any microscope whose image is formed by a non-visual
detector that is electrically powered.

Electron Microscope - a microscope whose image derives from the use
of electrons as illumination

X-Ray microscope - a microscope whose image derives from the use of
X-Rays as illumination

Atomic Force Microscope - an electronic microscope whose image is
formed by magic!

By the above definitions which, I opine, are generally held, one can
use the term, "Confocal Laser Scanning Microscope", because the term does
not stipulate that the illumination is "optical" or visual. Thus we are
safe in using the term CLSM to describe a system which has both UV and
Visual EMS wavelengths of excitation, as well as the term CLS(L)M ("L" =
light!), because the illumination from which the 'image' originates is
visible and the elements by which the illumination is manipulated are
correctly described as "optical" elements. On the other hand, and just to
add confusion, the filters on the excitation side are considered optical (or
visible) filters OR UV filters, while on the emission side, we describe all
of the filters as barrier filters.

I understand that the Physicists are attempting to alter the
definitions of "light" and "dark", and that suggests that most of the fun is
yet to come.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/



} ----------
} From: Ian MacLaren
} Reply To: Ian MacLaren
} Sent: Tuesday, July 23, 2002 8:04 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Terminology: optical microscopy?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} What do you consider to be the standard terminology for microscopy
} involving
} a conventional microscope with the sample illuminated by light?
}
} Optical microscopy?
}
} Light Microscopy?
}
} Light optical microscopy?
}
} Something else?
}
} The first seems to be often used, but as far as I can see, every form of
} microscopy is optical, whether using light, electrons, X-rays or something
} else. So, it seems to be a bit too vague.
}
} What should I use then?
}
} Best wishes
}
} Ian MacLaren
} N.B. New address
} TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
} Petersenstr. 23, 64287 Darmstadt, Germany
} Tel: +49 6151 162894
} ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/
}
}
}


From daemon Tue Jul 23 18:18:34 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 23 Jul 2002 17:16:57 -0600
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Tina,

I don't think we even have to consider just images. Any DELIBERATE
misrepresentation of data is unethical (at least scientifically. Ethics is
based on what is "morally" right, and "Morals" are not the same globally).
In your example, nothing is really unethical until the time when something
is claimed to be the case against better knowledge.

If I measure the resistance of a wire and find a big drop when I lower the
temperature, I can claim to have found some form of superconductor. Nothing
unethical about it. If I don't tell that I had a piece of a known
superconductor hooked up in parallel, now that would be unethical.

See you in Quebec!!

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Tina Carvalho [mailto:tina-at-pbrc.hawaii.edu]
Sent: Tuesday, July 23, 2002 1:33 PM
To: Microscopy Listserver


As much as the example of the model annoyed me, I am going to continue
with it to raise some points.

Let's say the model had her picture taken by one of her boyfriends, who
happened to have a camera with a long telephoto lens. Subsequently the
model purchases and uses the new Super-Duper-Triple-D Breast Enhancement
device, and diligently follows the instructions for use for three
weeks. She then has another picture taken.

Scenario 1: The second photo is taken by a different boyfriend, who has
his favorite super wide-angle lens on his camera, the one with the
pincushion effect that makes objects in the middle of the field appear
larger than those to the sides. He has no idea how the first photo was
taken.

Scenario 2: The second photo is taken by the first boyfriend, who
deliberately uses a wide-angle lens this time instead of the telephoto.

Scenario 3: In the first two scenarios the model genuinely thinks the
device has enlarged her bust.

Scenario 4: In the first two scenarios the model knows the device has NOT
increased her bust, but knows a bit about photography. She knows that the
telephoto lens will make her bust appear smaller due to foreshortening,
and the wide angle lens will make it look bigger, and has asked that those
particular lenses be used for the photos. The boyfriends are in on this or
they are not in on this.

Scenario 5: The photo shoots were not set up by an advertising agency for
the Super-Duper-Triple-D Breast Enhancement Device.

Scenario 6: The photo shoots were set up by an advertising agency for the
Super-Duper-Triple-D Breast Enhancement Device.

If you look at all the possible combinations and factor in whether each
participant knowingly or unknowingly performed their roles, and whether
they performed their part either knowingly and maliciously, or
unknowingly, or because they were uneducated about the properties of their
imaging devices, you can see that the images might or might not represent
the "truth". And this is WITHOUT digital or even darkroom
manipulation! Throw in differences in lighting and possibly the film type
and even the chemicals used to make the prints, and you see that you do
not have a controlled experiment here. Add a little personal bias (I might
personally consider the model to be an airhead and the first boyfriend to
be a fool and the second to be a power-hungry, manipulating character, and
so might draw conclusions based on personal bias when viewing the
images) and you've got not only flawed data, but a possible
misinterpretation of the data.

Now let's open these pictures in Photoshop ...

Aloha,
Tina


****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *

* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*

****************************************************************************



From daemon Tue Jul 23 18:30:36 2002



From: Eric.Hines-at-csiro.au
Date: Wed, 24 Jul 2002 09:23:43 +1000
Subject: eyepiece graticule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Does anyone know of a supplier of eyepiece graticules with radial lines at
known angles (a protractor) so we can measure angles through the microscope.
Eric.


From daemon Tue Jul 23 22:28:19 2002



From: RCHIOVETTI-at-aol.com
Date: Tue, 23 Jul 2002 23:19:03 EDT
Subject: Re: eyepiece graticule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 07/23/2002 4:40:19 PM US Mountain Standard Time,
Eric.Hines-at-csiro.au-at-sparc5.microscopy.com writes:

{ { Dear all,
Does anyone know of a supplier of eyepiece graticules with radial lines at
known angles (a protractor) so we can measure angles through the microscope.
Eric.

} }

Eric,

I don't know about reticles with angles, but one of my favorite reticle
suppliers here in the US is Klarmann Rulings.

They have a fairly extensive catalog, and they also will make custom reticles
per your specifications. We've been very pleased with the work they've done.

You can get contact information from their website at:
{www.reticles.com}

and here's (hopefully) the direct link: {A HREF="http://www.reticles.com/"}
Click here: Reticles, stage micrometers, filter glass, pinholes, apertures,
comparator screens, optical fabrication, evaporate {/A}

Cheers,

Bob Chiovetti
GTI Microsystems


From daemon Tue Jul 23 23:03:33 2002



From: R Divakar :      divakar-at-igcar.ernet.in
Date: Wed, 24 Jul 2002 09:26:38 +0530
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We metallurgists here call it optical metallography meaning we use the visible region of the EM spectrum for imaging metallic surfaces.

----
Divakar R
Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----

-----Original Message-----
} From: Michael Cammer [SMTP:cammer-at-aecom.yu.edu]
Sent: Wednesday, July 24, 2002 9:21 AM
To: Ian MacLaren; Microscopy-at-sparc5.microscopy.com


I will propose a rough definition to be shredded, confirmed and clarified
by others.

First of all, I'm going to call it "light microscopy" instead of "optical
microscopy".

Light microscopy involves radiation from the UV (approximately 320 nm)
through the infra red (approximately 1100 nm). This can be transmittance
or reflectance. Typically, we mean by use of compound optics.


____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/






From daemon Tue Jul 23 23:29:13 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 23 Jul 2002 21:22:36 -0700
Subject: RE: Ethical and legal issues in imaging

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Very good point Mike and Tina: Any DELIBERATE misrepresentation of data is
unethical in science, I believe. Sergey

At 04:16 PM 7/23/02, you wrote:
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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Jul 23 23:47:52 2002



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Tue, 23 Jul 2002 23:44:59 -0700
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
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John Russ makes some important points. Integrity in presenting information isn't
a question only of manipulation of the data itself -- whether "high tech" or not
-- but can also involve much more subtle kinds of efforts to shade the perception
of the recipient. For example, in presenting data or opinions, one might
exaggerate or misrepresent one's qualifications -- though this does not actually
alter the validity of the data or opinions expressed, it does represent an
attempt to influence their credibility. In many fields of endeavor this is, of
course, the accepted norm, and even in the scientific arena, the line quickly
becomes quite fuzzy -- when does an effort to make a convincing case pass over
into deceit?

Ultimately, our best guarantee of integrity in scientific data is the integrity
of the one reporting. The key, it seems to me, is not simply whether the one
offering the data or making the assertion "honestly believes" in the conclusion,
but also exercises a high degree of personal scrupulousness as to whether the
recipient is given enough information to make their own objective assessment.
Often this is less about clear "rules" than about adhering to accepted norms of
behavior. For example, a researcher who consistently observes a particular
phenomenon is not considered to be deceitful if she/he chooses a particularly
clear-cut example for illustration -- this is what we expect. But, we don't
expect a scientist to support his/her conclusion by selecting an untypical
example -- this violates the trust of the community. Or to use the above
example, we expect that a researcher will represent their personal qualifications
in a generally favorable light -- but we don't expect to have to check the
accuracy of their resume. A scrupulous scientist endeavors to be scrupulous
about not only the bare facts, but also about the "frame" in which they are
presented.

In the ultimate analysis, there aren't hard-and-fast rules which always apply and
there will always be argument about where the line should be drawn. But this is
why it is so important that we police ourselves -- when we do see clear intent to
alter, misrepresent, withhold or otherwise bias information, we have an
obligation to "blow the whistle" -- even when such actions do not materially
alter the conclusion.

The above, of course, is about ethics. But I tend to be one of those who thinks
that if people behave ethically, the "legal" issues pretty much take care of
themselves.

Fred Schamber



"DrJohnRuss-at-aol.com"-at-sparc5.microscopy.com wrote:

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}
} It's easy to imagine lots of scenarios in which the ability of the image to
} fairly represent the truth might be compromised. But it is a lot more
} interesting to consider some real cases. And still ones that do not require
} actual "manipulation" of the image as those of us who do image processing and
} use Photoshop think of it.
}
} During the OJ mess, several newpapers and magazines printed pictures that
} showed his face much darker and more shadowed than a "fair" image taken in
} good light would have looked. It certainly made him appear more sinister. Was
} that conscious bias or not? There have been arguments both ways. Along the
} same lines, how about Richard Nixon's famous use of makeup to lighten a dark
} 5 o'clock shadow. That happened way before any picture was even taken. Was it
} "fair" or not? Arguably, he did more damage to most americans than OJ.
}
} News organizations are always being criticized for cropping of pictures. They
} always claim it is just to fit things into the available space, but removing
} other people and the surroundings from a picture can make it appear to be
} something very different. They also don't always say that a picture of person
} A with person B was taken 5 years ago and is file photo, and that those two
} people were not together during some recent news event. This can create very
} misleading impressions. It isn't just the tabloids that do it, either.
}
} I think all of this is heavily overblown. For years I edited The Journal of
} Computer Assisted Microscopy. Probably every issue had somewhere an image
} captioned "typical" or "representative" microstructure. Nonsense - that was
} widely understood to mean "the best picture we ever got" or worse yet "the
} only good picture we ever got." No one picture can ever be representative in
} a statistical sense. But if it was understood honestly by the author as
} fairly representing that aspect of the structure that they were interpreting
} or reporting on, then I say it is OK to use it.
}
} Science is based on honest reporting and the inclusion of enough information
} for others to duplicate our work. Sure, there will always be a few people who
} try to cut a corner or shade the truth. I don't really care whether they do
} it by selecting the field of view in the microscope, or by using the
} Photoshop paint brush to remove some embarassing structure - it is wrong if
} they knowingly introduce bias. And it is wrong if they don't know it but
} should, but that gets a little bit trickier. Those who cheat are usually
} caught in the end - science is a self-correcting process.
}
} Worrying about whether applying an unsharp mask to an image to clean it up
} for publication is misplaced concern. Most scientists know when they are
} getting close to the line of fair representation, and choose not to cross it.
} The nature of the tools is irrelevant. Telling people exactly what you did is
} usually the best way to ensure that others can properly interpret your work.
}
} In the broad sense, this applies to legal issues as well, but there the
} problems get stickier. Experts may legitimately disagree about whether a
} series of processing operations on an image reveal useful new information or
} not, and it usually comes down to who is more convincing to the jury, at
} least in the US/Canada/England legal system (please, let's not even think
} about the French system!). I've been involved in these, been in the witness
} box for days on end, and have written a book about it that says most of what
} I have to say. This isn't the right forum for that debate anyway.
}
} John Russ



From daemon Tue Jul 23 23:51:51 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 23 Jul 2002 21:44:27 -0700
Subject: RE: Terminology: optical microscopy?

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Not agree, Fred:
" Optical microscope - any microscope constructed of optical elements
constructed of glass or other transparent materials which by their
construction affect the passage of light."====} not necessary light, may be
electrons, or even gamma-rays (gamma-ray microscope) - everything which has
a 'waive nature' irradiation. In EM the optics are based on the
electro-magnetic lenses. I think, 'optics' it's more like technical
solution to manipulate the wave-nature irradiation. Combination of the
lenses and mirrors is usual solution for the microscopes. Material should
be adequate to the irradiation: glass for visible light, quartz for UV,
KCl(Li-?) - for infra-red light and so on... So, we could talk about
'electro-magnetic optics' of the particular EM or about infra-red optics...
We could say: optics for the visual light microscope or UV light
microscope... Sometimes it needs to be precise using terminology, which
comes from the physics - those guys are very precise. Sergey

At 03:49 PM 7/23/02, you wrote:
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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Jul 24 01:15:37 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Tue, 23 Jul 2002 23:05:10 -0400
Subject: Re: Terminology: optical microscopy?

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on 7/23/02 5:20 PM, Chaoying Ni at cni-at-udel.edu wrote:


}
} Still confusing. Per de Broglie, accelerated electron beam is light, so
} does neutron......
}
}
Dear Chaoying Ni,
Well, an accelerated electron emits light and is a wave, but it is not
light (to a real physicist, it gets heavier as its speed increases).
Focussing neutron beams is a challenge; using the magnetic moment to apply
force might be possible. Perhaps one ought to say "visible photon
microscopy", but that will not be the standard terminology.
Yours,
Bill Tivol



From daemon Wed Jul 24 01:32:59 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Tue, 23 Jul 2002 23:24:16 -0400
Subject: Re: Terminology: optical microscopy?

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on 7/23/02 6:49 PM, Monson, Frederick C. at fmonson-at-wcupa.edu wrote:

Dear Fred,
First, by processing your message, I hope I have not done anything
unethical.
}
} Just Opinion Ian, and I hope not volatile or the source of physical
} conflict.
}
Likewise, unless by "physical conflict" you mean that physicists
disagree.

} (i.e. UV 'light'!?!?!?!, which is also known as 'black' 'light', or even
} "invisible light" - one of the ultimate oxymorons)
}
You know I'm keeping this part in for a reason.

} Electronic microscope
} any microscope whose image is formed by a non-visual
} detector that is electrically powered.
}
}
} Atomic Force Microscope - an electronic microscope whose image is
} formed by magic!

As, "When technology becomes sufficiently advanced, it is equivalent to
magic." (Can't remember the author or exact quote.)
}
} By the above definitions which, I opine, are generally held, one can
} use the term, "Confocal Laser Scanning Microscope", because the term does
} not stipulate that the illumination is "optical" or visual. Thus we are
} safe in using the term CLSM to describe a system which has both UV and
} Visual EMS wavelengths of excitation, as well as the term CLS(L)M ("L" =
} light!),

However since laser is the acronym for "Light Amplification by
Stimulated Emission of Radiation", then the "light" in CLSM is, indeed,
invisible, and CLS(L)M is redundant. Maybe C(V)LSM?
}
} I understand that the Physicists are attempting to alter the
} definitions of "light" and "dark", and that suggests that most of the fun is
} yet to come.
}
I think "light" and "dark" remain the same, but most of the matter and
energy will prove to be dark. Now, could we investigate them by dark-field
microscopy? I agree; the fun's just beginning.
Yours,
Bill Tivol



From daemon Wed Jul 24 01:40:46 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 23 Jul 2002 23:35:16 -0700
Subject: Re: Microscopy nomenclature

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Bob:

Google search "photon microscopy" - 26000 articles
http://www.google.com/search?hl=en&ie=UTF-8&oe=UTF-8&q=photon+microscopy
Sergey

At 03:26 PM 7/23/02, you wrote:
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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Jul 24 03:23:02 2002



From: ankohangela :      ankohangela2002-at-yahoo.co.uk
Date: lun., 15 juil 2002 09:46:12
Subject: please make conscience to my plea

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Hi there,

probably i did not get the whole thread, but did anybody mention
OpenOffice ? (see http://www.openoffice.org) - It is OpenSource, so the
only thing you have to take care about is how you get hold of the
software package for computer-system: For Linux, Solaris and Win32-
Systems there should be version 1.01 available by now. A version 1.0x
for MacOS X will hopefully be available by the end of this year.

No fee's, no trick's, no backdoor's, no legal annoyancies ... !!!

Up to now we made pretty good experiences when composing our
posters. (Ok, every program has bugs, but we found appropriate help in
the OpenOfffice user/developer community)

If you would like to have a professional spellchecker and thesaurus, you
will have to spend a few $ and try StarOffice 6.0 (... same source code
like OpenOffice!!!)

.. and the best thing is: the import/export filters to dif. MS-Office appl.
are pretty good so you will be able to communicate with BiilyG's
"people")


..try it!
Gunnar








Date sent: 23 Jul 2002 08:36:42 -0500
} From: Debby Sherman {dsherman-at-purdue.edu}

This is a multi-part message in MIME format.

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boundary="----=_NextPart_ZAKWWATYQD"

------=_NextPart_ZAKWWATYQD
Content-Type: text/plain;charset="iso-8859-1"
Content-Transfer-Encoding: 7bit

Dear sir,



PLEASE REPLY VIA THIS EMAIL FOR EASY ACCESSING.
(ankohangela2002-at-yahoo.co.uk)

Please permit me to use this medium to thank you once more
for given me this avenue to explain my problem to you.

But firstly, i will like to tell you who am so that you
will understand and comprehend my point clearly.

Am angela ankoh and am here in Benin Republic with my
junior brother David and we are from Congo Democractic but
now in Benin Republic as a result of the war in my
country. And here in benin we entered as a refugee as a
result of the war in my country. And am still a studet and
my brother too. Am 24 years and david is 21. am studying
medicine and my brother also is mechanical engineering

Please we need your help because the war has claimed the
life of both my father and mother leaving us alone in this
world. But before the death of my father, he used my name
to deposit some money WHICH VALUED AT 9 MILLION UNITED STATE DOLLARS in
a finance house here in republic of benin and and he used
me as the beneficiary and owner of the money but since we
entered here as a refugee i cannot have access to my money
because i do not have account here and they do not allow
refugees to operate account here.

Please what i need from you is to help us settle the finance house for their clearing fee and open an account
with your name in any bank here in benin so that i can
submit your particulars to the finance house to effect the
change of the ownership in your name as the new
beneficiary and after they will change the name to your
favour and they will deposit in your bank here. And you
will then instruct the bank here to further transfer to
you account over there so that we can come over there for
the continuation of our education while you help us to
manage the money;

furthermore, what i actually need from you is to help us
clear the money from the finance house to aviod any
dumorage on the moneyand after making the change of the benficiary to your name the finance house will deposit in your account here and you then can further transfer to your account over for our onward coming for the continuation of our education while you help us to control the money because the finance house and my
late father had an agreement when the money will be in
their custody and if we fail to come and claim as agreed
that will be charging for it. and i do not want it to
heppen because this is the only hope of our life.

secondly, you will be required to open an account here in
benin republic where the finance house will deposit and
upon your instruction to the bank after the finance hoiuse
deposited the m bank will further transfer according to
your wish to any of your account you prefer,and we proceed
with you for the continuation of our education.in this
regard you are only required to clear and open an account
and the finance house deposit in your account here..

please if you are ready to help us, please kind forward the
following so that i can effect the change of the
beneficiary to your name as the new beneficiary so that
once you arrive and do the clearing and open the account
they will deposit it in your account immediately and you
have access to the money.

I NEED YOUR FULL NAME, ADDRESS AND TELEPHONE NUMBER .THIS
WILL ENABLE ME TO PROCEED FOR THE CHANGE OF THE OWNERHSIP
IN YOIUR FAVOUR AS THE NEW BENEFICARY OF THE MONEY.

GOD BE WITH US ALL AS WE AWAIT YOUR POSITIVE ANSWER.

FROM ANGELA ANKOH.

NOTE YOU CAN AS WELL REACH ME OR DAVID ON THIS NUMBER THE COUNTRY CODE IS 229 AND THE NUMBER IS 989857. I REPEAT 229-989857.AND EMAIL ankohangela2002-at-yahoo.co.uk



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From daemon Wed Jul 24 04:23:56 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 24 Jul 2002 10:16:44 +0100 (BST)
Subject: Re: liquid He transferring kids

Contents Retrieved from Microscopy Listserver Archives
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Hi Jinsong,

You do not say if it is a SEM or TEM He holder. My comments relate to our
experience with our TEM He holder but I guess most would also apply to a
SEM He stage.

We have a side entry He holder that uses the He boil off gas to precool
the He dewar so that we do not have a Liquid N2 jacket.

We do not loose vacuum when filling but we do support the transfer tube to
prevent too much strain on the holder.

The initial fill takes about 45 minutes. It is best for 2 people to set up
and remove the He transfer tube, although it is possible for a single
person to do it it just seems safer with two.

Depending on temperature the hold time is generally about 50 mins to 3
hours (50 mins at 7K or 8K, 2 hours at 15K, 2.5 hours at 21K etc.).

Subsequent transfers take about 30 minutes.

As we tend to use the holder infrequently and book a week of He stage work
when we want it we will always repump the vacuum jackets on the transfer
dewar and holder before each session.

The most diffucult thing is to determine when the dewar is full and on the
first trial we shot most of a 50l dewar of He through the holder as we
could not tell the difference between the He gas plume and the He liquid
plume on the exhaust line. With experience we can now fill with about 5L
of He.

It is important to use the gas to cool the holder down to a reasonable
level (50K) to avoid loosing too much liquid.

I would suggest that if you have problems you contact the supplier to get
advice on filling technique.

Good luck,
Ron


On Mon, 22 Jul 2002, jinsong wu wrote:

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} -----------------------------------------------------------------------.
}
}
} Hi, Listers:
}
} We have a new liquid-He holder. However, it takes three men to
} work together for about one and half hours for every filling up of the
} holder. At the same time, there is high risk to destroy the vacuum.
} Then we have merely about 30 minutes' working time.
}
} Please let me know your kind suggestions on the possible techniques
} of the transferring of the liquid He from the tank to the holder.
} For example, is it possible to put the He tank in near room and use
} some transferring kits connecting the tank and the holder so as to be
} able to work continuously?
}
} Any replies from commercial sources or vendors are welcome to my
} personal email address: jinsong.wu-at-asu.edu.
}
} Thanks a lot,
}
} jinsong wu
} Department of Physics and Astronomy
} Arizona State University
} Tempe, AZ 85287-1504
}
} Tel: 480-965-2535 (o)
}
}
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================





From daemon Wed Jul 24 04:40:49 2002



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Wed, 24 Jul 2002 11:33:25 +0200
Subject: Fwd: Re: Terminology: optical microscopy?

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With all due respect for the participants to the discussion, I'd like to draw
your attention on the oversimplifications you are introducing here.

Generally, the "accelerated" electrons can emit X-rays (that is the basic
principle of synchrotron X-ray generation), which are electromagnetic waves,
as light is too.

It is not correct to say that the (accelerated or not) electron or the
neutron are light;
they have an associated wavelength (according to quantum mechanics), but they
are not waves. In fact, the quantum mechanical wavelength associated
(according
to the de Broglie principle) to any particle (but "detectable" only for the
case of
elementary particles) is a matter that generates a lot of confusion.

The people who are not paying enough attention to these notions can be led to
express even stranger opinions, as was the case of an attendant to a
conference
on electron microscopy, who was strongly against the opinion that the
photon has
mass zero; how can that happen - according to him - as long as the
electron has
an impulse ? Well, it happens "just like that" !

So, please, be careful when discussing that kind of matters: quantum
mechanics is
a little more profound than to say "electron is light, and so is neutron"
etc. These are
nothing but confusions.

I have expressed that opinion as a physicist.


Corneliu Sarbu, PhD
Department of Metallurgy and Applied Materials Science (MTM Dept.)
Catholic University of Leuven (KULeuven)
Kasteelpark ARENBERG nr. 44
B-3001 Heverlee-Leuven, Belgium
****************************************************************
Phone: +32-16-32.1241 - office
+32-16-32.1264 - secretary of department
Fax: +32-16-32.1992 or +32-16-32.1270
e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be
****************************************************************


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} Date: Tue, 23 Jul 2002 23:05:10 -0400
} Subject: Re: Terminology: optical microscopy?
} From: Bill & Sue Tivol {wtivol-at-earthlink.net}
} To: microscopy list {microscopy-at-sparc5.microscopy.com}
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From daemon Wed Jul 24 04:57:07 2002



From: Allen Sampson :      ars-at-sem.com
Date: Wed, 24 Jul 2002 05:31:06 -0700
Subject: RIP (hopefully) Ethical and legal imaging and nomenclature of microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there,

probably i did not get the whole thread, but did anybody mention
OpenOffice? (see http://www.openoffice.org) - It is OpenSource, so the
only thing you have to take care about is how you get hold of the
software package for your computer-system: For Linux, Solaris and Win32-
Systems there should be version 1.01 available by now. A version 1.0x
for MacOS X will hopefully be available by the end of this year.

No fee's, no trick's, no backdoor's, no legal annoyancies ... !!!

Up to now we made pretty good experiences when composing our
posters. (Ok, every program has bugs, but we found appropriate help in
the OpenOfffice user/developer community)

If you would like to have a professional spellchecker and thesaurus, you
will have to spend a few $ and try StarOffice 6.0 (... same source code
like OpenOffice!!!)

.. and the best thing is: the import/export filters to dif. MS-Office appl.
are pretty good so you will be able to "communicate" with BiilyG's
"people".

..try it!
Gunnar



Date sent: 23 Jul 2002 08:36:42 -0500
} From: Debby Sherman {dsherman-at-purdue.edu}


Sorry, but the number of postings on these two subjects is driving me
crazy. So, hopefully, here's the start of a new round of messages related
to the ethics of imaging and the nomenclature of microscopy techniques.

First, in regard to ethics and legal imaging, one really has to question
the need to tie ethics to one of the most unethical professions in the
world. Be that as it may, ethical behavior is just that - behavior that
each individual chooses to follow. Civilization is just a consensus
between humans as to what is acceptable and desirable in all of us. It is
a noble cause which often results in great things such as the scientific
principles and the law. However, there are always those among us who are
willing to subvert those ideals to our own individual desires.

Some find a fine line between what they want to portray and what is by
measurement. All too easy these days to wash away what we might consider
unimportant. But true discovery often lies in those details that most
would overlook. That applies whether it is a legal or scientific matter.
We are all creatures of assumptions, and those assumptions are often
wrong. What is important in a legal matter is to portray what you believe
and let your credibility lend weight to the testimony. There will be
others doing the same for your side, and the other. The law is a search
for the truth of a matter, the same as science. The difference is one of
time. Science discovers the truth over years, decades, centuries and
millennium. The law has to proceed in a timely manner, governed by the
life spans of humans. Science can afford mistakes, and often makes them.
In law, a mistake can mean the immediate difference between life and
death.

Better to simply do your best, represent the data as you found it, and
leave the interpretation to those unfortunate souls who actually have to
make the life and death decisions. Judge, jury and executioner are
assigned by law, not science. Thank God, because science has the
unfortunate position of being corrected rather regularly. Human decisions,
too, have the unfortunate position of often being wrong. But the law is
designed for that, not science. Law is about humans, science is about
everything else. Until the time there is actually any reasonable total
understanding (if ever) of the biology, environment and intellect of
humans, science can have no real claim on matters of law.

In brief (way too late), don't assume that you know what will play best
before the audience. Even simple manipulations shade the truth. A trained
eye can recognize the subtle lighting effects used to portray the before
and after effects of tooth whitening and wrinkle removers common on TV ads.
But realize that millions are spent by those who don't. Should we
regulate the production of ads (do you want the government to spend your
money on that)?

OK, on to nomenclature. Lets start trying to come to a simple and pract
ical consensus on 'microscopy'. How about a means to produce an enlarged
image of something. Not enhanced, just enlarged. In a sense, a means to
produce a closer look.

A prefix to this would seem to be the major problem. 'Optical' is probably
outdated. Done in by the common use of optics to also describe the
electrostatic and electro-magnetic effects used in electron microscopy.
Better to use the term optics to describe the various refraction and
reflection mechanisms in general. In this context, all light, x-ray,
gamma-ray and electron manipulation systems could be termed 'optical'.

Light, x-ray and gamma-rays do have a definable basis. All involving
photons, there are definitions regarding their generation. The actual
wavelengths may overlap, but the mechanisms of their generation do provide
a recognizable distinction. They can all be considered optical, perhaps
classically so, because they use materials to provide the refraction and
reflection required (grazing incidence angle optics in the case of x-ray
and gamma-rays).

Electrons comprise a transition from classical optics to electrostatic and
electro-magnetic optics. They can be manipulated by materials (electron
diffraction) as well as electronic means.

All right, getting to specifics. All of the energies mentioned above can
be used in both transmission and reflective modes. This begins the general
classification. The first class should be 'transmission microscopy',
techniques that provide an image from passing the source energies though a
sample. This would include substage illumination light microscopy, TEMs
and normal x-ray imaging.

Next general classification should be 'reflective microscopy'. This would
encompass the techniques of reflective light microscopy, SEM and potential
x-ray and gamma-ray reflective microscopy (I don't know if these techniques
have been developed yet, but could be). These are basically contour
mapping techniques that essentially record surface slopes in regards to the
primary source of illumination.

A third general classification should be made for the more recent scanning
probe instruments. These are basically topographical methods that record
the topography of material surfaces based on various physical properties.
Their progenitors are the profilometer and Edison's record player. I
would suggest the term 'profile microscopy' for these techniques, as they
produce a profile of the topography at various one dimensional locations.

Within those general classifications, one should distinguish the energy
involved. For example, TEM could still remain transmission electron
microscopy, but SEM should perhaps be changed to Reflective Electron
Microscopy or REM. Seems silly here, but consider the case of STEM. The
actual imaging mode in STEM is determined by the placement of the detector
used. In the case of a secondary or BSE detector located above the sample,
it would be REM. Where the image is formed by detection means below the
sample it would be TEM. There are TEMs that produce STEM images that look
like SEM and SEMs that produce STEM images that look like TEM. STEM, by
itself, says nothing - better to make scanning a sub grouping. And that
brings up the actual item being detected - secondary electron, backscatter
electron, Auger electron or x-ray (anything I missed?). We won't go there
now.

So, light or optical microscopy would become light transmission or light
reflective microscopy. A mixture of the two, light transmission/reflective
microscopy. So it goes through electron, x-ray and gamma-ray (and anything
else we can come up with). Scanning probe microscopy (profile microscopy)
should be further identified by the detection means, as they currently are
(minus the 'profile').

Confused yet? I am. And we haven't even gotten to the confocal and
holographic methods yet. Suffice to say, either live with the current
nomenclature or find some common taxonomy for imaging techniques. Frankly,
we're getting to the point where it would be nice to have a taxonomy for
these techniques.

What's in a name?



Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com





From daemon Wed Jul 24 06:13:41 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Wed, 24 Jul 2002 07:06:56 -0400
Subject: Re: eyepiece graticule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had a special eye piece that I used for accurately measuring diatom
striations that would read out to some small fraction of a degree - they
are called filar ocular micrometers and are still available for some
microscopes.


Bill Miller


At 11:19 PM 7/23/2002 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jul 24 07:23:58 2002



From: swiding :      swiding-at-temple.edu
Date: Wed, 24 Jul 2002 08:15:52 -0400
Subject: Software hunt update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List,

Thanks for all the suggestions. What I am looking for is a graphics
program that will print banners on sheet paper (8.5 x 11). Our old program
would spread the banner across the sheets. After printing, you would put
the sheets together for the banner. We have Canvas, Photoshop, Paint Shop Pro,
Powerpoint, etc. software but as far as I can determine none of them have
this feature.

Thanks,

Steve Widing
EM Tech / Computer Labs Manager
Biology Department
Temple University
Philadelphia, PA



From daemon Wed Jul 24 08:41:36 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 24 Jul 2002 09:33:25 -0400
Subject: RE: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lets pose a question:

What is microscopy? The use of some form of the EMS to examine an
object's properties.

What are Optics? Are they not the component of the system that allows
us to harness and control the particular range of the EMS we want to
use?

I don't agree that you can separate Optics as being only for the use of
longer wavelength (Visual spectrum or say UV-IR).

Optics should be as defined by physics. Any lens element that changes
the character or distribution of any component of the EMS.

I'm going through a small library behind me here are a few snippets:

"The transmission electron microscope is a type of microscope and thus
conforms to the definition of a microscope which is "an optical
instrument consisting of a lens or a combination of lenses used for
making enlarged or magnified images of minute objects.""
-Clinton J. Dawes 'Biological Techniques for Transmission and Scanning
Electron Microscopy 1979 edition published by Ladd. - third printing

That's just one good example.

Optical Microscopy: does that mean it uses a lens system to examine an
object? If so then it becomes impossible to separate the Electron
Microscopes from Light microscopes.

Here are a few terms I use:
CLM: Conventional Light Microscopy (I learned from Dr. Joanne Whallon
and Dr Shirely Owens at Michigan Sate University)
This has been shortened to just:
LM: Light microscopy with the assumption made that the light is between
UV and IR

And yes - I will get directly to the posed question so as to say on
topic and focused to Ian MacLaren's original Email:

Light Microscopy works very well and I think deserves the position of
being the 'correct' term. LM is a nice simple abbreviation that goes
very well with TEM, SEM, AFM, STEM, LSM, CLSM, LSCM (those confocal
folks need to settle down and pick LSM ;) ).

Make any sense?

Geoff Williams
Microscopy Facility Supervisor
Biology Department
Central Michigan University
Mt Pleasant, MI 48859



From daemon Wed Jul 24 08:41:36 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Wed, 24 Jul 2002 15:33:38 +0200
Subject: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Any suggestions for the best electropolishing solution and conditions for a
Nickel-based superalloy (CMSX-4). We have a Struers Tenupol.

Thanks

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Wed Jul 24 08:50:45 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 24 Jul 2002 08:44:44 -0500
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All of these comments raise the issue that there should be a more
rigorous photography component to any course that teaches microscopy,
whether as a field of study or as a tool.
All good microscopy courses teach photography to some extent, but
this should be expanded, and include more theory. And more material
normally considered part of the photography courses taught in art
schools.
This would have obvious influences on training on the legal and
ethical issues of imaging in microscopy. Perhaps education should be
a part of the ethics sessions at M&M.
Phil

} As much as the example of the model annoyed me, I am going to continue
} with it to raise some points.
}
} Let's say the model had her picture taken by one of her boyfriends, who
} happened to have a camera with a long telephoto lens. Subsequently the
} model purchases and uses the new Super-Duper-Triple-D Breast Enhancement
} device, and diligently follows the instructions for use for three
} weeks. She then has another picture taken.
}
} Scenario 1: The second photo is taken by a different boyfriend, who has
} his favorite super wide-angle lens on his camera, the one with the
} pincushion effect that makes objects in the middle of the field appear
} larger than those to the sides. He has no idea how the first photo was
} taken.
}
} Scenario 2: The second photo is taken by the first boyfriend, who
} deliberately uses a wide-angle lens this time instead of the telephoto.
}
} Scenario 3: In the first two scenarios the model genuinely thinks the
} device has enlarged her bust.
}
} Scenario 4: In the first two scenarios the model knows the device has NOT
} increased her bust, but knows a bit about photography. She knows that the
} telephoto lens will make her bust appear smaller due to foreshortening,
} and the wide angle lens will make it look bigger, and has asked that those
} particular lenses be used for the photos. The boyfriends are in on this or
} they are not in on this.
}
} Scenario 5: The photo shoots were not set up by an advertising agency for
} the Super-Duper-Triple-D Breast Enhancement Device.
}
} Scenario 6: The photo shoots were set up by an advertising agency for the
} Super-Duper-Triple-D Breast Enhancement Device.
}
} If you look at all the possible combinations and factor in whether each
} participant knowingly or unknowingly performed their roles, and whether
} they performed their part either knowingly and maliciously, or
} unknowingly, or because they were uneducated about the properties of their
} imaging devices, you can see that the images might or might not represent
} the "truth". And this is WITHOUT digital or even darkroom
} manipulation! Throw in differences in lighting and possibly the film type
} and even the chemicals used to make the prints, and you see that you do
} not have a controlled experiment here. Add a little personal bias (I might
} personally consider the model to be an airhead and the first boyfriend to
} be a fool and the second to be a power-hungry, manipulating character, and
} so might draw conclusions based on personal bias when viewing the
} images) and you've got not only flawed data, but a possible
} misinterpretation of the data.
}
} Now let's open these pictures in Photoshop ...
}
} Aloha,
} Tina
}
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Wed Jul 24 09:18:56 2002



From: Gary Gill :      garygill-at-dcla.com
Date: Wed, 24 Jul 2002 09:12:03 -0500
Subject: eyepiece graticule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


http://www.reticles.com/cckr810.htm
Klarmann Rulings Inc.
COMPARATOR
KR-810 360° PROTRACTOR

Gary Gill

-----Original Message-----
} From: "Eric.Hines%csiro.au"-at-sparc5.microscopy.com
[mailto:"Eric.Hines%csiro.au"-at-sparc5.microscopy.com]
Sent: Tuesday, July 23, 2002 6:24 PM
To: microscopy-at-sparc5.microscopy.com


Dear all,
Does anyone know of a supplier of eyepiece graticules with radial lines at
known angles (a protractor) so we can measure angles through the microscope.
Eric.


From daemon Wed Jul 24 09:30:04 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 24 Jul 2002 10:29:03 -0400
Subject: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;

Should the term "optical" be used at all when there is an optical to
electronic transformation such as in a photomultiplier tube in an SEM or a
CCD device in an electronic camera? I imagine if the word "optical" is used
there should be no transformation from light to electron and the path
between the human eye and the illuminated specimen should be direct other
than the glass in between. However, one must somehow get the "optical" image
onto some medium such as a piece of paper as one would do in "wet"
photography and emulsions. In the end, every image no matter how generated,
is a perception of reality in the human brain or for that matter any
specie's brain/nervous system. I might ask what is color in the mind? Is
blue still blue when my dog looks at it? I will check with my dog on this.

Peter

-----Original Message-----
} From: Gillmeister, Russ [mailto:RGillmeister-at-crt.xerox.com]
Sent: Tuesday, July 23, 2002 1:32 PM
To: 'Ian MacLaren'
Cc: 'MSA'


Ian,
I think it would be great if the term light microscopy were used.
TEM is definitely an optical microscopy technique.
You could argue about SEM. While the column is electron optical, the image
formation mechanism is not.
SPM is not with possible exception of near field.

My two cents. Or maybe one today.
The longer I work the farther I am from retirement!!!!
Russ Gillmeister
Xerox
~~~~~~~~~~~~~


-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Tuesday, July 23, 2002 8:05 AM
To: Microscopy-at-sparc5.microscopy.com


Dear all,
What do you consider to be the standard terminology for microscopy involving
a conventional microscope with the sample illuminated by light?

Optical microscopy?

Light Microscopy?

Light optical microscopy?

Something else?

The first seems to be often used, but as far as I can see, every form of
microscopy is optical, whether using light, electrons, X-rays or something
else. So, it seems to be a bit too vague.

What should I use then?

Best wishes

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Wed Jul 24 10:28:07 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 24 Jul 2002 11:05:34 -0400
Subject: Polaroid SprintScan45 drivers for Windows 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a polaroid SprintScan45 and we have just updated the computer which is running Windows 2000. Has anyone successfully got this scanner working with 16 bit mode? I would be interested in getting copies of the drivers that you are using. Please respond off the list. Thanks.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)




From daemon Wed Jul 24 10:32:19 2002



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Wed, 24 Jul 2002 11:26:19 -0400
Subject: Re: eyepiece graticule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Eric-
I noticed that Edmund Industrial Optics sells such an item for
$82.50US. Pg. 269 in their 2002 catalogue (800)363-1992.

Sincerely,
Matthew Ervin, Ph.D.
(301)394-0017 phone, (301)394-1559 fax
MErvin-at-ARL.Army.mil

M/S: AMSRL-SE-RL
US Army Research Laboratory
2800 Powder Mill Road
Adelphi, MD 20783-1197

Disclaimer: The opinions and views expressed above are those of the author
and do not necessarily represent those of the U.S. Army Research Laboratory
or any other government agency



"Eric.Hines-at-csiro.au"
To: microscopy-at-sparc5.microscopy.com
07/23/02 07:23 PM cc:
Subject: eyepiece graticule






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,
Does anyone know of a supplier of eyepiece graticules with radial lines at
known angles (a protractor) so we can measure angles through the
microscope.
Eric.







From daemon Wed Jul 24 10:32:36 2002



From: James.Passmore-at-sealedair.com
Date: Wed, 24 Jul 2002 11:27:38 -0400
Subject: Re: Software hunt update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Steve,

I tried a couple of searches on ZDNet downloads, and found several
shareware programs priced around $20.

Try this link:
http://downloads-zdnet.com.com/3120-20-0.html?qt=poster&tg=dl-2001
(You will have to weed out programs to post to Usenet newsgroups,
screensavers, etc., but there really aren't too many to deal with.)

HTH,
Jim Passmore
Sr. Analytical Chemist
Cryovac Division
Sealed Air Corporation
james.passmore-at-sealedair.com
864-433-2927 voice
864-433-2205 fax






|---------+----------------------------}
| | swiding |
| | {swiding-at-temple.e|
| | du} |
| | |
| | 07-24-02 08:15 AM|
| | |
|---------+----------------------------}
} --------------------------------------------------------------------------------------------------------------|
| |
| To: E-mail/ {Microscopy" {microscopy-at-sparc5.microscopy.com} "-at-nimbus.ocis.temple.edu} |
| cc: |
| Subject: Software hunt update |
} --------------------------------------------------------------------------------------------------------------|




------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello List,

Thanks for all the suggestions. What I am looking for is a graphics
program that will print banners on sheet paper (8.5 x 11). Our old program
would spread the banner across the sheets. After printing, you would put
the sheets together for the banner. We have Canvas, Photoshop, Paint Shop
Pro,
Powerpoint, etc. software but as far as I can determine none of them have
this feature.

Thanks,

Steve Widing
EM Tech / Computer Labs Manager
Biology Department
Temple University
Philadelphia, PA









From daemon Wed Jul 24 11:01:41 2002



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Wed, 24 Jul 2002 08:54:44 -0700
Subject: Re: Software hunt update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

Illustrator can spread the print over several 8.5 x 11 sheets and I would
expect that Canvas could also. It is called "tiling" so check the Help
files to see if it is listed. We use Illustrator and PowerPoint for
posters up to 44 inches by several feet. PowerPoint is limited to 52 x 52
inches but can be scaled. Illustrator costs $99 with an educational
discount at our bookstore and is the program of choice for posters, etc,
around here. We find some issues with the proprietary postscript in
PowerPoint but no problems with Illustrator.

Good luck,
Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
http://katie.ucdavis.edu
raharris-at-ucdavis.edu


At 08:15 AM 7/24/2002 -0400, swiding wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jul 24 11:17:21 2002



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Wed, 24 Jul 2002 12:10:38 -0400
Subject: Software hunt update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Steve,

Do you have access to Microsoft Publisher?? It has templates for banners.
Or, if you need to customize your own sizes, you can do so, and then it has
a print option called "tile". If your material is, say, 18 inches by 4 feet,
the "tile" feature will print it out across an appropriate number of 8.5x11
pages, complete with alignment markings for trimming and lining up the
output. Is this what you need??

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: swiding [mailto:swiding-at-temple.edu]
Sent: Wednesday, July 24, 2002 8:16 AM
To: E-mail


Hello List,

Thanks for all the suggestions. What I am looking for is a graphics
program that will print banners on sheet paper (8.5 x 11). Our old program
would spread the banner across the sheets. After printing, you would put
the sheets together for the banner. We have Canvas, Photoshop, Paint Shop
Pro,
Powerpoint, etc. software but as far as I can determine none of them have
this feature.

Thanks,

Steve Widing
EM Tech / Computer Labs Manager
Biology Department
Temple University
Philadelphia, PA



From daemon Wed Jul 24 11:47:40 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Wed, 24 Jul 2002 12:39:30 -0400
Subject: Re: Microscopy nomenclature

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Bob:
}
} Google search "photon microscopy" - 26000 articles http://www.google.com/search?hl=en&ie=UTF-8&oe=UTF-8&q=photon+microscopy
} Sergey

Sergey:

A caution on misinterpreting number of articles listings on google searches, and some general advice on constructing search strings to better eliminate "false hits", if I may.

It seems to me that two-photon, multi-photon, three-photon, entangled-photon and "photon emission microscopy" ought to be eliminated from your google search judging from the context of this thread (these all seem like much more specific terms than what listers are going after). Trying this google search instead:
"photon microscopy" -2-photon -entangled -two-photon -multi-photon -entangled
cuts your 26000 articles down to 34.

This could not be cut down with more search terms, because google limits search terms to 10 words, preventing me from eliminating "three-photon", "3-photon", "dual-photon", "single-photon", "photon emission microscopy"

Searches within those 34 pages yielded only 1 or 2 that I would say may be using the term "photon microscopy" as a general term like "light microscopy". Looks more like all of them are probably referring to various fluorescence microscopies.

Happy Googling! (how's that for terminology!)

-Kevin


Kevin Frischmann, Laboratory Manager
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: 212-313-7975
Fax: 212-496-3480
email: kfrisch-at-amnh.org



From daemon Wed Jul 24 12:39:37 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Wed, 24 Jul 2002 13:32:42 -0400
Subject: Software hunt update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

If you have Adobe Illustrator "page tiling" will solve your problem. Other programs with "banner" features include: QuarkXpress, PageMaker, and CorelDRAW!. A cheaper, more consumer-oriented alternative is Corel Print House. Also, printer driver features may help - look for "N-up" options (document options... page layout in Windows), which allow spreading a print job across 1x2, 2x2, 2x3, 3x3 & 4x4 pages in the drivers I have.


Kevin Frischmann, Laboratory Manager
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: (212) 313-7975
Fax: (212) 496-3480
email: kfrisch-at-amnh.org


************************************************************


} Hello List,
}
} Thanks for all the suggestions. What I am looking for is a graphics
} program that will print banners on sheet paper (8.5 x 11). Our old program
} would spread the banner across the sheets. After printing, you would put
} the sheets together for the banner. We have Canvas, Photoshop, Paint Shop Pro,
} Powerpoint, etc. software but as far as I can determine none of them have
} this feature.
}
} Thanks,
}
} Steve Widing
} EM Tech / Computer Labs Manager
} Biology Department
} Temple University
} Philadelphia, PA



From daemon Wed Jul 24 12:45:11 2002



From: Pierre-M. Charest :      pcharest-at-rsvs.ulaval.ca
Date: Wed, 24 Jul 2002 13:36:32 -0400
Subject: M&M 2002 - Golf Tournament & Social Events

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleague Microscopist

We still have some places available for the Golf Tournament of
Microscopy & Microanalysis 2002. Departure will be on Sunday August
4 at 9:00 AM in front of the Convention Centre of Quebec City.

If you will to register, please contact Jean-Marc Tremblay at the
following email address:

jmarc-at-qvc.qc.ca

Cost: $75/person includes Green Fee, Cart, Transportation,
Round-trip and refreshments.
Deadline: August 31

The Local Arrangement Committee (LAC) informs you that Option #1
(Firework Festival) for the Wednesday Night Social is sold out.
However, Option #2 (Music and Dinner at the Chapel of Amerique
Francaise) is still on sale. Tickets will be sold at the LAC booth
in the exhibit hall of the Convention Centre.

We look forward to seeing you in the Beautiful City of Quebec,

Pierre



From daemon Wed Jul 24 12:59:34 2002



From: Pierre-M. Charest :      pcharest-at-rsvs.ulaval.ca
Date: Wed, 24 Jul 2002 13:49:26 -0400
Subject: M&M 2002 - Golf Tournament & Social Events

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Dear Colleague Microscopist

We still have some places (22) available for the Golf Tournament of
Microscopy & Microanalysis 2002. Departure will be on Sunday August
4 at 9:00 AM in front of the Convention Centre of Quebec City.

If you will to register, please contact Jean-Marc Tremblay at the
following email address:

jmarc-at-qvc.qc.ca

Cost: $75/person includes Green Fee, Cart, Transportation,
Round-trip and refreshments.
Deadline Correction: JULY 31

The Local Arrangement Committee (LAC) informs you that Option #1
(Firework Festival) for the Wednesday Night Social is sold out.
However, Option #2 (Music and Dinner at the Chapel of Amerique
Francaise) is still on sale. Tickets will be sold at the LAC booth
in the exhibit hall of the Convention Centre.

We look forward to seeing you in the Beautiful City of Quebec,

Pierre



From daemon Wed Jul 24 14:19:36 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 24 Jul 2002 15:11:53 -0400
Subject: Electropolishing

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I remember using a Perchloric acid solution with Butyl Cellusolve, cooled on a very difficult cast Inconel 718 alloy. I bet that it would work for you.

Please read up on the safety precautions for Perchloric acid solutions.

Here are some recipes to start with. I don't have all the voltages and temperatures. Most of the perchloric solutions that I have used int he past have been chilled.

20%Perchloric acid
70%ethanol
10%butyl cellusolve

20% pechloric
80%ethanol
22V, 0ºC

10% pechloric
90%ethanol
15V, 0ºC

20%Perchloric acid
50%ethanol
15%butyl cellusolve
15%water
4ºC




-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Wednesday, July 24, 2002 9:34 AM
To: microscopy list


Dear all,
Any suggestions for the best electropolishing solution and conditions for a
Nickel-based superalloy (CMSX-4). We have a Struers Tenupol.

Thanks

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/



From daemon Wed Jul 24 14:31:20 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Wed, 24 Jul 2002 20:26:42 +0100
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
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Geoff
Only some light and electron microscopes can be defined as optical in
the sense that the
image is created using lenses.
The scanning electron microscope does not image an object using
lenses.
In the SEM the sole function of the lenses in the electron optical
column
is to create the electron probe with which the specimen is scanned.
Therefore, by your criterion the TEM would be an optical microscope,
but the SEM would not.
In an exactly analogous way, the functions of the lens optics in a
laser scanning confocal microscope
are to form the diffraction limited spot and to spatially filter the
light emitted from that spot. NOT to generate a 2-D image.
Your definition of "optical" would therefore also exclude LSM from
the class Optical Microscopes.
Personally, I don't think either exclusion is particularly useful,
particularly when we consider that many modern
microscopes are hybrids of optical and other technologies. Where does
STEM fit in? Does x-ray microscopy need to
use an x-ray lens to qualify as an optical technique? What is the
status of scanned probe microscopes that use
laser optics to sense probe movement? Should Near-field optical
microscopy be renamed Near-field light microscopy?

Chris

} Optical Microscopy: does that mean it uses a lens system to examine
an
} object? If so then it becomes impossible to separate the Electron
} Microscopes from Light microscopes.
}
} Here are a few terms I use:
} CLM: Conventional Light Microscopy (I learned from Dr. Joanne
Whallon
} and Dr Shirely Owens at Michigan Sate University)
} This has been shortened to just:
} LM: Light microscopy with the assumption made that the light is
between
} UV and IR
}
} And yes - I will get directly to the posed question so as to say on
} topic and focused to Ian MacLaren's original Email:
}
} Light Microscopy works very well and I think deserves the position
of
} being the 'correct' term. LM is a nice simple abbreviation that
goes
} very well with TEM, SEM, AFM, STEM, LSM, CLSM, LSCM (those confocal
} folks need to settle down and pick LSM ;) ).
}
} Make any sense?
}
} Geoff Williams
} Microscopy Facility Supervisor
} Biology Department
} Central Michigan University
} Mt Pleasant, MI 48859
}
}



From daemon Wed Jul 24 14:42:28 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Wed, 24 Jul 2002 15:35:59 -0400
Subject: Re: Software hunt update - my error

Contents Retrieved from Microscopy Listserver Archives
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} "...Also, printer driver features may help - look for "N-up" options (document options... page layout in Windows), which allow spreading a print job across 1x2, 2x2, 2x3, 3x3 & 4x4 pages in the drivers I have."

oops - forget that part; N-up is the opposite of what you want, but I believe I've seen print drivers that can accomplish what you want.

Kevin Frischmann, Laboratory Manager
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: 212-313-7975
Fax: 212-496-3480
email: kfrisch-at-amnh.org



From daemon Wed Jul 24 15:02:23 2002



From: Wang, Zhiyu :      Zhiyu_Wang-at-Maxtor.com
Date: Wed, 24 Jul 2002 13:56:11 -0600
Subject: FW: INCA deadtime problem

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Hi, Yuquan:

Recently I experienced a very similar problem. I got through the problems
and made this toy work.
Here is my status and solution:

System: Oxford Link ISIS 300 with ATW2 window 10 mm2, Hitachi S3500N VP SEM
, W-filament without airlock.
Problem on 03/25/02: Deadtime came to 100% suddenly, even without beam on.
The zero energy peak floated to 0.2-0.3 KeV, heavy background on 0.1-1 KeV.
The system was totally useless.
Solution: Run detector reconditioner program (takes 2.00 hours). Then shut
down EDS computer, and shut down EDS controller box. Then turn on the EDS
controller, and reboot the EDS computer and system. Use Co standard and
high vacuum mode, 25KV, acquire a EDS spectrum. High deadtime was gone.
Adjust beam current so that the count rate is ~1500 and deadtime is ~30%,
and close acquisition window (this step sets up your SEM to right beam dose
for EDS detector). Next, run Full Calibration program and sign Co as the
standard on dialog window. It takes about 5 minutes. Then calibrate the
spectrum for location of zero energy peak on acquisition window. All set up
is done. It is my feeling that the Oxford software has less intelligence to
clean up the junk program in the system and you have to totally shut down
(power off) the system to get rid of the junk program segments.

Problem on 07/19/02: The same problem came again, and the same procedure
was conducted. Problem is gone.

I have no clear clue what causes the high deadtime. If there is a pinhole
on window, the EDS functionality should not be able to recover and abnormal
LN2 consumption would be the result. The work load on this SEM is extremely
heavy, we switch between VP and high vacuum modes, use high voltages from
0.8-25KV at any value, change sample about 30 times/day. No air dry device
or dry N2 attached to the release air line. Icing and outgas product on
surface of window may be the most likely candidate for this problem. You
need to make sure there is no lighting source (chamber view source) and
radioactivity material involved during reconditioning and full calibration
procedure.

Hopeful this helps a bit.

Zhiyu Wang
Sr. Engineer
Maxtor Corp.
Milpitas, CA
(408)894-5588



} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Listers:
} } }
} } } The last year we installed ENCA 200 system in our JSM 840 SEM. Last
week
} } } when we repaired our SEM, liquid nitrogen in dewar was emptied to
remove
} } } water. Then we re-filled new liquid nitrogen through "warm up" and
"cool
} } } down" procedures. After that, we tried to calibrate this ISIS system.
When
} } } making "discriminators only", a message showed "ISIS calibration unable
to
} } } measure strobe peak. Abandoning calibration". At somebody's suggestion,
} } } several times of conditioner were conducted, but the situation is the
same.
} } } We couldn't do anything now. The system seems to be locked. Also when
} } } running EDS, no any peaks could be found, showing very high deadtime
(99%).
} } } Even without beam, the deadtime is also very high. It was suspected
that the
} } } detector system was damaged. I suspect that the strobed zero peak is
} } } generated in the XP2 pulse processor, not in the detector. Why
} } } "discriminators only" didn't work?
} } }
} } } If anybody of you has experience with this type of problems, I would
like to
} } } have your opinions and thoughts. Thanks!
} } }
} } } Our system No. 05426-2390-414-51, Model 6506, att 1: 32804c802, att2:
} } } 0120024, window: ATW2 det. area 10 mmxmm.
} } }
} } } Yuquan Ding
} } } Materials Labs
} } } Dept. of Mechanical Engineering
} } } University of Waterloo
} } } Waterloo, ON N2L 3G1
} } } 519-888-4567 x3766
} } } Fax: 519-888-6197
} } } Email: yding-at-uwaterloo.ca
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 23 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of
materials
} Computer applications and networking
}
}
}


From daemon Wed Jul 24 15:13:41 2002



From: robert.fowler-at-tdktca.com
Date: Wed, 24 Jul 2002 16:11:26 -0400
Subject: Grain Boundary Etch

Contents Retrieved from Microscopy Listserver Archives
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Hi people
I need a grain boundary etchant for ceramic chip capacitors. I would like
to view the ceramic boundaries. If someone out there that has a formula to
share I would appreciate. Thank you

p.s. I know this is not the best forum for this question but I have
received no response from EDFAS and hope that someone here or someone you
may know can answer this question.

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



From daemon Wed Jul 24 15:26:34 2002



From: Paul.Nolan-at-alcan.com
Date: Wed, 24 Jul 2002 16:19:24 -0400
Subject: microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We need to cut "thick?" cross sections of polymer/metal laminates for FTIR
microscope analysis.
Material thickness is around 100 microns..(section thickness somewhere
around 0.5 to 1mm i think)
I have 2 ultra microtomes but the sample size and section thickness
obtained from the ultramicrotome are to small for this application.

Any suggestions on the type of microtome i should consider. (Used is a
definite option)
or is there another piece of equipment i can use for this?

We are currently slicing pieces of our sample with a razor blade but this
causes some smearing in the layers.
I am currently being courted by one vendor who is going to lend me a
microtome to try out.

Suggestions are greatly appreciated
Vendors please reply to me directly.

Cheers

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



From daemon Wed Jul 24 15:30:07 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 24 Jul 2002 16:30:06 -0700
Subject: Licensing policies for use of photomicrographs

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I would like to know of any licensing policies and procedures used by
university EM and/or imaging facilities to handle requests to use
photomicrographs.
Thanks once again for your input.
Rosemary



From daemon Wed Jul 24 15:47:07 2002



From: Pollinger, Mel :      pollingm-at-bsci.com
Date: Wed, 24 Jul 2002 16:41:41 -0400
Subject: RE: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Geoff:

Which would you rather be in contact with, a high intensity beam of focused
light from a microscope lamp, or an electron beam of 50,000volts. I think
you are confused if you think there is no difference in IR, UV, visible
light, and electron microscopy. It seems that your desire is to win an
argument based upon silly logic, rather than reason and science.

Mel

} ----------
} From: Geoff Williams[SMTP:willi1gl-at-cmich.edu]
} Sent: Wednesday, July 24, 2002 9:33 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: Terminology: optical microscopy?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Lets pose a question:
}
} What is microscopy? The use of some form of the EMS to examine an
} object's properties.
}
} What are Optics? Are they not the component of the system that allows
} us to harness and control the particular range of the EMS we want to
} use?
}
} I don't agree that you can separate Optics as being only for the use of
} longer wavelength (Visual spectrum or say UV-IR).
}
} Optics should be as defined by physics. Any lens element that changes
} the character or distribution of any component of the EMS.
}
} I'm going through a small library behind me here are a few snippets:
}
} "The transmission electron microscope is a type of microscope and thus
} conforms to the definition of a microscope which is "an optical
} instrument consisting of a lens or a combination of lenses used for
} making enlarged or magnified images of minute objects.""
} -Clinton J. Dawes 'Biological Techniques for Transmission and Scanning
} Electron Microscopy 1979 edition published by Ladd. - third printing
}
} That's just one good example.
}
} Optical Microscopy: does that mean it uses a lens system to examine an
} object? If so then it becomes impossible to separate the Electron
} Microscopes from Light microscopes.
}
} Here are a few terms I use:
} CLM: Conventional Light Microscopy (I learned from Dr. Joanne Whallon
} and Dr Shirely Owens at Michigan Sate University)
} This has been shortened to just:
} LM: Light microscopy with the assumption made that the light is between
} UV and IR
}
} And yes - I will get directly to the posed question so as to say on
} topic and focused to Ian MacLaren's original Email:
}
} Light Microscopy works very well and I think deserves the position of
} being the 'correct' term. LM is a nice simple abbreviation that goes
} very well with TEM, SEM, AFM, STEM, LSM, CLSM, LSCM (those confocal
} folks need to settle down and pick LSM ;) ).
}
} Make any sense?
}
} Geoff Williams
} Microscopy Facility Supervisor
} Biology Department
} Central Michigan University
} Mt Pleasant, MI 48859
}
}


From daemon Wed Jul 24 16:05:22 2002



From: Gary Gill :      garygill-at-dcla.com
Date: Wed, 24 Jul 2002 09:12:03 -0500
Subject: eyepiece graticule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


http://www.reticles.com/cckr810.htm
Klarmann Rulings Inc.
COMPARATOR
KR-810 360° PROTRACTOR

Gary Gill

-----Original Message-----
} From: "Eric.Hines%csiro.au"-at-sparc5.microscopy.com
[mailto:"Eric.Hines%csiro.au"-at-sparc5.microscopy.com]
Sent: Tuesday, July 23, 2002 6:24 PM
To: microscopy-at-sparc5.microscopy.com


Dear all,
Does anyone know of a supplier of eyepiece graticules with radial lines at
known angles (a protractor) so we can measure angles through the microscope.
Eric.


From daemon Wed Jul 24 16:19:34 2002



From: Pollinger, Mel :      pollingm-at-bsci.com
Date: Wed, 24 Jul 2002 17:14:22 -0400
Subject: RE: eyepiece graticule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Edmund Scientific - check their website

} ----------
} From: Gary Gill[SMTP:garygill-at-dcla.com]
} Sent: Wednesday, July 24, 2002 10:12 AM
} To: '"Eric.Hines%csiro.au"-at-sparc5.microscopy.com';
} microscopy-at-sparc5.microscopy.com
} Subject: RE: eyepiece graticule
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} http://www.reticles.com/cckr810.htm
} Klarmann Rulings Inc.
} COMPARATOR
} KR-810 360° PROTRACTOR
}
} Gary Gill
}
} -----Original Message-----
} } From: "Eric.Hines%csiro.au"-at-sparc5.microscopy.com
} [mailto:"Eric.Hines%csiro.au"-at-sparc5.microscopy.com]
} Sent: Tuesday, July 23, 2002 6:24 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: eyepiece graticule
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} Does anyone know of a supplier of eyepiece graticules with radial lines at
} known angles (a protractor) so we can measure angles through the
} microscope.
} Eric.
}


From daemon Wed Jul 24 16:24:25 2002



From: Pollinger, Mel :      pollingm-at-bsci.com
Date: Wed, 24 Jul 2002 17:22:34 -0400
Subject: RE: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Um,

No argument. Not at all. I thought this was a discussion. Geesh.
Forgive me for thinking there would be reasonable discussion about this
like in other open forum/listserver stuff I'm a member of.

As for silly logic.

Does not logic follow that a detector is the final source? Confocal:
PMT or CCD (in general) is the detector. Still dependent on optics is
it not? IE to focus and scan the beam? Right?

SEM. Sure its just an electron microprobe, but it still uses the
fundamentals of optics to create that probe does it not?

No I'm not confused. I'm pretty darn clear. But maybe I'm making it
too simple. To clean.

Optical microscopy is pretty much all microscopy. Sure some just use a
focused beam of electrons to generate X-rays or to mill samples. Still
requires the use of optics.

I think too many people even in this community loose sight of
fundamental underlining interconnections.

I've no need to win arguments. I enjoy reading the comments.

Mel, I hate to say it but you sound like the person with silly logic
void of tangible related science ;)

Every type of microscope has a different application and use. Yes they
are all pretty much optical microscopes. When you think about them in
that manner and then begin to examine their differences the taxonomy
becomes clear and painfully simple. Maybe it is the fact that taxonomy
isn't unfamiliar to me as a Biologically trained microscopist.

Sorry Allen, your's wasn't quite the RIP. ;)

Geoff

-----Original Message-----
} From: Pollinger, Mel [mailto:pollingm-at-bsci.com]
Sent: Wednesday, July 24, 2002 4:42 PM
To: Microscopy-at-sparc5.microscopy.com; 'Geoff Williams'


Dear Geoff:

I can see that you must win the argument. So be it! You are the winner!

Mel

} ----------
} From: Geoff Williams[SMTP:willi1gl-at-cmich.edu]
} Sent: Wednesday, July 24, 2002 5:18 PM
} To: 'Pollinger, Mel'; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Terminology: optical microscopy?
}
} Um,
}
} No argument. Not at all. I thought this was a discussion. Geesh.
} Forgive me for thinking there would be reasonable discussion about this
} like in other open forum/listserver stuff I'm a member of.
}
} As for silly logic.
}
} Does not logic follow that a detector is the final source? Confocal:
} PMT or CCD (in general) is the detector. Still dependent on optics is
} it not? IE to focus and scan the beam? Right?
}
} SEM. Sure its just an electron microprobe, but it still uses the
} fundamentals of optics to create that probe does it not?
}
} No I'm not confused. I'm pretty darn clear. But maybe I'm making it
} too simple. To clean.
}
} Optical microscopy is pretty much all microscopy. Sure some just use a
} focused beam of electrons to generate X-rays or to mill samples. Still
} requires the use of optics.
}
} I think too many people even in this community loose sight of
} fundamental underlining interconnections.
}
} I've no need to win arguments. I enjoy reading the comments.
}
} Mel, I hate to say it but you sound like the person with silly logic
} void of tangible related science ;)
}
} Every type of microscope has a different application and use. Yes they
} are all pretty much optical microscopes. When you think about them in
} that manner and then begin to examine their differences the taxonomy
} becomes clear and painfully simple. Maybe it is the fact that taxonomy
} isn't unfamiliar to me as a Biologically trained microscopist.
}
} Sorry Allen, your's wasn't quite the RIP. ;)
}
} Geoff
}
} -----Original Message-----
} From: Pollinger, Mel [mailto:pollingm-at-bsci.com]
} Sent: Wednesday, July 24, 2002 4:42 PM
} To: Microscopy-at-sparc5.microscopy.com; 'Geoff Williams'
} Subject: RE: Terminology: optical microscopy?
} Importance: High
}
} Dear Geoff:
}
} Which would you rather be in contact with, a high intensity beam of
} focused
} light from a microscope lamp, or an electron beam of 50,000volts. I
} think
} you are confused if you think there is no difference in IR, UV, visible
} light, and electron microscopy. It seems that your desire is to win an
} argument based upon silly logic, rather than reason and science.
}
} Mel
}
} } ----------
} } From: Geoff Williams[SMTP:willi1gl-at-cmich.edu]
} } Sent: Wednesday, July 24, 2002 9:33 AM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: RE: Terminology: optical microscopy?
} }
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} }
} } Lets pose a question:
} }
} } What is microscopy? The use of some form of the EMS to examine an
} } object's properties.
} }
} } What are Optics? Are they not the component of the system that allows
} } us to harness and control the particular range of the EMS we want to
} } use?
} }
} } I don't agree that you can separate Optics as being only for the use
} of
} } longer wavelength (Visual spectrum or say UV-IR).
} }
} } Optics should be as defined by physics. Any lens element that changes
} } the character or distribution of any component of the EMS.
} }
} } I'm going through a small library behind me here are a few snippets:
} }
} } "The transmission electron microscope is a type of microscope and thus
} } conforms to the definition of a microscope which is "an optical
} } instrument consisting of a lens or a combination of lenses used for
} } making enlarged or magnified images of minute objects.""
} } -Clinton J. Dawes 'Biological Techniques for Transmission and Scanning
} } Electron Microscopy 1979 edition published by Ladd. - third printing
} }
} } That's just one good example.
} }
} } Optical Microscopy: does that mean it uses a lens system to examine an
} } object? If so then it becomes impossible to separate the Electron
} } Microscopes from Light microscopes.
} }
} } Here are a few terms I use:
} } CLM: Conventional Light Microscopy (I learned from Dr. Joanne Whallon
} } and Dr Shirely Owens at Michigan Sate University)
} } This has been shortened to just:
} } LM: Light microscopy with the assumption made that the light is
} between
} } UV and IR
} }
} } And yes - I will get directly to the posed question so as to say on
} } topic and focused to Ian MacLaren's original Email:
} }
} } Light Microscopy works very well and I think deserves the position of
} } being the 'correct' term. LM is a nice simple abbreviation that goes
} } very well with TEM, SEM, AFM, STEM, LSM, CLSM, LSCM (those confocal
} } folks need to settle down and pick LSM ;) ).
} }
} } Make any sense?
} }
} } Geoff Williams
} } Microscopy Facility Supervisor
} } Biology Department
} } Central Michigan University
} } Mt Pleasant, MI 48859
} }
} }
}


From daemon Wed Jul 24 18:05:08 2002



From: microscopy-at-microscopy.com
Date: Wed, 24 Jul 2002 18:57:20 -0500
Subject: Mother finds 71 Thousand in 15 Year Olds Closet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All our mailings are sent complying to the proposed H.R. 3113 Unsolicited Commercial Electronic Mail Act of 2000. Please see the bottom of this message for further information and removal instructions.


PARENTS OF 15 - YEAR OLD - FIND $71,000 CASH HIDDEN IN
HIS CLOSET!

Does this headline look familiar? Of course it does. You most likely have just
seen this story recently featured on a major nightly news program (USA).
And reported elsewhere in the world (including my neck of the woods –
New Zealand). His mother was cleaning and putting laundry away when
she came across a large brown paper bag that was suspiciously buried
beneath some clothes and a skateboard in the back of her 15-year-old sons
closet. Nothing could have prepared her for the shock she got when she
opened the bag and found it was full of cash. Five-dollar bills, twenties,
fifties and hundreds - all neatly rubber-banded in labelled piles.

"My first thought was that he had robbed a bank", says
the 41-year-old woman, "There was over $71,000 dollars in that bag --
that's more than my husband earns in a year".

The woman immediately called her husband at the
car-dealership where he worked to tell him what she had discovered.He came
home right away and they drove together to the boys school and picked him up.
Little did they suspect that where the money came from was more shocking than
actually finding it in the closet.

As it turns out, the boy had been sending out, via
E-mail, a type of "Report" to E-mail addresses that he obtained off the
Internet. Everyday after school for the past 2 months, he had been doing
this right on his computer in his bedroom.

"I just got the E-mail one day and I figured what the
heck, I put my name on it like the instructions said and I started sending it
out", says the clever 15-year-old.

The E-mail letter listed 5 addresses and contained
instructions to send one $5 dollar bill to each person on the list, then delete
the address at the top and move the others addresses Down , and finally
to add your name to the top of the list.

The letter goes on to state that you would receive
several thousand dollars in five-dollar bills within 2 weeks if you sent out
the letter with your name at the top of the 5-address list. "I get junk
E-mail all the time, and really did not think it was going to work", the boy
continues.

Within the first few days of sending out the E-mail,
the Post Office Box that his parents had gotten him for his video-game
magazine subscriptions began to fill up with not magazines, but envelopes
containing $5 bills.

"About a week later I rode [my bike] down to the post office and my box
had 1 magazine and about 300 envelops stuffed in it. There was also a yellow
slip that said I had to go up to the [post office] counter.
I thought I was in trouble or something (laughs)". He goes on, "I went up
to the counter and they had a whole box of more mail for me. I had to ride
back home and empty out my backpack because I could not carry it all".
Over the next few weeks, the boy continued sending out the E-mail."The
money just kept coming in and I just kept sorting it and stashing it in the
closet, barely had time for my homework".He had also been riding his bike
to several of the banks in his area and exchanging the $5 bills for twenties,
fifties and hundreds.

"I didn't want the banks to get suspicious so I kept riding to different banks
with like five thousand at a time in my backpack. I would usually tell the lady
at the bank counter that my dad had sent me in to exchange the money] and
he was outside waiting for me.One time the lady gave me a really strange look
and told me that she would not be able to do it for me and my dad would have
to come in and do it, but I just rode to the next bank down the street (laughs)."
Surprisingly, the boy did not have any reason to be afraid.The reporting news

team examined and investigated the so-called "chain-letter" the boy was
sending out and found that it was not a chain-letter at all.In fact, it was
completely legal according to US Postal and Lottery Laws, Title 18,
Section 1302 and 1341, or Title 18, Section 3005 in the US code, also in the
code of federal regulations, Volume 16, Sections 255 and 436, which
state a product or service must be exchanged for money received.

Every five-dollar bill that he received contained a little note that read,
"Please send me report number XYX".This simple note made the letter
legal because he was exchanging a service (A Report on how-to) for
a five-dollar fee.

[This is the end of the media release. If you would
like to understand how the system works and get your $71,000 - please
continue reading. What appears below is what the 15 year old was sending out
on the net - YOU CAN USE IT TOO - just follow the simple instructions].

+++++++++++++++++++++++++++++++++++++++++++++++++
BE FINANCIALLY FREE LIKE OTHERS WITHIN A YEAR!!! Before
you say "Bull", please read the following. This is the letter you
have been hearing about on the news lately. Due to the popularity of
this letter on the Internet, a national weekly news program recently
devoted an entire show to the investigation of this program described
below, to see if it really can make people money. The show also
investigated whether or not the program was legal. Their findings
proved once and for all that there are "absolutely NO Laws prohibiting
the participation in the program and if people can follow the simple
instructions, they are bound to make some megabucks with only $25
out of pocket cost".


DUE TO THE RECENT INCREASE OF POPULARITY &
RESPECT THIS PROGRAM HAS ATTAINED, IT IS CURRENTLY
WORKING BETTER THAN EVER.


Note* follow the directons below, I had best results the second
time when i hired a bulk email service in addition to following the
reports instructions.

In order for all of us to be successful, many, many emails must be
sent so that the returns are many. I have been extremely successful
using the following company. They send out the offers, and all
I do is accept money for reports, then I send back to the people as
soon as possible.


This is what one had to say: "Thanks to this profitable
opportunity. I was approached many times before but each
time I passed on it. I am so glad I finally joined just to
see what one could expect in return for the minimal effort
and money required. To my astonishment, I received total
$610,470.00 in 21 weeks, with money still coming in".

Pam Hedland, Fort Lee, New Jersey.

+++++++++++++++++++++++++++++++++++++++++++++++++
Here is another testimonial: "This program has been around
for a long time but I never believed in it. But one day when
I received this again in the mail I decided to gamble my $25
on it. I followed the simple instructions and walaa ..... 3
weeks later the money started to come in. First month I
only made $240.00 but the next 2 months after that I made a
total of $290,000.00. So far, in the past 8 months by re-
entering the program, I have made over $710,000.00 and I am
playing it again. The key to success in this program is to
follow the simple steps and NOT change anything."
More testimonials later but first,


=

=For each report, send $5 CASH, THE NAME & NUMBER OF THE
REPORT YOU ARE ORDERING and YOUR E-MAIL ADDRESS to the
person whose name appears ON THAT LIST next to the report.
MAKE SURE YOUR RETURN ADDRESS IS ON YOUR ENVELOPE
TOP LEFT CORNER in case of any mail problems.

=You will need all 5 reports so that you can save them on your computer.
Within a few days you will receive, vie e-mail, each of the 5 reports from
these 5 different individuals. Save them on your computer so they will be
accessible for you to send to the 1,000's of people who will order them from
you. Also make a floppy of these reports and keep it on your desk in case
something happens to your computer.


IMPORTANT - DO NOT alter the names of the people who are
listed next to each report, or their sequence on the list, in any way other
than what is instructed below in step "1 through 6" or you will loose out
on majority of your profits. Once you understand the way this works, you
will also see how it does not work if you change it. Remember, this method
has been tested, and if you alter, it will NOT work!!! People have tried to
put their friends/relatives names on all five thinking they could get all the
money. But it does not work this way. Believe us, we all have tried to
be greedy and then nothing happened. So Do Not try to change anything
other than what is instructed. Because if you do, it will not work for you.
Remember, honesty reaps the reward!!!


1.... After you have ordered all 5 reports, take this
advertisement and REMOVE the name & address of the person in
REPORT # 5. This person has made it through the cycle and is
no doubt counting their fortune.
2.... Move the name & address in REPORT # 4 down TO REPORT #5.
3.... Move the name & address in REPORT # 3 down TO REPORT #4.
4.... Move the name & address in REPORT # 2 down TO REPORT #3.
5.... Move the name & address in REPORT # 1 down TO REPORT #2
6.... Insert YOUR name & address in the REPORT # 1 Position.


PLEASE MAKE SURE you copy every name & address ACCURATELY!
+++++++++++++++++++++++++++++++++++++++++++++++++


**** Take this entire letter, with the modified list of
names, and save it on your computer. DO NOT MAKE ANY OTHER
CHANGES. Save this on a disk as well just in case if you
loose any data. To assist you with marketing your business
on the internet, the 5 reports you purchase will provide you
with invaluable marketing information which includes how to
send bulk e-mails legally, where to find thousands of free
classified ads and much more. There are 2 Primary methods to
get this venture going:


METHOD #1: BY SENDING BULK E-MAIL LEGALLY
+++++++++++++++++++++++++++++++++++++++++++++++++


Let's say that you decide to start small, just to see how it
goes, and we will assume You and those involved send out
only 5,000e-mails each. Let's also assume that the mailing
receive only a 0.2% response (the response could be much
better but lets just say it is only 0.2%. Also many people
will send out hundreds of thousands e-mails instead of only
5,000 each). Continuing with this example, you send out only
5,000 e-mails.


With a 0.2% response, that is only 10 orders for report # 1.
Those 10 people responded by sending out 5,000 e-mail each
for a total of 50,000. Out of those 50,000 e-mails only 0.2%
responded with orders. That equals 100 people responded and
ordered Report # 2.

Those 100 people mail out 5,000 e-mails each for a total of
500,000 e-mails. The 0.2% response to that is 1000 orders
for Report #3.

Those 1000 people send out 5,000 e-mails each for a total of
5 million e-mails sent out. The 0.2% response to that is
10,000 orders for Report #4.

Those 10,000 people send out 5,000 e-mails each for a total
of 50,000,000 (50 million) e-mails. The 0.2% response to
that is 100,000 orders for Report #5. THAT'S 100,000 ORDERS
TIMES $5 EACH=$500,000.00 (half million).
Your total income in this example is: 1..... $50 +2.....
$500 + 3.....$5,000 + 4..... $50,000 + 5.....
$500,000........Grand Total=$555,550.00


NUMBERS DO NOT LIE. GET A PENCIL & PAPER AND FIGURE OUT THE
WORST POSSIBLE RESPONSES AND NO MATTER HOW YOU CALCULATE IT,
YOU WILL STILL MAKE A LOT OF MONEY!


+++++++++++++++++++++++++++++++++++++++++++++++++
REMEMBER FRIEND, THIS IS ASSUMING ONLY 10 PEOPLE ORDERING
OUT OF 5,000 YOU MAILED TO. Dare to think for a moment what
would happen if everyone or half or even one 4th of those
people mailed 100,000e-mails each or more? There are over
150 million people on the Internet worldwide and counting.
Believe me, many people will do just that, and more!


METHOD #2: BY PLACING FREE ADS ON THE INTERNET
+++++++++++++++++++++++++++++++++++++++++++++++++
Advertising on the net is very very inexpensive and there
are hundreds of FREE places to advertise. Placing a lot of
free ads on the Internet will easily get a larger response.


We strongly suggest you start with Method #1 and add METHOD
#2 as you go along. For every $5 you receive, all you must
do is e-mail them the Report they ordered. That's it. Always
provide same day service on all orders. This will guarantee
that the e-mail they send out with your name and address on
it, will be prompt because they can not advertise until they
receive the report.


=ORDER EACH REPORT BY ITS NUMBER & NAME ONLY. Notes: Always
send $5 cash (U.S. CURRENCY) for each Report. Checks NOT
accepted. Make sure the cash is concealed by wrapping it in
at least 2 sheets of paper or aluminum foil. On one of those
sheets of paper, Write the NUMBER & the NAME of the Report
you are ordering, YOUR E-MAIL ADDRESS and your name and
postal address.

PLACE YOUR ORDER FOR THESE REPORTS NOW:

+++++++++++++++++++++++++++++++++++++++++++++++++
REPORT #1: The Insider's Guide to Advertising for Free on the Net


Order Report #1 from:

M. Eiseman
P.O.Box 451971
Sunrise, FL 33345-1971

__________________________________________________________
REPORT #2: The Insider's Guide to Sending Bulk e-mail on the Net


Order Report #2 from:

GM Boland
353 Jonestown Rd
Winston-Salem, N. C. 27104

__________________________________________________________
REPORT #3: Secret to Multilevel marketing on the Net


Order Report #3 from:

Mrs. Caroline Abee
113 Ervin Ave NE
Valdese, NC 28690-9601

__________________________________________________________
REPORT #4: How to become a millionaire utilizing MLM & the Net


Order Report #4 from:

L. Samon
P. O. Box 31
Castletown
Isle of Man
IM99 5XP

______________________________________________________
REPORT #5: How to send out 0ne Million emails for free


Order Report #5 From:

Gail R. Gitlitz
905 Belltown Rd.
Tellico Plns, Tn 37385

+++++++++++++++++++++++++++++++++++++++++++++++++

$$$$$$$$$$$$$$$$ YOUR SUCCESS GUIDELINES $$$$$$$$$$$$$$$$

Follow these guidelines to guarantee your success:

=within 2 weeks, continue sending e-mails until you do. =After you have received 10 orders, 2 to 3 weeks after that
you should receive 100 orders or more for REPORT #2. If you
did not, continue advertising or sending e-mails until you
do.

=YOU CAN RELAX, because the system is already working for
you, and the cash will continue to roll in! THIS IS
IMPORTANT TO REMEMBER: Every time your name is moved down on
the list, you are placed in front of a Different report.
You can KEEP TRACK of your PROGRESS by watching which report
people are ordering from you. IF YOU WANT TO GENERATE MORE
INCOME SEND ANOTHER BATCH OF E-MAILS AND START THE WHOLE
PROCESS AGAIN. There is NO LIMIT to the income you can
generate from this business!!!

+++++++++++++++++++++++++++++++++++++++++++++++++
FOLLOWING IS A NOTE FROM THE ORIGINATOR OF THIS PROGRAM: You
have just received information that can give you financial
freedom for the rest of your life, with NO RISK and JUST A
LITTLE BIT OF EFFORT. You can make more money in the next
few weeks and months than you have ever imagined. Follow the
program EXACTLY AS INSTRUCTED. Do Not change it in any way.
It works exceedingly well as it is now.

Remember to e-mail a copy of this exciting report after you
have put your name and address in Report#1 and moved others
to #2 thru #5 as instructed above. One of the people you
send this to may send out 100,000 or more e-mails and your
name will be on every one of them. Remember though, the
more you send out the more potential customers you will
reach. So my friend, I have given you the ideas,
information, materials and opportunity to become financially
independent. IT IS UP TO YOU NOW!

="My name is Mitchell. My wife, Jody and I live in Chicago. I
am an accountant with a major U.S. Corporation and I make
pretty good money. When I received this program I grumbled
to Jody about receiving "junk mail". I made fun of the whole
thing, spouting my knowledge of the population and
percentages involved. I "knew" it wouldn't work. Jody
totally ignored my supposed intelligence and few days later
she jumped in with both feet. I made merciless fun of her,
and was ready to lay the old "I told you so" on her when the
thing didn't work. Well, the laugh was on me! Within 3 weeks
she had received 50 responses. Within the next 45 days she
had received total $ 147,200.00....all cash! I was shocked.
I have joined Jody in her "hobby".

Mitchell Wolf, Chicago, Illinois

+++++++++++++++++++++++++++++++++++++++++++++++++
"Not being the gambling type, it took me several weeks to
make up my mind to participate in this plan. But
conservative that I am, I decided that the initial
investment was so little that there was just no way that I
wouldn't get enough orders to at least get my money back".
"I was surprised when I found my medium size post office box
crammed with orders. I made $319,210.00 in the first 12
weeks. The nice thing about this deal is that it does not
matter where people live. There simply isn't a better
investment with a faster return and so big".

Dan Sondstrom, Alberta, Canada

+++++++++++++++++++++++++++++++++++++++++++++++++
"I had received this program before. I deleted it, but later
I wondered if I should have given it a try. Of course, I had
no idea who to contact to get another copy, so I had to wait
until I was e-mailed again by someone else......11 months
passed then it luckily came again...... I did not delete
this one! I made more than $490,000 on my first try and all
the money came within 22 weeks".

Susan De Suza, New York, N.Y

+++++++++++++++++++++++++++++++++++++++++++++++++
If you have any questions of the legality of this program,
contact the Office of Associate Director for Marketing
Practices, Federal Trade Commission, Bureau of Consumer
Protection, Washington,D.C.
+++++++++++++++++++++++++++++++++++++++++++++++++

This email was sent to you via Saf-E Mail Systems. Your email address was automatically inserted into the To and From addresses to eliminate undeliverables which waste bandwidth and cause internet congestion. Your email or webserver IS NOT being used for the sending of this mail. No-one else is receiving emails from your address. You may utilize the removal link below if you do not wish to receive this mailing.

http://202.105.49.100/remove.html



From daemon Wed Jul 24 18:05:14 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 24 Jul 2002 19:05:02 -0400
Subject: Imaging Micro-organisms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;

Since many of you do biological imaging vis-a-vis SEM and "optical" means, I
have a question. When imaging bacteria or any micro-organism, is the species
identified by it's shape, general apperance, size etc.? Or better yet, just
how is it identified in the EM? Or does this require some other assay for
identification and then it is imaged to generate other information? I don't
image bio materials but it is something that I would like to be able to show
my daughter who is interested in the micro-biological world. I see a future
in electronic bio-identification and since her interests seem to lie in that
direction, I'd like to explain some of this intelligently.

Peter




From daemon Wed Jul 24 18:22:13 2002



From: Ray D. Twesten :      twesten-at-mrl.uiuc.edu
Date: Wed, 24 Jul 2002 18:13:32 -0500
Subject: Out of Office AutoReply: Mother finds 71 Thousand in 15 Year Olds Closet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ray Twesten will be away from the office until Monday 7/29/02.


From daemon Wed Jul 24 19:06:11 2002



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 24 Jul 2002 19:58:01 -0400 (EDT)
Subject: Re: microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have had great success cutting thick sections 100-500 nm (0.1-0.5 mm)
of biological samples using a Tissue Slicer from EMS. Vibratome is another
brand; not sure who sells it.

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Jul 24 19:07:21 2002



From: eckhard.langer-at-amd.com
Date: Thu, 25 Jul 2002 02:01:46 +0200
Subject: Out of Office AutoReply: Mother finds 71 Thousand in 15 Year

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm out of the office until 26.07.2002. Please contact MA Meyer or Malwine Cante.

Regards

Eckhard Langer



From daemon Wed Jul 24 19:39:54 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 24 Jul 2002 17:38:18 -0700
Subject: RE: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What difference does this make?

i.e., what is the purpose of this effort?
I think that the image manipulation topic is much
more important than this one. But that is MHO.

gary g.



From daemon Wed Jul 24 20:49:14 2002



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil (by way of
Date: Wed, 24 Jul 2002 20:38:41 -0500
Subject: Re: Software hunt - Canvas will work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Steve Widing,
Canvas will print on multiple sheets which can be taped or glued together to
make banners or posters. You need to set the document size to larger than one
sheet of paper. Select Page Setup in the File menu, select the Canvas 7 (or
Canvas 5) opinion, and click the "tile" button. This will set up
Canvas to print
across multiple sheets which are tiled to match your graphics file.
You can tape
these sheets together to form your banner. Good luck.
Tyrone Daulton

swiding wrote:

}
}
} Hello List,
}
} Thanks for all the suggestions. What I am looking for is a graphics
} program that will print banners on sheet paper (8.5 x 11). Our old program
} would spread the banner across the sheets. After printing, you would put
} the sheets together for the banner. We have Canvas, Photoshop,
} Paint Shop Pro,
} Powerpoint, etc. software but as far as I can determine none of them have
} this feature.
}
} Thanks,
}
} Steve Widing
} EM Tech / Computer Labs Manager
} Biology Department
} Temple University
} Philadelphia, PA

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Facility
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu


From daemon Wed Jul 24 20:49:15 2002



From: Nakama, Mari :      MNakama-at-feico.com (by way of MicroscopyListserver)
Date: Wed, 24 Jul 2002 20:38:50 -0500
Subject: Listserve Posting from FEI Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




This message is in response to a Listserver posting concerning Veeco
and FEI Merger.
-----------------------------------------------------------
Since we announced our proposed merger with Veeco Instruments last
Friday, July 12, some of you have raised concern over our future
commitment to your needs. FEI has grown as a result of our strong
technology portfolio and our ability to leverage it across our
diverse customer base, including the very important scientific
research sector. The solutions we offer cross over our industry
sectors more every year. Make no mistake, we intend to continue the
growth of Veeco FEI through expanded focus on the needs of the
scientific research industry and microscopists, delivering the tools
you need to succeed. Please visit our website at www.feicompany.com
{ {http://www.feicompany.com} http://www.feicompany.com} and visit the
merger information page that is accessible from our home page for
more on how we intend to serve you better as a result of this merger.

Thank you,
Vahe' Sarkissian,
Chairman, president and CEO, FEI Company.



Cheers,
George Scholes
Director of North American Sales
7451 NW Evergreen Parkway
Hillsboro, OR 97124
PH # 503-640-7644
FX # 503-640-7663
gscholes-at-feico.com


From daemon Wed Jul 24 20:49:15 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 24 Jul 2002 18:53:08 -0700
Subject: Re: Imaging Micro-organisms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter,

Microbes are like people and come in different sizes, shapes and
morphologies but can not be identified to the genus/species level
(such as Salmonella typhimurium) using EM alone. EM will allow one to
identify the microbe to the major group, however.

For example, the major categories of bacteria are Gram positive, Gram
negative. EM (as well as light microscopy after using Gram staining
procedures) will permit one to ID the bacteria as G+ or G- based on
cell wall structure. Then, based on the size and shape of the cell
(coccus, rod, curved, pleomorphic, etc.), the presence of various
structures (flagella, oil bodies, extracellular capsules, etc.) one
would probably get a "reasonable idea" of the genus (Streptococcus,
Bacillus, Vibrio, etc.) but that's about all one can achieve using
morphology alone.

Further biochemical (physiological) tests would be needed after
isolating the organism and growing a pure culture of it. These are
standard, microbiological tests and commercial kits are available,
including immunological and molecular ones.

Fungi would be identified in a similar manner (morphology, staining
reactions in LM, isolation of organism, biochemical/physiological
tests, etc.).

Viruses could be identified to the major family based on morphology
(herpes, adenovirus, poxvirus, etc.) and some viruses are so unique
(rabies, Ebola, Hepatitis B) that the morphology (and clinical
presentation) would give a fairly accurate "presumptive diagnosis"
that could later be confirmed by more specific tests (immunological,
molecular, etc.). An experienced medical microbiologist or
pathologist can give an amazingly accurate identification based on
morphology and clinical symptoms alone.

Of course, in the case of a newly emerged virus (where no test
systems have been developed) EM is extremely valuable and may give
the first indication that we are dealing with a new organism (Ebola,
Hepatitis B, HIV).

I hope that the microbiologically-oriented microscopists/pathologists
who follow this list will comment further on this matter based on
their own clinical and laboratory experiences dealing with a variety
of micro organisms. Needless to say, this is an extremely interesting
and potentially life-saving activity.

John B.


} From: Peter Tomic {PTomic-at-anadigics.com}
To: MSA listserver {Microscopy-at-sparc5.microscopy.com}


It is and it isn't. There are rod bacteria, spiral bacteria,
and other sorts of bacteria that do allow identification by
general appearance. I've have major discussions with
Tina Swatch about morphology differences between
similar bacteriums.

It seems that the bottom line is that you can distinguish the
major types by rod, sphere, spiral, etc. But to make a
determination of a specific type within one of these groups
is difficult. For example, Yersinia pestis and Yersinia enterocolitica
look basically the same. Pestis is the cause of the Plague. To me,
side by side, they look the same. E. coli, Salmonella and Listeria
kinda look the same too. But I'm not a microbio guy. So maybe
they would have a sweet spot in the microbio's eye.

If you want to find out what a specific bacterium is, that leads
to serology. That is way out of my league. I too look for
morphology. I'm most interested in whether the thing looks
like a rod or a spiral. And I want to know if this rod looks like
some other bacterium rod. Other than that, it is academic. And it is.

But for your daughter, the initial shapes should be of importance.
Rod, cocci, spiral, etc. These are well-documented. It is a very
big, tiny world. Marvelous....and potentially deadly.

For a good reference, try Black, J. (1996). Microbiology: Principles
& applications. Prentice-Hall: New Jersey. ISBN 0-13-190745-X

If she can get through this text, she should be able to get through
microbio 1A with no sweat. I sweat every time I read this book. There
is a lot there. Very good. It is sort of like a compendium of Widlar,
Grebene, Hunter, Meyer, Hamilton, Lynn, and Gray. But these are
not microbio. :-)

gary g.



At 04:05 PM 7/24/2002, you wrote:

} Folks;
}
} Since many of you do biological imaging vis-a-vis SEM and "optical" means, I
} have a question. When imaging bacteria or any micro-organism, is the species
} identified by it's shape, general apperance, size etc.? Or better yet, just
} how is it identified in the EM? Or does this require some other assay for
} identification and then it is imaged to generate other information? I don't
} image bio materials but it is something that I would like to be able to show
} my daughter who is interested in the micro-biological world. I see a future
} in electronic bio-identification and since her interests seem to lie in that
} direction, I'd like to explain some of this intelligently.
}
} Peter



From daemon Wed Jul 24 21:07:06 2002



From: =?windows-1252?Q?S=F8rensen_Henning_Sund?= :      Henning.S-at-danfoss.com
Date: Thu, 25 Jul 2002 04:00:47 +0200
Subject: Autosvar - Ikke til stede: Mother finds 71 Thousand in 15 Year Ol

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Jeg er tilbage på kontoret mandag d.29. juli 2002.
I am out of the office until Monday Juli 29, 2002.

Med venlig hilsen/Best regards
Henning Sund Sørensen
L7-S40/Tlf.:2309



From daemon Wed Jul 24 21:09:17 2002



From: Agneta =?iso-8859-1?Q?=D6stberg?= :      agneta.ostberg-at-sandvik.com
Date: Thu, 25 Jul 2002 03:59:56 +0200
Subject: Agneta =?iso-8859-1?Q?=D6stberg=2F0140=2FSANDVIK_is_out_of_the_office=2E?=

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office starting 2002-07-08 and will not return until
2002-08-05.

I will respond to your message when I return. If urgent contact Mette
Ramberg/0140/SANDVIK or Claes-Ove Pettersson/0140/SANDVIK.



From daemon Wed Jul 24 21:24:41 2002



From: zaluzec-at-microscopy.com
Date: Wed, 24 Jul 2002 21:14:56 -0500
Subject: Adminsitrivia: Out of Office replies....

Contents Retrieved from Microscopy Listserver Archives
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Yes folks I see them.

I'm trying to figure out how to effectively block them, all
of which started with a spam mail about "Mother finding 71 ...."

Now I hope you all understand why I ask people to unsubscribe rather
than just turning on an " out of the office message" ! I've been
able to stop these in the past, but the characteristics of
this set are unique and are get through the junk mail filter.

sigh...

Nestor


From daemon Wed Jul 24 21:30:45 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 24 Jul 2002 19:29:52 -0700
Subject: Re: Software hunt update

Contents Retrieved from Microscopy Listserver Archives
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This is a bleed issue. Most all programs can handle this.

There are many apps out there that can do what you want
and more. The specific ways of doing this will vary greatly.

Check it out from all aspects.

gary g.


At 05:15 AM 7/24/2002, you wrote:

} Hello List,
}
} Thanks for all the suggestions. What I am looking for is a graphics
} program that will print banners on sheet paper (8.5 x 11). Our old program
} would spread the banner across the sheets. After printing, you would put
} the sheets together for the banner. We have Canvas, Photoshop, Paint Shop Pro,
} Powerpoint, etc. software but as far as I can determine none of them have
} this feature.
}
} Thanks,
}
} Steve Widing
} EM Tech / Computer Labs Manager
} Biology Department
} Temple University
} Philadelphia, PA



From daemon Wed Jul 24 21:34:02 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 24 Jul 2002 19:33:13 -0700
Subject: Re: Grain Boundary Etch

Contents Retrieved from Microscopy Listserver Archives
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Why not just backscatter this image? There would not
be any special specimen prep or other efforts.

This is done all the time for FA of passive and active
components.

gary g.


At 01:11 PM 7/24/2002, you wrote:

} Hi people
} I need a grain boundary etchant for ceramic chip capacitors. I would like
} to view the ceramic boundaries. If someone out there that has a formula to
} share I would appreciate. Thank you
}
} p.s. I know this is not the best forum for this question but I have
} received no response from EDFAS and hope that someone here or someone you
} may know can answer this question.
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com



From daemon Wed Jul 24 23:49:01 2002



From: System Attendant :      ZMAIL-SA-at-zilog.com
Date: Wed, 24 Jul 2002 22:38:58 -0600
Subject: ScanMail Message: To Sender, sensitive content found and action t

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Trend SMEX Content Filter has detected sensitive content.

Place = microscopy-at-microscopy.com; ; ; microscopy-at-sparc5.microscopy.com
Sender = microscopy-at-sparc5.microscopy.com
Subject = Mother finds 71 Thousand in 15 Year Olds Closet
Delivery Time = July 24, 2002 (Wednesday) 22:38:56
Policy = Anti-Spam
Action on this mail = Quarantine message

Warning message from administrator:
Content filter has detected a sensitive e-mail.


From daemon Thu Jul 25 00:48:46 2002



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 25 Jul 2002 00:44:00 -0700
Subject: RE: Listserve Posting from FEI Company

Contents Retrieved from Microscopy Listserver Archives
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Thank you, Mari, for passing that belated message on.

I do hope that Veeco FEI breaks the mold of past mergers. This letter and
what is available on the web site is certainly more than past events have
offered. The only thing that would have offered more assurance would have
been a higher up addressing this forum directly. Time will tell.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, July 24, 2002 6:39 PM, Nakama, Mari [SMTP:MNakama-at-feico.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} This message is in response to a Listserver posting concerning Veeco
} and FEI Merger.
} -----------------------------------------------------------
} Since we announced our proposed merger with Veeco Instruments last
} Friday, July 12, some of you have raised concern over our future
} commitment to your needs. FEI has grown as a result of our strong
} technology portfolio and our ability to leverage it across our
} diverse customer base, including the very important scientific
} research sector. The solutions we offer cross over our industry
} sectors more every year. Make no mistake, we intend to continue the
} growth of Veeco FEI through expanded focus on the needs of the
} scientific research industry and microscopists, delivering the tools
} you need to succeed. Please visit our website at www.feicompany.com
} { {http://www.feicompany.com} http://www.feicompany.com} and visit the
} merger information page that is accessible from our home page for
} more on how we intend to serve you better as a result of this merger.
}
} Thank you,
} Vahe' Sarkissian,
} Chairman, president and CEO, FEI Company.
}
}
}
} Cheers,
} George Scholes
} Director of North American Sales
} 7451 NW Evergreen Parkway
} Hillsboro, OR 97124
} PH # 503-640-7644
} FX # 503-640-7663
} gscholes-at-feico.com
}



From daemon Thu Jul 25 03:59:49 2002



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Thu, 25 Jul 2002 10:18:43 +0200 (MET DST)
Subject: Re: Electropolishing

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hi,
Struers sold original solution for electropolishing nickel alloys or ask
this company about composition and technical parameters for tenupol machine,
in the many books from Vander Voort via ASTM handbook to Petzow, or in
the internet you can find data about solution for polishing nickel

best regards

Krzysztof Jan Huebner

{hubner-at-IOd.krakow.pl} :-)

Instytut Odlewnictwa
ul Zakopianska 73 telefon (0-12) 2618111 wew 356
30-418 Krakow faks (0-12) 2660870

On Wed, 24 Jul 2002, Ian MacLaren wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} Any suggestions for the best electropolishing solution and conditions for a
} Nickel-based superalloy (CMSX-4). We have a Struers Tenupol.
}
} Thanks
}
} Ian MacLaren
} N.B. New address
} TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
} Petersenstr. 23, 64287 Darmstadt, Germany
} Tel: +49 6151 162894
} ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/
}
}
}


From daemon Thu Jul 25 04:07:50 2002



From: Sousan Abolhassani :      sousan.abolhassani-at-psi.ch
Date: Thu, 25 Jul 2002 11:01:15 +0200
Subject: Re: Ethical and legal issues in imaging

Contents Retrieved from Microscopy Listserver Archives
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} From a scientifique point of view, microscopy (as a general
term that would define all techniques mentioned in the recent
list messages and other non-mentioned ones), is
a method of OBSERVATION of phenomena (usually) of interest
to the scientifique community.

Therefore, like any other scientifique technique, microscopy
is a tool for the study of phenomena. The data obtained (again
like any other scientifique technique) is to be collected
carefully and subsequently interpreted.

An honest researcher and scientist is expected, when reporting
his/her work, to distinguish between the results of his/her
OBSERVATIONS (i.e. the data) and the INTERPRETATION of those results.

The observations being exposed without prejudice and therefore
without manipulation, should be reported to the scientifique
community, in order that any other scientist could use the
data as a set of facts that can be accumulated for
the understanding of the phenomena, independant from the
way the data is interpreted by the observer.

If so, the observer has the right to give his/her interpretation,
by clearly defining them as such, which if it is done without any
prejudice and by performing a careful analysis, should further help to
elucidate the understanding of the phenomenon, because the
observer is the one who has the closest access to his/her
OBSERVATIONS.

This would mean that any manipulation of data (whether it is images
or other data) would be in fact a self destroying effort in
a scientifique study, the most valuable contribution of the scientist
starting with his/her effort to collect a correct data.

A true scientist in my opinion is one who sincerely wishes to help
advance the science and who does not dogmatically fix his/her mind
to an INTERPRETATION; the history showing us several examples of
the failure of such attitudes, when the discoveries have been
braught by pioneers of modern science.

Regards,

Sousan

Philip Oshel wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} All of these comments raise the issue that there should be a more
} rigorous photography component to any course that teaches microscopy,
} whether as a field of study or as a tool.
} All good microscopy courses teach photography to some extent, but
} this should be expanded, and include more theory. And more material
} normally considered part of the photography courses taught in art
} schools.
} This would have obvious influences on training on the legal and
} ethical issues of imaging in microscopy. Perhaps education should be
} a part of the ethics sessions at M&M.
} Phil
}
} } As much as the example of the model annoyed me, I am going to continue
} } with it to raise some points.
} }
} } Let's say the model had her picture taken by one of her boyfriends, who
} } happened to have a camera with a long telephoto lens. Subsequently the
} } model purchases and uses the new Super-Duper-Triple-D Breast Enhancement
} } device, and diligently follows the instructions for use for three
} } weeks. She then has another picture taken.
} }
} } Scenario 1: The second photo is taken by a different boyfriend, who has
} } his favorite super wide-angle lens on his camera, the one with the
} } pincushion effect that makes objects in the middle of the field appear
} } larger than those to the sides. He has no idea how the first photo was
} } taken.
} }
} } Scenario 2: The second photo is taken by the first boyfriend, who
} } deliberately uses a wide-angle lens this time instead of the telephoto.
} }
} } Scenario 3: In the first two scenarios the model genuinely thinks the
} } device has enlarged her bust.
} }
} } Scenario 4: In the first two scenarios the model knows the device has NOT
} } increased her bust, but knows a bit about photography. She knows that the
} } telephoto lens will make her bust appear smaller due to foreshortening,
} } and the wide angle lens will make it look bigger, and has asked that those
} } particular lenses be used for the photos. The boyfriends are in on this or
} } they are not in on this.
} }
} } Scenario 5: The photo shoots were not set up by an advertising agency for
} } the Super-Duper-Triple-D Breast Enhancement Device.
} }
} } Scenario 6: The photo shoots were set up by an advertising agency for the
} } Super-Duper-Triple-D Breast Enhancement Device.
} }
} } If you look at all the possible combinations and factor in whether each
} } participant knowingly or unknowingly performed their roles, and whether
} } they performed their part either knowingly and maliciously, or
} } unknowingly, or because they were uneducated about the properties of their
} } imaging devices, you can see that the images might or might not represent
} } the "truth". And this is WITHOUT digital or even darkroom
} } manipulation! Throw in differences in lighting and possibly the film type
} } and even the chemicals used to make the prints, and you see that you do
} } not have a controlled experiment here. Add a little personal bias (I might
} } personally consider the model to be an airhead and the first boyfriend to
} } be a fool and the second to be a power-hungry, manipulating character, and
} } so might draw conclusions based on personal bias when viewing the
} } images) and you've got not only flawed data, but a possible
} } misinterpretation of the data.
} }
} } Now let's open these pictures in Photoshop ...
} }
} } Aloha,
} } Tina
} }
} }
} } ****************************************************************************
} } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} } * Biological Electron Microscope Facility * (808) 956-6251 *
} } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} } ****************************************************************************
}
} --
} Philip Oshel
} Supervisor, BBPIC microscopy facility
} Department of Animal Sciences
} University of Wisconsin
} 1675 Observatory Drive
} Madison, WI 53706 - 1284
} voice: (608) 263-4162
} fax: (608) 262-5157 (dept. fax)


From daemon Thu Jul 25 07:04:45 2002



From: Microshaw-at-aol.com
Date: Thu, 25 Jul 2002 07:56:29 -0400
Subject: Poor Angela

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nice scam.

From daemon Thu Jul 25 07:54:19 2002



From: =?ISO-8859-1?Q?G=F6ran_Axelsson?= :      axelsson-at-acc.umu.se
Date: Thu, 25 Jul 2002 14:54:31 +0200
Subject: Re: Software hunt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

OpenOffice was mentioned in a previous posting. When I read a later
answer I added one and one and
realised I should press help in my OpenOffice.
There was one entry for "tiling - pages for printing in presentations"
"Tile pages
Select this option if the pages are to be printed in tile format.
For this purpose, select a page format
that is larger than the paper format."

.. and it won't cost you a cent to try (unless you have a modem
connection :-)

http://www.openoffice.org/

Nuff said!

Regards, Göran Axelsson



swiding wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Jul 25 08:04:45 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Thu, 25 Jul 2002 08:58:06 -0400
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
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Chris,

I've noticed that the list has come up quite consistently saying SEM
doesn't fit the definition. Well technically no in some respects, but
in theory it does use optical properties and principles to create a
probe.

Sorry for draggin this seemingly tiresome discussion on.

For the record - that definition is Dawes - not mine. And When I read
it I'm not sure how it excludes LSM or SEM. But then I'm sure Mel knows
in his mind why. ;)

The end result is still an image that used optics to either create a
probe or a line and taking the information from that line and
translating it into an image.

Forgive me if this bores the list. I'll make every attempt to ignore
the desire to post anything about this subject, from here on out.

Geoff

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Wednesday, July 24, 2002 3:27 PM
To: Geoff Williams
Cc: Microscopy-at-sparc5.microscopy.com


Geoff
Only some light and electron microscopes can be defined as optical in
the sense that the
image is created using lenses.
The scanning electron microscope does not image an object using
lenses.
In the SEM the sole function of the lenses in the electron optical
column
is to create the electron probe with which the specimen is scanned.
Therefore, by your criterion the TEM would be an optical microscope,
but the SEM would not.
In an exactly analogous way, the functions of the lens optics in a
laser scanning confocal microscope
are to form the diffraction limited spot and to spatially filter the
light emitted from that spot. NOT to generate a 2-D image.
Your definition of "optical" would therefore also exclude LSM from
the class Optical Microscopes.
Personally, I don't think either exclusion is particularly useful,
particularly when we consider that many modern
microscopes are hybrids of optical and other technologies. Where does
STEM fit in? Does x-ray microscopy need to
use an x-ray lens to qualify as an optical technique? What is the
status of scanned probe microscopes that use
laser optics to sense probe movement? Should Near-field optical
microscopy be renamed Near-field light microscopy?

Chris

} Optical Microscopy: does that mean it uses a lens system to examine
an
} object? If so then it becomes impossible to separate the Electron
} Microscopes from Light microscopes.
}
} Here are a few terms I use:
} CLM: Conventional Light Microscopy (I learned from Dr. Joanne
Whallon
} and Dr Shirely Owens at Michigan Sate University)
} This has been shortened to just:
} LM: Light microscopy with the assumption made that the light is
between
} UV and IR
}
} And yes - I will get directly to the posed question so as to say on
} topic and focused to Ian MacLaren's original Email:
}
} Light Microscopy works very well and I think deserves the position
of
} being the 'correct' term. LM is a nice simple abbreviation that
goes
} very well with TEM, SEM, AFM, STEM, LSM, CLSM, LSCM (those confocal
} folks need to settle down and pick LSM ;) ).
}
} Make any sense?
}
} Geoff Williams
} Microscopy Facility Supervisor
} Biology Department
} Central Michigan University
} Mt Pleasant, MI 48859
}
}




From daemon Thu Jul 25 08:05:55 2002



From: Margaret M. Miller :      millermm-at-uthscsa.edu
Date: Thu, 25 Jul 2002 07:59:47 -0500
Subject: relocating to Houston,TX

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I know of someone who wishes to relocate to
Houston. If anyone is interested in a hard working,
dedicated team player please send contact information and I will pass it on.

Thanks in advance.



From daemon Thu Jul 25 09:22:51 2002



From: Jay Stanley :      jaystanley-at-cox.net
Date: Thu, 25 Jul 2002 09:20:08 -0500
Subject: TLM :Wild M20 screw

Contents Retrieved from Microscopy Listserver Archives
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Anybody have any idea what the measurment and thread count is on the Wild
M20 condenser set screw (the one that holds the condenser in the carrier)??
Thanks
Jay



From daemon Thu Jul 25 09:22:51 2002



From: kbovard-at-creighton.edu
Date: Thu, 25 Jul 2002 09:14:12 -0500 (CDT)
Subject: Lodging for MSA 2002 in Quebec (fwd)

Contents Retrieved from Microscopy Listserver Archives
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I just found out that I have been OK'd to go to the MSA 2002 Conference in
Quebec City. Now I'm scrambling to find a hotel. All of the hotels that
the conference cites are full and from what I can tell about the city,
many other hotels are full too. Does anyone have any leads as to a
reasonably-priced hotel that is close to the Convention Center. I am a
woman traveling alone and so safety is a high concern. I really don't
need a 4-star hotel either. Additionally, does anyone need a roommate. I
am a non-smoker, non-partier and consider myself a fairly respectful
roommate. It is nice to have someone to watch out for you in a strange
place.

Sincerely,
Karen Bovard
Creighton University Medical Center
Pathology Electron Microscopy Lab
Omaha, Nebraska

(402-280-4651)




From daemon Thu Jul 25 09:44:01 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 25 Jul 2002 09:37:22 -0500
Subject: Re: Imaging Micro-organisms

Contents Retrieved from Microscopy Listserver Archives
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Peter,

Welcome to the wonderful world of taxonomy and systematics. There is
no simple answer to your question, other than "It depends, what kind
of critter are we talking about?"
Very little can be said about bacteria on the basis of simple
morphology. Gross descriptions can be made, as in "rod-shaped",
"coccus", "coccoid in a tetrad", and the like, but that's about it.
Much more information not obtainable from morphology alone is needed.
Other microbial organisms must be identified by morphology as seen in
the light microscope. Ciliates for example can often be gotten to
family in the SEM, sometimes to genus (I wouldn't trust species), and
light microscopy is required for species identification -- this is
usually done by silver staining of the kinetochores at the bases of
the cilia.
Some of the testate taxa of amoebae can be ID'd to lower levels by
the form of the test. Here, I mean the forms that construct little
houses to live in, *not* the forms that secrete "shells". or
"skeletons" (I'm avoiding the correct technical terms here to get the
idea across).
Foraminiferans, Radiolarians, Acantharia (with strontium sulphate
"skeletons" no less) and other such amoeboid forms, and groups such
as diatoms, and other "skeleton" forming groups are ID'd to species
by light microscopic morphology or SEM, after removal of the organic
bits. Either by killing and chemical means, or a few thousands or
millions of years of geological time.
Diatoms also used to be ID'd by producing metal-shadowed carbon
replicas of the test and imaging them in the TEM.
Groups that grow plates on the outside, such as some dinoflagellates
and coccolithophorids are ID'd often or exclusively on the morphology
of the plates, and this requires light microscopy or SEM.

Generally speaking, Protistans are all identified by light
microscopy. Some IDs may now be made by SEM or even TEM, but usually
these are supplementary methods for studies of evolutionary
relationships.
This is also true for most groups -- up to the phylum level -- of
small metazoans and algae, and indeed for macroscopic critters,
microscopic data may be either required or useful for identification.
Bacteria (in the broad sense), no. They can be grouped into broad
categories, but without extensive information on colony formation,
metabolic requirements and products, behavior (such as motility) and
so forth, they can't be ID'd by morphology.
Many of us have been doing "electronic bio-identification" for
decades. The future is there, if only we can convince the people that
sign the paychecks. Until then, we'll run microscopy facilities.
A good place to start is Dave Scharf's SEM books for neat images with
good information, and a good invertebrate text, such as Brusca and
Brusca "Invertebrate Zoology". But there are a wealth of such
sources, and a bookstore with a good nature section will have some on
their shelves. Fun browsing.

Phil

} Folks;
}
} Since many of you do biological imaging vis-a-vis SEM and "optical" means, I
} have a question. When imaging bacteria or any micro-organism, is the species
} identified by it's shape, general apperance, size etc.? Or better yet, just
} how is it identified in the EM? Or does this require some other assay for
} identification and then it is imaged to generate other information? I don't
} image bio materials but it is something that I would like to be able to show
} my daughter who is interested in the micro-biological world. I see a future
} in electronic bio-identification and since her interests seem to lie in that
} direction, I'd like to explain some of this intelligently.
}
} Peter

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Thu Jul 25 12:28:20 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 25 Jul 2002 11:28:41 -0600
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Geoff,

I think you said it yourself: In one case optics is used to create a probe
(not an image), in the other case optics is used to create the image itself.


Would you call an EDS spectrum also an "optical image"? It is created using
the same probe you used for the micrograph (or "SEM image").

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Geoff Williams [mailto:willi1gl-at-cmich.edu]
Sent: Thursday, July 25, 2002 6:58 AM
To: 'Chris Jeffree'
Cc: Microscopy-at-sparc5.microscopy.com


Chris,

I've noticed that the list has come up quite consistently saying SEM
doesn't fit the definition. Well technically no in some respects, but
in theory it does use optical properties and principles to create a
probe.

Sorry for draggin this seemingly tiresome discussion on.

For the record - that definition is Dawes - not mine. And When I read
it I'm not sure how it excludes LSM or SEM. But then I'm sure Mel knows
in his mind why. ;)

The end result is still an image that used optics to either create a
probe or a line and taking the information from that line and
translating it into an image.

Forgive me if this bores the list. I'll make every attempt to ignore
the desire to post anything about this subject, from here on out.

Geoff

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Wednesday, July 24, 2002 3:27 PM
To: Geoff Williams
Cc: Microscopy-at-sparc5.microscopy.com


Geoff
Only some light and electron microscopes can be defined as optical in
the sense that the
image is created using lenses.
The scanning electron microscope does not image an object using
lenses.
In the SEM the sole function of the lenses in the electron optical
column
is to create the electron probe with which the specimen is scanned.
Therefore, by your criterion the TEM would be an optical microscope,
but the SEM would not.
In an exactly analogous way, the functions of the lens optics in a
laser scanning confocal microscope
are to form the diffraction limited spot and to spatially filter the
light emitted from that spot. NOT to generate a 2-D image.
Your definition of "optical" would therefore also exclude LSM from
the class Optical Microscopes.
Personally, I don't think either exclusion is particularly useful,
particularly when we consider that many modern
microscopes are hybrids of optical and other technologies. Where does
STEM fit in? Does x-ray microscopy need to
use an x-ray lens to qualify as an optical technique? What is the
status of scanned probe microscopes that use
laser optics to sense probe movement? Should Near-field optical
microscopy be renamed Near-field light microscopy?

Chris

} Optical Microscopy: does that mean it uses a lens system to examine
an
} object? If so then it becomes impossible to separate the Electron
} Microscopes from Light microscopes.
}
} Here are a few terms I use:
} CLM: Conventional Light Microscopy (I learned from Dr. Joanne
Whallon
} and Dr Shirely Owens at Michigan Sate University)
} This has been shortened to just:
} LM: Light microscopy with the assumption made that the light is
between
} UV and IR
}
} And yes - I will get directly to the posed question so as to say on
} topic and focused to Ian MacLaren's original Email:
}
} Light Microscopy works very well and I think deserves the position
of
} being the 'correct' term. LM is a nice simple abbreviation that
goes
} very well with TEM, SEM, AFM, STEM, LSM, CLSM, LSCM (those confocal
} folks need to settle down and pick LSM ;) ).
}
} Make any sense?
}
} Geoff Williams
} Microscopy Facility Supervisor
} Biology Department
} Central Michigan University
} Mt Pleasant, MI 48859
}
}




From daemon Thu Jul 25 12:48:37 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 25 Jul 2002 13:46:12 -0700
Subject: fiber optic coupling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are purchasing a small UV laser (Oriel 79111 -- any comment son this
welcome too) for zapping cells in an OPTICAL MICROSCOPE at 337nm.

Instead of dealing with mirros and an open beam in the lab, Oriel
recommends its fiber optic which has a standard SMA termination.

We're new at this laser stuff. Does anybody know how to connect the fiber
to the UV in port at the back of an Olympus microscope?

Thanks!


____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/



From daemon Thu Jul 25 16:13:32 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 25 Jul 2002 16:05:06 -0500
Subject: Blade holder for high profile, heavy duty blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

We are looking for a new blade holder that will work in a sliding
microtome to section celloidin embedded temporal bones. We want
to use high profile blades that are heavy duty to reduce chatter.
We found several sources for the blades, but we can't find a holder
that will hold a blade that is both high profile AND heavy duty
(.033"-.035" thick).Does anyone know of a supplier of a holder for
this type of blade?

Thanks in advance,
Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas



From daemon Thu Jul 25 17:08:42 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 25 Jul 2002 17:02:03 -0500
Subject: Re: Lodging for MSA 2002 in Quebec (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't seem to have the URL anymore, but go to the Quebec City web
site (I got it by a Google search) and go to "accomodations". Click
on the downtown and old city areas, and you'll get about 100 hotels,
most of them cheaper than the meeting hotels, and within 5 - 10
minutes walk of the convention center.
Phil

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Thu Jul 25 18:47:12 2002



From: Benyam Estifanos :      benyam-at-mac.com
Date: Thu, 25 Jul 2002 19:32:38 -0400
Subject: Re: Lodging for MSA 2002 in Quebec (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,

You may try the following web site:
http://www.bbselect.com
or call them 1 800 741-1617 (Reservation-at-BBSelect.com).

Regards,
Benyam

On Thursday, July 25, 2002, at 10:14 AM,
"kbovard-at-creighton.edu"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I just found out that I have been OK'd to go to the MSA 2002 Conference
} in
} Quebec City. Now I'm scrambling to find a hotel. All of the hotels
} that
} the conference cites are full and from what I can tell about the city,
} many other hotels are full too. Does anyone have any leads as to a
} reasonably-priced hotel that is close to the Convention Center. I am a
} woman traveling alone and so safety is a high concern. I really don't
} need a 4-star hotel either. Additionally, does anyone need a
} roommate. I
} am a non-smoker, non-partier and consider myself a fairly respectful
} roommate. It is nice to have someone to watch out for you in a strange
} place.
}
} Sincerely,
} Karen Bovard
} Creighton University Medical Center
} Pathology Electron Microscopy Lab
} Omaha, Nebraska
}
} (402-280-4651)
}
}
}



From daemon Thu Jul 25 19:14:32 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 25 Jul 2002 21:28:12 -0400
Subject: fiber optic coupling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you tried TBS?

-----Original Message-----
} From: Karen Pawlowski [mailto:kpawlow-at-swbell.net]
Sent: July 25, 2002 3:05 PM
To: Histology Net List Server (E-Mail); MSA listserver submission


Michael;

I have a New Wave Corp. laser I use on a micromanipulator in the lab.
[semiconductors]. It is used to cut thin and thick films of metal in the
range of 400 angstroms to several microns. However, this laser was married
to a Mitutoyo widefield microscope and a filter was placed in the optical
path in front of the eyepieces for safety reasons by New Wave. I strongly
recommend that you check with the mfg. and your safety officer, if you have
one, or OSHA, relative to the possibility of eye injury. You don't want to
find out later that you are generating lesions in your retina when firing
it. The calculation for permissible emission is somewhat complex and
involves wavelength, power, pulse width and repetition rate so it's not
particularly straightforward. I assume this is a pulsed laser application.
If CW [continuos wave], the allowable energy changes.

Peter Tomic
Anadigics, Inc.
Warren, New Jersey

-----Original Message-----
} From: Michael Cammer [mailto:cammer-at-aecom.yu.edu]
Sent: Thursday, July 25, 2002 4:46 PM
To: microscopy-at-sparc5.microscopy.com


We are purchasing a small UV laser (Oriel 79111 -- any comment son this
welcome too) for zapping cells in an OPTICAL MICROSCOPE at 337nm.

Instead of dealing with mirros and an open beam in the lab, Oriel
recommends its fiber optic which has a standard SMA termination.

We're new at this laser stuff. Does anybody know how to connect the fiber
to the UV in port at the back of an Olympus microscope?

Thanks!


____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.

Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461

(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/



From daemon Thu Jul 25 20:35:36 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 25 Jul 2002 20:29:47 -0500
Subject: Re: Blade holder for high profile, heavy duty blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Robin,

I did talk to TBS and they said none of their holders accomodate the
highprofile-heavy duty blades. The only holder they have that
accomodates heavy duty blades is a low profile holder.

Karen

Robin Thain wrote:
}
} Have you tried TBS?
}
} -----Original Message-----
} From: Karen Pawlowski [mailto:kpawlow-at-swbell.net]
} Sent: July 25, 2002 3:05 PM
} To: Histology Net List Server (E-Mail); MSA listserver submission
} Subject: Blade holder for high profile, heavy duty blades
}
} Hi everyone,
}
} We are looking for a new blade holder that will work in a sliding
} microtome to section celloidin embedded temporal bones. We want
} to use high profile blades that are heavy duty to reduce chatter.
} We found several sources for the blades, but we can't find a holder
} that will hold a blade that is both high profile AND heavy duty
} (.033"-.035" thick).Does anyone know of a supplier of a holder for
} this type of blade?
}
} Thanks in advance,
} Karen Pawlowski, Ph.D.
} Research Scientist
} UT Dallas



From daemon Fri Jul 26 05:16:25 2002



From: heckman-at-bgnet.bgsu.edu
Date: Fri, 26 Jul 2002 06:06:18 -0500
Subject: dye specific for collagen or fibrin?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear list-ers-
I got an inquiry from a former student:
"I'm looking for a dye/stain
that we could use on native collagen or elastin or fibrin as an
indicator and that would then give us a single peak absorbance at about
650 nm or higher. I've found numerous dyes, but they do not have the
single peak absorbance many times or else they are mostly for use with
nucleotide labeling, which isn't what we're after here. Many dyes I've
found require use with some sort of denaturing agent that would change
the structure of the protein, something we want to avoid. Can you help
by either suggesting some dyes we may want to take a look at or some
companies that we may want to look to for something of this nature?"
Many thanks,
Carol Heckman
Bowling Green State University




From daemon Fri Jul 26 07:39:16 2002



From: joe.p.neilly-at-abbott.com
Date: Fri, 26 Jul 2002 07:30:31 -0500
Subject: Re: Lodging for MSA 2002 in Quebec (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I found the following lists very helpful. Many hotels are available in the
surrounding areas.

http://www.highwayhome.com/travel/tourismbycity/quebeccity/quebeccityhotels.html

http://www.quebecregion.com/e/hebergement.asp

Joe Neilly, Research Investigator
Abbott Laboratories
R45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Fri Jul 26 07:45:34 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Fri, 26 Jul 2002 08:39:42 -0400
Subject: Re: Terminology: optical microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike, (and all)

I wouldn't call it an "optical image" BUT it does use 'optics' to create
the probe. And the optics are a critical part of the system. And thus
optical microscopy.

But if you break down what the spectrum really is, it is a
representation of the spectrum, is it not? And it is a visual
representation in as much as it is a graphical display of the number of
counts at each energy point, correct? So then you could call the EDS
spectrum an optical image, esp since the end result is presented for a
final detector commonly used in conventional light microscopy, which is
the Human Retina. Now of course the visual display of the spectrum is
really just a bunch of numbers, and depending on the output and the
desired information in that bunch of numbers the spectrum display is
rather unimportant at times. In as much though, as you combine the
pieces and the collection of instrumentation to examine it, you could
call it an optical image. But I know there are too many folks out there
that are having a hard time following, or understanding my perspective
on this. I suppose the reason why I find it so important is that it is
a fundamental and critical cohesive link or binding agent that
demonstrates the interrelationship of the different ways we examine
things. Kinda like the similarity that all 'Trees' have. Sure they are
different and do different things but they all can be put together in a
common way.

I was thinking about it during my commute. GO back to the basic, the
archetypal form, of the microscope, then follow the derivatives, stop
looking at just the piece of the system that is important to YOU and
look at it as a whole. Does not nearly every type of microscope use
optics? And thus does not the term optical microscope seem rather
redundant?

It would be like calling the absence of color (meaning black), dark
black. Black is dark. No shades for the absence of color. But then I
suppose I just pulled in a whole separate issue of another razor - what
is color and how to some people define it compared to others.

Respectfully to all, esp to those that find this a waste of bandwidth,
Geoff Williams

-----Original Message-----
} From: Mike Bode [mailto:mb-at-Soft-Imaging.com]
Sent: Thursday, July 25, 2002 1:29 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Geoff,

I think you said it yourself: In one case optics is used to create a
probe
(not an image), in the other case optics is used to create the image
itself.


Would you call an EDS spectrum also an "optical image"? It is created
using
the same probe you used for the micrograph (or "SEM image").

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Geoff Williams [mailto:willi1gl-at-cmich.edu]
Sent: Thursday, July 25, 2002 6:58 AM
To: 'Chris Jeffree'
Cc: Microscopy-at-sparc5.microscopy.com


Chris,

I've noticed that the list has come up quite consistently saying SEM
doesn't fit the definition. Well technically no in some respects, but
in theory it does use optical properties and principles to create a
probe.

Sorry for draggin this seemingly tiresome discussion on.

For the record - that definition is Dawes - not mine. And When I read
it I'm not sure how it excludes LSM or SEM. But then I'm sure Mel knows
in his mind why. ;)

The end result is still an image that used optics to either create a
probe or a line and taking the information from that line and
translating it into an image.

Forgive me if this bores the list. I'll make every attempt to ignore
the desire to post anything about this subject, from here on out.

Geoff

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Wednesday, July 24, 2002 3:27 PM
To: Geoff Williams
Cc: Microscopy-at-sparc5.microscopy.com


Geoff
Only some light and electron microscopes can be defined as optical in
the sense that the
image is created using lenses.
The scanning electron microscope does not image an object using
lenses.
In the SEM the sole function of the lenses in the electron optical
column
is to create the electron probe with which the specimen is scanned.
Therefore, by your criterion the TEM would be an optical microscope,
but the SEM would not.
In an exactly analogous way, the functions of the lens optics in a
laser scanning confocal microscope
are to form the diffraction limited spot and to spatially filter the
light emitted from that spot. NOT to generate a 2-D image.
Your definition of "optical" would therefore also exclude LSM from
the class Optical Microscopes.
Personally, I don't think either exclusion is particularly useful,
particularly when we consider that many modern
microscopes are hybrids of optical and other technologies. Where does
STEM fit in? Does x-ray microscopy need to
use an x-ray lens to qualify as an optical technique? What is the
status of scanned probe microscopes that use
laser optics to sense probe movement? Should Near-field optical
microscopy be renamed Near-field light microscopy?

Chris

} Optical Microscopy: does that mean it uses a lens system to examine
an
} object? If so then it becomes impossible to separate the Electron
} Microscopes from Light microscopes.
}
} Here are a few terms I use:
} CLM: Conventional Light Microscopy (I learned from Dr. Joanne
Whallon
} and Dr Shirely Owens at Michigan Sate University)
} This has been shortened to just:
} LM: Light microscopy with the assumption made that the light is
between
} UV and IR
}
} And yes - I will get directly to the posed question so as to say on
} topic and focused to Ian MacLaren's original Email:
}
} Light Microscopy works very well and I think deserves the position
of
} being the 'correct' term. LM is a nice simple abbreviation that
goes
} very well with TEM, SEM, AFM, STEM, LSM, CLSM, LSCM (those confocal
} folks need to settle down and pick LSM ;) ).
}
} Make any sense?
}
} Geoff Williams
} Microscopy Facility Supervisor
} Biology Department
} Central Michigan University
} Mt Pleasant, MI 48859
}
}





From daemon Fri Jul 26 07:54:52 2002



From: mark.lewis-at-thermo.com
Date: Fri, 26 Jul 2002 08:48:53 -0400
Subject: Re: Blade holder for high profile, heavy duty blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Karen,

Thermo Shandon sells a disposable holder that holds heavy duty disposable
blades. I am not sure if it will fit your microtome or not, but it is 130
mm long and 39 mm in height. The order number is 47.

Call me if I can be of further assistance.

Best regards,

Mark

Mark Lewis
Product Specialist
Thermo Shandon
171 Industry Drive, Pittsburgh, PA 15275 USA
Direct: (412) 747-4013
Fax: (412) 788-1097
E-mail: mark.lewis-at-thermoshandon.com

This e-mail message and all attachments transmitted herewith are trade
secret and/or confidential information intended only for the viewing and
use of the addressee.  If the reader of this message is not the intended
recipient, you are hereby notified that any review, use, communication,
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prohibited.  If you have received this communication in error, please
notify the sender immediately by telephone, or electronic mail, and delete
this message and all copies and backups thereof.  Thank you for your
cooperation.






Karen
Pawlowski To: Robin Thain {rthain-at-telusplanet.net}
{kpawlow-at-swbe cc: "Histology Net List Server (E-Mail)"
ll.net} {histonet-at-pathology.swmed.edu} , MSA listserver
submission {Microscopy-at-sparc5.microscopy.com}
07/25/2002 Subject: Re: Blade holder for high profile,
09:29 PM heavy duty blades






Hi Robin,

I did talk to TBS and they said none of their holders accomodate the
highprofile-heavy duty blades. The only holder they have that
accomodates heavy duty blades is a low profile holder.

Karen

Robin Thain wrote:
}
} Have you tried TBS?
}
} -----Original Message-----
} From: Karen Pawlowski [mailto:kpawlow-at-swbell.net]
} Sent: July 25, 2002 3:05 PM
} To: Histology Net List Server (E-Mail); MSA listserver submission
} Subject: Blade holder for high profile, heavy duty blades
}
} Hi everyone,
}
} We are looking for a new blade holder that will work in a sliding
} microtome to section celloidin embedded temporal bones. We want
} to use high profile blades that are heavy duty to reduce chatter.
} We found several sources for the blades, but we can't find a holder
} that will hold a blade that is both high profile AND heavy duty
} (.033"-.035" thick).Does anyone know of a supplier of a holder for
} this type of blade?
}
} Thanks in advance,
} Karen Pawlowski, Ph.D.
} Research Scientist
} UT Dallas








From daemon Fri Jul 26 08:53:15 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 26 Jul 2002 09:44:20 -0400
Subject: FW: soil aggregates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I forward this thread to the Microscopy list in the hope of getting more of
a response for these folks that I was able to provide. If that is true, I
think they will appreciate the help. I like the part about sectioning dust
in sulfur (cake I suppose) and realized that most of us, who have delved
into the mysteries of infiltrating and embedding, have spent some time
sectioning 'dust' embedded in paraffin or plastic of one sort or another
whether we knew it at the time or not. Knowing that dust is in and on
everything we work with, breath and eat makes one consider the advisability
of moving to a bubble combo of workshop and home. Well, not immediately.

The needy are: MaryLou: mim11-at-cornell.edu and Bob Schoonhoven:
rschoon-at-email.unc.edu

Hope there's some real dirty expertise out there. It looks like we're in
the loop for a real ESEM here, and I better start learning about what to
expect from dirt and soil in my microscope.

Thanks and for those going, have fun in Quebec.

Fred Monson

} ----------
} From: MaryLou
} Sent: Friday, July 26, 2002 7:08 AM
} To: Monson, Frederick C.
} Cc: histonet-at-pathology.swmed.edu
} Subject: RE: soil aggregates
}
}
} Good Morning Fred,
}
} Your internet searching capabilities are astounding. I thank you extremely
}
} much for all the sites you sent. This should keep the dirt people happy
} for a long time. While I enjoy challenges, this one was just a tad out of
}
} my realm. The critter eyes are piling up and I must get back to the
} safety
} of paraffin :)
}
} Thanks again,
} Mary Lou
}
}
}
} At 02:35 PM 7/25/2002 -0400, you wrote:
} } Mary Lou,
} }
} } You sound like one who has been working this area, but you are
} } asking a question that I would consider elementary for a material complex
} } such as soil. Thus, I did my usual and instituted a search to see what I
} } could find, because I am likely to see similar questions within the near
} } future.
} }
} } For me, a real beginner, I think I will spend time here, and then start
} } conversing with the experts whose methods are cited.
} }
} } http://www.soils.org/divs/s9/micromorph/micro.html
} }
} } From what I see, most of what workers in dirt consider "thin sectioning"
} } involves grinding and/or sawing. When I first had to work with a truly
} hard
} } substance, I immediately found myself in the domain of the material
} } scientist. Geologists don't ordinarily consider cutting a thin section
} as
} } we do, they think of grinding one - just like the ground bone sections
} that
} } one finds in almost all elementary histology slide sets.
} }
} } Aggregates of micro-size particles can be mounted and ground, if they are
} on
} } the macro- side of micro-. If smaller, and it is paramount that the
} } aggregates NOT be disturbed, then I would turn, as rapidly as possible,
} to
} } more esoteric methods such as ion or plasma etching which can be used on
} } embedded material and can, apparently be very productive.
} }
} } Here are a couple sites that might help to present the degree to which
} } technology using electron optics and focused ion beams or plasmas are
} used
} } in both analysis and production.
} }
} } http://www.mrsec.harvard.edu/facilities.html
} }
} } http://www.mmc.or.jp/std/5.htm [this is a gas!]
} }
} } http://www.feicompany.com/eng/data/trimming.html
} }
} } Finally, Ion Beam Milling at,
} }
} } http://www.ionbeammilling.com/IONBEAMMILLINGILLUSTRETION.htm
} }
} }
} } Hope this helps,
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging
} } Schmucker II Science Center
} } West Chester University
} } South Church Street and Rosedale
} } West Chester, Pennsylvania, USA, 19383
} } Phone: 610-738-0437
} } FAX: 610-738-0437
} } fmonson-at-wcupa.edu
} } CASI URL: http://darwin.wcupa.edu/casi/
} } WCUPA URL: http://www.wcupa.edu/
} } Visitors URL: http://www.wcupa.edu/_visitors/
} }
} } } ----------
} } } From: MaryLou
} } } Sent: Wednesday, July 24, 2002 12:31 PM
} } } To: histonet-at-pathology.swmed.edu
} } } Cc: dawit Solomon
} } } Subject: soil aggregates
} } }
} } } Dear Histonetters,
} } }
} } } A colleague is wanting to see inside soil aggregates of varying
} } } thicknesses, up to several hundred microns. I was able to make
} paraffin
} } } sections, 20 microns, by soaking the samples in paraffin for many
} } } hours. No solvents allowed. A researcher at NASA gets 1 micron
} sections
} } } from his dust particles in sulfur. We have no idea how he does it.
} } } Thinner is better. Any suggestions out there? Do any bone grinders
} have
} } } any
} } } ideas? Do you know of anybody else we can ask?
} } } Please include Dawit in your responses. Dawit, do you have anything to
} } } add?
} } }
} } } Thank you very much.
} } } Mary Lou
} } }
} } }
} } }
}
}


From daemon Fri Jul 26 10:10:23 2002



From: Mike McKay :      mike.mckay-at-vitana.com
Date: Fri, 26 Jul 2002 11:01:13 -0400
Subject: Manipulation of images.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In reply to Sousan Abolhassani who wrote
"This would mean that any manipulation of data (whether it is images
or other data) would be in fact a self destroying effort in
a scientifique study, the most valuable contribution of the scientist
starting with his/her effort to collect a correct data."

My meager understanding of the scientific method is that, in reporting the
interpretation of data, the scientist has to completely describe the manner
and methods by which the data was captured and the interpretation drawn.
The requirement is that a subsequent scientists should be able to reproduce
the conditions of the experiment and recreate the data. Or, using the raw
data, recreate the manipulated image.

In this context, scientific images do not preclude manipulation and analysis
as long as the algorithms used are described in such a way that their effect
on the data is clear. Add to the algorithms the experimental setup used to
capture the images and the complete "picture" allows peers to assess and
test the validity of the interpretation.

Yes, the scientist may want to keep the raw image data for future use or to
share with others. But in reporting results (the interpretation), the raw
data is often too cumbersome to be useful. Manipulation, then, is a very
useful tool to make sense of difficult and cumbersome data including images.

Yours,

Michael McKay

{ { { { { { { { { { { { { { { { { { { { { { { P i x e L I N K } } } } } } } } } } } } } } } } } } } } } } } } }
Michael McKay, Product Manager {mailto:mike.mckay-at-vitana.com}
Vitana Corporation
2500 Don Reid Drive Tel: (613) 247-1211 x 152
Ottawa, Ontario Cell: (613) 859-6174
Canada K1H 1E1 Fax: (613) 247-2001
"Making Digital Imaging Simple {http://www.pixelink.com} "
{ { { { { { { { { { { { { { { { { { { { { { { P i x e L I N K } } } } } } } } } } } } } } } } } } } } } } } } }




From daemon Fri Jul 26 14:55:40 2002



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Fri, 26 Jul 2002 14:47:57 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please unsubscribe for now.
thanks




From daemon Fri Jul 26 16:32:46 2002



From: Louise_Harner-at-albint.com
Date: Fri, 26 Jul 2002 17:27:28 -0400
Subject: Re: FW: soil aggregates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking at the comment about a NASA researcher obtaining 1 micron sections
from "dust particles in sulfur", my first thought is that the researcher
might be mounting the dust in polymeric sulfur and then sectioning it.
While I've never heard of this before, I imagine it would work.

The crystal form of sulfur depends on temperature. I don't have my CRC
handbook here to check for temperature/form/stability info. However a quick
check at:
http://www.starcrete.com/info.htm
gives a melting point of 120 °C for their current version of sulfur
concrete (a polymeric sulfur based chemical-resistant substitute for
Portland cement concrete). They use an additive to stabilize the sulfur in
the orthorhombic form over a wide temperature range. Since the polymeric
sulfur bonds to aggregate (i.e., rocks, gravel, sand, etc.) to create the
sulfur concrete, I would assume it would infiltrate and bond with the soil
fairly well. I don't know how easily it can be sectioned with
biological-type equipment; as noted, a lot of us on the materials side use
lapidary equipment rather than microtomes. You could contact STARcrete(TM)
Technologies, Inc. (I have no relationship with them) and ask if they have
a microscopist on staff who could help you (in the links section of their
website, there is an ASTM paper mentioned with a comment about SEM showing
the microstructure). Alternatively, someone at the International Cement
Microscopy Association might have experience with this. Of course, you
could always contact the researcher at NASA, but that would take the fun
out of it for the rest of us.

- Louise

Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com





"Monson,
Frederick C." To: "'List-Microscopy'" {Microscopy-at-sparc5.microscopy.com}
{fmonson-at-wcupa.ed cc:
u} Subject: FW: soil aggregates

2002/07/26 09:44
AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I forward this thread to the Microscopy list in the hope of getting more of
a response for these folks that I was able to provide. If that is true, I
think they will appreciate the help. I like the part about sectioning dust
in sulfur (cake I suppose) and realized that most of us, who have delved
into the mysteries of infiltrating and embedding, have spent some time
sectioning 'dust' embedded in paraffin or plastic of one sort or another
whether we knew it at the time or not. Knowing that dust is in and on
everything we work with, breath and eat makes one consider the advisability
of moving to a bubble combo of workshop and home. Well, not immediately.

The needy are: MaryLou: mim11-at-cornell.edu and Bob Schoonhoven:
rschoon-at-email.unc.edu

Hope there's some real dirty expertise out there. It looks like we're in
the loop for a real ESEM here, and I better start learning about what to
expect from dirt and soil in my microscope.

Thanks and for those going, have fun in Quebec.

Fred Monson

} ----------
} From: MaryLou
} Sent: Friday, July 26, 2002 7:08 AM
} To: Monson, Frederick C.
} Cc: histonet-at-pathology.swmed.edu
} Subject: RE: soil aggregates
}
}
} Good Morning Fred,
}
} Your internet searching capabilities are astounding. I thank you
extremely
}
} much for all the sites you sent. This should keep the dirt people happy
} for a long time. While I enjoy challenges, this one was just a tad out
of
}
} my realm. The critter eyes are piling up and I must get back to the
} safety
} of paraffin :)
}
} Thanks again,
} Mary Lou
}
}
}
} At 02:35 PM 7/25/2002 -0400, you wrote:
} } Mary Lou,
} }
} } You sound like one who has been working this area, but you are
} } asking a question that I would consider elementary for a material
complex
} } such as soil. Thus, I did my usual and instituted a search to see what
I
} } could find, because I am likely to see similar questions within the near
} } future.
} }
} } For me, a real beginner, I think I will spend time here, and then start
} } conversing with the experts whose methods are cited.
} }
} } http://www.soils.org/divs/s9/micromorph/micro.html
} }
} } From what I see, most of what workers in dirt consider "thin
sectioning"
} } involves grinding and/or sawing. When I first had to work with a truly
} hard
} } substance, I immediately found myself in the domain of the material
} } scientist. Geologists don't ordinarily consider cutting a thin section
} as
} } we do, they think of grinding one - just like the ground bone sections
} that
} } one finds in almost all elementary histology slide sets.
} }
} } Aggregates of micro-size particles can be mounted and ground, if they
are
} on
} } the macro- side of micro-. If smaller, and it is paramount that the
} } aggregates NOT be disturbed, then I would turn, as rapidly as possible,
} to
} } more esoteric methods such as ion or plasma etching which can be used on
} } embedded material and can, apparently be very productive.
} }
} } Here are a couple sites that might help to present the degree to which
} } technology using electron optics and focused ion beams or plasmas are
} used
} } in both analysis and production.
} }
} } http://www.mrsec.harvard.edu/facilities.html
} }
} } http://www.mmc.or.jp/std/5.htm [this is a gas!]
} }
} } http://www.feicompany.com/eng/data/trimming.html
} }
} } Finally, Ion Beam Milling at,
} }
} } http://www.ionbeammilling.com/IONBEAMMILLINGILLUSTRETION.htm
} }
} }
} } Hope this helps,
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging
} } Schmucker II Science Center
} } West Chester University
} } South Church Street and Rosedale
} } West Chester, Pennsylvania, USA, 19383
} } Phone: 610-738-0437
} } FAX: 610-738-0437
} } fmonson-at-wcupa.edu
} } CASI URL: http://darwin.wcupa.edu/casi/
} } WCUPA URL: http://www.wcupa.edu/
} } Visitors URL: http://www.wcupa.edu/_visitors/
} }
} } } ----------
} } } From: MaryLou
} } } Sent: Wednesday, July 24, 2002 12:31 PM
} } } To: histonet-at-pathology.swmed.edu
} } } Cc: dawit Solomon
} } } Subject: soil aggregates
} } }
} } } Dear Histonetters,
} } }
} } } A colleague is wanting to see inside soil aggregates of varying
} } } thicknesses, up to several hundred microns. I was able to make
} paraffin
} } } sections, 20 microns, by soaking the samples in paraffin for many
} } } hours. No solvents allowed. A researcher at NASA gets 1 micron
} sections
} } } from his dust particles in sulfur. We have no idea how he does it.
} } } Thinner is better. Any suggestions out there? Do any bone grinders
} have
} } } any
} } } ideas? Do you know of anybody else we can ask?
} } } Please include Dawit in your responses. Dawit, do you have anything
to
} } } add?
} } }
} } } Thank you very much.
} } } Mary Lou
} } }
} } }
} } }
}
}







From daemon Fri Jul 26 17:01:27 2002



From: ncontisfsu-at-netscape.net (Nelson Conti)
Date: Fri, 26 Jul 2002 17:55:16 -0400
Subject: RE: Imaging Micro-organisms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Peter Tomic {PTomic-at-anadigics.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Folks;
} }
} } Since many of you do biological imaging vis-a-vis SEM and "optical" means, I
} } have a question. When imaging bacteria or any micro-organism, is the species
} } identified by it's shape, general apperance, size etc.? Or better yet, just
} } how is it identified in the EM? Or does this require some other assay for
} } identification and then it is imaged to generate other information? I don't
} } image bio materials but it is something that I would like to be able to show
} } my daughter who is interested in the micro-biological world. I see a future
} } in electronic bio-identification and since her interests seem to lie in that
} } direction, I'd like to explain some of this intelligently.
} }
} } Peter
} }
} }
} }
} }
} Dear Peter-
} Although I haven't tried to identify micro-organisms (such as bacteria) by way of SEM, my background (at both Bachelor's and Master's) is in microbiology, and I have taken a class on electron microscopy.
} So, here's my opinion:
} I believe that, in general, the identification of bacteria is done by evaluating a number of characteristics such as shape, general appearance, movement and (sometimes) specialized tests such as blood agar plates for Streptococcus aureus (the bug that can cause strep throat). Thus, I would imagine that the purpose of using SEM lies in obtaining more information about that particular bacterium other than what information you can get by light microscopy. That is, SEM allows the user to identify, say, a particular surface protein, and so on; conversely, light microscopy is ideal to identify that bacterium's particular shape (rod, coccus, etc), motility (or lack of), and so on. As to its identification by EM .. I'm personally unaware of anyone trying to determine a particular bacterium by that type of microscopy because it's been my experience, at least at the intro level in college, for students to use light microscopes, make wet mounts, etc. for basic identification of bacteri!
a.
} A few comments:
} Personally, I'm pleased that your daughter has decided to be keen on learning more about micro-organisms because they are decidedly a very interesting group from a historical standpoint (diseases) and for the very important role they play in the environment.
} My Master's thesis dealt with eukaryotic micro-organisms called ciliates which are part of a larger ensemble named protozoa. In your time in high school, you may recall seeing a long-ish organism with "hair" (err, cilia) all over its surface with many genera-- it would be Paramecium sp.
} I mention the above because ciliates can be thought of as micro-organisms much like our "standard" examples--bacteria or yeast. I found that EM is especially helpful for identifying details of some part of a ciliate such as its mouth (a place where food is taken in for nourishment and energy for cell division).
} Nelson Conti
}


--
164 Ferne Court
Palo Alto, CA 94306
Email: [ncontiSFSU-at-netscape.net]



__________________________________________________________________
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From daemon Sat Jul 27 17:11:28 2002



From: Tom Malis :      malis-at-nrcan.gc.ca
Date: Sat, 27 Jul 2002 17:55:42 -0400
Subject: Re: soil aggregates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If the aggregate holds together reasonably well and don't outgas overly, FIB
should be fairly straightforward to 'drop' cross-sections at various points
in the mass down to 20 microns or so.

Interestingly, if ultrathin ( {200 nm) sections were needed for TEM
examination, ultramicrotomy could be used, though not easily. However,
micron thick sections of largish hard particles in the aggregate would
probably be too much for even a histoknife.

I would try and find a FIB.

Tom

Dr. Tom Malis
Science Advisor
Mineral Technology Branch
Natural Resources Canada
555 Booth St., Ottawa, Ontario
613-995-7358
malis-at-nrcan.gc.ca


} From: "Monson, Frederick C." {fmonson-at-wcupa.edu}
} Date: Fri, 26 Jul 2002 09:44:20 -0400
} To: "'List-Microscopy'" {Microscopy-at-sparc5.microscopy.com}
} Subject: FW: soil aggregates
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I forward this thread to the Microscopy list in the hope of getting more of
} a response for these folks that I was able to provide. If that is true, I
} think they will appreciate the help. I like the part about sectioning dust
} in sulfur (cake I suppose) and realized that most of us, who have delved
} into the mysteries of infiltrating and embedding, have spent some time
} sectioning 'dust' embedded in paraffin or plastic of one sort or another
} whether we knew it at the time or not. Knowing that dust is in and on
} everything we work with, breath and eat makes one consider the advisability
} of moving to a bubble combo of workshop and home. Well, not immediately.
}
} The needy are: MaryLou: mim11-at-cornell.edu and Bob Schoonhoven:
} rschoon-at-email.unc.edu
}
} Hope there's some real dirty expertise out there. It looks like we're in
} the loop for a real ESEM here, and I better start learning about what to
} expect from dirt and soil in my microscope.
}
} Thanks and for those going, have fun in Quebec.
}
} Fred Monson
}
} } ----------
} } From: MaryLou
} } Sent: Friday, July 26, 2002 7:08 AM
} } To: Monson, Frederick C.
} } Cc: histonet-at-pathology.swmed.edu
} } Subject: RE: soil aggregates
} }
} }
} } Good Morning Fred,
} }
} } Your internet searching capabilities are astounding. I thank you extremely
} }
} } much for all the sites you sent. This should keep the dirt people happy
} } for a long time. While I enjoy challenges, this one was just a tad out of
} }
} } my realm. The critter eyes are piling up and I must get back to the
} } safety
} } of paraffin :)
} }
} } Thanks again,
} } Mary Lou
} }
} }
} }
} } At 02:35 PM 7/25/2002 -0400, you wrote:
} } } Mary Lou,
} } }
} } } You sound like one who has been working this area, but you are
} } } asking a question that I would consider elementary for a material complex
} } } such as soil. Thus, I did my usual and instituted a search to see what I
} } } could find, because I am likely to see similar questions within the near
} } } future.
} } }
} } } For me, a real beginner, I think I will spend time here, and then start
} } } conversing with the experts whose methods are cited.
} } }
} } } http://www.soils.org/divs/s9/micromorph/micro.html
} } }
} } } From what I see, most of what workers in dirt consider "thin sectioning"
} } } involves grinding and/or sawing. When I first had to work with a truly
} } hard
} } } substance, I immediately found myself in the domain of the material
} } } scientist. Geologists don't ordinarily consider cutting a thin section
} } as
} } } we do, they think of grinding one - just like the ground bone sections
} } that
} } } one finds in almost all elementary histology slide sets.
} } }
} } } Aggregates of micro-size particles can be mounted and ground, if they are
} } on
} } } the macro- side of micro-. If smaller, and it is paramount that the
} } } aggregates NOT be disturbed, then I would turn, as rapidly as possible,
} } to
} } } more esoteric methods such as ion or plasma etching which can be used on
} } } embedded material and can, apparently be very productive.
} } }
} } } Here are a couple sites that might help to present the degree to which
} } } technology using electron optics and focused ion beams or plasmas are
} } used
} } } in both analysis and production.
} } }
} } } http://www.mrsec.harvard.edu/facilities.html
} } }
} } } http://www.mmc.or.jp/std/5.htm [this is a gas!]
} } }
} } } http://www.feicompany.com/eng/data/trimming.html
} } }
} } } Finally, Ion Beam Milling at,
} } }
} } } http://www.ionbeammilling.com/IONBEAMMILLINGILLUSTRETION.htm
} } }
} } }
} } } Hope this helps,
} } }
} } } Fred Monson
} } }
} } } Frederick C. Monson, PhD
} } } Center for Advanced Scientific Imaging
} } } Schmucker II Science Center
} } } West Chester University
} } } South Church Street and Rosedale
} } } West Chester, Pennsylvania, USA, 19383
} } } Phone: 610-738-0437
} } } FAX: 610-738-0437
} } } fmonson-at-wcupa.edu
} } } CASI URL: http://darwin.wcupa.edu/casi/
} } } WCUPA URL: http://www.wcupa.edu/
} } } Visitors URL: http://www.wcupa.edu/_visitors/
} } }
} } } } ----------
} } } } From: MaryLou
} } } } Sent: Wednesday, July 24, 2002 12:31 PM
} } } } To: histonet-at-pathology.swmed.edu
} } } } Cc: dawit Solomon
} } } } Subject: soil aggregates
} } } }
} } } } Dear Histonetters,
} } } }
} } } } A colleague is wanting to see inside soil aggregates of varying
} } } } thicknesses, up to several hundred microns. I was able to make
} } paraffin
} } } } sections, 20 microns, by soaking the samples in paraffin for many
} } } } hours. No solvents allowed. A researcher at NASA gets 1 micron
} } sections
} } } } from his dust particles in sulfur. We have no idea how he does it.
} } } } Thinner is better. Any suggestions out there? Do any bone grinders
} } have
} } } } any
} } } } ideas? Do you know of anybody else we can ask?
} } } } Please include Dawit in your responses. Dawit, do you have anything to
} } } } add?
} } } }
} } } } Thank you very much.
} } } } Mary Lou
} } } }
} } } }
} } } }
} }
} }



From daemon Sun Jul 28 17:02:44 2002



From: stiernberg-at-oregoncoast.com (by way of MicroscopyListserver)
Date: Sun, 28 Jul 2002 16:02:42 -0500
Subject: Ask-A-Microscopist: LM Leitz Dialux

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (stiernberg-at-oregoncoast.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday, July
28, 2002 at 12:51:43
---------------------------------------------------------------------------

Email: stiernberg-at-oregoncoast.com
Name: Ed Stiernberg

Organization: retired, 72 years old, checked grad school tho not in one

Education: Graduate College

Location: Tillamook, Oregon, USA

Question: For some 30 years, I have used a Leitz Dialux for pollen
grain work, utilizing mainly the 40X planapo objective and the 602
condenser. Recently, I saw a Dialux with what looks like a Berek
condenser. Please let me know how to obtain information on this
other type of condenser. I want to know if it is chosen for some
special work, how it differs from the more common 602, and the
practical principles underlying the two diaphragms with which it is
equipped.

---------------------------------------------------------------------------


From daemon Mon Jul 29 07:49:37 2002



From: Joanne Marrison :      jlm2-at-YORK.AC.UK
Date: Mon, 29 Jul 2002 13:40:42 +0100
Subject: Surplus Leica Ultracut Ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
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We have a Leica Ultracut Ultramicrotome surplus to
requirements and free to a good home.
If anyone is interested please contact Meg Stark on
ms1-at-york.ac.uk.

J Marrison
Department of Biology
University of York
Heslington
York
YO10 5DD




From daemon Mon Jul 29 08:13:05 2002



From: Rafal Dunin-Borkowski :      red10-at-cam.ac.uk
Date: Mon, 29 Jul 2002 14:04:41 +0100
Subject: TEM - PostDoc in Cambridge from October 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Post-Doctoral Research Assistant

Electron Holography of Semiconductor Devices

Department of Materials Science and Metallurgy
University of Cambridge, UK

A Post-Doctoral Research Assistant position is available from 1 October
2002 to pursue a programme of research into the development of electron
holography of semiconductor devices, with a particular emphasis on the use
of a novel electrical biasing holder to pass electrical currents through
samples as they are examined in the TEM.

The successful applicant will join a small team at Cambridge developing
electron holography for the characterisation of both electric and magnetic
fields in nanostructured materials. The research will be carried out in
conjunction with the Universities of Surrey and Limerick, as well as with
industry and with collaborators outside the UK.

It is essential that applicants have experience of advanced transmission
electron microscopy and image processing with a background in an
experimental physical science. Experience of electron holography is
desirable but not essential.

The position is funded by the EPSRC and is for 2 years in the first
instance. The starting salary will be on the University RA1A scale (£17,626
- £26,491).

Informal enquiries can be made to Dr Paul Midgley, Tel. +44 (0)1223 334561,
E-mail: pam33-at-cam.ac.uk or to Dr Rafal Dunin-Borkowski, Tel. +44 (0)1223
334564, E-mail: rafal.db-at-msm.cam.ac.uk.

Applications should be sent to Dr P.A. Midgley, Department of Materials
Science and Metallurgy, University of Cambridge, Pembroke Street,
Cambridge, CB2 3QZ, UK, and should include a full CV with the names and
addresses of two referees.

Closing date: 31 Aug 2002.



From daemon Mon Jul 29 14:01:20 2002



From: Armando Verdugo :      aeverdugo-at-alliedhightech.com
Date: Mon, 29 Jul 2002 11:47:09 -0700
Subject: GaAs Hazards

Contents Retrieved from Microscopy Listserver Archives
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Hello listers,

I need to gather information on the toxicity and possible health risks that
may be encountered by my technicians in processing (i.e. Cross-Sectioning,
TEM/Pre-FIB, Backside Grinding, etc.) GaAs die sections in our applications
laboratory.

Any policies from other labs would be appreciated, as well as points of
reference.

Thank you all in advance.

Regards,

Armando Verdugo
Laboratory Supervisor
(800) 675-1118 US and Canada
(310) 635-2466 Worldwide
(310) 762-6808 Fax


Visit our New Website!

www.alliedhightech.com


From daemon Mon Jul 29 15:16:27 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Mon, 29 Jul 2002 16:06:18 -0400
Subject: Semiconductor Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I need to analyze Si wafers for: Trench depth, Oxide thickness, Step height.
The pitch is 1, 5, and 20 um.
Does any one have suggestions how to proceed with the analysis.

Thanks in advance,

Pavel



From daemon Mon Jul 29 19:07:36 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 29 Jul 2002 16:45:07 -0700
Subject: Re: Semiconductor Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 01:06 PM 7/29/2002, you wrote:

} Hi,
} I need to analyze Si wafers for: Trench depth, Oxide thickness, Step height.
} The pitch is 1, 5, and 20 um.
} Does any one have suggestions how to proceed with the analysis.
}
} Thanks in advance,
}
} Pavel

Could you explain your situation a bit more? What type of
technology is being used? Is this damascene or SOG/LPCVD
etch-back planarization? Are you interested in cross section
or top-down? Any other data? Like number of metal layers,
type of metal, number of poly layers, minimum feature size.

gary g.



From daemon Mon Jul 29 19:07:36 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 29 Jul 2002 17:55:19 -0600
Subject: GaAs Hazards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I just did a quick check on the OSHA web site (www.osha.gov), but the only
hits were GaAs in connection with lasers and laser safety.

I did find a Materials Data Safety Sheet for GaAs:
http://www.wafertech.co.uk/msds/msds_gaas.html

and: http://www.nd.edu/~astuckey/MSDS/GaAs.htm

There were more hits relating to toxicity, but mostly dealt with Arsenic
toxicity. Go to Yahoo and search for "GaAs toxicity" for more information.

We used some pretty ugly stuff to prepare GaAs (Bromine-Methanol), and I
would be concerned about those things at least as much as about the GaAs
itself.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Armando Verdugo [mailto:aeverdugo-at-alliedhightech.com]
Sent: Monday, July 29, 2002 12:47 PM
To: Microscopy-at-sparc5.microscopy.com


Hello listers,

I need to gather information on the toxicity and possible health risks that
may be encountered by my technicians in processing (i.e. Cross-Sectioning,
TEM/Pre-FIB, Backside Grinding, etc.) GaAs die sections in our applications
laboratory.

Any policies from other labs would be appreciated, as well as points of
reference.

Thank you all in advance.

Regards,

Armando Verdugo
Laboratory Supervisor
(800) 675-1118 US and Canada
(310) 635-2466 Worldwide
(310) 762-6808 Fax


Visit our New Website!

www.alliedhightech.com


From daemon Mon Jul 29 22:10:08 2002



From: Tran Quang Huy :      tranquanghuy78-at-yahoo.com
Date: Mon, 29 Jul 2002 19:54:13 -0700 (PDT)
Subject: Detect dengue viruses type 4 by using immuno electron microscopy ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Virologists and Micoscopists,
I am a student (senior), I'm realizing the topic "
Research dengue viruses type 4 on Mosquito cells
C6/36". I used TEM to detect dengue viruses by using
immuno electron microscopy immuno gold conjugate but
without success. I tried many times. It was too
difficult to select antibodies and antibodies molarity
for dengue viruses type 4. I know you have a lot of
experienes to detect viruses. Please show me the
methods to realize the immuno electron microscopy
technics to detect dengue viruses type 4.
Thanks you
Tran Quang Huy



__________________________________________________
Do You Yahoo!?
Yahoo! Health - Feel better, live better
http://health.yahoo.com


From daemon Tue Jul 30 08:50:15 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Tue, 30 Jul 2002 09:42:14 -0400
Subject: Re: Semiconductor Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,
I do not really know what type of semiconductors these are, but looking on
SEM I think it is silicon based with Si nitride and oxide steps. EDS does
not pick up any metals. Looking at the cross section on a light microscope
at 1000x I can see the steps, but edge is not good enough to take
measurements of the step height using SEM. Is there any way to get edge
retention or some other techniques to get the measurements. I would also
prefer to get photos of the features.

Richard Beanland mentioned cleaving the wafer. Is this would get me good
enough edge to do the measurements and if it would , how do I cleave through
{110} plane? The steps are about 0.5 - 0.25um high. I would also try HF to
get some contrast.

Thank you,
Pavel





From daemon Tue Jul 30 09:10:18 2002



From: Joanne Marrison :      jlm2-at-YORK.AC.UK
Date: Tue, 30 Jul 2002 15:03:56 +0100
Subject: Surplus Leica Ultracut Ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

Many thanks for all your interest - the microtome has now
found a new home.

Regards
J Marrison

--
Dept of Biology
University of York
Heslington
York, UK
YO10 5DD



From daemon Tue Jul 30 10:34:08 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 30 Jul 2002 11:31:30 -0400
Subject: GaAs Hazards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike & Co.;

What is of concern with GaAs is fine particulates, the type that can get
deep into the lungs [.5um to 2 uM]. I'm not sure if the As disassociates
itself from the Ga once inhaled and in the mucus of lung tissue. Backside
grinding/polishing can be an issue since most processes that lap and grind
create a slurry and ultimately a dried particulate source for inhalation. I
don't think OSHA has a standard for the compound as such, just As alone.

As far as FIB is concerned, I see no issue with that since the area an FIB
can mill is miniscule.

This listing is of particular interest to me since we make GaAs micro
devices.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Mike Bode [mailto:mb-at-Soft-Imaging.com]
Sent: Monday, July 29, 2002 7:55 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


I just did a quick check on the OSHA web site (www.osha.gov), but the only
hits were GaAs in connection with lasers and laser safety.

I did find a Materials Data Safety Sheet for GaAs:
http://www.wafertech.co.uk/msds/msds_gaas.html

and: http://www.nd.edu/~astuckey/MSDS/GaAs.htm

There were more hits relating to toxicity, but mostly dealt with Arsenic
toxicity. Go to Yahoo and search for "GaAs toxicity" for more information.

We used some pretty ugly stuff to prepare GaAs (Bromine-Methanol), and I
would be concerned about those things at least as much as about the GaAs
itself.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Armando Verdugo [mailto:aeverdugo-at-alliedhightech.com]
Sent: Monday, July 29, 2002 12:47 PM
To: Microscopy-at-sparc5.microscopy.com


Hello listers,

I need to gather information on the toxicity and possible health risks that
may be encountered by my technicians in processing (i.e. Cross-Sectioning,
TEM/Pre-FIB, Backside Grinding, etc.) GaAs die sections in our applications
laboratory.

Any policies from other labs would be appreciated, as well as points of
reference.

Thank you all in advance.

Regards,

Armando Verdugo
Laboratory Supervisor
(800) 675-1118 US and Canada
(310) 635-2466 Worldwide
(310) 762-6808 Fax


Visit our New Website!

www.alliedhightech.com


From daemon Tue Jul 30 10:38:53 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 30 Jul 2002 11:38:17 -0400
Subject: Re: Semiconductor Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pavel;

If you have a "TallySurf" device, you can measure step height easily with it
and we use it routinely [accuracy within 20 angstroms]. However, you need
to state what the step height is, or what you think it is, for a method to
be chosen. The easiest method, provided you have a good SEM [FESEM is
best], is simply cleaving the wafer at a right angle across the step, trench
or oxide of interest and view it on edge. FIB takes too long and does not
give you quick multiple looks or the variance across the wafer easily. The
SEM will also let you see wall angles and the trench profile. Optical
methods alone may be misleading unless these are very large feautres. All
of this assumes a destructive analysis and you aren't sticking the wafer
back in your production line.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Monday, July 29, 2002 7:45 PM
To: ATC SEM Laboratory
Cc: MSA listserver


At 01:06 PM 7/29/2002, you wrote:

} Hi,
} I need to analyze Si wafers for: Trench depth, Oxide thickness, Step
height.
} The pitch is 1, 5, and 20 um.
} Does any one have suggestions how to proceed with the analysis.
}
} Thanks in advance,
}
} Pavel

Could you explain your situation a bit more? What type of
technology is being used? Is this damascene or SOG/LPCVD
etch-back planarization? Are you interested in cross section
or top-down? Any other data? Like number of metal layers,
type of metal, number of poly layers, minimum feature size.

gary g.



From daemon Tue Jul 30 12:59:43 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 30 Jul 2002 09:34:22 -0700
Subject: Re: Licensing policies for use of photomicrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Rosemary,
I have never had any requests to use any of my micrographs, although I have
found several used without my permission by a vendor selling printers at the
M&M show. He downloaded them from a web site that did have my permission. My
policy has always been that public funds pay for my facility, therefore the
micrographs I take are public property. It is a courtesy to acknowledge the
creator of the image. Any of my users can do what they like with their
pictures and my commercial customers have rights over images I take for them.
At 04:30 PM 07/24/2002 -0700, you wrote:
}
} Dear Listers,
} I would like to know of any licensing policies and procedures used by
} university EM and/or imaging facilities to handle requests to use
} photomicrographs.
} Thanks once again for your input.
} Rosemary
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Jul 30 13:30:22 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 30 Jul 2002 11:23:12 -0700
Subject: Re: GaAs Hazards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Armando,
When I had to organize the cleanup of a crystal-growing room that had left a
lot of GaAs dust around, I looked up the MSDS in order to devise a cleanup
protocol. The arsenic is only a problem if the material is acidified, so we
mopped up with a little soap and water and discarded the wet paper towels
and protective clothing as comtaminated waste. GaAs is fairly inert, but I
would avoid allowing any dust out of your cutting or polishing operations.
Clean up any fragments or dust and treat as any other arsenic-containing
compound: avoid inhalation and skin contact.
At 11:47 AM 07/29/2002 -0700, you wrote:

} Hello listers,
}
} I need to gather information on the toxicity and possible health risks that
} may be encountered by my technicians in processing (i.e. Cross-Sectioning,
} TEM/Pre-FIB, Backside Grinding, etc.) GaAs die sections in our applications
} laboratory.
}
} Any policies from other labs would be appreciated, as well as points of
} reference.
}
} Thank you all in advance.
}
} Regards,
}
} Armando Verdugo
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Jul 30 13:53:08 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Tue, 30 Jul 2002 11:44:13 -0400
Subject: Re: Semiconductor Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 7/30/02 9:42 AM, ATC SEM Laboratory at atcsem-at-earthlink.net wrote:

} I do not really know what type of semiconductors these are, but looking on
} SEM I think it is silicon based with Si nitride and oxide steps. EDS does
} not pick up any metals. Looking at the cross section on a light microscope
} at 1000x I can see the steps, but edge is not good enough to take
} measurements of the step height using SEM. Is there any way to get edge
} retention or some other techniques to get the measurements. I would also
} prefer to get photos of the features.
}
} Richard Beanland mentioned cleaving the wafer. Is this would get me good
} enough edge to do the measurements and if it would , how do I cleave through
} {110} plane? The steps are about 0.5 - 0.25um high. I would also try HF to
} get some contrast.
}
Dear Pavel,
How about evaporating a heavy metal from a point source at a
well-defined angle. By measuring the shadows and the orientation of the
steps with respect to the shadowing direction you could calculate their
size. Do you need more accuracy than you could get by this technique?
Yours,
Bill Tivol



From daemon Tue Jul 30 14:09:35 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 30 Jul 2002 14:54:39 -0400
Subject: Ultracut E service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
A colleague of mine just moved to the NIH and has inherited a
less-than-functional Reichert ultracut E. Its missing things like
the chuck holder & knife stage! If anyone can give him the name of a
service person in his "neighborhood" please send that info to:
Patrick Nahirney ata nahirnep-at-mail.nih.gov

Thanks,
Hope to see many of you in Quebec,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Jul 30 14:32:12 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Tue, 30 Jul 2002 15:21:23 -0400
Subject: TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have 3 set of boxes and film holders for 3 1/4 x 4" from a JEOL 100C that
I will give away to anyone. You just have to pay for shipping.

Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Tue Jul 30 17:06:18 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 30 Jul 2002 18:01:48 -0400
Subject: Re: Semiconductor Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pavel;

I saw your image and you have what you need right there. That image looks
like a cleave, yes? Doesn't look like an FIB did that. Why you don't detect
aluminum is beyond me. Are you sure your EDS is functioning?? Looks like
the Al metal is almost as thick as the phospho-silicate glass passivation.
Where's the scale bar on the SEM pic?

Details please.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: ATC SEM Laboratory [mailto:atcsem-at-earthlink.net]
Sent: Tuesday, July 30, 2002 9:42 AM
To: Microscopy-at-sparc5.microscopy.com


Gary,
I do not really know what type of semiconductors these are, but looking on
SEM I think it is silicon based with Si nitride and oxide steps. EDS does
not pick up any metals. Looking at the cross section on a light microscope
at 1000x I can see the steps, but edge is not good enough to take
measurements of the step height using SEM. Is there any way to get edge
retention or some other techniques to get the measurements. I would also
prefer to get photos of the features.

Richard Beanland mentioned cleaving the wafer. Is this would get me good
enough edge to do the measurements and if it would , how do I cleave through
{110} plane? The steps are about 0.5 - 0.25um high. I would also try HF to
get some contrast.

Thank you,
Pavel





From daemon Tue Jul 30 19:34:47 2002



From: ahmad.yekta-at-imaging.brocku.ca (by way of MicroscopyListserver)
Date: Tue, 30 Jul 2002 19:17:57 -0500
Subject: Ask-A-Microscopist: Reference Books/Materials Fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ahmad.yekta-at-imaging.brocku.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
July 30, 2002 at 14:26:32
---------------------------------------------------------------------------

Email: ahmad.yekta-at-imaging.brocku.ca
Name: Ahmad Yekta

Organization: Imaging Research

Education: Graduate College

Location: St Catharines, Ontario, Canada

Question: I would like to know about a few good references on the
subject of calibration and standardization of fluorescence
microscopes. E.g., uniformity of measuring field, sensitivity,...

---------------------------------------------------------------------------


From daemon Wed Jul 31 02:07:38 2002



From: moritz-andreas.meyer-at-amd.com
Date: Wed, 31 Jul 2002 08:58:12 +0200
Subject: Temperature range for EPOXY BOND 110

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I need to heat up samples to around 400°C for in-situ experiments. To hold the samples I want to glue them in place. Does anyone have any experience with EPOXY BOND 110 from TEDPELLA ? What temperature can it take before it desintegrates ? Or is there a good high temperature adhesive that you can recommend.

Thanks for the help in advance.

Andreas Meyer.

Moritz Andreas Meyer
Dipl.-Ing. (FH), MSc.
Materials Analyst
Materials Analysis Laboratory

AMD Saxony Manufacturing GmbH
Wilschdorfer Landstraße 101
M/S E32-MA
D-01109 Dresden
F. R. Germany

Tel. +49-351-277-4149
Fax +49-351-277-9-4149
moritz-andreas.meyer-at-amd.com






From daemon Wed Jul 31 03:00:36 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Wed, 31 Jul 2002 08:53:39 +0100
Subject: Semiconductor Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Richard Beanland mentioned cleaving the wafer. Is this would get me good
} enough edge to do the measurements and if it would , how do I cleave
through
} {110} plane? The steps are about 0.5 - 0.25um high. I would also try HF to
} get some contrast.

Pavel,
Just to enlarge on cleaving somewhat. I tend to find that the best
{110} cleaves are done without supporting the substrate (i.e. not breaking
it over an edge such as a glass slide or ruler). Here is a description of
what I have found to work - it would take about 30 seconds to demonstrate,
but is rather more difficult to describe without even a picture!
Draw a single line on the top of the wafer with a diamond scriber (about
2mm long, at the edge of the wafer), lined up with the region you want to
cleave through. Then hold the wafer between thumb and forefinger in both
hands, with the scribe mark on top between your thumbs. Break the wafer by
bending the sides downwards, trying to keep the force at the edge of the
wafer where the scribe mark is. If it works, the wafer should cleave very
easily with a dull 'dink'. If you haven't drawn a good single scribe line
then you can end up with a shower of fragments, particularly with big III-V
wafers which have high defect densities - safety glasses and an area where
you can retrieve the thousands of shards might be a good idea until you get
the hang of it! It is difficult to cleave off less than a cm of material
using this technique and get a good edge, and not easy to cleave through
small regions. If you have a small amount of material or small regions of
interest, gluing a stack of material followed by careful grinding and
polishing will be more sucessful. Ion milling the polished or cleaved
surface is also nesessary for good measurements of metal layers since they
will deform plastically during cleaving or polishing.

Good luck,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com



-----Original Message-----
} From: ATC SEM Laboratory [mailto:atcsem-at-earthlink.net]
Sent: 29 July 2002 21:06
To: Microscopy-at-sparc5.microscopy.com


Hi,
I need to analyze Si wafers for: Trench depth, Oxide thickness, Step height.
The pitch is 1, 5, and 20 um.
Does any one have suggestions how to proceed with the analysis.

Thanks in advance,

Pavel



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From daemon Wed Jul 31 03:46:22 2002



From: Alan Bright :      bright-at-dial.pipex.com
Date: Tue, 30 Jul 2002 15:10:05 +0100
Subject: microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Paul,

I think I can help you here, please send me a small sample so that I can do
some tests. I will then return the results with my findings.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com


-----Original Message-----
} From: "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com
[mailto:"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com]
Sent: 24 July 2002 21:19
To: Microscopy-at-sparc5.microscopy.com
Cc: Bob.Thompson/KRDC-at-alcan.com


We need to cut "thick?" cross sections of polymer/metal laminates for FTIR
microscope analysis.
Material thickness is around 100 microns..(section thickness somewhere
around 0.5 to 1mm i think)
I have 2 ultra microtomes but the sample size and section thickness
obtained from the ultramicrotome are to small for this application.

Any suggestions on the type of microtome i should consider. (Used is a
definite option)
or is there another piece of equipment i can use for this?

We are currently slicing pieces of our sample with a razor blade but this
causes some smearing in the layers.
I am currently being courted by one vendor who is going to lend me a
microtome to try out.

Suggestions are greatly appreciated
Vendors please reply to me directly.

Cheers

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com




From daemon Wed Jul 31 05:16:20 2002



From: Alan Bright :      bright-at-dial.pipex.com
Date: Wed, 31 Jul 2002 10:27:47 +0100
Subject: microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear Paul,

I think I can help you here, please send me a small sample so that I can do
some tests. I will then return the results with my findings.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com


-----Original Message-----
} From: "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com
[mailto:"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com]
Sent: 24 July 2002 21:19
To: Microscopy-at-sparc5.microscopy.com
Cc: Bob.Thompson/KRDC-at-alcan.com


We need to cut "thick?" cross sections of polymer/metal laminates for FTIR
microscope analysis.
Material thickness is around 100 microns..(section thickness somewhere
around 0.5 to 1mm i think)
I have 2 ultra microtomes but the sample size and section thickness
obtained from the ultramicrotome are to small for this application.

Any suggestions on the type of microtome i should consider. (Used is a
definite option)
or is there another piece of equipment i can use for this?

We are currently slicing pieces of our sample with a razor blade but this
causes some smearing in the layers.
I am currently being courted by one vendor who is going to lend me a
microtome to try out.

Suggestions are greatly appreciated
Vendors please reply to me directly.

Cheers

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com




From daemon Wed Jul 31 07:34:21 2002



From: Ami Frank :      act105-at-psu.edu
Date: Wed, 31 Jul 2002 09:36:07 -0400
Subject: Temperature range for EPOXY BOND 110

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter,
I did not submitted any SEM images, just light photo with micron bar. Could
you e-mail them to me directly, so I would know what are you taking about.

Regards,
Pavel
atcsem-at-earthlink.net



----- Original Message -----
} From: "Peter Tomic" {PTomic-at-Anadigics.com}
To: "'ATC SEM Laboratory'" {atcsem-at-earthlink.net} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, July 30, 2002 6:01 PM


Andreas-

Epoxy Technology sells an epoxy that we found to work extremely well. It
is called Epo-Tex, and it lists its degradation temperature at 341 degrees
(TGA). I am not sure how close to 400 degrees you needed, but if this is
close enough, their contact phone number is 978-667-3805.

Ami Frank

******************************
Ami Frank
Department of Biology
Pennsylvania State University
University Park, PA 16802
Office: 814-865-1769
Fax: 814-865-6193
email: act105-at-psu.edu
******************************
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi all,

I need to heat up samples to around 400°C for in-situ experiments. To hold
the samples I want to glue them in place. Does anyone have any experience
with EPOXY BOND 110 from TEDPELLA ? What temperature can it take before it
desintegrates ? Or is there a good high temperature adhesive that you can
recommend.

Thanks for the help in advance.

Andreas Meyer.

Moritz Andreas Meyer
Dipl.-Ing. (FH), MSc.
Materials Analyst
Materials Analysis Laboratory

AMD Saxony Manufacturing GmbH
Wilschdorfer Landstraße 101
M/S E32-MA
D-01109 Dresden
F. R. Germany

Tel. +49-351-277-4149
Fax +49-351-277-9-4149
moritz-andreas.meyer-at-amd.com




From daemon Wed Jul 31 08:56:38 2002



From: Chaoying Ni :      cni-at-udel.edu
Date: Wed, 31 Jul 2002 09:49:58 -0400 (EDT)
Subject: Surplus 1-Philips 501 with windowless EDS & 2-complete EDXA 9800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listers,

We have a Philips 501 with windowless EDS, both working before being
dismantled, and also a complete 9800 EDXA system. Free for non-profit edus
or orgs, or best offer to coms. Please send me inquiries off-line. Thanks!

****************************************
Chaoying Ni, PhD
201 DuPont Hall
The W.M. Keck Electron Microscopy Facility
College of Engineering
University of Delaware
Newark, DE 19716

(302) 831-8354 (O); -2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************



From daemon Wed Jul 31 09:24:14 2002



From: Stephanie Tyndall :      stephanie.tyndall-at-uconn.edu
Date: Wed, 31 Jul 2002 10:18:30 -0400
Subject: vanishing membranes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my first attempt at TEM I lost all the membranes on cultured primary
hippocampal neurons. All cellular organelles were intact and had no apparent
distortion. Has anyone else experienced this and if so do you know what
causes the membranes to vanish?

Alternately I'd be very interested in any fixation protocols anyone has for
primary hippocampal neurons that have worked for them in the past. I am
particularly interested in observing synapses.

Thanks!
Stephanie



From daemon Wed Jul 31 09:42:40 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 31 Jul 2002 08:45:22 -0600
Subject: Re: GaAs Hazards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would definitely recommend some form of ventilation for all cutting and
polishing operations. When we dimpled GaAs samples for TEMs, it was
sometimes possible to smell the typical Garlic odor from the Arsenicoxide
(and no, I had not been out eating Garlic soup the day before). Although I
don't know at this time if Arsenicoxide is toxic and at what levels (not too
low, as I am still writing this), why take the chances.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Tuesday, July 30, 2002 12:23 PM
To: Armando Verdugo
Cc: Microscopy-at-sparc5.microscopy.com


Dear Armando,
When I had to organize the cleanup of a crystal-growing room that had left a
lot of GaAs dust around, I looked up the MSDS in order to devise a cleanup
protocol. The arsenic is only a problem if the material is acidified, so we
mopped up with a little soap and water and discarded the wet paper towels
and protective clothing as comtaminated waste. GaAs is fairly inert, but I
would avoid allowing any dust out of your cutting or polishing operations.
Clean up any fragments or dust and treat as any other arsenic-containing
compound: avoid inhalation and skin contact.
At 11:47 AM 07/29/2002 -0700, you wrote:

} Hello listers,
}
} I need to gather information on the toxicity and possible health risks that
} may be encountered by my technicians in processing (i.e. Cross-Sectioning,
} TEM/Pre-FIB, Backside Grinding, etc.) GaAs die sections in our applications
} laboratory.
}
} Any policies from other labs would be appreciated, as well as points of
} reference.
}
} Thank you all in advance.
}
} Regards,
}
} Armando Verdugo
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Jul 31 10:10:33 2002



From: Beth Gregory :      gregory-at-4pi.com
Date: Wed, 31 Jul 2002 10:59:56 -0400
Subject: room available at Hilton Quebec for M&M

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have an extra reservation. I'm not sure how easy it would be to change
the single to a double.

Room Occupancy Type(# of People): Single
Room Type: Deluxe Room
Smoking Preference: Non Smoking Room
Total Nights: 6
Dates: Check-In Date: 08/03/2002 - Check-Out Date: 08/09/2002
Price (per night) CAD 235.00

If you are interested, please reply directly to me, not the list, for
additional information. Beth




From daemon Wed Jul 31 10:58:21 2002



From: Armando Verdugo :      aeverdugo-at-alliedhightech.com
Date: Wed, 31 Jul 2002 08:53:34 -0700
Subject: Temperature range for EPOXY BOND 110

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mr. Meyer,

EpoxyBond 110 is a product of Allied High Tech Products, Inc.
(http://www.alliedhightech.com/mounting/mountingacce/).

It is capable of withstanding up to about 385 degrees Celsius.

Should you have any additional questions, please contact our Manager of
Technical Products at the number below.

Gary Liechty
Manager, Technical Products
Allied High Tech Products, Inc.
310-635-2466
gdliechty-at-alliedhightech.com


Best regards,

Armando Verdugo
Laboratory Supervisor
(800) 675-1118 US and Canada
(310) 635-2466 Worldwide
(310) 762-6808 Fax


Visit our New Website!

www.alliedhightech.com


-----Original Message-----
} From: "moritz-andreas.meyer-at-amd.com"-at-sparc5.microscopy.com
[mailto:"moritz-andreas.meyer-at-amd.com"-at-sparc5.microscopy.com]
Sent: Tuesday, July 30, 2002 11:58 PM
To: Microscopy-at-sparc5.microscopy.com


Hi all,

I need to heat up samples to around 400°C for in-situ experiments. To hold
the samples I want to glue them in place. Does anyone have any experience
with EPOXY BOND 110 from TEDPELLA ? What temperature can it take before it
desintegrates ? Or is there a good high temperature adhesive that you can
recommend.

Thanks for the help in advance.

Andreas Meyer.

Moritz Andreas Meyer
Dipl.-Ing. (FH), MSc.
Materials Analyst
Materials Analysis Laboratory

AMD Saxony Manufacturing GmbH
Wilschdorfer Landstraße 101
M/S E32-MA
D-01109 Dresden
F. R. Germany

Tel. +49-351-277-4149
Fax +49-351-277-9-4149
moritz-andreas.meyer-at-amd.com






From daemon Wed Jul 31 10:58:24 2002



From: Edgar Voelkl :      evoelkl-at-nline.com
Date: Wed, 31 Jul 2002 10:37:35 -0500
Subject: RE: Semiconductor Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to offer one additional non-destructive method. Using
interferometric or holographic methods would give a very good measurement
of the step height and with some care information about the profile of the
edge. There should be some Michelson or Tolansky interferometers around -
I am afraid though they are not being sold any longer.

Edgar

At 08:53 AM 7/31/2002 +0100, Richard Beanland wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


________________________
Dr. Edgar Voelkl
M&M2002 Program Chair
(LOA ORNL)

nLine Corp.
4150 Freidrich Lane, Suite A
Austin, TX 78744

TEL: (512) 440-7720 x133
FAX: (512) 447-2765
eVoelkl-at-nLine.com



From daemon Wed Jul 31 11:04:13 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 31 Jul 2002 11:58:03 -0400
Subject: Temperature range for EPOXY BOND 110

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you look in the first MRS TEM Sample Prep book (I think that it is Vol 115), there is an article there on using an adhesive from Ceramabond for high temperature in-situ cross section preparation. You can look up the adhesives on Ceramabond's web site. I am sorry that I don't have all of the particulars.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: "moritz-andreas.meyer-at-amd.com"-at-sparc5.microscopy.com
[mailto:"moritz-andreas.meyer-at-amd.com"-at-sparc5.microscopy.com]
Sent: Wednesday, July 31, 2002 2:58 AM
To: Microscopy-at-sparc5.microscopy.com


Hi all,

I need to heat up samples to around 400°C for in-situ experiments. To hold the samples I want to glue them in place. Does anyone have any experience with EPOXY BOND 110 from TEDPELLA ? What temperature can it take before it desintegrates ? Or is there a good high temperature adhesive that you can recommend.

Thanks for the help in advance.

Andreas Meyer.

Moritz Andreas Meyer
Dipl.-Ing. (FH), MSc.
Materials Analyst
Materials Analysis Laboratory

AMD Saxony Manufacturing GmbH
Wilschdorfer Landstraße 101
M/S E32-MA
D-01109 Dresden
F. R. Germany

Tel. +49-351-277-4149
Fax +49-351-277-9-4149
moritz-andreas.meyer-at-amd.com






From daemon Wed Jul 31 11:04:13 2002



From: Armando Verdugo :      aeverdugo-at-alliedhightech.com
Date: Wed, 31 Jul 2002 09:00:19 -0700
Subject: Re: GaAs Hazards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who provided very useful information!

Our laboratory generally has a very minimal amount of GaAs processing, but
it seems recently we have seen somewhat of an increase, hence the reason for
this request. In my experience the best way to protect from the hazards of
the material is to process the material wet and wear rubber gloves, which is
exactly what everyone has responded as a good method.

Again, many thanks.

Armando Verdugo
Laboratory Supervisor
(800) 675-1118 US and Canada
(310) 635-2466 Worldwide
(310) 762-6808 Fax


Visit our New Website!

www.alliedhightech.com


-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Tuesday, July 30, 2002 11:23 AM
To: Armando Verdugo
Cc: Microscopy-at-sparc5.microscopy.com


Dear Armando,
When I had to organize the cleanup of a crystal-growing room that had left a
lot of GaAs dust around, I looked up the MSDS in order to devise a cleanup
protocol. The arsenic is only a problem if the material is acidified, so we
mopped up with a little soap and water and discarded the wet paper towels
and protective clothing as comtaminated waste. GaAs is fairly inert, but I
would avoid allowing any dust out of your cutting or polishing operations.
Clean up any fragments or dust and treat as any other arsenic-containing
compound: avoid inhalation and skin contact.
At 11:47 AM 07/29/2002 -0700, you wrote:

} Hello listers,
}
} I need to gather information on the toxicity and possible health risks that
} may be encountered by my technicians in processing (i.e. Cross-Sectioning,
} TEM/Pre-FIB, Backside Grinding, etc.) GaAs die sections in our applications
} laboratory.
}
} Any policies from other labs would be appreciated, as well as points of
} reference.
}
} Thank you all in advance.
}
} Regards,
}
} Armando Verdugo
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Jul 31 14:39:56 2002



From: Wright, Cheryl W :      CWright-at-Sikorsky.com
Date: Wed, 31 Jul 2002 15:30:31 -0400
Subject: room available at Hilton Quebec for M&M

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


O U C H !!!!!!!!!!!!!! me

-----Original Message-----
} From: Beth Gregory [mailto:gregory-at-4pi.com]
Sent: Wednesday, July 31, 2002 11:00 AM
To: microscopy-at-sparc5.microscopy.com


I have an extra reservation. I'm not sure how easy it would be to change
the single to a double.

Room Occupancy Type(# of People): Single
Room Type: Deluxe Room
Smoking Preference: Non Smoking Room
Total Nights: 6
Dates: Check-In Date: 08/03/2002 - Check-Out Date: 08/09/2002
Price (per night) CAD 235.00

If you are interested, please reply directly to me, not the list, for
additional information. Beth




From daemon Wed Jul 31 15:07:46 2002



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Wed, 31 Jul 2002 16:00:45 -0400 (EDT)
Subject: Re: vanishing membranes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Stephanie,

I, too, have had trouble in the past with vanishing membranes in cell
monolayers, though not hippocampal cells. The remedy was to en bloc stain
in aqueous 3% uranyl acetate for 15 minutes prior to dehydration and then
do a rapid dehydration in a graded series of ethanol beginning with 50 %.
End the dehydration with just one treatment of 100 % EtOH and then go right
into the infiltration with increasing concentrations of epon mixed in EtOH.
Try to minimize the cells' exposure to EtOH which tends to leach out
membranes.

I hope this helps.

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu

On Wed, 31 Jul 2002, Stephanie Tyndall wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} In my first attempt at TEM I lost all the membranes on cultured primary
} hippocampal neurons. All cellular organelles were intact and had no apparent
} distortion. Has anyone else experienced this and if so do you know what
} causes the membranes to vanish?
}
} Alternately I'd be very interested in any fixation protocols anyone has for
} primary hippocampal neurons that have worked for them in the past. I am
} particularly interested in observing synapses.
}
} Thanks!
} Stephanie
}
}



From daemon Wed Jul 31 15:44:34 2002



From: David French :      d.french-at-det.csiro.au
Date: Thu, 01 Aug 2002 09:21:35 +1000
Subject: Cameca Camebax for Disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pavel;

Please accept my apology. I confused your optical images with an example
someone sent me of a cleaved MOS device.

Very sorry for the confusion.

Peter

-----Original Message-----
} From: ATC SEM Laboratory [mailto:atcsem-at-earthlink.net]
Sent: Wednesday, July 31, 2002 8:29 AM
To: Microscopy-at-sparc5.microscopy.com


Peter,
I did not submitted any SEM images, just light photo with micron bar. Could
you e-mail them to me directly, so I would know what are you taking about.

Regards,
Pavel
atcsem-at-earthlink.net



----- Original Message -----
} From: "Peter Tomic" {PTomic-at-Anadigics.com}
To: "'ATC SEM Laboratory'" {atcsem-at-earthlink.net} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, July 30, 2002 6:01 PM


Good morning All,
CSIRO at Sydney, Australia has a Cameca Camebax for disposal "as is where
is". The instrument was purchased in 1983 and has 4 WDS spectrometers, LN2
anticontamination cold trap, Argon anti-contamination leak unit and Kevex
7000 EDS unit. If interested please contact me for further details.
Instrument must be removed by end of August.

Regards,
David French
David French
Mineralogist/Geochemist

Mail Address:
CSIRO Energy Technology,
Lucas Heights Science and Technology Centre,
Private Mail Bag 7,
Bangor NSW2234 ,
Australia
Delivery Address
CSIRO Energy Technology,
Store, Building 2
Lucas Heights Science and Technology Centre,
New Illawarra Road
Lucas Heights NSW2234 ,
Australia
Phone direct: 61-2-9710-6879
XRD Laboratory 61-2-9710-6903
switch: 61-2-9710-6777
Fax: 61-2-9710-6800
E-mail: d.french-at-det.csiro.au


From daemon Wed Jul 31 18:31:59 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 31 Jul 2002 19:24:41 -0400
Subject: FW: vanishing membranes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Don't feel too bad Stephanie,
}
} On my first attempt at TEM, my hand was held, along with all others in the
} class, so that there would not be a variety of subcellular objects that
} disappeared from the same tissue in the same class. Learning was fun in a
} class mode, but the training didn't last if you read beyond the next
} paragraph.
}
} If you used the glutaraldehyde-osmium sequence and saw the tissue blacken,
} then the membranes should have been there, unless they were dissolved in
} the heat of the embedment. If the unbound osmium wasn't washed out of the
} tissue, the alcohol will not be nice. If you use 100% EtOH in the final
} dehydration, it might have been from a bottle that was opened by a lab
} mate last week and the cap wasn't tightened properly.
}
} Practice with embedments first without tissue. Kick everyone else out of
} the lab. Separate all of your solutions and reagents. Do not ever share
} space with people who do paraffin embedding! Get REALLY grumpy and
} obsessive about everything. Then, when your lab is just the way you want
} it, you can begin to accumulate successes.
}
} The real key is to avoid using any important tissue for at least the six
} months of concerted effort it takes to whip the protocol to its knees and
} make it obey!
}
} My first attempt on my own, using the same generic protocol used in the
} course I took, resulted in sections on which I couldn't see any
} recognizable structure in the nuclei in the tissue. Learning these
} 'simple and straight forward' methods can be much like living through
} fraternity hazing, even if you are female. When you finally believe you
} will never be able to do any of it, you will be just in front of the door
} to success.
}
} Don't ask for suggestions. Hide away in your lab. Get a good book on the
} method(s). Carefully look over your protocol and ask what should be
} happening with every step. Then try IT again and again. When you finally
} understand the step-by-step rationale of the process, you can probably
} troubleshoot your own problems. Even learning to mix the resins is
} difficult when you try it on your own. Just know that every step of the
} process has a reason existing and that every one of them will cause at
} least one angry moment in every tech's career.
}
} Wait until you get great sections and they run away from the grid while
} you chase them in the boat. Or they get lost during staining and aren't
} on the grid when you take them to the EM. Or you put them in one place
} and when you get to the TEM all the sections are folded over the edge of
} the grids you prepared. Or both the carbon film and sections disappear
} between the lab and the EM suite, but they reappear when you return to
} look for them. Or you finally get it all right and you can see them on
} the screen, and, on that one occasion, the EM has a hiccup and the beam
} burns a hole in your section big enough for a truck to drive through. Or
} all of your first set of negatives are overexposed or overdeveloped, or
} out of focus. Then someone hears that you know how to do EM and comes to
} ask you for help, after you've just lost an important piece of tissue down
} the drain. All of these impediments are intended by the EM god to make
} you tough and hard.
}
} Every part of the process conspires to taunt you, embarrass you in front
} of others, and get you frustrated.
}
} I decided to show my son last night how to change brakes on one of our
} cars. Of course, nothing went as it had on the previous 20 brake lining
} changes and he got to see his Dad chasing around trying to catch jumping
} springs and lost clamps and holes that couldn't be found. I was
} frustrated and embarrassed, but he learned a great lesson, one which I had
} forgotten. Don't let your Dad teach you how to do anything. There is a
} god who interferes every time. Unfortunately, I've not learned this
} lesson over the course of 'teaching' four children how to do this and
} that. Some procedures never relent in their desire to impede your
} progress. Preparing tissue for TEM is one of those processes that only
} gives in when another beginner shows up somewhere. All of a sudden it
} will get easier.
}
} Those of us who have been THERE are here to help you through the hard
} times, but we won't be able to tell you how to fix the plagues that beset
} you as you progress toward EM heaven. We probably won't be able to tell
} you where your membranes have gone either, or, even worse, why they refuse
} to show themselves after all the trouble you took to preserve them and
} show them off. The only thing that drives us all is the expectation that
} membranes are always there when we start with a piece of tissue. As a
} young research fellow once said to me after failing to get a reaction in
} rabbit tissue to a mouse monoclonal antibody against a human elastin
} epitope, "It is clear that the rabbit simply doesn't have any elastin."
} Sometimes I think he had a true understanding of the issue of unfounded
} expectations in science, but then I had only stained elastin as an
} anatomic constituent of rabbit tissue for years prior to hearing his
} assertion.
}
} So, Stephanie, we finally come to this most unkind question, the one rests
} at the nub of your problem. What evidence do you have that the cells on
} that plastic substrate ever had membranes?
}
} Please take only a few parts of this seriously. Which parts is a secret.
} And, if you would like to know why you have been subjected to this, it is
} because I have spent the afternoon wraslin' with a critical point dryer
} that works beautifully if one can get it closed before the specimen dries
} by other means than critical.
}
} Also, did you know that a reconstruction of a stack of optical sections
} will show moving particles in standard orbits as rings and those moving in
} arched paths as columns? This sort of thing merely suggests that one
} SHOULD probably get back to the old and reliable ways of looking at
} things: Fixed, immobilized and embedded, thin sectioned, stained with
} safe uranium and lead, illuminated by a beam of high energy electrons, and
} recorded on a piece of Mr. Kodak's fancy photo-sensitive celluloid.
}
} It couldn't be true, but here's a(n) (im)possibility. I asked the
} following on a test long ago. [Lord! Was I nasty as a youngster!!!]
} "Consider a 60nm section that is nowhere vertical through an interface
} between cell or cell constituent or cell embedment. Under such a
} condition, what would be the visible evidence of the bimolecular lipid
} leaflet (or membrane) which is so characteristically used to describe the
} plasma and other so-called 'membranes'?" The correct answer is "There
} would be None". The unasked part of the question is, "How likely is such
} a scenario?" or "How easily would you be to find evidence of a
} bimolecular lipid leaflet in a thin section of a 1mm cube of liver
} parenchymal cells?" "What would a stack of smooth endoplasmic reticulum
} with a 20nm interlamellar spacing look like if the stack were sectioned at
} an angle of 45 degrees to a line which was perpendicular to the lamellae
} of the array?"
}
} This is a short answer question that is worse. "The nucleus that is shown
} in this photomicrograph exists in a paraffin section of liver taken from a
} mouse. Please describe the molecular structure of the nuclear envelope as
} you see it in THIS nuclear profile in the section described." The
} correct answer, of course, is that after all that processing the lipid
} portion of the membrane is gone unless some part of it has been treated so
} that it remains. Osmium tetroxide reacts with unsaturated double bonds to
} preserve THOSE parts of lipid membranes, but even that is partly mystery.
}
} So, I have tried to answer your question with a few thoughts from a grumpy
} old man who has had a bad afternoon with a technology whose success is
} sometimes determined by a person named Serendipity. Critical point dryers
} are either constructed from brass pipe with 1" walls or tiny, thread-like
} copper pipes connected to stainless steel chambers and valves by BIG
} compression fittings. When such instruments go bad, they become
} inscrutable. None of this, however, helps you to find those miscreant
} membranes. Well, as I said above, it was unlikely that we would be able
} to find them for you. The gods don't work in THAT mysterious way. EVER!!
} When you lose your membranes, you are the only one who can find them, no
} matter where they have gone or why. The rest of us are only capable of
} asking questions that may or may not help in the search. You know, "Did
} you look under the bed?"
}
} Regards and sympathy,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} c/o Geology/Astronomy
} West Chester University
} South Church Street and Rosedale Ave
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
} ----------
} From: Stephanie Tyndall
} Sent: Wednesday, July 31, 2002 10:18 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: vanishing membranes
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} In my first attempt at TEM I lost all the membranes on cultured primary
} hippocampal neurons. All cellular organelles were intact and had no
} apparent
} distortion. Has anyone else experienced this and if so do you know what
} causes the membranes to vanish?
}
} Alternately I'd be very interested in any fixation protocols anyone has
} for
} primary hippocampal neurons that have worked for them in the past. I am
} particularly interested in observing synapses.
}
} Thanks!
} Stephanie
}
}
}
}


From daemon Wed Jul 31 18:37:33 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 31 Jul 2002 19:30:06 -0400
Subject: RE: microtome

Contents Retrieved from Microscopy Listserver Archives
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Wow! This is like having Walter McCrone offer to do your microscopy for
you. Lucky man! I hope we can hear about the solution to the problem.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Alan Bright
} Reply To: bright-at-dial.pipex.com
} Sent: Tuesday, July 30, 2002 10:10 AM
} To: "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com;
} Microscopy-at-sparc5.microscopy.com
} Cc: Bob.Thompson/KRDC-at-alcan.com
} Subject: RE: microtome
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Paul,
}
} I think I can help you here, please send me a small sample so that I can
} do
} some tests. I will then return the results with my findings.
}
} Best Regards
}
} Alan Bright
}
} Bright Instrument Co.Ltd.
} St Margaret's Way
} Huntingdon
} Cambridgeshire
} PE29 6EU
} England
}
} Tel No:+44 (0)1480 454528
} Fax No:+44 (0)1480 456031
} Email: AlanBright-at-brightinstruments.com
} Web Site: www.brightinstruments.com
}
}
} -----Original Message-----
} } From: "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com
} [mailto:"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com]
} Sent: 24 July 2002 21:19
} To: Microscopy-at-sparc5.microscopy.com
} Cc: Bob.Thompson/KRDC-at-alcan.com
} Subject: microtome
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We need to cut "thick?" cross sections of polymer/metal laminates for FTIR
} microscope analysis.
} Material thickness is around 100 microns..(section thickness somewhere
} around 0.5 to 1mm i think)
} I have 2 ultra microtomes but the sample size and section thickness
} obtained from the ultramicrotome are to small for this application.
}
} Any suggestions on the type of microtome i should consider. (Used is a
} definite option)
} or is there another piece of equipment i can use for this?
}
} We are currently slicing pieces of our sample with a razor blade but this
} causes some smearing in the layers.
} I am currently being courted by one vendor who is going to lend me a
} microtome to try out.
}
} Suggestions are greatly appreciated
} Vendors please reply to me directly.
}
} Cheers
}
} Paul D. Nolan
} Electron Optics
}
} Alcan International Limited
} Kingston Research and Development Centre
} P.O.Box 8400, 945 Princess Street
} Kingston, Ontario K7L 5L9
}
} Tel: (613) 541-2066
} Fax: (613) 541-2134
} paul.nolan-at-alcan.com
}
}
}
}


From daemon Thu Aug 1 03:38:00 2002



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Wed, 31 Jul 2002 15:51:38 -0700
Subject: Position--ASU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Post-Doctoral Research Associate

A post-doctoral research associate position is available within the Center
for High Resolution Electron Microscopy at Arizona State University. The
position is sponsored by a consortium of semiconductor companies and the
primary focus of the research will be the development and application of
electron holography to industrially relevant semiconductor devices. Areas
of particular interest include the study of lateral dopant diffusion and
the effect of thermal annealing treatment on dopant diffusion. Candidate
must have a Ph.D. in material science, or physics, with experience in
characterization of electronic devices using electron holography.
Experience with the techniques of high resolution imaging, and electron
energy loss spectroscopy is preferred. Please submit your resume together
and the names and addresses of 3 referees to: Professor David J. Smith,
Director, Center for Solid State Science, Arizona State University, Tempe,
AZ 85287-1704, Fax (480) 965-9004, email csss.director-at-asu.edu. AA/EOE

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Thu Aug 1 04:49:59 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Thu, 1 Aug 2002 10:41:23 +0100
Subject: Re: GaAs Hazards

Contents Retrieved from Microscopy Listserver Archives
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We recently did an environmental health & safety assessment looking at this,
although we were more concerned with the very toxic hydrides. None were
detected with a standard portable gas monitor (the type which clips on a
belt, used when changing As cylinders on MOVPE kits etc.) 3 inches above the
griding paper (1200 grit SiC, grinding about 1 cm^2 of GaAs at ~500 rpm in
continuous running water). I've never noticed a smell when grinding GaAs
(and I've been doing it for ten years!). InP is another story - a very
strong smell is noticable, and it was decided that fume extraction was a
good idea (a lot easier than holding your breath!). The dose without
ventilation was within UK occupation health limits as long as it was done
for less than 30 minutes/day. (This only applies to relatively coarse
grinding, where you're removing a lot of material. Polishing is okay.) As
Mike says, why take chances when there are solutions available.

Richard

____________________________________________________________________________
______
Mike Bode said:

I would definitely recommend some form of ventilation for all cutting and
polishing operations. When we dimpled GaAs samples for TEMs, it was
sometimes possible to smell the typical Garlic odor from the Arsenicoxide
(and no, I had not been out eating Garlic soup the day before). Although I
don't know at this time if Arsenicoxide is toxic and at what levels (not too
low, as I am still writing this), why take the chances.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Tuesday, July 30, 2002 12:23 PM
To: Armando Verdugo
Cc: Microscopy-at-sparc5.microscopy.com


Dear Armando,
When I had to organize the cleanup of a crystal-growing room that had left a
lot of GaAs dust around, I looked up the MSDS in order to devise a cleanup
protocol. The arsenic is only a problem if the material is acidified, so we
mopped up with a little soap and water and discarded the wet paper towels
and protective clothing as comtaminated waste. GaAs is fairly inert, but I
would avoid allowing any dust out of your cutting or polishing operations.
Clean up any fragments or dust and treat as any other arsenic-containing
compound: avoid inhalation and skin contact.
At 11:47 AM 07/29/2002 -0700, you wrote:

} Hello listers,
}
} I need to gather information on the toxicity and possible health risks that
} may be encountered by my technicians in processing (i.e. Cross-Sectioning,
} TEM/Pre-FIB, Backside Grinding, etc.) GaAs die sections in our applications
} laboratory.
}
} Any policies from other labs would be appreciated, as well as points of
} reference.
}
} Thank you all in advance.
}
} Regards,
}
} Armando Verdugo
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



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From daemon Thu Aug 1 08:06:05 2002



From: Barbara Maloney :      bmalon01-at-fiu.edu
Date: Thu, 01 Aug 2002 08:55:39 -0400
Subject: colloidial gold

Contents Retrieved from Microscopy Listserver Archives
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Dear Group - what is the preferred method to apply colloidial gold (40
nm) to silicon monoxide on formvar 200 mesh Cu grids? Right now I would
like to use the colloidial gold as a reference when imaging
nanoparticles. I have never used this colloidial gold before, so I was
surprised to see it in a liquid form.
Thanks
Barbara



From daemon Thu Aug 1 08:06:11 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Thu, 1 Aug 2002 09:01:43 -0400
Subject: vanishing membranes

Contents Retrieved from Microscopy Listserver Archives
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ok, as fred knows, i hate solving other peoples preoblems for them. i have
enough of my own. here goes: the fault is in one or more of the following
areas:
either to short a time in OsO4, that is the membranes were not stabilized.
to long a time in OsO4, it is rarely explained to newcomers, but OsO4 can
leach out cellural componets. no more than 1 hour with no more than 2% OsO4.

to long a dehydration in ethanol assuming that is what you are using.
no more than 10 minutes per dehydration step. for cells i always start at eith
50 ro 70%. always use a 2 15 min propylene oxide as an intermediate step.
finaly what is your embedding media? you sould be using an epon subsitute.

in all my 20+ years of doing EM i have never lost a single membrane. tissue
culture cells can be picky.

fred is correct, you need to take control of all your solutions. no one i
repeat no should be using the solutions you are using. that is asking for
trouble. always mix your own fixatives and embedding media, unless it is
someone you trust completely.

you should send us you embedding schdule. it will make figuring out whats
going on a lot simpler.
ok my carpel tunnel is acting up so enough typing. good luck EM really isn't
hard, it's just 100 times more excating that histo.
John Hoffpauir

PS: fred we are on the final plans for our scope room. hows yours going?





From daemon Thu Aug 1 10:56:05 2002



From: Barrister Ibe :      ibe_60-at-lawyer.com
Date: Thu, 01 Aug 2002 16:46:06
Subject: ASSISTANCE URGENTLY NEEDED

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ATTN:

With a heart full of tears I write to seek for your help at this time of grief in my family. I am the wife of Nigeria's former head of state, Late Gen. Sani Abacha [Alhaja Mariam Abacha].

Soon after the demise of my husband, the succeeding regime of Abdusallami Abubakar besieged my family and started probing every activity of my husband while in office. The present civilian government continued with probe to the extent of arresting my son Mohammed Abacha alongside with my husband's Chief Security Officer (CSO), major Hamza Mustapha. The two are currently facing trial in the court.

Several false allegation of murder of some
politician, Activists/prodemocracy agents; misgovernance and vast loot of the Nations Treasury were leveled against my husband. As
a result, all our properties have been seized and our Bank Account frozen leaving us with no means of li! velihood.

The only money left for us is a defaced cash sum of US$44M(Forty-Four Million U.S Dollars) contained In trunk boxes which I instructed my half brother to take out of the country immediately my husband died. The money has to be defaced in order to beat security operatives while on transit. My half brother was able to cross the box to Europe and deposited them with a Trust / Security Companys family Treasures before His death.

Right now, my family is in urgent need of having this money invested outside Nigeria because of several ongoing probes on ex-service men and also political instability in the country.

My request therefore is for you to give me necessary assistance to claim this money from the security company and transfer it to your personal account in your country. Our family lawyer will process the enabling documents in your name as the Trustee/Beneficiary of the fund to facilitate its withdrawal.

I am equally willing to compensat! e your effort with 30% of the money when it arrives your account.

Please note that government is still keeping
surveillance over the activities of my family with regards to traveling and Telephone calls. Therefore you should treat this business with absolute confidentiality. All your correspondence to me must be strictly through my e-mail address.

The lawyer as my representative would be meeting you in due course on my behalf as the need may arise. Please contact him on his mobile phone number: 234-803-327-9244

I assure you that no risk of any kind is involved in this transaction.

I eagerly await your immediate reply.


Thanks

Alhaja Mariam Abacha,
c/o Barrister Ibe


From daemon Thu Aug 1 11:17:20 2002



From: Barrister Ibe :      ibe_60-at-lawyer.com
Date: Thu, 01 Aug 2002 17:09:37
Subject: ASSISTANCE URGENTLY NEEDED

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ATTN:

With a heart full of tears I write to seek for your help at this time of grief in my family. I am the wife of Nigeria's former head of state, Late Gen. Sani Abacha [Alhaja Mariam Abacha].

Soon after the demise of my husband, the succeeding regime of Abdusallami Abubakar besieged my family and started probing every activity of my husband while in office. The present civilian government continued with probe to the extent of arresting my son Mohammed Abacha alongside with my husband's Chief Security Officer (CSO), major Hamza Mustapha. The two are currently facing trial in the court.

Several false allegation of murder of some
politician, Activists/prodemocracy agents; misgovernance and vast loot of the Nations Treasury were leveled against my husband. As
a result, all our properties have been seized and our Bank Account frozen leaving us with no means of li! velihood.

The only money left for us is a defaced cash sum of US$44M(Forty-Four Million U.S Dollars) contained In trunk boxes which I instructed my half brother to take out of the country immediately my husband died. The money has to be defaced in order to beat security operatives while on transit. My half brother was able to cross the box to Europe and deposited them with a Trust / Security Companys family Treasures before His death.

Right now, my family is in urgent need of having this money invested outside Nigeria because of several ongoing probes on ex-service men and also political instability in the country.

My request therefore is for you to give me necessary assistance to claim this money from the security company and transfer it to your personal account in your country. Our family lawyer will process the enabling documents in your name as the Trustee/Beneficiary of the fund to facilitate its withdrawal.

I am equally willing to compensat! e your effort with 30% of the money when it arrives your account.

Please note that government is still keeping
surveillance over the activities of my family with regards to traveling and Telephone calls. Therefore you should treat this business with absolute confidentiality. All your correspondence to me must be strictly through my e-mail address.

The lawyer as my representative would be meeting you in due course on my behalf as the need may arise. Please contact him on his mobile phone number: 234-803-327-9244

I assure you that no risk of any kind is involved in this transaction.

I eagerly await your immediate reply.


Thanks

Alhaja Mariam Abacha,
c/o Barrister Ibe


From daemon Thu Aug 1 11:49:15 2002



From: Amy McGough :      amcgough-at-bilbo.bio.purdue.edu
Date: Thu, 1 Aug 2002 11:46:24 -0600
Subject: Position: TEM lab manager (msa post)

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Electron Microscopy Laboratory Manager at Purdue University

An opening is now available for an energetic individual to manage a
multi-user electron microscopy facility in the Department of
Biological Sciences at Purdue University. The successful candidate
will have primary responsibility for the day-to-day operations of a
laboratory specializing in transmission electron cryomicroscopy of
biological macromolecules and their assemblies. Responsibilities
include:

Overseeing the routine maintenance and use of a multi-user
high-resolution transmission electron microscopy facility

Training new users in use of the microscopes and in specimen
preparation techniques for high-resolution imaging of biological
macromolecules

Evaluating equipment performance regularly to maintain maximum
performance standards

Working with faculty, students, and visiting scientists on scientific
experiments as time permits

Associates or higher degree and a minimum of 1 year of experience
working in and/or managing a transmission electron microscopy
laboratory required. Excellent written and oral communication skills
are essential. Computer skills are required. Successful candidate
will be trained in preparation and imaging techniques and necessary
diagnostic skills for high-resolution electron cryomicroscopy.

Purdue University is home to outstanding researchers in the
biological sciences as well as a world-reknown group of structural
biologists. The Department of Biological Sciences provides a
stimulating intellectual environment as well as outstanding modern
scientific facilities including FEI CM300-FEG, CM200-FEG, and EM420
electron cryomicroscopes dedicated to structural studies and an EM410
for routine biological work. Purdue is home to over 50,000 students,
faculty, and staff, and is located in West Lafayette, Indiana --
approximately 1 hour northwest of Indianapolis and 2 hours southeast
of Chicago. The area provides the advantages associated with a major
university while providing an affordable and attractive small town
environment in which to live.

Interested individuals should contact:
Prof. Amy McGough
Department of Biological Sciences
Purdue University
West Lafayette, IN 47907-1392 USA
fax: +1 (765) 496-1189

Purdue is an equal access/equal opportunity university



From daemon Thu Aug 1 12:41:26 2002



From: John R Reffner :      rsrj2r-at-rohmhaas.com
Date: Thu, 1 Aug 2002 13:32:47 -0400
Subject: Job Opening - Rohm and Haas Company

Contents Retrieved from Microscopy Listserver Archives
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JOB POSTING:
SENIOR SCIENTIST - MICROSCOPY

The Microscopy Group in the Analytical and Computational Technology Center
of the Rohm and Haas Company, one of the world's largest specialty chemical
companies, is seeking candidates for a senior scientist position. The
position is located at our Spring House site, near Philadelphia. The senior
scientist will use electron microscopy and related technologies included in
our microscopy lab (SEM/EDS, TEM, Optical, AFM, etc.) to study materials
from across the company including various polymers, coatings, colloids,
catalysts, electronics materials, and other complex systems. The senior
scientist will develop test methods, and explore new technologies or
combinations of technologies to address issues in our product research
departments. The senior scientist will often work in collaboration with
other analytical and product area scientists and have responsibility for
technical oversight of more routine work done by other researchers.

Applicants for this position must have a Ph.D. degree in Chemistry, Chemical
Engineering, Materials Science, or a closely related discipline, with
substantial experience in electron microscopy. Broader experience in other
experimental techniques for materials characterization is desirable.
Experience and theoretical knowledge in any of the following areas is
desirable: polymer science, colloid science, catalysis, XRF, Surface
Analysis, crystallography, and computer programming. The applicant must
have good communication skills, strong initiative, and be able to succeed in
a collaborative environment.


Rohm and Haas offers a highly competitive total compensation program
involving base salary calibrated against the market and variable pay
opportunity through an annual bonus program. Relocation assistance will be
provided. We are committed to the professional development of our
Technology staff. Candidates for this position should forward their resumes
to: Dr. John R. Reffner, Rohm and Haas Company, 727 Norristown Road, P.O.
Box 904, Spring House, PA 19477 or by fax to (215) 619-1607.

Rohm and Haas is an Equal Opportunity Employer.

NOTE: Interested candidates who will be at M&M 2002 can contact me during
the week to meet during the conference. I can be reached at 1-800-232-8691
x 5283 (best) or by leaving a message with the M&M message center or email
at e2jrr-at-iname.com




From daemon Thu Aug 1 12:52:54 2002



From: John Hoffpauir :      John.Hoffpauir-at-mail.tju.edu
Date: Thu, 1 Aug 2002 13:50:23 -0400
Subject: never cast stones re:tem samples-how long

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well jim it certainly took you long enough to reply.

phil is the one who told me about the diamond knives. if all three were ruined
then you ruined the most recently purchased knives. the LKB needed repair
which you never had done, why i can only guess.
as to the mess the lab was in, you sir completly trashed the lab. it was
orderly clean and a completely functional EM lab till you took over the
"bridge the gap" as you say. i am amazed if you were doing so well why did i
need to come back there on weekends to work on cases. as for the kind of EM
tech you were jim, i saw a few of your so called sections on the scope. i have
seen students with glass knives do better.

as for cleaning the lab jim. you sir were the last person working there.
explain that. if you can. evey weekend i came there i found more trash, more
mess and more instruments non functional. i remember coming in finding the
print processor completly non functional due to your neglagence. it took me
four hours just to clean it. you hadn't even taken the time to do that. i came
by the EM lab after Phil had take over. there were bottles all over the work
bench the lab rearrange, my guess is you were marking the territory, phil had
to use the table that you subsituted for the desk that was there to work on.

as for the LKB the holder looked like it had been droped or thown on the
floor. it had to be replaced with a used ultra cut, more expense to an already
cash straped hospital. way to go jim.
just so we are clear, phil was the one that told be the diamond knives were
trashed. so jim, perhaps you should take this up with him as well, my guess is
you don't have the stones for it. just like you didn't have them to call me or
talk with me about this or the mess YOU made of the lab.

i also remember seeing well over 30 strips of negatives just hanging there,
you hadn't even bothered to print them. half were screwed up. phil showed them
to me. perhaps you don't remember how. this is why an avg EM tech (you jim) is
a bad one.
in the future don't call me friend or weasel. i am neither.

just so you all know i did quit my clinical job where i was in good standing
and cinsidere to be a very good clinical tech. i completed every case handed
to me within 36 hours, including the DIF and printing the negatives.
i left the job for a research job where i had a chance to grow as a tech and
start up a core lab, with a new techni FEI tem. the job is challenging, and i
have been here for over 8 months. you couldn't even do the em job for three
months.
the person just so everyone here knows is jim wieldmann an cytology
supervisor.
i am john hoffpauir research assoc. jim, if you have the stones to challenge
me personaly here is my phone number 215 503 7019. other wise you only look
more foolish by writing emails.




From daemon Thu Aug 1 13:16:40 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Thu, 01 Aug 2002 11:00:58 -0700
Subject: Positions Available - Southern California

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


South Bay Technology, Inc., a leading Materials Processing Equipment
manufacturer, has several positions available including:

Field Sales Engineer

Applications Laboratory Technician

In-House Sales

All of these positions will be located in our Southern California
headquarters which is located midway between Los Angeles and San Diego
in the beachfront community of San Clemente, CA

For complete job descriptions, please contact:

Human Resources
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
FAX: +1-949-492-1499

Email: jobs-at-southbaytech.com

We will also have these jobs posted at the Microscopy & Microanalysis
Meeting in Quebec City. If you plan to be there, please visit us in
Booth 1032.

Best regards-

David
--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Thu Aug 1 16:11:05 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.princeton.edu
Date: Thu, 1 Aug 2002 16:58:27 -0400
Subject: Jeol film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear EM Community,
I've had a heavy response to this posting and so I've decided to
send 1 set of cassettes and boxes to 3 different educational institutions.
1 set to U of Penn, 1 set to Oklahoma State and 1 set to the Georgia
Institute of Technology. I believe this will be a better way to share the
resource.

Regards,

Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Thu Aug 1 17:26:51 2002



From: Biotech InMind :      team-at-biotech.inmind.info
Date: Thu, 01 Aug 2002 18:12:10 -0400
Subject: Biotechnology Industry Monitor You Shouldn't Be Without

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Biotechnology Professional:

In these challenging times, staying one step ahead of the competition in the biotechnology arena is crucial. If you are looking to gain leverage and insight, a unique, targeted industry monitoring tool and search engine -- Biotech InMind -- can help you achieve and maintain a competitive edge.

Biotech InMind focuses entirely on the biotechnology industry. It gathers, analyzes, and stores web pages from the leading biotechnology and related domains (manufacturers, startups, universities, R&D centers, consultants, service providers, etc.), monitors updates to the U.S. patent database, and accesses a database of more than 10,000 technology marketing reports. Unlike traditional search engines, Biotech InMind is tuned precisely to yield results that biotechnology industry professionals require. Since our entire database is refreshed almost daily, the information you receive is always current.

Biotech InMind offers a proprietary monitoring service that notifies you periodically when information that matches your profile is added to any of the pages Biotech InMind indexes, or when new patents or marketing reports are added. You can actually be updated within hours of any critical changes in intellectual property or any new marketing studies that would be of interest to your organization.

We'd like to invite you to experience the power of Biotech InMind free for 14 days. At the conclusion of the trial period, access to Biotech InMind can be purchased for only $49.95 per year. That’s less than $0.14 per day! We're confident that Biotech InMind will prove to be an invaluable tool and one of the best investments your company could make.

For a free 14 day trial membership, please click on the following link:

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If you have receive this email in error, or no longer wish to receive communications from Biotech InMind, please click the following link:

http://biotech.inmind.info/remove.asp?id=4286&vi=26289

Thoughtwise LLC.




From daemon Thu Aug 1 19:37:07 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 2 Aug 2002 12:27:41 +1200
Subject: JEOL 840A Optical microscope rotation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Do any of you ingenious types out there know if it is possible (and if it is,
please tell me how) to rotate the 840 OM about an axis radial to the
column so that the eyepiece points directly upwards rather than towards
the operator?

I am using a CCD video camera to look into the eyepiece, and such a
rotation would make it much easier to mount the camera.

It works well, incidentally, much more convenient to use than leaning
forward.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Aug 1 21:41:06 2002



From: JLCastner-at-aol.com
Date: Fri, 2 Aug 2002 00:25:35 EDT
Subject: Calculating magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

I have interceeded and blocked the rapidly escalating Email
flame/war on this Listserver by 2 individuals with
the subject line



Hello All,

Forgive me if my question is elementary, but I am just beginning to so
some simple brightfield photomicroscopy. Is there a simple formula for
calculating the total magnification achieved when a photo is printed? For
example, if you have 10X eyepieces and use a 40X objective when the photo is
exposed, and then print it to a size of 3" X 4" on a journal page, can you
easily calculate the final magnification?

Also, if such formulae do exist, were they developed with the assumption
that one is using a 35mm camera? I am using a Nikon D1X (their top of the
line digital). This camera has a chip, and that chip is different from the
one used in a Nikon Coolpix 990. The projector lens in the tube of my
compound microscope is 1.6X.

Thank you for any light anyone can shed on how to do these calculations.

Sincerely,

Jim Castner


From daemon Fri Aug 2 01:06:13 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 01 Aug 2002 22:56:04 -0400
Subject: Re: Calculating magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 8/2/02 12:25 AM, "JLCastner-at-aol.com"-at-sparc5.microscopy.com at
"JLCastner-at-aol.com"-at-sparc5.microscopy.com wrote:

}
} Forgive me if my question is elementary, but I am just beginning to so
} some simple brightfield photomicroscopy. Is there a simple formula for
} calculating the total magnification achieved when a photo is printed? For
} example, if you have 10X eyepieces and use a 40X objective when the photo is
} exposed, and then print it to a size of 3" X 4" on a journal page, can you
} easily calculate the final magnification?
}
} Also, if such formulae do exist, were they developed with the assumption
} that one is using a 35mm camera? I am using a Nikon D1X (their top of the
} line digital). This camera has a chip, and that chip is different from the
} one used in a Nikon Coolpix 990. The projector lens in the tube of my
} compound microscope is 1.6X.
}
} Thank you for any light anyone can shed on how to do these calculations.
}
Dear Jim,
First, I would calibrate the magnification of the image by taking a
picture of a standard slide, then I'd use the image processing program to
draw a bar on the image file corresponding to a known distance (e.g., 10
micrometers). When the image is printed in the journal--at any size the
journal chooses--that bar will still represent the same distance. If you
want the actual magnification, measure the length of the bar as it appears
on the journal page and divide by the distance. Good luck.
Yours,
Bill Tivol



From daemon Fri Aug 2 03:14:04 2002



From: Jondo Yun À±Á¸µµ :      jdyun-at-kyungnam.ac.kr
Date: Fri, 2 Aug 2002 17:03:35 +0900
Subject: separating thin film and substrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear netters
I am curious if there is a good way of separating submicron thin films from silicon or silica substrate for TEM observation? I guess that hydrfluoric acid would work for most materials even though it has problem of toxicity.

Jondo Yun



From daemon Fri Aug 2 08:17:53 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Fri, 2 Aug 2002 09:01:12 -0400
Subject: Calculating magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim,
Here is what I have experienced, but the best thing would be to test this
using an object of know dimension.

1. The magnification from object to image on the photosensor = M(objective)
x M(projector lens)
2. The magnification of the image from photosensor to paper =
Dimension(paper)/Dimension(sensor)
3. The overall magnifaction is the product of these two =
M(o)xM(p)xD(p)/D(s)

The same formula works for magnification on your computer/video screen.

Everett Ramer
Cellomics, Inc.
Pittsburgh, PA

-----Original Message-----
} From: "JLCastner-at-aol.com"-at-sparc5.microscopy.com
[mailto:"JLCastner-at-aol.com"-at-sparc5.microscopy.com]
Sent: Friday, August 02, 2002 12:26 AM
To: Microscopy-at-sparc5.microscopy.com


Hello All,

Forgive me if my question is elementary, but I am just beginning to so
some simple brightfield photomicroscopy. Is there a simple formula for
calculating the total magnification achieved when a photo is printed? For
example, if you have 10X eyepieces and use a 40X objective when the photo is

exposed, and then print it to a size of 3" X 4" on a journal page, can you
easily calculate the final magnification?

Also, if such formulae do exist, were they developed with the assumption

that one is using a 35mm camera? I am using a Nikon D1X (their top of the
line digital). This camera has a chip, and that chip is different from the
one used in a Nikon Coolpix 990. The projector lens in the tube of my
compound microscope is 1.6X.

Thank you for any light anyone can shed on how to do these calculations.

Sincerely,

Jim Castner


From daemon Fri Aug 2 08:19:35 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Fri, 2 Aug 2002 09:10:38 -0400
Subject: Calculating magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim,
Here is what I have experienced, but the best thing would be to test this
using an object of know dimension.

1. The magnification from object to image on the photosensor = M(objective)
x M(projector lens)
2. The magnification of the image from photosensor to paper =
Dimension(paper)/Dimension(sensor)
3. The overall magnifaction is the product of these two =
M(o)xM(p)xD(p)/D(s)

The same formula works for magnification on your computer/video screen.

Everett Ramer
Cellomics, Inc.
Pittsburgh, PA

-----Original Message-----
} From: "JLCastner-at-aol.com"-at-sparc5.microscopy.com
[mailto:"JLCastner-at-aol.com"-at-sparc5.microscopy.com]
Sent: Friday, August 02, 2002 12:26 AM
To: Microscopy-at-sparc5.microscopy.com


Hello All,

Forgive me if my question is elementary, but I am just beginning to so
some simple brightfield photomicroscopy. Is there a simple formula for
calculating the total magnification achieved when a photo is printed? For
example, if you have 10X eyepieces and use a 40X objective when the photo is

exposed, and then print it to a size of 3" X 4" on a journal page, can you
easily calculate the final magnification?

Also, if such formulae do exist, were they developed with the assumption

that one is using a 35mm camera? I am using a Nikon D1X (their top of the
line digital). This camera has a chip, and that chip is different from the
one used in a Nikon Coolpix 990. The projector lens in the tube of my
compound microscope is 1.6X.

Thank you for any light anyone can shed on how to do these calculations.

Sincerely,

Jim Castner


From daemon Fri Aug 2 08:51:08 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Fri, 02 Aug 2002 09:43:34 -0400
Subject: re:confocal listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listserver,
Could someone post the address of the confocal listserver again?

thanks
Mike D.



From daemon Fri Aug 2 09:17:52 2002



From: Carl Dahlberg :      carl.dahlberg-at-kmf.gu.se
Date: Fri, 2 Aug 2002 16:13:50 +0200
Subject: STM, CCD camera and software for fluorescence recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
We are looking to buy a CCD camera and software for mostly fluorescent
microscopy.
We will need a cooled system and a highly sensitive camera. The main
microscope we will use it on is a Leica MZ FLIII. Any recommendations on
what to buy? As always, are price, performance and relative simplicity of
interest.

Sincerely Carl

Carl.Dahlberg-at-kmf.gu.se



From daemon Fri Aug 2 09:20:14 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Fri, 2 Aug 2002 10:12:26 -0700
Subject: LKB Knifemaker service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TGIF greetings to everyone,
Does Leica provide repair service for the LKB knifemakers?
Are there other service providers out there?
thanks,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Fri Aug 2 09:38:45 2002



From: Wolf Schweitzer :      wuff-at-swisswuff.ch
Date: Fri, 2 Aug 2002 16:35:49 +0200
Subject: Jenoptik CCD Microscanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to alert this forum of this product:

http://www.progres-camera.de/e_progres/content/menuepunkt_2/progresc14_info.htm

We bought one for 10'220 Euro directly off the company (they are an
OEM manufacturer for others, like Leica, but their software support
is maybe worth a look at).
--

Wolf Schweitzer
MD, LEGAL MEDICINE FMH (Switzerland)
Institute of Legal Medicine
Winterthurerstrasse 190
8057 Zuerich, Switzerland
Tel. ++41 1 635 56 22


From daemon Fri Aug 2 09:56:12 2002



From: msteglic-at-mail.mdanderson.org
Date: Fri, 2 Aug 2002 09:51:12 -0500
Subject: Re: Calculating magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




When Bill said "standard slide" I hope he was refering to a stage micrometer.
These can be purchased from most EM suppliers. Once you take a picture at each
objective mag, you can calculate the negative mag. Please note that the
eyepiece plays no part in the negative magnification unless you have an eyepiece
in the image path of the camera. Is you do, it will be included in the
calculated mag from the negative.

Mannie Steglich
Tech Dir. Pathology E M Lab
U T M D Anderson Cancer Center




From daemon Fri Aug 2 10:31:12 2002



From: Tom McKee :      tmckee-at-scilabs.com
Date: Fri, 02 Aug 2002 11:19:48 -0400
Subject: TEM- Turbopumped Vacuum Evaporators - carbon coating applications

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers we could use some advice.

We use carbon support films for TEM preparations which we normally prepare
in diffusion pumped Denton and Ladd evaporators. We have recently added a
turbopumped evaporator from another supplier, but have not been happy with
manufacturer support when we have had problems. We run 24/7 with multiple
users and frequent air/vacuum cycles, sometimes several per hour.

We would appreciate hearing user experience on various models and
suggestions as to our upcoming replacement purchases. Please reply offline
to tmckee-at-scilabs.com or call at 800-476-5227. I will be happy to compile
the responses.

Thanks in advance for your help.

Tom McKee tmckee-at-scilabs.com
Compliance Officer

Scientific Laboratories, Inc. check us out at www.scilabs.com
13635 Genito Rd.
Midlothian, VA 23112
804-763-1200, Fax 804-763-1800





From daemon Fri Aug 2 12:58:29 2002



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 02 Aug 2002 18:06:20 -0400
Subject: Re: EM400T Valve V1 Hiccups

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello folks!

I'm new to the field and still have to get our recently acquired EM400T
working before I can actually try my hand at TEMicroscopy. After months of
preparations, last Tuesday was the big day when I finally threw the giant
main power switch on the wall for the first time and hoped to maniacally
proclaim, "IT'S ALIVE!!" Well, I'm glad I didn't invite
spectators for this one because I didn't get very far, but at least the
EM400 showed a few signs of life.

Once I took care of a nasty hose leak and got the pneumatic pressure up
high
enough, the one symptom that stalled my progress occurs the same way each
time,
always about 20 seconds after powering up the EM400 (rotary pvp running).
Suddenly, valve V1 begins rapidly opening and closing at a pretty regular
pace (somewhat better than once per second and sometimes faster), and this
is also
observable at the V1 LED on the pump system indicator schematic panel on
right side of the lower cabinet.

I've looked at the pullout pcb that's supposed to control V1, and I've
tested the capacitors, but not the other components yet. (The ten micro
farad one didn't test correctly until I took it out of the circuit, but the
numbers looked good on the others while in circuit, so I didn't take them
out.)

Has anyone encountered this problem before or have an idea where I might
look next?
I don't think it is the valve itself unless the LED indicator is designed
to monitor the valve rather than the electronics that control the valve.

Thanks much for any tips!

Best,
-Eric
--
Eric Anderson
SCSU Physics Adjunct
203-392-6455
anderson_e-at-southernct.edu



From root Fri Aug 2 15:32:40 2002
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Just keep your Denton 502, it will last forever!!!!!!!!1
Regards,
Markus F. Meyenhofer
Microscopy Labs
----- Original Message -----
} From: "Tom McKee" {tmckee-at-scilabs.com}
To: "Microscopy List Service" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 02, 2002 11:19 AM


The most direct way is to divide the measured length of the photo image of a
stage micrometer by its actual size. For example, if the photo image is 40
mm wide and the actual distance at the object plane 0.08 mm, then the actual
magnification is X500 (i.e., 40 / 0.08 = 500).

Note that the multiplication sign precedes the magnification number for the
image (e.g., X500), but comes after the magnification number for the
objective (e.g., 40X) by convention.

Gary Gill

-----Original Message-----
} From: Everett Ramer [mailto:eramer-at-cellomics.com]
Sent: Friday, August 02, 2002 8:11 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Jim,
Here is what I have experienced, but the best thing would be to test this
using an object of know dimension.

1. The magnification from object to image on the photosensor = M(objective)
x M(projector lens)
2. The magnification of the image from photosensor to paper =
Dimension(paper)/Dimension(sensor)
3. The overall magnifaction is the product of these two =
M(o)xM(p)xD(p)/D(s)

The same formula works for magnification on your computer/video screen.

Everett Ramer
Cellomics, Inc.
Pittsburgh, PA

-----Original Message-----
} From: "JLCastner-at-aol.com"-at-sparc5.microscopy.com
[mailto:"JLCastner-at-aol.com"-at-sparc5.microscopy.com]
Sent: Friday, August 02, 2002 12:26 AM
To: Microscopy-at-sparc5.microscopy.com


Hello All,

Forgive me if my question is elementary, but I am just beginning to so
some simple brightfield photomicroscopy. Is there a simple formula for
calculating the total magnification achieved when a photo is printed? For
example, if you have 10X eyepieces and use a 40X objective when the photo is

exposed, and then print it to a size of 3" X 4" on a journal page, can you
easily calculate the final magnification?

Also, if such formulae do exist, were they developed with the assumption

that one is using a 35mm camera? I am using a Nikon D1X (their top of the
line digital). This camera has a chip, and that chip is different from the
one used in a Nikon Coolpix 990. The projector lens in the tube of my
compound microscope is 1.6X.

Thank you for any light anyone can shed on how to do these calculations.

Sincerely,

Jim Castner


From root Fri Aug 2 17:02:52 2002
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Message-ID: {009001c23a6b$30c195a0$73d55c0c-at-vitaly}


Hi Carl,

We used http://www.fli-cam.com/ , http://www.sbig.com/ for slow scan only,
check software at http://www.cyanogen.com/index2.html . For slow scan/live
video try http://www.dvcco.com/ . Results were pretty good with all these
cameras. Many others do exist which we didn't try. Contact me off list for
specific information.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Carl Dahlberg {carl.dahlberg-at-kmf.gu.se}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 02, 2002 10:13 AM


Eric,

Is the V1 physically opens? You can see that with the naked eye. If yes,
then proceed further.

First, you have to put a V1 control card (E-U12A) on the extender card, and
check the V1 control signals- there are several, each one easily traceable
to the point of origin- Philips manual has good schematics with the detailed
explanation. You will see at least one signal going through the same cycle
as the valve does. Use fast response meter with hold function, oscilloscope
is even better, as the signal level may change in a matter of milliseconds
back and forth. Very generally, switches and pneumatic components are more
likely to fail as compared with electronic components. A number of time
delays are incorporated into vacuum logic sequences. Problem may be in one
of the delay circuits. You need to trace the faulty signal first.

If I have to guess what's wrong, here it is:
1) Pneumatic safety switch contacts are ringing (replace switch or use
larger capacitor at it's input filter on the E-U13A card, or increase air
pressure within the reasonable limits)
2) Pneumatic solenoid which controls V1 is defective
3) V1 end switch is not engaged, or is defective

Just a guess, could be something other.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Eric Anderson {andersone1-at-southernct.edu}
To: 'microscopy-at-msa.microscopy.com' {microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 02, 2002 1:49 PM


Eric,
I'm not familiar with the EM400T, but I've seen this kind of behavior if
there is a pressure switch for detecting an air pressure failure. If
the pressure is marginal, opening the valve drops the pressure enough to
trip the air pressure failure circuit causing the valve to close. With
no flow on the airline, the pressure recovers and resets the circuit and
allows the valve to open again, dropping the air pressure. Try
increasing the air pressure another 10 psi and see if a) the problem
goes away or b) the frequency of valve operation changes. If nothing
changes, then you probably have an electrical or electronic problem. If
the frequency changes, look for restrictions in your air lines.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Eric Anderson wrote:

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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Fri Aug 2 17:38:08 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Sat, 3 Aug 2002 21:45:55 -0400
Subject: separating thin film and substrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

There are two pneumatic valves located in the pump space, just to the right
of the roughing pump and each of those valves has a small microswitch which
is used to sense whether the valve is open or closed. The problem can
frequently be eliminated by simply adjusting the position of the switch.
This is accomplished by adjusting a small screw which is part of the switch
itself.

Good luck and congratulations on resurrecting an EM-400. It is a fine
instrument.

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone 512/282-5507 FAX 512/280-0702

Sustaining Member - MICROSCOPY SOCIETY OF AMERICA
----- Original Message -----
} From: "Eric Anderson" {andersone1-at-southernct.edu}
To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 02, 2002 12:49 PM


Jondo;

When you say "submicron", how thin do you mean? I uM = 10,000 angstroms.
Is it 100 angstroms or 5,000?

If the film is thick enough, you may be able to fracture a section off
mechanically. I assume you want the film as a sheet of material rather
than in x-section as one might get with a FIB for TEM samples.

You may attempt this chemically if your substrate will dissolve or etch in
something that will not etch or change the characteristics of your film.
You may be able to simply dissolve off the substrate material.

A SERIOUS CAUTION ABOUT USING HF.

Hydrofluoric acid is not just toxic but can cause very serious injury to
human tissue and should only be used by someone that is trained and
experienced in it's handling, disposal and all safety hazards. Please find
out first what precautions to take such as skin and eye protection. HF,
unlike acids such as sulfuric or nitric, will not initially burn and will
simply feel like a skin irritation. You have only minutes to treat the
affected site. In ADVANCE, you should set up with your safety officer a
hospital emergency room that is prepared to treat you if there is an
accident. They need to know the treatment protocol or you may lose a limb
while the medical staff finds out what to do. I say all this from
experience and a serious regard for safety. We use HF routinely for
etching SiN, SiO2 etc.

Peter Tomic
Anadigics, Inc.



-----Original Message-----
} From: Jondo Yun À±Á¸µµ [mailto:jdyun-at-kyungnam.ac.kr]
Sent: Friday, August 02, 2002 4:04 AM
To: MicroscopyListserver


Dear netters
I am curious if there is a good way of separating submicron thin films from
silicon or silica substrate for TEM observation? I guess that hydrfluoric
acid would work for most materials even though it has problem of toxicity.

Jondo Yun



From daemon Sat Aug 3 22:48:20 2002



From: alopes966-at-aol.com (by way of MicroscopyListserver)
Date: Sat, 3 Aug 2002 22:49:35 -0500
Subject: Ask-A-Microscopist: photography in microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (alopes966-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
August 3, 2002 at 18:22:16
---------------------------------------------------------------------------

Email: alopes966-at-aol.com
Name: Alexandre Lopes

Organization: INESP - DivinÛpolis - Brasil

Education: Graduate College

Location: DivinÛpolis - Minas Gerais - Brasil

Question: Oi, one question. How photography in microscopes? I'm
professor of photography in Brasil and i never make this. You have
sugestion for a program of class for biology or wildlife for exemple.
If you have same kind of catalog, brochure or text about your
organization,or simple "old" photomagazines, please send for:

Alexandre Lopes
Rua JosÈ de Alencar, 540
Nova Suissa Belo Horizonte
Minas Gerais Brasil
CEP 30480500

My interess is history, new photo-artists and
contemporanean art photo, ok.
Thank You Very Much


---------------------------------------------------------------------------


From daemon Sat Aug 3 22:48:20 2002



From: ao_reu-at-ccmr.cornell.edu (by way of MicroscopyListserver)
Date: Sat, 3 Aug 2002 22:45:33 -0500
Subject: Ask-A-Microscopist:electropolishing NiTi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ao_reu-at-ccmr.cornell.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
August 3, 2002 at 06:48:45
---------------------------------------------------------------------------

Email: ao_reu-at-ccmr.cornell.edu
Name: Alicia Ortega

Education: Undergraduate College

Location: Ithaca, NY, USA

Question: Hello,
I have been working on the preparation of equiatomic NiTi for EBSD
analysis this summer. As a final polishing step, I have been trying
to electropolish my samples. It seems to do a good job removing the
metal from the sufrace, but sometimes it leaves a build-up of
something on the surface of the samples. I was just wondering what
this build up might be and how I might be able to avaoid this
happening while I'm electropolishing or if there is some way
(non-mechanical) of removing the layer after electropolishing. Any
suggestions would be greatly appreciated as I am quickly running out
of time to work on this.

Thanks again,
Alicia

---------------------------------------------------------------------------


From daemon Sun Aug 4 04:14:47 2002



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Sun, 4 Aug 2002 10:05:06 +0100
Subject: Re: EM400T Valve V1 Hiccups

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

A couple of possible causes:

1. Your compressed air feed is just too low. As the valve moves, the
pressure in the supply drops slightly, causing the microscope to
close the valve. Try turning up the pressure ~0.5 bar.

2. There's a problem with the solenoid-driven valve that is switching
the pneumatic lines. These valves are in a bank under the flat panel
just to the right of the column. The electronics and the solenoid are
probably OK but the pneumatic valve itself may be failing. To start
with, simply switch the valve with another (the acctuating solenoids
are easily slid off), to confirm the problem. On an old EM400T, you
may need to replace all of these valves to get the vacuum system
working reliably.

Regards,
--
Larry Stoter


From daemon Mon Aug 5 04:01:50 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Mon, 5 Aug 2002 10:41:15 +0200
Subject: to subscribe to the CLSM listservier at Buffalo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Send a message including the following:
Sunscribe {Full name}
to: LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU
Here is there webb interface address as well.
http://listserv.acsu.buffalo.edu/cgi-bin/wa

Hope this helps

LISTSERV(R) version 1.8d - most commonly used commands

INFO {topic|listname} Order documentation (plain text files)
SUBscribe listname {full name} Subscribe to a list
SIGNOFF listname Sign off from a list
SIGNOFF * (NETWIDE - from all lists on all servers
Query listname Query your subscription options
Search listname keyword... Search list archives
SET listname options Update your subscription options
INDex {listname} Order a list of LISTSERV files
GET filename filetype Order a file from LISTSERV

There are more commands; send an INFO REFCARD command for a comprehensive
reference card, or just INFO for a list of available documentation files.

If you prefer, you can use LISTSERV through its web interface at
http://listserv.acsu.buffalo.edu/cgi-bin/wa (the full manuals can also be
browsed online at this URL).

This server is managed by:
listmaster-at-listserv.acsu.buffalo.edu


Summary of resource utilization
-------------------------------
CPU time: 0.000 sec
Overhead CPU: 0.010 sec
CPU model: Ultra-2 (384M)



From daemon Mon Aug 5 08:29:50 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 5 Aug 2002 09:19:59 -0400
Subject: RE: confocal listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal (search archives)

http://listserv.acsu.buffalo.edu/cgi-bin/wa?SUBED1=confocal&A=1 (subscribe
or unsubscribe)

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Mike Delannoy
} Sent: Friday, August 2, 2002 9:43 AM
} To: microscopy
} Subject: re:confocal listserver
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear listserver,
} Could someone post the address of the confocal listserver again?
}
} thanks
} Mike D.
}
}
}


From daemon Mon Aug 5 10:29:58 2002



From: msteglic-at-mail.mdanderson.org
Date: Mon, 5 Aug 2002 10:05:40 -0500
Subject: Re: Calculating magnification

Contents Retrieved from Microscopy Listserver Archives
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Jim

I just reread your original post. Let me clarify my previous response. When you
use a stage micrometer it doesn't matter what kind of camera you use. In your
case, use your Coolpix and photograph the scale on the stage micrometer. Make a
print of it the same size you make of anything else photographed using the same
objective lens. Do not crop anything off of either print, print the entire
frame. Pick a distance of say 2mm on the ruler on the print. You can then use a
ruler and using the formula - #mm on the ruler you are measuring with (lets say
50) divided by the # of mm on the print (we said 2). This would give you a print
magnification of x25. If you are using film, you can calculate the negative
magnification for each objective by measuring the ruler on the negative. Then
you would need to calculate the final magnification of the print using the
negative mag times the enlargement magnification.

If I can be of any further help, e-mail me.

Mannie Steglich
U T M D Anderson Cancer center




From daemon Mon Aug 5 12:30:19 2002



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Mon, 5 Aug 2002 13:21:10 -0500
Subject: Hello from MM2002 in Quebec City

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

It is once again that time of year that many of us meet at the
Microscopy and Microanalysis meeting. As in previous
years we will be streaming video from the Computer
workshop area. Feel free to login and virtually join
the meeting even if it is just looking over someones
shoulder. We will move the camera around at different
times during the week.

http://www.msa.microscopy.com

Cheers

Nestor
Your Friendly Neighborhood SysOp


From daemon Mon Aug 5 17:36:37 2002



From: Heather Lock :      dordoztyjq.compwrldmrs-at-menix.bgu.ac.il
Date: Mon, 05 Aug 2002 18:26:05 -1600
Subject: iLinsting your Email ID

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I have been looking for nice man to change emails with and maybe more?

I hoping it you.

http://www.myonlyvalentine.com/?oc=5027








j


From daemon Mon Aug 5 20:15:01 2002



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Tue, 6 Aug 2002 11:09:10 +1000
Subject: HIV in resin - survival?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a student with HIV infected tissue embedded in resin. Does
anyone know whether the HIV survives through all the steps OsO4, UAc,
EtOH, acetone, Epon-Araldite? A library and Web search yielded
nothing useful.

Cheers,

Diana


From daemon Tue Aug 6 08:10:47 2002



From: RUCHIKA781-at-INDIATIMES.COM (by way of MicroscopyListserver)
Date: Tue, 6 Aug 2002 08:49:31 -0500
Subject: Ask-A-Microscopist:LM Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (RUCHIKA781-at-INDIATIMES.COM) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
August 5, 2002 at 23:21:54
---------------------------------------------------------------------------

Email: RUCHIKA781-at-INDIATIMES.COM
Name: RUCHIKA AHUJA

Organization: IRDE DEHRADUN

Education: Graduate College

Location: INDIA DEHRADUN

Question: I am interested to know about a microscope that can provide
500x magnification covering at least 20mm field of view. What should
be its specification?

---------------------------------------------------------------------------


From daemon Tue Aug 6 10:03:27 2002



From: Emmanuelle Roux :      eroux-at-caprion.com
Date: Tue, 6 Aug 2002 10:54:14 -0400
Subject: Re:HIV in Epon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Diana,
Treatment for EPON is very harsh for living tissues. I don't think HIV does survive this kind of treatment. It does'nt even survive freezing (source: an MD ), so imagine with fixative, osmium, ETOH, PO, EPON, heat...
If you are too worried check with a virologist to be 100% sure.
Emmanuelle

Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620



From daemon Tue Aug 6 10:51:54 2002



From: =?ISO-8859-1?Q?G=F6ran_Axelsson?= :      axelsson-at-acc.umu.se
Date: Wed, 07 Aug 2002 00:36:39 +0200
Subject: Moving a Jeol JEM 100CX TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ruchika
no microscope available can cover a
field as large as 20mm in a single image at 500x.
The image would be 1 metre wide!
A field width of 0.25 x 0.20 mm would be nearer the mark -
if the final image magnification is
500 times when enlarged to fit onto 5"x4" film.

Many modern microscopes use digital cameras,
and there are several software
packages that can stitch multiple images together
to cover larger areas than the microscope's limited field width.
However, at 500x you would need to stitch several
thousand images together (at 0.25x0.2mm, 80x100=8000 images),
and the final file size
(1 image = minimum 1Mb: 8000 images =8Gb)would defeat
all but the most powerful computers. Certainly not a
job for the old PC. Well, not mine anyway!

I would put the question back to you and ask why do you need to
cover all of a 20mm specimen at that magnification?
Chris

Date sent: Tue, 6 Aug 2002 08:49:31 -0500
To: microscopy-at-sparc5.microscopy.com
} From: RUCHIKA781-at-INDIATIMES.COM (by way of MicroscopyListserver)


Hello list,

I'm looking for advices on how to move a Jeol JEM 100CX TEM.

I got a Jeol JEM 100CX this spring if I only moved it myself.
After a lot of problems I finally have found a suitable place to put it
in, so the next step is to
move it. I will probably start picking it apart in 1-2 weeks time. The
only problem is that I
have never done it before and I don't have the budget to hire a
technician to do it for me.

I'm doing this as a private hobby project to learn about EM, vacuum
technology and just
for fun. I really hate seeing good old instruments being scrapped. :-)

I'm not all by my self. I have a number of friends that have offered to
help with all from
repairing the electronics (the OL-current control is broken) to actually
help lifting and
driving the truck.

The plan is to use common sense, the mechanical drawings of the column
and a digital
camera to document every step when we take it apart. Then we might have
a fighting chance
to put it back again.
I have talked with the Swedish representant of Jeol but they claimed
they didn't have any
documentation on how to move or put it together. They sounded so
negative so I don't
think I have much help to get there. Maybe they discovered that I wasn't
a customer with a
fat wallet. :-)

As I have learnt a lot from this list by lurking and reading the
archives, (Thanks Nestor!) I now
try to get some advice to how I should move the TEM.
And more importantly, what I should avoid to do!
I'm happy for any advice of horror story you want to share.
If you have some documents to share, I could pay for copying cost and
postage, just let me know.

Whatever the result is I haven't payed much for it, so if I fail to get
it running again I will probably
have learnt a lot and if I get it running I also get a TEM. I just can't
loose! :-)

The diary and details of my EM adventure is documented on
http://www.home.neab.net/gandalf/EM-lab/index.htm
Anyone wanting to see the inside if the OL-current control? Just follow
the link.

If you read this far you are either laughing or just shaking your head.
Thanks anyhow.

Regards from a TEM operator wannabe :-)

Göran Axelsson

Göran Axelsson
Hissjövägen 40
903 45 Umeå
Sweden




From daemon Tue Aug 6 18:22:53 2002



From: Eric Anderson :      andersone1-at-southernct.edu
Date: Tue, 06 Aug 2002 19:16:26 -0400
Subject: Re: EM400T Valve V1 Hiccups

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Vitaly, Ken, Alex, Joel, Larry, & Matt,

Thanks to all for your advice and guidance. Once the pneumatic pressure was
increased from 65 to about 80 psi (5.5 bar) as suggested, V1 stopped opening &
closing. I also found and repaired a wire that had broken away from the
tapered-rod-activated-microswitch on the pneumatic valve down below that's
associated with V1. But now, the rotary pvp (a new Alcotel2012) just shuts off
after only a couple of minutes running (Main display panel then has no PV's
lit.), and after another couple of minutes, the power supply shuts down. (Just
once, the pumping system prematurely switched from PV1 to PV2 for a minute or
so before shutting off.)

This impressive piece of equipment was partly dismantled and in storage for a
few years, plus has experienced a good many miles of bumpy roads and dirt on
the way to its current home. If the budget allows, I think we'll soon have to
hire an experienced EM400 technician to more efficiently carry out what I
expect to be a fair amount of troubleshooting/alignment here. I'm just trying
to solve a few of the simpler problems beforehand, before I get busy with the
fall semester.

Thanks again for all of your help!

Kind regards,
-Eric

Eric Anderson
SCSU Physics Adjunct
203-392-6455
anderson_e-at-southernct.edu

------------------------------------------
Larry Stoter wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello folks!
} }
} } I'm new to the field and still have to get our recently acquired EM400T
} } working before I can actually try my hand at TEMicroscopy. After months of
} } preparations, last Tuesday was the big day when I finally threw the giant
} } main power switch on the wall for the first time and hoped to maniacally
} } proclaim, "IT'S ALIVE!!" Well, I'm glad I didn't invite
} } spectators for this one because I didn't get very far, but at least the
} } EM400 showed a few signs of life.
} }
} } Once I took care of a nasty hose leak and got the pneumatic pressure up
} } high
} } enough, the one symptom that stalled my progress occurs the same way each
} } time,
} } always about 20 seconds after powering up the EM400 (rotary pvp running).
} } Suddenly, valve V1 begins rapidly opening and closing at a pretty regular
} } pace (somewhat better than once per second and sometimes faster), and this
} } is also
} } observable at the V1 LED on the pump system indicator schematic panel on
} } right side of the lower cabinet.
} }
} } I've looked at the pullout pcb that's supposed to control V1, and I've
} } tested the capacitors, but not the other components yet. (The ten micro
} } farad one didn't test correctly until I took it out of the circuit, but the
} } numbers looked good on the others while in circuit, so I didn't take them
} } out.)
} }
} } Has anyone encountered this problem before or have an idea where I might
} } look next?
} } I don't think it is the valve itself unless the LED indicator is designed
} } to monitor the valve rather than the electronics that control the valve.
} }
} } Thanks much for any tips!
} }
} } Best,
} } -Eric
} } --
} } Eric Anderson
} } SCSU Physics Adjunct
} } 203-392-6455
} } anderson_e-at-southernct.edu
}
} A couple of possible causes:
}
} 1. Your compressed air feed is just too low. As the valve moves, the
} pressure in the supply drops slightly, causing the microscope to
} close the valve. Try turning up the pressure ~0.5 bar.
}
} 2. There's a problem with the solenoid-driven valve that is switching
} the pneumatic lines. These valves are in a bank under the flat panel
} just to the right of the column. The electronics and the solenoid are
} probably OK but the pneumatic valve itself may be failing. To start
} with, simply switch the valve with another (the acctuating solenoids
} are easily slid off), to confirm the problem. On an old EM400T, you
} may need to replace all of these valves to get the vacuum system
} working reliably.
}
} Regards,
} --
} Larry Stoter--



From daemon Wed Aug 7 01:19:07 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 06 Aug 2002 23:09:34 -0700
Subject: Re: Moving a Jeol JEM 100CX TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Alesson

I think you are on the right way: use your common sense and everything
should be OK. What I would do in such case:

-turn ON microscope and let air enter the column/gun/camera using regular
cycle.
-turn OFF the microscope (key OFF), unplug from water/electricity/air sources.
-remove the cover to have access to the column;
- you have to disassemble column;
-label all electrical connections - both sides which connected to the
column and cabinets.
-label all vacuum/air/water tubings - both sides - same as before;
-label all parts you are going to disassemble - I would suggest you may
make a few marks for clear indication of the correct orientation. I also
suggest you will attach screws to the place where they were in some small
plastic bag - it's easier than mark all screws! Just in case, mark plastic
bag as well!
- disconnect all electrical cables from the column and from the cabinets,
checking that everything is marked at the both ends!
- similarly disconnect water/air etc.

-Have a lot of heavy-duty alumina foil ready!

-Column, upper part at the level of the objective lens (so the lens would
be in the lover part): detach the vacuum/air etc lines and diffusion pump,
use some wood planks (strong enough) to secure the upper part of the
column: put 4-5 planks around the column and secure them with some strong
metal belts (from Home Depot if you have some?). This construction should
be strong enough to hold the whole weight of the column. You'll use it to
move the column (upper part). Unscrew the bolts holding this part of the
column (between the segments of the column). Then: 5 strong men will
gently move up this part of the column and carefully lay it on the floor
with alumina foil. Use alumina foil to protect the openings in the
column! Check, you don't lost O-ring. Be sure that you do not damage
aperture assembly and valves/connections on the column when moved
it. Cover all openings (even electrical connectors) with alumina foil!!!!!!

- use another sheet of the foil to protect opening on the lower part of the
column/connections etc

- I don't remember exactly, but it seems that 100CX has a central console
with column and two detachable cabinets. You need to try to detach
left/right cabinets (checking for all connections/tubing) from the central
console.

Finally you have to have the following separate parts:

-air compressor;
-mechanicam pump assembly;
-power supply;
-central console with 1/2 column;
-upper part of the column;
-left and right cabinets.

Now, make measurements to insure you may move out all parts. If it's OK -
you are very lucky! This scenario permits to move parts within the
floor. You probably could not use the elevator, it's heavy. You have to
check. If this parts are too big/heavy to move out, your business is
probably hopeless. If you'll disassemble the column on the smaller pieces,
you will need professional help with column's mechanical alignment.

When assemble: clean up all vacuum O-rings (and their sits) with hexane or
95% Ethanol, apply tiny amount of the Apiezone-N on the O-rings (very
little) and assemble in reverse order. When installed upper part of the
column - be sure you orient column correctly and move it very
slow. otherwise you may damage O-ring. Be sure everything is clean and
you do not spoil some dust into the lover part of the column when install
upper part... I am sorry, I am trying to do my the best... I hope it
helps. Good luck. Sergey.

P.S. Some remarks: you have to keep original orientation of the console and
cabinets: do not let leak oil from DP or HV tank. Move vertical and
horizontal only, do not tilt. Be careful with water lines - do not spoil
the water on electronics etc... If you have questions - ask off line if
you wish. I hope you'll enjoy playing with 100CX in the Lego. I like such
games. Russians says: "Who is not risking, that person will not drink
champagne at the end". I wish, you will.

At 03:36 PM 8/6/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Aug 7 06:35:21 2002



From: ROSSCAC-at-aol.com
Date: Wed, 7 Aug 2002 07:24:36 EDT
Subject: SOS:Need references for proper tissue collection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning all at the MSA listserver,
I am looking for reference material that explains proper techniques for
tissue collection and fixation. Currently I am receiveing tissue that is
quite autolytic and I can't seem to find the words to get the idea that
tissue ultrastructure, such as liver and kidney, is extremely vulnerable to
hypoxia (such as when an animal is exsangunated), and delayed fixation. One
concern is that some feel that tissues can be collected at room temperature
in room temperature fixative, especially liver and kidney. In my training,
these particular tissues needed to be collected cold! and placed in cold
fixative to stop metabolism and autolysis. I need referenced help???

Thanks in advance,
Connie

P.S. Hope everyone is having fun at MSA this year!!!!



From daemon Wed Aug 7 09:22:59 2002



From: saram-at-duke.edu
Date: Wed, 07 Aug 2002 10:08:03 -0400
Subject: Re: HIV in resin - survival?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HIV, like cell components, if prepared properly survives just fine in
routine preparations for cell embedment. Many beautiful micrographs
have been published of it. Run a search in PubMed or Medline for
journal articles. It is also pictured in several virology atlases.

Sara Miller




Quoting Diana van Driel {dianavd-at-eye.usyd.edu.au} :

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a student with HIV infected tissue embedded in resin. Does
} anyone know whether the HIV survives through all the steps OsO4, UAc,
}
} EtOH, acetone, Epon-Araldite? A library and Web search yielded
} nothing useful.
}
} Cheers,
}
} Diana
}
}
}


From daemon Wed Aug 7 10:09:09 2002



From: Materials Research Laboratories, Inc. :      mrllab-at-raex.com
Date: Wed, 07 Aug 2002 11:02:26 -0500
Subject: RE: Moving a Jeol JEM 100CX TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Göran Axelsson {axelsson-at-acc.umu.se}

In response to your request for information about moving a JEOL 100CX TEM
there should not be a great deal of difficulty in moving the system or in
getting it up and running. I have moved dozens of SEM and TEM (and other)
instruments and with a little common sense and care the move should actually
be quite easy. A couple months ago I moved a Philips EM100 (about the same
vintage as the JEOL 100CX) and the tear down from running to leaving on the
truck took about 5 hours with two people along with Johnson bars, some 4x4
and 2x4 wood sections and a pallet truck. I hope your move is an adventure
rather than a nightmare.

The first order of business is to make sure that the TEM is shut down
properly (as if for normal servicing) so that the column has been leaked up
to air. If the center console unit will fit through the doors that you need
to maneuver through then you might consider removing the gun assembly at the
top of the column then putting on a blank-off and evacuating the remainder
of the column. You could then move the system under vacuum and make the
restart a much simpler operation. If you are going to need to move the
system up and down stairways rather than using an elevator, then the
tear-down will need to be much more involved and the re-assembly will be a
lot more difficult.

Anyway, once the column has been leaked, you should first remove any and all
decorative or cover panels from the column assembly and the electrical
consoles to make the disassembly process easier. Mark each as to its
location so that it will be simpler when reassembling the system. Next
remove the gun assembly with its high voltage cable. Some of these are oil
filled to prevent electrical arcing so you should take care to maintain the
gun assembly in an upright position so as to avoid oil spillage. Then
remove the other end of the cable from the high voltage tank (which I think
is under the left hand wing console on a JEOL 100CX but am not certain).
Take care not to short the high voltage contacts through this process as
there may be residual high voltage in the tank. Put a plug (usually a screw
cap included on the JEOL tanks) in the high voltage tube on the HT tank. If
there is not a plug attached to the tank then use a rubber cork with some
duct tape to hold it in place.

You will next need to remove the table top to expose the three separate
console units for disassembly and drain any water from cooling lines to
avoid spillage into the column or into the electronics assemblies.

At this point you will need to decide how far to tear down the column to
make the move with the equipment you have available. We usually start by
removing the left side wing console, removing all of the wiring connections
(the harnesses should be kept intact) either from the wing console or from
the column or right wing console (whichever is easier), sliding out the HT
tank and unscrewing the console from the main unit. This should produce a
relatively light weight console with some enclosed electronics, the HT tank
and any panels that needed to be removed in order to get to the bolts
holding things together. Next, remove all of the electrical connections to
the electron beam column and to the main console and bundle these together
with the right hand wing column (again, I may have the location of the HT
tank reversed as to which console it is enclosed in (or it may be separate
altogether but I don't remember that as being the case for the 100CX).
Anyway, then remove the right hand wing console to produce another
relatively light weight section that can be moved. This leaves the pumping
system and the main console that includes the column. If you have the space
for moving this section as a complete unit, that is the easiest way to make
sure the system will be up and running in a relatively short time. If you
need to make the whole thing lighter, start by removing the pumping system
as a unit (if possible) or remove each pumping component separately and make
sure you mark the locations of mating tubing, hoses, electrical connections,
etc. With the pumping system removed you should have left only the main
console, the column and any attached electronics (with the cables either
bundled here or removed and bundled with one or the other of the wing
consoles, whichever was easier). If the weight, height, etc. is still too
great to move this section as is, you will need to remove each column
section one at a time starting at the top, marking the positions of any
electrical or water connections and keeping track of the o-ring that is
associated with each break point (along with bagging and marking the screw
or bolt set that you removed so they don't get confused with any others).
You can remove everything down to the tabletop level if you need to but
this, of course, makes the re-assembly a much more time-consuming process.
This leaves you with the system stripped down about as far as possible
unless you want to remove separately each of the electronics units.

All of the smaller components can usually be moved by one or two people with
four wheeled dollies or simply by carrying. The HT tank may be on its own
wheels. If not it can be elevated onto a four wheel dolly either by lifting
(3-4 people is best) or using a Johnson bar and wood spacers to lift it high
enough to slide under a wheeled dolly. The same is true of the main
console. If you have a small fork lift available the whole assembly can
simply be lifted by sliding the forks under the console being sure that the
weight is distributed well to prevent tipping. You may need to use cross
pieces of wood to make sure of this. If you need to lift the column console
onto a pallet jack, start by lifting either front, back or sides with a
Johnson bar and sliding in a 2x4. Repeat on the opposite side, and then
repeat the process replacing the 2x4 with a 4x4 (or adding another 2x4) as
many times as needed to slide under the wheeled pallet jack or other device
you have available so that the section can be moved. If you are going to
move up and down stairways you will need to strip virtually everything from
the main console so that one or two people can attach the console to a
furniture dolly (or more people can lift the assembly).

In all this, make sure that when you remove or lift something you are not in
danger of breaking water or electrical connections or any other components
that might be bolted onto or protruding from the item you are moving. Take
your time and this should not be a problem. In almost all cases, when we
have broken something on a system it was because we were rushing to get done
instead of taking our time.

Sorry to hear that you didn't get much help from the Swedish JEOL group.
Here in the USA, JEOL service is one of the finest in the world. They have
always been very helpful to me personally and to almost everyone with whom I
have discussed them. As far as a manual, there are some in-house JEOL
documents but they never did issue detailed user manuals for installation as
some of the other EM suppliers have done. They always felt that the
installation was a part of the purchase and sent their engineers to do the
work.

I wish you luck in your TEM moving endeavor. As for your electronics
problem, I unfortunately just scrapped out a good deal of JEOL electronics
from older systems and no longer have any available, just some of the parts
and pieces that we saved. When you collect the system, make sure you also
get all of the manuals that are available for the system in its present
location and, if at all possible, get the JEOL service record log (they
always write a detailed description of their repairs). With the log you
will likely be able to find and diagnose electronics problems and/or vacuum
problems much more quickly since you can look through the records and see if
the same or similar problem was worked on in the past and how it was fixed.

Again, good luck!!

Drew Hirt
President/Senior Scientist
Materials Research Laboratories, Inc.
Struthers, OH USA
drew-at-hirt.com
mrllab-at-raex.com
www.mrllab.com



From daemon Wed Aug 7 10:31:05 2002



From: Qian-Chun Yu, MB, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Wed, 07 Aug 2002 11:23:09 -0400
Subject: Survival of HIV

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

This is certainly an interesting concern for many of us, I thought it might
be worthwhile to mention it on the Network.

I spent more than 3 years doing EM studies of several types of HIV (HIV-1,
HIV-2) and SIV at Harvard AIDS Institute, and we have no evidence that
these lentiviruses can survive the harsh condition used for EM processing.
Based on what we know, HIV is in fact very fragile and does not survive 70%
ethanol and formalin fixation. It is unlikely that they will survive
glutaraldehyde and osmium.

I personally fixed, processed, embedded, and sectioned over a thousand HIV
and SIV-infected cell samples and some autopsies, but never had any
problems with HIV infection. The fact that my colleagues and I are still
alive, healthy, and HIV-negative 10 years after that period is another good
evidence that it is safe to do EM on HIV-infected samples.

Of course one has to be extremely careful about the potential infection
when dealing with these AIDS related samples. All specimens must be
properly fixed inside the hood at a P3 laboratory before being sent out for
EM processing. Even good practice of common sense precautions will help.
However, I do not remember anyone has been able to reinfect cells with HIV
isolated from glut-fixed and epoxy embedded samples.

QC Yu

Qian-Chun Yu, MB, Ph.D.
Director
Cell Imaging Core
Abramson Cancer Research Institute
University of Pennsylvania
421 Curie Boulevard, Room 532
Philadelphia, PA 19104

Phone: 215-573-7766 (Voicemail)
FAX: 215-573-8590
E-mail: qcyu-at-mail.med.upenn.edu



From daemon Wed Aug 7 10:41:15 2002



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Wed, 7 Aug 2002 11:42:12 -0400
Subject: Re: HIV in resin - survival?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Someone needs to define here what is meant by "survives". Are we talking
about preservation of ultrastructure or capacity for infection of
microscopists?

Marie

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
University of Connecticut Unit 2242
Storrs, CT 06269-2242
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Wed Aug 7 11:39:09 2002



From: Materials Research Laboratories, Inc. :      mrllab-at-raex.com
Date: Wed, 07 Aug 2002 12:30:48 -0500
Subject: Re: Moving a Jeol JEM 100CX TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} From: Materials Research Laboratories, Inc. {mrllab-at-raex.com}
} Date: Wed, 07 Aug 2002 11:02:26 -0500
} To: {microscopy-at-sparc5.microscopy.com}
} Subject: RE: Moving a Jeol JEM 100CX TEM
}
} Göran Axelsson {axelsson-at-acc.umu.se}
}
} In response to your request for information about moving a JEOL 100CX TEM
} there should not be a great deal of difficulty in moving the system or in
} getting it up and running. I have moved dozens of SEM and TEM (and other)
} instruments and with a little common sense and care the move should actually
} be quite easy. A couple months ago I moved a Philips EM100 (about the same
} vintage as the JEOL 100CX) and the tear down from running to leaving on the
} truck took about 5 hours with two people along with Johnson bars, some 4x4 and
} 2x4 wood sections and a pallet truck. I hope your move is an adventure rather
} than a nightmare.
}
} The first order of business is to make sure that the TEM is shut down properly
} (as if for normal servicing) so that the column has been leaked up to air. If
} the center console unit will fit through the doors that you need to maneuver
} through then you might consider removing the gun assembly at the top of the
} column then putting on a blank-off and evacuating the remainder of the column.
} You could then move the system under vacuum and make the restart a much
} simpler operation. If you are going to need to move the system up and down
} stairways rather than using an elevator, then the tear-down will need to be
} much more involved and the re-assembly will be a lot more difficult.
}
} Anyway, once the column has been leaked, you should first remove any and all
} decorative or cover panels from the column assembly and the electrical
} consoles to make the disassembly process easier. Mark each as to its location
} so that it will be simpler when reassembling the system. Next remove the gun
} assembly with its high voltage cable. Some of these are oil filled to prevent
} electrical arcing so you should take care to maintain the gun assembly in an
} upright position so as to avoid oil spillage. Then remove the other end of
} the cable from the high voltage tank (which I think is under the left hand
} wing console on a JEOL 100CX but am not certain). Take care not to short the
} high voltage contacts through this process as there may be residual high
} voltage in the tank. Put a plug (usually a screw cap included on the JEOL
} tanks) in the high voltage tube on the HT tank. If there is not a plug
} attached to the tank then use a rubber cork with some duct tape to hold it in
} place.
}
} You will next need to remove the table top to expose the three separate
} console units for disassembly and drain any water from cooling lines to avoid
} spillage into the column or into the electronics assemblies.
}
} At this point you will need to decide how far to tear down the column to make
} the move with the equipment you have available. We usually start by removing
} the left side wing console, removing all of the wiring connections (the
} harnesses should be kept intact) either from the wing console or from the
} column or right wing console (whichever is easier), sliding out the HT tank
} and unscrewing the console from the main unit. This should produce a
} relatively light weight console with some enclosed electronics, the HT tank
} and any panels that needed to be removed in order to get to the bolts holding
} things together. Next, remove all of the electrical connections to the
} electron beam column and to the main console and bundle these together with
} the right hand wing column (again, I may have the location of the HT tank
} reversed as to which console it is enclosed in (or it may be separate
} altogether but I don't remember that as being the case for the 100CX).
} Anyway, then remove the right hand wing console to produce another relatively
} light weight section that can be moved. This leaves the pumping system and
} the main console that includes the column. If you have the space for moving
} this section as a complete unit, that is the easiest way to make sure the
} system will be up and running in a relatively short time. If you need to make
} the whole thing lighter, start by removing the pumping system as a unit (if
} possible) or remove each pumping component separately and make sure you mark
} the locations of mating tubing, hoses, electrical connections, etc. With the
} pumping system removed you should have left only the main console, the column
} and any attached electronics (with the cables either bundled here or removed
} and bundled with one or the other of the wing consoles, whichever was easier).
} If the weight, height, etc. is still too great to move this section as is, you
} will need to remove each column section one at a time starting at the top,
} marking the positions of any electrical or water connections and keeping track
} of the o-ring that is associated with each break point (along with bagging and
} marking the screw or bolt set that you removed so they don't get confused with
} any others). You can remove everything down to the tabletop level if you need
} to but this, of course, makes the re-assembly a much more time-consuming
} process. This leaves you with the system stripped down about as far as
} possible unless you want to remove separately each of the electronics units.
}
} All of the smaller components can usually be moved by one or two people with
} four wheeled dollies or simply by carrying. The HT tank may be on its own
} wheels. If not it can be elevated onto a four wheel dolly either by lifting
} (3-4 people is best) or using a Johnson bar and wood spacers to lift it high
} enough to slide under a wheeled dolly. The same is true of the main console.
} If you have a small fork lift available the whole assembly can simply be
} lifted by sliding the forks under the console being sure that the weight is
} distributed well to prevent tipping. You may need to use cross pieces of wood
} to make sure of this. If you need to lift the column console onto a pallet
} jack, start by lifting either front, back or sides with a Johnson bar and
} sliding in a 2x4. Repeat on the opposite side, and then repeat the process
} replacing the 2x4 with a 4x4 (or adding another 2x4) as many times as needed
} to slide under the wheeled pallet jack or other device you have available so
} that the section can be moved. If you are going to move up and down stairways
} you will need to strip virtually everything from the main console so that one
} or two people can attach the console to a furniture dolly (or more people can
} lift the assembly).
}
} In all this, make sure that when you remove or lift something you are not in
} danger of breaking water or electrical connections or any other components
} that might be bolted onto or protruding from the item you are moving. Take
} your time and this should not be a problem. In almost all cases, when we have
} broken something on a system it was because we were rushing to get done
} instead of taking our time.
}
} Sorry to hear that you didn't get much help from the Swedish JEOL group. Here
} in the USA, JEOL service is one of the finest in the world. They have always
} been very helpful to me personally and to almost everyone with whom I have
} discussed them. As far as a manual, there are some in-house JEOL documents
} but they never did issue detailed user manuals for installation as some of the
} other EM suppliers have done. They always felt that the installation was a
} part of the purchase and sent their engineers to do the work.
}
} I wish you luck in your TEM moving endeavor. As for your electronics problem,
} I unfortunately just scrapped out a good deal of JEOL electronics from older
} systems and no longer have any available, just some of the parts and pieces
} that we saved. When you collect the system, make sure you also get all of the
} manuals that are available for the system in its present location and, if at
} all possible, get the JEOL service record log (they always write a detailed
} description of their repairs). With the log you will likely be able to find
} and diagnose electronics problems and/or vacuum problems much more quickly
} since you can look through the records and see if the same or similar problem
} was worked on in the past and how it was fixed.
}
} Again, good luck!!
}
} Drew Hirt
} President/Senior Scientist
} Materials Research Laboratories, Inc.
} Struthers, OH USA
} drew-at-hirt.com
} mrllab-at-raex.com
} www.mrllab.com



From daemon Wed Aug 7 13:01:24 2002



From: Qian-Chun Yu, MB, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Wed, 07 Aug 2002 13:53:20 -0400
Subject: Definition for "survival"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Excellent suggestion! I took the second "definition" for my response as I
feel "survival" is more related to "life" or viability. For ultrastructure,
we often use "preservation", though sometimes we do use "survive". A
precise definition seems necessary here as we already see two types of
answers. Regards,

QC Yu


Qian-Chun Yu, MB, Ph.D.
Director
Cell Imaging Core
Abramson Cancer Research Institute
University of Pennsylvania
421 Curie Boulevard, Room 532
Philadelphia, PA 19104

Phone: 215-573-7766 (Voicemail)
FAX: 215-573-8590
E-mail: qcyu-at-mail.med.upenn.edu



From daemon Wed Aug 7 13:08:20 2002



From: Barbara Eaglesham :      bse3-at-cornell.edu
Date: Wed, 07 Aug 2002 14:02:14 -0400
Subject: Unsubscribe please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please remove me from the listserver.

Thank you.

Barbara Eaglesham



From daemon Wed Aug 7 14:37:01 2002



From: JHoffpa464-at-aol.com
Date: Wed, 07 Aug 2002 15:28:13 -0400
Subject: Re: SOS:Need references for proper tissue collection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ok first off there is no one proceedure for tissue fixation. when i was doing clinical work we would get kidney tissue sent down on saline soaked gauzr, perfectly find with as much as an hour delay. i have always fixed at room temperature with no real ill effects. there are perhaps 100s of Em books out on the market. if you must articulate then just say what you wrote here.
john


From daemon Wed Aug 7 14:40:52 2002



From: Smartech :      smartech-at-optonline.net
Date: Wed, 07 Aug 2002 15:34:12 -0400
Subject: Hot stage SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a customer that is interested in having the thermal
decomposition of sodium bicarbonate "videotaped", while undergoing SEM at
probably 500X, maybe 1000X. He would be interested in seeing the honeycomb
structure appear from the block-like particles that are seen at RT. (Sodium
bicarbonate decomposes at around 100 deg. C.)

I was thinking a heated stage with either low voltage or low pressure SEM.
Is anyone interested in quoting this project? Sounds interesting.

Ric Felten

SMARTech
860-485-5054
www.semguy.com
19 Cornwall Drive
Goshen CT 06756




From daemon Wed Aug 7 14:43:11 2002



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Wed, 7 Aug 2002 15:20:05 -0400
Subject: LM/EM of Waxes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


August 7, 2002

Good afternoon,
I'd like to hear from anyone that has experience in the microscopy of waxes.
Specifically, I am interested in the means: LM, TEM, other, that have been
successfully used to detect the presence of waxes whether present as a thin
film or sub-micron domains within a host polymer matrix.

Thanks,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com



From daemon Wed Aug 7 16:14:13 2002



From: khara scott :      kharascott-at-yahoo.com
Date: Wed, 7 Aug 2002 14:05:50 -0700 (PDT)
Subject: TEM/SEM - digital processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{TT} Hi everybody out there! {BR} {BR} My department is
planning to possibly get rid of the {BR} darkroom set up
for processing negatives from the TEM {BR} and
SEM. {BR} Does anyone have any suggestions on digital
set ups {BR} that we could go with that would give us
the same {BR} versitility?  Also average
costs? {BR} {BR} {BR} Thanks, {BR} {BR} Khara L.
Scott {BR} Electron Microscopy Technologists
II {BR} Biological Sciences Chicago State
University {BR} Chicago, Il {BR} {BR} {/TT}


__________________________________________________
Do You Yahoo!?
Yahoo! Health - Feel better, live better
http://health.yahoo.com


From daemon Wed Aug 7 17:18:56 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 07 Aug 2002 15:10:25 -0700
Subject: Re: SOS:Need references for proper tissue collection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Connie

It's almost a compromise: to use low or room temperature during the
initial fixation. At higher temperature the fixer works faster (Arrenius?
rule - the speed of chemical reaction will increase twice for each 10oC, so
0-20oC difference gives you x4 increase) - so your tissue supposed to fixed
faster. Again, at higher temperature the fixer penetration is faster as
well. From another hand, these rules applied for every reaction in the
tissue, so the speed of autolisis will increase as well. So, we don't
really know what is the balance between fixation and autolisis.

Personally, I almost perform all procedures for liver/kidney/spleen on
ice. I also used fresh ice-cold EM grade fixer. The time between animal
death and taking sample should be no longer than a few minutes. I usually
prepare everything well ahead: ice box, Petri dish with fixer, sharp new
scalpel, tweezers. We are working in pair: one person taking care of
animal, and me - samples for EM. Working in duet, I am able to take 3x3 mm
piece of liver/spleen or whole kidney in about 40 seconds after animal's
death. I immediately transfer tissue into the Petri Dish with fixer and
cut it on 1x1x1 mm cubes (may be a little bit bigger, you have to do it
quick and not so precise) under the fixer. When all samples had taken
(about 20 min later), I moved samples into the fresh cold fixer and cut
tissue on 0.5x0.5x0.5 cubes. Fixed them for 1-2 more hours on ice. In most
cases I am using 1.5 or 2% GA in 1x PBS (20 mM Na-Phosphate, 150 mM NaCl,
pH 7.4).

This procedure will work for most tissue, but brain. For brain, I am using
room temperature fixer and perfusion. We are working in duet again. We
perfused 1x PBS first (RT) to wash out the blood and fixer is next. After
perfusion I perform all procedures on ice if possible.

To check fixation quality, look on the mitochondria: if crysties is not
bubbled and membranes are parallel, the matrix does not have 'holes' and
uniform - the fixation is OK. Another criteria: nucleolus membrane - if
both membranes are parallel and tight, without big spaces/bubbles between,
nuclear pores are pronounced - it's a good
sign. Expanded/bubbled/non-uniform rER usually is indication of the
hypoxia or some metabolism problems in the cell (not necessary fixation).

I hope it helps. Sergey


At 04:24 AM 8/7/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Aug 7 21:47:17 2002



From: josh :      joshs-at-biotech.ufl.edu (by way of MicroscopyListserver)
Date: Wed, 7 Aug 2002 22:18:23 -0500
Subject: re:hiv in resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I assumed from the phrasing that it was meant the capacity for
infection after fixation. If I'm wrong someone please give me your
opinion on this anyways because I'm curious.
Thanks,
Joshua Steindler


From daemon Thu Aug 8 00:05:35 2002



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Thu, 08 Aug 2002 15:01:50 +1000
Subject: Re: hiv in resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If your tissue has been fixed in glutaraldehyde then you will not have
any infectious material. Glutaraldehyde is the "Agent of Choice" by
hospitals when sterilizing surfaces and instruments after HIV infected
patients have been treated.
Never mind that the staff eventually develop sensitisation to the glut.
Regards
JVN

josh (by way of MicroscopyListserver) wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I assumed from the phrasing that it was meant the capacity for
} infection after fixation. If I'm wrong someone please give me your
} opinion on this anyways because I'm curious.
} Thanks,
} Joshua Steindler
}
}
}


--
John V Nailon
Executive Officer and Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld



From daemon Thu Aug 8 05:28:12 2002



From: Tony Bruton :      Bruton-at-nu.ac.za
Date: Thu, 08 Aug 2002 12:09:40 +0200
Subject: Source of aluminium corrosion in microscope - your views

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Greetings all

An issue to strike terror into the hearts of scanning electron
microscopists !

In the period since our ESEM last received service attention in April
we have had some very nasty crystals develop quite extensively on an
upward facing aluminium surface low down in the column. Unfortunately
the technician who made the discovery did not alert me in time to
photograph the crystal growths - he was spared lynching only by the fact
that he scraped about 5mg of the material into a vial. We have tried
various methods of chemical analysis without anything definitive
emerging, just aluminium and oxygen.

The only clue that we have is that it appears that a specimen
containing high levels of fluoride had been examined several weeks
earlier. I am not an excuse for a chemist but I am informed that
fluorine gas combined with the water vapour semi vacuum of the ESEM
could have been the cause.

Can anyone shed any light on this phenomenon and its possible cause ?

Thanks

Tony

Tony Bruton
University of Natal
Pietermaritzburg
South Africa


From daemon Thu Aug 8 07:42:39 2002



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Thu, 8 Aug 2002 08:34:29 -0400
Subject: TEM position at the US Army Research Laboratory

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







The US Army Research Laboratory has advertised a TEM position. US
CITIZENS ONLY. You may find the posting at:
http://jsearch.usajobs.opm.gov/summary.asp?OPMControl=TS1801. If that page
has expired, I got that information by searching for the announcement #
(ACU201641) at http://www.usajobs.opm.gov/. Here is what I copied from
that site. Please send your applications to the address in the
advertisement and not to me.

Vacancy Announcement

USAJOBS Control No. TS1801 FO

www.USAJOBS.opm.gov, the U. S. Government's official source of job information, provides this
information to the public at no cost.


Announcement No: ACU201641

Opening Date: August 07, 2002

Closing Date: August 20, 2002


Position Title (Pay Plan-Series): MATERIALS RESEARCH ENGINEER (-0806)

Grade: 03

Comments: THIS IS A DELEGATED EXAMINING ANNOUNCEMENT, OPEN TO ALL US CITIZENS.
THIS VACANCY IS NOT COVERED UNDER RESUMIX PROCEDURES. IN ORDER TO BE
CONSIDERED FOR THIS POSITION YOU MUST FOLLOW THE DIRECTIONS UNDER HOW TO APPLY
AND SUBMIT THE PROPER FOR
MS.


YOU MUST SUBMIT A SEPARATE APPLICATION AND ATTACHMENTS FOR EVERY JOB
ANNOUNCEMENT YOU ARE APPLYING FOR. PLEASE MAKE SURE YOUR RESUME/APPLICATION
CONTAINS THE JOB ANNOUNCEMENT NUMBER AND YOUR SOCIAL SECURITY NUMBER. YOU
MUST INCLUDE THE ANNOUNCEMENT NU
MBER ON ALL DOCUMENTS SUBMITTED.


TENURE: Permanent.

NOTES: (1) This is a career-conditional appointment. Career and
career-conditional employees selected under this announcement will be required
to serve an initial probationary period of up to three years. (2) You must
follow the procedure stated in th
e "How to Apply" section to receive consideration under this announcement.
(3) Employee will be required to obtain and maintain a Secret security
clearance. (4) Incumbent will be required to submit an annual Financial
Disclosure statement.


The U.S. Army Research Laboratory is participating in an alternative personnel
system known as the Personnel Demonstration Project. Among other features,
the Demonstration Project replaced GS grade levels with occupational families
and paybands. The s
alary range of the position being filled will not be decreased with the change
to paybands.



FILING DEADLINE: APPLICATIONS MUST BE RECEIVED BY THE CLOSING DATE. LATE
APPLICATIONS WILL NOT BE CONSIDERED.
Number of vacancies to be filled by this announcement : One (1).


Salary: $55,694

Region: Northeast

Organization: US Army Research Laboratory
Sensors and Electron Devices Directorate
Radio Frequency and Electronics Division


Duty Station: Adelphi, MD

Area of Consideration:
Opened to all applicants with or without Civil Service Status.

Duties: Work involves research into improved microanalysis techniques for
electronic devices and microanalysis of new device types. Expertise,
knowledge, and skills include materials science, semiconductor device
technology and sensitive analytical tec
hniques as well as related areas such as semiconductor processing technology
and device physics.

Serves as an engineer responsible for carrying out advanced research and
development activities involving complex equipment, emerging technologies or
scientific phenomena. The work involves research, development or systems
analysis of new equipment, mat
erial, or concepts that significantly add to the understanding and usefulness
of previously unexplained or untested phenomena or contribute to the solution
of significant Army problems. Is considered to be a productive professional,
providing technical
advice and guidance to managers, supervisors, peers, and sponsors on various
aspects of the work. In many instances, experimental data are nonexistent or
controversial requiring the incumbent to develop interpretations and
procedures to extend existing
knowledge/methodology. Is responsible for technically defending and supporting
ideas and proposals for concepts that are often controversial or novel.
Technical contributions are recognized by management and peers as having
signific!
ant impact on ongoing projects and reflect originality and creativity. Attends
and presents papers at conferences or professional society meetings, serves on
technical committees within the agency, and coordinates with other
professionals when working o
n collaborative efforts. Incumbent is skilled in applying a range of
scientific principals, techniques and methods in a specialty area.
Investigates problems of considerable complexity and finds non-obvious
solutions. Results of work make a considerable
contribution in resolving Army problems; advance scientific knowledge and
understanding or capability; or overcome technical obstacles recognized by
other professionals as highly complex. Conceives and formulates ideas or
produces work of such original
ity, soundness and value as to have marked the incumbent as a significant
contributor to the field. Guides and evaluates the design and development
activities of contractors and others in achieving new products. Uses complex
theoret!
ical, experimental and investigative techniques to resolve bot

Performs other duties as assigned.

Qualification Requirements: Basic Education Requirement for Engineers:
A. Candidates must show successful completion of a full four-year
professional engineering curriculum leading to a bachelors or higher degree in
an accredited college or university. Acceptable curriculums must be
accredited by the Accreditation Board
for Engineering and Technology (ABET) or include mathematics and engineering
science or physics courses in the required areas.
OR
B. Candidates may substitute for the above basic requirement at least four
years of college-level education, training or technical experience that
furnished a knowledge and understanding of engineering science and techniques
equivalent to that provided
by a full four-year professional engineering curriculum. (The adequacy of
such background must be demonstrated by (1) professional registration, (2)
written test (EIT) examination), (3) specific academic courses or (4) related
curriculum).

NOTE:
Foreign Education: Foreign education must be evaluated for U.S. equivalency
in order to be rated eligible for this position. Please include this
information either in your resume or by furnishing a copy of your certificate
in your application package.




In addition to the Basic Requirements stated above, applicant must possess one
year of specialized experience equivalent to the GS-11 grade level in the
federal service.

SPECIALIZED EXPERIENCE: Specialized experience is experience which has
equipped the applicant with the knowledge, skills and abilities (KSAs)
necessary to successfully perform the duties of the position and is typically
in or related to materials scienc
e, semiconductor device technology and sensitive analytical techniques as well
as related areas such as semiconductor processing technology and device
physics.

Selective Placement Factors/Knowledge Skills and Abilities (KSA's):
KNOWLEDGE, SKILLS AND ABILITIES (KSAs): Candidates will be rated on their
possession of the following knowledge, skills, and abilities. Candidates must
address each of the KSAs specifi
cally on plain bond paper and submit it along with the other application
materials. Information may include experience, education, training and awards
as it relates to each KSA. Since you will be rated based on your possession
of the KSAs listed in thi
s announcement and a ranking determination made which affects your chances for
employment, it would benefit you to provide your responses to the KSAs on a
separate sheet of paper and submit it with your application.


KSA 1. Ability to perform research and development in electronic material
characterization, growth and device characterization with direct working
knowledge of Transmission Electron Microscopy (TEM), X-Ray Diffraction and
Metal Organic Chemical Vapor D
eposition (MOCVD) tools and processes.

KSA 2. Ability to perform research and development in electronic material
characterization, growth and device characterization with general knowledge of
other analysis techniques such as Scanning Electron Microscopy (SEM),
Secondary Ion Mass Spectromet
ry (SIMS), Auger Electron Spectrometry (AES), etc.

KSA 3. Ability to communicate in writing.

Standard/Other Requirements:
1. Failure to provide all of the required information as stated in the
announcement may result in an ineligible rating or may affect the overall
rating.
2. Incumbent is required to file an annual financial statement.
3. Permanent change of station (PCS) funds will be authorized.
4. Selection for this position is contingent upon proof of U.S. citizenship.
5. Direct Deposit is REQUIRED : As a condition of employment, candidates
appointed, competitively promoted or reassigned are required to enroll and
participate in Direct Deposit/Electronic Funds Transfer within 60 days
following the effective date of t
hat action.
6. Application/Resume deadline: Application/Resume must be received by the
Closing Date of the Vacancy Announcement.
7. Male applicants born after December 31, 1959, are required to complete a
Pre-Employment Certification Statement for Selective Service registration
prior to appointment. Failure to comply may be grounds for withdrawal of an
offer of employment, or di
smissal after appointment.
8. HOW TO APPLY:
Submit the following documents (Numbers 1-4) to the address listed under Where
To Submit Package:

1. OF612, Optional Application for Federal Employment (this form can be found
at www.opm.gov/forms/word/of612.doc, or a Resume. The resume may be typed or
legibly handwritten and must contain, at a minimum: Announcement Number; Name;
Address; Social Sec
urity Number; Position Title and Grade of the job you are applying for; your
paid/unpaid work experience including job title, duties and accomplishments,
employers name and address, supervisors name and phone number, starting and
ending dates (Month and
Year), hours worked per week and grade/salary; education.

2. Separate sheet(s) of bond paper describing how your experience, education,
training, awards relate to the Knowledge, Skills, and Abilities (KSAs) listed
in this announcement. Since you will be rated based on your possession of the
KSAs listed in this
announcement and a ranking determination made which affects your chances for
employment, it would benefit you to submit your responses to the KSAs along
with your application. Since failure to do so would result in the examiner
having less pertinent j
ob-related information in which to evaluate you, a lower rating could result.

3. College Transcripts. NOTE: IF EDUCATION IS BEING USED IN LIEU OF
EXPERIENCE, A COPY OF YOUR TRANSCRIPTS MUST BE PROVIDED. (If you are a
current Federal employee holding a position requiring the same basic
qualifications as the position for which
you are applying, a Notification of Personnel Action (SF-50) will be accepted
in lieu of transcripts.)

4. Applicants claiming veterans' preference must CLEARLY do so in their
resume/application. Applicants claiming 5-point preference must include
specific, detailed information in their resume/application which supports
their claim for veterans' prefere
nce, e.g., actual dates of service, type of duty (active, reservist), campaign
badges or medals awarded, rank at time of retirement, etc. If information
needed to verify entitlement to veterans preference is not provided in the
resume/application, prefe
rence will be denied. Applicants claiming 10-point preference MUST submit a
DD Form 214 AND supporting documentation, e.g., Letter from VA dated within
one year. Failure to submit supporting documentation will result in loss of
consideration for 10-po
int preference. If veterans preference is awarded and the applicant selected,
a DD Form 214 (Member-4 copy) is required at the time of appointment to verify
preference. Failure to provide the DD Form 214 at the time of appointment!
will result in the offer of employment being withdrawn.


NOTE FOR MILITARY SPOUSES: Spouse preference eligibles must provide a copy of
sponsors Permanent Change of Station (PCS) orders AND clearly state in their
resume that they are requesting Military Spouse Preference.


SPECIAL PRIORITY CONSIDERATION UNDER THE INTERAGENCY CAREER TRANSITION
ASSISTANCE PLAN (ICTAP). If you are a displaced Federal employee (Non-DOD),
you may be entitled to receive special priority consideration under ICTAP. If
you are a displaced Departm
ent of Defense (DOD) employee, DOD has established other programs such as the
Priority Placement Program (PPP), and Reemployment Priority List (RPL) for
DODs displaced employees. If you have questions, contact your appropriate
Civilian Assistance and Re
employment Program (CARE) office. For ICTAP, (NOTE: Eligibility expires (a)
one year after separation; (b) one year after an agency certifies that an
employees compensation (OWCP) has been terminated and the individual can not
be placed at the agency;
(c) one year after an employees disability annuity has been terminated or
after being notified that his/her annuity will be terminated; (d) when an
employee accepts a position without time limitations; (e) when an employee no
longer!
meets eligibility requirements; or (f) within a specific agency, upon
declination of offer to that employee by that agency.)

To receive consideration under ICTAP (Numbers 1-7 below), you must:
1. Be a current or former career or career-conditional (Tenure group I or II)
competitive service employee who has been displaced.

2. Be applying for a position at or below the grade level of the position from
which you have been separated. The position must not have a greater promotion
potential than the position from which you were separated.

3. Have a current (or last) performance rating of record that is fully
successful or better. This must be submitted with your application package.
(This requirement does not apply to candidates who are eligible due to
compensable injury or disability re
tirement.)

4. Occupy or be displaced from a position in the same local commuting area of
the position for which you are requesting priority consideration.

5. Have your application received (unless otherwise specified by the
announcement) by the closing date and meet all of the application criteria
(e.g., submit all required documentation, etc).

6. Submit a copy of the appropriate documentation with your application
package, such as a RIF separation notice, a letter from OPM or your agency
documenting your priority consideration rights.

7. Be rated well-qualified. A well qualified employee is defined as meeting
all of the minimum qualification standards and eligibility requirements as
well as possessing knowledge, skills and abilities that clearly exceed the
minimum qualification requ
irements for the position. To be rated well qualified, ICTAP applicants must
attain an eligibility rating on this case examination of 80 points or higher,
not including points for veterans preference.

NOTE: If you wish to be considered through this program, please mark (ICTAP)
clearly on your application.


Where to Submit Application Package:

Please send all required application materials to:
Northeast CPOC
314 Johnson Street
Attention: DEU
Aberdeen Proving Ground, MD 21005-5283

You may fax your complete application package to 410-306-1284 or DSN 458-1284,
ATTN: DEU.


NOTE: In order to receive consideration, your application must contain all of
the applicable information/documents listed under How To Apply. Applications
received through the use of postage paid government envelopes are in violation
of 18 USC 1719 and
will not be considered.
THE DEPARTMENT OF THE ARMY IS AN EQUAL OPPORTUNITY EMPLOYER.
All qualified applicants will receive appropriate consideration
without regard to non-merit factors such as race, color,
religion, sex, national origin, marital status except where
specifically authorized by law, age, politics, disability,
or sexual orientation which do not relate to successful
performance of the duties of this position. Reasonable
accommodation to individuals with disabilities will be provided
upon request.

SELECTION FOR THIS POSITION IS SUBJECT TO RESTRICTIONS
RESULTING FROM DEPARTMENT OF DEFENSE REFERRAL SYSTEM FOR
DISPLACED EMPLOYEES.
Army Civilian Personnel Online (CPOL)






Sincerely,
Matthew Ervin, Ph.D.
(301)394-0017 phone, (301)394-1559 fax
MErvin-at-ARL.Army.mil

M/S: AMSRL-SE-RL
US Army Research Laboratory
2800 Powder Mill Road
Adelphi, MD 20783-1197

Disclaimer: The opinions and views expressed above are those of the author
and do not necessarily represent those of the U.S. Army Research Laboratory
or any other government agency





From daemon Thu Aug 8 10:14:37 2002



From: =?ISO-8859-1?Q?G=F6ran_Axelsson?= :      axelsson-at-acc.umu.se
Date: Thu, 08 Aug 2002 17:22:08 +0200
Subject: Re: Moving a Jeol JEM 100CX TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tony,

Without going into a long "dissertation" like others, it sounds like the
standard corrosion we get from any aluminum parts that are near an ocean.

Many of my customers are less than 10 miles from the beach & I notice
corrosion problems with relays, connectors as well as panel parts.
Other environmental problems have been with the deterioration of rubber
hoses in the LA area (years ago). I am informed that the smog causes the
rubber to crack.

Hope this helps,

Earl


----- Original Message -----
} From: "Tony Bruton" {Bruton-at-nu.ac.za}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, August 08, 2002 3:09 AM


Thanks to all that answered on and off the list!

The amount of advices that I got was far more than I ever hoped for.
Armed with all these advices
and instructions I feel confident that the move will go without problems.

I also am sorry that I wrote the part about the Swedish JEOL office, I
have only got positive
comments about the service you get from JEOL, so I will give it a second
try to see if they
have any advices.
Maybe I just got the wrong guy on the phone or I call at a bad time.
One occasion isn't enough for forming an opinion and I should be old
enough to know that.
Anyhow, I have to give them credit to supporting this instrument for the
last 20 years, because
it's in perfect condition as far as I could see.

I will report back to the list when I have installed it and put it all
together.

Regards, Göran Axelsson.




From daemon Thu Aug 8 12:32:45 2002



From: DrJohnRuss-at-aol.com
Date: Thu, 8 Aug 2002 13:23:44 EDT
Subject: Image Analysis Short Cours

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The three-day intensive hands-on workshop on Image Processing and Measurement
presented by John Russ (author of "The Image Processing Handbook" and
"Practical Stereology") through the North Carolina State University
Department of Continuing and Professional Education is now in its 21st year.
The upcoming course dates are November 6-8, 2002, and May 21-23, 2003, in
Raleigh, and June 9-11, 2003, at the Danish Technological Institute in
Taastrup, Denmark (near Copenhagen). This course has generated highly
favorable reviews from the thousands of previous students. The primary focus
is on images from various types of microscopy, with practical guidance in
correcting imaging defects, enhancing the images for presentation and
measurement, and performing stereological meaningful measurements on them.
Textbooks and computer software are provided to attendees. Lab sessions with
an opportunity to bring your own images makes this course immediately useful
and highly productive.

For full information on the course, including outlines, faculty information,
a downloadable brochure, and on-line registration, go to

{http://members.aol.com/ipcourse}

Class size is limited to maintain a high ratio of instructors to students, so
make your reservation now. You may also contact Cindy Allen at NCSU
Continuing Education, at 919-515-8171


From daemon Thu Aug 8 13:29:43 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 08 Aug 2002 11:20:33 -0400
Subject: Re: Ask-A-Microscopist:LM Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 8/6/02 12:44 PM, Chris Jeffree at cjeffree-at-srv0.bio.ed.ac.uk wrote:

Dear Ruchika and Chris,

} no microscope available can cover a
} field as large as 20mm in a single image at 500x.
} The image would be 1 metre wide!

Worse! The image would be 10 m wide.

} A field width of 0.25 x 0.20 mm would be nearer the mark -
} if the final image magnification is
} 500 times when enlarged to fit onto 5"x4" film.
}
} Many modern microscopes use digital cameras,
} and there are several software
} packages that can stitch multiple images together
} to cover larger areas than the microscope's limited field width.
} However, at 500x you would need to stitch several
} thousand images together (at 0.25x0.2mm, 80x100=8000 images),
} and the final file size
} (1 image = minimum 1Mb: 8000 images =8Gb)would defeat
} all but the most powerful computers. Certainly not a
} job for the old PC. Well, not mine anyway!
}
In addition, someone would have to check the alignment to make sure that
the images had been stitched together properly, since, at the rate of 1
image per second, it will take over 2 hrs just to collect the images, and
temperature variations over the 20 mm field during that time can cause an
automated stitching program to give poor results.
Yours,
Bill Tivol



From daemon Thu Aug 8 16:03:55 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 8 Aug 2002 15:53:48 -0500
Subject: Source of aluminium corrosion in microscope - your views please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tony,

If you have a light element EDS detector, you should be able to see fluorine
if present in the company of aluminum. F should be detectable if it reacted
with the Al (unless that compound is not stable in its environment and the F
was lost as a gas in a continuing reaction of some sort???). Going on
memory (did not look-up), aluminum fluoride is rather reactive itself.
Maybe a chemist can expound on that...

When aluminum reacts in an aqueous environment it typically forms a white
powder (aluminum oxide/hydroxide).

This would be a highly unusual situation for a high vacuum system, but not
so surprising for a variable pressure system using water vapor.

Woody White
McDermott Technology Inc.

http://woody.white.home.att.net


-----Original Message-----
} From: Tony Bruton [mailto:Bruton-at-nu.ac.za]
Sent: Thursday, August 08, 2002 6:10 AM
To: Microscopy-at-sparc5.microscopy.com



Greetings all

An issue to strike terror into the hearts of scanning electron
microscopists !

In the period since our ESEM last received service attention in April
we have had some very nasty crystals develop quite extensively on an
upward facing aluminium surface low down in the column. Unfortunately
the technician who made the discovery did not alert me in time to
photograph the crystal growths - he was spared lynching only by the fact
that he scraped about 5mg of the material into a vial. We have tried
various methods of chemical analysis without anything definitive
emerging, just aluminium and oxygen.

The only clue that we have is that it appears that a specimen
containing high levels of fluoride had been examined several weeks
earlier. I am not an excuse for a chemist but I am informed that
fluorine gas combined with the water vapour semi vacuum of the ESEM
could have been the cause.

Can anyone shed any light on this phenomenon and its possible cause ?

Thanks

Tony

Tony Bruton
University of Natal
Pietermaritzburg
South Africa


From daemon Thu Aug 8 16:10:02 2002



From: Delilah Wood :      wood-at-pw.usda.gov
Date: Thu, 08 Aug 2002 14:01:03 -0700
Subject: Unsubscribe (was Re: )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 02:01 PM 8/8/2002, you wrote:

} Delilah F. Wood, Botanist
} Microscopy & Imaging Lab
} U. S. Department of Agriculture
} Agricultural Research Service
} Western Regional Research Center
} 800 Buchanan St.
} Albany, CA 94710
}
} Phone: 510-559-5653
} Fax: 510-559-5818
} Email: wood-at-pw.usda.gov
} Web site: http://www.pw.usda.gov
Unsubscribe Microscopy "wood-at-pw.usda.gov"



From daemon Thu Aug 8 16:10:29 2002



From: Delilah Wood :      wood-at-pw.usda.gov
Date: Thu, 08 Aug 2002 14:04:47 -0700
Subject: Unsubscribe (was Re: )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 02:04 PM 8/8/2002, you wrote:

} Delilah F. Wood, Botanist
} Microscopy & Imaging Lab
} U. S. Department of Agriculture
} Agricultural Research Service
} Western Regional Research Center
} 800 Buchanan St.
} Albany, CA 94710
}
} Phone: 510-559-5653
} Fax: 510-559-5818
} Email: wood-at-pw.usda.gov
} Web site: http://www.pw.usda.gov
Unsubscribe Microscopy "wood-at-pw.usda.gov"



From daemon Thu Aug 8 16:58:29 2002



From: Qian-Chun Yu, MB, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Thu, 08 Aug 2002 17:51:50 -0400
Subject: Re: hiv in resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I certainly agree with you. Although HIV can develop drug resistance, it is
not that omni-resistant. As I mentioned in the other note, we raised that
issue at Harvard way back in early 1989 and did a primitive test of several
common disinfectant agents. Most of them, including aldehyde fixative and
ethanol, can effectively kill these viruses. The test was never published
since we thought it was just a simple "observation" and was only intended
for better lab practice. But it is important that we should remind
ourselves about the potential risk. Regards,

QCY

At 03:01 PM 8/8/2002 +1000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Qian-Chun Yu, MB, Ph.D.
Director
Cell Imaging Core
Abramson Cancer Research Institute
University of Pennsylvania
421 Curie Boulevard, Room 532
Philadelphia, PA 19104

Phone: 215-573-7766 (Voicemail)
FAX: 215-573-8590
E-mail: qcyu-at-mail.med.upenn.edu



From daemon Thu Aug 8 18:04:29 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 8 Aug 2002 23:59:27 +0100
Subject: Re: Ask-A-Microscopist:LM Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill
You're right, of course - my abacus needs servicing!
Chris

} Worse! The image would be 10 m wide.
}
} } A field width of 0.25 x 0.20 mm would be nearer the mark -
} } if the final image magnification is
} } 500 times when enlarged to fit onto 5"x4" film.
} }
} } Many modern microscopes use digital cameras,
} } and there are several software
} } packages that can stitch multiple images together
} } to cover larger areas than the microscope's limited field width.
} } However, at 500x you would need to stitch several
} } thousand images together (at 0.25x0.2mm, 80x100=8000 images),
} } and the final file size
} } (1 image = minimum 1Mb: 8000 images =8Gb)would defeat
} } all but the most powerful computers. Certainly not a
} } job for the old PC. Well, not mine anyway!
} }
} In addition, someone would have to check the alignment to make
sure that
} the images had been stitched together properly, since, at the rate
of 1
} image per second, it will take over 2 hrs just to collect the
images, and
} temperature variations over the 20 mm field during that time can
cause an
} automated stitching program to give poor results.
} Yours,
} Bill Tivol
}
}



From daemon Thu Aug 8 21:13:09 2002



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 9 Aug 2002 13:24:18 +1000
Subject: Re: HIV in resin - survival?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl & Co.;

Since we are on the topic of corrosion & EM, I would like to know if anyone
near a body of salt water has noticed pitting on front surface coated
mirrors in "optical" microscopes. To be specific, the type that uses
visible light and the light is detected by the electromagnetic sensors
symmetrically spaced about our noses.

I've never been in a lab. that was so close to an ocean that I can attribute
any corrosion I've ever seen from salt air but I'd like to hear from anyone
using optical tables [lasers/mirrors] or optical microscopes that knows this
is a problem, and if shown to be, what is the solution? The only issue I
have ever had with front surface coated mirror/optics is human fingerprints
etching the surface or wet chemistry vapors in a fume hood doing the same.

Regards,

Peter Tomic
Anadigics, Inc.


-----Original Message-----
} From: Earl Weltmer [mailto:earlw-at-sbcglobal.net]
Sent: Thursday, August 08, 2002 10:59 AM
To: Tony Bruton; Microscopy-at-sparc5.microscopy.com


Hi Tony,

Without going into a long "dissertation" like others, it sounds like the
standard corrosion we get from any aluminum parts that are near an ocean.

Many of my customers are less than 10 miles from the beach & I notice
corrosion problems with relays, connectors as well as panel parts.
Other environmental problems have been with the deterioration of rubber
hoses in the LA area (years ago). I am informed that the smog causes the
rubber to crack.

Hope this helps,

Earl


----- Original Message -----
} From: "Tony Bruton" {Bruton-at-nu.ac.za}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, August 08, 2002 3:09 AM


Thank you to everyone who replied - many privately. I did mean
capacity for infection, not ultrastructure. All who replied stated
that infection was impossible, HIV being quite a fragile beastie.

Cheers,

Diana

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Thu Aug 8 22:56:55 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Thu, 08 Aug 2002 23:58:58 -0400
Subject: Re: Source of aluminium corrosion in microscope - your views

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tony,

You should attempt to excavate some material from the corrosion pit that
undoubtedly resides at the point of attachment of one of these crystals. A
fine tungsten needle works well for the job. Analyze what you extract and
look for halogens and transition metals.

A likely cause is that fine particles of some transition metal halide landed
on the aluminum and established a galvanic cell whereby the metal was
reduced and aluminum halide was produced as a transitory product.
Conversion of the aluminum halide to aluminum hydroxide liberates the
halogen so that it re-enters the catalytic cycle of attack on the aluminum.

The seemingly stable silver chloride will provide a dramatic demonstration
of this. A flake of silver chloride in contact with aluminum, even heavily
anodized aluminum, will often result in run-away pitting such that the next
morning a puddle of deliquescent aluminum chloride mixed with aluminum
hydroxide "curd" remains where the chip was. In the high vacuum environment
of the SEM the process would be slower and more likely result in a solid
product.

A very small amount of chloride may be responsible for a much larger volume
of corrosion because it is not consumed in the final products.

John Twilley
Conservation Scientist

Tony Bruton wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Greetings all
}
} An issue to strike terror into the hearts of scanning electron
} microscopists !
}
} In the period since our ESEM last received service attention in April
} we have had some very nasty crystals develop quite extensively on an
} upward facing aluminium surface low down in the column. Unfortunately
} the technician who made the discovery did not alert me in time to
} photograph the crystal growths - he was spared lynching only by the fact
} that he scraped about 5mg of the material into a vial. We have tried
} various methods of chemical analysis without anything definitive
} emerging, just aluminium and oxygen.
}
} The only clue that we have is that it appears that a specimen
} containing high levels of fluoride had been examined several weeks
} earlier. I am not an excuse for a chemist but I am informed that
} fluorine gas combined with the water vapour semi vacuum of the ESEM
} could have been the cause.
}
} Can anyone shed any light on this phenomenon and its possible cause ?
}
} Thanks
}
} Tony
}
} Tony Bruton
} University of Natal
} Pietermaritzburg
} South Africa





From daemon Fri Aug 9 00:21:42 2002



From: IMGENEX :      cservice_imgenex-at-netzero.net
Date: Fri, 9 Aug 2002 10:47:18 +0500
Subject: siRNA Kits Now Available From IMGENEX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



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From daemon Fri Aug 9 07:47:39 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Fri, 9 Aug 2002 10:07:12 -0230
Subject: flatbed scanning with polarized light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For a multi-faceted project involving many researchers, I wanted to
document a polished sulfide surface for spacial notations and sharing ... I
thought the least I could do is scan the surface with a flatbed. As those
who may have tried this know, the result is very dark because the lamp &
sensor are not in close enough angular alignment. This was a
disappointment, because I wanted to try modifying the scanner with a
polarized sheet of mylar.

My question for the list: Do I rule out ALL flatbed scanners for this?
Has anyone encountered a scanner for which the alignment is close enough for
measuring mirror-like (and anisotropic) relectances??

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From daemon Fri Aug 9 10:03:59 2002



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Fri, 9 Aug 2002 10:48:49 -0400
Subject: Moving a Jeol JEM 100CX TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Göran,

The responses from Sergey and Drew are pretty good and complete- follow them
and you
will be fine.

What exactly is broken in the OL current control? Is that one of the
focusing switches/potentiometers at the front panel, or an OL control
circuit itself? Please clarify, maybe we can find a replacement, or suggest
a substitute.

It is surprising to hear that JEOL is not helpful. They are very good here
in USA. Try again- perhaps they were just overworked at the moment...

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Göran Axelsson {axelsson-at-acc.umu.se}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, August 06, 2002 6:36 PM


Hello list,

I'm looking for advices on how to move a Jeol JEM 100CX TEM.

I got a Jeol JEM 100CX this spring if I only moved it myself.
After a lot of problems I finally have found a suitable place to put it
in, so the next step is to
move it. I will probably start picking it apart in 1-2 weeks time. The
only problem is that I
have never done it before and I don't have the budget to hire a
technician to do it for me.

I'm doing this as a private hobby project to learn about EM, vacuum
technology and just
for fun. I really hate seeing good old instruments being scrapped. :-)

I'm not all by my self. I have a number of friends that have offered to
help with all from
repairing the electronics (the OL-current control is broken) to actually
help lifting and
driving the truck.

The plan is to use common sense, the mechanical drawings of the column
and a digital
camera to document every step when we take it apart. Then we might have
a fighting chance
to put it back again.
I have talked with the Swedish representant of Jeol but they claimed
they didn't have any
documentation on how to move or put it together. They sounded so
negative so I don't
think I have much help to get there. Maybe they discovered that I wasn't
a customer with a
fat wallet. :-)

As I have learnt a lot from this list by lurking and reading the
archives, (Thanks Nestor!) I now
try to get some advice to how I should move the TEM.
And more importantly, what I should avoid to do!
I'm happy for any advice of horror story you want to share.
If you have some documents to share, I could pay for copying cost and
postage, just let me know.

Whatever the result is I haven't payed much for it, so if I fail to get
it running again I will probably
have learnt a lot and if I get it running I also get a TEM. I just can't
loose! :-)

The diary and details of my EM adventure is documented on
http://www.home.neab.net/gandalf/EM-lab/index.htm
Anyone wanting to see the inside if the OL-current control? Just follow
the link.

If you read this far you are either laughing or just shaking your head.
Thanks anyhow.

Regards from a TEM operator wannabe :-)

Göran Axelsson

Göran Axelsson
Hissjövägen 40
903 45 Umeå
Sweden







From daemon Fri Aug 9 10:33:56 2002



From: Sara Miller :      saram-at-duke.edu
Date: Fri, 9 Aug 2002 11:20:32 -0400 (EDT)
Subject: Re: HIV in resin - survival?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Whoa. HIV is NOT infective after glutaraldehyde fixation.

I thought the original question was how to preserve it's ultrastructure
for study. It's "morphology" looks just fine after routine fixation.
It's "transmissibility" does not survive fixation. It is a conventional
virus containing RNA and protein that are effectively cross-linked and
inactivated by fixation.

On the other hand, prions (proteinaceous filaments not known to contain
nucleic acids) are not killed by routine fixation. As a matter of fact,
even routine autoclaving does not inactivated them. Killing them requires
high heat for a long time, or chemical treatments like hydroxide, or
formic acid.

Prions cause spongiform encephalopathies (e.g., Bovine Spongiform
Encephalopathy (BSE or mad cow disease), Creutzfeld-Jacob Disease (CJD),
scrapie, chronic wasting disease of deer/elk, and others). Thus, neural
tissue should be handled with care.

I hope this clears up the question.

Sara





Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Aug 9 12:15:40 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Fri, 9 Aug 2002 18:06:17 +0100
Subject: Au corrosion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Apologies for going off-topic, but I have a question for all you corrosion
experts out there.

Will pure Au undergo grain boundary corrosion in a hot (100C) damp
atmosphere? If not, would the presence of atomic nitrogen and/or fluorine
on the surface make a difference?

I always ignorantly assumed that Au didn't corrode at all but I've just seen
some which definately has.

Many thanks indeed,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com



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From daemon Fri Aug 9 14:42:23 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Fri, 9 Aug 2002 15:33:59 -0400
Subject: flatbed scanning with polarized light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael,
By default scanners work by indirect or "darkfield" illumination so there
is no good way to scan by direct reflection. The only possible way this
would work is to tilt the sample relative to the platen and by trial and
error you may get a correct angle. The problem with this is that most of
your sample will be above the platen and probably out of focus. I have no
idea of the depth of field of a typical scanner.
Russ Gillmeister
Xerox
~~~~~~~~~~~~~


-----Original Message-----
} From: michael shaffer [mailto:rarewolf-at-roadrunner.nf.net]
Sent: Friday, August 09, 2002 8:37 AM
To: MicroscopyList


For a multi-faceted project involving many researchers, I wanted to
document a polished sulfide surface for spacial notations and sharing ... I
thought the least I could do is scan the surface with a flatbed. As those
who may have tried this know, the result is very dark because the lamp &
sensor are not in close enough angular alignment. This was a
disappointment, because I wanted to try modifying the scanner with a
polarized sheet of mylar.

My question for the list: Do I rule out ALL flatbed scanners for this?
Has anyone encountered a scanner for which the alignment is close enough for
measuring mirror-like (and anisotropic) relectances??

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From daemon Fri Aug 9 19:13:02 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 09 Aug 2002 17:03:13 -0400
Subject: Re: Source of aluminum corrosion in optical microscope or mirrors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 8/8/02 10:09 PM, Peter Tomic at PTomic-at-anadigics.com wrote:
}
} Since we are on the topic of corrosion & EM, I would like to know if anyone
} near a body of salt water has noticed pitting on front surface coated
} mirrors in "optical" microscopes. To be specific, the type that uses
} visible light and the light is detected by the electromagnetic sensors
} symmetrically spaced about our noses.
}
} I've never been in a lab. that was so close to an ocean that I can attribute
} any corrosion I've ever seen from salt air but I'd like to hear from anyone
} using optical tables [lasers/mirrors] or optical microscopes that knows this
} is a problem, and if shown to be, what is the solution? The only issue I
} have ever had with front surface coated mirror/optics is human fingerprints
} etching the surface or wet chemistry vapors in a fume hood doing the same.
}
Dear Peter,
I don't know about salt air, but, when I was an undergraduate, there was
a 1/10 scale model of the Palomar telescope whose (1st-surface Al) mirror
was severely degraded by the ambient smog. Combined with the--fortunately
temporary--degradation of the electromagnetic sensors, such as you describe,
the overall performance was reduced by a couple of orders of magnitude. The
only solution was to recoat the mirror frequently, but, if the mirror
components are small enough, I'd think that storing them in a desiccator
when not in use would improve the lifetime. A little activated charcoal
might help with the smog, and a desiccant could help with the salt, unless
it was in the form of dry dust--which might not induce corrosion. Since
smog is more-or-less everywhere these days, it could easily be a more
serious problem than salt air.
Yours,
Bill Tivol



From daemon Fri Aug 9 22:48:40 2002



From: Allen Sampson :      ars-at-sem.com
Date: Fri, 9 Aug 2002 22:42:35 -0700
Subject: corrosion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just an aside, but a curious one, to the recent discussions on corrosion.

I had a problem surface a couple of years ago in a couple of similar
vintage, older SEMs (around 25 years old). The lenses used in the cameras,
to image the record CRT on to the film, had developed serious pitting over
the years. The only thing that I have been able to come up with is that
the sulfide bearing negative fixing solutions were always kept near the
camera assembly. The lenses were originally coated, but cleaning over the
years had left scratches in the coatings, and the pitting clearly occurred
along these scratches. Bare aluminum surfaces in the assembly also showed
corrosion.

With more digital imaging these days, this will be less of a problem. But
sometimes, given enough time, the most unexpected sources can cause
problems.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com





From daemon Sat Aug 10 03:39:04 2002



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sat, 10 Aug 2002 03:30:16 -0500
Subject: Re: Source of aluminum corrosion in optical microscope or mirrors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} From: "Bill & Sue Tivol" {wtivol-at-earthlink.net}
: on 8/8/02 10:09 PM, Peter Tomic at PTomic-at-anadigics.com wrote:
: }
: } Since we are on the topic of corrosion & EM, I would like to know if
anyone
: } near a body of salt water has noticed pitting on front surface coated
: } mirrors in "optical" microscopes. To be specific, the type that uses
: } visible light and the light is detected by the electromagnetic sensors
: } symmetrically spaced about our noses.
: }
: } I've never been in a lab. that was so close to an ocean that I can
attribute
: } any corrosion I've ever seen from salt air but I'd like to hear from
anyone
: } using optical tables [lasers/mirrors] or optical microscopes that knows
this
: } is a problem, and if shown to be, what is the solution? The only issue
I
: } have ever had with front surface coated mirror/optics is human
fingerprints
: } etching the surface or wet chemistry vapors in a fume hood doing the
same.
: }
: Dear Peter,
: I don't know about salt air, but, when I was an undergraduate, there
was
: a 1/10 scale model of the Palomar telescope whose (1st-surface Al) mirror
: was severely degraded by the ambient smog. Combined with the--fortunately
: temporary--degradation of the electromagnetic sensors, such as you
describe,
: the overall performance was reduced by a couple of orders of magnitude.
The
: only solution was to recoat the mirror frequently, but, if the mirror
: components are small enough, I'd think that storing them in a desiccator
: when not in use would improve the lifetime. A little activated charcoal
: might help with the smog, and a desiccant could help with the salt, unless
: it was in the form of dry dust--which might not induce corrosion. Since
: smog is more-or-less everywhere these days, it could easily be a more
: serious problem than salt air.

A gold coating over the aluminum of the mirrors should protect them from
corrosion for a longer period of time it the gold coating did not cause
unacceptable changes in the reflected light due to the gold forming a non
linear dielectric mirror. If the gold coating was very thin the problems
should be acceptable for most things.

Gordon
Gordon Couger gcouger-at-couger.com

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward any links or information you think might be useful to others.



From daemon Sat Aug 10 09:29:18 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Sat, 10 Aug 2002 10:26:06 -0400
Subject: Au corrosion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard;

I'm not a corrosion expert and I'd love to hear a good definition of
corrosion from a metallurgist, chemist, quantum physicist or whomever.
However, I do know that Au is "corroded" and/or etched by the halides F-,
Cl-, Br-, and I. I routinely use aqueous potassium iodide to strip Au
metallization from GaAs microcircuits as opposed to "aqua regia" for failure
analysis purposes. Cl is particularly aggressive. Etch rate for KI is
about 20 Angstroms/sec. but will vary with density of the film and
graininess.

Try this experiment. Place one of your devices that is not passivated with
SiN, SiO2 or anything with exposed metal in a closed container with a common
bleach that contains chlorine. You will see how aggressive Cl is to that
gold surface and you may look at the effect on an SEM. It also tends to
blacken the surface. I would imagine this is due to the pitting but have
never had a reason to look deeply into it.

There is bromine in plastic mold compound material used in most commercial
grade integrated circuits and is there as a flame retardant. The volume is
low, ~ 15 PPM, but may not be evenly distributed throughout the plastic
compound so high concentrations can exist in small regions of the compound.
It also creates problems in Al interconnects.

If you are finding Au corrosion to be a reliability issue in your devices,
please talk to me off-line.

Regards,

Peter Tomic
Anadigics, Inc.
Warren, New Jersey
U.S.A.

-----Original Message-----
} From: Richard Beanland [mailto:richard.beanland-at-bookham.com]
Sent: Friday, August 09, 2002 1:06 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Apologies for going off-topic, but I have a question for all you corrosion
experts out there.

Will pure Au undergo grain boundary corrosion in a hot (100C) damp
atmosphere? If not, would the presence of atomic nitrogen and/or fluorine
on the surface make a difference?

I always ignorantly assumed that Au didn't corrode at all but I've just seen
some which definately has.

Many thanks indeed,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com



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From daemon Sat Aug 10 22:26:15 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Sat, 10 Aug 2002 23:20:38 -0400
Subject: Re: corrosion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Allen,

This sounds like the classic example of silver halide that I described in my
previous email on this question. I don't know about the corrosion behavior of
aluminum exposed to thiosulfate (the fixer is not normally a sulfide) or sulfur
dioxide (gas released by oxidation of the fixer) but the silver chloride /
silver bromide of the image layer is certainly going to corrode the aluminum if
particles come off on it.

The same thing is a problem in X-ray diffraction labs that use powder cameras.
Normally one uses a punch to create holes in a strip of film for the X-ray
entry and exit collimators. The cutting edge of the hole punch routinely
corrodes due to being driven into the silver halide emulsion and can begin to
shed rust particles on the film, giving rise to annoying spots.

John Twilley

Allen Sampson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Just an aside, but a curious one, to the recent discussions on corrosion.
}
} I had a problem surface a couple of years ago in a couple of similar
} vintage, older SEMs (around 25 years old). The lenses used in the cameras,
} to image the record CRT on to the film, had developed serious pitting over
} the years. The only thing that I have been able to come up with is that
} the sulfide bearing negative fixing solutions were always kept near the
} camera assembly. The lenses were originally coated, but cleaning over the
} years had left scratches in the coatings, and the pitting clearly occurred
} along these scratches. Bare aluminum surfaces in the assembly also showed
} corrosion.
}
} With more digital imaging these days, this will be less of a problem. But
} sometimes, given enough time, the most unexpected sources can cause
} problems.
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}





From daemon Mon Aug 12 09:58:14 2002



From: Robert Fitton :      fittonro-at-luther.edu
Date: Mon, 12 Aug 2002 08:42:53 -0600
Subject: TEM & SEM available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following scopes are available as a donation to non-profit educational
institutions from Luther College, located in NE Iowa:

Hitachi H300 TEM
ETEC Autoscan

These scopes do not come with required oil rotary pumps.

Email or call Robert Fitton for details at fittonro-at-luther.edu 563-387-1559



Robert Fitton
Teaching Associate/Director of Laboratories
Luther College
Department of Biology
700 College Drive
Decorah, IA 52101

Voice 563-387-1559
FAX 563-387-1080




From daemon Mon Aug 12 10:44:33 2002



From: Hanna Gilbert :      hanna-at-hwr.arizona.edu
Date: Mon, 12 Aug 2002 08:37:17 -0700 (MST)
Subject: Biofilms and metal distributions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi - does anyone have ideas how to quantify metal distributions (Mn, Fe,
Ni, Zn) on MnOx within biofilms? I am dealing with Mn oxidizing bacteria
in an acid-mine drainage contaminated creek in AZ. I would like to be
able to distinguish between the bacteria and algae that are present on
glass slides put out in the creek, if possible, as well as determine the
distributions of the metals (i.e., do they overlay the bacteria?). I
am considering using the LIVE/DEAD BacLight kit from M.Probes w/ CSLM, but
have heard that it stains ALL cells including protozoa, algae, fungi etc.
Is there something that would stain bacteria only such as DAPI or acridine
orange, and then couple that with something to detect the metals? I don't
think there is a probe availabe to detect metals associated with MnOx. It
seems like it may be a multi-step process, and not something that can be
accomplished with just one microscopy technique.

Thanks,

Hanna Gilbert
Hydrology and Water Resources
University of Arizona

----- End forwarded message -----


From daemon Mon Aug 12 14:30:02 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Mon, 12 Aug 2002 15:23:57 -0700
Subject: Re: Hand-held GFP detection devices???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A few years ago we did the following:

took the green filter out of a FITC filter block
took the objective off the microscope
put a dish of GFP expressing yeast or bacteria on the microscope
zapped with blue light from the FITC filter block
held the green filter up to our eyes like a monocle

This worked fine to see which areas of the plate were GFP positive and
which were negative.


At 02:47 PM 7/19/2002 +1000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Aug 12 15:13:14 2002



From: jason-clark-at-uiowa.edu (by way of MicroscopyListserver)
Date: Mon, 12 Aug 2002 15:02:51 -0500
Subject: Ask-A-Microscopist: How to visualize a sodium channel at Cell

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jason-clark-at-uiowa.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
August 12, 2002 at 15:08:36
---------------------------------------------------------------------------

Email: jason-clark-at-uiowa.edu
Name: Jason Clark

Organization: University of Iowa

Education: Graduate College

Location: Iowa City, IA

Question: I am trying to visualize a sodium channel that has a short
half life (~40 min) at the cell membrane. There may be a large pool
of this channel intracellularly (maybe for recycling) and we are
trying to distinguish between the two populations. What is the best
way to view the membrane bound population while excluding the
intracellular population?

Thanks, Jason

---------------------------------------------------------------------------


From daemon Mon Aug 12 15:14:39 2002



From: Mathes, David (TX95) :      David.Mathes-at-honeywell.com
Date: Mon, 12 Aug 2002 15:07:50 -0500
Subject: software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
Before I buy another copy of Adobe PhotoShop, I wanted to see what else
people were using to handle their TEM data. Adobe is great for its
versatility but is there another package that matches this versatility and
that is better suited to microscopy (i.e. having a feature that allows one
to measure the length of features, read pixel values, or generate gray-level
line traces)? Alternatively, are there plug-ins available for Adobe that
let you perform these tasks?

Dave




From daemon Mon Aug 12 15:35:47 2002



From: DrJohnRuss-at-aol.com
Date: Mon, 12 Aug 2002 16:27:25 EDT
Subject: Re: software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 8/12/02 4:21:55 PM, David.Mathes-at-honeywell.com writes:

} Before I buy another copy of Adobe PhotoShop, I wanted to see what else
} people were using to handle their TEM data. Adobe is great for its
} versatility but is there another package that matches this versatility
} and that is better suited to microscopy (i.e. having a feature that allows
} one to measure the length of features, read pixel values, or generate
gray-level
} line traces)? Alternatively, are there plug-ins available for Adobe that
} let you perform these tasks?

There is a complete set of Photoshop plugins that provide the kinds of
processing and measurement tools appropriate for microscopy. Go to
ReindeerGraphics.com and check out Fovea Pro (there is also an online
tutorial that shows some of the functionality).


From daemon Mon Aug 12 17:41:45 2002



From: Earl Weltmer :      earlw-at-sbcglobal.net
Date: Mon, 12 Aug 2002 15:33:11 -0700
Subject: Robinson Backscatter Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I am cleaning house again & find that I have a Robinson Backscatter detector
for an ISI Super II or Super II series SEM.

Free to anyone who has a use & will pay for shipping.

Hope the meeting went well.

Earl Weltmer




From daemon Mon Aug 12 19:05:13 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 12 Aug 2002 17:51:46 -0600
Subject: software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dave,

there are a number of alternative software packages available. Please check
out our web site for more information on our product (analySIS). Other
TEM-specific packages are for example from EmiSpec. Calibration,
measurement, pixel values, line traces, report generation, etc. are all
integrated part of the software.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

Disclaimer: We produce and sell the analySIS family of image analysis
software, and therfore have considerable interest in the product. We are NOT
affiliated with other software mentioned in this email.




-----Original Message-----
} From: Mathes, David (TX95) [mailto:David.Mathes-at-honeywell.com]
Sent: Monday, August 12, 2002 2:08 PM
To: 'microscopy-at-sparc5.microscopy.com'


Hi,
Before I buy another copy of Adobe PhotoShop, I wanted to see what else
people were using to handle their TEM data. Adobe is great for its
versatility but is there another package that matches this versatility and
that is better suited to microscopy (i.e. having a feature that allows one
to measure the length of features, read pixel values, or generate gray-level
line traces)? Alternatively, are there plug-ins available for Adobe that
let you perform these tasks?

Dave




From daemon Tue Aug 13 03:57:48 2002



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Tue, 13 Aug 2002 10:37:28 +0200
Subject: Re: software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

As far as I know Fovea Pro is a set of plugins for Photoshop, which
helps you with some of the image processing you want to do. Dr. John
Russ is involved in the development of this software.

Fovea Pro:
http://www.reindeergraphics.com/products.shtml

I have a list of imaging software on my imaging webpage (scroll down a
bit on the page):

http://ourworld.compuserve.com/homepages/pvosta/pcrimg.htm

Kind regards,

Peter

P.S. I have no connection to or any commercial interest in Fovea Pro.

--
Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)
Cipalstraat 3
B-2440 Geel
Belgium
tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

======================================================================

} -----Original Message-----
} } From: Mathes, David (TX95) [mailto:David.Mathes-at-honeywell.com]
} Sent: Monday, August 12, 2002 2:08 PM
} To: 'microscopy-at-sparc5.microscopy.com'
} Subject: software
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
} Before I buy another copy of Adobe PhotoShop, I wanted to see what else
} people were using to handle their TEM data. Adobe is great for its
} versatility but is there another package that matches this versatility and
} that is better suited to microscopy (i.e. having a feature that allows one
} to measure the length of features, read pixel values, or generate gray-level
} line traces)? Alternatively, are there plug-ins available for Adobe that
} let you perform these tasks?
}
} Dave


From daemon Tue Aug 13 10:10:42 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Tue, 13 Aug 2002 16:47:31 +0200
Subject: HREM simulation of GBs etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I have used the program CrystalKit for building supercells for the
simulation of HREM images of GBs and planar defects. Is there any
competition to this program, or is really the only package worth considering
for this purpose.

Thanks

Ian MacLaren
N.B. New address
TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162894
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Tue Aug 13 11:35:46 2002



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Tue, 13 Aug 2002 12:26:49 -0400
Subject: M&M quebec

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





} Does anyone know what the approximate attendance was at M&M in Quebec.
}
} Greg

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/EM


From daemon Tue Aug 13 11:36:53 2002



From: Phoebe J Doss/app/Cvm :      pjdoss-at-cvm.okstate.edu
Date: Tue, 13 Aug 2002 11:30:39 -0500
Subject: Balzer Med 010 turbo sputter/evaporator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:

I would like to purchase to following item for my coater: specimen holder,
carbon thread or rod evaporator, arm complete and the 2 MC cables. Please
contact me if you have these items or know where I might be able to
purchase them. Thanks.

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Department of Physiological Sciences
264 McElroy Hall
Oklahoma State University
Stillwater, OK 74078
405-744-6765



From daemon Tue Aug 13 13:32:11 2002



From: Larry Hanke :      hanke-at-mee-inc.com
Date: Tue, 13 Aug 2002 13:23:34 -0500
Subject: Re: software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use Paintshop Pro from Jasc Software in our lab for image editing for light
microscopy and SEM. I have been very pleased with its performance. It offers most
of the functionality of Photoshop and is compatible with photoshop plug-ins. We
use the Fovea Pro plug ins for image analysis and have also been pleased with them
for routine measurements.
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


} } Hi,
} } Before I buy another copy of Adobe PhotoShop, I wanted to see what else
} } people were using to handle their TEM data. Adobe is great for its
} } versatility but is there another package that matches this versatility and
} } that is better suited to microscopy (i.e. having a feature that allows one
} } to measure the length of features, read pixel values, or generate gray-level
} } line traces)? Alternatively, are there plug-ins available for Adobe that
} } let you perform these tasks?
} }
} } Dave



From daemon Tue Aug 13 15:16:45 2002



From: ROSSCAC-at-aol.com
Date: Tue, 13 Aug 2002 16:08:35 EDT
Subject: Premade Trumps fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good day to all on the listserver,
I have a question - we are currently having some problems with tissue being
extremely autolyzed. What we have found out is that the person sending us
the tissue uses premade Trumps fixative - they don't check the pH nor the
osmolarity and the fixative comes to them unrefrigerated. I'm not sure how
long they keep the fixative once they get it but I imagine it is longer than
a week - could the wrong pH, hot temperature during shipment, and long
storage be our only problem here? Supposably, they collect the samples
within a reasonable time following exsanguination of the animal - I think
bleeding has something to do with also - does anyone out there have
suggestions or comments that could help us?
Thanks in advance
Connie A. Cummings, DVM, Phd



From daemon Tue Aug 13 16:02:41 2002



From: Baggethun, Paul :      Paul.Baggethun-at-alcoa.com
Date: Tue, 13 Aug 2002 16:55:06 -0400
Subject: software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ImageJ provides all the functionality you mention - and much more. If you
can program in Java, the versatility of this program exceeds almost any
other imaging application. It is free and can bee obtained at
http://rsb.info.nih.gov/ij/docs/index.html However, if you are doing batch
processing involving a large number of images, you'll probably find it too
slow.

"[ImageJ] runs, either as an online applet or as a downloadable application,
on any computer with a Java 1.1 or later virtual machine."

"ImageJ was designed with an open architecture that provides extensibility
via Java plugins. Custom acquisition, analysis and processing plugins can be
developed using ImageJ's built in editor and Java compiler. User-written
plugins make it possible to solve almost any image processing or analysis
problem."

Cheers,
Paul Baggethun
Pittsburgh, PA

-----Original Message-----
} From: Mathes, David (TX95) [mailto:David.Mathes-at-honeywell.com]
Sent: Monday, August 12, 2002 4:08 PM
To: 'microscopy-at-sparc5.microscopy.com'


Hi,
Before I buy another copy of Adobe PhotoShop, I wanted to see what else
people were using to handle their TEM data. Adobe is great for its
versatility but is there another package that matches this versatility and
that is better suited to microscopy (i.e. having a feature that allows one
to measure the length of features, read pixel values, or generate gray-level
line traces)? Alternatively, are there plug-ins available for Adobe that
let you perform these tasks?

Dave




From daemon Tue Aug 13 16:06:02 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 13 Aug 2002 14:54:53 -0600
Subject: M&M quebec

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I heard about 1500 (without vendors). About same as last year. I only heard
this through the grapevine, no guarantees.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Greg Erdos [mailto:gwe-at-biotech.ufl.edu]
Sent: Tuesday, August 13, 2002 10:27 AM
To: Microscopy-at-sparc5.microscopy.com





} Does anyone know what the approximate attendance was at M&M in Quebec.
}
} Greg

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/EM


From daemon Tue Aug 13 17:11:03 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Tue, 13 Aug 2002 18:01:27 -0700
Subject: lectins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



There use to be several companies that sold lectins conjugated to colloidal
gold. Does anyone know of a company that's still selling them?
I appreciate any advice on this matter. Thanks,
Beth Richardson

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Tue Aug 13 20:02:36 2002



From: lillianft-at-aol.com (by way of Ask-A-Microscopist)
Date: Tue, 13 Aug 2002 19:51:25 -0500
Subject: Ask-A-Microscopist: HMDS instead of Critical Point Drying?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lillianft-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
August 13, 2002 at 15:24:37
---------------------------------------------------------------------------

Email: lillianft-at-aol.com
Name: L. Domenico

Organization: CSE

Education: Undergraduate College

Location: City, State, Country

Question: I would like to use HMDS instead of using a critical point
dryer for an SEM course I will be teaching. Experiments that HMDS is
to be used is with plant parts, yeast, bacteria and animal tissue
(mouse jejunum). Are there simple protocols used for HMDS for each
of the specimens mentioned above.Thanks

---------------------------------------------------------------------------


From daemon Tue Aug 13 20:44:35 2002



From: David Rothbard :      rothbardD-at-netscape.net
Date: Tue, 13 Aug 2002 21:37:38 -0400
Subject: Carbon Rod Fixtures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Phoebe:

You can get a "generic" carbon evaporation fixture from Ladd Research.
www.laddresearch.com, catalog chapter 11. I don't know if it will fit
your system. No commercial endorsement implied.

David Rothbard
Earthborn Solutions
rothbardD-at-netscape.net


pjdoss-at-cvm.okstate.edu wrote:

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers:

I would like to purchase to following item for my coater: specimen holder,
carbon thread or rod evaporator, arm complete and the 2 MC cables. Please
contact me if you have these items or know where I might be able to
purchase them. Thanks.

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Department of Physiological Sciences
264 McElroy Hall
Oklahoma State University
Stillwater, OK 74078
405-744-6765




From daemon Wed Aug 14 08:31:33 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 14 Aug 2002 08:21:30 -0500
Subject: antibody

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
We are trying to locate an antibody against elastin to use with sheep cartilage. We would prefer a poly-clonal if possible since we are looking for both unpolymerized and polymerized elastin. I would appreciate any leads as to where we can obtain this.

Thanks,
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Wed Aug 14 09:38:19 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 14 Aug 2002 15:30:15 +0100 (GMT Daylight Time)
Subject: M&M 2001 Proceedings

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I have just enjoyed looking at the 2000 proceedings. Could
someone post the volume and supplement no. for the 2001
proceedings?

Dave

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Aug 14 09:40:56 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 14 Aug 2002 15:34:57 +0100 (GMT Daylight Time)
Subject: Re: Ask-A-Microscopist: HMDS instead of Critical Point Drying?

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The simplest starting point is to dehydrate to 100% ethanol
as usual, keeping to the normal times. Then we use:
ethanol:HMDS 2:1
ethanol:HMDS 1:2
3 washes in 100% HMDS

Remove from HMDS and leave to dry in fume cupboard. If too
small, eg bacteria, let a drop dry on a coverslip.

Dave


On Tue, 13 Aug 2002 19:51:25 -0500 by way of
Ask-A-Microscopist {lillianft-at-aol.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (lillianft-at-aol.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
} August 13, 2002 at 15:24:37
} ---------------------------------------------------------------------------
}
} Email: lillianft-at-aol.com
} Name: L. Domenico
}
} Organization: CSE
}
} Education: Undergraduate College
}
} Location: City, State, Country
}
} Question: I would like to use HMDS instead of using a critical point
} dryer for an SEM course I will be teaching. Experiments that HMDS is
} to be used is with plant parts, yeast, bacteria and animal tissue
} (mouse jejunum). Are there simple protocols used for HMDS for each
} of the specimens mentioned above.Thanks
}
} ---------------------------------------------------------------------------
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Aug 14 09:42:24 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 14 Aug 2002 09:33:35 -0500
Subject: Durst 183 enlarger

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Durst Laborator SM 183 enlarger. Does anyone have a list of lens
configurations for the SM 183? We want to produce nice 8x10 electron
micrographs but nothing we've come up with is satisfactory (ie. spherical
aberration and uneven illumination). All of the lens configurations we have
are for a 138 model. I believe we have objective lenses 180mm, 105mm, and
80mm and condenser lenses 240PT, 240, 200, 130, and 85.

I would really appreciate some help.

Thank you,

Jaclynn M. Lett, Research Technician
Harold W. Siebens Hearing Research Center
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu


From daemon Wed Aug 14 10:43:17 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Wed, 14 Aug 2002 11:31:17 -0400
Subject: Critical temp for SEM operation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anybody knows the max operating room temp for SEM?

Thanks,
Pavel



From daemon Wed Aug 14 11:10:15 2002



From: sghoshro-at-NMSU.Edu
Date: Wed, 14 Aug 2002 10:02:08 -0600 (MDT)
Subject: Re: lectins

Contents Retrieved from Microscopy Listserver Archives
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Beth,

Try this link

http://www.inbios.com/conjugatedgold.html

InBios International
562 First Avenue South, Suite #600
Seattle, WA 98104
Phone: 206.344.5821 Fax: 206.344.5823

No financial interest in InBios.

Good luck,

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml

On Tue, 13 Aug 2002, Beth Richardson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} There use to be several companies that sold lectins conjugated to colloidal
} gold. Does anyone know of a company that's still selling them?
} I appreciate any advice on this matter. Thanks,
} Beth Richardson
}
} **********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} **********************************************************************
}
} "And it's only the giving that makes you what you are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
} ***************************************************************************
}
}
}
}



From daemon Wed Aug 14 13:36:24 2002



From: Mathes, David (TX95) :      David.Mathes-at-honeywell.com
Date: Wed, 14 Aug 2002 13:25:58 -0500
Subject: EM facilities

Contents Retrieved from Microscopy Listserver Archives
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Hi folks,
I am looking for an EM facility within a few hundred miles of Dallas
TX that would be willing to rent out time on their equipment to industry.
Specifically, I would like access to a FIB and a TEM (preferably 200keV or
better).
Thanks
Dave




From daemon Wed Aug 14 13:38:02 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 14 Aug 2002 11:59:54 -0700
Subject: Re: Critical temp for SEM operation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our scope starts to behave badly above 29 deg. C or so.

Ron L
----- Original Message -----
} From: "ATC SEM Laboratory" {atcsem-at-earthlink.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 14, 2002 11:31 AM


I'm assuming you are asking about SEM equipment rather
than protocols, specimens, etc.

Different manufacturers have their own set of environmental
specs. I would presume that these are known-good ranges
where no problems would occur. But each maker's units,
and perhaps each unit, would have different max limits.
If the SEM is computer controlled or even has processors
inside, that is another dimension besides issues of
regular electronics. Then, one has to consider whether the
lens coils and/or HV supply are water cooled. The lens
drivers are, as far as I know, always air cooled. Same for
all the other electronics.

I run my SEM at room conditions between 68-72F, 25-38% RH.
One time, when my A/C was dirty, temp got to 82F. SEM still worked.
Here in CA, with the iffy power, early last year I installed two
6KVA double conversion UPS units--one for console, one
for column. These run the system for about two hours with
the console off. The issue was to keep the Zr/W FE gun
running during power outages. The down side is that these
UPS units increase heat load in the SEM room since they
are in the same room. So, if the A/C were to fail, room temp
would rise dramatically.

Nevertheless, I have not reached an upper temperature
where the system quit working. And definitely not a limit
where the system broke. So to answer your question--
it depends.

gary


At 08:31 AM 8/14/2002, you wrote:

} Does anybody knows the max operating room temp for SEM?
}
} Thanks,
} Pavel



From daemon Wed Aug 14 14:42:34 2002



From: Stephen Black :      black-at-mshri.on.ca
Date: Wed, 14 Aug 2002 17:27:38 -0400
Subject: GFP Lighting

Contents Retrieved from Microscopy Listserver Archives
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Phoebe Doss,

Our custom evaporation fixtures can be found on our web site as David
pointed out. The direct address is
http://www.laddresearch.com/General_Catalog/Chapter_11/chapter_11.html

I am sure ours could be used in your system with the proper feed through.
Please contact me directly if you are interested.

Thanks,

John Arnott
Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "David Rothbard" {rothbardD-at-netscape.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, August 13, 2002 9:37 PM


Hello Everyone,
There has been an increasing number of enquiries regarding lighting systems
for fluorescence emission viewing.
Our lab is using a number of different systems from BLS, a Hungarian company
with many years of experience in the manufacture of biological lab
equipment.
If you would like to visit the web site, this is the address;
http://www.bls-ltd.com/
Regards to all,
Stephen Black

Technical Manager
Nagylab
Rm. 881
Samuel Lunenfeld Research Institute
Mount Sinai Hospital
600 University Ave.
Toronto, M5G 1X5
Canada

tel. (416)-586-8455 Lab
fax (416)-586-8588
URL: http://www.mshri.on.ca/nagy/
email: black-at-mshri.on.ca




From daemon Wed Aug 14 16:50:36 2002



From: billiams-at-virgilio.it ()
Date: Wed, 14 Aug 2002 14:39:45 -0700
Subject: Is it really possible to seduce menm8s6h1h6

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(billiams-at-virgilio.it) on Wednesday, August 14, 2002 at 14:39:45
---------------------------------------------------------------------------

: It's not a secret anymore... There is a NEW product available in the United States and it is the strongest formula ever sold! Pheromones! Make your partner HOT!Click the link below to get PRIMAL! Click here: http://getit-at-www.friendlymailer.com/index9987.php?marketing_id=via011 Click to remove http://www.spambites.com/cgi-bin/enter.cgi?spambytes_id=21574

---------------------------------------------------------------------------



From daemon Wed Aug 14 17:38:44 2002



From: jinsong wu :      jinsong.wu-at-asu.edu
Date: Wed, 14 Aug 2002 15:22:00 -0700
Subject: summary of "liquid He transferring kids"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Lister,

Soon after the M&M conference, I wish to give a detailed summary on
the question of liquid He transferring kids.

1. It seems that continuous filling of liquid-He is impossible right
now, since:
} There are two main disadvantages with continuous filling, one is the helium
} loss along a long transfer tube, the second is vibration and goniometer
} movement problems. Because of the double walled design of transfer tubes,
} they are quite stiff and during transfer there is a lot of turbulence at
the
} exhaust which will destroy any resolution (comments by Bobert Morrison).

2. Another good and detailed suggestion was given by Ron Doole:

} We do not loose vacuum when filling but we do support the transfer tube to
} prevent too much strain on the holder.
} The initial fill takes about 45 minutes. It is best for 2 people to set up
} and remove the He transfer tube, although it is possible for a single
} person to do it it just seems safer with two.
} Depending on temperature the hold time is generally about 50 mins to 3
} hours (50 mins at 7K or 8K, 2 hours at 15K, 2.5 hours at 21K etc.).

} Subsequent transfers take about 30 minutes.
} As we tend to use the holder infrequently and book a week of He stage work
} when we want it we will always repump the vacuum jackets on the transfer
} dewar and holder before each session.

} The most diffucult thing is to determine when the dewar is full and on the
} first trial we shot most of a 50l dewar of He through the holder as we
} could not tell the difference between the He gas plume and the He liquid
} plume on the exhaust line. With experience we can now fill with about 5L
} of He.
} It is important to use the gas to cool the holder down to a reasonable
} level (50K) to avoid loosing too much liquid.

3. Some points concering whether the holder is filled enough (provided by
Robert Morrison):
} It looks as if the holder is not being filled enough, as on test here the
} hold time was over 1 hour at base temp. Is the dewar well pumped? In
} principle when He is filled it should cryopump well so a good rotary pump
} vacuum should be sufficient. Does the pressure relief valve blow off during
} use showing a high heat load?

I greatly appreciate these kind suggestions and comments. And I wish the
person
who has also interest in the problem can share their experiences. Still, the
question
is open for more discussion.

With Best Regards,

Jinsong Wu
Department of Physics and Astronomy
Arizona State University
Tempe, AZ 85287-1504

Tel: 480-965-2535 (o)





From daemon Wed Aug 14 19:19:18 2002



From: Cavin Mooers :      cavinm-at-vsl.cua.edu (by way of MicroscopyListserver)
Date: Wed, 14 Aug 2002 19:07:29 -0500
Subject: TEM CCD Imaging - Seeking User Insights to Various Manufacturers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists,

I am currently in the market for CCD camera for a TEM. I have obtained
all the literature and pricing info regarding the various products, but
would like some user opinions on performance and quality of service,
(especially service.)

I would greatly appreciate any insight anyone may have to offer.

Sincerely,


Cavin Mooers, EM Facility Manager
Vitreous State Laboratory
The Catholic University of America
Hannan Hall
Washington, DC 20064
(202) 319-5346phone
(202) 319-4469fax


From daemon Wed Aug 14 19:40:04 2002



From: fkriss-at-chem.udel.edu (by way of Ask-A-Microscopist)
Date: Wed, 14 Aug 2002 19:29:54 -0500
Subject: Ask-A-Microscopist:Looking for Stains for....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (fkriss-at-chem.udel.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
August 14, 2002 at 14:03:17
---------------------------------------------------------------------------

Email: fkriss-at-chem.udel.edu
Name: Frank Kriss

Organization: Univ. of Delaware

Education: Undergraduate College

Location: Newark, DE, USA

Question: I am searching stains for amino acids, lysine, and NH3+
groups in TEM work. Does anyone know a good reference for this type
of work?

---------------------------------------------------------------------------


From daemon Wed Aug 14 20:20:58 2002



From: Venture Direct :      ffmail-at-ven.com
Date: Wed, 14 Aug 2002 18:15:11 -0700
Subject: Economical force measurement pressure film sample kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Engineer:

Pressurex force indicating sensor film is now available in a new trial-size package. Immediately address your tactile surface stress issues by trying the Pressurex force indicating film sample kit for only $149.

Pressurex sensor film is a thin, flexible sheet (physically similar in appearance to a magazine page) of microencapsulated mylar that instantaneously and permanently changes color in proportion to the amount of force applied upon it. Like Litmus paper, the resultant color changes are quantifiable and can be compared to a calibration table to determine actual PSI values. Applications include examination of gasketed surfaces, bolted joints and clamps, nip impressions, heat seals, lamination presses, composite layups, electronic packaging and more.

The sample kit consists of a one foot sheet of each of Pressurex's six PSI ranges, from 2 PSI to 19,000 PSI, an instruction manual and color calibration tables.

To order: Tel: 973.560.9092, Fax: 973.884.1699, Email: bebner-at-sensorprod.com

Bill Ebner
Sensor Products Inc. USA
www.sensorprod.com


} From time to time we will send you information about
valuable new services, products and special offers
from Sensor Products and our preferred business partners
that we feel will be of interest to you.

If you do not want to receive these emails please
click on the unsubscribe link below.

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From daemon Wed Aug 14 20:20:59 2002



From: Venture Direct :      ffmail-at-ven.com
Date: Wed, 14 Aug 2002 18:15:31 -0700
Subject: Economical force measurement pressure film sample kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Engineer:

Pressurex force indicating sensor film is now available in a new trial-size package. Immediately address your tactile surface stress issues by trying the Pressurex force indicating film sample kit for only $149.

Pressurex sensor film is a thin, flexible sheet (physically similar in appearance to a magazine page) of microencapsulated mylar that instantaneously and permanently changes color in proportion to the amount of force applied upon it. Like Litmus paper, the resultant color changes are quantifiable and can be compared to a calibration table to determine actual PSI values. Applications include examination of gasketed surfaces, bolted joints and clamps, nip impressions, heat seals, lamination presses, composite layups, electronic packaging and more.

The sample kit consists of a one foot sheet of each of Pressurex's six PSI ranges, from 2 PSI to 19,000 PSI, an instruction manual and color calibration tables.

To order: Tel: 973.560.9092, Fax: 973.884.1699, Email: bebner-at-sensorprod.com

Bill Ebner
Sensor Products Inc. USA
www.sensorprod.com


} From time to time we will send you information about
valuable new services, products and special offers
from Sensor Products and our preferred business partners
that we feel will be of interest to you.

If you do not want to receive these emails please
click on the unsubscribe link below.

Unsubscribe from Sensor Products -
http://www.xactmail.com/html/sp-optout.htm

Please note that replying to this email will not
unsubscribe you; you must click on the link above
in order to remove your name from this list.



From daemon Wed Aug 14 20:20:59 2002



From: Venture Direct :      ffmail-at-ven.com
Date: Wed, 14 Aug 2002 18:15:29 -0700
Subject: Economical force measurement pressure film sample kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Engineer:

Pressurex force indicating sensor film is now available in a new trial-size package. Immediately address your tactile surface stress issues by trying the Pressurex force indicating film sample kit for only $149.

Pressurex sensor film is a thin, flexible sheet (physically similar in appearance to a magazine page) of microencapsulated mylar that instantaneously and permanently changes color in proportion to the amount of force applied upon it. Like Litmus paper, the resultant color changes are quantifiable and can be compared to a calibration table to determine actual PSI values. Applications include examination of gasketed surfaces, bolted joints and clamps, nip impressions, heat seals, lamination presses, composite layups, electronic packaging and more.

The sample kit consists of a one foot sheet of each of Pressurex's six PSI ranges, from 2 PSI to 19,000 PSI, an instruction manual and color calibration tables.

To order: Tel: 973.560.9092, Fax: 973.884.1699, Email: bebner-at-sensorprod.com

Bill Ebner
Sensor Products Inc. USA
www.sensorprod.com


} From time to time we will send you information about
valuable new services, products and special offers
from Sensor Products and our preferred business partners
that we feel will be of interest to you.

If you do not want to receive these emails please
click on the unsubscribe link below.

Unsubscribe from Sensor Products -
http://www.xactmail.com/html/sp-optout.htm

Please note that replying to this email will not
unsubscribe you; you must click on the link above
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From daemon Wed Aug 14 21:44:18 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 14 Aug 2002 22:41:28 -0400
Subject: Critical temp for SEM operation

Contents Retrieved from Microscopy Listserver Archives
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Pavel;

I would think that it's the max. operating temperature of the microscopist
that you need to concern yourself with. Your chiller should be able to keep
the temp. stable for all inhabitable conditions. If it is too hot for the
electronics, the microscopist will have been deceased long before an
electronic failure.

Peter Tomic

-----Original Message-----
} From: ATC SEM Laboratory [mailto:atcsem-at-earthlink.net]
Sent: Wednesday, August 14, 2002 11:31 AM
To: Microscopy-at-sparc5.microscopy.com


Does anybody knows the max operating room temp for SEM?

Thanks,
Pavel



From daemon Wed Aug 14 23:44:59 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 14 Aug 2002 20:59:47 -0700
Subject: Re: TEM CCD Imaging - Seeking User Insights to Various

Contents Retrieved from Microscopy Listserver Archives
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Cavin

You have to decide first: are you going to use optical coupling camera or
direct fiber-optic coupling? First type is faster and good for nearly
video speed (real time imaging); second type is slower, but has better
sensitivity and image quality (no distortions from optics). Second: do you
want bottom or top (35 mm port) mount camera? Bottom mount camera is
essential for high resolution work, but limited by small field. Top mount
camera has bigger field and good for routine work. Third: retractable or
not. If retractable - you may use film as well, if non-retractable
bottom-mount - forget the film. Most modern cameras are retractable now.
When I shop for my camera I find that this market is relatively small, so
you don't expect fast service. I mean: they simply don't have enough
people in most cases for quick response. And last: check software - you
may find very good camera coming with terrible, user unfriendly soft... My
advise: always ask for demo (sorry, camera guys) before final decision;
took your sample and make your own pictures, compare images from different
cameras side by side, you would be surprised to see how different they
could be. You may contact me off line if need details. Sergey

At 05:07 PM 8/14/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Aug 15 02:58:03 2002



From: Stuart Kearns :      Stuart.Kearns-at-bristol.ac.uk
Date: Thu, 15 Aug 2002 08:47:49 +0100
Subject: Re: Critical temp for SEM operation

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 14 Aug 2002 14:27:44 -0400 Ron L'Herault {lherault-at-bu.edu}
wrote:
}
} Our scope starts to behave badly above 29 deg. C or so.
}
} Ron L

Our users start to behave badly above 25 deg C.

Stu
--------------------------------------------
Stuart Kearns
Electron Microbeam Laboratories
Department of Earth Sciences
University of Bristol
Queens Road
Bristol BS8 1RJ
UK
tel: (0)117 954 5435
fax: (0)117 925 3385
e-mail: Stuart.Kearns-at-bristol.ac.uk
http://eugf.gly.bris.ac.uk
--------------------------------------------



From daemon Thu Aug 15 06:47:07 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 15 Aug 2002 12:37:31 +0100 (GMT Daylight Time)
Subject: Re: Critical temp for SEM operation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We hit 38deg C last summer. Users complained of feeling
sleepy. The service engineer said the electronics should
not go above 40 deg. C. If it was 38 on the desk the
electonics would be hotter.

Dave


On Thu, 15 Aug 2002 08:47:49 +0100 Stuart Kearns
{Stuart.Kearns-at-bristol.ac.uk} wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} On Wed, 14 Aug 2002 14:27:44 -0400 Ron L'Herault {lherault-at-bu.edu}
} wrote:
} }
} } Our scope starts to behave badly above 29 deg. C or so.
} }
} } Ron L
}
} Our users start to behave badly above 25 deg C.
}
} Stu
} --------------------------------------------
} Stuart Kearns
} Electron Microbeam Laboratories
} Department of Earth Sciences
} University of Bristol
} Queens Road
} Bristol BS8 1RJ
} UK
} tel: (0)117 954 5435
} fax: (0)117 925 3385
} e-mail: Stuart.Kearns-at-bristol.ac.uk
} http://eugf.gly.bris.ac.uk
} --------------------------------------------
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Aug 15 09:11:13 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 15 Aug 2002 08:02:44 -0600
Subject: Re: TEM CCD Imaging - Seeking User Insights to Various

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A SEM is less critical than a TEM in respect to temperature.

A chiller is used to cool down parts of the microscope acting as an
heatsink.

Every mechanical part is changing, as slightly as you want, dimensions with
temperature.

Usually, all mechanical measuring instruments (and parts) are tested at the
temperature of 20 degree Celsius (I do not know the Imperial standard for
such a measurement).

Since this temperature is not exactly very comfortable for the human been,
the best thing is to get the microscope in a room with about 24 - 25 degree
Celsius, but the important thing is that this temperature is not changing
very much and very fast.

Try to imagine the trouble to take a high magnification picture while the
stage is warming up or cooling down changing its dimension of 500 nanometres
in
one hour, and on your screen 1 centimetre represents 50 nanometres.

The mechanical parts of the column too behave the same, but as stated at the
beginning, a SEM is less sensitive than a TEM.



Marco Arienti



www.electronrescue.com



----- Original Message -----
} From: "ATC SEM Laboratory" {atcsem-at-earthlink.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 14, 2002 5:31 PM


Cavin,

Sergey makes a lot of good points, but one point needs correction:

Bottom-mount cameras are usually attached BELOW the film chamber and thus do
NOT impede the use of film, retractable or not. The only time you need a
retractable bottom-mount camera is if you have other instrments attached at
the bottom port and need to use both.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, August 14, 2002 10:00 PM
To: Cavin Mooers; Microscopy-at-sparc5.microscopy.com


Cavin

You have to decide first: are you going to use optical coupling camera or
direct fiber-optic coupling? First type is faster and good for nearly
video speed (real time imaging); second type is slower, but has better
sensitivity and image quality (no distortions from optics). Second: do you
want bottom or top (35 mm port) mount camera? Bottom mount camera is
essential for high resolution work, but limited by small field. Top mount
camera has bigger field and good for routine work. Third: retractable or
not. If retractable - you may use film as well, if non-retractable
bottom-mount - forget the film. Most modern cameras are retractable now.
When I shop for my camera I find that this market is relatively small, so
you don't expect fast service. I mean: they simply don't have enough
people in most cases for quick response. And last: check software - you
may find very good camera coming with terrible, user unfriendly soft... My
advise: always ask for demo (sorry, camera guys) before final decision;
took your sample and make your own pictures, compare images from different
cameras side by side, you would be surprised to see how different they
could be. You may contact me off line if need details. Sergey

At 05:07 PM 8/14/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Aug 15 10:46:54 2002



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG (by way of
Date: Thu, 15 Aug 2002 10:30:46 -0500
Subject: Antibody-elastin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Debby,

I've noticed several requests on the ListServer re: antibodies. I
refer all of
you to Linscott's Directory of Immunological and Biological Reagents
(http://www.linscott'sdirectory.com or
http://www.linscott'sdirectory.co.uk. It
is an excellent source of monoclonal and polyclonal antibodies, etc. Plus, it
will direct you to the suppliers of those antibodies, etc.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org


From daemon Thu Aug 15 12:23:14 2002



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 15 Aug 2002 03:04:56 -0700
Subject: RE: Critical temp for SEM operation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That depends on a number of variables. If you rely on the dimensional
certifiable calibration of the SEM, you may have limitations, depending on
the circuit design. But the primary consideration is generally the
combination of temperature, humidity and if water cooling or EDS is used.

High humidity is a limitation if an EDS system is in use, as the
cryogenically cooled EDS 'snout' in the sample chamber will condense water
vapor whenever the chamber is vented in a high humidity environment. This
will give you problems in pump down times.

If any water cooling is used in the electronics or vacuum systems, the
cooling lines can condense water that would be harmful to the systems.
Trust me, I have charged thousands to repair damage caused by small
amounts of water introduced to electronic circuits.

I have assumed (perhaps wrongly) that by high temperatures you mean a lack
of control of the ambient environment. In other words, an area that
doesn't have air conditioning. If the instrument is operated in a high
temperature environment that doesn't also have a high humidity, you are
only accelerating the normal lifetime of the electronics. When you bring a
high humidity into the environment you are introducing a variety of effects
that can bring the whole system down in ways you can't imagine.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, August 14, 2002 8:31 AM, ATC SEM Laboratory
[SMTP:atcsem-at-earthlink.net] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anybody knows the max operating room temp for SEM?
}
} Thanks,
} Pavel
}
}
}
}



From daemon Thu Aug 15 15:17:39 2002



From: robert.fowler-at-tdktca.com
Date: Thu, 15 Aug 2002 16:12:44 -0400
Subject: Critical temp for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



While on the subject. Does anyone have any comments on humidity in and
around a SEM?

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com


THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY
TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL
INFORMATION.

If you are not the intended recipient, be advised that any use,
dissemination, distribution or duplication of this transmission is strictly
prohibited. If you received this transmission in error, please notify the
sender immediately by electronic reply to this transmission or by phone
(847-803-6100). Thank you.




From daemon Thu Aug 15 20:37:51 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 15 Aug 2002 18:29:19 -0700
Subject: RE: TEM CCD Imaging - Seeking User Insights to Various

Contents Retrieved from Microscopy Listserver Archives
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You right, Mike. I am sorry. Sergey

At 07:02 AM 8/15/02, you wrote:
} Cavin,
}
} Sergey makes a lot of good points, but one point needs correction:
}
} Bottom-mount cameras are usually attached BELOW the film chamber and thus do
} NOT impede the use of film, retractable or not. The only time you need a
} retractable bottom-mount camera is if you have other instrments attached at
} the bottom port and need to use both.
}
} mike
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Wednesday, August 14, 2002 10:00 PM
} To: Cavin Mooers; Microscopy-at-sparc5.microscopy.com
} Subject: Re: TEM CCD Imaging - Seeking User Insights to Various
} Manufacturers
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Aug 16 07:42:56 2002



From: Lauren Chesnut :      CHESNUTL-at-uthscsa.edu
Date: Fri, 16 Aug 2002 07:36:36 -0500
Subject: CAP-proficiency testing of clinical EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Could I please get everyone who handles clinical cases to pass on how they
deal with the issue of CAP- proficiency testing.
I am unaware of any "official testing" of clinical EM labs. Does anyone know
of one? How do you address this issue in your Procedure Manual? Thanks for
all your help.

Lauren E. Chesnut
Technical Director
Electron Microscopy Lab
University of Texas Health-
Science Center at San Antonio
Dept. of Pathology
(210)567-4052
chesnutl-at-uthscsa.edu





From daemon Fri Aug 16 08:44:59 2002



From: Matyas Buzgo :      buzgo-at-systbot.unizh.ch
Date: Fri, 16 Aug 2002 09:35:12 -0400
Subject: WHAT IS SUPERFROST

Contents Retrieved from Microscopy Listserver Archives
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Hye Community

Does anybody know the difference between

A) Superfrost and Poly-L-Lysine
B) Superfrost and Silanated coatings

for Microscopy glass slide coating for in situ hybridizations?

Answering directly to buzgo-at-systbot.unizh.ch is welcome.

Thanks for all

Matyas





From daemon Fri Aug 16 09:10:15 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Fri, 16 Aug 2002 10:03:26 -0400
Subject: RE: Antibody-elastin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In regards to the web address of the linscott's directory make sure you type
linscotts: www.linscottsdirectory.com

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954





Debby,

I've noticed several requests on the ListServer re: antibodies. I
refer all of
you to Linscott's Directory of Immunological and Biological Reagents
(http://www.linscott'sdirectory.com or
http://www.linscott'sdirectory.co.uk. It
is an excellent source of monoclonal and polyclonal antibodies, etc. Plus,
it
will direct you to the suppliers of those antibodies, etc.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org


From daemon Fri Aug 16 10:41:03 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Fri, 16 Aug 2002 17:29:37 +0200
Subject: Timeframe to establish a EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

Here is a interesting question. What is the average time to set up a
complete electron Microscope Laboratory. This include portioning,
workbenches, electrical connections, Gas connections as well as TEM, SEM,
CLSM for both the biological as well as Material science field.

Furthermore what is a realistic timeframe to have it all commissioned,
running smoothly and producing publications as well as getting the new
academic community accustomed to the abilities?

Thanks




From daemon Fri Aug 16 14:47:08 2002



From: JHoffpa464-at-aol.com
Date: Fri, 16 Aug 2002 15:36:34 -0400
Subject: Re: Timeframe to establish a EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


this is a joke question, right?


From daemon Fri Aug 16 15:02:43 2002



From: robert.fowler-at-tdktca.com
Date: Fri, 16 Aug 2002 16:00:28 -0400
Subject: Nikon Eclipse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello Listers

Do any of the users of the Nikon Eclipse have a recommended cleaning
procedure for the optics. I have a spot of dust or other material viewed
through the eyepiece and camera This has been present after one month of
purchase and I used it to "trademark" pictures taken from this scope. But
it is now an annoyance. Another problem I had was my light source became
darker over time. The problem was not in filament degradation but in the
first optic which spreads or concentrates the beam. This optic had a haze
of some sort. Easily cleaned off but now has me concerned about the high
humidity present in the lab. Has anyone experienced this?


Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com


THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY
TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL
INFORMATION.

If you are not the intended recipient, be advised that any use,
dissemination, distribution or duplication of this transmission is strictly
prohibited. If you received this transmission in error, please notify the
sender immediately by electronic reply to this transmission or by phone
(847-803-6100). Thank you.




From daemon Fri Aug 16 19:14:24 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Fri, 16 Aug 2002 20:05:57 -0400 (EDT)
Subject: Re: Nikon Eclipse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fluorescence or brightfield?

We found with our TE200 that a collection filter near the Hg arc lamp
popped out because the epoxy holding it in got brittle. The images
had dark areas. The precise location of the problem took us a
long time to figure out because of the way it fell inside the lamp housing
opening.

People who use too much oil cause it to drip onto the filter blocks.
These dark spots are easily cleaned.

Basically, to find dirt, just go through the light path looking at every
surface. Because of infinity correction, anything between the back of the
objective and the camera can be particulary problematic.

___________________________________
WORK: http://www.aecom.yu.edu/aif/




From daemon Fri Aug 16 21:48:09 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 16 Aug 2002 22:44:34 -0400
Subject: Timeframe to establish a EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Your question encompasses a great deal of ground so I will simply tell you
my experience.

We moved into a second building in 1998 that was abandoned for years prior
to us taking possession. That is, it was literally open to the sky with
holes in the ceiling and had to be renovated from the ground up, everything
but the concrete foundation which fortunately was nice and thick, 16 inches,
and very stable. The process of restoring this building took a year but it
was in deplorable condition. I will assume you are starting with a building
that is inhabitable, clean and has climate/humidity control.

We had to do a site survey after the building was in order for
electromagnetic fields and floor stability with a seismic instrument. This
took just a few days. Fortunately there were no issues. This is not always
the case if you are near large horsepower electric motors, electric closets,
elevators or heavy vehicular traffic. You may need to mitigate these
conditions if adverse and that may be time consuming if you need structural
modifications [permits required in most places] to the building like a
"floating" floor, structural members or electromagnetic isolation/shielding
or getting someone to move a soda machine away from the SEM column. The
soda machine people never like this and generally don't care about your SEM
nor your scientific pursuits. Fortunately, in our case, none of these
issues arose.

If you have a chiller for the SEM you won't have to deal with a chilled
water supply. Chilled house water supply could be time consuming,
plumbing, permits, etc. Gasses such as CDA [clean dry air], nitrogen and
vacuum, if not already in the lab., may take several weeks to install
depending on where the feeds are if there are any at all.

I purchased a Hitachi S4500/2 FESEM, dropped it into place and with the help
of Hitachi's field service folks was able to use the instrument in 5 days.
In short, after the utilities were in and the SEM delivered, we were
functional with few problems that were facility related. Vacuum systems are
another issue entirely.

The time to get an academic community accustomed to anything is essentially
a function of the community and not the instrumentation or facility. I'd
like to hear back from you in a year or so on that one.

I hope this is of some help and I would imagine others on this listserver
have additional experiences to share.

Good luck.

Peter Tomic
Anadigics, Inc.


-----Original Message-----
} From: Coetzee, Mr S. H Physics Science [mailto:COETZEES-at-mopipi.ub.bw]
Sent: Friday, August 16, 2002 11:30 AM
To: Microscopy-at-sparc5.microscopy.com


Dear all

Here is a interesting question. What is the average time to set up a
complete electron Microscope Laboratory. This include portioning,
workbenches, electrical connections, Gas connections as well as TEM, SEM,
CLSM for both the biological as well as Material science field.

Furthermore what is a realistic timeframe to have it all commissioned,
running smoothly and producing publications as well as getting the new
academic community accustomed to the abilities?

Thanks




From daemon Mon Aug 19 06:17:20 2002



From: Re Marilena :      marilena.re-at-brindisi.enea.it
Date: Mon, 19 Aug 2002 12:58:53 +0200
Subject: Re: HREM simulation of GBs etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*This message was transferred with a trial version of CommuniGate(tm) Pro*
Dear Ian, I am interested in HRTEM images simulation.
I do not know if I can help you. I would like to know what kind of computer
program you use after the generation of the supercell in order to simulate the
HRTEM images. I have used EMS program, but first I have to generate a supercell
file (where I indicate all the information present in a normal crystal file,
which can be create with EMS itself) by myself: this file should have a well
defined extension you have to respect in order to apply the sub-program to
reproduce the effect of the microscope. Also with Cerius2 program you have to
write a well defined extension before simulating the effect of the microscope.
Could you please give me some more indication about the program you have used to
create the supercell? It should be very useful for me because it is often
necessary to use a supercell to simulate the real specimens of which the
scientific world is interested.
Thanks
Marilena

Ian MacLaren wrote:

} *This message was transferred with a trial version of CommuniGate(tm) Pro*
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear all,
} I have used the program CrystalKit for building supercells for the
} simulation of HREM images of GBs and planar defects. Is there any
} competition to this program, or is really the only package worth considering
} for this purpose.
}
} Thanks
}
} Ian MacLaren
} N.B. New address
} TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
} Petersenstr. 23, 64287 Darmstadt, Germany
} Tel: +49 6151 162894
} ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Mon Aug 19 08:26:34 2002



From: J-H Lignot :      J-H.Lignot-at-c-strasbourg.fr
Date: Mon, 19 Aug 2002 15:22:09 +0200
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938


From daemon Mon Aug 19 09:22:22 2002



From: Anaspec Luc Harmsen :      luc-at-anaspec.co.za
Date: Mon, 19 Aug 2002 16:14:23 +0200
Subject: ICEM 15 photograph competition - Own a Kruger Rand

Contents Retrieved from Microscopy Listserver Archives
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Micrograph Competition :: The Art of Microscopy
A micrograph competition will be held at ICEM-15 to highlight the art of
science. The competition will be open to all forms of microscopic imaging.
Winners will be selected on artistic merit and general audience appeal. The
image may be a single image or a montage of several images. The rules of
entry are:
Any individual may submit a (maximum) of two entries.
Entries must have an overall dimension of 27.5cm x 35.0cm, and can be
mounted vertically or horizontally. Entries must be affixed to a stiff
lightweight support, such as 10mm foam board, and may be mounted so as to
have borders.
Each entry must have a separate text sheet 23cm x 30cm with a title centred
at the top of the page, followed by two single carriage returns, and a 200
word, maximum, description of the image. Times Roman 14pt font is
recommended.
The entrant’s name, address and image title must be on the back of the
micrograph mount.
Entries must be brought to the congress and mounted on the competition
display board by noon on Monday 2 September and must be removed between
09h00 and 12h00 on Friday morning. Micrographs remaining on the display
board after this will be donated to local schools.
Prizes for the winning micrographs will be awarded at the banquet on
Thursday evening.
So please don't forget to pack those pictures before you step on to the
plane.

Luc Harmsen
Marketing ICEM15 www.ICEM15.com
1-6 September 2002
Durban, South Africa




From daemon Mon Aug 19 09:37:25 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Mon, 19 Aug 2002 09:27:02 -0500
Subject: RE: Durst 183 enlarger

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you all for your help with my Durst SM 183 enlarger questions.

Jaclynn M. Lett, Research Technician
Harold W. Siebens Hearing Research Center
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu


From daemon Mon Aug 19 10:48:57 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 19 Aug 2002 11:26:58 -0400
Subject: FEI Technai 12 Users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning All,
I am looking for some last-minute, off-list, advice from any of you
who have Technai 12's up and running. Ours is due in 3 weeks.

Thanks,

Fred

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center, Room SS024
West Chester University of Pennsylvania
South Church Street & Rosedale Ave.
West Chester, PA, 19383
Phone & FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/


From daemon Mon Aug 19 11:08:35 2002



From: Lauren Chesnut :      chesnut-at-pathology.v20.uthscsa.edu
Date: Mon, 19 Aug 2002 11:04:53 -0500
Subject: CAP-proficiency testing

Contents Retrieved from Microscopy Listserver Archives
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I apologize for re-posting this message, I have had trouble with my e-mail.
How do clinical labs deal with the College of American Pathologist
inspection question about proficiency testing of the lab?


Lauren E. Chesnut
Technical Director
Electron Microscopy Lab
University of Texas
Health Science Center
at San Antonio
7703 Floyd Curl Drive
San Antonio, TX. 78229-3900
Dept. of Pathology
(210)567-4052



From daemon Mon Aug 19 11:34:43 2002



From: Dominik Hezel :      d.hezel-at-uni-koeln.de
Date: Mon, 19 Aug 2002 18:27:39 +0200
Subject: keatite sample

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Dear all,

I hope, this isn't a crossposting: I recently analyzed different silica
modifications in meteorites. I got one spectra I could not identify. It
isn't qtz, trd, crs, cs, stishovite, melanophlogite or moganite. From the
spectra we can say that the modification should have low symmetry and low
specific density. Therefore we think it could be keatite. This mineral often
occurs in hydrothermal experiments between 300 and 600°C. The exact
stability field is not known. I am know looking for a keatite sample, to
take a raman spectra and compare it to the one I obtained. And there is my
question: Can anyone lend me a sample of keatite? It is not important,
whether it is within a thin section or a grain. The sample can be quite
small, as we use microraman. The sample will not be destroyed and does not
need any preparation and will certainly send back pretty soon.

It would obviously be better, if someone has a raman spectra of keatite,
which I could have. I can also send my spectra to anyone who is interested
and can maybe say something about it. Or - even better - does anyone has a
raman spectra data base of different silica modifications? Who knows, what
we found maybe isn't keatite at all ...

best regards,

Dominik Hezel


_____________
Dominik Hezel
Institut für Mineralogie und Geochemie
der Universität zu Köln
Zülpicherstr. 49b
50674 Köln
Tel.: +49 (0221) 470 32 28
e-mail: d.hezel-at-uni-koeln.de



From daemon Mon Aug 19 12:14:44 2002



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Mon, 19 Aug 2002 13:05:57 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938


From daemon Mon Aug 19 13:47:13 2002



From: Karen Rethoret :      rethoret-at-yorku.ca
Date: Mon, 19 Aug 2002 15:29:39 -0400 (Eastern Daylight Time)
Subject: Hitachi Evaporator Manual Req'd

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ann -

I'm sure this is not very OSH-friendly, but when we do Au-Pd evaporation
(we never do carbon-coating any more), the tungsten filament in the
evaporator glows white-hot, and I suspect that it's not at all good for you.
We have a plexiglass "implosion protection shield" - sort of a big cylinder
with a closed upper end that fits over the evaporator's bell jar. This is
supposed to contain flying shards of glass should the bell jar ever implode.
I sincerely hope it never happens....
Anyway, as far as protecting your eyes from the bright light, we've
simply placed a few strips of masking tape around the implosion protection
shield at the level(s) that people would be looking through, and it does a
good job of shielding the glare. You can still see what's going on during
the evaporation process. You should probably avoid sticking anything to the
bell jar itself - anything that may induce the slightest extra stress in a
bell jar (especially an old one) under high vacuum is not good.
No doubt some kind of dark safety glasses would be better, as a safety
measure, I'm sure, but an actual welder's mask may be a bit of overkill. Of
course, it would be good implosion protection....

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada

----- Original Message -----
} From: "Lehman, Ann" {Ann.Lehman-at-trincoll.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, August 19, 2002 2:05 PM



Hello,

We have acquired a vintage but healthy Hitachi Vacuum Evaporator, model
HUS-4.
Since it requires all manual operation of valves etc., I would really like
to find a copy of the operator's manual if one exists.

Please reply directly to me

Karen Rethoret
Biology Department
York University
4700 Keele St.
Toronto, Ontario
M3J 1P3

rethoret-at-yorku.ca
416-736-2100 x33289



From daemon Mon Aug 19 14:39:14 2002



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 19 Aug 2002 15:30:40 -0400 (EDT)
Subject: Re: CAP-proficiency testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What you need to do, since CAP doesn¹t send out a specific QC test for EM,
is to design a program yourself. The method is unspecified. They will
accept a genuine effort on your part to examine your product and ensure
that it is first class.

What we do is to send out micrographs, along with clinical histories, to a
competent electron microscopist at other institutions. (CAP likes to see
interaction between labs, rather than just asking a pathologist at your
own institution to look at your work). This needs to be done every 6
months. We have 3 different folks who review our work (2-5 cases, one to
5 micrographs/case), each person once every 18 months (only one reviewer
every 6 months). They send back a report that basically says that the
work is acceptable for the purpose that it is designed. One is a
pathologist, and the other two are PhD lab directors. Look at the CAP
questions and design a questionnaire for the reviewer that asks whether
certain standards are met. Make it as easy as possible for the reviewer;
i.e., don¹t ask for a written report; just make a checklist of things like
quality and correct diagnosis. Also, include a place for signature and
title of the reviewer. Include a stamped, self-addressed envelope for
return of the micrographs and questionnaire. Send it to another lab
director or physician with some familiarity with the process.

Hope this helps.


Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Mon Aug 19 15:54:54 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 19 Aug 2002 16:39:01 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Arc welding glass works very well once the arc is struck and you can watch the carbon rods decrease very nicely. You can just buy the glass for the helmet and it works just fine. One of the EM supply houses (EMS or SPI?) has them in their catalog.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
Sent: Monday, August 19, 2002 1:06 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938


From daemon Mon Aug 19 16:21:34 2002



From: Charles Murphy :      murphyc-at-ba.ars.usda.gov
Date: Mon, 19 Aug 2002 17:09:12 -0400
Subject: broken refrigerator, chemical salvage??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi: Our refrigerator/freezer broke while I was on vacation. The chemicals and samples felt warm to the touch upon return. They were without cold temps for 2-14 days. Can they be salvaged or should I just start over. A list of chem below:

Osmium tetroxide (100% crystal & 4% aqueous in ampules)
Glutaraldehyde and paraformaldehyde (10-70% ampules)
Goat Serum
Various anti-bodies
LR Whites resin

Thanks for any input, Charlie Murphy


Charles Murphy
USDA, ARS,
Electron Microscopy Unit
Bldg. 177B, BARC East
Beltsville, MD, 20705
(301) 504-8046
(301) 504-8923 fax
murphyc-at-ba.ars.usda.gov






From daemon Mon Aug 19 18:11:29 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 19 Aug 2002 16:03:14 -0700
Subject: Re: Eye protection during C-evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A welder's helmet would certainly do the job. Not only
does it reduce the intensity but it removes lots of UV.

There are some new high-tech units that use a controllable
LCD do adjust the amount of opacity. I presume that it too
reduces UV. Welding supply houses carry these accessories.

gary


At 10:05 AM 8/19/2002, you wrote:

} Dear Listers,
}
} For many years, I've used nothing but self-discipline to avoid direct eye
} contact with the white-hot electrodes resulting during a carbon evaporation
} run. However, times have changed and now I am responsible for the safety of
} student users.
}
} Does anyone know whether an arc-welder's face-shield would protects the eyes
} from this type of intense light? My impression is that the face-shield
} mainly protects the welder from emitted sparks, and I don't know whether the
} light emission is similar to that from carbon evaporation.
}
} If not, what eye protection are others using out there in List-land?
}
} Thanks as always for your help.
}
} Ann
}
} ###################
} Ann Hein Lehman
} Assistant Director, EM Facility
} Trinity College - LSC314
} 300 Summit Street
} Hartford CT 06106
} v. 860-297-4289
} f. 860-297-2538
} e. ann.lehman-at-trincoll.edu
} w. http://www.trincoll.edu/~alehman/
} Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
}
}
} -----Original Message-----
} } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
} Sent: Monday, August 19, 2002 9:22 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Administrivia: June 2002 Archives are now On-Line
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Aug 19 22:10:30 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Mon, 19 Aug 2002 22:00:48 -0500
Subject: Electron Microscopy Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,

I am posting the following message from Richard Cartun in case any of
you knew David Knibbs and didn't hear about his death and
in case any of you might be able to help Dr. Cartun out in
finding someone who could carry on as lab director. If anyone would
like more information, please contact Dr. Cartun at the address and
phone below or at his e-mail address:
Rcartun-at-harthosp.org

Regards,
Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas

Richard Cartun wrote:
It is with great sadness that I announce the untimely death of my friend
and colleague, David R. Knibbs, Ph.D. He collapsed and died after an
evening run (one of his joys in life) on Monday, August 5th. David (49
years old) was our Director of Electron Microscopy and worked with me
very closely here at Hartford Hospital. Like me, David completed his
Ph.D. studies in Pathobiology at the University of Connecticut. We will
miss him enormously.

Now, we must begin our search for a replacement (I'm not sure that is
the right word since I don't believe David will ever be replaced) and I
must turn to the Histonet for help. We feel it is imperative to
maintain our quality EM service here at our hospital, but realize that
finding a qualified director who will also be called upon to do all the
technical work (David was a "one man show") will be most difficult.
Please contact me if you have any suggestions or recommendations.
Thank you.

Richard Cartun, Ph.D.
Director, Immunopathology
Co-Director, Histology
Hartford Hospital
Hartford, CT 06102

(860) 545-1596



From daemon Tue Aug 20 05:23:58 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Tue, 20 Aug 2002 12:09:48 +0200
Subject: broken refrigerator, chemical salvage??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Charles

I know this sound a bit rough, but here is our horror story. When I arrived
here at the University of Botswana all our chemical was not in a fridge for
6 months. This include temperatures above 36 degrees! Sofar our LR white
is still working fine after being refrigerated again as well as the Osmium
and Glute.

At least some chemicals appear to be a bit more robust that suggested.

Hope this helps



-----Original Message-----
} From: Charles Murphy [mailto:murphyc-at-ba.ars.usda.gov]
Sent: Monday, August 19, 2002 11:09 PM
To: Microscopy-at-sparc5.microscopy.com


Hi: Our refrigerator/freezer broke while I was on vacation. The chemicals
and samples felt warm to the touch upon return. They were without cold temps
for 2-14 days. Can they be salvaged or should I just start over. A list of
chem below:

Osmium tetroxide (100% crystal & 4% aqueous in ampules)
Glutaraldehyde and paraformaldehyde (10-70% ampules)
Goat Serum
Various anti-bodies
LR Whites resin

Thanks for any input, Charlie
Murphy


Charles Murphy
USDA, ARS,
Electron Microscopy Unit
Bldg. 177B, BARC East
Beltsville, MD, 20705
(301) 504-8046
(301) 504-8923 fax
murphyc-at-ba.ars.usda.gov






From daemon Tue Aug 20 06:22:12 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Tue, 20 Aug 2002 13:14:00 +0200
Subject: TEM: Lorentz microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Has anyone done Lorentz microscopy in a Philips CM20? If so,
have you any good practical tips as to how I should adjust the
microscope for optimal performance in this purpose? How should I adjust
the lenses to minimise the field at the sample, for instance?

Thanks for all the help you can give.

Best wishes

Ian MacLaren
TU-Darmstadt
Darmstadt
Germany



From daemon Tue Aug 20 07:25:44 2002



From: Frank Bungartz :      FRANK.BUNGARTZ-at-asu.edu (by way of
Date: Tue, 20 Aug 2002 07:13:10 -0500
Subject: Reichert Jung 975C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,
we have an old Reichert-Jung Cryostat Freezing Microtome Model 975C.
The microtome still works perfect but the blade is getting dull. Does
anyone know whom to contact for a re-sharpening of the blades or
evenentually for a blade replacement?
Thanks very much.
Yours,
Frank

_____________________________________

Frank Bungartz
Lichen Herbarium
Department of Plant Biology
Arizona State University (ASU)
PO Box 87 1601
Tempe, AZ 85287 - 1601
USA

e-mail: frank.bungartz-at-asu.edu
(or bungartz-at-imap3.asu.edu)

phone:
(480) 965 7133 (herbarium)
(480) 965 7735 (laboratory)
fax:
(480) 965 6899


From daemon Tue Aug 20 07:41:28 2002



From: John Arnott :      ladres-at-worldnet.att.net
Date: Tue, 20 Aug 2002 08:35:00 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ann,

For in house use we use goggles, which we also sell along with our
evaporators. You can check them out at
http://www.laddresearch.com/General_Catalog/Chapter_7/Safety_Aids/Personal_P
rotection/personal_protection.html

JD Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Lehman, Ann" {Ann.Lehman-at-trincoll.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, August 19, 2002 1:05 PM


Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938





From daemon Tue Aug 20 10:41:47 2002



From: Paul.Nolan-at-alcan.com
Date: Tue, 20 Aug 2002 11:32:07 -0400
Subject: Pseudo-leather glove

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


[First off, thanks to all the replies I received regarding FTIR microscopy
(I'm still working on it)]

What vendors sell "psuedo-leather" gloves. Canadian vendors would be best.
Please contact me off line

Thanks

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



From daemon Tue Aug 20 10:51:38 2002



From: Yuquan Ding :      yding-at-uwaterloo.ca
Date: Tue, 20 Aug 2002 11:50:55 -0400
Subject: Installation of old detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

Our new detector has been damaged. I will try to install our
old detector in JMS-840 SEM for a temporary use, before I can get a new
detector. This old detector (Link Pentafet plus detector) was used in the
same SEM around one and half years ago. Parameters of the old detector are
given in the following:

Model: 6276
Det. Area: 10.0 mmxmm
Window: 0.008
Resol.: 137 ev at 5.99 ekv
Serial: 1080-3929
Att. No.: 32cc32088

The manual shows the “EXCHANGE DETECTOR” procedure. But this procedure
does not indicate details on filling liquid nitrogen. If anybody of you has
experience with installation of this type of detectors, I would like to have
your opinions and thoughts. This detector has not used for one and half
years. Also I have some questions on installation of the detector as
follows.

1) When liquid nitrogen will be filled? I think that first the detector
assembly will be fixed in the SEM, and then liquid nitrogen will be filled
until the chamber vacuum is ready.
2) I don't know if the old detector can be thermally cycled. What procedure
(warm up & cool down?) will be performed during filling liquid nitrogen? Or
an easy way is just to fill liquid nitrogen, then and wait for the
next calibration after four hours ?

Any suggestions and recommendation on installation of this old detector will
be greatly appreciated. Thank you for your help.

Yuquan Ding
Dept of Mechanical Engineering
University of Waterloo
Waterloo, ON Canada N2L 3G1
Tel: 519-888-4567 ext 3766



From daemon Tue Aug 20 10:56:48 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 20 Aug 2002 08:50:30 -0700
Subject: Re: Eye protection during C-evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ann,
For years I have used a pair of welder's goggles with my vacuum evaporator.
Since there is no danger of sparks there is no need for full head protection
and they are a little easier to wear than the full welder's helmet, so the
students are more likely to put them on. You are correct in assuming that
the UV from the arc is dangerous to the eyes and may contrubute to cataract
formation.
At 01:05 PM 08/19/2002 -0400, you wrote:
}
}
} Dear Listers,
}
} For many years, I've used nothing but self-discipline to avoid direct eye
} contact with the white-hot electrodes resulting during a carbon evaporation
} run. However, times have changed and now I am responsible for the safety of
} student users.
}
} Does anyone know whether an arc-welder's face-shield would protects the eyes
} from this type of intense light? My impression is that the face-shield
} mainly protects the welder from emitted sparks, and I don't know whether the
} light emission is similar to that from carbon evaporation.
}
} If not, what eye protection are others using out there in List-land?
}
} Thanks as always for your help.
}
} Ann
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Aug 20 12:59:12 2002



From: Helen Ackison :      midnite-at-swbell.net
Date: 20 Aug 02 19:53:21 -0000
Subject: thank you for your time

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To whom it may concern:

I am a business man in Dallas Texas up until recently I have been a very successful one. please let me start off by telling you this is not a plea, I am not begging I am simply offering a great business opportunity to anyone who has the foresight and guts to take a chance here is a little of where I am coming from.

I am forty years old and I have worked hard all of my life I have a beautiful wife and three fantastic kids I have everything I need in life including having accepted Jesus Christ as my lord and savior. my children are all happy and healthy and my marriage is fantastic as a matter of fact we are celebrating our twentieth anniversary soon.

due to some tragedies in my life that I will explain if we talk I have taken a financial loss that I have not been able to get back up from. my back is against the wall I am paying over two thousand dollars a week to people I should not have borrowed from but I do pay it every week. I am able when I am not starting from this deep in the hole to make great amounts of money in short periods of time so here is my proposition.

I need forty thousand dollars to pay off all of the friends who have helped me all of the sharks I borrowed from and to live on until I get going again.

in return I will pay this loan back in installments of 2000.00 a month for forty months that is a one hundred percent return on your investment. this is not a scam I will meet you in person I have excellent character and business references that will tell you I am a man of my word. I am not asking for bank accounts or wires all transactions will be done in person if you can.

like I said, if you have foresight and guts, I promise you will not regret it please only serious inquires only this a legitimate business offer. I will only do this with one person. so if you are at all interested please email me at:

equitablems-at-eurosport.com

I know this sounds crazy but believe me I have tried everything else there is and until I get out of the hole I just aint gonna make it. thanking you in advance, LC

god bless everyone this gets to whether you are able to help or not.



From daemon Tue Aug 20 14:08:06 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 19 Aug 2002 16:39:01 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
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Arc welding glass works very well once the arc is struck and you can watch the carbon rods decrease very nicely. You can just buy the glass for the helmet and it works just fine. One of the EM supply houses (EMS or SPI?) has them in their catalog.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
Sent: Monday, August 19, 2002 1:06 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


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Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938



From daemon Tue Aug 20 14:08:06 2002



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Mon, 19 Aug 2002 13:05:57 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

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Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


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Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938



From daemon Tue Aug 20 14:12:28 2002



From: Charles Murphy :      murphyc-at-ba.ars.usda.gov
Date: Mon, 19 Aug 2002 17:09:12 -0400
Subject: broken refrigerator, chemical salvage??

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Hi: Our refrigerator/freezer broke while I was on vacation. The chemicals and samples felt warm to the touch upon return. They were without cold temps for 2-14 days. Can they be salvaged or should I just start over. A list of chem below:

Osmium tetroxide (100% crystal & 4% aqueous in ampules)
Glutaraldehyde and paraformaldehyde (10-70% ampules)
Goat Serum
Various anti-bodies
LR Whites resin

Thanks for any input, Charlie Murphy


Charles Murphy
USDA, ARS,
Electron Microscopy Unit
Bldg. 177B, BARC East
Beltsville, MD, 20705
(301) 504-8046
(301) 504-8923 fax
murphyc-at-ba.ars.usda.gov







From daemon Tue Aug 20 15:11:02 2002



From: Karen Rethoret :      rethoret-at-yorku.ca
Date: Mon, 19 Aug 2002 15:29:39 -0400 (Eastern Daylight Time)
Subject: Hitachi Evaporator Manual Req'd

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Hello,

We have acquired a vintage but healthy Hitachi Vacuum Evaporator, model
HUS-4.
Since it requires all manual operation of valves etc., I would really like
to find a copy of the operator's manual if one exists.

Please reply directly to me

Karen Rethoret
Biology Department
York University
4700 Keele St.
Toronto, Ontario
M3J 1P3

rethoret-at-yorku.ca
416-736-2100 x33289




From daemon Tue Aug 20 15:11:08 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 19 Aug 2002 16:03:14 -0700
Subject: Re: Eye protection during C-evaporation

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A welder's helmet would certainly do the job. Not only
does it reduce the intensity but it removes lots of UV.

There are some new high-tech units that use a controllable
LCD do adjust the amount of opacity. I presume that it too
reduces UV. Welding supply houses carry these accessories.

gary


At 10:05 AM 8/19/2002, you wrote:

} Dear Listers,
}
} For many years, I've used nothing but self-discipline to avoid direct eye
} contact with the white-hot electrodes resulting during a carbon evaporation
} run. However, times have changed and now I am responsible for the safety of
} student users.
}
} Does anyone know whether an arc-welder's face-shield would protects the eyes
} from this type of intense light? My impression is that the face-shield
} mainly protects the welder from emitted sparks, and I don't know whether the
} light emission is similar to that from carbon evaporation.
}
} If not, what eye protection are others using out there in List-land?
}
} Thanks as always for your help.
}
} Ann
}
} ###################
} Ann Hein Lehman
} Assistant Director, EM Facility
} Trinity College - LSC314
} 300 Summit Street
} Hartford CT 06106
} v. 860-297-4289
} f. 860-297-2538
} e. ann.lehman-at-trincoll.edu
} w. http://www.trincoll.edu/~alehman/
} Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
}
}
} -----Original Message-----
} } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
} Sent: Monday, August 19, 2002 9:22 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Administrivia: June 2002 Archives are now On-Line
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Tue Aug 20 15:15:58 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Mon, 19 Aug 2002 22:00:48 -0500
Subject: Electron Microscopy Position

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Hello everyone,

I am posting the following message from Richard Cartun in case any of
you knew David Knibbs and didn't hear about his death and
in case any of you might be able to help Dr. Cartun out in
finding someone who could carry on as lab director. If anyone would
like more information, please contact Dr. Cartun at the address and
phone below or at his e-mail address:
Rcartun-at-harthosp.org

Regards,
Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas

Richard Cartun wrote:
It is with great sadness that I announce the untimely death of my friend
and colleague, David R. Knibbs, Ph.D. He collapsed and died after an
evening run (one of his joys in life) on Monday, August 5th. David (49
years old) was our Director of Electron Microscopy and worked with me
very closely here at Hartford Hospital. Like me, David completed his
Ph.D. studies in Pathobiology at the University of Connecticut. We will
miss him enormously.

Now, we must begin our search for a replacement (I'm not sure that is
the right word since I don't believe David will ever be replaced) and I
must turn to the Histonet for help. We feel it is imperative to
maintain our quality EM service here at our hospital, but realize that
finding a qualified director who will also be called upon to do all the
technical work (David was a "one man show") will be most difficult.
Please contact me if you have any suggestions or recommendations.
Thank you.

Richard Cartun, Ph.D.
Director, Immunopathology
Co-Director, Histology
Hartford Hospital
Hartford, CT 06102

(860) 545-1596




From daemon Tue Aug 20 15:21:26 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Tue, 20 Aug 2002 12:09:48 +0200
Subject: broken refrigerator, chemical salvage??

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Ann -

I'm sure this is not very OSH-friendly, but when we do Au-Pd evaporation
(we never do carbon-coating any more), the tungsten filament in the
evaporator glows white-hot, and I suspect that it's not at all good for you.
We have a plexiglass "implosion protection shield" - sort of a big cylinder
with a closed upper end that fits over the evaporator's bell jar. This is
supposed to contain flying shards of glass should the bell jar ever implode.
I sincerely hope it never happens....
Anyway, as far as protecting your eyes from the bright light, we've
simply placed a few strips of masking tape around the implosion protection
shield at the level(s) that people would be looking through, and it does a
good job of shielding the glare. You can still see what's going on during
the evaporation process. You should probably avoid sticking anything to the
bell jar itself - anything that may induce the slightest extra stress in a
bell jar (especially an old one) under high vacuum is not good.
No doubt some kind of dark safety glasses would be better, as a safety
measure, I'm sure, but an actual welder's mask may be a bit of overkill. Of
course, it would be good implosion protection....

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada

----- Original Message -----
} From: "Lehman, Ann" {Ann.Lehman-at-trincoll.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, August 19, 2002 2:05 PM


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Dear Charles

I know this sound a bit rough, but here is our horror story. When I arrived
here at the University of Botswana all our chemical was not in a fridge for
6 months. This include temperatures above 36 degrees! Sofar our LR white
is still working fine after being refrigerated again as well as the Osmium
and Glute.

At least some chemicals appear to be a bit more robust that suggested.

Hope this helps



-----Original Message-----
} From: Charles Murphy [mailto:murphyc-at-ba.ars.usda.gov]
Sent: Monday, August 19, 2002 11:09 PM
To: Microscopy-at-sparc5.microscopy.com


Hi: Our refrigerator/freezer broke while I was on vacation. The chemicals
and samples felt warm to the touch upon return. They were without cold temps
for 2-14 days. Can they be salvaged or should I just start over. A list of
chem below:

Osmium tetroxide (100% crystal & 4% aqueous in ampules)
Glutaraldehyde and paraformaldehyde (10-70% ampules)
Goat Serum
Various anti-bodies
LR Whites resin

Thanks for any input, Charlie
Murphy


Charles Murphy
USDA, ARS,
Electron Microscopy Unit
Bldg. 177B, BARC East
Beltsville, MD, 20705
(301) 504-8046
(301) 504-8923 fax
murphyc-at-ba.ars.usda.gov







From daemon Tue Aug 20 15:29:20 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 19 Aug 2002 11:26:58 -0400
Subject: FEI Technai 12 Users

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Morning All,
I am looking for some last-minute, off-list, advice from any of you
who have Technai 12's up and running. Ours is due in 3 weeks.

Thanks,

Fred

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center, Room SS024
West Chester University of Pennsylvania
South Church Street & Rosedale Ave.
West Chester, PA, 19383
Phone & FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



From daemon Tue Aug 20 15:44:54 2002



From: Frank Bungartz :      FRANK.BUNGARTZ-at-asu.edu (by way of
Date: Tue, 20 Aug 2002 07:13:10 -0500
Subject: Reichert Jung 975C

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Dear Microscopists,
we have an old Reichert-Jung Cryostat Freezing Microtome Model 975C.
The microtome still works perfect but the blade is getting dull. Does
anyone know whom to contact for a re-sharpening of the blades or
evenentually for a blade replacement?
Thanks very much.
Yours,
Frank

_____________________________________

Frank Bungartz
Lichen Herbarium
Department of Plant Biology
Arizona State University (ASU)
PO Box 87 1601
Tempe, AZ 85287 - 1601
USA

e-mail: frank.bungartz-at-asu.edu
(or bungartz-at-imap3.asu.edu)

phone:
(480) 965 7133 (herbarium)
(480) 965 7735 (laboratory)
fax:
(480) 965 6899



From daemon Tue Aug 20 15:45:28 2002



From: Anaspec Luc Harmsen :      luc-at-anaspec.co.za
Date: Mon, 19 Aug 2002 16:14:23 +0200
Subject: ICEM 15 photograph competition - Own a Kruger Rand

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Micrograph Competition :: The Art of Microscopy
A micrograph competition will be held at ICEM-15 to highlight the art of
science. The competition will be open to all forms of microscopic imaging.
Winners will be selected on artistic merit and general audience appeal. The
image may be a single image or a montage of several images. The rules of
entry are:
Any individual may submit a (maximum) of two entries.
Entries must have an overall dimension of 27.5cm x 35.0cm, and can be
mounted vertically or horizontally. Entries must be affixed to a stiff
lightweight support, such as 10mm foam board, and may be mounted so as to
have borders.
Each entry must have a separate text sheet 23cm x 30cm with a title centred
at the top of the page, followed by two single carriage returns, and a 200
word, maximum, description of the image. Times Roman 14pt font is
recommended.
The entrant’s name, address and image title must be on the back of the
micrograph mount.
Entries must be brought to the congress and mounted on the competition
display board by noon on Monday 2 September and must be removed between
09h00 and 12h00 on Friday morning. Micrographs remaining on the display
board after this will be donated to local schools.
Prizes for the winning micrographs will be awarded at the banquet on
Thursday evening.
So please don't forget to pack those pictures before you step on to the
plane.

Luc Harmsen
Marketing ICEM15 www.ICEM15.com
1-6 September 2002
Durban, South Africa





From daemon Tue Aug 20 15:51:12 2002



From: Dominik Hezel :      d.hezel-at-uni-koeln.de
Date: Mon, 19 Aug 2002 18:27:39 +0200
Subject: keatite sample

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Dear all,

I hope, this isn't a crossposting: I recently analyzed different silica
modifications in meteorites. I got one spectra I could not identify. It
isn't qtz, trd, crs, cs, stishovite, melanophlogite or moganite. From the
spectra we can say that the modification should have low symmetry and low
specific density. Therefore we think it could be keatite. This mineral often
occurs in hydrothermal experiments between 300 and 600°C. The exact
stability field is not known. I am know looking for a keatite sample, to
take a raman spectra and compare it to the one I obtained. And there is my
question: Can anyone lend me a sample of keatite? It is not important,
whether it is within a thin section or a grain. The sample can be quite
small, as we use microraman. The sample will not be destroyed and does not
need any preparation and will certainly send back pretty soon.

It would obviously be better, if someone has a raman spectra of keatite,
which I could have. I can also send my spectra to anyone who is interested
and can maybe say something about it. Or - even better - does anyone has a
raman spectra data base of different silica modifications? Who knows, what
we found maybe isn't keatite at all ...

best regards,

Dominik Hezel


_____________
Dominik Hezel
Institut für Mineralogie und Geochemie
der Universität zu Köln
Zülpicherstr. 49b
50674 Köln
Tel.: +49 (0221) 470 32 28
e-mail: d.hezel-at-uni-koeln.de




From daemon Tue Aug 20 16:11:41 2002



From: Lauren Chesnut :      chesnut-at-pathology.v20.uthscsa.edu
Date: Mon, 19 Aug 2002 11:04:53 -0500
Subject: CAP-proficiency testing

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I apologize for re-posting this message, I have had trouble with my e-mail.
How do clinical labs deal with the College of American Pathologist
inspection question about proficiency testing of the lab?


Lauren E. Chesnut
Technical Director
Electron Microscopy Lab
University of Texas
Health Science Center
at San Antonio
7703 Floyd Curl Drive
San Antonio, TX. 78229-3900
Dept. of Pathology
(210)567-4052




From daemon Tue Aug 20 16:11:42 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Mon, 19 Aug 2002 09:27:02 -0500
Subject: RE: Durst 183 enlarger

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Thank you all for your help with my Durst SM 183 enlarger questions.

Jaclynn M. Lett, Research Technician
Harold W. Siebens Hearing Research Center
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu



From daemon Tue Aug 20 16:13:39 2002



From: Re Marilena :      marilena.re-at-brindisi.enea.it
Date: Mon, 19 Aug 2002 12:58:53 +0200
Subject: Re: HREM simulation of GBs etc

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*This message was transferred with a trial version of CommuniGate(tm) Pro*
Dear Ian, I am interested in HRTEM images simulation.
I do not know if I can help you. I would like to know what kind of computer
program you use after the generation of the supercell in order to simulate the
HRTEM images. I have used EMS program, but first I have to generate a supercell
file (where I indicate all the information present in a normal crystal file,
which can be create with EMS itself) by myself: this file should have a well
defined extension you have to respect in order to apply the sub-program to
reproduce the effect of the microscope. Also with Cerius2 program you have to
write a well defined extension before simulating the effect of the microscope.
Could you please give me some more indication about the program you have used to
create the supercell? It should be very useful for me because it is often
necessary to use a supercell to simulate the real specimens of which the
scientific world is interested.
Thanks
Marilena

Ian MacLaren wrote:

} *This message was transferred with a trial version of CommuniGate(tm) Pro*
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear all,
} I have used the program CrystalKit for building supercells for the
} simulation of HREM images of GBs and planar defects. Is there any
} competition to this program, or is really the only package worth considering
} for this purpose.
}
} Thanks
}
} Ian MacLaren
} N.B. New address
} TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung
} Petersenstr. 23, 64287 Darmstadt, Germany
} Tel: +49 6151 162894
} ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/




From daemon Tue Aug 20 16:21:35 2002



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 19 Aug 2002 15:30:40 -0400 (EDT)
Subject: Re: CAP-proficiency testing

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What you need to do, since CAP doesn¹t send out a specific QC test for EM,
is to design a program yourself. The method is unspecified. They will
accept a genuine effort on your part to examine your product and ensure
that it is first class.

What we do is to send out micrographs, along with clinical histories, to a
competent electron microscopist at other institutions. (CAP likes to see
interaction between labs, rather than just asking a pathologist at your
own institution to look at your work). This needs to be done every 6
months. We have 3 different folks who review our work (2-5 cases, one to
5 micrographs/case), each person once every 18 months (only one reviewer
every 6 months). They send back a report that basically says that the
work is acceptable for the purpose that it is designed. One is a
pathologist, and the other two are PhD lab directors. Look at the CAP
questions and design a questionnaire for the reviewer that asks whether
certain standards are met. Make it as easy as possible for the reviewer;
i.e., don¹t ask for a written report; just make a checklist of things like
quality and correct diagnosis. Also, include a place for signature and
title of the reviewer. Include a stamped, self-addressed envelope for
return of the micrographs and questionnaire. Send it to another lab
director or physician with some familiarity with the process.

Hope this helps.


Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Tue Aug 20 16:26:07 2002



From: John Arnott :      ladres-at-worldnet.att.net
Date: Tue, 20 Aug 2002 08:35:00 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

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Ann,

For in house use we use goggles, which we also sell along with our
evaporators. You can check them out at
http://www.laddresearch.com/General_Catalog/Chapter_7/Safety_Aids/Personal_P
rotection/personal_protection.html

JD Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Lehman, Ann" {Ann.Lehman-at-trincoll.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, August 19, 2002 1:05 PM


Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938






From daemon Tue Aug 20 16:30:15 2002



From: J-H Lignot :      J-H.Lignot-at-c-strasbourg.fr
Date: Mon, 19 Aug 2002 15:22:09 +0200
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938



From daemon Tue Aug 20 16:30:16 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Tue, 20 Aug 2002 13:14:00 +0200
Subject: TEM: Lorentz microscopy

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
Has anyone done Lorentz microscopy in a Philips CM20? If so,
have you any good practical tips as to how I should adjust the
microscope for optimal performance in this purpose? How should I adjust
the lenses to minimise the field at the sample, for instance?

Thanks for all the help you can give.

Best wishes

Ian MacLaren
TU-Darmstadt
Darmstadt
Germany




From daemon Tue Aug 20 18:48:32 2002



From: Frank Bungartz :      FRANK.BUNGARTZ-at-asu.edu (by way of
Date: Tue, 20 Aug 2002 18:35:46 -0500
Subject: Reichert Microtome Blade

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,
thanks a lot to all of you for the helpful answers where to get the
Reichert Microtome Blade sharpened.
Yours,
Frank

_____________________________________

Frank Bungartz
Lichen Herbarium
Department of Plant Biology
Arizona State University (ASU)
PO Box 87 1601
Tempe, AZ 85287 - 1601
USA

e-mail: frank.bungartz-at-asu.edu
(or bungartz-at-imap3.asu.edu)

phone:
(480) 965 7133 (herbarium)
(480) 965 7735 (laboratory)
fax:
(480) 965 6899


From daemon Tue Aug 20 21:06:11 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 20 Aug 2002 21:48:18 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


And I, I of the old school, use my genuine, official, AO-Polaroid, WWII,
flying-ace, flight glasses. Whenever the C-sun gets brighter, I just turn
the knob and cross my polarizers. And, they were free!!!!

For the students, there is nothing so flashy. They get good, pre-tested,
welders goggles, but there is nothing like crossed and crosser polarizers.

Unfortunately, I've lost the optics from my submarine periscope and I can't
use my goggles from around the corner of the room. I am now thinking of the
old wooden box with two mirrors trick.

As for self-discipline, I lost that sometime during my wedding night, but I
have been searching for it ever since. And, like so many [old(?)] men after
35+ years of marriage, I hurry home each night to buss the wife and smile my
secret smiles - still clueless about it all. And while the Sry gene finally
gives some meaning to the existence of the Y chromosome, we who have been
thus pre-determined feel short-changed without the ability to choose, that
having been taken from us by an arbitrary sequence of nucleotides for which
we neither asked nor were given the power or right to exorcise. Yet, still,
I humbly count my blessings. I could have been born a Medaca, with two Y's,
and still been twice oppositely determined, in an estrogenized aquarium, to
carry XX plumbing. Would that have been an example of one unfulfilled
expectation, or would that count as two?

"And now", she says, "he feels content, having new company in his clueless
abyss where no one knows what makes a man - or a woman". And, if your baby
Medacas are born with "incorrect" chromosomes, you may merely have to spike
the water to set things right!

FC Monson - THE STUDENTS ARE BACK!!!! Is there not a song with words like,
"pre-meds every the morning, and pre-meds every night, ..."?

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center, Room SS024
West Chester University of Pennsylvania
South Church Street & Rosedale Ave.
West Chester, PA, 19383
Phone & FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/


-----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
Sent: Monday, August 19, 2002 1:06 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938


From daemon Wed Aug 21 04:08:42 2002



From: earlw-at-sbcglobal.net :      earlw-at-sbcglobal.net
Date: Tue, 20 Aug 2002 21:48:18 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is clearly (what we call in the States) "Too much information."

Earl


Original Message:
-----------------
} From: Monson, Frederick C. fmonson-at-wcupa.edu


And I, I of the old school, use my genuine, official, AO-Polaroid, WWII,
flying-ace, flight glasses. Whenever the C-sun gets brighter, I just turn
the knob and cross my polarizers. And, they were free!!!!

For the students, there is nothing so flashy. They get good, pre-tested,
welders goggles, but there is nothing like crossed and crosser polarizers.

Unfortunately, I've lost the optics from my submarine periscope and I can't
use my goggles from around the corner of the room. I am now thinking of the
old wooden box with two mirrors trick.

As for self-discipline, I lost that sometime during my wedding night, but I
have been searching for it ever since. And, like so many [old(?)] men after
35+ years of marriage, I hurry home each night to buss the wife and smile my
secret smiles - still clueless about it all. And while the Sry gene finally
gives some meaning to the existence of the Y chromosome, we who have been
thus pre-determined feel short-changed without the ability to choose, that
having been taken from us by an arbitrary sequence of nucleotides for which
we neither asked nor were given the power or right to exorcise. Yet, still,
I humbly count my blessings. I could have been born a Medaca, with two Y's,
and still been twice oppositely determined, in an estrogenized aquarium, to
carry XX plumbing. Would that have been an example of one unfulfilled
expectation, or would that count as two?

"And now", she says, "he feels content, having new company in his clueless
abyss where no one knows what makes a man - or a woman". And, if your baby
Medacas are born with "incorrect" chromosomes, you may merely have to spike
the water to set things right!

FC Monson - THE STUDENTS ARE BACK!!!! Is there not a song with words like,
"pre-meds every the morning, and pre-meds every night, ..."?

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center, Room SS024
West Chester University of Pennsylvania
South Church Street & Rosedale Ave.
West Chester, PA, 19383
Phone & FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/


-----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
Sent: Monday, August 19, 2002 1:06 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938


--------------------------------------------------------------------
mail2web - Check your email from the web at
http://mail2web.com/ .




From daemon Wed Aug 21 07:39:48 2002



From: =?iso-8859-1?q?Jeremy=20Sanderson?= :      jb_sanderson-at-yahoo.com
Date: Wed, 21 Aug 2002 13:30:23 +0100 (BST)
Subject: Re: Eye Protection and Other Frustra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr Monson,

Your recent post has appalled me, and I suspect many
others; Earl Weltmer let you down kindly and
diplomatically. I routinely delete your posts which
clog up my mailbox. Sorry, but you need to hear this.

Yours

Jeremy Sanderson



__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com


From daemon Wed Aug 21 09:22:04 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 21 Aug 2002 09:13:33 -0500
Subject: TEM/LM: Problems with cat retinal tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

We are having some difficulty with getting good thick sections from feline retinal tissue. The sections look somewhat uneven in thickness (wavy), and in the nerve fiber layer and at the level of the inner limiting membrane there is vacuolization. There are also tears in some areas. Our standard processing procedures use 2% gluteraldehyde/2% paraformaldehyde in 0.1M cacodylate buffer, 1% osmium post-fix, 1% uranyl acetate en bloc stain, ethanol dehydration, and Spurr's/EPON resin. We are currently trying 2.5% gluteraldehyde in cacodylate (at 0.1 and 0.17M), as well as combining microwave fixation with our standard procedures.

Our current procedures---microwave and otherwise---work very well with mouse retinal tissue, but cats are being contrary. If anyone has any tried and true techniques, I'd be very grateful to hear about them. It could save us tons of time and frustration as we develop a new set of protocols.

Thanks very much.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Wed Aug 21 13:04:39 2002



From: JHoffpa464-at-aol.com
Date: Wed, 21 Aug 2002 13:53:41 -0400
Subject: Re: Eye Protection and Other Frustra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


another stick in the mud. seems like a direct attack, should he be banned from the list server?



From daemon Wed Aug 21 13:04:39 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 21 Aug 2002 11:07:15 -0700
Subject: Re: Installation of old detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Earl Weltmer" {earlw-at-sbcglobal.net}
To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com} ; "Monson, Frederick C."
{fmonson-at-wcupa.edu} ; {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 21, 2002 10:46 AM


Dear Mr. Ding,
As your old detector has been sitting without liquid nitrogen for several
months, it must be considered to have been thermally cycled, that is, warmed
up, at least once. For the health of the window, the best sequence is:
1. install the detector on the microscope, which is vented.
2. Fill the dewar with liquid nitrogen. This will increase the vacuum in the
dewar, so there will be no chance of pressure on the wrong side of the window.
3. Pump down the SEM.
4. After four hours of liquid nitrogen fill, connect the cables from the
analyser, then turn on the bias voltage to the detector. Wait at least
one-half hour for the system to stabilize before taking spectra.
5. Calibrate the detector. You may have to find the coarse and fine pots to
match the old detector to the analyser.
The time your detector was not cold may have caused some degradation in the
resolution or peak tailing, but maybe not too much.
At 11:50 AM 08/20/2002 -0400, you wrote:
}
} Dear Listers:
}
} Our new detector has been damaged. I will try to install our
} old detector in JMS-840 SEM for a temporary use, before I can get a new
} detector. This old detector (Link Pentafet plus detector) was used in the
} same SEM around one and half years ago. Parameters of the old detector are
} given in the following:
}
} Model: 6276
} Det. Area: 10.0 mmxmm
} Window: 0.008
} Resol.: 137 ev at 5.99 ekv
} Serial: 1080-3929
} Att. No.: 32cc32088
}
} The manual shows the "EXCHANGE DETECTOR" procedure. But this procedure
} does not indicate details on filling liquid nitrogen. If anybody of you has
} experience with installation of this type of detectors, I would like to have
} your opinions and thoughts. This detector has not used for one and half
} years. Also I have some questions on installation of the detector as
} follows.
}
} 1) When liquid nitrogen will be filled? I think that first the detector
} assembly will be fixed in the SEM, and then liquid nitrogen will be filled
} until the chamber vacuum is ready.
} 2) I don't know if the old detector can be thermally cycled. What procedure
} (warm up & cool down?) will be performed during filling liquid nitrogen? Or
} an easy way is just to fill liquid nitrogen, then and wait for the
} next calibration after four hours ?
}
} Any suggestions and recommendation on installation of this old detector will
} be greatly appreciated. Thank you for your help.
}
} Yuquan Ding
} Dept of Mechanical Engineering
} University of Waterloo
} Waterloo, ON Canada N2L 3G1
} Tel: 519-888-4567 ext 3766
}
Regards and good luck!
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Aug 21 13:21:39 2002



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Wed, 21 Aug 2002 13:14:19 -0500
Subject: Adding a digital camera to an optical microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm
looking to add a digital camera to and I have gotten rather confused by all
the choices out there. I could use some advice regarding what are the
important things to look for.

Recently I have been making more use of this microscope and have been
dissatisfied with the old Polaroid camera attachment used to take pictures
of the samples. I would like to junk that and replace it with a PC based
color digital camera. I still don't foresee heavy use for this microscope,
but my needs (hair images) are more demanding than the previous rather
casual use we made of this guy.

I have seen a number of choices up at the M&M show and am collecting
literature and quotes. But I wonder about whether attaching a higher end
consumer camera (I have literature for a Kodak DC290, for example) will be
good enough or if I should spend more money on a more specialized camera,
like a Spot Insight that a local vendor has demonstrated for me (seemed nice
enough). What is important and what not?

And where does one get a C mount since we don't have one and the cameras
don't necessarily seem to come with one.

Many thanks as always.

Richard Shalvoy
Arch Chemicals, Inc.
350 Knotter Drive
Cheshire, CT 06410
(203) 271-4394
rbshalvoy-at-archchemicals.com




From daemon Wed Aug 21 15:33:44 2002



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Wed, 21 Aug 2002 21:20:26 +0100
Subject: Re: TEM: Lorentz microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

It has been a long time but, if I remember correctly (which I would
not guarantee), I think that you can only do Lorentz microscopy in a
standard CM in the low mag mode, when the objective is "off" (it
actually has a small current for aberation correction).
Unfortunately, this limits the maximum magnifaction to ~x3,000.

In the main mag modes, the objective lens field is on and much too
strong, causing the specimens to completely saturate (and sometimes
pulling them out of the holder). You will also require a 'small'
objective aperture to generate Lorentz contrast - however, in the LM
mode, this means a medium aperture by comparision with those normally
used in the main mag ranges - 50 micron?

Theer are a few (3?) CMs around with special 'Lorentz' objectives,
allowing imaging of magnetic domains at much higher magnifications
(x30,000).

I must declare an interest, currently being an employee of JEOL UK.
--
Larry Stoter


From daemon Wed Aug 21 16:42:30 2002



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 21 Aug 2002 14:29:26 -0700 (PDT)
Subject: sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I've got a clay in a resin I would like to section, but ordinary water
won't work. As soon as the clay hits water, it disperses and comes out of
the resin. I used to make light microscope thin sections using kerosene
for clays like these instead of water. Is there another fluid I could
use for sectioning the samples other than water? Kerosene just jumps to
the block and wets the whole face.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Wed Aug 21 17:57:56 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 21 Aug 2002 16:45:11 -0600
Subject: Adding a digital camera to an optical microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard,

I will send you information about our cameras by mail, but I wanted to
address a couple of the points you are raising here:

1) You mention the Kodak DC290. That camera is actually discontinued.

2) If you purchase a camera like the Kodak DC290, make sure that you can get
uncompressed images into your computer. Most cameras compress the images
using a JPG algorithm, which introduces artifacts. Depending on what you do,
these artifacts may be severe enough to prevent further working with the
images.

3) Live viewing with this type of camera may be restricted to the LCD
monitor on the camera (or viewfinder), which is normally too small to do
anything.

4) Some of the cheaper cameras may use a cheap lens, potentially adding to
distortion and reducing resolution.

5) You need a special holder for these cameras, as they usually don't adhere
to any standards.

6) I have never seen any specifications about the sensitivity and dynamic
range of the consumer cameras. I suppose it's not that great.

7) C-mount: Most scientific cameras come with a C-mount, which is really
nothing more thrilling than a certain thread and some geometric
considerations on the camera side. If you want to connect this to your
microscope, you need a c-mount adapter, which you can get from the
microscope manufacturer. C-mount adapters come in different flavors, with
different lenses. Depending on the chip-size of the camera you need a
specific c-mount if you want to keep the field of view the same through
binoculars and camera.

8) Resolution: There is a wide variety of scientific cameras with different
resolutions. In general you need the higher resolution for a larger field of
view. For example, an image taken on a 1300x1024 camera at 1000x is probably
not worse than one taken with a 200x1500 camera, as the resolution is
determined by the N.A. of the lens. On the other hand, at a lower
magnification, the larger chip can show more details for the same field of
view.

I hope I have not confused you more with this information. Give me a call or
drop me an email if you have any questions.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Shalvoy, Richard B **CHES [mailto:RBShalvoy-at-archchemicals.com]
Sent: Wednesday, August 21, 2002 12:14 PM
To: Microscopy-at-sparc5.microscopy.com


I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm
looking to add a digital camera to and I have gotten rather confused by all
the choices out there. I could use some advice regarding what are the
important things to look for.

Recently I have been making more use of this microscope and have been
dissatisfied with the old Polaroid camera attachment used to take pictures
of the samples. I would like to junk that and replace it with a PC based
color digital camera. I still don't foresee heavy use for this microscope,
but my needs (hair images) are more demanding than the previous rather
casual use we made of this guy.

I have seen a number of choices up at the M&M show and am collecting
literature and quotes. But I wonder about whether attaching a higher end
consumer camera (I have literature for a Kodak DC290, for example) will be
good enough or if I should spend more money on a more specialized camera,
like a Spot Insight that a local vendor has demonstrated for me (seemed nice
enough). What is important and what not?

And where does one get a C mount since we don't have one and the cameras
don't necessarily seem to come with one.

Many thanks as always.

Richard Shalvoy
Arch Chemicals, Inc.
350 Knotter Drive
Cheshire, CT 06410
(203) 271-4394
rbshalvoy-at-archchemicals.com




From daemon Wed Aug 21 18:34:40 2002



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Tue, 20 Aug 2002 21:48:18 -0400
Subject: Administrivia: June 2002 Archives are now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well, I've always been fascinated by what human society would be like if we behaved like coral trout. Would one gender treat the other with infinite understanding, or would we follow a pattern like school yard bullying and boot camp hazing? Opens up a huge range of possibilities in between, anyway.

Dont know how you find the time Fred, but I always enjoy your posts.

Sally Stowe


} } } "earlw-at-sbcglobal.net" {earlw-at-sbcglobal.net} 08/21/02 07:00PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


This is clearly (what we call in the States) "Too much information."

Earl


Original Message:
-----------------
} From: Monson, Frederick C. fmonson-at-wcupa.edu


And I, I of the old school, use my genuine, official, AO-Polaroid, WWII,
flying-ace, flight glasses. Whenever the C-sun gets brighter, I just turn
the knob and cross my polarizers. And, they were free!!!!

For the students, there is nothing so flashy. They get good, pre-tested,
welders goggles, but there is nothing like crossed and crosser polarizers.

Unfortunately, I've lost the optics from my submarine periscope and I can't
use my goggles from around the corner of the room. I am now thinking of the
old wooden box with two mirrors trick.

As for self-discipline, I lost that sometime during my wedding night, but I
have been searching for it ever since. And, like so many [old(?)] men after
35+ years of marriage, I hurry home each night to buss the wife and smile my
secret smiles - still clueless about it all. And while the Sry gene finally
gives some meaning to the existence of the Y chromosome, we who have been
thus pre-determined feel short-changed without the ability to choose, that
having been taken from us by an arbitrary sequence of nucleotides for which
we neither asked nor were given the power or right to exorcise. Yet, still,
I humbly count my blessings. I could have been born a Medaca, with two Y's,
and still been twice oppositely determined, in an estrogenized aquarium, to
carry XX plumbing. Would that have been an example of one unfulfilled
expectation, or would that count as two?

"And now", she says, "he feels content, having new company in his clueless
abyss where no one knows what makes a man - or a woman". And, if your baby
Medacas are born with "incorrect" chromosomes, you may merely have to spike
the water to set things right!

FC Monson - THE STUDENTS ARE BACK!!!! Is there not a song with words like,
"pre-meds every the morning, and pre-meds every night, ..."?

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center, Room SS024
West Chester University of Pennsylvania
South Church Street & Rosedale Ave.
West Chester, PA, 19383
Phone & FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/


-----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
Sent: Monday, August 19, 2002 1:06 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

For many years, I've used nothing but self-discipline to avoid direct eye
contact with the white-hot electrodes resulting during a carbon evaporation
run. However, times have changed and now I am responsible for the safety of
student users.

Does anyone know whether an arc-welder's face-shield would protects the eyes
from this type of intense light? My impression is that the face-shield
mainly protects the welder from emitted sparks, and I don't know whether the
light emission is similar to that from carbon evaporation.

If not, what eye protection are others using out there in List-land?

Thanks as always for your help.

Ann

###################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/
Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm


-----Original Message-----
} From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr]
Sent: Monday, August 19, 2002 9:22 AM
To: Microscopy-at-sparc5.microscopy.com


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues...

The June 2002 archives of the Microscopy Listserver are now on-line.

at http://www.msa.microscopy.com/MicroscopyListserver


Cheers...

Nestor
Your Friendly Neighborhood SysOp

Dr Jean-Hervé Lignot,
Université Louis Pasteur / CNRS CEPE
23 rue Becquerel, 67087 Strasbourg, Cedex 2, France
Fax: 00 33 0388106906
Tel: 00 33 0388106938


--------------------------------------------------------------------
mail2web - Check your email from the web at
http://mail2web.com/ .





From daemon Wed Aug 21 21:25:26 2002



From: rcmoretz-at-att.net
Date: Thu, 22 Aug 2002 02:14:38 +0000
Subject: Re: TEM/LM: Problems with cat retinal tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy:
It has been a long time since I have done cat retina,
but there were are few papers along the way. The only
real difference I see in protocols (I don't think the
glut/para vs glut is a problem--I generally use 2%pf +
1% glut, but have used straight glut as well) is that I
have stayed with straight Epon (usually EMBED 812 from
EM Sciences, but others have worked equally well) with
little or no tearing or wrinkling. As for the
vacuolization, are these eyes perfused or immersion
fixed? My experience (from rats to cats to dogs to
primates) is that vacuolization generally occurs during
removal of the eye (due to too much force being applied
or too much pulling) or the enucleation process.
Another variable can be the trimming of the samples for
EM--i.e. using a crushing cut rather than a slicing
cut. Don't know if any of these are your problems.
Just things I have had to work out over the years.

Roger Moretz
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} We are having some difficulty with getting good thick sections from feline
} retinal tissue. The sections look somewhat uneven in thickness (wavy), and in
} the nerve fiber layer and at the level of the inner limiting membrane there is
} vacuolization. There are also tears in some areas. Our standard processing
} procedures use 2% gluteraldehyde/2% paraformaldehyde in 0.1M cacodylate buffer,
} 1% osmium post-fix, 1% uranyl acetate en bloc stain, ethanol dehydration, and
} Spurr's/EPON resin. We are currently trying 2.5% gluteraldehyde in cacodylate
} (at 0.1 and 0.17M), as well as combining microwave fixation with our standard } procedures.
}
} Our current procedures---microwave and otherwise---work very well with mouse
} retinal tissue, but cats are being contrary. If anyone has any tried and true
} techniques, I'd be very grateful to hear about them. It could save us tons of
} time and frustration as we develop a new set of protocols.
}
} Thanks very much.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}


From daemon Wed Aug 21 22:09:29 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 21 Aug 2002 20:03:39 -0700
Subject: RE: Eye Protection and Other Frustra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think that Fred deserves another open opportunity to post.

This was not information overload. Rather, it was a
dearth of information, as I see it. I think that he was caught at
a bad time. S.... happens. Am I wrong or am I wright?

Only he knows.

gary g.




At 04:25 PM 8/21/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Aug 21 23:33:34 2002



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Thu, 22 Aug 2002 14:28:13 +1000
Subject: TEM/LM: Problems with cat retinal tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Randy,

I've embedded some cat retina, using my "basic" method, with fine
results. The user fixes (I like cats too much!) - sometimes glut
(2.5% in 0.1M cacodylate pH 7.4), sometimes Karnovsky's, immersion or
infused. Then 2% Os 2 hrs, UAc 1hr, EtOH 10-15 mins each, 2x acetone
15 min each, Epon/Araldite embed. I know with human retina that too
much vitreous really messes up embedding - could that be a problem
with cats?

Diana


From daemon Thu Aug 22 02:59:02 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 22 Aug 2002 00:47:36 -0400
Subject: Re: Eye protection during C-evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 8/19/02 1:05 PM, Lehman, Ann at Ann.Lehman-at-trincoll.edu wrote:
}
} For many years, I've used nothing but self-discipline to avoid direct eye
} contact with the white-hot electrodes resulting during a carbon evaporation
} run. However, times have changed and now I am responsible for the safety of
} student users.
}
} Does anyone know whether an arc-welder's face-shield would protects the eyes
} from this type of intense light? My impression is that the face-shield
} mainly protects the welder from emitted sparks, and I don't know whether the
} light emission is similar to that from carbon evaporation.
}
} If not, what eye protection are others using out there in List-land?
}
} Thanks as always for your help.
}
Dear Ann,
Even the most trouble-prone student is very unlikely to have an eye in
direct contact with a white-hot electrode ;-). Welding goggles or a face
shield will protect against the intense light. The light from the carbon
rods and that from a welding arc are sufficiently similar that the welders'
eye protection will work well. The welding arc does have more UV than the
carbon rods--especially as the latter are inside a glass bell jar and
plastic implosion shield. Now all you need to do is to be sure that the
students actually wear the gear and lower the shield when they start up the
current. Good luck.
Yours,
Bill Tivol



From daemon Thu Aug 22 02:59:07 2002



From: Dr. Peter Heimann :      peter.heimann-at-uni-bielefeld.de
Date: Thu, 22 Aug 2002 09:52:30 +0200
Subject: Isolation method for HRP from native material ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,
is anybody aware of a method to purify active HRP (horseradish
peroxidase)from native tissue (e.g. horseradish). High purity of isolate
would not be necessary.
Thanks for any suggestion,
Peter Heimann
**********************************
peter.heimann-at-uni-bielefeld.de
Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : " " - 5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie/
WEB-Site: http://www.uni-bielefeld.de/SFB549
***********************************



From daemon Thu Aug 22 08:02:58 2002



From: Susan Carbyn :      CarbynS-at-agr.gc.ca
Date: Thu, 22 Aug 2002 08:51:37 -0400
Subject: Re: Adding a digital camera to an optical microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Richard,

We have a digital camera attached to our light microscope with a coupler purchased from Thales-Optem in Fairport, NY. Their phone number is 716-223-2370 x 178 and the person to talk with about your questions is Patrick Harrington. I found him to be extremely helpful with my questions, and he even suggested a Canadian supplier as well, for a C mount for our dissecting scope.

Hope this helps,

Susan

Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, N.S. B4N 1J5
Canada

Phone (902) 679-5535
Fax (902) 679-2311

E-Mail: carbyns-at-em.agr.ca

} } } "Shalvoy, Richard B **CHES" {RBShalvoy-at-archchemicals.com} 08/21/02 03:14PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm
looking to add a digital camera to and I have gotten rather confused by all
the choices out there. I could use some advice regarding what are the
important things to look for.

Recently I have been making more use of this microscope and have been
dissatisfied with the old Polaroid camera attachment used to take pictures
of the samples. I would like to junk that and replace it with a PC based
color digital camera. I still don't foresee heavy use for this microscope,
but my needs (hair images) are more demanding than the previous rather
casual use we made of this guy.

I have seen a number of choices up at the M&M show and am collecting
literature and quotes. But I wonder about whether attaching a higher end
consumer camera (I have literature for a Kodak DC290, for example) will be
good enough or if I should spend more money on a more specialized camera,
like a Spot Insight that a local vendor has demonstrated for me (seemed nice
enough). What is important and what not?

And where does one get a C mount since we don't have one and the cameras
don't necessarily seem to come with one.

Many thanks as always.

Richard Shalvoy
Arch Chemicals, Inc.
350 Knotter Drive
Cheshire, CT 06410
(203) 271-4394
rbshalvoy-at-archchemicals.com






From daemon Thu Aug 22 09:43:16 2002



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Thu, 22 Aug 2002 14:04:34 -0400
Subject: Re: Adding a digital camera to an optical microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl;

I'd be happy to solicit loans and grants from the folks on the listserver.
Albeit I'm in New Jersey and not Nigeria but I can do a pretty good fake
Nigerian accent, especially if money is involved.

I think folks need to lighten up and I doubt whether Fred intended to be
insulting to any species. As a married man with kids I know that humor is
the best remedy at times for things that ail us. I can start a listserver
just on my own domestic frustra.

Peter

-----Original Message-----
} From: Earl Weltmer [mailto:earlw-at-sbcglobal.net]
Sent: Wednesday, August 21, 2002 1:56 PM
To: Microscopy-at-sparc5.microscopy.com



----- Original Message -----
} From: "Earl Weltmer" {earlw-at-sbcglobal.net}
To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com} ; "Monson, Frederick C."
{fmonson-at-wcupa.edu} ; {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 21, 2002 10:46 AM


http://www.unwords.com/sniglets/frustra.shtml

----- Original Message -----
} From: Peter Tomic {PTomic-at-anadigics.com}


Area code for this 716 area is now 585

-----Original Message-----
} From: Susan Carbyn [mailto:CarbynS-at-agr.gc.ca]
Sent: Thursday, August 22, 2002 8:52 AM
To: Microscopy-at-sparc5.microscopy.com


Hi Richard,

We have a digital camera attached to our light microscope with a coupler
purchased from Thales-Optem in Fairport, NY. Their phone number is
716-223-2370 x 178 and the person to talk with about your questions is
Patrick Harrington. I found him to be extremely helpful with my questions,
and he even suggested a Canadian supplier as well, for a C mount for our
dissecting scope.

Hope this helps,

Susan

Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, N.S. B4N 1J5
Canada

Phone (902) 679-5535
Fax (902) 679-2311

E-Mail: carbyns-at-em.agr.ca

} } } "Shalvoy, Richard B **CHES" {RBShalvoy-at-archchemicals.com} 08/21/02
03:14PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm
looking to add a digital camera to and I have gotten rather confused by all
the choices out there. I could use some advice regarding what are the
important things to look for.

Recently I have been making more use of this microscope and have been
dissatisfied with the old Polaroid camera attachment used to take pictures
of the samples. I would like to junk that and replace it with a PC based
color digital camera. I still don't foresee heavy use for this microscope,
but my needs (hair images) are more demanding than the previous rather
casual use we made of this guy.

I have seen a number of choices up at the M&M show and am collecting
literature and quotes. But I wonder about whether attaching a higher end
consumer camera (I have literature for a Kodak DC290, for example) will be
good enough or if I should spend more money on a more specialized camera,
like a Spot Insight that a local vendor has demonstrated for me (seemed nice
enough). What is important and what not?

And where does one get a C mount since we don't have one and the cameras
don't necessarily seem to come with one.

Many thanks as always.

Richard Shalvoy
Arch Chemicals, Inc.
350 Knotter Drive
Cheshire, CT 06410
(203) 271-4394
rbshalvoy-at-archchemicals.com






From daemon Thu Aug 22 13:38:28 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 22 Aug 2002 14:29:16 -0400
Subject: Technicon Processors - Parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't know how, but a previous was sent in rich rather than plain text.
My apology.

The following URL is for a company that claims to produce new and have parts
for Technicon processors. I have never contacted this company, but the URL
has come up several times in searches on the net. For those who are
interested, here it is.

http://www.datacut.com/gg/products.htm

Hope this helps some,

FCM

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center, Room SS024
West Chester University of Pennsylvania
South Church Street & Rosedale Ave.
West Chester, PA, 19383
Phone & FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/


From daemon Thu Aug 22 17:29:27 2002



From: Boron nitride :      boronnitride-at-hotmail.com
Date: Thu, 22 Aug 2002 16:22:33 -0600
Subject: Quote for GIF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please specify which email that was sent in rich instead of plain text: the
one about your wife or the "Technicon Processor".

Earl Weltmer


----- Original Message -----
} From: "Monson, Frederick C." {fmonson-at-wcupa.edu}
To: "List-HistoPath (E-mail)" {histonet-at-pathology.swmed.edu} ;
"List-Microscopy (E-mail)" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, August 22, 2002 11:29 AM


Hello, Fellows,

We are planning to buy the GIF for our new JEOL 2010F, could somebody let us
know what is the reasonable cost to buy this system?

Thanks a lot!

James



_________________________________________________________________
Chat with friends online, try MSN Messenger: http://messenger.msn.com



From daemon Thu Aug 22 18:08:50 2002



From: Boron nitride :      boronnitride-at-hotmail.com (by way of
Date: Thu, 22 Aug 2002 17:58:48 -0500
Subject: Price of GIF 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Does anybody know the cost to buy GIF 2000 (Gatan Image Filter) system?

Thanks a lot!

James


_________________________________________________________________
MSN Photos is the easiest way to share and print your photos:
http://photos.msn.com/support/worldwide.aspx


From daemon Thu Aug 22 20:40:23 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 22 Aug 2002 21:30:17 -0700
Subject: RE: RE: Eye Protection and Other Frustra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you just let the bell jar get good and dirty, and never clean it, after a
while nobody will be able to see anything inside the bell jar, and you won't
need goggles or anything.

John Mardinly
Intel



-----Original Message-----
} From: "MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com
[mailto:"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com]
Sent: Thursday, August 22, 2002 8:53 AM
To: Peter Tomic
Cc: 'Earl Weltmer'; Microscopy-at-sparc5.microscopy.com


http://www.unwords.com/sniglets/frustra.shtml

----- Original Message -----
} From: Peter Tomic {PTomic-at-anadigics.com}


I'm not sure that I would agree with this. If the coating
was very thick, that might be OK. But we are dealing
with both brightness and UV. How thick of a coating is
needed to prevent UV damaging eye exposure? Who
knows?

I would not risk it. A $75 hood is well worth my eyes
for my lifetime. Who suffers during the build up of
coating? Not me. Will you?

gary g.


At 06:32 PM 8/22/2002, you wrote:

} If you just let the bell jar get good and dirty, and never clean it, after a
} while nobody will be able to see anything inside the bell jar, and you won't
} need goggles or anything.
}
} John Mardinly
} Intel
}
}
}
} -----Original Message-----
} } From: "MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com
} [mailto:"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com]
} Sent: Thursday, August 22, 2002 8:53 AM
} To: Peter Tomic
} Cc: 'Earl Weltmer'; Microscopy-at-sparc5.microscopy.com
} Subject: Re: RE: Eye Protection and Other Frustra
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Aug 23 00:57:39 2002



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Fri, 23 Aug 2002 15:54:42 +1000
Subject: Re: Eye Protection and Other Frustra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,
UV is adsorbed by fairly thin plastic and unless the glass belljar is
made of quartz no UV will penetrate through it.

JVN

Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm not sure that I would agree with this. If the coating
} was very thick, that might be OK. But we are dealing
} with both brightness and UV. How thick of a coating is
} needed to prevent UV damaging eye exposure? Who
} knows?
}
} I would not risk it. A $75 hood is well worth my eyes
} for my lifetime. Who suffers during the build up of
} coating? Not me. Will you?
}
} gary g.
}
}
} At 06:32 PM 8/22/2002, you wrote:
}
} } If you just let the bell jar get good and dirty, and never clean it,
} } after a
} } while nobody will be able to see anything inside the bell jar, and you
} } won't
} } need goggles or anything.
} }
} } John Mardinly
} } Intel
} }
} }
} }
} } -----Original Message-----
} } } From: "MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com
} } [mailto:"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com]
} } Sent: Thursday, August 22, 2002 8:53 AM
} } To: Peter Tomic
} } Cc: 'Earl Weltmer'; Microscopy-at-sparc5.microscopy.com
} } Subject: Re: RE: Eye Protection and Other Frustra
} }
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } http://www.unwords.com/sniglets/frustra.shtml
} }
} } ----- Original Message -----
} } } From: Peter Tomic {PTomic-at-anadigics.com}
} } Date: Thursday, August 22, 2002 7:40 am
} } Subject: RE: Eye Protection and Other Frustra
} }
} } } -------------------------------------------------------------------
} } } -----
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } AmericaTo Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.ComOn-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html--------
} } } ---------------------------------------------------------------.
} } }
} } }
} } } Earl;
} } }
} } } I'd be happy to solicit loans and grants from the folks on the
} } } listserver.Albeit I'm in New Jersey and not Nigeria but I can do a
} } } pretty good fake
} } } Nigerian accent, especially if money is involved.
} } }
} } } I think folks need to lighten up and I doubt whether Fred intended
} } } to be
} } } insulting to any species. As a married man with kids I know that
} } } humor is
} } } the best remedy at times for things that ail us. I can start a
} } } listserverjust on my own domestic frustra.
} } }
} } } Peter
} } }
} } } -----Original Message-----
} } } } From: Earl Weltmer [mailto:earlw-at-sbcglobal.net]
} } } Sent: Wednesday, August 21, 2002 1:56 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: Fw: Eye Protection and Other Frustra
} } }
} } }
} } } -------------------------------------------------------------------
} } } -----
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } AmericaTo Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.ComOn-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html--------
} } } ---------------------------------------------------------------.
} } }
} } }
} } }
} } } ----- Original Message -----
} } } } From: "Earl Weltmer" {earlw-at-sbcglobal.net}
} } } To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com} ; "Monson,
} } } Frederick C."
} } } {fmonson-at-wcupa.edu} ; {microscopy-at-sparc5.microscopy.com}
} } } Sent: Wednesday, August 21, 2002 10:46 AM
} } } Subject: Re: Eye Protection and Other Frustra
} } }
} } }
} } } } Well, I wasn't appalled but humored which we all need from time
} } } to time.
} } } }
} } } } Which reminds me, it seem that the List has been rather
} } } "listless" of
} } } late.
} } } }
} } } } It seem that we don't get the numbers nor the level of info that I
} } } normally
} } } } receive.
} } } }
} } } } Is it real or just my perception?
} } } }
} } } } I was even looking forward to another letter from Nigeria asking
} } } for my
} } } bank
} } } } account number.
} } } } By the way, I have received so many "Nigerian Letters" that I
} } } have been
} } } } forwarding them to each other. Hopefully they can con each other.
} } } }
} } } } Maybe that "Businessman named Helen from Dallas" can use their help.
} } } }
} } } }
} } } } Yes I do have a life but I look forward to the Listserver info
} } } as well.
} } } }
} } } } Best Regards All,
} } } }
} } } }
} } } } Earl
} } } }
} } } } ----- Original Message -----
} } } } From: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com}
} } } } To: "Monson, Frederick C." {fmonson-at-wcupa.edu} ;
} } } } {microscopy-at-sparc5.microscopy.com}
} } } } Sent: Wednesday, August 21, 2002 5:30 AM
} } } } Subject: Re: Eye Protection and Other Frustra
} } } }
} } } }
} } } } } ---------------------------------------------------------------
} } } ---------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } } of America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com} } On-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } ----
} } } -------------------------------------------------------------------.
} } } } }
} } } } }
} } } } } Dear Mr Monson,
} } } } }
} } } } } Your recent post has appalled me, and I suspect many
} } } } } others; Earl Weltmer let you down kindly and
} } } } } diplomatically. I routinely delete your posts which
} } } } } clog up my mailbox. Sorry, but you need to hear this.
} } } } }
} } } } } Yours
} } } } }
} } } } } Jeremy Sanderson
} } } } }
} } } } }
} } } } }
} } } } } __________________________________________________
} } }
} } }
} } } } }
} } } } }
} } } }
} } }
} } }
} } }
}
}
}
}
}


--
John V Nailon
Executive Officer and Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld



From daemon Fri Aug 23 07:53:48 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Fri, 23 Aug 2002 08:44:11 -0400
Subject: RE: Adding a digital camera to a[n] [visible light] optical microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Check out http://www.thales-optem.com and their digital camera adapters.

I think that is the route we are going to take as an additional back up
for a couple of our microscopes. We have a few Nikon 990 3.34MP digital
cameras in the department and for the $325 they ask for the ocular tube
adapter is down right affordable.

And BTW - don't you mean 'Light' microscope. Most microscopes electron
and visible wave length are "Optical" :D ;) (sorry couldn't help it)

Geoff Williams
Microscopy Facility Supervisor

Checkout the new Biology Department Microscopy Facility web page.
Version 1 is now On-Line:
www.cst.cmich.edu/users/willi1gl/BDMF/BDMF.htm

} -----Original Message-----
} From: Shalvoy, Richard B **CHES [mailto:RBShalvoy-at-archchemicals.com]
} Sent: Wednesday, August 21, 2002 2:14 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Adding a digital camera to an optical microscope
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} I have a lightly used 10 year old Nikon Optiphot-POL microscope that
I'm
} looking to add a digital camera to and I have gotten rather confused
by
} all
} the choices out there. I could use some advice regarding what are the
} important things to look for.
}
} Recently I have been making more use of this microscope and have been
} dissatisfied with the old Polaroid camera attachment used to take
pictures
} of the samples. I would like to junk that and replace it with a PC
based
} color digital camera. I still don't foresee heavy use for this
} microscope,
} but my needs (hair images) are more demanding than the previous rather
} casual use we made of this guy.
}
} I have seen a number of choices up at the M&M show and am collecting
} literature and quotes. But I wonder about whether attaching a higher
end
} consumer camera (I have literature for a Kodak DC290, for example)
will be
} good enough or if I should spend more money on a more specialized
camera,
} like a Spot Insight that a local vendor has demonstrated for me
(seemed
} nice
} enough). What is important and what not?
}
} And where does one get a C mount since we don't have one and the
cameras
} don't necessarily seem to come with one.
}
} Many thanks as always.
}
} Richard Shalvoy
} Arch Chemicals, Inc.
} 350 Knotter Drive
} Cheshire, CT 06410
} (203) 271-4394
} rbshalvoy-at-archchemicals.com
}




From daemon Fri Aug 23 08:46:04 2002



From: Kuusisto, Ari :      Ari.Kuusisto-at-PerkinElmer.com
Date: Fri, 23 Aug 2002 16:35:54 +0300
Subject: Tetenal camera varnish 100912

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am sending this for a colleague of mine. He would be grateful if somebody
can help him.

'Tetenal has been discontinued their camera varnish 100912. Does anyone know
something similar varnish for gluing optical components?

__________________________________________________________
Mr Raimo Harju, M.Sc.,
Technology Lead, Photonics
Instrument Development
PerkinElmer Life Sciences
Wallac Oy
P.O.Box 10, FIN-20101 Turku, Finland
Phone: +358 2 2678111, Direct: +358 2 2678224, Fax: +358 2 2678530
Mobile: +358 40 7171629
E-mail: raimo.harju-at-perkinelmer.com,
Website:www.perkinelmer.com'


Ari Kuusisto, Research Physicist

PerkinElmer Life Sciences,
Wallac Oy

Phone: +358 2 267 8508 (direct)
+358 2 267 8111 (switchboard)
Fax: +358 2 267 8357
e-mail: Ari.Kuusisto-at-PerkinElmer.com
Ari.Kuusisto-at-Wallac.fi
Mail address: PO Box 10
FIN-20101 Turku,
Finland.

Street address: Laituri 4
Mustionkatu 6,
FIN-20750 TURKU
Finland





From daemon Fri Aug 23 11:27:52 2002



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Fri, 23 Aug 2002 12:19:22 -0400
Subject: RE: TEM/LM: Problems with cat retinal tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,

I have been working with rabbit retina that has been lased at different
pulses/pulse durations, looking for damage in the epithelial layer. I have
had excellent preservation of the retina (the normal adjacent areas as well
as the damaged areas). I recently purchased a microwave to do fixation and
processing, and ran a couple of duplicate samples to compare. I also got
good results.

Because I haven't found a really inclusive protocol for microwave processing
(I couldn't get my blocks to polymerize via the microwave), I resorted to
using a conventional oven for that stage.

THE PROTOCOL:

1. The eyes were injected with Karnovsky's Fixative( usually, sometimes
with 4% glut) after lasing and left overnight in the cold (this is to
maintain the integrity of the structures).

2. The posterior segment of the eye is cut away and then put in fresh 4%
Glutaraldehyde in 0.1M Cacodylate Buffer (pH 7.4) and stored in the cold.
Note: these segments can remain in the cold indefinitely (i.e. two weeks).

3. The vitreous fluid is teased away gently so as not to disrupt the
underlying retinal structures. The lased areas are dissected away from rest
of posterior segment using an ophthalmological knife*. Again, to avoid
disrupting the structures.

4. The lased segments (-at-1mm square) are put into 0.1M Cacodylate Buffer (pH
7.4) until processing.

Processing:

5. 3 X 15 minutes - 0.1M Cacodylate Buffer
6. Overnight fixation in 2% OsO4 in cacodylate buffer (in the cold)
7. 2 X 15 minutes - 0.1M Cacodylate Buffer
8. Dehydration: 25% ETOH, 50% ETOH (2 X 15 minute changes each); 70% ETOH
(2 X 30 minute changes) This is a good stopping point: then I leave them in
a 3rd change of 70% overnight in the cold.
9. 2 X 15 minutes - 95% ETOH
10. 4 X 15 minutes - 100% ETOH
11. 2 X 15 minutes - propylene oxide
12. 2:1 propylene oxide:epon (overnight or minimum 2 hours)
13. 1:2 propylene oxide:epon (overnight or minimum 2-3 hours)
14. 100% epon - all day and then embed at end of day.
15. Polymerize in 60 degree oven overnight (18 hours). (I have actually
left them for 2 days in the oven and the blocks have been fine. I hesitate
to leave them too long; the blocks then get brittle)

I order my embedding components from Tousimis (Rockville, MD):
EPON 812 - Cat# 3131
DDSA - Cat# 3123
NMA - Cat# 3143
DMP-30 - Cat# 3103A

I store components under hood at rm. temperature and freeze aliquots of
complete epon. I use aliquots for the interim steps (i.e.with propylene
oxide) but make up fresh epon for embedding. I make up the epon on a stir
plate, stirring after addition of each component. I then put it under vacuum
to get rid of excess air bubbles.

I realize everyone's protocol is slightly different (judging from the
responses you've received), but as long as you maintain the structure of the
retina, you should have no problem with vacuoles or tears in the tissue.

Hope this helps.
Now...if you could send me a protocol for microwave processing, I would
greatly appreciate it!


Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

} -----Original Message-----
} From: Tindall, Randy D. [SMTP:TindallR-at-missouri.edu]
} Sent: Wednesday, August 21, 2002 10:14 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM/LM: Problems with cat retinal tissue
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} We are having some difficulty with getting good thick sections from feline
} retinal tissue. The sections look somewhat uneven in thickness (wavy), and
} in the nerve fiber layer and at the level of the inner limiting membrane
} there is vacuolization. There are also tears in some areas. Our standard
} processing procedures use 2% gluteraldehyde/2% paraformaldehyde in 0.1M
} cacodylate buffer, 1% osmium post-fix, 1% uranyl acetate en bloc stain,
} ethanol dehydration, and Spurr's/EPON resin. We are currently trying 2.5%
} gluteraldehyde in cacodylate (at 0.1 and 0.17M), as well as combining
} microwave fixation with our standard procedures.
}
} Our current procedures---microwave and otherwise---work very well with
} mouse retinal tissue, but cats are being contrary. If anyone has any
} tried and true techniques, I'd be very grateful to hear about them. It
} could save us tons of time and frustration as we develop a new set of
} protocols.
}
} Thanks very much.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}


From daemon Fri Aug 23 12:26:58 2002



From: R. Ann Bliss :      bliss5-at-llnl.gov
Date: Fri, 23 Aug 2002 10:19:22 -0700
Subject: clip art

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:

Any one have a line drawing of an electron microscope of one sort or
another? Maybe you know where I might get one?

TIA

Annie
--

+++++++++++++++++++++++++++++

R. Ann Bliss
Technician, Chemistry and Materials Science
Materials Science and Technology Division
Lawrence Livermore National Laboratory

_____________________________


From daemon Fri Aug 23 14:34:59 2002



From: Bruce Brinson :      brinson-at-rice.edu
Date: Fri, 23 Aug 2002 14:31:19 -0700
Subject: TEM,3mm disk

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is there someone who has or would be willing to provide me with 3mm
diameter disk cut from optical microscope slip covers. 30-50 pieces
would be nice. No problem paying a reasonable price for this service.
Please contact me off line if you can help us out.

thanks,
Bruce Brinson
Rice U.



From daemon Fri Aug 23 22:42:12 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Sat, 24 Aug 2002 16:37:50 -0400
Subject: Tetenal camera varnish 100912

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bruce, contact www.ssoptical.thomasregister.com or (260)749-9614. Be
specific in your requirements, as they are not a microscopy supplier.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Bruce Brinson {brinson-at-rice.edu}
To: MSA Listserver {microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 23, 2002 5:31 PM




-----Original Message-----
} From: Kuusisto, Ari [mailto:Ari.Kuusisto-at-PerkinElmer.com]
Sent: Friday, August 23, 2002 9:36 AM
To: MSA listserver

You might try the following,

http://www.norlandprod.com/adhesives/adhindex.html

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center, Room SS024
West Chester University of Pennsylvania
South Church Street & Rosedale Ave.
West Chester, PA, 19383
Phone & FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/

Hi,

I am sending this for a colleague of mine. He would be grateful if somebody
can help him.

'Tetenal has been discontinued their camera varnish 100912. Does anyone know
something similar varnish for gluing optical components?

__________________________________________________________
Mr Raimo Harju, M.Sc.,
Technology Lead, Photonics
Instrument Development
PerkinElmer Life Sciences
Wallac Oy
P.O.Box 10, FIN-20101 Turku, Finland
Phone: +358 2 2678111, Direct: +358 2 2678224, Fax: +358 2 2678530
Mobile: +358 40 7171629
E-mail: raimo.harju-at-perkinelmer.com,
Website:www.perkinelmer.com'


Ari Kuusisto, Research Physicist

PerkinElmer Life Sciences,
Wallac Oy

Phone: +358 2 267 8508 (direct)
+358 2 267 8111 (switchboard)
Fax: +358 2 267 8357
e-mail: Ari.Kuusisto-at-PerkinElmer.com
Ari.Kuusisto-at-Wallac.fi
Mail address: PO Box 10
FIN-20101 Turku,
Finland.

Street address: Laituri 4
Mustionkatu 6,
FIN-20750 TURKU
Finland





From daemon Mon Aug 26 02:49:03 2002



From: Renaat Dasseville :      renaat.dasseville-at-rug.ac.be
Date: Mon, 26 Aug 2002 09:29:23 +0200
Subject: SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am new to Scanning Electron Microscopy. We recently acquired a JEOL
5600LV. The specimens we work on are mainly diatoms and although we got a
demonstration on how to operate, it is mainly up to me to try to find the
ideal settings to obtain good imaging! So far I haven't been able to get
sharp focused images at high magnification (} x5000). Is there anyone out
there who is also working on diatoms and is willing to share experiences?

Thanks,

Renaat Dasseville
Protistology & Aquatic Ecology
Dept. Biology, University Gent
Krijgslaan 281, S8
9000 Gent, B E L G I U M
tel: +32 9 264 85 04
fax +32 9 264 85 99




From daemon Mon Aug 26 07:52:47 2002



From: msteglic-at-mail.mdanderson.org
Date: Mon, 26 Aug 2002 07:44:34 -0500
Subject: Re: clip art

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




If you can find an older version of Practical Electron Microscopy for
Biologists, second ed. by Meek, 1976, check out pages 108 and 109, they may be
of help.

Mannie Steglich
Tech Dir E M Lab
U T M D Anderson Cancer Center




From daemon Mon Aug 26 08:13:44 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 26 Aug 2002 06:06:35 -0700 (PDT)
Subject: EDS: Automatic LN2 Refilling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are in the market for automated filling equipment
for the liquid nitrogen dewar of our EDS unit. Iím
aware of the convenience having this device.... the
fact that you do not have to remember to fill the
unit,
and vacation filling. I wonder what experience others
may have on the downside of having this device, as
well as a source for buying such a unit.

Stu Smalinskas
Metallurgist
SKF NATC
46815 Port Street
Plymouth, Michigan 48170
(734) 414-6862

__________________________________________________
Do You Yahoo!?
Yahoo! Finance - Get real-time stock quotes
http://finance.yahoo.com


From daemon Mon Aug 26 08:27:52 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Mon, 26 Aug 2002 09:19:50 -0400
Subject: New SEM opproximate price

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning,
One of mine coworkers would like to find out the opprox. price for new SEM
with same features as mine.
EDS/WDS, backscattered detector, dot mapping, line profile.

Pavel



From daemon Mon Aug 26 09:50:00 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Mon, 26 Aug 2002 10:50:56 -0400
Subject: Re: SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Renaat;

Diatoms with cellular matter in place or skeletons?

John Twilley

Renaat Dasseville wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
}
} I am new to Scanning Electron Microscopy. We recently acquired a JEOL
} 5600LV. The specimens we work on are mainly diatoms and although we got a
} demonstration on how to operate, it is mainly up to me to try to find the
} ideal settings to obtain good imaging! So far I haven't been able to get
} sharp focused images at high magnification (} x5000). Is there anyone out
} there who is also working on diatoms and is willing to share experiences?
}
} Thanks,
}
} Renaat Dasseville
} Protistology & Aquatic Ecology
} Dept. Biology, University Gent
} Krijgslaan 281, S8
} 9000 Gent, B E L G I U M
} tel: +32 9 264 85 04
} fax +32 9 264 85 99





From daemon Mon Aug 26 10:35:19 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Mon, 26 Aug 2002 11:37:47 -0400
Subject: Re: EDS: Automatic LN2 Refilling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kestutis:

Because they activate automatically when unattended, they must allow for the
rapid release of boil-off and displacement of gas that comes with
refilling. This means that there is usually more of a problem with
condensation and ice buildup near the vent, which necessarily does not fit
as closely as a manual filler cap. Ice that falls into the cryostat and is
constantly moved by nitrogen bubbles can become a cause of noise in the
detector, increasing deadtime among other things. So you may exchange the
need for frequent filling for the need to clean out the cryostat
periodically.

There is also some chance that the valve will fail in the open position,
leading to overflow. One consideration in that case is the possibility that
someone responding to the malfunction in a confined or poorly ventilated
area could be overcome by the lack of oxygen before they realized the
danger.

John Twilley

Kestutis Smalinskas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are in the market for automated filling equipment
} for the liquid nitrogen dewar of our EDS unit. Iím
} aware of the convenience having this device.... the
} fact that you do not have to remember to fill the
} unit,
} and vacation filling. I wonder what experience others
} may have on the downside of having this device, as
} well as a source for buying such a unit.
}
} Stu Smalinskas
} Metallurgist
} SKF NATC
} 46815 Port Street
} Plymouth, Michigan 48170
} (734) 414-6862
}
} __________________________________________________
} Do You Yahoo!?
} Yahoo! Finance - Get real-time stock quotes
} http://finance.yahoo.com





From daemon Mon Aug 26 12:52:57 2002



From: JHoffpa464-at-aol.com
Date: Mon, 26 Aug 2002 13:44:04 -0400
Subject: Re: New SEM opproximate price

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


have you thought about contacting the company you bought yours from?


From daemon Mon Aug 26 13:02:13 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 26 Aug 2002 10:56:00 -0700 (PDT)
Subject: SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Renaat:

I use a JEOL 5800LV microscope. Though most of my
subjects are conductive, I do - at times - use the LV
feature for non-conductive samples. I assume diatoms
are non-conductive and your question relates to using
the LV mode.

A magnification of 5000X is a lot to ask from this
instrument. To maximize resolution, important
parameters to set are:

- High spot size. I set mine to maximum.
- Use the smaller (150 micron) orifice for
differential pressure.
- Low working distance.
- High accelerating voltage.
- Low chamber pressure.

All these parameters need to be balanced against
sample integrity, sample charging, and gain when
acquiring an image.


Stu Smalinskas
Metallurgist
SKF NATC
46815 Port Street
Plymouth, Michigan 48170
ph.(734) 414-6862

__________________________________________________
Do You Yahoo!?
Yahoo! Finance - Get real-time stock quotes
http://finance.yahoo.com


From daemon Mon Aug 26 14:04:50 2002



From: Leslie Cummins :      gunther-at-aecom.yu.edu
Date: Mon, 26 Aug 2002 15:00:55 -0400
Subject: TEM - macrophage collection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers

I am having a problem attempting to scrape and collect primary macrophage
grown on a 60mm plastic petri dish.

The cells are fixed in 4% paraformaldehyde, blocked with 0.05M glycine,
buffer rinsed, then water rinsed. I am using a disposable cell scraper
from Fisher and scraping them in a very small amount of water, almost
dry. When I collect the water with a pipet and transfer to an eppendof
tube to spin the pellet down, there seem to be no cells. I have tried
spinning them very hard, or very long but to no avail. I use this protocol
on other cell types and get wonderful pellets, with plenty of sample to
work with.

It seems to be cell type specific and I was wondering if anyone had any
ideas to help me get a usable, visible pellet. I am trying to do ultrathin
cryosections, so I am slightly limited in my options.

Thanks in advance.
Leslie



Leslie Gunther Cummins
Analytical Imaging Facility
Albert Einstein College of Medicine
1300 Morris Park Ave.
Bronx, NY 10461
718-430-3547

http://www.aecom.yu.edu/aif/



From daemon Mon Aug 26 14:39:10 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 26 Aug 2002 15:37:45 -0400
Subject: New SEM opproximate price

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pavel;

Let's start by what your samples are like, what magnification and resolution
you need, unless money is no object. What system are you flying at the
moment?

Peter

-----Original Message-----
} From: ATC SEM Laboratory [mailto:atcsem-at-earthlink.net]
Sent: Monday, August 26, 2002 9:20 AM
To: Microscopy-at-sparc5.microscopy.com


Good morning,
One of mine coworkers would like to find out the opprox. price for new SEM
with same features as mine.
EDS/WDS, backscattered detector, dot mapping, line profile.

Pavel



From daemon Mon Aug 26 14:47:11 2002



From: Benyam Estifanos :      benyam-at-mac.com
Date: Mon, 26 Aug 2002 16:26:55 -0400
Subject: TEM: Indexing of Electron Diffraction Patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Years ago, I shot some images of diatoms, but I coated them with Au/Pd. Is
there a good reason for not coating a diatom intended for SEM observation?

Ron
----- Original Message -----
} From: "Kestutis Smalinskas" {smalinskas-at-yahoo.com}
To: {Microscopy-at-sparc5.microscopy.com} ; {renaat.dasseville-at-rug.ac.be}
Sent: Monday, August 26, 2002 1:56 PM


Dear Colleagues,

I have developed a computer program to index electron diffraction patterns.
IndED is a powerful tool for indexing the electron diffraction
patters. For given lattice parameters, it calculates the most
possible set of all symmetrically equivalent zones.

The demo programs can be downloaded from M & M software library or
from the following home page:
http://homepage.mac.com/benyam/

The programs run only on Macs (MacOS).

Benyam


From daemon Mon Aug 26 16:27:35 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 27 Aug 2002 09:19:30 +1200
Subject: EPMA Job

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

There is an EPMA job going at the University of Otago, Dunedin, New Zealand, for a
geologist/microprobist. JXA-8600.

It's a Scientific Officer (ie non-academic) job, in the Department of Geology, ref
GG02/581.

The website doesn't seem to work properly (at least for me) www.otago.ac.nz/jobs,
but one can email the Human Resources Division
katherine.van-der-vlier-at-stonebow.otago.ac.nz or the Head of Department, Prof Alan
Cooper, alan.cooper-at-stonebow.otago.nz.

I thought I'd post this as over the years people have sometimes expressed an
interest in working in NZ.

There are three EMPAs in the country - a 733 in Wellington, an 840a in Auckland,
and the Otago 8600.

Applications close this Friday August 30, and don't forget that NZ is 12 hours ahead
of GMT.

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Aug 26 18:33:14 2002



From: Hiromi Konishi :      hkonishi-at-asu.edu (by way of MicroscopyListserver)
Date: Mon, 26 Aug 2002 18:21:27 -0500
Subject: Terminology of Crystallographic Axis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am writing about the terminology of crystallographic axis.
The International Tables of Crystallography refers to the X-, Y-,
Z-axes of a crystal with basis vectors a, b, and c along these axes,
but not a-, b-, and c-axes. However, many authors use a-, b-, and
c-axes in TEM papers instead of X, Y, and Z axes. I would like to
know why electron crystallographers prefer to use the terminology of
a, b, and c-axes. Is there any reasons related to crystallography or
history of TEM? Who defined a, b, and c-axes?

I would appreciate any comments on the terminology.

Hiromi Konishi
Arizona State University


From daemon Mon Aug 26 18:44:10 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 26 Aug 2002 16:39:03 -0700
Subject: Re: EDS: Automatic LN2 Refilling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Take a look at the VBS Safe Fill system. It can save as
much as 50% LN2 over manual filling. And it is safe.

http://www.vbsflex.com/ADF10.pdf

gary g.

No financial interest.


At 06:06 AM 8/26/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Aug 26 18:52:33 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 26 Aug 2002 18:46:16 -0500
Subject: SEM: digital upgrade to analog SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking to replace (upgrade) our digital image capture system
on our Hitachi S570 SEM and would be interested in hearing from users
of various systems.

What system do you have? Are you happy with it (performance and
service-wise)? How much did it cost?

Many thanks for sharing your experience.

PS: commercial vendors are also invited to contact us as well.

John B.

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Aug 27 00:49:23 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Tue, 27 Aug 2002 07:37:02 +0200
Subject: Re: SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ranaat

I used a JSM 840 SEM in the past for Diatom work and c as well as
gold-palladium coating. We got beautiful results. In LV mode we did find
with the ESEM fei that working distance and the correct vacuum is very
important. Due to the beam scattering more and the detectors not being that
efficient you will have to play a bit. Reduce working distance and
surprisingly increase spot size. KV appeasers to prefer 12 above 10. In
ESEM mode we got clear images at 20 000 times, even new students did not
battle.
I love the ESEM when it comes to biological samples.


Mr. S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana

Phone : +267 355 2426
Mobile : +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw



-----Original Message-----
} From: John Twilley [mailto:jtwilley-at-sprynet.com]
Sent: Monday, August 26, 2002 4:51 PM
To: Renaat Dasseville
Cc: Microscopy Listserver


Renaat;

Diatoms with cellular matter in place or skeletons?

John Twilley

Renaat Dasseville wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
}
} I am new to Scanning Electron Microscopy. We recently acquired a JEOL
} 5600LV. The specimens we work on are mainly diatoms and although we got a
} demonstration on how to operate, it is mainly up to me to try to find the
} ideal settings to obtain good imaging! So far I haven't been able to get
} sharp focused images at high magnification (} x5000). Is there anyone out
} there who is also working on diatoms and is willing to share experiences?
}
} Thanks,
}
} Renaat Dasseville
} Protistology & Aquatic Ecology
} Dept. Biology, University Gent
} Krijgslaan 281, S8
} 9000 Gent, B E L G I U M
} tel: +32 9 264 85 04
} fax +32 9 264 85 99





From daemon Tue Aug 27 06:25:16 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Tue, 27 Aug 2002 07:07:54 -0400
Subject: Re: New SEM opproximate price

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I appreciate the information. I think, I've got more than enough.
Thank you all very much for comprehensive answers.

Pavel



From daemon Tue Aug 27 07:20:57 2002



From: Rich Fiore :      rafiore-at-unity.ncsu.edu (by way of
Date: Tue, 27 Aug 2002 07:09:43 -0500
Subject: Video frame grabber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a video frame grabber card for a PC (PCI slot) that will do
integration and work with NIH Image. Thanks in advance for your help.

Rich Fiore, EM & SPM
NC State University
Analytical Instrumentation Facility
2410 Campus Shore Drive
318 EGRC, Campus Box 7531
Raleigh, NC 27695
Tel: 919-515-2348
Fax: 919-515-6965
Email: rich_fiore-at-ncsu.edu


From daemon Tue Aug 27 08:03:54 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Tue, 27 Aug 2002 05:56:20 -0700 (PDT)
Subject: SEM: digital upgrade to analog SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John:

We use a thermal printer (Sony Video Graphic Printer,
Model UP-890MD) on our SEM. Last I heard, they have a
Model 895 that cost $895, much less than the $6000 we
paid four years ago.

I know it’s not a digital capture system you’re asking
about, but you may want to consider it. After the
capital cost, prints are roughly 20 cents apiece,
which can then be scanned.

I really like this system, since prints are nearly
instantaneous with good resolution. The biggest
convenience is that during my semming sessions I like
to “fire away” with pictures, then pick and choose the
images I’ll present to my client.

Stu Smalinskas
Metallurgist
SKF NATC
Plymouth, Michigan
stu.smalinskas-at-skf.com


__________________________________________________
Do You Yahoo!?
Yahoo! Finance - Get real-time stock quotes
http://finance.yahoo.com


From daemon Tue Aug 27 08:05:29 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Tue, 27 Aug 2002 07:59:03 -0500
Subject: SEM roughing pump problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a TOPCON SM-510 low vacuum SEM, it has a diffusion pump with an
Edward's roughing pump. I have been having a problem with the info on the
digital captured photo disappearing. The only way I can get the info to
reappear is to shut the SEM down for about one hour then restarting it, I
have called the repair people about that problem. Last weekend I shut the
SEM down and when I tried to start it up Monday, no joy. the vacuum
wouldn't go low enough to start. After checking the o-rings and vacuum
hoses I noticed there were waves of oil in the vacuum hoses. After draining
them and running some ethanol through the lines and changing the roughing
pump vacuum pump oil I managed to get the SEM up and running.
My question is about the roughing pump it has two plastic screw in
lids, one to fill up the pump and the other one labeled ballast. What is
the ballast lid for? Its spring loaded and can be left open or closed. I
have been running the pump with it closed since that the way the SEM was
set up, the company setup person didn't know what it was for either. Would
this have anything to do with the oil back up? I suspect an o-ring slipped
in the SEM valving system but I have always wondered what the ballast lid
is for? Does anyone know?
Terry Ellis
Hallmark Cards Inc.
tellis2-at-hallmark cards
816-545-6573



From daemon Tue Aug 27 08:35:58 2002



From: Emmanuelle Roux :      eroux-at-caprion.com
Date: Tue, 27 Aug 2002 09:27:09 -0400
Subject: Macrophages

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Leslie,
It will look like a silly question but have you looked at your "invisible pellet" under a microscope or just by looking at your tube and not seeing it? I remember working with cultured macrophages and while doing an immunolabeling "loosing" my sample (I could not see a pellet anymore at some point of the experiment). But after putting the "invisible pellet" on a slide (cytospots) and looking under a microscope the cells were there! For some reason they became invisible for the eyes but were still there.
Emmanuelle

Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620



From daemon Tue Aug 27 08:58:51 2002



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 27 Aug 2002 09:51:40 -0400
Subject: RE: TEM - macrophage collection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you thought about growing them on a coverslip and then processsing the
cells intact--the final stage would involve inverting a beem capsule with
epon on top of it, polymerizing it, and then using liquid nitrogen to pop
the capsul off. Cells adhere nicely to the epon and then you can section.

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

} -----Original Message-----
} From: Leslie Cummins [SMTP:gunther-at-aecom.yu.edu]
} Sent: Monday, August 26, 2002 3:01 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM - macrophage collection
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Listers
}
} I am having a problem attempting to scrape and collect primary macrophage
} grown on a 60mm plastic petri dish.
}
} The cells are fixed in 4% paraformaldehyde, blocked with 0.05M glycine,
} buffer rinsed, then water rinsed. I am using a disposable cell scraper
} from Fisher and scraping them in a very small amount of water, almost
} dry. When I collect the water with a pipet and transfer to an eppendof
} tube to spin the pellet down, there seem to be no cells. I have tried
} spinning them very hard, or very long but to no avail. I use this
} protocol
} on other cell types and get wonderful pellets, with plenty of sample to
} work with.
}
} It seems to be cell type specific and I was wondering if anyone had any
} ideas to help me get a usable, visible pellet. I am trying to do
} ultrathin
} cryosections, so I am slightly limited in my options.
}
} Thanks in advance.
} Leslie
}
}
}
} Leslie Gunther Cummins
} Analytical Imaging Facility
} Albert Einstein College of Medicine
} 1300 Morris Park Ave.
} Bronx, NY 10461
} 718-430-3547
}
} http://www.aecom.yu.edu/aif/
}


From daemon Tue Aug 27 10:50:30 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 27 Aug 2002 10:41:13 -0500
Subject: Re: SEM: digital upgrade to analog SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree that your thermal printer is convenient solution to a problem. It
is nice to get prints quickly and that cheaply. I also found the 895 you
listed. I didn't check a lot of sites. The one I did check listed the unit
for under $1400 which still beats your $6000 cost of 4 years ago.

First, I should say that not all old SEMs can support a video printer. Our
JEOL-840A as first delivered did not have a video signal that could be
digitized. We added an option that provided us a video signal, but it was
pricey.

You also need to be available of the limitations of video printers. The
printers may be capable of many pixels of resolution, but the limiting
factor is the resolution and quality of the video signal. NTSC video only
specifies about 500 lines of resolution. (Your printer specifies a maximum
of 608 lines that it can render.) That is acceptable for many purposes, but
most users will want more for their digitized SEMs.

There is also a quality issue. The signal-to-noise ratio for an SEM can be
quite low when setup for high resolution imaging. Therefor, it is necessary
to extend the acquisition time to integrate or average the signal over
time. I don't know how that is done with your system. Our Hitachi has a
digital frame store that can retain the entire image and display it at
video speeds. But by the time we do that, we might as well use our regular
digital means to record the image (Quartz PCI or Oxford Instruments
AutoBeam). I know those systems can digitize with 8 bits of precision. I
don't know if the video signal can afford that much precision. (I
understand the Sony printer is spec'ed for 256 gray levels, 8-bits,
assuming that such a good signal is provided to it.

Scanning presents another problem. It means one more link in the chain on
the way to digital. Now there is the question of S/N in the SEM signal and
its integration on the SEM side, the transmission of that signal to the
Sony printer, the digitization of that signal by the printer, the faithful
rendition of that level on the printer paper, and the digitization of that
printed signal by a scanner. That is a LOT of steps. A normal digitization
approach catches the signal early on in the SEM avoiding the other steps
which can introduce errors.

Having said all that, the video printer sounds quite convenient and may
work for some users, but it should probably not be classed as a
digitization system.

Warren

At 05:56 AM 8/27/02 -0700, you wrote:

} John:
}
} We use a thermal printer (Sony Video Graphic Printer,
} Model UP-890MD) on our SEM. Last I heard, they have a
} Model 895 that cost $895, much less than the $6000 we
} paid four years ago.
}
} I know it's not a digital capture system you're asking
} about, but you may want to consider it. After the
} capital cost, prints are roughly 20 cents apiece,
} which can then be scanned.
}
} I really like this system, since prints are nearly
} instantaneous with good resolution. The biggest
} convenience is that during my semming sessions I like
} to "fire away" with pictures, then pick and choose the
} images I'll present to my client.
}
} Stu Smalinskas
} Metallurgist
} SKF NATC
} Plymouth, Michigan
} stu.smalinskas-at-skf.com




From daemon Tue Aug 27 11:33:10 2002



From: simkin-at-egr.msu.edu
Date: Tue, 27 Aug 2002 12:25:19 -0400 (EDT)
Subject: Re: SEM roughing pump problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The ballast, as I recall, is essentially a leak valve that leaks into the first
stage of the pump so that there is a constant flow of gas through the pump. The
idea is that this flow keeps down the concentration of condensible gasses/fluids
into the pump oil. Opening the ballast will increase the ultimate pressure of
the vacuum attainable by your roughing pump, which is why it us usually kept
closed unless needed.
From your description, however, it sounds like the valve that has failed
on your pump is the anti-suckback valve. That is supposed to seal the pump off
from your roughing line vacuum when the pump shuts down; if this doesn't work,
then the pressure difference between the air outside and the line vacuum
inside ends up blowing most of the oil out of your pump and into your roughing
line, just as you've seen. If you can stand keeping your roughing line under
vacuum, then a decent short-term approach is to simply hardwire your roughing
pump so it always runs; this keeps vacuum on both sides of the valve, so no
suckback. The long term solution, however, is to fix the valve or replace the
pump.

Cheers,
Ben Simkin (simkin-at-egr.msu.edu)
Michigan State University dpt. Chem. Eng. and Materials Science


From daemon Tue Aug 27 12:07:45 2002



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Tue, 27 Aug 2002 11:59:20 -0500
Subject: Thanks - Adding a digital camera to an optical microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Once again I am most impressed with this list. I have received a pile of
good information on this topic and now merely {g} have to sift through the
many options I have been presented and make a good choice. Gladly this list
has provided me with the many good options as well as information to use in
making the final decision.

Thanks!

Richard Shalvoy
Arch Chemicals, Inc.
350 Knotter Drive
Cheshire, CT 06410
(203) 271-4394
rbshalvoy-at-archchemicals.com



From daemon Tue Aug 27 13:55:58 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Tue, 27 Aug 2002 13:46:44 -0500
Subject: Styrene-methacrylate/SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listies,

I'm attempting to view cross sections of feather barbs on the SEM. We need
to analyze nano vacuole diameters. However, cutting the barbs with a blade
smears the keratin at the surface, thus covering the vacuoles that we need
to collect data from.

I havn't tried crude freeze fracture because I need a flat surface.

One procedure recomends infiltrating with styrene-methacrylate, sectioning
a flat surface with a diamond knife and then removing the resin with toluene
and acetone.

Has anyone out there worked with this resin?

Where can I find it?

Help!
Tim Quinn
University of Kansas
Program Assistant/Microscopists/Histo-Lab Lab Manager
Ornithology Dept.
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556/785-331-4107
tquinn-at-ku.edu


From daemon Tue Aug 27 13:59:46 2002



From: Rich Fiore :      rafiore-at-unity.ncsu.edu (by way of\
Date: Tue, 27 Aug 2002 07:09:43 -0500
Subject: Video frame grabber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am looking for a video frame grabber card for a PC (PCI slot) that will do
integration and work with NIH Image. Thanks in advance for your help.

Rich Fiore, EM & SPM
NC State University
Analytical Instrumentation Facility
2410 Campus Shore Drive
318 EGRC, Campus Box 7531
Raleigh, NC 27695
Tel: 919-515-2348
Fax: 919-515-6965
Email: rich_fiore-at-ncsu.edu



From daemon Tue Aug 27 16:16:54 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 27 Aug 2002 14:07:47 -0700
Subject: Re: SEM roughing pump problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Terry,
The ballast valve on a rotary pump lets a small amount of air into the pump,
the same as if you had a small leak in your system. It is used to clean
solvents or water vapour out of the oil in the pump by bubbling air throught
the oil for about 10 to 15 minutes. It is only used on brand new oil (when
you change the oil in the pump) or when you suspect the oil has been exposed
to a solvent. Leave it open for no more than 15 minutes. It will degrade the
ultimate vacuum slightly, while it is open. For normal operation, leave it
closed.
I cannot see how this would have anything to do with your digital image
information. That is probably a software switch somewhere that "burns" the
info into the digital photo and that has not been set.
Oil in the foreline means you are getting bad backstreaming into the system.
I would recommend a foreline trap to prevent that. It can cause a lot of
problems in the vacuum system. I also never turn the microsope off, except
for maintenance. I just turn off the displays and monitors.
At 07:59 AM 08/27/2002 -0500, you wrote:
} We have a TOPCON SM-510 low vacuum SEM, it has a diffusion pump with an
} Edward's roughing pump. I have been having a problem with the info on the
} digital captured photo disappearing. The only way I can get the info to
} reappear is to shut the SEM down for about one hour then restarting it, I
} have called the repair people about that problem. Last weekend I shut the
} SEM down and when I tried to start it up Monday, no joy. the vacuum
} wouldn't go low enough to start. After checking the o-rings and vacuum
} hoses I noticed there were waves of oil in the vacuum hoses. After draining
} them and running some ethanol through the lines and changing the roughing
} pump vacuum pump oil I managed to get the SEM up and running.
} My question is about the roughing pump it has two plastic screw in
} lids, one to fill up the pump and the other one labeled ballast. What is
} the ballast lid for? Its spring loaded and can be left open or closed. I
} have been running the pump with it closed since that the way the SEM was
} set up, the company setup person didn't know what it was for either. Would
} this have anything to do with the oil back up? I suspect an o-ring slipped
} in the SEM valving system but I have always wondered what the ballast lid
} is for? Does anyone know?
} Terry Ellis
} Hallmark Cards Inc.
} tellis2-at-hallmark cards
} 816-545-6573
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Aug 27 17:02:12 2002



From: mmcconne-at-engineering.uiowa.edu (by way of MicroscopyListserver)
Date: Tue, 27 Aug 2002 16:51:34 -0500
Subject: Ask-A-Microscopist: suggestions on types of microscopy to use

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mmcconne-at-engineering.uiowa.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
August 27, 2002 at 12:46:49
---------------------------------------------------------------------------

Email: mmcconne-at-engineering.uiowa.edu
Name: Michael McConney

Organization: University of Iowa

Education: Undergraduate College

Location: Iowa City, IA

Question: I was wondering if you had suggestions on types of
microscopy to use. The project I am working on involves bonding
collagen IV to a linker chemical which is bonded to a polymer
surface. The collagen bonding is all over the surface of the
polymer. I am interested in obtaining the conformation of the
collagen on the surface of the polymer, not the bending of the
protein, but the geometry of the arranged collagen on the surface.
We have used atomic-force techniques with mixed succes. I was
wondering if you knew of any alternatives we could use?

Thank you very much for your time

-Michael McConney

---------------------------------------------------------------------------


From daemon Tue Aug 27 18:13:22 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 27 Aug 2002 16:07:38 -0700
Subject: Re: Styrene-methacrylate/SEM

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You might try thin CA (cyanoacrylate).....crazy glue,
super glue, etc. But this is the thin style. Hobby shops
have it.

gary g.


At 11:46 AM 8/27/2002, you wrote:

} Dear Listies,
}
} I'm attempting to view cross sections of feather barbs on the SEM. We need
} to analyze nano vacuole diameters. However, cutting the barbs with a blade
} smears the keratin at the surface, thus covering the vacuoles that we need
} to collect data from.
}
} I havn't tried crude freeze fracture because I need a flat surface.
}
} One procedure recomends infiltrating with styrene-methacrylate, sectioning
} a flat surface with a diamond knife and then removing the resin with toluene
} and acetone.
}
} Has anyone out there worked with this resin?
}
} Where can I find it?
}
} Help!
} Tim Quinn
} University of Kansas
} Program Assistant/Microscopists/Histo-Lab Lab Manager
} Ornithology Dept.
} Natural History Museum and Biodiversity Research Center
} Dyche Hall Room 414
} Lawrence, KS 6604-2454
} 785-864-4556/785-331-4107
} tquinn-at-ku.edu



From daemon Tue Aug 27 18:26:38 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 27 Aug 2002 16:21:59 -0700
Subject: Re: Video frame grabber

Contents Retrieved from Microscopy Listserver Archives
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Why not use Scion Image (free updated version of NIH Image)
and purchase a Scion frame grabber board?

gary g.


At 05:09 AM 8/27/2002, you wrote:

} I am looking for a video frame grabber card for a PC (PCI slot) that will do
} integration and work with NIH Image. Thanks in advance for your help.
}
} Rich Fiore, EM & SPM
} NC State University
} Analytical Instrumentation Facility
} 2410 Campus Shore Drive
} 318 EGRC, Campus Box 7531
} Raleigh, NC 27695
} Tel: 919-515-2348
} Fax: 919-515-6965
} Email: rich_fiore-at-ncsu.edu
}



From daemon Tue Aug 27 23:22:47 2002



From: RCHIOVETTI-at-aol.com
Date: Wed, 28 Aug 2002 00:14:20 EDT
Subject: Re: Video frame grabber

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 08/27/2002 5:26:54 AM US Mountain Standard Time,
rafiore-at-unity.ncsu.edu writes:

{ { I am looking for a video frame grabber card for a PC (PCI slot) that will
do
integration and work with NIH Image. Thanks in advance for your help.

Rich Fiore, EM & SPM
NC State University
Analytical Instrumentation Facility
2410 Campus Shore Drive
318 EGRC, Campus Box 7531
Raleigh, NC 27695
Tel: 919-515-2348
Fax: 919-515-6965
Email: rich_fiore-at-ncsu.edu
} }


Rich,

The only version of NIH Image that I know of is "Scion Image." To work
within Scion Image, you need a Scion framegrabber, probably something like
their CG-7 board, which sells for about $1700.

Of course, you could get another framegrabber board that would allow you to
capture images, then with Scion Image running you could open the captured pic
to work with it. I think you need either a .bmp or .tif file format to work
in Image in this fashion.

We've had good luck with Integral framegrabber boards. They make two
versions, the only difference is the types of video input accepted. Their
FlashBus MV Pro (about $895) accepts RGB, Composite Video and S-Video (a.k.a.
Y/C video). The FlashBus MV Pro Lite (about $600) accepts Composite Video
and S-Video. Other framegrabbers are also available from Integral that have
multiple inputs, if you need to run more than one device (scanner + camera,
etc.)

Contact Info:

Scion: 301-695-7870 {www.scioncorp.com}
Integral: 317-845-9242 {www.integraltech.com}

Hope this helps!

Cheers,

Bob Chiovetti


From daemon Tue Aug 27 23:54:43 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 27 Aug 2002 21:49:47 -0700
Subject: Re: New SEM opproximate price

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Between used and new, the cost could range between $1 to
$500K,. not including shipping. Your post is rather ambiguous
relative to exactly what you are looking for. Is this a SEM or FESEM?
Big difference. Turbo or diff pump?, etc., etc., blah, blah.


gg



At 06:19 AM 8/26/2002, you wrote:
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From daemon Wed Aug 28 10:04:34 2002



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Wed, 28 Aug 2002 16:56:57 +0200
Subject: Probe analytical problem

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Hallo Folks,
Here's a baffling thing for you to mull over. I'm after some
pointers after scratching my head for a couple of days. Here goes. We
have a JEOL 733 with 4 WD spectrometers. We've the simple task of
analysing some olivine crystals for their major (Si, Mg, Fe) and trace
(Ca, Ni, Mn) elements. This should be a very simple, rudimentary and
stressless thing to do, and normally is. The sad thing is, things aren't
going normally. Standardisation is as simple as possible with as few
standards as possible, and checked rigorously using materials for this
purpose mounted in our standard block. However, when we go to the actual
unknowns, Fe is sytematically about 3 wt % lower than expected for a
stoichiometric olivine assuming the Mg and Si are ok. We can make this
assumption because for an olivine of a specified SiO2 content we would
expect a specified MgO content, and this checks out. For the given FeO
content we would expect lower MgO and SiO2 contents, and this would
simply make the totals worse. I've checked out the hardware and the fact
that the calibration works for unknowns on the standard block suggests
to me that there is no problem with the analytical protocol we're using.
I considered C coating but thought that this is more likely to affect
attenuation of the lower energy X-rays such as Si and Mg.
Has anyone some suggestions on what the cause of this
strange behaviour for a very simple mineral could be?
Thanks,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (try your luck!)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA




From daemon Wed Aug 28 10:04:34 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Tue, 27 Aug 2002 07:07:54 -0400
Subject: Re: New SEM opproximate price

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I appreciate the information. I think, I've got more than enough.
Thank you all very much for comprehensive answers.

Pavel




From daemon Wed Aug 28 10:19:50 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Wed, 28 Aug 2002 10:13:05 -0500
Subject: Analysis Routine

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Group,

This question is a little off the norm but I thought someone may have done this before.

I am looking for a math routine that with take raw x-ray data in text format (counts vs. deg angle) and process it to give me the following:

1) replot of the curves
2) identify peak locations (angle)
3) do a background subtraction
4) find the area under the peak curves

I understand that this could possibly be done with Microsoft Excel which would be ideal. My calculus is a bit fuzzy so any help would be greatly appreciated.

Roy Beavers
Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, Tx. 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-mail.smu.edu



From daemon Wed Aug 28 10:54:00 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 28 Aug 2002 08:41:54 -0700
Subject: Re: Styrene-methacrylate/SEM

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I'd try LR White; it's a proprietary acrylic that adheres fairly well to
keratin. Try a glass knife before you buy a diamond.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Aug 28 10:57:21 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 28 Aug 2002 08:52:39 -0700
Subject: Re: Video frame grabber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Another option is to buy a Matrox Meteor-2 grabber ($545)
and then import the captured image to Scion or any other
compatible program.

gary



At 09:14 PM 8/27/2002, you wrote:
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Aug 28 12:06:41 2002



From: Todd Kostman :      kostman-at-vaxa.cis.uwosh.edu
Date: Wed, 28 Aug 2002 11:58:06 -0500
Subject: conversion of SN-2460 to digital

Contents Retrieved from Microscopy Listserver Archives
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Greetings all,

I am looking into getting digital output from a Hitachi SN-2460 SEM.
Has anyone out there converted this particular scope? Any suggestions?

Another idea a colleague and I tossed around was upgrading the EDS
system and using it as a source of digital image files. We currently
have a Noran Voyager system with a Sun Microsystems workstation. I am
sure that a Windows based system is now available-does anyone know if it
is possible, and at what price, to simply buy a new computer and
software package and connect it to the existing detector? Any
suggestions would be welcome.

Sincerely,


Todd

Todd A. Kostman, Ph.D.
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
University of Wisconsin Oshkosh
800 Algoma Blvd
Oshkosh, WI 54901-8640
ph; (920) 424-7301
fax: (920) 424-1101



From daemon Wed Aug 28 14:30:03 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Wed, 28 Aug 2002 15:16:43 -0400
Subject: What causes uneven brightness in epifluorescence microscope image

Contents Retrieved from Microscopy Listserver Archives
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This question is relevant to light microscopes in general, but I am posing
it in the context of epifluorescence microscopy using a fiber-optic light
guide and imaging using a square CCD array:

The image of a uniform field will be brightest near the center and fade away
at the edges and corners. Why is this?

I assumed this variation in brightness was due to uneven illumination caused
by a finite light source and the design of the illuminator optics. However,
a co-worker recently showed me an article that mentioned vignetting in
astronomical telescopes---uniformly bright objects appear brighter in the
center of the field because the cone of rays the telescope can accept from
an on-axis object is larger than that from an off-axis object. To what
extent might vignetting cause uneven illumination in light microscopes? To
what extent might vignetting result in brightness variation in the image of
a uniformly illuminated field?

Everett Ramer
Cellomics, Inc.
Pittsburgh, PA


From daemon Wed Aug 28 14:36:31 2002



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 28 Aug 2002 15:36:35 -0400
Subject: Fwd: Re: SEM roughing pump problem

Contents Retrieved from Microscopy Listserver Archives
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} Date: Tue, 27 Aug 2002 16:19:31 -0400
} To: "Terry E Ellis" {tellis2-at-hallmark.com}
} From: Wil Bigelow {bigelow-at-engin.umich.edu}
} Subject: Re: SEM roughing pump problem
} Cc:
} Bcc:
} X-Attachments:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Wed Aug 28 15:17:48 2002



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 28 Aug 2002 15:20:55 -0500
Subject: RMC ultramicrotome service

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Does Boeckeler Instruments have anyone local to St. Louis, MO that can do
preventative maintenance on RMC MT-X or MT-XL ultramicrotomes?

Does anyone know of any third-party technicians that will service them in
this area?

I'll contact Boeckeler, but I'd like to know if I have any other options.

Thanks,

Jaclynn M. Lett
Staff Technician, Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Harolds W. Siebens Hearing Research Center
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu

314-977-0257


From daemon Wed Aug 28 16:10:33 2002



From: Goheen, Michael P. :      mgoheen-at-iupui.edu
Date: Wed, 28 Aug 2002 16:02:40 -0500
Subject: Digital Imaging for TEM in Diagnostic Pathology Lab

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Dear Listservers,

We would like feedback on digital camera systems being used on TEM's in Diagnostic Pathology service labs. We have a growing Renal pathology service and want to utilize digital imaging to decrease turn around time and increase throughput. We have a lot of literature from vendors but need "real world" experiences. Please reply directly to:


Mike Goheen
Dept. of Pathology & Lab Medicine
Indiana University School of Medicine

mgoheen-at-iupui.edu

Thanks!


From daemon Wed Aug 28 17:07:38 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Wed, 28 Aug 2002 15:00:40 -0700
Subject: Occupational injury?

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This is primarily directed at people who do TEM, though it could well apply
to other histologists and confocal-users as well. For many years I was an EM
(and other kinds of imaging) technician, and a friend of mine still is. In
the last few years we have both developed something that seems like RSI. In
both cases, the disc between cervical vertebrae 5 and 6 is compressed,
causing inflamed tissue to impinge on the nerve. This causes a great deal of
pain in the area around the right scapula and in the right shoulder and arm,
with a focal point at the top of the forearm.
Do others on this list have the same problem? If it is common among EM
and microscopy people, and not in the rest of the population, it could be
the result of the stresses involved in sectioning, various kinds of
microscopy, use of computers for graphic work etc., for hours at a time. I
am asking mainly out of curiosity, but if it does turn out to be an
occupational hazard, then people could try to prevent it - it really is
quite unpleasant. Sorry about the cross-posting, but I think the overlap
among the groups is not 100%.

Lesley Weston.



From daemon Wed Aug 28 17:32:40 2002



From: Bob Roberts :      bobrobs-at-cox.net
Date: Wed, 28 Aug 2002 15:25:11 -0700
Subject: Conversion of SN2460 to digital

Contents Retrieved from Microscopy Listserver Archives
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Todd,

I know that Emispec Systems, Inc. has a PC based imaging/analyzer that has
been interfaced to this particular model instrument. (www.emispec.com). You
can directly plug your Noran detector into their digital pulse processor and
acquire digital images and x-ray maps (spectrum imaging) into a Windows
based computer.

Disclaimer: I have no financial interest in this company; just a satisfied
user.

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St
Topeka, Kansas 66617-1780
785.246.1232
www.emlabservices.com




From daemon Wed Aug 28 17:38:20 2002



From: James Talbot :      james-at-ktgeo.com
Date: Wed, 28 Aug 2002 17:33:55 -0500
Subject: Re: Analysis Routine

Contents Retrieved from Microscopy Listserver Archives
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Roy-

By X-ray data I assume you mean X-ray Diffraction data. There is a web
page that has a bunch of diffraction software
(http://www.ccp14.ac.uk/solution/peakprofiling/) There are links to
free and commercial software.

Sincerely,
James Talbot

K/T GeoServices, Inc.
Bulk and Clay Mineralogy by X-ray Diffraction
Argyle, Texas
(940) 597-9076
web site: http://www.ktgeo.com/



Beavers, Roy wrote:

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From daemon Wed Aug 28 18:19:22 2002



From: CHEN CHEN :      cchen5-at-jhmi.edu
Date: Wed, 28 Aug 2002 19:14:26 -0400
Subject: for cryoEM facility

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Hi, everyone,
Does anybody know if there is cryo-electron microscopy facility near the DC-Baltimore area? Such as in UMaryland, Upenn, Johns Hopkins, NIH, HHMI, Delaware, Virginia and etc.? We want to do some kind of this work, and looking for collaborators.

Thanks

Chen Chen



From daemon Wed Aug 28 20:56:06 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 28 Aug 2002 22:41:44 -0400
Subject: conversion of SN-2460 to digital

Contents Retrieved from Microscopy Listserver Archives
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Hi, Roy

Are we talking XRD or WDS/EPMA here?

cheers

rtch






Todd;

I have done as you mentioned on a Hitachi S570. That is, used the EDX
system's imaging and beam control as a capture vehicle. The only issue at
the time was the resolution of the captured image relative to acquisition
time since the processing capability was based on an old microprocessor and
motherboard in the EDX hardware. In the older SEMs such as the Hitachi
S570, there is no readily available port to grab the horizontal and vertical
scan information and hence the video board had to be physically spliced into
and the electronic levels made compatible with the EDX electronics. It was
a messy affair but ultimately did work. The system worked on a Win95
platform.

The cost to do this on your particular microscope will likely be a function
of who will be doing it, EDX vendor or graduate student. If you have an
electrical engineering dept., you may want to tap into that resource for a
good EE type person that understands analog/digital interfaces.

Hope this is of some aid.

Peter Tomic

-----Original Message-----
} From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu]
Sent: Wednesday, August 28, 2002 12:58 PM
To: Microscopy-at-sparc5.microscopy.com


Greetings all,

I am looking into getting digital output from a Hitachi SN-2460 SEM.
Has anyone out there converted this particular scope? Any suggestions?

Another idea a colleague and I tossed around was upgrading the EDS
system and using it as a source of digital image files. We currently
have a Noran Voyager system with a Sun Microsystems workstation. I am
sure that a Windows based system is now available-does anyone know if it
is possible, and at what price, to simply buy a new computer and
software package and connect it to the existing detector? Any
suggestions would be welcome.

Sincerely,


Todd

Todd A. Kostman, Ph.D.
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
University of Wisconsin Oshkosh
800 Algoma Blvd
Oshkosh, WI 54901-8640
ph; (920) 424-7301
fax: (920) 424-1101



From daemon Wed Aug 28 22:36:05 2002



From: Ronald Vane :      RVane-at-Evactron.com
Date: Wednesday, August 28, 2002 5:45 PM
Subject: Probe analytical problem

Contents Retrieved from Microscopy Listserver Archives
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Dear Malcolm:

I would start by checking for an unexpected element. Second check your
background correction.

Ronald Vane
XEI Scientific
(Former Xray applications specialist)

-----Original Message-----
} From: Dr Malcolm Roberts {m.roberts-at-ru.ac.za}
To: Microscopy discussion group {Microscopy-at-sparc5.microscopy.com}



From daemon Thu Aug 29 00:13:40 2002



From: Arthur Day :      ard-at-ansto.gov.au
Date: Thu, 29 Aug 2002 15:04:28 +1000
Subject: Re: Analysis Routine

Contents Retrieved from Microscopy Listserver Archives
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Roy,

If you mean analysis of XRD data ask around about a piece of sofware
called Jade. I think it might be able to do these things. It can
certainly import the data in a wide variety of different formats.


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/


From daemon Thu Aug 29 04:37:29 2002



From: Zhang Qixing :      yt-zqx-at-sohu.com
Date: Thu, 29 Aug 2002 17:27:15 +0800
Subject: 12v dc saving energy light

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Please look at our web site:
{http://www.yt-economy.com/senke.htm}
Best regards
Zhang Qixing
address: nantong road no.71 Yantai China
Yantai senke co.ltd
tel:86-0535-6675831
fax:86-0535-6675830
post code:264000
email:yt-zqx-at-sohu.com


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-----------------------------------------------
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From daemon Thu Aug 29 04:37:32 2002



From: Zhang Qixing :      yt-zqx-at-sohu.com
Date: Thu, 29 Aug 2002 17:28:50 +0800
Subject: 12v dc saving energy light

Contents Retrieved from Microscopy Listserver Archives
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Please look at our web site:
{http://www.yt-economy.com/senke.htm}
Best regards
Zhang Qixing
address: nantong road no.71 Yantai China
Yantai senke co.ltd
tel:86-0535-6675831
fax:86-0535-6675830
post code:264000
email:yt-zqx-at-sohu.com


{ {---ÒÔÉÏÓʼþÄÚÈÝÓëÈí¼þ¿ª·¢ÉÌÎÞ¹Ø---} }
-----------------------------------------------
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ÉÌÎñÓÊÏäËÑË÷: ÒÚ»¢EmailËÑË÷´óʦ
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²¡¶¾Óʼþ¿ËÐÇ: ÒÚ»¢Email°²È«´óʦ
......



From daemon Thu Aug 29 06:42:21 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Thu, 29 Aug 2002 09:02:53 -0230
Subject: RE: Probe analytical problem

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Malcolm writes ...

} ...
} ... We've the simple task of
} analysing some olivine crystals for their major (Si, Mg, Fe) and trace
} (Ca, Ni, Mn) elements. using materials for this
} ... However, when we go to the actual
} unknowns, Fe is sytematically about 3 wt % lower than expected for a
} stoichiometric olivine assuming the Mg and Si are ok. ...
} ...
} I considered C coating but thought that this is more likely to affect
} attenuation of the lower energy X-rays such as Si and Mg.
} ...

If you suspect the carbon coating at all, you might suspect charging which
will attenuate Fe more than the lighter elements. I have to admit, olivines
are not known to be a polishing or coating problem, but there may be higher
resistances at grain bountries. What beam current are you using? You might
try something lower than reccommended (e.g., ~ 5nA) just for isolating this
symptom.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From daemon Thu Aug 29 07:36:18 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 29 Aug 2002 07:14:13 -0500
Subject: RE: Probe analytical problem

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One possibility...

} From you post, I infer that you are performing the analysis on uncoated
insulating material at high vacuum. If these assumptions are correct, your
error may be from specimen charging.

One important parameter in quantitation is the incident beam potential (kV).
If your specimen is charging, the potential (kV value) of the charge will
reduce the effective potential of the original beam.

Effective kV = (Initial beam kV) - (specimen surface potential in kV)

The charge induced reduction is often variable and difficult to accommodate
in calculations. The reduced *effective* kV can lower the Fe K lines
response below theoretical for an unaffected beam. Lower energy lines from
lighter elements would be affected less. The result would be lower than
actual/expected values for Fe concentration.

For the best result, although not always practical, the standards and the
specimen should have the same carbon coating thickness/density. This is
often achieved by coating the standards and the unknown at the same time,
adjacent to one another (for even distribution - depends on coater).

Woody White
McDermott Technology Inc.
McD: http://www.mtiresearch.com/
Mine: http://woody.white.home.att.net


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Hallo Folks,
Here's a baffling thing for you to mull over. I'm after some
pointers after scratching my head for a couple of days. Here goes. We
have a JEOL 733 with 4 WD spectrometers. We've the simple task of
analysing some olivine crystals for their major (Si, Mg, Fe) and trace
(Ca, Ni, Mn) elements. This should be a very simple, rudimentary and
stressless thing to do, and normally is. The sad thing is, things aren't
going normally. Standardisation is as simple as possible with as few
standards as possible, and checked rigorously using materials for this
purpose mounted in our standard block. However, when we go to the actual
unknowns, Fe is sytematically about 3 wt % lower than expected for a
stoichiometric olivine assuming the Mg and Si are ok. We can make this
assumption because for an olivine of a specified SiO2 content we would
expect a specified MgO content, and this checks out. For the given FeO
content we would expect lower MgO and SiO2 contents, and this would
simply make the totals worse. I've checked out the hardware and the fact
that the calibration works for unknowns on the standard block suggests
to me that there is no problem with the analytical protocol we're using.
I considered C coating but thought that this is more likely to affect
attenuation of the lower energy X-rays such as Si and Mg.
Has anyone some suggestions on what the cause of this
strange behaviour for a very simple mineral could be?
Thanks,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (try your luck!)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA




From daemon Thu Aug 29 07:55:02 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Thu, 29 Aug 2002 08:44:21 -0400
Subject: RE: New SEM opproximate price

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Scott,
I was only looking for rough estimates.
Here the info that I've received:

*****************************************************************
Without knowing the model numbers of your equipment, it is not possible to
give an exact estimate. I also do not know the seriousness of their
interest.

I would guess the cost to be in the range of $250-400K. It depends on
options as much as anything. I think decent SEMs can be purchased for
around $150K, more if they are fitted with a FE gun. EDS can cost another
$50-100K. I am less familiar with WDS. We had a system that cost $60K for a
single spectrometer. I don't know what current prices are.
********************************************************
Here is my guess

New SEM $100K (base modle)
EDS $50K
WDS $70K
BSE $10K
*************************************************************

Pavel,
You dont say what model or manufacture your microscope is, are you
looking for replacement cost or a brandnew sem with basic features that you
have listed. If the latter I would say a reasonable estimate for a sem with
features you have noted would be in the order of 370K, depending on
manufacturer and stage and chamber requirements,ie 100-120k for wds, -at-60k
for eds, 150k for sem bsd
Regards
Mike Webber
***************************************************************
Hello Pavel,

Since we are a commercial vendor of SEMs and accessories, I thought it
would be more appropriate to answer your question off-line. First, I
hope I am not being out of line by answering you and apologize if so.
I will not try to sell you an SEM! Just give you a straightforward
answer.

We represent (sell and service) SEMs from both CamScan Electron Optics
in the UK, and Tescan, located in Brno, Czech Republic. The full range
of SEMs that these companies manufacture range in price from about
$75,000 list price to about $300,000 list price. The three main factors
which affect the price of an SEM are:

1) Chamber and stage design: Large chamber SEMs are significantly more
expensive, not only because the cost of the larger chamber itself, but
also the cost of the larger vacuum system required to pump it. Stages
can be simple in design, with fewer motions (e.g. X and Y translation
only), or fully eucentric 6-axis designs with automated (motorized)
control.

2) Conventional high vacuum SEM or Variable Pressure SEM (also called
'Low Vacuum' by some, or environmental SEM if it operates at a high
enough pressure to image water stably at room temperature). Variable
pressure SEMs have more complex vacuum system designs and are
therefore more expensive (typically about $10,000 to $20,000 more than
the conventional counterpart).

3) Electron source type: conventional thermionic (filaments are heated
to high temperatures to emit electrons) tungsten hairpin guns are the
cheapest and have the lowest performance in terms of beam brightness,
ultimate resolution, and image S/N ratio. Thermionic LaB6 (Lanthanum
Hexaboride) guns have about 5-10 times the beam brightness of tungsten
and are generally about $10,000-$20,000 more expensive (they also
require better gun vacuum and usually have independent ion pumps and
gun isolation valves). Field Emission electron sources are the highest
performance and these SEMs are significantly more expensive than the
thermionic types. FESEMs have 100 times the brightness of tungsten
guns, very good ultimate resolution, and very high image S/N. If you
must work regularly at high magnifications (e.g. 50,000X to 100,000x
or higher), especially at low kV's, then a FESEM is for you.

Adding a backscattered electron detector to a SEM will generally cost
about $7500 to $15,000 depending on the detector type and vendor. Most
variable pressure SEMs should include this detector since a
conventional Everhart-Thornley secondary electron detector is unusable
in the vacuum range of VPSEMs. You can also buy special low vacuum
secondary electron detectors from most vendors which are capable of
providing SE images in variable pressure mode - they are usually about
$12,000 to $15,000.

To finish the answer, EDS systems are almost always in the range of
$40,000 to $80,000 for new systems with light element detectors. For
$50,000 or less, you can choose from among many commercial systems
which will provide good energy resolution, qualitative elemental
analysis, quantitative elemental analysis, and digital Xray
mapping/line profile capabilities (many provide full spectrum mapping
for this price). WDS systems are more complex and therefore more
expensive, usually in the range of $120,000 or so for a full-range
spectrometer (Noran sells a smallerand less expensive spectrometer
which is optimized for only a few elements, which you can choose).

You can probably improve on the separate EDS/WDS prices by buying an
combined or integrated system, available from some of the companies
(e.g. Oxford Instruments, Noran, and I believe Edax has just
introduced a WDS system).

I hope this has helped some. If you wish, please feel free to contact
me offline if you have more questions.
------------------------------------------------------------------

} One of mine coworkers would like to find out the opprox. price for new SEM
} with same features as mine.
} EDS/WDS, backscattered detector, dot mapping, line profile.


Best regards,

Tony mailto:towens-at-camscan-usa.com



Tony Owens
CamScan USA Inc.
508 Thomson Park Dr.
Cranberry Twp., PA 16066
Tel: (724) 772-7433
Fax: (724) 772-7434
URL: www.camscan-usa.com
***********************************************************************
Pavel -

You need to be more specific. The range
is quite large. Is your gun W, LaB6, FEG, SFEG?

Load lock?
Polaroid/Hi-Res monitor?
System volume?
Low Vac, ESEM, Enviro?
Photo-printer?
3, 4, 5, or 6-axis stage?
Motorized?

etc......

All of these can add large amounts of $.

JQ
**********************************************************************
Between used and new, the cost could range between $1 to
$500K,. not including shipping. Your post is rather ambiguous
relative to exactly what you are looking for. Is this a SEM or FESEM?
Big difference. Turbo or diff pump?, etc., etc., blah, blah.


gg




From daemon Thu Aug 29 08:31:55 2002



From: sstouden-at-thelinks.com
Date: Thu, 29 Aug 2002 08:25:06 -0500 (CDT)
Subject: Re: Macrophages

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were they changing in refractive index?


On Tue, 27 Aug 2002, Emmanuelle Roux wrote:

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} -----------------------------------------------------------------------.
}
}
} Hi Leslie,
} It will look like a silly question but have you looked at your "invisible pellet" under a microscope or just by looking at your tube and not seeing it? I remember working with cultured macrophages and while doing an immunolabeling "loosing" my sample (I could not see a pellet anymore at some point of the experiment). But after putting the "invisible pellet" on a slide (cytospots) and looking under a microscope the cells were there! For some reason they became invisible for the eyes but were still there.
} Emmanuelle
}
} Emmanuelle Roux, PhD
} Senior Scientist
} Caprion Pharmaceuticals
} 7150 Alexander Fleming
} St-Laurent, H4S 2C8
} Quebec, Canada
} Tel: 514-940-3600 ext. 3773
} Fax: 514-940-3620
}
}



From daemon Thu Aug 29 09:17:52 2002



From: Germinario, L T - Eastman :      germ-at-eastman.com
Date: Thu, 29 Aug 2002 09:41:09 -0400
Subject: Microscopist Opening: Eastman Chemical Company

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Job Posting:

Microscopist Opening

Eastman Chemical Company has a position in its corporate research and
development labs for a microscopist. Extensive experience with both optical
and electron microscopy is required and experience in AFM, computer
programming, and/or particle size analysis would be a strong plus.
Knowledge of polymer morphology and/or the morphology of coatings and inks
is also highly desirable. A PhD is preferred. The laboratory is well
equipped with several optical microscopes, an SEM, a TEM, an AFM, and a
particle size analyzer. The successful candidate will be working with this
equipment and other scientists and engineers at Eastman to develop
new/improved polymers and chemicals and to help solve manufacturing
problems. Candidates must be highly motivated, have good communication
skills and be able to work with teams.

Eastman Chemical Company, a Fortune 500 company, is a major manufacturer of
plastics, fibers, and specialty organic chemicals. This position is at the
Kingsport, TN site. Kingsport is located in northeast Tennessee in the
foothills of the Smoky Mountains. It is Eastman's policy to provide equal
opportunity for all qualified persons; to prohibit unlawful discrimination
in employment practices, compensation practices, personnel procedures, and
the administration of benefit plans and other programs; and to promote a
full realization of equal employment opportunity through continuing
affirmative action throughout company establishments. Reference Requisition
No. 723 in your cover letter. Send CV/Resume to lmotz-at-eastman.com
{mailto:lmotz-at-eastman.com} or to Eastman Chemical Company, PO Box 1975,
B-215 - Staffing, Attn: Laurie Motz, Kingsport, TN 37662.


} Louis T. Germinario (Lou)
} Physical Chemistry Research Laboratory
} Eastman Chemical Company
} Lincoln Street, B-150B
} P.O. Box 1972
} Kingsport, TN 37662-5150
} (423) 229-4047
} (423) 229-4558 (Fax)
} mailto:germ-at-eastman.com
}
}


From daemon Thu Aug 29 09:50:48 2002



From: Beth Gregory :      gregory-at-4pi.com
Date: Thu, 29 Aug 2002 10:40:38 -0400
Subject: RE: conversion of SN-2460 to digital

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Peter,
I don't believe the original message is proposing the method you used. Many
companies (including my own) provide EDX/imaging upgrades that replace the
EDX system except for the detector and detector preamp. On most detectors,
these upgrades are very easy to install.

Regarding the imaging, older SEMs vary even within the same model number.
The Hitachi S570 is sometimes equipped to readily accept an active-scan
system. We've seen others that require the installation of a Hitachi
digital beam control box. With an active-scan system, older SEMs can
acquire high-resolution digital images of amazing quality. Prices vary
greatly and typically depend on the software requirements (i.e. acquisition
and analysis, versus just acquisition).

Beth Gregory
4pi Analysis, Inc.

} Todd;
}
} I have done as you mentioned on a Hitachi S570. That is, used the EDX
} system's imaging and beam control as a capture vehicle. The only issue at
} the time was the resolution of the captured image relative to acquisition
} time since the processing capability was based on an old microprocessor and
} motherboard in the EDX hardware. In the older SEMs such as the Hitachi
} S570, there is no readily available port to grab the horizontal and vertical
} scan information and hence the video board had to be physically spliced into
} and the electronic levels made compatible with the EDX electronics. It was
} a messy affair but ultimately did work. The system worked on a Win95
} platform.
}
} The cost to do this on your particular microscope will likely be a function
} of who will be doing it, EDX vendor or graduate student. If you have an
} electrical engineering dept., you may want to tap into that resource for a
} good EE type person that understands analog/digital interfaces.
}
} Hope this is of some aid.
}
} Peter Tomic
}
} -----Original Message-----
} } From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu]
} Sent: Wednesday, August 28, 2002 12:58 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: conversion of SN-2460 to digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Thu Aug 29 10:31:04 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Thu, 29 Aug 2002 11:13:19 -0400
Subject: Re: TEM - macrophage collection

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You can also try growing them on a 100 mm plate. I have done this after the
osmification
and using a rubber cell scraper, gently removed enough cells to get a visible
pellet. Also check
the plate under phase microscopy to ensure cell numbers.

Mike D.

"Sherwood, Margaret" wrote:

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} -----------------------------------------------------------------------.
}
} Have you thought about growing them on a coverslip and then processsing the
} cells intact--the final stage would involve inverting a beem capsule with
} epon on top of it, polymerizing it, and then using liquid nitrogen to pop
} the capsul off. Cells adhere nicely to the epon and then you can section.
}
} Peggy Sherwood
} Lab Associate, Photopathology
} Wellman Laboratories of Photomedicine (W224)
} Massachusetts General Hospital
} 55 Fruit Street
} Boston, MA 02114
} 617-724-4839 (voice mail)
} 617-726-6983 (lab)
} 617-726-3192 (fax)
} msherwood-at-partners.org
}
} } -----Original Message-----
} } From: Leslie Cummins [SMTP:gunther-at-aecom.yu.edu]
} } Sent: Monday, August 26, 2002 3:01 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: TEM - macrophage collection
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello Listers
} }
} } I am having a problem attempting to scrape and collect primary macrophage
} } grown on a 60mm plastic petri dish.
} }
} } The cells are fixed in 4% paraformaldehyde, blocked with 0.05M glycine,
} } buffer rinsed, then water rinsed. I am using a disposable cell scraper
} } from Fisher and scraping them in a very small amount of water, almost
} } dry. When I collect the water with a pipet and transfer to an eppendof
} } tube to spin the pellet down, there seem to be no cells. I have tried
} } spinning them very hard, or very long but to no avail. I use this
} } protocol
} } on other cell types and get wonderful pellets, with plenty of sample to
} } work with.
} }
} } It seems to be cell type specific and I was wondering if anyone had any
} } ideas to help me get a usable, visible pellet. I am trying to do
} } ultrathin
} } cryosections, so I am slightly limited in my options.
} }
} } Thanks in advance.
} } Leslie
} }
} }
} }
} } Leslie Gunther Cummins
} } Analytical Imaging Facility
} } Albert Einstein College of Medicine
} } 1300 Morris Park Ave.
} } Bronx, NY 10461
} } 718-430-3547
} }
} } http://www.aecom.yu.edu/aif/
} }



From daemon Thu Aug 29 12:25:50 2002



From: LT4725n42-at-aol.com
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From: LT4725n42-at-aol.com
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Subject: Join The Fun ....

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Amazing New Work-At-Home Plan.
Guarantees $50 Per Hour In Your Spare Time.

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Just Follow Directions And Get Paid.

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From daemon Thu Aug 29 14:22:11 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 29 Aug 2002 13:08:00 -0600
Subject: RE: conversion of SN-2460 to digital

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Beth, all,

Considering the fact that the SEM already has an EDS system attached, I
would guess, that it is already equipped with the digital beam control
interface. That would probably allow to hook up any of the digital
acquisition systems, yours, ours, etc.

A possibly more cost efficient way might be to talk to Noran and see if they
have a migration possibility from this Unix system to a PC based system.
Noran is using our software on some of their systems, so that might be a
possibility to get image acquisition on a PC and some processing
capabilities at the same time. However, depending on the age of the system,
that might not be possible, or only with additional hardware changes.

If only image acquisition is required, our ADDA II can be operated in
parallel to an existing EDS system (I am sure your system can do that also),
with options do to dot mapping.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

******* Disclaimer ********
As the manufacturer of digital image acquisition systems we DO have an
interest (financial and otherwise) in the products mentioned above. Please
make sure to get all information before making a decision.
**************************


-----Original Message-----
} From: Beth Gregory [mailto:gregory-at-4pi.com]
Sent: Thursday, August 29, 2002 8:41 AM
To: Microscopy-at-sparc5.microscopy.com


Peter,
I don't believe the original message is proposing the method you used. Many
companies (including my own) provide EDX/imaging upgrades that replace the
EDX system except for the detector and detector preamp. On most detectors,
these upgrades are very easy to install.

Regarding the imaging, older SEMs vary even within the same model number.
The Hitachi S570 is sometimes equipped to readily accept an active-scan
system. We've seen others that require the installation of a Hitachi
digital beam control box. With an active-scan system, older SEMs can
acquire high-resolution digital images of amazing quality. Prices vary
greatly and typically depend on the software requirements (i.e. acquisition
and analysis, versus just acquisition).

Beth Gregory
4pi Analysis, Inc.

} Todd;
}
} I have done as you mentioned on a Hitachi S570. That is, used the EDX
} system's imaging and beam control as a capture vehicle. The only issue at
} the time was the resolution of the captured image relative to acquisition
} time since the processing capability was based on an old microprocessor and
} motherboard in the EDX hardware. In the older SEMs such as the Hitachi
} S570, there is no readily available port to grab the horizontal and
vertical
} scan information and hence the video board had to be physically spliced
into
} and the electronic levels made compatible with the EDX electronics. It was
} a messy affair but ultimately did work. The system worked on a Win95
} platform.
}
} The cost to do this on your particular microscope will likely be a function
} of who will be doing it, EDX vendor or graduate student. If you have an
} electrical engineering dept., you may want to tap into that resource for a
} good EE type person that understands analog/digital interfaces.
}
} Hope this is of some aid.
}
} Peter Tomic
}
} -----Original Message-----
} } From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu]
} Sent: Wednesday, August 28, 2002 12:58 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: conversion of SN-2460 to digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Thu Aug 29 15:03:22 2002



From: cbc-at-post.queensu.ca ()
Date: Thu, 29 Aug 2002 14:52:30 -0500
Subject: Ask-A-Microscopist:what is Dr. Vogel's Sparbeiz

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cbc-at-post.queensu.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
August 29, 2002 at 11:02:32
---------------------------------------------------------------------------

Email: cbc-at-post.queensu.ca
Name: Charlie Cooney

Organization: Queens University

Education: Graduate College

Location: Kingston, Ontario Canada

Question: I have been doing some housekeeping and came
across a bottle of Dr. Vogel's Sparbeize. My
recollection is that this was an additive to
an electropolishing solution for metals but I
cannot remember what the chemical compostion of
this stuff is or what solution it was use in.
Does anyone know how to use this stuff and what
the compositon is?

Thanks


---------------------------------------------------------------------------


From daemon Thu Aug 29 17:06:26 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Thu, 29 Aug 2002 16:57:48 -0500
Subject: Adhesive for binding plastic serial sections

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Listies

I need to find a good adhesive to use as for plastic serial sections. One
recommendation was a product called Pattex diluted with xylene. I am unable
to locate this product.

Any other suggestions for accomplishing good serial sections? I'm using a
diamond histo knife with big boat.

Thanks,
Tim Quinn
University of Kansas
Program Assistant/Microscopists
Ornithology Dept.
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556/785-331-4107
tquinn-at-ku.edu



From daemon Thu Aug 29 18:45:33 2002



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au (by way of
Date: Thu, 29 Aug 2002 18:33:29 -0500
Subject: Sparbeize

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Charlie.

Sparbeize is indeed an additive to other etching solutions. It was
produced by a German company. (Max Hoeck o.H.G. in Duesseldorf).
It was used for etching mainly stainless steel samples with a high
Cr content. eg.
10 ccs HNO3
0.30 Vogels Sparbeize
100 ccs HCL
100 ccs dist H2O
Etching either at room temp or 50 degrees C.
As I can remember, the actual ingredients of Sparbeize were kept secret.

Cheers

Hans Brinkies
Professional Officer, Electron Microscopy and Metallography
Swinburne, University of Technology
School of Engineering and Science
Industrial Microscopy Laboratory
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au


From daemon Thu Aug 29 19:23:57 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Thu, 29 Aug 2002 17:15:36 -0700
Subject: Re: Occupational injury?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to everybody who replied to my question. I started off answering each
reply individually, but there are just so many that I hope everybody will
accept this blanket gratitude. It seems to be a common problem among EM
people and other microscopists, so perhaps it could count as an occupational
injury. If it is, please everybody, take care to prevent it, as far as you
can.

Lesley Weston



From daemon Thu Aug 29 20:32:00 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 29 Aug 2002 18:25:08 -0700
Subject: Re: New SEM opproximate price

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Excellent!! what did you recommend for the purchase?

gg

At 04:07 AM 8/27/2002, you wrote:

} I appreciate the information. I think, I've got more than enough.
} Thank you all very much for comprehensive answers.
}
} Pavel



From daemon Fri Aug 30 02:33:02 2002



From: Gilles Hug :      gilles.hug-at-onera.fr
Date: Fri, 30 Aug 2002 12:20:41 +0200
Subject: Re: Probe analytical problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

pattex is a subdivision of Henkel / Germany. - I do not know if their
products are available outside of Germany ...
Take a look at their website http://www.pattex.de, probably the
"Kraftkleber" is the one you are looking for?

Greetings
Gunnar


} From: "Quinn, Tim Lee" {tquinn-at-ku.edu}
To: "'Microscopy-at-MSA.MIcroscopy.com'"
{Microscopy-at-sparc5.microscopy.com}



Hi,
You might want to check this paper:
3A critical evaluation of the results of the '92 round robin
microanalysis test(EDS & Peels) performed by the Ile de Frande
TEMnetwork, in MMM 4, p387-399. (multiple authors)
Analysis protocols were discussed in the instance of Olivine. If you
want a sample we usedyou should contact J. Ingrin (he is now somewher
in Toulouse).
Gilles

}
} Hallo Folks,
} Here's a baffling thing for you to mull over. I'm after some
} pointers after scratching my head for a couple of days. Here goes. We
} have a JEOL 733 with 4 WD spectrometers. We've the simple task of
} analysing some olivine crystals for their major (Si, Mg, Fe) and trace
} (Ca, Ni, Mn) elements. This should be a very simple, rudimentary and
} stressless thing to do, and normally is. The sad thing is, things aren't
} going normally. Standardisation is as simple as possible with as few
} standards as possible, and checked rigorously using materials for this
} purpose mounted in our standard block. However, when we go to the actual
} unknowns, Fe is sytematically about 3 wt % lower than expected for a
} stoichiometric olivine assuming the Mg and Si are ok. We can make this
} assumption because for an olivine of a specified SiO2 content we would
} expect a specified MgO content, and this checks out. For the given FeO
} content we would expect lower MgO and SiO2 contents, and this would
} simply make the totals worse. I've checked out the hardware and the fact
} that the calibration works for unknowns on the standard block suggests
} to me that there is no problem with the analytical protocol we're using.
} I considered C coating but thought that this is more likely to affect
} attenuation of the lower energy X-rays such as Si and Mg.
} Has anyone some suggestions on what the cause of this
} strange behaviour for a very simple mineral could be?
} Thanks,
} Malc.
}
} --
} Dr MP Roberts Phone: [+27](0)46 603 8313
} Dept of Geology Fax: [+27](0)46 622 9715
} Rhodes University Cell: 083 4060 262 (try your luck!)
} 6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
} SOUTH AFRICA


--
_______________________________________________________
Gilles Hug
LEM, UMR 104, ONERA-CNRS, BP72
92322 Chatillon Cedex, France
tel : +33 1 46 73 45 42 fax : +33 1 46 73 41 55
mailto:Gilles.Hug-at-onera.fr
http://www.onera.fr/lem/anachistr/index.html
_______________________________________________________
Office National d'Etudes et de Recherches Aerospatiales
------- & ------
Centre National de la Recherche Scientifique
_______________________________________________________


From daemon Fri Aug 30 07:04:53 2002



From: Ping Li :      pli-at-dal.ca
Date: Fri, 30 Aug 2002 08:55:59 -0300
Subject: Re: LF shielded computer monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could some of you please provide me some information regarding where to
purchase a 21" or larger low frequency shielded computer monitor? I would
like to buy one but having trouble to find a supplier. The monitor will be
used to replace the smaller one on our Tecnai TEM. Your help is greatly
appreciated.

Thank you,
Ping Li

--
Ping Li, Ph.D.
Director, Scientific Imaging Suite
Department of Biology
Dalhousie University
Halifax, NS B3H 4J1
Canada

Tel: 902-494-3309
Fax: 902-494-3736
E-mail: Ping.Li-at-Dal.Ca




From daemon Fri Aug 30 07:30:40 2002



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Fri, 30 Aug 2002 08:41:02 -0400
Subject: Mystery Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was not looking for purchase! Just the info


----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "ATC SEM Laboratory" {atcsem-at-earthlink.net}
Cc: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
Sent: Thursday, August 29, 2002 9:25 PM


Hi Everyone,

We got a brief protocol that listed a resin for TEM called
LX-112. Has anyone ever heard of it? Does anyone have a protocol?

Thanks!

Lesley



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Fri Aug 30 09:55:52 2002



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 30 Aug 2002 09:46:46 -0500 (CDT)
Subject: Re: Adhesive for binding plastic serial sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To adhere serial setions together into a ribbon while sectioning, I'v used
(with success) a small amount of a product called Tackiwax can be applied
to the sides of the blockface at the top and bottom of the trapezoid. I
think the reference is in M.A. Hayat's book on EM Techniques for
Biological Electron Microscopy.

I ordered a tub (1 lb. container - a lifetime supply for ultramicrotomy)
from Fisher Scientific, but other suppliers may be able to get it for you.
Here's information off of the label:

Boeker Tackiwax
Cat. No. 11444000
Boekel Scientific
855 Pennsylvania Blvd.
Feasterville, PA 19053

Heather Owen


Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall, P.O. Box 413
Milwaukee, WI 53210
USA

Phone: (414)229-6816



From daemon Fri Aug 30 10:33:48 2002



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Fri, 30 Aug 2002 11:22:42 -0400
Subject: Re: Mystery Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ladd makes it. It's very nice when using plastic culture dishes. I use the
same recipe as for epon 812. Let me know if you need specifics.
Mary Gail Engle

At 08:41 AM 8/30/02 -0400, Lesley S. Bechtold wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-9700


From daemon Fri Aug 30 11:03:07 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 30 Aug 2002 08:55:22 -0700
Subject: Re: LF shielded computer monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Sony Multiscan E500 (21") and most all of the Multiscan
series are shielded.

A better course might be to look into 20" flat panel LCDs.
I'm using a 20" RGB+sync unit made in Germany with
a NEC LCD display unit. It works quite well. NEC also
makes some 18" LCD displays that are very nice. I'm
not sure if they make larger ones. The 18" one I
have used is the 1850 and is the standard LCD display
on the FEI Sirion FEGSEM.

gary


At 04:55 AM 8/30/2002, you wrote:

} Could some of you please provide me some information regarding where to
} purchase a 21" or larger low frequency shielded computer monitor? I would
} like to buy one but having trouble to find a supplier. The monitor will be
} used to replace the smaller one on our Tecnai TEM. Your help is greatly
} appreciated.
}
} Thank you,
} Ping Li
}
} --
} Ping Li, Ph.D.
} Director, Scientific Imaging Suite
} Department of Biology
} Dalhousie University
} Halifax, NS B3H 4J1
} Canada
}
} Tel: 902-494-3309
} Fax: 902-494-3736
} E-mail: Ping.Li-at-Dal.Ca



From daemon Fri Aug 30 11:26:02 2002



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Fri, 30 Aug 2002 12:20:15 -0400
Subject: Re: Mystery Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lesley,

LX-112 is the EPON replacement sold by LADD. We use is routinely and
follow the traditional Luft formulations.

Frank

At 08:41 AM 8/30/02 -0400, Lesley S. Bechtold wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Aug 30 13:14:33 2002



From: robert.fowler-at-tdktca.com
Date: Fri, 30 Aug 2002 14:10:05 -0400
Subject: salary survey

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi all
I am researching salaries. The problem I am having is my job is primarily
failure analysis, but I am a technician so most surveys list quality
technician and quality engineer. I have responsibilities in both areas. Any
help off or online would be most appreciated. 9 years experience.

Apologies for off topic discussion

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com


THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY
TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL
INFORMATION.

If you are not the intended recipient, be advised that any use,
dissemination, distribution or duplication of this transmission is strictly
prohibited. If you received this transmission in error, please notify the
sender immediately by electronic reply to this transmission or by phone
(847-803-6100). Thank you.




From daemon Fri Aug 30 15:42:25 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 30 Aug 2002 18:22:12 -0700
Subject: Re: New SEM opproximate price

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lesley,

LX-112 is a Ladd Research product. It is used in wide variety of projects
from EM research to the aerospace industry.
Please reply directly with more details on your project, check our web site,
http://www.laddresearch.com, or call the number listed below and ask for Dr.
Charles Duvic our chemist and you can discuss it with him.

Thank you,

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Lesley S. Bechtold" {lsb-at-jax.org}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 30, 2002 8:41 AM


Ah....OK. Can you tell us what the basic premise was
for doing this? No condemnation, just wondering why
you are doing this exercise. As you can imagine,
there is a huge range of options, systems and prices.

I'm a cat....curious.

gary g.

At 05:22 AM 8/30/2002, you wrote:
} I was not looking for purchase! Just the info
}
}
} ----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
} To: "ATC SEM Laboratory" {atcsem-at-earthlink.net}
} Cc: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
} Sent: Thursday, August 29, 2002 9:25 PM
} Subject: Re: New SEM opproximate price
}
}
} } Excellent!! what did you recommend for the purchase?
} }
} } gg
} }
} } At 04:07 AM 8/27/2002, you wrote:
} }
} } } I appreciate the information. I think, I've got more than enough.
} } } Thank you all very much for comprehensive answers.
} } }
} } } Pavel
} }



From daemon Sat Aug 31 08:47:15 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 31 Aug 2002 09:35:50 -0500
Subject: Tackiwax vs. dental wax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Heather A. Owen wrote:
============================================================

To adhere serial setions together into a ribbon while sectioning, I'v used
(with success) a small amount of a product called Tackiwax can be applied to
the sides of the blockface at the top and bottom of the trapezoid. I think
the reference is in M.A. Hayat's book on EM Techniques for Biological
Electron Microscopy.

I ordered a tub (1 lb. container - a lifetime supply for ultramicrotomy)
from Fisher Scientific, but other suppliers may be able to get it for you.
Here's information off of the label:

Boeker Tackiwax
Cat. No. 11444000
Boekel Scientific
855 Pennsylvania Blvd.
Feasterville, PA 19053

==============================================================
Could someone explain how this product Tackiwax™ apparently a product of
Boekel Scientific would differ from a good dental wax, such as on URL
www.2spi.com/catalog/knives/cavex-set-up-dental-wax-sh.shtml

which is also used pretty widely in the world for ultramicrotomy
applications.

I am not suggesting that this common (but very high quality) dental wax is
better for this application, but it is far more readily available from a
large number of suppliers (such as one of our many competitors like Ted
Pella, Inc.). The Cavex® brand of dental wax, unlike a lot of the dental
wax products on the market, is actually approved for use in dentistry in
most countries around the world. What is not clear to me is whether soft,
medium or hard would be the preferred hardness for this particular
application (to adhere serial sections into a ribbon).

The cost to place a small order for a single item, for many customers, can
be staggering, so I ask this question because if it is not any different
than a good dental wax, such as the Cavex wax, it could keep people in
certain countries from placing a small order (because the shipping and other
costs of importation may be many times larger than the fob value of the item
itself). This is inherently a low cost item and as was pointed out
previously, one pack can last virtually a life time.

Disclaimer: SPI Supplies is a major supplier of high quality dental wax for
use in microtomy applications worldwide so naturally we would have a vested
interest in having customers order this wax from SPI along with their other
needed items.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sat Aug 31 19:50:48 2002



From: curari-at-asu.edu
Date: Sat, 31 Aug 2002 17:36:16 -0700 (MST)
Subject: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers

I am looking for a replacement part for our poloron sputter/coater. It
is a model 0E5000, manufacturing date unknown. The part that is broken is a
hollow glass cylinder that has a rubber seal at either end and acts as the
sputtering chamber. Any recommendations on a supplier or glass manufacturer is
welcomed or other ideas! Thank you in advance.

Wil Kunkel
curari-at-asu.edu
student of chemistry
This email was sent with 100.00% recycled electrons.


From daemon Sat Aug 31 22:56:59 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 31 Aug 2002 20:49:33 -0700
Subject: Re: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Polaron (check spelling) supplier in the US should
be able to get this item for you. If not, that would be a
good reason to avoid this brand of coater.

Why did it fail? This seems to me to be a very low
failure rate item. The rubber boots do need to be
replaced at some interval (like on my Anatech Hummer
VII). But having the glass cylinder crack would be really
disturbing to me. Did you miss-treat it?

gary g.


At 05:36 PM 8/31/2002, you wrote:

} Dear Listers
}
} I am looking for a replacement part for our poloron
} sputter/coater. It
} is a model 0E5000, manufacturing date unknown. The part that is broken is a
} hollow glass cylinder that has a rubber seal at either end and acts as the
} sputtering chamber. Any recommendations on a supplier or glass
} manufacturer is
} welcomed or other ideas! Thank you in advance.
}
} Wil Kunkel
} curari-at-asu.edu
} student of chemistry
} This email was sent with 100.00% recycled electrons.



From daemon Sun Sep 1 04:53:12 2002



From: Zhang Qixing :      yt-zqx-at-sohu.com
Date: Sun, 1 Sep 2002 17:43:22 +0800
Subject: 12v dc saving energy light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

solar lamps
Please look at our web site:
{http://www.yt-economy.com/senke.htm}
Best regards
Zhang Qixing
address: nantong road no.71 Yantai China
Yantai senke co.ltd
tel:86-0535-6675831
fax:86-0535-6675830
post code:264000
email:yt-zqx-at-sohu.com


{ {---ÒÔÉÏÓʼþÄÚÈÝÓëÈí¼þ¿ª·¢ÉÌÎÞ¹Ø---} }
-----------------------------------------------
»¶Ó­Ê¹ÓÃÒÚ»¢EmailϵÁÐÈí¼þ
ÏÂÔصØÖ·: http://www.ehoosoft.com
ÉÌÎñÓÊÏäËÑË÷: ÒÚ»¢EmailËÑË÷´óʦ
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²¡¶¾Óʼþ¿ËÐÇ: ÒÚ»¢Email°²È«´óʦ
......


From daemon Sun Sep 1 04:53:12 2002



From: Zhang Qixing :      yt-zqx-at-sohu.com
Date: Sun, 1 Sep 2002 17:43:01 +0800
Subject: 12v dc saving energy light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


solar lamps
Please look at our web site:
{http://www.yt-economy.com/senke.htm}
Best regards
Zhang Qixing
address: nantong road no.71 Yantai China
Yantai senke co.ltd
tel:86-0535-6675831
fax:86-0535-6675830
post code:264000
email:yt-zqx-at-sohu.com


{ {---ÒÔÉÏÓʼþÄÚÈÝÓëÈí¼þ¿ª·¢ÉÌÎÞ¹Ø---} }
-----------------------------------------------
»¶Ó­Ê¹ÓÃÒÚ»¢EmailϵÁÐÈí¼þ
ÏÂÔصØÖ·: http://www.ehoosoft.com
ÉÌÎñÓÊÏäËÑË÷: ÒÚ»¢EmailËÑË÷´óʦ
ÓʼþȺ·¢ÌØ¿ì: ÒÚ»¢EmailÓʲî
²¡¶¾Óʼþ¿ËÐÇ: ÒÚ»¢Email°²È«´óʦ
......



From daemon Sun Sep 1 04:53:13 2002



From: Zhang Qixing :      yt-zqx-at-sohu.com
Date: Sun, 1 Sep 2002 17:42:47 +0800
Subject: 12v dc saving energy light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


solar lamps
Please look at our web site:
{http://www.yt-economy.com/senke.htm}
Best regards
Zhang Qixing
address: nantong road no.71 Yantai China
Yantai senke co.ltd
tel:86-0535-6675831
fax:86-0535-6675830
post code:264000
email:yt-zqx-at-sohu.com


{ {---ÒÔÉÏÓʼþÄÚÈÝÓëÈí¼þ¿ª·¢ÉÌÎÞ¹Ø---} }
-----------------------------------------------
»¶Ó­Ê¹ÓÃÒÚ»¢EmailϵÁÐÈí¼þ
ÏÂÔصØÖ·: http://www.ehoosoft.com
ÉÌÎñÓÊÏäËÑË÷: ÒÚ»¢EmailËÑË÷´óʦ
ÓʼþȺ·¢ÌØ¿ì: ÒÚ»¢EmailÓʲî
²¡¶¾Óʼþ¿ËÐÇ: ÒÚ»¢Email°²È«´óʦ
......



From daemon Sun Sep 1 11:03:53 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Sun, 01 Sep 2002 10:53:47 -0500
Subject: Re: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wil,

I recently had the same problem, and I went to the university's glass
shop in the chemistry department. Even though the size I needed was a
special order item, it still only cost $16, instead of the $120 the
sputter-coater company wanted.
Yours would cost more, since you would need a groove for the o-ring
ground into it, but you'd still save a lot of money.
If your department doesn't have a glass shop anyway (a lot of
university bean counters have closed the glass shops), then try the
big EM facility in mat sci and engineering (I forget the exact
department/institute, sorry, but someone on the list will know) --
they have a good-sized machine shop and might be able to make the
chamber.

Phil

} Dear Listers
}
} I am looking for a replacement part for our poloron sputter/coater. It
} is a model 0E5000, manufacturing date unknown. The part that is broken is a
} hollow glass cylinder that has a rubber seal at either end and acts as the
} sputtering chamber. Any recommendations on a supplier or glass
} manufacturer is
} welcomed or other ideas! Thank you in advance.
}
} Wil Kunkel
} curari-at-asu.edu
} student of chemistry
} This email was sent with 100.00% recycled electrons.

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Sun Sep 1 22:38:56 2002



From: curari-at-asu.edu
Date: Sun, 01 Sep 2002 20:24:02 -0700 (MST)
Subject: Re: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you everyone for the advice and help.

Wil

This email was sent with 100.00% recycled electrons.

On Sun, 1 Sep 2002, Philip Oshel wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Wil,
}
} I recently had the same problem, and I went to the university's glass
} shop in the chemistry department. Even though the size I needed was a
} special order item, it still only cost $16, instead of the $120 the
} sputter-coater company wanted.
} Yours would cost more, since you would need a groove for the o-ring
} ground into it, but you'd still save a lot of money.
} If your department doesn't have a glass shop anyway (a lot of
} university bean counters have closed the glass shops), then try the
} big EM facility in mat sci and engineering (I forget the exact
} department/institute, sorry, but someone on the list will know) --
} they have a good-sized machine shop and might be able to make the
} chamber.
}
} Phil
}
} } Dear Listers
} }
} } I am looking for a replacement part for our poloron sputter/coater. It
} } is a model 0E5000, manufacturing date unknown. The part that is broken is a
} } hollow glass cylinder that has a rubber seal at either end and acts as the
} } sputtering chamber. Any recommendations on a supplier or glass
} } manufacturer is
} } welcomed or other ideas! Thank you in advance.
} }
} } Wil Kunkel
} } curari-at-asu.edu
} } student of chemistry
} } This email was sent with 100.00% recycled electrons.
}
} --
} }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
} Philip Oshel
} Supervisor, AMFSC and BBPIC microscopy facilities
} Department of Animal Sciences
} University of Wisconsin
} 1675 Observatory Drive
} Madison, WI 53706 - 1284
} voice: (608) 263-4162
} fax: (608) 262-5157 (dept. fax)
}
}



From daemon Mon Sep 2 02:26:11 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 02 Sep 2002 00:17:30 -0700
Subject: Re: LF shielded computer monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Follow-up on shielded computer monitor.

These are the specs for my out of production E500:

http://www.ita.sel.sony.com/support/displays/legacy/specs/cpde500.pdf

I also use Sony Multiscan sfII shielded monitors on PCs and Macs.

Look at Emission/EMI and ELF/VLF specs for prospective units.

This is their new 21" monitor:
http://www.sonystyle.com/home/item.jsp?hierc=9683&itemid=8241

This is their 24" monitor (100 pounds--heavy!!):
http://www.sonystyle.com/home/item.jsp?hierc=9683&catid=9710&itemid=6971

These are shielded.

NEC makes good monitors too. Many of them are also shielded.
Check before you buy.

gary

At 04:55 AM 8/30/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Sep 2 17:12:32 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 2 Sep 2002 17:00:41 -0500
Subject: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A faculty member needs to produce some extremely high resolution 24 x
35 mm negatives or masks to demonstrate the diffraction phenomenon
and FFT. He has generated around 24 PostScript files that consist of
a "unit cell" (such as an Escher figure) that is repeated numerous
times to mimic a crystalline lattice. The goal is to project a laser
through the negative masks and generate diffraction patterns as part
of the demonstration.

We have attempted to generate hi res 35 mm negs by means of a
LaserGraphics 8,000 line film recorder but the resolution is
inadequate. He needs the equivalent of 16,000 x 24,000 pixels in the
24 x 35 mm area. This is about 17,000 ppi!

Any suggestions? Any other practical ways to produce these masks?

I had considered micro-lithography but do not have access to the technology.

Many thanks,

John B.




##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Sep 3 03:11:31 2002



From: =?iso-8859-1?Q?Krzysztof_Jan_H=FCbner?= :      hubner-at-IOd.krakow.pl
Date: Tue, 3 Sep 2002 09:47:17 +0200
Subject: Fw: MIRAGE 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John
Sounds too demanding for a demonstration.
Is it not possible to get the same value from the demonstration by
using a less complex image, with lower definition and fewer repeats?
Chris

----- Original Message -----
} From: "John J. Bozzola" {bozzola-at-siu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, September 02, 2002 11:00 PM





} } Dear colleagues and friends,
} }
} } please find below the ascii version of the first Call for Paper of the
}
} } conference MIRAGE'2003; please feel free to distribute it and to
} advertise.
} }
} } The website of the conference is http://telin.rug.ac.be/mirage2003
} }
} } Looking forward to meeting you in Rocquencourt,
} }
} } Jacques Blanc-Talon and Andre Gagalowicz
} }
} } } ---
} }
} } MIRAGE 2003
} }
} } Call for Papers
} }
} } Computer Vision / Computer Graphics
} } Collaboration for
} } Model-based Imaging,
} } Rendering,
} } image Analysis and
} } Graphical special Effects
} }
} } 10-12 March 2003
} } INRIA Rocquencourt, France
} }
} } MIRAGE 2003 is designed as a very peculiar conference in the field of
}
} } Information Technology: it is dedicated to computer vision and
}
} } computer graphics collaboration studied as a tool for the production
}
} } of mirages of the reality. In the domain of computer vision, this
}
} } collaboration may take the form of model-based approaches with
}
} } feedback, as models are essential to process, analyze, understand and
}
} } generate complex still and animated images in a stable manner. Such
}
} } models must be comprehensive and their parameters updated according to
}
} } the difference between the expectation (real image) and the
}
} } synthesized results. In the computer graphics domain, a special
}
} } emphasis will be set upon techniques using real images as input for
}
} } realistic rendering, visualization and special effects.
} }
} } MIRAGE 2003 is going to be an international conference where authors
}
} } are encouraged to submit papers on theoretical, computational,
}
} } experimental and industrial aspects of model-based image analysis and
}
} } synthesis. MIRAGE'2003 will be held in the new conference room of the
}
} } INRIA Rocquencourt research centre on March 10-12, 2003.
} }
} } Topics include (but are not limited to)
} }
} } * Model-based imaging and analysis
} } * Model-based vision approaches
} } * Model-based indexing and database retrieval
} } * Model-based object tracking in image sequences
} } * Image-based rendering and inverse rendering techniques
} } * New trends in model-based (and intrinsic) video compression
} techniques
} } * Virtual and augmented reality
} } * Generation of special effects
} } * Model-based evaluation of computer vision techniques, Image
} quality
} } * Applications:
} } o Human-computer interaction, haptics
} } o Virtual prototyping
} } o Post-production, computer animation
} } o Multimedia applications (including face and gesture
} recognition),
} } multimedia databases
} } o Military applications
} } o Medical and biomedical applications
} } o Applications of fractals and multifractals
} }
} } Venue
} }
} } The Symposium will take place within the INRIA Rocquencourt research
}
} } centre which can be easily reached by car, by train or by bus, either
}
} } by taking public transportations or the free INRIA facilities. The
}
} } different routes are fully detailed on the INRIA special webpage.
} }
} } Paper submission and review process
} }
} } Prospective authors should prepare a full paper and submit it
}
} } electronically. The paper should consist of 5-10 pages in A4 format
}
} } with 11 pt font and should conform to the style guidelines outlined on
}
} } the MIRAGE 2003 website. LaTeX style sheets, MSWord templates and more
}
} } detailed information on the submission process can be found on the
}
} } MIRAGE 2003 website.
} }
} } All submissions will be reviewed by at least 2 members of the Program
}
} } Committee; additional reviewers will be consulted if necessary. The
}
} } papers should provide sufficient background information and should
}
} } clearly indicate the original contribution. They should state and
}
} } discuss the main results and provide adequate references.
} }
} } Confererence proceedings
} }
} } Accepted papers will be published in the CD-Rom conference proceedings
}
} } in pdf-format. Authors will be encouraged to include additional
}
} } multimedia content like sound files, demo's and video sequences,
}
} } subject to space availability. Authors wishing to take advantage of
}
} } this possibility should contact the Steering Committee for additional
}
} } instructions.
} }
} } Important deadlines
} }
} } November 12, 2002 Full paper submission
} } December 20, 2002 Notification of acceptance
} } February 10, 2003 Camera-ready papers due
} } January 6, 2003 Early registration deadline
} } February 10, 2003 Late registration deadline
} } 10-12 March 2003 MIRAGE 2003
} }
} } Registration
} }
} } Registration will be available online at the beginning of
}
} } 2003. However, due to security policy, the number of seats is strictly
}
} } limited: thus, early registration is strongly recommended.
} }
} } Conference Chair
} }
} } Jacques Blanc-Talon, CTA, Arcueil, France
} } Andre Gagalowicz, INRIA, Rocquencourt, France
} }
} } Steering Committee
} }
} } Jacques Blanc-Talon, CTA, Arcueil, France
} } Andre Gagalowicz, INRIA Rocquencourt, France
} } Philippe Gérard, INRIA Rocquencourt, France
} } Wilfried Philips, Ghent University, Gent, Belgium
} } Dominique Potherat INRIA Rocquencourt, France
} }
} } Honorary Chair
} }
} } Hojjat Adeli, Ohio State University, Colombus, USA
} }
} } Program committee
} }
} } Ruzena Bajcsy, UC Berkeley, USA
} } Philippe Bolon, ESIA, Annecy, France
} } Mike Brooks, University of Adelaide, Australia
} } Knut Conradsen, IMM, Denmark Technical University, Denmark
} } Kostas Daniilidis, U-Penn, USA
} } Charles R. Dyer, University of Wisconsin-Madison, USA
} } Andrew Glassner, Coyote Wind, Seattle, USA
} } Cedric Guiard, Duran Duboi, France
} } Adrian Hilton, University of Surrey, UK
} } David Hogg, University of Leeds, UK
} } Xiaoyi Jiang, Technical University of Berlin, Germany
} } Denis Laurendeau, Laval University, Quebec City, Canada
} } Franz Leberl, Graz University of Technology, Austria
} } Ales Leonardis, Ljubljana University, Slovenia
} } Vittorio Murino, Verone University, Italy
} } Jean-Claude Paul, LORIA, France
} } Hichem Sahli, Vrije Universiteit Brussel, Belgium
} } Mubarak Shah, University of Central Florida, USA
} } Gilles Simon, LORIA, Vandoeuvre-les-Nancy, France
} } Franck Solina, Ljubljana University, Slovenia
} } Seah Hock Soon, NTU, Singapore
} } Geoff West, Curtin University, Australia
} } Geoff Wyvill, University of Otago, New Zealand
} } Luc Van Gool, ESAT, Leuven, Belgium (ETH, Zurich, Switzerland)
} }
} }
} --------------------------------------------------------------------------
--
}
}
}
}
} best regards!
}
} André Gagalowicz
}
}



From daemon Tue Sep 3 04:25:06 2002



From: dzemik-at-eko.lublin.pl
Date: Tue, 3 Sep 2002 11:16:33 +0100
Subject: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all !
Maybe somebody knows how to analyse samples of clinoptilolite containing strontium on EDX detector. Strontium and Silicon have both peak in one line. Please help me. This is my doc work.




From daemon Tue Sep 3 10:22:36 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 3 Sep 2002 09:07:26 -0600
Subject: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

I suppose you need the small periodicity to achieve larger "bragg angles" so
you can show the diffraction pattern. If you make your pattern bigger (fewer
dots per inch), your diffraction angles are reduced, but could you perhaps
make up for that by using a longer "camera length", i.e., project the
results on a screen further away, or use some optics? Perhaps you could use
an old slide projector in some inventive fashion.

By the way, what resolution is an 8000 line film recorder? 24 mm / 8000
lines = 3 microns per line or about 8000 dpi?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Monday, September 02, 2002 4:01 PM
To: Microscopy-at-sparc5.microscopy.com


A faculty member needs to produce some extremely high resolution 24 x
35 mm negatives or masks to demonstrate the diffraction phenomenon
and FFT. He has generated around 24 PostScript files that consist of
a "unit cell" (such as an Escher figure) that is repeated numerous
times to mimic a crystalline lattice. The goal is to project a laser
through the negative masks and generate diffraction patterns as part
of the demonstration.

We have attempted to generate hi res 35 mm negs by means of a
LaserGraphics 8,000 line film recorder but the resolution is
inadequate. He needs the equivalent of 16,000 x 24,000 pixels in the
24 x 35 mm area. This is about 17,000 ppi!

Any suggestions? Any other practical ways to produce these masks?

I had considered micro-lithography but do not have access to the technology.

Many thanks,

John B.




##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Sep 3 11:13:08 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Tue, 03 Sep 2002 12:05:28 -0400
Subject: Re: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

A couple of details in your post raised some flags, though I'm not sure of the exact numbers below:

} He needs the equivalent of 16,000 x 24,000 pixels in the 24 x 35 mm area

- Though it's still a topic of debate, I believe the estimate for "pixel equivalent" of a fine-grained 35mm film shot with a high quality lens is around 20 million total pixels (~4000 dpi), which you've already exceeded by quite a bit with the 8,000 line LFR. Even if my memory on this issue is off by millions of pixels, I'm quite sure there is no way 35mm film could support 16,000 x 24,000 pixels.

As far as I can tell, you could get close by upgrading your Lasergraphics film recorder to their 16,000 line "Mark VI" model (16384 x 13448 addressable pixels), and use the 4" x 5" (~ 16k x 20k pixel equivalent film) camera back. You may want to see if some other manufacturer has a film recorder with more than 16,000 lines if you truly need 16,000 x 24,000 pixels.
We have the same 8,000 line LFR in our lab, so I can't give any insight on what the upgrade might cost.

I have no knowledge of or experience with producing masks, so I don't know if 4" x 5" is a viable option.

Kevin Frischmann
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: (212) 313-7975
Fax: (212) 496-3480
email: kfrisch-at-amnh.org
------------------------------------------------------------------------


At 05:00 PM 9/2/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Sep 3 13:02:01 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Tue, 3 Sep 2002 14:31:06 -0400
Subject: Re: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John;

As a follow on, at 17,000 DPI, are you approaching (or passing) the
resolution of the 35mm film?

Does anybody know the size of the Silver Halide Molecules on the film?

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Tuesday, September 03, 2002 2:59 AM
To: John J. Bozzola
Cc: microscopy-at-sparc5.microscopy.com


Aperio's scanner will do this easily - see http://www.aperio.com

-----Original Message-----
} From: Kevin Frischmann [mailto:kfrisch-at-amnh.org]
Sent: Tuesday, September 03, 2002 12:05 PM
To: Microscopy-at-sparc5.microscopy.com


John,

A couple of details in your post raised some flags, though I'm not sure of
the exact numbers below:

} He needs the equivalent of 16,000 x 24,000 pixels in the 24 x 35 mm area

- Though it's still a topic of debate, I believe the estimate for "pixel
equivalent" of a fine-grained 35mm film shot with a high quality lens is
around 20 million total pixels (~4000 dpi), which you've already exceeded by
quite a bit with the 8,000 line LFR. Even if my memory on this issue is off
by millions of pixels, I'm quite sure there is no way 35mm film could
support 16,000 x 24,000 pixels.

As far as I can tell, you could get close by upgrading your Lasergraphics
film recorder to their 16,000 line "Mark VI" model (16384 x 13448
addressable pixels), and use the 4" x 5" (~ 16k x 20k pixel equivalent film)
camera back. You may want to see if some other manufacturer has a film
recorder with more than 16,000 lines if you truly need 16,000 x 24,000
pixels.
We have the same 8,000 line LFR in our lab, so I can't give any insight on
what the upgrade might cost.

I have no knowledge of or experience with producing masks, so I don't know
if 4" x 5" is a viable option.

Kevin Frischmann
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: (212) 313-7975
Fax: (212) 496-3480
email: kfrisch-at-amnh.org
------------------------------------------------------------------------


At 05:00 PM 9/2/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Tue Sep 3 13:41:32 2002



From: Lois Anderson :      landers-at-jhmi.edu
Date: Tue, 03 Sep 2002 14:35:24 -0400
Subject: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone ever trained an inexperienced EM tech and if so do you have
any suggested tactics?


From daemon Tue Sep 3 15:18:36 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 3 Sep 2002 15:09:49 -0500
Subject: Sorvall ultramicrotome available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sorvall MT-2B Ultramicrotome for sale. Includes manual and accessories. If interested, contact me via e-mail.

Martin L. Katz, PH.D.
University of Missouri
Ophthalmology, Genetics, Pathobiology & Molecular Biology
Mason Eye Institute
One Hospital Drive
Columbia, MO 65212
(573) 882-8480
FAX (573) 884-4100
katzm-at-health.missouri.edu

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Tue Sep 3 15:35:28 2002



From: Giles, Bill :      William.Giles-at-timet.com
Date: Tue, 3 Sep 2002 14:28:44 -0600
Subject: RE: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try looking at 14.164 Kev, should be a K line down there.

William Giles
Senior Electron Microscopist
Metallography Lab Coordinator
P.O. Box 2128
Henderson, Nevada 89009
(702) 566-4436
(702) 564-9038 (Fax)
Bill.Giles-at-timet.com

RE:
Hello all !
Maybe somebody knows how to analyse samples of clinoptilolite containing
strontium on EDX detector. Strontium and Silicon have both peak in one line.
Please help me. This is my doc work.



*


From daemon Tue Sep 3 16:53:57 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 03 Sep 2002 14:48:04 -0700
Subject: RE: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I missed on this one too. The fellow is looking for making a
slide, not scanning one. I think the highest resolution is probably
via a microcircuit stepper mask (5"x5" chrome on quartz).
The image size can be just about anything up to the size of
the plate. Price is big too.

gary

At 11:31 AM 9/3/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Sep 3 16:56:15 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 3 Sep 2002 17:56:10 -0400
Subject: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John;

Photolithography, as is used in semiconductors, would certainly give you the
spatial resolution you desire but is an expensive proposition for a
demonstraion.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Monday, September 02, 2002 6:01 PM
To: Microscopy-at-sparc5.microscopy.com


A faculty member needs to produce some extremely high resolution 24 x
35 mm negatives or masks to demonstrate the diffraction phenomenon
and FFT. He has generated around 24 PostScript files that consist of
a "unit cell" (such as an Escher figure) that is repeated numerous
times to mimic a crystalline lattice. The goal is to project a laser
through the negative masks and generate diffraction patterns as part
of the demonstration.

We have attempted to generate hi res 35 mm negs by means of a
LaserGraphics 8,000 line film recorder but the resolution is
inadequate. He needs the equivalent of 16,000 x 24,000 pixels in the
24 x 35 mm area. This is about 17,000 ppi!

Any suggestions? Any other practical ways to produce these masks?

I had considered micro-lithography but do not have access to the technology.

Many thanks,

John B.




##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Sep 3 17:05:21 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 3 Sep 2002 15:42:21 -0700
Subject: Re: LF shielded computer monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

there is no simple answer to your question, as the speed of the film has a
big influence on the resolution (faster films use larger grains). Also
development will change the number of pixels. I did a quick search on the
internet and I found 2 sites with specific answers. I have included excerpts
below, there is more information on those sites.

It seems, the answer is somewhere between 10 Million and 20 Million pixels,
but that is of course only a qualitative answer. A full answer would have to
take into account the modulation transfer function, dynamic range, etc. The
second link below goes into that a bit. For example, the author says that a
certain film can resolve 3500 line pairs (7000 pixels) over the width of the
film (35mm), but that only means, that at that resolution one can
distinguish a white line from a black line with some accuracy. In digital
parlance that would probably mean a dynamic range of 1 bit (black or white).
If you want to have a better dynamic range (to distinguish for example black
from dark gray), you would have to look at larger areas, thus reducing the
resolution.

mike



Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================


http://pic.templetons.com/brad/photo/pixels.html
How many pixels are there in a frame of 35mm film?

(an FAQ on digital photography)

This is a somewhat controversial question, and there are many possible
answers. Film is an analog medium, so it doesn't have "pixels" per se,
though film scanners have pixels and a specific resolution.

Today the one thing most people agree on is that it's a lot more than any
current consumer digital camera. The debate is about how much resolution the
digitals will have to reach to start matching the film.

The very short answer is that there are around 20 million "quality" pixels
in a top-quality 35mm shot. That's a shot with a tripod, mirror-up, with a
top-rate lens and the finest-grained film, in decent light. 12 million are
more typical for "good" shots. There may be as few as 4 million "quality"
pixels in a handheld shot with a point-and-shoot camera or camera with a
poor lens. And of course if focus is poor, or light is poor, or the camera
was not held steady, the number will drop down below the 1-2 million pixels
of the modern consumer digicam. Of course, one can have a bad shot with a
digital camera too, not using all its resolving ability. However, few pick
their gear with the plan of shooting badly.
..


http://www.bytesmiths.com/Services/scanning.html
The dimensions of a digital image are often confused with its resolution,
but it is actually a count of pixels (width by height) that is independent
of any unit of measure. A highest-quality, 35mm film scan will have
dimensions of about 3600x2400 pixels.
..





-----Original Message-----
} From: John W. Raffensperger, Jr. [mailto:johnr-at-helwigcp.com]
Sent: Tuesday, September 03, 2002 11:51 AM
To: microscopy-at-sparc5.microscopy.com


John;

As a follow on, at 17,000 DPI, are you approaching (or passing) the
resolution of the 35mm film?

Does anybody know the size of the Silver Halide Molecules on the film?

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Tuesday, September 03, 2002 2:59 AM
To: John J. Bozzola
Cc: microscopy-at-sparc5.microscopy.com


http://www.themonitoroutlet.com
has every monitor known to man.

John Mardinly
Intel

-----Original Message-----
} From: Ping Li [mailto:pli-at-dal.ca]
Sent: Friday, August 30, 2002 4:56 AM
To: Microscopy-at-sparc5.microscopy.com


Could some of you please provide me some information regarding where to
purchase a 21" or larger low frequency shielded computer monitor? I would
like to buy one but having trouble to find a supplier. The monitor will be
used to replace the smaller one on our Tecnai TEM. Your help is greatly
appreciated.

Thank you,
Ping Li

--
Ping Li, Ph.D.
Director, Scientific Imaging Suite
Department of Biology
Dalhousie University
Halifax, NS B3H 4J1
Canada

Tel: 902-494-3309
Fax: 902-494-3736
E-mail: Ping.Li-at-Dal.Ca




From daemon Tue Sep 3 20:36:05 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 4 Sep 2002 13:26:19 +1200
Subject: Re: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} Hello all !
} Maybe somebody knows how to analyse samples of clinoptilolite
} containing strontium on EDX detector. Strontium and Silicon have both
} peak in one line. Please help me. This is my doc work.
}
}
}

WDS would be better, but if you just can't get to it, maybe you could
bang the accelerating voltage up to 25 or even 30 kV and analyse
on the Sr Ka line at about 15 kV. I haven't done it for Sr, but I did
use 25 kV for Mo Ka to avoid SKa, worked OK.

good luck

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Sep 3 23:38:50 2002



From: Regina Himmelspach :      Himmelspach-at-rsbs.anu.edu.au
Date: Wed, 04 Sep 2002 14:27:17 +1000
Subject: TEM; need help on LR gold embedding in the Leica EM AFS freeze

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi all,

I would appreciate some advice on low temperature embedding in the Leica EM
AFS freeze substitution unit using LR gold. Sorry, since I am a beginner
(concerning TEM) some of my questions might sound a little odd.

My aim is to do immunogold labelling, mainly on cell walls, with sections
from Arabidopsis roots. The plan is to high pressure freeze Arabidopsis
roots, freeze substitute them, and embed them in LR gold at low temperature
(-25 degrees).

At the moment I am running a few tests before I start the real experiment.
And I am having trouble with the LR gold embedding.

This is what I tried so far:
I followed the protocol for "FS and low temperature embedding in
LEICA-capsules" from the AFS handbook (5.2, p.35). I was using LR gold +
0.5% Benzil (w/v) as embedding medium. I started polymerisation at -25
degrees with the UV lamp (Leica EM UV). After 24h the resin was not yet
solidified, only the bottom half of the capsules looked like it was solid.

My questions:
Does anybody have experience with low temperature embedding using LR gold
in the Leica EM AFS? Any ideas how long UV-polymerisation might take? (24h
was the information I got from the supplier for the LR gold). Or is there an
easier way of getting my specimens embedded, preferably in the Leica EM
AFS?

Thanks a lot for your help,

Regina



-------------------------------------------------------------------
Dr. Regina Himmelspach
Plant Cell Biology Group
Research School of Biological Sciences
The Australian National University
GPO Box 475
Canberra ACT 2601
Australia

ph: (+02) 6125-5561
fax: (+02) 6125-4331
himmelspach-at-rsbs.anu.edu.au




From daemon Tue Sep 3 23:42:28 2002



From: Allen Sampson :      ars-at-sem.com
Date: Tue, 3 Sep 2002 23:39:43 -0700
Subject: RE: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Interesting question, particularly now when EMs are found in such a wide
range of applications. I'll share what seems to work for me, although I
make no claim that this is the way training should be done.

I've trained quite a number of operators. I want them to first know those
things that can cause damage (i.e. - don't touch that TEM phosphor screen).
The instruments are frightening at first, and having some limits will
actually be comforting to them - they can focus on those things and relax
with the rest. Don't overload them with don't do's - just cover the things
that may cause expensive or time consuming damage.

My own desire is always to have the operator understand the physics behind
the instrument, and I always explain things in those terms. Knowing that
increasing the condenser current in an EM decreases the spot size gives you
little. Knowing that it reduces the chromatic spread or aberration of the
beam, and how, gives a broader understanding of the mechanism and the
result. While the distinction may not be terribly helpful in normal use it
gives them something that may help in the unusual circumstances.

That's what you really have to plan for in training, the unusual
circumstances. I've seen a number of customers in recent years who have
absolutely no knowledge of the instruments. 'Checklist operators' I call
them, and that's literally how they work. The person who bought the
instrument is long gone from the company, but he left a checklist for the
operation of the instrument that has been faithfully followed since. It's
a minor annoyance for me since I get service calls when someone
accidentally touches the wrong knob or they get a different type of sample
where the standard setup won't work. That stuff is minor, though, and
truly the extreme.

These are some really great tools, kind of like a hammer. When you need to
drive a nail, nothing works better. When you need to look at something
with a magnification greater than a light microscope, nothing beats EM.
But they are getting more trivialized as more production use is made of
them. These are more than a simple tool - they are the most sophisticated
equipment you'll find in a lab. Their proper and useful use requires
operators who know their limitations as well as their operation. One of
the things I always want to ensure is that the operators, if not the
management, understand what to really expect regarding the application and
interpretation of EMs.

All of this is in a lecture setting - I don't expect them to consciously
remember what I've told them over the hour or two it takes. Rather, I hope
that when they need it, they'll connect the dots with something I said and
figure out for themselves what's needed.

Then it's on to hands on training. This is generally very short - I can
teach someone to take a usable picture in five minutes. A little more time
looking over their shoulder while they look around. But then it's time to
get out.

The next two steps are the most important, bar none. Give them time to
play and learn for themselves the questions to ask, and follow up.

It takes time to get comfortable operating an instrument like these. It
takes time to play around with different samples and conditions. It takes
time to discover the questions you need help answering. The potential
operators simply have to spend a lot of time playing, not working, with an
EM to become good operators. If they are required to immediately start
working, you may get a good 'checklist operator' for the samples you
regularly run, and that may be enough. But if you want someone who really
understands the instrument, its use and its limitations, you have to
encourage them to explore the envelope.

Follow up - you have to follow up. Don't do it too quick, give them some
time to play. You have to take the initiative to query the potential
operator regarding the questions they have accumulated (suggest they write
them down) and the samples and conditions they have explored. This is your
chance to add details and refine their knowledge of the use and limitations
of the instrument. They're starting to learn the questions to ask - they
need to know that there is someone there to help with these problems. If
you're not there, then they will come to their own conclusions. Sometimes
they might be right, sometimes they might be wrong - in either case, if you
don't follow up you'll be missing out on your best possibility to really
train.

Have fun!


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Tuesday, September 03, 2002 11:35 AM, Lois Anderson
[SMTP:landers-at-jhmi.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Has anyone ever trained an inexperienced EM tech and if so do you have
} any suggested tactics?
}



From daemon Wed Sep 4 06:46:55 2002



From: Frank Thomas :      thomasf-at-gsca.NRCan.gc.ca
Date: Wed, 4 Sep 2002 08:32:37 -0300
Subject: LaB6 cathodes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers -

Does anyone know of any companies out there that rebuild LaB6 cathodes?
Has anyone had good/bad experiences with them?
Vendors, please reply off-list....

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada



From daemon Wed Sep 4 07:32:58 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 4 Sep 2002 08:31:17 -0400
Subject: RE: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The price is ~ $5K [U.S.] per photomask.

Peter

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Tuesday, September 03, 2002 5:48 PM
To: microbill-at-mohawk.net
Cc: MSA listserver


I missed on this one too. The fellow is looking for making a
slide, not scanning one. I think the highest resolution is probably
via a microcircuit stepper mask (5"x5" chrome on quartz).
The image size can be just about anything up to the size of
the plate. Price is big too.

gary

At 11:31 AM 9/3/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Sep 4 07:45:24 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 4 Sep 2002 08:44:07 -0400
Subject: RE: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Bill;

It's true that Si & Sr overlap at ~ 1.8 KeV. The Sr major La1 line is at
1.806 KeV and the Si Ka1 major line is at 1.739 KeV. However, Sr is a much
more complex atom and has a Ka1 major line at 14.164 KeV as Bill Giles
mentioned below. Sr should be easily discernable from Si. Of course you
will have to have an incident electron energy of about 1.5 to 2X of the Sr
Ka1 energy to see it. I assume you can run your survey at 25 to 30 KeV?

Peter Tomic
Anadigics, Inc.



-----Original Message-----
} From: Giles, Bill [mailto:William.Giles-at-timet.com]
Sent: Tuesday, September 03, 2002 4:29 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Try looking at 14.164 Kev, should be a K line down there.

William Giles
Senior Electron Microscopist
Metallography Lab Coordinator
P.O. Box 2128
Henderson, Nevada 89009
(702) 566-4436
(702) 564-9038 (Fax)
Bill.Giles-at-timet.com

RE:
Hello all !
Maybe somebody knows how to analyse samples of clinoptilolite containing
strontium on EDX detector. Strontium and Silicon have both peak in one line.
Please help me. This is my doc work.



*


From daemon Wed Sep 4 07:51:48 2002



From: JHoffpa464-at-aol.com
Date: Wed, 04 Sep 2002 08:44:46 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


are you talking about training from scratch? ie fixation, sectioning etc? if so there is a long row to hoe. the best bet is always start from the somplest to the more complicated, and train in the order they would normaly do things, such as embedding then triming then sections (thicks then thins) staining then finaly to the scope.
always include the theory in with the practical. depending on how motivated the person is and how big an ego they have traing could take anywhere from a week to the person never getting it.
when i learned Em it was in a class room setting with the prof showing us once how to do things then for the rest of the semester we were on our own, lots of students droped the course. the few that stuck it out became very good and advanced techs. this is not i repeat not the recommended method of training someone. i still have thoughts of how to get even with the prof.
john


From daemon Wed Sep 4 08:57:07 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 4 Sep 2002 09:40:58 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Has anyone ever trained an inexperienced EM tech and if so do you have
} any suggested tactics?

***************
I have taught and EM course a number of times and have trained
technicians who had no background. Slow and steady wins the race. I
have found that taking the time to explain WHY things are done, and
WHAT the aims of each procedure are is very helpful. You can just
make people memorize techniques, but without understanding they will
always be automatons and won't be able to deal with anything
unexpected. So, don't just give them a dehydration protocol, explain
why you need to extract the water, etc. Also, take it one step at a
time, don't try to teach the person everything, tissue to grid, in
one fell swoop.

The good ting is that you can teach this person how to do things the
way you like, and they don't have any conflicting ideas.

Good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Sep 4 08:57:07 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Wed, 4 Sep 2002 10:22:25 -0400
Subject: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Allen touched on a significant point. I haven't had the pleasure of
working with an EM, but the concept applies to other technology as well.

As the "front ends" of technological devices get "better" and easier to
use, it becomes easier and easier to "get results". Just about anyone
can get some type of reading/image/measurement from a piece of modern
equipment.

Getting a result, and getting a MEANINGFUL result are two very different
things. Without a background understanding of the fundamental concepts
involved in how a particular device/technology works, the operator is at
risk of getting a result that may appear meaningful, but is beyond the
capabilities of the equipment (and/or sample preparation techniques).

An example I can directly relate to is on a 3-D Coordinate Measuring
Machine. In the "old days", the interfaces were crude, and you needed
to have a pretty thorough understanding of 3-D geometry just to get a
reading. Now, with the graphical interfaces, just about anyone can walk
up, use the joystick and touch the part in a few places, and get a
reading. But without the fundamental understanding, they may not have
set a meaningful reference plane and axis. As a result, they did indeed
get a result, but a meaningless one.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Allen Sampson [mailto:ars-at-sem.com]
Sent: Wednesday, September 04, 2002 1:40 AM
To: 'Lois Anderson'; microscopy-at-sparc5.microscopy.com


Dear Wil,

We are the authorized Polaron dealer in the U.S. Please contact us at
ebs-at-ebsciences.com or by phone on (800) 992-9037 and we will be happy to
help you with any parts, service or new instrument requirements you have.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: "curari-at-asu.edu"-at-sparc5.microscopy.com
[mailto:"curari-at-asu.edu"-at-sparc5.microscopy.com]
Sent: Saturday, August 31, 2002 8:36 PM
To: Microscopy


Dear Listers

I am looking for a replacement part for our poloron sputter/coater.
It
is a model 0E5000, manufacturing date unknown. The part that is broken is a
hollow glass cylinder that has a rubber seal at either end and acts as the
sputtering chamber. Any recommendations on a supplier or glass manufacturer
is
welcomed or other ideas! Thank you in advance.

Wil Kunkel
curari-at-asu.edu
student of chemistry
This email was sent with 100.00% recycled electrons.




From daemon Wed Sep 4 09:55:34 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Wed, 4 Sep 2002 10:47:56 -0400
Subject: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Wil,

We are the authorized Polaron dealer in the U.S. Please contact us at
ebs-at-ebsciences.com or by phone on (800) 992-9037 and we will be happy to
help you with any parts, service or new instrument requirements you have.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: "curari-at-asu.edu"-at-sparc5.microscopy.com
[mailto:"curari-at-asu.edu"-at-sparc5.microscopy.com]
Sent: Saturday, August 31, 2002 8:36 PM
To: Microscopy


Dear Listers

I am looking for a replacement part for our poloron sputter/coater.
It
is a model 0E5000, manufacturing date unknown. The part that is broken is a
hollow glass cylinder that has a rubber seal at either end and acts as the
sputtering chamber. Any recommendations on a supplier or glass manufacturer
is
welcomed or other ideas! Thank you in advance.

Wil Kunkel
curari-at-asu.edu
student of chemistry
This email was sent with 100.00% recycled electrons.




From daemon Wed Sep 4 11:27:50 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 4 Sep 2002 12:19:09 -0400
Subject: RE: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lois,

We train 8-16 students how to use the SEM and TEM a year here. In
general 99% of them have no clue where to begin. I do follow pretty
much a line similar to what Allan Sampson talked about. BUT I don't go
deep into the physics of the system until they get out of the intro
class and into the advanced class. We have a 3 check out system. First
one is 2 hours with 2 students and me. Sample loading - what you can do
to screw it up - "review the checklist" - and generally go over the
basics - sample moving, mag, focus, collecting an image. The second
checkout is 2 hours with 1 student. I sit down and spend 15 minutes
going over everything with them and then leave them there with
instructions to collect 3-4 images of what ever they want (sample
already in). I check on them through the 2 hours - answer questions,
offer pointers, generally lightly supervise them. The final session is
basically instruction free. A test if you will. One so that they can
demonstrate to me that they understand enough about what they are doing
so as not to break anything and then they get keys to the rooms.
Instruction is carried out in the classes on specific areas with
demonstrations and lab write-ups. But even for student researchers that
are not enrolled in the class, they receive very similar instruction,
but tailored more to their individual project. The emphasis is always -
ask questions! Make sure they realize that if they are stuck then need
to ask questions rather than waste an hour or more of beam time trying
to figure something out themselves.

The standard checklist protocol is critical, but it can't be a
substitute for hands on instructions. Scare them but not enough that
they are afraid to touch stuff, reassure them so they have confidence
but not enough that they are more likely to break something. Emphasize
that if they DO do something resulting in a failure or a problem that
they document exactly what they did leading up to the problem and that
they won't get in trouble and that it is essential that what happened
leading up to the problem be fully disclosed so that the problem can be
fixed as fast as possible.

Hope this helps - it works for us here.

Geoff Williams
Microscopy Facility Supervisor

Checkout the new Biology Department Microscopy Facility web page.
Version 1 is now On-Line:
www.cst.cmich.edu/users/willi1gl/BDMF/BDMF.htm

} -----Original Message-----
} From: Lois Anderson [mailto:landers-at-jhmi.edu]
} Sent: Tuesday, September 03, 2002 2:35 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: trainee
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} Has anyone ever trained an inexperienced EM tech and if so do you have
} any suggested tactics?



From daemon Wed Sep 4 12:51:47 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 4 Sep 2002 12:43:49 -0500
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In training or help to train dozens of people over the years, I have run across one phenomenon that has always puzzled me. I first noticed this when showing people how to do specimen exchanges in TEM's in which a premature turning of the specimen arm before sufficient pumping on the airlock causes the high-voltage to shut down. Sometimes it would take around 20 minutes to get the scope back on line, and there can actually be introduction of a bit of oil into the column.

Needless to say, I wanted to warn trainees about this, so I first started telling them something like "Insert the specimen arm, BUT DO NOT TURN IT UNTIL....." Almost invariably, this resulted in exactly what I wanted to avoid, usually the first or second time I wasn't there to watch. Finally, I switched to saying "Insert the specimen arm and push it into place with the flat of your hand, then after the light goes out, turn it clockwise". That seems to work.

The point is, I guess, that in training people about things that can potentially damage equipment, I have found it best NOT to say "Don't ever do this!" It works much better (for me) to say "Do it like this." and demonstrate a way that minimizes the chance of an accident. Later on, after the habit has been established, I can explain the reasons without causing bad things to happen.

Has anyone else experienced this? Is this Edgar Allen Poe's "Imp of the Perverse" or just, gulp, me?

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Wed Sep 4 12:51:47 2002



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Wed, 4 Sep 2002 13:49:20 -0400
Subject: M&M 2002 Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is it too early to inquire as to the status of mailings of the proceedings?
If not, could someone direct me to the appropriate individual or committee
responsible for the mailings so that I could determine when I could expect
mine to arrive. I seem to remember that last year there were many postings
to the list on the subject of late deliveries. I haven't seen any this
year, so maybe I'm the exception. Please disregard this note if my copy is
already in my mailbox.





From daemon Wed Sep 4 14:28:11 2002



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Wed, 4 Sep 2002 15:22:10 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Randy,
This is called Neuro-Linguistic Programming (NLP) by some.
http://www.nlptraining.com/index.asp

Disclaimer: The above link is for your information only.



} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} X-OriginalArrivalTime: 04 Sep 2002 17:43:49.0465 (UTC)
}
}
} In training or help to train dozens of people over the years, I have run
} across one phenomenon that has always puzzled me. I first noticed this
} when showing people how to do specimen exchanges in TEM's in which a
} premature turning of the specimen arm
} before sufficient pumping on the airlock causes the high-voltage to shut
} down. Sometimes it would take around 20 minutes to get the scope back on
} line, and there can actually be introduction of a bit of oil into the
} column.
}
} Needless to say, I wanted to warn trainees about this, so I first started
} telling them something like "Insert the specimen arm, BUT DO NOT TURN IT
} UNTIL....." Almost invariably, this resulted in exactly what I wanted to
} avoid, usually the first or second
} time I wasn't there to watch. Finally, I switched to saying "Insert the
} specimen arm and push it into place with the flat of your hand, then after
} the light goes out, turn it clockwise". That seems to work.
}
} The point is, I guess, that in training people about things that can
} potentially damage equipment, I have found it best NOT to say "Don't ever
} do this!" It works much better (for me) to say "Do it like this." and
} demonstrate a way that minimizes the
} chance of an accident. Later on, after the habit has been established, I
} can explain the reasons without causing bad things to happen.
}
} Has anyone else experienced this? Is this Edgar Allen Poe's "Imp of the
} Perverse" or just, gulp, me?
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
354 Mansfield Road
Beach Hall, Room 129
Storrs, CT 06269-2242

860 486-2914 voice
860 486-6369 fax
james.romanow-at-uconn.edu




From daemon Wed Sep 4 15:42:30 2002



From: Kim Rensing :      krensing-at-interchange.ubc.ca
Date: Wed, 04 Sep 2002 13:33:41 -0700
Subject: Re: TEM; need help on LR gold embedding in the Leica EM AFS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hi all,
}
} I would appreciate some advice on low temperature embedding in the Leica EM
} AFS freeze substitution unit using LR gold. Sorry, since I am a beginner
} (concerning TEM) some of my questions might sound a little odd.
}
} My aim is to do immunogold labelling, mainly on cell walls, with sections
} from Arabidopsis roots. The plan is to high pressure freeze Arabidopsis
} roots, freeze substitute them, and embed them in LR gold at low temperature
} (-25 degrees).
}
} At the moment I am running a few tests before I start the real experiment.
} And I am having trouble with the LR gold embedding.
}
} This is what I tried so far:
} I followed the protocol for "FS and low temperature embedding in
} LEICA-capsules" from the AFS handbook (5.2, p.35). I was using LR gold +
} 0.5% Benzil (w/v) as embedding medium. I started polymerisation at -25
} degrees with the UV lamp (Leica EM UV). After 24h the resin was not yet
} solidified, only the bottom half of the capsules looked like it was solid.
}
} My questions:
} Does anybody have experience with low temperature embedding using LR gold
} in the Leica EM AFS? Any ideas how long UV-polymerisation might take? (24h
} was the information I got from the supplier for the LR gold). Or is there an
} easier way of getting my specimens embedded, preferably in the Leica EM
} AFS?
}
} Thanks a lot for your help,
}
} Regina

Regina,

LR Gold won't polymerize completely if there is oxygen present. The AFS is
supposed to exclude O2 by evaporating some of the LN2, but if there is a lot
of air movement around the machine (i.e., lots of people walking by, or in
front of an air vent) then it doesn't work so well. Make sure the inside
glass is in place during UV exposure.

If the bottom of the capsule is hard, you can pour off the top liquid, cut
off the goopy layer and use the hard bottom layer containing your specimen.

You should also make sure you are not introducing water (frost or
condensation) during processing. That will also prevent polymerization. You
may also retain some from the specimen if substitution is not complete.

Ask if you have more questions.

Good luck,

Kim

-------
Kim Rensing Ph.D.
Dept. of Botany, UBC
6270 University Blvd
Vancouver, BC, Canada
V6T 1Z4

phone: 604-822-5223
fax: 604-822-6089



From daemon Wed Sep 4 17:55:17 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 4 Sep 2002 18:43:06 -0400
Subject: RE: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My procedure is to get out a box of razor blades and a bigger box of
Band-Aids. Then I demonstrate how to trim a block - bloodlessly. Then I do
it again, and again. The third trapezoid always has a base that is between
.5 and .75 mm. Then, I explain that I cut myself twice in the first week
and never again.

By the time I have finished and watched the first 10 minutes, I know whether
there is going to be a need for transfusions or not. If not, I walk away
for 20 minutes before I come back and inspect their work. I always make a
point to repair those attempts that are not 'up top snuff' and gush over
those that are. Then I make the gushers help the hindered until everyone is
on the same page.

One lab that way, usually the second, and sectioning always goes 50% better
than if I neglected to bring the Band-Aids. The first lab is always spent
cleaning the microscope.

Train 'em up good.

I had a sign on the outer back of the revolving darkroom door, which was
exposed whenever anyone entered and revolved it to all of those who looked
when they heard its noise. "Please don't enter the darkroom with the lights
on." And, I never fixed it or moved it. Somehow, after the first week, no
one ever left those light on either!

Hope you are well. I'm getting new scopes within next two months. New TEM
(FEI Technai 12T) and new ESEM (FEI Quanta 400) w/EDS. These days such
riches remind me of the poor man who won a Lincoln but had no money for gas.
There I'll sit for weeks after my Technai 12T is up and running, daintily
cutting grids from mesh with dull iris scissors. Then I'll have to go to
three Mile Island for uranium salts and to West Philadelphia for lead
tailings under old exterior window sills. And while I'm doing those
collections, I'll take a few hits by random gammas and then get mugged.
Microscopy is wonderful. Well, all won't be sad. I can analyze the skin on
my finger tips for contamination of Pb and U for no cost and under only
slight vacuum in my Quanta, and they won't dry out and I won't get
beam-burned.

The pre-installation costs are mounting to around $25,000 for three 208V (1
x 60 and 2 x 20A) supplies, removal of 30 ft. of base cabinetry, capping
plumbing of three sinks, running a dead 60A line for 15 ft, removing 1
fluorescent and 2 incandescent fixtures, and installing 50' of sound
proofing to reduce acoustic noise (of my screams!). We thankfully have
avoided the cost of jacking up our end of the building to determine the
cause of any vibrations, because there were none.

Cheers,

Fred

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Leona Cohen-Gould
} Sent: Wednesday, September 4, 2002 9:40 AM
} To: Lois Anderson; microscopy-at-sparc5.microscopy.com
} Subject: Re: trainee
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} }
} } Has anyone ever trained an inexperienced EM tech and if so do you have
} } any suggested tactics?
}
} ***************
} I have taught and EM course a number of times and have trained
} technicians who had no background. Slow and steady wins the race. I
} have found that taking the time to explain WHY things are done, and
} WHAT the aims of each procedure are is very helpful. You can just
} make people memorize techniques, but without understanding they will
} always be automatons and won't be able to deal with anything
} unexpected. So, don't just give them a dehydration protocol, explain
} why you need to extract the water, etc. Also, take it one step at a
} time, don't try to teach the person everything, tissue to grid, in
} one fell swoop.
}
} The good ting is that you can teach this person how to do things the
} way you like, and they don't have any conflicting ideas.
}
} Good luck,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}


From daemon Wed Sep 4 19:26:01 2002



From: Seth Ness :      seth.ness-at-mssm.edu
Date: Wed, 04 Sep 2002 20:04:58 -0400
Subject: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi,

I am isolating a rare monocyte population by FACS and trying to
look at it by TEM. on the first go around i isolated 8500 cells and
the pathologists were unable to get images of any cells becuase
the pellet was too small to see.

Are there any techniques to allow the imaging of very small
numbers of cells?


thanks,
---
Seth L. Ness M.D., Ph.D
Fellow in Human Genetics
Department of Human Genetics
Mount Sinai School of Medicine

Phone:212-241-6947
Fax:212-860-3316
sln8-at-columbia.edu

Ness Gadol Haya Sham



From daemon Wed Sep 4 19:59:43 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 04 Sep 2002 17:54:42 -0700
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:35 AM 9/3/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Flog them until they get with the program. However, this does
not adhere to ISO-9000 principles. But, who knows, perhaps
this could someday be included?

gg



From daemon Wed Sep 4 20:07:44 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 4 Sep 2002 21:06:35 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim;

You are not in the ether alone. I believe what happens is that sometimes
when an operator, or anyone for that matter, reads instructions, they
anticipate the next step in the procedure and sometimes they are wrong. We
have all done this in simple things like assembling toys or anything with
mundane instructions that seem intuitive when they aren't necessarily and
have several outcomes or degrees of freedom. I have been scolded on more
occasions than I want to recall by my wife for "NOT READING THE INSTRUCTIONS
ALL THE WAY FIRST!" Me being an engineer has yet to impress her in these
matters. She mentioned nothing about neuro-lingusitic programming whilst
yelling this at me. Needless to say I had to take the thing apart again.

Peter



-----Original Message-----
} From: Jim Romanow [mailto:bsgphy3-at-uconnvm.uconn.edu]
Sent: Wednesday, September 04, 2002 3:22 PM
To: microscopy-at-sparc5.microscopy.com




Randy,
This is called Neuro-Linguistic Programming (NLP) by some.
http://www.nlptraining.com/index.asp

Disclaimer: The above link is for your information only.



} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} X-OriginalArrivalTime: 04 Sep 2002 17:43:49.0465 (UTC)
}
}
} In training or help to train dozens of people over the years, I have run
} across one phenomenon that has always puzzled me. I first noticed this
} when showing people how to do specimen exchanges in TEM's in which a
} premature turning of the specimen arm
} before sufficient pumping on the airlock causes the high-voltage to shut
} down. Sometimes it would take around 20 minutes to get the scope back on
} line, and there can actually be introduction of a bit of oil into the
} column.
}
} Needless to say, I wanted to warn trainees about this, so I first started
} telling them something like "Insert the specimen arm, BUT DO NOT TURN IT
} UNTIL....." Almost invariably, this resulted in exactly what I wanted to
} avoid, usually the first or second
} time I wasn't there to watch. Finally, I switched to saying "Insert the
} specimen arm and push it into place with the flat of your hand, then after
} the light goes out, turn it clockwise". That seems to work.
}
} The point is, I guess, that in training people about things that can
} potentially damage equipment, I have found it best NOT to say "Don't ever
} do this!" It works much better (for me) to say "Do it like this." and
} demonstrate a way that minimizes the
} chance of an accident. Later on, after the habit has been established, I
} can explain the reasons without causing bad things to happen.
}
} Has anyone else experienced this? Is this Edgar Allen Poe's "Imp of the
} Perverse" or just, gulp, me?
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
354 Mansfield Road
Beach Hall, Room 129
Storrs, CT 06269-2242

860 486-2914 voice
860 486-6369 fax
james.romanow-at-uconn.edu




From daemon Thu Sep 5 02:17:04 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 5 Sep 2002 08:07:22 +0100
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} (FEI Technai 12T) and new ESEM (FEI Quanta 400) w/EDS. These days
such
} riches remind me of the poor man who won a Lincoln but had no money
for gas.
} There I'll sit for weeks after my Technai 12T is up and running,
daintily
} cutting grids from mesh with dull iris scissors. Then I'll have to
go to
} three Mile Island for uranium salts and to West Philadelphia for
lead
} tailings under old exterior window sills.

Luxury! We're so poor we have to shrink chicken wire in the washing
machine to make grids!
C.

} And while I'm doing those
} collections, I'll take a few hits by random gammas and then get
mugged.
} Microscopy is wonderful. Well, all won't be sad. I can analyze the
skin on
} my finger tips for contamination of Pb and U for no cost and under
only
} slight vacuum in my Quanta, and they won't dry out and I won't get
} beam-burned.
}
} The pre-installation costs are mounting to around $25,000 for three
208V (1
} x 60 and 2 x 20A) supplies, removal of 30 ft. of base cabinetry,
capping
} plumbing of three sinks, running a dead 60A line for 15 ft, removing
1
} fluorescent and 2 incandescent fixtures, and installing 50' of sound
} proofing to reduce acoustic noise (of my screams!). We thankfully
have
} avoided the cost of jacking up our end of the building to determine
the
} cause of any vibrations, because there were none.
}
} Cheers,
}
} Fred
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} c/o Geology/Astronomy
} West Chester University
} South Church Street and Rosedale Ave
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
} } ----------
} } From: Leona Cohen-Gould
} } Sent: Wednesday, September 4, 2002 9:40 AM
} } To: Lois Anderson; microscopy-at-sparc5.microscopy.com
} } Subject: Re: trainee
} }
}
} --------------------------------------------------------------------
----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
---.
} }
} }
} } }
} } } Has anyone ever trained an inexperienced EM tech and if so do you
have
} } } any suggested tactics?
} }
} } ***************
} } I have taught and EM course a number of times and have trained
} } technicians who had no background. Slow and steady wins the race.
I
} } have found that taking the time to explain WHY things are done,
and
} } WHAT the aims of each procedure are is very helpful. You can
just
} } make people memorize techniques, but without understanding they
will
} } always be automatons and won't be able to deal with anything
} } unexpected. So, don't just give them a dehydration protocol,
explain
} } why you need to extract the water, etc. Also, take it one step at
a
} } time, don't try to teach the person everything, tissue to grid,
in
} } one fell swoop.
} }
} } The good ting is that you can teach this person how to do things
the
} } way you like, and they don't have any conflicting ideas.
} }
} } Good luck,
} } Lee
} } --
} } Leona Cohen-Gould, M.S.
} } Sr. Staff Associate
} } Director, Electron Microscopy Core Facility
} } Manager, Optical Microscopy Core Facility
} } Joan & Sanford I. Weill Medical College
} } of Cornell University
} } voice (212)746-6146
} } fax (212)746-8175
} }
} }
}



From daemon Thu Sep 5 02:39:44 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Thu, 05 Sep 2002 09:32:38 +0200
Subject: Long life-time fluorophore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,

First of all,
Sorry if some of you receive this email more than once, I'm sending this to
3 mailinglists at the same time...
I have a question about a fluorophore. I'm looking for a secondary
fluorescent antibody that has a really long life-time. The purpose is to
inject fixed cells (staining before fixation) into a mice so I can measure
the number of fluorescent cells in an organ after 60 days. I was thinking
about carboxyfluorescein, but does it really has a long fluorescent
life-time and/or are there any better secundary antibodies?
Thank you in advance,

Sven Terclavers



From daemon Thu Sep 5 08:04:21 2002



From: JHoffpa464-at-aol.com
Date: Thu, 05 Sep 2002 08:52:17 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


floging could be part of you ISO-9000, if you include a proper porceedure.


From daemon Thu Sep 5 09:26:37 2002



From: Mike Peters :      petersmc-at-stanford.edu
Date: Thu, 05 Sep 2002 07:17:33 -0700
Subject: Re: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings
With 8500 cells it sounds like the pathologists lost them while processing
the tissue (fixation, dehydration and infiltration). One way to circumvent
this is to "embed" the recovered cells in a pellet of albumin and
crosslinking the albumin with glutaraldehyde. Basically, you gently
centrifuge the cells of interest (after fixation and osmication) down
(6-800xG) in an eppendorf and resuspend in a tiny volume of buffer (say
20uL). Then, at the same time, add 50uL of 5% BSA and 50uL of 2%
glutaraldehyde. The glut fixes and cross links the BSA with the cells of
interest embedded within. Kind of like a cocoon. This is then spun down
and can then be transferred from the eppendorf, dehydrated and embedded in
EPON. It turns the 8500 cells into a tissue chunk. The BSA serves as a
"carrier" that impedes the steady loss of cells after each sequential
step. I was taught this technique back in the late 80's by a very well
respected microscopist by the name of Wille or Willy. Look for references
from UIC back in the 80's. Oh, the BSA doesn't interfere with images
because it is not osmicated. I plan on doing some EM on FACS samples later
this fall. Let me know what you decide to do or if I can be of further
assistance.

Mike



At 08:04 PM 9/4/2002 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Sep 5 09:36:14 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 05 Sep 2002 07:32:02 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:39 AM 9/5/2002, you wrote:
} Hi XLrs,
}
} I frequently get "Unable to Allocate Memory" errors when trying to save
} images from MCTRL. Restarting Windows (NT) is the only way I have
} discovered will fix it. Anyone know what is going on?
} --
} ---
} Regards, Cameron.

This is highly suspicious of a defective app... not WinNT. Unlike
Win98, WinNT 4 & 5 handles memory totally differently, and very
well. If the app does a malloc() and does not release the memory
allocated, it is hung up. WinNT cannot perform garbage collection.
I would suggest reporting this to the vendor as an app bug.

gary g.



From daemon Thu Sep 5 09:36:14 2002



From: Materials Research Laboratories, Inc. :      mrllab-at-raex.com
Date: Thu, 05 Sep 2002 10:31:12 -0400
Subject: Re: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 9/4/02 8:44 AM, Peter Tomic at PTomic-at-anadigics.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
A few additional comments - be sure to run a standard first for the Si.
Peak broadening would be expected at the lower Sr location (+ the Si), so if
you have a decent program, the separation can usually be done. As the others
mentioned, the higher energy lines will confirm the Sr - you did not mention
in your note if quant was desired; it sounds as though it is not necessary.
If just the presence of the Sr is of interest, use those heavier lines...as
Peter suggests, make sure the operating voltage is no lower than
25KeV..You'll need roughly double the energies to get your signal on these.
One other question..you say 'on' (the Si). Knowing the depth of the Sr is
important, too.

on 9/4/02 8:44 AM, Peter Tomic at PTomic-at-anadigics.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Bill;
}
} It's true that Si & Sr overlap at ~ 1.8 KeV. The Sr major La1 line is at
} 1.806 KeV and the Si Ka1 major line is at 1.739 KeV. However, Sr is a much
} more complex atom and has a Ka1 major line at 14.164 KeV as Bill Giles
} mentioned below. Sr should be easily discernable from Si. Of course you
} will have to have an incident electron energy of about 1.5 to 2X of the Sr
} Ka1 energy to see it. I assume you can run your survey at 25 to 30 KeV?
}
} Peter Tomic
} Anadigics, Inc.
}
}
}
} -----Original Message-----
} } From: Giles, Bill [mailto:William.Giles-at-timet.com]
} Sent: Tuesday, September 03, 2002 4:29 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE: Strontium on Clinoptilolite
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Try looking at 14.164 Kev, should be a K line down there.
}
} William Giles
} Senior Electron Microscopist
} Metallography Lab Coordinator
} P.O. Box 2128
} Henderson, Nevada 89009
} (702) 566-4436
} (702) 564-9038 (Fax)
} Bill.Giles-at-timet.com
}
} RE:
} Hello all !
} Maybe somebody knows how to analyse samples of clinoptilolite containing
} strontium on EDX detector. Strontium and Silicon have both peak in one line.
} Please help me. This is my doc work.
}
}
}
} *
}
}




From daemon Thu Sep 5 11:58:39 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 5 Sep 2002 11:49:27 -0500
Subject: RE: trainee

Contents Retrieved from Microscopy Listserver Archives
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Over the years I have taught a score of students to use an
ultramicrotome. I have evolved my approach so that I know start with
a trimmed block that is mounted and ready to cut. Once they get
sections, I back up the knife and start with the the simplest
alignment along the y axis, then i move on to x-axis and finally
z-axis alignment. when they get good at that, i switch to blocks
that need progressively more trimming. I have learned that the final
steps in microtomy are really the simplest if everything is perfect.
When students understand where they are going, it is easier for them
to learn the steps on how to get there. For example, it is hard for
them to know how much to trim off or why a small block face is better
if they have never seen a good block size or tried to thin section
one that is too big.


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu Sep 5 13:16:00 2002



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Thu, 5 Sep 2002 14:06:43 -0400
Subject: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
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Seth,
I have sectioned cell fractions by putting the sample into a clean
polypropylene eppendorf tube, centrifuge it well and do the total
fixation -} embedding without disturbing the "pellet". Care must be
taken to totally exchange the solutions. Repeat a step if there is a
question. After the resin (epon) is hard the next morning, pop it
out of the tube (I usually fill 1/4 of the tube with the final resin).
The pellet should be visible on the side of the cone at the bottom -
try using a dissecting microscope with the light under the the block.
It is usually necessary to cut the cone to a flat side and glue it
onto a blank so that it can be sectioned.
Pat

Patricia Stranen Connelly
The University of Pennsylvania
Department of Biology
Philadelphia, PA 91904-6018

215-898-7145
psconnel-at-sas.upenn.edu


From daemon Thu Sep 5 14:30:23 2002



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Thu, 5 Sep 2002 15:20:50 -0700
Subject: Re: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Seth-
This is a small number for EM preparation. If I were you, I would
attach them to the bottom of one well in a 48-well plate using
poly-lysine to afix them. Then, you could embed them by making a
cast of the dish in epoxy resin. You need somebody reasonably adept
in the lab to do this, but it isn't hard.
Carol Heckman
(Bowling Green State University)



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Sep 5 14:33:29 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 5 Sep 2002 15:32:19 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To be ISO compliant, one must have the proper documentation in place as well
as a gaggle of ISO type people to do the audits.

-----Original Message-----
} From: "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com
[mailto:"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com]
Sent: Thursday, September 05, 2002 8:52 AM
To: gary-at-gaugler.com; landers-at-jhmi.edu
Cc: Microscopy-at-sparc5.microscopy.com


floging could be part of you ISO-9000, if you include a proper porceedure.


From daemon Thu Sep 5 14:44:55 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 05 Sep 2002 12:40:24 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm working with a Sirion XL30 with Edax. MCTRL is running under
WinNT4 and is integrated with Soft Imaging analySIS. Host
computer is a Umax server. Never had a crash or memory
problem or had to reboot.

I still think it is a MCTRL version problem. And, any system
running Win 3.1 is an antique. If it is under contract, I would
have expected FEI to upgrade the system (they would have to
provide a new computer box to do this effectively since WinNT
is set up for the PCI cards. The 3.1 system is most certainly
ISA cards.) They probably don't want to put out the cost of
upgrading.

I upgraded my Amray SEM control from Win 3.1 to Win95 and
then to Win98SE. I used an ASUS motherboard with Pentium II
450MHz, 384MB RAM, dual 40G drives, Zip, floppy and
SCSI Jaz drive. The motherboard has one ISA slot which I
needed for the Nibblenet interface card. All of the other
cards are PCI. The Robinson BSE control program works fine
on any of the Win OS versions. Fortunately, it communicates
via serial port.

gary g.


At 11:48 AM 9/5/2002, you wrote:
} I had the same problem with my XL. FEI gave me no answers. I believe it
} has something to do with the EDAX integration. I got the error after heavy
} use of the EDAX software.
}
} Jon
}
} Gary Gaugler wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } At 04:39 AM 9/5/2002, you wrote:
} } } Hi XLrs,
} } }
} } } I frequently get "Unable to Allocate Memory" errors when trying to save
} } } images from MCTRL. Restarting Windows (NT) is the only way I have
} } } discovered will fix it. Anyone know what is going on?
} } } --
} } } ---
} } } Regards, Cameron.
} }
} } This is highly suspicious of a defective app... not WinNT. Unlike
} } Win98, WinNT 4 & 5 handles memory totally differently, and very
} } well. If the app does a malloc() and does not release the memory
} } allocated, it is hung up. WinNT cannot perform garbage collection.
} } I would suggest reporting this to the vendor as an app bug.
} }
} } gary g.



From daemon Thu Sep 5 16:46:01 2002



From: paul r hazelton, PhD :      paul_hazelton-at-umanitoba.ca
Date: Thu, 05 Sep 2002 16:37:06 -0500
Subject: Re: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


having had to visualize pellet sizes of below 1000 peripheral blood
monocytes in one earlier study of chlymidial infection, i can only say
that i had mixed success in looking for essentially non-visable
pellets. it may be a bit harsh to suggest that the pathologists simply
lost the cells during processing. even after embedding in either
agarose or albumin, the pellets, or embedded cores, will probably be
only marginally visable, at best.



From daemon Thu Sep 5 18:23:54 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 05 Sep 2002 16:17:30 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What is the computer system configuration? CPU, speed,
physical memory, motherboard, disk, etc?

gg

At 03:03 PM 9/5/2002, you wrote:
} Thought it might be appropriate to share our experience with the Memory
} Allocation Error............
}
} Our XL-40 was installed 3/97 as a stand alone 3.11 system. In 4/98 we
} completed conversion to NT 4.0 then in 9/98 embedded our EDS system. Very
} shortly thereafter (1 week), we began getting the dreaded Memory
} Allocation Error. The error occured with varying frequency, ranging from
} twice a day to twice a month. We found that a full PC shut down was
} required to clear the error. Over the years we tried many things to get
} rid of this error; to no avial. Following is a list of things we tried
} which did NOT appear to affect the frequency of the error:
}
} 1. save images directly to the C drive
} 2. save images directly to a network drive
} 3. added more memory
} 4. upgraded to XL v. 5.7
} 5. upgraded to service pack 4.0
} 6. changed out the PC (new Acer 11000)
} 7. changed out the hard drive
} 8. upgraded to service pack 5.0
} 9. upgraded to service pack 6a
} 10. installed multi user login shell (account log)
}
} We found experimentally that the error could be induced by alternately
} saving images and EDS spectra (about 20 each would usually do it). In
} early May of this year, we acquired a new EDS-WDS system, which was
} installed stand-alone (not embedded). We have not had a Memory Allocation
} Error since!
}
} For what it's worth....................
}
} Karen Dye
}
}
} } } } begg.4-at-osu.edu 09/05/02 04:39AM } } }
} Hi XLrs,
}
} I frequently get "Unable to Allocate Memory" errors when trying to
} save images from MCTRL. Restarting Windows (NT) is the only way I
} have discovered will fix it. Anyone know what is going on?
} --
} ---
} Regards, Cameron.



From daemon Thu Sep 5 19:43:33 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Thu, 05 Sep 2002 20:35:18 -0400
Subject: MicroZ-II XEDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Second try

} } Hi there guys,
} }
} } I have a Tracor Northern MicroZ-II XEDS detector sat in my lab that I
} } thought I might once be able to use. It has been kept cold since I
} } inherited it about 6 years ago and I finally have to admit that I am never
} } going to use it. So the question is, does anyone want it? It is a model
} } D-1830 with the serial number 02880935. It has a microscope flange attached
} } to it but I am unsure of the instrument that it came from.
} } "Buyer" collects or pays shipping and no whining about it not working, it is
} } as is. I haven't ever used it and so I have no idea if it works. The guy
} } who gave it to me assured me that it worked when it came off his SEM but as
} } I say that was 6 years ago.
} }
} } Any interest? Otherwise it goes to UM Property Disposition!
} }
} }
} } --
} } John Mansfield PhD MInstP
} } North Campus Electron Microbeam Analysis Laboratory
} } 417 SRB, University of Michigan
} } 2455 Hayward, Ann Arbor MI 48109-2143
} } Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} } (Leaving a phone message at 936-3352 is preferable to 834-3913)
} } Email: jfmjfm-at-engin.umich.edu
} } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} } Location: Lat. 42! 16' 48" Long. 83! 43' 48"
} }



From daemon Fri Sep 6 07:55:10 2002



From: JHoffpa464-at-aol.com
Date: Fri, 06 Sep 2002 08:43:41 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


i love big block faces, 3mm wide are the best.


From daemon Fri Sep 6 07:55:21 2002



From: JHoffpa464-at-aol.com
Date: Fri, 06 Sep 2002 08:48:32 -0400
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


you computer is over 5 years old, it's time to upgrade, by that i maean a whole new computer. with prices the way they are today for pcs it should be painless.


From daemon Fri Sep 6 10:49:13 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Fri, 06 Sep 2002 11:36:26 -0400
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That is all very well, but when you computer runs a microscope that is under
service contract and it is a microscope that is in use 20 hours per day,
then upgrading it is not just a question of going to Best Buy and getting
the latest e-Machines piece of you know what. FEI will not specify that
their system will run with the latest computers and you also need one with
an ISA slot and one that supports the video card they use to work with the
overlay card that exists in the ISA slot. (I am talking legacy equipment
here, the newer FEI systems use all PCI and have different configurations).
Most of you know that having a service contract on the instrument permits
you to have a gun change (a ten or so thousand dollar fix), if you have a
tip go bad in a field emission instrument (your $26,000 a year service
contract covers that). But it does not cover replacing the computer when it
becomes so slow in comparison with the other systems in the lab that you
think you are working on a Sinclair Spectrum!

On 9/6/02 8:48 AM, ""JHoffpa464-at-aol.com"-at-sparc5.microscopy.com"
{"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} you computer is over 5 years old, it's time to upgrade, by that i maean a
} whole new computer. with prices the way they are today for pcs it should be
} painless.
}
}

--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"




From daemon Fri Sep 6 10:49:13 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 06 Sep 2002 10:05:35 -0500
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I hope you all understand that Microsoft isn't exactly telling the truth
when it pops up a memory allocation error. Okay, maybe it is telling the
truth, but the question is what truth?

Memory errors can pop up due to insufficient RAM on the computer (the kind
you can add more of), insufficient GDI or USER space (more about that
later), or even insufficient disk space (usually not a problem and easy to
correct). I have seen all three labeled as "memory" errors and usually
without a lot of hint as to which kind it was. The first and third issues
are fairly easy to verify. The second can be more of a challenge.

For the first, you can hit Ctrl-Alt-Delete under Windows NT or 2000 and
bring up the Task Manager to see how much memory is actually in use and how
much remains free. Windows 9x (and 3.x, I think) have a utility program
called SYSMON that can be installed to provide the same information. Both
types of Windows provide a swap file on disk to virtually extend your
memory beyond what can be held in the actual chips. It is slower to use the
swap file than real memory, but it should allow your system to keep running
even when it is allocating far more memory than is provided by the chips.

For the third, you should be able to open up a Windows Explorer window and
see how much free space you have left on your disk(s). Admittedly, this is
not the usual meaning of a memory error. (Disks are cheap now so there is
USUALLY an abundance of space. However, older Windows 3 machines might be
cramped for space.) However, I ran into one obscure program that needed
workspace on a hard drive and the drive that workspace was on was filling
up. The program itself, rather than Windows, coughed up the insufficient
memory problem. But Windows could generate such an error if disk space is
running low and Windows would like to extend the swap file but can't due to
a lack of space.

The second issue could be more troublesome to track down but even more of a
limitation. Windows allocates a small amount of memory, 64 kilobytes (not
megabytes), to various bookkeeping tasks. Under Windows 3, a total of 64 kB
was split between GDI (graphics stuff) and User (other various uses)
stacks. Microsoft realized that wasn't enough so they gave each their own
64 kB chunk under Windows 9.x - but that can still be rather stingy. They
also provided a tool, RSRCMTR.EXE, to track the use of that memory.
(Rsrcmtr SEEMS to show a third type of resource, System, but that is really
only the smaller of GDI and User.) Note that this small amount of memory is
set aside by Windows and it doesn't matter if your machine is loaded with
512 MB of SDRAM. You only get 64 KB each for GDI and User memory!!
(I would like to say something about Windows NT here, but I have recently
made the transition and cannot authoritatively say how they handle the
issue. It might be better.)

BTW, when GDI and/or User resources are depleted, any number of strange
symptoms can appear: icons can lose their appearance, icon names can
disappear or get corrupted, dialog boxes can turn illegible, etc.
Basically, you will notice.

As programs are loaded, GDI and User and regular memory will get consumed.
Some ill-written programs can allocate any or all of these three types of
memory and forget to free them up when they are done with them. This can
happen as the program runs continuously and documents are opened and
closed, or as the program is repeatedly started and stopped and restarted.
I have encountered many such programs over the years - even earlier
versions of Excel and Eudora were notorious for such behavior (chewing up
GDI and/or User memory). If it is a problem, it falls to the author to root
out the bug and fix it, and most authors do just that.

All that said, I don't know which flavor of memory problem you have.
Hopefully, you can determine that for yourself from my comments above and
pin down the problem. If you have further questions, feel free to ask.

Now I would like to hear from any NT gurus out there - what are the rules
for GDI and User space under NT and is there a good utility for tracking
their level? I have recently jumped to Windows 2000 and could find myself
facing the same issues as the original poster.

Warren

At 04:17 PM 9/5/02 -0700, you wrote:

} What is the computer system configuration? CPU, speed,
} physical memory, motherboard, disk, etc?
}
} gg
}
} At 03:03 PM 9/5/2002, you wrote:
} } Thought it might be appropriate to share our experience with the Memory
} } Allocation Error............
} }
} } Our XL-40 was installed 3/97 as a stand alone 3.11 system. In 4/98 we
} } completed conversion to NT 4.0 then in 9/98 embedded our EDS
} } system. Very shortly thereafter (1 week), we began getting the dreaded
} } Memory Allocation Error. The error occured with varying frequency,
} } ranging from twice a day to twice a month. We found that a full PC shut
} } down was required to clear the error. Over the years we tried many
} } things to get rid of this error; to no avial. Following is a list of
} } things we tried which did NOT appear to affect the frequency of the error:
} }
} } 1. save images directly to the C drive
} } 2. save images directly to a network drive
} } 3. added more memory
} } 4. upgraded to XL v. 5.7
} } 5. upgraded to service pack 4.0
} } 6. changed out the PC (new Acer 11000)
} } 7. changed out the hard drive
} } 8. upgraded to service pack 5.0
} } 9. upgraded to service pack 6a
} } 10. installed multi user login shell (account log)
} }
} } We found experimentally that the error could be induced by alternately
} } saving images and EDS spectra (about 20 each would usually do it). In
} } early May of this year, we acquired a new EDS-WDS system, which was
} } installed stand-alone (not embedded). We have not had a Memory
} } Allocation Error since!
} }
} } For what it's worth....................
} }
} } Karen Dye
} }
} }
} } } } } begg.4-at-osu.edu 09/05/02 04:39AM } } }
} } Hi XLrs,
} }
} } I frequently get "Unable to Allocate Memory" errors when trying to
} } save images from MCTRL. Restarting Windows (NT) is the only way I
} } have discovered will fix it. Anyone know what is going on?
} }
} } Regards, Cameron.

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Fri Sep 6 10:49:36 2002



From: Doug Keene :      drk-at-shcc.org
Date: Fri, 06 Sep 2002 08:37:44 -0700 (Pacific Daylight Time)
Subject: corrective eye surgery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists,

I hope to hear from anyone with good or bad personal
experience with lasik or other corrective eye surgery. I
am particularly anxious to know if the surgery adversely
affected vision through microscope binoculars, and the
difficulties with loosing near vision (I take my glasses
off for close work).

Many thanks,

Doug

----------------------
Douglas R. Keene
Micro-Imaging Center
Shriners Hospital for Children
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97239
Phone: 503-221-3434
FAX: 503-412-6894



From daemon Fri Sep 6 11:31:11 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Fri, 06 Sep 2002 09:23:49 -0700
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I always used to start by teaching them how to make glass knives. This
conveys the idea that we're dealing with very small things that need
enormous attention to detail. From there, I used to show them how to process
tissue, then how to trim a block and then move on to thick sections, thin
sections, staining, and finally using the microscope. The continuity of
following their own work from beginning to end made it more interesting, and
therefore memorable, for them. I also took care to explain why we needed to
do each individual action, and what would happen if we didn't. I gave each
one a copy of my lab manual, but I also insisted that they add their own
thoughts as we proceeded.
All this is in the past tense because I took early retirement as soon as
I could. This, along with endless patience and a sense of humour, is also
necessary.

Lesley Weston.



From daemon Fri Sep 6 11:39:02 2002



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Fri, 06 Sep 2002 09:29:44 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well said, John. This is an issue that has been creeping up on us since
microscopes became intimately attached to computers. My 1983 Philips 410LS
runs pretty well with no RS232 and a stand-alone Gatan CCD. But my 1998
Hitachi SEM is a bit slow since it is tied to Windows 95. Hitachi informs
me that an upgrade to Windows NT 4.0 (that's right; not even Windows 2002
or XP) will cost close to $20K. We benefit from the versatility of a
computer interface but the manufacturer benefits from selling machines that
have the life span of a computer.

Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
http://katie.ucdavis.edu
raharris-at-ucdavis.edu


At 11:36 AM 9/6/2002 -0400, John F. Mansfield wrote:
} ----------------------------------------------------------.
}
}
} That is all very well, but when you computer runs a microscope that is under
} service contract and it is a microscope that is in use 20 hours per day,
} then upgrading it is not just a question of going to Best Buy and getting
} the latest e-Machines piece of you know what. FEI will not specify that
} their system will run with the latest computers and you also need one with
} an ISA slot and one that supports the video card they use to work with the
} overlay card that exists in the ISA slot. (I am talking legacy equipment
} here, the newer FEI systems use all PCI and have different configurations).
} Most of you know that having a service contract on the instrument permits
} you to have a gun change (a ten or so thousand dollar fix), if you have a
} tip go bad in a field emission instrument (your $26,000 a year service
} contract covers that). But it does not cover replacing the computer when it
} becomes so slow in comparison with the other systems in the lab that you
} think you are working on a Sinclair Spectrum!
}
} On 9/6/02 8:48 AM, ""JHoffpa464-at-aol.com"-at-sparc5.microscopy.com"
} {"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } you computer is over 5 years old, it's time to upgrade, by that i maean a
} } whole new computer. with prices the way they are today for pcs it should be
} } painless.
} }
} }
}
} --
} John Mansfield PhD MInstP
} North Campus Electron Microbeam Analysis Laboratory
} 417 SRB, University of Michigan
} 2455 Hayward, Ann Arbor MI 48109-2143
} Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} (Leaving a phone message at 936-3352 is preferable to 834-3913)
} Email: jfmjfm-at-engin.umich.edu
} URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} Location: Lat. 42! 16' 48" Long. 83! 43' 48"



From daemon Fri Sep 6 12:43:44 2002



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Fri, 6 Sep 2002 13:34:53 -0400
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Now I would like to hear from any NT gurus out there - what are the rules
} for GDI and User space under NT and is there a good utility for tracking
} their level? I have recently jumped to Windows 2000 and could find myself
} facing the same issues as the original poster.

Any good programmer should be able to identify a leaking program. Tracking
the leak back to the source code can be time consuming but is not
impossible. Checking for memory leaks is (or should be) part of any
software program testing procedure.


Here's a good starting place for Win32 programs.

http://msdn.microsoft.com/msdnmag/issues/01/03/leaks/leaks.asp

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Fri Sep 6 13:20:36 2002



From: Albert Coritz :      cactusgrower-at-earthlink.net
Date: Fri, 6 Sep 2002 14:12:56 -0400
Subject: RE: corrective eye surgery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Doug:

I had Lasix in February with spectacular results, it was worth every penny
I spent. I have 20/20 distance vision now plus good vision up close (8
inch WD) for fine work. I have some slight "Starburst" refraction at night
but it is does not effect my night vision. I am active in Astronomy too
with a large telescope at my disposal and notice no detrimental effects
from the surgery. As for the work at the Microscope or Microtome I think
it is much better than before I have the corrective surgery.

The most important thing is to choose a Lasix center that does the
procedure under "real" operating room conditions vs clean room environments
like some of the "Vi son Mills" in Malls have. I took my time and shopped
around not for the best price but the best center & surgeon.

--- Albert Coritz
--- cactusgrower-at-earthlink.net
Sales Manager EMS/ Diatome USA


} [Original Message]
} From: Doug Keene {drk-at-shcc.org}
} To: {Microscopy-at-sparc5.microscopy.com}
} Date: 9/6/2002 11:37:44 AM
} Subject: corrective eye surgery
}
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}
}
} Hello Microscopists,
}
} I hope to hear from anyone with good or bad personal
} experience with lasik or other corrective eye surgery. I
} am particularly anxious to know if the surgery adversely
} affected vision through microscope binoculars, and the
} difficulties with loosing near vision (I take my glasses
} off for close work).
}
} Many thanks,
}
} Doug
}
} ----------------------
} Douglas R. Keene
} Micro-Imaging Center
} Shriners Hospital for Children
} 3101 S.W. Sam Jackson Park Road
} Portland, Oregon 97239
} Phone: 503-221-3434
} FAX: 503-412-6894
}




From daemon Fri Sep 6 15:47:30 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 6 Sep 2002 15:37:41 -0500
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
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Scott,
That's fine if you are the software developer. I am a little
old academic using programs like MS word, netscape, photohop, acrobat
reader, and a few others on my computer. Nothing too wild. Well these
programs definitely leak memory. Once in a while I must restart the
computer after getting bogus memory errors. I suspect the Microsoft
products. But I havn't found any way for an ordinary user such as
myself to figure out *which* program is actually leaking.

Tobias

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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Sep 6 21:57:04 2002



From: Alan Davis :      adavis-at-saipan.com
Date: Thu, 5 Sep 2002 20:48:49 +1000
Subject: photo strobe in microscope base?

Contents Retrieved from Microscopy Listserver Archives
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I would be interested in hearing from anyone who has put a photographic strobe into a microscope stand, on the cheap, for better photos. Reflected light on Stereomicroscope is obvious; I am thinking of kohler illumination. My limited experiments, wven with a digital camera, have revealed exposure times to be limiting, not to mention vibration.

Alan Davis
Marianas High School,
Saipan

--
adavis-at-saipan.com 1-670-322-6580
Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI

I have steadily endeavored to keep my mind free, so as to give up any
hypothesis, however much beloved -- and I cannot resist forming one
on every subject -- as soon as facts are shown to be opposed to it.
-- Charles Darwin (1809-1882)

The right to search for truth implies also a duty; one must not
conceal any part of what one has recognized to be true.
-- Albert Einstein

As we enjoy great advantages from the inventions of others we should
be glad of an opportunity to serve others by any invention of
ours, and this we should do freely and generously.
-- Benjamin Franklin





From daemon Sat Sep 7 13:35:17 2002



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Sat, 07 Sep 2002 13:22:39 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
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The problem of software compatibility between machines and operating system
versions is one which plagues not only users, but also manufacturers.

I began my career in this industry writing assembly code for the "minicomputers"
that were just starting to be tied to pulse-height analyzers in the early 1970s.
The challenge, of course, was to perform useful work in a 4K environment where not
even disk storage was available (and yes, I mean 4K, not 4M or 4G). When we added
peripherals, we had to write our own drivers -- and even mathematical algorithms
needed to be coded from scratch since computations generally had to be done in
"fixed point". The upside was that a programmer knew EXACTLY what was in the
computer and, after sufficient testing, could feel pretty confident about how the
code would perform under all plausible circumstances. Sure, we still introduced
our "bugs" -- and I introduced my share (as some members of this listserver can
personally testify) -- but we could figure them out by duplicating the
circumstances back in the factory. In extreme cases, you could take your debugging
right down to the individual machine instruction -- and frequently did.

Life has changed. Today, I'm sure that there is not a single human who fully
understands all of the intricacies of the Windows operating system (and some days I
think mankind in aggregate is clueless) -- not to mention all of the complexities
that occur with the addition of peripherals, utility software, and software
development tools such as compilers, linkers, library functions etc. -- look at a
typical desktop PC and there are probably thousands of people who have had a role
in establishing the integrity of the code that it is running. Sure, modern
operating systems impose barriers and structures such that programs run
independently, at least in principle, and IN PRINCIPLE, it should be possible to
freely switch between computers and add peripherals and capabilities without
incurring any problems. Some day that may be possible -- but that day hasn't yet
fully arrived.

The reality is that there remain many subtle ways in which programs can behave
properly in one environment, and not in another. With appropriate care in
programming practice, problems due to these things are rare, but when they do
occur, they are extremely difficult to isolate -- and costly, especially when they
need to be figured out in the customer's lab. Sometimes, the problem isn't even
the fault of the application programmer -- anyone who does this stuff (or manages
it) can tell you horror stories about undocumented bugs in compilers, operating
systems, and device drivers. The programmer's challenge in many cases is figuring
out how to get his/her stuff to run properly when the tools she/he must work with
aren't doing what they're supposed to.

Back in the days when instruments were "hard wired", they simply went obsolete in a
relatively short time (5 years wasn't unusual), and the user knew he/she had to
purchase a new one to get the latest/greatest features. As we have moved into a
"software" age, manufacturers have been able to provide software updates that add
features and prolong the life of the hardware. But as software environments have
grown more complicated and user expectations more demanding (and properly so),
manufacturers' software development and support costs have also grown
exponentially. Keep in mind that manufacturers of EM equipment don't enjoy the
luxury of large sales volumes, and it is easy to see that hiring a battery of
people to test software under every possible variant of computer, operating system,
and peripheral configuration just isn't feasible. So the only real practical
approach is to warrant system performance as it leaves the factory. Though most of
us would love to be nice guys and say -- "sure, just upgrade to a new computer when
you feel like it", users have a way of becoming testy and accusative when they
can't get the instrument to work properly with the new hardware (at least I am when
I find myself in this situation). One solution is to tell the user "upgrade at
your own risk" -- but this tends to generate ill will. The only realistic
alternative is for the manufacturer to take responsibility for the configuration --
and this is an expensive proposition.

So, though I appreciate and share the pain of having to buy expensive factory
upgrades (remember, we manufacturers buy stuff from other vendors and have similar
problems), I just want to point out that the presumption that the "manufacturer
benefits from selling machines that
have the life span of a computer" isn't the "gold mine" that it might appear to be
(ah -- if only that were the case!)

Fred Schamber
ASPEX LLC

Rick Harris wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Well said, John. This is an issue that has been creeping up on us since
} microscopes became intimately attached to computers. My 1983 Philips 410LS
} runs pretty well with no RS232 and a stand-alone Gatan CCD. But my 1998
} Hitachi SEM is a bit slow since it is tied to Windows 95. Hitachi informs
} me that an upgrade to Windows NT 4.0 (that's right; not even Windows 2002
} or XP) will cost close to $20K. We benefit from the versatility of a
} computer interface but the manufacturer benefits from selling machines that
} have the life span of a computer.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu
}
} At 11:36 AM 9/6/2002 -0400, John F. Mansfield wrote:
} } ----------------------------------------------------------.
} }
} }
} } That is all very well, but when you computer runs a microscope that is under
} } service contract and it is a microscope that is in use 20 hours per day,
} } then upgrading it is not just a question of going to Best Buy and getting
} } the latest e-Machines piece of you know what. FEI will not specify that
} } their system will run with the latest computers and you also need one with
} } an ISA slot and one that supports the video card they use to work with the
} } overlay card that exists in the ISA slot. (I am talking legacy equipment
} } here, the newer FEI systems use all PCI and have different configurations).
} } Most of you know that having a service contract on the instrument permits
} } you to have a gun change (a ten or so thousand dollar fix), if you have a
} } tip go bad in a field emission instrument (your $26,000 a year service
} } contract covers that). But it does not cover replacing the computer when it
} } becomes so slow in comparison with the other systems in the lab that you
} } think you are working on a Sinclair Spectrum!
} }
} } On 9/6/02 8:48 AM, ""JHoffpa464-at-aol.com"-at-sparc5.microscopy.com"
} } {"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com} wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } you computer is over 5 years old, it's time to upgrade, by that i maean a
} } } whole new computer. with prices the way they are today for pcs it should be
} } } painless.
} } }
} } }
} }
} } --
} } John Mansfield PhD MInstP
} } North Campus Electron Microbeam Analysis Laboratory
} } 417 SRB, University of Michigan
} } 2455 Hayward, Ann Arbor MI 48109-2143
} } Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} } (Leaving a phone message at 936-3352 is preferable to 834-3913)
} } Email: jfmjfm-at-engin.umich.edu
} } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} } Location: Lat. 42! 16' 48" Long. 83! 43' 48"



From daemon Sat Sep 7 19:13:49 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Sat, 07 Sep 2002 17:02:03 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
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on 9/4/02 1:43 PM, Tindall, Randy D. at TindallR-at-missouri.edu wrote:

} In training or help to train dozens of people over the years, I have run
} across one phenomenon that has always puzzled me. I first noticed this when
} showing people how to do specimen exchanges in TEM's in which a premature
} turning of the specimen arm before sufficient pumping on the airlock causes
} the high-voltage to shut down. Sometimes it would take around 20 minutes to
} get the scope back on line, and there can actually be introduction of a bit of
} oil into the column.
}
} Needless to say, I wanted to warn trainees about this, so I first started
} telling them something like "Insert the specimen arm, BUT DO NOT TURN IT
} UNTIL....." Almost invariably, this resulted in exactly what I wanted to
} avoid, usually the first or second time I wasn't there to watch. Finally, I
} switched to saying "Insert the specimen arm and push it into place with the
} flat of your hand, then after the light goes out, turn it clockwise". That
} seems to work.
}
} The point is, I guess, that in training people about things that can
} potentially damage equipment, I have found it best NOT to say "Don't ever do
} this!" It works much better (for me) to say "Do it like this." and
} demonstrate a way that minimizes the chance of an accident. Later on, after
} the habit has been established, I can explain the reasons without causing bad
} things to happen.
}
} Has anyone else experienced this? Is this Edgar Allen Poe's "Imp of the
} Perverse" or just, gulp, me?
}
Dear Randy,
Sue and I have a saying that the universe doesn't hear "not". I would
say, "Insert the specimen arm, then stop." I could then explain that one
has to wait for the vacuum to be established, etc. (In microscopes with a
vacuum-ready indicator, you could say, "...stop until the light goes on.")
On a humid day, you could explain that the electrons need a vacuum on the
inside of the column, and the trainee needs the air on the outside, so the
pumpdown is necessary to go from outside to inside, during the time you are
waiting to introduce the next step.
Yours,
Bill Tivol



From daemon Sun Sep 8 12:00:24 2002



From: David Knecht :      knecht-at-uconn.edu
Date: Sun, 8 Sep 2002 12:51:09 -0400
Subject: sticky shutter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a shutter that has become sticky opening and closing. Does
anyone have any suggestions concerning an appropriate lubricant to
apply to the leaves to try and smooth its motion. Thanks- Dave



From daemon Sun Sep 8 16:06:18 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Sun, 8 Sep 2002 19:13:47 -0400 (EDT)
Subject: Re: sticky shutter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oils should not be used to lubricate leaf shutters or iris diaphragms.
Paradoxically, instead of acting as a lubricant the surface tension of
the oil film
will bind the leaves together and prevent them from sliding freely.
Problems with sticking are likely caused by dust or a particle of
grit,
a buildup of crud somewhere, excessive wear or mechanical damage.
Careful cleaning of the shutter may be a better bet.
Chris

----- Original Message -----
} From: "David Knecht" {knecht-at-uconn.edu}
To: "microscopy" {microscopy-at-sparc5.microscopy.com}
Sent: Sunday, September 08, 2002 5:51 PM


If it is a Vincent Assoc. Uniblitz shutter, don;t apply anything to it.
Call them.

___________________________________
WORK: http://www.aecom.yu.edu/aif/


On Sun, 8 Sep 2002, David Knecht wrote:
} We have a shutter that has become sticky opening and closing. Does
} anyone have any suggestions concerning an appropriate lubricant to
} apply to the leaves to try and smooth its motion. Thanks- Dave
}
}
}



From daemon Mon Sep 9 01:42:04 2002



From: Stacey Andringa :      Stacey.Andringa-at-uc.edu
Date: Mon, 09 Sep 2002 08:17:58 -0400
Subject: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dave,

Contact Miller-Stephenson Chemical Company at (203)743-4447 or
(818)896-4714. Ask
for the MS-143V or MS-145 for brush, not the aerosol. They provide free
samples. You may specify the amount of dry lubricant in the fluid. 1% to 5%
is all you need.

Both these products are used as dry lubricants and mold release agents. Both
are solids suspended in volatile fluid. High vacuum compatible. I use those
on JEOL TEMs mechanical shutter blades (pretty large blades) under the
viewing screen, as well as on other mechanisms.

Make sure that the shutter blades are squeaky clean, before applying these
products. No dust, oil, fibers, etc.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: David Knecht {knecht-at-uconn.edu}
To: microscopy {microscopy-at-sparc5.microscopy.com}
Sent: Sunday, September 08, 2002 12:51 PM


Does anyone have a method to de-plasticize sections in Spurr resin that
can allow immunohist to be done on them?
Thanks.
Stacey Andringa



From daemon Mon Sep 9 08:27:35 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Mon, 9 Sep 2002 08:18:45 -0500
Subject: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My name is Terry Ellis I work for Hallmark Cards Inc. and I run their
SEM-EDX equipment and also use a metallurgical, stereo and regular
microscope for sample prep and various projects. I am a diabetic and have
had several eye problems and surgeries.
one, the Doctor used a laser several to tack down my retina this has
reduced my night vision some but hasn't affected my microscope use.
Two, I had a detached retina in one eye then a year later a blood
vessel burst in the other eye, both times the doctor used microsurgery to
remove the fluid in my eye and lasers to correct the problems. Thank
goodness I still can see fine with both eyes, I have had to use a hat and
dark glasses since the body fluid that replaced the old fluid gel is much
clearer and I am more sensitive to light, on the microscopes I just use
less light.
Three, I had to have the lenses in both eyes replaced due to
cataracts in them, one lenses for distance and the other lens for close-up,
I have set up the binocular microscopes for my eyes by setting one eye
piece in focus then setting the other eye piece, which has an separate
focus knob, in focus then I can use the microscopes focus knobs to bring
it all in focus, which means that no one else can use them but I have a
camera and monitor that I move and set up for each microscope when some one
else needs to see what I see.
Four, I developed glaucoma in one eye and another Doctor put in what
he called a valve in it to relieve the pressure. I have lost some vision in
that eye but have been able to adjust the binoculars so I can almost see as
good with the bad eye but I generally use just one eye since I tend to get
headaches when I use both a lot.
During the surgery's I could only see with one eye and my boss still
calls me the one eyed microscopist.
Terry Ellis
tellis2-at-hallmark.com
816-545-6573



From daemon Mon Sep 9 11:06:29 2002



From: rcmoretz-at-att.net
Date: Mon, 09 Sep 2002 15:56:36 +0000
Subject: Re: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Stacey:
The deplasticization techniques using sodium methoxide
(do a quick search at Pubmed, or check some of the
classic biological TEM books for the exact recipes) is
the only way I know to remove any of the epoxies from
samples. Whether or not you are going to have any
antigen expression left can only be determined by doing
the experiment.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
Ridgefield, CT
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Does anyone have a method to de-plasticize sections in Spurr resin that
} can allow immunohist to be done on them?
} Thanks.
} Stacey Andringa
}
}


From daemon Mon Sep 9 16:14:50 2002



From: Steve Barlow :      sbarlow-at-sciences.sdsu.edu
Date: Mon, 9 Sep 2002 13:56:00 -0700
Subject: re: trainee

Contents Retrieved from Microscopy Listserver Archives
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I have started taping myself with a digital video camera running
through a procedure such as critical point drying or preparing
formvar coated grids. I transfer this session to my Mac and use
iMovie to edit the session, then burn it to a CD-ROM. My students
are given this custom-made CD before meeting me in the lab. Since
they have now seen the procedure demonstrated in my lab using my
protocols, I quiz them on the details of the procedure, then dismiss
those who fail the quiz and have them return at a later date to quiz
again. Those who pass are then required to demonstrate the
procedure. I can then give them individualized help on anything they
have a problem with during the procedure. Upon successfully and
safely completing the procedure, the user is considered 'checked out'
on the procedure and able to use that procedure or equipment
unsupervised.

The CD enables users to review a given procedure. It is often
difficult for new trainees to make detailed enough notes that capture
all the nuances. With the CD, however, they can easily back up and
review complex sections. If they have been off cloning for the last 4
months and forgotten all the steps of a procedure, they have the
CD-ROM to review.

This approach has cut down on my demo-as-training time and is
changing how I teach my TEM protocols lab. The time I used to spend
doing demo-training is shifting more toward protocol check-out. My
first attempts using these customized CD-ROMS were enthusiastically
encouraged by my SEM students last semester.

Steve


--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/


From daemon Mon Sep 9 20:29:38 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 9 Sep 2002 21:25:46 -0400
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I hope this is not some notion of a joke. It's a bad subject to joke about.
This all sounds highly improbable. I doubt whether Ray Charles would have
this many issues with vision.

PT

-----Original Message-----
} From: Terry E Ellis [mailto:tellis2-at-hallmark.com]
Sent: Monday, September 09, 2002 9:19 AM
To: microscopy-at-sparc5.microscopy.com


My name is Terry Ellis I work for Hallmark Cards Inc. and I run their
SEM-EDX equipment and also use a metallurgical, stereo and regular
microscope for sample prep and various projects. I am a diabetic and have
had several eye problems and surgeries.
one, the Doctor used a laser several to tack down my retina this has
reduced my night vision some but hasn't affected my microscope use.
Two, I had a detached retina in one eye then a year later a blood
vessel burst in the other eye, both times the doctor used microsurgery to
remove the fluid in my eye and lasers to correct the problems. Thank
goodness I still can see fine with both eyes, I have had to use a hat and
dark glasses since the body fluid that replaced the old fluid gel is much
clearer and I am more sensitive to light, on the microscopes I just use
less light.
Three, I had to have the lenses in both eyes replaced due to
cataracts in them, one lenses for distance and the other lens for close-up,
I have set up the binocular microscopes for my eyes by setting one eye
piece in focus then setting the other eye piece, which has an separate
focus knob, in focus then I can use the microscopes focus knobs to bring
it all in focus, which means that no one else can use them but I have a
camera and monitor that I move and set up for each microscope when some one
else needs to see what I see.
Four, I developed glaucoma in one eye and another Doctor put in what
he called a valve in it to relieve the pressure. I have lost some vision in
that eye but have been able to adjust the binoculars so I can almost see as
good with the bad eye but I generally use just one eye since I tend to get
headaches when I use both a lot.
During the surgery's I could only see with one eye and my boss still
calls me the one eyed microscopist.
Terry Ellis
tellis2-at-hallmark.com
816-545-6573



From daemon Mon Sep 9 22:17:08 2002



From: David Knecht :      knecht-at-uconn.edu
Date: Mon, 9 Sep 2002 23:10:24 -0400
Subject: Microscope table

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are being asked for information about tables to put a microscope
system on in a new lab being built. I wonder if anyone has suggestions
on what to look for in a microscope table or specific brands if known.
We are not planning an air table for this system. I should note that
when Leica spec'd our new SP2 confocal, they claimed we did not need an
air table, and so far they have been proven right. Hopefully the new
building will be as stable. Right now, the microscope in question
(Zeiss axio 200) is on a heavy duty generic metal table, on which we
have placed a heavy stone benchtop slab supported by antivibration pads
(audiophile variety). That has worked OK so far, but any suggestions
given the chance to purchase something new are welcome. Thanks- Dave



From daemon Tue Sep 10 03:49:36 2002



From: moritz-andreas.meyer-at-amd.com
Date: Tue, 10 Sep 2002 10:39:54 +0200
Subject: =?iso-8859-1?Q?glass_or_ceramics_rod_for_400=B0C_experiment_in?=

Contents Retrieved from Microscopy Listserver Archives
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Hi there,

here is what I am after. We have a self-made heating stage for an SEM, that can go up to 400°C. For thermal insulation the heater is mounted on a glass rod. When trying to mount everything I have broken the rod &-[.

Does anyone know where I can order glass or ceramics rods (or any oder material tha can take 400°C). Our former supplier is not available anymore.
I need the following size: diameter 10 mm and lenght 120 mm.
Is there a supplier in germany ?

Thanks for the help.

Andreas M.


Moritz Andreas Meyer
Dipl.-Ing. (FH), MSc.
Materials Analyst
Materials Analysis Laboratory

AMD Saxony Limited Liability Company & Co. KG
Wilschdorfer Landstraße 101
M/S E23-MA
D-01109 Dresden
F. R. Germany

Tel. +49-351-277-4149
Fax +49-351-277-9-4149
moritz-andreas.meyer-at-amd.com





From daemon Tue Sep 10 07:07:24 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 10 Sep 2002 07:56:22 -0400
Subject: RE: Microscope table

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning David,

We have an Olympus FV-300 confocal system for which we initially
used tables a la carte. The arrangement took up much space and left parts
of the system inaccessible. We have been forced to move the confocal system
to a smaller room which cannot accommodate the three tables on which we had
the system spread. Ten feet of space has dictated the table/rack
configuration and selection. The table on which we are locating the
microscope is sized to take the anti-vibration slab on which the microscope
and detector/confocal box are located. Then a second table for the computer
boxes and monitor and a third for the laser rack and power supplies. I
worked directly with the primary supplier (NOT the company from which we are
buying!) to insure that the design was consistent with our needs. The
circuitous purchase route was required by the fact that the primary supplier
expected payment on delivery (from an educational establishment?) whereas
the microscope supplier knew better.
My point is that for us the space to which we moved the system
dictated the support system and its distribution.
Finally, our three tables/racks ARE being custom made and were
purchased ($2,000) through the company that supplied the confocal system
itself. Something similar can be seen on the back page of the Fluoview
brochure which can be downloaded as PDF from:

http://www.olympusamerica.com/seg_section/seg_product.asp?p=&sc=&product=133
&fl=10

Beyond the issues enumerated, I agree, that nothing special is required. I
ended up with hand-drawn plans for the pieces which I attached to the quote
as specifications.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

THINKING IS MUCH MORE DIFFICULT THAN MEMORIZING.


} ----------
} From: David Knecht
} Sent: Monday, September 9, 2002 11:10 PM
} To: microscopy
} Subject: Microscope table
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We are being asked for information about tables to put a microscope
} system on in a new lab being built. I wonder if anyone has suggestions
} on what to look for in a microscope table or specific brands if known.
} We are not planning an air table for this system. I should note that
} when Leica spec'd our new SP2 confocal, they claimed we did not need an
} air table, and so far they have been proven right. Hopefully the new
} building will be as stable. Right now, the microscope in question
} (Zeiss axio 200) is on a heavy duty generic metal table, on which we
} have placed a heavy stone benchtop slab supported by antivibration pads
} (audiophile variety). That has worked OK so far, but any suggestions
} given the chance to purchase something new are welcome. Thanks- Dave
}
}
}


From daemon Tue Sep 10 07:39:45 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Tue, 10 Sep 2002 07:32:00 -0500
Subject: really no joke

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter:
No its all true, I wish it wasn't but it is true. I have been a type
1 Diabetic since 1963. Check out Diabetes and its long term complications.
I didn't mention it but I have also been diagnosed with the start of renal
kidney failure. I am on a vegetarian diet to slow down the failure rate and
with a couple of pills it has slowed down. I also didn't mention a bad
sprain that I did about 5 months ago that didn't heal and stayed swollen
and the foot doctor said this has caused the calcium to be leached out of
my foot so he has had me on crutches for the past two months until the foot
heals and the bones in my foot recalcifly.
I posted this in reply to a question about lasic surgery and meant
to encourage the questioner to do what he needed to do. Really the fact
that I work with microscopes has been a plus since I have been able to make
up for my eye problems and still do my job to my bosses satisfaction. With
a lot of other jobs I would have been out of work.
No Joke
Terry Ellis



From daemon Tue Sep 10 08:39:20 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Tue, 10 Sep 2002 09:25:12 -0400
Subject: Re: Microscope table

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,
We have several Vibraplane air tables (nitrogen) by Kinetic Systems
which we are very
happy with.
Mike D.

David Knecht wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are being asked for information about tables to put a microscope
} system on in a new lab being built. I wonder if anyone has suggestions
} on what to look for in a microscope table or specific brands if known.
} We are not planning an air table for this system. I should note that
} when Leica spec'd our new SP2 confocal, they claimed we did not need an
} air table, and so far they have been proven right. Hopefully the new
} building will be as stable. Right now, the microscope in question
} (Zeiss axio 200) is on a heavy duty generic metal table, on which we
} have placed a heavy stone benchtop slab supported by antivibration pads
} (audiophile variety). That has worked OK so far, but any suggestions
} given the chance to purchase something new are welcome. Thanks- Dave



From daemon Tue Sep 10 08:52:33 2002



From: Anaspec Luc Harmsen :      luc-at-anaspec.co.za
Date: Tue, 10 Sep 2002 15:43:28 +0200
Subject: Thanks to all who attended ICEM15

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all
I would like to thank, on behalf of the ICEM15 committee, all those attended
the ICEM 15 conference held in Durban, South Africa last week.
We realised that getting people to come down South to South Africa would be
a first for most, but we were overwhelmed with the numbers that did attend.
It also caught the manufacturers, who hosted brilliant social functions, a
little off guard too

We have started to change the website to accommodate the "after party
pictures" and have copies of the programme etc available to you.
Please let us know if we can assist in any way.

Again thanks to all who attended and see you all in Japan in 2006.

P.S. Nestor, do you think that the photo of you and me at the meet and greet
function could be worth money?

Luc Harmsen
Marketing ICEM15
www.icem15.com






From daemon Tue Sep 10 09:42:21 2002



From: Ron L'Herault :      lherault-at-bu.edu
Date: Tue, 10 Sep 2002 10:32:32 -0400
Subject: One eyed micorocopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} }
} } I have only one functioning eye (since birth) and was asked by the
} } researcher who wanted to hire me if it would be a problem running the
} } scanning microscope. I told him I could watch TV for hours without a
} } problem. The screen of the scope was not much different so I was and
am a
} } successful SEM operator. I can't evaluate stereo images or make use of
a
} } stereo microscope but I can still use them.
} }
} } Ron
} } ----- Original Message -----
} } } From: "Terry E Ellis" {tellis2-at-hallmark.com}
} } To: {microscopy-at-sparc5.microscopy.com}
} } Sent: Tuesday, September 10, 2002 8:32 AM
} } Subject: really no joke
} }
} }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Peter:
} } } No its all true, I wish it wasn't but it is true. I have been a
type
} } } 1 Diabetic since 1963. Check out Diabetes and its long term
complications.
} } } I didn't mention it but I have also been diagnosed with the start of
renal
} } } kidney failure. I am on a vegetarian diet to slow down the failure
rate
} } and
} } } with a couple of pills it has slowed down. I also didn't mention a
bad
} } } sprain that I did about 5 months ago that didn't heal and stayed
swollen
} } } and the foot doctor said this has caused the calcium to be leached out
of
} } } my foot so he has had me on crutches for the past two months until the
} } foot
} } } heals and the bones in my foot recalcifly.
} } } I posted this in reply to a question about lasic surgery and
meant
} } } to encourage the questioner to do what he needed to do. Really the
fact
} } } that I work with microscopes has been a plus since I have been able to
} } make
} } } up for my eye problems and still do my job to my bosses satisfaction.
With
} } } a lot of other jobs I would have been out of work.
} } } No Joke
} } } Terry Ellis
} } }
} } }
} } }
} }
}
}



From daemon Tue Sep 10 09:42:22 2002



From: msteglic-at-mail.mdanderson.org
Date: Tue, 10 Sep 2002 09:37:39 -0500
Subject: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I have had good results doing immuno on Epon-Araldite embedded tissue by etching
the plastic with sodium metaperiodate using the method described in the article
"Ultrastructural Localization of Antigenic Sites on Osmium-fixed Tissues
Applying the Protein A-Gold Technique" by Bendayan and Zollinger as published in
The Journal of Histochemistry and Cytochemistry, Vol 31:1, pp101-109, 1983.




Stacey Andringa {Stacey.Andringa-at-uc.edu} on 09/09/2002 07:17:58 AM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Mannie Steglich/MDACC)


Does anyone have a method to de-plasticize sections in Spurr resin that
can allow immunohist to be done on them?
Thanks.
Stacey Andringa








From daemon Tue Sep 10 11:48:26 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Tue, 10 Sep 2002 12:40:55 -0400
Subject: Re: One eyed micorocopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The HIROX HiScope makes 3-D discernable on a flat screen with only one eye
by utilizing kineopsis instead of stereopsis. see
http://www.hirox-usa.com

Bill Miller

At 10:32 AM 9/10/2002 -0400, Ron L'Herault wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Sep 10 12:22:27 2002



From: joe fu :      jofu-at-nist.gov
Date: Tue, 10 Sep 2002 13:15:30 -0400
Subject: SEM give away

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...
After 20 some years calibrating SRM484,our VG HB-50A UHV SEM come to the
end. We are planning to dismantle the this cold FE, UHV microscope. Does
any University or non-profit organization want this baby (still function)
for free? Please contact me.

Joseph Fu
National Institute of Standards & Technology
100 Bureau drive Stop 8212
Gaithersburg, MD. 20899-8212
Tel: 301-975-3495
Fax: 301-869-0822
Email: jofu-at-nist.gov




From daemon Tue Sep 10 13:36:07 2002



From: msteglic-at-mail.mdanderson.org
Date: Tue, 10 Sep 2002 13:29:20 -0500
Subject: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






________________________

I do not use Spur's so I don't know if this will work but I have had good
results doing immuno on Epon-Araldite embedded tissue by etching the plastic
with sodium metaperiodate using the method described in the article
"Ultrastructural Localization of Antigenic Sites on Osmium-fixed Tissues
Applying the Protein A-Gold Technique" by Bendayan and Zollinger as published in
The Journal of Histochemistry and Cytochemistry, Vol 31:1, pp101-109, 1983.



Stacey Andringa {Stacey.Andringa-at-uc.edu} on 09/09/2002 07:17:58 AM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Mannie Steglich/MDACC)


Does anyone have a method to de-plasticize sections in Spurr resin that
can allow immunohist to be done on them?
Thanks.
Stacey Andringa










Stacey Andringa {Stacey.Andringa-at-uc.edu} on 09/09/2002 07:17:58 AM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Mannie Steglich/MDACC)


Does anyone have a method to de-plasticize sections in Spurr resin that
can allow immunohist to be done on them?
Thanks.
Stacey Andringa








From daemon Tue Sep 10 14:27:34 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 10 Sep 2002 15:26:10 -0400
Subject: really no joke

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My apologies to you and everyone else I may have offended. I truly did not
mean to do that and thought someone was making light of a serious medical
condition.

You must tell me what Hallmark needs an SEM for. I'm just awfully curious
as to its' application in that business.

Peter

-----Original Message-----
} From: Terry E Ellis [mailto:tellis2-at-hallmark.com]
Sent: Tuesday, September 10, 2002 8:32 AM
To: microscopy-at-sparc5.microscopy.com


Peter:
No its all true, I wish it wasn't but it is true. I have been a type
1 Diabetic since 1963. Check out Diabetes and its long term complications.
I didn't mention it but I have also been diagnosed with the start of renal
kidney failure. I am on a vegetarian diet to slow down the failure rate and
with a couple of pills it has slowed down. I also didn't mention a bad
sprain that I did about 5 months ago that didn't heal and stayed swollen
and the foot doctor said this has caused the calcium to be leached out of
my foot so he has had me on crutches for the past two months until the foot
heals and the bones in my foot recalcifly.
I posted this in reply to a question about lasic surgery and meant
to encourage the questioner to do what he needed to do. Really the fact
that I work with microscopes has been a plus since I have been able to make
up for my eye problems and still do my job to my bosses satisfaction. With
a lot of other jobs I would have been out of work.
No Joke
Terry Ellis



From daemon Tue Sep 10 17:46:14 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 10 Sep 2002 15:35:02 -0700
Subject: Re: Microscope table

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TMC makes nice floating granite stable tables using
the same principle as for their SEM stabilizing platforms.

These babies are HEAVY. The one I've used is about
6" thick....solid granite. Floats like a feather. Has an
Olympus MX-50 confocal on it.

gary g.


At 08:10 PM 9/9/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Sep 10 20:44:49 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Tue, 10 Sep 2002 20:35:17 -0500
Subject: Re:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter,

I doubt that it is a joke as it sound entirely plausable. I have some
friends who deal with numerous life threatening health problem on a daily
basis, fortunately I'm not one of them. I'm not making light of Terry
Ellis's eye issues but some of these people make this the short list of what
can go wrong. I'm not sure the human body was built on sound engineering
principles; sometimes it's a miracle that it works as well as it does for as
long as it does.

Damian Neuberger
double lung transplant recipient
}
} I hope this is not some notion of a joke. It's a bad subject to joke
about.
} This all sounds highly improbable. I doubt whether Ray Charles would have
} this many issues with vision.
}
} PT
}
} My name is Terry Ellis I work for Hallmark Cards Inc. and I run
their
} SEM-EDX equipment and also use a metallurgical, stereo and regular
} microscope for sample prep and various projects. I am a diabetic and have
} had several eye problems and surgeries.
} one, the Doctor used a laser several to tack down my retina this has
} reduced my night vision some but hasn't affected my microscope use.
} Two, I had a detached retina in one eye then a year later a blood
} vessel burst in the other eye, both times the doctor used microsurgery to
} remove the fluid in my eye and lasers to correct the problems. Thank
} goodness I still can see fine with both eyes, I have had to use a hat and
} dark glasses since the body fluid that replaced the old fluid gel is much
} clearer and I am more sensitive to light, on the microscopes I just use
} less light.
} Three, I had to have the lenses in both eyes replaced due to
} cataracts in them, one lenses for distance and the other lens for
close-up,
} I have set up the binocular microscopes for my eyes by setting one eye
} piece in focus then setting the other eye piece, which has an separate
} focus knob, in focus then I can use the microscopes focus knobs to bring
} it all in focus, which means that no one else can use them but I have a
} camera and monitor that I move and set up for each microscope when some
one
} else needs to see what I see.
} Four, I developed glaucoma in one eye and another Doctor put in
what
} he called a valve in it to relieve the pressure. I have lost some vision
in
} that eye but have been able to adjust the binoculars so I can almost see
as
} good with the bad eye but I generally use just one eye since I tend to get
} headaches when I use both a lot.
} During the surgery's I could only see with one eye and my boss still
} calls me the one eyed microscopist.
} Terry Ellis
} tellis2-at-hallmark.com
} 816-545-6573
}
}
}




From daemon Wed Sep 11 05:46:58 2002



From: EasyDownline :      besthomebiz-at-subdimension.com
Date: Wed, 11 Sep 2002 06:51:24 -0500
Subject: 1,500+ paid members in my downline in 30 days 9/11/02 6:51:24 AM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This Program put over 1500 paid members in my downline
in 30 days without much of my own efforts.

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Please Click here to request for more information {/b} {/a}



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{a href="mailto:optinlist-at-subdimension.com?subject=REMOVE" {b}
We belong to the same opt-in list. But if you wish to have your email
address REMOVE from our database please click here {/b} {/a}
{/html}




From daemon Wed Sep 11 09:02:09 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Wed, 11 Sep 2002 09:45:21 -0400
Subject: re:3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
This is a two part (unrelated) question:
1. Is there a good software package that will construct 3D images
from TEM serial sections?
2. Is artificial z stretching of very small moderately bright
flourescent spots common when 3D movies are rotated? We are
getting elongated elipses from deconvolved images (0.2 um
stage steps, proper OTF from PSF for 100X lens), rather than
spheres.

Thanks,
Mike D.



From daemon Wed Sep 11 09:02:09 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Wed, 11 Sep 2002 08:55:20 -0500
Subject: What Hallmark does with SEM-EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:
A short list of stuff that I have been asked to do.
I get asked this question a lot at meetings and such. When we owned a
plating company I used metallurgical procedures and our metallurgical
microscope to cross-section a lot of samples and to measure their
thickness'. The samples that were to thin to measure on the microscope I
used the SEM to measure the thickness., I also used the EDX system to
determine the composition of the different plating layers and core
materials on all the samples. I still do this on plated items that we now
buy from outside companies. I also have used these procedures on different
broken machine parts, plant support ( such as cooling tower tubes and
sprinkler piping with holes in them ), embossing dyes etc.
We make our own gravvure and flexo printing plates and I have used
the SEM-EDX to determine what caused plating defects in the gravvure
printing plates and to evaluate different materials and procedures used to
make flexo plates.
We have several paper, ink, adhesive and printing chemists and I
have supported their work with the SEM-EDX and light microscopes. Papers -
their coatings, fillings, and type of paper fibers all have had o be known
to get them to print right the SEM-EDX has helped a lot..
Inks - have to be matched with the right papers and printing process and
their shapes, sizes ( usually done by a different procedure ) and
composition, the SEM-EDX has been able to help determine possible problems.
I could go on a long time about papers, inks and printing but I
better stop since I have been told that I can be boring about stuff most
people don't care about.
I also use the SEM-EDX to determine particle sizes and composition of
employees dust exposure in manufacturing, and office areas. Government
regulations require special equipment when particle sizes fall below 10
microns. I also get samples of unknown bags of powder from all over - what
it is type of questions. My favorite samples are white stuff scraped from
around bathroom stools in hotels and plants that we are responsible for,
although oversprayed paint on cars parked in our parking lots are cool too.

Terry Ellis.
Hallmark Cards Inc.



From daemon Wed Sep 11 12:57:41 2002



From: kmccourt-at-nrcan.gc.ca ()
Date: Wed, 11 Sep 2002 12:44:37 -0500
Subject: Ask-A-Microscopist: Parent Needs Help with Olympus Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kmccourt-at-NRCan.gc.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
September 11, 2002 at 12:08:12
---------------------------------------------------------------------------

Email: kmccourt-at-NRCan.gc.ca
Name: Stuart McCourt

Organization: Carleton University

Education: Undergraduate College

Location: Ottawa, Ontario Canada

Question: I am writing this for my son. We have an Olympus BHB
microscope. We have been trying to get the manuals for this
instrument but so far no luck. We have may questions. Que. there is a
lens mounted below the condenser that moves with the condensor and
plugs into the microscope. What is it for and when should it be used?
Que. I have been told that the BHB uses short barrel objective lens
not long barrel since I have lenses from a Wild microscope on it and
they do not work correctly how do I know which lens to obtain? Que
the microscope has a phase contrast unit again what objectives should
I be looking at getting? I am looking a gettting a dark feild
condenser will other dark feild condensers work if they fit into the
condenser holder?

Thank you for your help

Regards Keith McCourt

---------------------------------------------------------------------------


From daemon Wed Sep 11 13:05:53 2002



From: Paul.Nolan-at-alcan.com
Date: Wed, 11 Sep 2002 13:59:16 -0400
Subject: Re: What Hallmark does with SEM-EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Remember we are microscopists too so we enjoy the boring stuff MOST people
don't care about.
I did a lot of work at the university i used to work at looking at paper.
The art conservation students had old paper with neat paints and i got to
find out what stuff the used for dyes and pigments.
It was way cool.

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



"Terry E Ellis"
{tellis2-at-hallmar To: microscopy-at-sparc5.microscopy.com
k.com} cc:
Subject: What Hallmark does with SEM-EDX?
09/11/02 09:55
AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

snip


I could go on a long time about papers, inks and printing but I
better stop since I have been told that I can be boring about stuff most
people don't care about.

Terry Ellis.
Hallmark Cards Inc.








From daemon Wed Sep 11 14:32:17 2002



From: PHILLIPS,NANCY (HP-Corvallis,ex1) :      nancy_phillips-at-hp.com (by way
Date: Wed, 11 Sep 2002 14:20:20 -0500
Subject: HP TEM Engineering Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please do not contact me about this position, but follow the instructions
below.

Nancy Phillips

Hewlett-Packard Company

The Hewlett-Packard company is a leading global provider of computing and
imaging solutions and services for businesses and homes. The company focuses
on capitalizating on the opportunities of the Internet and the proliferation
of electronic services.
HP has 85,400 employees worldwide in 120 countries. The total revenue from
continuing operations was $42,5 billion in the 1999 fiscal year.
For detailed information about HP, its products and services can be found on
the World Wide Web at http://www.hp.com.

HP is an equal opportunity employer dedicated to affirmative action and
workforce diversity.
For more information about employment opportunities at HP, visit
http://jobs.hp.com

Job Number : 810270
Job Title : TEM Engineer
Department : Research & Development

United States : Oregon - Corvallis

Job Description:

In this job you will be working in an analytical laboratory within an HP
development site. We have a world-class analytical laboratory with a wide
variety of equipment. Our purpose is to support our HP Inkjet and emerging
business partners with world-class analytical information to help solve
complex materials, process, and product issues.

This is a Transmission Electron Microscopy (TEM) engineering position to
work with a new state of the art TEM w/Field Emission, EDS, PEELS, and dual
beam FIB sample preparation facilities. You will be developing and applying
TEM, EELS, and other related applications as assigned. This will involve
client consultations, sample preparation, running analysis on the
appropriate tool/s, interpreting the results, providing a written report to
the client with a review, lead or work in project teams delivering and
documenting new applications or technologies. You will also be expected to
serve as the primary TEM technical contact and develop working partnerships
with other departments within the organization and HP. At times you will be
asked to lead teams for initiating and developing existing and/or new
technology. You will be expected to use statistical concepts to develop and
execute experiments to support R&D and manufacturing, and maintain current
knowledge of technology trends and developments in the Electron Microscopy
community, specifically as this applies to TEM techniques.


Minimum Qualification :

Ph.D in Material Science, Physics, or related disciplines.

Experience includes: two-five years TEM experience including but not limited
to: STEM, EELS (GIF, EFTEM), EDS, and Diffraction studies. With experience
on semiconductors, metals, polymers, ceramics, in the form of bulk,
thinfilms, and single crystals using a variety of sample prep techniques
including FIB. Ultramicrotomy experience desired. General knowledge of
SEM/EDS, Light Microscopy, XRD, Low dose EM and Tomography, and other
analytical techniques. Ability to think outside the analytical box, good
communicator, advanced computer skills, and self-motivated.


How do you apply ?

The quickest and most efficient way for us to process your resume is that
you submit your resume to Hewlett-Packard by using the on-line application
form. You will easily find the application form on http//www.jobs.hp.com.

please include the specific job ID number 810270 on top of your resume, or
in your cover letter when applying for this function.
If your interest in Hewlett-Packard was instigated by either a recruitment
event or an advertisement, you should also include the specific event ID#
(if it was provided to you at the event) or the advertisement ID#..
An advertisement ID# is typically part of the text within the Ad.

For information about additional job opportunities at HP, we recommend you
use our on-line keyword search engine.This tool is available on
http://www.jobs.hp.com
You will be contacted if your resume fits the current position(s).If not,
your application is put into a central database, accessible by any manager
location.
Each time we have a job opening, your information will be checked to see if
it matches the requirements for the open positions.
Your information will remain on our central database for six months. This
means that you will only have to apply to us every six months. (You will be
contacted if your resume fits the current position(s).If not, your
application is put into a central database, accessible by any manager
location).


From daemon Wed Sep 11 15:32:37 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Wed, 11 Sep 2002 15:24:46 -0500
Subject: Hallmark SEM-EDX

Contents Retrieved from Microscopy Listserver Archives
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To all;
I forgot - from Hallmarks perspective we can protect our trade
secrets and patents from disclosure or discovery and can control the timing
of when the projects need to be done based on production needs.
Terry



From daemon Wed Sep 11 16:39:30 2002



From: Zhiping Luo :      luo-at-mic.tamu.edu
Date: Wed, 11 Sep 2002 16:09:57 -0500
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

Training of new users is an interesting work. During the past year since I
moved to the Texas A&M, I have been training more than a dozen new users of
graduate students, undergraduate students and postdocs on the JEOL 2010 TEM.
My experience is to keep the training as simple as possible.

In the beginning, in order to let the new users have a basic TEM theoretical
background, they are required to read some chapters and respond to a
questionnaire. Then for the hands-on training, I use a simple protocol to
follow up and work with the new users until they feel comfortable working
alone. Usually only about 5 sessions are needed for a new user. While I
still keep myself available to assist them to solve some particular
problems.

Since such a training is simple and the users are only responsible for few
things to do with the operation, I get more and more users with the
microscope.

Best regards,

Zhiping Luo
Research Scientist
Microscopy and Imaging Center, BSBW
Texas A&M University, College Station, TX 77843-2257
Phone: (979) 845-1129 FAX: (979) 847-8933
E-mail: luo-at-mic.tamu.edu http://www.tamu.edu/mic




From daemon Thu Sep 12 07:32:23 2002



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 12 Sep 2002 08:18:25 -0600
Subject: Re: 3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
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Dear Mike,

For the 3-D reconstruction, you could try Volocity™
(Version 1.4.1, Improvision). We used it succesfully to
produce 3-D images of Toxoplasma Gondii parasites
(Ref.: Nature 2002 Aug 1;418(6897):548-52 - check
the movies in the supplemental materials).


The software is for use mainly with confocal light
microscopy, but can be adapted easily for EM. It
is quite expensive (} 20K), so only worth it if you
intend to use it a lot.

Best

Marc

Mike Delannoy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
} This is a two part (unrelated) question:
} 1. Is there a good software package that will construct 3D images
} from TEM serial sections?
} 2. Is artificial z stretching of very small moderately bright
} flourescent spots common when 3D movies are rotated? We are
} getting elongated elipses from deconvolved images (0.2 um
} stage steps, proper OTF from PSF for 100X lens), rather than
} spheres.
}
} Thanks,
} Mike D.

--
Marc Pypaert
Department of Cell Biology,
Center for Cell and Molecular Imaging and
Ludwig Institute for Cancer Research,
Yale University School of Medicine
333 Cedar Street
New Haven CT 06520
Tel : (203) 785 3681
Fax : (203) 785 7446




From daemon Thu Sep 12 07:32:23 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 12 Sep 2002 08:25:44 -0400
Subject: What Hallmark does with SEM-EDX?

Contents Retrieved from Microscopy Listserver Archives
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Terry;

Thank you for the explanation. Today I can say I learned something.

Peter

-----Original Message-----
} From: Terry E Ellis [mailto:tellis2-at-hallmark.com]
Sent: Wednesday, September 11, 2002 9:55 AM
To: microscopy-at-sparc5.microscopy.com


Hello:
A short list of stuff that I have been asked to do.
I get asked this question a lot at meetings and such. When we owned a
plating company I used metallurgical procedures and our metallurgical
microscope to cross-section a lot of samples and to measure their
thickness'. The samples that were to thin to measure on the microscope I
used the SEM to measure the thickness., I also used the EDX system to
determine the composition of the different plating layers and core
materials on all the samples. I still do this on plated items that we now
buy from outside companies. I also have used these procedures on different
broken machine parts, plant support ( such as cooling tower tubes and
sprinkler piping with holes in them ), embossing dyes etc.
We make our own gravvure and flexo printing plates and I have used
the SEM-EDX to determine what caused plating defects in the gravvure
printing plates and to evaluate different materials and procedures used to
make flexo plates.
We have several paper, ink, adhesive and printing chemists and I
have supported their work with the SEM-EDX and light microscopes. Papers -
their coatings, fillings, and type of paper fibers all have had o be known
to get them to print right the SEM-EDX has helped a lot..
Inks - have to be matched with the right papers and printing process and
their shapes, sizes ( usually done by a different procedure ) and
composition, the SEM-EDX has been able to help determine possible problems.
I could go on a long time about papers, inks and printing but I
better stop since I have been told that I can be boring about stuff most
people don't care about.
I also use the SEM-EDX to determine particle sizes and composition of
employees dust exposure in manufacturing, and office areas. Government
regulations require special equipment when particle sizes fall below 10
microns. I also get samples of unknown bags of powder from all over - what
it is type of questions. My favorite samples are white stuff scraped from
around bathroom stools in hotels and plants that we are responsible for,
although oversprayed paint on cars parked in our parking lots are cool too.

Terry Ellis.
Hallmark Cards Inc.



From daemon Thu Sep 12 07:35:52 2002



From: alicia.ortega-at-colorado.edu (by way of Ask-A-Microscopist)
Date: Thu, 12 Sep 2002 07:26:06 -0500
Subject: Ask-A-Microscopist: etch samples of polycrystalline NiTi

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (alicia.ortega-at-colorado.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
September 11, 2002 at 22:14:48
---------------------------------------------------------------------------

Email: alicia.ortega-at-colorado.edu
Name: Alicia Ortega

Organization: CU Boulder

Education: Undergraduate College

Location: Westminster, CO, USA

Question: I have been trying to etch samples of polycrystalline NiTi
to bring out grain boundaries so I can determine the grain size.
Before etching I have been preparing the samples by polishing them
down to a 0.25 micon diamond paste. The etchant I am using is
3HNO3+2H2O+1HF.

I tried leaving the etchant on for anywhere from 1 sec to 2 min, but
nothing seems to work. I was wondering if anyine knows about or has
any experience with ethcing NiTi and might know how long this
particular etchant should take?

Thanks so much for any help you can provide!

regards
Alicia Ortega

---------------------------------------------------------------------------


From daemon Thu Sep 12 07:40:28 2002



From: Kevran44-at-aol.com
Date: Thu, 12 Sep 2002 08:34:20 EDT
Subject: looking for Jeol SEM & TEM

Contents Retrieved from Microscopy Listserver Archives
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I am looking to buy JEOL used SEM & TEM. If you know of any good used
840,845,848, or newer SEM or 1200 EX , 1010, 2000 JEOL TEM or STEM please
contact Dan at.

Daniel F. Connors
321-726-0669
321-544-5754
danieleds-at-aol.com


From daemon Thu Sep 12 08:00:09 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Thu, 12 Sep 2002 08:51:49 -0400
Subject: SEM Upgrade PGT System

Contents Retrieved from Microscopy Listserver Archives
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Hi,
Does anyone out there uses Avalon 8000 or Spirit system by PGT? I am trying
to get some positives and negatives about the systems.
Or any other suggested systems that have EDS, Digital Imaging, X-ray
mapping, pc based. The upgrade for Amray 1830I with EDS and WDS detectors.

Any info is appreciated.

Pavel Lozovyy
atcsem-at-earthlink.net



From daemon Thu Sep 12 08:02:29 2002



From: zaluzec-at-microscopy.com
Date: Thu, 12 Sep 2002 07:52:48 -0500
Subject: Surplus Equipment : Re: looking for Jeol SEM & TEM:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Daniel

You can also look at the Surplus Equipment pages

http://www.msa.microscopy.com/SurplusEquipment

Nestor
Your Friendly Neighborhood SysOp




From daemon Thu Sep 12 10:40:51 2002



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Thu, 12 Sep 2002 10:32:25 -0500
Subject: re:3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Mike,
Regarding your first question; the IMOD software package comes to mind
(http://bio3d.colorado.edu/imod/). It is a free software package developed
for SGI UNIX which has also been ported to Linux (standard PC hardware) and
probably compiles on other UNIX platforms also. A place to look for other
UNIX/Linux based freeware/shareware solutions for processing
multidimensional data sets is http://www.cs.ubc.ca/spider/ladic/software.html.
MS Windows native solutions tend to be proprietary (VoxBlast is
one package which comes to mind), and I'm not familiar with most of them,
although I believe ImageJ has some image alignment algorithm plugins, and
it has a number of stack manipulation utilities. Once the images are
aligned, the stack can be processed to a number of formats depending on
what is required by your volume rendering software.
Artificial stretching of spherical fluorescent sources in the
z-axis can arise from a number of sources. The first thing that comes to
mind is the point spread function--if the sample in question is mounted in
a media with a refractive index significantly different from that which the
point spread function was determined with then spherical abberation may
distort the resultant image.
Another source of distortion can be the fluorescent sphere
itself-if the sphere is large enough, and is composed of a material of a
refractive index different from the surrounding media, the fluorescent bead
can act like a ball lens and weird images result.
Hardware can always be a culprit. If the stage position feedback
is encoded by the stepper motor only then it is difficult to tell how much
the stage is really moving. In other words, if the stepper recieves a
signal to move 2microns, and it turns 2 microns but the coupling to the
stage z rack slips, then the stage just moved less than it was supposed
to. On a couple of our microscopes the focus drive is actually a bearing
friction coupling and it is capable of slipping. The computer, however,
thinks the stage moved the correct distance, so the spacing between
successive images in a stack is off. So if the computer thinks it moved 8
microns but it only really moved 6 microns then the image will be
stretched. If a linear encoder is providing feedback to the software this
problem should be minimized.
Lastly, the rendering software needs to have the correct
information-if the spacing between individual images in a stack is somehow
entered incorrectly, the voxels may be stretched.
Hope this helps.
Regards,
Karl G.


At 09:45 AM 9/11/2002 -0400, Mike Delannoy wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Thu Sep 12 11:54:52 2002



From: ND5762u37-at-aol.com
Date: Fri, 13 Sep 0102 02:49:31 -1000
Subject: How's It Goin' ..... ?

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Importance: Normal



From: ND5762u37-at-aol.com
Date: Fri, 13 Sep 0102 02:49:31 -1000
Subject: How's It Goin' ..... ?

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America





Lowest Phone Rate In The USA.

New 1/2 Cent Per Min. Phone Card.
$5 = 882 Min. - $10 = 1,882 Min. - $20 = 3,882 Min.

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You Get Yours At 1/3 Off !

Call Fax-On-Demand # 403-934-6061 ID Code 933-409
You Can Use This Yourself Or Sell It To EVERYONE !


From daemon Thu Sep 12 12:28:33 2002



From: Louise_Harner-at-albint.com
Date: Thu, 12 Sep 2002 13:22:01 -0400
Subject: re:3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
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by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id MAA22443
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for {microscopy-at-MSA.microscopy.com} ; Thu, 12 Sep 2002 12:26:22 -0500 (CDT)



Mike -

I downloaded the demo version of "3-D Doctor" but haven't yet had time to
try it out on my images. You might want to look at their website and see if
it looks promising for your work. I think the software package runs about
$5000.
http://www.ablesw.com/3d-doctor

Other sites that might be of interest (again I haven't had time to check
these out):
http://bio3d.colorado.edu/imod/ IMOD - freeware works in LINUX or on
Silicon Graphics computer
www.isi.uu.nl/people/michael/vr.htm VolumeJ - freeware Java
(multiplatform, Windows compatible)

Neither I nor the company I work for have any financial interest in any of
the above. I've just been looking around for ways to do reconstructions.

- Louise


Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com



|---------+----------------------------}
| | Mike Delannoy |
| | {delannoy-at-jhmi.ed|
| | u} |
| | |
| | 2002/09/11 09:45 |
| | AM |
| | |
|---------+----------------------------}
} --------------------------------------------------------------------------------------------------------------|
| |
| To: microscopy listserver {Microscopy-at-sparc5.microscopy.com} |
| cc: |
| Subject: re:3d software and zstretch |
} --------------------------------------------------------------------------------------------------------------|




------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,
This is a two part (unrelated) question:
1. Is there a good software package that will construct 3D images
from TEM serial sections?
2. Is artificial z stretching of very small moderately bright
flourescent spots common when 3D movies are rotated? We are
getting elongated elipses from deconvolved images (0.2 um
stage steps, proper OTF from PSF for 100X lens), rather than
spheres.

Thanks,
Mike D.









From daemon Thu Sep 12 13:11:36 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 12 Sep 2002 11:00:44 -0400
Subject: High-pressure freezer vendors

Contents Retrieved from Microscopy Listserver Archives
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Dear List,
I will be purchasing a high-pressure freezer. I know that Balzers makes
one, but I do not know whether there are other vendors. If anyone
(including/especially vendors) has experience with either Balzers or other
brands of high-pressure freezer, would you please contact me off-list? TIA.
Yours,
Bill Tivol



From daemon Thu Sep 12 14:17:32 2002



From: Steve Beck :      becks-at-sunynassau.edu
Date: Thu, 12 Sep 2002 15:08:56 -0400
Subject: Philips EM 300 Service

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Dear Colleagues,

Can anyone suggest an independent service engineer who could perform a
routine maintenance/PM on our Philips EM 300 (we are located just east of
New York City on Long Island)? The scope is currently down with vacuum
problem that I believe is related to poor water circulation (I have cleaned
out a couple of filters in the plumbing down behind the panel in the front
leg space of the scope without success). It should be noted that our
greatest problem with service personnel is the Nassau County requirement
that the individual carry $1,000,000 in liability insurance in order to
work on campus.

Please contact me offline if you have any leads!

Regards,

Steve

Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Thu Sep 12 14:57:24 2002



From: Karin Pruessner :      kprue-at-mse.ufl.edu
Date: Thu, 12 Sep 2002 15:50:37 -0400
Subject: TEM: field cancellation

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Dear list members,

we have some problems with electromagnetic AC and DC fields in the room
where our JEOL 2010F is installed. Therefore, I am currently looking into
field cancellation systems. Installation of a Helmholtz cage has been
recommended by the company who initially did the site survey. I would like
to hear about any user's experience (positive and negative) with it and
possible alternatives. I would be especially interested, if such a system
can deal with interference from multiple and changing fields. Any
advice/comment on this would be greatly appreciated, please contact me
off-line if appropriate. Vendors suggestions are also welcome.

Karin Pruessner, University of Florida



From daemon Thu Sep 12 15:38:52 2002



From: Larry Hanke :      hanke-at-mee-inc.com
Date: Thu, 12 Sep 2002 15:29:46 -0500
Subject: Re: Ask-A-Microscopist: etch samples of polycrystalline NiTi

Contents Retrieved from Microscopy Listserver Archives
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Alicia:

We etch NiTi alloys with a solution of 10 ml HF, 25 ml HNO3,
and 150 ml water. Etching time is 15 to 30 sec. I suspect
that your solution may be more uniformly dissolving the
alloy without delineating the structure. Also, you should
etch this alloy very quickly after polishing. Natural
passivation will inhibit etching if you delay too long
between final polish and etching. A fine oxide polish may
also be needed to get a really good polish that will show a
true structure.

Good luck.
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870




From daemon Thu Sep 12 16:08:50 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 12 Sep 2002 14:03:28 -0700
Subject: Re: SEM Upgrade PGT System

Contents Retrieved from Microscopy Listserver Archives
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You may want to dig into this more deeply. If I recall,
the 1830i has integrated x-ray and does not support
a separate system. There was some special thing done
to PC10 frame buffer that caused this.

Make sure your SEM will accept a separate x-ray. If
J9 is not used or if it has the External Beam Interface
plate installed, you might have a chance.

gary


At 05:51 AM 9/12/2002, you wrote:
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From daemon Thu Sep 12 16:31:13 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 12 Sep 2002 15:18:01 -0600
Subject: RE: 3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
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Mike,

if you are intrested in trying our analySIS software with the 3D module,
please contact me offline. The module allows reconstruction from serial
sections. Check our web site for more information.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,
This is a two part (unrelated) question:
1. Is there a good software package that will construct 3D images
from TEM serial sections?
2. Is artificial z stretching of very small moderately bright
flourescent spots common when 3D movies are rotated? We are
getting elongated elipses from deconvolved images (0.2 um
stage steps, proper OTF from PSF for 100X lens), rather than
spheres.

Thanks,
Mike D.









From daemon Fri Sep 13 07:06:55 2002



From: Microshaw-at-aol.com
Date: Fri, 13 Sep 2002 07:58:51 -0400
Subject: Ben Franklin & Flash Photos

Contents Retrieved from Microscopy Listserver Archives
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} } In a message dated Thu, 5 Sep 2002 20:48:49 +1000, adavis-at-saipan.com writes:
} }
} } } As we enjoy great advantages from the inventions of others we should
} } } be glad of an opportunity to serve others by any invention
} } } of
} } } ours, and this we should do freely and generously.
} } } -- Benjamin Franklin
} } }
} }
} } Alan-
} } I'll start out by saying that Benjamin Franklin solved the
} } controversy over patenting DNA sequences by major pharmaceutical
} } firms and research companies. These companies point out that they
} } have spent millions in research and want to make proprietary their
} } DNA sequence results. I've always said, as did Franklin here, that
} } the hundred years of scientific research, and all of the government
} } funding and grants that laid the foundations of microbiology were
} } accessed at no cost by the pharmaceutical companies and research
} } companies. Should they not pay the estates of Pasteur, Koch, Semmelweis, and
} } Lister, as well as Rosalind Carter who died of cancer from her work
} } in x-ray crystallography to discover the DNA molecule? Many people
} } contributed to the eventual result published by Watson and Crick-
} } those who weighed the 4 bases that comprise DNA, those who
} } determined it's components. Ben Franklin had it right. I have no
} } problem with pharmaceutical companies or private research companies
} } c!
} harging as much as they want for the products of their research- but
} they ought not restrict the knowledge.
} }
} } Now- about a flash unit in base. Easiest way is to get a small
} } mirror and rig it just above the light in the base. Mount your
} } flash pointed at it, and trip it via camera or manually. If the
} } mirror is small enough, you'll still get enough light from the
} } base-lamp up through the condenser to focus and compose the shot.
} } The flash (set it on auto) will give you what you need on the film.
} } If you want to see how I did mine- look at
} } http://members.aol.com/moresciencestuff/microstuff.html
} } bottom of the page.
} }
} } Good luck-
} } Mike Shaw
} } New Jersey


From daemon Fri Sep 13 07:36:13 2002



From: JHoffpa464-at-aol.com
Date: Fri, 13 Sep 2002 08:25:46 -0400
Subject: Re: Philips EM 300 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


my advice call FEI service. it will cost but will be worth it in the long run.
john


From daemon Fri Sep 13 10:50:02 2002



From: Margaret M. Miller :      millermm-at-uthscsa.edu
Date: Fri, 13 Sep 2002 10:40:10 -0500
Subject: EM plate film processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I am interested in any information concerning darkroomless processing of
4489 EM film. I know of 2 processors on the market-one stand alone and the
other water connected.
Your knowledge and input on automated film processing of 4489 film is much
appreciated.

Peggy Miller
UTHSCSA



From daemon Fri Sep 13 11:38:57 2002



From: rcmoretz-at-att.net
Date: Fri, 13 Sep 2002 16:31:04 +0000
Subject: Re: Ben Franklin & Flash Photos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Actually, the name was not Carter, it was Franklin.

Roger Moretz
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } } In a message dated Thu, 5 Sep 2002 20:48:49 +1000, adavis-at-saipan.com writes:
} } }
} } } } As we enjoy great advantages from the inventions of others we should
} } } } be glad of an opportunity to serve others by any invention
} } } } of
} } } } ours, and this we should do freely and generously.
} } } } -- Benjamin Franklin
} } } }
} } }
} } } Alan-
} } } I'll start out by saying that Benjamin Franklin solved the
} } } controversy over patenting DNA sequences by major pharmaceutical
} } } firms and research companies. These companies point out that they
} } } have spent millions in research and want to make proprietary their
} } } DNA sequence results. I've always said, as did Franklin here, that
} } } the hundred years of scientific research, and all of the government
} } } funding and grants that laid the foundations of microbiology were
} } } accessed at no cost by the pharmaceutical companies and research
} } } companies. Should they not pay the estates of Pasteur, Koch, Semmelweis, and
} } } Lister, as well as Rosalind Carter who died of cancer from her work
} } } in x-ray crystallography to discover the DNA molecule? Many people
} } } contributed to the eventual result published by Watson and Crick-
} } } those who weighed the 4 bases that comprise DNA, those who
} } } determined it's components. Ben Franklin had it right. I have no
} } } problem with pharmaceutical companies or private research companies
} } } c!
} } harging as much as they want for the products of their research- but
} } they ought not restrict the knowledge.
} } }
} } } Now- about a flash unit in base. Easiest way is to get a small
} } } mirror and rig it just above the light in the base. Mount your
} } } flash pointed at it, and trip it via camera or manually. If the
} } } mirror is small enough, you'll still get enough light from the
} } } base-lamp up through the condenser to focus and compose the shot.
} } } The flash (set it on auto) will give you what you need on the film.
} } } If you want to see how I did mine- look at
} } } http://members.aol.com/moresciencestuff/microstuff.html
} } } bottom of the page.
} } }
} } } Good luck-
} } } Mike Shaw
} } } New Jersey
}


From daemon Fri Sep 13 13:07:00 2002



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Fri, 13 Sep 2002 10:53:08 -0700
Subject: Leica service on West Coast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Does anyone offer service for a Leitz Aristoplan on the West Coast? We
have an Aristoplan with the Variophot photohead that generates its own
dirt and haze in the sealed optics, and needs the focus re-lubed. I'm
tired of shipping it to Leica in New Jersey just to have the internal
surfaces lined with thumbprints and new dirt to replace the old.

Regards,
glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************


From daemon Fri Sep 13 14:18:07 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 13 Sep 2002 14:10:12 -0500
Subject: LM - uneven field in brightfield

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have an uneven field of illumination in my microscope when I do
bright field microscopy. I have tried to align the bulb to even out
the field but I am not having much luck. I am using 20x and 63x Plan
Apo objectives and the scope is in "perfect" Kohler illumination. I
know how to subtract this variation with image processing but it
would obviously be better to minimize this variation in the original.
What I am wondering is how much variability should I expect in a well
aligned, clean microscope? If one takes a digital image of a blank
field using either a 20x 0.5 NA objective or a 63x PlanApo oil NA
1.25 objective, how wide of a pixel intensity would you find
acceptable? Is there something I should be concerned with other than
the bulb alignment? TIA, Tom


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Sep 13 14:33:19 2002



From: Margaret M. Miller :      millermm-at-uthscsa.edu
Date: Fri, 13 Sep 2002 14:26:31 -0500
Subject: 4489 film processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I am interested in any information concerning darkroomless processing of
4489 EM film. I know of 2 contained, auto processors on the market-one
stand alone and the other water connected.
Your knowledge and input on automated film processing of 4489 film will be
appreciated.

Peggy Miller
UTHSCSA



From daemon Fri Sep 13 15:47:06 2002



From: Margaret Brannigan :      brannign-at-asrr.arsusda.gov
Date: Fri, 13 Sep 2002 16:41:02 -0400
Subject: TEM: Need gold conjugate source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--
Hi,

I'm trying to find a commercial source of goat anti-chicken (serum
proteins, not egg) antibody conjugated to 10 nm gold particles. I
would deeply appreciate any recommendations anyone can give me. If
you have personal knowledge of the product's quality, that would be
even better, but not necessary--at this point, I'm just trying to
find out who offers it.

Thank you,

Margaret Dienelt

Plant Pathologist/Electron Microscopist


Floral and Nursery Plants Research Unit
National Arboretum
Agricultural Research Service, USDA

Beltsville Agriculural Research Center
Bldg. 010A Rm. 248
10300 Baltimore Avenue
Beltsville Maryland 20705

(301) 504-6097



From daemon Fri Sep 13 17:36:46 2002



From: sstouden-at-thelinks.com
Date: Fri, 13 Sep 2002 17:28:12 -0500 (CDT)
Subject: Re: Ben Franklin & Flash Photos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to forword this entire email to members of a legal list
server regards copyrights and patents.

Who that has the authority could give me permission to do?

also from where did the Franklin quote originate?
sterling

On Fri, 13 Sep 2002 rcmoretz-at-att.net-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Actually, the name was not Carter, it was Franklin.
}
} Roger Moretz
} --
} Where the world is only slightly
} less weird than it actually is.
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } } } In a message dated Thu, 5 Sep 2002 20:48:49 +1000, adavis-at-saipan.com writes:
} } } }
} } } } } As we enjoy great advantages from the inventions of others we should
} } } } } be glad of an opportunity to serve others by any invention
} } } } } of
} } } } } ours, and this we should do freely and generously.
} } } } } -- Benjamin Franklin
} } } } }
} } } }
} } } } Alan-
} } } } I'll start out by saying that Benjamin Franklin solved the
} } } } controversy over patenting DNA sequences by major pharmaceutical
} } } } firms and research companies. These companies point out that they
} } } } have spent millions in research and want to make proprietary their
} } } } DNA sequence results. I've always said, as did Franklin here, that
} } } } the hundred years of scientific research, and all of the government
} } } } funding and grants that laid the foundations of microbiology were
} } } } accessed at no cost by the pharmaceutical companies and research
} } } } companies. Should they not pay the estates of Pasteur, Koch, Semmelweis, and
} } } } Lister, as well as Rosalind Carter who died of cancer from her work
} } } } in x-ray crystallography to discover the DNA molecule? Many people
} } } } contributed to the eventual result published by Watson and Crick-
} } } } those who weighed the 4 bases that comprise DNA, those who
} } } } determined it's components. Ben Franklin had it right. I have no
} } } } problem with pharmaceutical companies or private research companies
} } } } c!
} } } harging as much as they want for the products of their research- but
} } } they ought not restrict the knowledge.
} } } }
} } } } Now- about a flash unit in base. Easiest way is to get a small
} } } } mirror and rig it just above the light in the base. Mount your
} } } } flash pointed at it, and trip it via camera or manually. If the
} } } } mirror is small enough, you'll still get enough light from the
} } } } base-lamp up through the condenser to focus and compose the shot.
} } } } The flash (set it on auto) will give you what you need on the film.
} } } } If you want to see how I did mine- look at
} } } } http://members.aol.com/moresciencestuff/microstuff.html
} } } } bottom of the page.
} } } }
} } } } Good luck-
} } } } Mike Shaw
} } } } New Jersey
} }
}



From daemon Fri Sep 13 22:49:28 2002



From: Chaoying Ni :      cni-at-udel.edu
Date: Fri, 13 Sep 2002 23:39:57 -0400 (EDT)
Subject: FESEM - comments sought

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello lister,

I was told that at this past M&M meeting, both Hitachi and JEOL
demonstrated their newest FESEM models - Hitachi S-4800 and JSM-7400F. I
was wondering if any of you could make comments concerning these two or
other FESEM versions. I'm especially interested in the quality of
corresponding cold FEGs, data base management, and proprietary
technologies such as ExB, energy filter, and in-lens or semi in-lens
detectors. Both on and off line responses are welcome. Thanks in advance!

****************************************
Chaoying Ni
The W.M. Keck Electron Microscopy Facility
College of Engineering
University of Delaware
Newark, DE 19716

(302) 831-8354 (O); -2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************



From daemon Sat Sep 14 09:07:01 2002



From: Chaoying Ni :      cni-at-udel.edu
Date: Sat, 14 Sep 2002 09:52:54 -0400 (EDT)
Subject: TEM: rough edge of FEG beam crossover at high mag

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

On our recently installed FETEM, I have noticed that when the
magnification is x800k-1.5M, the beam crossover loses its smoothness of
the edge. The shape also becomes off-circular. The alignment doesn't seem
to be an issue (checked both by myself and service engineer). My question
is whether this is normal or acceptable (scope is still under warranty).
Can anyone shed some light on this? Thanks!

****************************************
Chaoying Ni
The W.M. Keck Electron Microscopy Facility
College of Engineering
University of Delaware
Newark, DE 19716

(302) 831-8354 (O); -2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************



From daemon Sat Sep 14 12:59:23 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Sat, 14 Sep 2002 13:50:09 -0400
Subject: Re: FESEM - comments sought

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I would like to see a summary of the responses, if you
would not mind.

Thank you,
Darrell




From daemon Sun Sep 15 23:58:46 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 15 Sep 2002 22:13:21 -0700
Subject: Re: Thanks to all who attended ICEM15

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Luc
It was pleasure to be at ICEM-15. I was impressed how well everything were
organized and program was great. Thanks! Sergey

At 03:43 PM 9/10/02 +0200, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Sun Sep 15 23:58:46 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 15 Sep 2002 22:00:26 -0700
Subject: Fwd: High-pressure freezer vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Bill

I am not an expert in this area, but just completed Cryo-EM course and had
chance to work on Balzers and Leica's instruments. Leica's is compact, use
a fraction of LN2 Balzers used and more easy to operate. Could not say
anything about sample's quality - I could not get them from Elanie for a
few month. I would suggest you ask for demo and compare results
side-by-side. Best wishes, Sergey

} Date: Thu, 12 Sep 2002 11:00:44 -0400
} From: Bill & Sue Tivol {wtivol-at-earthlink.net}
} Subject: High-pressure freezer vendors
} To: microscopy list {microscopy-at-sparc5.microscopy.com}
} User-Agent: Microsoft Outlook Express Macintosh Edition - 5.01 (1630)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Sep 16 05:24:38 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 16 Sep 2002 05:23:11 -0700 (PDT)
Subject: Re: Leica service on West Coast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chaoying,

Such distortion of the beam is usually caused by charging of contaminants on
the surface of the condenser aperture. Changing or cleaning the aperture may
help. When the beam diameter is very small (like the one needed for the
magnification range you mentioned) such roughness of the beam edge is
considered normal. Try switching the condenser apertures and see if there is
big difference in the beam shape between different apertures. If one of the
apertures is "dirty" there will be big difference in distortion between it
and the other apertures. If all the apertures show comparable roughness -
don't bother to change or clean them ... the situation may worsen.
The problem of condenser aperture charging is much more severe for FEG than
LaB6 guns. The FEG is much brighter source producing very small crossover
(high coherency). If you were using LaB6 in the magnification range you
mention you'll usually work with the beam in crossover.

Hope this helps.

Best regards,

Rado

---------------------------------------------------------------------
Radostin Danev, Ph.D.
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
phone: 0564-55-7813
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------

----- Original Message -----
} From: "Chaoying Ni" {cni-at-udel.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, September 14, 2002 10:52 PM


Have you considered Otto Breiner Instruments? He's
well known here in the Detroit area. Here's the
business card information:

246 Heather Heights
Monrovia, CA 91016
ph 818-357-3859
fax 818-358-1209

Stu Smalinskas
SKF North American Technical Center
Plymouth, Michigan

__________________________________________________
Do You Yahoo!?
Yahoo! Finance - Get real-time stock quotes
http://finance.yahoo.com


From daemon Mon Sep 16 07:31:44 2002



From: Emmanuelle Roux :      eroux-at-caprion.com
Date: Mon, 16 Sep 2002 08:24:35 -0400
Subject: gold conjugate anti-chicken

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Margaret,
You will find donkey anti-chicken IgY (H+L) conjugated to gold at Bio/Can Scientific (Jackson Immunoresearch); they have 6, 12 and 18 nm gold conjugates. You can reach them at one of these numbers: 800-367-5296; 610-869-4024; 800-387-8125;
or websites: www.jacksonimmuno.com or www.biocan.com
I haven't use those so I can't tell you much more than that.
Good luck!
Emmanuelle

Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620



From daemon Mon Sep 16 12:45:44 2002



From: Anna Logvinova :      alogvinova-at-buckinstitute.org
Date: Mon, 16 Sep 2002 10:35:16 -0700
Subject: Job posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Fellow or Research Associate - Morphology Core

The Buck Institute for Age Research aims to increase the healthy years of life through clinically relevant biomedical research on the biology of aging and age-associated diseases. Established as an independent, nonprofit research facility, the Buck Institute is located in Marin County north of the San Francisco and brings a new focus to the science of aging with a world-class Faculty.

Candidates with a strong commitment to understanding aging and the causes of age-related disease are sought for the technical position of a Postdoctoral Fellow or Research Associate to join its Morphology Core facility. Duties will include sample preparation using basic histology and immunochemistry techniques, image analysis, and minor equipment maintenance. Successful candidate will instruct and assist the users of the facility and will actively participate in the ongoing research projects. Applicants should have experience in the basic histology/immunocytochemistry, fluorescence and transmitted light microscopy, and some image analysis. Experience in electron microscopy will be a plus. Excellent communication and organizational skills and the ability to manage a large workload are a must. Salary will depend upon qualification and experience. Some training will be provided. We offer a competitive salary, excellent benefits, dynamic work environment, and new state-of-the-art facility. To apply, send cover letter and resume with MC/RA on the subject line of the e-mail, to hr-at-buckinstitute.org
You are welcome to contact me if you have any questions about this position.

Anna Logvinova, M.D.
Morphology Core Manager
Buck Institute
8001 Redwood Blvd
Novato, CA 94948
www.buckinstitute.org

alogvinova-at-buckinstitute.org


From daemon Mon Sep 16 12:54:17 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Mon, 16 Sep 2002 12:48:10 -0500
Subject: MM02: proceedings and reprints.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I still did not received proceedings and reprints from MM'02 meeting.
What is the status of their mailing?

Regards,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy




From daemon Mon Sep 16 13:08:35 2002



From: tbargar-at-unmc.edu
Date: Mon, 16 Sep 2002 13:01:37 -0500
Subject: Telemicroscopy for the classroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




__________________

I run the core EM facility at UNMC in Omaha, NE. and I would like to set up
some sort of teleconferencing or telemicroscopy link with the local biology
classes. The teachers and myself of course have no experience in this. I
would like to hear from anyone who has been involved in this sort of
arrangement. All help and advice will be greatly appreciated.

Tom Bargar
402-559-7347
tbargar-at-unmc.edu




From daemon Mon Sep 16 15:52:22 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 16 Sep 2002 14:05:15 -0700
Subject: Re: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chris
To test/calibrate my laser diffractometer I used to use copper/nickel
150-300 mesh sheets. We used that material, also, to punch 3 mm EM grids in
the early era of EM. Originally it comes from electronic industry where
used in 'magnetron' production. You probably could obtain similar material
from EM grids suppliers (may be expensive if special order). You could
print such 'mesh' by contact printing on hi-res film. Such sample
delivered very nice diffraction pattern. It's not such funny as Esher
figures but nice test-sample for laser diffractometer. Another idea: to use
old friend - protection technique. Make Hi-res print-out of your funny
images, use conventional film enlarger in 'opposite' way: load unexposed
film in the film-holder and project your print-out on the film. It'll take
some experimenting with focusing, illumination, exposure etc. You may also
use convention SLR 35mm camera with hi-res/hi contrast sci grade film to
take pictures. Your drawings should be large enough. I think, it's
simplest way to do so. If need details, you may contact me off-line. Good
luck, Sergey.

At 08:58 AM 9/3/02 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Mon Sep 16 18:14:00 2002



From: Walsh, Sharon :      Sharon.Walsh-at-bannerhealth.com (by way of
Date: Mon, 16 Sep 2002 18:01:59 -0500
Subject: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in purchasing a scanner as a back-up in the event our
CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
have checked on the Agfa Duoscan and found that it is no longer in
production. Is anyone using another reasonably priced scanner and if
so, which one? Any information would be greatly appreciated.

Thank-you.

Sharon Walsh
System Technical Specialist
Laboratory Sciences of Arizona
Good Samaritan Medical Center
Phoenix, Az.
(602)239-2343


From daemon Mon Sep 16 21:06:50 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 16 Sep 2002 19:00:51 -0700
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:01 PM 9/16/2002, you wrote:

} I am interested in purchasing a scanner as a back-up in the event our CCD
} camera should fail or to scan old 3 1/4 x 4 negatives, if necessary. We
} have a Philips EM208S TEM and an AMT 2K CCD camera. I have checked on the
} Agfa Duoscan and found that it is no longer in production. Is anyone
} using another reasonably priced scanner and if so, which one? Any
} information would be greatly appreciated.
}
} Thank-you.
}
} Sharon Walsh
} System Technical Specialist
} Laboratory Sciences of Arizona
} Good Samaritan Medical Center
} Phoenix, Az.
} (602)239-2343


Check out the Nikon CoolScan 8000. It will just barely
handle TEM negs. But will definitely handle 35mm and MF formats.

gary g.



From daemon Tue Sep 17 07:28:58 2002



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 17 Sep 2002 08:21:53 -0400
Subject: RE: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Microtek Artixscan 2500f is the mechanical twin of the
Agfa Duoscan T2500. It has been updated with both IEEE1394
(Firewire) and SCSI interfaces and an improved dynamic range.
The carrier design, like the Duoscan is glassless. The 4x5
carrier works well to hold 3.25x4 TEM negs. We have a PDF I am
happy to send or visit http://www.microtekusa.com/as2500f.html

The Artixscan 1100 has a similar design but with lower
optical resolution and SCSI interface only.
http://www.microtekusa.com/as2500f.html

We have customers very happy with both.

George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com



I am interested in purchasing a scanner as a back-up in the event our
CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
have checked on the Agfa Duoscan and found that it is no longer in
production. Is anyone using another reasonably priced scanner and if
so, which one? Any information would be greatly appreciated.

Thank-you.

Sharon Walsh
System Technical Specialist
Laboratory Sciences of Arizona
Good Samaritan Medical Center
Phoenix, Az.
(602)239-2343






From daemon Tue Sep 17 07:28:58 2002



From: Susan Carbyn :      CarbynS-at-agr.gc.ca
Date: Tue, 17 Sep 2002 08:12:54 -0400
Subject: Disposable Lab Coats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I thought I'd send a message for a quick response (hopefully I don't get daggers thrown at me) - with respect to disposable lab coats. I was asked about the possibility of using them, for people coming into the lab to work on equipment, or visitors wanting to observe techniques. As part of the New Safety regulations, I have to make sure people have access to proper protective clothing. It has been argued that lab coats are contaminated when a person goes into our lab, because of the arsenic, and other contaminants we use. I know this question sounds a bit ridiculous to some, but it was suggested to me, that we have disposable lab coats for students, visitors, and any one else entering the lab.

Does anyone have any helpful thoughts on this, and could provide some helpful advice?

Thanks in advance,

Susan

Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, N.S. B4N 1J5
Canada

Phone (902) 679-5535
Fax (902) 679-2311

E-Mail: carbyns-at-em.agr.ca



From daemon Tue Sep 17 07:48:22 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 17 Sep 2002 08:46:54 -0400
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




"Walsh, Sharon (by way of MicroscopyListserver)" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am interested in purchasing a scanner as a back-up in the event our
} CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
} necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
} have checked on the Agfa Duoscan and found that it is no longer in
} production. Is anyone using another reasonably priced scanner and if
} so, which one? Any information would be greatly appreciated.
}
} Thank-you.
}
} Sharon Walsh
} System Technical Specialist
} Laboratory Sciences of Arizona
} Good Samaritan Medical Center
} Phoenix, Az.
} (602)239-2343

Get an Epson 2450 with the transparency adapter. Does a great job for about
$400.
The Nikon 8000 (and Minolta Dimage Scan Multi Pro) will only do a 6cm x 9cm
portion of a
TEM negative.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Sep 17 08:13:20 2002



From: Davis Baird :      db-at-sc.edu
Date: Tue, 17 Sep 2002 08:45:38 -0400
Subject: STM/AFM: History

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

I am a philosopher/historian of science/technology, with a particular
interest in scientific instruments. I am now starting research into
the history of the development of scanning tunneling microscopy and
atomic force microscopy. I am interested in the range of issues that
involve these developments, but I am particularly interested in (and
having some difficulty finding information about) how various
commercial instrument makers picked up and developed these
technologies. Here I would like to know who did this, how they
interacted with the academic and commercial research communities, and
how innovations were exchanged between these interacting communities.

Thanks for whatever help members can provide.

Davis Baird
--
********************************************************************
Davis Baird
db-at-sc.edu
http://www.cla.sc.edu/PHIL/faculty/baird/INDEX.HTM
Editor, Techne: Journal of the Society for Philosophy and Technology
Professor and Chair
Department of Philosophy
University of South Carolina
Columbia, SC 29208 USA
(803) 777-4166
FAX (803) 777-9178
********************************************************************


From daemon Tue Sep 17 09:03:44 2002



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Tue, 17 Sep 2002 15:09:53 +0100
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sharon

Microtek supply a whole range of glassless scanners of the Agfa duoscan
type (i'm not sure if they just took over some of the Agfa Duoscan
range).

The scanners of most interest to you are probably:
Duoscan style with conventional flatbed and seperate glassless holders
for up to about 5x10 or 8x10 inches (not cms)
Microtek Scanmaker 8700 cheapest - comes as a basic model or PRO version
with better software
Microtek Artixscan 1100
Microtek Artixscan 2500f most expensive
There are more but they seem to go up to A3 or do other things I don't
want.

dedicated large format film scanner :
(will take negatives up to 4x5 inches)
Microtek Artixscan 4500t

All have some glassless holders and range from 1200 dpi up to 2500 dpi
with Dmax from 3.4 up to 3.9; colour depth is 42 bit which should
translate to 14 bit B&W. Interfaces vary from SCSI, USB and firewire.
Prices appear to be from 600 UK pounds to 3,100 UK pounds.

You could start with the website at http://www.microtekusa.com (check
out products and the 3 ranges of scanners) and perhaps run a search
engine for more background information.

I don't have a Microtek at present and I have no connection with the
company but I am probably going to get one of the cheaper ones. If
anyone has any user experience of these scanners I would be interested
in their opinions.


Good luck.

Malcolm Haswell
E.M. Unit
School of Health, Natural and Social Sciences
University of Sunderland
UK







"Walsh, Sharon (by way of MicroscopyListserver)" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am interested in purchasing a scanner as a back-up in the event our
} CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
} necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
} have checked on the Agfa Duoscan and found that it is no longer in
} production. Is anyone using another reasonably priced scanner and if
} so, which one? Any information would be greatly appreciated.
}
} Thank-you.
}
} Sharon Walsh
} System Technical Specialist
} Laboratory Sciences of Arizona
} Good Samaritan Medical Center
} Phoenix, Az.
} (602)239-2343


From daemon Tue Sep 17 09:26:13 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Tue, 17 Sep 2002 09:19:40 -0500
Subject: Ahesive for serial sections- simple solution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listies;

I recently posted a querry requesting a good source of adhesive for serial
sections of plastic blocks.

I found that scotch tape dissolved in xylene works fine.

Thanks contributors,
Tim Quinn
University of Kansas
Program Assistant/Microscopists/Lab Mgr
Ornithology Dept.
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556/785-331-4107
tquinn-at-ku.edu


From daemon Tue Sep 17 09:40:19 2002



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 17 Sep 2002 10:33:27 -0400
Subject: RE: Need gold conjugate source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Margaret,

A invaluable source for finding antibodies is Linscott's Directory. Their
address is 815 Whitney Way, Petaluma, CA 94954 (707-763-7098).
http://www.linscottsdirectory.com

They list not only the antibodies, but where to order them. There is a fee
for the catalog and/or the online directory. I highly recommend it.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

} -----Original Message-----
} From: Margaret Brannigan [SMTP:brannign-at-asrr.arsusda.gov]
} Sent: Friday, September 13, 2002 4:41 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM: Need gold conjugate source
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} --
} Hi,
}
} I'm trying to find a commercial source of goat anti-chicken (serum
} proteins, not egg) antibody conjugated to 10 nm gold particles. I
} would deeply appreciate any recommendations anyone can give me. If
} you have personal knowledge of the product's quality, that would be
} even better, but not necessary--at this point, I'm just trying to
} find out who offers it.
}
} Thank you,
}
} Margaret Dienelt
}
} Plant Pathologist/Electron Microscopist
}
}
} Floral and Nursery Plants Research Unit
} National Arboretum
} Agricultural Research Service, USDA
}
} Beltsville Agriculural Research Center
} Bldg. 010A Rm. 248
} 10300 Baltimore Avenue
} Beltsville Maryland 20705
}
} (301) 504-6097
}


From daemon Tue Sep 17 09:48:46 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 17 Sep 2002 10:34:21 -0400
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} I am interested in purchasing a scanner as a back-up in the event
} our CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
} necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera.
} I have checked on the Agfa Duoscan and found that it is no longer in
} production. Is anyone using another reasonably priced scanner and
} if so, which one? Any information would be greatly appreciated.
} ***************

hi Sharon,
I have an EPSONExpression 1600 Pro with a transilluminator. I have
had good results with it. It has true optical resolution of 1600
dpi, was very easy to install (has Fire Wire, USB and SCSI2
interfaces). It was also VERY reasonably priced, which was good for
me because I had $4500 with which to upgrade my whole
computer/printer/scanner system. I does not have a film holder for
EM plates, but it does have a focus adjustment to account for the
film lying on the scanner bed and i have not had any trouble with
moire lines, etc.
I"m sure the more expensive scanners have better options/feature, but
this was very good for its price.
Lee

}


--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Sep 17 10:31:16 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 17 Sep 2002 10:20:25 -0500
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I like the Epson 1680 - it has a Dmax of 3.6 - you need the highest
Dmax you can get when you are working with EM negatives. Remember
this is a log scale so each 0.3 difference is 10x better. We scan in
the 16 bit mode, then adjust levels using Photoshop and then convert
to 8 bit so that we get max contrast range without all those annoying
gaps between pixel densities.


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Sep 17 13:11:40 2002



From: James Talbot :      james-at-ktgeo.com
Date: Tue, 17 Sep 2002 13:04:06 -0500
Subject: ESEM or SEM in DFW Area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List-

I have a potential client who is looking to rent time on an ESEM
(preferably) or SEM in the Dallas Fort Worth Texas (preferred) or
Houston Texas area. He has a corroded copper plug ( { 1 inch in
diameter) that he would like to get some EDS data on. If you are
interested please reply off-line.

Thank you,
James Talbot

K/T GeoServices, Inc.
Bulk and Clay Mineralogy by X-ray Diffraction
(940) 597-9076
web site: http://www.ktgeo.com/




From daemon Tue Sep 17 15:31:50 2002



From: JHoffpa464-at-aol.com
Date: Tue, 17 Sep 2002 16:21:27 -0400
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi. was interested to know if anyone has used the lynx tissue processor to stain grids. if so, any comments or suggestions.
john


From daemon Tue Sep 17 15:31:51 2002



From: JHoffpa464-at-aol.com
Date: Tue, 17 Sep 2002 16:22:30 -0400
Subject: lynx tissue processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi, was interested to know if anyone has used the lynx tissue processor to stain grids. if so, any comments or suggestions.
john



From daemon Tue Sep 17 15:32:36 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 17 Sep 2002 15:25:36 -0500
Subject: Software: speech recognition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I apologize is this is not strictly a microscopy question. A
microscopist colleague/friend of mine is interested in purchasing a
high quality speech recognition software. The individual is
handicapped and does not full use of their hands and would be using
the software for scientific writing (primarily describing microscopy
findings). Although I favor the Macintosh system (since they would
also use the computer for image processing and publishing), I am
looking for the best software to assist this individual. Any
recommendations would be appreciated. Many thanks.

JB

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Sep 17 17:17:46 2002



From: jerzy.gazda-at-amd.com
Date: Tue, 17 Sep 2002 17:08:42 -0500
Subject: TEM automated sample prep tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample preparation in our TEM laboratory and would like to hear your opinions on the following semi-automated approaches. The lab processes nearly a 1000 TEM samples per year, mostly cross-sections of CMOS devices on Si wafers. We use manual polishing, dimpling, ion mills, and FIBs tools, however we don't have enough people to handle the load and will not see an increase in headcount in the near future. I would appreciate any and all input on the following combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From root Tue Sep 17 17:47:07 2002
Return-Path: {Microscopy-request-at-sparc5.microscopy.com}
Received: (from daemon-at-localhost)
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id RAA14718
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for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Tue, 17 Sep 2002 17:13:41 -0500 (CDT)


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample preparation in our TEM laboratory and would like to hear your opinions on the following semi-automated approaches. The lab processes nearly a 1000 TEM samples per year, mostly cross-sections of CMOS devices on Si wafers. We use manual polishing, dimpling, ion mills, and FIBs tools, however we don't have enough people to handle the load and will not see an increase in headcount in the near future. I would appreciate any and all input on the following combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From daemon Tue Sep 17 19:13:24 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 17 Sep 2002 19:14:31 -0400
Subject: RE: Telemicroscopy for the classroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tom,

Short of getting instruments that are pre-pared for remote, you can
do the following.

Start at Oak Ridge to see the high end of your dreams.

http://www.ms.ornl.gov/htmlhome/mauc/default.htm

Then take a trip to BUGSCOPE:

http://bugscope.beckman.uiuc.edu/

And finally, stop off at the HOME of BugScope:

http://www.itg.uiuc.edu/technology/, where you can find
other outreach programs to emulate.

Finally, finally, a real favorite, and a very worthwhile stop:

http://www.sci.sdsu.edu/EM_Facility/index.html

Hope this helps to answer some of your questions.

Regards,

Fred Monson


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

THINKING IS MUCH MORE DIFFICULT THAN MEMORIZING.


} ----------
} From: "tbargar-at-unmc.edu"-at-sparc5.microscopy.com
} Sent: Monday, September 16, 2002 2:01 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Telemicroscopy for the classroom
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} __________________
}
} I run the core EM facility at UNMC in Omaha, NE. and I would like to set
} up
} some sort of teleconferencing or telemicroscopy link with the local
} biology
} classes. The teachers and myself of course have no experience in this. I
} would like to hear from anyone who has been involved in this sort of
} arrangement. All help and advice will be greatly appreciated.
}
} Tom Bargar
} 402-559-7347
} tbargar-at-unmc.edu
}
}
}
}


From daemon Tue Sep 17 19:29:02 2002



From: Dwight Arrieche (IIBCA) :      darriech-at-cumana.sucre.udo.edu.ve (by way
Date: Tue, 17 Sep 2002 19:17:54 -0500
Subject: LM lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear List Servers

I need some help for cleaning or removing oil from LM lenses.
What is the recommended method and solvents for this task?

Thanks

D. Arrieche
IIBCA-UDO
darriech-at-cumana.sucre.udo.edu.ve


From daemon Tue Sep 17 20:12:41 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 18 Sep 2002 13:04:42 +1200
Subject: Re: scanner (and for geological thinsections)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




This reply may not be relevant to your query, but I have recently found
out how good current domestic flatbed scanners are.

I bought a CanoScan D1250UF, the F means it has a little fluorescent
light built in to the lid, for scanning 35mm film/slides. Less than
US$200.

The holder can easily accept two pieces of polaroid film as well as a
standard petrological thinsection, and, with the thing set for 2400 dpi, in
a few minutes (less, if your computer has USB-2), you have a digital
image of the whole thinsection in transmitted light with crossed polars,
at good enough quality to blowup to A4 size!

Sure makes it easy to find your way around when the thinsection is in
the EPMA, bound to be other uses as well eg depicting textures.

cheers

rtch

}
} I am interested in purchasing a scanner as a back-up in the event our
} CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
} necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
} have checked on the Agfa Duoscan and found that it is no longer in
} production. Is anyone using another reasonably priced scanner and if
} so, which one? Any information would be greatly appreciated.
}

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Sep 18 07:26:24 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Wed, 18 Sep 2002 13:15:33 +0100
Subject: TEM automated sample prep tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jerzy,
we have a similar workload to you, but in III-V's (we have looked at
475 TEM samples since April 1). We have only 2 people (myself and one
other) doing all the sample prep, TEM and report writing. Much of our work
is straightforward thickness measurements and cross-sections, and we have no
FIB.

Do you have a Gatan PIPS or similar low-angle ion mill, with the ability
to mill from predominantly one direction? I've found a great way of making
cross sections using this machine combined with precision(ish) cleaving,
cutting out several stages from the 'standard' way of doing things. Typical
InP cross sections take less than an hour to do; with the right kind of
sample (i.e. not site specific in 2 dimensions) it is possible to stack them
up and do up to 10 at a time. It's straightforward to hit features down to
20um in size, but if you want to keep the specimen you really have to do one
at a time. (It's impossible to get several specific sites thin at the same
time, meaning you have to look at the first region that becomes electron
transparent, then destroy it while getting the next one thin, etc..) Si
takes longer since it ion mills so much more slowly, but the few I've done
still take less than 90 mins from getting the sample to looking at it in the
TEM.

I've been thinking of writing this up and publishing it somewhere, since
it has saved us a huge amount of time and effort. I'm sure that if you were
working with Si all the time you'd be able to optimise the procedure still
further. I'll try to get some words together describing the procedure, with
pictures, if you like.

FIB is great for very specific sites (less than 10um or so, depending on the
structure) and I do use one in a tame academic's lab every so often, but for
fast cross sections with lots of thin area I can't beat 'standard' methods.
However I appreciate that Si geometries are often several orders of
magnitude smaller and more complex than III-Vs, and site specific samples
are likely to be a much bigger proportion of your workload.

Good luck,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com




-----Original Message-----
} From: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Sent: 17 September 2002 23:09
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample
preparation in our TEM laboratory and would like to hear your opinions on
the following semi-automated approaches. The lab processes nearly a 1000 TEM
samples per year, mostly cross-sections of CMOS devices on Si wafers. We use
manual polishing, dimpling, ion mills, and FIBs tools, however we don't have
enough people to handle the load and will not see an increase in headcount
in the near future. I would appreciate any and all input on the following
combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with
those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White
Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





=======================================================================
This e-mail is intended for the person it is addressed to only. The
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No part of this message can be considered a request for goods or
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From daemon Wed Sep 18 07:57:57 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 18 Sep 2002 08:55:11 -0400
Subject: fish embryos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List:

Does anyone have experience fixing and embedding fish embryos in
plastics (epoxies or methacrylates) for serial sectioning? Advice,
references and warnings are all appreciated!

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Sep 18 10:34:50 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Wed, 18 Sep 2002 08:21:31 -0700 (PDT)
Subject: Re: Software: speech recognition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi John,

I purchased "Via Voice" because I wanted to use it on a Mac and of course
the choices for Mac were more limited. I also didn't want to spend a lot
of money. For me, this seems to be a farely sophisticated program for a
very reasonable price. It is, however, difficult to use if there is other
noise in the room. I can only use it in a private office.

Bob
Research Scientist
U of Washington

On Tue, 17 Sep 2002, John J. Bozzola wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I apologize is this is not strictly a microscopy question. A
} microscopist colleague/friend of mine is interested in purchasing a
} high quality speech recognition software. The individual is
} handicapped and does not full use of their hands and would be using
} the software for scientific writing (primarily describing microscopy
} findings). Although I favor the Macintosh system (since they would
} also use the computer for image processing and publishing), I am
} looking for the best software to assist this individual. Any
} recommendations would be appreciated. Many thanks.
}
} JB
}
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
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} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}
}



From daemon Wed Sep 18 10:34:51 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 18 Sep 2002 11:17:56 +0200
Subject: Philips EM 201 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

Can anyone suggest an independent service engineer who could perform a
routine maintenance on our Philips EM 201 (we are located in New Haven,
Connecticut)? The scope is currently down with a vacuum problem.

Please contact me offline if you have any leads!

Regards,

Stefan


°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6210

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Wed Sep 18 10:34:52 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Wed, 18 Sep 2002 17:24:30 +0200
Subject: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Does anyone have any good electropolishing recipes for Co-Ni alloys? We
have a Struers Tenupol. Suggestions for voltage and temperature would
be good too.

Thanks in advance


--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Wed Sep 18 10:44:28 2002



From: Oakley, Jeff :      oakleyj-at-rayovac.com
Date: Wed, 18 Sep 2002 10:37:30 -0500
Subject: book search / plating anomalies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a reference that documents various anomalies associated with electroplating and electroless plating of metals such as gold, copper, nickel, zinc, etc., as seen through the SEM.

I took a tour through Amazon's bookshelves but didn't see anything that matched what I was looking for.

TIA,

Jeff Oakley


From daemon Wed Sep 18 11:07:21 2002



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Wed, 18 Sep 2002 09:54:14 -0500
Subject: Re: LM lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Dwight,
I posted a very similar on the MSA Listserver about this time last
year. The listserv archives can be searched using the interface at
http://www.msa.microscopy.com/MicroscopyListserver/SearchMLArchive.html,
however I can't seem to get the results I'm looking for using it--I posted
a summary of the responses I received last year on 9/26.
I managed to pull up the whole exchange in my Eudora archives, so
I've appended the summary in the hope it provides you with the info you are
after. I have been using spectroscopic grade MeOH dried with 4 angstrom
molecular sieves in conjunction with cotton tipped swabs and filter
paper. Usually a confident swab in one direction across the lens while
simultaneously turning the shaft of the swab removes contamination. I use
a new swab for every wipe, and the swab is dampened with only enough MeOH
so that I can watch the trailing edge of the solvent rapidly evaporate
behind the swab as I move it across the lens. Contamination should be
trapped in the cotton tip of the swab--a similar process can be performed
using strips of lens tissue dragged across the lens if you are afraid the
swab might scratch the lens surface. I observe the whole operation with
the objective placed under a dissecting microscope so that I can see what
I'm doing, and whether it has been effective.
The lens cleaning summary follows:

Greetings,
I would like to thank all who offered their advise regarding the
cleaning of objectives, and remedies for misused optics. I had included
the suggestion of sonicating in acetone for comic relief (the rest of my
friend's suggestion was that at least then I could put a hook through the
hole where the lens used to be and use it as an ornament), however I do
appreciate the concern which was expressed by many of you. Your
suggestions have been very helpful and are much appreciated. I even learned
how to make purple Sparkle glass cleaner clear (apparently the purple dye
is photolabile).
It appears that lens cleaning is a craft, the art of which is
developed through trial and experience. There are many different
approaches to the cleaning of objective lenses, however the underlying
philosophies may be grouped under three major headings: 1.) physical
interaction with the lens surface, 2.) the use of aqueous or water soluble
cleaning solutions, and 3.) the dark art of organic solvents.
An interesting non-solvent method I have come across is to
fracture a piece of expanded polystyrene (packaging material) and to use
the freshly exposed surface to clean the lens (press into the lens and
rotate). This method was recommeded by Leitz (now Leica) in the
past. Apparently the method is effective and will not scratch the lens.
For light duty cleaning, various formulations of aqueous
solvent/detergent mixtures seem to be popular. These cleaning solutions
include Kodak lens cleaner, Windex, and Sparkle. Some people recommend
cutting the concentration of these solutions with distilled water
(50%). Sparkle seems to have somewhat of a cult following-it is alcohol
and ammonia free-and it is what we have been using here for some time. In
all cases, care must be taken to drag the contamination and grit away from
the lens using lens tissue. Work from the inside of the lens out, and make
sure not to smear contamination across areas which you have just cleaned
(use the lens tissue to trap the contamination, not just move it
around). The aqueous detergent methods seem to be safe for lens
coatings. Oil objectives do not need to be cleaned with detergent each
use-wiping the oil off with lens tissue should suffice as long as different
formulations of immersion oil are not being used on the same
objectives. Apparently, immersion oils from different sources can react to
produce a sort of sludge which is then more difficult to remove. Also,
old-fashioned natural immersion oils such as pine oil have a volatile
component which will evaporate and leave a gummy residue behind. Inverted
microscopes may benefit from the use of little "diapers" for the objective
lenses which absorb excess oil and prevent it from leaking back into the
objective. Preventative maintenence is the goal here.
The use of organic solvents is not for the faint of heart, however
some brave souls manage to use these methods without incident. The less
fortunate tell tales of leaking lenses (a problem particularly on inverted
scopes) and even lens components dropping out of the barrel of the
objective. Attention to proper technique is important. High quality
cotton tipped applicators are favored for cleaning objectives with strong
solvents-some people make their own and some people purchase them. A
common theme is to begin at the center of the lens and work outward, slowly
rotating the cotton swab so as to pick up the contamination rather than
drag it about the lens. Using a dry applicator or reusing any portion of
the applicator can scratch the lens. The cement which holds the lens
together is easily dissolved using some of these solvents, and so great
care must be taken not to "drown" the lens in solvent. The applicator
should be neither dry nor saturated with solvent. It is important not to
touch the cotton and contaminate it with oil from one's fingers. In an
alternative method, one gently and unidirectionally drags a single-use
strip of lens paper dampened in one spot with the solvent across the
lens. Favored solvents include: lighter fluid (apparently used by some
service reps), anhydrous ether, a mixture of diethylether and benzene,
spectroscopy grade chloroform, isopropanol, methanol and xylene. There are
others, to be sure. A common final touch is to fog the lens using
distilled water from one's breath, and polish with one unidirectional swipe
of a high quality lens paper.
I hope this summary helps those of you who have expressed interest
in the subject of objective cleaning and once again I thank everyone for
thier input.
Best Regards,
Karl G.

At 07:17 PM 9/17/2002 -0500, Dwight Arrieche (IIBCA)" (by way wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Wed Sep 18 11:19:01 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Wed, 18 Sep 2002 09:12:10 -0700
Subject: Re: LM lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You could try a 50:50 mix of Windex (or any clear window-cleaning fluid) and
distilled water. Use Kimwipes or cotton buds or cloth, never Kleenex, with
many gentle wipes rather than a few vigorous ones, and be careful to dry the
lens at the end. Hope this helps.

Lesley Weston.



on 17/09/2002 5:17 PM, Dwight Arrieche (IIBCA) (by way of
MicroscopyListserver) at darriech-at-cumana.sucre.udo.edu.ve wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear List Servers
}
} I need some help for cleaning or removing oil from LM lenses.
} What is the recommended method and solvents for this task?
}
} Thanks
}
} D. Arrieche
} IIBCA-UDO
} darriech-at-cumana.sucre.udo.edu.ve
}



From daemon Wed Sep 18 12:21:23 2002



From: Tom Moninger :      thomas-moninger-at-uiowa.edu
Date: Wed, 18 Sep 2002 12:12:42 -0500
Subject: Whole lung

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone experience with embedding whole human lung in paraffin or
methacrylate, especially if the lung was previously air-dried while inflated?
Thanks, Tom

Thomas Moninger (thomas-moninger-at-uiowa.edu)
University of Iowa Central Microscopy Research Facility (www.uiowa.edu/~cemrf)
View expressed are my own.



From daemon Wed Sep 18 12:36:31 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 18 Sep 2002 13:27:15 -0400
Subject: TEM automated sample prep tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard,
I would like to remind you that there will be a TEM symposium at next year's M&M meeting. If you want to write it up, that may be the perfect place.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Richard Beanland [mailto:richard.beanland-at-bookham.com]
Sent: Wednesday, September 18, 2002 8:16 AM
To: 'jerzy.gazda-at-amd.com'
Cc: Microscopy-at-sparc5.microscopy.com


Hi Jerzy,
we have a similar workload to you, but in III-V's (we have looked at
475 TEM samples since April 1). We have only 2 people (myself and one
other) doing all the sample prep, TEM and report writing. Much of our work
is straightforward thickness measurements and cross-sections, and we have no
FIB.

Do you have a Gatan PIPS or similar low-angle ion mill, with the ability
to mill from predominantly one direction? I've found a great way of making
cross sections using this machine combined with precision(ish) cleaving,
cutting out several stages from the 'standard' way of doing things. Typical
InP cross sections take less than an hour to do; with the right kind of
sample (i.e. not site specific in 2 dimensions) it is possible to stack them
up and do up to 10 at a time. It's straightforward to hit features down to
20um in size, but if you want to keep the specimen you really have to do one
at a time. (It's impossible to get several specific sites thin at the same
time, meaning you have to look at the first region that becomes electron
transparent, then destroy it while getting the next one thin, etc..) Si
takes longer since it ion mills so much more slowly, but the few I've done
still take less than 90 mins from getting the sample to looking at it in the
TEM.

I've been thinking of writing this up and publishing it somewhere, since
it has saved us a huge amount of time and effort. I'm sure that if you were
working with Si all the time you'd be able to optimise the procedure still
further. I'll try to get some words together describing the procedure, with
pictures, if you like.

FIB is great for very specific sites (less than 10um or so, depending on the
structure) and I do use one in a tame academic's lab every so often, but for
fast cross sections with lots of thin area I can't beat 'standard' methods.
However I appreciate that Si geometries are often several orders of
magnitude smaller and more complex than III-Vs, and site specific samples
are likely to be a much bigger proportion of your workload.

Good luck,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com




-----Original Message-----
} From: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Sent: 17 September 2002 23:09
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample
preparation in our TEM laboratory and would like to hear your opinions on
the following semi-automated approaches. The lab processes nearly a 1000 TEM
samples per year, mostly cross-sections of CMOS devices on Si wafers. We use
manual polishing, dimpling, ion mills, and FIBs tools, however we don't have
enough people to handle the load and will not see an increase in headcount
in the near future. I would appreciate any and all input on the following
combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with
those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White
Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





=======================================================================
This e-mail is intended for the person it is addressed to only. The
information contained in it may be confidential and/or protected by
law. If you are not the intended recipient of this message, you must
not make any use of this information, or copy or show it to any
person. Please contact us immediately to tell us that you have
received this e-mail, and return the original to us. Any use,
forwarding, printing or copying of this message is strictly prohibited.

No part of this message can be considered a request for goods or
services.
=======================================================================
Any questions about Bookham's E-Mail service should be directed to postmaster-at-bookham.com.



From daemon Wed Sep 18 12:39:10 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 18 Sep 2002 13:32:39 -0400
Subject: TEM automated sample prep tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am curious. If you have an FIB, why don't you set up several sample areas to be milled overnight, come in a clean them up, and then use the pluck-out system? If you have multiple samples, you could cut them down or cleave them and mount them on a holder and then use the auto milling program.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Sent: Tuesday, September 17, 2002 6:09 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample preparation in our TEM laboratory and would like to hear your opinions on the following semi-automated approaches. The lab processes nearly a 1000 TEM samples per year, mostly cross-sections of CMOS devices on Si wafers. We use manual polishing, dimpling, ion mills, and FIBs tools, however we don't have enough people to handle the load and will not see an increase in headcount in the near future. I would appreciate any and all input on the following combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From daemon Wed Sep 18 13:03:38 2002



From: Jiang Liu :      jiangliu24680-at-hotmail.com
Date: Wed, 18 Sep 2002 13:56:07 -0400
Subject: AFM phase imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:

I have been working on phase imaging of high impact polystyrene (HIPS), a
composite of polystyrene and polybutadiene rubber particles, with DI AFM
TappingMode. I have tried different microtoming conditions to optimize the
cutting and therefore the imaging from those specimens, but with limited
success. I have also found cutting temperature (tried from 0 C to -80 C) is
very important but same temperature results in different cutting qualities,
primarily chatters and local compressions on polystyrene matrix, from
different specimens. However, -40 C is good for any specimens of impact
copolymer of propylene and ethylene.

Does anybody have any experiences, suggestions or comments? Many thanks in
advance.

Jiang Liu, PhD
Research and Technology Center
ATOFINA Petrochemicals
(281) 884-0529
Jiang.liu-at-atofina.com


_________________________________________________________________
Chat with friends online, try MSN Messenger: http://messenger.msn.com



From daemon Wed Sep 18 13:38:26 2002



From: Frida.Maiers-at-co.hennepin.mn.us
Date: Wed, 18 Sep 2002 13:29:59 -0500
Subject: gloves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Working in a clinical lab of a hospital we are under pressure to eliminate
latex gloves in favor of nitrile and now vinyl. Does anyone have
appropriate references to support any of these, none of these or some of
these? Thanks in advance for your help.
F. Maiers




From daemon Wed Sep 18 15:05:16 2002



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Wed, 18 Sep 2002 15:56:40 -0400
Subject: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sharon,
We have been using an EPSON Expression 1600.
The film holders did not come in 3.5x4 but I use the holder for four
- 4x5s and with 3 sides supported, all is well.
The machine works well, the limitation is my lack of knowledge in
working in Adobe to make the images look their best.
Pat Connelly
Univ. of Pennsylvania, Biology Dept.


From daemon Wed Sep 18 15:12:34 2002



From: JHoffpa464-at-aol.com
Date: Wed, 18 Sep 2002 16:05:47 -0400
Subject: Re: Philips EM 201 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


why an independant service. fei does a great job.
john


From daemon Wed Sep 18 15:39:36 2002



From: robert.fowler-at-tdktca.com
Date: Wed, 18 Sep 2002 16:36:37 -0400
Subject: Re: book search / plating anomalies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



You may want to try "The Electrodeposition Of Tin And Its Alloys" by Dr
Manfred Jordan ISBN 3-87480-118-7 This is an excellent reference with lots
of SEM pictures.

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



"Oakley, Jeff"
{oakleyj-at-rayov To: "MSA listserver"
ac.com} {Microscopy-at-sparc5.microscopy.com}
cc:
09/18/2002 Subject: book search / plating anomalies
11:37 AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am looking for a reference that documents various anomalies associated
with electroplating and electroless plating of metals such as gold, copper,
nickel, zinc, etc., as seen through the SEM.

I took a tour through Amazon's bookshelves but didn't see anything that
matched what I was looking for.

TIA,

Jeff Oakley






From daemon Wed Sep 18 15:57:53 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 18 Sep 2002 16:45:53 +0200
Subject: Re: Philips EM 201 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,

by asking for an independent service I did not want to imply that I ever
was dissatisfied with the Fei/Philips service. In my lab in Germany we
have a CM 10 which is serviced by Fei/Philips for over 10 years and they
always did a great job and sometimes even more than that!
Looking for an independent service for the 201 has more to do with the
age of the instrument. I don´t know if Fei services instruments that
old, but was in the process of contacting them and get this information.

Stefan





From daemon Wed Sep 18 16:21:09 2002



From: evgenia.pekarskaya-at-exxonmobil.com
Date: Wed, 18 Sep 2002 17:13:46 -0400
Subject: Postdoc position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Post doctoral position available at the University of New Orleans/ Advanced
Materials Research Institute (AMRI)

Requirements : Background in Materials Sciences and experience in the
operation of a TEM

Project :
Li-ion batteries are used as power source in portable electronics and
medical devices. After high cycle number the capacity of these batteries
decreases which is partly due to structural instabilities of the cathode
(LiCoO2). The study the crystal structure and lattice defects in LiCoO2
before and after electrochemical cycling is aimed at a better understanding
of the failure mechanisms leading to the reduced performance.

Contact :
Heike Gabrisch
Assistant Professor of Chemistry
Department of Chemistry/AMRI
University of New Orleans
New Orleans, LA 70148

hgabrisc-at-uno.edu... phone (504 )280-1122 ... fax (504) 280-3185 ...


**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355




From daemon Wed Sep 18 16:49:03 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Wed, 18 Sep 2002 14:32:49 -0700
Subject: Re: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian:

I have a recipe I can share from Bernie Kestel which was used on our
Model
550 Jet Polisher. I'm sure it won't translate exactly to your
equipment,
but it may be a good starting point. I hope it helps.


Material: Ni-10Co

Electrolyte: 10% HClO4
90% ethanol

Temperature: 0 degrees C

Jet Height: 3.9mm

Pump Speed: 2-4

Volts: 20

Current: 35 mA

Comments: After termination, rinse specimen immediately.

This is from an Argonne National Lab Publication (ANL-80-120) titled
"Polishing Methods for Metallic and Ceramic Transmission Electron
Micrscopy
Specimens" by Bernie Kestel.

I don't know if it is available from Argonne anymore, but I can supply
you
with a copy of the booklet (about 60 pages) if you think it would be
useful. It discusses many different jet polishing techniques although
the
majority of it is done with one of our Model 550 Jet Polishers.

Best regards-

David

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


Ian MacLaren wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear all,
} Does anyone have any good electropolishing recipes for Co-Ni alloys? We
} have a Struers Tenupol. Suggestions for voltage and temperature would
} be good too.
}
} Thanks in advance
}
} --
} Ian MacLaren
} Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
} ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/


From daemon Wed Sep 18 16:52:57 2002



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Wed, 18 Sep 2002 16:33:57 -0500
Subject: Re: LM lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Dwight,
I compiled answers to your question from a previous inquiry a year
or so ago.
Let me know if it helps. I have pasted it in at the end of this
message.
Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242-1324

At 07:17 PM 9/17/2002 -0500, you wrote:
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


BEST SOLVENT FOR REMOVING IMMERSION OIL FROM OBJECTIVE LENS

I asked if xylene was the best solvent for cleaning immersion oil off a
non-immersion objective. Here is the array of responses I received. The
diversity may interest you.

1. We regularly use xylene with a cotton tipped applicator to clean our
lenses and remove immersion oil. This was recommended to us by the
supplier of our lenses and microscopes for a number of reasons. The
primary reason is that xylene effectively 'cuts' the oil, without damaging
the lens. Acetone will affect the lens coating, and isopropyl alcohol only
rinses the oil without effectively removing it.

2. Use alcohol on lens paper. Never use xylene or toluene; these solvents
can dissolve the cement used to seat the various lens elements.

3. I would recommend Sparkle Glass Cleaner. I only use solvents as a very
last resort. The cements are soluble in most solvents. With intractable
grime, I use a cotton tipped applicator moistened with either xylenes or
toluene and shake all excess off. The tip must be moist, not wet or dry,
and make single passes until the grime come off. Use only enough solvent
on the applicator to dampen the surface, not enough to wet.

4. For day-to-day cleaning of lenses, including oil on the 40X, invert an
ocular and examine the surface for cleanliness. If it is clean, don't
clean it. Dust off any loose debris. Check the lens again. Moisten a
cotton tipped applicator in Sparkle and wipe the lens once with a rolling
action to present a new surface and lift off any grit. Discard. Take a
dry applicator and remove the film. Check the lens with an inverted ocular
to see if it is clean. Do no more than is necessary.

5. The care instructions for my immersion oil suggest cleaning with a soft
cloth or lens tissue (no Kimwipes) moistened with ether/alcohol (7:3) or
xylenes. My microscope manuals recommend removing fingerprints using alcohol

6. We use 70 % isopropanol to clean immersion oil off of our lenses.

7. Cleaning with xylene is a little drastic. If the lens eventually gets
xylene in behind the cement it could do some damage and you would have to
send it in for repair. I use Kodak lens cleaner and some lens cleaning
tissues (not Kimwipes) to get the oil off, constantly checking under a
dissecting scope to make sure that it is all removed. This works well on
some expensive lens that we have here.

8. We use Green Soap from the pharmacy. It works best with ultra pure water.

9. Alcohol is usually safer than xylene, but safer still is diluted
detergent, such as "Joy" or similar, followed by water. Use alcohol only
if that doesn't work. Fumes from xylene, toluene, etc. are best avoided.

10. Back in the old days, toluene was a recommended solvent for cleaning
lenses of immersion oil. These days we wipe off excess oil with lens
tissue and then clean the lens with Kodak lens cleaner solution.

11. The best way to clean immersion oil from a lens is to use lighter
fluid. First remove as much oil from the lens with lens paper. Be gentle;
don't rub. Then dip a cotton swab in the lighter fluid and lightly wipe it
across the lens. All the oil will be removed and it will evaporate very
quickly without leaving a residue or streaks. Xylene is not recommended as
it can dissolve the adhesives holding the lens in place.



From daemon Wed Sep 18 17:48:17 2002



From: Greg Mulhollan :      mulhollan-at-extremedevices.com
Date: Wed, 18 Sep 2002 17:40:21 -0500
Subject: Commercial Cathodoluminescence Lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Microscopy Folks,
I am trying to find a commercial or perhaps university lab which will
allow us to so some SEM cathodoluminescence measurements on carbon
field emitters. Any pointers? Thanks,
Greg M.


Gregory Mulhollan, Ph.D.
Senior Scientist
Extreme Devices Inc.
3500 ComSouth Drive
Austin, TX 78744
(512)439-3512 voice
(512)439-3487 fax
mulhollan-at-extremedevices.com



From daemon Wed Sep 18 18:25:49 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 18 Sep 2002 18:18:52 -0500
Subject: back issues of JCB for the asking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopists,
I have a run of Journal of Cell Biology from 1987 through
2001 that I would like to give away. Our library doesn't want them.
Anyone out there interested? Or know of a connection to libraries
somewhere that might be interested?

Thanks,
Tobias Baskin
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Wed Sep 18 23:18:03 2002



From: Donald Werder :      aettinger1984-at-comcast.net
Date: Thu, 19 Sep 2002 00:02:40 -0400
Subject: Re: book search / plating anomalies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeff,
I only have experience in imaging electroless copper by TEM. TEM shows
small bubbles in the metal. I don't remember the details as this was
some time ago. I believe they are produced by Hydrogen, which tends to
make the metals more brittle.

Don Werder

Oakley, Jeff wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Sep 19 06:55:37 2002



From: Oparowski, Joseph :      Joseph_Oparowski-at-bose.com
Date: Thu, 19 Sep 2002 07:47:54 -0400
Subject: book search / plating anomalies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeff,

You may want to check publications from ASM international
(www.asminternational.org). Start with Volume 5 (10th edition) of the
Metals Handbooks-Surface Engineering. Volume 11 deals with Failure Analysis
and Prevention and may have some examples. Good Luck.


Joseph

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313


-----Original Message-----
} From: Oakley, Jeff [mailto:oakleyj-at-rayovac.com]
Sent: Wednesday, September 18, 2002 11:38 AM
To: MSA listserver


I am looking for a reference that documents various anomalies associated
with electroplating and electroless plating of metals such as gold, copper,
nickel, zinc, etc., as seen through the SEM.

I took a tour through Amazon's bookshelves but didn't see anything that
matched what I was looking for.

TIA,

Jeff Oakley


From daemon Thu Sep 19 07:53:12 2002



From: robert.fowler-at-tdktca.com
Date: Thu, 19 Sep 2002 13:05:59 -0400
Subject: hf antidote gel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

You might want to contact Dr. Fred Hossler at hossler-at-ETSU.Edu. He is very
helpful in these matters.

JD Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Tom Moninger" {thomas-moninger-at-uiowa.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, September 18, 2002 1:12 PM



Hi Folks I am curious if any of you using HF are utilizing the hf antidote
gel. If you are where can I find some in US. Thank You

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com


THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY
TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL
INFORMATION.

If you are not the intended recipient, be advised that any use,
dissemination, distribution or duplication of this transmission is strictly
prohibited. If you received this transmission in error, please notify the
sender immediately by electronic reply to this transmission or by phone
(847-803-6100). Thank you.




From daemon Thu Sep 19 12:11:17 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 19 Sep 2002 12:01:47 -0500
Subject: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
We, being non-material people, need some help with a material sample.
The sample is a high impact polystyrene (HIPS) that contains butadiene
rubber moieties. We need to be able to identify the location of these
rubber moieties and understand that they will stain upon exposure to osmium.
Does anyone have any experience with this type of sample. Would it be
advisable to stain by floating thin sections (on grids) on liquid osmium or
by using osmium vapors? What type of grids would be most desirable to
minimize corrosion by the osmium?

Also, we have a diamond knife made by Diatech in Tennessee. I need to
contact them to obtain information about this knife. Does anyone have a
phone number or other contact information?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Thu Sep 19 12:12:51 2002



From: Mick Thomas :      mgt3-at-ccmr.cornell.edu
Date: Thu, 19 Sep 2002 13:08:29 -0400
Subject: SF6 safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists,

I would be very grateful if someone could offer me advice on how to ensure
that a microscopy room is set up to safely handle a leak of SF6.

Thank you very much for your time and help.

Sincerely,

Mick

-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu



From daemon Thu Sep 19 13:38:07 2002



From: Kai Lorcharoensery :      kai-at-lehigh.edu
Date: Thu, 19 Sep 2002 14:35:47 -0400
Subject: Re: hf antidote gel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The gel is calcium gluconate. You can buy a few tubes from Fisher (HF
antidote gel) or Pharmascience.

Kai Lorcharoensery
Materials Science & Engineering
Lehigh University
5 E. Packer Ave.
Bethlehem, PA 18015

"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com wrote:

} Hi Folks I am curious if any of you using HF are utilizing the hf antidote gel. If you are where can I find some in US. Thank You
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}



From daemon Thu Sep 19 14:17:05 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 19 Sep 2002 15:15:47 -0400
Subject: Commercial Cathodoluminescence Lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Greg;

One of our folks in my dept. worked at Ohio State in the group that used
cathodeluminescence.

The contact he gave me at Ohio State is below.

Regards,

Peter Tomic
Anadigics, Inc.

Professor Len Brillson
Department of Electrical Engineering



-----Original Message-----
} From: Greg Mulhollan [mailto:mulhollan-at-extremedevices.com]
Sent: Wednesday, September 18, 2002 6:40 PM
To: Microscopy-at-sparc5.microscopy.com


Hi Microscopy Folks,
I am trying to find a commercial or perhaps university lab which will
allow us to so some SEM cathodoluminescence measurements on carbon
field emitters. Any pointers? Thanks,
Greg M.


Gregory Mulhollan, Ph.D.
Senior Scientist
Extreme Devices Inc.
3500 ComSouth Drive
Austin, TX 78744
(512)439-3512 voice
(512)439-3487 fax
mulhollan-at-extremedevices.com



From daemon Thu Sep 19 14:17:42 2002



From: Nancy Cherim :      nac-at-cisunix.unh.edu
Date: Thu, 19 Sep 2002 15:15:18 -0400
Subject: Hitachi H600 available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Hello listers,
The University of New Hamphsire has a Hitachi H600 100 kV STEM with an
H-6010 scanning system (working), an Oxford thin window EDS (working) and a
PGT IMIX-PC system that we are in the process of replacing. The microscope
was installed in 1986 and is not operational due to a high voltage buffer
problem (we think). Also, the PGT Sun system needs a new hard drive (we
think). The microscope has 2 sets of 30 plate film cassettes and boxes with
vacuum film desiccator. We will be dismantling the scope soon. Interested
parties looking for microscope parts, or interested in getting this one to
work, please contact me with inquiries/offers off-line. It will be the
donee's responsibility to come and take the microscope or pay removal,
shipping and handling charges. I am open to discuss any offers.
I will also post details on Nestor's surplus equipment list at the MSA
website.

Thanks!


Dr. Jeff Simpson
Electron Microscope Facility
University Instrumentation Center
University of New Hampshire
Kendall Hall
Durham, NH 03824
603-862-2457
jeff.simpson-at-unh.edu

Visit us at http://www.unh.edu/instrumentation-center/about.html



From daemon Thu Sep 19 15:03:38 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 19 Sep 2002 14:55:37 -0500
Subject: SEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I am looking for a very fine grained film to use for capture of SEM
images. We want to use this film in place of the Polaroid Type 55P/N film.
Kodak recommends the Tmax 100 4x5 sheet film.
I would appreciate other recommendations or confirmation that this would
be the film of choice.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Thu Sep 19 15:24:22 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 19 Sep 2002 15:16:00 -0500
Subject: Re: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debbie,
There is some literature suggesting that ruthenium tetroxide
is better than osmium for this kind of thing. There is a paper by
Trent, Scheinbeim, and Couchaman 1983 Macromols 16: 589-598 that is
very informative. My understanding is that it is usual to vapor
stain this kind of sample.
Hope this helps,
Tobias
}
}
}
} Listers,
} We, being non-material people, need some help with a material sample.
} The sample is a high impact polystyrene (HIPS) that contains butadiene
} rubber moieties. We need to be able to identify the location of these
} rubber moieties and understand that they will stain upon exposure to osmium.
} Does anyone have any experience with this type of sample. Would it be
} advisable to stain by floating thin sections (on grids) on liquid osmium or
} by using osmium vapors? What type of grids would be most desirable to
} minimize corrosion by the osmium?
}
} Also, we have a diamond knife made by Diatech in Tennessee. I need to
} contact them to obtain information about this knife. Does anyone have a
} phone number or other contact information?
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Thu Sep 19 16:21:26 2002



From: Gillies,Concettina :      Gillies-at-up.uchc.edu
Date: Thu, 19 Sep 2002 17:13:27 -0400
Subject: EM Technologist position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Opening for an experienced EM tech. at the Univ. of Connecticut Health
Center.
Prefer candidate with clinical experience.
Details available online at http://employ.uchc.edu/


From daemon Thu Sep 19 16:55:12 2002



From: Tom Murray :      murraytm-at-u.washington.edu
Date: Thu, 19 Sep 2002 14:48:38 -0700
Subject: Philips 300 HT Tank

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Sorry if this is a duplicate, but the first time didn't seem to go through.

I have a HT tank for a Philips 300 which I have no use for. The part number
is 4022 185 00651. It is already crated. I do not know if it is in working
order. The crate has a RMA number on it, but I don't know it was fixed and
returned or if it was never sent in for repairs.

Available to anyone who will cover shipping.

If I don't hear from any interested parties by the end of next week, I will
pursue scrapping it.

Tom
--
Thomas M. Murray Phone: (206)543-2836
Electron Microscopy Center Manager Fax: (206)543-3100
Materials Science & Engineering email: murraytm-at-u.washington.edu
Roberts Hall 130
University of Washington
Seattle, WA 98195



From daemon Thu Sep 19 18:07:22 2002



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 19 Sep 2002 15:55:55 -0700
Subject: Re: SEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debby,

I would certainly consider Kodak Tech Pan 4415. Speed is dependent on
development but will be slower than T-Max, around 25-32 and grain structure
is very fine.

Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
http://katie.ucdavis.edu
raharris-at-ucdavis.edu




} Listers,
} I am looking for a very fine grained film to use for capture of SEM
} images. We want to use this film in place of the Polaroid Type 55P/N film.
} Kodak recommends the Tmax 100 4x5 sheet film.
} I would appreciate other recommendations or confirmation that this would
} be the film of choice.
}
} Thanks,
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907



From daemon Thu Sep 19 19:21:18 2002



From: HWHAMILT-at-UTMB.EDU (by way of Ask-A-Microscopist)
Date: Thu, 19 Sep 2002 19:07:22 -0500
Subject: Ask-A-Microscopist: Hobby LM Purchase

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (HWHAMILT-at-UTMB.EDU) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
September 19, 2002 at 15:24:08
---------------------------------------------------------------------------

Email: HWHAMILT-at-UTMB.EDU
Name: Dub Hamilton

Organization: Utmb Galveston

Education: Graduate College

Location: galveston Texas

Question: I am interested in purchasing a microscope for 'serious
hobby use'. A number are available at about $500 with a 1.25
adjustable abbe condensers.(looking at Accu-scopy a3025 and LW
Scientific Observer IV) Questions:
1. Is this about right for me?
2. Is Kohler illumination possible with this condenser or is
something eles required?
3.Is achromatic objectives ok for my purpose?

thanks

---------------------------------------------------------------------------


From daemon Thu Sep 19 21:27:22 2002



From: Ian Kaplin :      ian-at-mail.emu.usyd.edu.au
Date: Fri, 20 Sep 2002 12:16:24 +1000
Subject: Os staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The method we use is to stain in Os vapour for 30 min to an hour on
ultrathin sections. We normally use copper grids without any problems.

Put a couple of drops of 2% stock Os solution in a jar, position the
grids above the liquid (we use an old flexible plastic grid holder)
and leave capped in fumehood for the time.

Leave the jar open to air out after removal of Os at least overnight
before discarding the jar and leave the grids open to air for awhile
before putting in microscope to get rid of Os smell.

--

Ian Kaplin

Electron Microscope Unit
Madsen Bldg F09
The University of Sydney
NSW 2006
Australia

Tel : 61 2 9351 3302
Fax : 61 2 9351 7682


From daemon Thu Sep 19 23:52:43 2002



From: sstouden-at-thelinks.com
Date: Thu, 19 Sep 2002 23:40:17 -0500 (CDT)
Subject: Re: Software: speech recognition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


there is a product called Reachout which was designed forthis.
I used it for a person totally disabled back in 1998.. he had an eyeball
controlled keyboard and could use windows nt very well with it from his
always "on the bak possition. he was paralyzed from his neck down.21
years old at time.
you might search fo it I believe the people that made it were in Florida.
if you need more help let me know.

sterling

On Tue, 17 Sep 2002, John J. Bozzola wrote:

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} I apologize is this is not strictly a microscopy question. A
} microscopist colleague/friend of mine is interested in purchasing a
} high quality speech recognition software. The individual is
} handicapped and does not full use of their hands and would be using
} the software for scientific writing (primarily describing microscopy
} findings). Although I favor the Macintosh system (since they would
} also use the computer for image processing and publishing), I am
} looking for the best software to assist this individual. Any
} recommendations would be appreciated. Many thanks.
}
} JB
}
} ##############################################################
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} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
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} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}



From daemon Fri Sep 20 01:38:45 2002



From: =?ISO-8859-2?Q?Old=F8ich_Benada?= :      benada-at-biomed.cas.cz
Date: Fri, 20 Sep 2002 08:30:15 +0200
Subject: Heavily contaminated C2 apertures

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Dear colleagues,
C2 apertures in our CM12 are heavily contaminated. Does anybody could suggest how
to clean them? Heating in molybdenum boat does not work.
Thanks for any responses. O. Benada



+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-41062399
Fax: +420-2-41062347
WEB: http://www.biomed.cas.cz/mbu/lem113/lem.htm



From daemon Fri Sep 20 02:56:55 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 20 Sep 2002 08:49:05 +0100
Subject: Re: SEM film

Contents Retrieved from Microscopy Listserver Archives
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Debby
I have used Kodak TMax 100 in 35mm and 120 (6x7cm) formats for many
years and can thoroughly recommend it.
In 6x7 cm images the film can record all of the data available from
the SEM record monitor. Images are suitable for enlargement to at
least 16x20", often 20x30" and at these mags film grain is much less
important than image noise. I doubt if you would gain very much by
using 5"x4", and the negatives would be much more trouble to process.
However, there is significant loss of image quality when 35mm format
is used. This may be unimportant for images of the size required in
scientific publications, but degrades the quality of exhibition
images.
Chris

} Debby,
}
} I would certainly consider Kodak Tech Pan 4415. Speed is dependent
on
} development but will be slower than T-Max, around 25-32 and grain
structure
} is very fine.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu
}
}
}
}
} } Listers,
} } I am looking for a very fine grained film to use for capture of
SEM
} } images. We want to use this film in place of the Polaroid Type
55P/N film.
} } Kodak recommends the Tmax 100 4x5 sheet film.
} } I would appreciate other recommendations or confirmation that
this would
} } be the film of choice.
} }
} } Thanks,
} } Debby
} }
} } Debby Sherman, Manager Phone: 765-494-6666
} } Life Science Microscopy Facility FAX: 765-494-5896
} } Purdue University E-mail: dsherman-at-purdue.edu
} } S-052 Whistler Building
} } West Lafayette, IN 47907
}
}



From daemon Fri Sep 20 04:05:13 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Fri, 20 Sep 2002 10:57:06 +0200
Subject: TEM heating/deformation stage

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
We are interested in purchasing a heating/tensile stage for our Phillips
CM20 (Supertwin lens). We are intending to use this for studying
deformation in thin films.

I would appreciate information and approximate prices from manufacturers.

I would also appreciate comments from users of such stages.

Thanks in advance

--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Fri Sep 20 05:24:19 2002



From: Dr Adam Papworth :      ajp5-at-liverpool.ac.uk
Date: Fri, 20 Sep 2002 11:21:53 +0100
Subject: VG HB601 UX problems

Contents Retrieved from Microscopy Listserver Archives
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Dear All VG Users,

Here at Liverpool we have a VG HB 601 UX, which has developed a major
fault within the way the probe is scanned. However, before the scanning
fault developed, there were other intermittent faults and I feel that
all these faults are connected.
The first intermittent fault was an error message informing;

18:09:42 L9
Column Crate Interface
Reset
followed by
18:09:42 L9
Column Crate Interface
18:09:42 L9
Column Crate Interface
+15V Failure
This failure resulted in the stage translates not working.
By rebooting both computers the fault would be cleared, this fault would
happen once a week and then it progressed to once a day.

After rebooting sometimes the scanning would not be correct, i.e.
instead of an image appearing we would just get parallel lines or the
image of the VOA would be twisted and distorted.
The faults have now progressed to a point of not being able to form an
image at all in image mode, in Lo Mag Mode the image sometimes forms,
however we can see it flick on and off without touching the console. In
diffraction mode, sometimes we can see the objective aperture but
usually no image of the ADF detector
We have now lost all power to the middle and right hand side of the
control panel and the console will not boot up anymore.

All help and ideas on where to start in rectifying these faults would be
greatly welcome.

Adam

Please could all replies be to my e-mail address: ajp5-at-liv.ac.uk


--
Dr Adam Papworth,
Senior Experimental Officer,
Department of Engineering,
The University of Liverpool,
Liverpool,
L69 3GH, UK.

Phone
(Work) 0151 794 4672
(Mobile) 0151 794 7587
07970 24 7587
(Home) 0151 283 8596
(FAX) 0151 794 4675
e-mail ajp5-at-liv.ac.uk




From daemon Fri Sep 20 06:54:04 2002



From: John Peters :      edwhard465tom-at-estpak.ee
Date: Fri, 20 Sep 2002 04:44:22 -1900
Subject: Did You Get It yet 7950

Contents Retrieved from Microscopy Listserver Archives
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From daemon Fri Sep 20 07:22:16 2002



From: robert.fowler-at-tdktca.com
Date: Fri, 20 Sep 2002 08:19:35 -0400
Subject: Re: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Maybe this is the location you want?
















Dia-Tech Corporation
6408 Clinton Highway, Powell, TN 37849
(865) 938-2720









Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



Debby Sherman
{dsherman-at-purd To: "message to: MSA list"
ue.edu} {microscopy-at-sparc5.microscopy.com}
cc:
09/19/2002 Subject: OS staining of rubber
01:01 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
We, being non-material people, need some help with a material sample.
The sample is a high impact polystyrene (HIPS) that contains butadiene
rubber moieties. We need to be able to identify the location of these
rubber moieties and understand that they will stain upon exposure to
osmium.
Does anyone have any experience with this type of sample. Would it be
advisable to stain by floating thin sections (on grids) on liquid osmium or
by using osmium vapors? What type of grids would be most desirable to
minimize corrosion by the osmium?

Also, we have a diamond knife made by Diatech in Tennessee. I need to
contact them to obtain information about this knife. Does anyone have a
phone number or other contact information?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907







From daemon Fri Sep 20 07:35:02 2002



From: zaluzec-at-microscopy.com
Date: Fri, 20 Sep 2002 07:24:34 -0500
Subject: Re: Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Plasma Cleaning is another solution which I have used
on Condensor Apertures. Make sure you use the most
reactive species possible... i.e. an Oxygen Rich Plasma.

If they are badly contaminated you may have to go
to the high power Plasma Ashing regime.

However, if they are very heavily contaminated even that may
not work since some residue may remain. In this case you will
just have to replace them.


Disclaimer: My Employer ANL holds the Patent on Plasma Cleaning
Technology for application to Electron Microscopy Specimens &
In-situ Microscope applications. There are also commerical
organizations which may be able to help you such as SPI, XEI, South
Bay Tech and Fischione, all of whom are licensee's of the ANL Patent.
Links to their respective WWW pages are on the WWW page below.

http://www.amc.anl.gov/Docs/ANL/TechTrans/PlasmaCleaning.html



Cheers...

Nestor
Your Friendly Neighborhood SysOp



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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Sep 20 07:47:23 2002



From: zaluzec-at-microscopy.com
Date: Fri, 20 Sep 2002 07:32:34 -0500
Subject: Re: VG HB601 UX problems

Contents Retrieved from Microscopy Listserver Archives
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Adam

Check the 10/20 volt power supplies in the electronics racks. The
symptoms you describe I have seen many times.

Look both at the fuses as well as the thermal sensors on each
of the power supplies. Both of which are easily checked by measuring
the resistance with an Ohm meter. Both of which have failed on my system
at various times. I would tend to suspect the thermal sensor.

These units are located on my system (603Z) on the back of the Electronic
Power Supply Rack, underneath the Objective Power Supply, on the
601 they might be elsewhere.

You can tell immediately if you have power to these units by looking for the
"red" LED's on each of the units. They should be lit. If off, then power down
the machine and pull the suspect unit and start checking each one. Start
with the higher voltage supplies (20V) since they also supply power to a few
of the lower voltage (10V) supplies.

Cheers....

Nestor
Your Friendly Neighborhood SysOp.






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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Sep 20 07:59:43 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 20 Sep 2002 08:52:11 -0400
Subject: Re: SEM film

Contents Retrieved from Microscopy Listserver Archives
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I have always felt that Polaroid P/N 55 was a very good choice for SEM
image capture. While the positive print is nothing to write home about,
the negative is of very high quality (in terms of sharpness, line to line
resolution, and so on - granted it is rather low contrast). When printed
on the old grade 4 Ilfobrom paper it gave excellent prints. I am assuming
you are using fresh film, of course. A number of years ago I experimented
with switching from Type 55 to conventional film, but my users wouldn't use
it, and in the end I gave up on it myself. The fiddle of loading film
holders and developing and printing wasn't worth the cost saving to most
users. In the meantime they had working copies of all their images in the
Polaroid positives. Not only that, but the conventional process required
MUCH more support from me and my staff (how many of today's students knows
the first thing about conventional B&W photography?)

The resolution and graininess of large format films for SEM images is
usually quite unimportant, because the limiting factor in the image quality
is the screen of the CRT. Even a relatively coarse-grain film like the old
Kodak Tri-X can resolve better than 50 lines/mm (fine-grain films can
resolve over 300 lines/mm). To generate this amount of information, on a
10cm^2 image, would require 10,000x10,000 pixels. I don't know of a CRT
that can resolve that!

Of course, other labs' priorities may not be that same as mine, and there
may be excellent reasons to switch from Polaroid, but my choice was to not
follow that route.

Tony.

At 02:55 PM 9/19/2002 -0500, Debby Sherman wrote:
} ------------------------------------------------------------------------
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** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Fri Sep 20 08:13:16 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 20 Sep 2002 09:12:24 -0400
Subject: Re: hf antidote gel

Contents Retrieved from Microscopy Listserver Archives
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Kai;

We routinely use concentrated HF in our lab. and keep calcium gluconate gel
for first treatment. However, be aware that it is a first treatment after a
water rinse and you should get to a hospital ASAP or risk losing some tissue
or worse.

Here are the details:

Manufactured by:

Calium Gluconate Gel

Calgonate Corp.
Rhode Island, USA

Distributed by:

Pharmascience Inc.
Montreal, Canada

You may call 1-800-363-8805 for info.

Regards,

Peter Tomic
Anadigics, Inc.
Warren, New Jersey
The United States of America



-----Original Message-----
} From: Kai Lorcharoensery [mailto:kai-at-lehigh.edu]
Sent: Thursday, September 19, 2002 2:36 PM
To: microscopy-at-sparc5.microscopy.com


The gel is calcium gluconate. You can buy a few tubes from Fisher (HF
antidote gel) or Pharmascience.

Kai Lorcharoensery
Materials Science & Engineering
Lehigh University
5 E. Packer Ave.
Bethlehem, PA 18015

"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com wrote:

} Hi Folks I am curious if any of you using HF are utilizing the hf antidote
gel. If you are where can I find some in US. Thank You
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}



From daemon Fri Sep 20 08:34:46 2002



From: Greg Mulhollan :      mulhollan-at-extremedevices.com
Date: Fri, 20 Sep 2002 08:27:38 -0500
Subject: RE: SF6 safety

Contents Retrieved from Microscopy Listserver Archives
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Mick Thomas was inquiring about SF6 safety. Ah, that brings back such
fond memories. Back in my days at SLAC, we were using SF6 as the high
voltage insulating gas for our polarized electron gun. We spent a good
deal of time determining risk levels since the gun operated in a
relatively unventilated underground tunnel separated from the other
sections of the accelerator by large doors. Here are a few of the items
we determined:
1. SF6 is an asphyxiant and not a poison. In its pure form it is pretty
unreactive.
2. SF6 is quite heavy and will immediately sink to floor level if there
are in intervening flow barriers. You can consider SF6 as being trapped
somewhere if you think of the analogous amount of water being poured
from or contained in a vessel. In other words, if you have a big vessel
filled with SF6 and open the top, it will retain a good deal of the SF6
for quite some time and still contain very little oxygen!
3. Filling our gun injector room with one complete large bottle of SF6
resulted in only about one inch of an oxygen depleted layer at floor
level.
4. SF6 after having been in the presence of high voltage arcing, will
break down forming some pretty nasty fluorimers, but generally at low
concentrations.

In summary: SF6, due to its weight and (usual) inert qualities presents
little risk, except if it is trapped in something or is broken down to
its sub-components. Hope that helps.

Greg M.

} Fellow microscopists,
}
} I would be very grateful if someone could offer me advice on how to
} ensure that a microscopy room is set up to safely handle a leak of } SF6.
}
} Thank you very much for your time and help.
}
} Sincerely,
}
} Mick
}
} -------------------------------------------------
} Mick Thomas
} UHV-STEM Laboratory
} E-1 Clark Hall
} Cornell University
} Ithaca, NY 14853
}

Gregory Mulhollan, Ph.D.
Senior Scientist
Extreme Devices Inc.
3500 ComSouth Drive
Austin, TX 78744
(512)439-3512 voice
(512)439-3487 fax
mulhollan-at-extremedevices.com



From daemon Fri Sep 20 08:38:29 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 20 Sep 2002 09:24:11 -0400
Subject: Re: hf antidote gel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Hi Folks I am curious if any of you using HF are utilizing the hf antidote
} gel. If you are where can I find some in US. Thank You
}
} Robert Fowler
************************
I buy my HF from Aldrich Chemicals.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Sep 20 08:52:17 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 20 Sep 2002 08:45:54 -0500
Subject: SEM film summary

Contents Retrieved from Microscopy Listserver Archives
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Listers,
I arrived at work today to find a number of responses to my earlier post
asking about film for SEM. I have listed them below without reference to
sender.
I also had a number of responses asking why I wanted to use film for
SEM. This is a good question since for 99% of our SEM work we do capture
digital images and they are adequate for most purposes. However, I guess I
am still from the old school. I still find film better than digital if you
want to enlarge images.
In this particular case, we are hoping to purchase a high resolution
FESEM. A goodly percentage (not all) of the samples will be very
small...things like viruses, liposomes, and various nanoparticles with sizes
ranging from 10nm up. Although the newest models of FESEM can resolve these
structures, even though we will also have to deal with cryo conditions as
most samples will be hydrated, there is still the problem of "empty
magnification" when taking the original image. This limit to the useful
magnification when we originally capture the image is the potential problem.
In many cases we envision that we may need to substantially enlarge
single particles to illustrate finer features to our audience. In this
case, using very fine grained film will give us more opportunity to increase
size without concern for pixelization or without having a computer decide
how to insert smaller pixels (as would happen if we just punch in a higher
resolution figure and let the computer interpret). In fact, it surprises me
that for a relatively small sum when considering the cost of the entire
instrument package, many people do not choose to have the option of using
film when purchasing a new high resolution instrument. You may never use it
but you may well wish to have the option sometime down the road.

Again, thanks to all who responded. I may get a few more responses as
the day goes on but wanted to get this off before it gets buried in my in
box. As to final choice of film....I plan to try a number of the suggested
ones and see what fits our needs.

Debby


==============

Tmax is a bit on the contrasty side. For wider range, I prefer
Ilford FP4+ (rated ISO125, shoot at ISO 100).

This film comes in sheets and rolls (120/220). You can
use cut film holders for 4x5 or use a 6x7cm or 6x9cm roll back for
use with the roll film. Horseman or Wista are the best brands
of roll film backs. These slide into the Graflok back where the
Polaroid back was. Remove the ground glass clam shell affair
by pressing the two chrome latches and slide the ground glass
off the photo unit.

Roll film approach is cheap and very easy to use. Not the same
size as 4x5 but you have the option.

==========

I would certainly consider Kodak Tech Pan 4415. Speed is dependent on
development but will be slower than T-Max, around 25-32 and grain structure
is very fine.

==========
I have used Tech pan 120 roll film in the past and found it to be a very
fine
grained film. Of course you don't get positives untill you develop it,
although
you wouldn't get positives with the Tmax 100 sheet film either.

=============
T-max 100 is excellent film which I have used in both 35mm as
well as 4"x5" formats and have been extremely satisfied with the
grain size. I'm not sure if it comes in quick load sheets which
would look just like the polaroid type 55 film and also uses the 545
holder but it probably does. Otherwise just get a few film holders.
They are not going to be around much longer, although people have
been saying this for years.
===========

I have used Kodak TMax 100 in 35mm and 120 (6x7cm) formats for many
years and can thoroughly recommend it.
In 6x7 cm images the film can record all of the data available from
the SEM record monitor. Images are suitable for enlargement to at
least 16x20", often 20x30" and at these mags film grain is much less
important than image noise. I doubt if you would gain very much by
using 5"x4", and the negatives would be much more trouble to process.
However, there is significant loss of image quality when 35mm format
is used. This may be unimportant for images of the size required in
scientific publications, but degrades the quality of exhibition
images.
=============

I used Agfa APX 100 in 120 rollfilm for SEM images. It exist too in 4x5".
So fine as the T-max and much cheapper. For normal use, I develop it with
Rodinal 1+25. For fine grain use, it's better to use Attomal-FF

=============


Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Fri Sep 20 09:22:34 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 20 Sep 2002 10:15:22 -0500
Subject: Dia-Tech

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Robert Fowler wrote:
===========================================
Maybe this is the location you want?

Dia-Tech Corporation
6408 Clinton Highway, Powell, TN 37849
(865) 938-2720
===========================================
This is a non-working number and so far as I know, they are no longer in
busienss.

However, there are several firms that would be able to resharpen diamond
knives made by Dia-Tech including SPI, MicroStar, DDK, Drukker and others.

Chuck

PS: Remember that we are striving to be 100% paperless, therefore there
are no paper copies kept of this correspondence. Please be sure to always
reply by way of "reply" on your software so that the entire string of
correspondence can be kept in one place.
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================














From daemon Fri Sep 20 09:40:00 2002



From: Stephenson, Matthew :      stephenson-at-impactanalytical.com
Date: Fri, 20 Sep 2002 10:33:15 -0400
Subject: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debby,
We routinely examine HIPS material in the TEM. Our method is to stain the
trimmed and faced sample block in liquid Osmium solution (2% aq) before
sectioning. Just place the block in a sealed bottle of solution, only
enough to cover is needed, and leave in your hood overnight. Remove in the
morning into a small bottle of tap water in the hood for 5 minutes or so,
then set the block out in the hood to air dry for a half hour or so. The
block should be much darker.
Room temperature section as you normally would. Make a careful approach,
because the stain will only penetrate for on the order of 10 sections -at-
100nm. 70nm to 90nm sections look very good.
HIPS stains very well with this method. Some HIPS samples will warp,
making approach more difficult, but usually only to an annoying rather than
catastrophic degree. If you have problems, please contact me off-list about
recalcitrant samples.
Sincerely,
Matt

Matthew K. Stephenson
Analytical Associate
Impact Analytical
1910 West Saint Andrews Road
Midland, MI 48640
(989) 832-5555 X506
stephenson-at-impactanalytical.com



-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Thursday, September 19, 2002 1:02 PM
To: message to: MSA list


Listers,
We, being non-material people, need some help with a material sample.
The sample is a high impact polystyrene (HIPS) that contains butadiene
rubber moieties. We need to be able to identify the location of these
rubber moieties and understand that they will stain upon exposure to osmium.
Does anyone have any experience with this type of sample. Would it be
advisable to stain by floating thin sections (on grids) on liquid osmium or
by using osmium vapors? What type of grids would be most desirable to
minimize corrosion by the osmium?

Also, we have a diamond knife made by Diatech in Tennessee. I need to
contact them to obtain information about this knife. Does anyone have a
phone number or other contact information?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Fri Sep 20 09:55:39 2002



From: Claude Grattepain :      claude.grattepain-at-thalesgroup.com
Date: Fri, 20 Sep 2002 17:22:08 +0200
Subject: SEM, EDX, interferences

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I am a beginner in the EDX field. Is there any free software which could
allow to list the possible energie interferences for a given matrix ?
thanks and regards

Claude GRATTEPAIN

Phone : +33 1 6933 91 13 / Fax : +33 1 69 33 07 40
Mobile : +33 6 84 104 123
mailto:claude.grattepain-at-thalesgroup.com
THALES RESEARCH & TECHNOLOGIES FRANCE
L'Orée de Corbeville - 91401 ORSAY - BP 56 - FRANCE
*****************************************************

The information contained in this e-mail/fax and any attachments are the
property of THALES and may be confidential. If you are not the intended
recipient, please notify us immediately, send this message back to us
and destroy it. You are hereby notified that any review, dissemination,
distribution, copying or otherwise use of this e-mail/fax is strictly
prohibited.




From daemon Fri Sep 20 11:34:31 2002



From: William Oxberry :      William_Oxberry-at-downstate.edu
Date: Fri, 20 Sep 2002 12:26:41 -0400
Subject: Re:Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
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Oldrich-

I just cleaned my condenser aperature using metal polish on a cotton swab.
The same I use to clean the wehnelt. It took care of the very heavy
contamination. I sonicated in acetone two changes for 5 min. each and its
now working fine. You cannot do this with thin foil aperatures (ie.
objective aps.)

Good Luck,

Bill




From daemon Fri Sep 20 11:40:12 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Fri, 20 Sep 2002 09:34:21 -0700 (PDT)
Subject: Re: Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oldrich:

Where I work, I have the luxury of a metallographic
lab available. To clean apertures, I simply use
diamond polishing compound and napped cloth. I use
the tip of my finger to move the aperture in the
diamond compound around on the cloth until the
contaminants are gone. It usually takes just a few
seconds. Follow up with ultrasonic cleaning and
rinsing.

I believe this method is far superior and easier than
baking in removing contaminants. Keep in mind there
is always the risk of catching a corner and folding
the aperture, rendering it useless, which hasn't
happened to me yet.

Stu Smalinskas
SKF NATC
Plymouth, Michigan


__________________________________________________
Do you Yahoo!?
New DSL Internet Access from SBC & Yahoo!
http://sbc.yahoo.com


From daemon Fri Sep 20 11:50:17 2002



From: Marvin H Stromer :      mstromer-at-iastate.edu
Date: Fri, 20 Sep 2002 11:43:59 -0500
Subject: Emeritus Membership

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As described in the MSA Bylaws, article 4, section 3, paragraph e, I wish
to apply for emeritus membership in MSA. I have been a member since 1966,
have been President of the Local Affiliated Society in Iowa and will retire
from employment at Iowa State University on December 31, 2002. Please
contact me if you need additional information.

Sincerely,
Marvin H. Stromer




From daemon Fri Sep 20 11:51:54 2002



From: John W. Raffensperger, Jr. :      johnr-at-helwigcp.com
Date: Fri, 20 Sep 2002 11:42:43 -0500
Subject: Software: speech recognition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't know about specifics for the Mac, but have some Wintel specifics
and general information to offer:

The two "leading" packages for the PC are IBM ViaVoice and Dragon
NaturallySpeaking (made by ScanSoft).

Both have a line of products that very in level of sophistication. They
also both offer specialized versions for specific industries, including
Medical and Legal. I'm not sure if they have "scientific" modules or
not. The modules basically add specialized vocabulary.

There is a matrix of capabilities that are common to most voice
recognition software. One side of the matrix is the "training side",
the other is the "function side".

On the function side, there are basically two modes: Command mode, and
dictate mode. Dictate mode allows dictation (e.g. microscope
observations) into an application. Most of the PC based system can
integrate with the common software, as well as just provide "keyboard"
mimicking into any application that accepts text. The integration with
something like Word provides the ability to handle formatting as your
dictating. The command mode provides access to the Windows interface,
and control of applications and the windows environment. Most dictating
capable products include command mode, but you can find "command mode"
only navigators (probably not appropriate in this case).

On the training axis, there is a continuum. In a perfect world, a voice
recognition system could allow anyone to walk up and start using it with
100% accuracy. The problem here is that we don't all have a monotone
voice with perfect Midwest (pick your geographic area) diction. Not to
mention foreign languages and industry specific "jargon" and
terminology. There are programs that do try and do this, and they are
"speaker independent" systems. While these systems are getting better
as processing power and programming techniques get better and faster,
they tend not to be as robust and accurate as systems that provide some
level of training.

The speaker dependent systems will require varying degrees of training
before the system will work. This generally involved reading a
paragraph or two. From this sample paragraph(s), the system builds a
profile of how to recognize the voice. The more training the more
accurate (in general). The better systems will also provide methods for
integrating additional training as you work with the system. They
provide tools for correcting the errors, and track the errors and
corrections, and integrate them into the profile. The level of ease of
use and sophistication of this ongoing training is one of the things
that separate the quality of the packages. With a motion impaired user,
what method and mechanics a given system uses may have a high impact
(positive or negative) on how effective the system is long range.

Also in the "training" axis would be the ability to add vocabulary. In
a specialized application like microscopy, this would be very important.

I'd also add that technology is changing fast. Look at the revision
history of any product you're considering. If it seems static, I would
suggest that you do NOT consider this as "stable" (and hence a positive
thing) in this application. Programming techniques are changed rapidly,
and software that is slow to adapt will be "behind".

I realize that I didn't provide much specific help, but I hope the
background helps.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Tuesday, September 17, 2002 3:26 PM
To: Microscopy-at-sparc5.microscopy.com


I apologize is this is not strictly a microscopy question. A
microscopist colleague/friend of mine is interested in purchasing a
high quality speech recognition software. The individual is
handicapped and does not full use of their hands and would be using
the software for scientific writing (primarily describing microscopy
findings). Although I favor the Macintosh system (since they would
also use the computer for image processing and publishing), I am
looking for the best software to assist this individual. Any
recommendations would be appreciated. Many thanks.

JB

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################



From daemon Fri Sep 20 12:09:39 2002



From: gary.m.brown-at-exxonmobil.com
Date: Fri, 20 Sep 2002 12:02:38 -0500
Subject: Re: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Debby,

Tobias is correct that RuO4 will stain such rubbers. Unfortunately, RuO4 is
not a selective stain the either the rubber or polystyrene components of
HIPS (stains both heavily). OsO4, on the other hand, will stain only the
unsaturated polybutadiene of HIPS. I think that you will find OsO4 much
more useful.

Regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



Tobias Baskin
{BaskinT-at-missou To: microscopy-at-sparc5.microscopy.com, Debby
ri.edu} Sherman {dsherman-at-purdue.edu}
cc:
Subject: Re: OS staining of rubber
09/19/02 03:16
PM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Debbie,
There is some literature suggesting that ruthenium tetroxide
is better than osmium for this kind of thing. There is a paper by
Trent, Scheinbeim, and Couchaman 1983 Macromols 16: 589-598 that is
very informative. My understanding is that it is usual to vapor
stain this kind of sample.
Hope this helps,
Tobias
}
}
}
} Listers,
} We, being non-material people, need some help with a material sample.
} The sample is a high impact polystyrene (HIPS) that contains butadiene
} rubber moieties. We need to be able to identify the location of these
} rubber moieties and understand that they will stain upon exposure to
osmium.
} Does anyone have any experience with this type of sample. Would it be
} advisable to stain by floating thin sections (on grids) on liquid osmium
or
} by using osmium vapors? What type of grids would be most desirable to
} minimize corrosion by the osmium?
}
} Also, we have a diamond knife made by Diatech in Tennessee. I need
to
} contact them to obtain information about this knife. Does anyone have a
} phone number or other contact information?
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological
Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO
USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123







From daemon Fri Sep 20 12:15:41 2002



From: Stephenson, Matthew :      stephenson-at-impactanalytical.com
Date: Fri, 20 Sep 2002 13:09:43 -0400
Subject: Re: Os staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debby,
We routinely examine HIPS material in the TEM. Our method is to stain the
trimmed and faced sample block in liquid Osmium solution (2% aq) before
sectioning. Just place the block in a sealed bottle of solution, only
enough to cover is needed, and leave in your hood overnight. Remove in the
morning into a small bottle of tap water in the hood for 5 minutes or so,
then set the block out in the hood to air dry for a half hour or so. The
block should be much darker.
Room temperature section as you normally would. Make a careful approach,
because the stain will only penetrate for on the order of 10 sections -at-
100nm. 70nm to 90nm sections look very good.
HIPS stains very well with this method. Some HIPS samples will warp,
making approach more difficult, but usually only to an annoying rather than
catastrophic degree. If you have problems, please contact me off-list about
recalcitrant samples.
Sincerely,
Matt

Matthew K. Stephenson
Analytical Associate
Impact Analytical
1910 West Saint Andrews Road
Midland, MI 48640
(989) 832-5555 X506
stephenson-at-impactanalytical.com




From daemon Fri Sep 20 12:58:04 2002



From: Rita Monahan-Earley :      rmonahan-at-caregroup.harvard.edu
Date: Fri, 20 Sep 2002 13:48:34 -0400
Subject: Metal "Boats"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Members of this valuable site,
I am interested in buying some LKB metal boats "Metal Collecting
Troughs" 4815B) to be used on glass knives.

We have a supply in our lab but need more.
If anyone has any leads on where I can order these, it would be much
appreciated.

I'm not adverse to buying pre-owned boats. If they have been used
gently.

Thank you,
Rita


From daemon Fri Sep 20 14:47:35 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 20 Sep 2002 13:02:28 -0700
Subject: Re: SEM film summary

Contents Retrieved from Microscopy Listserver Archives
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Debby

I would completely agree with your point about high resolution film etc if
we are talking about TEM. In TEM, image forming electrons directly
interact with film. So, the quality of the film is a 'limitation factor'
there. In TEM even now the film has some advantages over the digital
camera. The film greatest advantage - as you mentioned in your message, is
ability to enlarge the image without serious pixelation.

In SEM, image forming electrons are detected by some sort of device
("detector"). So, at the output you have actually electrical signal, which
represents the properties of your sample. This electrical signal then
converted back into the image on the CRT. You also may project CRT image
on the film and have a "hard" copy. In such scenario, the image resolution
on the film dependent from the quality of the "projection system" and
actual size of the SEM probe. Because, signal already present in the
"electrical form" (analog or digital) there is no reason to convert it into
the image on the film: with modern image grabbers and high-quality printers
you may directly convert it into hard copy without intermediate (film)
step. In my point of view, this is fundamental difference between SEM and
TEM.

Another point, on the film, you could record about 100 shades of gray
(dynamic range is 10^2), modern digital devices will record 3-6K levels of
grey from the same sample. So, using film in SEM - you lost information,
because original signal was (and is) an electronic and then converted into
the image. When you directly print your image (digitally) you also loose
the dynamic range BUT: you'll still have the original digital image with
all that 6K shades of gray. Using film you automatically reduce the
dynamic range and, also, keep in mind that film is not linear: it records
image in the logarithmic scale... I am sorry... I don't see ANY advantages
of using film in SEM, but I DO see some advantages of using film in
TEM. Have a good day. Sergey



At 08:45 AM 9/20/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Fri Sep 20 15:11:07 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 20 Sep 2002 14:04:18 -0400
Subject: seeking a job

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
I was contacted by a Post-Doctoral fellow who is seeking a part-time
technical position in an EM lab in the NYC area. Her CV is available
upon direct request to her. Please respond to her directly, off-line.
Lee


ljiljan minwalla, PhD
email: minwal01-at-med.nyu.edu
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Sep 20 15:29:02 2002



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Fri, 20 Sep 2002 15:27:35 -0500
Subject: Re: SF6 safety

Contents Retrieved from Microscopy Listserver Archives
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Mick

Most States do not have specific requirements for SF6 handling as it is
classed as an inert gas. However it is an asphyxiant and will settle to
the floor replacing oxygen. Therefore any labs with a significant amount
of SF6 (T tank or more) should be well ventilated with a floor level vent
to the outside of the building. In general EM labs tend to be small and
not that well ventilated at ground level. In our case we have 3.5" pipe at
ground level, near the tank storage and HT generator, tied into a fume hood
plenum which has a fan capable of sufficient draught to remove any SF6 spilled.

It does have hazardous decomposition products either generated by electric
arcs (SF4, SOF2, SO2F2, OF2 and HF) or heating in the presence of steels
which act as a catalyst above 200degC (SF2, SF4, S2F10), however in the
quantities used, contained, in microscopy the potential for generation of
hazardous byproducts is small.

Regards

Alan

At 01:08 PM 9/19/2002 -0400, Mick Thomas wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Fri Sep 20 15:29:02 2002



From: Russell E. Cook :      recook-at-anl.gov
Date: Fri, 20 Sep 2002 15:23:09 -0500
Subject: RE: SF6 safety

Contents Retrieved from Microscopy Listserver Archives
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We had a large pit under the now-decommissioned HVEM here at Argonne. The
pit had a sensor to detect oxygen levels. If the oxygen level dropped
below a set point an exhaust fan was started and a warning horn sounded.
You shouldn't have to do that. As Greg has pointed out, you'd have to be
prone on the floor - face down - before you'd be asphyxiated by the typical
setup these days. Nevertheless, you might want to have a small exhaust fan
to the building exterior installed at floor level so that you can eliminate
as much SF6 as possible in the event of a serious leak. Talk to your
institutional safety people.

We also have 1 inch copper pipes installed in the microscope rooms to
exhaust/dump SF6 to the building exterior before servicing acceleration
chambers or HV tanks. The outlet of the pipes are about 3 to 4 feet above
the ground and have a perforated cap to keep out insects. We've also
posted warning signs near the outlets.

Russell E. Cook, Ph.D.
Electron Microscopy Center
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov
} -----------------------------------------------------------------------
} Date: Fri, 20 Sep 2002 08:27:38 -0500
} Subject: RE: SF6 safety
} From: Greg Mulhollan {mulhollan-at-extremedevices.com}
}
} Mick Thomas was inquiring about SF6 safety. Ah, that brings back such
} fond memories. Back in my days at SLAC, we were using SF6 as the high
} voltage insulating gas for our polarized electron gun. We spent a good
} deal of time determining risk levels since the gun operated in a
} relatively unventilated underground tunnel separated from the other
} sections of the accelerator by large doors. Here are a few of the items
} we determined:
} 1. SF6 is an asphyxiant and not a poison. In its pure form it is pretty
} unreactive.
} 2. SF6 is quite heavy and will immediately sink to floor level if there
} are in intervening flow barriers. You can consider SF6 as being trapped
} somewhere if you think of the analogous amount of water being poured
} from or contained in a vessel. In other words, if you have a big vessel
} filled with SF6 and open the top, it will retain a good deal of the SF6
} for quite some time and still contain very little oxygen!
} 3. Filling our gun injector room with one complete large bottle of SF6
} resulted in only about one inch of an oxygen depleted layer at floor
} level.
} 4. SF6 after having been in the presence of high voltage arcing, will
} break down forming some pretty nasty fluorimers, but generally at low
} concentrations.
}
} In summary: SF6, due to its weight and (usual) inert qualities presents
} little risk, except if it is trapped in something or is broken down to
} its sub-components. Hope that helps.
}
} Greg M.
}
} } Fellow microscopists,
} }
} } I would be very grateful if someone could offer me advice on how to
} } ensure that a microscopy room is set up to safely handle a leak of } SF6.
} }
} } Thank you very much for your time and help.
} }
} } Sincerely,
} }
} } Mick
} }
} } -------------------------------------------------
} } Mick Thomas
} } UHV-STEM Laboratory
} } E-1 Clark Hall
} } Cornell University
} } Ithaca, NY 14853
} }
}
} Gregory Mulhollan, Ph.D.
} Senior Scientist
} Extreme Devices Inc.
} 3500 ComSouth Drive
} Austin, TX 78744
} (512)439-3512 voice
} (512)439-3487 fax
} mulhollan-at-extremedevices.com
}




From daemon Fri Sep 20 15:44:54 2002



From: Steve Chapman :      protrain-at-emcourses.com
Date: Fri, 20 Sep 2002 21:22:39 +0100
Subject: Re: Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

The standard way to clean molybdenum apertures is in a high vacuum coating
unit, heating the apertures to orange-red and holding them at that
temperature until the deposit(s) evaporate.

Heating in a sputter coater or in a flame does not work with molybdenum.
Heating to white heat is not a good idea as this often distorts the aperture
or, if you try to re use the cleaned apertures and clean them again grain
growth also tends to spoil the aperture shape.

If you are unable to clean the apertures by the first method described you
are better off discarding them; dirty apertures are not a good idea!

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com




From daemon Fri Sep 20 15:56:10 2002



From: simkin-at-egr.msu.edu
Date: Fri, 20 Sep 2002 16:47:00 -0400 (EDT)
Subject: Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have had someone come to me asking about microscopic methods of reading
data on hard disk platters. Does anyone have experience along these lines,
or suggestions of who may have done this sort of thing? The question seems to
deal mainly with the persistence of the magnetic domains, and what sort of
'memory' the disk maintains during multiple writing operations.

Any suggestions would me greatly appreciated.

Ben Simkin (simkin-at-egr.msu.edu)
Michigan State University




From daemon Fri Sep 20 16:08:56 2002



From: saram-at-duke.edu
Date: Fri, 20 Sep 2002 17:00:09 -0400 (EDT)
Subject: Nikon shutter device for grabs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have uncovered a box ~4x5x2 in that looks like an exposure device for a
Nikon photomicroscope. It has selections for manual/auto, speed, ASA,
time, and over/under adjustments. It has a cord that fastens to the
camera with 8 pins around the periphery and 2 in the center. If anyone
can use it, I will mail it. Otherwise it goes into the dump.

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Sep 20 16:18:14 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Fri, 20 Sep 2002 17:13:15 -0400
Subject: JEOL 2010F

Contents Retrieved from Microscopy Listserver Archives
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How many JEOL 2010Fs are there out there? If you have one I would
appreciate it if you would drop me a line directly and not to the list.
Thanks.

--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"




From daemon Fri Sep 20 16:41:16 2002



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Fri, 20 Sep 2002 17:34:27 -0400
Subject: Re: Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oldrich,

If the apertures are from a Philips TEM, they are most likely made of
Platinum. You don't heat these in vacuum as you do the Mo
apertures. These can be cleaned by heating them in a Pt basket over a
bunsen burner in air. The oxygen in air won't oxidize the Pt, but will
remove the hydrocarbon contamination quite effectively.

If you look in your CM12 owners book, Philips should have a section on
maintenance where they describe the procedure.

Cheers,
Henk Colijn


At 08:30 AM 9/20/2002 +0200, =?ISO-8859-2?Q?Old=F8ich_Benada?= wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Fri Sep 20 17:13:58 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 20 Sep 2002 17:07:24 -0500
Subject: Re: SEM film & M&M2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ann and others,
I am glad that you have found the MSA Facility Management series useful.
Unfortunately it may be held less often in the future. Due to the large
number of annually repeating sessions that limit addition of new sessions,
it looks like it will be relegated to every other year starting immediately
although there is no guarantee that it will be continued in the future. At
least there will be no support yearly so speakers will have to be gleaned
from those already going to the meetings. This is usually the case for most
anyway but has not been the case for all.
We will try to limp along if we can find appropriate facilitators
already going to the meeting and a room to hold the session. Hopefully we
will still get funding for the audio-visual required.
In any case let's be optimistic and plan on a session at M&M2003. With
that, I have a few ideas as a topic but, since this is meant to be relative
to all you lab managers out there, what would you like to have
presented/discussed at the next session? Please let me know with
suggestions for facilitators as well.

Debby



On 9/20/02 11:32 AM, "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} wrote:

} Hi Debbie,
}
} Many moons ago (in the 80's) I regularly used Kodak Ektapan sheet film in
} 4x5 carriers inserted into the Polaroid holder, and got good results. Tech
} Pan sounds promising though.
}
} I can appreciate your desire to get good negatives, as I am struggling with
} an intro SEM course in which my students are only exposed to digital imaging
} (no pun intended). I feel I am short-changing them.
}
} Let us know what you learn!!
}
} And thanks again for organizing the MSA facility management series.
}
} cheers,
} Ann
}
} ###################
} Ann Hein Lehman
} Assistant Director, EM Facility
} Trinity College - LSC314
} 300 Summit Street
} Hartford CT 06106
} v. 860-297-4289
} f. 860-297-2538
} e. ann.lehman-at-trincoll.edu
} w. http://www.trincoll.edu/~alehman/
} Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
}



From daemon Fri Sep 20 17:13:59 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 20 Sep 2002 15:07:02 -0700
Subject: Re: SEM film summary

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Debby,
The limitation of resolution from an SEM image is not the fine grain of the
film, but the line resolution of the SEM and photo CRT and the ability of
the photo CRT and camera to resolve those lines. While you can enlarge a
TEM image ten times because the image is a real image and the film can still
resolve features at that enlargement, the SEM image will start to show you
the lines in the SEM CRT above about five times enlargement. If you do not
see the lines, then your SEM CRT is not focussing even that well and your
image will be degraded further. Fine film won't help that. The highest
resolution SEM photo CRT I have seen is 2500 lines and so that is the most
lines that the SEM will put out. There is no reason that a 2500 line image
from the film camera is going to be any better or sharper or higher
resolution than a 2500 line digital image. The only choice is the output
media and, with glossy photo paper, an high-resolution inkjet printer will
give you a two minute 8 X 10 print. There is nothing magic about film or
inferior about digital imaging.
At 08:45 AM 09/20/2002 -0500, you wrote:

} Listers,
} I arrived at work today to find a number of responses to my earlier post
} asking about film for SEM. I have listed them below without reference to
} sender.
} I also had a number of responses asking why I wanted to use film for
} SEM. This is a good question since for 99% of our SEM work we do capture
} digital images and they are adequate for most purposes. However, I guess I
} am still from the old school. I still find film better than digital if you
} want to enlarge images.
} In this particular case, we are hoping to purchase a high resolution
} FESEM. A goodly percentage (not all) of the samples will be very
} small...things like viruses, liposomes, and various nanoparticles with sizes
} ranging from 10nm up. Although the newest models of FESEM can resolve these
} structures, even though we will also have to deal with cryo conditions as
} most samples will be hydrated, there is still the problem of "empty
} magnification" when taking the original image. This limit to the useful
} magnification when we originally capture the image is the potential problem.
} In many cases we envision that we may need to substantially enlarge
} single particles to illustrate finer features to our audience. In this
} case, using very fine grained film will give us more opportunity to increase
} size without concern for pixelization or without having a computer decide
} how to insert smaller pixels (as would happen if we just punch in a higher
} resolution figure and let the computer interpret). In fact, it surprises me
} that for a relatively small sum when considering the cost of the entire
} instrument package, many people do not choose to have the option of using
} film when purchasing a new high resolution instrument. You may never use it
} but you may well wish to have the option sometime down the road.
}
} Again, thanks to all who responded. I may get a few more responses as
} the day goes on but wanted to get this off before it gets buried in my in
} box. As to final choice of film....I plan to try a number of the suggested
} ones and see what fits our needs.
}
} Debby
}
..snip

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Sep 20 17:44:23 2002



From: saram-at-duke.edu
Date: Fri, 20 Sep 2002 19:44:03 -0400 (EDT)
Subject: Shutter control

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Several years ago (4-5) we looked at this issue in regards to handling
rodent filth & concerns about hantavirus, etc. From the literature search
we found the following (which may have changed) when looking at latex vs.
nitrile gloves:

1. No known allergic reactions to nitrile.
2. Nitrile gloves had smaller pores than latex gloves.
3. Nitrile gloves had a lower failure rate during testing than latex gloves.

We switched to nitrile gloves for "filth" work.

These are my own
comments & not of my employer.
David A. Foran, chemist
Food and Drug Administration
Kansas City Elemental Analysis Lab
DFORAN-at-ORA.FDA.GOV
913-752-2170
913-752-2727 (voice mail)
913-752-2151 (fax)


-----Original Message-----
} From: "Frida.Maiers-at-co.hennepin.mn.us"-at-sparc5.microscopy.com
[mailto:"Frida.Maiers-at-co.hennepin.mn.us"-at-sparc5.microscopy.com]
Sent: Wednesday, September 18, 2002 1:30 PM
To: microscopy-at-sparc5.microscopy.com


The shutter control has found a home.

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Sep 20 20:09:26 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 20 Sep 2002 21:10:40 -0400
Subject: Re: SEM film summary

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mary,

It gets worse than that : c.a. 1985 when working on a SEM in a colleagues lab I
noticed that the horizontal scan lines that were normally distinguishable at
certain faster scan settings on my instrument were not visible on his (same mfg.,
same scan module, same recording CRT, different chamber and spectrometer setup).
When I inquired, he told me that the mfg.'s service rep had been there only the
day before and completely optimized everything, including readjusting the
brightness and contrast settings on the recording CRT.

After a little investigation I found that the tech had decreased the brightness
settings and compensated by opening the photo transfer lens iris from f-8 to
f-5.6. I had already learned from experience that an iris opening this wide led
to a a defocused image of the CRT spot on the film which could not be corrected
by any adjustment of the mechanical focus.

When asked why he had intentionally defocused the image, the tech responded that
the faintly visible scan lines on fast frame rates annoyed some customers so he
made them happy by effectively blurring the image so that the raster lines ran
together!!! (Names withheld to protect the guilty)

Part of the point of this story is that the vertical resolution on a SEM may be
limited by the number of lines in the raster pattern rather than the film or the
camera system. On a high resolution CRT with type 55 P/N film the individual
lines were often discernable. However, the horizontal resolution is limited only
by the instrument response factors, imaging conditions and the film. To ensure
that optimal resolution is maintained, one may have to live with some raster
artifacts in the vertical direction if, for example, a fast scan rate is required
due to an unstable sample.

John Twilley
Conservation Scientist

Mary Mager wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Debby,
} The limitation of resolution from an SEM image is not the fine grain of the
} film, but the line resolution of the SEM and photo CRT and the ability of
} the photo CRT and camera to resolve those lines. While you can enlarge a
} TEM image ten times because the image is a real image and the film can still
} resolve features at that enlargement, the SEM image will start to show you
} the lines in the SEM CRT above about five times enlargement. If you do not
} see the lines, then your SEM CRT is not focussing even that well and your
} image will be degraded further. Fine film won't help that. The highest
} resolution SEM photo CRT I have seen is 2500 lines and so that is the most
} lines that the SEM will put out. There is no reason that a 2500 line image
} from the film camera is going to be any better or sharper or higher
} resolution than a 2500 line digital image. The only choice is the output
} media and, with glossy photo paper, an high-resolution inkjet printer will
} give you a two minute 8 X 10 print. There is nothing magic about film or
} inferior about digital imaging.



From daemon Fri Sep 20 21:52:28 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 20 Sep 2002 19:48:15 -0700
Subject: Re: SEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The ability of film to record SEM or TEM data has to be compared
to the scan rate density of SEM (2K) and TEM (integrated) versus
that of the actual film's capabilities.

A finer grain film in a SEM environment at 2K lpi is not going to
be all that impressive compared to a direct digital capture at
4Kx4K. the other factor in favor of digital capture is realtime
evaluation of the histogram of the total image. One can optimize
contrast and brightness to collect a perfect image. Using film, this
is hit or miss at best.

gary g.


At 03:07 PM 9/20/2002, you wrote:

} Ann and others,
} I am glad that you have found the MSA Facility Management series useful.
} Unfortunately it may be held less often in the future. Due to the large
} number of annually repeating sessions that limit addition of new sessions,
} it looks like it will be relegated to every other year starting immediately
} although there is no guarantee that it will be continued in the future. At
} least there will be no support yearly so speakers will have to be gleaned
} from those already going to the meetings. This is usually the case for most
} anyway but has not been the case for all.
} We will try to limp along if we can find appropriate facilitators
} already going to the meeting and a room to hold the session. Hopefully we
} will still get funding for the audio-visual required.
} In any case let's be optimistic and plan on a session at M&M2003. With
} that, I have a few ideas as a topic but, since this is meant to be relative
} to all you lab managers out there, what would you like to have
} presented/discussed at the next session? Please let me know with
} suggestions for facilitators as well.
}
} Debby
}
}
}
} On 9/20/02 11:32 AM, "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} wrote:
}
} } Hi Debbie,
} }
} } Many moons ago (in the 80's) I regularly used Kodak Ektapan sheet film in
} } 4x5 carriers inserted into the Polaroid holder, and got good results. Tech
} } Pan sounds promising though.
} }
} } I can appreciate your desire to get good negatives, as I am struggling with
} } an intro SEM course in which my students are only exposed to digital
} imaging
} } (no pun intended). I feel I am short-changing them.
} }
} } Let us know what you learn!!
} }
} } And thanks again for organizing the MSA facility management series.
} }
} } cheers,
} } Ann
} }
} } ###################
} } Ann Hein Lehman
} } Assistant Director, EM Facility
} } Trinity College - LSC314
} } 300 Summit Street
} } Hartford CT 06106
} } v. 860-297-4289
} } f. 860-297-2538
} } e. ann.lehman-at-trincoll.edu
} } w. http://www.trincoll.edu/~alehman/
} } Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
} }



From daemon Fri Sep 20 22:35:07 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Fri, 20 Sep 2002 22:27:22 -0500
Subject: Re: SF6 safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg,

This also brings back some not so fond memories of SF6 for a HVEM microscope
where is was used in the high voltage tanks. When we had to open the tanks
for service, the gas had to be pumped into underground storage tanks. When
we were finished with the repairs, the SF6 was pumped back into the
accelerator and generator through molecular sieve to remove moisture. This
meant that I had to then vacuum the filters to remove the powdered molecular
sieve material formed as the gas blasted through them. From the noise it
may be been at supersonic speeds, at least to begin with. I think that the
powdered molecular sieve dust can be dangerous to breathe into your lungs
and it gets everywhere and when vacuuming I had to ground myself, the vacuum
and the filter holder.

Damian Neuberger.

} Mick Thomas was inquiring about SF6 safety. Ah, that brings back such
} fond memories. Back in my days at SLAC, we were using SF6 as the high
} voltage insulating gas for our polarized electron gun. We spent a good
} deal of time determining risk levels since the gun operated in a
} relatively unventilated underground tunnel separated from the other
} sections of the accelerator by large doors. Here are a few of the items
} we determined:
} 1. SF6 is an asphyxiant and not a poison. In its pure form it is pretty
} unreactive.
} 2. SF6 is quite heavy and will immediately sink to floor level if there
} are in intervening flow barriers. You can consider SF6 as being trapped
} somewhere if you think of the analogous amount of water being poured
} from or contained in a vessel. In other words, if you have a big vessel
} filled with SF6 and open the top, it will retain a good deal of the SF6
} for quite some time and still contain very little oxygen!
} 3. Filling our gun injector room with one complete large bottle of SF6
} resulted in only about one inch of an oxygen depleted layer at floor
} level.
} 4. SF6 after having been in the presence of high voltage arcing, will
} break down forming some pretty nasty fluorimers, but generally at low
} concentrations.
}
} In summary: SF6, due to its weight and (usual) inert qualities presents
} little risk, except if it is trapped in something or is broken down to
} its sub-components. Hope that helps.




From daemon Fri Sep 20 23:08:34 2002



From: Allen Sampson :      ars-at-sem.com
Date: Fri, 20 Sep 2002 23:04:33 -0700
Subject: RE: Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Twenty years ago I helped design an SEM for IBM's storage products division
in Rochester, MN (I don't think they exist anymore, at least not there).
This was back in the days of the 12" platters and this instrument was
designed to enable imaging of any portion of the disk at high tilt angles
(biggest sample chamber ever made, as far as I know).

Having said that, I have to question the source of the question.
Recovering data from hard drives that has been written over is really only
done for one legitimate reason - law enforcement, and one perhaps
self-legitimizing reason - intelligence gathering. Data recovery services
would not normally attempt anything so esoteric, and customers don't come
to them for recovering such data. They generally handle bad drive
controller electronics and head crashes that have destroyed sector data -
wanting to recover recent data. There is no data that an individual would
have that would be worth the effort, even if they lost business informat
ion. Far easier and less expensive to reconstruct it by other means.

With that head's up, I'll just say that the positioning of the heads is not
perfect. They do not write over the exact same area every time. The
persistence of the domains is very long under normal operating conditions.
Finding the anomalies and producing a coherent representation of the
underlying data is the problem - it's not easy and the ability to reproduce
sizable chunks of data depends on many variables.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Friday, September 20, 2002 1:47 PM,
"simkin-at-egr.msu.edu"-at-sparc5.microscopy.com
[SMTP:"simkin-at-egr.msu.edu"-at-sparc5.microscopy.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
}
} I have had someone come to me asking about microscopic methods of
reading
} data on hard disk platters. Does anyone have experience along these
lines,
} or suggestions of who may have done this sort of thing? The question
seems to
} deal mainly with the persistence of the magnetic domains, and what sort
of
} 'memory' the disk maintains during multiple writing operations.
}
} Any suggestions would me greatly appreciated.
}
} Ben Simkin (simkin-at-egr.msu.edu)
} Michigan State University
}
}
}
}



From daemon Sat Sep 21 01:19:52 2002



From: Allen Sampson :      ars-at-sem.com
Date: Sat, 21 Sep 2002 01:12:09 -0700
Subject: RE: SEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm not going to argue the merits of digital vs. analog at this point.
Gary's point about the histogram has caught my interest.

Many SEM's offer the ability to view an analog 'waveform' (each
manufacturer seems to call it by a different name). This is essentially an
oscillographic display of the signal intensity. It is a dynamic display of
each scan line's signal as opposed to the static histogram of most image
capture systems which offer a representation of the sum of all scan lines.
The difference is that an accomplished operator may adjust the brightness
and contrast to a particular feature of interest, rather than the total
frame. You could put a digital system in line mode and manually run the
line position up and down to characterize the feature of interest, but with
the waveform display you can see this with every frame, as well as the
overall signal frame response.

Just a thought.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Friday, September 20, 2002 7:48 PM, Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The ability of film to record SEM or TEM data has to be compared
} to the scan rate density of SEM (2K) and TEM (integrated) versus
} that of the actual film's capabilities.
}
} A finer grain film in a SEM environment at 2K lpi is not going to
} be all that impressive compared to a direct digital capture at
} 4Kx4K. the other factor in favor of digital capture is realtime
} evaluation of the histogram of the total image. One can optimize
} contrast and brightness to collect a perfect image. Using film, this
} is hit or miss at best.
}
} gary g.
}
}
} At 03:07 PM 9/20/2002, you wrote:
}
} } Ann and others,
} } I am glad that you have found the MSA Facility Management series
useful.
} } Unfortunately it may be held less often in the future. Due to the large
} } number of annually repeating sessions that limit addition of new
sessions,
} } it looks like it will be relegated to every other year starting
immediately
} } although there is no guarantee that it will be continued in the future.
At
} } least there will be no support yearly so speakers will have to be
gleaned
} } from those already going to the meetings. This is usually the case for
most
} } anyway but has not been the case for all.
} } We will try to limp along if we can find appropriate facilitators
} } already going to the meeting and a room to hold the session. Hopefully
we
} } will still get funding for the audio-visual required.
} } In any case let's be optimistic and plan on a session at M&M2003.
With
} } that, I have a few ideas as a topic but, since this is meant to be
relative
} } to all you lab managers out there, what would you like to have
} } presented/discussed at the next session? Please let me know with
} } suggestions for facilitators as well.
} }
} } Debby
} }
} }
} }
} } On 9/20/02 11:32 AM, "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} wrote:
} }
} } } Hi Debbie,
} } }
} } } Many moons ago (in the 80's) I regularly used Kodak Ektapan sheet
film in
} } } 4x5 carriers inserted into the Polaroid holder, and got good results.
Tech
} } } Pan sounds promising though.
} } }
} } } I can appreciate your desire to get good negatives, as I am
struggling with
} } } an intro SEM course in which my students are only exposed to digital
} } imaging
} } } (no pun intended). I feel I am short-changing them.
} } }
} } } Let us know what you learn!!
} } }
} } } And thanks again for organizing the MSA facility management series.
} } }
} } } cheers,
} } } Ann
} } }
} } } ###################
} } } Ann Hein Lehman
} } } Assistant Director, EM Facility
} } } Trinity College - LSC314
} } } 300 Summit Street
} } } Hartford CT 06106
} } } v. 860-297-4289
} } } f. 860-297-2538
} } } e. ann.lehman-at-trincoll.edu
} } } w. http://www.trincoll.edu/~alehman/
} } } Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
} } }
}
}
}
}



From daemon Sat Sep 21 21:19:31 2002



From: simkin-at-egr.msu.edu
Date: Sat, 21 Sep 2002 22:05:12 -0400 (EDT)
Subject: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just some further information to settle fears-

The person whom I received the question from is an old childhood friend with
a strong curiosity. He is one of the computer geeks for a local programming
concern.
The thing that piqued his interest was an assertion that it required a
minimum of 7 writing cycles to obscure old drive data, so he wants to test it.
I confess myself surprised by this, so now I'm interested as well. I would
not have expected much of anything to have survived more than 3 writes.
I find myself wondering if (beyond track wander) there is less than perfect
flipping of domains in the entire area of a bit. (Of course I could be way out
on a limb here because this is isn't my area of expertise by a long shot.)

Ben Simkin (simkin-at-egr.msu.edu)
Michigan State University



} Twenty years ago I helped design an SEM for IBM's storage products division
} in Rochester, MN (I don't think they exist anymore, at least not there).
} This was back in the days of the 12" platters and this instrument was
} designed to enable imaging of any portion of the disk at high tilt angles
} (biggest sample chamber ever made, as far as I know).
}
} Having said that, I have to question the source of the question.
} Recovering data from hard drives that has been written over is really only
} done for one legitimate reason - law enforcement, and one perhaps
} self-legitimizing reason - intelligence gathering. Data recovery services
} would not normally attempt anything so esoteric, and customers don't come
} to them for recovering such data. They generally handle bad drive
} controller electronics and head crashes that have destroyed sector data -
} wanting to recover recent data. There is no data that an individual would
} have that would be worth the effort, even if they lost business informat
} ion. Far easier and less expensive to reconstruct it by other means.
}
} With that head's up, I'll just say that the positioning of the heads is not
} perfect. They do not write over the exact same area every time. The
} persistence of the domains is very long under normal operating conditions.
} Finding the anomalies and producing a coherent representation of the
} underlying data is the problem - it's not easy and the ability to reproduce
} sizable chunks of data depends on many variables.
}
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} } Hi all,
} }
} } I have had someone come to me asking about microscopic methods of
} reading
} } data on hard disk platters. Does anyone have experience along these
} lines,
} } or suggestions of who may have done this sort of thing? The question
} seems to
} } deal mainly with the persistence of the magnetic domains, and what sort
} of
} } 'memory' the disk maintains during multiple writing operations.
} }
} } Any suggestions would me greatly appreciated.
} }
} } Ben Simkin (simkin-at-egr.msu.edu)
} } Michigan State University


From daemon Sun Sep 22 02:09:19 2002



From: Allen Sampson :      ars-at-sem.com
Date: Sun, 22 Sep 2002 03:43:44 -0700
Subject: RE: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Ben:

We work on disk platter and head everyday. It is impossible to "see"
magnetic domain on optical microscope, neither SEMs. On a disk with data
density of 10-20GB/side of platter, the width of track is ~0.4 um and
simular scale on the length, the thickness of magnetic layer is 10-30 nm.
It is too small for optical microscope and too thin for SEM to get enough
mass (for holding magnetic energy) for SEI signal.

AFM with magnetic tip is one good tool to see the magnetic field image
(field distribution map). Another instrument, call Optical Surface Analyzer
(OSA) is another good choice to see the magnetic domain. The resolution can
not reach single domain, just see the data stream as noise waveform.

When you open a hard drive without clean toom envionment, the surface of
disk would be contaminated by floating particles (dust). You will no longer
be able to see the original surface.

Zhiyu Wang
Maxtor Corp.
zhiyu_wang-at-maxtor.com







----- Original Message -----
} From: {"simkin-at-egr.msu.edu"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, September 20, 2002 8:47 PM


Suppose, just suppose, that a magnetic head does not do a complete job of
magnetizing a particular group of grains in the thin film of a hard drive
disk - it does, however, change it enough to be recognizable when that
portion is read. If the film had at first been completely demagnetized,
the first write would leave it at some percentage of complete polarization.
A second write at that site would then leave one of two states -
augmentation of the original write if the polarity was the same or a
reversal of polarity. In the first case, the group has a slightly higher
magnetization in the same direction, in the latter a slightly lessor
magnetization in the opposite direction. Double those two states since
there are two polarities to the magnetic charge, and you have four separate
possible states.

When a third write operation is made, eight different states could be
imagined. In the case where the write is of the same polarity as the first
and second, the magnetization is again enhanced. When the current write
opposes the previous two, the group is left at a lower state of opposite
polarity. If the current write opposes the previous write which opposed
the first write, the magnetization is lessened and opposite in polarity.
And finally, if the current write is of the same polarity as the previous
write and opposite of the first write, the previous state is enhanced.
Once again, double those states as we are dealing with two polarization
states.

While it may seem from the above that there is a continuing reduction in
the magnetic charge, statistically there is a 50% chance that any
particular write will increase the magnetic charge, so in the long run
there is a predictable magnetic charge, in either polarity, on this group
of magnetic grains, and thus a reliable level at which one can determine
the difference between states of the last write.

The question really becomes one of the resolution available to read these
individual charges and the amount of computational power available to
deconvolute the possible states. Three writes is generally enough to
satisfy the normal paranoid personality. For those concerned with the
black helicopters outside their windows and nightmares of NSA, seven writes
may suffice. But the technology of US government agencies should never be
underestimated.

Why this situation might occur is a question of economics and physics. We
want the most data storage available per linear unit of hard drive space.
There is an overlap between what works reliably and what leaves detectable
traits.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Saturday, September 21, 2002 7:05 PM,
"simkin-at-egr.msu.edu"-at-sparc5.microscopy.com
[SMTP:"simkin-at-egr.msu.edu"-at-sparc5.microscopy.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Just some further information to settle fears-
}
} The person whom I received the question from is an old childhood
friend with
} a strong curiosity. He is one of the computer geeks for a local
programming
} concern.
} The thing that piqued his interest was an assertion that it required a
} minimum of 7 writing cycles to obscure old drive data, so he wants to
test it.
} I confess myself surprised by this, so now I'm interested as well. I
would
} not have expected much of anything to have survived more than 3 writes.
} I find myself wondering if (beyond track wander) there is less than
perfect
} flipping of domains in the entire area of a bit. (Of course I could be
way out
} on a limb here because this is isn't my area of expertise by a long
shot.)
}
} Ben Simkin (simkin-at-egr.msu.edu)
} Michigan State University
}
}
}
} } Twenty years ago I helped design an SEM for IBM's storage products
division
} } in Rochester, MN (I don't think they exist anymore, at least not there).
} } This was back in the days of the 12" platters and this instrument was
} } designed to enable imaging of any portion of the disk at high tilt
angles
} } (biggest sample chamber ever made, as far as I know).
} }
} } Having said that, I have to question the source of the question.
} } Recovering data from hard drives that has been written over is really
only
} } done for one legitimate reason - law enforcement, and one perhaps
} } self-legitimizing reason - intelligence gathering. Data recovery
services
} } would not normally attempt anything so esoteric, and customers don't
come
} } to them for recovering such data. They generally handle bad drive
} } controller electronics and head crashes that have destroyed sector data
-
} } wanting to recover recent data. There is no data that an individual
would
} } have that would be worth the effort, even if they lost business informat
} } ion. Far easier and less expensive to reconstruct it by other means.
} }
} } With that head's up, I'll just say that the positioning of the heads is
not
} } perfect. They do not write over the exact same area every time. The
} } persistence of the domains is very long under normal operating
conditions.
} } Finding the anomalies and producing a coherent representation of the
} } underlying data is the problem - it's not easy and the ability to
reproduce
} } sizable chunks of data depends on many variables.
} }
} }
} } Allen R. Sampson
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} }
} } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
} }
} }
} } } Hi all,
} } }
} } } I have had someone come to me asking about microscopic methods of
} } reading
} } } data on hard disk platters. Does anyone have experience along these
} } lines,
} } } or suggestions of who may have done this sort of thing? The question
} } seems to
} } } deal mainly with the persistence of the magnetic domains, and what
sort
} } of
} } } 'memory' the disk maintains during multiple writing operations.
} } }
} } } Any suggestions would me greatly appreciated.
} } }
} } } Ben Simkin (simkin-at-egr.msu.edu)
} } } Michigan State University
}
}
}
}



From daemon Sun Sep 22 12:25:12 2002



From: michelle-snyder-at-augustana.edu ()
Date: Sun, 22 Sep 2002 12:00:46 -0500
Subject: :Ask-A-Microscopist: Service Needed for a Sorvall MT-2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (michelle-snyder-at-augustana.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
September 22, 2002 at 10:04:10
---------------------------------------------------------------------------

Email: michelle-snyder-at-augustana.edu
Name: Michelle Snyder

Organization: St Francis Medical Center School of Clinical Laboratory Science

Education: Graduate College

Location: Peoria, Il, USA

Question: Do you have any idea who could service a Sorvall MT-2 ultra
microtome? I am experiencing macroscopic chatter, I am assuming
something is wrong with a bearing or the counterweight. I have
contacted serveral companies and each gives me another name with no
luck...Leica, Kendro, Ventana, Broeckler, etc. Any help would be
much appreciated, I just want to get back to sectioning. Thanks so
much!

---------------------------------------------------------------------------


From daemon Sun Sep 22 14:08:07 2002



From: Donald Werder :      aettinger1984-at-comcast.net
Date: Sun, 22 Sep 2002 14:59:41 -0400
Subject: Re: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Magnetic domains can also be imaged in a TEM using the technique of
Lorentz microscopy. In Lorentz microscopy you work at a very low or zero
objective lens excitation so as not to affect the domains. You will have
to adjust focus with the z-height if possible. You will also have a
scale problem.

You might be able to seen the domains in SEM if you decorate the domains
with very fine ferromagnetic particles,
a la the Bitter effect. There are also SEMs that have spin polarizing
filters that you could use to image the domains.

I believe the US intelligence agencies recommend overwriting and
defragmenting your disk seven times. They probably can extract remanent
domains that remain after incomplete eraser.

Don Werder

Allen Sampson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Sun Sep 22 17:49:16 2002



From: John Foust :      jfoust-at-threedee.com
Date: Sun, 22 Sep 2002 17:35:24 -0500
Subject: Re: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:05 PM 9/21/2002 -0400, simkin-at-egr.msu.edu"-at-sparc5.microscopy.com wrote:
} The person whom I received the question from is an old childhood friend with
} a strong curiosity. He is one of the computer geeks for a local programming
} concern.
} The thing that piqued his interest was an assertion that it required a
} minimum of 7 writing cycles to obscure old drive data, so he wants to test it.
} I confess myself surprised by this, so now I'm interested as well. I would
} not have expected much of anything to have survived more than 3 writes.
} I find myself wondering if (beyond track wander) there is less than perfect
} flipping of domains in the entire area of a bit.

If your friend did a little digging into the published descriptions
of the field of computer forensics, he would find a number of
explanations of the techniques and equipment used to recover
this phantom data. They don't use SEMs. The re-written data can
be recovered in some cases because the magnetized regions are
generally larger than the read heads, and ghosts of the past
data can be discerned if you examine the trackways with something
other than the ordinary read-head.

- John



From daemon Sun Sep 22 20:43:52 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Sun, 22 Sep 2002 21:35:33 -0400 (EDT)
Subject: RE: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you pursue this discussion any further, I suspect you will have
violated the new Patriot Act and numerous other laws forbidding
discussion of the possibility of maybe uncovering classified data.
-Michael



From daemon Sun Sep 22 22:38:50 2002



From: spyker-at-bright.net ()
Date: Sun, 22 Sep 2002 22:29:41 -0500
Subject: Ask-A-Microscopist:LM Insect Wings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (spyker-at-bright.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
September 22, 2002 at 15:45:54
---------------------------------------------------------------------------

Email: spyker-at-bright.net
Name: lauren

Organization: Perry Jr. High

Education: 6-8th Grade Middle School

Location: Lima, Ohio USA

Question: What is the best way to prepare an insect wing for a slide mount...

---------------------------------------------------------------------------


From daemon Mon Sep 23 01:44:48 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Sun, 22 Sep 2002 23:33:11 -0400
Subject: Re: back issues of JCB for the asking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 9/18/02 7:18 PM, Tobias Baskin at BaskinT-at-missouri.edu wrote:
}
}
} Microscopists,
} I have a run of Journal of Cell Biology from 1987 through
} 2001 that I would like to give away. Our library doesn't want them.
} Anyone out there interested? Or know of a connection to libraries
} somewhere that might be interested?
}
Dear Tobias,
The American Physical Society has a journal donation program, and the
International Center for Theoretical Physics in Trieste will often reimburse
the donor for shipping journals to underdeveloped nations. While this may
not be appropriate for JCB, there may be similar programs sponsored by
biologically-oriented professional societies.
Yours,
Bill Tivol



From daemon Mon Sep 23 01:44:49 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Sun, 22 Sep 2002 23:33:11 -0400
Subject: Re: SF6 safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 9/19/02 1:08 PM, Mick Thomas at mgt3-at-ccmr.cornell.edu wrote:
}
} I would be very grateful if someone could offer me advice on how to ensure
} that a microscopy room is set up to safely handle a leak of SF6.
}
} Thank you very much for your time and help.
}
} Sincerely,
}
Dear Mick,
SF6 is not, itself, toxic; however, it can displace air, and, since it
will settle on the floor, if someone loses consciousness from lack of
oxygen, that can be fatal. Furthermore, I was recently told that SF6 can
produce F when it decomposes due to, say, a fire. The specifications for
SF6 safety are to have an exhaust, preferably located near the floor, which
pumps room air directly to the outside--i.e., not into the air conditioning
air return, etc. I do not know the air flow requirements, but someone else
on the list may have that info.
Yours,
Bill Tivol



From daemon Mon Sep 23 04:08:40 2002



From: Dirk Kirch :      kirch-at-imm.rwth-aachen.de
Date: Mon, 23 Sep 2002 10:51:37 +0200
Subject: Carbon Glue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear SEM Users!

The carbon glue, which was placed at the Leo 1530 is gone!
Please whoever took it bring it back. I need ist for today.


Dirk
+++++++++++++++++++++++++++++++++++++++++

Dirk Kirch
Institut fuer Metallkunde und Metallphysik
RWTH Aachen
D-52056 Aachen
Germany

Phone : +49-241-8026861
Fax : +49-241-8022301
Internet: http://www.imm.rwth-aachen.de
E-Mail : kirch-at-imm.rwth-aachen.de

+++++++++++++++++++++++++++++++++++++++++


From daemon Mon Sep 23 06:41:00 2002



From: benada-at-biomed.cas.cz
Date: Mon, 23 Sep 2002 13:33:37 +0200
Subject: Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to thank to all who kindly response to my question about
cleaning the C2 apertures of CM12.
The list of responses can be seen on:
http://www.biomed.cas.cz/~benada/c2_aperture_cleaning.htm

Many thanks again. Oldrich

+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-41062399
Fax: +420-2-41062347
WEB: http://www.biomed.cas.cz/mbu/lem113/lem.htm






From daemon Mon Sep 23 07:34:27 2002



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Mon, 23 Sep 2002 09:28:27 -0300
Subject: Re: SEM film and waveform

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After years of using a waveform monitor (what it's called on JEOL scopes)
with film, I really missed it on the 5600, even for digital images.
Setting brightness and contrast consistently, particularly for critical areas
of the image, is much easier with this tool. I missed it so much, I decided
to write my own software waveform monitor. See:

http://www.mta.ca/~jehrman/software.htm

Notice: I have a (small) commercial interest here, in that I sell the WFM
(and a companion program -LSP- as a filament saturation aid), to augment my
rather meager salary here in the Great White North. Using both routinely
have given me more consistent and pleasing images, and longer filament life.

Both will work on any Windows based digital instrument, or any imaging
software (such as Photoshop) where there is an interest in seeing gray-scale
values in a waveform format. Contact me offline if you're interested.

Regards,

Jim Ehrman

Allen Sampson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm not going to argue the merits of digital vs. analog at this point.
} Gary's point about the histogram has caught my interest.
}
} Many SEM's offer the ability to view an analog 'waveform' (each
} manufacturer seems to call it by a different name). This is essentially an
} oscillographic display of the signal intensity. It is a dynamic display of
} each scan line's signal as opposed to the static histogram of most image
} capture systems which offer a representation of the sum of all scan lines.
} The difference is that an accomplished operator may adjust the brightness
} and contrast to a particular feature of interest, rather than the total
} frame. You could put a digital system in line mode and manually run the
} line position up and down to characterize the feature of interest, but with
} the waveform display you can see this with every frame, as well as the
} overall signal frame response.
}
} Just a thought.
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
} On Friday, September 20, 2002 7:48 PM, Gary Gaugler [SMTP:gary-at-gaugler.com]
} wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } The ability of film to record SEM or TEM data has to be compared
} } to the scan rate density of SEM (2K) and TEM (integrated) versus
} } that of the actual film's capabilities.
} }
} } A finer grain film in a SEM environment at 2K lpi is not going to
} } be all that impressive compared to a direct digital capture at
} } 4Kx4K. the other factor in favor of digital capture is realtime
} } evaluation of the histogram of the total image. One can optimize
} } contrast and brightness to collect a perfect image. Using film, this
} } is hit or miss at best.
} }
} } gary g.
} }
} }
} } At 03:07 PM 9/20/2002, you wrote:
} }
} } } Ann and others,
} } } I am glad that you have found the MSA Facility Management series
} useful.
} } } Unfortunately it may be held less often in the future. Due to the large
} } } number of annually repeating sessions that limit addition of new
} sessions,
} } } it looks like it will be relegated to every other year starting
} immediately
} } } although there is no guarantee that it will be continued in the future.
} At
} } } least there will be no support yearly so speakers will have to be
} gleaned
} } } from those already going to the meetings. This is usually the case for
} most
} } } anyway but has not been the case for all.
} } } We will try to limp along if we can find appropriate facilitators
} } } already going to the meeting and a room to hold the session. Hopefully
} we
} } } will still get funding for the audio-visual required.
} } } In any case let's be optimistic and plan on a session at M&M2003.
} With
} } } that, I have a few ideas as a topic but, since this is meant to be
} relative
} } } to all you lab managers out there, what would you like to have
} } } presented/discussed at the next session? Please let me know with
} } } suggestions for facilitators as well.
} } }
} } } Debby
} } }
} } }
} } }
} } } On 9/20/02 11:32 AM, "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} wrote:
} } }
} } } } Hi Debbie,
} } } }
} } } } Many moons ago (in the 80's) I regularly used Kodak Ektapan sheet
} film in
} } } } 4x5 carriers inserted into the Polaroid holder, and got good results.
} Tech
} } } } Pan sounds promising though.
} } } }
} } } } I can appreciate your desire to get good negatives, as I am
} struggling with
} } } } an intro SEM course in which my students are only exposed to digital
} } } imaging
} } } } (no pun intended). I feel I am short-changing them.
} } } }
} } } } Let us know what you learn!!
} } } }
} } } } And thanks again for organizing the MSA facility management series.
} } } }
} } } } cheers,
} } } } Ann
} } } }
} } } } ###################
} } } } Ann Hein Lehman
} } } } Assistant Director, EM Facility
} } } } Trinity College - LSC314
} } } } 300 Summit Street
} } } } Hartford CT 06106
} } } } v. 860-297-4289
} } } } f. 860-297-2538
} } } } e. ann.lehman-at-trincoll.edu
} } } } w. http://www.trincoll.edu/~alehman/
} } } } Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
} } } }
} }
} }
} }
} }

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Mon Sep 23 08:31:39 2002



From: Paula Allan-Wojtas :      AllanWojtasP-at-agr.gc.ca
Date: Mon, 23 Sep 2002 08:51:36 -0400
Subject: Call for Nominations - Food Structure and Functionality Award

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


CALL FOR NOMINATIONS
FOOD STRUCTURE AND FUNCTIONALITY DIVISION ( at AOCS) AWARD


This award honors outstanding lifetime performance and meritorious contributions to an area of interest to the Food Structure & Functionality Division of the American Oil Chemists' Society. The award will be presented during the 94th AOCS annual Meeting & Exp to be held in Kansas City, Missouri, May 4-7, 2003 and consists of $1,500USD to help defray costs incurred by the awardee in participating in the AOCS Annual Meeting & Expo, and also a crystal plaque.

Nominations should consist of a letter of nomination, supporting letters from at least three other scientists, and biographical information concerning the nominee. The biographical information must include a summary of the nominee's research accomplishments, a list of publication, degree(s) held (with the names of granting institutions) and positions(s) held during the nominee's professional career.

For further information on nomination procedures contact the Food Structure & Functionality Division Award Canvassing Committee Chairperson, Dr, Judy Arnold, USDA-ARS_RRC, 950 College Station Rd., P.O. Box 5677, Athens, GA 30604-5677, USA, Tel: 706 546 3515 Fax: 706 546 3068, e-mail: jarnold-at-ars.usda.gov. Completed nominations should be submitted before December 1, 2002.

¯---------------------------------------------------------------------------------------------------------

Paula Allan-Wojtas, Chair,
Food Structure and Functionality Forum - a division of AOCS.





Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-agr.gc.ca



From daemon Mon Sep 23 08:46:34 2002



From: gary.m.brown-at-exxonmobil.com
Date: Mon, 23 Sep 2002 08:39:38 -0500
Subject: JEOL 2010F

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John and Listers,

I, too, would be interested in information on JEOL 2010F TEM's in the US.

Thanks to all,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



From daemon Mon Sep 23 08:56:00 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 23 Sep 2002 09:55:53 -0400
Subject: RE: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No one said anything about "classified" data in this discussion other than
to refer to who may have an interest in this. Technology such as data
retrieval is practiced in numerous industries not the least of which is data
comm.

Mr. Aschcroft may inspect my hard drive at his leisure.

Peter Tomic

-----Original Message-----
} From: Michael Cammer [mailto:cammer-at-aecom.yu.edu]
Sent: Sunday, September 22, 2002 9:36 PM
To: Allen Sampson
Cc: '"simkin-at-egr.msu.edu"-at-sparc5.microscopy.com';
Microscopy-at-sparc5.microscopy.com


If you pursue this discussion any further, I suspect you will have
violated the new Patriot Act and numerous other laws forbidding
discussion of the possibility of maybe uncovering classified data.
-Michael



From daemon Mon Sep 23 09:07:37 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Mon, 23 Sep 2002 10:00:31 -0400
Subject: Carbon Glue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Ah, I didn't take it! :-)

Darrell
USA

"Dirk Kirch" {kirch-at-imm.rwth-aachen.de} on 09/23/2002 04:51:37 AM

To: Microscopy-at-sparc5.microscopy.com
cc:


Dear SEM Users!

The carbon glue, which was placed at the Leo 1530 is gone!
Please whoever took it bring it back. I need ist for today.


Dirk
+++++++++++++++++++++++++++++++++++++++++

Dirk Kirch
Institut fuer Metallkunde und Metallphysik
RWTH Aachen
D-52056 Aachen
Germany

Phone : +49-241-8026861
Fax : +49-241-8022301
Internet: http://www.imm.rwth-aachen.de
E-Mail : kirch-at-imm.rwth-aachen.de

+++++++++++++++++++++++++++++++++++++++++







From daemon Mon Sep 23 09:14:25 2002



From: Judy Arnold :      JArnold-at-saa.ars.usda.gov
Date: Mon, 23 Sep 2002 10:08:09 -0400
Subject: Re: Call for Nominations - Food Structure and Functionality

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please note that the date in the last sentence is incorrect. The nominations should be submitted by NOVEMBER 1, 2002.
Thanks,
Judy
} } } "Paula Allan-Wojtas" {AllanWojtasP-at-agr.gc.ca} 09/23/02 08:51AM } } }
CALL FOR NOMINATIONS
FOOD STRUCTURE AND FUNCTIONALITY DIVISION ( at AOCS) AWARD


This award honors outstanding lifetime performance and meritorious contributions to an area of interest to the Food Structure & Functionality Division of the American Oil Chemists' Society. The award will be presented during the 94th AOCS annual Meeting & Exp to be held in Kansas City, Missouri, May 4-7, 2003 and consists of $1,500USD to help defray costs incurred by the awardee in participating in the AOCS Annual Meeting & Expo, and also a crystal plaque.

Nominations should consist of a letter of nomination, supporting letters from at least three other scientists, and biographical information concerning the nominee. The biographical information must include a summary of the nominee's research accomplishments, a list of publication, degree(s) held (with the names of granting institutions) and positions(s) held during the nominee's professional career.

For further information on nomination procedures contact the Food Structure & Functionality Division Award Canvassing Committee Chairperson, Dr, Judy Arnold, USDA-ARS_RRC, 950 College Station Rd., P.O. Box 5677, Athens, GA 30604-5677, USA, Tel: 706 546 3515 Fax: 706 546 3068, e-mail: jarnold-at-ars.usda.gov. Completed nominations should be submitted before December 1, 2002.

?---------------------------------------------------------------------------------------------------------

Paula Allan-Wojtas, Chair,
Food Structure and Functionality Forum - a division of AOCS.





Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-agr.gc.ca


______________________________
Judy W. Arnold, Ph.D.
Research Microbiologist
USDA-ARS, Russell Research Center
950 College Station Road
Athens, GA 30605

Phone 706-546-3515
FAX 706-546-3068




From daemon Mon Sep 23 09:32:27 2002



From: Shanling Shi :      Shanling.Shi-at-unilever.com
Date: Mon, 23 Sep 2002 10:23:55 -0400
Subject: Polymerisation of Unicryl with Leica AFS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear list,

Does anyone has experience embedding and polymerisation of Unicryl with Leica
AFS? I am preparing sample for LM and EM immunolabeling. I embed sample with
Unicryl and cure the resin in Leica AFS at 4C for more than 4 days. The resin
still looks partially cured. I was wondering if it was UV intensity problem or
other problem. Any suggestion and references are appreciated!!! Thanks.

Shanling

Shanling Shi
Advanced Imaging & Measurement
Unilever Research US
45 River Road
Edgewater, NJ 07020
201-840-2340
Shanling.Shi-at-unilever.com



From daemon Mon Sep 23 09:45:13 2002



From: sstouden-at-thelinks.com
Date: Mon, 23 Sep 2002 09:37:59 -0500 (CDT)
Subject: RE: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



that is an interesting observation. which section of the patriot act are
you speaking of and which other laws (new or old) might be involved?
sterling


On Sun, 22 Sep 2002, Michael Cammer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} If you pursue this discussion any further, I suspect you will have
} violated the new Patriot Act and numerous other laws forbidding
} discussion of the possibility of maybe uncovering classified data.
} -Michael
}
}



From daemon Mon Sep 23 10:57:37 2002



From: Ayten Celik :      celik-at-students.uiuc.edu
Date: Mon, 23 Sep 2002 10:49:07 -0500 (CDT)
Subject: RE: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear all,

If anybody wants to make sure about the proper deletion of the data on
her/his hard disk, she/he may simply burn the hard disk...:)

Ayten.



From daemon Mon Sep 23 11:55:37 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 23 Sep 2002 09:47:14 -0700
Subject: Re: Ask-A-Microscopist:LM Insect Wings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Email: spyker-at-bright.net
} Name: lauren
}
} Organization: Perry Jr. High
}
} Education: 6-8th Grade Middle School
}
} Location: Lima, Ohio USA
}
} Question: What is the best way to prepare an insect wing for a slide mount...
}
} ---------------------------------------------------------------------------
Lauren -

Let's keep this simple. To avoid damage, you should use a cover
slip over the wing. You need some kind of a mounting medium under the
coverslip. Water is OK for a temporary mount, but for permanance, use
glycerin, clear nail polish, or "liquid bandage" from a drugstore. If you
use glycerin, which never dries, you'll need to seal the coverslip edges
with nailpolish.
An entomologist in Texas uses a fascinating approach to low
magnification study of dragonfly wings. He chills a live dragonfly in a
refrigerator, places it in his computer scanner, and makes a hi-res scan.
When it warms up, it can fly away. Directions and beautiful images are at
http://stephenville.tamu.edu/~fmitchel/dragonfly/index.html

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon Sep 23 13:47:56 2002



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Mon, 23 Sep 2002 15:36:22 -0300
Subject: Carbon Glue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hmm, I knew there must be a downside to this
newfangled "telepresence microscopy" thing!

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Ah, I didn't take it! :-)

Darrell
USA

"Dirk Kirch" {kirch-at-imm.rwth-aachen.de} on 09/23/2002 04:51:37 AM

To: Microscopy-at-sparc5.microscopy.com
cc:


Dear SEM Users!

The carbon glue, which was placed at the Leo 1530 is gone!
Please whoever took it bring it back. I need ist for today.


Dirk
+++++++++++++++++++++++++++++++++++++++++

Dirk Kirch
Institut fuer Metallkunde und Metallphysik
RWTH Aachen
D-52056 Aachen
Germany

Phone : +49-241-8026861
Fax : +49-241-8022301
Internet: http://www.imm.rwth-aachen.de
E-Mail : kirch-at-imm.rwth-aachen.de

+++++++++++++++++++++++++++++++++++++++++





From daemon Mon Sep 23 14:02:53 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Mon, 23 Sep 2002 14:56:43 -0400
Subject: RE: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, Dirk


It was me!

But I'm not bringing it back.

You have to come and get it!

cheers

rtch



} From: "Dirk Kirch" {kirch-at-imm.rwth-aachen.de}
To: Microscopy-at-sparc5.microscopy.com
Date sent: Mon, 23 Sep 2002 10:51:37 +0200


Ok, I admit it was a bad joke. Apologies.


} } If you pursue this discussion any further, I suspect you will have
} } violated the new Patriot Act and numerous other laws forbidding
} } discussion of the possibility of maybe uncovering classified data.
} } -Michael
} }
} }




From daemon Mon Sep 23 14:58:57 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Mon, 23 Sep 2002 15:49:08 -0700
Subject: reichert ultracut e

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



dear listers,
does anyone know the proper way to take the top off a reichert ultracut e
ultramicrotome?
tia,
beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Mon Sep 23 14:58:57 2002



From: John Mansfield :      jfmjfm-at-umich.edu
Date: Mon, 23 Sep 2002 15:51:11 -0400
Subject: DI AFM Software missing TIFF Export

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have had a Nanoscope AFM since about 1994 and one of the features the
students use in the software is the simple TIFF export function that
converts just the image data to a grayscale (or color) TIFF image.

We recently upgraded the software on both of our AFM systems to version
5.12r3. The TIFF export menu is still there but it doesn¹t let you export
just the image data, now you have to export the whole screen and then use
another image processing program to extract just the image data! In the
manual for this version it says that you can do just the image but there is
no option for it in the software.

I called DI and asked about this and the tech support guy didn¹t know why it
wasn't there and went to ask the programmers and here is the exact reply I
got:

"As far as the Tiff export goes, I talked to some of our software guys. They
tell me the File export for the TIFF never worked very well so they took it
out. But, the file can be cropped in PhotoShop."

Well, we had been using it for nearly 8 years, but I guess we are no judges
of how well it works! So they arbitrarily changed the functionality of the
software and didn¹t even both to change the manual or tell anyone! They
seem to be able to save the whole screen easily enough, but not the file
data, how weird is that?
Even I could program the saving of a simple TIFF file, but apparently this
is beyond the software engineers at DI. I wrote back and asked for an
explanation and was promptly ignored. So I thought this tirade to the net
community might wake up someone at DI.

Just so you know, these are my opinions and thoughts, however unreasoanable,
my employer has no connection to them whatsoever.


--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"




From daemon Mon Sep 23 15:19:40 2002



From: Heike Gabrisch :      hgabrisc-at-uno.edu
Date: Mon, 23 Sep 2002 15:16:36 -0500
Subject: Graduate Students/Materials Sciences/Chemistry/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Graduate students wanted for Materials Sciences and TEM work

The Information Technology Initiative of Louisiana supplies a grant for
graduate students to enter a 4 year PhD program at the University of New
Orleans.
Projects include characterization of transition metal oxides
(crystallography, lattice defect analysis) and in-situ heating experiments
of two phases alloys.

Contact :

Heike Gabrisch
Assistant Professor of Chemistry and Material Sciences
Department of Chemistry/AMRI
University of New Orleans
New Orleans, LA 70148

hgabrisc-at-uno.edu... phone (504 )280-1122 ... fax (504) 280-3185 ...






From daemon Tue Sep 24 00:55:31 2002



From: Allen Sampson :      ars-at-sem.com
Date: Tue, 24 Sep 2002 00:43:19 -0700
Subject: RE: DI AFM Software missing TIFF Export

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'd be happy to join this tirade. I met John a few years ago at UM -
between his rep in the field, his postings here and that meeting, I know
him to be a force in microscopy. As a programmer, I also know how right he
is in his outrage at such assertions from a manufacturer.

It's clear from what he is being told that the programmers at DI are simply
doing a screen dump to TIFF, using the image data written to the screen
object. This is unfortunate as the resolution will also be limited to that
of the screen. The TIFF file structure was intentionally designed to be
open and simple in order to be a widely used standard. A directory
contains the location of file elements, a number of 'tags' are written that
describe the file contents and the data is written to the file in a simple
format.

Ideally, when writing a TIFF file from a program like this, all acquired
data will be written to the file as a graphical representation that uses
the same resolution and data as that of the original scans. Programs used
later in the presentation can then adjust the image for whatever needs you
may have. The TIFF standards allow for easy methods to write this data to
file in a way that can be understood by any programs that also follow TIFF
standards. In our work, TIFF is severely limited in several ways, but is
currently the best method to transfer image data.

Scanning probe methods create data that is not generally depicted as it is.
The Z axis data, or intensity of a two dimensional image, is normally
translated, as well as the X and Y data, into a rotationally translated
image representing surface topography. Thus the data collected needs this
imaging translation for presentation.

Windows gives you various canvasses to draw image data on - the screen and
printer are two better known examples. When drawing to any of these
canvases, the first job is to determine their capabilities, i.e.
resolution, and adjust your drawing to those limitations. But you can also
create 'phantom' canvases that have arbitrary resolutions. These are
basically memory spaces that the image data is written to. Once they are
completed, you have a set of data that is appropriate for directly
exporting to TIFF format. This background operation can be completed in a
fraction of a second and a TIFF file created in a second or two, depending
on the size.

I hope this gives enough of a hint to the DI programmers on how to do it,
if not please feel free fire their asses or have them contact me. This is
an egregious situation for a company who's livelihood relies on computers.

Just so you know, these are my own opinions and thoughts, and I am my own
employer. I always call it like I see it, and what I see here stinks.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Monday, September 23, 2002 12:51 PM, John Mansfield [SMTP:jfmjfm-at-umic
h.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We have had a Nanoscope AFM since about 1994 and one of the features the
} students use in the software is the simple TIFF export function that
} converts just the image data to a grayscale (or color) TIFF image.
}
} We recently upgraded the software on both of our AFM systems to version
} 5.12r3. The TIFF export menu is still there but it doesn?t let you
export
} just the image data, now you have to export the whole screen and then use
} another image processing program to extract just the image data! In the
} manual for this version it says that you can do just the image but there
is
} no option for it in the software.
}
} I called DI and asked about this and the tech support guy didn?t know why
it
} wasn't there and went to ask the programmers and here is the exact reply
I
} got:
}
} "As far as the Tiff export goes, I talked to some of our software guys.
They
} tell me the File export for the TIFF never worked very well so they took
it
} out. But, the file can be cropped in PhotoShop."
}
} Well, we had been using it for nearly 8 years, but I guess we are no
judges
} of how well it works! So they arbitrarily changed the functionality of
the
} software and didn?t even both to change the manual or tell anyone! They
} seem to be able to save the whole screen easily enough, but not the file
} data, how weird is that?
} Even I could program the saving of a simple TIFF file, but apparently
this
} is beyond the software engineers at DI. I wrote back and asked for an
} explanation and was promptly ignored. So I thought this tirade to the
net
} community might wake up someone at DI.
}
} Just so you know, these are my opinions and thoughts, however
unreasoanable,
} my employer has no connection to them whatsoever.
}
}
} --
} John Mansfield PhD MInstP
} North Campus Electron Microbeam Analysis Laboratory
} 417 SRB, University of Michigan
} 2455 Hayward, Ann Arbor MI 48109-2143
} Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} (Leaving a phone message at 936-3352 is preferable to 834-3913)
} Email: jfmjfm-at-engin.umich.edu
} URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} Location: Lat. 42! 16' 48" Long. 83! 43' 48"
}
}
}
}
}



From daemon Tue Sep 24 07:34:45 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 24 Sep 2002 08:22:53 -0400
Subject: RE: (further) Hard Disk Microscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The rule of Law should be that if you start to read someone's hard drive,
you must do it bit-by-bit until you are bitten through!

Assuming that I understand the original question, which I now can't remember
- half the fun - here's a real look at data conservation by those who will,
in the end, have to be prepared for it, the Archaeologists.

http://www.ukoln.ac.uk/services/elib/papers/supporting/pdf/p2.pdf

Watch out, you'll need the Acrobat Reader!!!

Fred Monson
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Geology-Astronomy
West Chester University of Pennsylvania
Schmucker Science Center II
South Church street & Rosedale Avenue
West Chester, PA, 19383, USA
Phone: 610-738-0437
FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/
http://www.wcupa.edu/_visitors/


-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Monday, September 23, 2002 9:56 AM
To: 'Michael Cammer'; Allen Sampson
Cc: '"simkin-at-egr.msu.edu"-at-sparc5.microscopy.com';
Microscopy-at-sparc5.microscopy.com


No one said anything about "classified" data in this discussion other than
to refer to who may have an interest in this. Technology such as data
retrieval is practiced in numerous industries not the least of which is data
comm.

Mr. Aschcroft may inspect my hard drive at his leisure.

Peter Tomic

-----Original Message-----
} From: Michael Cammer [mailto:cammer-at-aecom.yu.edu]
Sent: Sunday, September 22, 2002 9:36 PM
To: Allen Sampson
Cc: '"simkin-at-egr.msu.edu"-at-sparc5.microscopy.com';
Microscopy-at-sparc5.microscopy.com


If you pursue this discussion any further, I suspect you will have
violated the new Patriot Act and numerous other laws forbidding
discussion of the possibility of maybe uncovering classified data.
-Michael



From daemon Tue Sep 24 07:34:46 2002



From: Rafal Dunin-Borkowski :      red10-at-cam.ac.uk
Date: Tue, 24 Sep 2002 13:20:05 +0100
Subject: TEM - Senior Technical Officer position in Cambridge from November

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Senior Technical Officer in Electron Microscopy:

The Department of Materials Science and Metallurgy at Cambridge has a
world-class suite of fifteen electron microscopes, both transmission and
scanning, and four electron microscope technicians.

Applications are invited for a Senior Technical Officer who will ensure
that the microscopes operate at peak performance and who will be
responsible for training users, both from within Cambridge University and
from outside, and interacting with microscope manufacturers. The post
holder will have a detailed knowledge of electron optics, vacuum systems,
etc., a background in physical science, and will be an expert user of
electron microscopes and their analytical attachments. It is not essential,
but it would be an advantage, if the person appointed could also give
graduate lectures in electron microscopy, be knowledgeable about image
simulation software and computing, develop new electron microscope
techniques and engage in research. However, the post is essentially a very
high level research support position.

This is a permanent position funded by the University of Cambridge. The
initial appointment will be for a period of five years, which may be
renewed until retirement. Salary will be in the range £22,522 to £32,537.

Further information about this position may be obtained by contacting

Dr Paul Midgley, Tel. +44 (0)1223 334561, E-mail: pam33-at-cam.ac.uk
or Professor Colin Humphreys, Tel. +44 (0)1223 334457, E-mail:
 colin.humphreys-at-msm.cam.ac.uk.

Applications, which should be in writing and include a CV and the names and
addresses of three referees, should be sent to:

Ms Lorraine Dann,
Departmental Administrator,
Department of Materials Science and Metallurgy,
University of Cambridge,
Pembroke Street,
Cambridge, CB2 3QZ,
UK.

Closing date: 1 Nov 2002.


From daemon Tue Sep 24 07:34:46 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Tue, 24 Sep 2002 14:22:12 +0200
Subject: HREM simulation / JEOL 3010

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I am wanting to do image simulation of HREM images from a JEOL 3010
(LaB6 filament). Does anyone know the spread of defocus due to
chromatic abberation for this microscope?

Also, I need to determine the semiangle of beam convergence. I assume
I just do this by photographing a convergent beam diffraction pattern
made using the same condensor aperture as for the image and calculating
the angle from the diffraction disk radius. Am I right?

Best wishes

--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Tue Sep 24 09:38:21 2002



From: slarocco-at-leica-microsystems.com
Date: Tue, 24 Sep 2002 09:24:29 -0500
Subject: Ultracut E cover removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Beth,

To remove the cover of an Ultracut E, there are a few things you must do.

1) Turn the stage course wheel located at the front of the machine counter
clockwise to move the stage towards you.

2) The remove the 2 large flat head screws located on the plate that
surrounds the stage. There will be on screw on each side. Completely
remove them.

3) Remove this plate but lifting it up and around the knife stage.

4) By removing that plate, it now makes visible two allen screws located on
each side of the microtome. The screws go through a bracket that holds
down the front of the cover. Completely remove these two screws, and
remove the brackets.

5) Lastly, you must remove the two allen key screws located at the rear of
the instrument towards the bottom on the backside of the cover. One these
are removed you are ready to remove the cover.

Things to check before removing the cover are:
Specimen arc and chuck should be removed
Spring loaded set screw that hold the specimen arc in place should be
removed completely
Handwheel needs to be removed (1 allen key screw located at the center
of the handwheel)
Remove knife holder from stage

Once all these steps are followed you should be able to remove the cover
completely. When placing the cover back onto the instrument, please be
sure to observe the serial port connection located at the back of the unit.
The male end of this plug is located on the cover and the female end is
located on the back portion of the microtome. You must be sure that when
placing the cover back on, that these two connections are plugged into each
other in order for the microtome to operate.

If you have any questions, please feel free to contact Leica's TAC Center
at 1-800-248-0123 Option 3, then Option 1.

Best Regards,
Shawn LaRocco
Manager, Service Administration and TAC
847.317.7263



From daemon Tue Sep 24 09:58:28 2002



From: gary.m.brown-at-exxonmobil.com
Date: Tue, 24 Sep 2002 09:49:43 -0500
Subject: Re: Recent Reply to Listserver Message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Bob,

Apparently part of the "microscopy-at-sparc5.microscopy.com" was missing.

What can I do for you?

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



"Bob Roberts"
{bobrobs-at-cox.ne To: {Gary.M.Brown-at-ExxonMobil.com}
t} cc:
Subject: Recent Reply to Listserver Message

09/23/02 08:29
PM





Hi Gary,

I just sent you a reply and it was addressed kinda weird. It had your above
email address but with "-at-sparc5microscopy.com" added to the end. Not sure
about how this happened. Any way if you didn't receive it, please let me
know.

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St
Topeka, Kansas 66617-1780
785.246.1232
www.emlabservices.com








From daemon Tue Sep 24 10:09:25 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Tue, 24 Sep 2002 10:55:12 -0400
Subject: Re: reichert ultracut e

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Beth,
Look where the head is attached to the body, you will see a screw with one
flat side, turn that
until the head can be slid off with the back/foward knob. That should do it
(you may have to turn
the head to the left).
Mike D

Beth Richardson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} dear listers,
} does anyone know the proper way to take the top off a reichert ultracut e
} ultramicrotome?
} tia,
} beth
}
} **********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} **********************************************************************
}
} "And it's only the giving that makes you what you are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
} ***************************************************************************



From daemon Tue Sep 24 10:19:05 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 24 Sep 2002 10:11:59 -0500
Subject: Re: reichert ultracut e

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Remove knife block and specimen block.
Back up the top piece with binoculars are far as it will go - you
need to gently push on it as you turn the knob. As it moves back,
you can see the plug where the upper lamp is plugged in. Unplug
this, and continue backing up the top until it comes off. Place it
somewhere safe - don't let the eyepieces fall out! You can remove
the whole binoc assembly first if you are worried.

Remove the metal rim that runs around the knife block. there are two
slotted screws at the rear of the plate - just in front of where the
holes for the little specimen key lives. Lift off the plate by
gently tilting the plate forward to get it off around the little
lever in front that locks the knife block in place.

once that plate is removed, you can see two L-shaped brackets that
are held down by allen screws that hold the front end of the top in
place. remove them.

Next remove the two allen screws at the back of the microtome near
the bottom of the top cover. You can now remove the top by gently
lifting up. Next, gasp at all the crud that has built up inside! I
have done this a bunch of times without a problem. Keyword = GENTLE.
Good luck, tom


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Sep 24 10:19:05 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Tue, 24 Sep 2002 11:10:26 -0700
Subject: thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for all the emails and phone calls about taking the top off the
Reichert!
I have the information that I need now.
This list is the best!
thanks again,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Tue Sep 24 10:58:49 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Tue, 24 Sep 2002 10:49:55 -0500
Subject: Jeol 733 X-Ray Maps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group

I have been having trouble collecting film based elemental X-ray maps on my Jeol 733 at a magnification of 40X. The map of a mineral grain that fills the screen at this mag (2.5mmx2.5mm?) only shows a concentration of dots diagonally near the center of the grain. I suspect I may be beyond the area in which I can collect X-rays or someting is misaligned. Any comments will be appreciated.

Thanks

Roy Beavers
Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, Tx. 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-mail.smu.edu



From daemon Tue Sep 24 11:51:44 2002



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Tue, 24 Sep 2002 11:37:31 -0500
Subject: RE: HREM simulation / JEOL 3010

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Ian,

The spec. for the JEOL 3010 gives Cc as polepiece dependent. The UHR
polepiece has Cc=1.3 mm and the HR has 1.8 mm.

The defocus spread is given as Cc*(delta(E)/E), roughly, where delta(E) is
the energy spread of the source. This assumes that the contribution due to
objective lens current fluctuation is negligible (see for example O.
Krivanek "Practical High Resolution Electron Microscopy" in a book edited by
Buseck et al, 1984 (Oxford)). Now delta(E) is dependent on how you run your
filament, but might be somewhere in the range of a couple of eV for a
thermionic gun. So a good estimate of defocus spread would be somewhere
about 100 Angstroms. I would be interested if anyone can provide a more
accurate value than this, or if I am seriously off here.

On the second point: You cannot always simply go to crossover and measure
the radius of your CBED disks in order to get the beam convergence for
estimating spatial coherence. Specifically, if you are doing HREM imaging
with a defocused beam on your sample, the act of defocusing is actually
increasing coherence locally. The angular spread of the beam at a single
point is a lot less than the total spread across the whole illuminated area.
On older TEM's one typically operated close to crossover (filament image on
sample, and angular spread at a point in the back focal plane) so it was
possible to use the diffraction pattern to estimate spatial coherence, as
described by a 1975 article by O'Keefe and Sanders (in Acta Cryst.). If on
the other hand you're operating with a spread beam you will see small bright
and dark field images in the BFP. Since you are imaging angles in the BFP
the occurrence of images is just saying that the incident angle varies
across the illuminated area. The coherence is determined by the uncertainty
in the angle at a single point in your HREM image.

So how do you measure convergence if you are not recording images at
crossover? Maybe you can decrease your condenser aperture as small as you
can go and still get comparable intensity, then measure the angular spread
in the BFP. Another approach might be to set up the conditions used for
imaging, then go to diffraction mode and re-focus the diffraction pattern
(decrease the intermediate lens current) to obtain sharp filament images,
then measure the half-width of the filament images relative to the absolute
scaling provided by the pattern. This will at any rate be better than using
the total angular range within the condenser aperture.

Regards,

Wharton Sinkler
UOP LLC
Des Plaines, IL 60017-5017
U. S. A.





} -----Original Message-----
} From: Ian MacLaren [SMTP:maclaren-at-tu-darmstadt.de]
} Sent: Tuesday, September 24, 2002 7:22 AM
} To: Microscopy
} Subject: HREM simulation / JEOL 3010
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} I am wanting to do image simulation of HREM images from a JEOL 3010
} (LaB6 filament). Does anyone know the spread of defocus due to
} chromatic abberation for this microscope?
}
} Also, I need to determine the semiangle of beam convergence. I assume
} I just do this by photographing a convergent beam diffraction pattern
} made using the same condensor aperture as for the image and calculating
} the angle from the diffraction disk radius. Am I right?
}
} Best wishes
}
} --
} Ian MacLaren
} Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
} ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/
}


From daemon Tue Sep 24 12:15:17 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 24 Sep 2002 10:03:52 -0700
Subject: Printers, again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

I missed MSA this year (fell off my bike, broke some things) so I didn't
get a chance to catch up on the latest and greatest regarding the current
state of the art for printing digital images etc.

Here's my dilema: We have a fancy dye sub printer, but it just broke and
needs repair. We have had it since 1996 and it got lots of use in the first
4 or 5 years, but business has fallen off lately, like down to one or two
uses in the last several months. I am trying to figure out what is
happening, my guess is that everyone is using some cheap new ink jets in
their own lab to do their printing. We are a small central services type
lab, our users visit us to use the instruments, then take their image files
with them. The dye sub used to get a lot of use from all kinds of people on
campus, but no longer.

So, what's up with printing? Do I get the dye sub fixed, or do I save the
$$ and buy a couple of photo ink jet things to do its job? There has been a
major transformation in imaging technologies here, our darkroom is
practically abandoned, the dye sub is mostly idle etc. Has printing become
a distributed resource where everyone does it independently, or have some
just given up on printing altogether and everything gets distributed and
submitted in electronic form?

Just curious.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Tue Sep 24 12:29:12 2002



From: David_Bell-at-millipore.com
Date: Tue, 24 Sep 2002 13:22:18 -0400
Subject: RE: DI AFM Software missing TIFF Export

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Allen,

I was totally with you right up until the point you chose to use
vulgarity, thereby losing any respect I had for your argument. It did not
make you look very professional, but of course, this is also my own
opinion and does not reflect that of my company. I will not comment
further on this thread other than to say that I, too, think DI could do a
better job of the tiff export function, especially where image analysis
may be performed on the images post export.

Sincerely,


David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108






Allen Sampson {ars-at-sem.com}
09/24/2002 03:43 AM
Please respond to "ars-at-sem.com"


To: "'John Mansfield'" {jfmjfm-at-umich.edu} , "microscopy-at-microscopy.com"
{microscopy-at-microscopy.com}
cc:
Subject: RE: DI AFM Software missing TIFF Export

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'd be happy to join this tirade. I met John a few years ago at UM -
between his rep in the field, his postings here and that meeting, I know
him to be a force in microscopy. As a programmer, I also know how right
he
is in his outrage at such assertions from a manufacturer.

It's clear from what he is being told that the programmers at DI are
simply
doing a screen dump to TIFF, using the image data written to the screen
object. This is unfortunate as the resolution will also be limited to
that
of the screen. The TIFF file structure was intentionally designed to be
open and simple in order to be a widely used standard. A directory
contains the location of file elements, a number of 'tags' are written
that
describe the file contents and the data is written to the file in a simple

format.

Ideally, when writing a TIFF file from a program like this, all acquired
data will be written to the file as a graphical representation that uses
the same resolution and data as that of the original scans. Programs used

later in the presentation can then adjust the image for whatever needs you

may have. The TIFF standards allow for easy methods to write this data to

file in a way that can be understood by any programs that also follow TIFF

standards. In our work, TIFF is severely limited in several ways, but is
currently the best method to transfer image data.

Scanning probe methods create data that is not generally depicted as it
is.
The Z axis data, or intensity of a two dimensional image, is normally
translated, as well as the X and Y data, into a rotationally translated
image representing surface topography. Thus the data collected needs this

imaging translation for presentation.

Windows gives you various canvasses to draw image data on - the screen and

printer are two better known examples. When drawing to any of these
canvases, the first job is to determine their capabilities, i.e.
resolution, and adjust your drawing to those limitations. But you can
also
create 'phantom' canvases that have arbitrary resolutions. These are
basically memory spaces that the image data is written to. Once they are
completed, you have a set of data that is appropriate for directly
exporting to TIFF format. This background operation can be completed in a

fraction of a second and a TIFF file created in a second or two, depending

on the size.

I hope this gives enough of a hint to the DI programmers on how to do it,
if not please feel free fire their asses or have them contact me. This is

an egregious situation for a company who's livelihood relies on computers.

Just so you know, these are my own opinions and thoughts, and I am my own
employer. I always call it like I see it, and what I see here stinks.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Monday, September 23, 2002 12:51 PM, John Mansfield [SMTP:jfmjfm-at-umic
h.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We have had a Nanoscope AFM since about 1994 and one of the features the
} students use in the software is the simple TIFF export function that
} converts just the image data to a grayscale (or color) TIFF image.
}
} We recently upgraded the software on both of our AFM systems to version
} 5.12r3. The TIFF export menu is still there but it doesn?t let you
export
} just the image data, now you have to export the whole screen and then
use
} another image processing program to extract just the image data! In the
} manual for this version it says that you can do just the image but there

is
} no option for it in the software.
}
} I called DI and asked about this and the tech support guy didn?t know
why
it
} wasn't there and went to ask the programmers and here is the exact reply

I
} got:
}
} "As far as the Tiff export goes, I talked to some of our software guys.
They
} tell me the File export for the TIFF never worked very well so they took

it
} out. But, the file can be cropped in PhotoShop."
}
} Well, we had been using it for nearly 8 years, but I guess we are no
judges
} of how well it works! So they arbitrarily changed the functionality of
the
} software and didn?t even both to change the manual or tell anyone! They
} seem to be able to save the whole screen easily enough, but not the file
} data, how weird is that?
} Even I could program the saving of a simple TIFF file, but apparently
this
} is beyond the software engineers at DI. I wrote back and asked for an
} explanation and was promptly ignored. So I thought this tirade to the
net
} community might wake up someone at DI.
}
} Just so you know, these are my opinions and thoughts, however
unreasoanable,
} my employer has no connection to them whatsoever.
}
}
} --
} John Mansfield PhD MInstP
} North Campus Electron Microbeam Analysis Laboratory
} 417 SRB, University of Michigan
} 2455 Hayward, Ann Arbor MI 48109-2143
} Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} (Leaving a phone message at 936-3352 is preferable to 834-3913)
} Email: jfmjfm-at-engin.umich.edu
} URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} Location: Lat. 42! 16' 48" Long. 83! 43' 48"
}
}
}
}
}







From daemon Tue Sep 24 13:16:38 2002



From: Stanley L. Flegler :      flegler-at-pilot.msu.edu
Date: Tue, 24 Sep 2002 14:04:08 -0400
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jonathan:

In reference to printers, we are a university core facility and serve a
broad spectrum of student and faculty users from 45 different departments.
We have laser jet, ink jet, dye sub, and silver halide digital printers.
The dye sub has received less and less use as the years have gone by. It
is a Tektronix Phaser 440 which at one time was the work horse of the
graphic arts industry. However, Tektronix completely dropped out of the
dye sub market over a year ago. In fact, we can no longer buy supplies for
it.

Most students now incorporate images directly into thesis text and print on
a laser jet or ink jet printer. More and more journals are accepting and
even preferring electronic submission. However, there are a substantial
number of users and journals that insist on photographic quality output.
These users have switched to our silver halide digital printer, a Fuji
Pictrography 3000. The output from this is a photo in the true sense of
the word because it uses silver halide technology similar to a photograph.
In my opinion, it is far better than we ever got from the dye sub printer.
Many professional photo labs are buying these for users that want the best
possible output.

This has been our experience. Hope it helps.

Stanley L. Flegler, Director
Center for Advanced Microscopy
Michigan State University


From daemon Tue Sep 24 13:30:35 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 24 Sep 2002 13:23:47 -0500
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think I would concur with your guesses. Everyone is probably doing it on
their own.

Current inkjet printers CAN do a quite good job rendering images on good
paper. I have heard John Mc Kenzie recommend them at his seminars. Granted
that many inkjets are still slow compared to other technologies. John even
suggests setting up a bank of relatively cheap inkjets to share the jobs
and keep through-put up. We haven't gone that route yet.

Users may also be settling for mediocre prints from their own printers not
knowing what quality is available. We have an aging Lexmark Optra Rt+ that
does a quite good job of printing B/W images on bright white paper. I just
passed some prints from it on to a user who had tried printing images on
his own printer. He much preferred our quality once he saw the prints. He
just assumed, falsely in this case, that he could get the same quality on
his own. Maybe you should post a classic image next to your printer so your
users see what you can do.

But having said that, I probably would consider retiring the printer
depending on the cost of repair. Printer technology has come a long way in
recent years and the new stuff generally does better for cheaper.

Warren

At 10:03 AM 9/24/02 -0700, you wrote:

} Hi:
}
} I missed MSA this year (fell off my bike, broke some things) so I didn't
} get a chance to catch up on the latest and greatest regarding the current
} state of the art for printing digital images etc.
}
} Here's my dilema: We have a fancy dye sub printer, but it just broke and
} needs repair. We have had it since 1996 and it got lots of use in the first
} 4 or 5 years, but business has fallen off lately, like down to one or two
} uses in the last several months. I am trying to figure out what is
} happening, my guess is that everyone is using some cheap new ink jets in
} their own lab to do their printing. We are a small central services type
} lab, our users visit us to use the instruments, then take their image files
} with them. The dye sub used to get a lot of use from all kinds of people on
} campus, but no longer.
}
} So, what's up with printing? Do I get the dye sub fixed, or do I save the
} $$ and buy a couple of photo ink jet things to do its job? There has been a
} major transformation in imaging technologies here, our darkroom is
} practically abandoned, the dye sub is mostly idle etc. Has printing become
} a distributed resource where everyone does it independently, or have some
} just given up on printing altogether and everything gets distributed and
} submitted in electronic form?
}
} Just curious.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Tue Sep 24 13:41:40 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 24 Sep 2002 13:34:37 -0500
Subject: Re: Jeol 733 X-Ray Maps

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It sure sounds like you are seeing the effect of the alignment of your
spectrometer. We used to have a WDS system on our SEM. We posted a couple
of images comparing WDS and EDS x-ray maps next to our scope. Two things
were quite apparent.

First, the better P/B ratio of WDS led to very low backgrounds and much
better contrast in the WDS maps compared to the EDS maps. Second, we saw
the intensity fall-off to the sides of the low-mag WDS maps due to
misalignment. The spectrometer was simply optimized for measuring
intensities at the center of the screen. By the time you moved the beam a
millimeter or two to the edge of the area, you screwed up your diffraction
conditions. We got a band of intensity that plainly showed us the position
of the spectrometer with respect to the raster and also the alignment of
the spectrometer (inclined or vertical).

You should be able to tweak your spectrometer settings by opening up the
slits, if I recall correctly, to reduce this effect. However, it will
probably still show more fall-off in intensity than does the EDS map.

Warren

At 10:49 AM 9/24/02 -0500, you wrote:
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-------------------------------------------
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-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Tue Sep 24 14:15:43 2002



From: S. Kuehner :      kuehner-at-u.washington.edu
Date: Tue, 24 Sep 2002 12:07:29 -0700 (PDT)
Subject: Re: Jeol 733 X-Ray Maps

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Roy-
What you are seeing in the Rowland Circle.... That is, the only part of
the beam raster that falls on the Rowland circle such that x-rays will be
diffracted. No amount of 'tweeking' will allow you to fill the screen
with x-rays at 40x if you are using WDS. For WDS x-ray maps the beam
raster must fall completely on the Rowland circle so magnifications of
greater then something like 1000x are needed. EDS can be used at low mags
as there is no Rowland circle requirement, though I always collect EDS
maps at the spectrometer focus (ie, sample is focused to the crosshairs in
reflected light). scott

************************************************
....amphiboles do violence to history...
T. Feininger, 2001. (taken out of context)
****************************

Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************

On Tue, 24 Sep 2002, Beavers, Roy wrote:

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}
} Group
}
} I have been having trouble collecting film based elemental X-ray maps on my Jeol 733 at a magnification of 40X. The map of a mineral grain that fills the screen at this mag (2.5mmx2.5mm?) only shows a concentration of dots diagonally near the center of the grain. I suspect I may be beyond the area in which I can collect X-rays or someting is misaligned. Any comments will be appreciated.
}
} Thanks
}
} Roy Beavers
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, Tx. 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-mail.smu.edu
}
}
}



From daemon Tue Sep 24 14:17:28 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Tue, 24 Sep 2002 15:12:05 -0400
Subject: Re: Printers, again

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Measure the time it takes to print an image of a Codonics 1600 (if you can
get it to work) and then measure the time it takes to print an image on an
Epson 980 or similar (the one's we happen to use) and then look at the cost
difference and see that I'd go for the Epson anytime. They're disposable.

Again, MY opinion, no one else's!

On 9/24/02 2:23 PM, "Warren E Straszheim" {wesaia-at-iastate.edu} wrote:

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}
}
} I think I would concur with your guesses. Everyone is probably doing it on
} their own.
}
} Current inkjet printers CAN do a quite good job rendering images on good
} paper. I have heard John Mc Kenzie recommend them at his seminars. Granted
} that many inkjets are still slow compared to other technologies. John even
} suggests setting up a bank of relatively cheap inkjets to share the jobs
} and keep through-put up. We haven't gone that route yet.
}
} Users may also be settling for mediocre prints from their own printers not
} knowing what quality is available. We have an aging Lexmark Optra Rt+ that
} does a quite good job of printing B/W images on bright white paper. I just
} passed some prints from it on to a user who had tried printing images on
} his own printer. He much preferred our quality once he saw the prints. He
} just assumed, falsely in this case, that he could get the same quality on
} his own. Maybe you should post a classic image next to your printer so your
} users see what you can do.
}
} But having said that, I probably would consider retiring the printer
} depending on the cost of repair. Printer technology has come a long way in
} recent years and the new stuff generally does better for cheaper.
}
} Warren
}
} At 10:03 AM 9/24/02 -0700, you wrote:
}
} } Hi:
} }
} } I missed MSA this year (fell off my bike, broke some things) so I didn't
} } get a chance to catch up on the latest and greatest regarding the current
} } state of the art for printing digital images etc.
} }
} } Here's my dilema: We have a fancy dye sub printer, but it just broke and
} } needs repair. We have had it since 1996 and it got lots of use in the first
} } 4 or 5 years, but business has fallen off lately, like down to one or two
} } uses in the last several months. I am trying to figure out what is
} } happening, my guess is that everyone is using some cheap new ink jets in
} } their own lab to do their printing. We are a small central services type
} } lab, our users visit us to use the instruments, then take their image files
} } with them. The dye sub used to get a lot of use from all kinds of people on
} } campus, but no longer.
} }
} } So, what's up with printing? Do I get the dye sub fixed, or do I save the
} } $$ and buy a couple of photo ink jet things to do its job? There has been a
} } major transformation in imaging technologies here, our darkroom is
} } practically abandoned, the dye sub is mostly idle etc. Has printing become
} } a distributed resource where everyone does it independently, or have some
} } just given up on printing altogether and everything gets distributed and
} } submitted in electronic form?
} }
} } Just curious.
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 23 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking
}
}
}
}

--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"




From daemon Tue Sep 24 15:35:52 2002



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Tue, 24 Sep 2002 16:25:13 -0400
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
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Jonathan,
Sorry about your bike encounter. Hope you're on the mend.
I'm sure you'll get lots of responses re your printer query. We are also a
central facility and also had a very expensive Kodak dye-sub that cost a
fortune to repair. After the second breakdown, we spent the money on an
Olympus dye-sub which is very nice and cost around $700. The only problem
is that it will print only 3 colors, unlike the Kodak which had
interchangeable ribbons for 4 color, 3 color, or black. The other printer
we have which was even less expensive is an Epson photo stylus which prints
nice images but they will fade in time. It is of course an ink jet and I
buy good paper, but they still fade. They are inexpensive and a good
source for "study prints" however.
I think a lot of users copy to a CD and print in their own labs as we too
have had a drop off in the printing business. Out of an average of 40
users a month, I have only one who still uses the darkroom.
Good luck with your printer decision.
Mary Gail Engle

At 10:03 AM 9/24/02 -0700, Jon Krupp wrote:
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Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-9700


From daemon Tue Sep 24 15:42:58 2002



From: Gib Ahlstrand :      ahlst007-at-tc.umn.edu
Date: Tue, 24 Sep 2002 15:43:19 -0500
Subject: Re: Jeol 733 X-Ray Maps

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Roy,

Sounds to me like your detector is probably not fully looking at the area of
sample you want to collect dot maps from. You probably have a collimator in
the nose of your x-ray detector which limits field of view.

Usually, just need to adjust working distance, perhaps sample tilt also -
toward detector - to bring area of interest onto axis, or line-of-sight, of
detector. Put in a clean aluminum stub, turn up mag to a few hundred times,
look at Al Ka x-rays count rate, and adjust working distance (keep image in
focus) until you see maximum count rate. then you have sample area dead
center on the axis of the EDS detector.

Also, because you are working at fairly low mag, only 40X, you may get some
collimator clipping anyway, but if axis is aligned as above, it should just
clip around the edges, circular-wise, like looking through a round hole.

For example, on my Hitachi S3500N, I need working distance of 16 mm (+ or -
1 mm) for optimum collection of x-rays.

Hope this helps!

Gib

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

"You can learn a lot by observation - just by lookin'!" - Yogi Bera

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Group
}
} I have been having trouble collecting film based elemental X-ray maps on my
} Jeol 733 at a magnification of 40X. The map of a mineral grain that fills the
} screen at this mag (2.5mmx2.5mm?) only shows a concentration of dots
} diagonally near the center of the grain. I suspect I may be beyond the area in
} which I can collect X-rays or someting is misaligned. Any comments will be
} appreciated.
}
} Thanks
}
} Roy Beavers
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, Tx. 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-mail.smu.edu
}
}
}

--




From daemon Tue Sep 24 18:17:16 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 24 Sep 2002 16:07:41 -0700
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
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My $.02.

Dye sub is pretty much out except for Kodak PS86xx. But each
print is rather pricey. Between the paper and the film, I figure
that each 8.5x11 print costs about $1.50.

Epson has mostly replaced dye sub with their photo ink jets.
Narrow size is the 890P and wide is the 9000P. They also
make a super wide (36" or so wide, virtually infinite length).
The quality on photo grade paper is awesome. But the speed
of printing is depressing. Be prepared to wait with a six pack
of your favorite beverage while printing an 11x17" print. But
the results are very good.

Tonal range is good. Durability of the print is good. Cost of
paper is good. The only down side is that the Epson photo
ink jets use a CMYK ink cartridge system and are very
proprietary (not refillable).... a tad pricey.

gary g.


At 10:03 AM 9/24/2002, you wrote:

} Hi:
}
} I missed MSA this year (fell off my bike, broke some things) so I didn't
} get a chance to catch up on the latest and greatest regarding the current
} state of the art for printing digital images etc.
}
} Here's my dilema: We have a fancy dye sub printer, but it just broke and
} needs repair. We have had it since 1996 and it got lots of use in the first
} 4 or 5 years, but business has fallen off lately, like down to one or two
} uses in the last several months. I am trying to figure out what is
} happening, my guess is that everyone is using some cheap new ink jets in
} their own lab to do their printing. We are a small central services type
} lab, our users visit us to use the instruments, then take their image files
} with them. The dye sub used to get a lot of use from all kinds of people on
} campus, but no longer.
}
} So, what's up with printing? Do I get the dye sub fixed, or do I save the
} $$ and buy a couple of photo ink jet things to do its job? There has been a
} major transformation in imaging technologies here, our darkroom is
} practically abandoned, the dye sub is mostly idle etc. Has printing become
} a distributed resource where everyone does it independently, or have some
} just given up on printing altogether and everything gets distributed and
} submitted in electronic form?
}
} Just curious.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu



From daemon Tue Sep 24 21:48:37 2002



From: Allen Sampson :      ars-at-sem.com
Date: Tue, 24 Sep 2002 21:43:30 -0700
Subject: RE: DI AFM Software missing TIFF Export

Contents Retrieved from Microscopy Listserver Archives
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Mea culpa.

It was late and it really grates when a good and innovative company begins
down this path. The vulgarity wasn't necessary or warranted. This is also
not a forum for the discussion of what, exactly, can be considered
vulgarity in this day and age.

My most humble apologies to any and all who may have taken offense at my
use of that one word. I promise to attempt to expand my meager vocabulary
in other directions in the future. I'm sorry if that word detracted in any
way from the other 493 I wrote.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Tuesday, September 24, 2002 10:22 AM,
"David_Bell-at-millipore.com"-at-sparc5.microscopy.com
[SMTP:"David_Bell-at-millipore.com"-at-sparc5.microscopy.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Allen,
}
} I was totally with you right up until the point you chose to use
} vulgarity, thereby losing any respect I had for your argument. It did
not
} make you look very professional, but of course, this is also my own
} opinion and does not reflect that of my company. I will not comment
} further on this thread other than to say that I, too, think DI could do a
} better job of the tiff export function, especially where image analysis
} may be performed on the images post export.
}
} Sincerely,
}
}
} David Bell
} Scientist
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108
}
}
}
}
}
}
} Allen Sampson {ars-at-sem.com}
} 09/24/2002 03:43 AM
} Please respond to "ars-at-sem.com"
}
}
} To: "'John Mansfield'" {jfmjfm-at-umich.edu} ,
"microscopy-at-microscopy.com"
} {microscopy-at-microscopy.com}
} cc:
} Subject: RE: DI AFM Software missing TIFF Export
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} I'd be happy to join this tirade. I met John a few years ago at UM -
} between his rep in the field, his postings here and that meeting, I know
} him to be a force in microscopy. As a programmer, I also know how right
} he
} is in his outrage at such assertions from a manufacturer.
}
} It's clear from what he is being told that the programmers at DI are
} simply
} doing a screen dump to TIFF, using the image data written to the screen
} object. This is unfortunate as the resolution will also be limited to
} that
} of the screen. The TIFF file structure was intentionally designed to be
} open and simple in order to be a widely used standard. A directory
} contains the location of file elements, a number of 'tags' are written
} that
} describe the file contents and the data is written to the file in a
simple
}
} format.
}
} Ideally, when writing a TIFF file from a program like this, all acquired
} data will be written to the file as a graphical representation that uses
} the same resolution and data as that of the original scans. Programs
used
}
} later in the presentation can then adjust the image for whatever needs
you
}
} may have. The TIFF standards allow for easy methods to write this data
to
}
} file in a way that can be understood by any programs that also follow
TIFF
}
} standards. In our work, TIFF is severely limited in several ways, but is
} currently the best method to transfer image data.
}
} Scanning probe methods create data that is not generally depicted as it
} is.
} The Z axis data, or intensity of a two dimensional image, is normally
} translated, as well as the X and Y data, into a rotationally translated
} image representing surface topography. Thus the data collected needs
this
}
} imaging translation for presentation.
}
} Windows gives you various canvasses to draw image data on - the screen
and
}
} printer are two better known examples. When drawing to any of these
} canvases, the first job is to determine their capabilities, i.e.
} resolution, and adjust your drawing to those limitations. But you can
} also
} create 'phantom' canvases that have arbitrary resolutions. These are
} basically memory spaces that the image data is written to. Once they are
} completed, you have a set of data that is appropriate for directly
} exporting to TIFF format. This background operation can be completed in
a
}
} fraction of a second and a TIFF file created in a second or two,
depending
}
} on the size.
}
} I hope this gives enough of a hint to the DI programmers on how to do it,
} if not please feel free fire their asses or have them contact me. This
is
}
} an egregious situation for a company who's livelihood relies on
computers.
}
} Just so you know, these are my own opinions and thoughts, and I am my own
} employer. I always call it like I see it, and what I see here stinks.
}
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} On Monday, September 23, 2002 12:51 PM, John Mansfield [SMTP:jfmjfm-at-umic
} h.edu] wrote:
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
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} }
-----------------------------------------------------------------------.
} }
} }
} } We have had a Nanoscope AFM since about 1994 and one of the features
the
} } students use in the software is the simple TIFF export function that
} } converts just the image data to a grayscale (or color) TIFF image.
} }
} } We recently upgraded the software on both of our AFM systems to version
} } 5.12r3. The TIFF export menu is still there but it doesn?t let you
} export
} } just the image data, now you have to export the whole screen and then
} use
} } another image processing program to extract just the image data! In
the
} } manual for this version it says that you can do just the image but
there
}
} is
} } no option for it in the software.
} }
} } I called DI and asked about this and the tech support guy didn?t know
} why
} it
} } wasn't there and went to ask the programmers and here is the exact
reply
}
} I
} } got:
} }
} } "As far as the Tiff export goes, I talked to some of our software guys.
} They
} } tell me the File export for the TIFF never worked very well so they
took
}
} it
} } out. But, the file can be cropped in PhotoShop."
} }
} } Well, we had been using it for nearly 8 years, but I guess we are no
} judges
} } of how well it works! So they arbitrarily changed the functionality of
} the
} } software and didn?t even both to change the manual or tell anyone!
They
} } seem to be able to save the whole screen easily enough, but not the
file
} } data, how weird is that?
} } Even I could program the saving of a simple TIFF file, but apparently
} this
} } is beyond the software engineers at DI. I wrote back and asked for an
} } explanation and was promptly ignored. So I thought this tirade to the
} net
} } community might wake up someone at DI.
} }
} } Just so you know, these are my opinions and thoughts, however
} unreasoanable,
} } my employer has no connection to them whatsoever.
} }
} }
} } --
} } John Mansfield PhD MInstP
} } North Campus Electron Microbeam Analysis Laboratory
} } 417 SRB, University of Michigan
} } 2455 Hayward, Ann Arbor MI 48109-2143
} } Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} } (Leaving a phone message at 936-3352 is preferable to 834-3913)
} } Email: jfmjfm-at-engin.umich.edu
} } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} } Location: Lat. 42! 16' 48" Long. 83! 43' 48"
} }
} }
} }
} }
} }
}
}
}
}
}
}
}
}



From daemon Tue Sep 24 21:52:49 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 24 Sep 2002 19:47:38 -0700
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
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I was thinking about printers another day. We do have sort of central
facility with super-duper Tektronix dye-sub and
color-laser. Dye-sub prints tends to fade (controversy to the previous
posting) and our particular printer is slow. Plus $3+ cost for each
print... Keeping in mind that most publishers accept now digital format...
For myself I decided to use laser-color (somehow it works better than B&W
laser) for drafts (quick and very reasonable quality, plus CHEAP) and ink
jet for near-photo quality prints. Right now I am setting up Epson 890
photo printer. I am going to use archival quality photo paper (more than
20 years image life) and 'gray-ink' Lyson cartridges. "Gray-ink" supposed
to deliver outstanding image quality (if did not kill printer's head) and
extended image life. My current situation is that Epson printer I've
received a few days ago is defective and will be replaced soon (I
hope). I'll report (if anybody interesting) my progress with
'gray-inks'. Sergey

At 12:12 PM 9/24/02, you wrote:
} ------------------------------------------------------------------------
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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Sep 24 22:14:10 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Wed, 25 Sep 2002 16:11:55 +1000
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
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Oh, come on!

That's not vulgarity!

Vulgarity is %#&(*$-at-&&$!!!!, and &*(^&*$%(&*($.

And how would vulgarity weaken the force of his argument, anyway?

Let's not be toooooo precious.

cheers

rtch





} From: "David_Bell-at-millipore.com"-at-sparc5.microscopy.com
To: "ars-at-sem.com" {ars-at-sem.com}
Copies to: jfmjfm-at-umich.edu, microscopy-at-microscopy.com


Dear Jonathon,

Since most of our images are collected digitally, we too have just
refurbished our large darkroom into a fluorescence microscopy lab. Yes,
lots of our users print out images on their own computers, and we have
several inkjets for what I'd call "rough" prints, plus a videoprinter on
the SEM, but for "good" printing, we use a central dye-sub printer, and
only for those journals that still require prints to copy from or that
specify that inkjet prints won't be accepted. So many journals now insist
on electronic submission of everything so it seems that producing a
stunning print isn't required for this any more. A pity, because some high
profile journals are publishing some pretty mediocre quality images, and
occasionally publish appalling images - pixellated, odd colours, etc.

my 2c (US1c) worth

}
} Hi:
}
} I missed MSA this year (fell off my bike, broke some things) so I didn't
} get a chance to catch up on the latest and greatest regarding the current
} state of the art for printing digital images etc.
}
} Here's my dilema: We have a fancy dye sub printer, but it just broke and
} needs repair. We have had it since 1996 and it got lots of use in the first
} 4 or 5 years, but business has fallen off lately, like down to one or two
} uses in the last several months. I am trying to figure out what is
} happening, my guess is that everyone is using some cheap new ink jets in
} their own lab to do their printing. We are a small central services type
} lab, our users visit us to use the instruments, then take their image files
} with them. The dye sub used to get a lot of use from all kinds of people on
} campus, but no longer.
}
} So, what's up with printing? Do I get the dye sub fixed, or do I save the
} $$ and buy a couple of photo ink jet things to do its job? There has been a
} major transformation in imaging technologies here, our darkroom is
} practically abandoned, the dye sub is mostly idle etc. Has printing become
} a distributed resource where everyone does it independently, or have some
} just given up on printing altogether and everything gets distributed and
} submitted in electronic form?
}
} Just curious.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu


Rosemary White rosemary.white-at-csiro.au
Microscopy Centre fax 61- 2 6246 5000
CSIRO Plant Industry ph. 61- 2 6246 5475 or
GPO Box 1600 mob. 61- 0402 835 973
Canberra, ACT 2601, Australia




From daemon Wed Sep 25 02:05:17 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Wed, 25 Sep 2002 08:54:35 +0200
Subject: RE: Printers, again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jonathan

We are in the process of setting up a new lab and "inherited" some printers
with some of the equipment. I was also involved in a extensive printer
comparison some time ago. My experience is that for normal cheap BW prints
the laser 1200 dpi works fine and is fast. On the other hand the Tektronix
Phaser does a good job both with colour and BW prints. With the black wax
blocs for free it is by far the cheapest prints for BW since it cost only
the paper. The images are sensitive to high temperatures e.g.. you can not
laminate them! Fading does not appear to be a problem. Personally I am not
a inkjet fan. The cartridges are expensive and to get really good images
special paper is needed. The dye sub is just to expensive for us here in
Botswana.

These are my views only.


Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gaborone
Botswana

Phone : +267 355 2426/5222
Mobile : +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw



} -----Original Message-----
} From: Stanley L. Flegler [mailto:flegler-at-pilot.msu.edu]
} Sent: Tuesday, September 24, 2002 8:04 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Printers, again
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Jonathan:
}
} In reference to printers, we are a university core facility
} and serve a
} broad spectrum of student and faculty users from 45 different
} departments.
} We have laser jet, ink jet, dye sub, and silver halide
} digital printers.
} The dye sub has received less and less use as the years have
} gone by. It
} is a Tektronix Phaser 440 which at one time was the work horse of the
} graphic arts industry. However, Tektronix completely dropped
} out of the
} dye sub market over a year ago. In fact, we can no longer
} buy supplies for
} it.
}
} Most students now incorporate images directly into thesis
} text and print on
} a laser jet or ink jet printer. More and more journals are
} accepting and
} even preferring electronic submission. However, there are a
} substantial
} number of users and journals that insist on photographic
} quality output.
} These users have switched to our silver halide digital printer, a Fuji
} Pictrography 3000. The output from this is a photo in the
} true sense of
} the word because it uses silver halide technology similar to
} a photograph.
} In my opinion, it is far better than we ever got from the dye
} sub printer.
} Many professional photo labs are buying these for users that
} want the best
} possible output.
}
} This has been our experience. Hope it helps.
}
} Stanley L. Flegler, Director
} Center for Advanced Microscopy
} Michigan State University
}


From daemon Wed Sep 25 02:38:00 2002



From: Frank Eggert :      Eggert-at-mikroanalytik.de
Date: Wed, 25 Sep 2002 09:26:12 +0200
Subject: Re: Jeol 733 X-Ray Maps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Roy,

using a WDX spectrometer you will have the problem with hudge geometry factors due to focussing principles with Rowland circle. The absolute detector efficiency depends very strong from the surface point, exciting from the electron beam. The differences are higher with lover magnifications, because the covered area is larger.
You are able to make X-ray maps with WDX and lower magnification only using stage control scanning! In this case, the absolute efficiency of the WDX spectrometer does not have any changes (everytimes the same geometry for the each point)

Using an EDX spectrometer the efficiency changing is some orders of magnitude lower than with WDX . But with low magnifications low gradients of intensity changings in the maps are visible, too (no changing of element concentration). The effect is higher with very short distances between specimen and detector. There are two ways to avoid the gradient:
- retract the X-ray spectrometer to higher specimen - detector distances (of course count rates are going down)
- you make a additional map setting one window to element freee bremsstrahlung (background) region. After the mapping
you have to process all element maps with special program. You have to divide all images through the bremsstrahlung
map. The resulting maps are free from geometrical effects but are a little bit more noisy (depends from measuring time for
all maps).


Dear Roy,
using a WDX spectrometer you will have the problem with huge geometry factors due to focus principles of Rowland circle. The absolute detector efficiency depends very strong from the surface point, which was excited from the electron beam. The differences are higher with lower magnifications, because the covered area is larger.
Only using stage control scanning it is possible to make X-ray maps with WDX with lower magnification! In this case, the absolute efficiency of the WDX spectrometer does not have any changes (every times the same geometry for each point).

Using an EDX spectrometer the efficiency changes are lower than with WDX . But with low magnifications low gradients of intensity in the maps are visible, too (no changing due to element concentration). The effect is higher with very short distances between specimen and detector. There are two ways to avoid the gradient:
- Retract the EDX X-ray spectrometer to higher distances between specimen and detector (of course count rates are going down)
- You take an additional map of an energy region of bremsstrahlung background (background free of element lines). After the mapping you have to process all element maps with special program. You have to divide (!) all images through the bremsstrahlung image. The resulting maps are free from geometrical effects but are a little bit more noisy (measuring time depending for all maps).

I hope, these hints are useful for you.

Frank Eggert

"Beavers, Roy" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Group
}
} I have been having trouble collecting film based elemental X-ray maps on my Jeol 733 at a magnification of 40X. The map of a mineral grain that fills the screen at this mag (2.5mmx2.5mm?) only shows a concentration of dots diagonally near the center of the grain. I suspect I may be beyond the area in which I can collect X-rays or someting is misaligned. Any comments will be appreciated.
}
} Thanks
}
} Roy Beavers
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, Tx. 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-mail.smu.edu



From daemon Wed Sep 25 02:59:18 2002



From: Frank Eggert :      Eggert-at-mikroanalytik.de
Date: Wed, 25 Sep 2002 09:49:03 +0200
Subject: Re: Jeol 733 X-Ray Maps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


.. Sorry, my first mail contains the text twice, a funny first version and the final text. Now the final text only:
----------------------------------------------------------------------------------------------------------

Dear Roy,
using a WDX spectrometer you will have the problem with huge geometry factors due to focus principles of Rowland circle. The absolute detector efficiency depends very strong from the surface point, which was excited from the electron beam. The differences are higher with lower magnifications, because the covered area is larger.
Only using stage control scanning it is possible to make X-ray maps with WDX with lower magnification! In this case, the absolute efficiency of the WDX spectrometer does not have any changes (every times the same geometry for each point).

Using an EDX spectrometer the efficiency changes are lower than with WDX . But with low magnifications low gradients of intensity in the maps are visible, too (no changing due to element concentration). The effect is higher with very short distances between specimen and detector. There are two ways to avoid the gradient:
- Retract the EDX X-ray spectrometer to higher distances between specimen and detector (of course count rates are going down)
- You take an additional map of an energy region of bremsstrahlung background (background free of element lines). After the mapping you have to process all element maps with special program. You have to divide (!) all images through the bremsstrahlung image. The resulting maps are free from geometrical effects but are a little bit more noisy (measuring time depending for all maps).

I hope, these hints are useful for you.

Frank Eggert

"Beavers, Roy" wrote:



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Group
}
} I have been having trouble collecting film based elemental X-ray maps on my Jeol 733 at a magnification of 40X. The map of a mineral grain that fills the screen at this mag (2.5mmx2.5mm?) only shows a concentration of dots diagonally near the center of the grain. I suspect I may be beyond the area in which I can collect X-rays or someting is misaligned. Any comments will be appreciated.
}
} Thanks
}
} Roy Beavers
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, Tx. 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-mail.smu.edu



From daemon Wed Sep 25 04:13:45 2002



From: Tony Bruton :      Bruton-at-nu.ac.za
Date: Wed, 25 Sep 2002 11:05:09 +0200
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rosemary has highlighted a point which has been of great concern to me
as a manager of an EM lab in the dawning (dawned !) digital era. With
the distribution of images electronically, locally or to journals afar,
we no longer have the same level of final, critical, control of the
quality of the image. I guess that there is no ready solution just the
passing of an age - though it is perhaps dubious that we had that much
'control' over the final rendering anyway !!

Tony

Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
Mob. 082 782 9640
website: www.nu.ac.za/microscopy.asp
Email: bruton-at-nu.ac.za
Postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa

} } } Rosemary White {rosemary.white-at-csiro.au} 09/25/02 08:11AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America


Dear Jonathon,

Since most of our images are collected digitally, we too have just
refurbished our large darkroom into a fluorescence microscopy lab.
Yes,
lots of our users print out images on their own computers, and we have
several inkjets for what I'd call "rough" prints, plus a videoprinter
on
the SEM, but for "good" printing, we use a central dye-sub printer,
and
only for those journals that still require prints to copy from or that
specify that inkjet prints won't be accepted. So many journals now
insist
on electronic submission of everything so it seems that producing a
stunning print isn't required for this any more. A pity, because some
high
profile journals are publishing some pretty mediocre quality images,
and
occasionally publish appalling images - pixellated, odd colours, etc.

my 2c (US1c) worth

}
} Hi:
}
} I missed MSA this year (fell off my bike, broke some things) so I
didn't
} get a chance to catch up on the latest and greatest regarding the
current
} state of the art for printing digital images etc.
}
} Here's my dilema: We have a fancy dye sub printer, but it just broke
and
} needs repair. We have had it since 1996 and it got lots of use in the
first
} 4 or 5 years, but business has fallen off lately, like down to one or
two
} uses in the last several months. I am trying to figure out what is
} happening, my guess is that everyone is using some cheap new ink jets
in
} their own lab to do their printing. We are a small central services
type
} lab, our users visit us to use the instruments, then take their image
files
} with them. The dye sub used to get a lot of use from all kinds of
people on
} campus, but no longer.
}
} So, what's up with printing? Do I get the dye sub fixed, or do I save
the
} $$ and buy a couple of photo ink jet things to do its job? There has
been a
} major transformation in imaging technologies here, our darkroom is
} practically abandoned, the dye sub is mostly idle etc. Has printing
become
} a distributed resource where everyone does it independently, or have
some
} just given up on printing altogether and everything gets distributed
and
} submitted in electronic form?
}
} Just curious.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu


Rosemary White rosemary.white-at-csiro.au
Microscopy Centre fax 61- 2 6246 5000
CSIRO Plant Industry ph. 61- 2 6246 5475 or
GPO Box 1600 mob. 61- 0402 835 973
Canberra, ACT 2601, Australia






From daemon Wed Sep 25 07:46:52 2002



From: =?iso-8859-1?Q?S=F8rensen_Henning_Sund?= :      Henning.S-at-danfoss.com
Date: Wed, 25 Sep 2002 14:33:56 +0200
Subject: doped epoxy resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I am currently investigating the properties of a permanent magnetic
material.
I need to distinguish between Nd-Fe-B particles, epoxy binder and porosities
on BSE images obtained on metallographic sections. No problem in singling
out the Nd-Fe-B, but do any of you have a suggestion of how to pick up the
porosities?

What we have tried up till now is to fill out the sectioned pores with a mix
of epoxy and fine silicon powder followed by repolishing to the same level.
No succes - the particles were too coarse.
I vaguely remember that I have heard about "doped epoxy resins" for this
kind of purpose. Perhaps some of you know a recipe or supplier.

Thank you,

Henning Sund Sørensen
Materials- and Process Consultant
Technology Centre
Danfoss A/S
6430 Nordborg, Denmark


From daemon Wed Sep 25 08:13:02 2002



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 25 Sep 2002 09:03:20 -0400
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergei, et al,

We have the PiezographyBW gray scale mods for an Epson 860 printer. This
replaces the color inkjet cartridges with gray scale cartridges. As part
of the package they provide a PhotoShop plugin to drive the printer (rather
than the default Epson "gray" scale driver). On Photo quality paper, the
prints are outstanding--fantastic gray scale discrimination, no
pixelation! Using a 10X loupe, I can't see any pixelation of the
image! The inks are pigments rather than dyes, so they are of archival
quality (i.e. won't fade)

It isn't real fast, but for {$400US, we got the printer and the Piezography
kit.

Just a satisfied customer...

Henk Colijn


At 07:47 PM 9/24/2002 -0700, Sergey Ryazantsev wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Wed Sep 25 09:02:46 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 25 Sep 2002 08:55:05 -0500
Subject: Re: doped epoxy resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the early 1980s, I was looking for a doped epoxy to provide contrast in
backscattered imaging between epoxy and coal. There was often a very slight
contrast, but we needed something more marked to facilitate image analysis.

I came across papers that suggested dissolving bromoform or iodaform in
epoxy resin to raise the atomic number. We chose iodaform. I think the
recipe was about 15% iodaform by weight in epoxy resin. (I think 18%
produced a saturated solution. Certainly less than 15% could be used.) We
warmed the mixture to aid dissolution. I could then store and use the resin
more or less as normal. I may have had to adjust (decrease) the amount of
hardener, but I think we may have also changed epoxy formulations about the
same time from a 4:1 mix to an 8:1 mix. Otherwise, the material behaved the
same.

Be advised that iodaform comes with plenty of health warnings. Be sure to
read and heed the advisories.

Warren Straszheim

with At 02:33 PM 9/25/02 +0200, you wrote:

} Dear Listers,
}
} I am currently investigating the properties of a permanent magnetic
} material.
} I need to distinguish between Nd-Fe-B particles, epoxy binder and porosities
} on BSE images obtained on metallographic sections. No problem in singling
} out the Nd-Fe-B, but do any of you have a suggestion of how to pick up the
} porosities?
}
} What we have tried up till now is to fill out the sectioned pores with a mix
} of epoxy and fine silicon powder followed by repolishing to the same level.
} No succes - the particles were too coarse.
} I vaguely remember that I have heard about "doped epoxy resins" for this
} kind of purpose. Perhaps some of you know a recipe or supplier.
}
} Thank you,
}
} Henning Sund Sørensen
} Materials- and Process Consultant
} Technology Centre
} Danfoss A/S
} 6430 Nordborg, Denmark




From daemon Wed Sep 25 09:07:56 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 25 Sep 2002 09:02:01 -0500
Subject: printers again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List,
From the single user's perspective, I'd like to put in a plug
for inkjets. I have an Epson 970. I am AMAZED at how well this thing
prints. I keep three grades of paper on hand, two are fancier types
of basic zerox paper, and the third is photopaper. The printer has
controls to adjust the quality and match to the paper. The fancier
white papers are not expensive (at least in USA) and the output on
them is wonderful and perfect for reviewer figures, grant proposals
(14 copies!), and handles color or black and white with no trouble.
And when I put in photopaper the output is phenomenal. I have never
even for a microsec considered using the fancy printers in our core
facility. For my own needs, the cartridges last long enough.

No financial ties to any printer co (or to anything else for
that matter, 8-).

Tobias Baskin
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Wed Sep 25 09:57:41 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 25 Sep 2002 10:55:50 -0400
Subject: Jeol 733 X-Ray Maps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Roy;

There's a few things I would check.

1. Do you have the energy of interest properly selected for mapping? That
is, selecting the energy at which the analyzer will place a dot on the map.
This is a software selection.

2. Are your counts/sec. high enough?

3. Is your beam energy high enough for excitation of the spectral line you
are trying to map?

4. Is the EDX detector's "field of view" catching the x-ray photons. This
will be some solid angle which is a function of the detector area, probe
angle etc.

And just a suggestion but put the SEM in a "spot mode" and see if you detect
the element of interest above the background noise. Are you mapping
multiple elements simultaneously or just one? What do you expect the
elemental concentration to be? Finally, what is the element and in what
elemental matrix is it in?

Peter

-----Original Message-----
} From: Beavers, Roy [mailto:rbeavers-at-post.cis.smu.edu]
Sent: Tuesday, September 24, 2002 11:50 AM
To: Microscopy Listserver; Microprobe List (E-mail)


Group

I have been having trouble collecting film based elemental X-ray maps on my
Jeol 733 at a magnification of 40X. The map of a mineral grain that fills
the screen at this mag (2.5mmx2.5mm?) only shows a concentration of dots
diagonally near the center of the grain. I suspect I may be beyond the area
in which I can collect X-rays or someting is misaligned. Any comments will
be appreciated.

Thanks

Roy Beavers
Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, Tx. 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-mail.smu.edu



From daemon Wed Sep 25 10:16:52 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 25 Sep 2002 10:09:01 -0500
Subject: TEM/LM: GMA Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I'm curious about any experiences people may have had with using glycol methacrylate, or similar resins, for TEM of biological tissue. Specifically, we are concerned with retinal tissue. We have found that we have seen flatter and more consistently stained thick sections from GMA embedded retina from another lab. We'd like to know if the quality of the EM work done with resin is good enough to warrant switching over from our standard EPON, EPON/Spurr's, or EPON/Araldite protocols, in order to get better quality LM of thicks.

My own opinion is that if TEM is part of the procedure, we should optimize the TEM protocols, even if the thicks don't look quite as nice. Running two protocols, one for LM and one for TEM, is not an option for us in this case.

We will run some tests, but any opinions or experiences are very welcome.

Thanks a bunch.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Wed Sep 25 10:17:28 2002



From: Jan Rutkowski :      zerutkow-at-cyf-kr.edu.pl
Date: Wed, 25 Sep 2002 17:22:31 +0200
Subject: SEM of paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,
We would like to make some experiments on analysis of old paper samples
serving as a support for oil painting. I would be grateful for any
information concerning the sample preparation for SEM (we have low vacuum
Jeol 5500 LV)

Thank you

Jan

dr. Jan Rutkowski
Academy of Fine Arts
Conservation Dept, Physics Lab
ul. Smolensk 9
31-108 Krakow, Poland




From daemon Wed Sep 25 11:04:23 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Wed, 25 Sep 2002 12:08:26 -0400
Subject: doped epoxy resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Henning
Polysciences sell lead, barium and zinc acrylates
which can be used to make radio-opaque acrylic polymers.
Many other metal-methacrylates / acrylates are known, probably commercially
available, including Li, Na, K, Ca, Y, Zr, Ag, Cu, Fe, Cr, etc Europium
methacrylate is apparently fluorescent.

These may provide a bse or xray signal. I have considered using them, but the
polymerization techniques for barium and lead methacrylates sound too hairy,
probably involving ionising radiation capable of penetrating your metals.

Are your voids big enough to image using fluorescence microscopy?
If so, doping the epoxy with a fluorescent dye may work.
see e.g.

http://cee.ce.uiuc.edu/lange/micro/fluorescent.html

or
Fischer, U., B. Kulli, H. Flühler and P. Marschall (1996). Determining the pore structure of shear zone
granite samples by means imbibition with fluorescent resin and image analysis procedures, Nagra,
Wettingen, Interner Bericht 96-79, 39 Seiten.


Chris

} From: Sørensen Henning Sund {Henning.S-at-danfoss.com}
To: Microscopy-at-sparc5.microscopy.com


Henning,
I assume that you want to be able to distinguish between particles, the
space between particles, and the pores that are in the particles.

I have found, as you, that powders mixed with epoxy do not work.

Struers sells a fluorescent dye that you can add to epoxy. I have also used
a blue dye (Oil blue) that is used in the petroleum industry to color
lubricants and fuels. This is very soluble in epoxy.

Everett Ramer
Cellomics, Inc.
Pittsburgh, PA

-----Original Message-----
} From: Sørensen Henning Sund [mailto:Henning.S-at-danfoss.com]
Sent: Wednesday, September 25, 2002 8:34 AM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

I am currently investigating the properties of a permanent magnetic
material.
I need to distinguish between Nd-Fe-B particles, epoxy binder and porosities
on BSE images obtained on metallographic sections. No problem in singling
out the Nd-Fe-B, but do any of you have a suggestion of how to pick up the
porosities?

What we have tried up till now is to fill out the sectioned pores with a mix
of epoxy and fine silicon powder followed by repolishing to the same level.
No succes - the particles were too coarse.
I vaguely remember that I have heard about "doped epoxy resins" for this
kind of purpose. Perhaps some of you know a recipe or supplier.

Thank you,

Henning Sund Sørensen
Materials- and Process Consultant
Technology Centre
Danfoss A/S
6430 Nordborg, Denmark


From daemon Wed Sep 25 11:21:10 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Wed, 25 Sep 2002 12:12:34 -0400
Subject: FW: doped epoxy resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Henning,
Sorry, I thought you were using optical microscopy.

For SEM work you can add iodoform (CHI3) to epoxy to increase the contrast
between the epoxy you use for mounting and the epoxy you use to fill the
pores.

Everett Ramer



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers,

I am currently investigating the properties of a permanent magnetic
material.
I need to distinguish between Nd-Fe-B particles, epoxy binder and porosities
on BSE images obtained on metallographic sections. No problem in singling
out the Nd-Fe-B, but do any of you have a suggestion of how to pick up the
porosities?

What we have tried up till now is to fill out the sectioned pores with a mix
of epoxy and fine silicon powder followed by repolishing to the same level.
No succes - the particles were too coarse.
I vaguely remember that I have heard about "doped epoxy resins" for this
kind of purpose. Perhaps some of you know a recipe or supplier.

Thank you,

Henning Sund Sørensen
Materials- and Process Consultant
Technology Centre
Danfoss A/S
6430 Nordborg, Denmark


From daemon Wed Sep 25 11:35:56 2002



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Wed, 25 Sep 2002 13:30:29 -0300
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Quite so! Tony has hit the nail on the head here. Over the years
I've seen "real" photographic plates that I was extremely proud of
look really rather awful in publication, and those that I wrote off
as "the best that could be done with what I had" turn out not
so bad looking in the end. The same goes for digital plates. In fact,
some of the better ones turned out to have been scanned from
inkjet prints submitted for review! I think the skill of the guys
in the print shop have a lot to do with the final output.

My 3.3 cents (Canadian)....

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman
--

Rosemary has highlighted a point which has been of great concern to me
as a manager of an EM lab in the dawning (dawned !) digital era. With
the distribution of images electronically, locally or to journals afar,
we no longer have the same level of final, critical, control of the
quality of the image. I guess that there is no ready solution just the
passing of an age - though it is perhaps dubious that we had that much
'control' over the final rendering anyway !!

Tony

Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
Mob. 082 782 9640
website: www.nu.ac.za/microscopy.asp
Email: bruton-at-nu.ac.za
Postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa



From daemon Wed Sep 25 13:24:04 2002



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Wed, 25 Sep 2002 14:15:21 -0400
Subject: RE: Printers, again

Contents Retrieved from Microscopy Listserver Archives
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Our facility employs several different printer technologies to display our
work. Ultimately the final use of the printed material will determine which
print technology is select. For example, routine images / spectra are
printed on HP laser printers (B & W) or deskjet printers (color). When
higher quality is necessary we use a Kodak 8650 with "extra-life" ribbons
(Black-BX or Color-CMYX). For high volume B & W print jobs that require a
higher quality than our laserjet technologies we use a codonics NP1650
(thermal-directvista paper) printer. And finally, we occasionally use a
color laser to output color prints or reports containing color information.
The vast majority of our work is run through the laser and inkject printers,
however, our work is enhanced by having availability of all the
technologies. The single most import upgrade to our laboratory (in regard
to printing) has been the ability to access printers from throughout the R &
D building. We have access to dozens of laser and inkjet printers,
including color laser printers. We are fortunate to have a 1996 vintage
Kodak that has performed flawlessly and a codonics that was purchased 2
years ago which also has performed satisfactorily. Bottom line, if you have
them, use them!



PS Sergey,
it has been my experience that Kodak's extra-life coating assists in
protecting the image from minor water damage, bleeding, and fading commonly
encountered with the 3 color CMY or 4 color CMYK ribbons. This is available
in either black or CMY versions only, to my knowledge there is no CMYK
version with the extra-life option. Expensive.....definitely! But in our
line of work the quality of the work is often judged by the presentation of
the results, as much, or more so than the content of the data. Sad but
true.


} John A. Robson
} _____________________________________________________
}
} Boehringer Ingelheim Pharmaceuticals, Inc.
} Research and Development
} 900 Ridgebury Road / P. O. Box 368
} Ridgefield, CT 06877-0368
}
} phone: 203.798.5640
} fax: 203.798.5698
} email: jrobson-at-rdg.boehringer-ingelheim.com
}
}
}
} -----Original Message-----
} From: Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
} Sent: Tuesday, September 24, 2002 10:48 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Printers, again
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I was thinking about printers another day. We do have sort of central
} facility with super-duper Tektronix dye-sub and
} color-laser. Dye-sub prints tends to fade (controversy to the previous
} posting) and our particular printer is slow. Plus $3+ cost for each
} print... Keeping in mind that most publishers accept now digital
} format...
} For myself I decided to use laser-color (somehow it works better than B&W
} laser) for drafts (quick and very reasonable quality, plus CHEAP) and ink
} jet for near-photo quality prints. Right now I am setting up Epson 890
} photo printer. I am going to use archival quality photo paper (more than
} 20 years image life) and 'gray-ink' Lyson cartridges. "Gray-ink" supposed
}
} to deliver outstanding image quality (if did not kill printer's head) and
} extended image life. My current situation is that Epson printer I've
} received a few days ago is defective and will be replaced soon (I
} hope). I'll report (if anybody interesting) my progress with
} 'gray-inks'. Sergey
}
} At 12:12 PM 9/24/02, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Measure the time it takes to print an image of a Codonics 1600 (if you
} can
} } get it to work) and then measure the time it takes to print an image on
} an
} } Epson 980 or similar (the one's we happen to use) and then look at the
} cost
} } difference and see that I'd go for the Epson anytime. They're
} disposable.
} }
} } Again, MY opinion, no one else's!
} }
} } On 9/24/02 2:23 PM, "Warren E Straszheim" {wesaia-at-iastate.edu} wrote:
} }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } I think I would concur with your guesses. Everyone is probably doing
} it on
} } } their own.
} } }
} } } Current inkjet printers CAN do a quite good job rendering images on
} good
} } } paper. I have heard John Mc Kenzie recommend them at his seminars.
} Granted
} } } that many inkjets are still slow compared to other technologies. John
} even
} } } suggests setting up a bank of relatively cheap inkjets to share the
} jobs
} } } and keep through-put up. We haven't gone that route yet.
} } }
} } } Users may also be settling for mediocre prints from their own printers
} not
} } } knowing what quality is available. We have an aging Lexmark Optra Rt+
} that
} } } does a quite good job of printing B/W images on bright white paper. I
} just
} } } passed some prints from it on to a user who had tried printing images
} on
} } } his own printer. He much preferred our quality once he saw the prints.
} He
} } } just assumed, falsely in this case, that he could get the same quality
} on
} } } his own. Maybe you should post a classic image next to your printer so
} your
} } } users see what you can do.
} } }
} } } But having said that, I probably would consider retiring the printer
} } } depending on the cost of repair. Printer technology has come a long
} way in
} } } recent years and the new stuff generally does better for cheaper.
} } }
} } } Warren
} } }
} } } At 10:03 AM 9/24/02 -0700, you wrote:
} } }
} } } } Hi:
} } } }
} } } } I missed MSA this year (fell off my bike, broke some things) so I
} didn't
} } } } get a chance to catch up on the latest and greatest regarding the
} current
} } } } state of the art for printing digital images etc.
} } } }
} } } } Here's my dilema: We have a fancy dye sub printer, but it just broke
} and
} } } } needs repair. We have had it since 1996 and it got lots of use in the
}
} } first
} } } } 4 or 5 years, but business has fallen off lately, like down to one or
} two
} } } } uses in the last several months. I am trying to figure out what is
} } } } happening, my guess is that everyone is using some cheap new ink jets
} in
} } } } their own lab to do their printing. We are a small central services
} type
} } } } lab, our users visit us to use the instruments, then take their image
}
} } files
} } } } with them. The dye sub used to get a lot of use from all kinds of
} } people on
} } } } campus, but no longer.
} } } }
} } } } So, what's up with printing? Do I get the dye sub fixed, or do I save
} the
} } } } $$ and buy a couple of photo ink jet things to do its job? There has
} } been a
} } } } major transformation in imaging technologies here, our darkroom is
} } } } practically abandoned, the dye sub is mostly idle etc. Has printing
} become
} } } } a distributed resource where everyone does it independently, or have
} some
} } } } just given up on printing altogether and everything gets distributed
} and
} } } } submitted in electronic form?
} } } }
} } } } Just curious.
} } } }
} } } } Jonathan Krupp
} } } } Microscopy & Imaging Lab
} } } } University of California
} } } } Santa Cruz, CA 95064
} } } } (831) 459-2477
} } } } jmkrupp-at-cats.ucsc.edu
} } }
} } } -------------------------------------------
} } } No files should be attached to this message
} } } -------------------------------------------
} } } Warren E. Straszheim, Ph.D.
} } } Materials Analysis and Research Lab
} } } Iowa State University
} } } 23 Town Engineering
} } } Ames IA, 50011-3232
} } }
} } } Ph: 515-294-8187
} } } FAX: 515-294-4563
} } }
} } } E-Mail: wesaia-at-iastate.edu
} } } Web: www.marl.iastate.edu
} } }
} } } Scanning electron microscopy, x-ray analysis, and image analysis of
} } materials
} } } Computer applications and networking
} } }
} } }
} } }
} } }
} }
} } --
} } John Mansfield PhD MInstP
} } North Campus Electron Microbeam Analysis Laboratory
} } 417 SRB, University of Michigan
} } 2455 Hayward, Ann Arbor MI 48109-2143
} } Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} } (Leaving a phone message at 936-3352 is preferable to 834-3913)
} } Email: jfmjfm-at-engin.umich.edu
} } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} } Location: Lat. 42! 16' 48" Long. 83! 43' 48"
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}



From daemon Wed Sep 25 13:27:29 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 25 Sep 2002 15:17:13 -0400
Subject: Re: Printers, again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






To: "Jan Rutkowski" {zerutkow-at-cyf-kr.edu.pl}
cc:


years ago, my boss would submit papers to a select handful of
journals...those that consistently did an good job of rendering
electron micrographs. In those days there were still journals that
published pictures in what could be equated with newspaper style,
lots of dots. The larger the dots, the poorer the picture. On
second thought, maybe things haven't changes all that much!
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Sep 25 14:33:49 2002



From: Johnny L. Carson :      jcarson-at-med.unc.edu
Date: Wed, 25 Sep 2002 15:24:05 -0400
Subject: Freeze-fracture double replica booklet

Contents Retrieved from Microscopy Listserver Archives
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We are looking to acquire some additional double replica booklets for
use on a Balzers 400T freeze-fracture plant. Would welcome information
from vendors or individuals with booklets to dispose of.
Johnny Carson
Cell Biology/Ultrastructure Facility
Center for Environmental Medicine, Asthma, and Lung Biology
The University of North Carolina at Chapel Hill
e-mail: jcarson-at-med.unc.edu



From daemon Wed Sep 25 14:52:19 2002



From: i.burba-at-att.net
Date: Wed, 25 Sep 2002 19:45:04 +0000
Subject: Test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




--This is just a test...





From daemon Wed Sep 25 15:44:41 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 25 Sep 2002 16:36:28 -0400
Subject: printers again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I like to make presentations with overheads (transparencies, or films). I have had problems with the quality of inkjet printers printing to transparencies. I have tried Epson printers, and they have given the worst results. And, I have tried more than one model. HP inkjet printers are pretty good. A good sublimation dye printer has given me the best results.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."



-----Original Message-----
} From: Tobias Baskin [mailto:BaskinT-at-missouri.edu]
Sent: Wednesday, September 25, 2002 10:02 AM
To: Microscopy-at-sparc5.microscopy.com


List,
From the single user's perspective, I'd like to put in a plug
for inkjets. I have an Epson 970. I am AMAZED at how well this thing
prints. I keep three grades of paper on hand, two are fancier types
of basic zerox paper, and the third is photopaper. The printer has
controls to adjust the quality and match to the paper. The fancier
white papers are not expensive (at least in USA) and the output on
them is wonderful and perfect for reviewer figures, grant proposals
(14 copies!), and handles color or black and white with no trouble.
And when I put in photopaper the output is phenomenal. I have never
even for a microsec considered using the fancy printers in our core
facility. For my own needs, the cartridges last long enough.

No financial ties to any printer co (or to anything else for
that matter, 8-).

Tobias Baskin
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Wed Sep 25 15:52:13 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 25 Sep 2002 13:46:04 -0700
Subject: Re: doped epoxy resin

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Sorensen,
I have used epoxy doped with Br to track the movement of epoxy while curing
composites and bromine staining of lignin to track the pulping of wood. This
tracking was done by WDS or EDS, not BSE. In brominating the lignin, the
material was just contacted with a bromine solution. The brominated resin
was available commercially. It might be easier, in your case, to adjust the
brightness and contrast of the BSE detector to show a dark gray for the
epoxy binder and black for the porosity. By SE, the pores will show bright
rims and the epoxy should not.
At 02:33 PM 09/25/2002 +0200, you wrote:
}
} Dear Listers,
}
} I am currently investigating the properties of a permanent magnetic
} material.
} I need to distinguish between Nd-Fe-B particles, epoxy binder and porosities
} on BSE images obtained on metallographic sections. No problem in singling
} out the Nd-Fe-B, but do any of you have a suggestion of how to pick up the
} porosities?
}
} What we have tried up till now is to fill out the sectioned pores with a mix
} of epoxy and fine silicon powder followed by repolishing to the same level.
} No succes - the particles were too coarse.
} I vaguely remember that I have heard about "doped epoxy resins" for this
} kind of purpose. Perhaps some of you know a recipe or supplier.
}
} Thank you,
}
} Henning Sund Sørensen
} Materials- and Process Consultant
} Technology Centre
} Danfoss A/S
} 6430 Nordborg, Denmark
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Sep 25 17:35:35 2002



From: Bryan Tracy :      bryant-at-carmel.amd.com
Date: Wed, 25 Sep 2002 15:26:47 -0700
Subject: Printers - FTP - Ribbonless Codonics

Contents Retrieved from Microscopy Listserver Archives
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Hi Listservers,


here is my 3cents from California (everything is more expensive here)

i find the combination of FTP and a ribbonless Codonics very handy.
Password 2 in the FTP command scales the image full page.

i can launch print jobs from my house and when the file is processed by
FTP, the printer is finished before i can walk over and get it from my
desk.

dye subs make very good prints for meetings

i think about 50c a page

bryan tracy
AMD Sunnyvale




From daemon Wed Sep 25 17:35:36 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 25 Sep 2002 17:27:17 -0500
Subject: Staining HIPS-preliminary results

Contents Retrieved from Microscopy Listserver Archives
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Listers,
Thank you for all the help in staining the HIPS with rubber particles.
We have been experimenting and found that, with these samples, immersion in
2% OsO4 gave some contrast to the particles. However, the resulting image
was not very crisp due to the particles having little edge contrast.

Then we took these same immersion stained samples, sectioned, and vapor
stained the sections. This resulted in additional contrast, primarily
helping to outline the edges of the rubber phase, which resulted in very
nice images.

Vapor staining without prior immersion also worked quite well although the
particle staining was not quite as dense. However, we did note more
"pulling" of particles throughout the sample. Apparently the immersion in
osmium just firmed up the sample enough to section with less distortion just
as some of you predicted.

Thanks again for all the advice.
Debby



Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Wed Sep 25 19:08:51 2002



From: rita1027w40-at-msn.com
Date: Tue, 24 Sep 2002 15:46:40 +1000
Subject: CC... IS THIS YOURS?..................... 8646epZm5-382Uaph4331Hk-22

Contents Retrieved from Microscopy Listserver Archives
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I agree with your sentiment, Ritchie, but you are wrong about what
"vulgarity" is. What you have expressed is mere symbolism with no
substance. If Allen had been vulgar in any way, we would have heard from
the net gods immediately. Since that has not occurred, I must disagree
emphatically with the notion that any vulgarity has been used at all.
Further, I was dead on with Allen with everything he said until he suggested
firing a donkey. I saw no relevance in that part of his argument at all,
but then there is nothing in my past that would permit me to claim any
programming power, so I may have read that variable name out of context.
Finally, my son IS a programmer, and I take issue with the notion that such
wonderful folk are ever vulgar, even though they all seem to take great
delight in using the phrase "data is" which is, after all, syntactically
incorrect.

So, TIFF-UNIX or TIF-WIN to you all, and to all a good night.

Of all the anatomists I know I am among the luckiest. I watched a Technai
being assembled all day and will watch a Quanta arrive tomorrow, and I am
the only one in line for one, and the operator for both. Since the last SEM
I ran was over ten and the TEM over 25, I am a lucky S.O.B.

When Mama spoke about the relationship of cleanliness to Godliness, she was
referring to soap, as I recall!

And Allen, I appreciated the lesson in TIFFology despite the zoological
miscue.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Geology-Astronomy
West Chester University of Pennsylvania
Schmucker Science Center II
South Church street & Rosedale Avenue
West Chester, PA, 19383, USA
Phone: 610-738-0437
FAX: 610-738-0437
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/
http://www.wcupa.edu/_visitors/


-----Original Message-----
} From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz]
Sent: Tuesday, September 24, 2002 11:07 PM
To: "David_Bell-at-millipore.com"-at-sparc5.microscopy.com
Cc: microscopy-at-microscopy.com



Oh, come on!

That's not vulgarity!

Vulgarity is %#&(*$-at-&&$!!!!, and &*(^&*$%(&*($.

And how would vulgarity weaken the force of his argument, anyway?

Let's not be toooooo precious.

cheers

rtch





} From: "David_Bell-at-millipore.com"-at-sparc5.microscopy.com
To: "ars-at-sem.com" {ars-at-sem.com}
Copies to: jfmjfm-at-umich.edu, microscopy-at-microscopy.com


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===========================================
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Before you say ''Bull'', please read the following. This is the letter you have been
hearing about on the news lately. Due to the popularity of this letter on the Internet,
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Their findings proved once and for all that there are ''absolutely NO Laws prohibiting
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============================================

Here is another testimonial: "This program has been around for a long time but I never
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program is to follow the simple steps and NOT change anything.''
More testimonials later but first,

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=====Order all 5 reports shown on the list below =====

For each report, send $5 CASH, THE NAME & NUMBER OF THE REPORT YOU
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YOUR ENVELOPE TOP LEFT CORNER in case of any mail problems.

=== When you place your order, make sure you order each of the 5 reports. You will
need all 5 reports so that you can save them on your computer and resell them.
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Within a few days you will receive, vie e-mail, each of the 5 reports from these 5 different
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IMPORTANT - DO NOT alter the names of the people who are listed next to each report,
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2.... Move the name & address in REPORT # 4 down TO REPORT # 5.
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5.... Move the name & address in REPORT # 1 down TO REPORT # 2
6.... Insert YOUR name & address in the REPORT # 1 Position. PLEASE MAKE SURE
you copy every name & address ACCURATELY!

============================================

**** Take this entire letter, with the modified list of names, and save it on your computer.
DO NOT MAKE ANY OTHER CHANGES.

Save this on a disk as well just in case if you loose any data. To assist you with marketing
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============================================
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3..... $5,000 +
4..... $50,000 +
5..... $500,000 ........

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============================================
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============================================
Advertising on the net is very very inexpensive and there are hundreds of FREE places to
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=========== AVAILABLE REPORTS ============
ORDER EACH REPORT BY ITS NUMBER & NAME ONLY. Notes: Always send
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============================================
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__________________________________________________
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_________________________________________________
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__________________________________________________
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_________________________________________________
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_________________________________________________
$$$$$$$$$ YOUR SUCCESS GUIDELINES $$$$$$$$$$$

Follow these guidelines to guarantee your success:

=== If you do not receive at least 10 orders for Report #1 within 2 weeks, continue
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=== After you have received 10 orders, 2 to 3 weeks after that you should receive
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sending e-mails until you do.

=== Once you have received 100 or more orders for Report #2, YOU CAN RELAX,
because the system is already working for you, and the cash will continue to roll in !
THIS IS IMPORTANT TO REMEMBER: Every time your name is moved down on the
list, you are placed in front of a Different report. You can KEEP TRACK of your
PROGRESS by watching which report people are ordering from you. IF YOU WANT
TO GENERATE MORE INCOME SEND ANOTHER BATCH OF E-MAILS AND
START THE WHOLE PROCESS AGAIN. There is NO LIMIT to the income you can
generate from this business !!!

============================================
FOLLOWING IS A NOTE FROM THE ORIGINATOR OF THIS PROGRAM: You have
just received information that can give you financial freedom for the rest of your life, with
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few weeks and months than you have ever imagined. Follow the program EXACTLY AS
INSTRUCTED. Do Not change it in any way. It works exceedingly well as it is now.

Remember to e-mail a copy of this exciting report after you have put your name and
address in Report #1 and moved others to #2 ...........# 5 as instructed above. One of the
|people you send this to may send out 100,000 or more e-mails and your name will be
on every one of them.

Remember though, the more you send out the more potential customer you will reach.
So my friend, I have given you the ideas, information, materials and opportunity to become
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======== MORE TESTIMONIALS ============
"My name is Mitchell. My wife, Jody and I live in Chicago. I am an accountant with a major
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==========================================
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===========================================
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============================================
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Life is beautiful, Thanks to the internet.". Fred Dellaca, Westport, New Zealand
============================================
ORDER YOUR REPORTS TODAY AND GET STARTED
ON YOUR ROAD TO FINANCIAL FREEDOM !
===========================================








660l3


From daemon Thu Sep 26 07:13:13 2002



From: =?iso-8859-1?Q?An=F0elka?= Tonejc :      andelka-at-phy.hr
Date: Thu, 26 Sep 2002 14:03:05 +0200
Subject: Congress Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Announcement for 6th Multinational Congress on Microscopy
(organised by Croatian, Austrian, Czechslovak, Hungarian, Italian and Slovenian
Society for Electron Microscopy)
June 1-5, 2003
Pula, Croatia

The Congress is open for the contributions dealing with all aspecs of
microscopy topics in materials science, biomedical research and
instrumentation.

Please visit the web site

http://www.6mcm.kbsm.hr/

Prof. Andjelka Tonejc
Department of Physics
Zagreb, Croatia




From daemon Thu Sep 26 10:39:56 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Thu, 26 Sep 2002 10:30:12 -0500
Subject: paper references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all interested in paper:
I should have included some references that have been useful to me in
my studies of papers.
1.) "New electron and light optical techniques for examining paper making ,
Donald L. Gibbon, George C. Simon, and Richard C. Cornelius, from TAPPI
Journal 10/89. ( discusses the embedding and metallurgical polishing
procedures, I don't do the infiltration steps since the vacuum impregnation
works better for me )

2.) "Determining paper-coating thickness with electron microscopy and image
analysis" , Richard A. Peterson and Christopher L. Williams. from TAPPI
Journal 10/92 , ( Discusses using the ultramicrotome for cross-sections)

3.) "The Application of Microtomy in the Paper Industry" , Eckehard
Saverin, Claudia Bachie-Stolz, from Jung Application Brief, Leica. ( this
has some nice light micrographs of stained paper cross-sections and tips
for embedding )
Terry Ellis
Hallmark Cards Inc.
Kansas City, MO , USA
816-545-6573




From daemon Thu Sep 26 10:39:56 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 26 Sep 2002 08:27:53 -0700 (PDT)
Subject: Sensys/PCI/MacG4 problem

Contents Retrieved from Microscopy Listserver Archives
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Hello Microscopists,

we have a Photometrics Sensys CCD camera mounted on our microscope being
run via a Power Mac 9600 with a PCI Snapper card. This works good.
However, when we have tried to upgrade to a Power Mac G4 (with a new model
II PCI card), our system no longer recognizes the camera being there.
We supposedly have the correct drivers installed. Has anyone encountered
this problem and found a fix?

Robert Underwood
U of Washington
Dept of Med, Div of Derm
Seattle WA



From daemon Thu Sep 26 15:42:31 2002



From: David Rothbard :      rothbardD-at-netscape.net
Date: Thu, 26 Sep 2002 16:30:02 -0400
Subject: paper references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With apologies for tooting my own horn, you might look at....

Rothbard, D.R. (2002) Applied Microscopy for the Paper Industry.
Microscopy & Microanalysis 2002, Proceedings, Cambridge University
Press, New York, p. 178-179

Rothbard, D.R. (In Press) Electron Microscopy for the Pulp and Paper
Industry. In Z. Li (Ed.), Industrial Applications of Electron
Microscopy, Marcel Dekker, New York

Also:

OW Gregersen, PO Johnsen, T Helle. Small-scale topographic variations of
newsprint surfaces and their effects on printing ink transfer
distribution. J. Pulp Paper Sci. 21(10): J331-J336, 1995

A Donald, L Jenkins. Use of environmental scanning electron microscope
for observation of the swelling behavior of cellulosic fibers. Scanning
19(2): 92-97, 1997

GJ Williams, JG Drummond. Preparation of large sections for the
microscopical study of paper structure. J. Pulp Paper Sci. 26(5):
188-193, 2000

RA Parham. Electron Microscopy of Pulp and Paper. Wood Science. 6(3):
245-255, 1974


David Rothbard
Earthborn Solutions
rothbardD-at-netscape.net



From daemon Thu Sep 26 18:02:15 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.Princeton.EDU
Date: Thu, 26 Sep 2002 18:49:57 -0400
Subject: TMC Vibration Isolation Platform

Contents Retrieved from Microscopy Listserver Archives
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We have a TMC MICRO-g Anti-Vibration Isolation Platform Model #
65-17171-01 that we used to support a JEOL 100C TEM that we wish to sell.
The platform is 86" wide by 71" deep with a seating cut out that is -at- 30"
wide narrowing down to 12" and is about 38" deep. System has 4 isolation
gimble feet to float the platform. With the feet, it requires an area that
is 97" x 82". The weight of unit is about 2200 lbs. Asking $3,000.00 or
best offer plus shipping and handling charges. Interested parties should
contact


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432 or

Bill Huston
bhuston-at-molbio.princeton.edu
609-258-6205




From daemon Thu Sep 26 18:58:45 2002



From: DrJohnRuss-at-aol.com
Date: Thu, 26 Sep 2002 19:50:42 EDT
Subject: Image Analysis Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The three-day intensive hands-on workshop on Image Processing and Measurement
presented by John Russ (author of "The Image Processing Handbook" and
"Practical Stereology") through the North Carolina State University
Department of Continuing and Professional Education is now in its 21st year.
The upcoming course dates are November 6-8, 2002, and May 21-23, 2003, in
Raleigh, and June 9-11, 2003, at the Danish Technological Institute in
Taastrup, Denmark (near Copenhagen). This course has generated highly
favorable reviews from the thousands of previous students. The primary focus
is on images from various types of microscopy, with practical guidance in
correcting imaging defects, enhancing the images for presentation and
measurement, and performing stereological meaningful measurements on them.
Textbooks and computer software are provided to attendees. Lab sessions with
an opportunity to bring your own images makes this course immediately useful
and highly productive.

There are still a few openings available for the November session. For full
information on the course, including outlines, faculty information, a
downloadable brochure, and on-line registration, go to

{http://members.aol.com/ipcourse}

Class size is limited to maintain a high ratio of instructors to students, so
make your reservation now. You may also contact Cindy Allen at NCSU
Continuing Education, at 919-515-8171


From daemon Thu Sep 26 23:00:16 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 26 Sep 2002 20:54:08 -0700
Subject: Damascene structure analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Are there any folks out there that are working with
analysis of failure mechanisms or failure potential
areas of damascene ICs?

I would appreciate conversing with anyone doing
this using FIB and/or SEM. Methods of analyzing
damascene structures is at issue. A particular
goal is to determine how commercial damascene
devices survive in a hostile environment. I know that
there are incipient failure mechanisms in small
feature size ICs. The issue is to discover and
quantify these sufficiently to make them validated.

Off-line responses are invited.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA



From daemon Fri Sep 27 00:10:14 2002



From: Arthur Day :      ard-at-ansto.gov.au
Date: Fri, 27 Sep 2002 15:00:11 +1000
Subject: Re: paper references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Trouble is Terry they're all just "paper studies" :-)


}
} To all interested in paper:
} I should have included some references that have been useful to me in
} my studies of papers.
} 1.) "New electron and light optical techniques for examining paper making ,
} Donald L. Gibbon, George C. Simon, and Richard C. Cornelius, from TAPPI
} Journal 10/89. ( discusses the embedding and metallurgical polishing
} procedures, I don't do the infiltration steps since the vacuum impregnation
} works better for me )
}
} 2.) "Determining paper-coating thickness with electron microscopy and image
} analysis" , Richard A. Peterson and Christopher L. Williams. from TAPPI
} Journal 10/92 , ( Discusses using the ultramicrotome for cross-sections)
}
} 3.) "The Application of Microtomy in the Paper Industry" , Eckehard
} Saverin, Claudia Bachie-Stolz, from Jung Application Brief, Leica. ( this
} has some nice light micrographs of stained paper cross-sections and tips
} for embedding )
} Terry Ellis
} Hallmark Cards Inc.
} Kansas City, MO , USA
} 816-545-6573



From daemon Fri Sep 27 07:15:18 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Fri, 27 Sep 2002 08:01:36 -0400
Subject: SEM installation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




To any service-type folk looking to travel:


Earl Weltmer has asked me to post the following:

"Please ask for contact to install a Hitachi S-570 SEM in Hang Zhou,
China. near Shanghai."

If you're capable and interested, please respond directly to Earl.

Earl Weltmer [mailto:earlw-at-sbcglobal.net]

Thanks,

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA




From daemon Fri Sep 27 07:47:14 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Fri, 27 Sep 2002 08:40:10 -0400
Subject: Re: Jeol 733 X-Ray Maps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Roy,
The expanations you've gotten concerning the Rowland Circle are
correct, hence, if you have an EDS that will detect and map what you're
looking for, use it.

40x is getting pretty low in mag, but the following technique has been
used to do low mag WDS mapping. Use your electronic raster rotation to
make the band of dots horizontal. (If you don't have the raster
rotation module, you're out of luck.) Set your scan generator for a
single line and move that line to the top of your CRT. Peak the
countrate on your spectrometer by changing the spectrometer
crystal/detector position and note the position where it peaks. Move
the line to the bottom of your CRT and repeak your counts. Note the
spectrometer position.

Now you need to do a little calculating. Determine how long your record
sweep takes and find a speed for your spectrometer that will take you
between the 2 positions determined above. Set the spectrometer for the
first position and start it scanning towards the second position at the
same time you start the record scan. This won't give a perfect map at
very low mags, but it is much better than that little diagonal band.

What you're doing here is playing with the effective position of the
Rowland Circle. At low enough mags the system becomes so detuned that
you will get a major signal roll-off at both the top and botton of the
sweep, but if you need to isolate a particular line or a light element
and do it at low mag, or you just don't have an EDS system, this is
about the best you can do.

Good luck,

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Beavers, Roy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Fri Sep 27 09:09:31 2002



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Fri, 27 Sep 2002 09:56:31 -0400
Subject: quantitative composition analysis by EDS in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I have some questions concerning quantitative composition analysis by EDS
in a TEM.
1. how do I do the quantitative analysis correctly? Is it a truly
standardless method? What precautions should be taken?

2. I checked a EuS sample. I used Eu L lines and S K line. It gives about
70%Eu and 30%S, which is way off the stoicheometric value. The sample is
believed to be not far from 50%Eu and 50%S.
The result of a GaAs sample seems reasonable.
So what is the reason for this result? because of using L lines?

I would appreciate very much help and suggestions on this.

Regards
Yan Xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Fri Sep 27 09:40:58 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Fri, 27 Sep 2002 09:33:45 -0500
Subject: Schematic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,


Looking for a schematic for a CARY 401 Vibrating Reed Electrometer.
If anyone can help please contact me directly.
Thanks
Roy Beavers
Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, Tx. 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-mail.smu.edu




From daemon Fri Sep 27 10:24:05 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Fri, 27 Sep 2002 08:11:10 -0700 (PDT)
Subject: SEM: Pt vs Mo Apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I notice in some catalogs, that one has the option of
ordering either Platinum or Molybdenum apertures.
what are the advantages and disadvantages between the
two?

Stu Smalinskas
Senior Metallurgist
SKF NATC
Plymouth, Michigan

__________________________________________________
Do you Yahoo!?
New DSL Internet Access from SBC & Yahoo!
http://sbc.yahoo.com


From daemon Fri Sep 27 10:40:11 2002



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Fri, 27 Sep 2002 11:27:51 -0400
Subject: Re: quantitative composition analysis by EDS in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:01 AM 9/27/02 -0500, you wrote:
} You didn't say if you were reporting your results as mass fraction or
} atomic fraction. The two values will be similar for GaAs since they have
} atomic masses of 70 and 75. But Eu has a mass of 152 while S has a mass of
} 32, so the results should be about 83:17 by mass. BTW, how do you know the
} EuS sample is really a 1:1 stoichiometry?

Warren,

Thanks for the suggestions. They are atomic fractions.

I used the target materials. It was made stoichiometric.


Regards
Yan Xin



From daemon Fri Sep 27 11:53:54 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Fri, 27 Sep 2002 12:42:40 -0400
Subject: Color Laser Printer

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Has anyone tried HP LaserJet 4600 Color Laser Printer for SEM pictures? Its
supposed to print 17ppm BW and color with 600dpi with HP Imageret 2400 dpi.
Any suggestions, concerns, or recommendations before we decide to purchase
one?

Really appreciate your help for so many times!!!

Thanks
Pavel



From daemon Fri Sep 27 14:20:06 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 27 Sep 2002 15:09:58 -0400
Subject: Re: quantitative composition analysis by EDS in TEM

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Yan-

You questions (especially number 1) can't be answered properly in an e-mail
like this. Very brief and incomplete answers are:

There are lots of things that must be done before you can trust an EDX
analysis in the TEM. You need to understand the electron probe
characteristics (assuming you are needing high spatial resolution
analysis), and any spurious responses from your microscope/X-ray analyzer
configuration, as well as any peculiarities in your particular x-ray
detector. I don't think these issues are relevant to your particular
analysis, though.

The age and manufacturer of your system will determine how trustworthy your
"standardless" analyses are - some newer systems give very good results (in
some analyses, at least), while others, using older
techniques/constants/whatever can be quite questionable. A general "rule
of thumb" is that comparing two elements close to each other in the
periodic table (such as Ga and As) is more reliable than comparing elements
far removed from each other, especially if you are using x-ray lines of
different families (as in your case). The only way to be sure is to
analyze a sample of known composition (in effect, determine your own standards)

Sample thickness is very important. In your case, the S-K line is at about
2.3 KeV, while the Eu-M absorption edge is at about 1.16KeV. The Eu-L line
you are measuring is at 5.85Kev, and the S-K absorption edge is at
2.5KeV. Calculating (from Kurt Heinrich's tables) the mass absorption
coefficients for S and Eu in the sample gives 2149 cm^2/gm for S and 244
for Eu. If you are working in a 200KV or 300KV TEM, it would be easy to be
in an area thick enough that an absorption correction would be
essential. If your sample is bent so that the area you are analyzing is
tilted away from the x-ray detector, it will make this problem worse. This
effect would tend to give a low S analysis.

Sometimes the microscope geometry can be incorrect (if the sample height is
wildly wrong, for example), and this, too, can reduce the intensity of the
low-energy lines more than the high energy ones.

These problems of absorption, whether due to thickness or geometry, are
easily seen in the shape of the bremsstrahlung background. If there is
anyone in your lab who has lots of experience with your particular system,
they should be able to look at the spectra and tell at once if the shape of
the bremsstrahlung is near-enough correct.

Of course your software must be set up with the correct operating voltage,
but that would make only a relatively small change in the result, (as
opposed to the situation in the SEM, where the correct voltage is critical).

I don't know about europium compounds, but iron sulphides are not stable
under the electron beam. They lose S as time passes. Taking a number of
analyses for short times at the same point can demonstrate this (the S
content will progressively fall).

I hope I don't depress you too much!!!

Tony.



At 09:56 AM 9/27/2002 -0400, Yan Xin wrote:
} I have some questions concerning quantitative composition analysis by EDS
} in a TEM.
} 1. how do I do the quantitative analysis correctly? Is it a truly
} standardless method? What precautions should be taken?
}
} 2. I checked a EuS sample. I used Eu L lines and S K line. It gives
} about 70%Eu and 30%S, which is way off the stoicheometric value. The
} sample is believed to be not far from 50%Eu and 50%S.
} The result of a GaAs sample seems reasonable.
} So what is the reason for this result? because of using L lines?
}
} I would appreciate very much help and suggestions on this.
}
} Regards
} Yan Xin


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Fri Sep 27 16:43:29 2002



From: Edy Junop Widjaja :      ejw923-at-casbah.it.northwestern.edu
Date: Fri, 27 Sep 2002 18:19:17 -0500 (CDT)
Subject: Re: quantitative composition analysis by EDS in TEM

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As you probably know, Ladd is the world's largest producer of burr-free
apertures for both EM's and a wide variety of other applications. The
question of moly vs. platinum has come up quite often over the past 50
years. It all boils down to customer preference.

Some points:
1. The cost for both is the same.
2. Some users feel moly being harder will last longer.
3. Platinum can be flamed, moly requires an evaporator.

Since a single hole aperture is quite inexpensive and lasts a long time in a
modern EM they tend to become disposable after one or two cleanings. So we
suggest platinum. Just pull it out, flame it and put it back in.

Multi-hole strips are more expensive and evaporator cleaning will lengthen
the life so moly might be the best choice. Some strips and plates we do
have 20 or more holes.

Based on our experience we have the following general recommendations:
single hole EM disc - platinum
multi-hole plate or strip - moly
X-ray plates or discs - tantalum
satellites - stainless steel
ION beam - moly
infrared - stainless steel

Disclaimer: Ladd sells EM supplies and accessories

John Arnott
Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Kestutis Smalinskas" {smalinskas-at-yahoo.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, September 27, 2002 11:11 AM


What do you mean by "it gives about 70% ...."?
Is it something the computer spits out when you click composition
analysis? Hope it is not.

A 'correct' quantitative composition analysis requires a reasonable solid
background on what EDS is about. A book by goldstein (SEM and X-ray
microanalysis) covers reasonable background on this topic.

There are artifacts and complications. To mention some: silicon x-ray
escape peaks, silicon fluorescense, sum peaks... these are probably not
taken into consideration by the computer in 'spitting' the final
composition. Tilt angle (detector-sample), homogeneity of samples,
absorption, are some of other things you should take into consideration
when doing a 'more precise' quantification.

Cliff-Lorimer constants can give some idea about relative composition for
different elements. However, to do a reasonable 'correct' quantification,
I will definitely suggest a standard with known composition.

DTSA is a program that allow data manipulation (such as background
substraction), and simulating different parameters (such as angle, etc).
It's free, but works only on Mac.

Alternatives are using other method to do composition measurement. There
are other methods that give a (much) better accuracy than EDS in TEM,
unless you are looking at a small feature in your sample.

Edy Widjaja
Materials Science and Engineering
Northwestern University
reply to : e-widjaja-at-northwestern.edu
office : 847-491-7809 lab : 847-491-3281
http://www.numis.nwu.edu/internet/Staff/edy


On Fri, 27 Sep 2002, Yan Xin wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear listers,
}
} I have some questions concerning quantitative composition analysis by EDS
} in a TEM.
} 1. how do I do the quantitative analysis correctly? Is it a truly
} standardless method? What precautions should be taken?
}
} 2. I checked a EuS sample. I used Eu L lines and S K line. It gives about
} 70%Eu and 30%S, which is way off the stoicheometric value. The sample is
} believed to be not far from 50%Eu and 50%S.
} The result of a GaAs sample seems reasonable.
} So what is the reason for this result? because of using L lines?
}
} I would appreciate very much help and suggestions on this.
}
} Regards
} Yan Xin
} =======================================
} Yan Xin (Ph.D)
} Magnet Science & Technology
} National High Magnetic Field Laboratory
} Florida State University
} 1800 E. Paul Dirac Drive
} Tallahassee, FL 32310
} Tel: (850) 644 1529
} Fax: (850) 644 0867
} ========================================
}
}
}
}
}




From daemon Fri Sep 27 22:52:30 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 27 Sep 2002 23:38:57 -0500
Subject: Pt vs. Moly apertures

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Stu Smalinskas wrote:
====================================================
I notice in some catalogs, that one has the option of ordering either
Platinum or Molybdenum apertures. what are the advantages and disadvantages
between the two?
====================================================
This is one of our most frequently asked questions and we have tried to
cover it on our URL
http://www.2spi.com/catalog/apt/aptintro.html

Disclaimer: SPI Supplies has offered both platinum and moly apertures for
some number of years to our customers in the electron microscopy world. Our
vested interest is making sure our customers gets what is best from their
applications standpoint.

Chuck

PS: Remember that we are striving to be 100% paperless, therefore there
are no paper copies kept of this correspondence. Please be sure to always
reply by way of "reply" on your software so that the entire string of
correspondence can be kept in one place.
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sat Sep 28 02:47:00 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Sat, 28 Sep 2002 00:28:55 -0400
Subject: Re: quantitative composition analysis by EDS in TEM

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on 9/27/02 9:56 AM, Yan Xin at xin-at-magnet.fsu.edu wrote:

}
} I have some questions concerning quantitative composition analysis by EDS
} in a TEM.
} 1. how do I do the quantitative analysis correctly? Is it a truly
} standardless method? What precautions should be taken?
}
} 2. I checked a EuS sample. I used Eu L lines and S K line. It gives about
} 70%Eu and 30%S, which is way off the stoicheometric value. The sample is
} believed to be not far from 50%Eu and 50%S.
} The result of a GaAs sample seems reasonable.
} So what is the reason for this result? because of using L lines?
}
} I would appreciate very much help and suggestions on this.
}
Dear Yan Xin,
1) There are standardless methods, which depend on theoretical
calculations. When last I looked, these calculations were somewhat
uncertain, although they may have improved. If you have standards for Eu
and S in a matrix that is as close as possible to that of the sample you are
examining, you can check whether the standardless analysis matches your
standards.
2) I suspect that the S may be volatile, but I may be way off base. If
you are using the correct parameters for production of the Eu L line, the
calculation should be correct, so use of the L line should not be the reason
for the result. If, however, your standardless analysis program
automatically inserts the parameters for K lines without giving you a
choice, this will obviously lead to an error.
Yours,
Bill Tivol



From daemon Sat Sep 28 03:05:38 2002



From: GAO Yihua :      GAO.Yihua-at-nims.go.jp
Date: Sat, 28 Sep 2002 16:57:41 -0700
Subject: Position hunting

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Dear colleagues,

I am a young researcher on materials and transimission electron
microscopy for 10 years(5 patents, 15 papers including 1 paper in one of the
best journals and some in famous SCI journals). I am hunting a reseach
position (or post-doctoral postion). If someone has such a position and is
interested in me, keep in touch with me.

Thank you very much

Yours sincerely

Gao Yihua
----------------------------------------------------------------------------
Dr. Yihua Gao (Y.H. Gao)
Advanced Materials Laboratory and Nanomaterials Laboratory,
National Institute for Materials Science,
Namiki 1-1, Tsukuba, Ibaraki 305-0044, Japan
Email: GAO.Yihua-at-nims.go.jp;
gaoyihua-at-yahoo.com
TEL: 81-298-513354(ext.662)
Fax: 81-298-516280
----------------------------------------------------------------------------



From daemon Sat Sep 28 17:32:07 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Sat, 28 Sep 2002 15:14:37 -0700 (PDT)
Subject: Re: Pt vs Mo Apertures

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Thanks for your answers. I probably should have
mentioned that I clean my apertures mechanically. If
you remember, I'm the one that posted about using 1 to
6 micron diamond and metallographic cloth to polish
apertures, so baking under vacuum (or without vacuum)
is not an issue.

Stu Smalinskas
Metallurgist
SKF NATC
Plymouth, Michigan

__________________________________________________
Do you Yahoo!?
New DSL Internet Access from SBC & Yahoo!
http://sbc.yahoo.com


From daemon Sat Sep 28 21:55:51 2002



From: Allen Sampson :      ars-at-sem.com
Date: Sat, 28 Sep 2002 21:43:17 -0700
Subject: RE: Pt vs Mo Apertures

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A little more info, as usual, helps.

Best bet is dump the diamond paste and use regular polishing compound on
moly apertures. Moly's hardness along with the softer polish will assure a
long life for the apertures. Regular cotton twill lint-free cloths or
Kimwipes work well for polishing.

Here's another clue, one I haven't shared with folks here yet. I spent
years looking for a simple compound for cleaning optics parts that I could
take to customer labs and use without worry about their many different
environmental concerns. I found it a couple of years ago and have been
using it with good success. As far as I am aware, there are no
restrictions or serious cautions on its use or disposal.

The compound is Sodium Metasilicate, a popular replacement for TSP based
wall cleaners that's available in just about every hardware store. I've
used a mild (a couple of grams to 500mL water, nothing exact) solution to
ultrasound the parts in. It takes a while, 20 - 30 minutes, but gets rid
of most of the contamination on its own. What remains is a lot easier to
remove with light polishing. Polished brass parts come out bright. The
package will caution about a mild etching action on polished aluminum and
glass - I've haven't noticed it on either, but it is probably because of
the dilute concentrations I use. Other materials seem unaffected. Perhaps
higher concentrations would clean better on parts not affected by the
etching.

Another nice feature is using water (DI or distilled) as the solvent for
steps that use large amounts of solvents - tends to make you rinse things
better. Any parts needing polishing I then ultrasound in the solution
again to remove the particulates. All parts get well rinsed with water and
a careful hand rinse with a squirt bottle of methanol, ethanol, isopropyl
or acetone to remove any remaining water prior to reinstallation.

In this case, it will help the apertures last longer. In fact, if you
clean them more frequently, you might find that the solution itself will be
sufficient and you won't need to polish them.

If anyone uses this, let me know how it works for you.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Saturday, September 28, 2002 3:15 PM, Kestutis Smalinskas
[SMTP:smalinskas-at-yahoo.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Thanks for your answers. I probably should have
} mentioned that I clean my apertures mechanically. If
} you remember, I'm the one that posted about using 1 to
} 6 micron diamond and metallographic cloth to polish
} apertures, so baking under vacuum (or without vacuum)
} is not an issue.
}
} Stu Smalinskas
} Metallurgist
} SKF NATC
} Plymouth, Michigan
}
} __________________________________________________
} Do you Yahoo!?
} New DSL Internet Access from SBC & Yahoo!
} http://sbc.yahoo.com
}
}
}



From daemon Sun Sep 29 23:11:07 2002



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From daemon Mon Sep 30 07:55:21 2002



From: John Arnott :      ladres-at-worldnet.att.net
Date: Mon, 30 Sep 2002 08:45:44 -0400
Subject: More thoughts on Moly vs. Pt apertures

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Just some additional thoughts on material choice for apertures:

When you speak of a Pt aperture it is probably Pt/Ir. The iridium allows
for easier machining. We do pure platinum and gold apertures but only when
the application demands it.

Another important consideration is the number and sizes of a multi-hole
strip or plate. We have put up to 30 holes, some as small as 1micron, in a
single part. Since the manufacture of burr-free, concentric apertures
requires that the holes be made individually, more holes and smaller sizes
result in more material losses during production. Platinum costs can add
up.

For ultra-small holes and multi-hole parts moly should be used whenever
possible.

John Arnott
Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com




From daemon Mon Sep 30 11:29:36 2002



From: Heeschen, Bill (WA) :      WAHeeschen-at-dow.com
Date: Mon, 30 Sep 2002 12:11:45 -0400
Subject: Position Available - polymer electron microscopist at The Dow Che

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} Please send resumes and direct inquiries to: rccieslinski-at-dow.com
}
} POSITION OPEN
}
} Polymer Electron Microscopist
} The Dow Chemical Company
} Midland, Michigan
}
} Dow’s Corporate R&D Analytical Science Laboratory has a professional level
} opening for a polymer microscopist at it’s Midland Michigan location. The
} primary responsibilities would be to design, conduct and interpret
} electron microscopy experiments to provide key answers to questions
} relating to the development, processing and the performance of various
} materials. Good written and oral communication skills are essential. The
} ability to work both independently and in a team environment is also
} extremely important.
}
} Educational Requirements:
} A Ph.D. or MS degree in a materials or chemical related discipline is
} preferred, but candidates with in a biological science related discipline
} and substantial materials EM work experience would be considered.
}
} Experience Requirements:
} Experience operation of a transmission electron microscope; excellent
} manual dexterity and visual acuity; demonstrated ability to acquire and
} objectively interpret data, and hands-on experience in the preparation of
} specimens for Electron Microscopy, including ultramicrotomy and
} cryo-ultramicrotomy.
}
} Key responsibilities will include:
} * Strong problem solving skills.
} * Active participation in development and project work teams.
} * Interpretation and documentation of work
} * Compliance with safety and quality programs.
}
} Send resumes and direct inquires to: rccieslinski-at-dow.com
}
} Robert C. Cieslinski, Ph.D.
} The Dow Chemical Company
} Corporate R&D, Analytical Sciences
} 1897E Bldg.
} Midland, MI 48667
} Fax: (989) 638-6443
} Email: rccieslinski-at-dow.com
}
} The Dow Chemical Company is the second largest chemical company in the
} world and a leader in science and technology solutions. Come visit Dow
} careers on the web at http://www.dow.com/careers.
}


From daemon Tue Oct 1 09:02:17 2002



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Tue, 1 Oct 2002 15:01:50 +0200 (MET DST)
Subject: SCIA 2003 - Call for Papers (fwd)

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Call for Papers
13th Scandinavian Conference on Image Analysis
SCIA 2003
Göteborg, Sweden, June 29 - July 2, 2003
http://www.hh.se/scia2003

Please notice the new date

The ambition of SCIA is to bring together leading researchers in the
field of image analysis and present an exciting and advanced scientific
program reflecting the state-of-the-art of the discipline. SCIA 2003 is
the thirteenth in a series of conferences arranged every two years. We
look forward to your contributions to this long-standing tradition.

Hosted this year by the Swedish Society of Image Analysis (SSAB), the
conference is sponsored by the International Association for Pattern
Recognition (IAPR) and the Nordic chapters of IAPR.

Scientific Program

The scientific program will include contributed as well as invited
papers. Accepted papers will be published in the conference proceedings
(Springer LNCS). The first day of the conference will consist of special
sessions, such as workshops and/or tutorials.

Contributions covering the topics below are solicited. However, all
submissions addressing issues related to image analysis are strongly
encouraged. The main technical areas are:

- Image feature extraction
- Image understanding
- Grouping and segmentation
- Motion analysis
- Texture analysis
- Color analysis
- Shape analysis
- Computer Vision
- Cognitive vision
- Medical image processing
- Measurement and quantification
- Measurement and visualization
- Image coding and compression
- Multi-modal processing
- Indexing and databases
- Images and the 3-D geometry
- Standards and best practices
- Images and pattern recognition
- Classification
- Applications

Invited Speakers and Workshop/Tutorial Presenters (not yet complete)
Ivar Austvoll, Stavanger University College (NO)
Ewert Bengtsson, Uppsala University (SE)
Lars Bååth, Halmstad University (SE)
Herve Delingette, INRIA, Sophia-Antipolis (FR)
Chris Glasbey, Biomathematics & Statistics Scotland (UK)
Ed Hancock, University of York (UK)
Ioannis Kakadiaris, University of Houston (US)
Rasmus Larsen, Technical University of Denmark (DK)
Jussi Parkkinen, University of Joensuu (FI)
Milan Sonka, University of Iowa (US)

Venue
Situated in the heart of Scandinavia, Göteborg is within easy reach by
air, rail and sea. There are daily flights from all the main European
airports to Göteborg's Landvetter airport. The city center is only 20
minutes away by coach. Founded in 1829, Chalmers University of
Technology is named after the major benefactor, William Chalmers, one of
the directors of the successful Swedish East India Company in Göteborg.
Today, the campus area with its modern conference facilities is situated
in the city center close to hotels, restaurants, theatres, and shopping
centers.

Social Activities
Besides the conference dinner, there will be plenty of opportunities for
social activities including boat cruises in the beautiful Göteborg
archipelago, Liseberg - the largest amusement park in Scandinavia, the
Botanic Garden, and many others.

Instruction to Authors
Max 6 pages according to Springer LNCS format
http://www.springer.de/comp/lncs/authors.html
Look under the heading "Proceedings and Other Multi-author Volumes"
describing format files and providing help. Papers should be submitted
to:
scia2003.papers-at-hh.se

Important Dates
Submission of papers: January 22, 2003
Notification of acceptance: March 22, 2003
Camera-ready papers: May 8, 2003
Conference dates: June 29 - July 2, 2003

Contact Information
http://www.hh.se/scia2003
SCIA2003-at-gbg.congrex.se

Conference Co-Chairs
Josef Bigun
Tomas Gustavsson

Programme Committee
Fritz Albregtsen (NO)
Kalle Åström (SE)
Ivar Austvoll (NO)
Ewert Bengtsson (SE)
Ketil Bo (NO)
Magnus Borga (SE)
Gunilla Borgefors (SE)
Stefan Carlsson (SE)
Henrik Christensen (SE)
Per-Erik Danielsson (SE)
Bjarne Ersböll (DK)
Robert Forchheimer (SE)
Eric Granum (DK)
Anders Heyden (SE)
Jukka Iivarinen (FI)
Peter Johansen (DK)
Fredrik Kahl (SE)
Hans Knutsson (SE)
Björn Kruse (SE)
Rasmus Larsson (DK)
Reiner Lenz (SE)
Tony Lindeberg (SE)
Claus Madsen (DK)
Henning Nielsen (DK)
Ingela Nyström (SE)
Erkki Oja (FI)
Sören Olsen (DK)
Matti Pietikäinen (FI)
Ann Sohlberg(NO)
Örjan Smedby (SE)
Antanas Verikas (SE)
Qin Zhong-Ye (SE)





From daemon Wed Oct 2 01:03:21 2002



From: little-at-earthtech.org (by way of MicroscopyListserver)
Date: Wed, 2 Oct 2002 00:51:28 -0500
Subject: Ask-A-Microscopist: SEM: Cambridge Stereoscan questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (little-at-earthtech.org) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
October 1, 2002 at 21:27:21
---------------------------------------------------------------------------

Email: little-at-earthtech.org
Name: George Luce and Scott Little

Organization: Earthtech International, Inc.

Education: Graduate College

Location: Austin, Texas

Question: We have recently revived a Cambridge Stereoscan 150 SEM
from about 1978. The machine is now operational, but we cannot get
good resolution or focus above a few thousand X magnification.

We would like to communicate with someone familiar with this or a
similar model, who could give us some guidance on improving the
resolution. Details of our efforts can be supplied. We are
technically competent people with backgrounds in physics,
engineering, high voltage, electronics, vacuum, etc.

Thank you.


George Luce (geolucetx-at-aol.com)

Scott Little (little-at-earthtech.org)


Earthtech International, Inc.
Austin, Texas

Phone (512) 342-2185

www.earthtech.org

---------------------------------------------------------------------------


From daemon Wed Oct 2 01:03:21 2002



From: mgrace-at-fit.edu (by way of MicroscopyListserver)
Date: Wed, 2 Oct 2002 00:53:13 -0500
Subject: Ask-A-Microscopist:ESEM Low Vacuum Mode. how useful is it?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mgrace-at-fit.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
October 1, 2002 at 15:16:59
---------------------------------------------------------------------------

Email: mgrace-at-fit.edu
Name: Michael Grace

Organization: Florida Institute of Technology

Education: Graduate College

Location: Melbourne, FL, USA

Question: I am working toward aquisition of a new SEM that will see
use mostly in biological sciences applications, but will also see
some use from materials scientists, chemists, etc. I am considering
one of the new ESEMs (specifically the FEI/Philips Quanta 200.
Question: how useful is the low vacuum mode, esp. for biological
applications? Also interested in feedback from those with other
applications, but I mainly want to be sure that the money is spent
wisely- that we are not getting all the bells and whistles simply
because they exist.

Thank you.

---------------------------------------------------------------------------


From daemon Wed Oct 2 05:18:33 2002



From: Allen Sampson :      ars-at-sem.com
Date: Wed, 2 Oct 2002 05:13:21 -0700
Subject: RE: Ask-A-Microscopist: SEM: Cambridge Stereoscan questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, but are you experienced in electron microscopes? This is definitely a
field that not only encompasses those mentioned, but uses them, as well as
others, all in synergistic manner.

When it comes to resolution, look to your friend, the condenser control (or
spot size or beam current or whatever else it may be called). On that
vintage of Cambridge, I expect it is called spot size. If everything else
is OK, the condenser is the primary control that determines the resolution
of the instrument.

This is because the primary aberration in an electron optic instrument is
chromatic aberration. That is the result of the wide spread in energy
ranges of the electrons emitted from the electron gun. That spread creates
what is known as a spherical aberration that affects the beam in both the X
and Y directions. As they travel through the various electromagnetic
lenses of the SEM, electrons of different energies are affected differently
primarily resulting in a different focal point in the Z axis. The
condenser lens, and associated apertures, are used to reduce this energy
spread.

Here's the deal. The higher the condenser current, the smaller the spot
size and the smaller the beam current. It's hard to explain in text, but
you actually have two condenser lenses. Their purpose is to provide a
de-magnification (reduction in apparent size) of the source of the
electrons. The source is the tungsten filament, or cathode, that emits
electrons from a rather large area of around 100 microns (micrometers).

In the process, there are a couple of imaginary images formed, or crossover
points, where apertures or electron absorbers with small central openings
are located. Electrons of different energies will be affected differently
by the electromagnetic lenses - electrons of lower energy will be displaced
more than those of higher energies. The apertures will capture those
electrons that have an energy lower or higher than that which the lenses
focus through the apertures, thus reducing the beam current by removing
those electrons with energies outside of the nominal and reducing the spot
size by ensuring that those electrons remaining have a smaller spread of
energies.

Having said that, increase the condenser current, reduce the spot size or
reduce the beam current, whichever applies.

If you're new to electron microscopy, there is one thing that really must
be stressed - cleanliness. The smallest, microscopic, piece of dust, lint,
fiber or electrically insulating contamination can wreak havoc inside an
electron optics column. They tend to build up a negative electrical charge
under influence of the beam that can create repulsive fields that deflect
the electron beam and create weird aberrations. Contamination from
fingerprints and solvents is extremely minor compared to the effects of a
single fiber in the electron path.

Lastly, what ultimately determines the resolution possible in an instrument
is the care with which it was installed. That includes the siting of the
instrument in regards to mechanical vibrations in the building as well as
the sources of electromagnetic interference. But most important is the
careful routing of the various wires and tubing within and outside the
instrument. The electron optics column is mounted on a table that is
isolated from vibrations from the frame that holds it. All connections
that run to this table must be carefully routed in order to provide similar
isolation. Of similar concern is the electrical grounding of the various
components of an SEM. Do not assume that because various large metal
components appear to have electrical continuity that they do.
Manufacturers typically provide for individual grounds to many components
for good reason.




Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Tuesday, October 01, 2002 10:51 PM, by way of MicroscopyListserver
[SMTP:little-at-earthtech.org] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (little-at-earthtech.org) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
} October 1, 2002 at 21:27:21
}
---------------------------------------------------------------------------
}
} Email: little-at-earthtech.org
} Name: George Luce and Scott Little
}
} Organization: Earthtech International, Inc.
}
} Education: Graduate College
}
} Location: Austin, Texas
}
} Question: We have recently revived a Cambridge Stereoscan 150 SEM
} from about 1978. The machine is now operational, but we cannot get
} good resolution or focus above a few thousand X magnification.
}
} We would like to communicate with someone familiar with this or a
} similar model, who could give us some guidance on improving the
} resolution. Details of our efforts can be supplied. We are
} technically competent people with backgrounds in physics,
} engineering, high voltage, electronics, vacuum, etc.
}
} Thank you.
}
}
} George Luce (geolucetx-at-aol.com)
}
} Scott Little (little-at-earthtech.org)
}
}
} Earthtech International, Inc.
} Austin, Texas
}
} Phone (512) 342-2185
}
} www.earthtech.org
}
}
---------------------------------------------------------------------------
}



From daemon Wed Oct 2 05:50:11 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 2 Oct 2002 11:42:04 +0100 (GMT Daylight Time)
Subject: Re: Ask-A-Microscopist:ESEM Low Vacuum Mode. how useful is it?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In this case the "bells and whistles" are (still, I
believe) unique as they allow you to look at fully hydrated
specimens and have water in the chamber.

I am a happy XL30 ESEM (tungsten) user. If you can afford
it the FEGESEM has advantages over the tungsten filament
ESEM related to kV, spot size, SE signal and beam damage
for viewing biological samples.

Dave


On Wed, 2 Oct 2002 00:53:13 -0500 by way of
MicroscopyListserver {mgrace-at-fit.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mgrace-at-fit.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
} October 1, 2002 at 15:16:59
} ---------------------------------------------------------------------------
}
} Email: mgrace-at-fit.edu
} Name: Michael Grace
}
} Organization: Florida Institute of Technology
}
} Education: Graduate College
}
} Location: Melbourne, FL, USA
}
} Question: I am working toward aquisition of a new SEM that will see
} use mostly in biological sciences applications, but will also see
} some use from materials scientists, chemists, etc. I am considering
} one of the new ESEMs (specifically the FEI/Philips Quanta 200.
} Question: how useful is the low vacuum mode, esp. for biological
} applications? Also interested in feedback from those with other
} applications, but I mainly want to be sure that the money is spent
} wisely- that we are not getting all the bells and whistles simply
} because they exist.
}
} Thank you.
}
} ---------------------------------------------------------------------------
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Oct 2 08:01:13 2002



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 02 Oct 2002 08:47:06 -0400
Subject: Confocal list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has the confocal listserver been closed or have I been purged? Haven't
gotten anything for a while.
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/EM


From daemon Wed Oct 2 09:33:14 2002



From: Anjeanette Ormonde :      Anjeanette.Ormonde-at-unilever.com
Date: Wed, 2 Oct 2002 09:13:58 -0500 (Central Daylight Time)
Subject: Confocal Microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was hoping someone could recommend some manufacturers of confocal
microscopes.
We are just beginning to investigate the possibility of purchasing one for
our labs and while the literature I've read is intriguing, I would like
more specific information about the various scopes available and what they
can really do for us. Also, I need some idea of the price. I would
appreciate it if any manufacturers or people with personal accolades for a
specific scope would please contact me offline. Thanks for your help!

Angie



From daemon Wed Oct 2 10:55:17 2002



From: Chuck.Butterick-at-degussa.com
Date: Wed, 2 Oct 2002 10:43:21 -0500
Subject: Wood Identification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

Would someone please direct me to some resource, a good text, or better
yet, a web site that can help me identify wood types by either cross-grain
and/or with grain using a microscope? I have some crushed slivers that I'd
like to identify. Any help would be appreciated. Thanks in advance.

Chuck Butterick
Degussa Engineered Carbons
Borger, TX



From daemon Wed Oct 2 11:21:22 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Wed, 2 Oct 2002 09:14:06 -0700 (PDT)
Subject: Re: Ask-A-Microscopist:ESEM Low Vacuum Mode. how useful is it?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a JEOL JSM-5800LV, which is a low vacuum model.
I use the LV feature quite a bit.

The down side is: you must use backscatter detection
in this mode, a slight loss in high-mag resolution,
and cost.

The plus side is convenience - samples don't have to
be coated...they can be imaged as-is. There's no
spurious EDS peaks from conductive coatings, which
makes spectrum interpretation easier. Sample
pump-down is even quicker.

A lot of people are going with this new generation of
SEMs. You still have the option of imaging the
regular way with these instruments.

My application is mostly material science. Being
concentrated in biology, I think you have a stronger
argument for buying an LV SEM. It would see a lot of
use in other disciplines once other people learn of
the instrument's capabilities.

Stu Smalinskas
Metallurgist
SKF
Plymouth, Michigan

__________________________________________________
Do you Yahoo!?
New DSL Internet Access from SBC & Yahoo!
http://sbc.yahoo.com


From daemon Wed Oct 2 11:24:17 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 02 Oct 2002 09:15:53 -0700
Subject: Re: Ask-A-Microscopist:ESEM Low Vacuum Mode. how useful is it?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Michael,
We recently purchased a new SEM and got the variable-pressure option, since
all the manufacturers offer this now and it doesn't cost much more. Ours is
a materials engineering department, but the microscopes are used by all
sorts of university and commercial users, including biologists.
I was pleasantly surprised with how useful the VP is for materials
applications. Samples that are not high vacuum compatible, such as wet or
oily stuff, samples that are impossible to coat or that cannot be coated
because they are needed in their current state for something else or just
things you want to look at very quickly, all work fine in VP mode, either by
back-scattered electron mode or a special secondary electron detector for
variable pressure. Apparently, with a cool or cold stage, the applications
are extended even more. That is next on my wish list. I would not consider
buying a conventional SEM without variable pressure option, just for the
versatility it offers.
I have no experience with the FEI ESEM, which goes to a much higher pressure
than the variable pressure range of my microscope.
At 12:53 AM 10/02/2002 -0500, you wrote:
}
} Email: mgrace-at-fit.edu
} Name: Michael Grace
}
} Organization: Florida Institute of Technology
}
} Education: Graduate College
}
} Location: Melbourne, FL, USA
}
} Question: I am working toward aquisition of a new SEM that will see
} use mostly in biological sciences applications, but will also see
} some use from materials scientists, chemists, etc. I am considering
} one of the new ESEMs (specifically the FEI/Philips Quanta 200.
} Question: how useful is the low vacuum mode, esp. for biological
} applications? Also interested in feedback from those with other
} applications, but I mainly want to be sure that the money is spent
} wisely- that we are not getting all the bells and whistles simply
} because they exist.
}
} Thank you.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Oct 2 12:02:36 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Wed, 2 Oct 2002 11:53:51 -0500
Subject: RE: Ask-A-Microscopist: SEM: Cambridge Stereoscan questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Excellent point.
And just some additional recommendations.

SEM resolution is highly specimen dependant. If you do not
have standard specimens for checking resolution, you can try
(for magnifications up to 10k) fractured metal. Do not use
non-conducting coated specimens.

Use shortest possible working distance.

If you cannot get rid completely of astigmatism with
electronic controls you should clean and/or realign
column.

By the way, what is "a few thousand X magnification"? 10k
could be pretty good for 1978 vintage SEM.

Vladimir


} Yes, but are you experienced in electron microscopes? This
} is definitely a
} field that not only encompasses those mentioned, but uses
} them, as well as
} others, all in synergistic manner.
}
} When it comes to resolution, look to your friend, the
} condenser control (or
} spot size or beam current or whatever else it may be called).
} On that
} vintage of Cambridge, I expect it is called spot size. If
} everything else
} is OK, the condenser is the primary control that determines
} the resolution
} of the instrument.
}
} This is because the primary aberration in an electron optic
} instrument is
} chromatic aberration. That is the result of the wide spread
} in energy
} ranges of the electrons emitted from the electron gun. That
} spread creates
} what is known as a spherical aberration that affects the beam
} in both the X
} and Y directions. As they travel through the various electromagnetic
} lenses of the SEM, electrons of different energies are
} affected differently
} primarily resulting in a different focal point in the Z axis. The
} condenser lens, and associated apertures, are used to reduce
} this energy
} spread.
}
} Here's the deal. The higher the condenser current, the
} smaller the spot
} size and the smaller the beam current. It's hard to explain
} in text, but
} you actually have two condenser lenses. Their purpose is to
} provide a
} de-magnification (reduction in apparent size) of the source of the
} electrons. The source is the tungsten filament, or cathode,
} that emits
} electrons from a rather large area of around 100 microns
} (micrometers).
}
} In the process, there are a couple of imaginary images
} formed, or crossover
} points, where apertures or electron absorbers with small
} central openings
} are located. Electrons of different energies will be
} affected differently
} by the electromagnetic lenses - electrons of lower energy
} will be displaced
} more than those of higher energies. The apertures will capture those
} electrons that have an energy lower or higher than that which
} the lenses
} focus through the apertures, thus reducing the beam current
} by removing
} those electrons with energies outside of the nominal and
} reducing the spot
} size by ensuring that those electrons remaining have a
} smaller spread of
} energies.
}
} Having said that, increase the condenser current, reduce the
} spot size or
} reduce the beam current, whichever applies.
}
} If you're new to electron microscopy, there is one thing that
} really must
} be stressed - cleanliness. The smallest, microscopic, piece
} of dust, lint,
} fiber or electrically insulating contamination can wreak
} havoc inside an
} electron optics column. They tend to build up a negative
} electrical charge
} under influence of the beam that can create repulsive fields
} that deflect
} the electron beam and create weird aberrations. Contamination from
} fingerprints and solvents is extremely minor compared to the
} effects of a
} single fiber in the electron path.
}
} Lastly, what ultimately determines the resolution possible in
} an instrument
} is the care with which it was installed. That includes the
} siting of the
} instrument in regards to mechanical vibrations in the
} building as well as
} the sources of electromagnetic interference. But most
} important is the
} careful routing of the various wires and tubing within and
} outside the
} instrument. The electron optics column is mounted on a table that is
} isolated from vibrations from the frame that holds it. All
} connections
} that run to this table must be carefully routed in order to
} provide similar
} isolation. Of similar concern is the electrical grounding of
} the various
} components of an SEM. Do not assume that because various large metal
} components appear to have electrical continuity that they do.
} Manufacturers typically provide for individual grounds to
} many components
} for good reason.
}
}
}
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} On Tuesday, October 01, 2002 10:51 PM, by way of MicroscopyListserver
} [SMTP:little-at-earthtech.org] wrote:
} }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} ---------.
} }
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (little-at-earthtech.org) from
} } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
} } October 1, 2002 at 21:27:21
} }
} --------------------------------------------------------------
} -------------
} }
} } Email: little-at-earthtech.org
} } Name: George Luce and Scott Little
} }
} } Organization: Earthtech International, Inc.
} }
} } Education: Graduate College
} }
} } Location: Austin, Texas
} }
} } Question: We have recently revived a Cambridge Stereoscan 150 SEM
} } from about 1978. The machine is now operational, but we cannot get
} } good resolution or focus above a few thousand X magnification.
} }
} } We would like to communicate with someone familiar with this or a
} } similar model, who could give us some guidance on improving the
} } resolution. Details of our efforts can be supplied. We are
} } technically competent people with backgrounds in physics,
} } engineering, high voltage, electronics, vacuum, etc.
} }
} } Thank you.
} }
} }
} } George Luce (geolucetx-at-aol.com)
} }
} } Scott Little (little-at-earthtech.org)
} }
} }
} } Earthtech International, Inc.
} } Austin, Texas
} }
} } Phone (512) 342-2185
} }
} } www.earthtech.org
} }
} }
} --------------------------------------------------------------
} -------------
} }
}
}
}


From daemon Wed Oct 2 13:42:36 2002



From: sghoshro-at-NMSU.Edu
Date: Wed, 2 Oct 2002 12:31:22 -0600 (MDT)
Subject: Re: Ask-A-Microscopist:ESEM Low Vacuum Mode. how useful is it?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael,

The low vacuum mode is extremely useful for biological samples. For low
magnification pictures it is extremely useful and these days we hardly use
our sputter coater and critical point dryer, no fixation (you avoid use of
hazardous fixatives in that way). We are very satisfied with the results.
We have a Hitachi 3200N variable pressure SEM.

Good luck,

Soumitra Ghoshroy


*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty Member, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml

On Wed, 2 Oct 2002, by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mgrace-at-fit.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
} October 1, 2002 at 15:16:59
} ---------------------------------------------------------------------------
}
} Email: mgrace-at-fit.edu
} Name: Michael Grace
}
} Organization: Florida Institute of Technology
}
} Education: Graduate College
}
} Location: Melbourne, FL, USA
}
} Question: I am working toward aquisition of a new SEM that will see
} use mostly in biological sciences applications, but will also see
} some use from materials scientists, chemists, etc. I am considering
} one of the new ESEMs (specifically the FEI/Philips Quanta 200.
} Question: how useful is the low vacuum mode, esp. for biological
} applications? Also interested in feedback from those with other
} applications, but I mainly want to be sure that the money is spent
} wisely- that we are not getting all the bells and whistles simply
} because they exist.
}
} Thank you.
}
} ---------------------------------------------------------------------------
}
}



From daemon Wed Oct 2 15:17:10 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Wed, 2 Oct 2002 15:07:48 -0500
Subject: RE: Ask-A-Microscopist:ESEM Low Vacuum Mode. how useful is it?

Contents Retrieved from Microscopy Listserver Archives
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FEI's ESEMs are equipped with good gaseous secondary electron
detectors and for "good" specimens their resolution in wet
mode is pretty close to the resolution in conventional mode.
They are widely used in materials science.

Nevertheless, in biological applications most specimens are
not "good". The best results I have had with hard tissue,
dentin and bone. In wet mode with wet dentin or bone I can
use magnification up to 25k, but if these specimens are dry
then in the same conditions (wet mode) magnification is
limited to 10k. With Au-Pd coated specimens in high vacuum
mode I can use magnifications up to 150k (I have a field
emission ESEM). I have had good results in observation
plants and (surprise!) bugs. Success with cell cultures
was marginal. And I have to mention that application of
EDS analysis in wet mode could be complicated and limited.

However, with all these limitations, I have found that
ESEM is a useful tool in our research.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


} Email: mgrace-at-fit.edu
} Name: Michael Grace
}
} Organization: Florida Institute of Technology
}
} Education: Graduate College
}
} Location: Melbourne, FL, USA
}
} Question: I am working toward aquisition of a new SEM that will see
} use mostly in biological sciences applications, but will also see
} some use from materials scientists, chemists, etc. I am considering
} one of the new ESEMs (specifically the FEI/Philips Quanta 200.
} Question: how useful is the low vacuum mode, esp. for biological
} applications? Also interested in feedback from those with other
} applications, but I mainly want to be sure that the money is spent
} wisely- that we are not getting all the bells and whistles simply
} because they exist.
}
} Thank you.
}
} --------------------------------------------------------------
} -------------
}
}


From daemon Wed Oct 2 15:20:24 2002



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Wed, 2 Oct 2002 15:56:47 -0400
Subject: TEM-SEM: Surplus Kevex Components

Contents Retrieved from Microscopy Listserver Archives
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Good Afternoon,

Following is a description of surplus Kevex equipment. If you are interested
in any of these items and are willing to pay shipping expenses please
contact me.

Regards,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com


Description of Used Kevex Components


1. Kevex DeltaPlus Microanalysis System, Level 5 (without detector)
Serial #5500240-0158
Installed: November 1992
Electronics cabinet houses the signal processing electronics, MCA,
12-slot
dual wide DEC Q-bus card cage with LSI-11/73 minicomputer, dedicated
microprocessor and associated logic circuitry and data storage memory.
Kevex model 4461A pulse processor. Single Syquist drive available.

2. Kevex Delta Analyzer, complete system, however, light element detector
has 'blown' window
Serial #501678-1172
Detector: 30mm square, model 3600-0674
Serial #0790-5803
Pre-amplifier model 2003
Installed: October 1990




From daemon Wed Oct 2 16:48:47 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 02 Oct 2002 17:51:31 -0400
Subject: Re: Wood Identification

Contents Retrieved from Microscopy Listserver Archives
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Chuck,

To be very precise, crushed slivers may not be adequate, since some
distinctions are based on the variations in cell shape that occur through an
annual growth ring. You might want to contact Arno Schniewind :

arnops-at-nature.berkeley.edu

of the Forest Products Laboratory of the University of California, or his
colleagues:

http://www.ucfpl.ucop.edu/

Arno has been very helpful over the years and the lab provides wood
identification services.

John Twilley
Conservation Scientist

Chuck.Butterick-at-degussa.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Listers,
}
} Would someone please direct me to some resource, a good text, or better
} yet, a web site that can help me identify wood types by either cross-grain
} and/or with grain using a microscope? I have some crushed slivers that I'd
} like to identify. Any help would be appreciated. Thanks in advance.
}
} Chuck Butterick
} Degussa Engineered Carbons
} Borger, TX





From daemon Wed Oct 2 18:11:22 2002



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Wed, 02 Oct 2002 19:01:11 -0400
Subject: Thanks for the replies concerning EDS in TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I would like to thank all the people for their helpful suggestions and
knowledge concerning the quantitative composition analysis in a TEM.

I did not give the details clearly when I posted the question.
I'd just like to provide more information on the detector and the
microscope I used.
The microscope is a Jeol-2010 LaB6 (though I wish it was a FEG) operated at
200kV.
The detector is a PGT Ge detector with a ultra thin window. The software is
called imix.
The atomic fraction I got for the EuS is calculated automatically from this
software after putting in voltage, take-off angle, density, and sample
thickness.

Just for your information.

Best Regards
Yan Xin





From daemon Wed Oct 2 20:57:04 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 02 Oct 2002 09:15:53 -0700
Subject: Re: Ask-A-Microscopist:ESEM Low Vacuum Mode. how useful is it?

Contents Retrieved from Microscopy Listserver Archives
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Dear Michael,
We recently purchased a new SEM and got the variable-pressure option, since
all the manufacturers offer this now and it doesn't cost much more. Ours is
a materials engineering department, but the microscopes are used by all
sorts of university and commercial users, including biologists.
I was pleasantly surprised with how useful the VP is for materials
applications. Samples that are not high vacuum compatible, such as wet or
oily stuff, samples that are impossible to coat or that cannot be coated
because they are needed in their current state for something else or just
things you want to look at very quickly, all work fine in VP mode, either by
back-scattered electron mode or a special secondary electron detector for
variable pressure. Apparently, with a cool or cold stage, the applications
are extended even more. That is next on my wish list. I would not consider
buying a conventional SEM without variable pressure option, just for the
versatility it offers.
I have no experience with the FEI ESEM, which goes to a much higher pressure
than the variable pressure range of my microscope.
At 12:53 AM 10/02/2002 -0500, you wrote:
}
} Email: mgrace-at-fit.edu
} Name: Michael Grace
}
} Organization: Florida Institute of Technology
}
} Education: Graduate College
}
} Location: Melbourne, FL, USA
}
} Question: I am working toward aquisition of a new SEM that will see
} use mostly in biological sciences applications, but will also see
} some use from materials scientists, chemists, etc. I am considering
} one of the new ESEMs (specifically the FEI/Philips Quanta 200.
} Question: how useful is the low vacuum mode, esp. for biological
} applications? Also interested in feedback from those with other
} applications, but I mainly want to be sure that the money is spent
} wisely- that we are not getting all the bells and whistles simply
} because they exist.
}
} Thank you.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu Oct 3 07:51:50 2002



From: David Rothbard :      rothbardD-at-netscape.net
Date: Thu, 03 Oct 2002 08:37:39 -0400
Subject: Re: wood identification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here are some texts on wood identification:

Hoadley, R.B. (1990) Identifying Wood: Accurate Results with Simple
Tools. Taunton Books. 1990 (try Amazon.com)
Parham, R. and Gray, R. (1982) The Practical Identification of Wood Pulp
Fibers. TAPPI Press, 212 pp. 1982.(try www.tappi.org)
Strelis, I. and Kennedy, R.W. (1967) Identification of North American
Commercial Pulpwoods and Pulp Fibers. University of Toronto Press. 1967
Core, H.A., Cote, W.A., Day, A. C. (1979) Wood Structure and
Identification. 1979


If you only have slivers, it can be difficult, expecially with
conifers. I can recommend some experienced analysts off-line.


David Rothbard
Earthborn Solutions
rothbardD-at-netscape.net


}
} Would someone please direct me to some resource, a good text, or better
} yet, a web site that can help me identify wood types by either
cross-grain
} and/or with grain using a microscope? I have some crushed slivers
that I'd
} like to identify. Any help would be appreciated. Thanks in advance.
}
} Chuck Butterick
} Degussa Engineered Carbons
} Borger, TX





From daemon Thu Oct 3 07:51:50 2002



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Thu, 3 Oct 2002 08:24:35 -0400
Subject: LM: Liquid Crystals

Contents Retrieved from Microscopy Listserver Archives
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Good morning all,

Could someone recommend a book, journal article or reference on the topic of
liquid crystals that encompasses both an introduction to the topic and
techniques for their characterization by light microscopy. Thanks.

Cheers,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com



From daemon Thu Oct 3 10:26:41 2002



From: Marilyn Levy :      mlevy-at-cellbio.wustl.edu
Date: Thu, 3 Oct 2002 10:10:21 -0500
Subject: TEM(plastic embedding of tendon)

Contents Retrieved from Microscopy Listserver Archives
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I have been asked to process adult mouse tendon, a new tissue for me. I
would appreciate any protocols or suggestions for processing this tissue
from fixation through TEM plastic embedding. Also, should I be concerned
about damage to my diamond knife during thin sectioning? Thanks in advance.
Marilyn Levy


From daemon Thu Oct 3 10:26:41 2002



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Thu, 3 Oct 2002 11:01:53 -0700
Subject: RE: Wood Identification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Would someone please direct me to some resource, a good text, or better
} yet, a web site that can help me identify wood types by either cross-grain
} and/or with grain using a microscope?

R. Bruce Hoadley is an expert on wood identification. If your sample size is
relatively large, i.e., splinter sized, rather than completely crushed, then
you can probably get pretty far just from Hoadley's book _Identifying Wood_
which is still in print (Taunton Press) - about US$30 from Amazon.com. If
not, you might try contacting Dr. Hoadley directly to see what he suggests.

Bruce Girrell






From daemon Thu Oct 3 13:59:24 2002



From: Sales Department :      sales-at-smoking.com.cn
Date: Thu, 3 Oct 2002 08:42:33 +0200
Subject: Discounted Cigs

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir or Madam

In the past you have requested information on discounted products. If you are not a smoker, and find this email offensive, then we sincerely apologise. We will be only too happy to take you off our database.

If you are a smoker, however, you are probably fed up with paying high prices for your cigarettes and tobacco. Take a look at what we can do for you at
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We can send you, legally, by registered air mail, direct to your door, 4 cartons of cigarettes or 40 pouches of rolling tobacco (all brands are available) from only 170 Euros - about 105 pounds - fully inclusive of postage and packing. Why pay more?

If you would rather not hear from us any more, this link will ensure that you are not bothered again.
mailto:unsubscribe-at-smokersassociation.co.uk

Yours faithfully.
Smokers Association

http://www.smokersassociation.co.uk/?S=32&ID=2

xay7712041y

From daemon Thu Oct 3 15:11:14 2002



From: Erik Bodegom :      Bodegom-at-pdx.edu
Date: Thu, 03 Oct 2002 13:02:26 -0700
Subject: position

Contents Retrieved from Microscopy Listserver Archives
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Microscopist and Lab Manager for a Centralized Electron Microscope
Laboratory Facility at Portland State University

Portland State University seeks an electron microscopist to operate and
manage a newly established electron microscopy (EM) facility consisting of a
FEI/Philips (Tecnai F-20) 200kV field emission high-resolution transmission
electron microscope (TEM) equipped with an embedded digital scanning
transmission electron microscopy (STEM) capability, and energy dispersive
x-ray spectrometer (EDS), a JEOL 2000FX TEM, and a 611 FEI focused ion beam
microscope.

PSU is an AA/EO institution and, in keeping with the president's diversity
imitative, welcomes applications from diverse candidates and candidates who
support diversity.

The responsibilities of the position include the management, operation, and
maintenance of the microscopes, and training and assisting faculty, and
student, and outside university users of the microscopes.

Candidates preferentially have at least 5-years experience in managing an
electron microscopy multi-user facility in an academic or industrial
research setting and an outstanding record of team and personal
accomplishments. A publication record of EM-related research is expected.
Degree in electron microscopy and relevant disciplines in using microscopes
is required.Advanced university degree is required as well as a demonstrated
experiences in either materials science, geological, and or biological
materials characterizations are essential. The candidates must have working
knowledge onexperience using the microscopes listed above and are be
familiar such operational techniques as digital image acquisition, image
processing (digital micrograph) , structure simulation, and EDS analysis.

The candidates should also be able to have experience performing minor
microscope repairs and maintenance. repair minor problems of the
microscopes while mMicroscopes will be maintained under ajor components of
the microscopes will be covered by the service contracts.

The successful candidates are expected to have be self-motivationmotivated,
patience, and the ability to work and work well with exiting technical
staff, faculty, and students, and outside users.

Candidates will be selected based on their qualifications and
accomplishments. Salary will be commensurate with qualifications and
experience.

Review of applications will begin on October 1, 2002, and continue until the
position is filled.

Interested candidates, please should send a letter of interest with a
two-page management plan for a multi-user EM facility and a full resume and
arrange to have five letters of reference, sent to the address below:

Search Committee for EM Manager
Physics DepartmentDepartment of Physics
Portland State University
P.O. Box 751
Portland, OR 97207-0751





From daemon Thu Oct 3 22:57:50 2002



From: Sales Department :      sales-at-smoking.com.cn
Date: Thu, 3 Oct 2002 08:42:33 +0200
Subject: Discounted Cigs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Sir or Madam

In the past you have requested information on discounted products. If you are not a smoker, and find this email offensive, then we sincerely apologise. We will be only too happy to take you off our database.

If you are a smoker, however, you are probably fed up with paying high prices for your cigarettes and tobacco. Take a look at what we can do for you at
http://www.smokersassociation.co.uk/?S=32&ID=2

We can send you, legally, by registered air mail, direct to your door, 4 cartons of cigarettes or 40 pouches of rolling tobacco (all brands are available) from only 170 Euros - about 105 pounds - fully inclusive of postage and packing. Why pay more?

If you would rather not hear from us any more, this link will ensure that you are not bothered again.
mailto:unsubscribe-at-smokersassociation.co.uk

Yours faithfully.
Smokers Association

http://www.smokersassociation.co.uk/?S=32&ID=2

xay7712041y

From daemon Fri Oct 4 09:17:23 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Fri, 04 Oct 2002 10:05:31 -0400
Subject: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
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I have been coating samples via electron beam evaporation to a thickness of
20-25 nm as determined by blue interference colors on polished brass.
Recently the mineral guys lost their coater so I have also been coating
their material to the same blue color they said they used as well. They used
resistance heating.

In examining those samples they noticed their EDS/WDS results appearing a
little low so we went ahead and coated the standards as well.

More digging has revealed they coat with graphite rods whereas I have been
using amorphous C rods. They swear the coats are thicker and looking at
theirs-vs-mine it appears so BUT we both evaporated to the same blue color
on brass.

Could there be a difference in the optical properties of the graphite and
amorphous rods?? I assumed maybe incorrectly that the evaporated material
was basically the same from the two rods thus blue brass would also equal
the same thickness. Thanks

Scott Whittaker
Lab Manager
Laboratories of Analytical Biology
Smithsonian Institution
National Museum of Natural History
PO Box 37012 MRC104
Washington DC 20013-7012
202-357-1651



From daemon Fri Oct 4 09:50:44 2002



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Fri, 04 Oct 2002 10:39:36 -0400
Subject: PanaCL and/or cold stage

Contents Retrieved from Microscopy Listserver Archives
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Hi group - anybody out there currently using a CL detector with a cold
stage - please email me ASAP
Thanks so much
Barb



From daemon Fri Oct 4 10:07:47 2002



From: S. Kuehner :      kuehner-at-u.washington.edu
Date: Fri, 4 Oct 2002 08:00:34 -0700 (PDT)
Subject: Re: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
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I hope someone knows the answer to this question because I've wondered the
same thing... carbon rods vs graphite. another scott

************************************************
....amphiboles do violence to history...
T. Feininger, 2001. (taken out of context)
****************************

Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************

On Fri, 4 Oct 2002, Scott Whittaker wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have been coating samples via electron beam evaporation to a thickness of
} 20-25 nm as determined by blue interference colors on polished brass.
} Recently the mineral guys lost their coater so I have also been coating
} their material to the same blue color they said they used as well. They used
} resistance heating.
}
} In examining those samples they noticed their EDS/WDS results appearing a
} little low so we went ahead and coated the standards as well.
}
} More digging has revealed they coat with graphite rods whereas I have been
} using amorphous C rods. They swear the coats are thicker and looking at
} theirs-vs-mine it appears so BUT we both evaporated to the same blue color
} on brass.
}
} Could there be a difference in the optical properties of the graphite and
} amorphous rods?? I assumed maybe incorrectly that the evaporated material
} was basically the same from the two rods thus blue brass would also equal
} the same thickness. Thanks
}
} Scott Whittaker
} Lab Manager
} Laboratories of Analytical Biology
} Smithsonian Institution
} National Museum of Natural History
} PO Box 37012 MRC104
} Washington DC 20013-7012
} 202-357-1651
}
}
}



From daemon Fri Oct 4 10:44:40 2002



From: Jiao, Chengge :      chengge.jiao-at-uk.feico.com
Date: Fri, 4 Oct 2002 17:29:21 +0200
Subject: A SIMS book

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

Does anybody know where I can buy a book called "Secondary Ion Mass Spectrometry: Basic concepts, Instrumental aspects, applications and trends " by Benninghoven, A.; F.G.Rudenauer F.G., and Werner H.W.

Thanks for your information.

Chengge Jiao


From daemon Fri Oct 4 14:58:23 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 04 Oct 2002 13:11:11 -0700
Subject: Re: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
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I used to use carbon/graphite rods for making thin carbon film. I do find
that using thermal evaporation, the properties of the film originated from
graphite or "carbon" are different. It looks like, "graphite" films are
more fragile, less stable and somehow more "crumbly". I do notice that
"graphite" evaporates at lower current (thermal) also. This difference (in
properties of the film) is less pronounced when Electron Beam Evaporator
(Electron Gun) was used... But, still, I prefer to use spectrographic
quality "carbon rods".

In general, carbon thickness is difficult to determine precisely. I am
using Maxtec TM-450(?) thickness monitor with preciseness 0.01 nm. Even
with this instrument, the real thickness may vary up to 10-15% (never
measured exact value, but have "feelings"). Sergey


At 08:00 AM 10/4/02 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Fri Oct 4 14:58:23 2002



From: Mark Aindow :      m.aindow-at-uconn.edu
Date: Fri, 4 Oct 2002 15:51:28 -0400
Subject: Postdoctoral Position

Contents Retrieved from Microscopy Listserver Archives
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University of Connecticut
Institute of Materials Science

Postdoctoral Position
Displacive Phase Transformations

A post-doctoral position is available immediately within the Institute
of Materials Science at the University of Connecticut to work on
displacive phase transformations in crystalline solids. The systems to
be studied include metallic pseudoelastic alloys and ferroelectric thin
films. The ideal candidate will have experience in both
microstructural characterization (TEM, SEM etc.) and
phenomenological/crystallographic modeling of phase transformations.
This position is a one-year appointment, with possible renewal for a
second year.

To apply, please send a complete resume, together with a list of
publications and contact details for 3 referees to either Prof. S.
Pamir Alpay (p.alpay-at-ims.uconn.edu) or Prof. M. Aindow
(m.aindow-at-uconn.edu). Screening of applications will begin immediately,
and will continue until the position is filled. We encourage
applications from under-represented groups, including minorities, women
and people with disabilities.


*********************************************************

Mark Aindow, Associate Professor,
Department of Metallurgy and Materials Engineering
Institute of Materials Science,
97 North Eagleville Road, Unit 3136
University of Connecticut, Storrs,
CT 06269-3136, USA

Tel: +1 (860) 486-2644
FAX: +1 (860) 486-4745
Email: m.aindow-at-uconn.edu

**********************************************************



From daemon Fri Oct 4 15:02:12 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 4 Oct 2002 14:56:06 -0500
Subject: CSMMS Meeting Announcement (Call for Papers)

Contents Retrieved from Microscopy Listserver Archives
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FIRST CALL FOR PRESENTATIONS & FALL MEETING ANNOUNCEMENT

SOCIETY: Central States Microscopy and Microanalysis Society (CSMMS)

DATE: November 15, 2002. From 9 AM to 4 PM.

LOCATION: Giant City State Park. Carbondale, Illinois

DESCRIPTION:

The CSMMS will hold its Fall meeting at the rustic lodge at the Giant
City State Park in Carbondale, Illinois. Located in a lovely wooded
park, the lodge will serve as the focal point for the presentations
and luncheon banquet. The registration and orientation will begin at
9 AM and presentations will commence at 9:30 AM.

The first half-day will involve research presentations, followed by a
luncheon and business meeting. The second half-day will involve
technical presentations, demonstrations and discussions. For example,
we will have presentations on digital imaging (Production of Large
Format Posters, Digital Publishing, etc.) as well as discussions of
useful techniques and technical pointers. Topics may cover any type
of microscopy and microanalysis.

If you are interested in attending or presenting, please contact me
for details.

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
##############################################################









From daemon Fri Oct 4 20:55:18 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 04 Oct 2002 18:47:26 -0700
Subject: Re: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
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Tobias, good question

In specification it indicates 0.01 nm accuracy as far as I remember, but as
I mentioned in my original posting, it seems to me that I do have
approximately 10% difference in the film thickness even with that smart
thickness monitor. In general, I am happy with that instrument. It's very
solid and sensitive enough in 10-20 A scale. Sergey

At 04:15 PM 10/4/02, you wrote:
} Dear Sergey,
} Does your thickness monitor really measure down to 0.01 nm
} accuracy?? That's amazing.
}
} Tobias
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Oct 4 22:05:21 2002



From: Johnson Mulete :      john_2007-at-cheerful.com
Date: Sat, 05 Oct 2002 04:56:06
Subject: Private Proposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Re: Business Proposal

Sir/Madam,

I am JOHNSON MULETE JR. the son of Mr. Luke Radebe MULETE from Zimbabwe. I am sorry this mail
Will Surprise you, though we do not know, I got your contact Through South African Chamber of Commerce.

During the current war against white farmers in Zimbabwe and the support of President Robert Mugabe to
Claim all the white owned farms in our country to gain Favor for re-election. All white farmers were asked to
Surrender their farms to the government for Re-distribution and infact to his political party
Members and my father though black, was the treasury Of the farmers association and a strong member of an
Opposition party that did not support the presidents Idea. He then ordered his party members and the police
Under his pay role to invade my fathers farm and burn Down everything in the farm. They killed my
Father and took away a lot of items from his farm.

After the death of my father, our local pastor and a Close friend of my father handed us over will
Documents with instructions from my father that we Should leave Zimbabwe for South Africa incase any
Thing happen to him, process the release of the fund Kept in custody for us in a security company unknown
To the company that the content is money hence it was deposited as Personal Belongings and Ensure that we do not remain there as we could Easily be found by his enemies. The total amount is US$21.5M.We are therefore soliciting for
Your assistance to help us move the fund out of South Africa, as our fate and future is far from Reality, Hence this mail to you. The president's present ban of International Press into Zimbabwe is just a few of the Unthinkable things he is committing in my Country.

I have tried to reach my father's close friend Mr. John Casabas from Australia also a farmer who was
Leaving in Zimbabwe with us but left with his family Late last year following this ugly development to no Avail.

Should you be interested to help us, contact me Immediately via email for easy communication and I Will furnish you with the time frame and modalities of The transaction. Please note that this transaction is} 100% confidential and risk free and will not endanger You or us in any way. We have resolved to give you 20% Of the total sum upon confirmation of the fund in any Account of your choice were the incident of taxation Will not take much tool on the money and we look Forward to coming over to your country to invest our Share and settle there. I will provide you my phone number
As soon as you indicate your willingness to assist us so that our conversation can be 100%confidential.

Please do not use the reply button, reply only to(johnmulte-at-netscape.net} Please take note.

God bless you indeed as you help yourself and us.

MR. Johnson Mulete Jr.



From daemon Sat Oct 5 09:26:29 2002



From: charles j day :      wa5ekh-at-juno.com
Date: Fri, 4 Oct 2002 09:03:28 -0500
Subject: Re: Image Analysis Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ANYBODY perfected the SEM "shallow and deep(bottom and no bottom-black)
hole area(morphometry) size analysis??
I'm still having trouble with thresholding, flatness, edge defintion and
mostly repeatability and accuracy.
+_20-50% isn't of much use. Manual edge detection is too slow and not
much more repeatable. I'd like to see at least +_5%.
jeffrey day/mesqite(o), texas


From daemon Sat Oct 5 11:00:25 2002



From: Cle02JS12-at-aol.com (by way of Ask-A-Microscopist)
Date: Sat, 5 Oct 2002 10:47:38 -0500
Subject: Ask-A-Microscopist:LM American Optical ? Microscope repair parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Cle02JS12-at-aol.com) from on Saturday, October 5, 2002
at 01:33:09
---------------------------------------------------------------------------

Email: Cle02JS12-at-aol.com
Name: Sandy

Education: 9-12th Grade High School

Location: Washington State

Question: Need referral on how/where to find replacement part for an
older college level microscope. Emblem indicates "Alpha
Omega-Spencer".
Under side indicates American Optical Co.

Any leads much appreciated.

Thank You



---------------------------------------------------------------------------


From daemon Sat Oct 5 15:21:04 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sat, 5 Oct 2002 14:03:59 -0600
Subject: Re: Image Analysis Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeffrey,

I am not quite sure what the problem is that you are referring to, but you
may want to check out our ACT module (check our web site). It is designed to
find edges and do geometric measurements on those edges, using tools
developed for the semiconductor industry. If you want to send me an image
for a trial run, please send it to my address below.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: charles j day [mailto:wa5ekh-at-juno.com]
Sent: Friday, October 04, 2002 8:03 AM
To: DrJohnRuss-at-aol.com-at-sparc5.microscopy.com
Cc: microscopy-at-sparc5.microscopy.com; NIH-IMAGE-at-LIST.NIH.GOV


ANYBODY perfected the SEM "shallow and deep(bottom and no bottom-black)
hole area(morphometry) size analysis??
I'm still having trouble with thresholding, flatness, edge defintion and
mostly repeatability and accuracy.
+_20-50% isn't of much use. Manual edge detection is too slow and not
much more repeatable. I'd like to see at least +_5%.
jeffrey
day/mesqite(o), texas


From daemon Sat Oct 5 15:21:04 2002



From: Jim Quinn :      jquinn-at-www.matscieng.sunysb.edu
Date: Sat, 5 Oct 2002 16:10:40 -0400
Subject: Re: A SIMS book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Cheng-Ge Jiao

try "www.google.com"
enter "Benninghoven Rudenauer"
brings up this URL:
http://www.addall.com/Browse/Detail/0471010561.html
states:

Secondary Ion Mass Spectrometry Basic Concepts, Instrumental Aspects, Applications & Trends
A. Benninghoven , H. W. Werner , F. G. Rudenauer
Binding: Hardcover, 1 edition, 1264 pages
Publisher: Wiley, John and Sons, Incorporated
Published Date: 01/01/1987
List: USD $395.00
ISBN: 0471010561


"click on" next link for list of locations:

http://www.addall.com/New/BrowseCompare.cgi?isbn=0471010561

good luck,

Jim

} From Microscopy-request-at-sparc5.microscopy.com Fri Oct 4 20:06:03 2002
} Subject: A SIMS book
} Date: Fri, 4 Oct 2002 17:29:21 +0200
} From: "Jiao, Chengge" {chengge.jiao-at-uk.feico.com}
} To: "Microscopy-at-MSA.Microscopy.Com" {Microscopy-at-sparc5.microscopy.com}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} Does anybody know where I can buy a book called
} "Secondary Ion Mass Spectrometry: Basic concepts,
} Instrumental aspects, applications and trends "
} by Benninghoven, A.; F.G.Rudenauer F.G., and Werner H.W.
}
} Thanks for your information.
}
} Chengge Jiao
}


From daemon Sun Oct 6 09:15:47 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 06 Oct 2002 10:06:14 -0500
Subject: Carbon vs. graphite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

There have been several postings on the subject of (real) carbon vs.
graphite rods for use in carbon coating in a vacuum evaporator.

In many instances, I find that our customers are purchasing "carbon" rods
but which are really graphite rods. I am not suggesting that those who
offer the product as being truly a carbon rod are doing something wrong
because it seems like when we use the words "carbon rods" we don't really
think of it as being carbon or graphite but somehow we know what we all mean
when we say we use "carbon rods".

But graphite (when used as a "carbon rod") is described in terms of its
porosity; indeed, there is a wide range of densities of graphite rods. It
is our perception, but not as a result of exhaustive testing, that the
higher the porosity ( and lower the density), when one evaporates, there is
increased "sparking". I think I would be safe in saying that the
"conventional wisdom" suggests that this is undesirable. At the wholesale
level (e.g. the large quantities that someone like SPI Supplies purchases)
there is in fact a substantial difference in the price between graphite rods
of higher porosity vs. lower porosity. We believe that the lower porosity
(and accompanying higher density) results in less sparking, perhaps less
contaminating nanotubes (which are a bi-product of this kind of evaporation)
and other artifactual features.

Actual "carbon" rods have the lowest porosity. Although we have not tested
it to the point that I would stake my life on it, real carbon rods seem to
spark a whole lot less (if at all) and the final film seems to be much more
featureless, but we think that is due to the reduced amount of "sparking".
Further information can be found at URL
http://www.2spi.com/catalog/spec_prep/carbon-graphite-rods.html

I would be most appreciative of any comments pro or con relative to this
analysis. I have been operating on the assumption that this is more or less
correct. If there are other points of view, I would be the first to want to
know what they are.

Disclaimer: SPI Supplies has offered for some time both carbon rods as
carbon rods and graphite rods as graphite rods.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================





-----------------------------------------------------------------------
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I used to use carbon/graphite rods for making thin carbon film. I do find
that using thermal evaporation, the properties of the film originated from
graphite or "carbon" are different. It looks like, "graphite" films are
more fragile, less stable and somehow more "crumbly". I do notice that
"graphite" evaporates at lower current (thermal) also. This difference (in
properties of the film) is less pronounced when Electron Beam Evaporator
(Electron Gun) was used... But, still, I prefer to use spectrographic
quality "carbon rods".

In general, carbon thickness is difficult to determine precisely. I am
using Maxtec TM-450(?) thickness monitor with preciseness 0.01 nm. Even
with this instrument, the real thickness may vary up to 10-15% (never
measured exact value, but have "feelings"). Sergey


At 08:00 AM 10/4/02 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu





From daemon Sun Oct 6 14:51:27 2002



From: charles j day :      wa5ekh-at-juno.com (by way of MicroscopyListserver)
Date: Sun, 6 Oct 2002 14:34:17 -0500
Subject: SEM Image Analysis-deep and shallow hole sizing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ANYBODY perfected the SEM "shallow and deep(bottom and no bottom-black)
hole area(morphometry) size analysis??
I'm still having trouble with thresholding, flatness, edge defintion and
mostly repeatability and accuracy., etc.
+_20-50% isn't of much use. Manual edge detection is too slow and not
much more repeatable. I'd like to see at least +_5%.
jeffrey
day/mesqite(o), texas


From daemon Sun Oct 6 15:05:30 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 06 Oct 2002 13:22:20 -0700
Subject: Re: Carbon vs. graphite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Charles

Graphite and carbon rods I have (from Ted Pella, I believe) are easily
distinguished: graphite is grayish and carbon is deep black. There is
also difference in hardness: carbon is harder. I really like your point
that graphite produce more "features" when evaporated. It support my
intuitional decision to use "carbon". It seems to me it produces more
uniform film... Thanks. Sergey

At 10:06 AM 10/6/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Oct 6 17:24:47 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 07 Oct 2002 11:12:30 +1200
Subject: Re: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} } } "crumbly". I do notice that "graphite" evaporates at lower current
} } } (thermal) also. This difference (in properties of the film) is less
} } } pronounced when Electron Beam Evaporator (Electron Gun) was used...
} } } But, still, I prefer to use spectrographic quality "carbon rods".
} } }


That's funny. When I inadvertantly changed over from 'carbon' to
'graphite, I found that the graphite needed so much current that the rod
holder on my Edwards 306 became very hot. I couldn't easily get
sufficient evaporation, even at maximum current, so I switched back to
carbon.

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sun Oct 6 19:45:04 2002



From: admin5217q00-at-easymoney.fr.com
Date: Mon, 07 Oct 2002 10:03:03 -1100
Subject: Welcome To the Club

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello!

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==================================================
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==================================================
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===WHEN YOU PLACE YOUR ORDER, MAKE SURE ===
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Remember, this method has been tested, and if you
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==================================================
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==================================================

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==================================================

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==================================================

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Notes: Always send $5 cash (U.S. CURRENCY or EURO) for
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==================================================
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______________________________________________________
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_______________________________________________________

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______________________________________________________
$$$$$$$$$ YOUR SUCCESS GUIDELINES $$$$$$$$$$$

Follow these guidelines to guarantee your success:

=== If you do not receive at least 10 orders for
Report #1 within 2 weeks, continue sending e-mails
until you do.

=== After you have received 10 orders, 2 to 3 weeks
after that you should receive 100 orders or more for
REPORT # 2. If you do not, continue advertising or
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If you have any questions of the legality of this
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* This message is not intended for residents in the
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6997UQjg3-669fCJn5819fHns7-296kNJT5019vwoU0-971KltO6313sGjr5-646xnHV8706VlTT1-476vl77


From daemon Sun Oct 6 19:58:22 2002



From: arrobert-at-mtu.edu (by way of Ask-A-Microscopist)
Date: Sun, 6 Oct 2002 19:53:39 -0500
Subject: Ask-A-Microscopist:TEM Specimen Prep Help...Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (arrobert-at-mtu.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
October 6, 2002 at 19:39:15
---------------------------------------------------------------------------

Email: arrobert-at-mtu.edu
Name: Amy Roberts

Organization: Michigan Technological University

Education: Undergraduate College

Location: Houghton, Mi United States of America

Question: I am in search of specimen preparation procedures for
transmission electron microscopy for a gram negative and a gram
positive bacteria. Could anyone point me to a journal or publication
with this information?

---------------------------------------------------------------------------


From daemon Mon Oct 7 07:19:02 2002



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Mon, 7 Oct 2002 08:08:28 -0400
Subject: Sorvell MT2-B Ultra microtome manual request.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I would like to ask if anyone could fax me a copy of the operating instructions for a
Sorvell MT2-B Ultra microtome.
It is motorized if that matters.

This is for a lady that is not on the list and that works at another research center.

Thank you in advance for your help and time.

724-325-5105 My FAX

Sincerely,

Paul Beauregard
Senior Research Associate
PPG Industries
Monroeville Technical Center
Electron Microscopy
440 College Park Drive
Monroeville, PA 15146

} To calculate the size of one micron in ANY microscopic photograph, one only has to know the magnification. Take the mag and convert it to KX. That number is the number of millimeters that represents one micrometer on your photo. For example, 81385X = 81.385KX and 1micron is 81.385 mm. 516X = 0.516KX and one micron is .516 mm. 1,300,000X = 1300KX and 1300 mm is 1 Micron or 130MMs is 0.1 microns or 13 mm is 10NMs or 100Å.
}
}
}


From daemon Mon Oct 7 08:12:12 2002



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Mon, 7 Oct 2002 10:33:52 -0230
Subject: RE: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott writes ...

} I have been coating samples via electron beam evaporation to a
} thickness of
} 20-25 nm as determined by blue interference colors on polished brass.
} Recently the mineral guys lost their coater so I have also been coating
} their material to the same blue color they said they used as
} well. They used
} resistance heating.
}
} In examining those samples they noticed their EDS/WDS results appearing a
} little low so we went ahead and coated the standards as well.
}
} More digging has revealed they coat with graphite rods whereas I have been
} using amorphous C rods. They swear the coats are thicker and looking at
} theirs-vs-mine it appears so BUT we both evaporated to the same blue color
} on brass.
}
} Could there be a difference in the optical properties of the graphite and
} amorphous rods??

I can't imagine any difference once the carbon has been evaporated ...
i.e., there may be a difference with respect to resistance and temperature
of evaporation (sublimation?) between vitreous carbon and graphite, but once
the transformation happens, carbon is carbon.

I happen to use the red-to-blue transistion, because it is very
distinguishable color change and should result in a coating thickness of
22nm +- 1nm.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)




From daemon Mon Oct 7 08:29:28 2002



From: Rik Brydson :      mtlrmdb-at-leeds.ac.uk (by way of MicroscopyListserver)
Date: Mon, 7 Oct 2002 08:23:19 -0500
Subject: Electron Microscopy and Analysis Conference 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


***************************************************************
First Announcement

Electron Microscopy and Analysis Conference 2003
The University of Oxford
3 - 5 September 2003

Organised by the Electron Microscopy and Analysis Group of the Institute of
Physics
Co-sponsored by the Royal Microscopical Society

Scientific Content
Electron Microscopy is going through the most revolutionary period since its
invention, with leaps in performance of sources, imaging lenses and
spectrometers all at roughly the same time. Scanning probe techniques are
also maturing into useful analytical tools. This is an ideal time for the
microscopy community to appraise when, how and why we use different
techniques to gain different scientific insights. The conference will
address all aspects of electron and scanning probe microscopy and
associated spectroscopy including:

o New Instrumentation - including Aberration Correction and
Monochromation
o New Information from New Instrumentation - Applications
Highlighting Novel Techniques
o Advances in Nanoanalysis - EDX, PEELS and Other Analytical
Techniques
o State-of-the-art SEM, Scanning Probe Microscopy and
Surface Science
o High-resolution Electron Microscopy and Electron
Crystallography
o Advances in Sample Preparation

Applications of microscopy and related techniques will be divided into
sessions according to demand.
These will include experiments in:

o Biogenic Materials
o Catalysis
o Ceramics and Electroceramics
o Composites
o Environmental Samples
o Ferrous and Non-Ferrous Metals
o Fullerenes, Hard Carbon and Related Materials
o Intermetallics
o Interfaces and Surfaces
o Magnetic Materials
o Sensor Materials
o Semiconductors
oSuperconductors

Sessions of oral and poster presentations will be arranged, and, where
possible, associated with invited
keynote speakers. Papers from post-graduate students are particularly
encouraged; student bursaries
will be available through the Institute of Physics EMAG Group.

Advanced School
The Advanced School provides an opportunity for postgraduate students,
research assistants and others with an interest to learn about a range of
advanced imaging techniques in electron and related microscopies techniques
from experts in the field. The school will involve a combination of tutorial
lectures and hands-on demonstrations in the Department of Materials, using
the wide range of sophisticated instrumentation available there. The
lecturers/demonstrators will be leading exponents in the techniques
concerned. There will be considerable opportunity for interaction. To
maximise the effectiveness of the School, numbers will be limited to about
20.

Trade Exhibition
Accompanying the conference there will be a trade exhibition where delegates
and visitors will be able to see and discuss the latest products and
developments.

Social Programme
The social programme will include a welcome reception, an Exhibitor Buffet
and a conference dinner. Further details will be available in due course.

Conference Venue
The scientific sessions, the Exhibition and the poster presentations will
take place at the Oxford University Examination Schools. For more
information about how to get to Oxford please visit the Oxford
University web site at http://www.ox.ac.uk/aboutoxford/how.shtml

Important Dates
January 2003 all for Papers
21 March 2003 Deadline for Abstract Submission
May 2003 Registration Mailing
18 July 2003 Deadline for Registration


Organising Committee

EMAG Chairman R M D Brydson
Local Committee Chairman A Petford-Long/J Hutchison
Programme Organiser A Bleloch
Proceedings Editor S McVitie
Exhibition Organiser R Doole
Advanced School Organiser D J Cockayne

General Enquiries
Jasmina Bolfek-Radovani, The Institute of Physics, 76 Portland Place, London
W1B 1NT
Tel +44 (0)20 7470 4800 Fax: +44 (0)20 7470 4900
Email: conferences-at-iop.org
http://physics.iop.org/IOP/Confs/EMG/

Exhibition Enquiries
Ron Doole, EMAG03, Department of Materials, University of Oxford, Parks
Road, Oxford., OX1 3PH
Tel: + 44 (0)1865 273701 Fax: + 44 (0)1865 283333
Email:ron.doole-at-materials.oxford.ac.uk


Media Registration
Accredited journalists are welcome to attend all or part of this meeting
free-of-charge. To qualify for free
registration please contact Alice Bows, Press Officer, Public Affairs
Department, Tel: +44 (0)20 7470 4800, Fax: +44 (0)20 7470 4848, Email:
alice.bows-at-iop.org.



From daemon Mon Oct 7 09:04:55 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 7 Oct 2002 10:03:28 -0400
Subject: Re: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey & Co.;

In an analysis I did several years ago I wanted to place a controlled amount
of carbon on a surface and measure the ultimate strength of a gold to gold
weld in a microcircuit. We had great difficulty with this since we had no
method to know, with any accuracy, the real deposition thickness. Our
target was 100 angstroms, with increments up to 500. We attempted to use
Auger, however, with a carbon species and the fact that the first 50
angstroms in the Auger survey are thrown out, we never actually determined
what the final result was. Would AFM have been a better choice for this
measurement?

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Friday, October 04, 2002 9:47 PM
To: Microscopy-at-sparc5.microscopy.com


Tobias, good question

In specification it indicates 0.01 nm accuracy as far as I remember, but as
I mentioned in my original posting, it seems to me that I do have
approximately 10% difference in the film thickness even with that smart
thickness monitor. In general, I am happy with that instrument. It's very
solid and sensitive enough in 10-20 A scale. Sergey

At 04:15 PM 10/4/02, you wrote:
} Dear Sergey,
} Does your thickness monitor really measure down to 0.01 nm
} accuracy?? That's amazing.
}
} Tobias
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Oct 7 10:06:56 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Mon, 07 Oct 2002 10:49:57 -0400
Subject: NESM Fall Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




To all:

The Fall meeting of NESM (New England Society for Microscopy) will be held
on Tuesday, October 15th at M.I.T. in Cambridge, MA.

The program will be devoted to Raman Spectroscopy, and the schedule is as
follows:

5:30 - 6:00pm Registration
6:00 - 7:15pm Buffet Supper
7:15 - 8:45pm Technical sessions

7:15pm - Raman Microspectroscopy and Imaging
David S. Himmelsbach, United States Department of Agriculture Agricultural
Research Service
Richard B. Russell Research Center, Athens, Georgia, 30677

8:00pm - Raman Spectroscopy on Isolated Carbon Nanotubes
Shin Grace Chou, Center for Materials Science and Engineering,
Massachusetts Institute of Technology, Cambridge, MA 02139


Place: Stratton Student Center (Building W20), Twenty Chimneys Lounge
(3rd. floor), 84 Massachusetts Avenue, Cambridge.

Travel: A map and directions are on the Web at
http://whereis.mit.edu/bin/map?state=0&pri.x=281&pri.y=123
Parking will be extremely difficult because of on-going road construction
on Vassar Street (the main location of on-street parking). If you park in
any MIT lot, even in the evening, you will be ticketed unless you have an
MIT sticker. There is a public lot on the corner of Vassar St. and Mass.
Ave. that may be available. There is a public garage behind the Legal
Seafoods Restaurant in Kendall Square (10 mins. walk). There is another
garage several blocks North just off Mass. Ave. (2 blocks beyond the NECCO
building). It should also be possible to find on-street parking, but be
prepared to walk.

If possible, we recommend you use public transport. The number 1 bus from
Harvard to Dudley stops on Mass Ave outside the student center. The Red
Line stops at Central and Kendall are each 10 minutes walk (use Central if
you come from Harvard or Alewife, Kendall if you come from Boston or points
South.)

Registration for members is $5.00 and for non-members is $20.00 (this
includes a one-year membership in NESM). The deadline for registration is
Friday, October 11th. Please contact Paul Bain, NESM Treasurer,
(Paul_Bain-at-hms.harvard.edu) if you are planning to attend.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Mon Oct 7 11:20:03 2002



From: Jinguo Wang :      jqw11-at-psu.edu
Date: Mon, 07 Oct 2002 12:10:08 -0400
Subject: LN2 and/or heating holder for a 2000FX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Fellows,

We are interested in buying a used 2000FX LN2 and/or heating holder, If
anyone would like to sell those used holders please let me know.

Thanks,

Jinguo Wang, Ph.D
The Pennsylvania State University
Materials Research Institute
194 Materials Research Institute Building
University Park, PA 16802
Tel: (814) 865-9285
Fax: (814) 863-8561, (814) 863-0637
email: jqw11-at-psu.edu



From daemon Mon Oct 7 12:56:36 2002



From: JHoffpa464-at-aol.com
Date: Mon, 07 Oct 2002 13:49:15 -0400
Subject: Re: Sorvell MT2-B Ultra microtome manual request.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


mt-2s are so easy, why would she need a manual?


From daemon Mon Oct 7 13:23:04 2002



From: Marcia A. Miller, Ph.D. ABMM :      Marcia-at-dbts.uicomp.uic.edu
Date: Mon, 7 Oct 2002 13:21:23 -0500
Subject: TEM: Fusarium & Penicillium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use a great deal of carbon and graphite daily for making our substrates.
Some in our lab prefer graphite, some carbon.
If you examine the crystalline structure you'll find carbon is more random
(less crystalline). Graphite requires more current in the Ladd evaporators.
Carbon is more expensive.
We offer carbon and high quality graphite. Based on our experience and
customer feedback we are of the opinion that excellent results can be
attained with carbon or high quality graphite.

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
To: "Sergey Ryazantsev" {sryazant-at-ucla.edu} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, October 06, 2002 7:12 PM



Ladies/Gentlemen:

I am searching for TEMs of two fungi; one is our newly
discovered Penicillium that is related to several listed below.
Need TEM's with good ultrastructure and labeling of
internal structures. Will appreciate help by listing references,
text books, personal electronmicrographs, etc. Need information
for publication purposes.

1) Fusarium verticilloides (moniliforme) or related species.

2) Penicillium species related to new species identified:

a. Penicillium restrictum
b. Penicillium dimorphosporum
c. Penicillium striatisporum

If TEM's of above Penicillium species not available,
then electronmicrographs of monoverticillate, nonvesiculate
penicilli with short stipes.

Thank you.

e-mail: mam-at-uic.edu






From daemon Mon Oct 7 13:29:04 2002



From: Nancy.P.Piatczyc-at-williams.edu
Date: Mon, 07 Oct 2002 14:23:53 -0400
Subject: mass spec upgrade

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear list,

This is off-topic, many apologies, but I am stumped. I am not familiar
with mass spectrometers, but have been asked to get information on
upgrading a 1070's vintage mass spec - quadrupole. The manufacturer is
Nuclide. They want to send the detector output to a PC. Many thanks
for any info from vendors or individuals. Nancy

Nancy Piatczyc
Bronfman Science Center
Williams College
Williamstown, MA 01267






From daemon Mon Oct 7 14:03:35 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 07 Oct 2002 11:54:19 -0700
Subject: RE: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter

Actually, only one direct way to measure REAL thickness I do know: it's
embedding in the plastic and cross-sectioning strongly PERPENDICULAR. All
other ways are arbitrary. As I mentioned in original report, I do have 10%
difference from experiment to experiment for carbon. Heavier elements may
be measured, perhaps, more precisely... Sergey

At 07:03 AM 10/7/02, you wrote:
} Sergey & Co.;
}
} In an analysis I did several years ago I wanted to place a controlled amount
} of carbon on a surface and measure the ultimate strength of a gold to gold
} weld in a microcircuit. We had great difficulty with this since we had no
} method to know, with any accuracy, the real deposition thickness. Our
} target was 100 angstroms, with increments up to 500. We attempted to use
} Auger, however, with a carbon species and the fact that the first 50
} angstroms in the Auger survey are thrown out, we never actually determined
} what the final result was. Would AFM have been a better choice for this
} measurement?
}
} Peter Tomic
} Anadigics, Inc.
}
} -----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Friday, October 04, 2002 9:47 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: SEM Carbon Coating thickness
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Oct 7 14:37:23 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 7 Oct 2002 15:36:14 -0400
Subject: RE: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey;

Even with a great FESEM as we have, I would be hard pressed to image 100
angstroms of anything with a 10 angstrom accuracy. The FESEM sometimes can
do 15 angstroms on an ideal sample on a good day, but I could never resolve
10A/100A. By "perpendicular" x-sectioning, are you referring to mount lap
and polish, microtome? Also, what is a "Smart Thickness Monitor" you mention
below? Maybe I need one. In short, how does it do the measurement, a stylus
like AFM, optical method?

Regards,

Peter

Ps. I would imagine the Los Angeles smog would put 100 angstroms of soot
down on a glass slide in short order, unlike pristine New Jersey.

-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Monday, October 07, 2002 2:54 PM
To: Peter Tomic; Microscopy-at-sparc5.microscopy.com

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu




From daemon Mon Oct 7 14:53:44 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Mon, 07 Oct 2002 15:42:03 -0400
Subject: Carbon vs graphite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ok here's a new twist. It was kinda slow Friday and we ended up running
several different rods and simulated samples again coated to blue on brass.
My brass washer was the same color as theirs. The consensous upstairs is
still that that my coatings are substantially thicker and looking at the
samples I agree they are darker.

They evaporated carbon and sometimes graphite via resistance. I use e-beam
evaporation, and graphite rods take about 5 minutes, heating the head to
cherry in color so is not really an option. Could the different thickness be
due to different density of the films? The e-beam evaporated material in
theory has a finer more uniform structure with less space, thus the film is
more "dense" There would be more carbon in the film per unit thickness so
when I coat to the blue 25nm, more carbon is present.

Resistance evaporation is less controlled and there is less carbon in the
film structure, but still colors brass blue at 25nm.

The other option is that a denser film optically reflects differently and
the blue is something like 40nm or so. I had ruled that out since I have a
thickness monitor and it is reading correctly.

Anyway, just some more thoughts to toss around. I never really paid any
attention to the carbon films and have already learned much from the
discussion so far. Thanks


From daemon Mon Oct 7 15:01:55 2002



From: Nancy.P.Piatczyc-at-williams.edu
Date: Mon, 07 Oct 2002 15:55:39 -0400
Subject: mass spec correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Opppps, typo, it's a 1970's vintage that needs an upgrade. Nancy




From daemon Mon Oct 7 15:31:33 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 07 Oct 2002 15:21:48 -0500
Subject: RE: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What is the actual molecular structure of an "amorphous" carbon rod?
Is it really amorphous? Microcrystalline? Something else?
Given the crystal form of graphite, I suspect that it is a much more
efficient generator of bucky balls, nanotubes, and suchlike
structures. This could explain the problems with film thickness and
consistency from graphite evaporation vs (amorphous) carbon rods. How
would the bucky balls, nanotubes, etc. affect the electrical
properties of a carbon film, and thus its behavior in an electron
beam?
Or is this all wrong and irrelevant?

Has anyone done any AFM/STM on carbon films deposited by these
different methods?

Phil

} Scott writes ...
}
} } I have been coating samples via electron beam evaporation to a
} } thickness of
} } 20-25 nm as determined by blue interference colors on polished brass.
} } Recently the mineral guys lost their coater so I have also been coating
} } their material to the same blue color they said they used as
} } well. They used
} } resistance heating.
} }
} } In examining those samples they noticed their EDS/WDS results appearing a
} } little low so we went ahead and coated the standards as well.
} }
} } More digging has revealed they coat with graphite rods whereas I have been
} } using amorphous C rods. They swear the coats are thicker and looking at
} } theirs-vs-mine it appears so BUT we both evaporated to the same blue color
} } on brass.
} }
} } Could there be a difference in the optical properties of the graphite and
} } amorphous rods??
}
} I can't imagine any difference once the carbon has been evaporated ...
} i.e., there may be a difference with respect to resistance and temperature
} of evaporation (sublimation?) between vitreous carbon and graphite, but once
} the transformation happens, carbon is carbon.
}
} I happen to use the red-to-blue transistion, because it is very
} distinguishable color change and should result in a coating thickness of
} 22nm +- 1nm.
}
} cheerios ... shAf :o)
} Avalon Peninsula, Newfoundland
} www.micro-investigations.com (in progress)

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Mon Oct 7 18:41:23 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 07 Oct 2002 16:30:37 -0700
Subject: RE: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
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When carbon (or any other material) evaporated (sublimated for carbon?) as
I understand, it's not mono-atomic "vapor". It contains atomic clusters of
different size. Those clusters may be produced during "evaporation" or
later in the "vapor" clouds. I could imagine that evaporation conditions,
the density of cloud etc may shift local conditions in the favor of some
carbon state. Condensation conditions on the surface may also vary
(temperature etc). So, to me it's easy to imagine how it happens that
carbon film is heterogeneous (mica steps could initiate some specific
condensation). I was thinking to do AFM on different "carbons". It should
be done in the vacuum environment I guess. Unfortunately, I don't have
access to such instrumentation. Sergey

At 01:21 PM 10/7/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Oct 8 07:15:01 2002



From: Tony Bruton :      Bruton-at-nu.ac.za
Date: Tue, 08 Oct 2002 14:04:48 +0200
Subject: Oil mist problem with JEOL 100CX

Contents Retrieved from Microscopy Listserver Archives
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Greetings All

I have been told that the problem we are currently experiencing with
our ~ 1980 JEOL 100CX has been seen before.

We would very much appreciate any information or advice.

We are getting an accumulation of oil mist (DP oil ?) on the inside of
the viewing chamber glass in the specimen chamber of our 100CX. Where is
it coming from, where do I start looking for the fault ? We no sooner
clean it off than it is back again.

Look forward to hearing from listers.

Tony

Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
Mob. 082 782 9640
website: www.nu.ac.za/microscopy.asp
Email: bruton-at-nu.ac.za
Postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa


From daemon Tue Oct 8 07:46:54 2002



From: Robert Wieland :      wieland-at-me.udel.edu
Date: Tue, 8 Oct 2002 08:38:57 -0400 (EDT)
Subject: Re: SEM Carbon Coating thickness

Contents Retrieved from Microscopy Listserver Archives
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Excuse me if this is common knowledge...
Thirty years ago, the organization I worked for was fabricating carbon
"bottles" to contain cadmium sulphide to be heated for vacuum deposition.
We ran into the problem that commercial "graphite" contains several
percent boron, put in to make the stuff machinable. Since we didn't want
boron involved in our process, we had to specify "nuclear-grade" graphite,
intended for reactor parts where they didn't want any boron, either.
This turned out to be hard enough to get that we laid in a ten-year supply
when we finally found some.
Are "graphite" evaporator rods boron-free? If not, could that be
responsible for the differences reported? I assume amorphous carbon is
pure.



Robert Wieland wieland-at-me.udel.edu
The very concept of human governance is a moral dilemma:
If the people are good, it is a mistake to create authorities over them;
If they are not good, it is a mistake to create authorities out of them.

You can easily peg people by listening to them recount a hand:
winners talk about how they played the cards they were dealt;
non-winners, about how they should have played the cards they were dealt;
and losers, about the cards they should have been dealt.

R F Wieland wieland-at-me.udel.edu 39.68N 75.74W
Icom R75 Heathkit GR-81 Inverted-L in the attic



From daemon Tue Oct 8 09:12:10 2002



From: Kathryn Zeisler-Mashl :      kzeisler-at-wpi.edu
Date: Tue, 8 Oct 2002 10:11:28 -0400
Subject: TEM - 100C double tilt holder

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
We are looking for a double tilt (tilt-tilt) holder for a Jeol 100C/100CX to
examine metallurgical specimens. If you have one available, please reply to
me directly (kzeisler-at-wpi.edu).

Thank you very much.
Kate Zeisler-Mashl

-----------------------------------------
Kathryn L. Zeisler-Mashl, Ph.D.
Research Assistant Professor
Materials Science and Engineering Program
Worcester Polytechnic Institute
100 Institute Road, Worcester, MA 01609
T: (508)831-6025 F: (508)831-5178
kzeisler-at-wpi.edu





From daemon Tue Oct 8 09:16:41 2002



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Tue, 08 Oct 2002 11:09:43 -0300 (ADT)
Subject: EM job classificcation

Contents Retrieved from Microscopy Listserver Archives
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Hi Listservers!
I have a few questions that I would like to ask you If you feel you
know the answer please reply. My university together with local
union reclassified staff positions according to Aiken plan. Needless
to say my position was downgraded.
My questions are:
1. Can EM supervisory position be compared to any on campus
staff positions (university has Department of Sciences and
Veterinary Medecine)? I supervise small multi--user lab, we have 2
TEM, and we do research, diagnostics, teaching (under- and
graduate level) in the field of biological sciences. No fancy staff like
X-ray analysis or cryo.
2. What in your opinion is a necessary minimum experience
required to perform this job ? I am talking about accepting the
position and immediately getting into the stream of work.
3. What in your opinion is the minimum level of education that is
required to perform this job?

You can reply directly to me. Thanks in advance
Frustrated
Dorota


From daemon Tue Oct 8 10:19:20 2002



From: Drew Price :      dprice2-at-sigecom.net
Date: Tue, 8 Oct 2002 11:00:43 -0500
Subject: McCrone Research Institute

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Boron is in the manufacturing process. It's probably in the PPB range in
the graphite rods. Some graphite rods may be boron free but you'd have to
assume there is some boron in most graphite rods.

Disclaimer: Ladd sells a wide variety of electron microscope related
products.

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Robert Wieland" {wieland-at-ME.UDel.Edu}
To: "John Arnott" {ladres-at-worldnet.att.net}
Cc: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, October 08, 2002 8:38 AM


I am scheduled to attend McCrone's applied polarized light microscopy course
later this month. I have a medical background consisting of gross human
anatomy and physiology, a limited science background, law enforcement and
legal experience, but I currently do not work in a science field. A friend
of mine recently provided me with some "hands-on" with a polarized light
microscope.

How else can I prepare for this course of instruction? I am paying for this
training myself, and I want to get the most out of it.

Any input or advice would be greatly appreciated.

Thank you,

Drew Price
dprice2-at-sigecom.net




From daemon Tue Oct 8 12:38:05 2002



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Tue, 08 Oct 2002 13:44:37 -0400
Subject: turbo pump carbon coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

More on the discussion on carbon/graphite rods used in EM labs for
evaporation:

To understand what the situation really is, you have to go back to the
"starting" material, which differs between the different manufacturers of
the final product. The starting material could be either coke, coal, or
what is called "pitch". Since this can come from different geographical
locations, the level of impurities varies, including the level of boron
which is everywhere.

The first step is the converstion of the pitch to carbon and then the second
step is the conversion of the carbon to graphite. At least that is the
process used in the production of the SPI Supplies offered rods. The
problem arises because boron very easily forms a carbide and that carbide is
very difficult to purify out of the final product.

In years gone by, and before analytical instrumentation had the low
detection limits of today's instrumentation, it was indeed common to talk
about carbon or graphite as being "boron free". Of course, nothing is
ever zero, we can only talk about detection limits and say that if present,
it is less than some amount.

The instrumentation used for the control of boron in the SPI Supplies carbon
and graphite rod products has a detection limit of slightly more than 1 ppm
and we keep our product to a boron level of less than 2 ppm.

The purification cost is a major cost element of the delivery of high purity
carbon or graphite rods; not all applications require this kind of purity.
The way products are presented, either in catalogs or websites, might
suggest they are all the same but they are not all the same. I can not
address the purity of the carbon or graphite rods sold by others, only that
offered by SPI Supplies. But certainly this level of purity of boron is
well beyond that needed by anyone using these products in an EM lab
application.

} From our experience, the biggest reason why different rods from different
sources evaporate differently is due to differences in the density of the
grahpite rods.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================





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Boron is in the manufacturing process. It's probably in the PPB range in
the graphite rods. Some graphite rods may be boron free but you'd have to
assume there is some boron in most graphite rods.

Disclaimer: Ladd sells a wide variety of electron microscope related
products.

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Robert Wieland" {wieland-at-ME.UDel.Edu}
To: "John Arnott" {ladres-at-worldnet.att.net}
Cc: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, October 08, 2002 8:38 AM


Hi group - anybody out there that currently uses a carbon coater with a
turbo pump? Looks like our Pelco carbon coater died and parts are hard
to find.
Can any of you recommend a carbon coater that you are happy with?
Thanks
Barb



From daemon Tue Oct 8 12:54:20 2002



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Tue, 08 Oct 2002 13:45:49 -0400
Subject: cold stage and SEM

Contents Retrieved from Microscopy Listserver Archives
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Hi didn't really get a response from my last query - anybody currently
using a CL with SEM? We are looking to upgrade our system and get a cold
stage.
Thanks
Barb



From daemon Tue Oct 8 13:28:19 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 08 Oct 2002 17:20:24 -0400
Subject: Re: McCrone Research Institute

Contents Retrieved from Microscopy Listserver Archives
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Hi Tony,

This problem might be caused by several conditions. First, determine what
oil is
present in the projection chamber. Possibilities are: Santovac (DP),
Hydrocarbon (PVP), and air compressor oil, which could be either Hydrocarbon
or an Ester, depending on compressor type. The latter might be irrelevant in
case that you have replaced original JEOL compressor with a dry compressor,
however: an old compressor oil could be present in the pneumatic
lines/valves for years, and might eventually show up on the vacuum side of
pneumatic valves upon moveable seal(s) failure.

I use a highly volatile solvent (such as Pentane), others may work too.
Pentane dissolves Hydrocarbon oil very well and fast, Santovac very poorly,
and Ester oil to some degree. In addition, compressor oil (especially one
sitting in the lines and valves for years, and especially Ester oil) has
deep red/brown color, clearly visible even in microscopic droplets.

Remove a small polished metal part with oil mist visible on it from the
contaminated area. Observe oil mist under stereo microscope, see the color,
try consistency of the droplets with the needle. Santovac is much more
viscous, especially in a cool room. Rinse that part briefly in
Pentane, see if some droplets have disappeared, or were reduced in size more
than others.

Many scenarios are possible, including following:
a) ODP(s) cooling failed (clogged water lines) - Santovac in the
column/viewing chamber. Likely scenario.

b) Pneumatically actuated vacuum valves acting slowly (damage, dirt,
deformed moving seals, compressor oil and water in pneumatic cylinders)- PVP
Hydrocarbon oil or/and Santovac are blown into vacuum when valves switching
for venting or rough pumping is delayed. Likely scenario.

c) Damaged seals in the pneumatically actuated vacuum valves- compressor oil
in the column/viewing chamber. BTW, do you drain oil and water from air
compressor tank regularly? Do you
have water mist filter installed between compressor and a TEM? Likely
scenario.

d) Damaged PVP- Hydrocarbon oil makes it's way into the ODP, and from there
into column/viewing chamber. Unlikely scenario.

e) Pirani gauges or circuits are defective- vacuum sequences are out of
order. Connect stand-alone vacuum gauge to a TEM (multiple ports) and verify
switching points/thresholds. Unlikely scenario.

Unfortunately, an easy fix is unlikely for a problem like this. I recommend
spending some time and conduct an investigation, with vacuum system diagram
in mind. This will help to trace problem more accurately, and avoid
unnecessary disassembly.

Possible remedies may include cleaning/re-packing pneumatically actuated
vacuum valves, cleaning pneumatic lines, cleaning an ODP and replacing the
Santovac, replacing worn out air compressor, replacing/servicing PVP.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: Tony Bruton {Bruton-at-nu.ac.za}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, October 08, 2002 8:04 AM


There is an excellent book on the subject by F. Donald Bloss called "Optical
Crystallography" and it is available from the Minerological Society of
america for about $25.00.

Don
----- Original Message -----
} From: "Drew Price" {dprice2-at-sigecom.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, October 08, 2002 12:00 PM


Why not ask the people who run the course?

Geoff

Drew Price wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am scheduled to attend McCrone's applied polarized light microscopy course
} later this month. I have a medical background consisting of gross human
} anatomy and physiology, a limited science background, law enforcement and
} legal experience, but I currently do not work in a science field. A friend
} of mine recently provided me with some "hands-on" with a polarized light
} microscope.
}
} How else can I prepare for this course of instruction? I am paying for this
} training myself, and I want to get the most out of it.
}
} Any input or advice would be greatly appreciated.
}
} Thank you,
}
} Drew Price
} dprice2-at-sigecom.net

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Oct 8 18:33:06 2002



From: HIS HIGHNESS WALID S. AL SAID :      walid_al1-at-mail.com
Date: Wed, 09 Oct 2002 00:23:21
Subject: CLAIMS/INVESTMENTS FROM HIS HIGHNESS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am HIS HIGHNESS WALID SULMAN AL SAID, Emir of the state of
Bahrain.

I got to know you through a foreign trade office in London
during my official trip last year.

I am making this contact to you to inquire from you if you can
assist me manage some investment for me in your
country.Actually all I want from you is to be my manager
abroad.I would like you to invest in Properties(Real Estate)
and company shares and other lucrative ventures of your
interest for me.

I have Forty Five Million United States Dollars
(US$45,000,000.00)into a VAULT with a Security Finance Company
abroad,but due to many commitments I can not travel out of my
domain for the claim,hence I contacted you to assist me make
the claim on my behalf.

I will provide you with all the necessary documents to enable
you represent me as my business manager to make the claim by
signing the necessary document for onward transfer of the money
into your account which you will use for the investment.

If you agree to assist me by representing me make the claim
with the Security Company, I will now contact the Security
Finance Company to inform them about your person as my
business manager, that they should give you the necessary
attention needed.

For your assistance in this transaction I will offer you 10% of
the total amount, I have also set aside 2% for expenses, while
88% left will be invested into buying of Properties (Real
Estate) company shares and other profit oriented ventures. I
have taken this decision to invest abroad due to restriction
over currency out flow in my country.

Upon your Acceptance I will require the following from you:

1. A letter of Assurance that you will invest and manage wisely
on my behalf.

2. Your telephone and fax numbers to enable the Security
Finance Company communicate with you for the claim. Your
mobile telephone will be of great assistance as well.

For security reasons, I would want every communication between
us in this transaction be confidential. Always reach me via E-
mail for I may not be chanced to speak with you on telephone.

In the later days we will be able to meet for us to discuss
issues of mutual understanding in respect to our investment
project.

Looking forward to a fruitful business relationship with you.

Best regards,

His Highness,
WALID SULMAN AL SAID(Emir of the State of Bahrain).




From daemon Tue Oct 8 20:20:52 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Fri, 8 Nov 2002 20:09:10 -0600
Subject: High Speed Video Camera

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,

I have limited experience with video recording so I may not be giving enough
information; also limited for other reasons. I am in the market for a high
speed video camera and would like some mfg recomendations. We would like to
record a process that takes about 1 second and view an area that is about
1cm square and have a depth of field about 1cm. In other words, the process
takes place within a one cubic cm volume. Also want a camera that can
record without using strobe illumination. Reasonably high resolution (for
HS Video) 800 by 600 would be nice but probably way too expensive. Maybe 2k
frames per second? Would also perfer something that is "somewhat" portable.
Definitely plan to try it out before purchase.

Any recomendations and information in selecting such an instrument would be
very appreciated.

Damian Neuberger
Baxter Healthcare Corp.
damian_neuberger-at-baxter.com



From daemon Tue Oct 8 20:49:29 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Mon, 07 Oct 2002 18:42:21 -0400
Subject: Re: mass spec upgrade

Contents Retrieved from Microscopy Listserver Archives
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-------- Original Message --------






From daemon Tue Oct 8 20:49:29 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Tue, 08 Oct 2002 18:12:30 -0400
Subject: Re: Oil mist problem with JEOL 100CX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Original Message --------






From daemon Tue Oct 8 21:15:23 2002



From: Bob Roberts :      bobrobs-at-cox.net
Date: Tue, 8 Oct 2002 19:07:30 -0700
Subject: Oil mist problem with the JEOL JEM-100CX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tony,

You have a vacuum leak in the camera chamber and the mist you see is
back-streamed diffusion pump oil. Either the high vacuum valve that isolates
the diffusion pump from the camera chamber when venting and exchanging films
is not closing completely (or has a bad "o" ring seal) OR the chamber door
"o" ring is not sealed and you have a steady stream of oil while pumping the
chamber and under high vacuum pumping.

By the way, there is/was a thin coating of carbon on the inside surface of
the leaded viewing glass. I don't know how many times you've cleaned this
off but you may have to recoat it in a high vacuum carbon evaporator.

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St
Topeka, Kansas 66617-1780
785.246.1232
www.emlabservices.com


Greetings All

I have been told that the problem we are currently experiencing with
our ~ 1980 JEOL 100CX has been seen before.

We would very much appreciate any information or advice.

We are getting an accumulation of oil mist (DP oil ?) on the inside of
the viewing chamber glass in the specimen chamber of our 100CX. Where is
it coming from, where do I start looking for the fault ? We no sooner
clean it off than it is back again.

Look forward to hearing from listers.

Tony





From daemon Tue Oct 8 22:41:53 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 08 Oct 2002 20:34:51 -0700
Subject: Fwd: CLAIMS/INVESTMENTS FROM HIS HIGHNESS

Contents Retrieved from Microscopy Listserver Archives
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Well, so much for your perfect spam filter setup.

How can his highness make it through this low level
filter scheme??

gg


} From: "HIS HIGHNESS WALID S. AL SAID" {walid_al1-at-mail.com}
} Date: Wed, 09 Oct 2002 00:23:21
} To: MicroscopyFilteredEmail1-at-sparc5.microscopy.com
} Subject: CLAIMS/INVESTMENTS FROM HIS HIGHNESS
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Oct 9 03:04:44 2002



From: Arthur Day :      ard-at-ansto.gov.au
Date: Wed, 9 Oct 2002 17:54:38 +1000
Subject: Re: Oil mist problem with JEOL 100CX]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Tony,
} I can't say that I'm familiar with the 100CX vacuum system, but I
} recently had a similar problem with a couple of JSM-840s.
}


}
} The second was not so bad, but also having to do with the airlock.
} If the airlock is left open (not pumped) most of the time then the
} airlock vent valve (a small solenoid valve) gets quite hot after a
} while and remains hot (sometimes for years on end). This hardens
} the rubber insert in the plunger so that it will no longer seal
} properly. JEOL operates their airlock on a timer rather than from
} Pirrani readings, so when it times out it says you can transfer the
} specimen. This can lead to a considerable gas pulse and some minor
} backstreaming from the DP before the high vacuum valve closes.
}

Greetings colleagues,

We had precisely the same problem with our JSM-6400 some years ago,
that is, the rubber seal on the end of the plunger in the airlock
vent valve went hard after only a couple of years of operation and
wouldn't seal properly anymore because of the heat from the solenoid
valve. After replacing this we decided to avoid a repeat of the
problem by keeping the airlock under vacuum, thus maintaining the
airlock vent valve in the closed state (solenoid not activated), and
therefore cool for years on end and not hot all of the time. This has
had the added advantage of keeping the inside of the airlock a lot
cleaner and "drier" which should further soften the burden on the
diff pumps when opening it to the main chamber...

This has really cleaned things up for us with no more problems for
over 10 years now, and none ever on our ("similar") newer JSM-6300.


Arthur






Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/


From daemon Wed Oct 9 09:14:05 2002



From: Kent Susan-G10900 :      Susan.Kent-at-motorola.com
Date: Wed, 9 Oct 2002 08:57:56 -0500
Subject: McCrone Applied Polarized Light Microscopy Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Price,

I attended the PLM class at the McCrone Research Institute in Chicago IL USA
in 1990. I don't know how much, if any, it has changed since then but I
found it to be an extremely informative class. It was a very intensive week
of lectures, hands-on laboratory sessions, and evening homework
assignments. Their teaching staff has a genuine interest in microscopy and
was eager to share their knowledge and enthusiasm with the students. The
fact that you're spending your own money to pay for it, and also that you
have such a diverse educational background tells me that you're very
motivated to study and should therefore have little or no trouble with it.

If you can continue to have access to your friend's polarized light
microscope after completing the class, I strongly recommend doing so.
Polarized light microscopy is not only a science but an art, and like
similar subjects, requires a lot of practice to maintain and improve one's
skills.

Best regards,
Sue Kent
Senior Staff Failure Analysis Engineer
Motorola
4000 Commercial Avenue
Northbrook IL USA 60062
847-480-6834


{ { { {I am scheduled to attend McCrone's applied polarized light microscopy
course
later this month. I have a medical background consisting of gross human
anatomy and physiology, a limited science background, law enforcement and
legal experience, but I currently do not work in a science field. A friend
of mine recently provided me with some "hands-on" with a polarized light
microscope.

How else can I prepare for this course of instruction? I am paying for this
training myself, and I want to get the most out of it.

Any input or advice would be greatly appreciated.

Thank you,

Drew Price
dprice2-at-sigecom.net} } } } }




From daemon Wed Oct 9 09:14:05 2002



From: Hendricks, Gregory :      Gregory.Hendricks-at-umassmed.edu
Date: Wed, 9 Oct 2002 09:55:42 -0400
Subject: RE: Seeking used SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a reasonably late model (5-10 years old) SEM for sale.
Please respond to this e-mail address. Sincerely

Greg Hendricks
Core EM Facility Manager
University of Massachusetts Medical School
Worcester, MA

Gregory.Hendricks-at-umassmed.edu




From daemon Wed Oct 9 09:42:16 2002



From: Jim Haley :      haley-at-mvia.com
Date: Wed, 09 Oct 2002 10:33:59 -0400
Subject: Re: High Speed Video Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Damian,

We design custom high speed image recording systems for the biological,
defense, and industrial applications. The software is very easy to
use. We have various configurations depending on requirements for
resolution, frame rate, and cost.

I'd really need some more information from you such as the MINIMUM
acceptable and IDEAL value as well for:
1.) Horizontal and vertical resolution (either in terms of number of
pixels per inch or overall)
2.) Frame rate - is 2000 a minimum?
3.) Record time - is 1 second a maximum for each experiment?
4.) How many record sessions would you like to be able to store before
archiving previous sessions?

Lighting is always a major consideration when doing high speed imaging
as well. Bear in mind that at 2,000 frames per second the camera will
only have 1/66th of the amount of time normally used by standard RS-170
video to allow photons to be collected by the sensor. Instead of the 33
milliseconds exposure time RS-170 video uses, at 2,000 frames per second
we'll only be able to gather light for 0.5 milliseconds. This is one of
the major considerations when determining the frame rate that should be
used for acquisition.

Give me a call or feel free to email me to discuss your application in
greater detail.

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
125 Sherwood Drive
Monaca, PA 15061
voice: (724) 728-7493
fax: (412) 291-1709
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

Damian Neuberger wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Listers,
}
} I have limited experience with video recording so I may not be giving enough
} information; also limited for other reasons. I am in the market for a high
} speed video camera and would like some mfg recomendations. We would like to
} record a process that takes about 1 second and view an area that is about
} 1cm square and have a depth of field about 1cm. In other words, the process
} takes place within a one cubic cm volume. Also want a camera that can
} record without using strobe illumination. Reasonably high resolution (for
} HS Video) 800 by 600 would be nice but probably way too expensive. Maybe 2k
} frames per second? Would also perfer something that is "somewhat" portable.
} Definitely plan to try it out before purchase.
}
} Any recomendations and information in selecting such an instrument would be
} very appreciated.
}
} Damian Neuberger
} Baxter Healthcare Corp.
} damian_neuberger-at-baxter.com


From daemon Wed Oct 9 11:02:36 2002



From: ENGR. JOE UDAH :      joeudah123-at-dpr-nig.com
Date: Wed, 9 Oct 2002 16:49:06 +0100
Subject: DPR

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



DEPARTMENT OF PETROLEUM RESOURCES
PLOT 225 KOFO ABAYOMI STREET VICTORIA ISLAND,LAGOS, NIGERIA.
DIRECT TEL: 234 1 7591519 / FAX: 234 1 7590904
ATTENTION: THE PRESIDENT/C.E.O
RE: URGENT & CONFIDENTIAL BUSINESS PROPOSAL

Dear Sir,

I am ENGR. JOE UDAH ,member committee of the above department
Terms of Reference
My term of reference involves the award of contracts to multinational companies.
My office is saddled with the responsibility of contract award, screening, categorization and prioritization of projects embarked upon by Department of Petroleum Resources (DPR) as well as feasibility studies for selected projects and supervising the project consultants involved. A breakdown of the fiscal expenditure by this office as at the end of last fiscal quarter of 2000 indicates that DPR paid out a whooping sum of US$736M(Seven Hundred And Thirty Six Million, United States Dollars) to successful contract beneficiaries. The DPR is now compiling beneficiaries to be paid for the third Quarter of 2002.
The crux of this letter is that the finance/contract department of the DPR deliberately over –invoiced the contract value of the various contracts awarded. In the course of disbursements, this department has been able to accumulate the sum of US$38.2M(Thirty-eight Million, two hundred Thousand U.S Dollars) as the over-invoiced sum. This money is currently in a suspense account of the DPR account with the Debt Reconciliation Committee (DRC). We now seek to process the transfer of this fund officially as contract payment to you as a foreign contractor, who will be fronting for us as the beneficiary of the fund. In this way we can facilitate these funds into your nominated account for possible investment abroad. We are not allowed as a matter of government policy to operate any foreign account to transfer this fund into.
However, for your involvement in assisting us with this transfer into your nominated account we have evolved a sharing formula as follows:
(1) 20% for you as the foreign partner
(2) 75% for I and my colleagues

(3) 5% will be set aside to defray all incidental expenses both Locally and Internationally during the course of this transaction.

We shall be relying on your advice as regard investment of our share in any business in your country. Be informed that this business is genuine and 100% safe considering the high-power government officials involved. Send your private fax/telephone numbers. Upon your response we shall provide you with further information on the procedures. Feel free to send response by Fax or TEL; expecting your response urgently. All enquiries should be directed to the undersigned by FAX OR PHONE.
Looking forward to a good business relationship with you.

Sincerely,
ENGR. JOE UDAH.




From daemon Wed Oct 9 11:26:26 2002



From: Ann-Fook Yang :      yanga-at-agr.gc.ca
Date: Wed, 09 Oct 2002 11:05:53 -0400
Subject: ESEM for untreated bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been asked to provide a protocol for observing untreated bacteria in ESEM. Can anyone help?


AnnFook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Room 2091, Bldg. 20,
Central Experimental Farm,
Ottawa, Ontario
Canada K1A 0C6

Tel: 1-613-759-1638
Fax: 1-613-759-1701

e-mail: yanga-at-em.agr.ca

} } } Letizia Anello {anello-at-ibs.pa.cnr.it} 10/03/02 08:50AM } } }
Dear Ann Fook,

I am a molecular biologist and have no notion on
electron microscopy and technical details; so I have collaboration with
a chemical engineer of Palermo University. We have tried to examine
unsuccessfully cultured bacteria directly under low vacuum SEM and ESEM
(can you tell me the enlargement and pressure value to use?). I'd prefer
observe in ESEM, completely untreated bacterial samples. Can you send me
a detail protocol? Can I have any photo of yours of bacterial samples in
SEM and ESEM? Thanks, bye.

Letizia

=====================================







From daemon Wed Oct 9 11:26:26 2002



From: Ted Pella :      ted_pella-at-tedpella.com
Date: Wed, 9 Oct 2002 09:20:16 -0700
Subject: Cressington service in the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Listservers:

Following through on a message posted on the Listserver yesterday, we are
clarifying service questions regarding
Cressington equipment as well as OEM Cressington equipment under the PELCO
name.

Please be assured that Cressington vacuum equipment service and parts for
the Americas are available from Ted Pella, Inc. and PELCO International.

This includes versions of the 108 series (plain, /A, /C, /SE), 208 high
resolution series (/HR, /C), 308 series (plain, /R)
and the older versions under the PELCO CC7A, SC-6, SC-7 names.

Our specialist for operation and other questions for the Cressington
equipment can be reached through our office.

You'll find us at 1-800-237-3526, web or e-mail.

Ted Pella




From daemon Wed Oct 9 12:55:03 2002



From: Jay Campbell :      jmcampbe-at-facstaff.wisc.edu
Date: Wed, 09 Oct 2002 13:38:48 -0500
Subject: Re: Fwd: CLAIMS/INVESTMENTS FROM HIS HIGHNESS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------- Forwarded message follows -------
} From: Marcia A. Miller, Ph.D. ABMM {Marcia-at-dbts.uicomp.uic.edu}
To: Microscopy-at-MSA.Microscopy.Com


Spam? It sounded like a pretty good deal to me. I sent His Highness
my contact info along with bank account and credit card numbers. I
can't wait to start pulling down those 10% commissions!
Jay

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--


From daemon Wed Oct 9 13:50:30 2002



From: Shanling Shi :      Shanling.Shi-at-unilever.com
Date: Wed, 9 Oct 2002 14:42:45 -0400
Subject: Cressington service in the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What about Cressington freeze fracture Unit? I was told that Ted Pella could
not yet do the service for freeze fracture in USA. Is it still true? Thanks.

Shanling

Shanling Shi
Advanced Imaging & Measurement
Unilever Research US
45 River Road
Edgewater, NJ 07020
201-840-2340
Shanling.Shi-at-unilever.com


-----Original Message-----
} From: Ted Pella [SMTP:ted_pella-at-tedpella.com]
Sent: Wednesday, October 09, 2002 12:20 PM
To: microscopy-at-sparc5.microscopy.com



Listservers:

Following through on a message posted on the Listserver yesterday, we are
clarifying service questions regarding
Cressington equipment as well as OEM Cressington equipment under the PELCO
name.

Please be assured that Cressington vacuum equipment service and parts for
the Americas are available from Ted Pella, Inc. and PELCO International.

This includes versions of the 108 series (plain, /A, /C, /SE), 208 high
resolution series (/HR, /C), 308 series (plain, /R)
and the older versions under the PELCO CC7A, SC-6, SC-7 names.

Our specialist for operation and other questions for the Cressington
equipment can be reached through our office.

You'll find us at 1-800-237-3526, web or e-mail.

Ted Pella






From daemon Wed Oct 9 14:14:12 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 09 Oct 2002 12:07:03 -0700
Subject: Re: Fwd: CLAIMS/INVESTMENTS FROM HIS HIGHNESS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Great...here's another one then that you should surely
check out:

MRS.THADDEE KABILA" {bhg777-at-tur.net}

She is:

I AM FORTY-NINE YEARS OLD WIDOW (THE THIRD WIFE) OF THE LATE PRESIDENT
LAURENT KABILA OF THE DEMOCRATIC REPUBLIC OF CONGO

I assume she means that he was late for dinner because
I don't recall the Congo having a president. By the way,
tur.net is in Istanbul, Turkey. The poor widow claims to
be in the U.S. with lots of money.

Same old scams......

gary


At 11:38 AM 10/9/2002, you wrote:
} Spam? It sounded like a pretty good deal to me. I sent His Highness my
} contact info along with bank account and credit card numbers. I can't
} wait to start pulling down those 10% commissions!
} Jay
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Oct 9 14:14:12 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 10 Oct 2002 08:01:26 +1200
Subject: JEOL 840A airlock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ken


}
} The second was not so bad, but also having to do with the airlock. If
} the airlock is left open (not pumped) most of the time then the
} airlock vent valve (a small solenoid valve) gets quite hot after a
} while and remains hot (sometimes for years on end). This hardens the
} rubber insert in the plunger so that it will no longer seal properly.
} JEOL operates their airlock on a timer rather than from Pirrani
} readings, so when it times out it says you can transfer the specimen.
} This can lead to a considerable gas pulse and some minor
} backstreaming from the DP before the high vacuum valve closes.
}

Yeah, my 840A has that condition. I put three manual ball-type valves
between the airlock and a spare rotary pump, with a T/C vacuum
gauge in there too, as it seemed like I was going to have to do a lot of
dismantling to fix that valve. The manual system works so well, I find
that 10 minutes sucking on samples before insertion really reduces the
degradation of chamber vacuum on opening the gate valve, that I've
left it like that.

But maybe I should fix the vent valve anyway. Anybody got any tips on
how that get at the vent valve with the minimum of collateral
disassembly?

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Oct 9 16:13:48 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Wed, 9 Oct 2002 15:57:22 -0500
Subject: View Petrographic Slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,


Looking for a method of projecting a petrographic thin section (27X46mm) onto a large screen to be viewed by students. Have tried to sandwich the slide between two pieces of polarized film and display on a microfiche reader and that works pretty well but would like a larger brighter image.

Have tried a digital camera on a petro microscope and again works well but area of view is limited.

Any ideas?

Roy Beavers
Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, Tx. 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-mail.smu.edu



From daemon Wed Oct 9 17:43:48 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Wed, 09 Oct 2002 18:30:03 -0400
Subject: Re: JEOL 840A airlock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ritch,
The valve isn't that hard to get to. JEOL uses all those nice
easy-to-assemble connectors (can't say quick connect 'cause they're not
QF or KF) so after cooling the system down (although if you're really
careful, I think I did it with a hot system - look at it very carefully
before proceding hot), you can detatch a pair of solenoid valves from
the roughing manifold. DO NOT separate the valves from the pipe
fittings. JEOL epoxies them together and you may destroy something
trying to separate them. Just move the assembly out to where it is a
little easier to work on and disassemble the valve and remove the
plunger. I bought a new valve ($100.00 US)for one because I was only 20
miles from their US headquarters and just replaced the plunger, but for
the second one (which hasn't been installed yet) I brought the old
plunger home and dug out all the old, hard rubber. Then I took a cork
borer (I forget what size, but a snug fit) and cut a plug out of a
rubber stopper, cut it to the appropriate length, stuck some "Goop[" (an
everything, thick adhesive) into the hole in the plunger and rammed the
plug in so that is was level with the end of the plunger and let it set.
I'm expecting it to give me a good seal and a long life.


Ken Converse
owner
Quality Images
Delta, PA


Ritchie Sims wrote:

} Hi Ken
}
}
}
}
} } The second was not so bad, but also having to do with the airlock. If
} } the airlock is left open (not pumped) most of the time then the
} } airlock vent valve (a small solenoid valve) gets quite hot after a
} } while and remains hot (sometimes for years on end). This hardens the
} } rubber insert in the plunger so that it will no longer seal properly.
} } JEOL operates their airlock on a timer rather than from Pirrani
} } readings, so when it times out it says you can transfer the specimen.
} } This can lead to a considerable gas pulse and some minor
} } backstreaming from the DP before the high vacuum valve closes.
} }
} }
} }
}
} Yeah, my 840A has that condition. I put three manual ball-type valves
} between the airlock and a spare rotary pump, with a T/C vacuum
} gauge in there too, as it seemed like I was going to have to do a lot of
} dismantling to fix that valve. The manual system works so well, I find
} that 10 minutes sucking on samples before insertion really reduces the
} degradation of chamber vacuum on opening the gate valve, that I've
} left it like that.
}
} But maybe I should fix the vent valve anyway. Anybody got any tips on
} how that get at the vent valve with the minimum of collateral
} disassembly?
}
} cheers
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}
}
}
}





From daemon Wed Oct 9 20:48:41 2002



From: Allen Sampson :      ars-at-sem.com
Date: Wed, 9 Oct 2002 20:42:48 -0700
Subject: RE: JEOL 840A airlock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I haven't run across that problem myself, but sounds like a situation where
a simple circuit modification would be the best route.

In order to activate the valve, a small power transistor would be used to
ground one end of the coil while the other end is always attached to the
power supply. A small voltage (5V) would activate the transistor (ground
the coil and open the valve), 0V would deactivate it. Send that signal
instead to a simple multivibrator one-shot circuit set up to produce a
pulse with a duration of perhaps a minute, and send that to the transistor.
The airlock vents in just a few seconds, so it will always vent OK, but
would shut a minute later, thus limiting the time it is left activated.
Properly designed, if the excitation circuit goes low, the circuit would
automatically reset, in case the operator hits the button again and starts
the airlock roughing before the timer times out.

I don't have those schematics in front of me, but it's a pretty standard
way of running a solenoid valve. It should be easy to find someone to make
such a simple modification.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, October 09, 2002 1:01 PM, Ritchie Sims
[SMTP:r.sims-at-auckland.ac.nz] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Ken
}
}
} }
} } The second was not so bad, but also having to do with the airlock. If
} } the airlock is left open (not pumped) most of the time then the
} } airlock vent valve (a small solenoid valve) gets quite hot after a
} } while and remains hot (sometimes for years on end). This hardens the
} } rubber insert in the plunger so that it will no longer seal properly.
} } JEOL operates their airlock on a timer rather than from Pirrani
} } readings, so when it times out it says you can transfer the specimen.
} } This can lead to a considerable gas pulse and some minor
} } backstreaming from the DP before the high vacuum valve closes.
} }
}
} Yeah, my 840A has that condition. I put three manual ball-type valves
} between the airlock and a spare rotary pump, with a T/C vacuum
} gauge in there too, as it seemed like I was going to have to do a lot of
} dismantling to fix that valve. The manual system works so well, I find
} that 10 minutes sucking on samples before insertion really reduces the
} degradation of chamber vacuum on opening the gate valve, that I've
} left it like that.
}
} But maybe I should fix the vent valve anyway. Anybody got any tips on
} how that get at the vent valve with the minimum of collateral
} disassembly?
}
} cheers
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}
}



From daemon Wed Oct 9 21:55:15 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 09 Oct 2002 21:43:39 -0500
Subject: Re: View Petrographic Slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How about a projecting microscope? Built somewhat like an inverted on
a really tall stand, and no tube. The objective projects the image
onto a surface (like a sheet of paper) 30 - 50 cm or more below the
stage. Great for drawing specimens, and should work for this purpose,
although you'll need to darken the room.
Also cheap, and old tech, so there should be plenty of used units around.
Phil

} Group,
}
}
} Looking for a method of projecting a petrographic thin section
} (27X46mm) onto a large screen to be viewed by students. Have tried
} to sandwich the slide between two pieces of polarized film and
} display on a microfiche reader and that works pretty well but would
} like a larger brighter image.
}
} Have tried a digital camera on a petro microscope and again works
} well but area of view is limited.
}
} Any ideas?
}
} Roy Beavers
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, Tx. 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-mail.smu.edu
}

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Wed Oct 9 22:37:37 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 09 Oct 2002 23:36:53 -0400
Subject: Re: View Petrographic Slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Roy,

Get a plastic photo slide frame; trim away the ridges on one side that are intended to hold the 35mm film in place so that the petro slide can stick out without being shortened; cut a piece of polarizing film to fit inside the frame behind the petro slide and close it with a piece of tape.

Next put a piece of polarizing film at right angles to the first over the front of a projector lens; drop the slide in its mount into the projector with the polarizer behind, and away you go. If it is an autofocus projector you may need to disable the autofocus drive so as to prevent it from
focusing on the rear surface of the polarizer instead of the thin section.

This is also a means of photographing a very coarse grained rock between crossed polars when the field of view would exceed that of your microscope. Simply set up the camera over the projector and photograph the image on the screen. This works better than using a slide scanner and two pieces
of polarizing film, in many cases, because it keeps the polarizers out of the focal plane so that their imperfections are not superimposed on the thin section.

John Twilley
Conservation Scientist

Beavers, Roy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Group,
}
} Looking for a method of projecting a petrographic thin section (27X46mm) onto a large screen to be viewed by students. Have tried to sandwich the slide between two pieces of polarized film and display on a microfiche reader and that works pretty well but would like a larger brighter image.
}
} Have tried a digital camera on a petro microscope and again works well but area of view is limited.
}
} Any ideas?
}
} Roy Beavers
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, Tx. 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-mail.smu.edu





From daemon Thu Oct 10 00:48:02 2002



From: Arthur Day :      ard-at-ansto.gov.au
Date: Thu, 10 Oct 2002 15:37:51 +1000
Subject: Re: JEOL 840A airlock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Rtch,

When I did it on our 6400 (which in our case had exactly the same
valve as on the 840 we used to have), I actually bought a whole
replacement solenoid valve in preparation. However, as you've
implied, it is a bit of a mongrel of a task to chop the whole thing
out so in the end all I did was pull the solenoid plunger out of the
existing valve (with the little bit of heat-damaged rubber on its
end) and replaced it with just the new plunger and rubber bit from
the new valve. That way the whole task was a lot less invasive and
offensive to the instrument than it otherwise could have been....

So, see if you can get to just the plunger and then maybe you could
go one better and figure out a way to restore/replace the tiny little
bit of sealing rubber on its end.....

Cheers

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/


From daemon Thu Oct 10 01:54:11 2002



From: Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Thu, 10 Oct 2002 08:45:49 +0200
Subject: Re: View Petrographic Slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Roy,
Have you thought about acquiring one of these things called a "petroscope"? It's a bit like a glorified microfiche reader but designed for looking at thin-sections with lots of people about. It has a screen about the same size as a moderate TV, different magnifications and PPL and XPL
options. The company that produces these now has a digital camera attachment option available.
Just like to add that I have no interest in this company or whoever they are. And even though we have a petroscope in the department, it is rarely used for reasons that are uncertain.
Cheers,
Malc.

"Beavers, Roy" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Group,
}
} Looking for a method of projecting a petrographic thin section (27X46mm) onto a large screen to be viewed by students. Have tried to sandwich the slide between two pieces of polarized film and display on a microfiche reader and that works pretty well but would like a larger brighter image.
}
} Have tried a digital camera on a petro microscope and again works well but area of view is limited.
}
} Any ideas?
}
} Roy Beavers
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, Tx. 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-mail.smu.edu
}

--
Dr MP Roberts Phone: [+27](0)46 603 8313 (work)
Dept of Geology [+27] (0)46 6361197 (home)
Rhodes University Fax: [+27](0)46 622 9715
6140 Grahamstown Cell: 083 4060 262
SOUTH AFRICA e-mail: m.roberts-at-ru.ac.za




From daemon Thu Oct 10 02:22:27 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 10 Oct 2002 09:14:17 +0200
Subject: Re: View Petrographic Slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I remember Leitz had such a device, a slide projector modified to put
polariser, rotating analyser, and rotating mount (microscope like) for the
slide. We made a copy, modifying a old 2.1/4x2.1/4 SFOM slide projector.
We put the analyser on the objectif, to be able to rotate it. It's a bit
more difficult to make a rotary device fr the slide, but on such a
projector you have place enough to put a rotating table from an old
microscope. Our worked well. The 2.1/4x2.1/4 slide give you more space to
put the petrographic slide than the 24x36, and the objectives will give a
very good large image on the screen. You can than project it on a frosted
glass or a sheet of tracing paper to take a picture with a digital camera
and to save it as digital document.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Wed, 9 Oct 2002, Beavers, Roy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Group,
}
}
} Looking for a method of projecting a petrographic thin section (27X46mm) onto a large screen to be viewed by students. Have tried to sandwich the slide between two pieces of polarized film and display on a microfiche reader and that works pretty well but would like a larger brighter image.
}
} Have tried a digital camera on a petro microscope and again works well but area of view is limited.
}
} Any ideas?
}
} Roy Beavers
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, Tx. 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-mail.smu.edu
}
}



From daemon Thu Oct 10 05:28:35 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 10 Oct 2002 11:19:37 +0100 (GMT Daylight Time)
Subject: Re: ESEM for untreated bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the UK we have to make a safety assessment re using
unfixed bacteria in the ESEM. For instance I insist that
clinical urinary catheters are fixed.

Unfixed bacteria.
The magic of ESEM is that you can just have a go and then
look round for improvements! I always start with the
easiest method.

If they are in suspension I would pellet them gently and
rinse in distilled water and spin gently. Put the pellet
on the Peltier stage at 5 degree C with a little water to
cover it. Pump down at 7.5 Torr to avoid dehydration then
slowly reduce pressure to remove water and expose bacteria.

Disclaimer: ESEM images of bacteria have a different
quality to those that have been dried and gold coated; so
warn your user to avoid disappointment.

Let us know if this works for you!

Dave


On Wed, 09 Oct 2002 11:05:53 -0400 Ann-Fook Yang
{yanga-at-agr.gc.ca} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have been asked to provide a protocol for observing untreated bacteria in ESEM. Can anyone help?
}
}
} AnnFook Yang
} EM Unit,
} Eastern Cereal and Oilseed Research Centre,
} Room 2091, Bldg. 20,
} Central Experimental Farm,
} Ottawa, Ontario
} Canada K1A 0C6
}
} Tel: 1-613-759-1638
} Fax: 1-613-759-1701
}
} e-mail: yanga-at-em.agr.ca
}
} } } } Letizia Anello {anello-at-ibs.pa.cnr.it} 10/03/02 08:50AM } } }
} Dear Ann Fook,
}
} I am a molecular biologist and have no notion on
} electron microscopy and technical details; so I have collaboration with
} a chemical engineer of Palermo University. We have tried to examine
} unsuccessfully cultured bacteria directly under low vacuum SEM and ESEM
} (can you tell me the enlargement and pressure value to use?). I'd prefer
} observe in ESEM, completely untreated bacterial samples. Can you send me
} a detail protocol? Can I have any photo of yours of bacterial samples in
} SEM and ESEM? Thanks, bye.
}
} Letizia
}
} =====================================
}
}
}
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Oct 10 05:49:17 2002



From: mohammed y abdulrawoof / f40z006 75760 :      mdyousuf-at-KSU.EDU.SA
Date: Thu, 10 Oct 2002 13:35:19 -0300 (GMT)
Subject: en-block verses post-embedding staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
Please advise as to the usefulness/advantage of doing enblock staining of
biological tissues as against doing heavy-metal staining of grids with
thin sections. The tissues that I am about to process are for diagnosis
(pathology).

Thanks
M. Yousuf Abdul-Rawoof



From daemon Thu Oct 10 06:07:32 2002



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Thu, 10 Oct 2002 13:05:28 +0200
Subject: EU Spending on Nanotechnology double that of US?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

The European NanoBusiness Association recently produced a report on EU spending on nanotechnology - with the conclusions being that it is currently higher than the US, and very focused on applications and solutions. For anyone interested, it is a free download from www.nanoeurope.org

Best

Tim

*****************************************************************
Empowering business to make rational decisions about nanotechnology

CMP Cientifica
http://www.cmp-cientifica.com

Tim E. Harper - CEO
+34 91 640 71 85 (direct)
+ 34 91 640 71 86 (fax)
} From USA (877) 295-4480 (phone/fax)

CV: http://www.google.com/search?q=tim+%2B+harper+%2B+nanotechnology

The X Report - The crossroads of info- bio- & nano- tech
www.cmp-cientifica.com/x

The European Nanobusiness Association
www.nanoeurope.org

NanotechWeb - News and Information
www.nanotechweb.org

Nanoelectronics Reseach - The Phantoms Network
www.phantomsnet.com




From daemon Thu Oct 10 07:30:22 2002



From: John.Catino-at-mineralstech.com
Date: Thu, 10 Oct 2002 08:21:57 -0400
Subject: Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ANALYTICAL INVESTIGATOR


Minerals Technologies Inc., an international resource and technology based
organization, currently has an opening for a Analytical Investigator
Easton, PA facility

The Analytical Investigator is responsible for developing and completing
analytical investigations of samples, maintaining technical mastery and
awareness for microscopy techniques, assisting in the accreditation to ISO
17025, and completing administrative duties. The primary duty is
customer-oriented problem solving using optical and electron microscopy and
microchemical analysis in a team environment. FT-IR and GC-MS techniques
may also be utilized. The Analytical Investigator completes work of a
varied nature and is responsible for making and implementing most of the
decisions relevant to their area of responsibility.

Requirements include a Bachelor's degree in the sciences preferably
chemistry or mineralogy and a minimum of 5 years microscopy experience in a
service environment. Experience in computer assisted microscopy is highly
desirable. All candidates must possess good oral and written communication
skills, computer skills, ability to interact with a variety of people,
willingness to work in a team environment, to work extended hours when
necessary and ability to operate instrumentation and to manipulate
equipment and materials weighing 30-50 pounds. Previous experience with
LIMS, XRF or ICP spectroscopy is desirable.

MTI offers a competitive compensation and benefits package. Please submit
resume and cover letter with salary history to:

Bernadette Palumbo
Human Resources Development Manager
Minerals Technologies Inc.
640 N. 13th Street
Easton, PA 18042
Fax: 610-250-3210
Email: bpalumbo-at-mineralstech.com
No phone calls, please. Only those candidates meeting our requirements will
be contacted. Equal Opportunity Employer M/F/D/V.



John Catino
Minerals Technologies, Inc.
640 N. 13th Street
Easton, PA 18042
Phone: 610-250-3363
Fax: 610-250-3206
e-mail: john.catino-at-mineralstech.com



**********************************************************************
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are addressed. If you have received this email in error please notify
the system manager.

This footnote also confirms that this email message has been swept by
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From daemon Thu Oct 10 07:32:40 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Thu, 10 Oct 2002 08:26:18 -0400
Subject: Re: ESEM for untreated bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


First of all you MUST consider the health ramifications of untreated
pathogens in the chamber. They can contaminate the whole instrument and be
aerosolized into the room. I assume this has been considered and only
provide for the sake of completeness since this is going out to the whole
list.

Instrumentation wise as far as I know this can only be done with the
FEI/Philips/Electroscan instruments capable of condensing water in the
chamber. Not sure of the pressure ranges for the other manufacturers, but at
2 Torr chamber pressure you would freeze the sample before being able to
condense water or maintain the hydrated state. A field emission instrument
would give best results, but I have a LaB6 instrument and imaged similar
quite nicely on the Peltier stage cooled to 1C and 5 Torr vapor pressure.

Procedure wise there are several issues to overcome. First is the culture
itself. Is it a broth or plated. Both can be imaged each presenting
different issues. Precipitation of salts is an issue for both. For a plated
sample simply punch a thin core out and place flat on a petri. Trim excess
agar away. Surround with a drop of distilled water without letting any get
on top. This will remove some of the salts that could punch through the
colony. This step can be omitted if you are real careful with your vacuum
conditions. Plated samples may also have a polysaccharide film covering them
preventing imaging of the organism.

Broth cultures again have all kinds of problems with salts and gunk coating
the organism. They should be centrifuged loosely down, and washed with
distilled water. Place a drop of resuspended organism directly on the
Peltier stage and carefully evaporate the water in the chamber.

Both are still highly susceptible to the vacuum, reduced though it is, and
beam damage so image quickly and with lower voltages. This is really why a
FE-ESEM would be recommended.

Good luck

Scott Whittaker
Laboratories of Analytical Biology
Smithsonian Institution
National Museum of Natural History
PO Box 37012 MRC104
Washington DC 20013-7012
202-357-1651


} } } "Ann-Fook Yang" {yanga-at-agr.gc.ca} 10/09/02 11:05AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have been asked to provide a protocol for observing untreated bacteria in
ESEM. Can anyone help?


AnnFook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Room 2091, Bldg. 20,
Central Experimental Farm,
Ottawa, Ontario
Canada K1A 0C6

Tel: 1-613-759-1638
Fax: 1-613-759-1701

e-mail: yanga-at-em.agr.ca

} } } Letizia Anello {anello-at-ibs.pa.cnr.it} 10/03/02 08:50AM } } }
Dear Ann Fook,

I am a molecular biologist and have no notion on
electron microscopy and technical details; so I have collaboration with
a chemical engineer of Palermo University. We have tried to examine
unsuccessfully cultured bacteria directly under low vacuum SEM and ESEM
(can you tell me the enlargement and pressure value to use?). I'd prefer
observe in ESEM, completely untreated bacterial samples. Can you send me
a detail protocol? Can I have any photo of yours of bacterial samples in
SEM and ESEM? Thanks, bye.

Letizia

=====================================








From daemon Thu Oct 10 07:36:47 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Thu, 10 Oct 2002 05:30:24 -0700 (PDT)
Subject: Re: Fwd: CLAIMS/INVESTMENTS FROM HIS HIGHNESS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary, I simply forwarded the e-mail from HIS HIGHNESS
the Emir of Bahrain to Joe Udah from the Department of
Petroleum Resources in Nigeria. Let them work it out
amongst themselves!

Stu Smalinskas

__________________________________________________
Do you Yahoo!?
Faith Hill - Exclusive Performances, Videos & More
http://faith.yahoo.com


From daemon Thu Oct 10 10:18:20 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 10 Oct 2002 11:07:40 -0400
Subject: DPR

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Udah;

I will refer your request to a high powered government official in the
Federal Prosecutor's Office in Newark, New Jersey.

Sincerely,

P. Tomic

-----Original Message-----
} From: ENGR. JOE UDAH [mailto:joeudah123-at-dpr-nig.com]
Sent: Wednesday, October 09, 2002 11:49 AM
To: microscopy-at-sparc5.microscopy.com



DEPARTMENT OF PETROLEUM RESOURCES
PLOT 225 KOFO ABAYOMI STREET VICTORIA ISLAND,LAGOS, NIGERIA.
DIRECT TEL: 234 1 7591519 / FAX: 234 1 7590904
ATTENTION: THE PRESIDENT/C.E.O
RE: URGENT & CONFIDENTIAL BUSINESS PROPOSAL

Dear Sir,

I am ENGR. JOE UDAH ,member committee of the above department
Terms of Reference
My term of reference involves the award of contracts to multinational
companies.
My office is saddled with the responsibility of contract award, screening,
categorization and prioritization of projects embarked upon by Department of
Petroleum Resources (DPR) as well as feasibility studies for selected
projects and supervising the project consultants involved. A breakdown of
the fiscal expenditure by this office as at the end of last fiscal quarter
of 2000 indicates that DPR paid out a whooping sum of US$736M(Seven Hundred
And Thirty Six Million, United States Dollars) to successful contract
beneficiaries. The DPR is now compiling beneficiaries to be paid for the
third Quarter of 2002.
The crux of this letter is that the finance/contract department of the DPR
deliberately over -invoiced the contract value of the various contracts
awarded. In the course of disbursements, this department has been able to
accumulate the sum of US$38.2M(Thirty-eight Million, two hundred Thousand
U.S Dollars) as the over-invoiced sum. This money is currently in a suspense
account of the DPR account with the Debt Reconciliation Committee (DRC). We
now seek to process the transfer of this fund officially as contract payment
to you as a foreign contractor, who will be fronting for us as the
beneficiary of the fund. In this way we can facilitate these funds into your
nominated account for possible investment abroad. We are not allowed as a
matter of government policy to operate any foreign account to transfer this
fund into.
However, for your involvement in assisting us with this transfer into your
nominated account we have evolved a sharing formula as follows:
(1) 20% for you as the foreign partner
(2) 75% for I and my colleagues

(3) 5% will be set aside to defray all incidental expenses both Locally and
Internationally during the course of this transaction.

We shall be relying on your advice as regard investment of our share in any
business in your country. Be informed that this business is genuine and 100%
safe considering the high-power government officials involved. Send your
private fax/telephone numbers. Upon your response we shall provide you with
further information on the procedures. Feel free to send response by Fax or
TEL; expecting your response urgently. All enquiries should be directed to
the undersigned by FAX OR PHONE.
Looking forward to a good business relationship with you.

Sincerely,
ENGR. JOE UDAH.




From daemon Thu Oct 10 11:01:04 2002



From: saram-at-duke.edu
Date: Thu, 10 Oct 2002 11:47:20 -0400 (EDT)
Subject: Re: en-block verses post-embedding staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Uranyl acetate stabilizes lipids, membranes, and nucleic acids when used
as a fixative. Studies have shown less lipid extraction by the organic
solvents after UA fixation. It also contributes somewhat to contrast,
though not quite as much as osmium (i.e., if you leave out Os, and use UA
in fixation, there will be less contrast than if you use Os and not UA).
UA is generally used after osmication for additional stabilization and
contrast. It can be used in veranal acetate buffer, aqueous solutions,
or in alcoholic solutions.

We routinely use UV en bloc in pathology and virology samples EXCEPT in
cases where one needs to see glycogen deposits (e.g., glycogen storage
diseases). Thus, we never use UA in cases of heart, skeletal muscle, and
Ewing's sarcoma (if Ewing's is suspected).

UA and lead, used as post stains, on sections add a general contrast to
structures present (versus cytosol or plastic). In this case, UA does
not contribute to fixation or stabilization because the structures are
already embedded in resin.

Hope this helps.

Sara Miller


On Thu, 10 Oct 2002, mohammed y abdulrawoof / f40z006 75760 wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear listers,
} Please advise as to the usefulness/advantage of doing enblock staining of
} biological tissues as against doing heavy-metal staining of grids with
} thin sections. The tissues that I am about to process are for diagnosis
} (pathology).
}
} Thanks
} M. Yousuf Abdul-Rawoof
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Oct 10 11:53:04 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 10 Oct 2002 12:40:14 -0500
Subject: ESEM vs. gold coated SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

David Patton wrote:
==========================================================
Disclaimer: ESEM images of bacteria have a different quality to those that
} have been dried and gold coated; so warn your user to avoid
disappointment.
===========================================================

Are you comparing ESEM images with a) air dried and gold coated or b)
critical point dried and gold coated?

It would be obvious that comparing air dried would indeed show a difference
but if you really were comparing it to critical point dried samples, could
you elaborate a bit on the nature of those differences? And would you
have any comment about differences in contrast comparing ESEM vs. gold
coated images.


Disclaimer: SPI Supplies offers critical point dryers and sputter coaters so
I guess we would be a bit nervous of any technology that should evolve that
would render obsolete at the same time both CPD units and sputter coaters!

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================


From daemon Thu Oct 10 14:02:07 2002



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Thu, 10 Oct 2002 13:50:15 -0500
Subject: Thanks for Viewer ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,

Thanks for all of you who offered suggestions on how to project a large view of a petrographic slide.

With the help of my machine shop, I was able to design a petro slide holder that would fit into a standard 35mm slide projector. Once polarized film was taped to the outsides, we were able to project a large room size cross polar image of what students see in their microscopes at low mag.

Again thanks for the great ideas.

Roy Beavers
Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, Tx. 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-mail.smu.edu



From daemon Thu Oct 10 14:55:32 2002



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Thu, 10 Oct 2002 21:46:06 +0200
Subject: EU Spending on Nanotechnology double that of US?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

The European NanoBusiness Association recently produced a report on EU
spending on nanotechnology - with the conclusions being that it is
currently higher than the US, and very focused on applications and
solutions. For anyone interested, it is a free download from
www.nanoeurope.org

Best

Tim

*****************************************************************
Empowering business to make rational decisions about nanotechnology

CMP Cientifica
http://www.cmp-cientifica.com

Tim E. Harper - CEO
+34 91 640 71 85 (direct)
+ 34 91 640 71 86 (fax)
} From USA (877) 295-4480 (phone/fax)


The X Report - The crossroads of info- bio- & nano- tech

www.cmp-cientifica.com/x

The European Nanobusiness Association
www.nanoeurope.org

NanotechWeb - News and Information
www.nanotechweb.org

Nanoelectronics Reseach - The Phantoms Network
www.phantomsnet.com






From daemon Thu Oct 10 17:17:32 2002



From: John Shields :      jshields-at-cb.uga.edu
Date: Thu, 10 Oct 2002 18:05:39 -0400
Subject: Job opening - Avigenics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Post-doc/Microscopist

AviGenics, inc. (www.avigenics.com), the world leader in avian transgenesis
has an immediate post-doc or Research Associate position in our Technology
Development group. The appointment will be for one year, with possibility of
renewal.

The successful applicant will join a well funded, dynamic and
multidisciplinary team developing new transgenic technology combining
advanced microscopy methods, such as multiphoton, and cell and embryo
mechanical as well as laser-mediated micromanipulation techniques.

Hands-on experience with confocal and/or multiphoton microscopy is highly
desirable. We are particularly interested in candidates with demonstrated
experience on imaging optically opaque and live samples.

Requirements: preference will be given to candidates with a PhD degree, but
other suitable applicants with proven experience in the areas described
above will also be considered. Must be able to handle multiple projects
simultaneously, keeping accurate, detailed and well organized records, able
to work in a team environment and a have a strong interest in applied
research and technology development.

Salary and benefits including stock options will be commensurate with
experience

For information and submitting applications please contact:

Leandro Christmann DVM, MSc, PhD

Director of Technology Development

AviGenics, inc.

Georgia BioBusiness Center

111 Riverbend Rd.

Athens, GA 30605

Fax: (706) 227-2180

e-mail: christmann-at-avigenics.com




From daemon Thu Oct 10 17:52:53 2002



From: Libby Shaw :      elshaw-at-mit.edu
Date: Thu, 10 Oct 2002 19:10:20 -0400
Subject: Re: those REQUESTS FOR YOUR HELP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dorota,

Fight this with all you have.

The institution I just quit downgraded all of the lab staff positions. A
microbiologist with no em experience replaced me with 20 years of em
experience only because she lost her job due to downsizing and bumped me out
of my position. The management had recently changed (lowered) all of the
standards/qualifications so this event could take place. The union was
never informed of this move, but that is another story.

A supervisors position should not be part of the union. A smart manager
should realize that you cannot hire good help in this field into a
supervisor position without at least 7-10 years of EM experience.

The minimum education should be at least a BS in a biological field with at
least 5 years experience.

Cheers,

Ed Calomeni



----- Original Message -----
} From: "Dorota Wadowska" {wadowska-at-upei.ca}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, October 08, 2002 10:09 AM


You'll find an entertainingly relevant article at
http://www.theatlantic.com/issues/2002/10/working.htm

Libby Shaw




****************************************************************
Elisabeth L. Shaw, Facility Coordinator
Surface and Spectroscopy Labs
Analytical Shared Experimental Facilities
MIT Center for Materials Science and Engineering

Address: MIT Room 13-4149 Tel: 617-253-5045
77 Massachusetts Avenue Email: elshaw-at-mit.edu
Cambridge, MA 02139 Fax: 617-258-6478
http://web.mit.edu/cmse/www/
****************************************************************


From daemon Thu Oct 10 18:17:48 2002



From: sundeep.bhandari-at-nikon.co.uk (by way of Ask-A-Microscopist)
Date: Thu, 10 Oct 2002 18:11:05 -0500
Subject: Ask-A-Microscopist:epi-fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sundeep.bhandari-at-nikon.co.uk) from on Thursday,
October 10, 2002 at 12:16:30
---------------------------------------------------------------------------

Email: sundeep.bhandari-at-nikon.co.uk
Name: Sundeep Bhandari

Organization: Nikon UK

Education: Graduate College

Location: Kingston, Surrey, England

Question:
I am trying to find information about a technique i heard about a few
days ago, but can't find any information anywhere on the web. The
technique i am researching is 'polarised epi-fluorescence'. I
believe you can use it to determine the surface bound orientation of
fluorophores; i think the
excitation source is linearly polarised and one monitors the
fluorescence intensity as a function of polarisation angle.

Any information or pointers in the right direction would be greatly
appreciated.

kindest rgds
sundeep.

---------------------------------------------------------------------------


From daemon Thu Oct 10 18:17:49 2002



From: msandova-at-nd.edu (by way of Ask-A-Microscopist)
Date: Thu, 10 Oct 2002 18:11:59 -0500
Subject: Ask-A-Microscopist: Whipple Hauser optical piece

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (msandova-at-nd.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
October 10, 2002 at 17:40:13
---------------------------------------------------------------------------

Email: msandova-at-nd.edu
Name: Mayra

Organization: U of Notre Dame

Education: Graduate College

Location: Notre Dame, IN

Question: What is and how does one use a Whipple Hauser optical piece?
Thnx!

---------------------------------------------------------------------------


From daemon Thu Oct 10 18:21:29 2002



From: Marek Malecki, M.D., Ph.D., Professor :      mmalecki-at-facstaff.wisc.edu
Date: Thu, 10 Oct 2002 18:20:58 -0700
Subject: polishing 1/2 " Cambridge carbon SEM mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have attempted to polish /seal / ? carbon 1/2 " carbon SEM
mounts to obtain very smooth surface for HR SEM and be willing to share the
procedure? Ultrafine-grain sand paper , carbon colloid, and card-board did
not generate a surface which is smooth enough.


From daemon Thu Oct 10 20:47:32 2002



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Thu, 10 Oct 2002 21:38:50 -0400
Subject: FIB Focused Interest Group within MSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Microscopy Society of America has recently approved the formation of a
focused ion beam (FIB) focus interest group (FIG). The function of the FIB
FIG will be to organize a symposium for the Microscopy and Microanalysis
annual meeting, to hold an annual business meeting of the FIG, and at the
discretion of the members, hold a workshop or other FIB sponsored event
throughout the year. Individual membership is open to all MSA members at
an additional nominal cost of $10 per year above the annual MSA dues
commencing with the 2003 yearly MSA dues. A healthy membership within the
FIB FIG will ensure that FIB and related topics are an integral part of the
M&M meeting. In addition, members of the FIB FIG will have access to
workshops and programs designed to further the development and applications
of FIB. Vendors are welcome and encouraged to participate in the FIB FIG.
Vendors may join the FIB FIG at a nominal cost of $50 per year.

The first FIB FIG workshop is tentatively set for Tuesday March 18, 2003,
at the University of Central Florida in Orlando during the joint annual
meeting of the FL AVS and Florida Society for Microscopy.

Our goal is to get 50 people to join the FIB FIG within the first year. If
you are interested in becoming a member of the FIB FIG and/or interested in
attending the 1st FIB FIG Workshop in March, 2003 please contact either:

Lucille Giannuzzi, Chair, FIB FIG
lag-at-mail.ucf.edu

Joe Michael, Vice Chair, FIB FIG
jrmicha-at-sandia.gov

or

Barry Carter, Secretary/Treas, FIB FIG
carter-at-cems.umn.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Fri Oct 11 10:10:19 2002



From: Marek Malecki, M.D., Ph.D., Professor :      mmalecki-at-facstaff.wisc.edu
Date: Fri, 11 Oct 2002 10:03:19 -0700
Subject: Re: polishing 1/2 " Cambridge carbon SEM mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Th. Bill:
What was ~ temp. which you were using?
Is that stuff conductive or you have to re-coat it?
Marek.


At 07:37 AM 10/11/02 -0400, you wrote:
} Apiezon W - a high temp vacuum wax, can be melted on to the surface and
} it will be perfectly smooth.
}
} Bill Miller
}
}
}
} At 06:20 PM 10/10/2002 -0700, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Oct 11 10:10:20 2002



From: Marek Malecki, M.D., Ph.D., Professor :      mmalecki-at-facstaff.wisc.edu
Date: Fri, 11 Oct 2002 10:07:47 -0700
Subject: RE: polishing 1/2 " Cambridge carbon SEM mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Th. Wharton:
Please let me know after you try polishing it. We have all possible mills
and grinders here, but the pins , which we have, are really tough.
Marek.


At 07:22 AM 10/11/02 -0500, you wrote:

} Marek,
}
} Is this graphitic carbon? If so, you might look into vitreous carbon. This
} stuff is really hard, and shiny like a silicon wafer. I was looking for a
} really featureless, low Z mount, but not toxic (beryllium), and stumbled on
} this. I've just started using it so I haven't tried polishing it yet but I
} think it should be considerably easier to polish than graphitic carbon.
}
} Here's where I got it (sorry, not an EM supplier so you won't get standard
} mount geometries here).
}
} www.atomergic.com
}
} Regards,
} Wharton
}
} *************************************************************
} Wharton Sinkler, PhD
} UOP LLC
} 25 E. Algonquin Rd.
} Des Plaines, IL 6017-5017
} 847-391-3878
}
}
} } -----Original Message-----
} } From: Marek Malecki, M.D., Ph.D., Professor
} } [SMTP:mmalecki-at-facstaff.wisc.edu]
} } Sent: Thursday, October 10, 2002 8:21 PM
} } To: microscopy-at-sparc5.microscopy.com
} } Subject: polishing 1/2 " Cambridge carbon SEM mounts
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Does anyone have attempted to polish /seal / ? carbon 1/2 " carbon SEM
} } mounts to obtain very smooth surface for HR SEM and be willing to share
} } the
} } procedure? Ultrafine-grain sand paper , carbon colloid, and card-board did
} }
} } not generate a surface which is smooth enough.



From daemon Fri Oct 11 14:23:45 2002



From: Gang Ning :      gning-at-mcw.edu
Date: Fri, 11 Oct 2002 14:11:03 -0500
Subject: Re: en-block verses post-embedding staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I agree with Dr. Sara Miller's point that UA en block staining in some extent
helps in preservation of ultrastructure as well as enhancement of the
contrast. However, it also has disadvantages such as one has to deal with UA,
a biohazard reagent, and sometimes background from crystal precipitation,
especially when ethanolic UA is used. More importantly, it is time-consuming
because it takes hours for aqueous UA solution to penetrate a sizable tissue
block. Therefore, I would say it all depends your tissue and purpose of your
analysis.

In my lab, we routinely do en block with samples of blood, tissue culture,
virus, bacteria and other microorganisms, which are believed more vulnerable
to solvent, but not with solid tissue. We have a lot of samples from kidney
biopsy and we didn't find en block made any difference from staining thin
sections whereas taking your time to have a sufficient osmication is much
more helpful to your structure.

Greg Ning



mohammed y abdulrawoof / f40z006 75760 wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear listers,
} Please advise as to the usefulness/advantage of doing enblock staining of
} biological tissues as against doing heavy-metal staining of grids with
} thin sections. The tissues that I am about to process are for diagnosis
} (pathology).
}
} Thanks
} M. Yousuf Abdul-Rawoof

--
Gang Ning, M.D., Ph.D.
Director
Electron Microscopy Facility
Medical College of Wisconsin
8701 Watertown Plank Road
Milwaukee, Wisconsin 53226
(414) 456-8344
e-mail: gning-at-mcw.edu
http://www.mcw.edu/cellbio/emfacility.html




From daemon Fri Oct 11 15:24:45 2002



From: Ralph Common :      Ralph.Common-at-ht.msu.edu
Date: Fri, 11 Oct 2002 15:56:32 -0400
Subject: LM: Wild M400 Photomakroskop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have recently acquired a Wild M400 Photomakroskop equipped for Polaroid
photography only. I would like to set it up for 35mm photography. I have
obtained an appropriate camera and controller, but the necessary relay lens
assembly (Wild part # 376 734) is no longer available. So far, I have been
unable to find anybody willing to part with one. Has anybody out there had
any success improvising a 35mm photography setup on the M400 using other
than the Wild original equipment? Any advice would be appreciated.

Ralph Common
Michigan State University
Division of Human Pathology
ralph.common-at-ht.msu.edu



From daemon Fri Oct 11 16:15:28 2002



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Fri, 11 Oct 2002 20:57:27 +0100
Subject: RE: DPR

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Mr. Udah;
}
} I will refer your request to a high powered government official in the
} Federal Prosecutor's Office in Newark, New Jersey.
}
} Sincerely,
}
} P. Tomic
}
} -----Original Message-----
} } From: ENGR. JOE UDAH [mailto:joeudah123-at-dpr-nig.com]
} Sent: Wednesday, October 09, 2002 11:49 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: DPR
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

snips fascinating but irrelevant

} further information on the procedures. Feel free to send response by Fax or
} TEL; expecting your response urgently. All enquiries should be directed to
} the undersigned by FAX OR PHONE.
} Looking forward to a good business relationship with you.
}
} Sincerely,
} ENGR. JOE UDAH.

Too late - the Enron management have already got it all stitched up :-))
--
Larry Stoter


From daemon Fri Oct 11 16:36:02 2002



From: William Oxberry :      William.Oxberry-at-downstate.edu (by way of
Date: Fri, 11 Oct 2002 16:29:43 -0500
Subject: Leica CPC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Listers-

We are considering purchasing the latest cryoworkstation (Leica CPC)
to plunge freeze samples into liquid propane in preparation for
freeze substitution. On our quote there was an automatic filling
device listed as optional and we decided not to purchase it to save
money (over $6000).
Now we are told that it is not optional but required because this
unit is not able to be filled manually.

Do any of you use this equipment and know if it can be filled
manually or needs an automatic filling device?

thanks in advance

Bill Oxberry
Downstate Medical Center
7182704472


From daemon Fri Oct 11 16:45:16 2002



From: saram-at-duke.edu
Date: Fri, 11 Oct 2002 17:35:39 -0400 (EDT)
Subject: Re: en-block verses post-embedding staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


See CAPS interspersed below for distinction from the original.

Sara Miller

On Fri, 11 Oct 2002, Gang Ning wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi
}
} I agree with Dr. Sara Miller's point that UA en block staining in some extent
} helps in preservation of ultrastructure as well as enhancement of the
} contrast. However, it also has disadvantages such as one has to deal with UA,
} a biohazard reagent,

A BIOHAZARD IS A BIOLOGICAL AGENT THAT IS HAZARDOUS (INFECTIOUS). UA IS
RADIOACTIVE AND IS A HEAVY METAL. IT, LIKE GLUTARALDEHYDE, OSMIUM, LEAD,
RESINS, PROPYLENE OXIDE, ETC., SHOULD BE HANDLED WITH CARE. HANDLED
PROPERLY, IT IS NOT ANY MORE DANGEROUS THAN THE OTHER NASTY CHEMICALS
ELECTRON MICROSCOPISTS HAVE TO USE. IT IS NOT A BIOLOGICAL AGENT.

and sometimes background from crystal precipitation,
} especially when ethanolic UA is used.

WE NEVER SEE PRECIPITATION. PERHAPS TRY DILUTING THE STAIN OR WASHING
THOROUGHLY BEFORE USING IT WILL ELIMINATE THE PRECIPITATE. UA IS NOT
SOLUBLE IN PHOSPHATE OR CACODYLATE BUFFER; IF YOU DON'T WASH OUT THESE
BUFFERS BEFORE ADDING UA IN VERANAL ACETATE BUFFER, UA IN WATER, OR UA IN
ETHANOL OR METHANOL, YOU CAN GET PRECIPATE.

More importantly, it is time-consuming
} because it takes hours for aqueous UA solution to penetrate a sizable tissue
} block.

WE USE 1 MM TISSUE BLOCKS AND STAIN FOR AN HOUR 1% UA IN 0.11M VERONAL
ACETATE BUFFER. IF THE TISSUE OR CELL PELLET IS SMALLER, YOU CAN GET AWAY
WITH 30 MIN IN UA.

Therefore, I would say it all depends your tissue and purpose of your
} analysis.
}
I AGREE. IT ALL DEPENDS ON YOUR PURPOSE. IF YOU WANT BEAUTIFUL
ULTRASTRUCTURE AND YOU'RE NOT LOOKING SPECIFICALLY FOR GLYCOGEN, USE UA EN
BLOCK. IF YOU'RE IN A HURRY OR YOU CARE TO SEE ALL THE GLYGOGEN, DON'T
USE IT.

} In my lab, we routinely do en block with samples of blood, tissue culture,
} virus, bacteria and other microorganisms, which are believed more vulnerable
} to solvent, but not with solid tissue. We have a lot of samples from kidney
} biopsy and we didn't find en block made any difference from staining thin
} sections whereas taking your time to have a sufficient osmication is much
} more helpful to your structure.
}
} Greg Ning
}
}
}
} mohammed y abdulrawoof / f40z006 75760 wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Dear listers,
} } Please advise as to the usefulness/advantage of doing enblock staining of
} } biological tissues as against doing heavy-metal staining of grids with
} } thin sections. The tissues that I am about to process are for diagnosis
} } (pathology).
} }
} } Thanks
} } M. Yousuf Abdul-Rawoof
}
} --
} Gang Ning, M.D., Ph.D.
} Director
} Electron Microscopy Facility
} Medical College of Wisconsin
} 8701 Watertown Plank Road
} Milwaukee, Wisconsin 53226
} (414) 456-8344
} e-mail: gning-at-mcw.edu
} http://www.mcw.edu/cellbio/emfacility.html
}
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Oct 11 20:48:00 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 11 Oct 2002 15:27:51 -1000 (HST)
Subject: Re: polishing 1/2 " Cambridge carbon SEM mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Marek-

} Does anyone have attempted to polish /seal / ? carbon 1/2 " carbon SEM
} mounts to obtain very smooth surface for HR SEM and be willing to share the
} procedure? Ultrafine-grain sand paper , carbon colloid, and card-board did
} not generate a surface which is smooth enough.

We have a user who gets excellent results by gluing a small piece of
aluminum foil onto the stub and burnishing it before mounting her
micromolluscs. The background is amazingly smooth. I've not tried to
duplicate her technique, so I can't claim it will work all the time and
with all brands of foil. I have seen machine marks on some foils. I'm also
not sure if she uses regular thickness or heavy-duty. I will email her for
details when she returns from the field (Tahiti)!

Bug me if you don't hear from me.

Aloha,
Tina


****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Sat Oct 12 09:50:09 2002



From: Marek Malecki, M.D., Ph.D., Professor :      mmalecki-at-facstaff.wisc.edu
Date: Sat, 12 Oct 2002 09:45:51 -0700
Subject: Re: polishing 1/2 " Cambridge carbon SEM mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks Tina:
Looking forward to the details.
We were attempting to polish Al stubs, but they came out not flat enough.
Marek.

PS
How is your 912 working?



At 03:27 PM 10/11/02 -1000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Oct 12 11:03:56 2002



From: Marek Malecki, M.D., Ph.D., Professor :      mmalecki-at-facstaff.wisc.edu
Date: Sat, 12 Oct 2002 11:00:09 -0700
Subject: HR EDX elemental mapping programs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello EDX Experts;
Would you be willing to share your experience with various EDX mapping
programs - in particular deconvolving elemental distribution with
corrections for binding and emission energies in alloys or particles
embedded in a matrix (C)?
Marek.


From daemon Sat Oct 12 13:54:22 2002



From: ggm-at-servidor.unam.mx (by way of Ask-A-Microscopist)
Date: Sat, 12 Oct 2002 13:48:23 -0500
Subject: Ask-A-Microscopist:SEM process de bacteria Tiobacillus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ggm-at-servidor.unam.mx) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
October 11, 2002 at 11:39:52
---------------------------------------------------------------------------

Email: ggm-at-servidor.unam.mx
Name: GUILLERMINA GLEZ.

Organization: UNAM

Education: Graduate College

Location: MEXICO, D.F., MEXICO.

Question: do you now how to process de bacteria Tiobacillus sulfurans to SEM?

especifilly, how to concentrate, and conditions to concentrate.

thanks.

---------------------------------------------------------------------------


From daemon Sat Oct 12 13:54:26 2002



From: gbarclay-at-trinidad.net (by way of Ask-A-Microscopist)
Date: Sat, 12 Oct 2002 13:47:53 -0500
Subject: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (gbarclay-at-trinidad.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
October 11, 2002 at 23:40:22
---------------------------------------------------------------------------

Email: gbarclay-at-trinidad.net
Name: Greg Barclay

Organization: University of the West Indies

Education: Graduate College

Location: St. Augustine, Trinidad, West Indies

Question: Light microscope terminology: We all know what a
stereomicroscope is and what a compound microscope is, but are they
not technically both the same thing? Should not the distinction be
between the "simple microscope," basically a highly corrected doublet
or triplet lens, forming the Dowland type of microscope, and any kind
of microscope with a separate eyepiece and objective lens? If so,
then both the stereo and binocular (or monocular) microscopes are
compond microscopes. I have been referring to "stereomicroscopes and
binocular microscopes" in our Life Sciences Dept, and having some
arguments with those who refer "stereomicroscopes and compound
microscopes," over what is really what.

---------------------------------------------------------------------------


From daemon Sat Oct 12 16:09:14 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 12 Oct 2002 16:57:48 -0500
Subject: Smooth carbon surfaces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Marek Malecki wrote:
================================================================
Does anyone have attempted to polish /seal / ? carbon 1/2 " carbon SEM
mounts to obtain very smooth surface for HR SEM and be willing to share the
procedure? Ultrafine-grain sand paper , carbon colloid, and card-board did
not generate a surface which is smooth enough.
================================================================
Highly Ordered Pyrolytic Graphite (HOPG) which can be freshly cleaved like
mica, will cleave to an atomically smooth surface, mirror reflective. The
size of the atomically smooth areas depend on the grade of HOPG that is
procured. While the cost at first might seem high, remember one can get a
number of cleavings out of one HOPG starting "block". This is the substrate
often times used by those doing SPM work because of its ultra smooth surface
relative to other ways to make smooth nonpolar surfaces in carbon or
graphite.

In addition, it is of high purity and other wise behaves like any other form
of carbon in terms of its chemical resistance and stability.

More information about HOPG can be found on URL
http://www.2spi.com/catalog/new/hopgsub.shtml

Disclaimer: SPI Supplies is a leading supplier of HOPG to microscopy
laboratories worldwide so we have a vested interest in seeing more of it
used in this kind of application.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sat Oct 12 17:37:46 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Sat, 12 Oct 2002 18:25:17 -0400
Subject: Re: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
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It seems to me that there jas always been one other difference between
the two. Most truly binocular (stereo) microscopes do not have an
inverted image, whereas most "compound" microscopes have an inverted image.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (gbarclay-at-trinidad.net) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
} October 11, 2002 at 23:40:22
} ---------------------------------------------------------------------------
}
}
} Email: gbarclay-at-trinidad.net
} Name: Greg Barclay
}
} Organization: University of the West Indies
}
} Education: Graduate College
}
} Location: St. Augustine, Trinidad, West Indies
}
} Question: Light microscope terminology: We all know what a
} stereomicroscope is and what a compound microscope is, but are they
} not technically both the same thing? Should not the distinction be
} between the "simple microscope," basically a highly corrected doublet
} or triplet lens, forming the Dowland type of microscope, and any kind
} of microscope with a separate eyepiece and objective lens? If so, then
} both the stereo and binocular (or monocular) microscopes are compond
} microscopes. I have been referring to "stereomicroscopes and binocular
} microscopes" in our Life Sciences Dept, and having some arguments with
} those who refer "stereomicroscopes and compound microscopes," over
} what is really what.
}
} ---------------------------------------------------------------------------
}
}
}





From daemon Sun Oct 13 00:54:19 2002



From: hgccgfdcgf6252b45-at-libero.it
Date: Sat, 12 Oct 0102 12:11:15 +0800
Subject: Viag and other products 7468w-5

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Subject: Viag and other products 7468w-5

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From daemon Sun Oct 13 15:34:22 2002



From: saram-at-duke.edu
Date: Sun, 13 Oct 2002 16:15:09 -0400 (EDT)
Subject: Re: en-block verses post-embedding staining

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Again, see CAPS below.
(CAPS ARE USED TO DIFFERENTIATE COMMENTS, NOT TO CONVEY HOSTILITY.)


On Sun, 13 Oct 2002, gang ning wrote:

} Thanks for the clear definition of biohazard. But it is obvious that
} 1) UA is a nasty/hazard chemical. Everyone wants to avoid it if possible,

UA CERTAINLY CAN BE CALLED NASTY. IT DOES REQUIRE SPECIAL HANDLING
PROCEDURES, AS DO OTHER CHEMICALS THAT WE USE AND CAN BE HANDLED SAFELY
UNDER THE PROPER CONDITIONS. NOT EVERYONE WANTS TO AVOID IT. FOLKS MUST
MAKE THEIR OWN JUDGEMENT AS TO WHETHER THE EXTRA PROCESSING TIME AND CARE
OF HANDLING ANOTHER CHEMICAL IS WORTH IT TO THEM. NOT USING UA IS FINE.
HOWEVER, DO NOT MAKE A GENERALIZATION ABOUT *EVERYONE* JUST BECAUSE YOU
CHOOSE NOT TO USE IT.

} 2) it does take at least 1 hr + time for washing thoroughly (how long?)
} for a 1 mm3 block .... it is clearly time-consuming procedure

IT DOES REQUIRE EXTRA TIME (1-2 HOURS). ONE CAN STAIN A 1 mm3 BLOCK FOR
30-60 MIN AND WASH FOR 30-60 MIN.

unless your turn around time is not an issue and working overtime is not a
} matter with your hospital policy,

TIME IS VERY MUCH AN ISSUE FOR US IN THE HOSPITAL SETTING. OUR TURN
AROUND TIME IS *ROUTINELY* NEXT DAY FOR SAMPLES FIXED IN GLUT, OS, UA.
TISSUE COMES IN IN THE AM, IS PROCESSED THAT DAY, BAKED OVERNIGHT, CUT AND
VIEWED THE NEXT DAY, AND PATHOLOGISTS HAVE MICROGRAPHS *IN HAND* THAT
AFTERNOON--ALMOST ALWAYS. MY TECHS RARELY HAVE TO WORK OVERTIME, AND WHEN
THEY DO, IT'S USUALLY BECAUSE OF EXTRA CUTTING OF HAVING TO RETRIEVE
SPECIMENS OUT OF PARAFFIN BLOCKS OR OFF SLIDES, NOT BECAUSE THEY PUT
SOMETHING INTO UA.

YOU ARE CLEARLY VERY NEGATIVE TOWARD THE USE OF UA. FINE. DON'T USE IT.
BUT DON'T BE SARCASTIC ABOUT SPECIMEN TURN AROUND TIME AND
TECHNICIAN OVERTIME, BECAUSE IT JUST ISN'T AN ISSUE IF SPECIMENS AND
WORKLOADS ARE MANAGED PROPERLY.

} 3) en block staining has adversary effect on showing glycogen so is not
} suitable for muscle, heart, liver and so on; there is not significant
} meaning for kidney .. then what else left? LOTS:

BRAIN, CILIA, TUMORS, VIRUSES, MUSCLE DISEASES OTHER THAN GLYCOGEN STORAGE
DISEASES, RESEARCH SAMPLES, ETC.


It is overkill for solid tissue blocks, IT IS NOT OVERKILL WHEN THE
BEST PRESERVATION, RATHER THAN DECREASING PROCESSING TIME IS OF
IMPORTANCE.

and 4) ethanolic/methanolic UA of higher concentration penetrates tissue
} much faster than other aqueous UA solutions and gives fresher image and
} better contrast but sometimes leave precipitation around cell member no
} matter how one washes before staining.

AS I SAID BEFORE, IF THE CORRECT BUFFER IS USED SO THAT UA DOES NOT
PRECIPTATE WITH IT, THERE IS NO PRECIPITATION. PERHAPS YOUR *HIGHER
CONCENTRATION* IS A CAUSE OF DIFFICULTY IN WASHING. SINCE UA IS SOLUBLE
IN ALCOHOL, UNBOUND, UNPRECIPITATED UA IS EASILY WASHED OUT BY THE
DEHYDRATING AGENT(S). IF YOU HAVE PRECIPITATE, IT IS MOST LIKELY DUE TO
SOME REACTION WITH THE SOLUTION LEFT IN THE CELL BEFORE YOU ADD THE UA, OR
PERHAPS THE *HIGHER* CONCENTRATION YOU USE ISN'T WASHED OUT???

} For EM work, the simpler and better, as long as it works.

YES, THE KEY IS, *AS LONG AS IT WORKS* FOR THE PURPOSE YOU HAVE. IF THE
PURPOSE IS TO PRODUCE THE BEST ULTRASTRUCTURE, AND IF MEMBRANES AND OR
LIPIDS ARE PART OF THE QUESTION, USE OF UA IS GOOD.

Overkill is always a problem. YES, NO ONE WANTS TO SPEND MORE TIME THAN
IS NECESSARY OR USE CHEMICALS THAT CAN BE HAZARDOUS IF NOT HANDLED
PROPERLY, BUT UA IS NOT ALWAYS OVERKILL.

IT DEPENDS ON YOUR APPLICATION. EACH TO HIS OWN.
}
}
}
} saram-at-duke.edu {mailto:saram-at-duke.edu} wrote:
}
} } See CAPS interspersed below for distinction from the original.
} }
} } Sara Miller
} }
} } On Fri, 11 Oct 2002, Gang Ning wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi
} } }
} } } I agree with Dr. Sara Miller's point that UA en block staining in some extent
} } } helps in preservation of ultrastructure as well as enhancement of the
} } } contrast. However, it also has disadvantages such as one has to deal with UA,
} } } a biohazard reagent,
} } }
} }
} } A BIOHAZARD IS A BIOLOGICAL AGENT THAT IS HAZARDOUS (INFECTIOUS). UA IS
} } RADIOACTIVE AND IS A HEAVY METAL. IT, LIKE GLUTARALDEHYDE, OSMIUM, LEAD,
} } RESINS, PROPYLENE OXIDE, ETC., SHOULD BE HANDLED WITH CARE. HANDLED
} } PROPERLY, IT IS NOT ANY MORE DANGEROUS THAN THE OTHER NASTY CHEMICALS
} } ELECTRON MICROSCOPISTS HAVE TO USE. IT IS NOT A BIOLOGICAL AGENT.
} }
} } and sometimes background from crystal precipitation,
} }
} } } especially when ethanolic UA is used.
} } }
} }
} } WE NEVER SEE PRECIPITATION. PERHAPS TRY DILUTING THE STAIN OR WASHING
} } THOROUGHLY BEFORE USING IT WILL ELIMINATE THE PRECIPITATE. UA IS NOT
} } SOLUBLE IN PHOSPHATE OR CACODYLATE BUFFER; IF YOU DON'T WASH OUT THESE
} } BUFFERS BEFORE ADDING UA IN VERANAL ACETATE BUFFER, UA IN WATER, OR UA IN
} } ETHANOL OR METHANOL, YOU CAN GET PRECIPATE.
} }
} } More importantly, it is time-consuming
} }
} } } because it takes hours for aqueous UA solution to penetrate a sizable tissue
} } } block.
} } }
} }
} } WE USE 1 MM TISSUE BLOCKS AND STAIN FOR AN HOUR 1% UA IN 0.11M VERONAL
} } ACETATE BUFFER. IF THE TISSUE OR CELL PELLET IS SMALLER, YOU CAN GET AWAY
} } WITH 30 MIN IN UA.
} }
} } Therefore, I would say it all depends your tissue and purpose of your
} }
} } } analysis.
} } }
} } I AGREE. IT ALL DEPENDS ON YOUR PURPOSE. IF YOU WANT BEAUTIFUL
} } ULTRASTRUCTURE AND YOU'RE NOT LOOKING SPECIFICALLY FOR GLYCOGEN, USE UA EN
} } BLOCK. IF YOU'RE IN A HURRY OR YOU CARE TO SEE ALL THE GLYGOGEN, DON'T
} } USE IT.
} }
} } } In my lab, we routinely do en block with samples of blood, tissue culture,
} } } virus, bacteria and other microorganisms, which are believed more vulnerable
} } } to solvent, but not with solid tissue. We have a lot of samples from kidney
} } } biopsy and we didn't find en block made any difference from staining thin
} } } sections whereas taking your time to have a sufficient osmication is much
} } } more helpful to your structure.
} } }
} } } Greg Ning
} } }
} } }
} } }
} } } mohammed y abdulrawoof / f40z006 75760 wrote:
} } }
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } } Dear listers,
} } } } Please advise as to the usefulness/advantage of doing enblock staining of
} } } } biological tissues as against doing heavy-metal staining of grids with
} } } } thin sections. The tissues that I am about to process are for diagnosis
} } } } (pathology).
} } } }
} } } } Thanks
} } } } M. Yousuf Abdul-Rawoof
} } } }
} } } --
} } } Gang Ning, M.D., Ph.D.
} } } Director
} } } Electron Microscopy Facility
} } } Medical College of Wisconsin
} } } 8701 Watertown Plank Road
} } } Milwaukee, Wisconsin 53226
} } } (414) 456-8344
} } } e-mail: gning-at-mcw.edu {mailto:gning-at-mcw.edu}
} } } http://www.mcw.edu/cellbio/emfacility.html
} } }
} } }
} } }
} } }
} } }
} }
} } Sara E. Miller, Ph. D.
} } P. O. Box 3712
} } Duke University Medical Center
} } Durham, NC 27710
} } Ph: 919 684-3452
} } FAX: 919 684-3265
} }
} }
}
} --
}
} Gang Ning, M.D., Ph.D.
}
} Director
}
} Electron Microscopy Facility
}
} Medical College of Wisconsin
}
} 8701 Watertown Plank Road
}
} Milwaukee, WI 53226
}
} (414) 456-8141
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Sun Oct 13 17:47:31 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Mon, 14 Oct 2002 08:30:58 +1000
Subject: Re: polishing 1/2 " Cambridge carbon SEM mounts

Contents Retrieved from Microscopy Listserver Archives
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Another option is to glue a small round glass coverslip onto the stub with
carbon paste, then coat with gold, then attach specimens, re-coat.

} Hi, Marek-
}
} } Does anyone have attempted to polish /seal / ? carbon 1/2 " carbon SEM
} } mounts to obtain very smooth surface for HR SEM and be willing to share the
} } procedure? Ultrafine-grain sand paper , carbon colloid, and card-board did
} } not generate a surface which is smooth enough.
}
} We have a user who gets excellent results by gluing a small piece of
} aluminum foil onto the stub and burnishing it before mounting her
} micromolluscs. The background is amazingly smooth. I've not tried to
} duplicate her technique, so I can't claim it will work all the time and
} with all brands of foil. I have seen machine marks on some foils. I'm also
} not sure if she uses regular thickness or heavy-duty. I will email her for
} details when she returns from the field (Tahiti)!
}
} Bug me if you don't hear from me.
}
} Aloha,
} Tina
}
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************




From daemon Sun Oct 13 19:56:08 2002



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Mon, 14 Oct 2002 10:48:52 +1000
Subject: Re: polishing 1/2 " Cambridge carbon SEM mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a preference for mounting a coated 0.5" coverslip onto the stub
with colloidal dag. The cover slip is coated with poly-L-lysine either
before or after mounting on the stub. This holds the sample well and
gives a smooth black background after sputter coating.
Regards
JVN

Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, Marek-
}
}
} } Does anyone have attempted to polish /seal / ? carbon 1/2 " carbon SEM
} } mounts to obtain very smooth surface for HR SEM and be willing to share the
} } procedure? Ultrafine-grain sand paper , carbon colloid, and card-board did
} } not generate a surface which is smooth enough.
} }
}
} We have a user who gets excellent results by gluing a small piece of
} aluminum foil onto the stub and burnishing it before mounting her
} micromolluscs. The background is amazingly smooth. I've not tried to
} duplicate her technique, so I can't claim it will work all the time and
} with all brands of foil. I have seen machine marks on some foils. I'm also
} not sure if she uses regular thickness or heavy-duty. I will email her for
} details when she returns from the field (Tahiti)!
}
} Bug me if you don't hear from me.
}
} Aloha,
} Tina
}
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
}
}
}


--
John V Nailon
Executive Officer and Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld



From daemon Mon Oct 14 06:27:56 2002



From: daniel eberhard :      daniel.eberhard-at-uni-bielefeld.de
Date: Mon, 14 Oct 2002 13:16:55 +0200
Subject: smooth muscle anti actin

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I´m searching for a smooth muscle anti-Actin antibody (recognizing mouse
smooth muscle) which also works on formaldehyde fixed tissue sections.
Any hints or recommendations are welcome.

Thanks in advance
Daniel



From daemon Mon Oct 14 07:42:35 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 14 Oct 2002 08:41:01 -0400
Subject: Re: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I must jump in on this one. A real "stereo" microscope has two parallel
optical paths. That is, two objective lenses. No microscope with one
objective lens can be "stereo." Albeit there may be two eyepieces but
without two separate optical paths the images in both eyepieces must
necessarily be identical. It's like listening to stereo sound but if the
left and right channels are identical, hence no depth or direction to the
sound.

Inverted or non-inverted images do not define a compound microscope since
many are made with an "image erector" which is simply an additional lens to
reverse the image so you view the sample as it is with the unaided eye.
Most "micromanipulator" systems have image erectors on them for that
purpose. It helps one to not have to re-wire one's neurons to interpret
left as right when having to use hand manipulation under the microscope. I
have found that wiring and re-wiring neurons problematic.

Peter
Anadigics, Inc.

-----Original Message-----
} From: qualityimages [mailto:qualityimages-at-netrax.net]
Sent: Saturday, October 12, 2002 6:25 PM
To: by way of Ask-A-Microscopist; Microscopy


It seems to me that there jas always been one other difference between
the two. Most truly binocular (stereo) microscopes do not have an
inverted image, whereas most "compound" microscopes have an inverted image.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (gbarclay-at-trinidad.net) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
} October 11, 2002 at 23:40:22
}
---------------------------------------------------------------------------
}
}
} Email: gbarclay-at-trinidad.net
} Name: Greg Barclay
}
} Organization: University of the West Indies
}
} Education: Graduate College
}
} Location: St. Augustine, Trinidad, West Indies
}
} Question: Light microscope terminology: We all know what a
} stereomicroscope is and what a compound microscope is, but are they
} not technically both the same thing? Should not the distinction be
} between the "simple microscope," basically a highly corrected doublet
} or triplet lens, forming the Dowland type of microscope, and any kind
} of microscope with a separate eyepiece and objective lens? If so, then
} both the stereo and binocular (or monocular) microscopes are compond
} microscopes. I have been referring to "stereomicroscopes and binocular
} microscopes" in our Life Sciences Dept, and having some arguments with
} those who refer "stereomicroscopes and compound microscopes," over
} what is really what.
}
}
---------------------------------------------------------------------------
}
}
}





From daemon Mon Oct 14 08:08:48 2002



From: Barbara Foster :      bfoster-at-mme1.com (by way of MicroscopyListserver)
Date: Mon, 14 Oct 2002 08:03:55 -0500
Subject: Re: EM job classification

Contents Retrieved from Microscopy Listserver Archives
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}
} }
} } Hi,
} }
} } I usually don't weigh in on this type of thing but, as a past negotiator
} } for the teachers in my old school system and as an ardent advocate of the
} } importance of experience in microscopy, here is my two cents:
} } 1. As a negotiator - I worked very closely with both the Mass Teachers
} } Association (MTA) and the National Teachers Association. Ironically, our
} } "supervisors" (curriculum supervisors, principals and vice principals)
} } needed the protection of our association (MTA and NEA preferred not to be
} } called unions but they filled the role of protectors and negotiators), so,
} } to offer them that support, we opened membership to them but, for
} } negotiation purposes, separated them into their own negotiating
} group. The
} } position of lab director
} }
} } 2. As for your 20 years' experience: I earn my living training
} } microscopists. I head a group of consultants who specialize in
} } training. I am also a contributing editor to two key publications. Our
} } company is THE primary source of market research in the microscopy and
} } imaging industry. With well over 20 year's experience in the field as a
} } microscopist, a trainer, and now, a recognized industry watcher, I will
} } tell you the following:
} } The general rule of thumb is that it takes, on average, at least a year
} for
} } a microscopist to become proficient. Why?
} } a.Unlike any other analytical discipline, microscopy demands a high level
} } of eye-brain-hand coordination.
} } b. Unlike most other disciplines, we don't have one or two major pieces of
} } instrumentation to master; we have multiple instrument systems. We
} have to
} } know how to prepare our samples, which type of microscopy to use, how to
} } interpret the myriad types of images each type of microscopy can produce,
} } and, finally, how to integrate a complex electronic system including
} } cameras, computers, and software, to collect, archive, process, and
} measure
} } our images.
} } c. Unlike most other disciplines, there is no replacement for the mental
} } library of images which a microscopist collects with experience. Yes, a
} } spectroscopist will learn the nuances of an upfield or downfield shift
} } characteristic of a specific spectrum, but no other discipline requires
} the
} } mental collection and correlation of image information like microscopy.
} }
} } As a master teacher and contributing editor, I know that I must weigh my
} } words carefully. However, as you can tell, I feel very strongly about the
} } erosion of microscopy experience which inevitably accompanies budget
} } cuts. Please feel free to quote me to your management on any or all of
} } these comments.
} }
} } Best regards... and strong support....
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
} }
} }
} }
} } At 06:43 PM 10/10/02 -0400, Ed Calomeni wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi Dorota,
} } }
} } } Fight this with all you have.
} } }
} } } The institution I just quit downgraded all of the lab staff positions. A
} } } microbiologist with no em experience replaced me with 20 years of em
} } } experience only because she lost her job due to downsizing and bumped
} me out
} } } of my position. The management had recently changed (lowered) all of the
} } } standards/qualifications so this event could take place. The union was
} } } never informed of this move, but that is another story.
} } }
} } } A supervisors position should not be part of the union. A smart manager
} } } should realize that you cannot hire good help in this field into a
} } } supervisor position without at least 7-10 years of EM experience.
} } }
} } } The minimum education should be at least a BS in a biological field
} with at
} } } least 5 years experience.
} } }
} } } Cheers,
} } }
} } } Ed Calomeni
} } }
} } }
} } }
} } } ----- Original Message -----
} } } } From: "Dorota Wadowska" {wadowska-at-upei.ca}
} } } To: {microscopy-at-sparc5.microscopy.com}
} } } Sent: Tuesday, October 08, 2002 10:09 AM
} } } Subject: EM job classificcation
} } }
} } }
} } } }
} ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi Listservers!
} } } } I have a few questions that I would like to ask you If you feel you
} } } } know the answer please reply. My university together with local
} } } } union reclassified staff positions according to Aiken plan. Needless
} } } } to say my position was downgraded.
} } } } My questions are:
} } } } 1. Can EM supervisory position be compared to any on campus
} } } } staff positions (university has Department of Sciences and
} } } } Veterinary Medecine)? I supervise small multi--user lab, we have 2
} } } } TEM, and we do research, diagnostics, teaching (under- and
} } } } graduate level) in the field of biological sciences. No fancy staff
} like
} } } } X-ray analysis or cryo.
} } } } 2. What in your opinion is a necessary minimum experience
} } } } required to perform this job ? I am talking about accepting the
} } } } position and immediately getting into the stream of work.
} } } } 3. What in your opinion is the minimum level of education that is
} } } } required to perform this job?
} } } }
} } } } You can reply directly to me. Thanks in advance
} } } } Frustrated
} } } } Dorota
} } } }
} }

{/x-flowed}


From daemon Mon Oct 14 10:12:03 2002



From: John W. Raffensperger, Jr. :      johnr-at-helwigcp.com
Date: Mon, 14 Oct 2002 09:58:57 -0500
Subject: RE: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just because a microscope has two eyepieces, does not mean that it is a
"stereo" microscope.

A stereo microscope has not only two eyepieces, but also two objectives,
and two separate light paths. Because of having two slightly offset
images, you see a three dimensional image. Usually these are lower
powered systems, but still compound microscopes (multiple lens systems).

On a high power (relatively) microscope with a binocular head, you only
have one objective, and one light path from the specimen to the head.
The prism(s) in the head divide the light path to provide multiple
images, but they are all (three in the case of an extra camera port)
identical. This does not provide a 3-D image, it just makes it easier
on the eyes and brain to process the image because one eye is not
distracted.

I am not a scientist, and would be interested in any corrections to the
above.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.


Email: gbarclay-at-trinidad.net
Name: Greg Barclay

Organization: University of the West Indies

Education: Graduate College

Location: St. Augustine, Trinidad, West Indies

Question: Light microscope terminology: We all know what a
stereomicroscope is and what a compound microscope is, but are they
not technically both the same thing?



From daemon Mon Oct 14 11:31:52 2002



From: Isabel Nogueira :      isabeln-at-popsrv.ist.utl.pt
Date: Mon, 14 Oct 2002 17:16:01 +0100
Subject: Glue for TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I need to prepare TEM cross-section samples of alternate SiO2 and TiO2
layers on a Si substrate. For that purpose I would appreciate suggestions on
glues, suitable for TEM work, that have a sputtering rate not too different
from my non homogeneous layers. The final sputtering will be on an Ion Mill
(4keV) using Ar+ ions.
All suggestions are welcome (including vendors) either to the list or to me
directly.
Thank you,

Isabel



Isabel Nogueira
Instituto Superior Técnico
Dep. Materiais
Avenida Rovisco Pais
1049-001 Lisboa
Portugal
tel.: +351 218418123
fax: +351 218418120
email: isabeln-at-popsrv.ist.utl.pt



From daemon Mon Oct 14 13:25:35 2002



From: Barbara Foster :      bfoster-at-mme1.com
Date: Mon, 14 Oct 2002 14:23:57 -0700
Subject: Fwd: Re: EM job classification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} From: Barbara Foster {bfoster-at-mme1.com}
} Subject: Re: EM job classification
}
} }
} } }
} } } Hi,
} } }
} } } I usually don't weigh in on this type of thing but, as a past negotiator
} } } for the teachers in my old school system and as an ardent advocate of the
} } } importance of experience in microscopy, here is my two cents:
} } } 1. As a negotiator - I worked very closely with both the Mass Teachers
} } } Association (MTA) and the National Teachers Association. Ironically, our
} } } "supervisors" (curriculum supervisors, principals and vice principals)
} } } needed the protection of our association (MTA and NEA preferred not to be
} } } called unions but they filled the role of protectors and negotiators),
} } so,
} } } to offer them that support, we opened membership to them but, for
} } } negotiation purposes, separated them into their own negotiating
} } group. The
} } } position of lab director
} } }
} } } 2. As for your 20 years' experience: I earn my living training
} } } microscopists. I head a group of consultants who specialize in
} } } training. I am also a contributing editor to two key publications. Our
} } } company is THE primary source of market research in the microscopy and
} } } imaging industry. With well over 20 year's experience in the field as a
} } } microscopist, a trainer, and now, a recognized industry watcher, I will
} } } tell you the following:
} } } The general rule of thumb is that it takes, on average, at least a
} } year for
} } } a microscopist to become proficient. Why?
} } } a.Unlike any other analytical discipline, microscopy demands a high level
} } } of eye-brain-hand coordination.
} } } b. Unlike most other disciplines, we don't have one or two major
} } pieces of
} } } instrumentation to master; we have multiple instrument systems. We
} } have to
} } } know how to prepare our samples, which type of microscopy to use, how to
} } } interpret the myriad types of images each type of microscopy can produce,
} } } and, finally, how to integrate a complex electronic system including
} } } cameras, computers, and software, to collect, archive, process, and
} } measure
} } } our images.
} } } c. Unlike most other disciplines, there is no replacement for the mental
} } } library of images which a microscopist collects with experience. Yes, a
} } } spectroscopist will learn the nuances of an upfield or downfield shift
} } } characteristic of a specific spectrum, but no other discipline
} } requires the
} } } mental collection and correlation of image information like microscopy.
} } }
} } } As a master teacher and contributing editor, I know that I must weigh my
} } } words carefully. However, as you can tell, I feel very strongly about
} } the
} } } erosion of microscopy experience which inevitably accompanies budget
} } } cuts. Please feel free to quote me to your management on any or all of
} } } these comments.
} } }
} } } Best regards... and strong support....
} } } Barbara Foster
} } } Microscopy/Microscopy Education
} } } 125 Paridon Street, Suite 102
} } } Springfield, MA 01118
} } } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} } }
} } }
} } }
} } }
} } } At 06:43 PM 10/10/02 -0400, Ed Calomeni wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi Dorota,
} } } }
} } } } Fight this with all you have.
} } } }
} } } } The institution I just quit downgraded all of the lab staff positions. A
} } } } microbiologist with no em experience replaced me with 20 years of em
} } } } experience only because she lost her job due to downsizing and bumped
} } me out
} } } } of my position. The management had recently changed (lowered) all of the
} } } } standards/qualifications so this event could take place. The union was
} } } } never informed of this move, but that is another story.
} } } }
} } } } A supervisors position should not be part of the union. A smart manager
} } } } should realize that you cannot hire good help in this field into a
} } } } supervisor position without at least 7-10 years of EM experience.
} } } }
} } } } The minimum education should be at least a BS in a biological field
} } with at
} } } } least 5 years experience.
} } } }
} } } } Cheers,
} } } }
} } } } Ed Calomeni
} } } }
} } } }
} } } }
} } } } ----- Original Message -----
} } } } } From: "Dorota Wadowska" {wadowska-at-upei.ca}
} } } } To: {microscopy-at-sparc5.microscopy.com}
} } } } Sent: Tuesday, October 08, 2002 10:09 AM
} } } } Subject: EM job classificcation
} } } }
} } } }
} } } } }
} } ------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} } -----------------------------------------------------------------------.
} } } } }
} } } } }
} } } } } Hi Listservers!
} } } } } I have a few questions that I would like to ask you If you feel you
} } } } } know the answer please reply. My university together with local
} } } } } union reclassified staff positions according to Aiken plan. Needless
} } } } } to say my position was downgraded.
} } } } } My questions are:
} } } } } 1. Can EM supervisory position be compared to any on campus
} } } } } staff positions (university has Department of Sciences and
} } } } } Veterinary Medecine)? I supervise small multi--user lab, we have 2
} } } } } TEM, and we do research, diagnostics, teaching (under- and
} } } } } graduate level) in the field of biological sciences. No fancy
} } staff like
} } } } } X-ray analysis or cryo.
} } } } } 2. What in your opinion is a necessary minimum experience
} } } } } required to perform this job ? I am talking about accepting the
} } } } } position and immediately getting into the stream of work.
} } } } } 3. What in your opinion is the minimum level of education that is
} } } } } required to perform this job?
} } } } }
} } } } } You can reply directly to me. Thanks in advance
} } } } } Frustrated
} } } } } Dorota
} } } } }
} } }



From daemon Mon Oct 14 16:05:36 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 14 Oct 2002 14:35:58 -0600
Subject: RE: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think, Peter and you are right with the statement that different light
paths are necessary to achive a true stereo effect. I am not so sure, that 2
separate objectives are really required. I know of at least one company (I
have no affiliation with or interest in that company) that makes a 3D
microscope and I think they are using only one objective lens
(http://www.edge-3d.com).

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: John W. Raffensperger, Jr. [mailto:johnr-at-helwigcp.com]
Sent: Monday, October 14, 2002 8:59 AM
To: 'by way of Ask-A-Microscopist'; Microscopy-at-sparc5.microscopy.com


Just because a microscope has two eyepieces, does not mean that it is a
"stereo" microscope.

A stereo microscope has not only two eyepieces, but also two objectives,
and two separate light paths. Because of having two slightly offset
images, you see a three dimensional image. Usually these are lower
powered systems, but still compound microscopes (multiple lens systems).

On a high power (relatively) microscope with a binocular head, you only
have one objective, and one light path from the specimen to the head.
The prism(s) in the head divide the light path to provide multiple
images, but they are all (three in the case of an extra camera port)
identical. This does not provide a 3-D image, it just makes it easier
on the eyes and brain to process the image because one eye is not
distracted.

I am not a scientist, and would be interested in any corrections to the
above.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.


Email: gbarclay-at-trinidad.net
Name: Greg Barclay

Organization: University of the West Indies

Education: Graduate College

Location: St. Augustine, Trinidad, West Indies

Question: Light microscope terminology: We all know what a
stereomicroscope is and what a compound microscope is, but are they
not technically both the same thing?



From daemon Mon Oct 14 16:34:17 2002



From: Anna Johansson :      anna.johansson-at-bioseeker.com
Date: Mon, 14 Oct 2002 16:28:27 -0500 (CDT)
Subject: BSG BioNewsletter *Antifungals*Swedish Biopharma* 05#2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


BSG BioNewsLetter 05#2002 - BioSeeker Group's analysis and products eNewsletter

URL: http://www.bioseeker.com http://www.bsg-online.biz

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4) Swedish Biotech Packages
5) Seek And You Will Find
6) Coming reports: HCV Therapy / Winning the Osteoporosis Therapy Race
7) Subscription
8) BioSeeker Online Store

====================================================================

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Treatment of HCV (Hepatitis C Virus) is a market with unmet medical needs, with relatively young people infected and a high risk for chronic disease. The coming decade will see exciting developments in HCV therapy thanks to breakthroughs in overcoming a major barrier to development: the lack of dedicated models suitable for validating candidate compounds in a low-cost, highthroughput format. BioSeeker Group has identified at least 115 companies with interest in various stages in the field of HCV therapy. Out of these 100, approximately 10 of the 'big pharma' are represented. Our coming report "Molecular Approaches Towards New HCV Therapy" analyses the field of new HCV therapeutics and assess possible outcomes for drugs and companies in this sector.

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From daemon Mon Oct 14 17:14:49 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 14 Oct 2002 18:01:56 -0400
Subject: Glue for TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Isabel,
There are two epoxy glues that work well. Epo-Tek 353ND from Epoxy Technology is one. Gatan's G1 is equivalent to this. The other is M-bond 610. I like the Epo-Tek 353ND; mostly because it has a longer shelf life, but also because I believe that it is stronger. I believe that you can get thinner glue joints with the M-bond 610, though.

BTW, Your system is ideal for using the small angle cleavage technique. You can forgo glueing, dimpling, and ion milling altogether and make about 10 samples per hour. Check out the South Bay Technology web site and look at the Microcleave kit and examples that they have. A detailed description of the technique has been written up by John McCaffrey and myself in the number 4 proceedings book on TEM sample preparation from the MRS. John also has the original publication in the number 3 book from MRS.
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."



-----Original Message-----
} From: Isabel Nogueira [mailto:isabeln-at-popsrv.ist.utl.pt]
Sent: Monday, October 14, 2002 12:16 PM
To: Microscopy-at-sparc5.microscopy.com


Hello,

I need to prepare TEM cross-section samples of alternate SiO2 and TiO2
layers on a Si substrate. For that purpose I would appreciate suggestions on
glues, suitable for TEM work, that have a sputtering rate not too different
from my non homogeneous layers. The final sputtering will be on an Ion Mill
(4keV) using Ar+ ions.
All suggestions are welcome (including vendors) either to the list or to me
directly.
Thank you,

Isabel



Isabel Nogueira
Instituto Superior Técnico
Dep. Materiais
Avenida Rovisco Pais
1049-001 Lisboa
Portugal
tel.: +351 218418123
fax: +351 218418120
email: isabeln-at-popsrv.ist.utl.pt



From daemon Mon Oct 14 17:43:21 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 14 Oct 2002 18:32:53 -0500
Subject: TEM cross-section glues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Isabel Nogueira wrote:
=========================================================
I need to prepare TEM cross-section samples of alternate SiO2 and TiO2
layers on a Si substrate. For that purpose I would appreciate suggestions on
glues, suitable for TEM work, that have a sputtering rate not too different
from my non homogeneous layers. The final sputtering will be on an Ion Mill
(4keV) using Ar+ ions.
All suggestions are welcome (including vendors) either to the list or to me
directly.
===========================================================
Several different glues have been mentioned on the listserver, one being M-
Bond™ 610, which is described on URL
http://www.2spi.com/catalog/spec_prep/glue.shtml

Disclaimer: SPI Supplies is a worldwide distributor for the M-Bond 610
product.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Mon Oct 14 19:56:04 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Mon, 14 Oct 2002 17:34:29 -0700
Subject: Re: Glue for TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Isabel:

Some very popular "glues" used for bonding TEM samples for cross section
analysis are:

Product Name: Epo-Tek 353ND

Epoxy Technology
TEL: 978-667-3805
FAX: 978-663-9782
www.epotek.com

Product Name: Loctite 460
http://www.loctite-europe.com/es/ (Website in Spain)

Loctite Corp
1001 Trout Brook Crossing
Rocky Hill, CT 06067 USA
TEL: 860.571.510
www.loctite.com

Product Name: M-Bond 610

Vishay Measurements Group, Inc.
P.O. Box 27777
Raleigh, North Carolina 27611 USA

TEL: 919-365-3800
FAX: 919-365-3945
measurementsgroup-at-vishay.com

I hope this helps.

Best regards-

David

--
David Henriks
Vice President

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 38 years of providing Materials Processing Solutions for
Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.
Isabel Nogueira wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
}
} I need to prepare TEM cross-section samples of alternate SiO2 and TiO2
} layers on a Si substrate. For that purpose I would appreciate suggestions on
} glues, suitable for TEM work, that have a sputtering rate not too different
} from my non homogeneous layers. The final sputtering will be on an Ion Mill
} (4keV) using Ar+ ions.
} All suggestions are welcome (including vendors) either to the list or to me
} directly.
} Thank you,
}
} Isabel
}
} Isabel Nogueira
} Instituto Superior Técnico
} Dep. Materiais
} Avenida Rovisco Pais
} 1049-001 Lisboa
} Portugal
} tel.: +351 218418123
} fax: +351 218418120
} email: isabeln-at-popsrv.ist.utl.pt



The information contained in this message and any attachments is
privileged and
confidential. This message is intended for the individual or entity
addressed.
If you are not the intended recipient, please do not read, copy or
disclose this
communication. Notify the sender of the mistake by calling
+1-949-492-2600 and
delete this message from your system.


From daemon Mon Oct 14 22:26:34 2002



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 14 Oct 2002 22:17:02 -0500
Subject: Re: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Almost all modern stereo microscopes use a single objective lens that have
two light paths of varying complexity viewing the specimen at different view
points through a single objective.

The light paths are not parallel but view the subject from a slightly
different angle and present them to the eye at an angle compatible with the
brains processing methods to make it appear as a 3D image. How the light
paths are handled between the subject and the eye may have them at any
relation to each other but when they reach the eye they are at an angle that
make them appear to be 3D images.

Viewing a subject at an angle though a common objective introduces some
distortion but less than viewing the same subject through two completely
separate microscopes that are an angle to the subject.

If you have doubts about this find a AO Cycloptic with misaligned lenses in
the cylinder that change the magnification and see some of the weird ways an
image can behave when it is presented to the eyes incorrectly.

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger


} From: "Peter Tomic" {PTomic-at-anadigics.com}
:
: I must jump in on this one. A real "stereo" microscope has two parallel
: optical paths. That is, two objective lenses. No microscope with one
: objective lens can be "stereo." Albeit there may be two eyepieces but
: without two separate optical paths the images in both eyepieces must
: necessarily be identical. It's like listening to stereo sound but if the
: left and right channels are identical, hence no depth or direction to the
: sound.
:
: Inverted or non-inverted images do not define a compound microscope since
: many are made with an "image erector" which is simply an additional lens
to
: reverse the image so you view the sample as it is with the unaided eye.
: Most "micromanipulator" systems have image erectors on them for that
: purpose. It helps one to not have to re-wire one's neurons to interpret
: left as right when having to use hand manipulation under the microscope.
I
: have found that wiring and re-wiring neurons problematic.
:
: Peter
: Anadigics, Inc.
:
: -----Original Message-----
: } From: qualityimages [mailto:qualityimages-at-netrax.net]
: Sent: Saturday, October 12, 2002 6:25 PM
: To: by way of Ask-A-Microscopist; Microscopy
: Subject: Re: Ask-A-Microscopist:LM terminology
:
:
: ------------------------------------------------------------------------
: The Microscopy ListServer -- Sponsor: The Microscopy Society of America
: To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
: On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
: -----------------------------------------------------------------------.
:
:
: It seems to me that there jas always been one other difference between
: the two. Most truly binocular (stereo) microscopes do not have an
: inverted image, whereas most "compound" microscopes have an inverted
image.
:
: Ken Converse
: owner
: Quality Images
: third party SEM service
: Delta, PA
:
: by way of Ask-A-Microscopist wrote:
:
: } ------------------------------------------------------------------------
: } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
: } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
: } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
: } -----------------------------------------------------------------------.
: }
: }
: } Below is the result of your feedback form (NJZFM-ultra-55). It was
: } submitted by (gbarclay-at-trinidad.net) from
: } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
: } October 11, 2002 at 23:40:22
: }
: --------------------------------------------------------------------------
-
: }
: }
: } Email: gbarclay-at-trinidad.net
: } Name: Greg Barclay
: }
: } Organization: University of the West Indies
: }
: } Education: Graduate College
: }
: } Location: St. Augustine, Trinidad, West Indies
: }
: } Question: Light microscope terminology: We all know what a
: } stereomicroscope is and what a compound microscope is, but are they
: } not technically both the same thing? Should not the distinction be
: } between the "simple microscope," basically a highly corrected doublet
: } or triplet lens, forming the Dowland type of microscope, and any kind
: } of microscope with a separate eyepiece and objective lens? If so, then
: } both the stereo and binocular (or monocular) microscopes are compond
: } microscopes. I have been referring to "stereomicroscopes and binocular
: } microscopes" in our Life Sciences Dept, and having some arguments with
: } those who refer "stereomicroscopes and compound microscopes," over
: } what is really what.
: }
: }
: --------------------------------------------------------------------------
-
: }
: }
: }
:
:
:
:
:
:



From daemon Tue Oct 15 07:15:36 2002



From: Jim Quinn :      jquinn-at-www.matscieng.sunysb.edu
Date: Tue, 15 Oct 2002 08:04:24 -0400
Subject: RE: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike -

re: stereo/terminology

I agree, my Lynx is stereo with only one objective.

http://www.visioneng.com/

Jim

} From Microscopy-request-at-sparc5.microscopy.com Tue Oct 15 03:54:02 2002
} From: Mike Bode {mb-at-Soft-Imaging.com}
} To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
} Subject: RE: Ask-A-Microscopist:LM terminology
} Date: Mon, 14 Oct 2002 14:35:58 -0600
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I think, Peter and you are right with the statement that different light
} paths are necessary to achive a true stereo effect. I am not so sure, that 2
} separate objectives are really required. I know of at least one company (I
} have no affiliation with or interest in that company) that makes a 3D
} microscope and I think they are using only one objective lens
} (http://www.edge-3d.com).
}
} mike
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: John W. Raffensperger, Jr. [mailto:johnr-at-helwigcp.com]
} Sent: Monday, October 14, 2002 8:59 AM
} To: 'by way of Ask-A-Microscopist'; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Ask-A-Microscopist:LM terminology
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Just because a microscope has two eyepieces, does not mean that it is a
} "stereo" microscope.
}
} A stereo microscope has not only two eyepieces, but also two objectives,
} and two separate light paths. Because of having two slightly offset
} images, you see a three dimensional image. Usually these are lower
} powered systems, but still compound microscopes (multiple lens systems).
}
} On a high power (relatively) microscope with a binocular head, you only
} have one objective, and one light path from the specimen to the head.
} The prism(s) in the head divide the light path to provide multiple
} images, but they are all (three in the case of an extra camera port)
} identical. This does not provide a 3-D image, it just makes it easier
} on the eyes and brain to process the image because one eye is not
} distracted.
}
} I am not a scientist, and would be interested in any corrections to the
} above.
}
} John W. Raffensperger, Jr.
} IS Manager
} Helwig Carbon Products, Inc.
}
}
} Email: gbarclay-at-trinidad.net
} Name: Greg Barclay
}
} Organization: University of the West Indies
}
} Education: Graduate College
}
} Location: St. Augustine, Trinidad, West Indies
}
} Question: Light microscope terminology: We all know what a
} stereomicroscope is and what a compound microscope is, but are they
} not technically both the same thing?
}
}


From daemon Tue Oct 15 07:32:27 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Tue, 15 Oct 2002 08:25:39 -0400
Subject: Re: en-block verses post-embedding staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To enhance contrast a bit we kept stock bottles of 50% and 70% etoh with
1-2% UA adding contrast while dehydrating. As long as tissue pieces were
small this worked well. Solution was drawn from the top or filtered to
prevent the precip that collected in the bottom. Time was doubled in these
two steps. Obviously if extraction or glycogen was important then this was
not done.

Scott Whittaker
Laboratories of Analytical Biology
Smithsonian Institution
National Museum of Natural History
PO Box 37012 MRC104
Washington DC 20013-7012
202-357-1651


} } } mohammed y abdulrawoof / f40z006 75760 {mdyousuf-at-KSU.EDU.SA} 10/10/02
12:35PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear listers,
Please advise as to the usefulness/advantage of doing enblock staining of
biological tissues as against doing heavy-metal staining of grids with
thin sections. The tissues that I am about to process are for diagnosis
(pathology).

Thanks
M. Yousuf Abdul-Rawoof




From daemon Tue Oct 15 07:44:49 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 15 Oct 2002 13:38:02 +0100 (GMT Daylight Time)
Subject: Re: ESEM vs. gold coated SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ESEM images of bacteria are different from gold coated
images of either air dried or CPD bacteria.

Firstly many bacteria produce slime. In air dried
samples this collapses. In CPD samples the solvents
probably remove it or condense it. In ESEM the slime is
retained so that one often does not see naked bacteria at
all. This conflict with expectation can lead to
disappointment!

Secondly, gold gives a good SE yield from the surface. In
ESEM (tungsten filament) bacteria yield less SE and from a
greater depth. This gives a "softer" image of bacteria than
one is used to.

Don't worry about coating units being outmoded. We showed
school visitors 7 samples today. All were gold coated!
The samples are easier to image.

With many dry materials samples it is easier to gold coat
and then image in ESEM. I know this is cheating ....

We are still using CPD for projects comparing preparation
methods.

Dave


On Thu, 10 Oct 2002 12:40:14 -0500 "Garber, Charles A."
{cgarber-at-2spi.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} David Patton wrote:
} ==========================================================
} Disclaimer: ESEM images of bacteria have a different quality to those that
} } have been dried and gold coated; so warn your user to avoid
} disappointment.
} ===========================================================
}
} Are you comparing ESEM images with a) air dried and gold coated or b)
} critical point dried and gold coated?
}
} It would be obvious that comparing air dried would indeed show a difference
} but if you really were comparing it to critical point dried samples, could
} you elaborate a bit on the nature of those differences? And would you
} have any comment about differences in contrast comparing ESEM vs. gold
} coated images.
}
}
} Disclaimer: SPI Supplies offers critical point dryers and sputter coaters so
} I guess we would be a bit nervous of any technology that should evolve that
} would render obsolete at the same time both CPD units and sputter coaters!
}
} Chuck
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.2spi.com
} ########################
} ============================================
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Oct 15 08:56:18 2002



From: George_Munzing-at-engelhard.com
Date: Tue, 15 Oct 2002 09:22:47 -0400
Subject: Need a high temperature epoxy for microtoming

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello fellow microscopists,

I need to do some high temperature TEM studies and am looking for a
suitable mounting resin. I will be embedding and microtoming powder
materials and would like to go as high in temperature as possible. The
materials themselves can withstand the temperatures but I doubt my
conventional epoxy would survive.

Is there any silicone epoxy or other suitable material designed to
withstand high temperatures (maybe as high as 600-800C) for a short time
for embedding?

Any suggestions you can offer are greatly appreciated.

Thanks in advance!

George R. Munzing Jr.
Engelhard Corporation
Strategic Technologies Group
25 Middlesex/Essex Turnpike
Iselin, NJ 08830
Tel: 732-205-7030
FAX: 732-205-5300



From daemon Tue Oct 15 10:21:49 2002



From: Shane Roberts :      roberts-at-southbaytech.com
Date: Tue, 15 Oct 2002 08:02:58 -0700
Subject: Glue for TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is another 'glue' that allows you to tailor the ion milling rate. The
product is called Araldit or LEIT-C. It is a carbon based glue that starts
as a powder, transitions into a viscous liquid, then polymerizes shortly
thereafter. The advantage is the glue mills at a slightly lower rate than
normal epoxies, and you can increase the carbon content to further reduce
the milling rate. South Bay Technology does supply this type of product.
Please let me know if you would like further information.

Best Regards,
----------------------------------
Shane Roberts
Applications Engineer
South Bay Technology,Inc.
'Materials Processing Solutions'
www.southbaytech.com
phone: 949.492.2600
fax: 949.492.1499
email: roberts-at-southbaytech.com
-----------------------------------

-----Original Message-----
} From: Isabel Nogueira [mailto:isabeln-at-popsrv.ist.utl.pt]
Sent: Monday, October 14, 2002 9:16 AM
To: Microscopy-at-sparc5.microscopy.com


Hello,

I need to prepare TEM cross-section samples of alternate SiO2 and TiO2
layers on a Si substrate. For that purpose I would appreciate suggestions on
glues, suitable for TEM work, that have a sputtering rate not too different
from my non homogeneous layers. The final sputtering will be on an Ion Mill
(4keV) using Ar+ ions.
All suggestions are welcome (including vendors) either to the list or to me
directly.
Thank you,

Isabel



Isabel Nogueira
Instituto Superior Técnico
Dep. Materiais
Avenida Rovisco Pais
1049-001 Lisboa
Portugal
tel.: +351 218418123
fax: +351 218418120
email: isabeln-at-popsrv.ist.utl.pt





From daemon Tue Oct 15 10:31:04 2002



From: mohammed y abdulrawoof / f40z006 75760 :      mdyousuf-at-KSU.EDU.SA
Date: Tue, 15 Oct 2002 18:17:07 -0300 (GMT)
Subject: Thanks: en-block verses post-embedding staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All who replied,
Using both en block staining for the tissues followed by double staining
of grids with UA and Lead citrate seems to be a good idea. Anyway, to get
a personal insight and experience, I will try fixation with and without
en-block staining.
Thanks again.
M. Yousuf.




From daemon Tue Oct 15 15:27:40 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Tue, 15 Oct 2002 16:14:45 -0400
Subject: RE: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I saw the first post this weekend and am jut now getting to it - doesn't
look like the answer is really been quite addressed yet... and yes even
at the risk of starting something . . . . ;)

What a wonderful question. Another semantic, logical, debatable but
ultimately one answer question!

Compound microscopes are as you say anything with a compound series of
lenses. Yes a 'Stereo' microscope should be called a compound stereo
microscope (or some variation). The distinction is that (I feel) the
"compound" microscope should be call a transmitted (or epi-illuminated)
light microscope. But then that would demonstrate an inclination of an
electron microscope bias.

There is nothing wrong with the term stereomicroscope, but the issue
with changing the compound microscope's name is more difficult than
worth it. It is much like trying to get the rest of the US to start
using the metric system. The terminology is too entrenched, but it
works, so why fix it?

The term binocular then becomes similarly confusing as Stereo
microscopes are also 'binocular' the fixed definition isn't much better
than the 'compound' in that it has the same issues. But then as
addressed before binocular compound 'transmitted' microscopes are not
'stereo' microscopes.

Greg, call 'em what you want - as long as your definition is made clear
to your audience and the distinctions made in the discussion on the list
server is kept in mind.

Geoff Williams
Microscopy Facility Supervisor


} -----Original Message-----
} From: by way of Ask-A-Microscopist [mailto:gbarclay-at-trinidad.net]
} Sent: Saturday, October 12, 2002 2:48 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist:LM terminology
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (gbarclay-at-trinidad.net) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
} October 11, 2002 at 23:40:22
}
------------------------------------------------------------------------
--
} -
}
} Email: gbarclay-at-trinidad.net
} Name: Greg Barclay
}
} Organization: University of the West Indies
}
} Education: Graduate College
}
} Location: St. Augustine, Trinidad, West Indies
}
} Question: Light microscope terminology: We all know what a
} stereomicroscope is and what a compound microscope is, but are they
} not technically both the same thing? Should not the distinction be
} between the "simple microscope," basically a highly corrected doublet
} or triplet lens, forming the Dowland type of microscope, and any kind
} of microscope with a separate eyepiece and objective lens? If so,
} then both the stereo and binocular (or monocular) microscopes are
} compond microscopes. I have been referring to "stereomicroscopes and
} binocular microscopes" in our Life Sciences Dept, and having some
} arguments with those who refer "stereomicroscopes and compound
} microscopes," over what is really what.
}
}
------------------------------------------------------------------------
--
} -



From daemon Wed Oct 16 02:09:08 2002



From: wang lan :      wins-at-public.xa.sn.cn
Date: Wed, 16 Oct 2002 14:47:15 +0800
Subject: optical products binocurlas, microscopes, telescopes, riflescopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We make new optical instruments for importers:

www.winsbinoculars.com

Regards,


Wentao


From daemon Wed Oct 16 02:09:08 2002



From: wang lan :      wins-at-public.xa.sn.cn
Date: Wed, 16 Oct 2002 14:53:13 +0800
Subject: optical products binocurlas, microscopes, telescopes, riflescopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We make new optical instruments for importers:

www.winsbinoculars.com

Regards,


Wentao


From daemon Wed Oct 16 08:23:00 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Wed, 16 Oct 2002 14:11:40 +0100
Subject: WORM drives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,
although this is strictly not a microscopy question, I'm posting
this in the hope that someone has been through this process already and can
offer some advice.

Our LEO 360FE SEM computer saves images on a Panasonic LF-7300 WORM drive.
It doesn't seem feasible to connect the computer to our intranet directly
(too old/difficult!) but a relatively straighforward way to get at the
images is to install an identical WORM drive on a PC which is networked.
However I imagine that some kind of software is needed to make the drive
functional under Windows NT. My internet searches for drivers have drawn
blanks so far. Has anyone done this and can give me some advice?
We are asking LEO for some support on this but independent advice is
always very valuable.

Many thanks,

Richard


_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com



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From daemon Wed Oct 16 13:08:48 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 16 Oct 2002 07:54:23 -1000 (HST)
Subject: Foil covered SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all-

Here is a colleague's method for using aluminum foil on SEM stubs for a
smooth background:

1. "Reynolds" seems smoothest and "regular" seems easiest to work with.

2. Burnishing. I've tried various things and they usually end up
scratching the foil. To make sure it is absolutely smooth flat, I
carefully cut a strip that is a bit wider and longer than a stub.
Place it on a clean surface, cover it with something very smooth
(weighing paper works well) and work over it with a finger tip to get
it absolutely as flat as possible. Picking it up by the very edge
with tweezers helps avoid wrinkles.

3. The smooth, flat piece is then transferred to a piece of
double-stick scotch tape and once more pressed to the tape to get a
very flat surface with no bubbles between foil and tape. This should
be done on glass or a surface from which tape plus foil can then be
carefully lifted.

4. The tape plus foil can then be applied to the stub - if it still
looks nice, proceed to step five. If wrinkles or bumps have appeared,
pull it up and try again. (You can put the tape directly on the stub
and then apply the foil - However, I have found that it works best
for me to have a nicely bonded duo of smooth tape and foil first)

5. Using weighing paper, again, press tape plus foil gently to the stub.

6. Finally, use a single-edge razor blade to trim off the excess foil
and tape around the edge of the stub. You can apply a couple of teeny
dabs of silver paste at several edge points if you want to make
absolutely certain the foil is well connected to the stub. I have
gotten into the habit of making the strip of foil plus tape slightly
narrower than stub diameter so that there is a small crescent of stub
above and below the strip, simply as a way of telling top and bottom
at a glance.

7. Additional note. For mounting an object with a convex (like my
little larval shells) or irregular lower surface, aluminum foil can
be gouged with a minuten insect pin before applying silver paste or
paint to help achieve better contact and bonding.

As with everything, it just takes a bit of experimentation. . . .


I hope this helps!

Aloha,
Tina



From daemon Wed Oct 16 13:15:00 2002



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Wed, 16 Oct 2002 13:08:25 -0500
Subject: Re: Ask-A-Microscopist:epi-fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Sundeep,
If you try entering "polarized fluorescence microscopy" into the Google
search engine you will get some hits. Using a polarized light source in
conjunction with a crossed polarizing filter which can be rotated
(analyzing filter) is a technique used in different approaches to
fluorescence microscopy to observe molecular rotations in protein folding
or in TIRF applications. It can be used in conjunction with FRET type
experiments. I believe the book entitled "Fluorescence Microscopy"
published by Bios Scientific may have a short section which discusses
polarized fluorescence microscopy.
Regards,
Karl G.

At 06:11 PM 10/10/2002 -0500, sundeep.bhandari-at-nikon.co.uk wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Wed Oct 16 13:43:17 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 16 Oct 2002 14:41:15 -0400
Subject: RE: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike & Co.

We also have an Olympus Model SZH10 that has only one "objective" but it's
not really an objective lens [1X to 80X total mag. for microscope]. It's
simply a mag. doubler in front of the pair of objective lenses. If one
turns the microscope upside down and removes this mag. doubler, they would
see two objectives and there are two optical paths all the way up to the
eyepieces. Ain't no way to have stereo with one optical path. I've never
seen a "metallurgical" "compound" microscope that has mags. up to 1 KX. with
two distinct optical paths.



Peter

-----Original Message-----
} From: Mike Bode [mailto:mb-at-Soft-Imaging.com]
Sent: Monday, October 14, 2002 4:36 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


I think, Peter and you are right with the statement that different light
paths are necessary to achive a true stereo effect. I am not so sure, that 2
separate objectives are really required. I know of at least one company (I
have no affiliation with or interest in that company) that makes a 3D
microscope and I think they are using only one objective lens
(http://www.edge-3d.com).

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: John W. Raffensperger, Jr. [mailto:johnr-at-helwigcp.com]
Sent: Monday, October 14, 2002 8:59 AM
To: 'by way of Ask-A-Microscopist'; Microscopy-at-sparc5.microscopy.com


Just because a microscope has two eyepieces, does not mean that it is a
"stereo" microscope.

A stereo microscope has not only two eyepieces, but also two objectives,
and two separate light paths. Because of having two slightly offset
images, you see a three dimensional image. Usually these are lower
powered systems, but still compound microscopes (multiple lens systems).

On a high power (relatively) microscope with a binocular head, you only
have one objective, and one light path from the specimen to the head.
The prism(s) in the head divide the light path to provide multiple
images, but they are all (three in the case of an extra camera port)
identical. This does not provide a 3-D image, it just makes it easier
on the eyes and brain to process the image because one eye is not
distracted.

I am not a scientist, and would be interested in any corrections to the
above.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.


Email: gbarclay-at-trinidad.net
Name: Greg Barclay

Organization: University of the West Indies

Education: Graduate College

Location: St. Augustine, Trinidad, West Indies

Question: Light microscope terminology: We all know what a
stereomicroscope is and what a compound microscope is, but are they
not technically both the same thing?



From daemon Wed Oct 16 13:52:43 2002



From: akc-at-umich.edu
Date: Wed, 16 Oct 2002 14:46:01 -0400
Subject: RE: Ask-A-Microscopist:LM terminology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the history of microscopy, the term "compound microscope" was used in
contrast to a "simple microscope." A simple microscope had one lens, while
a compound microscope had two or more lenses (for example, an eyepiece).
Thus in the 1600s, Leeuwenhoek used a simple microscope (one lens of rather
high magnification), while Hooke did his work with a compound microscope
(which had an eyepiece). Compound microscopes were much more convenient to
use and were easier on the eyes. On the other hand, simple microscopes
were less affected by chromatic abberation, which allowed Leeuwenhoek to
see smaller objects (bacterial, sperm, and so forth) than could be seen
with the compound microscopes of the time. Not until the 1820s, with the
advent of achromatic lenses for compound microscopes, could microscopists
improve much on the detail of Leeuwenhoek's observations.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Tuesday, October 15, 2002 4:14 PM -0400 Geoff Williams
{willi1gl-at-cmich.edu} wrote:

} I saw the first post this weekend and am jut now getting to it - doesn't
} look like the answer is really been quite addressed yet... and yes even
} at the risk of starting something . . . . ;)
}
} What a wonderful question. Another semantic, logical, debatable but
} ultimately one answer question!
}
} Compound microscopes are as you say anything with a compound series of
} lenses. Yes a 'Stereo' microscope should be called a compound stereo
} microscope (or some variation). The distinction is that (I feel) the
} "compound" microscope should be call a transmitted (or epi-illuminated)
} light microscope. But then that would demonstrate an inclination of an
} electron microscope bias.
}
} There is nothing wrong with the term stereomicroscope, but the issue
} with changing the compound microscope's name is more difficult than
} worth it. It is much like trying to get the rest of the US to start
} using the metric system. The terminology is too entrenched, but it
} works, so why fix it?
}
} The term binocular then becomes similarly confusing as Stereo
} microscopes are also 'binocular' the fixed definition isn't much better
} than the 'compound' in that it has the same issues. But then as
} addressed before binocular compound 'transmitted' microscopes are not
} 'stereo' microscopes.
}
} Greg, call 'em what you want - as long as your definition is made clear
} to your audience and the distinctions made in the discussion on the list
} server is kept in mind.
}
} Geoff Williams
} Microscopy Facility Supervisor
}
}
} } -----Original Message-----
} } From: by way of Ask-A-Microscopist [mailto:gbarclay-at-trinidad.net]
} } Sent: Saturday, October 12, 2002 2:48 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Ask-A-Microscopist:LM terminology
} }
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (gbarclay-at-trinidad.net) from
} } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
} } October 11, 2002 at 23:40:22
} }
} ------------------------------------------------------------------------
} --
} } -
} }
} } Email: gbarclay-at-trinidad.net
} } Name: Greg Barclay
} }
} } Organization: University of the West Indies
} }
} } Education: Graduate College
} }
} } Location: St. Augustine, Trinidad, West Indies
} }
} } Question: Light microscope terminology: We all know what a
} } stereomicroscope is and what a compound microscope is, but are they
} } not technically both the same thing? Should not the distinction be
} } between the "simple microscope," basically a highly corrected doublet
} } or triplet lens, forming the Dowland type of microscope, and any kind
} } of microscope with a separate eyepiece and objective lens? If so,
} } then both the stereo and binocular (or monocular) microscopes are
} } compond microscopes. I have been referring to "stereomicroscopes and
} } binocular microscopes" in our Life Sciences Dept, and having some
} } arguments with those who refer "stereomicroscopes and compound
} } microscopes," over what is really what.
} }
} }
} ------------------------------------------------------------------------
} --
} } -
}








From daemon Wed Oct 16 15:14:00 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Wed, 16 Oct 2002 15:56:44 -0400
Subject: Brain Fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

Does anyone have a recipe for mouse brain perfusion and fixation? I am
trying to get the best brain preservation in mice. I trying to prevent
neurons from swelling and I need to look at the Golgi apparatus. How about
calcium chloride as an additive?

Thanks!

Karen

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954



From daemon Wed Oct 16 18:23:03 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 16 Oct 2002 18:07:31 -0500
Subject: WORM drives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If your drive is the old WORM technology, good luck finding one! I haven't
seen one for sale in years.

What computer does the 360 use? I have forgotten... Even if it is a DEC
(perhaps especially) it would be easier to send the data to a newer PC for
storage on hard drives and CD-Rs.

Regards, Woody

Woody White
McDermott Technology Inc.
McD: http://www.mtiresearch.com/
Mine: http://woody.white.home.att.net


-----Original Message-----
} From: Richard Beanland [mailto:richard.beanland-at-bookham.com]
Sent: Wednesday, October 16, 2002 9:12 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Hello everyone,
although this is strictly not a microscopy question, I'm posting
this in the hope that someone has been through this process already and can
offer some advice.

Our LEO 360FE SEM computer saves images on a Panasonic LF-7300 WORM drive.
It doesn't seem feasible to connect the computer to our intranet directly
(too old/difficult!) but a relatively straighforward way to get at the
images is to install an identical WORM drive on a PC which is networked.
However I imagine that some kind of software is needed to make the drive
functional under Windows NT. My internet searches for drivers have drawn
blanks so far. Has anyone done this and can give me some advice?
We are asking LEO for some support on this but independent advice is
always very valuable.

Many thanks,

Richard


_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com



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From daemon Thu Oct 17 03:17:23 2002



From: Peter Heimann :      peter.heimann-at-uni-bielefeld.de
Date: Thu, 17 Oct 2002 09:48:24 +0200
Subject: Re: Brain Fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Karen:

from my experience with mouse (CNS; spinal chord, muscle, testis
and other tissue) I would strongly recommend a mixture of 2%
paraformaldehyde / 4% glutaraldehyde (made from "the good one" prepared
after"Anderson")and a short (!) pre-perfuse (as you name it) to flush
out all blood cells, which otherwise regularly clog and occlude the fine
capillaries.
However, though simple saline flush can be sufficient, I routinely
use a mixture of Heparin (anti-coagulant), Procain (keeps the blood
vessels "open"), PVP (polyvinylpyrrolidon, MW-class 30 K; for
osmotic pressure) in aqua dest. and pH-adjustment.
Method published originally in a wonderful perfusion-technique
paper by Forssmann, WG et al (1977) in Anat. Rec. 188: p 307-
314. Just follow their technical procedure exactly (except that in the
small mouse you have to use a 26 gauge butterfly teflon-needle) and
you`ll get excellent fixation.
good luck!
peter
**********************************
peter.heimann-at-uni-bielefeld.de
Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : " " - 5654
www.uni-bielefeld.de/biologie/Entwicklungsbiologie/
www.uni-bielefeld.de/SFB549
***********************************



From daemon Thu Oct 17 03:22:06 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 17 Oct 2002 01:21:08 -0700
Subject: Re: Brain Fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,

I perfuse mouse with 1x PBS (20 mM Na-phosphate, 150 mM NaCl, PH 7.4) 15
ml. 2 ml/min, room temp, then same amount/speed 0.1% GA + 2% formaldhyde in
1x PBS, room temp. Brain as quick as possible removed from the scull on ice
and sliced 0.5 mm thick. Slices individually fixed in 1% GA/2%
formaldehyde/1x PBS at +4oC overnight.

I did not check Golgi but mitochondria and other stuff is OK. Sergey

P.S. I did not try Ca+2, but it's good combination with formaldehyde for
brain.

P.P.S. All chemicals are EM grade and fixers prepared just before
use. Slicing is critical. Speed of preparation is VERY critical. The way
you kill animal is also critical. Godd luck!

At 03:56 PM 10/16/02 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Oct 17 05:00:38 2002



From: Albert Coritz :      cactusgrower-at-earthlink.net
Date: Thu, 17 Oct 2002 05:52:15 -0400
Subject: Help with Decalcification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone:

Just passing this along for Matt who is not on the listserver. Please
email him directly with any ideas.

Many Thanks!

Al Coritz
Electron Microscopy Sciences

I was wondering if you knew any good protocols for decalcifying the
mouse skull. We haven't done too much of it, but when we have, we've
used 3%HCl and 15%NaCl.
As always, we're looking for a way to keep shrinkage of the brain to a
minimum. Do you have any ideas? Any help would be appreciated.
Thanks a lot.
Matt

Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
mcelwee-at-surgery.wisc.edu



From daemon Thu Oct 17 07:02:39 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Thu, 17 Oct 2002 07:50:54 -0400
Subject: Source Code for Image Processing Functions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a collection of highly optimized source code (in C) for the
basic image processing functions.

Everett Ramer
Cellomics, Inc.
Pittsburgh, PA


From daemon Thu Oct 17 08:12:54 2002



From: Pijpers,Frank :      fpijpers-at-nl.feico.com
Date: Thu, 17 Oct 2002 15:04:13 +0200
Subject: RE: Source Code for Image Processing Functions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} -----Original Message-----
} From: Everett Ramer [mailto:eramer-at-cellomics.com]
} Subject: Source Code for Image Processing Functions

You may want to have a look at Intel's IPP (http://www.intel.com/software/products/ipp/ipp30/index.htm).

Sincerely,

Frank Pijpers
---
TEM Product Architect FEI
mailto:fpijpers-at-nl.feico.com


From daemon Thu Oct 17 09:05:47 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Thu, 17 Oct 2002 09:49:48 -0400
Subject: Re: Brain Fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,
Shrinkage or swelling of course is related to the osmolarity of the fix
(and vehicle). I like to run a
variety of fixes ie 2.5% GA vs 2% PF + 1.25% GA, etc. I think a good buffer
would be 0.1 M Hepes and
definitely add 3 mM CaCl2 for membrane tonicity. When osmicating it is
important to use KFECN6 (0.8%) reduced
osmium (1%), this keep the myelin sheaths from blebing (on ice). I also keep
the CaCl2 in the osmium step. I also
like long en-bloc staining (2% filtered UA for 2 hrs). This gives good
contrast and helps stabilize membranes during
dehydration. Remember even though you perfused to cut small pieces (3 mm
cubed) for infiltration of subsequnent solutions.
If you want to see actin or neurofilaments use 0.15% tannic acid after the
osmium (about 5 min only)-Kent McDonald-
With tissue I like to go 1:1 Propylene Oxide to Epon overnight. Good Luck.

Mike Delannoy
JHMI Microscopy Facility

"Jensen, Karen" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers:
}
} Does anyone have a recipe for mouse brain perfusion and fixation? I am
} trying to get the best brain preservation in mice. I trying to prevent
} neurons from swelling and I need to look at the Golgi apparatus. How about
} calcium chloride as an additive?
}
} Thanks!
}
} Karen
}
} Karen L. Bentley, M.S.(previously Jensen)
} Associate Scientist & Project Manager
} Electron Microscope Research Core
} University of Rochester Medical Center
} Rochester, NY 14642
} 585-275-1954



From daemon Thu Oct 17 09:17:10 2002



From: Ssjh1818-at-aol.com
Date: Thu, 17 Oct 2002 11:24:20 -0400
Subject: RE: Source Code for Image Processing Functions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might also check this page http://sourceforge.net/

Pavel

----- Original Message -----
} From: "Pijpers,Frank" {fpijpers-at-nl.feico.com}
To: "Microscopy-at-MSA.Microscopy.Com" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, October 17, 2002 9:04 AM


we have an agfa duoscan T2500. we would like to better automate our scanning. does anyone know of a method of auto scaning negs on the duoscan.
thank s
john



From daemon Thu Oct 17 10:31:25 2002



From: Ssjh1818-at-aol.com
Date: Thu, 17 Oct 2002 11:22:08 -0400
Subject: Re: batch scanning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


we have an agfa duoscan T2500. we would like to better automate our scanning. does anyone know of a method of auto scaning negs on the duoscan.
thank s
john


From daemon Thu Oct 17 11:18:31 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Thu, 17 Oct 2002 11:10:06 -0500
Subject: Antivibration platform

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We are going to move our EM lab to a new place
(in the same building) and are planning to put an
anti-vibration platform under the column of our
FEG-ESEM XL30. Do we have to place a HV transformer
on the same platform (the transformer is right behind
the column)?

Any additional advise will be very helpful also,
especially experience with anti-vibration platforms
of different manufacturers.

Right now I can routinely make micrographs at magnifications
only up to 50,000. After hours, when vibrations are lower,
I can (sometimes) go up to 100,000-150,000. Can platform
improve performance of microscope in this range of magnifications?

Thank you,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy




From daemon Thu Oct 17 14:30:52 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 17 Oct 2002 15:20:24 -0400
Subject: Fwd: Re: batch scanning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
}
} FotoLook has to be run as an independent application (not from within
} Photoshop).
} Batches can be defined here.
} To repeat the same batch, drag the contents of the Done folder to the ToDO
} folder and run the batch again.
}
} At 11:22 AM 10/17/2002 -0400, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/




From daemon Thu Oct 17 15:32:18 2002



From: Rick Hugo :      hugo-at-pdx.edu
Date: Thu, 17 Oct 2002 13:24:03 -0700
Subject: Technics MIM-IV ion mill parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all - anybody have spare parts for the Technics MIM-IV ion mill, or
know where I can find them? The anode tips in our ion guns (TSA 1-aperture)
are pretty hopelessly worn out. Any help would be greatly appreciated!

Rick

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Rick Hugo, Ph.D.
Research Associate, Geomicrobiology and Electron Microscopy Lab
Department of Geology, Portland State University
Portland, OR 97207-0751
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}



From daemon Thu Oct 17 15:45:46 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Thu, 17 Oct 2002 16:38:54 -0400
Subject: Re: Source Code for Image Processing Functions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 07:50 AM 10/17/02 -0400, Everett Ramer {eramer-at-cellomics.com} wrote:
} I am looking for a collection of highly optimized source code (in C) for the
} basic image processing functions.


Optimized for what?



**Please note new department name**

Kevin Frischmann, Laboratory Manager
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: 212-313-7975
Fax: 212-496-3480
email: kfrisch-at-amnh.org



From daemon Thu Oct 17 16:01:28 2002



From: saram-at-duke.edu
Date: Thu, 17 Oct 2002 16:50:49 -0400 (EDT)
Subject: Re: en-block verses post-embedding staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gang Ning is partially right.

For what it's worth:

UA is soluble in veranol acetate buffer at pH 4.5 and below.

However, UA is INSOLUBLE in cacodylate buffer at 4.5 and 3.5.

UA is soluble in cacodylate buffer at pH 0 and 0.5.

I don't know what happens between 0.5 and 3.5, but who wants to use a
buffer at that pH anyway? Somebody may have publised this info, but I
really don't need to know enough to chase it down. If one wanted to
stain at that low pH, cacodylate doesn't have much buffering capacity
down there.

Don't quote me on the exact pH's. I just tried this with pH paper and
tiny drops, not a pH meter.

I still recommend washing out the caco with the buffer that the UA is in
(or water) before using adding UA---that is if you choose to do en bloc
staining.

Sara Miller



On Tue, 15 Oct 2002, gang ning wrote:

} One thing I want to point out is people usually don't use UA in
} cacodylate buffer is NOT for the reason that UA is NOT SOLUBLE in it,
} but for the reason that UA works better in buffers of low pH.
}
} saram-at-duke.edu wrote:
}
} } Again, see CAPS below.
} } (CAPS ARE USED TO DIFFERENTIATE COMMENTS, NOT TO CONVEY HOSTILITY.)
} }
} }
} } On Sun, 13 Oct 2002, gang ning wrote:
} }
} } } Thanks for the clear definition of biohazard. But it is obvious that
} } } 1) UA is a nasty/hazard chemical. Everyone wants to avoid it if possible,
} } }
} }
} } UA CERTAINLY CAN BE CALLED NASTY. IT DOES REQUIRE SPECIAL HANDLING
} } PROCEDURES, AS DO OTHER CHEMICALS THAT WE USE AND CAN BE HANDLED SAFELY
} } UNDER THE PROPER CONDITIONS. NOT EVERYONE WANTS TO AVOID IT. FOLKS MUST
} } MAKE THEIR OWN JUDGEMENT AS TO WHETHER THE EXTRA PROCESSING TIME AND CARE
} } OF HANDLING ANOTHER CHEMICAL IS WORTH IT TO THEM. NOT USING UA IS FINE.
} } HOWEVER, DO NOT MAKE A GENERALIZATION ABOUT *EVERYONE* JUST BECAUSE YOU
} } CHOOSE NOT TO USE IT.
} }
} } } 2) it does take at least 1 hr + time for washing thoroughly (how long?)
} } } for a 1 mm3 block .... it is clearly time-consuming procedure
} } }
} }
} } IT DOES REQUIRE EXTRA TIME (1-2 HOURS). ONE CAN STAIN A 1 mm3 BLOCK FOR
} } 30-60 MIN AND WASH FOR 30-60 MIN.
} }
} } unless your turn around time is not an issue and working overtime is not a
} }
} } } matter with your hospital policy,
} } }
} }
} } TIME IS VERY MUCH AN ISSUE FOR US IN THE HOSPITAL SETTING. OUR TURN
} } AROUND TIME IS *ROUTINELY* NEXT DAY FOR SAMPLES FIXED IN GLUT, OS, UA.
} } TISSUE COMES IN IN THE AM, IS PROCESSED THAT DAY, BAKED OVERNIGHT, CUT AND
} } VIEWED THE NEXT DAY, AND PATHOLOGISTS HAVE MICROGRAPHS *IN HAND* THAT
} } AFTERNOON--ALMOST ALWAYS. MY TECHS RARELY HAVE TO WORK OVERTIME, AND WHEN
} } THEY DO, IT'S USUALLY BECAUSE OF EXTRA CUTTING OF HAVING TO RETRIEVE
} } SPECIMENS OUT OF PARAFFIN BLOCKS OR OFF SLIDES, NOT BECAUSE THEY PUT
} } SOMETHING INTO UA.
} }
} } YOU ARE CLEARLY VERY NEGATIVE TOWARD THE USE OF UA. FINE. DON'T USE IT.
} } BUT DON'T BE SARCASTIC ABOUT SPECIMEN TURN AROUND TIME AND
} } TECHNICIAN OVERTIME, BECAUSE IT JUST ISN'T AN ISSUE IF SPECIMENS AND
} } WORKLOADS ARE MANAGED PROPERLY.
} }
} } } 3) en block staining has adversary effect on showing glycogen so is not
} } } suitable for muscle, heart, liver and so on; there is not significant
} } } meaning for kidney .. then what else left? LOTS:
} } }
} }
} } BRAIN, CILIA, TUMORS, VIRUSES, MUSCLE DISEASES OTHER THAN GLYCOGEN STORAGE
} } DISEASES, RESEARCH SAMPLES, ETC.
} }
} }
} } It is overkill for solid tissue blocks, IT IS NOT OVERKILL WHEN THE
} } BEST PRESERVATION, RATHER THAN DECREASING PROCESSING TIME IS OF
} } IMPORTANCE.
} }
} } and 4) ethanolic/methanolic UA of higher concentration penetrates tissue
} }
} } } much faster than other aqueous UA solutions and gives fresher image and
} } } better contrast but sometimes leave precipitation around cell member no
} } } matter how one washes before staining.
} } }
} }
} } AS I SAID BEFORE, IF THE CORRECT BUFFER IS USED SO THAT UA DOES NOT
} } PRECIPTATE WITH IT, THERE IS NO PRECIPITATION. PERHAPS YOUR *HIGHER
} } CONCENTRATION* IS A CAUSE OF DIFFICULTY IN WASHING. SINCE UA IS SOLUBLE
} } IN ALCOHOL, UNBOUND, UNPRECIPITATED UA IS EASILY WASHED OUT BY THE
} } DEHYDRATING AGENT(S). IF YOU HAVE PRECIPITATE, IT IS MOST LIKELY DUE TO
} } SOME REACTION WITH THE SOLUTION LEFT IN THE CELL BEFORE YOU ADD THE UA, OR
} } PERHAPS THE *HIGHER* CONCENTRATION YOU USE ISN'T WASHED OUT???
} }
} } } For EM work, the simpler and better, as long as it works.
} } }
} }
} } YES, THE KEY IS, *AS LONG AS IT WORKS* FOR THE PURPOSE YOU HAVE. IF THE
} } PURPOSE IS TO PRODUCE THE BEST ULTRASTRUCTURE, AND IF MEMBRANES AND OR
} } LIPIDS ARE PART OF THE QUESTION, USE OF UA IS GOOD.
} }
} } Overkill is always a problem. YES, NO ONE WANTS TO SPEND MORE TIME THAN
} } IS NECESSARY OR USE CHEMICALS THAT CAN BE HAZARDOUS IF NOT HANDLED
} } PROPERLY, BUT UA IS NOT ALWAYS OVERKILL.
} }
} } IT DEPENDS ON YOUR APPLICATION. EACH TO HIS OWN.
} }
} } }
} } }
} } } saram-at-duke.edu {mailto:saram-at-duke.edu} wrote:
} } }
} } } } See CAPS interspersed below for distinction from the original.
} } } }
} } } } Sara Miller
} } } }
} } } } On Fri, 11 Oct 2002, Gang Ning wrote:
} } } }
} } } } } ------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
} } } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } -----------------------------------------------------------------------.
} } } } }
} } } } }
} } } } } Hi
} } } } }
} } } } } I agree with Dr. Sara Miller's point that UA en block staining in some extent
} } } } } helps in preservation of ultrastructure as well as enhancement of the
} } } } } contrast. However, it also has disadvantages such as one has to deal with UA,
} } } } } a biohazard reagent,
} } } } }
} } } } A BIOHAZARD IS A BIOLOGICAL AGENT THAT IS HAZARDOUS (INFECTIOUS). UA IS
} } } } RADIOACTIVE AND IS A HEAVY METAL. IT, LIKE GLUTARALDEHYDE, OSMIUM, LEAD,
} } } } RESINS, PROPYLENE OXIDE, ETC., SHOULD BE HANDLED WITH CARE. HANDLED
} } } } PROPERLY, IT IS NOT ANY MORE DANGEROUS THAN THE OTHER NASTY CHEMICALS
} } } } ELECTRON MICROSCOPISTS HAVE TO USE. IT IS NOT A BIOLOGICAL AGENT.
} } } }
} } } } and sometimes background from crystal precipitation,
} } } }
} } } } } especially when ethanolic UA is used.
} } } } }
} } } } WE NEVER SEE PRECIPITATION. PERHAPS TRY DILUTING THE STAIN OR WASHING
} } } } THOROUGHLY BEFORE USING IT WILL ELIMINATE THE PRECIPITATE. UA IS NOT
} } } } SOLUBLE IN PHOSPHATE OR CACODYLATE BUFFER; IF YOU DON'T WASH OUT THESE
} } } } BUFFERS BEFORE ADDING UA IN VERANAL ACETATE BUFFER, UA IN WATER, OR UA IN
} } } } ETHANOL OR METHANOL, YOU CAN GET PRECIPATE.
} } } }
} } } } More importantly, it is time-consuming
} } } }
} } } } } because it takes hours for aqueous UA solution to penetrate a sizable tissue
} } } } } block.
} } } } }
} } } } WE USE 1 MM TISSUE BLOCKS AND STAIN FOR AN HOUR 1% UA IN 0.11M VERONAL
} } } } ACETATE BUFFER. IF THE TISSUE OR CELL PELLET IS SMALLER, YOU CAN GET AWAY
} } } } WITH 30 MIN IN UA.
} } } }
} } } } Therefore, I would say it all depends your tissue and purpose of your
} } } }
} } } } } analysis.
} } } } }
} } } } I AGREE. IT ALL DEPENDS ON YOUR PURPOSE. IF YOU WANT BEAUTIFUL
} } } } ULTRASTRUCTURE AND YOU'RE NOT LOOKING SPECIFICALLY FOR GLYCOGEN, USE UA EN
} } } } BLOCK. IF YOU'RE IN A HURRY OR YOU CARE TO SEE ALL THE GLYGOGEN, DON'T
} } } } USE IT.
} } } }
} } } } } In my lab, we routinely do en block with samples of blood, tissue culture,
} } } } } virus, bacteria and other microorganisms, which are believed more vulnerable
} } } } } to solvent, but not with solid tissue. We have a lot of samples from kidney
} } } } } biopsy and we didn't find en block made any difference from staining thin
} } } } } sections whereas taking your time to have a sufficient osmication is much
} } } } } more helpful to your structure.
} } } } }
} } } } } Greg Ning
} } } } }
} } } } }
} } } } }
} } } } } mohammed y abdulrawoof / f40z006 75760 wrote:
} } } } }
} } } } } } ------------------------------------------------------------------------
} } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
} } } } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } } -----------------------------------------------------------------------.
} } } } } }
} } } } } } Dear listers,
} } } } } } Please advise as to the usefulness/advantage of doing enblock staining of
} } } } } } biological tissues as against doing heavy-metal staining of grids with
} } } } } } thin sections. The tissues that I am about to process are for diagnosis
} } } } } } (pathology).
} } } } } }
} } } } } } Thanks
} } } } } } M. Yousuf Abdul-Rawoof
} } } } } }
} } } } } --
} } } } } Gang Ning, M.D., Ph.D.
} } } } } Director
} } } } } Electron Microscopy Facility
} } } } } Medical College of Wisconsin
} } } } } 8701 Watertown Plank Road
} } } } } Milwaukee, Wisconsin 53226
} } } } } (414) 456-8344
} } } } } e-mail: gning-at-mcw.edu {mailto:gning-at-mcw.edu}
} } } } } http://www.mcw.edu/cellbio/emfacility.html
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } Sara E. Miller, Ph. D.
} } } } P. O. Box 3712
} } } } Duke University Medical Center
} } } } Durham, NC 27710
} } } } Ph: 919 684-3452
} } } } FAX: 919 684-3265
} } } }
} } } }
} } } --
} } }
} } } Gang Ning, M.D., Ph.D.
} } }
} } } Director
} } }
} } } Electron Microscopy Facility
} } }
} } } Medical College of Wisconsin
} } }
} } } 8701 Watertown Plank Road
} } }
} } } Milwaukee, WI 53226
} } }
} } } (414) 456-8141
} } }
} } }
} } }
} } }
} }
} } Sara E. Miller, Ph. D.
} } P. O. Box 3712
} } Duke University Medical Center
} } Durham, NC 27710
} } Ph: 919 684-3452
} } FAX: 919 684-3265
} }
} }
}
} --
}
} Gang Ning, M.D., Ph.D.
}
} Director
}
} Electron Microscopy Facility
}
} Medical College of Wisconsin
}
} 8701 Watertown Plank Road
}
} Milwaukee, WI 53226
}
} (414) 456-8141
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Oct 18 02:52:41 2002



From: pvosta-at-unionbio-eu.com
Date: Fri, 18 Oct 2002 09:40:32 +0200
Subject: Re: Source Code for Image Processing Functions

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I have put together an extensive collection of links to all kinds of
microscopy and imaging resources on the WWW and there are several links
to software and source code repositories:

http://ourworld.compuserve.com/homepages/pvosta/pcrimg.htm

Best regards,

Peter Van Osta

==============================================
Everett Ramer wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am looking for a collection of highly optimized source code (in C) for the
} basic image processing functions.
}
} Everett Ramer
} Cellomics, Inc.
} Pittsburgh, PA


From daemon Fri Oct 18 05:27:56 2002



From: Kevin Mackenzie :      k.s.mackenzie-at-abdn.ac.uk
Date: Fri, 18 Oct 2002 11:10:31 +0100
Subject: 30th SCOTTISH MICROSCOPY SYMPOSIUM Wednesday 13th November

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Final Announcement

30th SCOTTISH MICROSCOPY SYMPOSIUM Wednesday 13th November 2002

Aberdeen Exhibition & Conference Centre, Bridge of Don, Aberdeen AB23 8BL

Registration Form available on web site
http://www.abdn.ac.uk/emunit/smg2002.htm

Programme

10.00-10.45 "Mechanical Interactions of Cells with Surfaces" by Mathis
Riehle (Glasgow University)

10.45-11.30 Applications of Microscopical Techniques in Forensic Science"
by Alison Crossley, (AEA Technology plc, Oxford)

11.30-11.45 "FTIR Microscopy of Human Trabecular Bone" by Alison Coates,
(University of Aberdeen)

11.45-12.00 "Techniques for visualisation of apoptosis in osteoclasts" by
Mike Rogers and F. Coxon (University of Aberdeen Medical School)

12.00-13.45 Luncheon, Trade Exhibition, Poster Display

13.45-14.30 "Creating, Viewing and Storing Large Image Data Fields" by Alan
Entwistle, (Ludwig Institute, University of London)

14.30-14.45 "Development of an Image Analysis Program for Measuring Bone
Resorption in vitro" by Rob van't Hof and Miep Helfrich (University of
Aberdeen Medical School)

14.45-15.00 "Microscopy of Tick Salivary Glands" Barry Stewart (Zoology,
University of Aberdeen)

15.00-15.30 TEA

15.30-16.15 "Microscopy and variant Creutzfeldt-Jakob (vCJD) disease" by
James Ironside, (CJD Surveillance Unit, Edinburgh)

16.15-16.30 'Iso-butanol/water: a new staining technique for light and
electron microscopy by Ian Roberts', (Scottish Crop Research Institute, Dundee)

16.30 Presentation of Prizes



Electron Microscope unit
School of Biological Sciences
University of Aberdeen
Aberdeen
AB24 2TZ

Tel 01224-272847
Fax 01224-272396
------------
Kevin Mackenzie
k.s.mackenzie-at-abdn.ac.uk



From daemon Fri Oct 18 09:44:51 2002



From: Fred Pearson :      eoptics-at-mcmail.cis.mcmaster.ca
Date: Fri, 18 Oct 2002 10:31:46 -0400 (EDT)
Subject: Electropolishing Solution required

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Dear Group:

I have the task of preparing TEM foils from 1 mm. O.D. 18K and 14K Gold
wire. I was successful in ion milling dimpled 50um section/foils in PIPS
at 4Kv., 4 degree angle. We are looking at the strain fields about 100nm.
precipitates. The ion milling caused damaged to the structure as indicated
by the ripple patterns in the material as viewed in the TEM. Obviously
this may/can alter our examination.

Next, I would like to try electropolishing the material if I can make
suitable foils for the polisher. We cold rolled the wire, and punched out
3 mm. disks to work with. Electropolishing is the next step to try.

What I am asking, does anyone know of an electropolishing solution for Au,
suitable for a Tenupol 5 or a Fischione machine?

Thanks in advance

Fred Pearson


********************************************************
Fred Pearson
Brockhouse Institute for Materials Research
McMaster University
1280 Main St. West
Hamilton, Ontario
Canada L8S 4M1


email: eoptics-at-mcmaster.ca
phone: (905) 525-9140 ext. 24609
fax: (905) 521-2773
********************************************************



From daemon Fri Oct 18 10:38:10 2002



From: saram-at-duke.edu
Date: Fri, 18 Oct 2002 11:27:00 -0400 (EDT)
Subject: Re: Prions and OsO4

Contents Retrieved from Microscopy Listserver Archives
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Dear Phil, et al.

Thanks for your comments, Phil.

Listers, I'm forwarding this answer to the list because of our
recent discussion on UA and the question posed by Phil about UA
inactivation of prions.

The question at a recent microscopy meeting concerned whether osmium
"killed" (inactivated) prions. It arose because of a question about how
specimens suspected of containing prion material (Creutzfeld-Jacob
Disease and other spongiform encephalapathies) should be handled in the EM
lab.

The discussion centered around osmium inactivation because it is a proven
fact that aldehydes DO NOT inactivate prions. Osmium is usually the next
step, and most EM folks use it; some do not use UA. The osmium
inactivation question was also visited on this net some years ago.

My answer was that some oxidizing agents (peracetic acid; Vet J 159:10,
2000) reduce activity of prions in brain, and thus, one might suspect
that osmium could act similarly. This article does say that many
oxidizing agents tried have no effect, and that the effect is related to
whether the prions are aggretated (the more aggregated, as in homogenized
specimens, the less inactivated). Peracetic acid works better on brain
than on homogenized tissue. However, I pointed out that in a personal
communication with both Drs. D. M. Taylor and Stanley Prusiner, these
experts simply said that the experiment of osmium inactivation hasn't been
done. We simply don't know. Also, they pointed out that we don't even
know whether binding up the prions in resin inactivates them. They
recommended working with gloves and collecting the "chips" from trimming
for incineration.

Phil has raised the question of whether UA would inactivate prions. His
reasoning has merrit. However, I would maintain that, like osmium, UA has
not been tested, and that some things that one would think would
inactivate prions don't (see ref above). Just because they are stained or
bound up doesn't necessarily mean they are inactive.

Radioactivity, even very strong doses, does not inactivate prions. As a
matter of fact, this is one reason why folks feel that prions don't
contain nucleic acids.

If histology labs have to handle brain suspected of containing prions,
they inactivate the tissue with 97% formic acid after formaldehyde
fixation (Neurology 40:887, 1990), which doesn't seem too detrimental to
structure.


Sara Miller


On Fri, 18 Oct 2002, Philip Oshel wrote:

} Sara,
}
} I've been mulling over your "does OsO4 oxidize prions?" question that
} you mentioned in your talk at the meeting here in Madison this week.
} Why OsO4? It's a poor protein stain, so although it's a strong
} oxidizer, it wouldn't occur to me to suspect it of degrading prions.
} Why not uranyl acetate or (better?) uranyl nitrate? These are good
} protein stains, and so should bind better to prions. Further,
} although their radioactivity is low, it is present, and as alpha
} particles. Nice and heavy, and they do lots of damage at short range.
} I should think UAc or UNO3 would be useful for breaking down prions.
} (I refuse to say "kill", as I don't think prions have any of the
} attributes of life.)
}
} Mind, I'm not going to do the experiment either. 8-)
}
} Nice talk!
}
} Phil
} --
} Philip Oshel
} Supervisor, BBPIC microscopy facility
} Department of Animal Sciences
} University of Wisconsin
} 1675 Observatory Drive
} Madison, WI 53706 - 1284
} voice: (608) 263-4162
} fax: (608) 262-5157 (dept. fax)
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Oct 18 12:30:15 2002



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.Princeton.EDU
Date: Fri, 18 Oct 2002 13:15:53 -0400
Subject: Tracking software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to hear back from those who have said they have software for tracking
cells and particle movement. Please let me know if you are ready to bring
in demo version.

Regards,

Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html



From daemon Fri Oct 18 13:52:27 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Fri, 18 Oct 2002 14:42:45 -0400
Subject: RE: WORM drives

Contents Retrieved from Microscopy Listserver Archives
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(Oh, ye who scoff at SCSI in favor of IDE)

Richard:

Easy:

Adaptec SCSI + EasySCSI + Win4.0 = it just works.

Win NT directly supports MO drives. I just went and pulled my old LF-
7303 off the shelf, checked the SCSI ID wasn't in conflict, plugged it into a
"Newly rebuilt" NT box, rebooted, and Ta-Da there it was. The SCSI interface
itself handles everything.

Panasonic actually has a BIOS update for it:

http://www.panasonic.com/support/software/pd_other.htm



http://www.panasonic.com/support/software/pd_other.htm


} If your drive is the old WORM technology, good luck finding one! I haven't seen
} one for sale in years.
}
} What computer does the 360 use? I have forgotten... Even if it is a DEC
} (perhaps especially) it would be easier to send the data to a newer PC for
} storage on hard drives and CD-Rs.
}
} Regards, Woody
}
} Woody White
} McDermott Technology Inc.
} McD: http://www.mtiresearch.com/
} Mine: http://woody.white.home.att.net
}
}
} -----Original Message-----
} } From: Richard Beanland [mailto:richard.beanland-at-bookham.com]
} Sent: Wednesday, October 16, 2002 9:12 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: WORM drives
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello everyone,
} although this is strictly not a microscopy question, I'm posting
} this in the hope that someone has been through this process already and can
} offer some advice.
}
} Our LEO 360FE SEM computer saves images on a Panasonic LF-7300 WORM drive.
} It doesn't seem feasible to connect the computer to our intranet directly
} (too old/difficult!) but a relatively straighforward way to get at the
} images is to install an identical WORM drive on a PC which is networked.
} However I imagine that some kind of software is needed to make the drive
} functional under Windows NT. My internet searches for drivers have drawn
} blanks so far. Has anyone done this and can give me some advice?
} We are asking LEO for some support on this but independent advice is
} always very valuable.
}
} Many thanks,
}
} Richard
}
}
} _______________________________
} Richard Beanland
} Analytical Services
} Bookham Technology plc
} Caswell,
} Towcester,
} Northamptonshire NN12 8EQ
} UK
} Tel: +44 (0) 1327 356362
} Fax: +44 (0) 1327 356398
} http://www.bookham.com
}
}
}
} =======================================================================
} This e-mail is intended for the person it is addressed to only. The
} information contained in it may be confidential and/or protected by
} law. If you are not the intended recipient of this message, you must
} not make any use of this information, or copy or show it to any
} person. Please contact us immediately to tell us that you have
} received this e-mail, and return the original to us. Any use,
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} No part of this message can be considered a request for goods or
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} postmaster-at-bookham.com.
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}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Fri Oct 18 14:31:44 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 18 Oct 2002 15:30:53 -0400
Subject: Foil covered SEM stubs

Contents Retrieved from Microscopy Listserver Archives
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FOLKS;

Our analytical chemist stated that aluminum foil has a coating of a
lubricant on it to facilitate separation from the roll. I was wondering if
this has caused anyone any issues with EDX.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Tina Carvalho [mailto:tina-at-pbrc.hawaii.edu]
Sent: Wednesday, October 16, 2002 1:54 PM
To: Microscopy Listserver


Hi, all-

Here is a colleague's method for using aluminum foil on SEM stubs for a
smooth background:

1. "Reynolds" seems smoothest and "regular" seems easiest to work with.

2. Burnishing. I've tried various things and they usually end up
scratching the foil. To make sure it is absolutely smooth flat, I
carefully cut a strip that is a bit wider and longer than a stub.
Place it on a clean surface, cover it with something very smooth
(weighing paper works well) and work over it with a finger tip to get
it absolutely as flat as possible. Picking it up by the very edge
with tweezers helps avoid wrinkles.

3. The smooth, flat piece is then transferred to a piece of
double-stick scotch tape and once more pressed to the tape to get a
very flat surface with no bubbles between foil and tape. This should
be done on glass or a surface from which tape plus foil can then be
carefully lifted.

4. The tape plus foil can then be applied to the stub - if it still
looks nice, proceed to step five. If wrinkles or bumps have appeared,
pull it up and try again. (You can put the tape directly on the stub
and then apply the foil - However, I have found that it works best
for me to have a nicely bonded duo of smooth tape and foil first)

5. Using weighing paper, again, press tape plus foil gently to the stub.

6. Finally, use a single-edge razor blade to trim off the excess foil
and tape around the edge of the stub. You can apply a couple of teeny
dabs of silver paste at several edge points if you want to make
absolutely certain the foil is well connected to the stub. I have
gotten into the habit of making the strip of foil plus tape slightly
narrower than stub diameter so that there is a small crescent of stub
above and below the strip, simply as a way of telling top and bottom
at a glance.

7. Additional note. For mounting an object with a convex (like my
little larval shells) or irregular lower surface, aluminum foil can
be gouged with a minuten insect pin before applying silver paste or
paint to help achieve better contact and bonding.

As with everything, it just takes a bit of experimentation. . . .


I hope this helps!

Aloha,
Tina



From daemon Fri Oct 18 14:46:58 2002



From: Mike Coviello :      coviello-at-mae.uta.edu
Date: Wed, 09 Oct 2002 14:40:39 -0700
Subject: EM-Looking for a full time electronics service engineer

Contents Retrieved from Microscopy Listserver Archives
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Hi All:
We are looking for a highly qualified, skilled individual to service
several TEM's, SEM's and other related electronic equipment across our
campus. The position is full time and permanent. The qualified
individual should be able to troubleshoot and repair many brands of
electronic equipment down to the component level.
If interested, please forward a resume to me.
Thanks,
Michael Coviello
Lab Manager,
The University of Texas at Arlington,
Arlington, TX



From daemon Fri Oct 18 16:20:26 2002



From: Lawrence Mason :      LMason-at-wyeth.com
Date: Fri, 18 Oct 2002 17:05:36 -0400
Subject: unsubscribe

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unsubscribe



From daemon Fri Oct 18 17:06:32 2002



From: JoAn Hudson :      hudson-at-uoneuro.uoregon.edu
Date: Fri, 18 Oct 2002 15:02:32 -0700
Subject: Looking for book reviewers

Contents Retrieved from Microscopy Listserver Archives
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I have 4 books ready for review. If you would like to review any of
these titles and write your review for the journal please let me know
ASAP. Reviewers will be chosen on a first come first serve basis based
on your interests. I would also like to know if you would review other
books as they come available if your selection has already been sent
out.

List:

1) Electron Backscatter Diffraction in Materials Science, Edited by
Adam J. Schwartz, Mukul Kumar, and Brent L. Adams

2) Scanning Tunneling Microscopy and its Applications, By C.Bai

3) PCR/RT-PCR in situ, light and electron microscopy, By Gerard
Morel and Mireille Raccurt

4) Quick Photoshop for research, a guide to digital imaging for
photoshop 4x,5x,6x,7x, by Jerry Sedgewick

If interested please e-mail me along with a mailing address for you.
Thank you very much!
JoAn

JoAn Hudson, PhD.
Institute of Neuroscience
222 Huestis Hall
University of Oregon,
Eugene, OR 97403
 
Telephone: (541) 346 4508
FAX: (541) 346 4548






From daemon Fri Oct 18 18:01:55 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Fri, 18 Oct 2002 15:53:45 -0700
Subject: Electropolishing Solution required

Contents Retrieved from Microscopy Listserver Archives
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Dear Fred:

I will apologize in advance in that my recipe is for our Model 550D
Single Vertical Jet ElectroPolisher rather than the other systems you
mentioned. Seems like there is always a solution with one of our
products! ;-)

In any case, I do have a paper from Bernie Kestel titled:

"Jet Thinning of YBa2Cu3Ox High Tc Superconductor and Gold for TEM with
a Non-Acid Electrolyte"

This is Technical Report #14 on our online list. Unfortunately, it is
not in electronic format, but the reference is Ultramicroscopy 25 (1988)
351-354. I can also send you a photocopy of the paper if that would be
easier for you.

While some of our recipes appear to be interchangeable with the twin jet
systems. I believe that his BK-2 solution (non-acid electrolyte) is too
viscous to be pumped through the twin jet units. However, it may give
you a good starting point.

DISCLAIMER: South Bay Technology, Inc. produces equipment and supplies
as described above and, therefore, has a vested interest in promoting
their use.

Best regards-

David

Fred Pearson wrote:


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Dear Group:

I have the task of preparing TEM foils from 1 mm. O.D. 18K and 14K
Gold
wire. I was successful in ion milling dimpled 50um section/foils in
PIPS
at 4Kv., 4 degree angle. We are looking at the strain fields about
100nm.
precipitates. The ion milling caused damaged to the structure as
indicated
by the ripple patterns in the material as viewed in the TEM. Obviously

this may/can alter our examination.

Next, I would like to try electropolishing the material if I can make
suitable foils for the polisher. We cold rolled the wire, and punched
out
3 mm. disks to work with. Electropolishing is the next step to try.

What I am asking, does anyone know of an electropolishing solution for
Au,
suitable for a Tenupol 5 or a Fischione machine?

Thanks in advance

Fred Pearson

********************************************************
Fred Pearson
Brockhouse Institute for Materials Research
McMaster University
1280 Main St. West
Hamilton, Ontario
Canada L8S 4M1

email: eoptics-at-mcmaster.ca
phone: (905) 525-9140 ext. 24609
fax: (905) 521-2773
********************************************************

--
David Henriks
Vice President

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

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Metallogaphy, Crystallography and Electron Microscopy.

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calling +1-949-492-2600 and
delete this message from your system.




From daemon Fri Oct 18 21:14:04 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Fri, 18 Oct 2002 22:01:04 -0500
Subject: Thanks for the Stereo/Compound LM replies

Contents Retrieved from Microscopy Listserver Archives
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Thanks to eveyone who vetured to reply to my only half tongue in
cheek posting about which microscopes are really compound. I
learned that some stereomicroscopes have only one objective lens
(!?) which must call some interesting optical designs.




From daemon Fri Oct 18 21:28:51 2002



From: Greg Barclay :      gbarclay-at-trinidad.net
Date: Fri, 18 Oct 2002 22:18:02 -0500
Subject: Tissue shrinkage in Tousimis CPD

Contents Retrieved from Microscopy Listserver Archives
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I am seeking advice on the correct way to operate a Tousimis semiautomatic CPD to minimize if not eliminate
plant tissue shrinkage.

I would appreciate any suggestions.

Thank you.

Greg Barclay




From daemon Sat Oct 19 06:44:48 2002



From: dieter-at-magnet.at (Dieter Aigner)
Date: Sat, 19 Oct 2002 13:30:05 +0200
Subject: Need help with LEITZ 1512 microtome

Contents Retrieved from Microscopy Listserver Archives
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Dear Sirs,

I have received an used Leitz 1512 microtome a few days ago and are not
very
experienced with it. Everything works perfectly but I can not manage it to
set the
THICKNESS to MORE THAN 11 my. I can rotate the knob only from 0-11.
Is the microtome broken?
I do not have a manual for the microtome.

Please can anyone help me?

Many thanks.

Dieter, AUSTRIA




From daemon Sun Oct 20 14:46:32 2002



From: Diane G. Miller :      millerd-at-coho.net (by way of MicroscopyListserver)
Date: Sun, 20 Oct 2002 14:13:45 -0500
Subject: Convert bitmap to tiff or jpeg

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

I was wondering if some of you could forward me some sites for converting
bitmap images to tiff or jpeg. I would appreciate it very much.

Thanks for your help.
Diane

Diane G. Miller
Miller Consultant Service
503-784-6444
millerd-at-coho.net


From daemon Sun Oct 20 20:05:38 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Sun, 20 Oct 2002 19:55:16 -0500
Subject: Re: Tissue shrinkage in Tousimis CPD

Contents Retrieved from Microscopy Listserver Archives
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Greg,

This depends a great deal on the nature and size of the plant
tissues. We do a lot of small, herbaceous specimens here, mostly
Arabidopsis, but I've done other plant tissues as well, such as roots.
The trick is to ignore the directions in the Tousimis manual -- for
any biological specimens. What you need to do instead is to let the
specimens soak in the liquid CO2 for a given amount of time after
first purging the chamber of the absEtOH that was in it when the
samples were loaded. Then purge the chamber to replace the lq CO2
with fresh lq CO2, soak, and repeat N times. This is analogous to the
dehydration process where the water in the tissues is replaced with
EtOH. Now, the EtOH in the tissues is being replaced with lq CO2.
Once that is done, then and only then can the samples be dried.
Otherwise, they are being dried from EtOH.
The length of the soaks and number of soaks is rather empirical and
highly dependent on tissue type and size. For the small (a few mm),
herbaceous Arabidopsis specimens, we generally use 4 soaks for 10
minutes each. For 1 cm^2 bone, I've used 5 soaks of 30 - 60 minutes
each. For fetal rat urogential sinus, 3 soaks of 2 to 3 minutes each
works. For small (meaning a cm to mm) crustaceans and their
appendages, which have relatively impermeable cuticles, I used 5
soaks of 5 or 10 minutes each.
There can be some extraction of materials by the lq CO2 and possibly
some artifacts from too long in lq CO2, but it is a much worse sin to
run the specimens through CO2's critical point while they still
retain EtOH. It is imperative to make certain that *all* of the EtOH
has been replaced by lq CO2.
This means you'll have to try different times and number of soaks.
The larger and denser or woodier your samples, the more soaks for
longer times you'll need.
Some of the times I've given could perhaps be shortened. Given that
too long likely won't produce significant artifact (note the
"likely") and will only take more time, but that too little time will
certainly produce ruined specimens which wastes a *lot* more time,
specimens, and more, I'd rather spend the (possibly) extra time on
the EtOH/lq CO2 exchanges.

Phil

} I am seeking advice on the correct way to operate a Tousimis
} semiautomatic CPD to minimize if not eliminate
} plant tissue shrinkage.
}
} I would appreciate any suggestions.
}
} Thank you.
}
} Greg Barclay

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Mon Oct 21 00:03:59 2002



From: Thomas Litzinger :      thomas_c_litzinger-at-yahoo.com
Date: Sun, 20 Oct 2002 21:54:08 -0700 (PDT)
Subject: Re: Tissue shrinkage in Tousimis CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe


__________________________________________________
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Y! Web Hosting - Let the expert host your web site
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From daemon Mon Oct 21 04:57:00 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 21 Oct 2002 10:46:55 +0100 (GMT Daylight Time)
Subject: Re: Tissue shrinkage in Tousimis CPD

Contents Retrieved from Microscopy Listserver Archives
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You said re CPD:

"There can be some extraction of materials by the lq CO2
and possibly some artifacts from too long in lq CO2...."

We are doing a student project here into SEM preparation
methods for pollen. I wonder if you could let me know if
you have any references for the above comment?

Thanks

Dave


On Sun, 20 Oct 2002 19:55:16 -0500 Philip Oshel
{peoshel-at-facstaff.wisc.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greg,
}
} This depends a great deal on the nature and size of the plant
} tissues. We do a lot of small, herbaceous specimens here, mostly
} Arabidopsis, but I've done other plant tissues as well, such as roots.
} The trick is to ignore the directions in the Tousimis manual -- for
} any biological specimens. What you need to do instead is to let the
} specimens soak in the liquid CO2 for a given amount of time after
} first purging the chamber of the absEtOH that was in it when the
} samples were loaded. Then purge the chamber to replace the lq CO2
} with fresh lq CO2, soak, and repeat N times. This is analogous to the
} dehydration process where the water in the tissues is replaced with
} EtOH. Now, the EtOH in the tissues is being replaced with lq CO2.
} Once that is done, then and only then can the samples be dried.
} Otherwise, they are being dried from EtOH.
} The length of the soaks and number of soaks is rather empirical and
} highly dependent on tissue type and size. For the small (a few mm),
} herbaceous Arabidopsis specimens, we generally use 4 soaks for 10
} minutes each. For 1 cm^2 bone, I've used 5 soaks of 30 - 60 minutes
} each. For fetal rat urogential sinus, 3 soaks of 2 to 3 minutes each
} works. For small (meaning a cm to mm) crustaceans and their
} appendages, which have relatively impermeable cuticles, I used 5
} soaks of 5 or 10 minutes each.
} There can be some extraction of materials by the lq CO2 and possibly
} some artifacts from too long in lq CO2, but it is a much worse sin to
} run the specimens through CO2's critical point while they still
} retain EtOH. It is imperative to make certain that *all* of the EtOH
} has been replaced by lq CO2.
} This means you'll have to try different times and number of soaks.
} The larger and denser or woodier your samples, the more soaks for
} longer times you'll need.
} Some of the times I've given could perhaps be shortened. Given that
} too long likely won't produce significant artifact (note the
} "likely") and will only take more time, but that too little time will
} certainly produce ruined specimens which wastes a *lot* more time,
} specimens, and more, I'd rather spend the (possibly) extra time on
} the EtOH/lq CO2 exchanges.
}
} Phil
}
} } I am seeking advice on the correct way to operate a Tousimis
} } semiautomatic CPD to minimize if not eliminate
} } plant tissue shrinkage.
} }
} } I would appreciate any suggestions.
} }
} } Thank you.
} }
} } Greg Barclay
}
} --
} }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
} Philip Oshel
} Supervisor, AMFSC and BBPIC microscopy facilities
} Department of Animal Sciences
} University of Wisconsin
} 1675 Observatory Drive
} Madison, WI 53706 - 1284
} voice: (608) 263-4162
} fax: (608) 262-5157 (dept. fax)
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Oct 21 05:14:37 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Mon, 21 Oct 2002 11:06:30 +0100
Subject: WORM drives - thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had several very useful replies, thankyou to everyone who took the time to
help.
Although I am now able to connect and have the drive working on a WinNT
PC, it appears that the disks are unreadable. The LEO 'scope has an
operating system which pre-dates Win95 (even Win3.1, I think!), and the file
allocation tables used on the MO disk can't be deciphered by the modern PC.
So, it looks like I'll have to explore other routes. At the moment this
looks like getting a CD-RW drive (if possible, I suspect there will be
problems along this route too). I feel it should be possible to put a cable
between adjacent boxes of electronics and send signals from one to another
without the need for a physical medium inbetween, but maybe not...

Thanks again,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com



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From daemon Mon Oct 21 07:12:45 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Mon, 21 Oct 2002 06:59:05 -0500
Subject: RE: Convert bitmap to tiff or jpeg

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Most image editors will let you save images in a multitude of formats. I
your case it would be load-resave steps. Some may allow batch processing.

Examples:
IrView http://www.irfanview.com/
Or VuePrint http://www.hamrick.com/

Regards, Woody




Woody White
McDermott Technology Inc.
McD: http://www.mtiresearch.com/
Mine: http://woody.white.home.att.net






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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello All,

I was wondering if some of you could forward me some sites for converting
bitmap images to tiff or jpeg. I would appreciate it very much.

Thanks for your help.
Diane

Diane G. Miller
Miller Consultant Service
503-784-6444
millerd-at-coho.net


From daemon Mon Oct 21 08:49:44 2002



From: Gilles Hug :      gilles.hug-at-onera.fr
Date: Mon, 21 Oct 2002 15:40:11 +0200
Subject: Re: Antivibration platform

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Le jeudi, 17 oct 2002, à 18:10 Europe/Paris, Dusevich, Vladimir a écrit
:

}
} We are going to move our EM lab to a new place
} (in the same building) and are planning to put an
} anti-vibration platform under the column of our
} FEG-ESEM XL30. Do we have to place a HV transformer
} on the same platform (the transformer is right behind
} the column)?

Yes if the transformer is linked to the microscope with a rigid cable
it would be betterto have it on the platform. Unless it generates by
itself mechanical vibrations.

}
} Any additional advise will be very helpful also,
} especially experience with anti-vibration platforms
} of different manufacturers.

We have recently built a platform for a new microscope. It is composed
of a concrete block. I am sending you also pdfs of recent measurements.
As you can see on the graphs we are rather satisfied of the results.
The filtering system consists of a concrete bloc of 15 Mkg (tons)
suspended with 4 springs with dampers at each corners. The company Gerb
delivered the system. They made a calculation that the cutoff frequency
would be around 2-3 Hz. Generally speaking such arrangement can be view
as to spring in series (on for the block and one for the microscope).
You must take care that there is no resonance between the to
oscillators. In my case I made a quick estimate, which showed that this
is not a problem. In fact as a rule of thumb the weight of the
microscope should be negligible as compared to the platform. So design
you platform with 10 times the weight of the microscope.
The shape is also important. The vertical motion is normally the more
important mode. But, if for example the shape of the concrete block is
tall rather than flat, you may be annoyed by rotations around X, Y
axis. This might excite flexion modes of the column, especially if
their is some attachments like X-ray detector..
In our case we also have a preexisting concrete floor that is probably
very thick but unfortunately connected to the building (old building).
This probably why we can see the vibrations coming from the air
conditioning which is located on the roof. If you make a new building
you should consider building a concrete floor *de-connected* to the
building and the your platform on dampers.
Also, I have to mention that the operator is NOT on the platform but on
a wooden floor above it.
Finally, last advice and not the least: check EVERYTHING YOURSELF.
Generally, the workers don't understand why you ask specific things and
if they don't take care they can ruin your beautiful design. We had a
mason who accidentally poured liquid concrete that you use to flatten
the floors. Some of it went below the platform and re-connected it with
the building!!! Fortunately if was not very difficult to remove. But
what would happen if we haven't noticed?

Note about the graphs: please note that the vertical scales are
correct. These measurements have been performed by our "vibration”
department at Onera. Usually , they study vibrations modes of Aibuses
and other flying machines. They had to build a special low noise
amplifier for those measurements. This is the reason why we can see
these very small contributions. I guess that with commercial equipments
you would not be able to see these peaks above the noise.
Best,
Gilles

PDFs : ask me if interested by the figures


From daemon Mon Oct 21 09:57:36 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Mon, 21 Oct 2002 10:47:01 -0400
Subject: Renal EM Job

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers:

Our renal pathologist is moving to Columbus, Ohio and is taking a position
at Ohio State. He is looking for a trained EM tech who is experienced in
renal pathology EM. Contact me off list directly to my email address if
anyone is interested.

karen_bentley-at-urmc.rochester.edu

Karen


Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954



From daemon Mon Oct 21 10:00:55 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Mon, 21 Oct 2002 16:54:03 +0200
Subject: Alternatives to C films

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
What are the alternatives to C-support films, for supporting powder
particles. We have a situation where we want to determine the C content
of an inorganic phase and don't want a C film, which would mess up the
quantification.

Thanks
--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Mon Oct 21 10:10:28 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 21 Oct 2002 10:03:37 -0500
Subject: Re: WORM drives - thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello again. Sorry to hear about the difficulties.

The first order of business will be to find out what system is running the
LEO. After that is done, you can probably explore options. They may not all
be that much fun. There is a possibility that there will not only be an
operating system incompatibility, but also a file format compatibility.

We were running a Kevex EDS system when the Internet started coming on big.
We much wanted to make files available over the net - me especially because
I had my office in another building. The Kevex operated around a DEC PDP-11
computer with the TSX+ operating system. (Those aren't exactly common.) The
first order of business was to connect that to the ethernet. We were able
to do it but it required a special card costing several hundred dollars
(now you can buy $10 ethernet cards for PCs) and TCP-IP software costing
many hundreds more (compared to the built in support in modern OSs). Then
we had to convert the proprietary Kevex file format over to a portable
format (TIF). Once we had crossed those two not-so-small hurdles, we were
up and running just fine.

The other approach, like you have been trying, was to find a common media
format so that we could transfer removable media between two computers.
Someone had written a utility to read PDP-11 disks on a PC (getting around
the different allocation and directory structures), but I think it may have
been limited to floppy disks. We were using 10 MB Bernoulli cartridges from
Iomega. I vaguely recall that there were other issues trying to access
those disks. Even if we had succeeded, there would still have been the
issue of converting the file formats.

I would like to think that it will not be so bad for you. I would like to
think that the LEO is built around a PC. But LEO or another LEO user would
probably have to verify that. They should also be able to tell which OS and
what disk system are being used. Then you could move on from there.

Warren

At 11:06 AM 10/21/02 +0100, you wrote:

} I had several very useful replies, thankyou to everyone who took the time to
} help.
} Although I am now able to connect and have the drive working on a WinNT
} PC, it appears that the disks are unreadable. The LEO 'scope has an
} operating system which pre-dates Win95 (even Win3.1, I think!), and the file
} allocation tables used on the MO disk can't be deciphered by the modern PC.
} So, it looks like I'll have to explore other routes. At the moment this
} looks like getting a CD-RW drive (if possible, I suspect there will be
} problems along this route too). I feel it should be possible to put a cable
} between adjacent boxes of electronics and send signals from one to another
} without the need for a physical medium inbetween, but maybe not...
}
} Thanks again,
}
} Richard

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Mon Oct 21 10:26:56 2002



From: Dmitry Lobanov :      dima-at-ipm.sci-nnov.ru (by way of
Date: Mon, 21 Oct 2002 10:06:30 -0500
Subject: TEM Need help on Ion Etching Device

Contents Retrieved from Microscopy Listserver Archives
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Now I am looking for an electrical motors

5116-207GE M:1516 E 0065 + G:15/3 (reduction ratio 900:1)
and
5116-211GE M:1516 E 0065 + G:15/3 (reduction ratio 1670:1)

They are need for positioning and rotation of specimen table in
BALZERS Universal Ion Beam Etching Device IEU 100.

With kind regards,
Dmitri Lobanov.


Institute for physics of microstructures RAS
603950, N.Novgorod, GSP-105, Russia
phone: +7(8 312)675037
fax: +7(8 312)609111
E-mail: dima-at-ipm.sci-nnov.ru


From daemon Mon Oct 21 10:30:36 2002



From: David_Bell-at-millipore.com
Date: Mon, 21 Oct 2002 11:24:08 -0400
Subject: Tensile Stage in ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello fellow microscopists,

I am looking for a lab that has a tensile stage mounted in an ESEM that is
willing to run a couple of samples. I would prefer a service lab, however
if there is a commercial or university lab that is willing to take on the
task, that would be fine as well. The samples would be polymer films and
I would be interested in images corresponding to the various force data.
If you have this capability, please contact me off list.

Thanks in advance,



David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108
David_Bell-at-Millipore.com


From daemon Mon Oct 21 10:54:40 2002



From: Berg, R. Howard :      RHBerg-at-danforthcenter.org
Date: Mon, 21 Oct 2002 10:48:56 -0500
Subject: variable temperature hot plate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

Does anyone know a source for a variable temperature hot plate? We
want to use it for wax embedding, for pouring in situ boats, allowing
us to slide em over and readjust the tissue as it solidifies.


Howard


From daemon Mon Oct 21 10:58:47 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Mon, 21 Oct 2002 11:51:44 -0400
Subject: Diagnostic EM Job

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

Is anyone interested in a diagnostic EM job? You must have experience in
renal pathology and tumors. Our renal pathologist is moving to Ohio and is
looking for anyone in the USA who is interested and available.

Please contact me off list at: karen_bentley-at-urmc.rochester.edu

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954



From daemon Mon Oct 21 13:19:11 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 21 Oct 2002 14:09:49 -0400
Subject: RE: Convert bitmap to tiff or jpeg

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My favorite is ThumbsPlus
http://www.cerious.com/

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: White, Woody N. [mailto:nwwhite-at-mcdermott.com]
Sent: Monday, October 21, 2002 7:59 AM
To: 'Diane G. Miller'; microscopy-at-sparc5.microscopy.com


Most image editors will let you save images in a multitude of formats. I
your case it would be load-resave steps. Some may allow batch processing.

Examples:
IrView http://www.irfanview.com/
Or VuePrint http://www.hamrick.com/

Regards, Woody




Woody White
McDermott Technology Inc.
McD: http://www.mtiresearch.com/
Mine: http://woody.white.home.att.net






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello All,

I was wondering if some of you could forward me some sites for converting
bitmap images to tiff or jpeg. I would appreciate it very much.

Thanks for your help.
Diane

Diane G. Miller
Miller Consultant Service
503-784-6444
millerd-at-coho.net


From daemon Mon Oct 21 13:36:54 2002



From: Shu-You Li :      syli-at-northwestern.edu
Date: Mon, 21 Oct 2002 13:28:11 -0500
Subject: Immediate Postdoc position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I am posting this message for Prof. Dravid. Please contact him directly.
Thank you,
Shuyou.
=======================================================
IMMEDIATE POSTDOCTORAL POSITION AVAILABLE:

CRYO-ANALYTICAL TRANSMISSION ELECTRON MICROSCOPY
OF SOFT AND HYBRID NANOSTRUCTURES

INSTITUTE FOR ENVIRONMENTAL CATALYSIS, NORTHWESTERN UNIVERSITY

Applications are invited for a full-time postdoctoral research associate within the Institute for Environmental Catalysis (IEC) at Northwestern University. The position is jointly supervised by Profs. Vinayak P. Dravid of Materials Science & Engineering, and Prof. Jean-Francois Gaillard of Civil Engineering, at Northwestern University.

We seek a dynamic and energetic individual with prior experience in advanced electron microscopy of micobiological, geochemical, or materials systems to conduct frontier research using state-of-the-art TEM instrumentation; and design novel approaches to observe aquatic environmental particles in-situ in TEM. Hands-on experience in preparatory methods such as cryo-preparation, ultramicrotomy etc. will be a strong asset, as well as knowledge of STEM-EDS, PEELS, and energy filtering.

Applications should include a vita, a brief statement of research interests, and names of at least three references including email addresses and telephone numbers.

The position is open only to those eligible for employment in the US.

Applications in PDF format should be sent by email to: v-dravid-at-northwestern.edu

Professor Vinayak P. Dravid,
Materials Science & Engineering, and
Institute for Environmental Catalysis
2225 N. Campus Drive, 1133 Cook Hall (formerly MLSF)
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 467-6573
E-mail: v-dravid-at-northwestern.edu
http://vpd.ms.northwestern.edu
http://epic.ms.northwestern.edu

_____________________________
Shu-You Li, Ph.D.
Electron Microscopist
Electron Probe Instrumentation Center(EPIC)
Northwestern University
2220 Campus Drive, 1141 Cook Hall
Evanston, IL 60208, USA
Ph: (847) 491-7798, Fax: (847) 491-7820
Email: syli-at-northwestern.edu; syli16-at-hotmail.com
http://pubweb.northwestern.edu/~sli974





From daemon Mon Oct 21 14:05:49 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 21 Oct 2002 14:57:44 -0500
Subject: Non-carbon-containing TEM support films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ian MacLaren wrote:
=========================================================
What are the alternatives to C-support films, for supporting powder
particles. We have a situation where we want to determine the C content of
an inorganic phase and don't want a C film, which would mess up the
quantification.
==========================================================
There are two possibilities:

a) silicon dioxide/monoxide films made via the evaporation of SiO, see URL
http://www.2spi.com/catalog/spec_prep/evapor_3b.shtml or the completed
films can be purchased already made from SPI Supplies (and some of our
competitors), see URL
http://www.2spi.com/catalog/grids/cusctgrd.html

or

b) silicon nitride membrane window TEM grids as shown on URL
http://www.2spi.com/catalog/instruments/silicon-nitride.shtml

Either way, you will have a good support that will be completely free of any
carbon.

Disclaimer: SPI Supplies provides all three items mentioned, namely the SiO
chips, already custom coated SiO2 TEM grids, and the silicon nitride
membrane window grids.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Mon Oct 21 14:17:07 2002



From: Becky Holdford :      r-holdford-at-ti.com
Date: Mon, 21 Oct 2002 14:10:04 -0500
Subject: contract micro-FTIR work in Texas area?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If anyone has any information about labs that do contract
micro-FTIR work in the Texas area, please forward it to me.
We are looking for a source for this type of work closer
than Sunnyvale, CA.

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Mon Oct 21 14:35:51 2002



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Mon, 21 Oct 2002 15:23:04 -0400
Subject: TEM Specimen Preparation Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A TEM Specimen Preparation Course Will be offered March 12-14, 2003 at the
Materials Characterization Facility at the University of Central Florida,
Orlando, FL USA

A review of all techniques will be given, with primary emphasis on tripod
polishing, ion milling, and all latest FIB techniques

Instructors:
Ron Anderson, Microscopy Today
Fred Stevie, NC State
Lucille Giannuzzi, UCF
Brian Kempshall, UCF

For more information contact Lucille Giannuzzi, lag-at-mail.ucf.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Mon Oct 21 14:35:53 2002



From: pkrsko-at-stevens-tech.edu (by way of MicroscopyListserver)
Date: Mon, 21 Oct 2002 14:24:37 -0500
Subject: Ask-A-Microscopist:Calibration Standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (pkrsko-at-stevens-tech.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
October 21, 2002 at 13:31:19
---------------------------------------------------------------------------

Email: pkrsko-at-stevens-tech.edu
Name: Peter Krsko

Organization: Stevens Institute of Technology

Education: Graduate College

Location: Hoboken, NJ 07030

Question: Dear Sirs,

Where can I purchase some kind of calibration sample for fluorescent
imaging? I would like to qauntify my results, but I am not sure what
intensity corresponds to what concentration of FITC. Are there also
calibration samples for reflective fluoro microscopy?

Thank you very much

---------------------------------------------------------------------------


From daemon Mon Oct 21 14:39:51 2002



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Mon, 21 Oct 2002 15:31:43 -0400
Subject: FIB FIG CALL FOR PAPERS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


CALL FOR PAPERS FIB Focused Interest Group (FIG) WORKSHOP

Tuesday, March 18, 2003

Student Union, University of Central Florida, Orlando, FL 32816

(in conjunction with a TEM Specimen Prep Course March 12-14, 2003, and the
FL AVS/Florida Society for Microscopy Meeting, March 17-18, 2003).

Please submit an abstract (limit 250 words), including authors and
affiliations, by email to:

Dr. Lucille Giannuzzi
lag-at-mail.ucf.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Mon Oct 21 16:04:30 2002



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Mon, 21 Oct 2002 16:48:30 -0600
Subject: Muscle TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

I would appreciate recieveing any fixation protocol you know
works well for mouse muscle (extensor digitorum longus (EDL)
and epitrochlearus (EPI)). We use routinely immersion into
2.5% glutaraldehyde in 0.1M cacodylate pH 7.4, but have had
often problems with mitochondrial swelling. Since this project is
to quantify mitochondrial density, we do need perfect and
reliable fixation of all mitos. Perfusion is not an option. Thanks
for any advise on type of fixative, buffer and temperature.

Marc
--
Marc Pypaert
Department of Cell Biology,
Center for Cell and Molecular Imaging and
Ludwig Institute for Cancer Research,
Yale University School of Medicine
333 Cedar Street
New Haven CT 06520
Tel : (203) 785 3681
Fax : (203) 785 7446




From daemon Mon Oct 21 17:21:48 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 21 Oct 2002 18:09:25 -0400
Subject: Need a high temperature epoxy for microtoming

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Why don't you embed the samples in glass? You are at the softening temperatures of glass. We have a technique here that we bond two pieces of glass together by heating them in a plasma etching system. This technique was originally developed and published by Prof Carlos Pantano at Penn State University to profile Sn on the bottom surface of glass. What I would do is sprinkle some of your dust on one of the glass surfaces, bring the two glass surfaces together and heat them up. Then dimple and ion mill normally. I have no idea whether you particles will wet the glass or not, but they might.

Another option is to use a ceramic bonding adhesive in a similar manner to above. There is a description of a high temperature XTEM prep in the MRS TEM sample Prep book number 1 on what to use. The adhesive was from Aremco. Take two piece of Si and put your adhesive on one surface, dust the adhesive and make your sandwich. Alternatively, you could just embed them in the ceramic adhesive and prepare the disk as a plan view sample.

If you choose one of these ideas, I would love to know how it works out.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."




-----Original Message-----
} From: "George_Munzing-at-engelhard.com"-at-sparc5.microscopy.com
[mailto:"George_Munzing-at-engelhard.com"-at-sparc5.microscopy.com]
Sent: Tuesday, October 15, 2002 9:23 AM
To: Microscopy-at-sparc5.microscopy.com


Hello fellow microscopists,

I need to do some high temperature TEM studies and am looking for a
suitable mounting resin. I will be embedding and microtoming powder
materials and would like to go as high in temperature as possible. The
materials themselves can withstand the temperatures but I doubt my
conventional epoxy would survive.

Is there any silicone epoxy or other suitable material designed to
withstand high temperatures (maybe as high as 600-800C) for a short time
for embedding?

Any suggestions you can offer are greatly appreciated.

Thanks in advance!

George R. Munzing Jr.
Engelhard Corporation
Strategic Technologies Group
25 Middlesex/Essex Turnpike
Iselin, NJ 08830
Tel: 732-205-7030
FAX: 732-205-5300



From daemon Mon Oct 21 17:43:09 2002



From: Arrowood, Roy :      arrowood-at-utep.edu
Date: Mon, 21 Oct 2002 16:36:10 -0600
Subject: variable hot plate source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Howard,

Cole-Parmer lists temperature-controlled hot plates (made by Heisen, and
probably others)--from fixed setpoint units with +/-1 degree temperature
control to fancy programmables. I'm pretty sure that the other major lab
supply houses (Fisher, VWR, etc.) also offer variable-temperature hot
plates, but I don't have catalogs handy at the moment, so I can't verify
that.

You might also consider using a Variac(TM) (or other variable
autotransformer) to power a basic, old-fashioned clunker of a hot plate. If
you use that approach, make sure the power capacity of the autotransfomer
matches the power of the hot plate. Don't buy a Variac that would let users
dial in too high a voltage, which could fry the hot plate and/or present
safety hazards. And don't use the transformer approach if your hot plate
has fancy extras such as stirmotors, digital displays, etc.---many devices
other than plain-vanilla electrical resistance heaters can be damaged by
undervoltage. Also, the autotransformer strategy would require that you
invest some time to "calibrate" your setup: i.e,, to find out at what
voltage the hot plate maintains a satisfactory and safe temperature for
what you want to do. Changing the environment or setup (anything that
increases or decreases heat losses from the hot plate) would Furthermore,
autotransformers are pricey items (as are T-controlled hot plates).

So your best bet, probably, is to buy a nice temperature-controlled hot
plate, as you indicated.

Hope this helps.

-------Roy

P.S. I have no connection to any of the suppliers or manufacturers
mentioned, and no preference for any supplier is intended. Shop with care
to make sure anything you buy meets your requirements.


======================
Roy Arrowood arrowood-at-utep.edu
Metallurgical and Materials Engineering
University of Texas at El Paso
El Paso, TX 79968-0520
(915)747-6934 voice // (915)747-8036 FAX
(915)747-5468 secretary



From daemon Mon Oct 21 23:39:20 2002



From: Tim Richardson :      mirlyn-at-attglobal.net
Date: Mon, 21 Oct 2002 18:40:15 -0600
Subject: Synchronization of cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a method or protocol for synchronizing cells in tissue
culture?

We are looking to find a method so that all cells in the culture are at
substantially the same point in their cell cycle? It is also important
that the cells are still alive and living as normally as cells do when in
tissue culture.

We are most interested in achieving this goal with human fibroblasts in
culture, but would be interested in any success with other cells lines of
any origin, mammal, animal, or plant.

Thank you for your assistance, any information on achieving this goal will
be helpful.

Tim Richardson,
RTI,
Phone: 905-951-7058
Fax: 905-951-7052

email: mirlyn-at-attglobal.net





From daemon Tue Oct 22 02:31:48 2002



From: William.Ho-at-glencore.com.sg
Date: Tue, 22 Oct 2002 15:15:01 +0800
Subject: pls take me off the e-mail list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


i have no idea why i'm on it in the first place



From daemon Tue Oct 22 06:51:25 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 21 Oct 2002 10:46:55 +0100 (GMT Daylight Time)
Subject: Re: Tissue shrinkage in Tousimis CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You said re CPD:

"There can be some extraction of materials by the lq CO2
and possibly some artifacts from too long in lq CO2...."

We are doing a student project here into SEM preparation
methods for pollen. I wonder if you could let me know if
you have any references for the above comment?

Thanks

Dave


On Sun, 20 Oct 2002 19:55:16 -0500 Philip Oshel
{peoshel-at-facstaff.wisc.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greg,
}
} This depends a great deal on the nature and size of the plant
} tissues. We do a lot of small, herbaceous specimens here, mostly
} Arabidopsis, but I've done other plant tissues as well, such as roots.
} The trick is to ignore the directions in the Tousimis manual -- for
} any biological specimens. What you need to do instead is to let the
} specimens soak in the liquid CO2 for a given amount of time after
} first purging the chamber of the absEtOH that was in it when the
} samples were loaded. Then purge the chamber to replace the lq CO2
} with fresh lq CO2, soak, and repeat N times. This is analogous to the
} dehydration process where the water in the tissues is replaced with
} EtOH. Now, the EtOH in the tissues is being replaced with lq CO2.
} Once that is done, then and only then can the samples be dried.
} Otherwise, they are being dried from EtOH.
} The length of the soaks and number of soaks is rather empirical and
} highly dependent on tissue type and size. For the small (a few mm),
} herbaceous Arabidopsis specimens, we generally use 4 soaks for 10
} minutes each. For 1 cm^2 bone, I've used 5 soaks of 30 - 60 minutes
} each. For fetal rat urogential sinus, 3 soaks of 2 to 3 minutes each
} works. For small (meaning a cm to mm) crustaceans and their
} appendages, which have relatively impermeable cuticles, I used 5
} soaks of 5 or 10 minutes each.
} There can be some extraction of materials by the lq CO2 and possibly
} some artifacts from too long in lq CO2, but it is a much worse sin to
} run the specimens through CO2's critical point while they still
} retain EtOH. It is imperative to make certain that *all* of the EtOH
} has been replaced by lq CO2.
} This means you'll have to try different times and number of soaks.
} The larger and denser or woodier your samples, the more soaks for
} longer times you'll need.
} Some of the times I've given could perhaps be shortened. Given that
} too long likely won't produce significant artifact (note the
} "likely") and will only take more time, but that too little time will
} certainly produce ruined specimens which wastes a *lot* more time,
} specimens, and more, I'd rather spend the (possibly) extra time on
} the EtOH/lq CO2 exchanges.
}
} Phil
}
} } I am seeking advice on the correct way to operate a Tousimis
} } semiautomatic CPD to minimize if not eliminate
} } plant tissue shrinkage.
} }
} } I would appreciate any suggestions.
} }
} } Thank you.
} }
} } Greg Barclay
}
} --
} }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
} Philip Oshel
} Supervisor, AMFSC and BBPIC microscopy facilities
} Department of Animal Sciences
} University of Wisconsin
} 1675 Observatory Drive
} Madison, WI 53706 - 1284
} voice: (608) 263-4162
} fax: (608) 262-5157 (dept. fax)
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Oct 22 07:15:01 2002



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Tue, 22 Oct 2002 08:07:19 -0400
Subject: Re: Muscle TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Marc,
We use to routinely fix mouse muscle by immersion but used 3.5% glut. in
.1M cacodylate at 4 degrees for 1.5 hours. The pieces need to be very
small. Add 4% sucrose to the buffer washes. The pH should be 7.4. Osmium
should be 1% in the same buffer at 4 degrees also.
Good luck,
Mary Gail

At 04:48 PM 10/21/02 -0600, Marc Pypaert wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-9700


From daemon Tue Oct 22 09:07:50 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 22 Oct 2002 09:48:46 -0400
Subject: Re: Muscle TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Marc,
I've always had goo results in muscle (skeletal and cardiac) with
the following fix:
4% pfa (methanol-free, diluted from 16% stock )
2.5% glutaraldehyde
in 0.1M Na-cacodylate buffer
My "recipe", making this up from 10ml stock vials of 16% pfa and 10%
glut yields 40 ml of fix, to which I then add 2 ml of saturated
picric acid (aqueous). (based on Ito & karnovsky, J Cell Bio,
abstract 168a, 1968)

Try to hold the muscles in an extended state, either by leaving them
attached to the leg bone, or using ligature to tie them to small
wooden sticks. This will help prevent overly contracted sarcomeres
and give a more "textbook" appearance with M-lines, A & I bands, etc.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Oct 22 09:10:52 2002



From: David BG Rose :      drose-at-wlgore.com
Date: Tue, 22 Oct 2002 10:05:11 -0400
Subject: EM service lab in Taiwan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

An associate of mine is looking for a service lab in Taiwan that would be
able to pot and polish samples and examine the samples with a SEM. Any
information as to company names and/or contacts would be appreciated.

Thank you.

- David






From daemon Tue Oct 22 10:37:07 2002



From: saram-at-duke.edu
Date: Tue, 22 Oct 2002 11:25:20 -0400 (EDT)
Subject: Re: Muscle TEM

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There is at least one study showing that the important thing, as far a
shrinkage or swelling is concerned, is the osmolarity of the buffer. This
should be close to physiological (300 mOsm). Glutaraldehyde will add
about 100 mOsm for each percent, thus, a 1% glutaraldehyde solution in
buffer that would be 300 mOsm by itself would be ~400 mOsm. However, the
tonicity added by the fixaive apparently doesn't detract from the
morphology.

The 0.1 M cacodylate + 4% sucrose mentioned below will be close to the
percent we used to use with good success. I think, however, our final
sucrose concentration was 3.8%. We used to make up double strength buffer
and double strength aqueous fix and add them together 1:1; e.g., 0.2 M
cacodylate containing 6.8% sucrose and 4 % aqueous glut. Our final
concentration after mixing them 1:1 was 0.1 M caco, 3.4% sucr, 2% glut.
I think 3.5% glut is slight overkill, but won't hurt anything. Some
folks use 5%. You could also make the buffer with an increased
concentration of cacodylate, but it is expensive. 1M is sufficient
buffering capacity, and the sucrose makes up the rest of the tonicity.

Cacodylate will give a finer grained ultrastructure than phosphate, but
some folks don't want to use the arsenical. If you choose phosphate,
0.135 M phosphate buffer is physiological; you can also use less phosphate
and make up the difference with cheap sucrose. If you use something that
is ionic, like NaCl, remember that it dissociates into 2 ions and you have
to use half as much. Also, the 3.4 % sucrose translates in molarity to
0.1 M, thus if you use an ionic substitute, use molarity to calculate the
amount, not percent. A 1M solution of sucrose and a 0.5 M solution of
NaCl will give the same osmolarity.

Additionally, a small amount (2.5 mM, i.e., 0.0025 M) of calcium is good
for membranes. This is not enough to alter the osmolarity very much.

The other thing you need to do if you're interested in muscle bands (and
maybe mitochondrial size???) is to fasten the muscle bundle in a
muscle clamp before immersing in fix. Otherwise, it contracts.

This may be more than you wanted to know, but hope some of it helps.

Sara Miller


On Tue, 22 Oct 2002, Mary Gail Engle wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Marc,
} We use to routinely fix mouse muscle by immersion but used 3.5% glut. in
} .1M cacodylate at 4 degrees for 1.5 hours. The pieces need to be very
} small. Add 4% sucrose to the buffer washes. The pH should be 7.4. Osmium
} should be 1% in the same buffer at 4 degrees also.
} Good luck,
} Mary Gail
}
} At 04:48 PM 10/21/02 -0600, Marc Pypaert wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} }
} }
} } Dear List,
} }
} } I would appreciate recieveing any fixation protocol you know
} } works well for mouse muscle (extensor digitorum longus (EDL)
} } and epitrochlearus (EPI)). We use routinely immersion into
} } 2.5% glutaraldehyde in 0.1M cacodylate pH 7.4, but have had
} } often problems with mitochondrial swelling. Since this project is
} } to quantify mitochondrial density, we do need perfect and
} } reliable fixation of all mitos. Perfusion is not an option. Thanks
} } for any advise on type of fixative, buffer and temperature.
} }
} } Marc
} } --
} } Marc Pypaert
} } Department of Cell Biology,
} } Center for Cell and Molecular Imaging and
} } Ludwig Institute for Cancer Research,
} } Yale University School of Medicine
} } 333 Cedar Street
} } New Haven CT 06520
} } Tel : (203) 785 3681
} } Fax : (203) 785 7446
} }
} }
}
}
} Mary Gail Engle
} Sr. Research Laboratory Manager
} Electron Microscopy & Imaging Facility
} Health Sciences Research Bldg. 001
} University of Kentucky
} Lexington, KY 40536-0305
}
} phone 859-323-6108
} fax 859-257-9700
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Oct 22 11:00:39 2002



From: mohammed y abdulrawoof / f40z006 75760 :      mdyousuf-at-KSU.EDU.SA
Date: Tue, 22 Oct 2002 19:44:18 -0300 (GMT)
Subject: Used UA and OsO4 - Disposal

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JQ,

It has been an amazing thing receiving all this email telling me
where to order a hot plate! I guess it was not very clear in my
inquiry that we are looking for a hot plate that has a temperature
gradient across its surface.

Howard

On Tuesday, October 22, 2002, at 10:15 AM, Jim Quinn wrote:

} Howard -
}
} Why not buy one from Fisher or any other
} lab suppply house?
}
} JQ
}

} From: R. Howard Berg {rhberg-at-danforthcenter.org}


Dear All,
A bulk of used UA (in ethanol) and Osmium tetraoxide has been
left-over in plastic containers (2 liter bottles; 4 each) at my new
lab.At this place, I do not find any organized disposal system. I
believe, because of this factor,my previous collegues might have thought
it safe to leave things as such.
The question is; how to get this off my fume-hood, safely, and
to be seen off into the desert after some self disposal treatment.
Thanks in advance

M. Yousuf Abdul-Rawoof
Riyadh, Saudi Arabia.
mdyousuf-at-ksu.edu.sa



From daemon Tue Oct 22 12:05:10 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Tue, 22 Oct 2002 12:50:33 -0400
Subject: Re: Muscle TEM

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Marc,
Swelling would indicate a hypo-osmotic fixative solution, try adding
some sucrose (3.5%). I prefer
0.1 M Hepes or Phosphate buffer to cacodylate-it's too extractive. Also I
would add 3 mM Cacl2 or Mgcl2
(for the phosphate) for membrane tonicity. If you can cut very small
pieces (3mm) rapidly, that would help.
You also may think of adding paraformaldehyde to the fix. Good Luck.

Mike D.

Marc Pypaert wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear List,
}
} I would appreciate recieveing any fixation protocol you know
} works well for mouse muscle (extensor digitorum longus (EDL)
} and epitrochlearus (EPI)). We use routinely immersion into
} 2.5% glutaraldehyde in 0.1M cacodylate pH 7.4, but have had
} often problems with mitochondrial swelling. Since this project is
} to quantify mitochondrial density, we do need perfect and
} reliable fixation of all mitos. Perfusion is not an option. Thanks
} for any advise on type of fixative, buffer and temperature.
}
} Marc
} --
} Marc Pypaert
} Department of Cell Biology,
} Center for Cell and Molecular Imaging and
} Ludwig Institute for Cancer Research,
} Yale University School of Medicine
} 333 Cedar Street
} New Haven CT 06520
} Tel : (203) 785 3681
} Fax : (203) 785 7446



From daemon Tue Oct 22 12:38:53 2002



From: Maggy Piranian :      maggy-at-sparky2.esd.mun.ca
Date: Tue, 22 Oct 2002 15:03:40 -0200
Subject: evaporative coating with toxic metals

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A materials scientist asked me what happens when you use a standard
evaporative coater (either our Denton or his Varian) to coat with toxic
metals; does the metal vapor go into and stay in the diff pump oil, or
does get evacuated through the roughing pump to wherever the pump is vented
to? He was talking about things like thallium and thorium. Can anyone
help with this?

Maggy Piranian


From daemon Tue Oct 22 12:46:11 2002



From: Ann-Fook Yang :      yanga-at-agr.gc.ca
Date: Tue, 22 Oct 2002 13:37:47 -0400
Subject: Thank you, ESEM of untreated bacteria

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I like to thank all the people who responded to the posting.
Apparently, imaging untreated bacteria is not a simple exercise because bacteria are covered with polysaccharide which impede direct viewing. Image quality is quite different from conventional SEM. There is a very good wet bacteria micrograph taken with a ESEM-FEG, probably, on the web: http://www.feic.com/esem/gal6.htm
I was told that the bacteria had been fixed with glut and stored in a buffer. The suspension was filtered with a carbon coated membrane, rinsed with water before placing on a peletier stub for examination

One should not contaminate the ESEM with untreated pathogenic bacteria.
I will send the postings to anyone who asks.



AnnFook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Room 2091, Bldg. 20,
Central Experimental Farm,
Ottawa, Ontario
Canada K1A 0C6

Tel: 1-613-759-1638
Fax: 1-613-759-1701

e-mail: yanga-at-em.agr.ca



From daemon Tue Oct 22 14:22:07 2002



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Tue, 22 Oct 2002 20:04:01 +0100
Subject: Re: Alternatives to C films

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Silica films work pretty well - many years ago, when working as a
demonstrator for Philips, I had a customer who was interested in
determining the composition of small V and Nb precipitates. In
particularly, he wanted to know if they were carbides, nitrides or a
mixed carbo-nitrides. Conventional C extraction replicas were no good
so he used silica films. Worked vey nicely and confirmed - by EELS -
that they were carbo-nitrides.

Unfortunately, I can't remember how the films were made - other than
by evaporation.

Regards,
--
Larry Stoter


From daemon Tue Oct 22 14:44:30 2002



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Wed, 23 Oct 2002 08:30:16 +1300
Subject: Re: Fixation of mitochondria (Muscle TEM)

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Kia Ora

Marcs question about mitochondria is a little timely for us.

In our Unit recently we have been experiencing an increase in the
number of investigators who are wanting to quantify mitochondria
morphology, usually after some experimental change.

eg 1, changes in muscle mitochondria in resistance trained athelete's
(weight lifters) compared to endurance trained athelete's (runners)
(Human)

eg 2, changes in brain stem mitochondria after a particular
anaesthetic and neuro-surgical procedure is used (Rat)

eg 3, mitochondria changes in various tissues of hibernating hedgehog
compared to non hibernating hedgehog

eg 4, mitochondria changes in fish as they move from sea water
'lifestyle' to a fresh water 'lifestyle'.

eg 5. mitchondria changes in cultured cells after biochemically
induced. apoptosis.

These projects are all looking at morphological changes of the
mitochondria rather than chnages in the number of mitochondria.

I have some niggly concerns about these projects but I can't really
put a finger on it. Mitochondria are such sensitive souls that I am
not entirely convinced that some of the changes being noted are not
tissue dissection related or fixation related (ie some are perfused,
some are immersion fixed etc) rather than actually experiment related.

My niggles are not because of something really obvious like an
obvious osmotic problem, or delays in fixative penetration, or using
poor quality aldehyde. It is just a gut feeling. Perhaps it is
simply different 'appearances' of mitochondria in the diferent
tissues.

So I don't have a specific question, more I would interested in
peoples comments on assessing the quality of mitochondria fixation
from a morphological point of view rather than a technique point of
view.

I haven't been able to find any good reference material, apart from
that which covers technique problems, that has been able to dispel my
niggle.

Anyone got any thoughts on precautions to take in preparation when
specifically focusing on mitochondria morphology ?

Thanks

Allan


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--
-------------------------------------------------
Allan Mitchell
Technical Manager
Otago Centre for Electron Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Tue Oct 22 16:29:26 2002



From: David Walker :      dwalker-at-selway.umt.edu
Date: Tue, 22 Oct 2002 15:18:16 -0600
Subject: LM Biorad radiance 2000

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Dear List,
I am looking for a lab that uses a BioRad Radiance 2000 Confocal Microscope
that would not mind me looking over their shoulder for a few days. I have
recently been put in charge of one and I need to learn how to use it.
Unfortunately I cannot find any courses offered before March. If you can
help me out or if you know of anything offered earlier, please contact me
directly.
Thank-you
David Walker
dwalker-at-selway.umt.edu
Histology/Microscopy
CEHS
University of Montana



From daemon Tue Oct 22 16:29:26 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 22 Oct 2002 19:03:37 -0700
Subject: Re: Fixation of mitochondria (Muscle TEM)

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Maggy
I doubt if more than the minutest trace amounts get as far as that.
Most is condensed on surfaces in line-of-sight of the source, inside
the bell jar.
Is that good news or bad news?
Chris

----- Original Message -----
} From: "Maggy Piranian" {maggy-at-sparky2.esd.mun.ca}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, October 22, 2002 6:03 PM


Mitochondria is very sensitive to so many factors, which we practically
could not count. Oxidative stress is one of the major problem here. "Well
fixed" mitochondria is only "reflection" of the real structure, not real
things itself (as any other chemically fixed structure). I could imagine
the experiment, when we will take/process samples at the very identical
conditions. In this case we will probably see very small mitochondria
structure variation from sample to sample. In practice, there are so many
factors we could not count/reproduce. Therefore we expect to have a
diversity of the structure presentation from the identical organelle,
mitochondria in our case. So, we have a "distribution" of the
structure. If we are studying some "effect on mitochondria" then we have
to compare two "distributions" from control and experiment. If the
"distributions" are not cross overed, we could talk about some
changes. This is simplification of the problem of coarse. In my point of
view, it's relatively simply to compare such "distributions" on the level
of morphological changes and much, much more difficult on quantitative
level (if even possible). In general, the data analysis is not only
scientific issue, it's also ethical issue. I did see people who was
extremely smart manipulating the EM data in favor of their theories...

As for direct question in original posting, just general recommendations:
take smallest possible piece of tissue, immediately immerse them into the
fixer and cut on smaller pieces in the fixer to 1x1x1 mm with new scalpel,
then cut each piece to 0.5x0.5x0.5 mm, then move tissue in the fresh fixer
and fix 1-2 hours on ice. I usually do everything on ice, but you may try
to do first step (cutting tissue, 1st change of the fixer) at the room temp
to reduce temperature shock (use FRESH fixer for the change!). Personally,
I prefer 1x PBS instead cacodylate and 1.5% GA is enough for such small
pieces. Then I would wash tissue with 1x PBS for 30 min and fix in 1% OSO4
(in PBS or just in water) for 1 h ON ICE. 30 min water wash
thereafter. All solutions should be fresh and made from "EM grade"
chemicals and 18 MOhm "cell culture quality" deionized water. I am using
GA solutions no longer than one hour after preparation if stored on
ice. OSO4 needs to be prepared just before use and stored on ice. Time
between collecting the tissue/animal death and fixation should be 20-30
seconds or SHORTER if we are talking about "good" mitochondria fixation.
The way you kill animal have dramatic effect on mitochondria!
Good luck. Sergey.


At 08:30 AM 10/23/02 +1300, you wrote:
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From daemon Wed Oct 23 05:33:29 2002



From: Gordon Couger :      gcouger-at-couger.com
Date: Wed, 23 Oct 2002 05:19:25 -0500
Subject: MityLite

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I found a MityLigth with a 90 degree acrylic light pipe and a bulb and
reflector that give good even light through it also takes Edmonds
Scientifics 11.6 mm narrow band pass filters in the rubber boot and
works to at least 725 nm. I foolishly though away the card it came on
so if anyone has any information on it let me know.

It is a handy tool in the lab.

The set up I used is at
http://www.couger.com/microscope/projects/MityLight/MityLite.html
I found mine at Epperson Photo-Video in Okalahoma City
(405) 943-1047 for about $10. It does very good top illumination
though standard 20x objectives and works reasonably well with a
40x .65 if the objective is not too large in diameter. The light is low
angle of course.

The impressive thing about this flashlight over others like it is the higher
powered bulb and better beam of light. Still not a very even beam with out
the acrylic light pipe but better than most. With the light pipe the feild
is very even.

It is not a very good tool for photography and I don't know how far into the
ultra violet the bulb goes but it has brilliant white color that I expect
has some long UV in it. Coupled with the small Edmond filters it might make
a handy UV source for some projects. It is working well for some near infra
red work I am doing.

These little light have always been handy but the intensity of this one out
shine all those I have seen in the past.

It is a tool many of you might find handy.




From daemon Wed Oct 23 06:56:48 2002



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Wed, 23 Oct 2002 08:46:45 -0300 (ADT)
Subject: EM job classification

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Dear Microscopy :List Readers!
Thank you very much for all the responses that I received from you
to my inquiry. They all were very helpful. I am starting a process of
an appeal and it might be a long way but I think it is worth pursuing.
Thanks again
Dorota



From daemon Wed Oct 23 07:52:15 2002



From: Coalbunny :      coalbunny-at-vcn.com
Date: Wed, 23 Oct 2002 06:43:16 -0600
Subject: Re: MityLite

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Cool job Gordon! And awesome pics! Funny thing is, I never thought
that KMnO4 was golden under a scope. Or was I looking at the wrong
stuff?
Carl


Gordon Couger wrote:
}
} I found a MityLigth with a 90 degree acrylic light pipe and a bulb and
} reflector that give good even light through it also takes Edmonds
} Scientifics 11.6 mm narrow band pass filters in the rubber boot and
} works to at least 725 nm. I foolishly though away the card it came on
} so if anyone has any information on it let me know.
}
} It is a handy tool in the lab.
}
} The set up I used is at
} http://www.couger.com/microscope/projects/MityLight/MityLite.html
} I found mine at Epperson Photo-Video in Okalahoma City
} (405) 943-1047 for about $10. It does very good top illumination
} though standard 20x objectives and works reasonably well with a
} 40x .65 if the objective is not too large in diameter. The light is low
} angle of course.
}
} The impressive thing about this flashlight over others like it is the higher
} powered bulb and better beam of light. Still not a very even beam with out
} the acrylic light pipe but better than most. With the light pipe the feild
} is very even.
}
} It is not a very good tool for photography and I don't know how far into the
} ultra violet the bulb goes but it has brilliant white color that I expect
} has some long UV in it. Coupled with the small Edmond filters it might make
} a handy UV source for some projects. It is working well for some near infra
} red work I am doing.
}
} These little light have always been handy but the intensity of this one out
} shine all those I have seen in the past.
}
} It is a tool many of you might find handy.

--
"I learned this, at least, by my experiment: That if one advances
confidently in the direction of one's dreams, and endeavors to live the
life one has imagined, one will meet with a success unexpected in common
hours." Henry David Thoreau


From daemon Wed Oct 23 08:27:30 2002



From: ROSSCAC-at-aol.com
Date: Wed, 23 Oct 2002 09:14:00 EDT
Subject: TEM and Mitochondria

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Good day listserver,
Here's my opinion:
I agree that mitochondria are very sensitive to what occurs during oxidative
stress, death, fixation, processing, etc --- so, the only thing left is too
COMPARE controls versus treated - IF the tissues are handled the same in all
respects then someone with a "trained eye" can see thru the artifacts and
process whether there really is a difference. I for one look at each group
separately then compare the groups looking at the quality of the mitochondria
and then the quantity. Size of mitochondria can often be so variable between
animals (animal variation and tissue variation) that it is best to look at
the total surface area in the cell that contains mitochondria - by doing
this, you should be able to compare groups as well.

Connie A. Cummings, DVM, PhD
Staff Pathologist
Pathology Associates - A Charles River Company
4915-D Prospectus Drive
Durham, NC 27713
919-544-5257


From daemon Wed Oct 23 11:01:34 2002



From: John Foust :      jfoust-at-threedee.com
Date: Wed, 23 Oct 2002 10:40:24 -0500
Subject: Re: WORM drives

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At 02:11 PM 10/16/2002 +0100, Richard Beanland wrote:
} Our LEO 360FE SEM computer saves images on a Panasonic LF-7300 WORM drive.
} It doesn't seem feasible to connect the computer to our intranet directly
} (too old/difficult!) but a relatively straighforward way to get at the
} images is to install an identical WORM drive on a PC which is networked.

What OS, what hardware is the SEM computer? It may be possible.
As someone pointed out, WinNT may handle it directly. We'd
need to confirm which filesystem is on the MO.

Another alternative I found with a little googling is :
http://www.instar.com/products/software/pc_optic.html

- John



From daemon Wed Oct 23 11:46:19 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 23 Oct 2002 09:37:19 -0700
Subject: Re: evaporative coating with toxic metals

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On Tuesday, October 22, 2002, at 02:08 PM, Chris Jeffree wrote:

} I doubt if more than the minutest trace amounts get as far as that.
} Most is condensed on surfaces in line-of-sight of the source, inside
} the bell jar.
} Is that good news or bad news?
} }

} } From: "Maggy Piranian" {maggy-at-sparky2.esd.mun.ca}
} } Subject: evaporative coating with toxic metals
} }
} } A materials scientist asked me what happens when you use a standard
} } evaporative coater (either our Denton or his Varian) to coat with
} } toxic
} } metals; does the metal vapor go into and stay in the diff pump oil,
} } or
} } does get evacuated through the roughing pump to wherever the pump is
} } vented
} } to? He was talking about things like thallium and thorium. Can
} } anyone
} } help with this?
} }
Dear Maggy & Chris,
I agree that most of the metal will end up on the bell jar and other
surfaces inside the evaporator, but there will be some material that is
drawn through the pumps. Some of this will remain in the oil and some
will go out the exhaust, so both should be treated as hazardous. If
these toxic metals cannot be evaporated in an instrument dedicated to
this (and placed in a hood), the evaporator should be thoroughly
cleaned and the pump oils should be changed before returning it to
routine use. Good luck.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Wed Oct 23 11:50:06 2002



From: =?iso-8859-1?q?Jeremy=20Sanderson?= :      jb_sanderson-at-yahoo.com
Date: Wed, 23 Oct 2002 17:42:33 +0100 (BST)
Subject: File formats

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Hello listers,
I have a question from a colleague off-list, and am
also interested in the solution:

We have a 4.5Mb .mov file captured down the microscope
from a digital camera which we'd like to change to
.avi format, and then compressed. The compression is
primarily so that it can be inserted into a webpage,
and also be small enough to (hopefully) fit onto a
standard 1.4Mb floppy disk.

Thanks in advance for your help

Jeremy Sanderson

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com


From daemon Wed Oct 23 11:58:56 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 23 Oct 2002 09:50:59 -0700
Subject: Re: Used UA and OsO4 - Disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Tuesday, October 22, 2002, at 03:44 PM, mohammed y abdulrawoof /
f40z006 75760 wrote:

} A bulk of used UA (in ethanol) and Osmium tetraoxide has been
} left-over in plastic containers (2 liter bottles; 4 each) at my new
} lab.At this place, I do not find any organized disposal system. I
} believe, because of this factor,my previous collegues might have
} thought
} it safe to leave things as such.
} The question is; how to get this off my fume-hood, safely, and
} to be seen off into the desert after some self disposal treatment.
} Thanks in advance
}
Dear Mohammed,
If there are no regulations about disposal, small amounts of UA are
not too big a problem. Some places in the US allow a small amount of
UA to be discarded by washing them down a sink (others require special
disposal). OsO4 is volatile and reacts with organic material, so it is
more dangerous. Cover the surface with corn oil (or any unsaturated
oil). This will prevent any vapors from getting into the air, and
eventually all the OsO4 will react with the oil. I would check again
whether there are safety regulations about disposal of hazardous waste.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Wed Oct 23 12:42:16 2002



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 23 Oct 2002 13:47:21 -0700
Subject: Re: Ask-A-Microscopist:Calibration Standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-----Original Message-----
} From: Petersen, Maureen A.
Sent: Wednesday, October 23, 2002 10:39 AM
To: 'ListServer-at-MSA.Microscopy.Com'


Dear Peter,

MME provides a set of fluorescence reference slides called FluorRef. Visit our website www.MicroscopyEducation.com for spectral and other details.

Caveat: MME has a financial interest in this product.

Best regards,
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suite 102
Springfield, MA 01118
PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com

~-at-~-at-~~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-
Optimizing Light Microscopy for Biological and Clinical Labs is still available
in individual copies or classroom size orders. Visit www.MicroscopyEducation.com
for details.
~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-

At 02:24 PM 10/21/02 -0500, pkrsko-at-stevens-tech.edu wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Oct 23 13:39:49 2002



From: tbargar-at-unmc.edu
Date: Wed, 23 Oct 2002 13:24:55 -0500
Subject: Negative staining of pox viruses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have any experience and protocols for doing negative staining
of pox viruses?

Tom Bargar
UNMC-CEMRF
402-559-7347
tbargar-at-unmc.edu




From daemon Wed Oct 23 14:36:21 2002



From: Bob Harris :      bharris-at-uoguelph.ca
Date: Wed, 23 Oct 2002 15:22:30 -0400
Subject: Balzers freeze etchers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:

I have 2 freeze etchers, a 360M which I want to keep, and a 400D which I
don't. I've advertised it as available but the only takers want only the
pumps. My question is what parts should I keep from the 400 to use on the 360
in case of breakdown? bob harris

Guelph Regional STEM Facility
Dept. of Microbiology
Univ of Guelph
Guelph,On, Canada
N1G 2W1
ph: 519-824-4120 ext. 6409
Fax: 519-837-1802


From daemon Wed Oct 23 16:55:58 2002



From: saram-at-duke.edu
Date: Wed, 23 Oct 2002 17:39:15 -0400 (EDT)
Subject: Re: Negative staining of pox viruses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


----- Message Text -----
Yes. What do you want to know? And why?

If you are thinking of smallpox virus detection, there are numerous
considerations. It's not a simple matter of plopping some onto a grid and
putting it into the EM.

For negative staining methods, see

MA Hayat and SE Miller. 1990. Negative Staining: Application and Methods.
McGraw Hill, New York.

For virus identification, see

Doane FW, Anderson N. 1986. Electron Microscopy in Diagnostic Virology:
A Practical Guide and Atlas, Cambridge University Press, New York.

Miller SE. 1986. Detection and identification of viruses by electron
microscopy. J. Electron Microsc. Tech. 4:265-301.

Miller SE 1995. Diagnosis of viral infection by electron microscopy.
In EH Lennette, DA Lennette, and ET Lennette (eds.), Diagnostic
Procedures for Viral, Rickettsial and Chlamydial Infections, American
Public Health Assoc., Washington, D. C. pp. 35-76.

Palmer E, Martin ML. 1988. Electron Microscopy in Viral Diagnosis, CRC
Press, Boca Raton, FL.

Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, GP Martelli,
Mayo MA, Summers MD. 1995. Virus Taxonomy. Sixth Report of the
International Committee on Taxonomy of Viruses. Springer-Verlag, New
York. (There's a 7th report now out.)

Address at bottom. Feel free to call me if you want.

Sara Miller





On Wed, 23 Oct 2002 tbargar-at-unmc.edu-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone have any experience and protocols for doing negative staining
} of pox viruses?
}
} Tom Bargar
} UNMC-CEMRF
} 402-559-7347
} tbargar-at-unmc.edu
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Oct 23 16:55:58 2002



From: Barbara Plowman :      Bplowman-at-sf.uop.edu
Date: Wed, 23 Oct 2002 14:39:42 -0700
Subject: Re: MityLite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


MityLite is made in the USA by Pelican Products
23215 Early Avenue, Torrance, CA 90505.
Their phone number is (310) 326-4700 and (310) 326-3311

Barbara Plowman
UOP School of Dentistry
2155 Webster Rm 632
San Francisco, CA 94115
Bplowman-at-sf.uop.edu



From daemon Wed Oct 23 18:40:19 2002



From: Mark Johnson :      mark_johnson-at-ncsu.edu
Date: Wed, 23 Oct 2002 19:30:26 -0400
Subject: SEM/CL lifetime measurements

Contents Retrieved from Microscopy Listserver Archives
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I have a JEOL SEM with a CL (Cathodoluminescence) attachment. I
primarily use this system for CL optical characterization of wide
bandgap semiconductors in the 200-300nm wavelength range. (High mole
fraction Aluminum Gallium Nitride). This system is analogous to PL
(photoluminescence) for materials where the band-to-band excitation
would be inconvenient for all but excimer laser sources.

I would like to know if anyone has experience or references for using
an SEM to measure carrier recombination lifetimes by CL. I would
think that the beam could be chopped with a high speed beam blanking
system,
and the CL intensity measured as a function of time. How fast is the
fastest beam blankers and would it be feasible to perform such an
experiment.

Thanks-

Mark






From daemon Wed Oct 23 20:35:36 2002



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Wed, 23 Oct 2002 21:24:28 -0600
Subject: Muscle TEM - thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank a lot for all the helpful advise about fixation of skeletal
muscle. I am still trying to digest all the info, but it seems that
the key is to add paraformaldehyde so that the fixative will
diffuse quicker through the sample and reduces damage to
mitochondria. There are of course many other factors involved -
hopefully I will one day find the time to try them all. Thanks
for now!

Marc
--
Marc Pypaert
Department of Cell Biology,
Center for Cell and Molecular Imaging and
Ludwig Institute for Cancer Research,
Yale University School of Medicine
333 Cedar Street
New Haven CT 06520
Tel : (203) 785 3681
Fax : (203) 785 7446




From daemon Thu Oct 24 06:09:24 2002



From: michael shaffer :      michael-at-shaffer.net
Date: Thu, 24 Oct 2002 08:26:02 -0230
Subject: Visilog file format

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have software capable of 16bit imaging, but the only format to which it
writes 16bits is for Visilog software. Is anyone aware of software which
can read (or convert) the Visilog format?

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com




From daemon Thu Oct 24 08:33:18 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 24 Oct 2002 08:23:17 -0500
Subject: Materials-staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi folks,
I have a user who has a molded plastic sample containing substances
having end groups of carboxylic acid or amines. Do you have any suggestions
for differentially staining these groups so that they can be identified from
the background material.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Thu Oct 24 09:25:04 2002



From: Spehner Daniele :      daniele.spehner-at-efs-alsace.fr (by way of
Date: Thu, 24 Oct 2002 09:17:03 -0500
Subject: RE: Negative staining of pox viruses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tom,
I am a poxvirologist since 30 years. It is very easy to see them under the
EM after negative staining. The negative staining is done as usual : put
your virus (10 µl) on a formvar-carbon coated grid for one min. then add the
negative stain, uranyl acetate at a concentration of 2% in water, for 30-45
seconds. Don't wash, just dry the grid and observe. It is easy to
distinguish the orf virus due to its "ball of wool-like appearance" (surface
tubules) from other poxviruses.
P.S. just a reminder : smallpox is officially eradicated since 1980 and only
two laboratories in the world are allowed to work with this virus, the CDC
in Atlanta and Vector in Novosibirsk.
Hope that this helps,
daniele

--------------------------------------------
Danièle Spehner, INSERM EPI 99-08 - EFS-Alsace
10 rue Spielmann - 67065 STRASBOURG
tel : 03 88 21 25 25 - fax : 03 88 21 25 44
e-mail : daniele.spehner-at-efs-alsace.fr
------------------------------------


-----Message d'origine-----
De : "tbargar-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"tbargar-at-unmc.edu"-at-sparc5.microscopy.com]
Envoyé : mercredi 23 octobre 2002 20:25
À : Microscopy-at-sparc5.microscopy.com
Objet : Negative staining of pox viruses


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anyone have any experience and protocols for doing negative staining
of pox viruses?

Tom Bargar
UNMC-CEMRF
402-559-7347
tbargar-at-unmc.edu


From daemon Thu Oct 24 09:31:12 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Thu, 24 Oct 2002 10:23:30 -0400
Subject: Re: Visilog file format

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} We have software capable of 16bit imaging, but the only format to which it
} writes 16bits is for Visilog software. Is anyone aware of software which
} can read (or convert) the Visilog format?
}
} cheerios ... shAf :o)
} Avalon Peninsula, Newfoundland
} www.micro-investigations.com

----------


XnView probably has support for the most formats (imports ~360 formats, exports ~40 formats) I've seen in freeware (for non-commercial/non-profit/educational), and also runs on quite a few OS's.

I checked the website http://www.xnview.com/ , and it does indeed support importing of im5 (Visilog) format.

-Kevin


Kevin Frischmann, Laboratory Manager
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: 212-313-7975
Fax: 212-496-3480
email: kfrisch-at-amnh.org



From daemon Thu Oct 24 11:20:04 2002



From: Mike Peters :      petersmc-at-stanford.edu
Date: Thu, 24 Oct 2002 09:06:28 -0700
Subject: Double Labeling Storage Query

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
I have cryosections that have been double labeled using two different
combinations of HRP chromogens (Vector VIP, SG, & DAB). I want to
correlate the LM with EM. However, I have found that (despite what Vector
says) that the chromogens leach out in buffer, water, and ethanol over the
course of a weekend. Vector suggests that I dehydrate and mount the
sections in Permamount (although I would prefer DPX ), then examine under
LM, choose the sections that I want to run up for EM, and subsequently
remove sections, rehydrate, & continue as normal. Sounds like one hell of
a way to end up with crap. I can't imagine sections surviving without
extreme damage. Any suggestions? What if I select the ones for EM and
then osmicate. Will that prevent loss of the HRP substrate?

Mike Peters
Stanford Center for Pain Research



From daemon Thu Oct 24 11:38:57 2002



From: Bruce Ingber :      bingber-at-srrc.ars.usda.gov
Date: Thu, 24 Oct 2002 11:30:58 -0500
Subject: Re: Used UA and OsO4 - Disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I wouldn't try washing small amounts of UA down the sink. We had a scientist do this ten or more years ago. He hadn't flushed the drain with enough water though. When the lab was renovated several years after that time, plumbers who dismantled the sink found a distinct yellow coating inside the U joint connection. Much to their (and safety rep) displeasure this coating was not surprisingly radioactive.

Treat the used UA solution as heavy metal waste for proper disposal. As for weighing UA, we insist appropriate safety precautions (gloves, etc.) be followed along with placing a Beta shield in front of the product and balance. The concentrated UA is sufficiently "hot" to warrant this approach.{CYB}

Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
bingber-at-srrc.ars.usda.gov
504-286-4270 phone
504-286-4419 fax

} } } Bill Tivol {tivol-at-caltech.edu} 10/23/02 11:50AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



On Tuesday, October 22, 2002, at 03:44 PM, mohammed y abdulrawoof /
f40z006 75760 wrote:

} A bulk of used UA (in ethanol) and Osmium tetraoxide has been
} left-over in plastic containers (2 liter bottles; 4 each) at my new
} lab.At this place, I do not find any organized disposal system. I
} believe, because of this factor,my previous collegues might have
} thought
} it safe to leave things as such.
} The question is; how to get this off my fume-hood, safely, and
} to be seen off into the desert after some self disposal treatment.
} Thanks in advance
}
Dear Mohammed,
If there are no regulations about disposal, small amounts of UA are
not too big a problem. Some places in the US allow a small amount of
UA to be discarded by washing them down a sink (others require special
disposal). OsO4 is volatile and reacts with organic material, so it is
more dangerous. Cover the surface with corn oil (or any unsaturated
oil). This will prevent any vapors from getting into the air, and
eventually all the OsO4 will react with the oil. I would check again
whether there are safety regulations about disposal of hazardous waste.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu





From daemon Thu Oct 24 11:43:42 2002



From: saram-at-duke.edu
Date: Thu, 24 Oct 2002 12:24:15 -0400 (EDT)
Subject: RE: Negative staining of pox viruses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is good advice for looking at orf and other poxviruses. However, as
I alluded to in my last email, if you want to look at poxvirus zoonoses
or perhaps molluscum congatiosum, fine, but smallpox is a different
story. If your purpose is to become a surveillance lab for smallpox,
there are strict guidelines being set up by the CDC. Please contact me
offline.

Sara Miller


On Thu, 24 Oct 2002, Spehner Daniele wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Tom,
} I am a poxvirologist since 30 years. It is very easy to see them under the
} EM after negative staining. The negative staining is done as usual : put
} your virus (10 µl) on a formvar-carbon coated grid for one min. then add the
} negative stain, uranyl acetate at a concentration of 2% in water, for 30-45
} seconds. Don't wash, just dry the grid and observe. It is easy to
} distinguish the orf virus due to its "ball of wool-like appearance" (surface
} tubules) from other poxviruses.
} P.S. just a reminder : smallpox is officially eradicated since 1980 and only
} two laboratories in the world are allowed to work with this virus, the CDC
} in Atlanta and Vector in Novosibirsk.
} Hope that this helps,
} daniele
}
} --------------------------------------------
} Danièle Spehner, INSERM EPI 99-08 - EFS-Alsace
} 10 rue Spielmann - 67065 STRASBOURG
} tel : 03 88 21 25 25 - fax : 03 88 21 25 44
} e-mail : daniele.spehner-at-efs-alsace.fr
} ------------------------------------
}
}
} -----Message d'origine-----
} De : "tbargar-at-unmc.edu"-at-sparc5.microscopy.com
} [mailto:"tbargar-at-unmc.edu"-at-sparc5.microscopy.com]
} Envoyé : mercredi 23 octobre 2002 20:25
} À : Microscopy-at-sparc5.microscopy.com
} Objet : Negative staining of pox viruses
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone have any experience and protocols for doing negative staining
} of pox viruses?
}
} Tom Bargar
} UNMC-CEMRF
} 402-559-7347
} tbargar-at-unmc.edu
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Oct 24 12:00:23 2002



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Thu, 24 Oct 2002 13:55:04 -0300
Subject: European EM supplies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I'm looking for a European vendor for EM supplies. I have colleagues
in Germany who want to buy some things for an upcoming visit, but
they must purchase everything in Europe. I would prefer a vendor with
an English online catalog so I can point out the correct things to order.

Please contact me off list.

Thanks in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Thu Oct 24 12:20:36 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Thu, 24 Oct 2002 10:13:12 -0700
Subject: Re: Heavy metal accumulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Wednesday, October 23, 2002, at 10:12 AM, Petersen, Maureen A. (by
way of MicroscopyListserver) wrote:

} Perhaps someone can comment on my following concern.
}
} I am having an analysis of new growth hair ( cut from close to the
} scalp), for a determination of whether or not there are indications of
} abnormal metal accumulation in my body, as measured in hair. Since
} that sample was taken, I have begun to consider the possibility of
} uranium and lead showing up, since I have worked as an electron
} microscopist for 20 years. I think I treat all hazardous materials
} properly, but this has been a nagging question in my mind. Does anyone
} have any knowledge, insight, or comments on this being a possible
} problem?
}
Dear Maurine,
I don't know what fraction of U or Pb is sequestered in the hair--I
think that Pb ends up in bone--so I could not interpret a negative
result. Of course, high levels of any heavy metal in your hair would
be bad. I also don't know the environment around Gainesville, so I
wouldn't know if the soil has higher-than-average content of Pb or U
(There is a formation called the Reading Prong in upstate NY and PA
that is high in U, and I found measurable U in Hudson River sediment.).
You could be as careful as possible in the lab and still accumulate
metals from drinking water, dust particles, or other environmental
sources.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Thu Oct 24 13:03:37 2002



From: Paul Voyles :      pvoyles-at-bell-labs.com
Date: Thu, 24 Oct 2002 13:45:54 -0400
Subject: Re: File formats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 05:42 PM 10/23/2002 +0100, you wrote:
} Hello listers,
} I have a question from a colleague off-list, and am
} also interested in the solution:
}
} We have a 4.5Mb .mov file captured down the microscope
} from a digital camera which we'd like to change to
} .avi format, and then compressed. The compression is
} primarily so that it can be inserted into a webpage,
} and also be small enough to (hopefully) fit onto a
} standard 1.4Mb floppy disk.

You don't say what computer platform you want to use for this task. I have
a solution for Windows which involves only free software.

First convert from Quicktime to avi using a program called Bink. It is
part of the RadVideo tools, available from

{http://www.radgametools.com/bnkmain.htm}

This will create an uncompressed avi movie, which will be a very large
file. That file can be compressed using VirtualDub:

{http://www.virtualdub.org/}

using far more compression schemes, file formats, and options than I even
vaguely understand.

I believe that Quicktime Pro, available from Apple for a relatively small
amount of money will do the conversion in one step, but I have never tried it.

Good luck!



Best wishes,
Paul

Paul Voyles
Post-doctoral MTS
Bell Laboratories
700 Mountain Ave, Rm 1D-437
Murray Hill, NJ 07974-0636
voice: (908) 582-4399
fax: (908) 582-4868



From daemon Thu Oct 24 14:15:22 2002



From: zaluzec-at-microscopy.com
Date: Thu, 24 Oct 2002 14:08:03 -0500
Subject: Administrivia: Forged Email Addresses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

Just a heads up. I have been receiving reports of junk/spam mail
arriving from "microscopy-at-sparc5.microsocpy.com" as well as
variants thereof.

Please be aware, that the current generation of Email spammers and
virus software can insert a forged or fake FROM: address. There have
been a number of instances where the Microscopy Listserver address
has been forged.

Just because the FROM address looks like it is originating with
someone/someplace you know, please be aware it could be a
easily faked/forged. This unfortunately, is not something that
I can stop, as the mail does not "originate" or even flow through
this server. Thus it completely bypasses my spam/junk mail
filters.

One way to sort this out is to look at the
Email header lines, and trace the route backward. This works
at least 50% of the time, however, the truely inspired hacker
can insert information into the routing message to confuse the issue.

Just to give you an idea of the magnitude. The SPAM/Junk Mail filter
for the LIstserver is stopping ~ 15 junk mail postings/day. It is
getting to be a never ending battle.

Remember if your posting inadvertently triggers the Spam filter
follow the directions on the Rejected Mail message you get exactly. So that
we can get you back into the system.

Nestor
Your Friendly Neighborhood SysOp




From daemon Thu Oct 24 14:25:02 2002



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Thu, 24 Oct 2002 15:14:18 -0400
Subject: SEM images - black blotches

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Hi gang - had some forams that someone had glued to a stub - sputter
coated with AuPd - on some specimens black blotches appeared - doesn't
appear to be from coating. Could this be from the glue or what could it
be? Tried to capture images at high and low vacuum with SEI and BEI
Any suggestions would be appreciated.
Thanks
Barb



From daemon Thu Oct 24 14:36:01 2002



From: saram-at-duke.edu
Date: Thu, 24 Oct 2002 15:21:44 -0400 (EDT)
Subject: RE: Negative staining of pox viruses

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Oops. Sorry for the misspelling in my last communication; fingers weren't
connected to brain. I was rushing out the door and didn't proof it.

Correct:
molluscum contagiosum



Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Thu Oct 24 15:32:30 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 24 Oct 2002 15:21:48 -0500
Subject: 35 vs 45 degree diamond knives

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Any opinions out there on the advantages and disadvantages of a 35
degree diamond knife compared to a 45 degree one? 35's are supposed
to cause less compression - is that found in the real world? TIA, tom


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu Oct 24 15:32:31 2002



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Thu, 24 Oct 2002 20:53:52 +0100
Subject: Re: evaporative coating with toxic metals

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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Personally, given the toxicity of Thallium and the ignorance of its
effects usually exhibited by a general medical doctor, I would be
extremely cautious about using it in an evaporation system.

Regards
--
Larry Stoter


From daemon Thu Oct 24 15:47:55 2002



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Thu, 24 Oct 2002 15:40:44 -0500
Subject: RE: Heavy metal accumulation

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Some interesting work on this thread can be found at:

http://www.anl.gov:80/OPA/whatsnew/beethovenstory.htm




"Eternal vigilance is the price of liberty."-- Wendell Phillips


Jeffrey A Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439
(630) 252-5594
FAX: (630) 972-4438


} ----------
} From: Bill Tivol
} Sent: Thursday, October 24, 2002 12:13 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Re: Heavy metal accumulation
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} On Wednesday, October 23, 2002, at 10:12 AM, Petersen, Maureen A. (by
} way of MicroscopyListserver) wrote:
}
} } Perhaps someone can comment on my following concern.
} }
} } I am having an analysis of new growth hair ( cut from close to the
} } scalp), for a determination of whether or not there are indications of
} } abnormal metal accumulation in my body, as measured in hair. Since
} } that sample was taken, I have begun to consider the possibility of
} } uranium and lead showing up, since I have worked as an electron
} } microscopist for 20 years. I think I treat all hazardous materials
} } properly, but this has been a nagging question in my mind. Does anyone
} } have any knowledge, insight, or comments on this being a possible
} } problem?
} }
} Dear Maurine,
} I don't know what fraction of U or Pb is sequestered in the hair--I
} think that Pb ends up in bone--so I could not interpret a negative
} result. Of course, high levels of any heavy metal in your hair would
} be bad. I also don't know the environment around Gainesville, so I
} wouldn't know if the soil has higher-than-average content of Pb or U
} (There is a formation called the Reading Prong in upstate NY and PA
} that is high in U, and I found measurable U in Hudson River sediment.).
} You could be as careful as possible in the lab and still accumulate
} metals from drinking water, dust particles, or other environmental
} sources.
} Yours,
} Bill Tivol
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}


From daemon Thu Oct 24 16:08:06 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 24 Oct 2002 17:59:07 -0400
Subject: Re:TEM of yeast

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Go to www.tedpella.com or their international site www.pelcoint.com

Diane G. Miller
Miller Consultant Service
503-784-6444
millerd-at-coho.net

----- Original Message -----
} From: "James M. Ehrman" {jehrman-at-mta.ca}
To: "Microscopy Listserv" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, October 24, 2002 9:55 AM


Hi All,
I have just gotten a request to run some samples of yeast (Candida
sp) up for TEM. Normally I deal with tissues and (eukaryote) cell
cultures. Can anyone out there give me a few hints about how to deal
with these wee little beasties, that might differ from a "standard"
protocol?
Thanks in advance,
Lee
--
Lee Cohen-Gould
Electron & Optical Microscopy Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207


From daemon Thu Oct 24 18:49:44 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 24 Oct 2002 16:40:29 -0700
Subject: Re: 35 vs 45 degree diamond knives

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Dear Tom
35 is great for cryo-sectioning. From another hand, it's more fragile and
needs more attention. For plastic sectioning, I would suggest: if you have
only one knife - it should be 45, if you rich enough to have let say 5
knifes, one of them may be 35 for some special work. As I told before, 35
is superior for cryo-sectioning of the frozen tissue. Sergey

At 01:21 PM 10/24/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Oct 24 18:52:18 2002



From: sstouden-at-thelinks.com
Date: Thu, 24 Oct 2002 18:45:36 -0500 (CDT)
Subject: Re: Fixation of mitochondria (Muscle TEM)

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pardon me, but i would like to ask very simple question, which I am sure
everyone else on this list but me is able to answer,

what is the morphology of a standard mitochrondium:

how many standard mitochrondia structures are there?
do structures appear and disappear within the same mitochrondrium
Does cell type of origin change the standard of the structure?
Is there a mitochrondria database somewhere?
what about pore type intra mitochondrial and intercompartmental?
pore distribution function and density seems to be the big determinate of
mitochrondiral differentiation and function.
what are they.
the big structure we all know, it the little ones, that I am talking
about, some that are sometimes there and sometimes not.



how are the different structures separately classified in each such
mitochrondria?
is there a measurement technique to determine exact location and volume of
these intra mitochrondrial structures?

is there a noticeable by experimental method difference between
mitochrondria of one source or the other other.

thanks for indulging my ignorance.
sterling

On Tue, 22 Oct 2002, Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Mitochondria is very sensitive to so many factors, which we practically
} could not count. Oxidative stress is one of the major problem here. "Well
} fixed" mitochondria is only "reflection" of the real structure, not real
} things itself (as any other chemically fixed structure). I could imagine
} the experiment, when we will take/process samples at the very identical
} conditions. In this case we will probably see very small mitochondria
} structure variation from sample to sample. In practice, there are so many
} factors we could not count/reproduce. Therefore we expect to have a
} diversity of the structure presentation from the identical organelle,
} mitochondria in our case. So, we have a "distribution" of the
} structure. If we are studying some "effect on mitochondria" then we have
} to compare two "distributions" from control and experiment. If the
} "distributions" are not cross overed, we could talk about some
} changes. This is simplification of the problem of coarse. In my point of
} view, it's relatively simply to compare such "distributions" on the level
} of morphological changes and much, much more difficult on quantitative
} level (if even possible). In general, the data analysis is not only
} scientific issue, it's also ethical issue. I did see people who was
} extremely smart manipulating the EM data in favor of their theories...
}
} As for direct question in original posting, just general recommendations:
} take smallest possible piece of tissue, immediately immerse them into the
} fixer and cut on smaller pieces in the fixer to 1x1x1 mm with new scalpel,
} then cut each piece to 0.5x0.5x0.5 mm, then move tissue in the fresh fixer
} and fix 1-2 hours on ice. I usually do everything on ice, but you may try
} to do first step (cutting tissue, 1st change of the fixer) at the room temp
} to reduce temperature shock (use FRESH fixer for the change!). Personally,
} I prefer 1x PBS instead cacodylate and 1.5% GA is enough for such small
} pieces. Then I would wash tissue with 1x PBS for 30 min and fix in 1% OSO4
} (in PBS or just in water) for 1 h ON ICE. 30 min water wash
} thereafter. All solutions should be fresh and made from "EM grade"
} chemicals and 18 MOhm "cell culture quality" deionized water. I am using
} GA solutions no longer than one hour after preparation if stored on
} ice. OSO4 needs to be prepared just before use and stored on ice. Time
} between collecting the tissue/animal death and fixation should be 20-30
} seconds or SHORTER if we are talking about "good" mitochondria fixation.
} The way you kill animal have dramatic effect on mitochondria!
} Good luck. Sergey.
}
}
} At 08:30 AM 10/23/02 +1300, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Kia Ora
} }
} } Marcs question about mitochondria is a little timely for us.
} }
} } In our Unit recently we have been experiencing an increase in the number
} } of investigators who are wanting to quantify mitochondria morphology,
} } usually after some experimental change.
} }
} } eg 1, changes in muscle mitochondria in resistance trained athelete's
} } (weight lifters) compared to endurance trained athelete's (runners) (Human)
} }
} } eg 2, changes in brain stem mitochondria after a particular anaesthetic
} } and neuro-surgical procedure is used (Rat)
} }
} } eg 3, mitochondria changes in various tissues of hibernating hedgehog
} } compared to non hibernating hedgehog
} }
} } eg 4, mitochondria changes in fish as they move from sea water 'lifestyle'
} } to a fresh water 'lifestyle'.
} }
} } eg 5. mitchondria changes in cultured cells after biochemically induced.
} } apoptosis.
} }
} } These projects are all looking at morphological changes of the
} } mitochondria rather than chnages in the number of mitochondria.
} }
} } I have some niggly concerns about these projects but I can't really put a
} } finger on it. Mitochondria are such sensitive souls that I am not
} } entirely convinced that some of the changes being noted are not tissue
} } dissection related or fixation related (ie some are perfused, some are
} } immersion fixed etc) rather than actually experiment related.
} }
} } My niggles are not because of something really obvious like an obvious
} } osmotic problem, or delays in fixative penetration, or using poor quality
} } aldehyde. It is just a gut feeling. Perhaps it is simply different
} } 'appearances' of mitochondria in the diferent tissues.
} }
} } So I don't have a specific question, more I would interested in peoples
} } comments on assessing the quality of mitochondria fixation from a
} } morphological point of view rather than a technique point of view.
} }
} } I haven't been able to find any good reference material, apart from that
} } which covers technique problems, that has been able to dispel my niggle.
} }
} } Anyone got any thoughts on precautions to take in preparation when
} } specifically focusing on mitochondria morphology ?
} }
} } Thanks
} }
} } Allan
} }
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear List,
} } }
} } } I would appreciate recieveing any fixation protocol you know
} } } works well for mouse muscle (extensor digitorum longus (EDL)
} } } and epitrochlearus (EPI)). We use routinely immersion into
} } } 2.5% glutaraldehyde in 0.1M cacodylate pH 7.4, but have had
} } } often problems with mitochondrial swelling. Since this project is
} } } to quantify mitochondrial density, we do need perfect and
} } } reliable fixation of all mitos. Perfusion is not an option. Thanks
} } } for any advise on type of fixative, buffer and temperature.
} } }
} } } Marc
} } } --
} } } Marc Pypaert
} } } Department of Cell Biology,
} } } Center for Cell and Molecular Imaging and
} } } Ludwig Institute for Cancer Research,
} } } Yale University School of Medicine
} } } 333 Cedar Street
} } } New Haven CT 06520
} } } Tel : (203) 785 3681
} } } Fax : (203) 785 7446
} }
} } --
} } -------------------------------------------------
} } Allan Mitchell
} } Technical Manager
} } Otago Centre for Electron Microscopy
} } C/-Department of Anatomy and Structural Biology
} } School of Medical Sciences
} } P.O. Box 913
} } Dunedin
} } New Zealand
} }
} } Phone (03) 479 5642 or 479 7301
} } Fax (03) 479 7254
} }
} } Unit: http://www.otago.ac.nz/anatomy/emunit/
} } Department: http://anatomy.otago.ac.nz/
} }
} }
} }
} } "Life is a gift, don't waste it"
}
}



From daemon Thu Oct 24 19:28:36 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 24 Oct 2002 20:20:42 -0500
Subject: Sputter coater problem

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Barbara Maloney wrote:
====================================================
Hi gang - had some forams that someone had glued to a stub - sputter
coated with AuPd - on some specimens black blotches appeared - doesn't
appear to be from coating. Could this be from the glue or what could it
be? Tried to capture images at high and low vacuum with SEI and BEI
Any suggestions would be appreciated.
===================================================
If there was a "soot" like deposit (what you called black blotches) around
the chamber, then in all probability, a change of your oil in the rotary
vane pump will make the problem go away.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Thu Oct 24 23:37:45 2002



From: Mark G BLACKFORD :      mgb-at-ansto.gov.au
Date: Fri, 25 Oct 2002 14:26:24 +1000
Subject: electron diffraction simulation software

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Hi All,

I would like to know what software people are using for simulating dynamical and kinematical electron diffraction patterns.

We are interested in selected area electron diffraction at the moment but may eventually wish to do convergent beam electron diffraction.

Any feedback on pros and cons of various software packages would be most appreciated. Cheers,

Mark Blackford
Materials Division, ANSTO
PMB 1,
Menai, N.S.W., 2234
Australia

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the official views of ANSTO from which this message was conveyed.



From daemon Fri Oct 25 05:55:58 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 25 Oct 2002 11:45:04 +0100 (GMT Daylight Time)
Subject: Staining Spurr's Resin

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I would like to improve my staining protocol for Spurr's
resin sections. Our sections tend to be low in contrast
even with 1 day old lead citrate.

We use:
10min uranyl acetate (saturated aqueous) (up to a year old
from a brown bottle stored in the lab)
30min Reynold's lead citrate (up to 2 months old from a
volumentric flask stored in the fridge)

I would be grateful if others could post protocols that
work well.

Dave

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Oct 25 07:15:13 2002



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Fri, 25 Oct 2002 09:07:45 -0300
Subject: European vendors - thanks

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Thanks to everyone who responded regarding European EM
supply vendors. I certainly go a statistically valid sample!

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Fri Oct 25 08:07:47 2002



From: Paul Voyles :      pvoyles-at-bell-labs.com
Date: Fri, 25 Oct 2002 10:13:02 -0400
Subject: Re: electron diffraction simulation software

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Ladd Research, and I assume any of the other supply houses in the US, can
help you with either shipping to Europe or hook you up with and of our
agents in Europe.

Ours are listed on our website at http://www.laddresearch.com

JD Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "James M. Ehrman" {jehrman-at-mta.ca}
To: "Microscopy Listserv" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, October 24, 2002 12:55 PM


At 02:26 PM 10/25/2002 +1000, you wrote:
} I would like to know what software people are using for simulating
} dynamical and kinematical electron diffraction patterns.

I've been very happy with the plane wave multislice implementation by E. J.
Kirkland. It does full dynamical calculations for plane wave and
convergent beam diffraction and HRTEM imaging. The code is efficient -
calculating for 500 A of Si with 1024x1024 beams takes ~13 minutes on my 1
GHz laptoip, but it doesn't come with a snazzy GUI. It's a set of command
line programs.

The best part is you get the whole deal for the cost of Kirkland's book,
"Advanced Computing in Electron Microscopy" Plenum 1998, ISBN
0-306-45936-1. The book comes with a CD the programs and full source code.



Best wishes,
Paul

Paul Voyles
Post-doctoral MTS
Bell Laboratories
700 Mountain Ave, Rm 1D-437
Murray Hill, NJ 07974-0636
voice: (908) 582-4399
fax: (908) 582-4868



From daemon Fri Oct 25 10:26:35 2002



From: khara scott :      kharascott-at-yahoo.com
Date: Fri, 25 Oct 2002 08:17:34 -0700 (PDT)
Subject: SEM Prep Procedures

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Hi listservers:

I am trying to collect pollen on a coverslip and view
under the SEM. I am kind of a beginner. I was
wondering if anyone knew of a procedure to do this
without using the critical point dryer. I've heard
that Hexamethyldisilazine works well. I'm just not
sure about the procedure. Please help.

Thanks,
Khara Scott
EM Technologist
CSU


__________________________________________________
Do you Yahoo!?
Y! Web Hosting - Let the expert host your web site
http://webhosting.yahoo.com/


From daemon Fri Oct 25 10:55:05 2002



From: Wiggins, Winston :      Winston.Wiggins-at-carolinashealthcare.org
Date: Fri, 25 Oct 2002 11:46:29 -0400
Subject: Used UA and OsO4 - Disposal

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Dear Mr. Wiggins,
Salam on you as well. It was nice to hear from somebody ex-desert storm.
Those were tense times. You were far away from the action; for I was here in
Riyadh! In fact, one of the scuds landed just half a mile from where I lived
then. Out of Saudi Arabia and the arab world, the nearest that came to
"shawarma" was the Donner kababs so prevalent in the England (Oxford, Leeds,
Sheffield, Manchester, etc.,). In our private thoughts and senses, we all
seem to be the same. Humans sharing the concerns of other humans. Then, why
this misunderstandings and hatred between nations and people.
Great advise, I shall put that all into practice. I did receive some
nice suggestions from around the world; but yours is the one applicable to
me here in the deep desert.

----- Original Message -----
From: Wiggins, Winston
{mailto:Winston.Wiggins-at-carolinashealthcare.org}
To: 'mohammed y abdulrawoof / f40z006 75760'
{mailto:mdyousuf-at-ksu.edu.sa}
Sent: Wednesday, October 23, 2002 6:01 PM
Subject: RE: Used UA and OsO4 - Disposal



Salaam-u-alaikum Mohammad,
You don't know me but I worked at the King Fahd Medical Center at
King Abdulazziz University in Jeddah, back in 1990-1992. That was before,
during, and after the Desert Storm war. I was the Technical Director in the
Electron Microscopy Lab there. I see that you're in Riyadh at the King Saud
University? I simply wanted to say "Salaam," and that I relished the time I
spent in the Kingdom. It changed my life and gave me a sensitivity toward
the Middle East, Arabs, and Muslims all. I would love to return some day.
Besides, I simply cannot find a decent Saudi-style chicken shawarma anywhere
in this country!! :-)

Now, to your question. My suggestions are based on my experience in
Jeddah, so forgive me if I am being too simplistic.

The used UA can be removed from the hood in its plastic container
and poured in the sand, if you're talking primitive disposal, and I'm sure
you are. It's depleted and is therefore fairly harmless. In the States, a
lot of labs simply pour it down the drain, but only in small amounts with a
lot of water flowing with it. I would find a large open sandy place away
from water or any apparent life, animal or vegetable. I would also make
sure to spread it around and not just pour it out in one spot, then shovel
more sand over it. Halas for the UA.

For the used osmium tetroxide. I would first take it out of the
plastic and put it in a glass container, even if you keep it in the hood.
Plastic is permeable to osmium and you can see that happening when the
plastic turns black. Then, unless you know for sure that it has been
neutralized, I would treat it with NaOH or corn oil in order to make sure it
is neutralized. We use corn oil to neutralize our used osmium because it's
fast and easy. The ratio of corn oil to used osmium is 2:1. So fill a
glass container two-thirds full with corn oil and then fill it up with the
used osmium. You'll probably have to use several containers. We use 1
gallon or 4 liter size glass jugs. After a time, check to determine if
neutralization has occurred by placing a plain piece of white paper on the
opening of the glass container and leaving it for 24 or more hours. If it
hasn't turned black, it's neutralized. If it is black, dilute the mixture
with more corn oil and do the test until the paper remains white. Once it's
neutralized, cap it, then seal that with sealing tape. Put each glass
container in a cardboard, wooden, or metal box with padding to keep the
glass from cracking or breaking during handling and head for the desert or
an incinerator should you have access to one. Aramco should have plenty of
incinerators if you can connect with someone there. If you opt for the
desert, bury the containers in different places in order to spread them
around. I would not leave the containers exposed or pour them out at all.
In'shallah, that should be halas for the used osmium.

N.B. All the handling with the osmium should be done with the proper
personal protective equipment; gloves, lab coat, mask or face shield,
whatever you have available.

And that, my friend, is the end of my dissertation on toxic waste
disposal! If I can be of any more assistance, please do not hesitate to let
me know. If I have not been of service, please let me know that too!

Ma' a-salaama,
Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, Supervisor
Cannon Electron Microscopy Lab
Carolinas Medical Center
P.O. Box 32861; Charlotte, NC 28232-2861
Ship to: 1000 Blythe Blvd; Charlotte, NC 28203
Ofc:704-355-1267 ; Lab:704-355-7220 ; Fax 704-355-0589
E-mail: Winston.Wiggins-at-CarolinasHealthCare.org
{mailto:WWiggins-at-Carolinas.org}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

-----Original Message-----
From: mohammed y abdulrawoof / f40z006 75760
[SMTP:mdyousuf-at-KSU.EDU.SA]
Sent: Tuesday, October 22, 2002 6:44 PM
To: microscopy-at-microscopy.com
Subject: Used UA and OsO4 - Disposal


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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-----------------------------------------------------------------------.

Dear All,
A bulk of used UA (in ethanol) and Osmium tetraoxide has
been
left-over in plastic containers (2 liter bottles; 4 each) at my new
lab.At this place, I do not find any organized disposal system. I
believe, because of this factor,my previous collegues might have
thought
it safe to leave things as such.
The question is; how to get this off my fume-hood, safely,
and
to be seen off into the desert after some self disposal treatment.
Thanks in advance

M. Yousuf Abdul-Rawoof
Riyadh, Saudi Arabia.

mdyousuf-at-ksu.edu.sa



***********************************************************************
This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you.



From daemon Fri Oct 25 11:04:56 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Fri, 25 Oct 2002 10:57:20 -0500
Subject: RE: 35 vs 45 degree diamond knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


35 degree knife will should produce less compression
artifacts in sections. It often recommended for preparation of
the thin sections for work at high magnifications, but in my
opinion in biological applications resolution more limited by
staining techniques than by artifacts from cutting. The knife
sometimes could be useful in cutting of hard materials which
experience really high compression when cut with 55 or 45
degrees knifes. More often it is used for cryo sectioning.
I would not use 35 degree knife for any work that could be
done with 45 or even 55 (for hard tissues or materials) knife,
because it needs to be resharpen more often (quite expensive
procedure) and it could be easily damaged.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Phillips, Thomas E.
} Sent: Thursday, October 24, 2002 3:22 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: 35 vs 45 degree diamond knives
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Any opinions out there on the advantages and disadvantages of a 35
} degree diamond knife compared to a 45 degree one? 35's are supposed
} to cause less compression - is that found in the real world? TIA, tom
}
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}
}


From daemon Fri Oct 25 11:12:26 2002



From: Brett Presley :      presley-at-eyes.uab.edu
Date: Fri, 25 Oct 2002 11:05:06 -0500
Subject: TEM of neutral lipids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a good stain for neutral lipids for TEM? We are
isolating drusen from Bruch's membrane within the human eye and
attempting to capture images of their contents. We have used the
conventional 2 method using 1% Os04 and 0.125% Potassium Ferricyanide
in 1M Phospate buffer. The results were not so great! We have also
used the OTAP protocol consisting of 1% OsO4 in Na Cacodylate buff
followed by 1% tannic acid and 1% paraphenylenediamine. The results
here were a little better ! Any input would be greatly appreciated.
Thanks in advance.
Brett



From daemon Fri Oct 25 12:55:13 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 25 Oct 2002 13:50:27 -0400
Subject: Re: Staining Spurr's Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


First, I would suggest thicker sections. More tissue yeilds more contrast. I
have found that sections that are pale gold after flattening are plenty thin
for most EM work. Almost everyone I have encountered with 'poor contrast' is
looking a silver or grey sections.

Second, alcoholic UA (half-saturated) penetrates better than aqueous UA.

I have been told, but have not verified, that too long in Pb is just as bad
as too little time. 30 minutes seems very long to me, I usually use 5-10
min.

Tissue that is not well osmicated does not pick up heavy metal stains well.


"Patton, David" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I would like to improve my staining protocol for Spurr's
} resin sections. Our sections tend to be low in contrast
} even with 1 day old lead citrate.
}
} We use:
} 10min uranyl acetate (saturated aqueous) (up to a year old
} from a brown bottle stored in the lab)
} 30min Reynold's lead citrate (up to 2 months old from a
} volumentric flask stored in the fridge)
}
} I would be grateful if others could post protocols that
} work well.
}
} Dave
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Oct 25 14:46:06 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 25 Oct 2002 14:37:28 -0500
Subject: Re: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lee,
Yeast, unless you are working with protoplasts, have a very hard cell
wall. I would suggest using a half strength Karnovsky's fix for the basic
fixation (i.e. 2% formalin (PAF) + 2.5% glutaraldehyde in either phosphate
or cacodylate buffer followed by 2% OsO4. Also you will find that osmotic
balance is very important. Try adding about .5M sorbitol. If balance is
off you will most likely have the yeast cell membrane shrinking away from
the cell wall.
Temperature is not critical so fixation can be carried out at room temp.
if desired. You may want to resuspend the yeast in the fix to make sure you
have good fixation throughout. This means that you will be spinning for
fixation and wash steps. If the final pellet does not hold together well you
will have to pellet in low temperature agarose. Then proceed to cut the
resulting pellet into small pieces and continue on with your dehydration.

Dehydrate in either ETON..propylene oxide or acetone series as routinely
done. Infiltrate with Spurr's resin rather than an EPON generic. Don't rush
your infiltration. Use a series of 3:1, 1:1, 1:3, and 100% Spurrs
(dehydrant:resin) over a minimum of 24 hrs...more if time permits.

Contact me if you need more details.
Debby


Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907

On 10/24/02 4:59 PM, "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
} I have just gotten a request to run some samples of yeast (Candida
} sp) up for TEM. Normally I deal with tissues and (eukaryote) cell
} cultures. Can anyone out there give me a few hints about how to deal
} with these wee little beasties, that might differ from a "standard"
} protocol?
} Thanks in advance,
} Lee



From daemon Fri Oct 25 14:46:07 2002



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 25 Oct 2002 09:36:48 -1000 (HST)
Subject: Re: Staining Spurr's Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi-

} I have been told, but have not verified, that too long in Pb is just as bad
} as too little time. 30 minutes seems very long to me, I usually use 5-10
} min.

I can confirm this. In fact, I keep it to three minutes or less these
days. Any longer and you can begin to see bleaching. The higher the pH the
worse the bleaching seems to be.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Fri Oct 25 16:06:17 2002



From: sghoshro-at-NMSU.Edu
Date: Fri, 25 Oct 2002 14:55:20 -0600 (MDT)
Subject: User's committee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

I need some help here. I need to find out what role University wide EM
Facility User's Committee normally should play. We have an issue here
about whether User's Committee should get involved in personnel matter of
the lab (lab staff hiring etc) or they should strictly work as an advisory
committee for a core EM facility.

Are they be the formal supervisors for the lab personnel or it is someone
else's job in the upper administration (e.g.vice president of research)
with some feedback from the user's committee?

Is it good to have a term limit for the user's committee members or in
other way, if some of the existing members are no longer regular users
then should they step down ?

We are in the process of defining the role of user's committee and I will
very much appreciate everyone's input in this matter.

Thanks in advance,

Soumitra

*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty Member, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml



From daemon Fri Oct 25 16:15:32 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Fri, 25 Oct 2002 16:08:28 -0500
Subject: RE: SEM Prep Procedures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The easy (and often sufficient) way to observe pollen is
to place it on SEM stub with double stick carbon tape and
put it in a desecrator for 24 hours. Then, after coating
it in sputter coater, specimens are ready for observation.
Some drying artifacts will occur.

The "proper" way is to dehydrate in graded ethanol (25%, 50%,
75%, 90%, 100%) and in 50-50 ethanol-HMDS (Hexamethyldisilazine)
and in pure HMDS, 5-10 min in each of them. The main difficulty
is separation of pollen from liquid. It can be done with
centrifuge, filter paper or even eyelash, the same which is
used for manipulating with TEM thin sections.

Good luck,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



}
} Hi listservers:
}
} I am trying to collect pollen on a coverslip and view
} under the SEM. I am kind of a beginner. I was
} wondering if anyone knew of a procedure to do this
} without using the critical point dryer. I've heard
} that Hexamethyldisilazine works well. I'm just not
} sure about the procedure. Please help.
}
} Thanks,
} Khara Scott
} EM Technologist
} CSU
}
}
} __________________________________________________
} Do you Yahoo!?
} Y! Web Hosting - Let the expert host your web site
} http://webhosting.yahoo.com/
}
}


From daemon Fri Oct 25 17:43:54 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Fri, 25 Oct 2002 18:32:26 -0400
Subject: Re: SEM/CL lifetime measurements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mark,
For a reasonably fast beam blanker, contact Earl Weltmer of Scanservice
Corp.(above addresss). I don't have the specs in front of me but I
believe he specs the transition at 100nS and in most systems it is
considerably faster, but I don't want to quote on that without
conferring with Earl. This is an electrostatic system as opposed to
electromagnetic, which JEOL provides (and is no where near as fast -
about 1uS).

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Mark Johnson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Fri Oct 25 21:35:45 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 25 Oct 2002 19:31:32 -0700
Subject: spam control

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is a free spam control program that works on
various versions of Windows. Sorry, no Mac.

The program is called MailWasher http://www.mailwasher.net

It does POP3 and APOP screening of messages for spam.
It also includes a comprehensive set of blacklisted domains
and addresses, allows qualifying friends from being kicked out,
and supports stock and custom filters.

The author is from and in New Zealand and the program is
IMHO, very good. It is perhaps worth a try to see if it would
help you sort out (dump) spam.

Before you engage it, do read the FAQ. If you find some
aspect not to your liking or compatible with your e-mail
situation, don't install it. It will delete from your server
what it is told is spam. You will have to teach it what is
not spam if it thinks otherwise. But the lists of blacklisted
domains is very powerful. You will most likely see a
huge decrease in spam.

I personally get about 95 messages per day, out of which
only 5 are valid. MailWasher gets all but a couple of the
trash messages.

gary g.



From daemon Fri Oct 25 23:54:24 2002



From: Zhaojie Zhang :      ZZhang-at-uwyo.edu
Date: Fri, 25 Oct 2002 22:45:08 -0600
Subject: Re:TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lee:

Robin Wright has a very nice review about TEM of yeast (Saccharomyces,
not Candida). I found it very helpful.

Robin Wright (2000) Transmission Electron Microscopy of Yeast.
Microscopy Research and Technique 51(6): 496-510


Zhaojie Zhang
Director, Microscopy Facility
Department of Zoology and Physiology
University of Wyoming
Laramie, WY 82071


-----Original Message-----
} From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
Sent: Thursday, October 24, 2002 3:59 PM
To: Microscopy-at-sparc5.microscopy.com


Hi All,
I have just gotten a request to run some samples of yeast (Candida
sp) up for TEM. Normally I deal with tissues and (eukaryote) cell
cultures. Can anyone out there give me a few hints about how to deal
with these wee little beasties, that might differ from a "standard"
protocol?
Thanks in advance,
Lee
--
Lee Cohen-Gould
Electron & Optical Microscopy Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207



From daemon Sun Oct 27 11:33:40 2002



From: akc-at-umich.edu
Date: Sun, 27 Oct 2002 12:11:57 -0500
Subject: Re:TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lee,

I haven't done EM of yeast, but am aware that the ultrastructure has
generally been disappointing. The cell wall seems to limits the
penetration of fixative and other solutions, leading to suboptimal
preservation. An article appeared recently that suggested a solution to
this problem. After the initial 2% glutaraldehyde fixation for 30 min (0.1
M Na-cacodylate buffer, 4 degrees C, 1 mM CaCl2) and buffer wash, there is
an enzymatic digestion step (Zymolyase 20T) to remove the cell wall. This
is then followed by postfixation in OsO4 (0.5% and 0.8% potassium
ferrocyanide in distilled water, 2 times for 5 min at 4 degrees C),
embedding in agarose, en bloc staining with uranyl acetate, dehydration and
embedment in Epon. The ultrastructure looks very good, with
clearly-defined, well-stained membranes.

The reference is: Christoph Bauer, Volker Herzog and Matthias F. Bauer,
2001, "Improved technique for electron microscope visualization of yeast
membrane structure," Microscopy and Microanalysis 7:530-534.

As you are probably aware, Microscopy and Microanalysis is the official
journal of the Microscopy Society of America (formerly the EMSA), which
sponsors this list server. If you want a reprint, you can contact the
corresponding author, Matthias F. Bauer, at Howard Hughes Medical
Institute, Department of Molecular Genetics and Cell Biology, University of
Chicago, 5841 S. Maryland, Chicago, IL 60637. The article also cites the
two main previous references on yeast EM: Byers B, Goetsch L (1991) Methods
Enzymol 194:602-608. Wright R (2000) Microsc Res Tech 51:496-510.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Thursday, October 24, 2002 5:59 PM -0400 Leona Cohen-Gould
{lcgould-at-med.cornell.edu} wrote:

} Hi All,
} I have just gotten a request to run some samples of yeast (Candida sp) up
} for TEM. Normally I deal with tissues and (eukaryote) cell cultures.
} Can anyone out there give me a few hints about how to deal with these wee
} little beasties, that might differ from a "standard" protocol? Thanks in
} advance,
} Lee
} --
} Lee Cohen-Gould
} Electron & Optical Microscopy Facilities
} Weill Medical College of Cornell U.
} (212)746-6146
} Rms A-105, LC-207








From daemon Sun Oct 27 21:46:56 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Mon, 28 Oct 2002 14:39:50 +1100
Subject: Re: Fixation of mitochondria (Muscle TEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Re. morphology of mitochondria, as noted by others below, it's quite
variable at TEM level, and fixation will affect it substantially. Number,
shape, size and ultrastructure of mitochondria in a particular cell type
will vary depending on stage in cell cycle, cell physiology - whether
quiescent or not, activated by stimulus or not, etc, etc. Also varies with
cell type. For good ultrastructural preservation, I would use rapid
freezing and freeze substitution in preference to chemical fixation.
What do you mean by "pores" in mitochondria?
cheers,
Roseamry
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dr Rosemary White rosemary.white-at-csiro.au
Microscopy Centre fax 61- 2 6246 5000
CSIRO Plant Industry ph. 61- 2 6246 5475 or
GPO Box 1600 mob. 61- 0402 835 973
Canberra, ACT 2601, Australia




From daemon Mon Oct 28 06:57:32 2002



From: Julian Martinez Fernandez :      julianmartinezfernandez-at-hotmail.com
Date: Mon, 28 Oct 2002 13:41:51 +0100
Subject: Thermal or cold FE SEM electron gun?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleges:

We are about to get an Field Emission SEM. We are debating between a
thermal or a cold electron gun. Could you give me some input of your
personal experience?

Many thanks,
Julian

____________________
Julián Martínez Fernández
Profesor Titular de Universidad
Dpto. de Física de la Materia Condensada
Facultad de Física
Universidad de Sevilla
Apdo. 1065 41080 Sevilla
Spain

Tel. int+34 954556956
Fax int+34 954612097
e-mail martinez-at-us.es


From daemon Mon Oct 28 07:34:31 2002



From: Ssjh1818-at-aol.com
Date: Mon, 28 Oct 2002 08:22:53 -0500
Subject: Re: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


usualy i tell people try pub med first. it is much more satisfying to obtain the info yourself than have it handed to you. thats the way i was taught, but alas those days are gone. i guess no one knows how to perform lit searches anymore. here is the result of a 2 min search on pub med using the keywords tem and yeast:
Microsc Res Tech 2000 Dec 15;51(6):496-510 Related Articles, Links


Transmission electron microscopy of yeast.

Wright R.

University of Washington, Department of Zoology, Seattle, WA 98195-1800, USA. wrightr-at-u.washington.edu

The challenges of sample preparation can limit a researcher's selection of transmission electron microcopy (TEM) for analysis of yeast. However, with the exception of thin sectioning, preparation of well-fixed and infiltrated samples of yeast cells is achievable by any reasonably equipped laboratory. This review presents a general overview of TEM sample preparation methods and detailed protocols for chemical fixation of yeast for ultrastructural analysis and immunolabeling. For ultrastructural analysis, the most commonly used chemical fixation involves treatment with glutaraldehyde followed by either potassium permanganate or osmium. Prior to osmium postfixation, the cell wall must be enzymatically digested to allow optimal fixation and embedding. Freeze substitution methods continue to provide the highest quality of fixation, but equipment needed for these protocols is not generally available to many labs. The low viscosity of Spurr's resin makes it the resin of choice for ultrastructure studies. Immunoelectron microscopy has enjoyed great success in analysis of yeast molecular organization. For immunoelectron microscopy, glutaraldehyde/formaldehyde-fixed cells are embedded in LR White resin. The thin sections are then treated in much the same way as an immunoblot: following blocking, they are incubated in primary antiserum, washed, and then incubated in gold-labeled secondary antiserum. Copyright 2000 Wiley-Liss, Inc.



From daemon Mon Oct 28 07:48:50 2002



From: Ssjh1818-at-aol.com
Date: Mon, 28 Oct 2002 08:39:10 -0500
Subject: Re: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


usualy i tell people try pub med first. it is much more satisfying to obtain the info yourself than have it handed to you. thats the way i was taught, but alas those days are gone. i guess no one knows how to perform lit searches anymore. here is the result of a 2 min search on pub med using the keywords tem and yeast:
Microsc Res Tech 2000 Dec 15;51(6):496-510 Related Articles, Links


Transmission electron microscopy of yeast.

Wright R.

University of Washington, Department of Zoology, Seattle, WA 98195-1800, USA. wrightr-at-u.washington.edu

The challenges of sample preparation can limit a researcher's selection of transmission electron microcopy (TEM) for analysis of yeast. However, with the exception of thin sectioning, preparation of well-fixed and infiltrated samples of yeast cells is achievable by any reasonably equipped laboratory. This review presents a general overview of TEM sample preparation methods and detailed protocols for chemical fixation of yeast for ultrastructural analysis and immunolabeling. For ultrastructural analysis, the most commonly used chemical fixation involves treatment with glutaraldehyde followed by either potassium permanganate or osmium. Prior to osmium postfixation, the cell wall must be enzymatically digested to allow optimal fixation and embedding. Freeze substitution methods continue to provide the highest quality of fixation, but equipment needed for these protocols is not generally available to many labs. The low viscosity of Spurr's resin makes it the resin of choice for ultrastructure studies. Immunoelectron microscopy has enjoyed great success in analysis of yeast molecular organization. For immunoelectron microscopy, glutaraldehyde/formaldehyde-fixed cells are embedded in LR White resin. The thin sections are then treated in much the same way as an immunoblot: following blocking, they are incubated in primary antiserum, washed, and then incubated in gold-labeled secondary antiserum. Copyright 2000 Wiley-Liss, Inc.





From daemon Mon Oct 28 08:51:43 2002



From: Ann-Fook Yang :      yanga-at-agr.gc.ca
Date: Mon, 28 Oct 2002 09:41:49 -0500
Subject: Re: User's committee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a users' committee. The members are usually regular users and representing a group of people working in the same field. Their primary function is to involve in a major equipment selection process, e.g. find out the requirements for their group and to convince upper management that a new equipment is required. They do not deal with day to day function of the EM lab, which is the job of a supervisor. They can, of course, suggest what to do to the supervisor; but they should not have power over the supervisor. If they do, they would drive the supervisor and technicians crazy! -- too many heads with different ideas.



AnnFook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Room 2091, Bldg. 20,
Central Experimental Farm,
Ottawa, Ontario
Canada K1A 0C6

Tel: 1-613-759-1638
Fax: 1-613-759-1701

e-mail: yanga-at-em.agr.ca

} } } {"sghoshro-at-NMSU.Edu"-at-sparc5.microscopy.com} 10/25/02 04:55PM } } }
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Dear Microscopists,

I need some help here. I need to find out what role University wide EM
Facility User's Committee normally should play. We have an issue here
about whether User's Committee should get involved in personnel matter of
the lab (lab staff hiring etc) or they should strictly work as an advisory
committee for a core EM facility.

Are they be the formal supervisors for the lab personnel or it is someone
else's job in the upper administration (e.g.vice president of research)
with some feedback from the user's committee?

Is it good to have a term limit for the user's committee members or in
other way, if some of the existing members are no longer regular users
then should they step down ?

We are in the process of defining the role of user's committee and I will
very much appreciate everyone's input in this matter.

Thanks in advance,

Soumitra

*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty Member, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml





From daemon Mon Oct 28 11:06:26 2002



From: Mathes, David (TX95) :      David.Mathes-at-honeywell.com
Date: Mon, 28 Oct 2002 10:54:28 -0600
Subject: Selective metal etch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I would like to be able to selectively remove a gold contact from an
AlGaAs device for some SEM analysis. Can anyone direct me to the proper
etch? The only etches I'm familiar with also etch AlGaAs.

Thanks
Dave



__________________________________
David Mathes, Ph.D.
Principle Failure Analysis Engineer

VCSEL Optical Products
Honeywell Inc.
830 E. Arapaho Rd., Richardson, TX 75081
Phone: 972-470-4683
Fax: 972-470-4504
e-mail: david.mathes-at-honeywell.com



From daemon Mon Oct 28 11:44:53 2002



From: Dan Bumbarger :      danbu-at-citrus.ucr.edu
Date: Mon, 28 Oct 2002 09:29:07 -0800
Subject: environmental SEM

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I am working with some fairly delicate nematode material which is
collapsing quite a bit when preparing for SEM. I would like to try an
environmental SEM, but there are no facilities for this work at my
University. I am aware that there is a microscope at UC Berkeley, but I
was wondering if anyone is aware of facilities that might be located in
southern california that would be easier for me to access.
--------------------------------------------------------
Dan Bumbarger
Graduate Student
Departments of Biology and Nematology
University of California
Riverside, CA 92521
(909)787-3922
--------------------------------------------------------

“With a little more deliberation in choice of pursuits, all men would
perhaps become essentially students and observers, for certainly their
nature and destiny are interesting to all alike. In accumulating
property for ourselves or our posterity, in founding a family or a
state, or acquiring fame even, we are mortal; but in dealing with truth
we are immortal, and need fear no change nor accident.”
-H.D. Thoreau, “Walden”



From daemon Mon Oct 28 13:19:33 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 28 Oct 2002 14:08:06 -0500
Subject: Re:TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
Thanks for (most)all of your replies to my inquiry. There was a
general consensus about post-fixing in osmium & K-ferricyanide (which
I use most of the time anyway), following up with K-permanganate and
embedding in Spurrs.
I will check the various references cited and this time I will keep
my file where I can find it again!

Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Oct 28 14:10:04 2002



From: Wiggins, Winston :      Winston.Wiggins-at-carolinashealthcare.org
Date: Mon, 28 Oct 2002 15:01:56 -0500
Subject: RE: Staining Spurr's Resin

Contents Retrieved from Microscopy Listserver Archives
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David,
We've had the same problem using Spurr's. In my efforts to remedy this
problem, I learned that Spurr's is inherently low "contrast-y", no matter
what you do to increase contrast!
Generally, according to Hayat (Principles and Techniques of Electron
Microscopy, Biological Applications, 3rd ed., by M.A. Hayat), "...it is
advisable to keep the staining time as short as possible, for prolonged
exposure will cause extraction of cellular materials or even de-staining.
Most lead salts stain most effectively in a few minutes. On the average, a
range of 2-15 minutes is adequate." He also states, "Small apertures and
low voltages give greater contrast." He offers a ton of other factors
affecting image contrast.
We use Sato's triple lead stain which works fairly well. To make 50 ml of
staining solution, mix 0.5g lead acetate, 0.5g lead citrate, 0.5g lead
nitrate, 1.0g sodium citrate, and 41.0ml distilled, boiled,
cooled-to-room-temperature-in-a-capped-container water, for 1-2 hours on a
stir plate. Then add 9ml of fresh 4% sodium hydroxide (easily made with 1.0g
NaOH pellets + 25ml of that distilled, boiled,
cooled-to-room-temperature-in-a-capped-container water.)
I stain with saturated, aqueous uranyl acetate for 10 minutes and
then Sato's for 5 minutes. Try that and adjust from there, but remember,
Spurr's is inherently low "contrasty."
Good luck!
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, Supervisor
Cannon Electron Microscopy Lab
Carolinas Medical Center
P.O. Box 32861; Charlotte, NC 28232-2861
Ship to: 1000 Blythe Blvd; Charlotte, NC 28203
Ofc:704-355-1267 ; Lab:704-355-7220 ; Fax 704-355-0589
E-mail: Winston.Wiggins-at-CarolinasHealthCare.org
{mailto:WWiggins-at-Carolinas.org}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


-----Original Message-----
From: Patton, David [SMTP:David.Patton-at-uwe.ac.uk]
Sent: Friday, October 25, 2002 6:45 AM
To: microscopy-at-sparc5.microscopy.com
Subject: Staining Spurr's Resin


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I would like to improve my staining protocol for Spurr's
resin sections. Our sections tend to be low in contrast
even with 1 day old lead citrate.

We use:
10min uranyl acetate (saturated aqueous) (up to a year old
from a brown bottle stored in the lab)
30min Reynold's lead citrate (up to 2 months old from a
volumentric flask stored in the fridge)

I would be grateful if others could post protocols that
work well.

Dave

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




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From daemon Mon Oct 28 14:12:13 2002



From: Thearith H. Ung :      tung-at-qdots.com
Date: Mon, 28 Oct 2002 11:58:06 -0800
Subject: RE:Low contrast TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi everyone,

I am looking for TEM grids that have extremely low contrast for imaging
semiconductor 1-2 nm nanoparticles. I would really appreciate it if you cab
contact me if you know where I can purchase such grids. Thank you.

Thearith
_________________

Thearith Ung

Quantum Dot Corporation
26118 Research Road
Hayward, CA 94545, USA
Tel: 510-887-8775 (Ext 4125)
Fax: 510-783-9729
Website: http://www.qdots.com



From daemon Mon Oct 28 14:14:09 2002



From: Doug Keene :      drk-at-shcc.org
Date: Mon, 28 Oct 2002 09:21:04 -0800 (Pacific Standard Time)
Subject: Staining Spurr's Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello David,

We typically use Spurrs resin and stain sections for 15
minutes in (2% uranyl acetate dissolved in 50% EtOH)
followed by 60 seconds in Reynold's lead citrate. Grids are
washed for about 5 minutes in deionized/distilled water
after each staining step. This results in excellent
contrast for silver or gold sections. When staining
thicker sections (quarter to half micron) we increase our
time in UA to one hour and in lead to 15 minutes.

Good luck,

Doug


----------------------
Douglas R. Keene
Micro-Imaging Center
Shriners Hospital for Children
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97239
Phone: 503-221-3434
FAX: 503-412-6894



From daemon Mon Oct 28 14:29:33 2002



From: Eric Steel :      eric.steel-at-nist.gov
Date: Mon, 28 Oct 2002 15:15:10 -0500
Subject: Post Doc Openings

Contents Retrieved from Microscopy Listserver Archives
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The National Institute of Standards & Technology (NIST) has many Post
Doctoral Positions open. These are offered competitively through the
National Research Council (NRC). Within our research areas we have many
possible openings described at the web site listed below. We have about
forty full time scientists specializing in microscopy and microanalysis
measurement methods in our extensive facilities including:

SEMs, AEMs, Auger probes, MicroXRD/XRF, Synchrotron beam-line with grazing
incidence XPS, Chemometrics, NSOM and other scanned probes, SIMS/TOF-SIMS,
Isotopic analysis, MicroRaman/IR, etc.

We develop and test new and improved measurement methods and we apply these
methods to challenging analytical problems in a variety of areas including
semiconductor and optoelectronic technology, particle characterization,
materials science, environmental and earth science, etc. If you have
research ideas that would be related to the analytical approaches below and
are looking for a Post Doc opportunity. The NIST/NRC program offers a
two-year Post Doc at an annual salary of approximately $55,700 with an
additional $5,500 for research expenses. The applications are due to the
NRC by Jan 15, 2003. This includes a brief proposal and several
recommendations.

A candidate must be a U.S. citizen and start work (with their PhD in hand)
at NIST between July 1, 2003 and Feb 1, 2004. So, this is the perfect
opportunity for those that are graduating this winter through next fall and
others that have received their degree within the last five years. Please
note that NIST/NRC only competes Post Doc positions once a year, unlike
some other institutions.

For more details see:
http://www.cstl.nist.gov/div837/Division/opportunities/PostDoctoral.htm

If interested please contact:

Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-417-1321
100 Bureau Drive, Stop 8371
Gaithersburg, MD 20899-8371
http://www.nist.gov/micro



From daemon Mon Oct 28 14:32:43 2002



From: jerry smith :      jsmit51-at-tampabay.rr.com (by way of
Date: Mon, 28 Oct 2002 14:26:39 -0600
Subject: RBSE PARTS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


DOES ANYONE OUT THERE , KNOW WHERE WE
CAN GET REPLACEMENT PARTS FOR RBSE
DETECTORS.. THEY USED TO BE ETP-SEMRA, BUT
DO NOT SEEM TO BE LISTED AS SUCH ANYMORE

JERRY


From daemon Mon Oct 28 16:14:16 2002



From: sstouden-at-thelinks.com
Date: Mon, 28 Oct 2002 16:40:11 -0600 (CST)
Subject: Re: Fixation of mitochondria (Muscle TEM)

Contents Retrieved from Microscopy Listserver Archives
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Dear Mr. Patton,
I too have a similar experience (low contrast), while staining Spurr's Resin
embedded sections. There seems to be no improvement even after doing enblock
staining with UA followed again by double staining the grids. I would like
to hear more to this problem from you and other listers around. Please
forword me any advisory mails that you may receive exclusively.
Have a good day.
M. Yousuf Abdul-Rawoof
Riyadh, KSA.
----- Original Message -----
} From: "Patton, David" {David.Patton-at-uwe.ac.uk}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Friday, October 25, 2002 1:45 PM


pores = the intra compartimental openings that filter or respond to
ligands . Most of the pores I am talking about are
genetically constructed in response to metabolic change.
Let me be clear, at this size level I am not sure of anything..

On Mon, 28 Oct 2002, Rosemary White wrote:

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} -----------------------------------------------------------------------.
}
}
} Re. morphology of mitochondria, as noted by others below, it's quite
} variable at TEM level, and fixation will affect it substantially. Number,
} shape, size and ultrastructure of mitochondria in a particular cell type
} will vary depending on stage in cell cycle, cell physiology - whether
} quiescent or not, activated by stimulus or not, etc, etc. Also varies with
} cell type. For good ultrastructural preservation, I would use rapid
} freezing and freeze substitution in preference to chemical fixation.
} What do you mean by "pores" in mitochondria?
} cheers,
} Roseamry
} } ------------------------------------------------------------------------
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} }
} }
} }
} } pardon me, but i would like to ask very simple question, which I am sure
} } everyone else on this list but me is able to answer,
} }
} } what is the morphology of a standard mitochrondium:
} }
} } how many standard mitochrondia structures are there?
} } do structures appear and disappear within the same mitochrondrium
} } Does cell type of origin change the standard of the structure?
} } Is there a mitochrondria database somewhere?
} } what about pore type intra mitochondrial and intercompartmental?
} } pore distribution function and density seems to be the big determinate of
} } mitochrondiral differentiation and function.
} } what are they.
} } the big structure we all know, it the little ones, that I am talking
} } about, some that are sometimes there and sometimes not.
} }
} }
} }
} } how are the different structures separately classified in each such
} } mitochrondria?
} } is there a measurement technique to determine exact location and volume of
} } these intra mitochrondrial structures?
} }
} } is there a noticeable by experimental method difference between
} } mitochrondria of one source or the other other.
} }
} } thanks for indulging my ignorance.
} } sterling
} }
} } On Tue, 22 Oct 2002, Sergey Ryazantsev wrote:
} }
} } } ------------------------------------------------------------------------
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} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Mitochondria is very sensitive to so many factors, which we practically
} } } could not count. Oxidative stress is one of the major problem here. "Well
} } } fixed" mitochondria is only "reflection" of the real structure, not real
} } } things itself (as any other chemically fixed structure). I could imagine
} } } the experiment, when we will take/process samples at the very identical
} } } conditions. In this case we will probably see very small mitochondria
} } } structure variation from sample to sample. In practice, there are so many
} } } factors we could not count/reproduce. Therefore we expect to have a
} } } diversity of the structure presentation from the identical organelle,
} } } mitochondria in our case. So, we have a "distribution" of the
} } } structure. If we are studying some "effect on mitochondria" then we have
} } } to compare two "distributions" from control and experiment. If the
} } } "distributions" are not cross overed, we could talk about some
} } } changes. This is simplification of the problem of coarse. In my point of
} } } view, it's relatively simply to compare such "distributions" on the level
} } } of morphological changes and much, much more difficult on quantitative
} } } level (if even possible). In general, the data analysis is not only
} } } scientific issue, it's also ethical issue. I did see people who was
} } } extremely smart manipulating the EM data in favor of their theories...
} } }
} } } As for direct question in original posting, just general recommendations:
} } } take smallest possible piece of tissue, immediately immerse them into the
} } } fixer and cut on smaller pieces in the fixer to 1x1x1 mm with new scalpel,
} } } then cut each piece to 0.5x0.5x0.5 mm, then move tissue in the fresh fixer
} } } and fix 1-2 hours on ice. I usually do everything on ice, but you may try
} } } to do first step (cutting tissue, 1st change of the fixer) at the room temp
} } } to reduce temperature shock (use FRESH fixer for the change!). Personally,
} } } I prefer 1x PBS instead cacodylate and 1.5% GA is enough for such small
} } } pieces. Then I would wash tissue with 1x PBS for 30 min and fix in 1% OSO4
} } } (in PBS or just in water) for 1 h ON ICE. 30 min water wash
} } } thereafter. All solutions should be fresh and made from "EM grade"
} } } chemicals and 18 MOhm "cell culture quality" deionized water. I am using
} } } GA solutions no longer than one hour after preparation if stored on
} } } ice. OSO4 needs to be prepared just before use and stored on ice. Time
} } } between collecting the tissue/animal death and fixation should be 20-30
} } } seconds or SHORTER if we are talking about "good" mitochondria fixation.
} } } The way you kill animal have dramatic effect on mitochondria!
} } } Good luck. Sergey.
} } }
} } }
} } } At 08:30 AM 10/23/02 +1300, you wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Kia Ora
} } } }
} } } } Marcs question about mitochondria is a little timely for us.
} } } }
} } } } In our Unit recently we have been experiencing an increase in the number
} } } } of investigators who are wanting to quantify mitochondria morphology,
} } } } usually after some experimental change.
} } } }
} } } } eg 1, changes in muscle mitochondria in resistance trained athelete's
} } } } (weight lifters) compared to endurance trained athelete's (runners) (Human)
} } } }
} } } } eg 2, changes in brain stem mitochondria after a particular anaesthetic
} } } } and neuro-surgical procedure is used (Rat)
} } } }
} } } } eg 3, mitochondria changes in various tissues of hibernating hedgehog
} } } } compared to non hibernating hedgehog
} } } }
} } } } eg 4, mitochondria changes in fish as they move from sea water 'lifestyle'
} } } } to a fresh water 'lifestyle'.
} } } }
} } } } eg 5. mitchondria changes in cultured cells after biochemically induced.
} } } } apoptosis.
} } } }
} } } } These projects are all looking at morphological changes of the
} } } } mitochondria rather than chnages in the number of mitochondria.
} } } }
} } } } I have some niggly concerns about these projects but I can't really put a
} } } } finger on it. Mitochondria are such sensitive souls that I am not
} } } } entirely convinced that some of the changes being noted are not tissue
} } } } dissection related or fixation related (ie some are perfused, some are
} } } } immersion fixed etc) rather than actually experiment related.
} } } }
} } } } My niggles are not because of something really obvious like an obvious
} } } } osmotic problem, or delays in fixative penetration, or using poor quality
} } } } aldehyde. It is just a gut feeling. Perhaps it is simply different
} } } } 'appearances' of mitochondria in the diferent tissues.
} } } }
} } } } So I don't have a specific question, more I would interested in peoples
} } } } comments on assessing the quality of mitochondria fixation from a
} } } } morphological point of view rather than a technique point of view.
} } } }
} } } } I haven't been able to find any good reference material, apart from that
} } } } which covers technique problems, that has been able to dispel my niggle.
} } } }
} } } } Anyone got any thoughts on precautions to take in preparation when
} } } } specifically focusing on mitochondria morphology ?
} } } }
} } } } Thanks
} } } }
} } } } Allan
} } } }
} } } }
} } } } } ------------------------------------------------------------------------
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} } } } } -----------------------------------------------------------------------.
} } } } }
} } } } }
} } } } } Dear List,
} } } } }
} } } } } I would appreciate recieveing any fixation protocol you know
} } } } } works well for mouse muscle (extensor digitorum longus (EDL)
} } } } } and epitrochlearus (EPI)). We use routinely immersion into
} } } } } 2.5% glutaraldehyde in 0.1M cacodylate pH 7.4, but have had
} } } } } often problems with mitochondrial swelling. Since this project is
} } } } } to quantify mitochondrial density, we do need perfect and
} } } } } reliable fixation of all mitos. Perfusion is not an option. Thanks
} } } } } for any advise on type of fixative, buffer and temperature.
} } } } }
} } } } } Marc
} } } } } --
} } } } } Marc Pypaert
} } } } } Department of Cell Biology,
} } } } } Center for Cell and Molecular Imaging and
} } } } } Ludwig Institute for Cancer Research,
} } } } } Yale University School of Medicine
} } } } } 333 Cedar Street
} } } } } New Haven CT 06520
} } } } } Tel : (203) 785 3681
} } } } } Fax : (203) 785 7446
} } } }
} } } } --
} } } } -------------------------------------------------
} } } } Allan Mitchell
} } } } Technical Manager
} } } } Otago Centre for Electron Microscopy
} } } } C/-Department of Anatomy and Structural Biology
} } } } School of Medical Sciences
} } } } P.O. Box 913
} } } } Dunedin
} } } } New Zealand
} } } }
} } } } Phone (03) 479 5642 or 479 7301
} } } } Fax (03) 479 7254
} } } }
} } } } Unit: http://www.otago.ac.nz/anatomy/emunit/
} } } } Department: http://anatomy.otago.ac.nz/
} } } }
} } } }
} } } }
} } } } "Life is a gift, don't waste it"
} } }
} } }
}
}
} Dr Rosemary White rosemary.white-at-csiro.au
} Microscopy Centre fax 61- 2 6246 5000
} CSIRO Plant Industry ph. 61- 2 6246 5475 or
} GPO Box 1600 mob. 61- 0402 835 973
} Canberra, ACT 2601, Australia
}
}
}



From daemon Mon Oct 28 16:54:01 2002



From: Thearith H. Ung :      tung-at-qdots.com
Date: Mon, 28 Oct 2002 14:39:47 -0800
Subject: Low contrast TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi everyone,

I am looking for TEM grids that have extremely low contrast for imaging
semiconductor 1-2 nm nanoparticles. I would really appreciate it if you can
let me know where I can purchase such grids. Thank you.

Thearith
_________________

Thearith Ung

Quantum Dot Corporation
26118 Research Road
Hayward, CA 94545, USA
Tel: 510-887-8775 (Ext 4125)
Fax: 510-783-9729
Email: tung-at-qdots.com





From daemon Mon Oct 28 17:41:33 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 28 Oct 2002 15:32:45 -0800
Subject: Re: RBSE PARTS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


http://www.etp-usa.com/


At 12:26 PM 10/28/2002, you wrote:
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From daemon Tue Oct 29 00:58:22 2002



From: George.Theodossiou-at-csiro.au
Date: Tue, 29 Oct 2002 17:40:50 +1100
Subject: RBSE PARTS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jerry,

Your after:
Dr Vivian Robinson

244 Canterbury Rd, Canterbury
N.S.W 2193 Australia
Tel +61 (2) 9718 1444
Fax +61 (2) 9718 8222
email: viv-at-etpsemra.com.au

I'm sure he can help.

Regards
George

-----Original Message-----
} From: jerry smith [mailto:jsmit51-at-tampabay.rr.com]
Sent: Tuesday, 29 October 2002 7:27 AM
To: microscopy-at-sparc5.microscopy.com


DOES ANYONE OUT THERE , KNOW WHERE WE
CAN GET REPLACEMENT PARTS FOR RBSE
DETECTORS.. THEY USED TO BE ETP-SEMRA, BUT
DO NOT SEEM TO BE LISTED AS SUCH ANYMORE

JERRY


From daemon Tue Oct 29 07:41:32 2002



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Tue, 29 Oct 2002 08:30:51 -0500
Subject: Re: environmental SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Be aware your sample may still be vacuum liable and susceptible to beam
damage. A SEM with cryo stage would be my first choice

Scott Whittaker
Laboratories of Analytical Biology
Smithsonian Institution
National Museum of Natural History
PO Box 37012 MRC104
Washington DC 20013-7012
202-357-1651


} } } Dan Bumbarger {danbu-at-citrus.ucr.edu} 10/28/02 12:29PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am working with some fairly delicate nematode material which is
collapsing quite a bit when preparing for SEM. I would like to try an
environmental SEM, but there are no facilities for this work at my
University. I am aware that there is a microscope at UC Berkeley, but I
was wondering if anyone is aware of facilities that might be located in
southern california that would be easier for me to access.
--------------------------------------------------------
Dan Bumbarger
Graduate Student
Departments of Biology and Nematology
University of California
Riverside, CA 92521
(909)787-3922
--------------------------------------------------------

"With a little more deliberation in choice of pursuits, all men would
perhaps become essentially students and observers, for certainly their
nature and destiny are interesting to all alike. In accumulating
property for ourselves or our posterity, in founding a family or a
state, or acquiring fame even, we are mortal; but in dealing with truth
we are immortal, and need fear no change nor accident."
-H.D. Thoreau, "Walden"




From daemon Tue Oct 29 08:20:10 2002



From: Beverly Giammara :      giammara-at-bellsouth.net
Date: Tue, 29 Oct 2002 09:20:36 -0500
Subject: SEM/EDAX on Discovery Channel

Contents Retrieved from Microscopy Listserver Archives
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Dear Friends and Colleagues,
It was quite an experience last spring when a film crew
came into the SEM laboratory for the whole day to film
a "re-creation" of the SEM and EDAX used to help analyze
the exhumated remains of Zachary Taylor. Taylor, from Kentucky, was
the 12th President of the US and died in 1850. The Medical Examiner
needed to determine if he had been poisoned with arsenic.
This film-story will be shown Wednesday, October 30th
at 9:00 on the Discovery Channel as "Unsolved History: Forensics
in the White House." Remember that this is Show Biz and also the
night before Halloween!
Kind regards to all. Beverly Giammara


From daemon Tue Oct 29 08:54:12 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 29 Oct 2002 10:30:57 -0500
Subject: Selective metal etch

Contents Retrieved from Microscopy Listserver Archives
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Dave;

Aqueous porassium iodide will do it. However, if there is a fracture in the
AlGaAs surface, it will etch preferentially down a crystal plane. Acton
Technologies in PA makes a "GE-6" gold etchant. http://www.actontech.com/

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Mathes, David (TX95) [mailto:David.Mathes-at-honeywell.com]
Sent: Monday, October 28, 2002 11:54 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Hi,
I would like to be able to selectively remove a gold contact from an
AlGaAs device for some SEM analysis. Can anyone direct me to the proper
etch? The only etches I'm familiar with also etch AlGaAs.

Thanks
Dave



__________________________________
David Mathes, Ph.D.
Principle Failure Analysis Engineer

VCSEL Optical Products
Honeywell Inc.
830 E. Arapaho Rd., Richardson, TX 75081
Phone: 972-470-4683
Fax: 972-470-4504
e-mail: david.mathes-at-honeywell.com



From daemon Tue Oct 29 11:14:36 2002



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Tue, 29 Oct 2002 11:04:18 -0600
Subject: Re: Staining Spurr's Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi David Patton,
I used to stain Spurr's sections with methonalic UA (2-3% UA in 95%
methanol) for only 5 minutes, followed by Reynold's lead for 10-15
minutes. This is pretty extreme (some people use various percentages of
ethanol) but I got lots of contrast. The drawback is that methanol softens
the sections and they detach from the copper grids. To adhere the sections
to the grids (and flatten/stretch them out so they don't wrinkle up in the
methanol), I put the forceps holding the grid with the sections I just
picked up (still with a film of water over them) onto a 60 degree slide
warmer under a big Petri dish with a cotton swab dipped in toluene (or some
other solvent like xylene). The toxic vapors from the toluene soften the
plastic and anneal it to the grid so that the sections stick and don't wash
off during staining.
Nowadays, I routinely use Polybed 812, and get good contrast, because
Spurr's wouldn't work for me when I attempted immunolabeling on thin
sections collected on nickel grids. I still dry all my grids by putting
the forceps still holding the wet sections on a slide warmer, but without
the Petri dish covering and toluene swab.
Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242-1324



From daemon Tue Oct 29 11:16:47 2002



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Tue, 29 Oct 2002 11:12:59 -0600
Subject: RE: Staining Spurr's Resin-Same Problem

Contents Retrieved from Microscopy Listserver Archives
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OK, for some reason I didn't notice that you were using spurr. Is it at all
possible to use Epon-Araldite instead? It in itself gives better contrast,
for a given staining method.

Garry

Health Sciences Centre
Winnipeg



From daemon Tue Oct 29 14:37:54 2002



From: Fang Mei :      fm46-at-cornell.edu
Date: Tue, 29 Oct 2002 15:18:42 -0500
Subject: Help on TEM replica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, there:

I am using Collodion film to make replicas of a nanobumped silicon
surface (as people do for TEM), and looking at it on AFM. It's easy
to resolve features down to 30nm, but hard at 10nm. Anyone is
experienced to help me out? Any suggestion on different replica
materials other than Collodion? Thanks.

Fang
--
------------------
Department of MSE
Cornell University
Ithaca, NY14853
(607)-2559461
------------------


From daemon Tue Oct 29 14:50:29 2002



From: O'Neil, Ed F ERDC-GSL-MS :      Ed.F.O'Neil-at-erdc.usace.army.mil
Date: Tue, 29 Oct 2002 14:42:42 -0600
Subject: SEM - need help with low-temperature imaging in ESEM

Contents Retrieved from Microscopy Listserver Archives
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I am interested in documenting deformation damage in cement paste during
freezing and thawing cycles. I am using an environmental scanning electron
microscope (Phillips Electroscan 2020) in GSED mode to do my imaging and am
having difficulty obtaining useable images as the temperature drops below 0
C. Even though I am requesting a pressure of 3.5 torr from the microscope
it can only deliver about 0.2 torr and as a result I get almost no useable
image from the microscope. I am new to the ESEM community and would like to
ask if there are any good internet links that can help with low-temperature
imaging techniques using ESEM technology. Any directional help would be
appreciated.

Ed O'Neil
Research Civil Engineer
Concrete and Materials Branch
Geotechnical and Structures Laboratory
US Army Engineer Research and Development Center
Vicksburg, MS 39180


From daemon Tue Oct 29 16:28:07 2002



From: Craig Klotz :      cklotz-at-mcw.edu
Date: Tue, 29 Oct 2002 16:16:07 -0600
Subject: Re: Staining Spurr's resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I use a protocol similar to what Dean mentioned earlier. Sections are
picked up on a grid that has been dipped in 0.01% BSA. The grids are
dried, to adhere the sections, at 70C for about 10 min. If you have
wrinkle/folding problems Dean's toluene-slide warmer method may work
better. For Spurr's resin containing muscle or nerve, I stain the
grids for 20 min. in a methanolic UA solution containing DMSO (90% methanol
sol'n containing 2% UA and 1% DMSO). After a thorough rinsing, the grids
are stained in Reynold's lead citrate for 2 min. Be careful with the DMSO
solution as it will facilitate the absorption of the UA through your skin
(not good!). The longer you let the grids stain in the methanolic UA DMSO
solution, the more intense staining you get to the point where it's too
much. You may have to adjust the times somewhat before you get the desired
effect. One last thing, the methanolic treatment does weaken the sections
some, so when the grid is in scope don't let the sections get "blasted";
keep the beam spread out as much as possible. I hope you will find this
information useful,

Craig M. Klotz B.S., CT (ASCP)
Neuromuscular Pathology
cklotz-at-mcw.edu
EM/Lab Tech.
"Chance favors the prepared mind" - L. Pasteur



From daemon Tue Oct 29 17:31:22 2002



From: Ian Kaplin :      ian-at-mail.emu.usyd.edu.au
Date: Wed, 30 Oct 2002 10:21:28 +1100
Subject: Monte Carlo Program for Mac

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is anyone aware of websites where one can download Monte Carlo
programs for macs? All I am after is one which gives % back-scattered
electrons and an estimation of the reaction volume or depth.
--

Ian Kaplin

Electron Microscope Unit
Madsen Bldg F09
The University of Sydney
NSW 2006
Australia

Tel : 61 2 9351 3302
Fax : 61 2 9351 7682


From daemon Wed Oct 30 03:03:01 2002



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Wed, 30 Oct 2002 09:02:36 +0100
Subject: MSA/EMSA file format help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I`m looking for a more or less official definition of the EMSA/MAS file
format for storing EELS/EDX spectra. I found on the web that this will
be an ISO standard and has name ISO/DIS22029, but on the ISO site I
could not get more info, it seems that the standardisation is not
finished yet.
What I need is either a paper describing the standard or a piece of
software (Fortran,C preferably C++) that reads these files.
I am happy with code that works but is not guaranteed standard, I can
change later to the official standard when it is available. I will use
(and change to C++) this code on a non-commercial basis (GPL probably).

kind regards,

Jo Verbeeck


From daemon Wed Oct 30 08:28:06 2002



From: kbovard-at-creighton.edu
Date: Wed, 30 Oct 2002 08:17:25 -0600 (CST)
Subject: Re: Staining Spurrs--Why use DMSO?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Tue, 29 Oct 2002, Craig Klotz wrote:
} I stain the
} grids for 20 min. in a methanolic UA solution containing DMSO (90% methanol
} sol'n containing 2% UA and 1% DMSO).

Why use the DMSO in the UA solution?

Karen Bovard
EM Lab
Dept. of Pathology
Creighton University Medical Center
Omaha, Nebraska



From daemon Wed Oct 30 08:41:35 2002



From: Jim Quinn :      jquinn-at-www.matscieng.sunysb.edu
Date: Wed, 30 Oct 2002 09:31:15 -0500
Subject: Re: Monte Carlo Program for Mac

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian -

Try the following URL for Monte Carlo Resources.
http://www2.arnes.si/~sgszmera1/mc/mc.html

Several "Mac" solutions are listed.

regards,

Jim



} From Microscopy-request-at-sparc5.microscopy.com Wed Oct 30 05:54:30 2002
} Mime-Version: 1.0
} Date: Wed, 30 Oct 2002 10:21:28 +1100
} To: Microscopy-at-sparc5.microscopy.com
} From: Ian Kaplin {ian-at-mail.emu.usyd.edu.au}
} Subject: Monte Carlo Program for Mac
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is anyone aware of websites where one can download Monte Carlo
} programs for macs? All I am after is one which gives % back-scattered
} electrons and an estimation of the reaction volume or depth.
} --
}
} Ian Kaplin
}
} Electron Microscope Unit
} Madsen Bldg F09
} The University of Sydney
} NSW 2006
} Australia
}
} Tel : 61 2 9351 3302
} Fax : 61 2 9351 7682
}


From daemon Wed Oct 30 09:44:35 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 30 Oct 2002 10:19:49 -0500
Subject: MSA/EMSA file format help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is the description for how to get to the EMSA/MAS libraray -stolen from Nestor.

EMSA/MAS STANDARD FILE FORMAT FOR SPECTRAL DATA EXCHANGE

The following file, EMMFF.DOC, was grabbed directly from the EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National Laboratory. The following FTP Site give you access to these Software Libraries. Access is by anonymous Login:


Host : WWW.AMC.ANL.GOV or
the mirror site WWW.MSA.Microscopy.Com

Login:
Username = Anonymous
Password = Your Email Address


I have a Visual Basic program called EELS_Plot on the EMMPDL that will read, display, overlay five files, and do a few calculations. It will also rewrite the data in the EMSA format with X,Y pairs so that you can open it in a spreadsheet or other program. The program is free.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Jo Verbeeck [mailto:joverbee-at-ruca.ua.ac.be]
Sent: Wednesday, October 30, 2002 3:03 AM
To: MSA listserver


Hi,

I`m looking for a more or less official definition of the EMSA/MAS file
format for storing EELS/EDX spectra. I found on the web that this will
be an ISO standard and has name ISO/DIS22029, but on the ISO site I
could not get more info, it seems that the standardisation is not
finished yet.
What I need is either a paper describing the standard or a piece of
software (Fortran,C preferably C++) that reads these files.
I am happy with code that works but is not guaranteed standard, I can
change later to the official standard when it is available. I will use
(and change to C++) this code on a non-commercial basis (GPL probably).

kind regards,

Jo Verbeeck


From daemon Wed Oct 30 14:34:08 2002



From: Lou Ross :      RossLM-at-missouri.edu
Date: Wed, 30 Oct 2002 14:23:56 -0600
Subject: Room temp. etchant for Alumina

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am sending this post for a student. If you can help, please respond
directly to him. Below you will find a description of his work and
methods, along with his email address for responses.

Thanks,
Lou Ross


I want some information about etching nano-sized fully dense alumina
at room temperature. I have a hot pressed alumina sample with grain
sizes from 50 nm to 250 nm. Currently I am using the following
etchant.
20 ml of nitric acid
20 ml of water
10 ml of HF. Etching time suggested was 15 mins. However it was not enough.
I got this information about the etchant in a book on metallography.
However I am thinking of extending the etching time from 15 mins to
40 mins and 60 mins.
Please let me know whether I could do that. Else, if there are some
alternative etchants at room temperatures for alumina please suggest
me and the time the sample has to be etched.
Please e-mail me at hkq4d-at-missouri.edu.
--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.biotech.missouri.edu/emc


From daemon Wed Oct 30 15:59:29 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 30 Oct 2002 15:47:23 -0600
Subject: NIH Image Questions

Contents Retrieved from Microscopy Listserver Archives
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I am encountering some "challenges" with NIH Image (largely due to a
paucity of computer literacy).

1. Erasure or deletion of parts of an image now leave a black, rather
than white, area. How can I reverse this situation so that deletions
yield a white area in a normal image?

2. How would one write a script so that the Watershed operation can
be given from the keyboard rather than the pull down menu? This
command is located in Process } Binary } Watershed.

Thank you and sorry if these are simple questions.

John

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
##############################################################


From daemon Wed Oct 30 17:09:23 2002



From: RCHIOVETTI-at-aol.com
Date: Wed, 30 Oct 2002 17:59:47 EST
Subject: Low Mag Photoeyepiece?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

I am looking for a low magnification projection photoeyepiece. These were
once made by Olympus and probably by others as well.

I need a standard 23mm diameter 1.6x or 2.0x photoeyepiece. If you have one
of these and if it needs a new home, please contact me off-list.

Thanks very much.

Bob Chiovetti
(rchiovetti-at-aol.com)


From daemon Wed Oct 30 18:26:47 2002



From: James Martin :      james.s.martin-at-att.net (by way of
Date: Wed, 30 Oct 2002 18:17:41 -0600
Subject: MTI-PUREGAS purge gas generation systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would anyone comment -- OFF-LIST only -- about their experience with
MTI-PUREGAS portable purge gas generation systems? Thank you.

James Martin


From daemon Wed Oct 30 22:24:23 2002



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Thu, 31 Oct 2002 14:19:28 +1000
Subject: Balzars Parts

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

We have recently obtained an old Balzers BAF301 freeze fracture unit
which we are trying to recondition. Unfortunately some parts are
missing. We are in need of a stage. We would like to obtain a counter
flow loading system(e.g. specimen table and flange for quick release).
Also we are keen to obtain a rotary cold table. So we're wondering if
there is anybody who has any type of a Balzers freeze fracture unit that
is sitting around doing nothing and who is willing to relinquish these
parts to us for a reasonable price.


Regards
JVN
--
John V Nailon
Executive Officer and Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld



From daemon Thu Oct 31 07:09:55 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 31 Oct 2002 12:56:21 -0000
Subject: Critical point drying - chloroform as intermediate solvent?

Contents Retrieved from Microscopy Listserver Archives
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I have a job which requires a vigorous lipid/wax extraction step
followed by critcal point drying.
The lipid/wax solvent will be chloroform. Can I go straight into the
(CO2) critical point drier from chloroform,
or am I going to blow myself up?

Best wishes
Chris

Dr. Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Tel +44 131 650 5554
FAX +44 131 650 8668
Mobile 07710 585 401



From daemon Thu Oct 31 08:06:04 2002



From: sstouden-at-thelinks.com
Date: Thu, 31 Oct 2002 07:57:52 -0600 (CST)
Subject: Re: Fixation of mitochondria (Muscle TEM)

Contents Retrieved from Microscopy Listserver Archives
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Rs White , i notice cell cycle stage, and cell physiology. But what I do
not notice is in which CC stage and which physiology states determine the
morphological variants.

let me start first with the question: thoughout all known variations of
cell cycle stages and physiolgical variation: what would be the range of
changes? that is what changes have been noted and which of the variables
is responsible for that change.

1. DNA changes?
2. membrane changes?
3. metabolism changes?
4. membrane changes (both inter mitochrondrial and outer m. cytoplasmic
chges)
5. organelle changes?
6. one for example, is receptor mediated glut 4 metabolism in
mitochrondria: which interest me considerable.
thanks.
sterling


On Mon, 28 Oct 2002, Rosemary White wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Re. morphology of mitochondria, as noted by others below, it's quite
} variable at TEM level, and fixation will affect it substantially. Number,
} shape, size and ultrastructure of mitochondria in a particular cell type
} will vary depending on stage in cell cycle, cell physiology - whether
} quiescent or not, activated by stimulus or not, etc, etc. Also varies with
} cell type. For good ultrastructural preservation, I would use rapid
} freezing and freeze substitution in preference to chemical fixation.
} What do you mean by "pores" in mitochondria?
} cheers,
} Roseamry
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } -----------------------------------------------------------------------.
} }
} }
} }
} } pardon me, but i would like to ask very simple question, which I am sure
} } everyone else on this list but me is able to answer,
} }
} } what is the morphology of a standard mitochrondium:
} }
} } how many standard mitochrondia structures are there?
} } do structures appear and disappear within the same mitochrondrium
} } Does cell type of origin change the standard of the structure?
} } Is there a mitochrondria database somewhere?
} } what about pore type intra mitochondrial and intercompartmental?
} } pore distribution function and density seems to be the big determinate of
} } mitochrondiral differentiation and function.
} } what are they.
} } the big structure we all know, it the little ones, that I am talking
} } about, some that are sometimes there and sometimes not.
} }
} }
} }
} } how are the different structures separately classified in each such
} } mitochrondria?
} } is there a measurement technique to determine exact location and volume of
} } these intra mitochrondrial structures?
} }
} } is there a noticeable by experimental method difference between
} } mitochrondria of one source or the other other.
} }
} } thanks for indulging my ignorance.
} } sterling
} }
} } On Tue, 22 Oct 2002, Sergey Ryazantsev wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Mitochondria is very sensitive to so many factors, which we practically
} } } could not count. Oxidative stress is one of the major problem here. "Well
} } } fixed" mitochondria is only "reflection" of the real structure, not real
} } } things itself (as any other chemically fixed structure). I could imagine
} } } the experiment, when we will take/process samples at the very identical
} } } conditions. In this case we will probably see very small mitochondria
} } } structure variation from sample to sample. In practice, there are so many
} } } factors we could not count/reproduce. Therefore we expect to have a
} } } diversity of the structure presentation from the identical organelle,
} } } mitochondria in our case. So, we have a "distribution" of the
} } } structure. If we are studying some "effect on mitochondria" then we have
} } } to compare two "distributions" from control and experiment. If the
} } } "distributions" are not cross overed, we could talk about some
} } } changes. This is simplification of the problem of coarse. In my point of
} } } view, it's relatively simply to compare such "distributions" on the level
} } } of morphological changes and much, much more difficult on quantitative
} } } level (if even possible). In general, the data analysis is not only
} } } scientific issue, it's also ethical issue. I did see people who was
} } } extremely smart manipulating the EM data in favor of their theories...
} } }
} } } As for direct question in original posting, just general recommendations:
} } } take smallest possible piece of tissue, immediately immerse them into the
} } } fixer and cut on smaller pieces in the fixer to 1x1x1 mm with new scalpel,
} } } then cut each piece to 0.5x0.5x0.5 mm, then move tissue in the fresh fixer
} } } and fix 1-2 hours on ice. I usually do everything on ice, but you may try
} } } to do first step (cutting tissue, 1st change of the fixer) at the room temp
} } } to reduce temperature shock (use FRESH fixer for the change!). Personally,
} } } I prefer 1x PBS instead cacodylate and 1.5% GA is enough for such small
} } } pieces. Then I would wash tissue with 1x PBS for 30 min and fix in 1% OSO4
} } } (in PBS or just in water) for 1 h ON ICE. 30 min water wash
} } } thereafter. All solutions should be fresh and made from "EM grade"
} } } chemicals and 18 MOhm "cell culture quality" deionized water. I am using
} } } GA solutions no longer than one hour after preparation if stored on
} } } ice. OSO4 needs to be prepared just before use and stored on ice. Time
} } } between collecting the tissue/animal death and fixation should be 20-30
} } } seconds or SHORTER if we are talking about "good" mitochondria fixation.
} } } The way you kill animal have dramatic effect on mitochondria!
} } } Good luck. Sergey.
} } }
} } }
} } } At 08:30 AM 10/23/02 +1300, you wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Kia Ora
} } } }
} } } } Marcs question about mitochondria is a little timely for us.
} } } }
} } } } In our Unit recently we have been experiencing an increase in the number
} } } } of investigators who are wanting to quantify mitochondria morphology,
} } } } usually after some experimental change.
} } } }
} } } } eg 1, changes in muscle mitochondria in resistance trained athelete's
} } } } (weight lifters) compared to endurance trained athelete's (runners) (Human)
} } } }
} } } } eg 2, changes in brain stem mitochondria after a particular anaesthetic
} } } } and neuro-surgical procedure is used (Rat)
} } } }
} } } } eg 3, mitochondria changes in various tissues of hibernating hedgehog
} } } } compared to non hibernating hedgehog
} } } }
} } } } eg 4, mitochondria changes in fish as they move from sea water 'lifestyle'
} } } } to a fresh water 'lifestyle'.
} } } }
} } } } eg 5. mitchondria changes in cultured cells after biochemically induced.
} } } } apoptosis.
} } } }
} } } } These projects are all looking at morphological changes of the
} } } } mitochondria rather than chnages in the number of mitochondria.
} } } }
} } } } I have some niggly concerns about these projects but I can't really put a
} } } } finger on it. Mitochondria are such sensitive souls that I am not
} } } } entirely convinced that some of the changes being noted are not tissue
} } } } dissection related or fixation related (ie some are perfused, some are
} } } } immersion fixed etc) rather than actually experiment related.
} } } }
} } } } My niggles are not because of something really obvious like an obvious
} } } } osmotic problem, or delays in fixative penetration, or using poor quality
} } } } aldehyde. It is just a gut feeling. Perhaps it is simply different
} } } } 'appearances' of mitochondria in the diferent tissues.
} } } }
} } } } So I don't have a specific question, more I would interested in peoples
} } } } comments on assessing the quality of mitochondria fixation from a
} } } } morphological point of view rather than a technique point of view.
} } } }
} } } } I haven't been able to find any good reference material, apart from that
} } } } which covers technique problems, that has been able to dispel my niggle.
} } } }
} } } } Anyone got any thoughts on precautions to take in preparation when
} } } } specifically focusing on mitochondria morphology ?
} } } }
} } } } Thanks
} } } }
} } } } Allan
} } } }
} } } }
} } } } } ------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } -----------------------------------------------------------------------.
} } } } }
} } } } }
} } } } } Dear List,
} } } } }
} } } } } I would appreciate recieveing any fixation protocol you know
} } } } } works well for mouse muscle (extensor digitorum longus (EDL)
} } } } } and epitrochlearus (EPI)). We use routinely immersion into
} } } } } 2.5% glutaraldehyde in 0.1M cacodylate pH 7.4, but have had
} } } } } often problems with mitochondrial swelling. Since this project is
} } } } } to quantify mitochondrial density, we do need perfect and
} } } } } reliable fixation of all mitos. Perfusion is not an option. Thanks
} } } } } for any advise on type of fixative, buffer and temperature.
} } } } }
} } } } } Marc
} } } } } --
} } } } } Marc Pypaert
} } } } } Department of Cell Biology,
} } } } } Center for Cell and Molecular Imaging and
} } } } } Ludwig Institute for Cancer Research,
} } } } } Yale University School of Medicine
} } } } } 333 Cedar Street
} } } } } New Haven CT 06520
} } } } } Tel : (203) 785 3681
} } } } } Fax : (203) 785 7446
} } } }
} } } } --
} } } } -------------------------------------------------
} } } } Allan Mitchell
} } } } Technical Manager
} } } } Otago Centre for Electron Microscopy
} } } } C/-Department of Anatomy and Structural Biology
} } } } School of Medical Sciences
} } } } P.O. Box 913
} } } } Dunedin
} } } } New Zealand
} } } }
} } } } Phone (03) 479 5642 or 479 7301
} } } } Fax (03) 479 7254
} } } }
} } } } Unit: http://www.otago.ac.nz/anatomy/emunit/
} } } } Department: http://anatomy.otago.ac.nz/
} } } }
} } } }
} } } }
} } } } "Life is a gift, don't waste it"
} } }
} } }
}
}
} Dr Rosemary White rosemary.white-at-csiro.au
} Microscopy Centre fax 61- 2 6246 5000
} CSIRO Plant Industry ph. 61- 2 6246 5475 or
} GPO Box 1600 mob. 61- 0402 835 973
} Canberra, ACT 2601, Australia
}
}
}



From daemon Thu Oct 31 08:06:08 2002



From: Jon Ekman :      ekman-at-bio.fsu.edu
Date: Thu, 31 Oct 2002 09:04:47 -0500
Subject: epoxy resin for 300keV TEM

Contents Retrieved from Microscopy Listserver Archives
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I am forwarding a message from a user concerned that the following epoxy
resin may not be "good" for our 300keV FEG TEM. You may send replies to
the list or to Kim Riddle: riddle-at-bio.fsu.edu

TIA, Jon

Hello all,

Has anyone used Aeropoxy's PR2032/PH3660 resin as an embedding media for
TEM? It's an epoxy laminating resin used in fabricating composite parts for
the aeronautical industry. Specially formulated for engine cowlings, wing
spars, landing gear legs, etc.

Thanks, Kim



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Kimberly A. Riddle
Florida State University tel: 850.644.6519
Biological Science Imaging Resource
119 Bio Unit I, 4370 fax: 850.644.0481
Tallahassee, FL 32306 riddle-at-bio.fsu.edu
http://bsir.bio.fsu.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From daemon Thu Oct 31 10:30:04 2002



From: Ann-Fook Yang :      yanga-at-agr.gc.ca
Date: Thu, 31 Oct 2002 11:18:03 -0500
Subject: Re: SEM - need help with low-temperature imaging in ESEM

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Hi Ed,

Make sure the water bottle is not empty. If there is water, the valve may be faulty.
Are you able to get more than 5 Torr at 2C when using 500 mm PLA? My XL30 ESEM works that way and it goes above 3.5 Torr when the temp drops to -2.5C. As expected, a lot of water condensed on the stage.
I don't know any web links. You may ask Daniel Phifer at FEI DPhifer-at-FEICO.com



AnnFook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Room 2091, Bldg. 20,
Central Experimental Farm,
Ottawa, Ontario
Canada K1A 0C6

Tel: 1-613-759-1638
Fax: 1-613-759-1701

e-mail: yanga-at-em.agr.ca

} } } "O'Neil, Ed F ERDC-GSL-MS" {Ed.F.O'Neil-at-erdc.usace.army.mil} 10/29/02 03:42PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am interested in documenting deformation damage in cement paste during
freezing and thawing cycles. I am using an environmental scanning electron
microscope (Phillips Electroscan 2020) in GSED mode to do my imaging and am
having difficulty obtaining useable images as the temperature drops below 0
C. Even though I am requesting a pressure of 3.5 torr from the microscope
it can only deliver about 0.2 torr and as a result I get almost no useable
image from the microscope. I am new to the ESEM community and would like to
ask if there are any good internet links that can help with low-temperature
imaging techniques using ESEM technology. Any directional help would be
appreciated.

Ed O'Neil
Research Civil Engineer
Concrete and Materials Branch
Geotechnical and Structures Laboratory
US Army Engineer Research and Development Center
Vicksburg, MS 39180




From daemon Thu Oct 31 11:12:19 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 31 Oct 2002 11:30:13 -0600
Subject: Re: Critical point drying - chloroform as intermediate solvent?

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Dear Fang,
I was taught that the resolution of the replica is related to the molecular
weight, i.e. the size of the molecule of the replication material. You may
be getting close to the resolution of the collodion at 10 nm. I have used
carbon replicas for TEM work, but I don't know if they are robust enough for
AFM. They are easy enough to try: evaporate carbon onto the surface, then
place the material in an etchant to release to carbon. It should float to
the surface of the etchant, where you can scoop it up on a substrate
suitable for AFM.
Other than that, look for a replication materials with a smaller molecule.
Good luck.
Mary

----- Original Message -----
} From: "Fang Mei" {fm46-at-cornell.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, October 29, 2002 12:18 PM


Chris,

No. I don't think you'll blow up yourself, but your specimens won't
be happy. We've been doing a chloroform extraction of lipids for
cheese SEM, and after the extraction, the specimens have to go back
to 100% absEtOH (through a 1:3, 1:1, 3:1 series) before going into
the SEM.

Phil

} I have a job which requires a vigorous lipid/wax extraction step
} followed by critcal point drying.
} The lipid/wax solvent will be chloroform. Can I go straight into the
} (CO2) critical point drier from chloroform,
} or am I going to blow myself up?
}
} Best wishes
} Chris
}
} Dr. Chris Jeffree
} University of Edinburgh
} Biological Sciences EM Facility
} Tel +44 131 650 5554
} FAX +44 131 650 8668
} Mobile 07710 585 401

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Thu Oct 31 14:07:53 2002



From: Marcello Andreeta :      marcello-at-if.sc.usp.br
Date: Thu, 31 Oct 2002 16:55:58 -0200
Subject: transmitted interference microscope

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir/Madam

I am interested in buying a transmitted interference microscope. Do you
know any company that sells them?

Thank you in advance,

Dr. Marcello R. B. Andreeta
marcello-at-if.sc.usp.br



From daemon Thu Oct 31 15:01:32 2002



From: Craig Klotz :      cklotz-at-mcw.edu
Date: Thu, 31 Oct 2002 14:45:48 -0600
Subject: RE: DMSO

Contents Retrieved from Microscopy Listserver Archives
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The use of DMSO is suggested in Dr. Bozzola's book as a possible way to
enhance the penetration of the UA into the plastic. Personally, I have
noticed an increase in contrast when using UA w/ DMSO as opposed to w/o
DMSO. For reference, my sections are 75-80nm thick and contain muscle
and/or nerve exclusively.

Craig M. Klotz B.S., CT (ASCP)
Neuromuscular Pathology
cklotz-at-mcw.edu
EM/Lab Tech.
"Chance favors the prepared mind" - L. Pasteur



From daemon Thu Oct 31 17:08:21 2002



From: Dr.ROTIMI ADEMOLA :      aderotimi2-at-netscape.com
Date: Fri, 1 Nov 2002 00:07:21 +0000
Subject: response required

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. ROTIMI ADEMOLA
Tel: 234 28 106782
Fax: 234 17 597626

Request for assistance: Strictly Confidential

I am Dr. ROTIMI ADEMOLA the chairman of contract award and review committee set up by the federal government of Nigeria under the present civilian dispensation to award new contracts and review existing ones. I came to know of you in my search for a reliable and reputable person to handle a very confidential transaction, which involves the transfer of a huge sum of money to a foreign account.

There were series of contracts executed by a consortium of multinationals in the oil industry in favour of N.N.P.C. The original value of these contracts were delibrately over invoiced to the sum of USD$25,000,000.00 (twenty five million united state dollars). This amount has now been approved and is now ready to be transferred being that the companies that actually executed these contracts have been fully paid and the projects officially commissioned.

Consequently, my colleagues and I are willing to transfer the total amount to your account for subsequent disbursement, since we as civil servants are prohibited by the code of conduct bureau (civil service law) from operating and/or opening foreign accounts in our names. Needless to say, the trust reposed on you at this juncture is enormous, in return, we have agreed to offer you 20% of the transferred sum, while 10% shall be set aside for incidental expenses (internal and external) between both parties in the course of the transaction you will be mandated to remit the balance to other accounts in due course.

Modalities have been worked out at the highest level of the ministry of finance and the Central bank of Nigeria for the immediate transfer of the funds within 14 working days subject to your satisfaction of the above stated terms. Our assurance is that your role is risk free. To accord this transaction the legality it deserves and for mutual security of the funds the whole approval procedures will officially and legally processed with your name or the name of any company you may nominate as the bonafide beneficiary. Once more I want you to understand that having put in over twenty five years in the civil service of my country, I am averse to having my image and career dented.

This matter should therefore be treated with utmost secrecy and urgency it deserves. Please you should signify your intention to assist by giving me a call on my direct line (234 28 106782) or send me a fax on my confidential fax number ( 234 17 597626 ) so that I can brief you further. I want to assure you that this business proposal is 100% risk free as we have done our home work properly. I quite believe that you will protect our interest by taking this deal strictly confidential, as we are still in government service, which we intend to retire from. kindly expedite action as we are behind schedule to enable us include this transfer in the first batch which would constitute the last quarter payments for the 2002 financial year.


Thanks and God bless.

Dr. ROTIMI ADEMOLA.






From daemon Fri Nov 1 10:56:16 2002



From: Vaughn Fierke :      Vfierke-at-SNBLUSA.com
Date: Fri, 1 Nov 2002 08:44:26 -0800
Subject: TEM SEM water chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning,
I'm looking for a water chiller for a TEM and SEM.
The unit looking for is a Haskris R075 or equivalent.
Options:
water cooled
1 supply, 2 returns(minimum)
Hot gas bypass preferable, but not necessary
115VAC

Thanks in advance for any info, advice, recommendations.
Vaughn Fierke
SNBL USA, Ltd

Confidentiality Notice: This email, its contents and attachments are
confidential and may contain privileged information. It is intended solely
for the use of addressee(s) only. Any use, copying or disclosure of this
communication or attachments to any other person is expressly prohibited
without written permission of SNBL-USA,Ltd. If you receive this message in
error, please notify the sender at SNBL USA, Ltd. immediately by return
e-mail, telephone +1 425 407 0121, or fax +1 425 407 8601. We appreciate
your cooperation




From daemon Fri Nov 1 12:54:36 2002



From: Lauren :      simmerman_2000-at-netzero.com
Date: Fri, 1 Nov 2002 18:45:19 GMT
Subject: TEM-Need help with problem Nerves

Contents Retrieved from Microscopy Listserver Archives
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Hi,
We are part of a clinical pathology lab, so we do a lot of muscle,
nerve & kidney biopsies. The nerves have been a continual problem.
They rarely want to lay flat, once dried on the hot plate. This causes
a lot of frustration for us and the doctors.
The nerve cross-sections are cut at 1000nm, and then dried on the hot plate (95-100 degrees C). After staining with Toluidine Blue, the creases and bubbles are very clear. We have not experienced this problem with any of the other tissues.

Does anyone have any suggestions to get the sections to lay flat on the glass slide?

Thank You,
Lauren Simmerman
NHS EM Lab
Omaha, NE
402-559-7729





From daemon Fri Nov 1 15:45:24 2002



From: Angela Klaus :      avklaus-at-amnh.org
Date: Fri, 1 Nov 2002 16:32:57 -0500 (EST)
Subject: Museum Session at Scanning 2003

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

Just a quick note to mention that the session "Museum Applications of SEM"
will take place again at Scanning 2003 (San Diego, May 3-5th;
http://www.scanning-fams.org).

The deadline for abstract submission is Dec. 2nd. The museum session
debuted at Scanning 2001 and was very successful. Looking forward to an
interesting program again this year.

Best regards,

Angela

-----------------------------------------
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------



From daemon Fri Nov 1 17:11:14 2002



From: Anne Peattie :      apeattie-at-uclink.berkeley.edu
Date: Fri, 1 Nov 2002 15:03:49 -0800
Subject: ESEMs in Bay Area?

Contents Retrieved from Microscopy Listserver Archives
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I am looking for an ESEM that will resolve 100nm-wide structures
under minimal evacuation, which can also be modified (reversibly, of
course) for manipulation experiments. Does anyone know of a facility
in the San Francisco Bay Area with these capabilities? I am a
graduate student at UC Berkeley with considerable SEM experience. I'd
be happy to provide a more detailed explanation of my research if
necessary.

Thank you,
Anne Peattie
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
PolyPEDAL Lab
University of California, Berkeley
Department of Integrative Biology
3060 VLSB, #3140
Berkeley, CA 94720-3140
(510) 643-5183
http://polypedal.berkeley.edu/anne


From daemon Fri Nov 1 17:13:38 2002



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Fri, 1 Nov 2002 17:59:41 -0500
Subject: TEM-Need help with problem Nerves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nerves can be a real challenge. Here's my mantra for nerves: Leave in half
and half overnight (Spurr). Use only great glass knives. Be picky and do
not use any knife with only an OK edge. We cut at 0.3 to 0.5 microns (if
my math is correct you are cutting at 1 micron); I pick up specimens with
the beveled tip of a TB or equiv needle. Make sure that the tip is smooth
and not jagged and scrupulously clean (clean with 100% EtOH and wipe with
lens tissue). Slides are dried on a hot plate at a lower temp than you:
probably around 65-70 degrees for 15 min.
Block face 0.5 to 1.5 mm.
When staining: take slide with heated Tol Blue off of hot plate and immerse
in HOT tap water, not COLD. Rinse in running hot tap water. Dry completely
on hot plate. (Walk away and do something else). Let slide come to room
Temp, then dip in xylene, coverslip with standard histo mounting media
(Cytoseal, etc,).

Good luck and let me know if any of this helps.
Becky Garrison
Pathology Supervisor
904-244-6237; 6067

-----Original Message-----
} From: Lauren [mailto:simmerman_2000-at-netzero.com]
Sent: Friday, November 01, 2002 1:45 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,
We are part of a clinical pathology lab, so we do a lot of muscle,
nerve & kidney biopsies. The nerves have been a continual problem.
They rarely want to lay flat, once dried on the hot plate. This causes
a lot of frustration for us and the doctors.
The nerve cross-sections are cut at 1000nm, and then dried on the hot plate
(95-100 degrees C). After staining with Toluidine Blue, the creases and
bubbles are very clear. We have not experienced this problem with any of the
other tissues.

Does anyone have any suggestions to get the sections to lay flat on the
glass slide?

Thank You,
Lauren Simmerman
NHS EM Lab
Omaha, NE
402-559-7729





From daemon Fri Nov 1 18:32:36 2002



From: Jeffrey Smith :      smith.jeffrey.w-at-worldnet.att.net
Date: Fri, 1 Nov 2002 18:23:29 -0600
Subject: Reichert

Contents Retrieved from Microscopy Listserver Archives
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I have a Reichert binocular microscope, ivory in color, that is probably
from the 1960's or so. How can I look up the serial numbers to gain
information about this scope. I also have about 5 wooden boxes of
assorted objectives, oculars, condensers, and a camera. Thanks for any
help you can provide.



From daemon Sun Nov 3 10:48:08 2002



From: Cochran :      fisher-at-meganet.net (by way of MicroscopyListserver)
Date: Sun, 3 Nov 2002 10:28:37 -0600
Subject: Digital camera

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

I have been asked to investigate the upgrade of several Nikon
microscopes to digital cameras. I welcome any recomendations based
upon experience or the sharing of any difficulties experienced in
your labs since the transition.

Thanx,
Ray


From daemon Sun Nov 3 20:53:08 2002



From: =?GB2312?B?0dXPyMn6?= :      richard-at-51translation.com
Date: Mon, 4 Nov 2002 10:39:26 +0800
Subject: =?GB2312?B?zsS1tbet0uuhor/a0uu3/s7xoaLN+NW+sb612Luv?=

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Subject Quotation

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From daemon Sun Nov 3 23:18:43 2002



From: ÍøÂçÏÈ·æ :      davedudd-at-ah163.com
Date: Mon, 4 Nov 2002 13:09:13
Subject: ×î½ü»¹ºÃÂð£¡

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From daemon Mon Nov 4 09:25:30 2002



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Mon, 4 Nov 2002 17:07:19 +0200
Subject: RE: ???????????????

Contents Retrieved from Microscopy Listserver Archives
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say again?


Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gaborone
Botswana

Phone : +267 355 2426/5222
Mobile : +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw



} -----Original Message-----
} From: richard-at-51translation.com [mailto:richard-at-51translation.com]
} Sent: Monday, November 04, 2002 4:39 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: ???????????????
}
}
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From daemon Mon Nov 4 09:43:55 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Mon, 04 Nov 2002 16:35:37 +0100
Subject: TEM Sample prep: 10-100 micron scale samples

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
Has anyone ever prepared TEM specimens from small samples of the size
10-100 microns. We have a student who is studying phase transitions in
a diamond anvil cell and wants to put the samples in the TEM. The
resulting samples are usually of the order of 50 microns in diameter,
and rather thinner in cross section.
This falls in an inconvenient size range: I normally work with either
bulk materials which can be thinned, or with nanoscale particles which
can be dispersed in something and dropped onto a holey carbon film.
I was wondering if there was perhaps some way of embedding the samples
and then thinning the resulting composite.
The material is a hard glassy or crystalline ceramic, depending on the
exact treatment in the diamond anvil cell.

Thanks for any leads you can give.

Best wishes
--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Mon Nov 4 13:33:49 2002



From: Shu-You Li :      syli-at-northwestern.edu
Date: Mon, 04 Nov 2002 13:12:32 -0600
Subject: Re[2]: ???????????????

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor,

Please ban this address richard-at-51translation.com or even everything
from 51translation.com. It is a SPAM written in Chinese. I know most of the listers can not
read it, although I can :-).

Shuyou.

On Mon, 4 Nov 2002 17:07:19 +0200
"Coetzee, Mr S. H Physics Science" {COETZEES-at-mopipi.ub.bw} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} say again?
}
}
} Mr S. H. Coetzee
} Electron Microscope Unit
} University of Botswana
} Private Bag 0022
} Gaborone
} Botswana
}
} Phone : +267 355 2426/5222
} Mobile : +267 718 96 729
} Fax : +267 585 097
} e-mail : coetzees-at-mopipi.ub.bw
}
}
}
} } -----Original Message-----
} } From: richard-at-51translation.com [mailto:richard-at-51translation.com]
} } Sent: Monday, November 04, 2002 4:39 AM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: ???????????????
} }
} }
} } --------------------------------------------------------------
} } ----------
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} } of America
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} ~{5DR; {RJ5A&P[:q5DW(R57-~}
} } ~{Rk7~NqLa9)IL!#1} 9+K} SI2)J? {0WJ~}
} } ~{In7-RkW( {R9\-at-m#,QO8qV4PP~}ISO9002~{VJA?1#UOLeO5#,7~Nq76N'If {04,20!"5gWS!";z~}
} ~{P5!"WT6/;/!"VF-at-d!";/9$!"~}
} } ~{M(Q6!"MxBg!"M?W0!"=(V~!"RG1m5H~}
} } ~{8wPPR5AlSr~},~{?M;'0Y7VV.0Y5DBzRbJGNRCG2;P85DW7Gs~}.~{SHFdJGTZIO:#RkP-5D4sA&V'3VSk~}
} ~{P-VzOB#,RQSk~}IBM~{!"~}
} } TOSHIBA~{!"~}Siemens~{!"~}Roche~{!"~}NOVARTIS~{!"~}
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} }
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} ~{O5!#?gHk~}21~{J-at- {M#, {NPE=+H+~}
} } ~{Cf9a39~}ISO9002~{5D7~Nq1jW {LeO5#,TK~}
} } ~{SCOH=x5D9\-at-mD#J= {0PBS15D} -S*-at-mDn#,=_3ON*:#DZMb8wFsJBR55%N; {0EsSQLa9)WnN*7E~}
} ~{PD!"V\5=5D7-Rk7~Nq!#~}
} }
} } ~{NRCGOVTZU}:MNwCEWS9$R5WT6/;/9+K} RT {00"6{?(LX!"146{0"6{?(LX1#3VWEA {:C5D~}
} ~{:OWw9XO5!#NRCGKfJ19':r~}
} } ~{Dz5D9bAY:MWIQ/~},~{NRCG=+FZ4}WESkDc~}
} } ~{5D3$FZ:OWw~},~{9+K} H+LeT19$=+RTWn4s5DHHGi:MWn3OV?5D7~NqL,6HN*DzP'-at-M!#~}
} }
} }
} }
} } ~{K3W#~}!
} } ~{ILlw~}!
} }
} }
} }
} } ~{IO~}
} } ~{:#~} ~{ {N~} ~{PE~} ~{7-~} ~{Rk~} ~{SP~} ~{O^~} ~{9+~} ~{K} ~}
} }
} } ~{JP3!~}
} } ~{2?~} ~{QUNuAA~}
} }
} } Tel
} } ~{#:~}021-65448331*808
} }

_____________________________
Shu-You Li, Ph.D.
Electron Microscopist
Electron Probe Instrumentation Center(EPIC)
Northwestern University
2220 Campus Drive, 1141 Cook Hall
Evanston, IL 60208, USA
Ph: (847) 491-7798, Fax: (847) 491-7820
Email: syli-at-northwestern.edu; syli16-at-hotmail.com
http://pubweb.northwestern.edu/~~sli974





From daemon Mon Nov 4 14:14:38 2002



From: Hong Yi :      hyi-at-emory.edu
Date: Mon, 4 Nov 2002 15:05:30 -0500
Subject: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a good EM atlas to buy. Any recommendations? Thank
you.

Hong



From daemon Mon Nov 4 18:55:21 2002



From: Dwight Arrieche (IIBCA) :      darriech-at-cumana.sucre.udo.edu.ve
Date: Mon, 4 Nov 2002 20:43:39 -0400 (VET)
Subject: San Antonio 2003

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopist

I am looking for some information on the next meeting listed below, but
could not find a link at
http://www.msa.microscopy.com/MSAMeetings/MSAMeetings.html

Microscopy & Microanalysis 2003

Sponsored by MSA, MAS, and CIASEM

August 3-7 in San Antonio Texas.

Greatly appreciate your help



From daemon Tue Nov 5 01:48:18 2002



From: =?ISO-8859-2?Q?Old=F8ich_Benada?= :      benada-at-biomed.cas.cz
Date: Tue, 05 Nov 2002 08:37:43 +0100
Subject: Re: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
A really good book (not only the atlas) with lot of
images and valuable comments is:
BIOMEDICAL ELECTRON MICROSCOPY by A.V. Maunsbach and
B.A. Afzelius, Academic Press 1999.
Best regards Oldrich Benada

On 4 Nov 2002 at 15:05, Hong Yi wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am looking for a good EM atlas to buy. Any recommendations? Thank
} you.
}
} Hong
}
}
}


+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-241062399
Fax: +420-241062347
WEB: http://www.biomed.cas.cz/mbu/lem113/lem.htm




From daemon Tue Nov 5 08:28:45 2002



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Tue, 5 Nov 2002 09:16:34 -0500 (EST)
Subject: Re: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Try Cross and Mercer, Cell and TIssue Ultrastructure, A functional
perspective, Freeman Press.

DL

On Mon, 4 Nov 2002, Hong Yi wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am looking for a good EM atlas to buy. Any recommendations? Thank
} you.
}
} Hong
}
}
}
}

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718






From daemon Tue Nov 5 08:49:11 2002



From: LEVIN, Martin A. (Biology) :      LEVIN-at-easternct.edu
Date: Tue, 5 Nov 2002 09:41:14 -0500
Subject: RE: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use a textbook in my ultrastructure class called, "Cell and Tissue
Ultrastructure: A Functional Perspective," by Patricia Cross and K. Lynne
Mercer. Although it is getting a bit old (1993), it still has a good
assortment of electron micrographs. The small amount of text is at times
dated.

Martin A. Levin
Director of the Center for Educational Excellence (CEE)
and Professor of Biology
Eastern Connecticut State University
J. Eugene Smith Library, Room 431
Willimantic, Connecticut 06226
(860)465-5589/4324
FAX (860)465-5522/5213
Email: levin-at-easternct.edu



} ----------
} From: Hong Yi
} Sent: Monday, November 4, 2002 3:05 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: EM Atlas
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am looking for a good EM atlas to buy. Any recommendations? Thank
} you.
}
} Hong
}
}
}


From daemon Tue Nov 5 08:53:17 2002



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Tue, 05 Nov 2002 09:43:46 -0500
Subject: SEM/EDS - bacteria on limestone, etc

Contents Retrieved from Microscopy Listserver Archives
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Hi group - not owning a critical point dryer, etc., what would be the
best solution in preparing a fresh core sample from fresh water so that
the bacteria will not become desiccated? I do have opportunity to run
SEM on low vacuum which will help.
Thanks
Barb



From daemon Tue Nov 5 09:50:45 2002



From: Thomas Weber :      thomas.weber-at-mssm.edu
Date: Tue, 05 Nov 2002 10:43:31 -0500
Subject: TEM: Immuno Staining / Negative Staining of virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I'm trying to immuno gold stain adeno-associated virus adsorbed to
parlodion coated copper grids. I UV-irradiate the grids for 30 min. to
help the virus stick. If I just uranyl acetate stain the sample, I see
plenty of viral particles. However, after the immuno staining
procedure (1: Wash, 2: primary AB, 3: Wash, 4: secondary AB, 5: wash,
6: uranyl acetate staining) I can't detect any virus particles anymore.
All I see is low background staining with immuno gold.

I would appreciate any suggestions. Is there any way to crosslink the
virus to the grid? Or would fixing with Glutaraldehyde before or after
adsorption help?

I'm not a regular visitor to this list, I would appreciate if you could
e-mail me a copy of the answer directly to: thomas.weber-at-mssm.edu.


Thanks for any help.


Thomas

P.S.: I don't have really access to a glow discharge unit.



From daemon Tue Nov 5 10:42:08 2002



From: Jeff Stewart :      jeff-at-metallography.com
Date: Tue, 05 Nov 2002 11:32:45 -0500
Subject: Re: San Antonio 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dwight,

I have some information posted at my website regarding the Call for Papers
etc. for the San Antonio meeting which might be helpful although it's from
an International Metallographic Society (one of the participating
organizations) perspective. Take a look at
http://www.metallography.com/ims/2003call.htm and
http://www.metallography.com/ims/2003.htm.

Jeff Stewart
Metallographic Laboratory Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703


"Dwight Arrieche (IIBCA)" wrote:

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} -----------------------------------------------------------------------.
}
} Dear Microscopist
}
} I am looking for some information on the next meeting listed below, but
} could not find a link at
} http://www.msa.microscopy.com/MSAMeetings/MSAMeetings.html
}
} Microscopy & Microanalysis 2003
}
} Sponsored by MSA, MAS, and CIASEM
}
} August 3-7 in San Antonio Texas.
}
} Greatly appreciate your help



From daemon Tue Nov 5 11:17:06 2002



From: Dorothy Sorenson :      dsoren-at-umich.edu
Date: Tue, 05 Nov 2002 12:06:26 -0500
Subject: pre-embedding immunogold TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I am trying to do pre-embedding ultrasmall-gold immunolabeling of mouse
optic nerve. I am trying to do it on 10 um-thick frozen sections attached
to gelatin-coated superfrost/plus glass microscope slides. I am in the
middle of the first run on these samples, and I am finding that my first
challenge is to get the tissue to stick to the slides. For fluorescence
immuno-labeling, one can allow the frozen sections to dry onto the slides.
For TEM, though, the tissue can?t dry out. I froze the slides immediately
after the sections melted onto them, and now some of the tissue is hanging
by a thread onto the slide, some has dislodged entirely, and some remains
adherent.

Any helpful hints out there?

Thanks,

Dotty




From daemon Tue Nov 5 13:10:46 2002



From: Jeff Stewart :      jeff-at-metallography.com
Date: Tue, 05 Nov 2002 13:59:37 -0500
Subject: Re: San Antonio 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Also see http://www.msa.microscopy.com/MSAMeetings/MM03/MMHomePage.html

Jeff Stewart
Metallographic Laboratory Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703

"Dwight Arrieche (IIBCA)" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Microscopist
}
} I am looking for some information on the next meeting listed below, but
} could not find a link at
} http://www.msa.microscopy.com/MSAMeetings/MSAMeetings.html
}
} Microscopy & Microanalysis 2003
}
} Sponsored by MSA, MAS, and CIASEM
}
} August 3-7 in San Antonio Texas.
}
} Greatly appreciate your help



From daemon Tue Nov 5 13:28:54 2002



From: Frank Thomas :      thomasf-at-gsca.NRCan.gc.ca
Date: Tue, 5 Nov 2002 15:17:20 -0400
Subject: Re: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is the "Particle Atlas" produced by the McCrone Institute. I've never
seen it, and I hear it's quite expensive, but as I understand it, it
contains many SEM images of common particles and materials.
I'm not sure of the exact name, but if you go to the McCrone Institute
website, it should be described there.

F.C. Thomas
Geological Survey of Canada (Atlantic)
Dartmouth, Nova Scotia



From daemon Tue Nov 5 14:17:45 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 5 Nov 2002 14:04:07 -0600
Subject: Re: TEM: Immuno Staining / Negative Staining of virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Thomas,
One suggestion would be to cut down on the washes. You could
try adding the primary and then after the incubation simply adding
the secondary right on top of that. Then you just need one wash, not
two. This might increase your background but perhaps not too bad.
Another thing you can try is checking out the washing solution. Are
you using PBS to wash? try diluting it or even increasing the ionic
strength. Some funny things happen with stickiness and salt
concentration.

Hope this helps,
Tobias

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Tue Nov 5 15:47:22 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 05 Nov 2002 16:33:12 -0500
Subject: TEM prep of diamond anvil produced 50 um particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ian MacLaren wrote:
========================================================
Has anyone ever prepared TEM specimens from small samples of the size
10-100 microns. We have a student who is studying phase transitions in
a diamond anvil cell and wants to put the samples in the TEM. The
resulting samples are usually of the order of 50 microns in diameter,
and rather thinner in cross section.
This falls in an inconvenient size range: I normally work with either
bulk materials which can be thinned, or with nanoscale particles which
can be dispersed in something and dropped onto a holey carbon film.
I was wondering if there was perhaps some way of embedding the samples
and then thinning the resulting composite.
The material is a hard glassy or crystalline ceramic, depending on the
exact treatment in the diamond anvil cell.
===========================================================
We have been embedding samples in this size range, on and off for more than
thirty years.

Actually they are quite easy to handle, at least with practice. I think
that some of the catalyst samples we have thin sectioned, such as zeolites,
would fall into the size range you mentioned. We have (of course) a
preference for our own SPI-Pon™ 812 Epoxy Resin system, but at least some of
the "Epon substitutes" offered by our major competitors should work just as
well. The catalyst samples have some internal porosity so vacuum
impregnation is standard. But that might not be necessary if the smaples
were diamond anvil pressed and dense.

We use our own SPI Materials Science Diamond Knives, 45 deg., and generally
have not had any particular problems. The smaller the particles, the better
the sections. They might not be perfect, but they should show you the
structure and morphology of the particles. We have found that the 55 deg.
knives tend to pull particles out of the block because of compression
effects.

Disclaimer: SPI Suppplies is a source for embedding resins and mateials
science diamond knives so we have a vested interest in the promotion of
these products.

Chuck

PS: Remember that we are striving to be 100% paperless, therefore there
are no paper copies kept of this correspondence. Please be sure to always
reply by way of "reply" on your software so that the entire string of
correspondence can be kept in one place.
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Tue Nov 5 16:05:02 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 5 Nov 2002 13:56:49 -0800
Subject: TEM Sample prep: 10-100 micron scale samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian;
A dual beam FIB equipped with an Omniprobe micromanipulator should do the job.

John Mardinly
Intel



-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Monday, November 04, 2002 7:36 AM
To: Microscopy


Dear all,
Has anyone ever prepared TEM specimens from small samples of the size
10-100 microns. We have a student who is studying phase transitions in
a diamond anvil cell and wants to put the samples in the TEM. The
resulting samples are usually of the order of 50 microns in diameter,
and rather thinner in cross section.
This falls in an inconvenient size range: I normally work with either
bulk materials which can be thinned, or with nanoscale particles which
can be dispersed in something and dropped onto a holey carbon film.
I was wondering if there was perhaps some way of embedding the samples
and then thinning the resulting composite.
The material is a hard glassy or crystalline ceramic, depending on the
exact treatment in the diamond anvil cell.

Thanks for any leads you can give.

Best wishes
--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Tue Nov 5 16:13:44 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 5 Nov 2002 17:05:45 -0500
Subject: TEM Sample prep: 10-100 micron scale samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ron Anderson and company presented work a few years ago where they put small sand-sized pieces of material into holes in Si that they put there by using a special rounded-solid tool for an ultrasonic drill that scalloped the round hole. They then covered the sample with epoxy and another piece of Si and used their tripod polishing to prepare the sample normally. A modification of this theme would probably work for you. Of course there is always FIB if you can get access to one.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Monday, November 04, 2002 10:36 AM
To: Microscopy


Dear all,
Has anyone ever prepared TEM specimens from small samples of the size
10-100 microns. We have a student who is studying phase transitions in
a diamond anvil cell and wants to put the samples in the TEM. The
resulting samples are usually of the order of 50 microns in diameter,
and rather thinner in cross section.
This falls in an inconvenient size range: I normally work with either
bulk materials which can be thinned, or with nanoscale particles which
can be dispersed in something and dropped onto a holey carbon film.
I was wondering if there was perhaps some way of embedding the samples
and then thinning the resulting composite.
The material is a hard glassy or crystalline ceramic, depending on the
exact treatment in the diamond anvil cell.

Thanks for any leads you can give.

Best wishes
--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Tue Nov 5 18:21:24 2002



From: MicroscopyToday :      microtod-at-optonline.net
Date: Tue, 05 Nov 2002 19:06:25 -0500
Subject: Re: San Antonio 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The M&M-2003 Call for Papers is printed and will be mailed along with
the November issue of Microscopy Today and the M&M-2003 poster in about
two to three weeks. Nestor, the MSA Listserver friendly SysOp. Has the
Call for Papers files and will post them as soon as his schedule
permits, probably in the next week.

If anyone URGENTLY needs any information from the Call for Papers, they
can contact me. Or, you can wait a little bit. :-)

Ron Anderson
Microscopy Today and Call for Papers Editor

-----Original Message-----
} From: Jeff Stewart [mailto:jeff-at-metallography.com]
Sent: Tuesday, November 05, 2002 11:33 AM
To: Dwight Arrieche (IIBCA)
Cc: microscopy-at-sparc5.microscopy.com


Dwight,

I have some information posted at my website regarding the Call for
Papers
etc. for the San Antonio meeting which might be helpful although it's
from
an International Metallographic Society (one of the participating
organizations) perspective. Take a look at
http://www.metallography.com/ims/2003call.htm and
http://www.metallography.com/ims/2003.htm.

Jeff Stewart
Metallographic Laboratory Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703


"Dwight Arrieche (IIBCA)" wrote:

}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
} Dear Microscopist
}
} I am looking for some information on the next meeting listed below,
but
} could not find a link at
} http://www.msa.microscopy.com/MSAMeetings/MSAMeetings.html
}
} Microscopy & Microanalysis 2003
}
} Sponsored by MSA, MAS, and CIASEM
}
} August 3-7 in San Antonio Texas.
}
} Greatly appreciate your help





From daemon Wed Nov 6 01:45:36 2002



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Wed, 06 Nov 2002 08:36:59 +0100
Subject: Re: pre-embedding immunogold TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Why are you using gelatin on Superfrost plus slides - I thought the point
of S'frost was that they 'did the business' - or have I missed something?
Why not just use the S'fost on their own?

I would be interested in comments.

Gareth


At 12:06 2002-11-05 -0500, Dorothy Sorenson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Obs/NB New postal/visiting address from July 2002!

Med vänliga hälsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 5858 1038
Fax +46 728 3747

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Huddinge Universitetssjukhus, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.



From daemon Wed Nov 6 01:51:34 2002



From: cameron_hind-at-baxter.com
Date: Wed, 6 Nov 2002 08:43:16 +0100
Subject: Re:EM atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hong,

An excellent book/atlas:

Cell Structure; an Introduction to Biomedical Electron Microscopy

Churchill Livingstone ISBN 0 443 02324 7

My 3rd edition dates from 1982 but is still nevertheless a very good book
and even has sections on techniques and applications as well as being an
atlas with good descriptions,

check it out,

Cameron Hind
Analytical Resources group
Baxter R&D Europe
Belgium





From daemon Wed Nov 6 05:55:33 2002



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Wed, 06 Nov 2002 12:47:15 +0100
Subject: Re: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Histology: A text and atlas takes a lot of beating - it goes from LM
through to EM. It is out of print now but Amazon.com still has some copies
available.

Gareth



At 15:17 2002-11-05 -0400, Frank Thomas wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Obs/NB New postal/visiting address from July 2002!

Med vänliga hälsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 5858 1038
Fax +46 728 3747

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Huddinge Universitetssjukhus, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.



From daemon Wed Nov 6 08:23:28 2002



From: Julian Smith III :      smithj-at-Winthrop.edu
Date: Wed, 6 Nov 2002 09:14:10 -0500
Subject: Re: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you mean Ross, Romrell, and Kaye, it is just leaving its third
edition, with a new edition due out this December (which is why, I
guess, the third is now "out of print"). Great book for a histology
course. Wouldn't necessarily be my first choice for an EM atlas,
though.
JSIII

} Histology: A text and atlas takes a lot of beating - it goes from LM
} through to EM. It is out of print now but Amazon.com still has some
} copies available.
}
} Gareth
}
}
--
Julian P.S. Smith III
Dept. of Biology
Winthrop University
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)


From daemon Wed Nov 6 08:49:48 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Wed, 6 Nov 2002 08:41:37 -0600
Subject: RE: SEM/EDS - bacteria on limestone, etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hi group - not owning a critical point dryer, etc., what would be the
} best solution in preparing a fresh core sample from fresh
} water so that
} the bacteria will not become desiccated? I do have opportunity to run
} SEM on low vacuum which will help.
} Thanks
} Barb

Do you have a cooling Peltier stage?
Low vacuum by far is not enough to keep specimens wet.
If you do not have real wet mode (ESEM), then better
to fix specimens in gluteraldehyde (2%) for 0.5 - 1 hr, dehydrate
in graded alcohol (for example, 30,50,75,96 and 100%
for 10 min each), and then place in HMDS (hexamethyldesilazane)
for 20 min and air dry for 1 hr. With HMDS you do not need to
use critical point dryer and usually it works well for
bacteria. In many cases you even do not need absolute (100%)
alcohol, 96% often works fine as a final step in dehydration.


From daemon Wed Nov 6 09:33:39 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Wed, 6 Nov 2002 10:24:44 -0500
Subject: Plant/Botanical EM books

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Listers:
}
} I have recently had several requests to process and embedd plant
} tissue. All of my previous EM experience has involved only human and
} animal tissue.
}
} Those of you who routinely do botanical specimens can you recommend
} some EM books or atlases which cover processing methods?
}
} Are there any atlases that provide ultrastructural images so I can
} learn the morphology/terminology of different types of plant specimens.
} Right now I will be looking at potato leaves and tubers. Later I am
} supposed to get a tomato for EM processing.
}
} Thanks for your help!
}
} Karen L. Bentley, M.S.(previously Jensen)
} Associate Scientist & Project Manager
} Electron Microscope Research Core
} University of Rochester Medical Center
} Rochester, NY 14642
} 585-275-1954
}


From daemon Wed Nov 6 09:45:56 2002



From: Lauren :      simmerman_2000-at-netzero.com
Date: Wed, 6 Nov 2002 15:38:28 GMT
Subject: TEM- Pinholes in tissue?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I have a quick question. Does anything besides water cause small pinholes
throughout tissue?
Specifically, we had a fairly large piece of brain tissue 3cmx1cmx.5cm. We cut out small cubes from this. The large piece was originally formalin fixed and the cubes were post fixed in MPG. The sections on the grid were full of tiny holes. If my memory serves me right, we have had this problem before with brain tissue? Any connection?
A fresh pint of absolute ETOH was used, and we are always very careful to keep all water out of processing.

Thank You,
Lauren Simmerman
Nebraska Health System EM Lab






From daemon Wed Nov 6 09:50:09 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Wed, 6 Nov 2002 10:42:24 -0500
Subject: TEM: Immuno Staining / Negative Staining of virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tom:

Here is a good method from Hsiung's Diagnostic Virology Book, page 102 (4th
ed.).

1)Mix Virus suspension with homologous antiserum at predetermined dilution.

2)Incubate mixture -at-37C for 1 hour or overnight -at-4C

3)Centrifuge virus-antibody mixture at 15,000xg for 30 min.

4)Resuspend pellet with small amount of dh20

5)Apply suspension of virus-antibody complexes to a formvar coated nickel
grid and remove the excess by blotting with a piece of filter paper.
Immediately stain with 2% PTA for 1 minute (avoid drying during
preparation).

6)Examine grid under EM.

Good Luck!

Karen Bentley

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954








-----Original Message-----
} From: Thomas Weber [mailto:thomas.weber-at-mssm.edu]
Sent: Tuesday, November 05, 2002 10:44 AM
To: Microscopy-at-sparc5.microscopy.com


Hi,
I'm trying to immuno gold stain adeno-associated virus adsorbed to
parlodion coated copper grids. I UV-irradiate the grids for 30 min. to
help the virus stick. If I just uranyl acetate stain the sample, I see
plenty of viral particles. However, after the immuno staining
procedure (1: Wash, 2: primary AB, 3: Wash, 4: secondary AB, 5: wash,
6: uranyl acetate staining) I can't detect any virus particles anymore.
All I see is low background staining with immuno gold.

I would appreciate any suggestions. Is there any way to crosslink the
virus to the grid? Or would fixing with Glutaraldehyde before or after
adsorption help?

I'm not a regular visitor to this list, I would appreciate if you could
e-mail me a copy of the answer directly to: thomas.weber-at-mssm.edu.


Thanks for any help.


Thomas

P.S.: I don't have really access to a glow discharge unit.



From daemon Wed Nov 6 10:01:00 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Wed, 6 Nov 2002 10:52:33 -0500
Subject: pre-embedding immunogold TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dotty:

You can try cutting a thicker frozen section, say around 40-50 microns and
place them into PBS filled wells for labeling as floating sections. We use
a brush to transfer sections during the immunolabeling procedure. If you
want the procedure just let me know and I'll send it to you.

I assume you have cryoprotected the tissue prior to freezing? Perhaps the
the sucrose is causing them to not adhere to the gelatin.

We never use gelatin or anything else on top of the superfrost plus slides.
Those types of slides have a charge on them which is supposed to hold the
tissue without the need of other types of coatings. So I would try the
sections on the naked superfrost plus slides.

Karen

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954


-----Original Message-----
} From: Dorothy Sorenson [mailto:dsoren-at-umich.edu]
Sent: Tuesday, November 05, 2002 12:06 PM
To: microscopy-at-sparc5.microscopy.com


Dear Listers,

I am trying to do pre-embedding ultrasmall-gold immunolabeling of mouse
optic nerve. I am trying to do it on 10 um-thick frozen sections attached
to gelatin-coated superfrost/plus glass microscope slides. I am in the
middle of the first run on these samples, and I am finding that my first
challenge is to get the tissue to stick to the slides. For fluorescence
immuno-labeling, one can allow the frozen sections to dry onto the slides.
For TEM, though, the tissue can?t dry out. I froze the slides immediately
after the sections melted onto them, and now some of the tissue is hanging
by a thread onto the slide, some has dislodged entirely, and some remains
adherent.

Any helpful hints out there?

Thanks,

Dotty




From daemon Wed Nov 6 10:37:03 2002



From: Lauren :      simmerman_2000-at-netzero.com
Date: Wed, 6 Nov 2002 16:26:36 GMT
Subject: TEM- Pinholes in Tissue?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I just wanted to add something to the previous post.
The tissue was from a tumor.






From daemon Wed Nov 6 11:40:56 2002



From: Fang Mei :      fm46-at-cornell.edu
Date: Wed, 6 Nov 2002 13:34:23 -0500
Subject: Re: Fw: TEM neg scanners: Can't get through

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,
I am posting this message for Robert in England, since his Hotmail account
does not seem to be able to access the Listserver.
----- Original Message -----
} From: "Robert H. Olley" {hinmeigeng-at-hotmail.com}
To: {mager-at-interchange.ubc.ca}
Cc: {R.H.Olley-at-reading.ac.uk} ; {hinmeigeng-at-hotmail.com}
Sent: Wednesday, November 06, 2002 12:59 AM


Yes, you can. You do not need any holder for the TEM scanner (but you
do need a negative adapter, not just a regular scanner). Just put it
down on the surface. good luck.

Fang

}
} }
} } Does anyone have experience of Epson scanners for TEM negatives? I have
} } enquired of the company whether the Epson Perfection 2450 Photo can take
} our
} }
} } 4 x 3-and-a quarter inch TEM plates,
} }
} } but all I can get is the standard answer "we have film holders", which
} } include 120 roll film and 5 x 4 inch, but not the size we want. Can one
} } simply place the negative straight down on the scanner surface?
} }
} } Any help would be much appreciated,
} }
} } (Sorry if you've received this message before - I've had one or two failed
} } attempts at sending).
} }
} } -----------------------------------
} } Robert H. Olley, Physics Dept,
} } Univ. Reading, RG6 6AF, England.
} } E-mail: R.H.Olley-at-reading.ac.uk
} } URL: http://www.rdg.ac.uk/~spsolley
} } -----------------------------------
} }
} }
} } _________________________________________________________________
} } STOP MORE SPAM with the new MSN 8 and get 2 months FREE*
} } http://join.msn.com/?page=features/junkmail
} }
} Mary Mager
} Electron Microscopist
} Metals and Materials Engineering
} University of British Columbia
} 6350 Stores Road
} Vancouver, B.C. V6T 1Z4
} CANADA
} tel: 604-822-5648
} e-mail: mager-at-interchange.ubc.ca


--
------------------
Department of MSE
Cornell University
Ithaca, NY14853
(607)-2559461
------------------


From daemon Wed Nov 6 13:25:17 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 6 Nov 2002 13:16:38 -0600
Subject: Re: Fw: TEM neg scanners: Can't get through

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Placing the negatives directly on the scanner plate will often result
in Newton rings in the resulting image. keeping it just off the
glass prevents this. We put our 4 x 3.25 negatives in the 5 x 4
holders where they are supported on 3 sides and this works well.
good luck. tom



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Nov 6 13:27:11 2002



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 06 Nov 2002 14:19:07 -0800
Subject: Re: Plant/Botanical EM books

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,
The following may still be available--

Hall, J.L. and C. Hawes, 1991 Electron Microscopy of Plant Cells, Academic
Press, San Diego
Rosemary



From daemon Wed Nov 6 13:33:15 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 06 Nov 2002 13:25:27 -0600
Subject: Re: Fw: TEM neg scanners: Can't get through

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In Nestor's absence, let me stick my nose into the matter.

I ran into a similar situation a few weeks back. I replied to a poster's
message someone-at-yahoo.com and copied the list. The message never came
through and I think I got a notification back from the list that the
message was blocked as potential SPAM.

What had happened was that the filter triggered on yahoo.com in the address
headers. Apparently the rules are set so that yahoo or hotmail appearing in
the headers is taken as a sign of SPAM. Many spammers will have multiple
addresses in the headers and will likely include plenty of Yahoo and Hotmail.

Strictly speaking, mail really intended for the list should not have to be
copied to multiple addresses, including ones at yahoo or hotmail. You might
say "well, why can't I?" I suppose it could be allowed, but I expect that
the rule does stop a number of SPAM messages. I would allow Nestor the
prerogative to set the rules as he sees fit. It seems he is doing a pretty
good job of catching SPAM. A little gets through, but I am certain many
more messages do not.

If you still want to copy yourself or any other address at Yahoo or
Hotmail, there is a simple solution. Simply put that address in the BCC:,
blind carbon copy, field. That way the list server would not even see the
address to choke on it. This practice is also good for other applications
where messages are going out to many recipients and it is not necessary for
the recipients to see the pages and pages of other recipient addresses.
When I receive a message intended for the engineering faculty and staff, it
really isn't necessary for me to see hundreds of addresses before I get
down to a three-line message. {g}

Try it and see if that helps.

Warren

At 09:29 AM 11/6/02 -0800, you wrote:

} Dear List,
} I am posting this message for Robert in England, since his Hotmail account
} does not seem to be able to access the Listserver.
}
} ----- Original Message -----
} } From: "Robert H. Olley" {hinmeigeng-at-hotmail.com}
} To: {mager-at-interchange.ubc.ca}
} Cc: {R.H.Olley-at-reading.ac.uk} ; {hinmeigeng-at-hotmail.com}
} Sent: Wednesday, November 06, 2002 12:59 AM
} Subject: TEM neg scanners: Can't get through
} }
} } Dear Mary,
} }
} } I've recently rejoined the Micro Listserver, using the hotmail acount in
} the
} } cc: line in order to keep the correspondence from getting mixed up with
} the
} } rest of my university email. While I receive all (?) of the other items
} } posted to the Micro group, none of my own messages get through: also
} } attempts to contact Nestor seem to fail. I don't know if for some reason
} } I'm getting "spammed out", but I would appreciate it if you could post
} this
} } enquiry to the listserver and see what happens; please also cc: to myself
} at
} } both addresses above.
} }
} } Best regards,
} }
} } Robert H. Olley.

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Wed Nov 6 13:55:26 2002



From: Ann-Fook Yang :      yanga-at-agr.gc.ca
Date: Wed, 06 Nov 2002 14:45:47 -0500
Subject: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

I have two SEM micrographs on the web:
http://www.magma.ca/~scimat/Defect.htm
The micrographs show zagged edges taken at 20 kX. Such phenomenon does not present all the time. Can anyone suggest what causes the problem and how to solve it?
Thanking in advance.









AnnFook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Room 2091, Bldg. 20,
Central Experimental Farm,
Ottawa, Ontario
Canada K1A 0C6

Tel: 1-613-759-1638
Fax: 1-613-759-1701

e-mail: yanga-at-em.agr.ca



From daemon Wed Nov 6 14:03:15 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 06 Nov 2002 14:55:40 -0500
Subject: B & W film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


** Reply Requested When Convenient **

Hi listers,

I'm looking for a black and white reversal film. I have some old LPD4
but I can't kind listed anywhere (fuji or kodak) if they still make this
film. And if they do make it, does it come in little rolls or for a
bulk loader?

I'm lazy and I like to make B&W text slides with B&W reversal film. Or
can I use a color slide film and still have it turn out OK?

Any information is gladly accepted and I thank you in advance.

Paula :-)

p.s. some of us are old fashioned and like to use film instead of all
the fancy-schmancy computer thingies ;)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax


From daemon Wed Nov 6 14:07:06 2002



From: Leslie Cummins :      gunther-at-aecom.yu.edu
Date: Wed, 06 Nov 2002 15:05:55 -0500
Subject: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listers

As Kodak slowly discontinues the graded Kodabrome II RC papers (no 1, 4 or
5), we are looking for alternatives for our printing.

We have a Durst point source enlarger, and I was wondering if we can switch
to Polycontrast paper and filters. If there are any labs that are using
this method, I would appreciate any information on how well it works.

We have found that it is still more economical and time efficient to
develop and print 8 x 10 study prints for our users, rather than scan in
all our negatives, so any old-fashioned wet darkroom suggestions would be
appreciated.

Thanks in advance
Leslie


Leslie Gunther Cummins
Analytical Imaging Facility
Albert Einstein College of Medicine
1300 Morris Park Ave.
Bronx, NY 10461
718-430-3547

http://www.aecom.yu.edu/aif/



From daemon Wed Nov 6 15:13:52 2002



From: Berg, R. Howard :      RHBerg-at-danforthcenter.org
Date: Wed, 6 Nov 2002 15:06:13 -0600
Subject: Re: Plant/Botanical EM books

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,

There is an excellent LM/EM atlas available from Jones and Bartlett
Publishers (Subdury, MA, 800-832-0034):

"Plant cell biology: structure and function", by B.E.S. Gunning and M.W.
Steer
paperback ISBN 0-86720-504-0, the edition I have is from 1996

And there is a recent plant-oriented EM technique book:

"Methods in plant electron microscopy and cytochemistry", edited by W.V.
Dashek (Humana Press 973-256-1699), ISBN 0-89603-589-1 (2000)

Hope this helps.

Howard




} Dear Listers:
}
} I have recently had several requests to process and embedd plant
} tissue. All of my previous EM experience has involved only human and
} animal tissue.
}
} Those of you who routinely do botanical specimens can you recommend
} some EM books or atlases which cover processing methods?
}
} Are there any atlases that provide ultrastructural images so I can
} learn the morphology/terminology of different types of plant specimens.
} Right now I will be looking at potato leaves and tubers. Later I am
} supposed to get a tomato for EM processing.
}
} Thanks for your help!
}
} Karen L. Bentley, M.S.(previously Jensen)
} Associate Scientist & Project Manager
} Electron Microscope Research Core
} University of Rochester Medical Center
} Rochester, NY 14642
} 585-275-1954
}
}
}
R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility
Danforth Plant Science Center
975 N. Warson Rd.
St. Louis, MO 63132

ph 314-587-1261 fx 314-587-1361 cell 314-378-2409
rhberg-at-danforthcenter.org www.danforthcenter.org


From daemon Wed Nov 6 15:13:54 2002



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Wed, 06 Nov 2002 15:48:57 -0700
Subject: Cryosectioning/mouse heart

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We've been struggling trying to cut cryo-sections of mouse hearts.
The samples have ben fixed with PFA (4-8%) by perfusion, then
sliced into thin ( { 1mm) sections, chopped and infiltrated in 2.3 M
sucrose ON before freezing in LN2. Sections are cut at -108°C,
with either 35 or 45° cryo-knife, and picked with methyl cellulose -
sucrose mix (50::50 or 60::40). The problem is we get too much
compression of the sample and too many folds. These folds occur
mostly over the sarcolemma, which is unfortunately the area where
we try to see labeling! I suspect one of the problems might be the
infiltration medium we use (2.3 M sucrose), or the fixation. We
have tried to add some glutaraldehyde (0.1%) without success.
I would greatly appreciate any suggestion on how to improve our
preservation and sectioning of these samples. Thanks

Marc
--
Marc Pypaert
Department of Cell Biology,
Center for Cell and Molecular Imaging and
Ludwig Institute for Cancer Research,
Yale University School of Medicine
333 Cedar Street
New Haven CT 06520
Tel : (203) 785 3681
Fax : (203) 785 7446




From daemon Wed Nov 6 16:33:58 2002



From: Sharon Drew :      drewsh-at-musc.edu (by way of MicroscopyListserver)
Date: Wed, 6 Nov 2002 16:26:56 -0600
Subject: Clinical turn around

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!
I run a diagnostic TEM laboratory in SC.
One tech.
6 to 5 samples every other day at least.
What is the turn around time asked for your labs out there that are
also doing diagnostic/clinical work and where does that number come
from?
Thank you for any help.
My supervisor is saying 2 days but I think that is close to impossible
when only one tech doing everything including transport of tissue from
another lab and scoping all case that come in.
Sharon Drew
Diagnostic Pathology EM
Charleston, SC


From daemon Wed Nov 6 16:35:15 2002



From: Allen Sampson :      ars-at-sem.com
Date: Wed, 6 Nov 2002 17:41:00 -0800
Subject: RE: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: {mailto:jsmit51-at-tampabay.rr.com} jerry smith
To: {mailto:MICROSCOPY-at-MSA.MICROSCOPY.COM} MICROSCOPY-at-MSA.MICROSCOPY.COM
Sent: Wednesday, November 06, 2002 11:45 AM


Yup, that's vibrations. As you've found EMs are extremely sensitive
seismometers.

Ridding an SEM of such problems can be as much art as science. It's always
nice to know the source but they are often created by many activities or
sources in a building. I've seen them caused by high speed tanks (a
location next to United Technologies M1A1 Abrams tank test track), ocean
waves (Pensacola Naval Air Station) and often by nearby heavy vehicle
traffic. Most likely, you'll primarily have a combination of vibrations
caused by building air handling equipment.

Primarily, the instrument has to be properly setup. There are two or three
'zones' of isolation that have to be paid attention to. The inner most
zone, and the one that should have the least vibrations, is the column and
sample chamber. This is actually isolated from the electron optics table,
usually by elastomeric shock mounts. You'll want to make sure those are in
good shape. Ideally, nothing should make any mechanical contact between
this inner table and the outside world. However, that's obviously not
possible - there are many wires and tubes that have to connect to the
components on the inner table, both above and below the sample chamber.
And never overlook the little things, like that clipboard or instrument
log that's always left on the optics table, sometimes touching the chamber.

The main idea is to make sure that anything that does have to go to the
inner table is first secured to the outer table. In the case of wires and
tubing, they should be secured in such a way that there is a loose loop
formed between the attachment to the inner and outer tables that touches
nothing else. The loop (really a U shape) helps to re-direct vibrations to
a swing in the wire or cable. The outer table, itself, has to also be
considered an isolated zone, albeit with less isolation than the inner
table. The mass of the entire electron optics table and its rigidity while
only being supported by four thin feet, gives it some isolation even though
it is just sitting on the floor. So all wires and tubing that go from the
outer table to other things should also be similarly routed. It's usually
not possible to loop these to the electronics console or water and
electrical outlets, so sometimes sand or lead bags can be used to dampen
vibration in them before they loop to the optics table. Bags of lead shot
are cheap and easy to get at many sporting stores (used for loading your
own shotgun shells).

Adding an EDS detector adds to the problem. The long moment arm formed by
the length of the mounting means that any vibrations coming through the
electrical connections to the end of the detector will be easily coupled
into the column. If possible, secure them to the inner table and then the
outer table. Another vexing problem with EDS detectors is their ability to
couple acoustic noise from their large cryogenic dewars. Many have this
problem - try clapping your hands loudly when you're looking at an image,
if your EDS is susceptible to acoustics, you'll see a decaying vibration
from the clap. Best I've come up with if you have this problem is to wrap
the dewar with some sound deadening foam, this will help stop the
vibrations from reaching the dewar and dampen any that do.

Good Luck!


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, November 06, 2002 11:46 AM, Ann-Fook Yang
[SMTP:yanga-at-agr.gc.ca] wrote:
} Hi everyone,
}
} I have two SEM micrographs on the web:
} http://www.magma.ca/~scimat/Defect.htm
} The micrographs show zagged edges taken at 20 kX. Such phenomenon does
not present all the time. Can anyone suggest what causes the problem and
how to solve it?
} Thanking in advance.
}
}
}
}
}
}
}
}
}
} AnnFook Yang
} EM Unit,
} Eastern Cereal and Oilseed Research Centre,
} Room 2091, Bldg. 20,
} Central Experimental Farm,
} Ottawa, Ontario
} Canada K1A 0C6
}
} Tel: 1-613-759-1638
} Fax: 1-613-759-1701
}
} e-mail: yanga-at-em.agr.ca
}
}
}
}



From daemon Wed Nov 6 19:47:09 2002



From: Benjamin - Simkin :      simkin-at-egr.msu.edu
Date: Wed, 6 Nov 2002 20:37:24 -0500 (EST)
Subject: Re: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ann, I agree with Allen Sampson's comments, with the addition that you could
also get the same effect from AC magnetic fields. These can pop up from a
number of sources (computer equipment, electroc motors, overhead lights, the
list goes on...).
A way of checking if it's vibration as opposed to fields is to check the
magnitude of the 'sawteeth' at long and short working distances; if it's fields,
then the sawteeth will be bigger at longer working distances (because the action
of the field will be operating over a longer distance at the longer working
distance). This also serves as a crude short-term fix for the problem sometimes,
although I'd really recommend finding the piece of equipment that's causing
the problem, and fix that instead.
Another possible source is a ground loop, where your 'scope is hooked up to
another piece of equipment with a differant ground potential. This causes
currents to end up flowing through your equipment-'scope connection, generating
the AC fields internally. A solution for this is to decide on a single
ground potential to use (usually the 'scope's dedicated ground), and then
hardwire all the other equipment to this one ground. (The best case, of course,
is to sink your own ground, but many people/labs don't have this option
available.)
If you do have field problems, you can usually track them down using an
AC magnetic field hand meter, which you can get for around $35 from an
online dealer (I forget where we got ours now, sorry).

I hope this helps,

Ben Simkin (simkin-at-egr.msu.edu)
Michigan State University
Department of Chemical Engineering and Materials Science


From daemon Wed Nov 6 20:00:45 2002



From: saram-at-duke.edu
Date: Wed, 6 Nov 2002 20:46:06 -0500 (EST)
Subject: Re: Clinical turn around

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Sharon,

Our turn around time is about 2 days, but we have 2 FTE's doing ~500 Surg
Path EM specimens/yr, (that's about one full case/day). Also, my virology
EM techs and I can fill in when the SPEM service gets bombed. The TAT
will vary more when there's only one person to do everything. When
there's a pile up with several samples at once and no back-up tech,
obviously, the TAT has to increase. I would think 2-4 days is
reasonable, depending on the work flow.

What do you do if you're out sick or on vacation? This supervisor needs
to lighten up a little and realize that one person can't have a set TAT
unless the cases arrive in a regular and uniform flow--which isn't going
to happen.

You might set up some sort of system where the pathologist lets you know
that a case is really urgent and put that one in front of others. You
can do a better job if you communicate clearly with the folks needing the
service. You can give them an approximate, estimated, or usual TAT to
expect and then notify them if it must be longer because of whatever
(workload, illness, equipment malfunction, vacation, etc). Learn to work
with those needing the service and build a good report; then have them
back you up wrt the supervisor.

..my $0.02 worth.

Best regards and greetings to "Whit"
Sara Miller


On Wed, 6 Nov 2002, Sharon Drew wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi!
} I run a diagnostic TEM laboratory in SC.
} One tech.
} 6 to 5 samples every other day at least.
} What is the turn around time asked for your labs out there that are
} also doing diagnostic/clinical work and where does that number come
} from?
} Thank you for any help.
} My supervisor is saying 2 days but I think that is close to impossible
} when only one tech doing everything including transport of tissue from
} another lab and scoping all case that come in.
} Sharon Drew
} Diagnostic Pathology EM
} Charleston, SC
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Wed Nov 6 20:42:37 2002



From: Thor Bostrom :      t.bostrom-at-qut.edu.au
Date: Thu, 07 Nov 2002 12:46:07 +1000
Subject: Fw: TEM neg scanners & 16-bit images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Robert and listers,

We have a recently acquired Epson Perfection 2450 Photo scanner and it
works very well with TEM negs mounted in the 5 x 4 film holder. You want to
avoid placing the negs directly on the glass surface because of Moire
fringes. (Curiously we had little trouble with fringes on our old HP
transparency scanner.)

The Epson scanner can acquire 16-bit grayscale TIFF images which can be
advantageous for negatives with very low contrast. However few software
packages seem to handle this format. DigitalMicrograph and analySIS do,
while Adobe Photoshop provides some limited support. Are there other
programs out there that can work with this format?

With thanks,
Thor

} = = = = = = = = = = = = = = = = = = =
}
} Does anyone have experience of Epson scanners for TEM negatives? I have
} enquired of the company whether the Epson Perfection 2450 Photo can take
} our 4 x 3-and-a quarter inch TEM plates,
} but all I can get is the standard answer "we have film holders", which
} include 120 roll film and 5 x 4 inch, but not the size we want. Can one
} simply place the negative straight down on the scanner surface?
}
} Any help would be much appreciated,
}
} (Sorry if you've received this message before - I've had one or two failed
} attempts at sending).
} -----------------------------------
} Robert H. Olley, Physics Dept,
} Univ. Reading, RG6 6AF, England.
} E-mail: R.H.Olley-at-reading.ac.uk
} URL: http://www.rdg.ac.uk/~spsolley
} -----------------------------------
}
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Dr Thor Bostrom
Acting Director,
Analytical EM Facility,
Faculty of Science,
Queensland University of Technology (QUT)
GPO Box 2434, Brisbane, QLD 4001, Australia
Ph: +61 7 3864-2351 FAX: +61 7 3864-5100
http://www.sci.qut.edu.au/aemf/
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=



From daemon Wed Nov 6 22:01:00 2002



From: =?iso-8859-1?q?Ike=20Oguocha?= :      oguocha-at-yahoo.com
Date: Thu, 7 Nov 2002 03:52:07 +0000 (GMT)
Subject: Re: Fw: TEM neg scanners: Can't get through

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Robert:

Unless there's something special an Epson scanner does better than
other scanners, you really don't need an Epson scanner to manipulate
both SEM and TEM negatives. I may be wrong but all you need are a
good scanner and a suite of Adobe Photoshop or PaintShop Pro.

All we do is to scan our negatives into either Paintshop Pro or
Photoshop. In Paint Shop Pro, for ordinary BF/DF negtaives, I just
go to the "Color" menu and select "Negative Image". And my scanned
image turns to a "print image". No wasting of developers and fixers.
You won't be disappointed with what you would get. Just get a good
scanner. We have also got good quality diff images. Of course, if
you know how to use the Fourier Transform filter in your graphic
suite, you can still do a lot using your Photoshop or Paint Shop Pro.
Never have to worry about film holders. We just lay our negatives
flat on our scanner and off we drive.

Well, I don't know if this comes close to what you are looking for.

Goodluck.

Ike Oguocha

} Does anyone have experience of Epson scanners for TEM negatives? I
} have enquired of the company whether the Epson Perfection 2450 Photo
} can take our
} }
} } 4 x 3-and-a quarter inch TEM plates,
} }
} } but all I can get is the standard answer "we have film holders",
} which
} } include 120 roll film and 5 x 4 inch, but not the size we want.
} Can one
} } simply place the negative straight down on the scanner surface?
} }
} } Any help would be much appreciated,
} }
} } (Sorry if you've received this message before - I've had one or two
} failed
} } attempts at sending).


__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com


From daemon Wed Nov 6 22:06:54 2002



From: Anthony Rinaldi :      Anthony_Rinaldi-at-Jabil.com
Date: Wed, 6 Nov 2002 22:59:47 -0500
Subject: ZAF numbers and K ratios

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am using a rather old (almost antique) EDS system for my X ray mapping. It
is so old that it will not do quantitative analysis. With the economy like
it is I will have to make this equipment last a few more years.

Does anyone know of a program that I can use that contains the reference
data tables and allows you to compute the ZAF number (or K ratios ) for
different elements? Doing this by hand is quite tedious. Thank you.

Anthony Rinaldi



From daemon Wed Nov 6 23:08:24 2002



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thu, 7 Nov 2002 00:18:57 -0500
Subject: Fw: vibration?

Contents Retrieved from Microscopy Listserver Archives
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Could be vibration. Could also be magnetic field. And, could be signal
interference, such as ground loop. What type of SEM are you using?

I will omit remedies for the vibration problem, since Allen Sampson did
pretty good job addressing that.

Stray AC line frequency magnetic field: try acquiring image at different
accelerating voltages. It is very important that all other geometry
parameters will remain the same: particular area of a particular specimen,
WD, tilt, magnification, spot size. Contrast, brightness, focus, and
stigmator settings can be changed. If the problem is much worse at, say, 2
kV, than at, say, 15kV, you are dealing with magnetic field. You may also
try to keep 3 axes AC milliGauss meter at the SEM, and notice what it
measures, but catching stray magnetic interference this way may be a little
tricky. These meters, though, are very inexpensive, and available from many
test instruments suppliers.

Now we are getting down to statistically most likely problem- signal
interference and ground loops. It is difficult to go any further without
knowing the SEM type, and acquisition system (if separate) type. Too many
possibilities exist, but the very first- does your acquisition software
have an option somewhere in the settings which reads something like "60
Hertz sync", or "AC line synchronization", or "Line sync", etc., etc.? If
yes, check (or uncheck) that option and see what happens. Further remedies
of this problem will include eliminating ground loops. Example- shielded
cable(s) has shield connected on both sides, and shield is used as one of
the signal wires. This is frequently done, and does jeopardize instrument
interference tolerance. Another example- one of the accessories is
susceptible to some kind of interference, and must be powered through the
isolation transformer. The latter remedy will only work with decent (better
if dedicated) ground. The fact that the problem is not present all the time
does not mean that everything is perfect inside the SEM. Besides, going
after the EM interference source could be inefficient and frustrating, if
not impossible. Improving grounding/shielding/power connection might be
easier.


Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: Ann-Fook Yang {yanga-at-agr.gc.ca}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, November 06, 2002 2:45 PM


Could be vibration. Could also be magnetic field. And, could be signal
interference, such as ground loop. What type of SEM are you using?

I will omit remedies for the vibration problem, since Allen Sampson did
pretty good job addressing that.

Stray AC line frequency magnetic field: try acquiring image at different
accelerating voltages. It is very important that all other geometry
parameters will remain the same: particular area of a particular specimen,
WD, tilt, magnification, spot size. Contrast, brightness, focus, and
stigmator settings can be changed. If the problem is much worse at, say, 2
kV, than at, say, 15kV, you are dealing with magnetic field. You may also
try to keep 3 axes AC milliGauss meter at the SEM, and notice what it
measures, but catching stray magnetic interference this way may be a little
tricky. These meters, though, are very inexpensive, and available from many
test instruments suppliers.

Now we are getting down to statistically most likely problem- signal
interference and ground loops. It is difficult to go any further without
knowing the SEM type, and acquisition system (if separate) type. Too many
possibilities exist, but the very first- does your acquisition software
have an option somewhere in the settings which reads something like "60
Hertz sync", or "AC line synchronization", or "Line sync", etc., etc.? If
yes, check (or uncheck) that option and see what happens. Further remedies
of this problem will include eliminating ground loops. Example- shielded
cable(s) has shield connected on both sides, and shield is used as one of
the signal wires. This is frequently done, and does jeopardize instrument
interference tolerance. Another example- one of the accessories is
susceptible to some kind of interference, and must be powered through the
isolation transformer. The latter remedy will only work with decent (better
if dedicated) ground. The fact that the problem is not present all the time
does not mean that everything is perfect inside the SEM. Besides, going
after the EM interference source could be inefficient and frustrating, if
not impossible. Improving grounding/shielding/power connection might be
easier.


Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: Ann-Fook Yang {yanga-at-agr.gc.ca}
To: {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, November 06, 2002 2:45 PM
} Subject: vibration?
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi everyone,
} }
} } I have two SEM micrographs on the web:
} } http://www.magma.ca/~scimat/Defect.htm
} } The micrographs show zagged edges taken at 20 kX. Such phenomenon does
} not present all the time. Can anyone suggest what causes the problem and
how
} to solve it?
} } Thanking in advance.
} }
} }
} }
} }
} }
} }
} }
} }
} }
} } AnnFook Yang
} } EM Unit,
} } Eastern Cereal and Oilseed Research Centre,
} } Room 2091, Bldg. 20,
} } Central Experimental Farm,
} } Ottawa, Ontario
} } Canada K1A 0C6
} }
} } Tel: 1-613-759-1638
} } Fax: 1-613-759-1701
} }
} } e-mail: yanga-at-em.agr.ca
} }
} }
} }
}
}



From daemon Thu Nov 7 00:02:02 2002



From: paul r hazelton, PhD :      Paul_Hazelton-at-umanitoba.ca
Date: Wed, 06 Nov 2002 23:53:16 -0600
Subject: Re: B & W film

Contents Retrieved from Microscopy Listserver Archives
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try kodalith. you get white on black, very fine resolution. and in the
dark ages i used to hand colour them with photographic tints and a 00000
red fox brush.

computers are really an improvement, though.




From daemon Thu Nov 7 00:23:45 2002



From: paul r hazelton, PhD :      Paul_Hazelton-at-umanitoba.ca
Date: Thu, 07 Nov 2002 00:15:14 -0600
Subject: Re: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
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leslie

i am still using an old ektamatic processor with polycontrast III
professional grade paper. our department of anatomy is also using the
same system. the major problem is now getting parts for the processor,
the rollers tend to go after about 20 years and i have been ordering
replacements on a gradual basis directly from kodak. also, when i
ordered chemicals yesterday our systems contract photographic supplier
suggested that the two labs using this system are out of touch with
reality. polycontrast papers work well. see what kodak now has for a
processor. the Durst enlarger should be fine.

as a hint, one benefit of the polycontrast paper comes if you use a two
setting digital timer. i can set it to expose for two different times,
determine filtration conditions for different areas on the negative, and
do double filter exposures, ensuring good results for publications.
takes a bit of work, but once you have the conditions right you can run
off 10 prints in about 2 minutes. i've personally never seen the
ability to get the same quality from digitized images. but then i'm a
dinosaur.

paul hazelton



From daemon Thu Nov 7 03:09:38 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Thu, 07 Nov 2002 09:58:38 +0100
Subject: Re: Fw: TEM neg scanners: Can't get through

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Dear Robert and all,
We have an Epson 1640SU scanner with film scanning attachment. It came
with 2 holders for negatives, but not the 3 1/4 x 4 inch. However, if
you canabalise the 35 mm holder by taking out the central bar, then it
takes 3 1/4 x 4 perfectly. This is far prefarable to laying the
negative on the glass, since (1) we get no Newton's rings and (2) the
negative is always in the same place, and you can therefore keep all the
scanned area the same for each neg, when scanning a series in, which
saves time on always having to preview, select the area and so on.

I find the Monochrome negative film setting in the software also very
good, and the automatic adjustment of contrast usually gets it right for
the majority of negatives. Only in cases where the neg is very bright,
dark, low contrast, or very high contrast, does it have problems with
auto adjustment.

I'm just a happy user. No connection with Epson. Perhaps I should have!

I think the only thing that would be really cool would be an automatic
negative changer for when I am scanning 30 negs of industrial contract
work, where the contrast is almost the same for each one! Anyway, I can
dream.

Best wishes

Ian

} } Does anyone have experience of Epson scanners for TEM negatives? I have
} } enquired of the company whether the Epson Perfection 2450 Photo can take
}
} our
}
} } 4 x 3-and-a quarter inch TEM plates,
} }
} } but all I can get is the standard answer "we have film holders", which
} } include 120 roll film and 5 x 4 inch, but not the size we want. Can one
} } simply place the negative straight down on the scanner surface?
} }
} } Any help would be much appreciated,
} }
} } (Sorry if you've received this message before - I've had one or two failed
} } attempts at sending).
} }
} } -----------------------------------
} } Robert H. Olley, Physics Dept,
} } Univ. Reading, RG6 6AF, England.
} } E-mail: R.H.Olley-at-reading.ac.uk
} } URL: http://www.rdg.ac.uk/~spsolley
} } -----------------------------------

--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Thu Nov 7 07:03:39 2002



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Thu, 07 Nov 2002 08:56:10 -0300 (ADT)
Subject: re:TEM polycontrast

Contents Retrieved from Microscopy Listserver Archives
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Morning Sharon,

A typical turn -around time is 2-3 days, with four days being the latest
that samples should be done by. These numbers come from many years of on
the job numbers.
Does your boss not help out at all in the lab?
How many chucks for the microtome do you have? You should have at least 5,
so you are not wasting time changing blocks all the time. Tissue processor?
Film and paper processors?

On average I was able to complete 2 (possibly 3) cases/day. Of course these
are averages. Some times the do-do does hit the fan.

Ed Calomeni
currently unemployed EM tech
----- Original Message -----
} From: "Sharon Drew (by way of MicroscopyListserver)" {drewsh-at-musc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, November 06, 2002 5:26 PM


Hi Leslie!
We have been using polycontrast paper for a long time (more likely
always). I find it much easier to handle then graded paper. We
change filters to adjust contrats and play with exposure time. I think
it is more economical way of printing then using graded paper.
Dorota


From daemon Thu Nov 7 08:47:57 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 07 Nov 2002 09:39:34 -0500
Subject: Re: B & W film

Contents Retrieved from Microscopy Listserver Archives
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I think LPD can still be purchased from Freestyle Sales in southern
California.

Geoff

Paula Sicurello wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} ** Reply Requested When Convenient **
}
} Hi listers,
}
} I'm looking for a black and white reversal film. I have some old LPD4
} but I can't kind listed anywhere (fuji or kodak) if they still make this
} film. And if they do make it, does it come in little rolls or for a
} bulk loader?
}
} I'm lazy and I like to make B&W text slides with B&W reversal film. Or
} can I use a color slide film and still have it turn out OK?
}
} Any information is gladly accepted and I thank you in advance.
}
} Paula :-)
}
} p.s. some of us are old fashioned and like to use film instead of all
} the fancy-schmancy computer thingies ;)
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Nov 7 09:09:17 2002



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Thu, 07 Nov 2002 09:06:25 -0800
Subject: Re: vibration?

Contents Retrieved from Microscopy Listserver Archives
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The suggestion of discriminating between AC fields and vibration via working
distance is a good one. Another useful test is to change the beam voltage -- the
susceptibility to transverse magnetic fields increases as the beam voltage is
lowered. The (approximate) equation goes like this:
Deflection = (0.5 microns)* B * Z^2 / sqrt( V )
Where B is the transverse field in gauss, Z is the working distance in mm, and V is
the beam voltage in kilovolts. So the variation of deflection (sawtooth height)
with working distance is stronger, but it isn't always practical to make a large
change in WD, whereas it shouldn't be hard to get a factor of two variation by the
beam voltage method (e.g., 20 kev and 5 kev). I tend to prefer the beam voltage
method because the stage is a complex mechanical device whose suspectibility to
vibration may itself be a function of height, thereby sometimes confusing the
results.

Fred Schamber
ASPEX

Benjamin - Simkin wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Ann, I agree with Allen Sampson's comments, with the addition that you could
} also get the same effect from AC magnetic fields. These can pop up from a
} number of sources (computer equipment, electroc motors, overhead lights, the
} list goes on...).
} A way of checking if it's vibration as opposed to fields is to check the
} magnitude of the 'sawteeth' at long and short working distances; if it's fields,
} then the sawteeth will be bigger at longer working distances (because the action
} of the field will be operating over a longer distance at the longer working
} distance). This also serves as a crude short-term fix for the problem sometimes,
} although I'd really recommend finding the piece of equipment that's causing
} the problem, and fix that instead.
} Another possible source is a ground loop, where your 'scope is hooked up to
} another piece of equipment with a differant ground potential. This causes
} currents to end up flowing through your equipment-'scope connection, generating
} the AC fields internally. A solution for this is to decide on a single
} ground potential to use (usually the 'scope's dedicated ground), and then
} hardwire all the other equipment to this one ground. (The best case, of course,
} is to sink your own ground, but many people/labs don't have this option
} available.)
} If you do have field problems, you can usually track them down using an
} AC magnetic field hand meter, which you can get for around $35 from an
} online dealer (I forget where we got ours now, sorry).
}
} I hope this helps,
}
} Ben Simkin (simkin-at-egr.msu.edu)
} Michigan State University
} Department of Chemical Engineering and Materials Science



From daemon Thu Nov 7 09:23:10 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Thu, 07 Nov 2002 15:54:08 +0100
Subject: Re: 16-bit images

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As far as I knew, the 16 bit support is in newer versions of Photoshop,
but I haven't used it much. It's certainly in version 6, which I use.
Perhaps it still has limited functionality. Worth testing, perhaps.

Ian

Thor Bostrom schrieb:
} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Robert and listers,
}
} We have a recently acquired Epson Perfection 2450 Photo scanner and it
} works very well with TEM negs mounted in the 5 x 4 film holder. You want
} to avoid placing the negs directly on the glass surface because of Moire
} fringes. (Curiously we had little trouble with fringes on our old HP
} transparency scanner.)
}
} The Epson scanner can acquire 16-bit grayscale TIFF images which can be
} advantageous for negatives with very low contrast. However few software
} packages seem to handle this format. DigitalMicrograph and analySIS do,
} while Adobe Photoshop provides some limited support. Are there other
} programs out there that can work with this format?
}
} With thanks,
} Thor
}
} } = = = = = = = = = = = = = = = = = = =
} }
} } Does anyone have experience of Epson scanners for TEM negatives? I have
} } enquired of the company whether the Epson Perfection 2450 Photo can take
} } our 4 x 3-and-a quarter inch TEM plates,
} } but all I can get is the standard answer "we have film holders", which
} } include 120 roll film and 5 x 4 inch, but not the size we want. Can one
} } simply place the negative straight down on the scanner surface?
} }
} } Any help would be much appreciated,
} }
} } (Sorry if you've received this message before - I've had one or two
} failed
} } attempts at sending).
} } -----------------------------------
} } Robert H. Olley, Physics Dept,
} } Univ. Reading, RG6 6AF, England.
} } E-mail: R.H.Olley-at-reading.ac.uk
} } URL: http://www.rdg.ac.uk/~spsolley
} } -----------------------------------
} }
} =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
} Dr Thor Bostrom
} Acting Director,
} Analytical EM Facility,
} Faculty of Science,
} Queensland University of Technology (QUT)
} GPO Box 2434, Brisbane, QLD 4001, Australia
} Ph: +61 7 3864-2351 FAX: +61 7 3864-5100
} http://www.sci.qut.edu.au/aemf/
} =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
}
}
}


--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Thu Nov 7 09:24:52 2002



From: gary.m.brown-at-exxonmobil.com
Date: Thu, 7 Nov 2002 09:18:11 -0600
Subject: Re: ZAF numbers and K ratios

Contents Retrieved from Microscopy Listserver Archives
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Anthony,

NIST (as I recall) developed the Desk Top Spectrum Analyzer (DTSA) that
will import spectra and allow one to run quantitative analysis routines. An
e-mail to Dale Newbury (see cc: list) at NIST may help.

Good luck,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



"Anthony Rinaldi"
{Anthony_Rinaldi-at-J To: "'Microscopy-at-MSA.Microscopy.Com'"
abil.com} {Microscopy-at-sparc5.microscopy.com}
cc:
Subject: ZAF numbers and K ratios
11/06/02 09:59 PM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am using a rather old (almost antique) EDS system for my X ray mapping.
It
is so old that it will not do quantitative analysis. With the economy like
it is I will have to make this equipment last a few more years.

Does anyone know of a program that I can use that contains the reference
data tables and allows you to compute the ZAF number (or K ratios ) for
different elements? Doing this by hand is quite tedious. Thank you.

Anthony Rinaldi








From daemon Thu Nov 7 09:33:43 2002



From: Anthony Rinaldi :      Anthony_Rinaldi-at-Jabil.com
Date: Thu, 7 Nov 2002 10:26:34 -0500
Subject: Re: ZAF numbers and K ratios

Contents Retrieved from Microscopy Listserver Archives
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Gary,

Thank you for the response. I did find that program. It looks like it
imports the data file and then analyzes it. I do not believe that my EDS
system has the ability to export the data. I have to take a picture of the
screen. It has no disk option to transfer a file.

Since I can't transfer the file, I was hoping for something that would help
me with the math part. I did get a program from Mr. Kaker which shows
promise. However, I will need to learn about what each factor is in order to
use it. If you have any recommendations for course or books please let me
know.

Again thank you for your response.

Anthony Rinaldi
Lead Failure Analysis Engineer
Jabil Circuit, St Petersburg FL
727-803-3695

-----Original Message-----
} From: gary.m.brown-at-exxonmobil.com [mailto:gary.m.brown-at-exxonmobil.com]
Sent: Thursday, November 07, 2002 10:18 AM
To: Anthony_Rinaldi-at-Jabil.com; microscopy-at-sparc5.microscopy.com
Cc: newbury-at-enh.nist.gov


Hello,

I am using a rather old (almost antique) EDS system for my X ray mapping.
It
is so old that it will not do quantitative analysis. With the economy like
it is I will have to make this equipment last a few more years.

Does anyone know of a program that I can use that contains the reference
data tables and allows you to compute the ZAF number (or K ratios ) for
different elements? Doing this by hand is quite tedious. Thank you.

Anthony Rinaldi









From daemon Thu Nov 7 09:34:21 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Thu, 7 Nov 2002 10:27:48 -0500
Subject: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
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Dear Leslie:

We used to use the polycontrast paper before we switched to the
kodabromide II RC paper. Also, since we are in Rochester, NY the home of
Eastman Kodak I can tell you that Kodak still produces Kodabromide in grades
2, 3, & 4.

They have also introduced a Polymax II RC paper which has a set of
filters which allow for even greater range of contrasts. I would recommend
you change to the Polymax II RC paper. For instance using filter 4 gives
you equivalent contrast to the Kodabromide grade 4 paper, filter 5 the grade
5 paper. The lower filters give you grades 1-3 equivalents.

Try the Polymax, you'll like it.....

Karen Bentley

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954


-----Original Message-----
} From: Leslie Cummins [mailto:gunther-at-aecom.yu.edu]
Sent: Wednesday, November 06, 2002 3:06 PM
To: Microscopy-at-sparc5.microscopy.com


Hello listers

As Kodak slowly discontinues the graded Kodabrome II RC papers (no 1, 4 or
5), we are looking for alternatives for our printing.

We have a Durst point source enlarger, and I was wondering if we can switch
to Polycontrast paper and filters. If there are any labs that are using
this method, I would appreciate any information on how well it works.

We have found that it is still more economical and time efficient to
develop and print 8 x 10 study prints for our users, rather than scan in
all our negatives, so any old-fashioned wet darkroom suggestions would be
appreciated.

Thanks in advance
Leslie


Leslie Gunther Cummins
Analytical Imaging Facility
Albert Einstein College of Medicine
1300 Morris Park Ave.
Bronx, NY 10461
718-430-3547

http://www.aecom.yu.edu/aif/



From daemon Thu Nov 7 10:21:55 2002



From: Lenaldo Branco Rocha :      lenaldo-at-rpa.fmrp.usp.br
Date: Thu, 07 Nov 2002 13:42:18 -0200
Subject: LM: help using Osteobed and Histocryl resins

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Dear friends

I've been trying to using Osteobed resin to embed small calcified bone
samples. However I've got problems with the polymerization step, the resin
becomes a rubber-like mass. I tried the manufacturer's protocol and few
variations for methylmetacrilate found in literature but none of them
worked. Any hint?

And regarding Histocryl, has anyone tried any immnuhistochemical or
histoenzymology protocol in tissues embedded in it? Does it need the removal
of resin prior the reaction?

I would be grateful with any help.

Best regards,
Lenaldo




--
Lenaldo Branco Rocha, DDS, MSc
Faculty of Medicine of Ribeirão Preto - USP
Department of Pathology
Av. Bandeirantes, 3900
Monte Alegre
Ribeirão Preto - SP - Brazil
14049-900
---------------------------------
Tel: +55-16-602-3132
lenaldo-at-rpa.fmrp.usp.br



From daemon Thu Nov 7 10:40:49 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Thu, 7 Nov 2002 11:34:02 -0500
Subject: RE: Staining Spurr's Resin

Contents Retrieved from Microscopy Listserver Archives
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(I've tried to post this but it was bounced - and in light of the recent
emails about the hotmail I'm trying to send this as plain text)

I sent out an earlier reply to the first post:

I've had zero problems with contrast with Spurr's if the sections are
thick enough (gold post heat stretching).

I've used the xylene to stretch them on the boat, but found the heat
'pen' that we got from EMS works just as well without the risk to all
the sections in the boat and without the vapors.

Sections are cut so that when they are stretched they are gold.

To pick them up a quick pass through the alcohol flame is good enough to
get them to stick, with a trip into the 55 C oven until they reach temp
to keep them on the grid.

Staining is simple

20-30 min submerged in a porcelain well filled with a saturated aqueous
UA solution (use carpet tape to keep the UA bottle stable and keep a
sediment of crystal UA on the bottom - keep the bottle covered and
labeled). 2-5 minutes in the calcined lead citrate solution in a CO2
free environment (coverslip with NaOH pellet lining the walls)

quick coat of carbon in the evaporator - and - well I've got new batches
of students each spring that don't seem to have any problems staining
Spurr's with the above method.

But of Course (car talk) YMMV (your mileage may vary) but to adapt it:
YRMV (results)


Geoff Williams
Microscopy Facility Supervisor
 
Checkout the new Biology Department Microscopy Facility web page. 
Version 1 is now On-Line:
www.cst.cmich.edu/users/willi1gl/BDMF/BDMF.htm




From daemon Thu Nov 7 10:51:30 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 7 Nov 2002 11:44:28 -0500
Subject: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I haven't been in a darkroom in a long time, but I did spend many hours in one. I printed with both the Kodabrome II RC and the Polycontrast II RC and very good results with both. You will have to figure out the correlation between the grade for the Kodabrome and the contrast filter to use with the Polycontrast papers, but there are Kodak darkroom guides that you can use. They have images printed on both the Polycontrast and Kodabrome with the different filters and grades. You can use them to give you the equivalent values for what you wanted to do. Once you know what they are, you use the same filters for your images. For example, a soft filter for diffraction patterns and a hard filter for low contrast images such as HRTEM.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Leslie Cummins [mailto:gunther-at-aecom.yu.edu]
Sent: Wednesday, November 06, 2002 3:06 PM
To: Microscopy-at-sparc5.microscopy.com


Hello listers

As Kodak slowly discontinues the graded Kodabrome II RC papers (no 1, 4 or
5), we are looking for alternatives for our printing.

We have a Durst point source enlarger, and I was wondering if we can switch
to Polycontrast paper and filters. If there are any labs that are using
this method, I would appreciate any information on how well it works.

We have found that it is still more economical and time efficient to
develop and print 8 x 10 study prints for our users, rather than scan in
all our negatives, so any old-fashioned wet darkroom suggestions would be
appreciated.

Thanks in advance
Leslie


Leslie Gunther Cummins
Analytical Imaging Facility
Albert Einstein College of Medicine
1300 Morris Park Ave.
Bronx, NY 10461
718-430-3547

http://www.aecom.yu.edu/aif/



From daemon Thu Nov 7 11:05:00 2002



From: Eric :      biology-at-ucla.edu
Date: Thu, 7 Nov 2002 08:57:16 -0800
Subject: Re: Clinical turn around

Contents Retrieved from Microscopy Listserver Archives
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Hiya Sharon,

Well, for us turnaround is 3 days because we have a digital camera on our
microscope. Eliminates darkroom time...
There are two of us in the lab. Sometimes I personally can have 3-4 cases a
day cut and scoped. On a good day.

Here we get anywhere from 1-12 cases a day in the lab.

============================================================

} } Hi!
} } I run a diagnostic TEM laboratory in SC.
} } One tech.
} } 6 to 5 samples every other day at least.
} } What is the turn around time asked for your labs out there that are
} } also doing diagnostic/clinical work and where does that number come
} } from?
} } Thank you for any help.
} } My supervisor is saying 2 days but I think that is close to impossible
} } when only one tech doing everything including transport of tissue from
} } another lab and scoping all case that come in.
} } Sharon Drew
} } Diagnostic Pathology EM
} } Charleston, SC
} }
}
}



From daemon Thu Nov 7 11:32:45 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Thu, 07 Nov 2002 09:24:10 -0800
Subject: Re: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I always used polycontrast paper and filters on a Durst point source
enlarger, until everything went digital. I found that I had better control
that way, as there is a wider range of filters than papers. It meant a
little more work at the beginning, since test strips had to be done one at a
time, but the results were worth it.

Lesley Weston.



on 06/11/2002 12:05 PM, Leslie Cummins at gunther-at-aecom.yu.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello listers
}
} As Kodak slowly discontinues the graded Kodabrome II RC papers (no 1, 4 or
} 5), we are looking for alternatives for our printing.
}
} We have a Durst point source enlarger, and I was wondering if we can switch
} to Polycontrast paper and filters. If there are any labs that are using
} this method, I would appreciate any information on how well it works.
}
} We have found that it is still more economical and time efficient to
} develop and print 8 x 10 study prints for our users, rather than scan in
} all our negatives, so any old-fashioned wet darkroom suggestions would be
} appreciated.
}
} Thanks in advance
} Leslie
}
}
} Leslie Gunther Cummins
} Analytical Imaging Facility
} Albert Einstein College of Medicine
} 1300 Morris Park Ave.
} Bronx, NY 10461
} 718-430-3547
}
} http://www.aecom.yu.edu/aif/
}
}



From daemon Thu Nov 7 13:03:23 2002



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 7 Nov 2002 12:50:04 -0800
Subject: RE: vibration?

Contents Retrieved from Microscopy Listserver Archives
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Ben,

} From the pictures she posted, the problem is not AC electro-magnetic
fields. They occur at 50 or 60 Hertz. Unless she has an SEM capable of
incredible secondary electron collection, the images she posted were taken
with an exposure time of at least 60 seconds (assuming a 4000 line
exposure, 30 sec for 2000 line, 15 seconds for 1000, etc.). At this rate
the vibrations shown are much slower than 60 Hz. Just divide the number of
saw teeth seen by the exposure time.

For your information, vibrations will also increase with working distance.
Within the electron optics column, where the beam is being acted on by the
condenser lens and other magnetic lenses, the electron beam is pretty much
constrained to the beamline by the action of the lenses - any external
motion or magnetic field that attempts to moves the beam will be offset by
the lens's symmetrical action to contain the beam. Within the sample
chamber, the beam's path has been pre-determined by the actions of the c
olumn and is influenced only by the unexpected, and external, influences.

Thanks, however, for mentioning the effect of working distance - I forgot
to mention it before. With both vibrations and electro-magnetic
interference, the majority of the influence on the beam will be in the
portion of time that the beam spends in traveling from the final pole piece
to the sample (the working distance). Decreasing the working distance
will, in all cases, decrease the affect.

As far as ground loops - this is an area that is generally not very well
understood. I have never really seen an instrument installation that
suffered from this problem, at least as it is generally understood. The
electrical ground is actually not something that generally has an active
role in electrical systems. From the wall into your system, you have
electrical connections to hot and neutral as well as an electrical ground.
The hot and neutral wires are connected to the phase and center tap of the
nearest step down transformer respectively. The ground is connected to a
local source of ground potential.

Local ground potentials can vary considerably and this connection is
primarily used as a safety to ensure that anything contacting a grounded
connection will not be at a dangerous voltage level from anything else
(such as water lines) that are at a local ground level. The neutral
electrical connection will be grounded by the power company, but not
necessarily at a point local to the delivery of power.

This can be best explained, perhaps, by the major problem with lightning
strikes. In most cases of lightning strikes, the actual power delivery
lines are not hit. Instead, the strike hits a tree or the ground nearby,
which increases the local ground potential and causes breakdowns between
components and ground. The electrical ground is normally only used to
protect people by connecting the exterior surfaces of systems to a local
potential that won't result in harm to people should a large incursion in
local ground potential happen.

Equipment within a building will generally not connect anything to
electrical ground other than the external cases. Should anything within
the system short to a surface anyone might touch, this assures that the
only voltages encountered will be at the local ground potential.

Having said that, I have to point out the poor nomenclature we have chosen
in this regard. The ground loops that can cause problems in instruments
this sensitive, are actually those on the electrical 'grounds' of the power
supplies. The term 'ground', in this case, is normally applied to the
common connection when we use multiple power supplies. For example, if we
have a system that has both a +5 volt and a +15 volt power supply, we often
tie the negative connection from each power supply together into a common
'ground'. If one of those supplies, or the circuits it connects to, tends
to pull its negative voltage higher, we have a ground loop as it will also
affect the voltage on the other supply. Since both sides of power supplies
will often be completely isolated from the incoming power by transformers
within the instrument, this 'common' ground may have no relationship
whatsoever to any real ground voltage. I know this sounds confusing, but
this is the true meaning of ground loop.

In other words, the external connection of individual equipment cases to
each other does little good as they are generally already each connected to
local ground through the wall connections. The real trick is to connect
the power supply common connections in such a way as to provide as low a
resistance path as possible to the chosen 'common' power connection.



Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, November 06, 2002 5:37 PM, Benjamin - Simkin
[SMTP:simkin-at-egr.msu.edu] wrote:
}
} Ann, I agree with Allen Sampson's comments, with the addition that you
could
} also get the same effect from AC magnetic fields. These can pop up from a
} number of sources (computer equipment, electroc motors, overhead lights,
the
} list goes on...).
} A way of checking if it's vibration as opposed to fields is to check
the
} magnitude of the 'sawteeth' at long and short working distances; if it's
fields,
} then the sawteeth will be bigger at longer working distances (because the
action
} of the field will be operating over a longer distance at the longer
working
} distance). This also serves as a crude short-term fix for the problem
sometimes,
} although I'd really recommend finding the piece of equipment that's
causing
} the problem, and fix that instead.
} Another possible source is a ground loop, where your 'scope is hooked
up to
} another piece of equipment with a differant ground potential. This causes
} currents to end up flowing through your equipment-'scope connection,
generating
} the AC fields internally. A solution for this is to decide on a single
} ground potential to use (usually the 'scope's dedicated ground), and then
} hardwire all the other equipment to this one ground. (The best case, of
course,
} is to sink your own ground, but many people/labs don't have this option
} available.)
} If you do have field problems, you can usually track them down using
an
} AC magnetic field hand meter, which you can get for around $35 from an
} online dealer (I forget where we got ours now, sorry).
}
} I hope this helps,
}
} Ben Simkin (simkin-at-egr.msu.edu)
} Michigan State University
} Department of Chemical Engineering and Materials Science
}
}
}
}



From daemon Thu Nov 7 13:03:24 2002



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 7 Nov 2002 12:50:20 -0800
Subject: RE: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Vitaly,

Most of your concerns I covered in a previous reply. However, I have to
take exception to your claim that a large change in the problem's
characteristic would result from a change in accelerating voltage only if
the problem was electro-magnetic. A reduction in the accelerating voltage
will also result in a reduction of the velocity of electrons in the beam.
That would cause an increased displacement of the beam as it traverses the
sample surface due to vibrations.

In other words, the effects of electro-magnetic interference and vibrations
are virtually inseparable except for the frequency involved. Each will
affect the beam in similar ways, in regards to accelerating voltage and
working distance. Both will have a greater affect with a lower
accelerating voltage as well as a greater working distance.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, November 06, 2002 8:58 PM, Vitaly Feingold
[SMTP:vitalylazar-at-worldnet.att.net] wrote:
} Could be vibration. Could also be magnetic field. And, could be signal
} interference, such as ground loop. What type of SEM are you using?
}
} I will omit remedies for the vibration problem, since Allen Sampson did
} pretty good job addressing that.
}
} Stray AC line frequency magnetic field: try acquiring image at different
} accelerating voltages. It is very important that all other geometry
} parameters will remain the same: particular area of a particular
specimen,
} WD, tilt, magnification, spot size. Contrast, brightness, focus, and
} stigmator settings can be changed. If the problem is much worse at, say,
2
} kV, than at, say, 15kV, you are dealing with magnetic field. You may also
} try to keep 3 axes AC milliGauss meter at the SEM, and notice what it
} measures, but catching stray magnetic interference this way may be a
little
} tricky. These meters, though, are very inexpensive, and available from
many
} test instruments suppliers.
}
} Now we are getting down to statistically most likely problem- signal
} interference and ground loops. It is difficult to go any further without
} knowing the SEM type, and acquisition system (if separate) type. Too many
} possibilities exist, but the very first- does your acquisition software
} have an option somewhere in the settings which reads something like "60
} Hertz sync", or "AC line synchronization", or "Line sync", etc., etc.? If
} yes, check (or uncheck) that option and see what happens. Further
remedies
} of this problem will include eliminating ground loops. Example- shielded
} cable(s) has shield connected on both sides, and shield is used as one of
} the signal wires. This is frequently done, and does jeopardize instrument
} interference tolerance. Another example- one of the accessories is
} susceptible to some kind of interference, and must be powered through the
} isolation transformer. The latter remedy will only work with decent
(better
} if dedicated) ground. The fact that the problem is not present all the
time
} does not mean that everything is perfect inside the SEM. Besides, going
} after the EM interference source could be inefficient and frustrating, if
} not impossible. Improving grounding/shielding/power connection might be
} easier.
}
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
}
} This address can not receive messages larger than 15 kb without prior
} notification.
}
} ----- Original Message -----
} } From: Ann-Fook Yang {yanga-at-agr.gc.ca}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, November 06, 2002 2:45 PM
} Subject: vibration?
}
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } Hi everyone,
} }
} } I have two SEM micrographs on the web:
} } http://www.magma.ca/~scimat/Defect.htm
} } The micrographs show zagged edges taken at 20 kX. Such phenomenon does
} not present all the time. Can anyone suggest what causes the problem and
how
} to solve it?
} } Thanking in advance.
} }
} }
} }
} }
} }
} }
} }
} }
} }
} } AnnFook Yang
} } EM Unit,
} } Eastern Cereal and Oilseed Research Centre,
} } Room 2091, Bldg. 20,
} } Central Experimental Farm,
} } Ottawa, Ontario
} } Canada K1A 0C6
} }
} } Tel: 1-613-759-1638
} } Fax: 1-613-759-1701
} }
} } e-mail: yanga-at-em.agr.ca
} }
} }
} }
}
}
}
}
}



From daemon Thu Nov 7 13:06:11 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 7 Nov 2002 13:58:34 -0500
Subject: B & W film

Contents Retrieved from Microscopy Listserver Archives
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Paula,
I have an old (1973) Kodak publication entitled, "Small-Batch
Reversal Processing of KODAK B/W Films" , #J-1. Not only use of Reversal
kits but also make from scratch formulations.
It is on its way to you at the FAX number you have listed below.
Let me know if it doesn't come thru alright.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone: 610-738-0437
eMail: fmonson-at-wcupa.edu


-----Original Message-----
} From: Paula Sicurello [mailto:patpxs-at-gwumc.edu]
Sent: Wednesday, November 06, 2002 2:56 PM
To: microscopy-at-sparc5.microscopy.com


** Reply Requested When Convenient **

Hi listers,

I'm looking for a black and white reversal film. I have some old LPD4
but I can't kind listed anywhere (fuji or kodak) if they still make this
film. And if they do make it, does it come in little rolls or for a
bulk loader?

I'm lazy and I like to make B&W text slides with B&W reversal film. Or
can I use a color slide film and still have it turn out OK?

Any information is gladly accepted and I thank you in advance.

Paula :-)

p.s. some of us are old fashioned and like to use film instead of all
the fancy-schmancy computer thingies ;)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax


From daemon Thu Nov 7 13:25:31 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 7 Nov 2002 19:06:51 -0000
Subject: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



This artefact may be due to vibration, but I don't think it is
possible to confirm that without additional information. The waves
seem suspiciously regular.
Acoustic vibration is rarely at a single frequency, and the pitch of
the acoustically-induced
sawtooth is usually rather variable. Also, acoustically-induced
vibration will improve when the stage is
locked or clamped (if you have that facility). I would also consider
the possibility that there is an electronic
problem in the scan generator circuit. On both of my last two sems
(different manufacturers) similar faults
to those shown were caused by faulty transistors. Other possible
causes are electrical interference carried by the
electricity supply, or strong electromagnetic fields generated by
e.g. nearby electric
motors, power transformers, etc. As noted by Benjamin, interference
caused by mag field changes
with working distance, but so does interference from acoustic
vibration, usually appearing
worse at long working distance.
Chris

} } } Hi everyone,
} } }
} } } I have two SEM micrographs on the web:
} } } http://www.magma.ca/~scimat/Defect.htm
} } } The micrographs show zagged edges taken at 20 kX. Such
phenomenon
} } does not present all the time. Can anyone suggest what causes the
} } problem and how to solve it?
} } } Thanking in advance.
} } }
} } } AnnFook Yang
} } } EM Unit,
} } } Eastern Cereal and Oilseed Research Centre,
} } } Room 2091, Bldg. 20,
} } } Central Experimental Farm,
} } } Ottawa, Ontario
} } } Canada K1A 0C6
} } }
} } } Tel: 1-613-759-1638
} } } Fax: 1-613-759-1701
} } }
} } } e-mail: yanga-at-em.agr.ca
} } }
} } }
} }
}



From daemon Thu Nov 7 14:34:36 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 07 Nov 2002 12:32:56 -0800
Subject: Re: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looks like a failing turbo pump to me. Does this
SEM have turbo?

gary g.


At 11:45 AM 11/6/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Nov 7 15:19:27 2002



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Fri, 08 Nov 2002 08:08:41 +1100
Subject: Re: Fw: ....16-bit images

Contents Retrieved from Microscopy Listserver Archives
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Hi Thor,
ImageJ (the free, multiplatform Java incarnation of NIH
Image) will handle up to 32bit grey images.
Website http://rsb.info.nih.gov/ij/
cheers

Sally.


} } } Thor Bostrom {t.bostrom-at-qut.edu.au} 11/07/02 01:46PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Robert and listers,

We have a recently acquired Epson Perfection 2450 Photo scanner and it
works very well with TEM negs mounted in the 5 x 4 film holder. You want to

avoid placing the negs directly on the glass surface because of Moire
fringes. (Curiously we had little trouble with fringes on our old HP
transparency scanner.)

The Epson scanner can acquire 16-bit grayscale TIFF images which can be
advantageous for negatives with very low contrast. However few software
packages seem to handle this format. DigitalMicrograph and analySIS do,
while Adobe Photoshop provides some limited support. Are there other
programs out there that can work with this format?

With thanks,
Thor

} = = = = = = = = = = = = = = = = = = =
}
} Does anyone have experience of Epson scanners for TEM negatives? I have
} enquired of the company whether the Epson Perfection 2450 Photo can
take
} our 4 x 3-and-a quarter inch TEM plates,
} but all I can get is the standard answer "we have film holders", which
} include 120 roll film and 5 x 4 inch, but not the size we want. Can one
} simply place the negative straight down on the scanner surface?
}
} Any help would be much appreciated,
}
} (Sorry if you've received this message before - I've had one or two
failed
} attempts at sending).
} -----------------------------------
} Robert H. Olley, Physics Dept,
} Univ. Reading, RG6 6AF, England.
} E-mail: R.H.Olley-at-reading.ac.uk
} URL: http://www.rdg.ac.uk/~spsolley
} -----------------------------------
}
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Dr Thor Bostrom
Acting Director,
Analytical EM Facility,
Faculty of Science,
Queensland University of Technology (QUT)
GPO Box 2434, Brisbane, QLD 4001, Australia
Ph: +61 7 3864-2351 FAX: +61 7 3864-5100
http://www.sci.qut.edu.au/aemf/
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=




From daemon Thu Nov 7 15:36:35 2002



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Thu, 7 Nov 2002 16:29:05 -0500
Subject: Kodak Rapid Fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Why is it that the directions from Kodak for making a gallon of Rapid
Fixer state that I should pour 32oz. of "A" and 104ml of "B" into a
half gallon of water and then make it up to 1 gal. but the kit to
make 1 Gal. has 33oz of "A" and 106ml of "B"?

Also for people who now seldom need to use fixer - be advised that
"A" DOES go bad with age. You will know it when a precipitate forms
as "B" is added slowly to the mixture of water and "A". If the "A" is
really old there will be the signs of yellow crystals on the sides of
the container.

I questioned Kodak representatives in the mid-1980's and again in the
1990's and was told not to worry. I still worry!

Pat Connelly
Univ. of Pennsylvania
Biology Dept.



From daemon Thu Nov 7 15:48:47 2002



From: sghoshro-at-NMSU.Edu
Date: Thu, 7 Nov 2002 14:41:15 -0700 (MST)
Subject: Re: Plant/Botanical EM books

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,

Try the following books,

Methods in Plant Electron Microscopy and Cytochemistry
by William V. Dashek (Editor)

Plant Cell Biology: Structure and Function
by Brian E. S. Gunning, Martin W. Steer

I routinely work with plant tissue. if you need any help in the future,
just contact me, we can send you protocols.

Good luck.

Soumitra

*************************************************************
Soumitra Ghoshroy Ph.D.
College Associate Professor
Department of Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml

On Wed, 6 Nov 2002, Jensen, Karen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Dear Listers:
} }
} } I have recently had several requests to process and embedd plant
} } tissue. All of my previous EM experience has involved only human and
} } animal tissue.
} }
} } Those of you who routinely do botanical specimens can you recommend
} } some EM books or atlases which cover processing methods?
} }
} } Are there any atlases that provide ultrastructural images so I can
} } learn the morphology/terminology of different types of plant specimens.
} } Right now I will be looking at potato leaves and tubers. Later I am
} } supposed to get a tomato for EM processing.
} }
} } Thanks for your help!
} }
} } Karen L. Bentley, M.S.(previously Jensen)
} } Associate Scientist & Project Manager
} } Electron Microscope Research Core
} } University of Rochester Medical Center
} } Rochester, NY 14642
} } 585-275-1954
} }
}
}



From daemon Thu Nov 7 16:55:15 2002



From: John T. Armstrong :      john.armstrong-at-nist.gov
Date: Thu, 07 Nov 2002 17:48:41 -0500
Subject: Re: ZAF numbers and K ratios

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anthony,

In a separate e-mail, I will be attaching a set of files containing the
NIST "CITZAF" stand-alone ZAF analysis package. The files, once extracted
contain two PDF files describing the operation of the various programs as
well as the CITZAF program package. This program has been around for some
time in various forms. The attached files contain the current (beta-test)
development version of this program package that we are using at NIST. We
are currently modifying the input procedures of this program and
"window-fying" it. Once completed, the updated program will be available
on the NIST Chemical Science and Technology Laboratory, Surface and
Microanalysis Division web page -- www . cstl . nist . gov / div837 /
Division . I hope you will find this useful.

Best regards,

John Armstrong



At 10:59 PM 11/6/2002 -0500, Anthony Rinaldi wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*******************************
Dr. John T. Armstrong
Nat'l Inst. of Standards & Tech.
Chemistry, Bldg. 222, Rm A113
100 Bureau Drive. Stop 8371
Gaithersburg, MD 20879-8371
(301) 975-3929
(301) 417-1321 (FAX)
john.armstrong-at-nist.gov



From daemon Thu Nov 7 18:21:41 2002



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Thu, 07 Nov 2002 18:09:21 -0600
Subject: Re: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Leslie,
I use a Durst Laborator S-45 Special enlarger with a point light source
and print all my TEM and 35mm negatives on Ilford Multigrade IV RC DeLuxe
paper using Ilford Multigrade filters. It works fine for me and my bosses,
but I am sure that other papers like Kodak Polymax II RC, as suggested by
Karen Jensen, work just as well.
I learned my darkroom work using fiber-based graded papers and drying them
on a big mirrored drum, but now must I air dry my prints ever since our
dryer broke and couldn't be fixed. This is a bit of a nuisance as the
prints take overnight to dry and can't be turned in the same day they're
printed.
Does anyone have tips on drying resin coated paper quickly???
Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242-1324



From daemon Fri Nov 8 00:37:40 2002



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Fri, 08 Nov 2002 07:29:53 +0100
Subject: Re: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
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Hi

SORRY - I forgot to give the author - it is by Rhodin
Histology: A text and atlas takes a lot of beating - it goes from LM
through to EM. It is out of print now but Amazon.com still has some copies
available.


At 09:14 2002-11-06 -0500, Julian Smith III wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Obs/NB New postal/visiting address from July 2002!

Med vänliga hälsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 5858 1038
Fax +46 728 3747

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Huddinge Universitetssjukhus, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.



From daemon Fri Nov 8 01:02:40 2002



From: Marion Stevens-Kalceff :      Marion.Stevens-Kalceff-at-unsw.edu.au
Date: Fri, 08 Nov 2002 17:57:17 +1100
Subject: SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey Allen,

You may found the following figures entertaining. Natural
constants and results of calculations are rounded to the first character
after the decimal point, relativity effects are neglected, plain text format
is used. I am a bit rusty on my Physics- my apologies if I missed an order
or so of magnitude. Makes no difference in this example.

Electron mass ~ 9.1 x 10-31kg
Electron charge ~ -1.6 x 10-19 C
One electronvolt energy ~ 1.6 x 10-19J
Kinetic energy (of electron, or anything having mass) = mV2/2, where m is
the mass,
V2 is the velocity square.

Simple calculations yield the following velocities in kilometers per second
for electrons accelerated by potential differences of:
1 Volt ~ 6 x 100 km/s
200 V ~ 8.4 x 1,000 km/s
2 kV ~ 2,7 x 10,000 km/s
15 kV ~ 7.3 x 10,000 km/s

Amazing, isn't it?

Mechanical vibration velocity must be compatible in magnitude with the above
velocities, in order for vibration effects to look different at different
beam accelerating voltages. But SEM is made of the materials found on this
planet, with limited durability specifications. Thus, in order to vibrate
with such velocities, SEM must be either made very tiny, which is not the
case, or must disintegrate into dust, but it doesn't.

Velocities of mechanical vibrations affecting SEM are in many orders of
magnitude slower
than the E-beam velocities.

This is why SEM stage vibration will show up exactly the same at different
beam accelerating voltages.

Why then stray magnetic field affects an e-beam? Because electron has huge
charge/mass ratio, ~ 10,000,000,000. The interaction is very strong. Thus,
effect increases at lower accelerating voltages. Same with working distance-
effect increases with the distance increase. But, one needs to change WD
substantially, to see the effect for sure. That will reduce the resolution,
among other things. I will advice against chamber/beam/sample geometry
change during troubleshooting process.

Now down to business with Ann's images. The sawtooth vertical edges
appearance is the result of horizontal scan being out of phase with the
external source of either vibration, fields, or signal interference. Time
delay between the lines at slow scan is in order of tens to hundreds
milliseconds, which is within the possible vibration frequency range. Same
frequencies will cause wavy image at TV deflection speeds.

Another point is that mechanical vibration in many cases repeats the AC line
frequency, as it is caused by electric motor driven devices, so either 60 Hz
or it's harmonic(s) are present as mechanical vibrations. The only way to
find the problem is to troubleshoot and differentiate.

I agree with other postings- more information needed to develop more
accurate idea on how to proceed.


Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: Allen Sampson {ars-at-sem.com}
To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
{yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, November 07, 2002 3:50 PM


Dear All,

How much is a reasonable price to pay for a Leica/Leo Stereoscan s440 and/
or what % of the original purchase price should one expect to pay for a ~6
year old SEM in good working order? I would be most grateful for your opinions.

best regards and thanks,

Marion



***********************************************
Dr Marion A. Stevens-Kalceff
Deputy Director
Electron Microscope Unit
University of New South Wales,
Sydney NSW 2052 Australia
***********************************************



From daemon Fri Nov 8 03:16:34 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 8 Nov 2002 08:53:22 -0000
Subject: Fw: Kodak Rapid Fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The exact concentrations of fixers are not very critical, so don't
lose sleep over these minor discrepancies.
Unlike developers where time, concentration and temperature must be
correct to get a reproducible result, you can
safely vary fixer concentrations over quite a wide range and fix for
the time/temperature required for completion.
A good rule of thumb is to fix for twice the time required to clear
the emulsion.
The yellow crystals are elemental sulphur, indicating that the
ammonium thiosulphate is decomposing.
The fixer may still work adequately if sufficient thiosulphate
remains. Agfa's tip is to compare the film-clearing time with
that of a fresh batch of fix. If it takes more than twice as long the
fixer is exhausted.
Chris

} ----- Original Message -----
} From: "Pat Connelly" {psconnel-at-sas.upenn.edu}
} To: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, November 07, 2002 9:29 PM
} Subject: Kodak Rapid Fixer
}
}

} --------------------------------------------------------------------
----
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} --------------------------------------------------------------------
---.
}
}
} Why is it that the directions from Kodak for making a gallon of
Rapid
} Fixer state that I should pour 32oz. of "A" and 104ml of "B" into
a
} half gallon of water and then make it up to 1 gal. but the kit to
} make 1 Gal. has 33oz of "A" and 106ml of "B"?
}
} Also for people who now seldom need to use fixer - be advised that
} "A" DOES go bad with age. You will know it when a precipitate
forms
} as "B" is added slowly to the mixture of water and "A". If the "A"
is
} really old there will be the signs of yellow crystals on the sides
of
} the container.
}
} I questioned Kodak representatives in the mid-1980's and again in
the
} 1990's and was told not to worry. I still worry!
}
} Pat Connelly
} Univ. of Pennsylvania
} Biology Dept.






From daemon Fri Nov 8 03:52:23 2002



From: Allen Sampson :      ars-at-sem.com
Date: Fri, 8 Nov 2002 03:43:13 -0800
Subject: RE: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Reminds me of the very old question of can a scanning electron spot on a
CRT exceed the speed of light?

If only it could be as easy to figure as you portray. I'll try to explain
the situation, but it is really more complex that I can describe in words
here - and being late at night, I really won't spend much time on the
matter.

Let's assume that we are working at a working distance of 20mm. For the
velocity you give for electrons accelerated at 2KV (27,000 kM/S) or 15KV
(73,000 kM/S), that 20mm would be traversed in an exceeding small fraction
of a second. However, we aren't looking at the spatial translation of a
single spot. In the SEM, the spot formed on the sample is constantly
moving - in the case of a record image the movement of the spot is
generally determined by the dwell time on each of the individual pixels on
each line and the number and resolution of lines in the frame.

In the record mode, most instruments will sync the start of each line to
the zero-crossing of the electrical mains. This is an intentional attempt
to minimize the effect of 50 or 60 Hz. interference. Digital systems may
or may not do this.

If you go vertically down the recorded image, you are actually looking at
pixels that are separated by fairly large periods of time, 1/50th or 1/60th
of a second in the case of a synched record cycle. Now let's look at your
projected travel times using these figures. The difference between the 2KV
and 15KV velocities you mention is 46000 kM/S. That gives us a difference
in the velocity at these two accelerating voltages of 4.35x10-9 seconds to
traverse the 20mm to the sample.

Assuming a 60 Hz system, the difference between two vertically separated
pixels is 0.0167 seconds. Let's say that each 1/60th of a second is split
into 1500 equal parts - 1000 pixels used per line and 500 portions used for
the vertical retrace. That means that each pixel would be 0.000011 seconds
apart.

Now let's look at two pixels that are on separate scan lines and separated
by one additional pixel. They would be separated by a time period of
0.016678 seconds (adding the pixel dwell time to the line dwell time).
Doesn't sound like much, but in EM we have to understand the effects of
the orders of magnitude we work with. If you divide the above time
difference to traverse the working distance by the pixel time difference
just mentioned, you would find that there is a 2.58x10-7 second difference
between the pixels mentioned above.

I'm tired and really don't want to extend this too much more, but consider
that the vibrations seen on the images at question cover around 20 or more
scan lines and figure in the velocities of a nanometer scale RMS vibration.
You'll find that there is indeed a considerable, measurable, variation in
image vibrations due to accelerating voltage (as well as working distance).

Amazing, isn't it?

By the way, I still claim that the frequency of the problems in the
original image is much lower than 50 or 60 Hertz and is the result of
vibrations rather than any electro-magnetic or electronic effect. Another
poster mentioned the possibility that a turbo pump may be having problems
but that is also not a likely cause as the frequencies involved don't
match. You might, however, want to check the operation of any water
chiller that is in use.

If I have learned anything in working on these instruments for well over
twenty years, it's that nothing is simple or straightforward. We're
dealing with sub-atomic particle physics here folks, an area that can only
be currently described by the statistical methods of quantum theories.

As far as I am concerned, there is no further determination to be made -
the problem is one of mechanical vibrations and I would be glad to stake my
reputation on it, as I do on any posting I make here.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Thursday, November 07, 2002 10:54 PM, Vitaly Feingold
[SMTP:vitalylazar-at-worldnet.att.net] wrote:
} Hey Allen,
}
} You may found the following figures entertaining. Natural
} constants and results of calculations are rounded to the first character
} after the decimal point, relativity effects are neglected, plain text
format
} is used. I am a bit rusty on my Physics- my apologies if I missed an
order
} or so of magnitude. Makes no difference in this example.
}
} Electron mass ~ 9.1 x 10-31kg
} Electron charge ~ -1.6 x 10-19 C
} One electronvolt energy ~ 1.6 x 10-19J
} Kinetic energy (of electron, or anything having mass) = mV2/2, where m is
} the mass,
} V2 is the velocity square.
}
} Simple calculations yield the following velocities in kilometers per
second
} for electrons accelerated by potential differences of:
} 1 Volt ~ 6 x 100 km/s
} 200 V ~ 8.4 x 1,000 km/s
} 2 kV ~ 2,7 x 10,000 km/s
} 15 kV ~ 7.3 x 10,000 km/s
}
} Amazing, isn't it?
}
} Mechanical vibration velocity must be compatible in magnitude with the
above
} velocities, in order for vibration effects to look different at different
} beam accelerating voltages. But SEM is made of the materials found on
this
} planet, with limited durability specifications. Thus, in order to vibrate
} with such velocities, SEM must be either made very tiny, which is not the
} case, or must disintegrate into dust, but it doesn't.
}
} Velocities of mechanical vibrations affecting SEM are in many orders of
} magnitude slower
} than the E-beam velocities.
}
} This is why SEM stage vibration will show up exactly the same at
different
} beam accelerating voltages.
}
} Why then stray magnetic field affects an e-beam? Because electron has
huge
} charge/mass ratio, ~ 10,000,000,000. The interaction is very strong.
Thus,
} effect increases at lower accelerating voltages. Same with working
distance-
} effect increases with the distance increase. But, one needs to change WD
} substantially, to see the effect for sure. That will reduce the
resolution,
} among other things. I will advice against chamber/beam/sample geometry
} change during troubleshooting process.
}
} Now down to business with Ann's images. The sawtooth vertical edges
} appearance is the result of horizontal scan being out of phase with the
} external source of either vibration, fields, or signal interference. Time
} delay between the lines at slow scan is in order of tens to hundreds
} milliseconds, which is within the possible vibration frequency range.
Same
} frequencies will cause wavy image at TV deflection speeds.
}
} Another point is that mechanical vibration in many cases repeats the AC
line
} frequency, as it is caused by electric motor driven devices, so either 60
Hz
} or it's harmonic(s) are present as mechanical vibrations. The only way to
} find the problem is to troubleshoot and differentiate.
}
} I agree with other postings- more information needed to develop more
} accurate idea on how to proceed.
}
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
}
} This address can not receive messages larger than 15 kb without prior
} notification.
}
} ----- Original Message -----
} From: Allen Sampson {ars-at-sem.com}
} To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
} {yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
} {Microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, November 07, 2002 3:50 PM
} Subject: RE: vibration?
}
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------
---.
} }
} }
} } Vitaly,
} }
} } Most of your concerns I covered in a previous reply. However, I have
to
} } take exception to your claim that a large change in the problem's
} } characteristic would result from a change in accelerating voltage only
if
} } the problem was electro-magnetic. A reduction in the accelerating
voltage
}
} } will also result in a reduction of the velocity of electrons in the
beam.
} } That would cause an increased displacement of the beam as it traverses
} the
} } sample surface due to vibrations.
} }
} } In other words, the effects of electro-magnetic interference and
} vibrations
} } are virtually inseparable except for the frequency involved. Each will
} } affect the beam in similar ways, in regards to accelerating voltage and
} } working distance. Both will have a greater affect with a lower
} } accelerating voltage as well as a greater working distance.
} }
} }
} } Allen R. Sampson
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} }
} } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
} }
} }
} } On Wednesday, November 06, 2002 8:58 PM, Vitaly Feingold
} } [SMTP:vitalylazar-at-worldnet.att.net] wrote:
} } } Could be vibration. Could also be magnetic field. And, could be
signal
} } } interference, such as ground loop. What type of SEM are you using?
} } }
} } } I will omit remedies for the vibration problem, since Allen Sampson
did
} } } pretty good job addressing that.
} } }
} } } Stray AC line frequency magnetic field: try acquiring image at
} different
} } } accelerating voltages. It is very important that all other geometry
} } } parameters will remain the same: particular area of a particular
} } specimen,
} } } WD, tilt, magnification, spot size. Contrast, brightness, focus, and
} } } stigmator settings can be changed. If the problem is much worse at,
say,
} } 2
} } } kV, than at, say, 15kV, you are dealing with magnetic field. You may
} also
} } } try to keep 3 axes AC milliGauss meter at the SEM, and notice what it
} } } measures, but catching stray magnetic interference this way may be a
} } little
} } } tricky. These meters, though, are very inexpensive, and available
from
} } many
} } } test instruments suppliers.
} } }
} } } Now we are getting down to statistically most likely problem- signal
} } } interference and ground loops. It is difficult to go any further
without
}
} } } knowing the SEM type, and acquisition system (if separate) type. Too
} many
} } } possibilities exist, but the very first- does your acquisition
software
} } } have an option somewhere in the settings which reads something like
"60
} } } Hertz sync", or "AC line synchronization", or "Line sync", etc.,
etc.?
} If
} } } yes, check (or uncheck) that option and see what happens. Further
} } remedies
} } } of this problem will include eliminating ground loops. Example- sh
ielded
} } } cable(s) has shield connected on both sides, and shield is used as
one
} of
} } } the signal wires. This is frequently done, and does jeopardize
} instrument
} } } interference tolerance. Another example- one of the accessories is
} } } susceptible to some kind of interference, and must be powered through
} the
} } } isolation transformer. The latter remedy will only work with decent
} } (better
} } } if dedicated) ground. The fact that the problem is not present all
the
} } time
} } } does not mean that everything is perfect inside the SEM. Besides,
going
} } } after the EM interference source could be inefficient and
frustrating,
} if
} } } not impossible. Improving grounding/shielding/power connection might
be
} } } easier.
} } }
} } }
} } } Vitaly Feingold
} } } Scientific Instruments and Applications
} } } 2773 Heath Lane, Duluth GA 30096
} } } (770)232-7785 ph.
} } } (770)232-1791 fax
} } } (678)467-0012 mobile
} } }
} } } This message is made of 100% recycled electrons.
} } }
} } } This address can not receive messages larger than 15 kb without prior
} } } notification.
} } }
} } } ----- Original Message -----
} } } } From: Ann-Fook Yang {yanga-at-agr.gc.ca}
} } } To: {microscopy-at-sparc5.microscopy.com}
} } } Sent: Wednesday, November 06, 2002 2:45 PM
} } } Subject: vibration?
} } }
} } }
} } } }
} }
------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} }
-----------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi everyone,
} } } }
} } } } I have two SEM micrographs on the web:
} } } } http://www.magma.ca/~scimat/Defect.htm
} } } } The micrographs show zagged edges taken at 20 kX. Such phenomenon
} does
} } } not present all the time. Can anyone suggest what causes the problem
and
} } how
} } } to solve it?
} } } } Thanking in advance.
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } } } AnnFook Yang
} } } } EM Unit,
} } } } Eastern Cereal and Oilseed Research Centre,
} } } } Room 2091, Bldg. 20,
} } } } Central Experimental Farm,
} } } } Ottawa, Ontario
} } } } Canada K1A 0C6
} } } }
} } } } Tel: 1-613-759-1638
} } } } Fax: 1-613-759-1701
} } } }
} } } } e-mail: yanga-at-em.agr.ca
} } } }
} } } }
} } } }
} } }
} } }
} } }
} } }
} } }
} }
} }
}
}
}



From daemon Fri Nov 8 06:47:15 2002



From: Li, Huilin :      hli-at-bnl.gov
Date: Fri, 8 Nov 2002 07:34:36 -0500
Subject: Postdoctoral Researcher Position at Brookhaven National Lab

Contents Retrieved from Microscopy Listserver Archives
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A postdoctoral researcher position is available to study the
structures of biological macromolecular complexes by electron
cryo-microscopy and image analysis. This position requires experience with
electron microscopy and image processing.

The Biology Department at Brookheaven National Laboratory (BNL)
has excellent facilities for cryo-EM and image processing. Major
facilities available include a new JEM 2010F FasTEM, and access to the STEM
facility and National Synchrotron Light Source.

Applicants should send in by email a CV, a brief statement of interests,
and names and addresses of 2-3 referees.

Huilin Li
Brookheaven National Laboratory
Biology Department, Bldg. 463
Upton, NY 11973
email: hli-at-bnl.gov
phone: (631)344-2931
fax: (631)344-3407


From daemon Fri Nov 8 07:33:56 2002



From: George Laing :      scisales-at-ngscorp.com
Date: Fri, 8 Nov 2002 08:26:07 -0500
Subject: RE: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Regal-Arkay and Jobo both offer "roller transport" type RC print dryers.
The Regal-Arkay units are high speed professional dryers that will last many
years. They are available in 12" and 20" widths.
Jobo sells DevAppa dryers in widths from 8" to 20". They are also very
good dryers but I do not have as much experience with them. Check them out
at http://www.jobo-usa.com/products/dry_prnt.htm
For more limited budgets, Premier has the RC-500A, which utilizes filtered
air blowing over prints. It will hold up to four 11x14 prints at one time.
The RC500A is under $300.
Of course, there is always the squeegee!!

George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com



Does anyone have tips on drying resin coated paper quickly???







From daemon Fri Nov 8 08:16:48 2002



From: Smartech :      smartech-at-optonline.net
Date: Fri, 08 Nov 2002 09:03:52 -0500
Subject: I need help with digital images __ How to subtract a gradaient from

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In doing SEM I occasionally acquire images that contain a gradient. One
example would be an x-ray map that I have acquired in an hours time.
Typically the gun will drift slightly out of alignment and the end of the
image is darker than the beginning. Another example is when I am collecting
very low magnification images using the conventional SEM detector (Everhart
and Thornley). The top left hand side of the image is much brighter than the
bottom right hand side. I would like a routine that averages pixel values,
say nearest ten neighbors, to create a new image based on just the averages.
I could then perhaps divide the original image by the average image and in
effect, normalize the original image.

It would be great if I could do this within Adobe Photoshop only.

Since it is SEM at least we are dealing with just 256 gray levels.

Any suggestions?

Ric Felten

SMARTech
860-485-5054
www.semguy.com
19 Cornwall Drive
Goshen CT 06756




From daemon Fri Nov 8 08:47:00 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 08 Nov 2002 09:39:21 -0500
Subject: Re: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
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Print dryers for resin coated papers are available, of course right now I can't
remember where! Try a google search for print dryers.

Geoff

Dean Abel wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Leslie,
} I use a Durst Laborator S-45 Special enlarger with a point light source
} and print all my TEM and 35mm negatives on Ilford Multigrade IV RC DeLuxe
} paper using Ilford Multigrade filters. It works fine for me and my bosses,
} but I am sure that other papers like Kodak Polymax II RC, as suggested by
} Karen Jensen, work just as well.
} I learned my darkroom work using fiber-based graded papers and drying them
} on a big mirrored drum, but now must I air dry my prints ever since our
} dryer broke and couldn't be fixed. This is a bit of a nuisance as the
} prints take overnight to dry and can't be turned in the same day they're
} printed.
} Does anyone have tips on drying resin coated paper quickly???
} Dean Abel
} Biological Sciences 138BB
} University of Iowa
} Iowa City IA 52242-1324

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Nov 8 08:50:02 2002



From: jerry smith :      jsmit51-at-tampabay.rr.com
Date: Fri, 8 Nov 2002 09:51:18 -0500
Subject: NEED LAB-6 EMITTER

Contents Retrieved from Microscopy Listserver Archives
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DOES ANYONE HAVE ANY GOOD USED
LAB-6 TIPS OR A SURPLUS NEW ONE THAT
WE COULD OBTAIN, TO COMPLETE A
PROJECT, WE HAVE ORDERED ONE BUT IT
TAKES 3 WEEKS TO GET. IT IS FOR AN
S570 HITACHI SEM.
PLEASE REPLY DIRECT TO E-MAIL

JERRY SMITH
JSMIT51-at-TAMPABAY.RR.COM



From daemon Fri Nov 8 09:06:06 2002



From: ramkik-at-wuphys.wustl.edu (by way of MicroscopyListserver)
Date: Fri, 8 Nov 2002 09:07:52 -0600
Subject: Ask-A-Microscopist: statistically justify the number of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


----- Original Message -----
} From: Allen Sampson {ars-at-sem.com}
To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
{yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
{Microscopy-at-sparc5.microscopy.com}
Sent: Friday, November 08, 2002 6:43 AM


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ramkik-at-wuphys.wustl.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on Friday,
November 8, 2002 at 08:48:27
---------------------------------------------------------------------------

Email: ramkik-at-wuphys.wustl.edu
Name: ramki kalyanaraman

Organization: Washington University in St. Louis

Education: Graduate College

Location: St. Louis, MO, USA

Question: Is there a way to statistically justify the number of TEM
samples that must be prepared to be confident. One answer to this may
be on the basis of the feature size beining invesitgated. There are
probably two extreme cases to base the answer upon: In one case we
have numerous independent prior measurements of the sample properties
while in the other we do not. If there are any good journal
paper/Book chapter that answers this question, I would appreciate
knowing about it.
thank you



---------------------------------------------------------------------------


From daemon Fri Nov 8 09:32:50 2002



From: Michael Urbanik :      crystalguru-at-earthlink.net
Date: Fri, 8 Nov 2002 07:16:59 -0500
Subject: I need lo res Silicon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Does anyone know where I can buy a small chunk of low resistivity ( {0.1
ohm-cm) Silicon for an experiment that I need to perform?
I need a crystal about 10 to 100 cc's in size and it could be a boule top or
bottom.
Cheers
Mike Urbanik
Florida



From daemon Fri Nov 8 10:26:48 2002



From: Shanling Shi :      Shanling.Shi-at-unilever.com
Date: Fri, 8 Nov 2002 11:17:39 -0500
Subject: Sectioning of Lowicryl K4M embedded sample

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,

Recently I freeze substitution embedded some skin samples with Lowicryl K4M.
The blocks were cured under UV at -35C for 2 days and at room temperature for
several days. The blocks looks fine to me after UV polymerization. However,
when I section the blocks, blocks surface always get wet and the section swell
badly on the water. It seems like sections dissolving into water. I couldn't
pick up any intact sections. I used Lowicryl HM 20 before. It worked very well
for me. I did read the instruction of Lowicryl resin and notice that the
sectioning polar resin is different from nonpolar resin. But I could not get
good sections of Lowicyl K4M. I don't know if the blocks are not fully
polymerized or I need some special tricks to get sections. Any suggestions are
really appreciated!

Shanling

Shanling Shi
Advanced Imaging & Measurement
Unilever Research & Development -Edgewater
45 River Road
Edgewater, NJ 07020
201-840-2340
Shanling.Shi-at-unilever.com








From daemon Fri Nov 8 10:38:08 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Fri, 08 Nov 2002 11:21:16 -0500
Subject: Re: Clinical turn around

Contents Retrieved from Microscopy Listserver Archives
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Sharon,
Two days seems very fast, type A personalities? Here we have a typical 1
week turnaround
time from fixation to sectioning (thick and thin) to viewing and scanning
negatives. I think that is a more
fair and resonable time frame for an 8 hour day.
Mike Delannoy
Johns Hopkins School of Medicine Microscope Facility

Ed Calomeni wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Morning Sharon,
}
} A typical turn -around time is 2-3 days, with four days being the latest
} that samples should be done by. These numbers come from many years of on
} the job numbers.
} Does your boss not help out at all in the lab?
} How many chucks for the microtome do you have? You should have at least 5,
} so you are not wasting time changing blocks all the time. Tissue processor?
} Film and paper processors?
}
} On average I was able to complete 2 (possibly 3) cases/day. Of course these
} are averages. Some times the do-do does hit the fan.
}
} Ed Calomeni
} currently unemployed EM tech
} ----- Original Message -----
} } From: "Sharon Drew (by way of MicroscopyListserver)" {drewsh-at-musc.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, November 06, 2002 5:26 PM
} Subject: Clinical turn around
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi!
} } I run a diagnostic TEM laboratory in SC.
} } One tech.
} } 6 to 5 samples every other day at least.
} } What is the turn around time asked for your labs out there that are
} } also doing diagnostic/clinical work and where does that number come
} } from?
} } Thank you for any help.
} } My supervisor is saying 2 days but I think that is close to impossible
} } when only one tech doing everything including transport of tissue from
} } another lab and scoping all case that come in.
} } Sharon Drew
} } Diagnostic Pathology EM
} } Charleston, SC
} }



From daemon Fri Nov 8 11:22:30 2002



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Fri, 08 Nov 2002 11:12:41 -0600
Subject: Re: Sectioning of Lowicryl K4M embedded sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shanling: You have discovered the well known downside of K4M but it can be
overcome. Half the battle is to get the water level just right. We also
find the sectioning characteristics (and I think how easily water jumps to
the surface) also improves if we store the blocks in a desiccator overnight
before we cut them. Don't use too slow of a cutting speed since that
promotes wetting of the surface. If you do get the block face wet, you
need to dry both the block face and back of the knife before re-starting or
it will wet immediately upon restarting. We prefer LR Gold for this reason
but Lowicryl K4M has some features that make it superior for some
antigens. Good luck.

At 11:17 AM 11/8/2002 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri Nov 8 11:54:08 2002



From: Baggethun, Paul :      Paul.Baggethun-at-alcoa.com
Date: Fri, 8 Nov 2002 12:47:58 -0500
Subject: I need help with digital images __ How to subtract a gradaient

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What SEM model is it?

Very generally, assuming that column/apertures alignment/centering is
correct- it could be a problem with, or an incorrect setting of the SED
collector grid voltage (likely), or collector grid could be damaged/dirty
(unlikely). Was this problem always there at low mag., or had it started
happening recently?

Outside fixing an actual problem- acquire and keep on file a reference image
of a perfectly smooth featureless surface (pick proper gain/black level
settings). Then run flat field correction routine on your real images, with
the use of that image file. Flat field correction can be found in a number
of image processing programs. This routine is used to correct vignetting
effect of an optical lens, among other things.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: Smartech {smartech-at-optonline.net}
To: To all on the list {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, November 08, 2002 9:03 AM


Remove the long-wavelength intensity fluctuation from your images using a
highpass Fourier Transform filter.

This, and other background correction utilities are available (for free) in
ImageJ http://rsb.info.nih.gov/ij/ ImageJ is the most versatile 2D ip&a
software for those hwo do not require super-high speed.

Cheers,
Paul Baggethun
Pittsburgh, PA

-----Original Message-----
} From: Smartech [mailto:smartech-at-optonline.net]
Sent: Friday, November 08, 2002 9:04 AM
To: To all on the list


In doing SEM I occasionally acquire images that contain a gradient. One
example would be an x-ray map that I have acquired in an hours time.
Typically the gun will drift slightly out of alignment and the end of the
image is darker than the beginning. Another example is when I am collecting
very low magnification images using the conventional SEM detector (Everhart
and Thornley). The top left hand side of the image is much brighter than the
bottom right hand side. I would like a routine that averages pixel values,
say nearest ten neighbors, to create a new image based on just the averages.
I could then perhaps divide the original image by the average image and in
effect, normalize the original image.

It would be great if I could do this within Adobe Photoshop only.

Since it is SEM at least we are dealing with just 256 gray levels.

Any suggestions?

Ric Felten

SMARTech
860-485-5054
www.semguy.com
19 Cornwall Drive
Goshen CT 06756




From daemon Fri Nov 8 12:04:37 2002



From: Holly Aaron :      hollya-at-socrates.berkeley.edu
Date: Fri, 8 Nov 2002 09:55:24 -0800
Subject: mercury lamp supplier

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Microscopists -

I'm looking for a good mercury bulb supplier? Any recommendations?
The last few I have gotten have not lasted the 200 hrs they should, so I am
looking for a new source.

Thank you in advance!
-Holly

Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California, Berkeley
447 Life Sciences Addition
Berkeley, CA 94720
http://imaging.berkeley.edu



From daemon Fri Nov 8 12:41:23 2002



From: S. Kuehner :      kuehner-at-u.washington.edu
Date: Fri, 8 Nov 2002 10:32:42 -0800 (PST)
Subject: Re: I need help with digital images __ How to subtract a gradaient

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The reason there is a gradient in your SE image is not due to any problems
with the column but is entirely due to the way the ET detector works.
The top left portion of your images are brighter because that is where
your ET detector is positioned (if it isn't, then there really is
something else wrong). The images are brighter at the upper left because
that part of the raster is closer to the detector and more secondary
electrons reach the detector, then the lower right, which is further away
from the detector. All side-mounted ET detectors (I think) show this at
low magnifications.

I think the easiest way to correct for this is the flat field method
suggested by Vitaly Feingold in the previous message.

************************************************

....amphiboles do violence to history...
T. Feininger, 2001. (taken out of context)
****************************

Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************

On Fri, 8 Nov 2002, Vitaly Feingold wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} What SEM model is it?
}
} Very generally, assuming that column/apertures alignment/centering is
} correct- it could be a problem with, or an incorrect setting of the SED
} collector grid voltage (likely), or collector grid could be damaged/dirty
} (unlikely). Was this problem always there at low mag., or had it started
} happening recently?
}
} Outside fixing an actual problem- acquire and keep on file a reference image
} of a perfectly smooth featureless surface (pick proper gain/black level
} settings). Then run flat field correction routine on your real images, with
} the use of that image file. Flat field correction can be found in a number
} of image processing programs. This routine is used to correct vignetting
} effect of an optical lens, among other things.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
}
} This address can not receive messages larger than 15 kb without prior
} notification.
}
} ----- Original Message -----
} } From: Smartech {smartech-at-optonline.net}
} To: To all on the list {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, November 08, 2002 9:03 AM
} Subject: I need help with digital images __ How to subtract a gradaient
} fromSEM image
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } In doing SEM I occasionally acquire images that contain a gradient. One
} } example would be an x-ray map that I have acquired in an hours time.
} } Typically the gun will drift slightly out of alignment and the end of the
} } image is darker than the beginning. Another example is when I am
} collecting
} } very low magnification images using the conventional SEM detector
} (Everhart
} } and Thornley). The top left hand side of the image is much brighter than
} the
} } bottom right hand side. I would like a routine that averages pixel
} values,
} } say nearest ten neighbors, to create a new image based on just the
} averages.
} } I could then perhaps divide the original image by the average image and in
} } effect, normalize the original image.
} }
} } It would be great if I could do this within Adobe Photoshop only.
} }
} } Since it is SEM at least we are dealing with just 256 gray levels.
} }
} } Any suggestions?
} }
} } Ric Felten
} }
} } SMARTech
} } 860-485-5054
} } www.semguy.com
} } 19 Cornwall Drive
} } Goshen CT 06756
} }
} }
} }
} }
}
}
}



From daemon Fri Nov 8 13:19:16 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 8 Nov 2002 11:04:50 -0800
Subject: oxygen monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

I am thinking I should be more careful with our liquid nitrogen handling. I
would like to monitor oxygen levels in the room we fill dewars.

So far, I have found small, battery operated low oxygen alarms for about
$300. But, they only last a year before needing replacement. The permanent
installations I have found go for over $1K.

Is this right? Any one with some sage advice? Yes, I know better safe than
sorry, but just double checking with the knowledge base.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Nov 8 13:25:45 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 08 Nov 2002 14:18:50 -0500
Subject: Re: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




One other possibility That we have run into recently is the interference
from a computer monitor that produces similar effects as shown in the
images. These effects usually are not noticed at low
magnifications...usually just when we are working at mags of 20,000x or
greater.
An easy way to check is just to turn off your computer monitor while you
are capturing an image. Wouldn't it be nice if the solution is as easy as
this!

Debby


Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907

On 11/7/02 3:50 PM, "Allen Sampson" {ars-at-sem.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Vitaly,
}
} Most of your concerns I covered in a previous reply. However, I have to
} take exception to your claim that a large change in the problem's
} characteristic would result from a change in accelerating voltage only if
} the problem was electro-magnetic. A reduction in the accelerating voltage
} will also result in a reduction of the velocity of electrons in the beam.
} That would cause an increased displacement of the beam as it traverses the
} sample surface due to vibrations.
}
} In other words, the effects of electro-magnetic interference and vibrations
} are virtually inseparable except for the frequency involved. Each will
} affect the beam in similar ways, in regards to accelerating voltage and
} working distance. Both will have a greater affect with a lower
} accelerating voltage as well as a greater working distance.
}
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} On Wednesday, November 06, 2002 8:58 PM, Vitaly Feingold
} [SMTP:vitalylazar-at-worldnet.att.net] wrote:
} } Could be vibration. Could also be magnetic field. And, could be signal
} } interference, such as ground loop. What type of SEM are you using?
} }
} } I will omit remedies for the vibration problem, since Allen Sampson did
} } pretty good job addressing that.
} }
} } Stray AC line frequency magnetic field: try acquiring image at different
} } accelerating voltages. It is very important that all other geometry
} } parameters will remain the same: particular area of a particular
} specimen,
} } WD, tilt, magnification, spot size. Contrast, brightness, focus, and
} } stigmator settings can be changed. If the problem is much worse at, say,
} 2
} } kV, than at, say, 15kV, you are dealing with magnetic field. You may also
} } try to keep 3 axes AC milliGauss meter at the SEM, and notice what it
} } measures, but catching stray magnetic interference this way may be a
} little
} } tricky. These meters, though, are very inexpensive, and available from
} many
} } test instruments suppliers.
} }
} } Now we are getting down to statistically most likely problem- signal
} } interference and ground loops. It is difficult to go any further without
} } knowing the SEM type, and acquisition system (if separate) type. Too many
} } possibilities exist, but the very first- does your acquisition software
} } have an option somewhere in the settings which reads something like "60
} } Hertz sync", or "AC line synchronization", or "Line sync", etc., etc.? If
} } yes, check (or uncheck) that option and see what happens. Further
} remedies
} } of this problem will include eliminating ground loops. Example- shielded
} } cable(s) has shield connected on both sides, and shield is used as one of
} } the signal wires. This is frequently done, and does jeopardize instrument
} } interference tolerance. Another example- one of the accessories is
} } susceptible to some kind of interference, and must be powered through the
} } isolation transformer. The latter remedy will only work with decent
} (better
} } if dedicated) ground. The fact that the problem is not present all the
} time
} } does not mean that everything is perfect inside the SEM. Besides, going
} } after the EM interference source could be inefficient and frustrating, if
} } not impossible. Improving grounding/shielding/power connection might be
} } easier.
} }
} }
} } Vitaly Feingold
} } Scientific Instruments and Applications
} } 2773 Heath Lane, Duluth GA 30096
} } (770)232-7785 ph.
} } (770)232-1791 fax
} } (678)467-0012 mobile
} }
} } This message is made of 100% recycled electrons.
} }
} } This address can not receive messages larger than 15 kb without prior
} } notification.
} }
} } ----- Original Message -----
} } } From: Ann-Fook Yang {yanga-at-agr.gc.ca}
} } To: {microscopy-at-sparc5.microscopy.com}
} } Sent: Wednesday, November 06, 2002 2:45 PM
} } Subject: vibration?
} }
} }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Hi everyone,
} } }
} } } I have two SEM micrographs on the web:
} } } http://www.magma.ca/~scimat/Defect.htm
} } } The micrographs show zagged edges taken at 20 kX. Such phenomenon does
} } not present all the time. Can anyone suggest what causes the problem and
} how
} } to solve it?
} } } Thanking in advance.
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } } AnnFook Yang
} } } EM Unit,
} } } Eastern Cereal and Oilseed Research Centre,
} } } Room 2091, Bldg. 20,
} } } Central Experimental Farm,
} } } Ottawa, Ontario
} } } Canada K1A 0C6
} } }
} } } Tel: 1-613-759-1638
} } } Fax: 1-613-759-1701
} } }
} } } e-mail: yanga-at-em.agr.ca
} } }
} } }
} } }
} }
} }
} }
} }
} }
}
}
}



From daemon Fri Nov 8 14:03:24 2002



From: Everett Ramer :      eramer-at-cellomics.com
Date: Fri, 8 Nov 2002 14:52:42 -0500
Subject: RE: Ask-A-Microscopist: statistically justify the number of TEM s

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You will find a good description of how to use a pilot study to determine
the number of samples in:
* Chapter 10 of Unbiased Stereology by C.V. Howard and M.G. Reed
* Stereological Methods Vol 1: Practical Methods for Biological
Morphometry by E.R. Weibel.
Everett Ramer
Cellomics, Inc.
Pittsburgh, PA



From daemon Fri Nov 8 14:03:25 2002



From: Tamara Howard :      thoward-at-unm.edu
Date: Fri, 8 Nov 2002 12:49:55 -0700 (MST)
Subject: Re: Sectioning of Lowicryl K4M embedded sample

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This may help (may not, though!) - try storing the blocks in a desiccator
with dry-rite or some other desiccant when you aren't using them. I've had
similar trouble with K4M blocks that were made during humid weather -
drying them out helped. Sometimes these resins are a little too
hydrophilic!

Tamara

On Fri, 8 Nov 2002, Shanling Shi wrote:

} ------------------------------------------------------------------------
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}
}
} Dear listers,
}
} Recently I freeze substitution embedded some skin samples with Lowicryl K4M.
} The blocks were cured under UV at -35C for 2 days and at room temperature for
} several days. The blocks looks fine to me after UV polymerization. However,
} when I section the blocks, blocks surface always get wet and the section swell
} badly on the water. It seems like sections dissolving into water. I couldn't
} pick up any intact sections. I used Lowicryl HM 20 before. It worked very well
} for me. I did read the instruction of Lowicryl resin and notice that the
} sectioning polar resin is different from nonpolar resin. But I could not get
} good sections of Lowicyl K4M. I don't know if the blocks are not fully
} polymerized or I need some special tricks to get sections. Any suggestions are
} really appreciated!
}
} Shanling
}
} Shanling Shi
} Advanced Imaging & Measurement
} Unilever Research & Development -Edgewater
} 45 River Road
} Edgewater, NJ 07020
} 201-840-2340
} Shanling.Shi-at-unilever.com
}
}
}
}
}
}
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Fri Nov 8 14:39:07 2002



From: Allen R. Sampson :      ars-at-sem.com
Date: Fri, 8 Nov 2002 14:29:16 -0800
Subject: RE: vibration?

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My apologies to all - I made a couple of postings on this matter. The
first laid down the conditions I was using, assumptions that had to be made
(I should have just asked the original poster, but what I wrote was
intended to be more general in application).

That original reply of mine laid out the assumption that the images were
long exposures. All later remarks I made were based on that. If, instead,
these were short exposures then the frequencies involved could be 50 or 60
Hz. or higher. My claim that these image problems would be vibrations was
based on those conditions.

Sorry if I caused any confusion on that point by not reiterating the
conditions in later messages..

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
ph. (630) 513-7093 fax (630) 513-7092 web http://www.sem.com


On Friday, November 08, 2002 6:57 AM, Vitaly Feingold
[SMTP:vitalylazar-at-worldnet.att.net] wrote:
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} -----------------------------------------------------------------------.
}
}
} ----- Original Message -----
} } From: Allen Sampson {ars-at-sem.com}
} To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
} {yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
} {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, November 08, 2002 6:43 AM
} Subject: RE: vibration?
}
}
} } Reminds me of the very old question of can a scanning electron spot on
a
} } CRT exceed the speed of light?
} }
} } If only it could be as easy to figure as you portray. I'll try to
explain
} } the situation, but it is really more complex that I can describe in
words
} } here - and being late at night, I really won't spend much time on the
} } matter.
} }
} } Let's assume that we are working at a working distance of 20mm. For
the
} } velocity you give for electrons accelerated at 2KV (27,000 kM/S) or
15KV
} } (73,000 kM/S), that 20mm would be traversed in an exceeding small
fraction
} } of a second. However, we aren't looking at the spatial translation of
a
} } single spot. In the SEM, the spot formed on the sample is constantly
} } moving - in the case of a record image the movement of the spot is
} } generally determined by the dwell time on each of the individual pixels
on
} } each line and the number and resolution of lines in the frame.
} }
} } In the record mode, most instruments will sync the start of each line
to
} } the zero-crossing of the electrical mains. This is an intentional
attempt
} } to minimize the effect of 50 or 60 Hz. interference. Digital systems
may
} } or may not do this.
} }
} } If you go vertically down the recorded image, you are actually looking
at
} } pixels that are separated by fairly large periods of time, 1/50th or
} 1/60th
} } of a second in the case of a synched record cycle. Now let's look at
your
} } projected travel times using these figures. The difference between the
} 2KV
} } and 15KV velocities you mention is 46000 kM/S. That gives us a
difference
} } in the velocity at these two accelerating voltages of 4.35x10-9 seconds
to
} } traverse the 20mm to the sample.
} }
}
} Correct.
}
}
} } Assuming a 60 Hz system, the difference between two vertically
separated
} } pixels is 0.0167 seconds.
}
}
} That is correct for a scan speed of 60 lines per second - frame of 1000
} lines will be scanned in 16 seconds.
}
}
} } Let's say that each 1/60th of a second is split
} } into 1500 equal parts - 1000 pixels used per line and 500 portions used
} for
} } the vertical retrace. That means that each pixel would be 0.000011
} seconds
} } apart.
}
}
} Adjacent horizontal pixels will be ~ 0.00001 seconds apart. While
adjacent
} vertical pixels will be 1/60s ~ 0.016s apart.
}
}
} } Now let's look at two pixels that are on separate scan lines and
separated
} } by one additional pixel. They would be separated by a time period of
} } 0.016678 seconds (adding the pixel dwell time to the line dwell time).
}
}
} Correct.
}
}
} } Doesn't sound like much, but in EM we have to understand the effects
of
} } the orders of magnitude we work with. If you divide the above time
} } difference to traverse the working distance by the pixel time
difference
} } just mentioned, you would find that there is a 2.58x10-7 second
difference
} } between the pixels mentioned above.
}
}
} Here is the juice. When you divide a time period by a time period, the
} result
} can not be time. It can be, however, a number of events, a number of
} portions, etc. What portions? The above figure of 2.58x10-7 is the
} difference
} between the portions of a pixel, which beam will scan across
} the specimen surface, equal to the time difference between 2kV and 15kV
} electrons crossing of the WD of 20 mm. Beam deflection (scan) times
remain
} the same for both accelerating voltages, of course.
} Can you see such difference on a micrograph? The ~ 3/10,000,000 of a
pixel?
} This will be the difference attributed to accelerating voltage change
from
} 2kV to 15kV and vice versa. This is why kV change will not bring any
} noticeable change into vibration affected image.
}
} My whole point was that scan (deflection) times/speeds are compatible
with
} times/speeds of both mechanical and AC magnetic
} events. The electron velocities/timing, in contrary, are not. They are
many
} orders of
} magnitude shorter/faster than mechanical events.
}
}
} }
} } I'm tired and really don't want to extend this too much more, but
consider
} } that the vibrations seen on the images at question cover around 20 or
more
} } scan lines and figure in the velocities of a nanometer scale RMS
} vibration.
} } You'll find that there is indeed a considerable, measurable, variation
in
} } image vibrations due to accelerating voltage (as well as working
} distance).
} }
} } Amazing, isn't it?
} }
} } By the way, I still claim that the frequency of the problems in the
} } original image is much lower than 50 or 60 Hertz and is the result of
} } vibrations rather than any electro-magnetic or electronic effect.
Another
} } poster mentioned the possibility that a turbo pump may be having
problems
} } but that is also not a likely cause as the frequencies involved don't
} } match. You might, however, want to check the operation of any water
} } chiller that is in use.
}
} Perhaps so, perhaps not. It can be chiller, or turbo pump, or anything
else.
} I can't say without more information.
}
}
} }
} } If I have learned anything in working on these instruments for well
over
} } twenty years, it's that nothing is simple or straightforward. We're
} } dealing with sub-atomic particle physics here folks, an area that can
only
} } be currently described by the statistical methods of quantum theories.
} }
} } As far as I am concerned, there is no further determination to be made
-
} } the problem is one of mechanical vibrations and I would be glad to
stake
} my
} } reputation on it, as I do on any posting I make here.
} }
} }
} } Allen R. Sampson
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} }
} } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
}
} This address can not receive messages larger than 15 kb without prior
} notification.
}
}
} }
} }
} } On Thursday, November 07, 2002 10:54 PM, Vitaly Feingold
} } [SMTP:vitalylazar-at-worldnet.att.net] wrote:
} } } Hey Allen,
} } }
} } } You may found the following figures entertaining. Natural
} } } constants and results of calculations are rounded to the first
character
} } } after the decimal point, relativity effects are neglected, plain text
} } format
} } } is used. I am a bit rusty on my Physics- my apologies if I missed an
} } order
} } } or so of magnitude. Makes no difference in this example.
} } }
} } } Electron mass ~ 9.1 x 10-31kg
} } } Electron charge ~ -1.6 x 10-19 C
} } } One electronvolt energy ~ 1.6 x 10-19J
} } } Kinetic energy (of electron, or anything having mass) = mV2/2, where
m
} is
} } } the mass,
} } } V2 is the velocity square.
} } }
} } } Simple calculations yield the following velocities in kilometers per
} } second
} } } for electrons accelerated by potential differences of:
} } } 1 Volt ~ 6 x 100 km/s
} } } 200 V ~ 8.4 x 1,000 km/s
} } } 2 kV ~ 2,7 x 10,000 km/s
} } } 15 kV ~ 7.3 x 10,000 km/s
} } }
} } } Amazing, isn't it?
} } }
} } } Mechanical vibration velocity must be compatible in magnitude with
the
} } above
} } } velocities, in order for vibration effects to look different at
} different
} } } beam accelerating voltages. But SEM is made of the materials found on
} } this
} } } planet, with limited durability specifications. Thus, in order to
} vibrate
} } } with such velocities, SEM must be either made very tiny, which is not
} the
} } } case, or must disintegrate into dust, but it doesn't.
} } }
} } } Velocities of mechanical vibrations affecting SEM are in many orders
of
} } } magnitude slower
} } } than the E-beam velocities.
} } }
} } } This is why SEM stage vibration will show up exactly the same at
} } different
} } } beam accelerating voltages.
} } }
} } } Why then stray magnetic field affects an e-beam? Because electron has
} } huge
} } } charge/mass ratio, ~ 10,000,000,000. The interaction is very strong.
} } Thus,
} } } effect increases at lower accelerating voltages. Same with working
} } distance-
} } } effect increases with the distance increase. But, one needs to change
WD
} } } substantially, to see the effect for sure. That will reduce the
} } resolution,
} } } among other things. I will advice against chamber/beam/sample
geometry
} } } change during troubleshooting process.
} } }
} } } Now down to business with Ann's images. The sawtooth vertical edges
} } } appearance is the result of horizontal scan being out of phase with
the
} } } external source of either vibration, fields, or signal interference.
} Time
} } } delay between the lines at slow scan is in order of tens to hundreds
} } } milliseconds, which is within the possible vibration frequency range.
} } Same
} } } frequencies will cause wavy image at TV deflection speeds.
} } }
} } } Another point is that mechanical vibration in many cases repeats the
AC
} } line
} } } frequency, as it is caused by electric motor driven devices, so
either
} 60
} } Hz
} } } or it's harmonic(s) are present as mechanical vibrations. The only
way
} to
} } } find the problem is to troubleshoot and differentiate.
} } }
} } } I agree with other postings- more information needed to develop more
} } } accurate idea on how to proceed.
} } }
} } }
} } } Vitaly Feingold
} } } Scientific Instruments and Applications
} } } 2773 Heath Lane, Duluth GA 30096
} } } (770)232-7785 ph.
} } } (770)232-1791 fax
} } } (678)467-0012 mobile
} } }
} } } This message is made of 100% recycled electrons.
} } }
} } } This address can not receive messages larger than 15 kb without prior
} } } notification.
} } }
} } } ----- Original Message -----
} } } From: Allen Sampson {ars-at-sem.com}
} } } To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
} } } {yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
} } } {Microscopy-at-sparc5.microscopy.com}
} } } Sent: Thursday, November 07, 2002 3:50 PM
} } } Subject: RE: vibration?
} } }
} } }
} } } }
} } --------------------------------------------------------------------
----
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
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} } ListServer-at-MSA.Microscopy.Com
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} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
--------------------------------------------------------------------
} } ---.
} } } }
} } } }
} } } } Vitaly,
} } } }
} } } } Most of your concerns I covered in a previous reply. However, I
have
} } to
} } } } take exception to your claim that a large change in the problem's
} } } } characteristic would result from a change in accelerating voltage
only
} } if
} } } } the problem was electro-magnetic. A reduction in the accelerating
} } voltage
} } }
} } } } will also result in a reduction of the velocity of electrons in the
} } beam.
} } } } That would cause an increased displacement of the beam as it
} traverses
} } } the
} } } } sample surface due to vibrations.
} } } }
} } } } In other words, the effects of electro-magnetic interference and
} } } vibrations
} } } } are virtually inseparable except for the frequency involved. Each
} will
} } } } affect the beam in similar ways, in regards to accelerating voltage
} and
} } } } working distance. Both will have a greater affect with a lower
} } } } accelerating voltage as well as a greater working distance.
} } } }
} } } }
} } } } Allen R. Sampson
} } } } Advanced Research Systems
} } } } 317 North 4th. Street
} } } } St. Charles, Illinois 60174
} } } }
} } } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
} } } }
} } } }
} } } } On Wednesday, November 06, 2002 8:58 PM, Vitaly Feingold
} } } } [SMTP:vitalylazar-at-worldnet.att.net] wrote:
} } } } } Could be vibration. Could also be magnetic field. And, could be
} } signal
} } } } } interference, such as ground loop. What type of SEM are you
using?
} } } } }
} } } } } I will omit remedies for the vibration problem, since Allen
Sampson
} } did
} } } } } pretty good job addressing that.
} } } } }
} } } } } Stray AC line frequency magnetic field: try acquiring image at
} } } different
} } } } } accelerating voltages. It is very important that all other
geometry
} } } } } parameters will remain the same: particular area of a particular
} } } } specimen,
} } } } } WD, tilt, magnification, spot size. Contrast, brightness, focus,
and
} } } } } stigmator settings can be changed. If the problem is much worse
at,
} } say,
} } } } 2
} } } } } kV, than at, say, 15kV, you are dealing with magnetic field. You
may
} } } also
} } } } } try to keep 3 axes AC milliGauss meter at the SEM, and notice
what
} it
} } } } } measures, but catching stray magnetic interference this way may
be a
} } } } little
} } } } } tricky. These meters, though, are very inexpensive, and available
} } from
} } } } many
} } } } } test instruments suppliers.
} } } } }
} } } } } Now we are getting down to statistically most likely problem-
signal
} } } } } interference and ground loops. It is difficult to go any further
} } without
} } }
} } } } } knowing the SEM type, and acquisition system (if separate) type.
Too
} } } many
} } } } } possibilities exist, but the very first- does your acquisition
} } software
} } } } } have an option somewhere in the settings which reads something
like
} } "60
} } } } } Hertz sync", or "AC line synchronization", or "Line sync", etc.,
} } etc.?
} } } If
} } } } } yes, check (or uncheck) that option and see what happens. Further
} } } } remedies
} } } } } of this problem will include eliminating ground loops. Example-
sh
} } ielded
} } } } } cable(s) has shield connected on both sides, and shield is used
as
} } one
} } } of
} } } } } the signal wires. This is frequently done, and does jeopardize
} } } instrument
} } } } } interference tolerance. Another example- one of the accessories
is
} } } } } susceptible to some kind of interference, and must be powered
} through
} } } the
} } } } } isolation transformer. The latter remedy will only work with
decent
} } } } (better
} } } } } if dedicated) ground. The fact that the problem is not present
all
} } the
} } } } time
} } } } } does not mean that everything is perfect inside the SEM. Besides,
} } going
} } } } } after the EM interference source could be inefficient and
} } frustrating,
} } } if
} } } } } not impossible. Improving grounding/shielding/power connection
might
} } be
} } } } } easier.
} } } } }
} } } } }
} } } } } Vitaly Feingold
} } } } } Scientific Instruments and Applications
} } } } } 2773 Heath Lane, Duluth GA 30096
} } } } } (770)232-7785 ph.
} } } } } (770)232-1791 fax
} } } } } (678)467-0012 mobile
} } } } }
} } } } } This message is made of 100% recycled electrons.
} } } } }
} } } } } This address can not receive messages larger than 15 kb without
} prior
} } } } } notification.
} } } } }
} } } } } ----- Original Message -----
} } } } } } From: Ann-Fook Yang {yanga-at-agr.gc.ca}
} } } } } To: {microscopy-at-sparc5.microscopy.com}
} } } } } Sent: Wednesday, November 06, 2002 2:45 PM
} } } } } Subject: vibration?
} } } } }
} } } } }
} } } } } }
} } } }
} }
------------------------------------------------------------------------
} } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
of
} } } } America
} } } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } }
} } } }
} }
-----------------------------------------------------------------------.
} } } } } }
} } } } } }
} } } } } } Hi everyone,
} } } } } }
} } } } } } I have two SEM micrographs on the web:
} } } } } } http://www.magma.ca/~scimat/Defect.htm
} } } } } } The micrographs show zagged edges taken at 20 kX. Such
phenomenon
} } } does
} } } } } not present all the time. Can anyone suggest what causes the
problem
} } and
} } } } how
} } } } } to solve it?
} } } } } } Thanking in advance.
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } } AnnFook Yang
} } } } } } EM Unit,
} } } } } } Eastern Cereal and Oilseed Research Centre,
} } } } } } Room 2091, Bldg. 20,
} } } } } } Central Experimental Farm,
} } } } } } Ottawa, Ontario
} } } } } } Canada K1A 0C6
} } } } } }
} } } } } } Tel: 1-613-759-1638
} } } } } } Fax: 1-613-759-1701
} } } } } }
} } } } } } e-mail: yanga-at-em.agr.ca
} } } } } }
} } } } } }
} } } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } }
} } } }
} } }
} } }
} } }
} }
}
}
}
}
}
}
}



From daemon Fri Nov 8 15:08:50 2002



From: DrJohnRuss-at-aol.com
Date: Fri, 8 Nov 2002 16:01:20 EST
Subject: Re: I need help with digital images __ How to subtract a gradaient from SEM image

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You can do what you describe in Photoshop by performing a Gaussian blur with
a large radius, to create a background. But you will probably find that you
get better results by using the darkest rather than the average of the pixels
in the neighborhood (you can do that, too - select Minimum) to create the
background. And for even more control, such as fitting a polynomial
background and either subtracting or dividing it, the Image Processing Tool
Kit plugins for Photoshop (and compatible programs) provides a variety of
background leveling options (the tutorial at http://ReindeerGraphics.com has
several examples).

John Russ


In a message dated 11/8/02 9:26:12 AM, smartech-at-optonline.net writes:

} In doing SEM I occasionally acquire images that contain a gradient. One
} example would be an x-ray map that I have acquired in an hours time.
} Typically the gun will drift slightly out of alignment and the end of the
} image is darker than the beginning. Another example is when I am collecting
} very low magnification images using the conventional SEM detector (Everhart
} and Thornley). The top left hand side of the image is much brighter than
} the bottom right hand side. I would like a routine that averages pixel
values,
} say nearest ten neighbors, to create a new image based on just the averages.
} I could then perhaps divide the original image by the average image and
} in effect, normalize the original image.
}
} It would be great if I could do this within Adobe Photoshop only.
}
} Since it is SEM at least we are dealing with just 256 gray levels.
}
} Any suggestions?


From daemon Fri Nov 8 15:28:30 2002



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Fri, 08 Nov 2002 13:20:27 -0800
Subject: RE: I need help with digital images __ How to subtract a gradaien t

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A simple recipe I use for leveling shading of SEM/TEM images and diffraction
patterns in Photoshop follows:

1. Open image.
2. Duplicate layer (twice, to keep unaltered original).
3. Apply Gaussian Blur filter to top layer. Start with about a 25 pixel
blur radius and adjust to get desired effect.
4. Invert image contrast
5. Select Overlay mode in Layers.
6. Merge Down (once).
7. Repeat process 2 or 3 times as appropriate. (Record as Action).

This method works best with images having no abrupt brightness transitions
(such as annotations).
Thanks to whoever first showed me this gem.

Larry Thomas
Pacific Northwest National Laboratory
P.O. Box 999
Mail Stop P8-16
Richland, WA, USA

email: Larry.Thomas-at-pnl.gov
phone: (509) 372-0793
fax; (509) 376-6308



} ----------
} From: Baggethun, Paul
} Sent: Friday, November 8, 2002 9:47 AM
} To: Microscopy-at-Sparc5. Microscopy. Com (E-mail)
} Subject: RE: I need help with digital images __ How to subtract a
} gradaien t from SEM image
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Remove the long-wavelength intensity fluctuation from your images using a
} highpass Fourier Transform filter.
}
} This, and other background correction utilities are available (for free)
} in
} ImageJ http://rsb.info.nih.gov/ij/ ImageJ is the most versatile 2D ip&a
} software for those hwo do not require super-high speed.
}
} Cheers,
} Paul Baggethun
} Pittsburgh, PA
}
} -----Original Message-----
} } From: Smartech [mailto:smartech-at-optonline.net]
} Sent: Friday, November 08, 2002 9:04 AM
} To: To all on the list
} Subject: I need help with digital images __ How to subtract a gradaient
} from SEM image
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} In doing SEM I occasionally acquire images that contain a gradient. One
} example would be an x-ray map that I have acquired in an hours time.
} Typically the gun will drift slightly out of alignment and the end of the
} image is darker than the beginning. Another example is when I am
} collecting
} very low magnification images using the conventional SEM detector
} (Everhart
} and Thornley). The top left hand side of the image is much brighter than
} the
} bottom right hand side. I would like a routine that averages pixel
} values,
} say nearest ten neighbors, to create a new image based on just the
} averages.
} I could then perhaps divide the original image by the average image and in
} effect, normalize the original image.
}
} It would be great if I could do this within Adobe Photoshop only.
}
} Since it is SEM at least we are dealing with just 256 gray levels.
}
} Any suggestions?
}
} Ric Felten
}
} SMARTech
} 860-485-5054
} www.semguy.com
} 19 Cornwall Drive
} Goshen CT 06756
}
}
}
}


From daemon Fri Nov 8 16:39:44 2002



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Fri, 8 Nov 2002 17:26:51 -0500
Subject: oxygen monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jonathan,
The least expensive unit I found about 1 1/2 years ago was an AirAware O2
monitor, #OF-67816 from Lab Safety Supply, 800 356-0783. It was twice what
I hoped to pay but much less than $1K. AC adaptor included.
Jim
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
354 Mansfield Road
Beach Hall, Room 129
Storrs, CT 06269-2242

860 486-2914 voice
860 486-6369 fax
james.romanow-at-uconn.edu




From daemon Fri Nov 8 18:30:27 2002



From: BALDWIN :      onubald-at-ecplaza.net
Date: Sat, 9 Nov 2002 01:22:07 +0100
Subject: PROJECT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friend,
I am Mr Onuigbo Baldwin Gozie, Bank Manager of Diamond BANK , Lagos
Branch. I have urgent and very confidential business proposition for you Mr. Barry Kelly made a numbered
time (Fixed) deposited for twelve calendar months, valued at
US$25,000,000.00 (Twenty-five Million Dollars) in my branch. Upon
maturity, I sent a routine notification to his forwarding address but got
no reply. After a month, we sent a reminder and finally we discovered from
his contract employers, Nigerian National Petroleum Corporation that Mr.
Barry Kelly died from an automobile accident. On further investigation, I
found out that he did not leave a WILL and all attempts to trace his next
of kin were fruitless. I therefore made further investigation and
discovered that Mr. Barry Kelly did not declare any next of kin in all his
official documents, including his Bank Deposit paperwork. This sum of
US$25,000,000.00 is still sitting in the Bank and the interest is being
rolled over with the principal sum at the end of each year. No one will
come forward to claim it. According to the Nigerian Law, at the expiration
of 6{Six} years, the money will revert to the ownership of the Nigerian
Government if nobody applies to claim the funds. Consequently, my proposal
is that I will like you as a foreigner to stand in as the next of kin to
Mr. Barry Kelly so that the fruits of this old man's labor will not get
into the hands of some corrupt government officials. This is simple;
1) I will like you to provide me immediately with your full names and
address so that the attorney will prepare the necessary documents and
affidavits, which will put you in place as the next of kin.
2) We shall employ the services of two attorneys for drafting and
notarization of the WILL and obtain the necessary documents and letter of
probate/administration in your favor for the transfer.
3) A bank account in any part of the world, which you provide, will then
facilitate the transfer of this money to you as the beneficiary/next of
kin of Mr.Barry Kelly. The money will be paid into your account for us to
share in the ratio of 75% for me and 20% for you then 5% will be set aside for any expenses that may occure during the transfer process.
There is no risk at all as all the paperwork for this transaction will be
done by the attorney and my position as the Branch Manager guarantees the
successful execution of this transaction. If you are interested, please
reply immediately via the private email address below. Upon your response,
I shall then provide you with more details and relevant documents that
will help you understand. Please observe utmost confidentiality, and rest
assured that this transaction would be most profitable for both of us
because I shall require your assistance to invest my share in your
country. Awaiting your urgent reply via email or my private telephone 234-1-7763642.
Thanks and regards
Baldwin Gozie Esq.








From daemon Fri Nov 8 18:30:28 2002



From: BALDWIN :      onubald-at-ecplaza.net
Date: Sat, 9 Nov 2002 01:21:48 +0100
Subject: PROJECT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friend,
I am Mr Onuigbo Baldwin Gozie, Bank Manager of Diamond BANK , Lagos
Branch. I have urgent and very confidential business proposition for you Mr. Barry Kelly made a numbered
time (Fixed) deposited for twelve calendar months, valued at
US$25,000,000.00 (Twenty-five Million Dollars) in my branch. Upon
maturity, I sent a routine notification to his forwarding address but got
no reply. After a month, we sent a reminder and finally we discovered from
his contract employers, Nigerian National Petroleum Corporation that Mr.
Barry Kelly died from an automobile accident. On further investigation, I
found out that he did not leave a WILL and all attempts to trace his next
of kin were fruitless. I therefore made further investigation and
discovered that Mr. Barry Kelly did not declare any next of kin in all his
official documents, including his Bank Deposit paperwork. This sum of
US$25,000,000.00 is still sitting in the Bank and the interest is being
rolled over with the principal sum at the end of each year. No one will
come forward to claim it. According to the Nigerian Law, at the expiration
of 6{Six} years, the money will revert to the ownership of the Nigerian
Government if nobody applies to claim the funds. Consequently, my proposal
is that I will like you as a foreigner to stand in as the next of kin to
Mr. Barry Kelly so that the fruits of this old man's labor will not get
into the hands of some corrupt government officials. This is simple;
1) I will like you to provide me immediately with your full names and
address so that the attorney will prepare the necessary documents and
affidavits, which will put you in place as the next of kin.
2) We shall employ the services of two attorneys for drafting and
notarization of the WILL and obtain the necessary documents and letter of
probate/administration in your favor for the transfer.
3) A bank account in any part of the world, which you provide, will then
facilitate the transfer of this money to you as the beneficiary/next of
kin of Mr.Barry Kelly. The money will be paid into your account for us to
share in the ratio of 75% for me and 20% for you then 5% will be set aside for any expenses that may occure during the transfer process.
There is no risk at all as all the paperwork for this transaction will be
done by the attorney and my position as the Branch Manager guarantees the
successful execution of this transaction. If you are interested, please
reply immediately via the private email address below. Upon your response,
I shall then provide you with more details and relevant documents that
will help you understand. Please observe utmost confidentiality, and rest
assured that this transaction would be most profitable for both of us
because I shall require your assistance to invest my share in your
country. Awaiting your urgent reply via email or my private telephone 234-1-7763642.
Thanks and regards
Baldwin Gozie Esq.








From daemon Fri Nov 8 18:30:28 2002



From: BALDWIN :      onubald-at-ecplaza.net
Date: Sat, 9 Nov 2002 01:20:16 +0100
Subject: PROJECT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friend,
I am Mr Onuigbo Baldwin Gozie, Bank Manager of Diamond BANK , Lagos
Branch. I have urgent and very confidential business proposition for you Mr. Barry Kelly made a numbered
time (Fixed) deposited for twelve calendar months, valued at
US$25,000,000.00 (Twenty-five Million Dollars) in my branch. Upon
maturity, I sent a routine notification to his forwarding address but got
no reply. After a month, we sent a reminder and finally we discovered from
his contract employers, Nigerian National Petroleum Corporation that Mr.
Barry Kelly died from an automobile accident. On further investigation, I
found out that he did not leave a WILL and all attempts to trace his next
of kin were fruitless. I therefore made further investigation and
discovered that Mr. Barry Kelly did not declare any next of kin in all his
official documents, including his Bank Deposit paperwork. This sum of
US$25,000,000.00 is still sitting in the Bank and the interest is being
rolled over with the principal sum at the end of each year. No one will
come forward to claim it. According to the Nigerian Law, at the expiration
of 6{Six} years, the money will revert to the ownership of the Nigerian
Government if nobody applies to claim the funds. Consequently, my proposal
is that I will like you as a foreigner to stand in as the next of kin to
Mr. Barry Kelly so that the fruits of this old man's labor will not get
into the hands of some corrupt government officials. This is simple;
1) I will like you to provide me immediately with your full names and
address so that the attorney will prepare the necessary documents and
affidavits, which will put you in place as the next of kin.
2) We shall employ the services of two attorneys for drafting and
notarization of the WILL and obtain the necessary documents and letter of
probate/administration in your favor for the transfer.
3) A bank account in any part of the world, which you provide, will then
facilitate the transfer of this money to you as the beneficiary/next of
kin of Mr.Barry Kelly. The money will be paid into your account for us to
share in the ratio of 75% for me and 20% for you then 5% will be set aside for any expenses that may occure during the transfer process.
There is no risk at all as all the paperwork for this transaction will be
done by the attorney and my position as the Branch Manager guarantees the
successful execution of this transaction. If you are interested, please
reply immediately via the private email address below. Upon your response,
I shall then provide you with more details and relevant documents that
will help you understand. Please observe utmost confidentiality, and rest
assured that this transaction would be most profitable for both of us
because I shall require your assistance to invest my share in your
country. Awaiting your urgent reply via email or my private telephone 234-1-7763642.
Thanks and regards
Baldwin Gozie Esq.








From daemon Fri Nov 8 20:34:26 2002



From: RCHIOVETTI-at-aol.com
Date: Fri, 8 Nov 2002 21:25:26 EST
Subject: Re: Sectioning of Lowicryl K4M embedded sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 11/08/2002 9:40:05 AM US Mountain Standard Time,
Shanling.Shi-at-unilever.com writes:

{ { Recently I freeze substitution embedded some skin samples with Lowicryl
K4M.
The blocks were cured under UV at -35C for 2 days and at room temperature for
several days. The blocks looks fine to me after UV polymerization. However,
when I section the blocks, blocks surface always get wet and the section
swell
badly on the water. It seems like sections dissolving into water. I couldn't
pick up any intact sections. I used Lowicryl HM 20 before. It worked very
well
for me. I did read the instruction of Lowicryl resin and notice that the
sectioning polar resin is different from nonpolar resin. But I could not get
good sections of Lowicyl K4M. I don't know if the blocks are not fully
polymerized or I need some special tricks to get sections. Any suggestions
are
really appreciated!

Shanling
} }

Shanling,

K4M is fairly hydrophilic, and the sections will swell if they are left on
the water for too long. You will probably have to pick up the sections
quickly after they are cut. The "dissolving" on the water surface may be a
sign of incomplete infiltration, so you might have to use a little vacuum. I
assume you did the normal stepwise infiltration in the cold, per the
instructions. Did you agitate during dehydration and infiltration?

Regarding the sectioning and wetting of the block face, here are a couple of
tips:

1. You will probably have to section the K4M with a very low water level in
the boat, much lower than you are used to, to avoid water "jumping" up onto
the black face. In fact, you can get fairly good sections when the
reflection at the knife edge is dark, rather than the normal reflection you
see when the boat is properly filled for normal sectioning.

2. If you are using a diamond knife and the edge is hydrophobic, overfill
the boat on the knife to form a positive meniscus and let the knife sit like
this for 15-30 minutes, then try to reduce the level in the boat to give a
very dark reflection along the knife edge (meaning the water level is lower
than normal). In fact, the first 2 or 3 sections may get lost in the dark
reflection so that you can barely see them, or not see them at all.

3. In the past, I have also had good luck by doing the following: After
facing and trimming the block, approach with your diamond knife with a
partially full boat and a *dry* knife edge. On the final approach, with the
microtome running and advancing, stop the microtome after the *very first*
bit of the block has been cut (usually a partial section is cut). Then
gradually bring the water level in the boat up until the water makes contact
with the dry section sitting on the knife edge.

4. At this point the water will creep under the section and lift it off the
knife. The entire knife edge will probably not be wet, but the area where
you are cutting will have water under the section and the knife edge in this
area will be wet. Start the microtome again, and gradually add water
*dropwise* between cutting cycles to slowly raise the level of water in the
boat. This will usually work with K4M, and you should be able to get
reasonably good ribbons from the block.

5. Remember, always cut with the water level lower than normal. This will
prevent the block face from getting wet.

Good luck! Let us know how things work out.

Best regards,

Bob Chiovetti


From daemon Fri Nov 8 21:40:18 2002



From: Mayandi Sivaguru :      sivagurum-at-missouri.edu (by way of
Date: Fri, 8 Nov 2002 21:33:04 -0600
Subject: Re: mercury lamp supplier

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Holly, Try labdepot.com. for bulbs in sealed containers together with
reasonable price. Tel 1-800-810-1620 or 763-557-5814

PS: Only for information purpose, no binding or whatsoever with the company.
Shiv

Mayandi Sivaguru, Ph.D.,
Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2, Tucker Hall
University of Missouri
Columbia, MO 65211-7400

Voice: 1-573-882-4895
Fax: 1-573-882-0123

www.biotech.missouri.edu/mcc/

At 09:55 AM 11/8/02 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Fri Nov 8 21:48:28 2002



From: jim shobor :      shobor-at-usa.com
Date: Fri, 08 Nov 2002 22:41:19 -0500
Subject: FOR YOUR KIND CONSIDERATION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


PLEASE TREAT AS URGENT AND CONFIDENTIAL My name is Mr Jim Shobor. I am one of these senior managers in the Bank. I work for (Zenith International Bank Nigeria PLC). And I work under the (Director of Current Account Dept.). I am contacting you presently because I need your urgent assistance in a business transaction that will be of immense benefit to both of us. I have the immediate need to claim money that have long been declared unclaimed by the chairman and some members of the board of directors of our bank. The money is the closing balance of one of our costumers. Late Eng.Temcoson Brown,am american citizen was a contractor with the feeral gov of nigeriaI I was his person account officer in the ADC plane crash of 1996 in Nigeria He supplied and installed equipment and his company creek contractors completed some of the best construction contract in the country. His closing balance in the bank,of the time of his death stood at (US$19.5M) and has been tagged unclaimed because no relative of Eng. Temcoson Brown has COMe forward to make any acclaim. All efforts to contact the relative have not been fruitful in the past four years. We have no knowledge of his next of kin. At this point. I trust you can picture what the situation is like. My colleagues and my self have made several attempts at locating person that could be remotely related to Egn. Temcoson Brown and we have been doing this for about four year (4yrs) now. I am almost alone in this enterprise as the account is forgotten entirely. I have therefore presently decided to solicit your assistance to act as the next of kin of Engr Temcoson Brown and I will be very grateful if you will be willing to help in this regard. My colleagues and I will therefore alter the original forms completed by Eng.Temcoson brown to riflect your name or any name you want us to use for the claim. We will provide you with answer to security question, which you will haveto anwser There will be no need for you to appear here in Nigeria if you follow my instructions closely. Everything should be concluded on telephone and fax between you and the bank of you participate you will be commensurately compensated. We will discuss among other primary details. I make this proposal in trust and good faith, therefore if you are interested and your agree to assist me then contract me immediately you receive this E-mail, there is a lot more to talk about. If you are not interested. Then please, do get rid of this E-mail and please forgive me if this message has upset you in anyway. Thanks you and Best Regards Mr. Jim Shobor
--
_______________________________________________
Get your free email from http://mail.usa.com



From daemon Fri Nov 8 21:54:53 2002



From: Gary Gaugler :      gary-at-gaugler.com (by way of Nestor J. Zaluzec)
Date: Fri, 8 Nov 2002 21:47:56 -0600
Subject: Fwd: TEM Negs Wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I am looking to buy TEM negs of human pathogens,
} bacteria, viruses and parasites.
}
} If you are interested in selling TEM negs in these
} categories, please contact me off-list.
}
} gary g.

gary-at-gaugler.com




From daemon Fri Nov 8 23:03:08 2002



From: paul r hazelton, PhD :      Paul_Hazelton-at-umanitoba.ca
Date: Fri, 08 Nov 2002 22:52:55 -0600
Subject: Re: Clinical turn around

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sharon-

as you can see, there are a lot of answers to turn around time. there
is an old article on staffing in the Veterans Administration Hospitals
for EM labs. i have been trying to find it in my files, but cannot.
the article states that the VA standard is (was?) 1 staff member for
every 400 samples per year. i could be slightly off on the number, it
may be 450. your 5-6 samples every other day suggests that you should
have two techs. as far as turn around, if you have sufficient staff and
the right equipment, you can give {24 hr for real rush samples, 2 days
for standard clinical, and 4-5 days for samples with special
requirements. because i need the article for a project i am involved
in, i will keep looking and send the precise number later.

now for the bonus - with the current hyper-awareness of bioterrorism,
you may get someone who wants a negative stain prep. where it is
appropriate for you to handle it you can be expected to give 5-20
minute turn around from time of arrival in the lab. it must be stressed
that you should have your local infectious diseases staff decide whether
the sample can be looked at locally or requres special treatment in a
containment facility or in Atlanta (Winnipeg if you are in Canada).
unless you are a virologist, do not assume that you, or the attending
physician can make that decision without this input. simply put, there
are some things that are simply too dangerous to handle without special
training and equipment.

paul hazelton




From daemon Sat Nov 9 10:05:58 2002



From: Jim Bentley :      bentleyj-at-ornl.gov (by way of MicroscopyListserver)
Date: Sat, 9 Nov 2002 09:55:00 -0600
Subject: MSA-RMS Scholarship Deadline Dec 10

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



MSA-RMS Travel Scholarship Award 2003

Through a cooperative agreement between the Microscopy Society of
America (MSA) and the Royal Microscopical Society (RMS), a travel
scholarship is available to a graduate student or post-doctoral
research assistant to attend a meeting or course organized by the RMS
in 2003. Details of such events can be found under events at
http://www.rms.org.uk (remember U.K. date format is day/month/year).
MSA will award up to $1000 towards travel and accommodation. RMS will
cover the registration costs and partial accommodation costs. The
applicant must be a member of MSA in good standing at the time the
application is submitted and at the time of the meeting or course.
Applications for the award should consist of:
1. Applicants full name, address, email address, and details of
academic and/or professional status;
2. A statement of up to 500 words outlining why the applicant wants
to attend a named conference or course;
3. A letter from the applicant's faculty advisor/supervisor
confirming the status of the applicant and detailing any
supplementary financial support; and
4. If required for a conference presentation, an appropriately
prepared abstract. This abstract will need to be submitted in
sufficient time and to have sufficient scientific content to be
accepted into the official program of the conference.
Applications should be sent to Dr. J. Bentley, MSA Awards Committee
Chair, Oak Ridge National Laboratory, Bethel Valley Road, PO Box
2008, Oak Ridge, TN 37831-6064 (email: bentleyj-at-ornl.gov) to arrive
no later than December 10, 2002. The awardee is expected to be
notified by December 16, 2002.

--
Jim Bentley
MSA Awards Committee Chair
Microscopy, Microanalysis, Microstructures Group
Metals and Ceramics Division
Bldg. 4515, MS-6064
Oak Ridge National Laboratory
PO Box 2008
Oak Ridge, TN 37831-6064, USA

Tel: (865)574-5067 Fax: (865)576-5413 bentleyj-at-ornl.gov
express mail use "1 Bethel Valley Rd" instead of PO Box.
** Note the new group name, building, mail-stop, fax, and area code **


From daemon Sat Nov 9 17:15:46 2002



From: David Burton :      dburton-at-nwlink.com
Date: Sat, 9 Nov 2002 14:49:24 -0800
Subject: Re: mercury lamp supplier

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Holly,

I'd suggest that if you are not getting the rated life out of your
mercury lamps that you check a couple of things. It is usually not the
fault of the lamp. If you can give me the specifics of your problem I may
be able to suggest what the cause might be. The manufacturer of the lamp
will also check and diagnose what the fault may be if you send them the
bulb.

1) Be sure that the lamp connections are clean and TIGHT! People are
tentitive installing these expensive lamps and frequently under tighten the
screws or knobs that clamp them in place. The huge thermal cycle the lamp
goes through will tend to loosen the attachments (which are also the
electrical connections) and this can shorten the life of the lamp.

2) Make sure that nobody ever turns the mercury lamp off until it has been
on a minimum of twenty minutes. Unless it is allowed to reach operating
temperture before it is turned off the life will be shortened or the lamp
will be ruined. One time can ruin a lamp.

3) Don't use the HBO 103 lamps unless you have a very new power supply that
was designed for them. Sales reps will tell you that these new longer life
lamps will work in an older system and 98% of the time they are wrong. You
will experience shorter lamp life not longer... I'd be happy to explain.

4) The lamps designed for AC power supplies have half the life of the lamps
designed for DC power supplies. This is normal.

I have purchased thousands of mercury lamps from both Bulbman and Lamp
Technologies. Both have websites. I have never found better prices
anywhere. I'd give Bulbman an A++ for customer service, slightly lower for
Lamp Technology. Both are excellent sources for all of your microscope
lightbulb needs, not just mercury lamps. Both offer discounts for orders of
more then a couple of bulbs. Quantities of 6 or 10 of the same type are
usually enough to meet the discount point. I have tried several different
manufactures of mercury lamps and found that Osram lamps perform the best
for our applications.


My only relationship with either of these companies is as a satisfied
customer.

David Burton
Optical Specialist
University of Washington



From daemon Sat Nov 9 19:33:33 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 09 Nov 2002 17:28:32 -0800
Subject: RE: I need help with digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ImageJ is OK if the file size is small.
Otherwise, you get an Out of Memory error,
even with 1.5GB of RAM.

gg

At 09:47 AM 11/8/2002, you wrote:

} Remove the long-wavelength intensity fluctuation from your images using a
} highpass Fourier Transform filter.
}
} This, and other background correction utilities are available (for free) in
} ImageJ http://rsb.info.nih.gov/ij/ ImageJ is the most versatile 2D ip&a
} software for those hwo do not require super-high speed.
}
} Cheers,
} Paul Baggethun
} Pittsburgh, PA
}
} -----Original Message-----
} } From: Smartech [mailto:smartech-at-optonline.net]
} Sent: Friday, November 08, 2002 9:04 AM
} To: To all on the list
} Subject: I need help with digital images __ How to subtract a gradaient
} from SEM image
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Nov 10 13:15:05 2002



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Mon, 11 Nov 2002 08:42:14 +1300
Subject: Re: Sectioning of Lowicryl K4M embedded sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The technique you are referring to is sometimes called "unsharp masking" and
has been used by astronomers for a long time. It is used to get better
contrast from differently illuminated areas, and it will do a background
equalization as well.

In general, you get the best results if you can take an image of the
background by itself and correct the image with that background. In a TEM
that can be done by leaving any settings undisturbed and removing the
specimen, then taking a picture of the background. I am not sure that can be
done in an SEM, though.

That leaves you with artificial background correction. Unsharp Masking is
one way to do this. Other possibilities include the calculation of a
background image from areas where the background shows through. You also
have to decide whether you want to divide or subtract. In most cases a
division is the correct operation (for example, if irregularities in a TEM
phosphor need to be taken out).

In most cases, software that deals with microscope images on a regular
basis, will have the appropriate functions built-in.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Thomas, Larry (PNNL) [mailto:Larry.Thomas-at-pnl.gov]
Sent: Friday, November 08, 2002 2:20 PM
To: Microscopy-at-Sparc5. Microscopy. Com (E-mail); 'Baggethun, Paul'


Hi Tom, and others

You have raised an interesting point that I have never really been
able to get a handle on satisfactorily, that is how do you choose the
appropriate acrylic resin to use for a new project,

eg,

should I choose LR White or LR Gold for project X, should I
chemically polymerise or perhaps use UV ?

or maybe I should try Unicryl, or Lowicryl, but which one HM20, K4M etc.

Are there others I should be considering?

I would be interested in seeing a discussion on how different people
go about choosing a particular acrylic resin for a new project they
may have to undertake.

Regards

Allan


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Sun Nov 10 13:59:48 2002



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Mon, 11 Nov 2002 08:52:03 +1300
Subject: Re: Sectioning of Lowicryl K4M embedded sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tom, and others

You have raised an interesting point that I have never really been
able to get a handle on satisfactorily, that is how do you choose the
appropriate acrylic resin to use for a new project,

eg,

should I choose LR White or LR Gold for project X, should I
chemically polymerise or perhaps use UV ?

or maybe I should try Unicryl, or Lowicryl, but which one HM20, K4M etc.

Are there others I should be considering?

I would be interested in seeing a discussion on how different people
go about choosing a particular acrylic resin for a new project they
may have to undertake.

Regards

Allan


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Sun Nov 10 14:04:19 2002



From: atgc1-at-comcast.net (by way of Ask-A-Microscopist)
Date: Sun, 10 Nov 2002 22:27:57 -0600
Subject: Ask-A-Microscopist: Blood Smear Images

Contents Retrieved from Microscopy Listserver Archives
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If anyone gets an electron to travel faster than the speed of light, please
copy me.

Peter

-----Original Message-----
} From: Vitaly Feingold [mailto:vitalylazar-at-worldnet.att.net]
Sent: Friday, November 08, 2002 9:57 AM
To: ars-at-sem.com; 'Ann-Fook Yang'; 'microscopy-at-msa.microscopy.com'


----- Original Message -----
} From: Allen Sampson {ars-at-sem.com}
To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
{yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
{Microscopy-at-sparc5.microscopy.com}
Sent: Friday, November 08, 2002 6:43 AM


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (atgc1-at-comcast.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
November 10, 2002 at 21:10:51
---------------------------------------------------------------------------

Email: atgc1-at-comcast.net
Name: Adam Georgas

Organization: UMS-Wright

Education: 9-12th Grade High School

Location: Mobile, AL

Question: Hello. I was wondering if you know were I can find a large
repository of images of blood smears.

All the best,
Adam Georgas

---------------------------------------------------------------------------


From daemon Mon Nov 11 06:35:28 2002



From: Oparowski, Joseph :      Joseph_Oparowski-at-bose.com
Date: Mon, 11 Nov 2002 07:25:15 -0500
Subject: oxygen monitor

Contents Retrieved from Microscopy Listserver Archives
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Jonathan,

We use a handheld O2 monitor system from Draeger (PAC III). It is good for
at least two years and costs under $1K with a charging stand.


Joseph

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313


-----Original Message-----
} From: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Friday, November 08, 2002 2:05 PM
To: Microscopy-at-sparc5.microscopy.com


Hi:

I am thinking I should be more careful with our liquid nitrogen handling. I
would like to monitor oxygen levels in the room we fill dewars.

So far, I have found small, battery operated low oxygen alarms for about
$300. But, they only last a year before needing replacement. The permanent
installations I have found go for over $1K.

Is this right? Any one with some sage advice? Yes, I know better safe than
sorry, but just double checking with the knowledge base.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Mon Nov 11 08:38:05 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Mon, 11 Nov 2002 08:28:30 -0600
Subject: RE: vibration?

Contents Retrieved from Microscopy Listserver Archives
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}
} If anyone gets an electron to travel faster than the speed of
} light, please
} copy me.
}
} Peter

An electron and a scanning electron spot are two very different
things. There are no lows preventing the spot from traveling with
any speed.

Vladimir


From daemon Mon Nov 11 09:20:18 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 11 Nov 2002 09:11:46 -0600
Subject: Re: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
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Dean,

I had a little sideline business for several years, running a black and white darkroom for a couple local firms. My emergency RC paper drier was a cheap Vidal Sasoon hair drier. I could dry an 8x10 print in about 30 sec to a minute. Just make sure there aren't big drops of water on the print by using a squeegee (or just a towel). Of course, this is not the greatest system for large numbers of prints, but for a reasonable quantity it's fast and cheap. And it leaves your prints so soft and manageable....

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Dean Abel [mailto:dean-abel-at-uiowa.edu]
Sent: Thursday, November 07, 2002 6:09 PM
To: Leslie Cummins
Cc: microscopy-at-msa.microscopy.com


Hello Leslie,
I use a Durst Laborator S-45 Special enlarger with a point light source
and print all my TEM and 35mm negatives on Ilford Multigrade IV RC DeLuxe
paper using Ilford Multigrade filters. It works fine for me and my bosses,
but I am sure that other papers like Kodak Polymax II RC, as suggested by
Karen Jensen, work just as well.
I learned my darkroom work using fiber-based graded papers and drying them
on a big mirrored drum, but now must I air dry my prints ever since our
dryer broke and couldn't be fixed. This is a bit of a nuisance as the
prints take overnight to dry and can't be turned in the same day they're
printed.
Does anyone have tips on drying resin coated paper quickly???
Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242-1324




From daemon Mon Nov 11 10:58:48 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Sun, 10 Nov 2002 23:48:30 -0800
Subject: Re: mercury lamp supplier

Contents Retrieved from Microscopy Listserver Archives
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On Friday, November 8, 2002, at 09:55 AM, Holly Aaron wrote:

} The last few I have gotten have not lasted the 200 hrs they should, so
} I am
} looking for a new source.
}
Dear Holly,
I second what David Burton said about turning on the lamp for short
periods; in fact I'll go further. When I had an experiment that used a
high-pressure Hg lamp, I set things up to run for 24 hours a day and
ran the experiment straight through for about three weeks. The lamp
was rated at 100 hours lifetime, and it ran for about four times that.
After the experiment, I wrapped the lamp in padding and placed it in
the back of a hood until disposal. I'm not sure whether such a
protocol can work for you, but it is definitely the case that the fewer
times one turns a lamp on and off, the longer it will last.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Mon Nov 11 12:02:51 2002



From: William J Mushock :      wim5-at-lehigh.edu
Date: Mon, 11 Nov 2002 12:53:18 -0500
Subject: RE: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You cannot generalize that the frequency of the interference on
the images is the same as that of the source.I participated in
an experiment several years ago in which we intentionally
generated an AC field at frequencies a few Hz above 60. The
resulting interference on the sem image was the difference between
the source and the 60Hz sync. In other words, a 65Hz field would look
like 5Hz(65-60). This also works for harmonics of 60Hz. A computer
monitor with a refresh rate of 75Hz would look like a 15Hz field
on an sem scan synched to 60Hz.

Also, the resonant frequency of the column suspension has to be
considered. Microscope columns usually have a resonant frequency
between 5-10Hz.




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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


My apologies to all - I made a couple of postings on this matter. The
first laid down the conditions I was using, assumptions that had to be
made
(I should have just asked the original poster, but what I wrote was
intended to be more general in application).

That original reply of mine laid out the assumption that the images were
long exposures. All later remarks I made were based on that. If,
instead,
these were short exposures then the frequencies involved could be 50 or
60
Hz. or higher. My claim that these image problems would be vibrations
was
based on those conditions.

Sorry if I caused any confusion on that point by not reiterating the
conditions in later messages..

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
ph. (630) 513-7093 fax (630) 513-7092 web http://www.sem.com


On Friday, November 08, 2002 6:57 AM, Vitaly Feingold
[SMTP:vitalylazar-at-worldnet.att.net] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} ----- Original Message -----
} } From: Allen Sampson {ars-at-sem.com}
} To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
} {yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
} {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, November 08, 2002 6:43 AM
} Subject: RE: vibration?
}
}
} } Reminds me of the very old question of can a scanning electron spot on
a
} } CRT exceed the speed of light?
} }
} } If only it could be as easy to figure as you portray. I'll try to
explain
} } the situation, but it is really more complex that I can describe in
words
} } here - and being late at night, I really won't spend much time on the
} } matter.
} }
} } Let's assume that we are working at a working distance of 20mm. For
the
} } velocity you give for electrons accelerated at 2KV (27,000 kM/S) or
15KV
} } (73,000 kM/S), that 20mm would be traversed in an exceeding small
fraction
} } of a second. However, we aren't looking at the spatial translation of
a
} } single spot. In the SEM, the spot formed on the sample is constantly
} } moving - in the case of a record image the movement of the spot is
} } generally determined by the dwell time on each of the individual pixels
on
} } each line and the number and resolution of lines in the frame.
} }
} } In the record mode, most instruments will sync the start of each line
to
} } the zero-crossing of the electrical mains. This is an intentional
attempt
} } to minimize the effect of 50 or 60 Hz. interference. Digital systems
may
} } or may not do this.
} }
} } If you go vertically down the recorded image, you are actually looking
at
} } pixels that are separated by fairly large periods of time, 1/50th or
} 1/60th
} } of a second in the case of a synched record cycle. Now let's look at
your
} } projected travel times using these figures. The difference between the
} 2KV
} } and 15KV velocities you mention is 46000 kM/S. That gives us a
difference
} } in the velocity at these two accelerating voltages of 4.35x10-9 seconds
to
} } traverse the 20mm to the sample.
} }
}
} Correct.
}
}
} } Assuming a 60 Hz system, the difference between two vertically
separated
} } pixels is 0.0167 seconds.
}
}
} That is correct for a scan speed of 60 lines per second - frame of 1000
} lines will be scanned in 16 seconds.
}
}
} } Let's say that each 1/60th of a second is split
} } into 1500 equal parts - 1000 pixels used per line and 500 portions used
} for
} } the vertical retrace. That means that each pixel would be 0.000011
} seconds
} } apart.
}
}
} Adjacent horizontal pixels will be ~ 0.00001 seconds apart. While
adjacent
} vertical pixels will be 1/60s ~ 0.016s apart.
}
}
} } Now let's look at two pixels that are on separate scan lines and
separated
} } by one additional pixel. They would be separated by a time period of
} } 0.016678 seconds (adding the pixel dwell time to the line dwell time).
}
}
} Correct.
}
}
} } Doesn't sound like much, but in EM we have to understand the effects
of
} } the orders of magnitude we work with. If you divide the above time
} } difference to traverse the working distance by the pixel time
difference
} } just mentioned, you would find that there is a 2.58x10-7 second
difference
} } between the pixels mentioned above.
}
}
} Here is the juice. When you divide a time period by a time period, the
} result
} can not be time. It can be, however, a number of events, a number of
} portions, etc. What portions? The above figure of 2.58x10-7 is the
} difference
} between the portions of a pixel, which beam will scan across
} the specimen surface, equal to the time difference between 2kV and 15kV
} electrons crossing of the WD of 20 mm. Beam deflection (scan) times
remain
} the same for both accelerating voltages, of course.
} Can you see such difference on a micrograph? The ~ 3/10,000,000 of a
pixel?
} This will be the difference attributed to accelerating voltage change
from
} 2kV to 15kV and vice versa. This is why kV change will not bring any
} noticeable change into vibration affected image.
}
} My whole point was that scan (deflection) times/speeds are compatible
with
} times/speeds of both mechanical and AC magnetic
} events. The electron velocities/timing, in contrary, are not. They are
many
} orders of
} magnitude shorter/faster than mechanical events.
}
}
} }
} } I'm tired and really don't want to extend this too much more, but
consider
} } that the vibrations seen on the images at question cover around 20 or
more
} } scan lines and figure in the velocities of a nanometer scale RMS
} vibration.
} } You'll find that there is indeed a considerable, measurable, variation
in
} } image vibrations due to accelerating voltage (as well as working
} distance).
} }
} } Amazing, isn't it?
} }
} } By the way, I still claim that the frequency of the problems in the
} } original image is much lower than 50 or 60 Hertz and is the result of
} } vibrations rather than any electro-magnetic or electronic effect.
Another
} } poster mentioned the possibility that a turbo pump may be having
problems
} } but that is also not a likely cause as the frequencies involved don't
} } match. You might, however, want to check the operation of any water
} } chiller that is in use.
}
} Perhaps so, perhaps not. It can be chiller, or turbo pump, or anything
else.
} I can't say without more information.
}
}
} }
} } If I have learned anything in working on these instruments for well
over
} } twenty years, it's that nothing is simple or straightforward. We're
} } dealing with sub-atomic particle physics here folks, an area that can
only
} } be currently described by the statistical methods of quantum theories.
} }
} } As far as I am concerned, there is no further determination to be made
-
} } the problem is one of mechanical vibrations and I would be glad to
stake
} my
} } reputation on it, as I do on any posting I make here.
} }
} }
} } Allen R. Sampson
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} }
} } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
}
} This address can not receive messages larger than 15 kb without prior
} notification.
}
}
} }
} }
} } On Thursday, November 07, 2002 10:54 PM, Vitaly Feingold
} } [SMTP:vitalylazar-at-worldnet.att.net] wrote:
} } } Hey Allen,
} } }
} } } You may found the following figures entertaining. Natural
} } } constants and results of calculations are rounded to the first
character
} } } after the decimal point, relativity effects are neglected, plain text
} } format
} } } is used. I am a bit rusty on my Physics- my apologies if I missed an
} } order
} } } or so of magnitude. Makes no difference in this example.
} } }
} } } Electron mass ~ 9.1 x 10-31kg
} } } Electron charge ~ -1.6 x 10-19 C
} } } One electronvolt energy ~ 1.6 x 10-19J
} } } Kinetic energy (of electron, or anything having mass) = mV2/2, where
m
} is
} } } the mass,
} } } V2 is the velocity square.
} } }
} } } Simple calculations yield the following velocities in kilometers per
} } second
} } } for electrons accelerated by potential differences of:
} } } 1 Volt ~ 6 x 100 km/s
} } } 200 V ~ 8.4 x 1,000 km/s
} } } 2 kV ~ 2,7 x 10,000 km/s
} } } 15 kV ~ 7.3 x 10,000 km/s
} } }
} } } Amazing, isn't it?
} } }
} } } Mechanical vibration velocity must be compatible in magnitude with
the
} } above
} } } velocities, in order for vibration effects to look different at
} different
} } } beam accelerating voltages. But SEM is made of the materials found on
} } this
} } } planet, with limited durability specifications. Thus, in order to
} vibrate
} } } with such velocities, SEM must be either made very tiny, which is not
} the
} } } case, or must disintegrate into dust, but it doesn't.
} } }
} } } Velocities of mechanical vibrations affecting SEM are in many orders
of
} } } magnitude slower
} } } than the E-beam velocities.
} } }
} } } This is why SEM stage vibration will show up exactly the same at
} } different
} } } beam accelerating voltages.
} } }
} } } Why then stray magnetic field affects an e-beam? Because electron has
} } huge
} } } charge/mass ratio, ~ 10,000,000,000. The interaction is very strong.
} } Thus,
} } } effect increases at lower accelerating voltages. Same with working
} } distance-
} } } effect increases with the distance increase. But, one needs to change
WD
} } } substantially, to see the effect for sure. That will reduce the
} } resolution,
} } } among other things. I will advice against chamber/beam/sample
geometry
} } } change during troubleshooting process.
} } }
} } } Now down to business with Ann's images. The sawtooth vertical edges
} } } appearance is the result of horizontal scan being out of phase with
the
} } } external source of either vibration, fields, or signal interference.
} Time
} } } delay between the lines at slow scan is in order of tens to hundreds
} } } milliseconds, which is within the possible vibration frequency range.
} } Same
} } } frequencies will cause wavy image at TV deflection speeds.
} } }
} } } Another point is that mechanical vibration in many cases repeats the
AC
} } line
} } } frequency, as it is caused by electric motor driven devices, so
either
} 60
} } Hz
} } } or it's harmonic(s) are present as mechanical vibrations. The only
way
} to
} } } find the problem is to troubleshoot and differentiate.
} } }
} } } I agree with other postings- more information needed to develop more
} } } accurate idea on how to proceed.
} } }
} } }
} } } Vitaly Feingold
} } } Scientific Instruments and Applications
} } } 2773 Heath Lane, Duluth GA 30096
} } } (770)232-7785 ph.
} } } (770)232-1791 fax
} } } (678)467-0012 mobile
} } }
} } } This message is made of 100% recycled electrons.
} } }
} } } This address can not receive messages larger than 15 kb without prior
} } } notification.
} } }
} } } ----- Original Message -----
} } } From: Allen Sampson {ars-at-sem.com}
} } } To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
} } } {yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
} } } {Microscopy-at-sparc5.microscopy.com}
} } } Sent: Thursday, November 07, 2002 3:50 PM
} } } Subject: RE: vibration?
} } }
} } }
} } } }
} } --------------------------------------------------------------------
----
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
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} } } }
--------------------------------------------------------------------
} } ---.
} } } }
} } } }
} } } } Vitaly,
} } } }
} } } } Most of your concerns I covered in a previous reply. However, I
have
} } to
} } } } take exception to your claim that a large change in the problem's
} } } } characteristic would result from a change in accelerating voltage
only
} } if
} } } } the problem was electro-magnetic. A reduction in the accelerating
} } voltage
} } }
} } } } will also result in a reduction of the velocity of electrons in the
} } beam.
} } } } That would cause an increased displacement of the beam as it
} traverses
} } } the
} } } } sample surface due to vibrations.
} } } }
} } } } In other words, the effects of electro-magnetic interference and
} } } vibrations
} } } } are virtually inseparable except for the frequency involved. Each
} will
} } } } affect the beam in similar ways, in regards to accelerating voltage
} and
} } } } working distance. Both will have a greater affect with a lower
} } } } accelerating voltage as well as a greater working distance.
} } } }
} } } }
} } } } Allen R. Sampson
} } } } Advanced Research Systems
} } } } 317 North 4th. Street
} } } } St. Charles, Illinois 60174
} } } }
} } } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
} } } }
} } } }
} } } } On Wednesday, November 06, 2002 8:58 PM, Vitaly Feingold
} } } } [SMTP:vitalylazar-at-worldnet.att.net] wrote:
} } } } } Could be vibration. Could also be magnetic field. And, could be
} } signal
} } } } } interference, such as ground loop. What type of SEM are you
using?
} } } } }
} } } } } I will omit remedies for the vibration problem, since Allen
Sampson
} } did
} } } } } pretty good job addressing that.
} } } } }
} } } } } Stray AC line frequency magnetic field: try acquiring image at
} } } different
} } } } } accelerating voltages. It is very important that all other
geometry
} } } } } parameters will remain the same: particular area of a particular
} } } } specimen,
} } } } } WD, tilt, magnification, spot size. Contrast, brightness, focus,
and
} } } } } stigmator settings can be changed. If the problem is much worse
at,
} } say,
} } } } 2
} } } } } kV, than at, say, 15kV, you are dealing with magnetic field. You
may
} } } also
} } } } } try to keep 3 axes AC milliGauss meter at the SEM, and notice
what
} it
} } } } } measures, but catching stray magnetic interference this way may
be a
} } } } little
} } } } } tricky. These meters, though, are very inexpensive, and available
} } from
} } } } many
} } } } } test instruments suppliers.
} } } } }
} } } } } Now we are getting down to statistically most likely problem-
signal
} } } } } interference and ground loops. It is difficult to go any further
} } without
} } }
} } } } } knowing the SEM type, and acquisition system (if separate) type.
Too
} } } many
} } } } } possibilities exist, but the very first- does your acquisition
} } software
} } } } } have an option somewhere in the settings which reads something
like
} } "60
} } } } } Hertz sync", or "AC line synchronization", or "Line sync", etc.,
} } etc.?
} } } If
} } } } } yes, check (or uncheck) that option and see what happens. Further
} } } } remedies
} } } } } of this problem will include eliminating ground loops. Example-
sh
} } ielded
} } } } } cable(s) has shield connected on both sides, and shield is used
as
} } one
} } } of
} } } } } the signal wires. This is frequently done, and does jeopardize
} } } instrument
} } } } } interference tolerance. Another example- one of the accessories
is
} } } } } susceptible to some kind of interference, and must be powered
} through
} } } the
} } } } } isolation transformer. The latter remedy will only work with
decent
} } } } (better
} } } } } if dedicated) ground. The fact that the problem is not present
all
} } the
} } } } time
} } } } } does not mean that everything is perfect inside the SEM. Besides,
} } going
} } } } } after the EM interference source could be inefficient and
} } frustrating,
} } } if
} } } } } not impossible. Improving grounding/shielding/power connection
might
} } be
} } } } } easier.
} } } } }
} } } } }
} } } } } Vitaly Feingold
} } } } } Scientific Instruments and Applications
} } } } } 2773 Heath Lane, Duluth GA 30096
} } } } } (770)232-7785 ph.
} } } } } (770)232-1791 fax
} } } } } (678)467-0012 mobile
} } } } }
} } } } } This message is made of 100% recycled electrons.
} } } } }
} } } } } This address can not receive messages larger than 15 kb without
} prior
} } } } } notification.
} } } } }
} } } } } ----- Original Message -----
} } } } } } From: Ann-Fook Yang {yanga-at-agr.gc.ca}
} } } } } To: {microscopy-at-sparc5.microscopy.com}
} } } } } Sent: Wednesday, November 06, 2002 2:45 PM
} } } } } Subject: vibration?
} } } } }
} } } } }
} } } } } }
} } } }
} }
------------------------------------------------------------------------
} } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
of
} } } } America
} } } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } }
} } } }
} }
-----------------------------------------------------------------------.
} } } } } }
} } } } } }
} } } } } } Hi everyone,
} } } } } }
} } } } } } I have two SEM micrographs on the web:
} } } } } } http://www.magma.ca/~scimat/Defect.htm
} } } } } } The micrographs show zagged edges taken at 20 kX. Such
phenomenon
} } } does
} } } } } not present all the time. Can anyone suggest what causes the
problem
} } and
} } } } how
} } } } } to solve it?
} } } } } } Thanking in advance.
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } } AnnFook Yang
} } } } } } EM Unit,
} } } } } } Eastern Cereal and Oilseed Research Centre,
} } } } } } Room 2091, Bldg. 20,
} } } } } } Central Experimental Farm,
} } } } } } Ottawa, Ontario
} } } } } } Canada K1A 0C6
} } } } } }
} } } } } } Tel: 1-613-759-1638
} } } } } } Fax: 1-613-759-1701
} } } } } }
} } } } } } e-mail: yanga-at-em.agr.ca
} } } } } }
} } } } } }
} } } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } }
} } } }
} } }
} } }
} } }
} }
}
}
}
}
}
}
}


From daemon Mon Nov 11 12:18:47 2002



From: Hong Yi :      hyi-at-emory.edu
Date: Mon, 11 Nov 2002 13:08:49 -0500
Subject: EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thank everyone who replied to my posting on EM Atlas. For those who
requested forwarding of the replies, here is the list;


"Histology: A text and atlas" by Johannes A G Rhodin. 1974, Oxford
University Press.

“Cell and Tissue Ultrastructure: A Functional Perspective” by Pat Cross
and K. Lynn Mercer (1993, ISBN 0-7167-7033-4 )

"The Cell", by Dr. Don Fawcett, 2nd edition, 1981, Saunders,
Philadelphia, ISBN: 0-7216-3584-9.

“Cell Structure; an Introduction to Biomedical Electron Microscopy”,
3rd edition, 1982, Churchill Livingstone ISBN 0 443 02324 7,

"Particle Atlas" produced by the McCrone Institute.

“Histology” by Rhodin, (1974, Library of Congress Catalogue No.73-90374)

“BIOMEDICAL ELECTRON MICROSCOPY” by A.V. Maunsbach and B.A. Afzelius,
Academic Press 1999.

“The Fine Structure of Nervous System” Peter Davis and Sanford Paley,
3rd edition, 1991, Oxford

"Ultrastructural Pathology of the Cell and Matrix" by Ghadially


On the list, the first two were recommended most frequently. I
purchased a used copy of “Cell and Tissue Ultrastructure: A Functional
Perspective” from Amazon.com for $18.00.

Hong



From daemon Mon Nov 11 13:08:22 2002



From: MicroscopyToday :      microtod-at-optonline.net
Date: Mon, 11 Nov 2002 13:57:12 -0500
Subject: Re: Sectioning of Lowicryl K4M embedded sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Alan,

You've made a great point that would be of considerable interest to many
microscopists.

I would love to see this discussion ensue and then a final wrap-up
written for publication in Microscopy Today!

Ron Anderson, Editor
Microscopy Today

-----Original Message-----
} From: Allan Mitchell [mailto:allan.mitchell-at-stonebow.otago.ac.nz]
Sent: Sunday, November 10, 2002 2:42 PM
To: Microscopy-at-sparc5.microscopy.com
Cc: phillipst-at-missouri.edu


Hi Tom, and others

You have raised an interesting point that I have never really been
able to get a handle on satisfactorily, that is how do you choose the
appropriate acrylic resin to use for a new project,

eg,

should I choose LR White or LR Gold for project X, should I
chemically polymerise or perhaps use UV ?

or maybe I should try Unicryl, or Lowicryl, but which one HM20, K4M etc.

Are there others I should be considering?

I would be interested in seeing a discussion on how different people
go about choosing a particular acrylic resin for a new project they
may have to undertake.

Regards

Allan


} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

From daemon Mon Nov 11 13:30:00 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 11 Nov 2002 12:16:29 -0700
Subject: RE: vibration?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yep, as long as you can't transmit any information between the spot
positions faster than light, you can WARP speed on them.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Dusevich, Vladimir [mailto:dusevichv-at-umkc.edu]
Sent: Monday, November 11, 2002 7:29 AM
To: microscopy-at-msa.microscopy.com



}
} If anyone gets an electron to travel faster than the speed of
} light, please
} copy me.
}
} Peter

An electron and a scanning electron spot are two very different
things. There are no lows preventing the spot from traveling with
any speed.

Vladimir


From daemon Mon Nov 11 14:14:52 2002



From: Bruce Brinson :      brinson-at-rice.edu
Date: Mon, 11 Nov 2002 14:06:37 -0800
Subject: TEM GaAs cross section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.



From daemon Mon Nov 11 14:27:45 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 11 Nov 2002 15:20:11 -0500
Subject: Re: TEM printing-polycontrast paper?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The best I ever used was manufactured by Ilford for their RC multigrade
paper. It took up about 40" on a standard bench top, but it processed an
8x10 in about 30 seconds and REALLY created a glossy result. Here's a site
that advertises one with a price around $3k (WOW!)

http://www.bhphotovideo.com/product/24574/IL1250/REG/776

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone: 610-738-0437
eMail: fmonson-at-wcupa.edu


-----Original Message-----
} From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu]
Sent: Friday, November 08, 2002 9:39 AM
To: Dean Abel
Cc: Leslie Cummins; microscopy-at-msa.microscopy.com


Print dryers for resin coated papers are available, of course right now I
can't
remember where! Try a google search for print dryers.

Geoff

Dean Abel wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Leslie,
} I use a Durst Laborator S-45 Special enlarger with a point light
source
} and print all my TEM and 35mm negatives on Ilford Multigrade IV RC DeLuxe
} paper using Ilford Multigrade filters. It works fine for me and my
bosses,
} but I am sure that other papers like Kodak Polymax II RC, as suggested by
} Karen Jensen, work just as well.
} I learned my darkroom work using fiber-based graded papers and
drying them
} on a big mirrored drum, but now must I air dry my prints ever since our
} dryer broke and couldn't be fixed. This is a bit of a nuisance as the
} prints take overnight to dry and can't be turned in the same day they're
} printed.
} Does anyone have tips on drying resin coated paper quickly???
} Dean Abel
} Biological Sciences 138BB
} University of Iowa
} Iowa City IA 52242-1324

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Mon Nov 11 14:45:10 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Mon, 11 Nov 2002 16:43:04 -0500
Subject: TEM Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Very good point. It absolutely could be a beat frequency. Either mechanical,
magnetic, or signal interference.

Ann, please do not be discouraged by all seeming complexity. Practical
solution could be simpler than bulletproof-correct description covering
all possible aspects, causes, and manifestations of the problem.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: William J Mushock {wim5-at-Lehigh.EDU}
To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
{yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
{Microscopy-at-sparc5.microscopy.com}
Sent: Monday, November 11, 2002 12:53 PM


Dear Listers:

We are planning to upgrade our TEM digital camera purchased 9 years ago
from. We do alot of kidney biopsies as well as various research specimens.
Does anyone have any experience with the higher resolution TEM digital
cameras? We are looking for something around 2-4 megapixels.

Thanks

Karen

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954



From daemon Mon Nov 11 16:49:22 2002



From: MicroscopyToday :      microtod-at-optonline.net
Date: Mon, 11 Nov 2002 17:39:10 -0500
Subject: Microscopy Today November/December Issue TofC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Colleagues,

Here is the Table of Contents for the November/December issue
of "Microscopy Today" Magazine. A publication of the
Microscopy Society of America.

I will close the subscription list for this issue on
November 13th. Microscopists in the USA may obtain free
subscriptions via the form at www.microscopy-today.com
Non-USA members anywhere in the world also receive MT free.
Non-USA, non-MSA members are charged a fee to cover postage.
Details on the web site.

This issue will mail with the Call for Papers for the 2003
M&M meeting in San Antonio to over 23,000 people, derived from
the combined MSA, MAS, IMS, and CIASEM membership as well
as the 11,500 regular subscribers to Microscopy Today.

Ron Anderson, Editor
Microscopy Today

TABLE OF CONTENTS
When Dinosaurs Became Extinct, What Happened To The Insects?
Stephen W. Carmichael, Mayo Clinic

A Comparison Of Grain Size Measurements In Al-Cu Thin Films:
Imaging Vs. Diffraction Techniques5
L.M. Gignac,* C.E. Murray,* K.P. Rodbell,* M. Gribelyuk+

IBM T.J. Watson Research Center, IBM Microelectronics Division
Using a Sony Cyber-Shot Digital Camera for Photomicrography
Gregor Overney, Agilent Technologies Inc.

Use Adobe Acrobat to Keep Original Resolutions and to Make
TIFF Files From Any Program
Jerry Sedgewick, University of Minnesota

Confocal Microscopy System Performance: Laser
Power Measurements
Robert M. Zucker, PhD, U.S. Environmental Protection Agency

Imaging of Shallow Surface Topography by the Low-Loss
Electron (LLE) Method in the Scanning Electron Microscope
Oliver C. Wells, IBM Research Division

New and Interesting at Microscopy & Micranalysis-2002

Penetration Rates of Formaldehyde
Bryan R. Hewlett, McMaster University Medical Centre

Digital Imaging in K-12 Biology
James Ekstrom, Phillips Exeter Academy

Designing A Microscopy/analytical Instrumentation Facility:
Step By Step Procedure
Judy A. Murphy, San Joaquin Delta College

Preparing Ultra-Smooth SEM Stud Surfaces
Dr. Carole Hickman, University of California, Berkeley

Protection from Sulfur Hexafluoride Leaks
Mick Thomas, Cornell University

Industry News

Index of Advertisers




From daemon Mon Nov 11 18:52:53 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 11 Nov 2002 17:35:59 -0700
Subject: TEM GaAs cross section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bruce,

try this one. I used to do this on a regular basis when I was still doing
TEM. We looked at anything from heterostructures to dislocations, and had a
good success with this technique:

1) buy some 3mm OD steel pipe (or perhaps other strong material)

2) cut rings with a thickness of half a mm to a mm off the pipe. Clean rings
thorougly and remove burrs.

3) with the rest of the pipe, drill a ring shaped groove into the material
you are interested in around the part you want to prepare.

4) Glue with a strong epoxy one of the rings into the groove.

5) grind sample from backside until you have a freestanding steel ring with
the GaAs or other material inside.

6) Dimple

7) Ion mill

8) Enjoy.

the steel ring gives it enough support for handling with tweezers. You
probably need to experiment with the thickness of the rings and other
parameters, but it works, once you got everything under control.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Monday, November 11, 2002 3:07 PM
To: MSA Listserver


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.



From daemon Mon Nov 11 19:21:31 2002



From: Lenox Laser Lab :      lenox-at-lenoxlaser.com (by way of
Date: Mon, 11 Nov 2002 19:17:26 -0600
Subject: Microscope Calibration Measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Do you happen to know of any NIST traceable lab that can certify optical
apertures?

Much thanks for any help,

Doug Janssen


From daemon Mon Nov 11 20:06:34 2002



From: Tom Pella :      tom_pella-at-tedpella.com
Date: Mon, 11 Nov 2002 17:58:43 -0800
Subject: Clinical turn around

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A paper was presented in August at the Ultrapath meeting and will be
published in Ultrastructural Pathology where diagnostic EM turnaround time
was reduced to 4 hours. It involved digital image processing and storage
within patient files and high-speed tissue specimen processing. Such an
approach might help you meet your workload. I believe Ron Austin of LSU and
Jon Charlesworth of Mayo Clinic, two of the authors on the paper, as well as
others might be able to answer questions about this.

Tom Pella

-----Original Message-----
} From: Sharon Drew (by way of MicroscopyListserver)
[mailto:drewsh-at-musc.edu]
Sent: Wednesday, November 06, 2002 2:27 PM
To: Microscopy-at-sparc5.microscopy.com


Hi!
I run a diagnostic TEM laboratory in SC.
One tech.
6 to 5 samples every other day at least.
What is the turn around time asked for your labs out there that are
also doing diagnostic/clinical work and where does that number come
from?
Thank you for any help.
My supervisor is saying 2 days but I think that is close to impossible
when only one tech doing everything including transport of tissue from
another lab and scoping all case that come in.
Sharon Drew
Diagnostic Pathology EM
Charleston, SC



From daemon Mon Nov 11 21:14:56 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 11 Nov 2002 21:02:46 -0600
Subject: Eighth International Live-cell Course.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eighth Annual INTERNATIONAL 12-Day Short Course on

3D Microscopy of Living Cells
June 15 - 26, 2003 (Pre-course: June 14)


Seventh, Post-course Workshop on

3D Image Processing,
June 28 - July 30, 2003



Organized by Prof. James Pawley,
(University of Wisconsin-Madison)

in association with

The Departments of Pharmacology and Physiology
and the Brain Research Centre,
University of British Columbia
Vancouver, BC, Canada


DATES

Applications must be received by March 15, 2003
Deposit due April 15, 2003
Registration 5:00 - 7:00 PM Sunday, June 9, 2003
First Lecture 7:30 PM Sunday, June 9, 2003
Live-cell Course ends, noon Thursday, June 20, 2003
3D Image Processing Course, June 22 - 24, 2003


APPLICATIONS DUE BY MARCH 15, 2002


APPLICATIONS
Applicants must complete a questionnaire on the web to assess
knowledge level, field of interest and proposed personal, live-cell,
project. Enrollment will be limited to about 24 participants (exact
number depends on number of 3D Systems available). Selection will be
made on the basis of background and perceived need. Those without
previous LM experience will be provided with access to basic texts to
read before the course begins. Application forms requesting
information on field of interest and level of experience may be
down-loaded from the WWW site at
http://www.3dcourse.ubc.ca/application.htm , or obtained from:

Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU

Additional information is available from:
http://www.3dcourse.ubc.ca/brochure.htm

We expect to have at least 11, 3D microscope workstations for student
use and there will be an international faculty of 20.

Application deadlines:

Application forms must be received for screening by March 1, 2003.
Successful applicants will be notified by April 1, and a deposit of
50% must be received by April 15, 2003. In general, refunds of the
deposit will only be possible if someone on the waiting list can take
the place. The remainder is due before Registration.


Pre-Course Tuition (1/2 day Basic Optical principles) $120 (US)
3D Live-cell Course Tuition (includes lunches and snacks): $2450 (US)
Workshop Tuition (includes lunches and snacks): $900 (US)

Room/board about $40/day (US) depending on room type.

I hope that this includes all of the information that you need, but
if not, please get back to me.
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
"A scientist is not one who can answer questions but one who can
question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Mon Nov 11 21:15:08 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 11 Nov 2002 22:08:55 -0500
Subject: TEM GaAs cross section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I highly recommend the small angle cleavage technique for III-V materials. It is a very easy technique to learn, produces superior samples to any other technique, and it is very quick, approx. 9 samples per hour. Out of that 9 samples, about 5-7 will be usable and 2-3 of those will be fantastic. Go to the South Bay Technology web page and check out their microcleave kit. There are some examples of a MQW GaAs-InGaAs structure that I prepared in their document section along with some other examples. Send me your address, and I can send you a disk with a detailed poster on how to do it. You can also go to the #4 TEM sample prep book from MRS proceedings. John McCaffrey and I have a detailed discussion on how to do it.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Monday, November 11, 2002 5:07 PM
To: MSA Listserver


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.



From daemon Tue Nov 12 04:21:34 2002



From: Erasmus, Willem (WJ) :      willem.erasmus-at-sasol.com
Date: Tue, 12 Nov 2002 12:07:12 +0200
Subject: SEM particle size measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear microscopists

I would like to estimate the particle size distribution of spherical particles that were embedded in a continuous solid phase and polished to obtain a cross-section. The cross-sectioning could have cut the particles at any point, which means that the size observed is probably not the size of the original particles.Is it possible to deduce the particle size distribution from measurements of the cross-sectional areas ? Can anyone suggest a good reference that may explain how this estimation is accomplished ?

Regards
W Erasmus

Willem Erasmus
Snr. Scientist, Materials Characterization Group
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]



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From daemon Tue Nov 12 06:22:52 2002



From: DrJohnRuss-at-aol.com
Date: Tue, 12 Nov 2002 08:03:03 EST
Subject: Re: SEM particle size measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pat,

We have a small exhaust equipped gas storage room where we fill LN2 dewars.
We keep the doors in the area open when we fill. If the flow rate is
moderate, we do not have a problem. However, if we get an occasional med
pressure dewar, the alarm will sound. The sensor is about 5 feet off the
floor. Hope this helps.


Joseph

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313


-----Original Message-----
} From: psconnel-at-sas.upenn.edu [mailto:psconnel-at-sas.upenn.edu]
Sent: Monday, November 11, 2002 7:53 PM
To: Oparowski, Joseph
Cc: Microscopy-at-sparc5.microscopy.com



In a message dated 11/12/02 5:42:19 AM, willem.erasmus-at-sasol.com writes:

} I would like to estimate the particle size distribution of spherical
particles
} that were embedded in a continuous solid phase and polished to obtain a
} cross-section. The cross-sectioning could have cut the particles at any
} point, which means that the size observed is probably not the size of the
} original particles.Is it possible to deduce the particle size distribution
} from measurements of the cross-sectional areas ? Can anyone suggest a good
} reference that may explain how this estimation is accomplished ?
}
}
}
} Regards
}
} W Erasmus

You can find the explanation and math in Practical Stereology, 2nd edition,
J. C. Russ & R. T. Dehoff, Plenum Press, 2001. Assuming that you have a way
to measure the intersection sizes, the calculations can be done in a
spreadsheet. However be warned that the technique, which has been known and
used since the 1920's, has two problems. First, it is critically dependent on
the assumption that the shape of the features is exactly known and that it
remains the same for large and small features. Second, it is mathematically
unstable, with the precision of the 3D data being much poorer than that of
the 2D intersections. These problems have caused many stereologists to prefer
another approach that provides the mean and standard deviation of the
distribution without making any shape assumptions. that method is also
described in the book. Original references to important papers are provided
for all of the stereological techniques.

John Russ


From daemon Tue Nov 12 09:25:47 2002



From: evgenia.pekarskaya-at-exxonmobil.com
Date: Tue, 12 Nov 2002 10:15:04 -0500
Subject: Re: SEM particle size measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Willem,

A good source of information is:
"Metallography. Principles and Practice" by George F. Vander Voort.

Regards,

Evgenia

**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355




"Erasmus, Willem
(WJ)" To: {Microscopy-at-sparc5.microscopy.com}
{willem.erasmus-at-sa cc:
sol.com} Subject: SEM particle size measurement


11/12/02 05:07 AM





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The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Dear microscopists

I would like to estimate the particle size distribution of spherical
particles that were embedded in a continuous solid phase and polished to
obtain a cross-section. The cross-sectioning could have cut the particles
at any point, which means that the size observed is probably not the size
of the original particles.Is it possible to deduce the particle size
distribution from measurements of the cross-sectional areas ? Can anyone
suggest a good reference that may explain how this estimation is
accomplished ?

Regards
W Erasmus

Willem Erasmus
Snr. Scientist, Materials Characterization Group
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]



+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

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From daemon Tue Nov 12 10:32:38 2002



From: R. Ann Bliss :      bliss5-at-llnl.gov
Date: Tue, 12 Nov 2002 08:23:04 -0800
Subject: RE: TEM GaAs cross section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bruce;

Mike Bode's reply reminds me of the method my colleague uses for
cross sections. He first sandwiches the sample material between
pieces of silicon with G1 epoxy (Gatan) in a vise til the epoxy sets
up.Then the sandwich is waxed into a holder for a sonic cutter
(again, Gatan). The material cored out is epoxied into a brass tube
with a 3mm outside diameter.This tube is placed on a saw with a 3"
diameter diamond blade and several disks are cut. Now go to the lap,
dimple and ion mill steps.

I have no connection with Gatan; we happily use their products.

--

+++++++++++++++++++++++++++++

R. Ann Bliss
Technician, Chemistry and Materials Science
Materials Science and Technology Division
Lawrence Livermore National Laboratory

_____________________________


From daemon Tue Nov 12 11:17:09 2002



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Tue, 12 Nov 2002 11:09:22 -0600
Subject: Osmication - R-OTO technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have just read Willingham & Rutherford (1984) J. Histochem Cytochm
32(4):455-460 paper on the use of ferrocyanide reduced osmium with the
osmium-thiocarbohydrazide-osmium (OTO) technique of Seligman. I thought
the conclusion that R-OTO gave a superior preservation and contrast of
membranes in TEM was a good one based on their images. But when I did a
Medline search for papers using either the OTO or R-OTO technique, the
limited number of papers was essentially limited to SEM work. Perhaps the
method is buried in papers and not coming up in a keyword search. Or maybe
no one uses it or there are problems with it?? Has any one used the R-OTO
(or even OTO) technique with tissue biopsies (Willingham & Rutherford use
cell cultures so it is tough to extrapolate the correct osmication
times)? Are there hidden pitfalls and disadvantages to this
technique? Any comments welcome. Tom


Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From daemon Tue Nov 12 11:22:13 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 12 Nov 2002 09:15:06 -0800
Subject: TEM GaAs cross section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bruce;
I don't recommend using steel rings as supports, as these will couple to the filed of the microscope lens and interfere with imaging. You can get brass tube from Gatan (or from a hobby shop for a lot less). Also, get vacuum tweezers if you don't want to fracture your samples. However, you will have a long way to go even after that point. 3-5 compunds containing indium and antimony dissociate during normal ion milling. You will need to consult a series of papers written by Tony Cullis during the late '80s, published in Ultramicroscopy, on the low temperature and iodine techniques necessary to avoid a mess.

John Mardinly
Intel



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Monday, November 11, 2002 2:07 PM
To: MSA Listserver


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.



From daemon Tue Nov 12 12:57:55 2002



From: Marek Malecki, M.D., Ph.D., Professor :      MMalecki-at-wisc.edu
Date: Tue, 12 Nov 2002 12:52:40 -0800
Subject: RE: Clinical turn around

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sharon and Tom:
In fact, Prof. Johanessen at Univ. of Bergen and later at Oslo published 4h
protocols (from biopsy taking to prints on the table) in the early 80s and
summarized in his basic book on ultrastructural pathology.
Cheers,
Marek.


At 05:58 PM 11/11/02 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Nov 12 13:28:35 2002



From: jerzy.gazda-at-amd.com
Date: Tue, 12 Nov 2002 13:20:16 -0600
Subject: SEM particle size measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Willem,
A great start will be the "Stereology" and "The Image Processing Handbook" by John Russ. Check out Amazon.com or B&N.com. The stereological formula and examples are given there. Probably any other stereology text would also have the examples.

Have Fun,

Jerzy
******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************


-----Original Message-----
} From: Erasmus, Willem (WJ) [mailto:willem.erasmus-at-sasol.com]
Sent: Tuesday, November 12, 2002 4:07 AM
To: Microscopy-at-sparc5.microscopy.com



Dear microscopists

I would like to estimate the particle size distribution of spherical particles that were embedded in a continuous solid phase and polished to obtain a cross-section. The cross-sectioning could have cut the particles at any point, which means that the size observed is probably not the size of the original particles.Is it possible to deduce the particle size distribution from measurements of the cross-sectional areas ? Can anyone suggest a good reference that may explain how this estimation is accomplished ?

Regards
W Erasmus

Willem Erasmus
Snr. Scientist, Materials Characterization Group
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]



+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

NOTICE: Please note that this eMail, and the contents thereof,
is subject to the standard Sasol eMail disclaimer which may be found at:
http://www.sasol.com/disclaimer.htm

If you cannot access the disclaimer through the URL attached and
you wish to receive a copy thereof please send an eMail to
disclaimer-at-sasol.com. You will receive the disclaimer by return eMail.





From daemon Tue Nov 12 13:39:33 2002



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Tue, 12 Nov 2002 14:56:55 -0600
Subject: Date Correction Eighth International Live-cell Course. SORRY.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bruce,

You might want to try the high angle polishing technique described in “The
Concept of High Angle Wedge Polishing and Thickness Monitoring in TEM Sample
Preparation,” Hao Li, and L. Salamanca-Riba, Ultramicroscopy 88, 171-8
(2001). With this technique you can make samples that have electron
transparent areas without any ion milling. Because of the high angle
polishing the samples are not very thin every where and, therefore, are less
likely to break.

I advise you to try the technique first with Si as it is a lot easier to
make a TEM sample of Si than InSb. When you try the InSb sample you should
use diamond paper with very fine particle size (3 microns) very early in the
polishing process because InSb is a very soft material. Then change to finer
particle size sooner than you would do with Si so that you can control
better how much material you are removing.

With this technique we were able to prepare cross sections of AlSb films
grown on GaSb substrates.

Good luck,

Lourdes Salamanca-Riba

----- Original Message -----
} From: "Bruce Brinson" {brinson-at-rice.edu}
To: "MSA Listserver" {microscopy-at-sparc5.microscopy.com}
Sent: Monday, November 11, 2002 5:06 PM


MURPHY STRIKES AGAIN!

It never fails, I make the announcement and the Course Site goes down
(it will be back up soon!). Then several of you point out
inconsistencies in the dates in the Announcement. The dates have been
fixed below (thanks to Iain Johnson,at Molecular Probes!!) and the
Site will soon be repaired.

Thank you for your patience!

Jim P.


Eighth Annual INTERNATIONAL 12-Day Short Course on

3D Microscopy of Living Cells
June 15 - 26, 2003 (Pre-course: afternoon, June 14)


Seventh, Post-course Workshop on

3D Image Processing,
June 28 - July 30, 2003



Organized by Prof. James Pawley,
(University of Wisconsin-Madison)

in association with

The Departments of Pharmacology and Physiology
and the Brain Research Centre,
University of British Columbia
Vancouver, BC, Canada


DATES

Applications must be received by March 15, 2003
Deposit due April 15, 2003
Registration 5:00 - 7:00 PM Saturday, June 14, 2003
First Lecture 7:30 PM Saturday, June 14, 2003
Live-cell Course ends, noon Thursday, June 26, 2003
3D Image Processing Course, June 28 - 30, 2003


APPLICATIONS DUE BY MARCH 15, 2003


APPLICATIONS
Applicants must complete a questionnaire on the web to assess
knowledge level, field of interest and proposed personal, live-cell,
project. Enrollment will be limited to about 24 - 32 participants
(exact number depends on number of 3D Systems available). Selection
will be made on the basis of background and perceived need. Those
without previous LM experience will be provided with access to basic
texts to read before the course begins. Application forms requesting
information on field of interest and level of experience may be
down-loaded from the WWW site at
http://www.3dcourse.ubc.ca/application.htm , or obtained from:

Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU

Additional information is available from:
http://www.3dcourse.ubc.ca/brochure.htm

We expect to have at least 11, 3D microscope workstations for student
use and there will be an international faculty of 20.

Application deadlines:

Application forms must be received for screening by March 15, 2003.
Successful applicants will be notified by April 1, and a deposit of
50% must be received by April 15, 2003. In general, refunds of the
deposit will only be possible if someone on the waiting list can take
the place. The remainder is due before Registration.


Pre-Course Tuition (1/2 day Basic Optical principles) $120 (US)
3D Live-cell Course Tuition (includes lunches and snacks): $2450 (US)
Workshop Tuition (includes lunches and snacks): $900 (US)

Room/board about $40/day (US) depending on room type.

I hope that this includes all of the information that you need, but
if not, please get back to me.
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
"A scientist is not one who can answer questions but one who can
question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Tue Nov 12 15:56:11 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 12 Nov 2002 14:38:55 -0700
Subject: TEM GaAs cross section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John is of course correct on both points: the steel could potentially
interfere with the magnetic field of the lenses and create unwanted effects.
I thought we were using steel (it's been a while), the material was
definitely not copper, but perhaps it was some other non-magnetic metal.

For the milling I always used LN2 cooling as John suggests, and I also used
Iodine. It helps to keep the milling as short as possible, so a careful
dimpling is important.

Thanks, John, for pointing that out.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Tuesday, November 12, 2002 10:15 AM
To: Bruce Brinson; MSA Listserver


Bruce;
I don't recommend using steel rings as supports, as these will
couple to the filed of the microscope lens and interfere with imaging. You
can get brass tube from Gatan (or from a hobby shop for a lot less). Also,
get vacuum tweezers if you don't want to fracture your samples. However, you
will have a long way to go even after that point. 3-5 compunds containing
indium and antimony dissociate during normal ion milling. You will need to
consult a series of papers written by Tony Cullis during the late '80s,
published in Ultramicroscopy, on the low temperature and iodine techniques
necessary to avoid a mess.

John Mardinly
Intel



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Monday, November 11, 2002 2:07 PM
To: MSA Listserver


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.



From daemon Tue Nov 12 16:58:36 2002



From: Shu-You Li :      syli-at-northwestern.edu
Date: Tue, 12 Nov 2002 16:49:11 -0600
Subject: Re: TEM GaAs cross section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bruce,

While previous replies focused on grid-free technique, I would like to
comment on the way you did out there.

As you already realized, the dimpled sample is very hard to handle. This
is reasonable because the center of the dimpled area is down to a couple
of microns! Accordingly what I normally do is glue the sample onto
copper grid BEFORE removing it from the support glass.
1. dimple on a glass post.
2. apply very small amount of glue (M-bond or G1 epoxy).
3. put copper grid on the top of the sample.
4. wait for glue curing. Heat up if necessary.
5. put the post in acetone.

Because M-bond or G1 epoxy can not be removed by acetone, you will have
your sample stands together with the copper grid.

if your sample is somewhat thick (more than 70microns), you can also glue
copper grid onto the sample before dimpling. Routinely I use both method
and the yield is always ~1.

Hope this helps,
Shuyou.


On Mon, 11 Nov 2002 14:06:37 -0800
Bruce Brinson {brinson-at-rice.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello TEM Microscopist and sample prep masters,
}
} I've spent a humbling amount of time trying to learn to prepare TEM
} cross section samples from GaAs substrates. I should also point out that
} I do not have mentor for this project. For the moment I am working with
} dummy samples. When I start working with the real material of interest
} (InSb AlAs quantum wells), we will typically have enough material for
} one attempt... so I need to get the yield (to the TEM) up to or atleast
} close to 1.
} I am able to block, polish and dimple the material easily enough but
} loose many samples transferring from the dimpling block (glass post) to
} the Cu support.
}
} My procedure is .... then
} 1. dimple on a glass post.
} 2. I then put a piece of filter paper and stack 2 u-scope slides in a
} dish. I put the post, sample down, with one edge of the post on the
} u-scope slides allowing a space for the dimpled bit to fall off then
} immerse in acetone .
} To this point my yield is ~1
}
} Mounting to the Cu support is where I often loose the samples due to
} fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
} grids and all the caution I can muster. I lift the sample out and
} attempt to lay it on a glue bearing support. Some times I get there in 1
} piece, sometimes I don't.
}
} Since we need a yield of 1 for 30+ samples this is a real problem.
}
} Any advice on the sample prep. of this material would be greatly
} appreciated.
}
}
} Bruce Brinson
} Rice U.

_____________________________
Shu-You Li, Ph.D.
Electron Microscopist
Electron Probe Instrumentation Center(EPIC)
Northwestern University
2220 Campus Drive, 1141 Cook Hall
Evanston, IL 60208, USA
Ph: (847) 491-7798, Fax: (847) 491-7820
Email: syli-at-northwestern.edu; syli16-at-hotmail.com
http://pubweb.northwestern.edu/~sli974





From daemon Tue Nov 12 17:23:51 2002



From: RCHIOVETTI-at-aol.com
Date: Tue, 12 Nov 2002 18:12:06 EST
Subject: Re: Microscope Calibration Measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 11/11/2002 6:27:20 PM US Mountain Standard Time,
lenox-at-lenoxlaser.com writes:

{ { Do you happen to know of any NIST traceable lab that can certify optical
apertures?

Much thanks for any help,

Doug Janssen
} }

Doug,

Klarmann Rulings does NIST traceability and certification for rulings,
micrometers, pinhole apertures, optical comparators, etc.

You can contact them at {www.reticles.com} or call them at 1-800-252-2401.
Their address is:

Klarmann Rulings
480 Charles Bancroft Highway
Litchfield, NH 03052

Bob Chiovetti


From daemon Tue Nov 12 20:02:15 2002



From: Marek Malecki, M.D., Ph.D., Professor :      MMalecki-at-wisc.edu
Date: Tue, 12 Nov 2002 12:52:40 -0800
Subject: RE: Clinical turn around

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Sharon and Tom:
In fact, Prof. Johanessen at Univ. of Bergen and later at Oslo published 4h
protocols (from biopsy taking to prints on the table) in the early 80s and
summarized in his basic book on ultrastructural pathology.
Cheers,
Marek.


At 05:58 PM 11/11/02 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Tue Nov 12 20:02:22 2002



From: Erasmus, Willem (WJ) :      willem.erasmus-at-sasol.com
Date: Tue, 12 Nov 2002 12:07:12 +0200
Subject: SEM particle size measurement

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Dear microscopists

I would like to estimate the particle size distribution of spherical particles that were embedded in a continuous solid phase and polished to obtain a cross-section. The cross-sectioning could have cut the particles at any point, which means that the size observed is probably not the size of the original particles.Is it possible to deduce the particle size distribution from measurements of the cross-sectional areas ? Can anyone suggest a good reference that may explain how this estimation is accomplished ?

Regards
W Erasmus

Willem Erasmus
Snr. Scientist, Materials Characterization Group
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]



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From daemon Tue Nov 12 20:07:44 2002



From: R. Ann Bliss :      bliss5-at-llnl.gov
Date: Tue, 12 Nov 2002 08:23:04 -0800
Subject: RE: TEM GaAs cross section

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Bruce;

Mike Bode's reply reminds me of the method my colleague uses for
cross sections. He first sandwiches the sample material between
pieces of silicon with G1 epoxy (Gatan) in a vise til the epoxy sets
up.Then the sandwich is waxed into a holder for a sonic cutter
(again, Gatan). The material cored out is epoxied into a brass tube
with a 3mm outside diameter.This tube is placed on a saw with a 3"
diameter diamond blade and several disks are cut. Now go to the lap,
dimple and ion mill steps.

I have no connection with Gatan; we happily use their products.

--

+++++++++++++++++++++++++++++

R. Ann Bliss
Technician, Chemistry and Materials Science
Materials Science and Technology Division
Lawrence Livermore National Laboratory

_____________________________



From daemon Tue Nov 12 20:16:11 2002



From: evgenia.pekarskaya-at-exxonmobil.com -at-sparc5.microscopy.com
Date: Tue, 12 Nov 2002 10:15:04 -0500
Subject: Re: SEM particle size measurement

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Hi Willem,

A good source of information is:
"Metallography. Principles and Practice" by George F. Vander Voort.

Regards,

Evgenia

**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355




"Erasmus, Willem
(WJ)" To: {Microscopy-at-sparc5.microscopy.com}
{willem.erasmus-at-sa cc:
sol.com} Subject: SEM particle size measurement


11/12/02 05:07 AM





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Dear microscopists

I would like to estimate the particle size distribution of spherical
particles that were embedded in a continuous solid phase and polished to
obtain a cross-section. The cross-sectioning could have cut the particles
at any point, which means that the size observed is probably not the size
of the original particles.Is it possible to deduce the particle size
distribution from measurements of the cross-sectional areas ? Can anyone
suggest a good reference that may explain how this estimation is
accomplished ?

Regards
W Erasmus

Willem Erasmus
Snr. Scientist, Materials Characterization Group
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]



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From daemon Tue Nov 12 20:26:21 2002



From: jerzy.gazda-at-amd.com -at-sparc5.microscopy.com
Date: Tue, 12 Nov 2002 13:20:16 -0600
Subject: SEM particle size measurement

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Willem,
A great start will be the "Stereology" and "The Image Processing Handbook" by John Russ. Check out Amazon.com or B&N.com. The stereological formula and examples are given there. Probably any other stereology text would also have the examples.

Have Fun,

Jerzy
******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************


-----Original Message-----
} From: Erasmus, Willem (WJ) [mailto:willem.erasmus-at-sasol.com]
Sent: Tuesday, November 12, 2002 4:07 AM
To: Microscopy-at-sparc5.microscopy.com



Dear microscopists

I would like to estimate the particle size distribution of spherical particles that were embedded in a continuous solid phase and polished to obtain a cross-section. The cross-sectioning could have cut the particles at any point, which means that the size observed is probably not the size of the original particles.Is it possible to deduce the particle size distribution from measurements of the cross-sectional areas ? Can anyone suggest a good reference that may explain how this estimation is accomplished ?

Regards
W Erasmus

Willem Erasmus
Snr. Scientist, Materials Characterization Group
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]



+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

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is subject to the standard Sasol eMail disclaimer which may be found at:
http://www.sasol.com/disclaimer.htm

If you cannot access the disclaimer through the URL attached and
you wish to receive a copy thereof please send an eMail to
disclaimer-at-sasol.com. You will receive the disclaimer by return eMail.






From daemon Tue Nov 12 20:27:10 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 12 Nov 2002 18:19:38 -0800
Subject: RMC MT6000-XL service needed

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Hello everybody

I need service for my RMC MT6000-XL ultramicrotome. It's located at UCLA,
Los Angeles, CA. If you could recommend real person/company I would really
appreciate. Thanks. Sergey

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Nov 12 20:29:31 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 12 Nov 2002 14:38:55 -0700
Subject: TEM GaAs cross section

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John is of course correct on both points: the steel could potentially
interfere with the magnetic field of the lenses and create unwanted effects.
I thought we were using steel (it's been a while), the material was
definitely not copper, but perhaps it was some other non-magnetic metal.

For the milling I always used LN2 cooling as John suggests, and I also used
Iodine. It helps to keep the milling as short as possible, so a careful
dimpling is important.

Thanks, John, for pointing that out.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Tuesday, November 12, 2002 10:15 AM
To: Bruce Brinson; MSA Listserver


Bruce;
I don't recommend using steel rings as supports, as these will
couple to the filed of the microscope lens and interfere with imaging. You
can get brass tube from Gatan (or from a hobby shop for a lot less). Also,
get vacuum tweezers if you don't want to fracture your samples. However, you
will have a long way to go even after that point. 3-5 compunds containing
indium and antimony dissociate during normal ion milling. You will need to
consult a series of papers written by Tony Cullis during the late '80s,
published in Ultramicroscopy, on the low temperature and iodine techniques
necessary to avoid a mess.

John Mardinly
Intel



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Monday, November 11, 2002 2:07 PM
To: MSA Listserver


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.




From daemon Tue Nov 12 20:32:50 2002



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Tue, 12 Nov 2002 11:09:22 -0600
Subject: Osmication - R-OTO technique

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I have just read Willingham & Rutherford (1984) J. Histochem Cytochm
32(4):455-460 paper on the use of ferrocyanide reduced osmium with the
osmium-thiocarbohydrazide-osmium (OTO) technique of Seligman. I thought
the conclusion that R-OTO gave a superior preservation and contrast of
membranes in TEM was a good one based on their images. But when I did a
Medline search for papers using either the OTO or R-OTO technique, the
limited number of papers was essentially limited to SEM work. Perhaps the
method is buried in papers and not coming up in a keyword search. Or maybe
no one uses it or there are problems with it?? Has any one used the R-OTO
(or even OTO) technique with tissue biopsies (Willingham & Rutherford use
cell cultures so it is tough to extrapolate the correct osmication
times)? Are there hidden pitfalls and disadvantages to this
technique? Any comments welcome. Tom


Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu





From daemon Tue Nov 12 20:36:00 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 12 Nov 2002 09:15:06 -0800
Subject: TEM GaAs cross section

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Bruce;
I don't recommend using steel rings as supports, as these will couple to the filed of the microscope lens and interfere with imaging. You can get brass tube from Gatan (or from a hobby shop for a lot less). Also, get vacuum tweezers if you don't want to fracture your samples. However, you will have a long way to go even after that point. 3-5 compunds containing indium and antimony dissociate during normal ion milling. You will need to consult a series of papers written by Tony Cullis during the late '80s, published in Ultramicroscopy, on the low temperature and iodine techniques necessary to avoid a mess.

John Mardinly
Intel



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Monday, November 11, 2002 2:07 PM
To: MSA Listserver


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.




From daemon Tue Nov 12 20:36:47 2002



From: William J Mushock :      wim5-at-lehigh.edu
Date: Mon, 11 Nov 2002 12:53:18 -0500
Subject: RE: vibration?

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Dear Bruce,

You might want to try the high angle polishing technique described in “The
Concept of High Angle Wedge Polishing and Thickness Monitoring in TEM Sample
Preparation,” Hao Li, and L. Salamanca-Riba, Ultramicroscopy 88, 171-8
(2001). With this technique you can make samples that have electron
transparent areas without any ion milling. Because of the high angle
polishing the samples are not very thin every where and, therefore, are less
likely to break.

I advise you to try the technique first with Si as it is a lot easier to
make a TEM sample of Si than InSb. When you try the InSb sample you should
use diamond paper with very fine particle size (3 microns) very early in the
polishing process because InSb is a very soft material. Then change to finer
particle size sooner than you would do with Si so that you can control
better how much material you are removing.

With this technique we were able to prepare cross sections of AlSb films
grown on GaSb substrates.

Good luck,

Lourdes Salamanca-Riba

----- Original Message -----
} From: "Bruce Brinson" {brinson-at-rice.edu}
To: "MSA Listserver" {microscopy-at-sparc5.microscopy.com}
Sent: Monday, November 11, 2002 5:06 PM


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You cannot generalize that the frequency of the interference on
the images is the same as that of the source.I participated in
an experiment several years ago in which we intentionally
generated an AC field at frequencies a few Hz above 60. The
resulting interference on the sem image was the difference between
the source and the 60Hz sync. In other words, a 65Hz field would look
like 5Hz(65-60). This also works for harmonics of 60Hz. A computer
monitor with a refresh rate of 75Hz would look like a 15Hz field
on an sem scan synched to 60Hz.

Also, the resonant frequency of the column suspension has to be
considered. Microscope columns usually have a resonant frequency
between 5-10Hz.




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My apologies to all - I made a couple of postings on this matter. The
first laid down the conditions I was using, assumptions that had to be
made
(I should have just asked the original poster, but what I wrote was
intended to be more general in application).

That original reply of mine laid out the assumption that the images were
long exposures. All later remarks I made were based on that. If,
instead,
these were short exposures then the frequencies involved could be 50 or
60
Hz. or higher. My claim that these image problems would be vibrations
was
based on those conditions.

Sorry if I caused any confusion on that point by not reiterating the
conditions in later messages..

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
ph. (630) 513-7093 fax (630) 513-7092 web http://www.sem.com


On Friday, November 08, 2002 6:57 AM, Vitaly Feingold
[SMTP:vitalylazar-at-worldnet.att.net] wrote:
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} ----- Original Message -----
} } From: Allen Sampson {ars-at-sem.com}
} To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
} {yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
} {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, November 08, 2002 6:43 AM
} Subject: RE: vibration?
}
}
} } Reminds me of the very old question of can a scanning electron spot on
a
} } CRT exceed the speed of light?
} }
} } If only it could be as easy to figure as you portray. I'll try to
explain
} } the situation, but it is really more complex that I can describe in
words
} } here - and being late at night, I really won't spend much time on the
} } matter.
} }
} } Let's assume that we are working at a working distance of 20mm. For
the
} } velocity you give for electrons accelerated at 2KV (27,000 kM/S) or
15KV
} } (73,000 kM/S), that 20mm would be traversed in an exceeding small
fraction
} } of a second. However, we aren't looking at the spatial translation of
a
} } single spot. In the SEM, the spot formed on the sample is constantly
} } moving - in the case of a record image the movement of the spot is
} } generally determined by the dwell time on each of the individual pixels
on
} } each line and the number and resolution of lines in the frame.
} }
} } In the record mode, most instruments will sync the start of each line
to
} } the zero-crossing of the electrical mains. This is an intentional
attempt
} } to minimize the effect of 50 or 60 Hz. interference. Digital systems
may
} } or may not do this.
} }
} } If you go vertically down the recorded image, you are actually looking
at
} } pixels that are separated by fairly large periods of time, 1/50th or
} 1/60th
} } of a second in the case of a synched record cycle. Now let's look at
your
} } projected travel times using these figures. The difference between the
} 2KV
} } and 15KV velocities you mention is 46000 kM/S. That gives us a
difference
} } in the velocity at these two accelerating voltages of 4.35x10-9 seconds
to
} } traverse the 20mm to the sample.
} }
}
} Correct.
}
}
} } Assuming a 60 Hz system, the difference between two vertically
separated
} } pixels is 0.0167 seconds.
}
}
} That is correct for a scan speed of 60 lines per second - frame of 1000
} lines will be scanned in 16 seconds.
}
}
} } Let's say that each 1/60th of a second is split
} } into 1500 equal parts - 1000 pixels used per line and 500 portions used
} for
} } the vertical retrace. That means that each pixel would be 0.000011
} seconds
} } apart.
}
}
} Adjacent horizontal pixels will be ~ 0.00001 seconds apart. While
adjacent
} vertical pixels will be 1/60s ~ 0.016s apart.
}
}
} } Now let's look at two pixels that are on separate scan lines and
separated
} } by one additional pixel. They would be separated by a time period of
} } 0.016678 seconds (adding the pixel dwell time to the line dwell time).
}
}
} Correct.
}
}
} } Doesn't sound like much, but in EM we have to understand the effects
of
} } the orders of magnitude we work with. If you divide the above time
} } difference to traverse the working distance by the pixel time
difference
} } just mentioned, you would find that there is a 2.58x10-7 second
difference
} } between the pixels mentioned above.
}
}
} Here is the juice. When you divide a time period by a time period, the
} result
} can not be time. It can be, however, a number of events, a number of
} portions, etc. What portions? The above figure of 2.58x10-7 is the
} difference
} between the portions of a pixel, which beam will scan across
} the specimen surface, equal to the time difference between 2kV and 15kV
} electrons crossing of the WD of 20 mm. Beam deflection (scan) times
remain
} the same for both accelerating voltages, of course.
} Can you see such difference on a micrograph? The ~ 3/10,000,000 of a
pixel?
} This will be the difference attributed to accelerating voltage change
from
} 2kV to 15kV and vice versa. This is why kV change will not bring any
} noticeable change into vibration affected image.
}
} My whole point was that scan (deflection) times/speeds are compatible
with
} times/speeds of both mechanical and AC magnetic
} events. The electron velocities/timing, in contrary, are not. They are
many
} orders of
} magnitude shorter/faster than mechanical events.
}
}
} }
} } I'm tired and really don't want to extend this too much more, but
consider
} } that the vibrations seen on the images at question cover around 20 or
more
} } scan lines and figure in the velocities of a nanometer scale RMS
} vibration.
} } You'll find that there is indeed a considerable, measurable, variation
in
} } image vibrations due to accelerating voltage (as well as working
} distance).
} }
} } Amazing, isn't it?
} }
} } By the way, I still claim that the frequency of the problems in the
} } original image is much lower than 50 or 60 Hertz and is the result of
} } vibrations rather than any electro-magnetic or electronic effect.
Another
} } poster mentioned the possibility that a turbo pump may be having
problems
} } but that is also not a likely cause as the frequencies involved don't
} } match. You might, however, want to check the operation of any water
} } chiller that is in use.
}
} Perhaps so, perhaps not. It can be chiller, or turbo pump, or anything
else.
} I can't say without more information.
}
}
} }
} } If I have learned anything in working on these instruments for well
over
} } twenty years, it's that nothing is simple or straightforward. We're
} } dealing with sub-atomic particle physics here folks, an area that can
only
} } be currently described by the statistical methods of quantum theories.
} }
} } As far as I am concerned, there is no further determination to be made
-
} } the problem is one of mechanical vibrations and I would be glad to
stake
} my
} } reputation on it, as I do on any posting I make here.
} }
} }
} } Allen R. Sampson
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} }
} } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
}
} This address can not receive messages larger than 15 kb without prior
} notification.
}
}
} }
} }
} } On Thursday, November 07, 2002 10:54 PM, Vitaly Feingold
} } [SMTP:vitalylazar-at-worldnet.att.net] wrote:
} } } Hey Allen,
} } }
} } } You may found the following figures entertaining. Natural
} } } constants and results of calculations are rounded to the first
character
} } } after the decimal point, relativity effects are neglected, plain text
} } format
} } } is used. I am a bit rusty on my Physics- my apologies if I missed an
} } order
} } } or so of magnitude. Makes no difference in this example.
} } }
} } } Electron mass ~ 9.1 x 10-31kg
} } } Electron charge ~ -1.6 x 10-19 C
} } } One electronvolt energy ~ 1.6 x 10-19J
} } } Kinetic energy (of electron, or anything having mass) = mV2/2, where
m
} is
} } } the mass,
} } } V2 is the velocity square.
} } }
} } } Simple calculations yield the following velocities in kilometers per
} } second
} } } for electrons accelerated by potential differences of:
} } } 1 Volt ~ 6 x 100 km/s
} } } 200 V ~ 8.4 x 1,000 km/s
} } } 2 kV ~ 2,7 x 10,000 km/s
} } } 15 kV ~ 7.3 x 10,000 km/s
} } }
} } } Amazing, isn't it?
} } }
} } } Mechanical vibration velocity must be compatible in magnitude with
the
} } above
} } } velocities, in order for vibration effects to look different at
} different
} } } beam accelerating voltages. But SEM is made of the materials found on
} } this
} } } planet, with limited durability specifications. Thus, in order to
} vibrate
} } } with such velocities, SEM must be either made very tiny, which is not
} the
} } } case, or must disintegrate into dust, but it doesn't.
} } }
} } } Velocities of mechanical vibrations affecting SEM are in many orders
of
} } } magnitude slower
} } } than the E-beam velocities.
} } }
} } } This is why SEM stage vibration will show up exactly the same at
} } different
} } } beam accelerating voltages.
} } }
} } } Why then stray magnetic field affects an e-beam? Because electron has
} } huge
} } } charge/mass ratio, ~ 10,000,000,000. The interaction is very strong.
} } Thus,
} } } effect increases at lower accelerating voltages. Same with working
} } distance-
} } } effect increases with the distance increase. But, one needs to change
WD
} } } substantially, to see the effect for sure. That will reduce the
} } resolution,
} } } among other things. I will advice against chamber/beam/sample
geometry
} } } change during troubleshooting process.
} } }
} } } Now down to business with Ann's images. The sawtooth vertical edges
} } } appearance is the result of horizontal scan being out of phase with
the
} } } external source of either vibration, fields, or signal interference.
} Time
} } } delay between the lines at slow scan is in order of tens to hundreds
} } } milliseconds, which is within the possible vibration frequency range.
} } Same
} } } frequencies will cause wavy image at TV deflection speeds.
} } }
} } } Another point is that mechanical vibration in many cases repeats the
AC
} } line
} } } frequency, as it is caused by electric motor driven devices, so
either
} 60
} } Hz
} } } or it's harmonic(s) are present as mechanical vibrations. The only
way
} to
} } } find the problem is to troubleshoot and differentiate.
} } }
} } } I agree with other postings- more information needed to develop more
} } } accurate idea on how to proceed.
} } }
} } }
} } } Vitaly Feingold
} } } Scientific Instruments and Applications
} } } 2773 Heath Lane, Duluth GA 30096
} } } (770)232-7785 ph.
} } } (770)232-1791 fax
} } } (678)467-0012 mobile
} } }
} } } This message is made of 100% recycled electrons.
} } }
} } } This address can not receive messages larger than 15 kb without prior
} } } notification.
} } }
} } } ----- Original Message -----
} } } From: Allen Sampson {ars-at-sem.com}
} } } To: 'Vitaly Feingold' {vitalylazar-at-worldnet.att.net} ; 'Ann-Fook Yang'
} } } {yanga-at-agr.gc.ca} ; 'microscopy-at-msa.microscopy.com'
} } } {Microscopy-at-sparc5.microscopy.com}
} } } Sent: Thursday, November 07, 2002 3:50 PM
} } } Subject: RE: vibration?
} } }
} } }
} } } }
} } --------------------------------------------------------------------
----
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
--------------------------------------------------------------------
} } ---.
} } } }
} } } }
} } } } Vitaly,
} } } }
} } } } Most of your concerns I covered in a previous reply. However, I
have
} } to
} } } } take exception to your claim that a large change in the problem's
} } } } characteristic would result from a change in accelerating voltage
only
} } if
} } } } the problem was electro-magnetic. A reduction in the accelerating
} } voltage
} } }
} } } } will also result in a reduction of the velocity of electrons in the
} } beam.
} } } } That would cause an increased displacement of the beam as it
} traverses
} } } the
} } } } sample surface due to vibrations.
} } } }
} } } } In other words, the effects of electro-magnetic interference and
} } } vibrations
} } } } are virtually inseparable except for the frequency involved. Each
} will
} } } } affect the beam in similar ways, in regards to accelerating voltage
} and
} } } } working distance. Both will have a greater affect with a lower
} } } } accelerating voltage as well as a greater working distance.
} } } }
} } } }
} } } } Allen R. Sampson
} } } } Advanced Research Systems
} } } } 317 North 4th. Street
} } } } St. Charles, Illinois 60174
} } } }
} } } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
} } } }
} } } }
} } } } On Wednesday, November 06, 2002 8:58 PM, Vitaly Feingold
} } } } [SMTP:vitalylazar-at-worldnet.att.net] wrote:
} } } } } Could be vibration. Could also be magnetic field. And, could be
} } signal
} } } } } interference, such as ground loop. What type of SEM are you
using?
} } } } }
} } } } } I will omit remedies for the vibration problem, since Allen
Sampson
} } did
} } } } } pretty good job addressing that.
} } } } }
} } } } } Stray AC line frequency magnetic field: try acquiring image at
} } } different
} } } } } accelerating voltages. It is very important that all other
geometry
} } } } } parameters will remain the same: particular area of a particular
} } } } specimen,
} } } } } WD, tilt, magnification, spot size. Contrast, brightness, focus,
and
} } } } } stigmator settings can be changed. If the problem is much worse
at,
} } say,
} } } } 2
} } } } } kV, than at, say, 15kV, you are dealing with magnetic field. You
may
} } } also
} } } } } try to keep 3 axes AC milliGauss meter at the SEM, and notice
what
} it
} } } } } measures, but catching stray magnetic interference this way may
be a
} } } } little
} } } } } tricky. These meters, though, are very inexpensive, and available
} } from
} } } } many
} } } } } test instruments suppliers.
} } } } }
} } } } } Now we are getting down to statistically most likely problem-
signal
} } } } } interference and ground loops. It is difficult to go any further
} } without
} } }
} } } } } knowing the SEM type, and acquisition system (if separate) type.
Too
} } } many
} } } } } possibilities exist, but the very first- does your acquisition
} } software
} } } } } have an option somewhere in the settings which reads something
like
} } "60
} } } } } Hertz sync", or "AC line synchronization", or "Line sync", etc.,
} } etc.?
} } } If
} } } } } yes, check (or uncheck) that option and see what happens. Further
} } } } remedies
} } } } } of this problem will include eliminating ground loops. Example-
sh
} } ielded
} } } } } cable(s) has shield connected on both sides, and shield is used
as
} } one
} } } of
} } } } } the signal wires. This is frequently done, and does jeopardize
} } } instrument
} } } } } interference tolerance. Another example- one of the accessories
is
} } } } } susceptible to some kind of interference, and must be powered
} through
} } } the
} } } } } isolation transformer. The latter remedy will only work with
decent
} } } } (better
} } } } } if dedicated) ground. The fact that the problem is not present
all
} } the
} } } } time
} } } } } does not mean that everything is perfect inside the SEM. Besides,
} } going
} } } } } after the EM interference source could be inefficient and
} } frustrating,
} } } if
} } } } } not impossible. Improving grounding/shielding/power connection
might
} } be
} } } } } easier.
} } } } }
} } } } }
} } } } } Vitaly Feingold
} } } } } Scientific Instruments and Applications
} } } } } 2773 Heath Lane, Duluth GA 30096
} } } } } (770)232-7785 ph.
} } } } } (770)232-1791 fax
} } } } } (678)467-0012 mobile
} } } } }
} } } } } This message is made of 100% recycled electrons.
} } } } }
} } } } } This address can not receive messages larger than 15 kb without
} prior
} } } } } notification.
} } } } }
} } } } } ----- Original Message -----
} } } } } } From: Ann-Fook Yang {yanga-at-agr.gc.ca}
} } } } } To: {microscopy-at-sparc5.microscopy.com}
} } } } } Sent: Wednesday, November 06, 2002 2:45 PM
} } } } } Subject: vibration?
} } } } }
} } } } }
} } } } } }
} } } }
} }
------------------------------------------------------------------------
} } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
of
} } } } America
} } } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } }
} } } }
} }
-----------------------------------------------------------------------.
} } } } } }
} } } } } }
} } } } } } Hi everyone,
} } } } } }
} } } } } } I have two SEM micrographs on the web:
} } } } } } http://www.magma.ca/~scimat/Defect.htm
} } } } } } The micrographs show zagged edges taken at 20 kX. Such
phenomenon
} } } does
} } } } } not present all the time. Can anyone suggest what causes the
problem
} } and
} } } } how
} } } } } to solve it?
} } } } } } Thanking in advance.
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } } AnnFook Yang
} } } } } } EM Unit,
} } } } } } Eastern Cereal and Oilseed Research Centre,
} } } } } } Room 2091, Bldg. 20,
} } } } } } Central Experimental Farm,
} } } } } } Ottawa, Ontario
} } } } } } Canada K1A 0C6
} } } } } }
} } } } } } Tel: 1-613-759-1638
} } } } } } Fax: 1-613-759-1701
} } } } } }
} } } } } } e-mail: yanga-at-em.agr.ca
} } } } } }
} } } } } }
} } } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } }
} } } }
} } }
} } }
} } }
} }
}
}
}
}
}
}
}



From daemon Tue Nov 12 22:35:30 2002



From: paul r hazelton, PhD :      Paul_Hazelton-at-umanitoba.ca
Date: Tue, 12 Nov 2002 22:27:30 -0600
Subject: Re: Osmication - R-OTO technique

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tom

tried it with both cell suspensions and excised tissue. it did not work

at all well in my hands.

paul hazelton






From daemon Tue Nov 12 23:08:26 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 11 Nov 2002 12:16:29 -0700
Subject: RE: vibration?

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Yep, as long as you can't transmit any information between the spot
positions faster than light, you can WARP speed on them.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Dusevich, Vladimir [mailto:dusevichv-at-umkc.edu]
Sent: Monday, November 11, 2002 7:29 AM
To: microscopy-at-msa.microscopy.com



}
} If anyone gets an electron to travel faster than the speed of
} light, please
} copy me.
}
} Peter

An electron and a scanning electron spot are two very different
things. There are no lows preventing the spot from traveling with
any speed.

Vladimir



From daemon Tue Nov 12 23:32:50 2002



From: Oparowski, Joseph :      Joseph_Oparowski-at-bose.com
Date: Mon, 11 Nov 2002 07:25:15 -0500
Subject: oxygen monitor

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Pat,

We have a small exhaust equipped gas storage room where we fill LN2 dewars.
We keep the doors in the area open when we fill. If the flow rate is
moderate, we do not have a problem. However, if we get an occasional med
pressure dewar, the alarm will sound. The sensor is about 5 feet off the
floor. Hope this helps.


Joseph

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313


-----Original Message-----
} From: psconnel-at-sas.upenn.edu [mailto:psconnel-at-sas.upenn.edu]
Sent: Monday, November 11, 2002 7:53 PM
To: Oparowski, Joseph
Cc: Microscopy-at-sparc5.microscopy.com


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Jonathan,

We use a handheld O2 monitor system from Draeger (PAC III). It is good for
at least two years and costs under $1K with a charging stand.


Joseph

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313


-----Original Message-----
} From: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Friday, November 08, 2002 2:05 PM
To: Microscopy-at-sparc5.microscopy.com


Hi:

I am thinking I should be more careful with our liquid nitrogen handling. I
would like to monitor oxygen levels in the room we fill dewars.

So far, I have found small, battery operated low oxygen alarms for about
$300. But, they only last a year before needing replacement. The permanent
installations I have found go for over $1K.

Is this right? Any one with some sage advice? Yes, I know better safe than
sorry, but just double checking with the knowledge base.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Wed Nov 13 00:34:45 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 11 Nov 2002 17:35:59 -0700
Subject: TEM GaAs cross section

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Bruce,

try this one. I used to do this on a regular basis when I was still doing
TEM. We looked at anything from heterostructures to dislocations, and had a
good success with this technique:

1) buy some 3mm OD steel pipe (or perhaps other strong material)

2) cut rings with a thickness of half a mm to a mm off the pipe. Clean rings
thorougly and remove burrs.

3) with the rest of the pipe, drill a ring shaped groove into the material
you are interested in around the part you want to prepare.

4) Glue with a strong epoxy one of the rings into the groove.

5) grind sample from backside until you have a freestanding steel ring with
the GaAs or other material inside.

6) Dimple

7) Ion mill

8) Enjoy.

the steel ring gives it enough support for handling with tweezers. You
probably need to experiment with the thickness of the rings and other
parameters, but it works, once you got everything under control.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Monday, November 11, 2002 3:07 PM
To: MSA Listserver


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.




From daemon Wed Nov 13 00:34:45 2002



From: DrJohnRuss-at-aol.com -at-sparc5.microscopy.com
Date: Tue, 12 Nov 2002 08:03:03 EST
Subject: Re: SEM particle size measurement

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In a message dated 11/12/02 5:42:19 AM, willem.erasmus-at-sasol.com writes:

} I would like to estimate the particle size distribution of spherical
particles
} that were embedded in a continuous solid phase and polished to obtain a
} cross-section. The cross-sectioning could have cut the particles at any
} point, which means that the size observed is probably not the size of the
} original particles.Is it possible to deduce the particle size distribution
} from measurements of the cross-sectional areas ? Can anyone suggest a good
} reference that may explain how this estimation is accomplished ?
}
}
}
} Regards
}
} W Erasmus

You can find the explanation and math in Practical Stereology, 2nd edition,
J. C. Russ & R. T. Dehoff, Plenum Press, 2001. Assuming that you have a way
to measure the intersection sizes, the calculations can be done in a
spreadsheet. However be warned that the technique, which has been known and
used since the 1920's, has two problems. First, it is critically dependent on
the assumption that the shape of the features is exactly known and that it
remains the same for large and small features. Second, it is mathematically
unstable, with the precision of the 3D data being much poorer than that of
the 2D intersections. These problems have caused many stereologists to prefer
another approach that provides the mean and standard deviation of the
distribution without making any shape assumptions. that method is also
described in the book. Original references to important papers are provided
for all of the stereological techniques.

John Russ



From daemon Wed Nov 13 00:49:05 2002



From: Bruce Brinson :      brinson-at-rice.edu
Date: Mon, 11 Nov 2002 14:06:37 -0800
Subject: TEM GaAs cross section

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Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.




From daemon Wed Nov 13 00:49:05 2002



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Wed, 13 Nov 2002 07:43:39 +0100
Subject: Re: SEM particle size measurement

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Hi

You will find a description of what you want in the book "Morphometry" by
Aherne WA and Dunnill MS, Pub. Arnold, ISBN 0 7131 4538 2. It is out of
print but Amazon are selling it second hand at

http://www.amazon.com/

I would be interested in knowing what other suggestions people come up with.


At 12:07 2002-11-12 +0200, Erasmus, Willem (WJ) wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Obs/NB New postal/visiting address from July 2002!

Med vänliga hälsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 5858 1038
Fax +46 728 3747

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Huddinge Universitetssjukhus, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.



From daemon Wed Nov 13 00:55:21 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 11 Nov 2002 22:08:55 -0500
Subject: TEM GaAs cross section

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I highly recommend the small angle cleavage technique for III-V materials. It is a very easy technique to learn, produces superior samples to any other technique, and it is very quick, approx. 9 samples per hour. Out of that 9 samples, about 5-7 will be usable and 2-3 of those will be fantastic. Go to the South Bay Technology web page and check out their microcleave kit. There are some examples of a MQW GaAs-InGaAs structure that I prepared in their document section along with some other examples. Send me your address, and I can send you a disk with a detailed poster on how to do it. You can also go to the #4 TEM sample prep book from MRS proceedings. John McCaffrey and I have a detailed discussion on how to do it.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Monday, November 11, 2002 5:07 PM
To: MSA Listserver


Hello TEM Microscopist and sample prep masters,

I've spent a humbling amount of time trying to learn to prepare TEM
cross section samples from GaAs substrates. I should also point out that
I do not have mentor for this project. For the moment I am working with
dummy samples. When I start working with the real material of interest
(InSb AlAs quantum wells), we will typically have enough material for
one attempt... so I need to get the yield (to the TEM) up to or atleast
close to 1.
I am able to block, polish and dimple the material easily enough but
loose many samples transferring from the dimpling block (glass post) to
the Cu support.

My procedure is .... then
1. dimple on a glass post.
2. I then put a piece of filter paper and stack 2 u-scope slides in a
dish. I put the post, sample down, with one edge of the post on the
u-scope slides allowing a space for the dimpled bit to fall off then
immerse in acetone .
To this point my yield is ~1

Mounting to the Cu support is where I often loose the samples due to
fracturing. Use fine tipped tweezes typical of those use with 3mm TEM
grids and all the caution I can muster. I lift the sample out and
attempt to lay it on a glue bearing support. Some times I get there in 1
piece, sometimes I don't.

Since we need a yield of 1 for 30+ samples this is a real problem.

Any advice on the sample prep. of this material would be greatly
appreciated.


Bruce Brinson
Rice U.




From daemon Wed Nov 13 01:06:09 2002



From: MicroscopyToday :      microtod-at-optonline.net
Date: Mon, 11 Nov 2002 13:57:12 -0500
Subject: Re: Sectioning of Lowicryl K4M embedded sample

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Alan,

You've made a great point that would be of considerable interest to many
microscopists.

I would love to see this discussion ensue and then a final wrap-up
written for publication in Microscopy Today!

Ron Anderson, Editor
Microscopy Today

-----Original Message-----
} From: Allan Mitchell [mailto:allan.mitchell-at-stonebow.otago.ac.nz]
Sent: Sunday, November 10, 2002 2:42 PM
To: Microscopy-at-sparc5.microscopy.com
Cc: phillipst-at-missouri.edu


Hi Tom, and others

You have raised an interesting point that I have never really been
able to get a handle on satisfactorily, that is how do you choose the
appropriate acrylic resin to use for a new project,

eg,

should I choose LR White or LR Gold for project X, should I
chemically polymerise or perhaps use UV ?

or maybe I should try Unicryl, or Lowicryl, but which one HM20, K4M etc.

Are there others I should be considering?

I would be interested in seeing a discussion on how different people
go about choosing a particular acrylic resin for a new project they
may have to undertake.

Regards

Allan


} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America


From daemon Wed Nov 13 05:12:54 2002



From: =?iso-8859-1?Q?Dal=E9ne?= Josling :      djosling-at-op.up.ac.za
Date: Wed, 13 Nov 2002 12:54:43 +0200
Subject: Post Mortem Perfusion fixation of animal organs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good day

Can anyone maybe help with this question from one of our post graduate
students?

Hello,

My name is Susanne Schmidt, am an Austrian Veterinarian and I am doing
my post grad studies at Oderstepoort university.
I do research on the histology of the uterus of non pregnant and
pregnant cows. For fixation of the uterus/placenta I want to use a
perfusion method to make sure that maternal and fetal membranes stay
well together. As a fixative I will use Glutaraldehyde 2,5% in 0,013M
Millonig's buffer and I will perfuse the organ through the uterine
arteries, leaving the veins open. At the same time I will fill the
amniotic cavity with fixative to insure immersion through the fetal
placental membranes.
The physiological blood pressure in a cow is +/- 145 mmHg (=0,15bar). I
already tried to perfuse the uterus (after perfusing with saline to get
rid of blood...) , using a infusion bag filled with gluytaraldehyde
hanging up at a height of 2 m above the organ. (I perfused through both
arteries for about 15-20 min) I used tubes which are normaly used for
a drip in horses and 18G or 20 G needles,depending on the thickness of
the arteries. The result was not as I had expected and I have the
feeling that the "blood"-pressure was not heigh enough .
My question now is:
How can I ensure that the fixative runs through the organ with a
pressure of more or less 0,2 bar! (or do I need more ??)

Are there any other possibilities to make sure that maternal and fetal
membranes stay together when I take my samples so that I can have a good
picture of the foeto-maternal junction??

Thank you very much for you advice and help!!!
Please answer to following adress: Susanne-at-op.up.ac.za

Regards
Susanne Schmidt






From daemon Wed Nov 13 07:37:59 2002



From: Ann-Fook Yang :      yanga-at-agr.gc.ca
Date: Wed, 13 Nov 2002 08:27:21 -0500
Subject: =?ISO-8859-7?Q?vibrations=3F=20=AF=20update?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to those who responded to my posting.
There were all kinds of suggestions: from vibration, field interference, faulty ground. Some are more specific e.g. chilled water pulsing, bad turbo pump, faulty digital system, electrical motor or monitor nearby.

I am glad that my colleague, Milos Kalab has found the cause of the problem. It was the multiple stage he had used being loose. It explans why the fault was not there all the time. In addition, thanks to the service rep, Frank Shapiro who found the vibration caused by pulsing chilled water at a higher mag.

Milos Kalab is very please with all the suggestions and would like to put them on his website. If any one of you do not want to be identified, please let me know. I will provide the ERL when it is ready.



AnnFook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Room 2091, Bldg. 20,
Central Experimental Farm,
Ottawa, Ontario
Canada K1A 0C6

Tel: 1-613-759-1638
Fax: 1-613-759-1701

e-mail: yanga-at-em.agr.ca



From daemon Wed Nov 13 07:52:56 2002



From: Smartech :      smartech-at-optonline.net
Date: Wed, 13 Nov 2002 08:58:58 -0500
Subject: Summary to : Re: I need help with digital images __ How to subtract a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Sergey:

RMC-Boeckeler is still in business in Tucson Arizona. The 6000xl was
declared obsolete by RMC-Ventana in 1999 because lack of spare parts.
Limited service might be available from RMC depending what is wrong with the
instrument.

Please contact: Dave Roberts or Greg Becker at 520-745-0001
Email: Dave-at-boeckeler.com or Greg-at-boeckeler.com

Best,

Al Coritz, Sales Manager
Electron Microscopy Sciences & Diatome


----- Original Message -----
} From: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, November 12, 2002 9:19 PM


Thanks for all the help with working with gradients.

I ended up using adobe gaussian blurr function with 2 pixel to get rid of
fine data and 50 pixels to generate a background image. I then down loaded
imagej (http://rsb.info.nih.gov/ij/) to do the image math. I ended up
adding the inverted background to the original image and was than able to
then enhance the contrast of the subtle pattern. I also cropped edges and
annotation out before I did the routine. I have to say imagej is amazing.

Ric

SMARTech
860-485-5054
www.semguy.com
19 Cornwall Drive
Goshen CT 06756




From daemon Wed Nov 13 09:45:40 2002



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 13 Nov 2002 10:35:30 -0500
Subject: NESM Fall Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:

The 36th Annual Fall Symposium of the New England Society for Microscopy
(NESM) will be held on Friday, December 6th at Gordon College in Wenham, MA.

The meeting begins at 12Noon with registration in the Lane Student Center.
There are 3 Sessions: Session 1 is devoted to four 15-minute student
presentations. Session 2, the Biological Session will feature 2 talks on
Confocal Microscopy and Session 3, the Materials Science Session will
feature 2 talks, one of which is by Lucille Giannuzzi, the MAS Tour Speaker.

Following the technical talks, NESM will hold it's annual business meeting.
Dinner will follow at 6:00pm and then Debora Mayer, from the Mayer
Conservation Studio in Portsmouth, NH will speak on "Fiber Analysis and
Art".

Details regarding registration, speakers/topics, and directions to Gordon
College can be found on NESM's website: http://prism.mit.edu:8083. Further
information on Gordon College (including a map of the campus) can be found
on it's website: http://www.gordon.edu.

The deadline for advance registration (and dinner) for this meeting is
Friday, November 29th.

Please join us for this most interesting meeting!

Peggy Sherwood
Corresponding Secretary/Newsletter Editor, NESM

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



From daemon Wed Nov 13 09:51:27 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 13 Nov 2002 10:41:55 -0500
Subject: Re: RMC MT6000-XL service needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} Hello everybody
}
} I need service for my RMC MT6000-XL ultramicrotome. It's located at
} UCLA, Los Angeles, CA. If you could recommend real person/company I
} would really appreciate. Thanks. Sergey
}
} _____________________________________

Sergey,
RMC is now part of BoeckelerInstruments in Tucson AZ. You can
contact Dave Roberts or Greg Becker at 502-745-0001.
They may still be supporting the 6000 series.
Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Nov 13 10:51:29 2002



From: Thearith H. Ung :      tung-at-qdots.com
Date: Tue, 12 Nov 2002 10:48:12 -0800
Subject: TEM Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Karen,

I have been using a CCD which I purchased from a company called TVIPS. Their
cameras are very easy to install and easy to use. You can contact Hans
Tietz, the president of the company, at the address below:

hans.tietz-at-tvips.com

Regards,
Thearith

------------------

Thearith Ung

Quantum Dot Corporation
26118 Research Road
Hayward, CA 94545, USA
Tel: 510-887-8775 (Ext 4125)
Fax: 510-783-9729
Webiste: www.qdots.com

--------------------


-----Original Message-----
} From: Jensen, Karen [mailto:Karen_Jensen-at-urmc.rochester.edu]
Sent: Monday, November 11, 2002 1:43 PM
To: 'microscopy-at-msa.microscopy.com'


Dear Listers:

We are planning to upgrade our TEM digital camera purchased 9 years ago
from. We do alot of kidney biopsies as well as various research specimens.
Does anyone have any experience with the higher resolution TEM digital
cameras? We are looking for something around 2-4 megapixels.

Thanks

Karen

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954



From daemon Wed Nov 13 12:01:26 2002



From: Martin Ramirez :      ramirez-at-amnh.org
Date: Wed, 13 Nov 2002 12:55:52 -0500
Subject: CPD models

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

I have to buy a critical point dryer for my new lab in Argentina, and
thought that the EMS 850 could be a good option. I will very much
appreciate any tip from anyone who knows that CPD, or another model that
could recommend instead. Please reply to my personal address
{ramirez-at-amnh.org} . Thanks! Martin


Martin J. Ramirez
Division of Invertebrate Zoology
American Museum of Natural History
Central Park West at 79th Street
New York 10024, N.Y.
USA
Tel: (212) 769 5609
Fax: (212) 769 5277
email: ramirez-at-amnh.org



From daemon Wed Nov 13 12:32:32 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 13 Nov 2002 10:23:17 -0800
Subject: Re: RMC MT6000-XL service needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hello everybody
}
} I need service for my RMC MT6000-XL ultramicrotome. It's located at UCLA,
} Los Angeles, CA. If you could recommend real person/company I would really
} appreciate. Thanks. Sergey
}
} _____________________________________
}
} Sergey -

Contact RMC! dave-at-boeckeler.com

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Nov 13 13:13:23 2002



From: Shanling Shi :      Shanling.Shi-at-unilever.com
Date: Wed, 13 Nov 2002 14:02:11 -0500
Subject: Re: Sectioning of Lowicryl K4M embedded sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear lister,

Many thanks for all help I got from this list. Thanks!!!

Actually I have been trying all different kind of resins for my immunolabeling
experiments. I have same question as Allan has. Which one is right resin to
use? A book I received today is very informative about choosing acrylic resins.
The title is : Resin Microsocpy and On-Section Immuno-ctyochemistry, second
edition, by G. R. Newman and J. A. Hobot, 2001, Springer, (ISBN 3-540-67277-X).

Shanling

-----Original Message-----
} From: Allan Mitchell [SMTP:allan.mitchell-at-stonebow.otago.ac.nz]
Sent: Sunday, November 10, 2002 2:52 PM
To: Microscopy-at-sparc5.microscopy.com
Cc: phillipst-at-missouri.edu


Hi Tom, and others

You have raised an interesting point that I have never really been
able to get a handle on satisfactorily, that is how do you choose the
appropriate acrylic resin to use for a new project,

eg,

should I choose LR White or LR Gold for project X, should I
chemically polymerise or perhaps use UV ?

or maybe I should try Unicryl, or Lowicryl, but which one HM20, K4M etc.

Are there others I should be considering?

I would be interested in seeing a discussion on how different people
go about choosing a particular acrylic resin for a new project they
may have to undertake.

Regards

Allan


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"




From daemon Wed Nov 13 13:49:55 2002



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Wed, 13 Nov 2002 13:34:35 -0600
Subject: Re: RMC MT6000-XL service needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:41 AM 11/13/2002 -0500, Leona Cohen-Gould wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

They do not support the MT6000 series. When we had a problem we were told
it was obsolete and we would need to but a new microtome.


Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Wed Nov 13 14:09:58 2002



From: Martin Ramirez :      ramirez-at-amnh.org
Date: Wed, 13 Nov 2002 15:08:05 -0500
Subject: CPD models

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

I have to buy a critical point dryer for my new lab in Argentina, and
thought that the EMS 850 could be a good option. I will very much
appreciate any tip from anyone who knows that CPD, or another model that
could recommend instead. Please reply to my personal address
{ramirez-at-amnh.org} . Thanks! Martin


Martin J. Ramirez
Division of Invertebrate Zoology
American Museum of Natural History
Central Park West at 79th Street
New York 10024, N.Y.
USA
Tel: (212) 769 5609
Fax: (212) 769 5277
email: ramirez-at-amnh.org



From daemon Wed Nov 13 15:52:30 2002



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 13 Nov 2002 15:45:53 -0600
Subject: 4% Noble Agar Embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm trying to work out the method for embedding glutaraldehyde fixed
centrifuged urine to embed in 4% Noble Agar, such that the specimen can be
blocked in Epon-Araldite for sectioning to hunt for Human Polyoma Virus
infection in transplant recipients.

} From the paper that I've referred to, they fail to mention the details of
blocking in the 4% Agar embedding, and I can't remember those critical
temperatures to get the agar into just the right condition for embedding -
ie: not too hot, and not too cold.

Can anyone remind me of this Agar procedure?

Garry



From daemon Wed Nov 13 19:16:41 2002



From: Mary McKee :      mckee-at-helix.mgh.harvard.edu (by way of
Date: Wed, 13 Nov 2002 17:08:23 -0800
Subject: Zeiss Axioplan replacement parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Help!

I've been asked to see if I can find the following for a Zeiss Axioplan
fluorescent microscope ASAP.
Power supply, collector lens, lamp housing and socket. Does anyone have any
leads? Thanks.

Mary

Mary McKee
MGH Renal Unit
Bldg. 149, Rm. 8113
149 13th St.
Charlestown, MA 02129

(617)726-3696 (phone)
(617)726-5669 (fax)

Fees for EM Services: $20.00/hr*
$30.00/hr, if you watch
$40.00/hr, if you help
$50.00/hr, if you tried to do
it first, and couldn't


From daemon Wed Nov 13 19:16:44 2002



From: Susanne Brandom :      spb-at-microscopy.info (by way of
Date: Wed, 13 Nov 2002 17:08:48 -0800
Subject: Need video microscopy for documentary in Wash DC area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If anyone can help, please respond to Carlos Salcedo
[mailto:centurion1_2000-at-yahoo.com]

Thank you

Susanne Brandom
microscopy.info

-----Original Message-----
} From: Carlos Salcedo [mailto:centurion1_2000-at-yahoo.com]
Sent: Friday, November 08, 2002 1:26 PM
To: spb-at-mwrn.com


I work at American University in the documentary film
dept. We are looking ofr someone that can do video
microscopy in the WDC area. We are trying to fotograph
moving images of cell replication in the microscope
environment for a cancer documentary.

Do you know anyone/agency/service provider that can
handle this type of request?

We have a budget for this. We just can't find the
service. NIH has it but they don't accept outside
requests?

Thanks.

Carlos Salcedo
American University
240-899-1404


From daemon Wed Nov 13 22:13:08 2002



From: adultmatchnetwork4322x20-at-vol.com
Date: Wed, 13 Nov 2002 17:42:06 +1000
Subject: Married But Cheating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For the men:
*Currently, there are 42,736 married women in the U.S. who are lonley and looking for some fun.

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*Currently, there are 26,085 married men in the U.S. who are lonley and looking for some fun.

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From daemon Wed Nov 13 22:48:26 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 13 Nov 2002 20:46:29 -0800
Subject: Re: Need video microscopy for documentary in Wash DC area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Why doesn't the NIH do a CRADA (Cooperative Research
and Development Agreement) with you under the Stevenson-Wydler
Technology Transfer Act of 1986?

If they wanted to, it seems to me that the work could likely be fit in.

gary g.



At 05:08 PM 11/13/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Nov 14 02:19:37 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 14 Nov 2002 08:10:12 +0000 (GMT Standard Time)
Subject: Re: 4% Noble Agar Embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use Agarose Type 1X which sets slowly at room
temperature. This gives you more time to add your cells
before it sets. My protocol is given below.

Dave

Enrobing cells in Agarose

When dehydrating cells one needs to centrifuge between
exchanges. Cells can be lost at each stage. A solution is
to enrobe the cells in agarose and thereafter treat them
like tissue samples.

Fix, rinse in buffer and osmicate cells

Rinse 3 x 1m in distilled water

Dissolve 0.3g agarose (type IX ultra-low gelling
temperature, Sigma, A-5030) in 10ml distilled water. Use
hotplate and magnetic stirrer

Centifuge agarose and cells

Place in fridge for 15m (agarose will gel below 15 degrees
C)

Cut end off Eppendorf with razor blade

Place remaining cone upright and cut vertically

Scoop out sample with small spatula and mounted needle

Place in distilled water and start dehydration





On Wed, 13 Nov 2002 15:45:53 -0600 Garry Burgess
{GBurgess-at-exchange.hsc.mb.ca} wrote:

}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
Society of America } To Subscribe/Unsubscribe -- Send
Email to ListServer-at-MSA.Microscopy.Com } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
} } I'm trying to work out the method for embedding
glutaraldehyde fixed } centrifuged urine to embed in 4%
Noble Agar, such that the specimen can be } blocked in
Epon-Araldite for sectioning to hunt for Human Polyoma
Virus } infection in transplant recipients.
} } } From the paper that I've referred to, they fail to
mention the details of } blocking in the 4% Agar embedding,
and I can't remember those critical } temperatures to get
the agar into just the right condition for embedding - }
ie: not too hot, and not too cold. }
} Can anyone remind me of this Agar procedure? }
} Garry }
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Nov 14 03:48:57 2002



From: Francisco J. Hernandez Blazquez :      fjhblazq-at-usp.br
Date: Thu, 14 Nov 2002 07:50:01 -0200
Subject: Re: Osmication - R-OTO technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear Sir:
You are right, it is not easy to find references about the OTO method, but it works very well in transmission electronmicroscopy studies. It was a method used by people who studied intestinal absorption in fishes in the 70´s and 80´s as it was my case. I suggest that you look at the original paper of Seligman et al., 1966, Journal of Cell Biology 30:424-432. I´ve used this method in my associate professor thesis and I was succesful at first try I just followed the recipe of the paper.
My objective was to demonstrate quilomicra and lipid droplets in the enterocytes of an antarctic fish. This paper was not yet published.


Prof. Dr. Francisco Javier Hernandez Blazquez
Departament of Surgery - Sector of Anatomy
Faculty of Veterinary Medicine and Animal Sciences
University of São Paulo
Av. Prof. Dr. Orlando Marques de Paiva, 87
05508-000 - São Paulo (SP) - Brazil
Tel..55 (11) 3091 1374 Fax 55 (11) 3091 7805
email: fjhblazq-at-usp.br



At 11:09 12/11/02 -0600, Tom Phillips wrote:

} I have just read Willingham & Rutherford (1984) J. Histochem Cytochm 32(4):455-460 paper on the use of ferrocyanide reduced osmium with the osmium-thiocarbohydrazide-osmium (OTO) technique of Seligman. I thought the conclusion that R-OTO gave a superior preservation and contrast of membranes in TEM was a good one based on their images. But when I did a Medline search for papers using either the OTO or R-OTO technique, the limited number of papers was essentially limited to SEM work. Perhaps the method is buried in papers and not coming up in a keyword search. Or maybe no one uses it or there are problems with it?? Has any one used the R-OTO (or even OTO) technique with tissue biopsies (Willingham & Rutherford use cell cultures so it is tough to extrapolate the correct osmication times)? Are there hidden pitfalls and disadvantages to this technique? Any comments welcome. Tom
}
}
} Thomas E. Phillips, PhD
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu




From daemon Thu Nov 14 09:05:43 2002



From: Nancy Smythe :      smythen-at-musc.edu
Date: Thu, 14 Nov 2002 09:48:27 -0500
Subject: I use the OTO method on about half of my specimens. I have

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use the OTO method on about half of my specimens. I have only had one
time that things didn't work and I got a bad precipatate on the cells. I
follow the Willingham technique. The temp of the solutions does seem to
be very important. If I can help please let me know.

Nancy Smythe, Research Specialist
Medical University of South Carolina
Charleston, SC


From daemon Thu Nov 14 09:27:17 2002



From: joe fu :      jofu-at-nist.gov
Date: Thu, 14 Nov 2002 10:19:46 -0500
Subject: Compatible camera for UHV

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues:
I am interesting in cameras that are compatible in the UHV. Do they
exist? Most of SEMs today equipped with a camera inside of vacuum
chamber, however you don't see these cameras inside of the UHV system. Any
solution??? Thank you.

Joseph Fu
National Institute of Standards & Technology
100 Bureau drive Stop 8212
Gaithersburg, MD. 20899-8212
Tel: 301-975-3495
Fax: 301-869-0822
Email: jofu-at-nist.gov




From daemon Thu Nov 14 09:34:57 2002



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Thu, 14 Nov 2002 10:25:23 -0500
Subject: paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi group - anybody out there working on layers of paint samples?
Looking to distinguish layers by wavelength. Apparently this party has
already used SEM and optic scopes without success. Guess density is to
close to distinguish layers. We have been told that a PanaCL could do
the trick - would appreciate any suggestions.
Thanks
Barb



From daemon Thu Nov 14 10:53:33 2002



From: Holly Aaron :      hollya-at-socrates.berkeley.edu
Date: Thu, 14 Nov 2002 08:43:18 -0800
Subject: RE: Zeiss Axioplan replacement parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Mary -

You can try calling the Zeiss Spare Parts Dept. in NY:
1-800 633 6610 and then the Spare Parts extension.

Good luck,
Holly

Holly L. Aaron
CRL Molecular Imaging Center
http://imaging.berkeley.edu

} -----Original Message-----
} From: Mary McKee (by way of MicroscopyListserver)
} [mailto:mckee-at-helix.mgh.harvard.edu]
} Sent: Wednesday, November 13, 2002 5:08 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Zeiss Axioplan replacement parts
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} Help!
}
} I've been asked to see if I can find the following for a Zeiss Axioplan
} fluorescent microscope ASAP.
} Power supply, collector lens, lamp housing and socket. Does
} anyone have any
} leads? Thanks.
}
} Mary
}
} Mary McKee
} MGH Renal Unit
} Bldg. 149, Rm. 8113
} 149 13th St.
} Charlestown, MA 02129
}
} (617)726-3696 (phone)
} (617)726-5669 (fax)
}
} Fees for EM Services: $20.00/hr*
} $30.00/hr, if you watch
} $40.00/hr, if you help
} $50.00/hr, if you tried to do
} it first, and couldn't
}
}


From daemon Thu Nov 14 12:52:10 2002



From: ian davenport :      iand-at-CLEMSON.EDU
Date: Thu, 14 Nov 2002 13:42:54 -0500
Subject: LM for collagen in ovarian tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
I am looking at developmental stages of oogenesis in sharks and rays. For
part of this work I want to document stages using light microscopy,
particularly looking at distribution and amounts of collagen around the
oocytes. Samples are fixed in ~ 4% para/2%glut. I have tried Mallorys
triple (mordant HgCl), Pollocks (HgCl) and recently Gomori triple (Bouins).
I have yet to get any other colours than pinks and reds for the Mallory and
Gomori, the Pollocks dissolves my sections of the slides.
I have used JB4 & Technovite, sections ~ 2-3 um dried on glass slides at
70C for 20-30 minutes.
Any suggestions as to why the collagen is proving so elusive? It is there I
have in TEM`s.

Thanks Ian


Ian R. Davenport
Ph.D. candidate
Clemson University
Department of Biological Sciences
132 Long Hall
Clemson, SC 29634-0326
Phone no. 864-656-3598
Fax no. 864-656-0435




From daemon Thu Nov 14 13:51:27 2002



From: rcmoretz-at-att.net
Date: Thu, 14 Nov 2002 19:43:01 +0000
Subject: Re: Osmication - R-OTO technique

Contents Retrieved from Microscopy Listserver Archives
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I have a number of references to use of the OTO method that includes TEM. The
one caveat I remember (having primarily used the technique for SEM) is the
quality of the thiocarbohydrazide. One paper (and it was for SEM)recommended
washing the TCH to remove some impurities that could/would compromise the
results. I can't find my protocols right now (I'll look again later) but here
are some references that include TEM in the results.

Aoki, M. & Tavassoli, M. OTO method for preservation of actin filaments in
electron microscopy. J Histochem Cytochem 29:682-3, 1981

Vriend, R.A. & Geissinger, H.D. An improved direct intermicroscopic
(LM} SEM} TEM) correlative procedure for the examination of mammalian skeletal
muscle. J Microscopy 120:53-64, 1980

Geissinger, H.D. et al. Osmium-thiocarbohydrazide-osmium versus tannic acid-
osmium staining of skeletal muscle for scanning electron microscopy and
correlative microscopy. Trans Amer Microsc Soc 102:390-8. 1983

Birn, H., Christensen, E.I., & Nielsen, S. Kinetics of endocytosis in renal
proximal tubules studied with ruthenium red as membrane marker. Am J Physiol
264:F239-F250, 1993

Lemke, C. & Linss, W. The employment of the OTO-method for presentation of
cytoskeletal components in enucleating erythroblasts. Acta Histochem 33,
Suppl: 69-72, 1986

Miyai, K. et. al. Scanning electron microscopy of hepatic ultrastructure.
Secondary, backscattered, and transmitted electron imaging. Lab Invest 35:369-
376, 1976

Plus the Willingham paper, of course.

If/when I find my protocol (haven't done this for several years now, so
everything is filed real deep) and reference I will send the info.

Roger Moretz, Ph.D.
Dept of Toxicology
BI Pharmaceuticals
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
}
}
} Dear Sir:
} You are right, it is not easy to find references about the OTO method, but it
} works very well in transmission electronmicroscopy studies. It was a method used
} by people who studied intestinal absorption in fishes in the 70´s and 80´s as it
} was my case. I suggest that you look at the original paper of Seligman et al.,
} 1966, Journal of Cell Biology 30:424-432. I´ve used this method in my associate
} professor thesis and I was succesful at first try I just followed the recipe of
} the paper.
} My objective was to demonstrate quilomicra and lipid droplets in the enterocytes
} of an antarctic fish. This paper was not yet published.
}
}
} Prof. Dr. Francisco Javier Hernandez Blazquez
} Departament of Surgery - Sector of Anatomy
} Faculty of Veterinary Medicine and Animal Sciences
} University of São Paulo
} Av. Prof. Dr. Orlando Marques de Paiva, 87
} 05508-000 - São Paulo (SP) - Brazil
} Tel..55 (11) 3091 1374 Fax 55 (11) 3091 7805
} email: fjhblazq-at-usp.br
}
}
}
} At 11:09 12/11/02 -0600, Tom Phillips wrote:
}
} } I have just read Willingham & Rutherford (1984) J. Histochem Cytochm
} 32(4):455-460 paper on the use of ferrocyanide reduced osmium with the
} osmium-thiocarbohydrazide-osmium (OTO) technique of Seligman. I thought the
} conclusion that R-OTO gave a superior preservation and contrast of membranes in
} TEM was a good one based on their images. But when I did a Medline search for
} papers using either the OTO or R-OTO technique, the limited number of papers was
} essentially limited to SEM work. Perhaps the method is buried in papers and not
} coming up in a keyword search. Or maybe no one uses it or there are problems } with it?? Has any one used the R-OTO (or even OTO) technique with tissue
} biopsies (Willingham & Rutherford use cell cultures so it is tough to
} extrapolate the correct osmication times)? Are there hidden pitfalls and
} disadvantages to this technique? Any comments welcome. Tom
} }
} }
} } Thomas E. Phillips, PhD
} } Associate Professor of Biological Sciences
} } Director, Molecular Cytology Core
} } 3 Tucker Hall
} } University of Missouri
} } Columbia, MO 65211-7400
} }
} } 573-882-4712 (office)
} } 573-882-0123 (fax)
} } PhillipsT-at-missouri.edu
}
}
}


From daemon Thu Nov 14 14:36:15 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Thu, 14 Nov 2002 12:29:55 -0800
Subject: Re: Ask-A-Microscopist: statistically justify the number of TEM samples

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Friday, November 8, 2002, at 07:07 AM, by way of
MicroscopyListserver wrote:
}

} Name: ramki kalyanaraman
}
} Organization: Washington University in St. Louis
}
} Education: Graduate College
}
} Location: St. Louis, MO, USA
}
} Question: Is there a way to statistically justify the number of TEM
} samples that must be prepared to be confident. One answer to this may
} be on the basis of the feature size beining invesitgated. There are
} probably two extreme cases to base the answer upon: In one case we
} have numerous independent prior measurements of the sample properties
} while in the other we do not. If there are any good journal paper/Book
} chapter that answers this question, I would appreciate knowing about
} it.
} thank you

Dear Ramki,
Suppose you can describe the state of your images by the values of one
or more parameters, and that each parameter takes on a range of values
within your set of images. For parameters that do not vary
significantly from one image to the next, few images would be necessary
to have confidence, but for parameters whose value differs, you would
have to measure the distribution of values, requiring many images. At
the very least, you would need two images to have any idea whether a
parameter varied. Examples of the extremes are the number of lipid
layers in, say, outer mitochondrial membrane, or the number of faces on
an icosahedral virus, versus the volume of a cell or the density of
receptors on its surface. In the first cases, a few images combined
with prior knowledge would be enough, but in the second cases, many
more measurements would be necessary (although, if ten measurements
indicated that the parameters had a Gaussian distribution, you could
extract mean and SD values). Similar considerations for
non-quantitative parameters are less certain, but you would at least
have to show that independent preparations of your sample gave the same
results for the feature of interest. I seem to remember this
discussion happening previously on this list, so you may wish to look
through the archives.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Thu Nov 14 15:03:37 2002



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Thu, 14 Nov 2002 15:38:00 -0500
Subject: TEM Printing - Polycontrast Paper: Print Processor Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


November 14, 2002

Good Afternoon,
} From the volume of e-mail exchanged on the subject of polycontrast paper it
seems there are many that continue to make use of their darkrooms. We have
recently sold our enlarger and would also be happy to part with a Kodak
Dektomatic 65 Print Processor. If you're interested we are willing to sell
it for Cdn.$1000.00. Contact me off-line if you'd like more information.

Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com



From daemon Thu Nov 14 16:29:49 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 14 Nov 2002 17:22:01 -0500
Subject: Re: LM for collagen in ovarian tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Several thoughts come to mind:

1. How does glutaraldehyde fixation affect the stains you are using? Glut
fixation makes many tissues "overly" eosinophilic. Can you try Bouin's or even
buffered formalin?
2. Why use a trichrome if you are just looking for collagen? How about a
specific collagen (only) stain (van Gieson, picro-sirius red with polarized
light) to keep things simple.
3. Are you sure the stain you are using penetrates the plastic you are using?
Got any paraffin-embedded specimens?
4. Is the collagen you see in the TEM 'bundled' like type I and II or more like
the reticular fibers of type III?

ian davenport wrote:

} Hi
} I am looking at developmental stages of oogenesis in sharks and rays. For
} part of this work I want to document stages using light microscopy,
} particularly looking at distribution and amounts of collagen around the
} oocytes. Samples are fixed in ~ 4% para/2%glut. I have tried Mallorys
} triple (mordant HgCl), Pollocks (HgCl) and recently Gomori triple (Bouins).
} I have yet to get any other colours than pinks and reds for the Mallory and
} Gomori, the Pollocks dissolves my sections of the slides.
} I have used JB4 & Technovite, sections ~ 2-3 um dried on glass slides at
} 70C for 20-30 minutes.
} Any suggestions as to why the collagen is proving so elusive? It is there I
} have in TEM`s.
}
} Thanks Ian
}
} Ian R. Davenport
} Ph.D. candidate
} Clemson University
} Department of Biological Sciences
} 132 Long Hall
} Clemson, SC 29634-0326
} Phone no. 864-656-3598
} Fax no. 864-656-0435

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Nov 14 16:29:51 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 14 Nov 2002 17:23:22 -0500
Subject: TEM thin sectioning of coating layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Barbara Malony wrote:
==========================================================
Hi group - anybody out there working on layers of paint samples?
Looking to distinguish layers by wavelength. Apparently this party has
already used SEM and optic scopes without success. Guess density is to
close to distinguish layers. We have been told that a PanaCL could do
the trick - would appreciate any suggestions.
==========================================================
Generally speaking, paint layers are pigmented and each different layer has
a somewhat different pigment system, so on the basis of morphology alone,
different layers are easily discerned via diamond knife ultramicrotomed thin
sections and TEM. While the temptation to use LM and SEM is great because
of the apparent ease of sample preparation, the information contained by way
of the thin section TEM views is vastly superior to any other method (in my
humble opinion). I have seen many systems by which by LM adjacent layers
looked the same but by TEM, vastly different.

We have also found that multi-layers of the same unpigmented polymer, when
thin sectioned, for reasons I have never been able to explain to my
satisfaction, if the sections are thin enough, you can get some kind of
contrast at the interface between the various layers. So even when the
polymer composition is the same for adjacent layers, if the sectioning is
done without distortion (e.g. with diamond knives and at cryo temperatures)
you can resolve the different layers, measure their thicknesses, and observe
any contaminants that might be present between them. If one wants to
determine how many "passed" might have been made on a substrate that has had
applied multi-layers, this is the way to answer that kind of question.

Now this is not a spectroscopic kind of analysis (as originally indicated)
but then again, neither is SEM or LM which was also mentioned. Micro-FT/IR
in theory might work, but the spot size relative to the typical layers often
times is too large to obtain an unambiguous result. And with pigments being
present, the analysis gets even more complicated.

Chuck

Disclaimer: SPI Supplies and Structure Probe, Inc. perform this kind of TEM
analytical work as a contract research organization and also via our SPI
Supplies division, offer the diamond knives used for this kind of sectioning

From daemon Thu Nov 14 16:38:03 2002



From: O'Neil, Ed F ERDC-GSL-MS :      Ed.F.O'Neil-at-erdc.usace.army.mil (by way
Date: Thu, 14 Nov 2002 14:24:49 -0800
Subject: Re: SEM particle size measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A technique using image analysis for calculating pore size distribution in
hardened cement paste is described in "pore size distributions in hardened
cement paste by SEM image analysis", Sidney Diamond, and Mark Leeman,
Materials Research Society Symposium Proceedings, "Microstructure of
cement-based systems/Bonding and interfaces in cementitious materials" Nov
28-Dec 1 1994, published by Materials Research Society Pittsburg, PA c1995.
The article is based on imaging capillary pores on the size order of 1 to 10
um.

Ed O'Neil
Research Civil Engineer
Concrete and Materials Branch
Geotechnical and Structures Laboratory
US Army Engineer Research and Development Center
Vicksburg, MS 39180





-----Original Message-----
} From: Gareth Morgan [mailto:Gareth.Morgan-at-impi.ki.se]
Sent: Wednesday, November 13, 2002 12:44 AM
To: Erasmus, Willem (WJ); Microscopy-at-sparc5.microscopy.com


Hi

You will find a description of what you want in the book "Morphometry" by
Aherne WA and Dunnill MS, Pub. Arnold, ISBN 0 7131 4538 2. It is out of
print but Amazon are selling it second hand at

http://www.amazon.com/

I would be interested in knowing what other suggestions people come up with.


At 12:07 2002-11-12 +0200, Erasmus, Willem (WJ) wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Obs/NB New postal/visiting address from July 2002!

Med vänliga hälsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 5858 1038
Fax +46 728 3747

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Huddinge Universitetssjukhus, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.


From daemon Fri Nov 15 05:30:41 2002



From: Ezekiel E :      ezekiel2002-at-email.ro
Date: Fri, 15 Nov 2002 13:19:03 +0200
Subject: Please Stand As Next of Kin

Contents Retrieved from Microscopy Listserver Archives
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ECO BANK PLC
LAGOS - NIGERIA.
ezekiel2002-at-email.ro or ezekiel2002-at-popmail.com
Tel: 234 - 80 - 334 - 31659

VERY CONFIDENTIAL.

I am the manager of Foreign Account Department of the ECO Bank Plc. I am
writing you this letter to ask for yoursupport and co-operation to carry out
this transaction. We discovered and abandoned Sum of US$20Million (Twenty
Million U.S Dollars) in an account that belongto one of our foreign customer
who died along side his entire family in 21st of April 2000 in a plane crash in
lagos.Since this development, we have advertised for his next kin or any
closerelation to come forward to claim this money, but nobody came to applyfor
the claim.

To this effect, I and two other officials in my departmenthave decided to look
for a trusted foreign partner who can stand in as the next of kin to the
deceased and claim this money. We need a foreign partner to apply for the claim
because of the fact that the customerwas a foreigner. And we don't want this
money to go into the bank's treasure as unclaimed fund.Every document to affect
this process will emanate from our table and I will perfect every documents to
be in accordance with the banking law and guidelines so you have nothing to
worry about.

All we required from you is you to open a local bank account in your name here
in Lagos where the money is to be deposited,before onward transfer to your
designated account abroad. We have agreed that 30% of the money will be for
you, 10% for expenses incurredon the both side while 60% willbe for my
colleagues and me. If you are going to help me, indicate by replying thisletter
and putting in your bank particulars, private telephone and faxnumbers.


I await your immediate reply to enable us start this transaction in earnest.
Once I receive your reply, I will send you the text of application for
immediate application of claim.

Thanks for your anticipated assistance. You can replythrough my private e-mail
address:ezekiel2002-at-email.ro or ezekiel2002-at-popmail.com




Yours faithfully,

EZEKIEL




______________________________________________________________________
Do you want a free e-mail for life ? Get it at http://www.email.ro/



From daemon Fri Nov 15 08:22:34 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 15 Nov 2002 08:08:22 -0600
Subject: Re: LM for collagen in ovarian tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian,

If you don't get answers here, try Histonet:
histonet-at-pathology.swmed.edu

This list uses the same address for commands and posts: send
"subscribe", no quotes, correctly spelled in the *subject line*,
nothing in the body to subscribe. Correct spelling is essential,
otherwise the server thinks your command is a post and sends it to
the list.
You'd be amazed at how many people can't spell "subscribe" or "unsubscribe".
Then send your question to the same address.
There are several knowledgeable people on this list who do fish,
amongst other animals.

Phil

} Hi
} I am looking at developmental stages of oogenesis in sharks and
} rays. For part of this work I want to document stages using light
} microscopy, particularly looking at distribution and amounts of
} collagen around the oocytes. Samples are fixed in ~ 4% para/2%glut.
} I have tried Mallorys triple (mordant HgCl), Pollocks (HgCl) and
} recently Gomori triple (Bouins). I have yet to get any other colours
} than pinks and reds for the Mallory and Gomori, the Pollocks
} dissolves my sections of the slides.
} I have used JB4 & Technovite, sections ~ 2-3 um dried on glass
} slides at 70C for 20-30 minutes.
} Any suggestions as to why the collagen is proving so elusive? It is
} there I have in TEM`s.
}
} Thanks Ian
}
}
} Ian R. Davenport
} Ph.D. candidate
} Clemson University
} Department of Biological Sciences
} 132 Long Hall
} Clemson, SC 29634-0326
} Phone no. 864-656-3598
} Fax no. 864-656-0435

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Nov 15 09:26:45 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 15 Nov 2002 07:03:55 -0800
Subject: Re: paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Hi group - anybody out there working on layers of paint samples?
} Looking to distinguish layers by wavelength. Apparently this party has
} already used SEM and optic scopes without success. Guess density is to
} close to distinguish layers. We have been told that a PanaCL could do
} the trick - would appreciate any suggestions.
} Thanks
} Barb -

Have you considered microtomy of embedded samples? It isn't difficult.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Nov 15 09:37:18 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Fri, 15 Nov 2002 10:29:31 -0500
Subject: LM for collagen in ovarian tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian:

You definitely will have a problem with light microscopy stains if you use
glutaraldehyde in your fixative. Everything becomes extremely eosinophilic
when staining with Hematoxylin and Eosin, therefore other stains are also
affected.

If you are trying to take samples only for LM workup then change to 10%
buffered formalin (equivalent is 4.0% paraformaldehyde pH 7.4). But if you
need to use the same fixed tissue for both LM and EM, try reducing the
amount of glut to 0.5-1.0% in your fixation. However since collagen is
really very sturdy it would look very good at the EM level with a
paraformaldehyde fixation alone.

Also, since there are many different types of collagens, you could do an
immunohistochemistry procedure to localize in the tissue.

Karen Bentley

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954




-----Original Message-----
} From: ian davenport [mailto:iand-at-CLEMSON.EDU]
Sent: Thursday, November 14, 2002 1:43 PM
To: Microscopy-at-sparc5.microscopy.com


Hi
I am looking at developmental stages of oogenesis in sharks and rays. For
part of this work I want to document stages using light microscopy,
particularly looking at distribution and amounts of collagen around the
oocytes. Samples are fixed in ~ 4% para/2%glut. I have tried Mallorys
triple (mordant HgCl), Pollocks (HgCl) and recently Gomori triple (Bouins).
I have yet to get any other colours than pinks and reds for the Mallory and
Gomori, the Pollocks dissolves my sections of the slides.
I have used JB4 & Technovite, sections ~ 2-3 um dried on glass slides at
70C for 20-30 minutes.
Any suggestions as to why the collagen is proving so elusive? It is there I
have in TEM`s.

Thanks Ian


Ian R. Davenport
Ph.D. candidate
Clemson University
Department of Biological Sciences
132 Long Hall
Clemson, SC 29634-0326
Phone no. 864-656-3598
Fax no. 864-656-0435




From daemon Fri Nov 15 13:49:13 2002



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 15 Nov 2002 19:36:40 -0000
Subject: Re: Help on TEM replica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fang Mei,

I'd like to reinforce (sorry about the pun) Mary Mager's suggestion of
carbon as a replicating material. The other day, for the first time
ever, I had hands-on experience with an AFM, and now I understand it
much better.

The two things that carbon has going for it are:

(1) it forms a very faithful replica of the surface it's applied to,
down to very fine detail. Cellulose acetate and its relatives can
sometimes go "liquid crystally" and introduce structures of the order
of tens of nm that simply aren't there in the original.

(2) it is very mechanically robust.

I'd like to tell you about a technique, and I hope you won't mind the
length, but the details are relevant.

Philip H Geil of University of Illinois at Urbana-Champaign developed
in the early 1960's a direct replication technique for polymers that
did not involve intermediate cellulose acetate or similar replicas.
To give contrast he would first put down a very thin coat of heavy
metal (Pt/Pd?), but this you would NOT need or want for AFM. Then he
applied carbon to this, which he would back with polyacrylic acid by
applying PAA solution and drying gently to give a hard bead. He could
then flick off the bead and extract the carbon-shadowed replica by
dissolving the PAA in water.

With our etched polymers it was not so easy applying this technique,
because the shadowing metal keys in to the etched surface and grips
like fury when you try to pull the bead off. One can often pull it
off etched polypropylene OK, but with polyethylene its grips tighter
than a leech. However, without the shadowing metal, the carbon comes
away much easier.

Therefore if you back the carbon with something hard, you should be
able to pull it off intact. I think an epoxy resin might do the
trick. That way you will get a faithful replica both of the finest
details and also the large-scale structure. My recently retired boss
(Prof. D.C.Bassett) very long ago backed carbon replicas with copper
by electroplating, removed them, and then dissolved the copper off
with dilute nitric acid. But for AFM the hard backing, whether of
resin or metal, can be kept and should make it easier to handle in the
AFM.

All the best with your efforts,

+-----------------------------------------+
Free Ian Stillman - deaf, disabled and wrongly convicted
Info {http://www.ianstillman.fsnet.co.uk}
+-----------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+-----------------------------------------+
Phone:
{direct line +44 (0) 118 9318572
{University internal extension 7867
Fax: +44 (0) 118 9750203
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+-----------------------------------------+



From daemon Fri Nov 15 14:12:15 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 15 Nov 2002 12:04:01 -0800
Subject: Address needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know Fred Lightfoot's current address?

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Nov 15 14:28:39 2002



From: Paul.Nolan-at-alcan.com
Date: Fri, 15 Nov 2002 15:20:02 -0500
Subject: Re: paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



You can microtome the face of your sample in cross section (no need to
embed), coat and look at them in the SEM.
I do this with multilayer polymers all the time.
its fast and easy.
Make sure you microtome at an angle with regard to the layer direction so
as not to confuse a scratch mark with a layer. I use 45 ° angle

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



Caroline
Schooley To: Barbara Maloney {maloneyb-at-fiu.edu}
{schooley-at-mcn.or cc: Microscopy-at-sparc5.microscopy.com
g} Subject: Re: paint samples

11/15/2002 10:03
AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


}
} Hi group - anybody out there working on layers of paint samples?
} Looking to distinguish layers by wavelength. Apparently this party has
} already used SEM and optic scopes without success. Guess density is to
} close to distinguish layers. We have been told that a PanaCL could do
} the trick - would appreciate any suggestions.
} Thanks
} Barb -

Have you considered microtomy of embedded samples? It isn't difficult.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html









From daemon Sat Nov 16 00:28:36 2002



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Sat, 16 Nov 2002 17:15:58 +1100
Subject: unusual messages in microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I will be delighted to know if are these messengers proposing the business
in Zimbabwe, Nigeria etc, are members of this group and microscopists?

Keep care and be of good cheer

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
Coleoptera - Australia, Tenebrionidae of the World (incl. Lagriinae,
Alleculinae)


websites:
http://www.coleoptera.org. and
http://www.egroups.com/group/coleoptera

University of Sydney
The Wentworth Bldg., B 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: ricardo-at-ans.com.au
vratislav-at-bigfoot.com
ICQ: 13610107

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).



From daemon Sat Nov 16 02:30:27 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 16 Nov 2002 03:21:09 -0500
Subject: TEM replication of polymer surfaces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Robert H.Olley wrote:
============================================================
Fang Mei,

I'd like to reinforce (sorry about the pun) Mary Mager's suggestion of
carbon as a replicating material. The other day, for the first time ever, I
had hands-on experience with an AFM, and now I understand it much better.

The two things that carbon has going for it are:

(1) it forms a very faithful replica of the surface it's applied to, down to
very fine detail. Cellulose acetate and its relatives can sometimes go
"liquid crystally" and introduce structures of the order of tens of nm that
simply aren't there in the original.

(2) it is very mechanically robust.

I'd like to tell you about a technique, and I hope you won't mind the length
, but the details are relevant.

Philip H Geil of University of Illinois at Urbana-Champaign developed in the
early 1960's a direct replication technique for polymers that did not
involve intermediate cellulose acetate or similar replicas. To give
contrast he would first put down a very thin coat of heavy metal (Pt/Pd?),
but this you would NOT need or want for AFM. Then he applied carbon to this
, which he would back with polyacrylic acid by applying PAA solution and
drying gently to give a hard bead. He could then flick off the bead and
extract the carbon-shadowed replica by dissolving the PAA in water.

With our etched polymers it was not so easy applying this technique, because
the shadowing metal keys in to the etched surface and grips like fury when
you try to pull the bead off. One can often pull it off etched
polypropylene OK, but with polyethylene its grips tighter than a leech.
However, without the shadowing metal, the carbon comes away much easier.

Therefore if you back the carbon with something hard, you should be able to
pull it off intact. I think an epoxy resin might do the trick. That way
you will get a faithful replica both of the finest details and also the
large-scale structure. My recently retired boss (Prof. D.C.Bassett) very
long ago backed carbon replicas with copper by electroplating, removed them,
and then dissolved the copper off with dilute nitric acid. But for AFM the
hard backing, whether of resin or metal, can be kept and should make it
easier to handle in the AFM.
================================================================
You are quite correct in that Prof. Geil was a real innovator in those days
(as I am sure he still is (now) at the University of Illinois). I did not
realize your "retired boss" was Prof. Bassett, as in those days (mid-1960's)
, the famous "three-some" of polymer replication studies, were, in addition
to Geil and Bassett, (now also retired) Prof. Andrew Keller of Bristol. The
book "Polymer Single Crystals" published by Prof. Geil in 1963 (still a
bible but I am sure it is out of print) contained huge numbers of the
original micrographs of all three of the pioneers. I was Professor Geil's
first graduate student in those days (I came **after** he did his previously
mentioned pioneering work) but I can speak at least to some degree from
first hand knowledge of the technique. Actually, Prof. Geil's technique was
not developed at the University of Illinois but at his first position at the
DuPont Experimental Station in Wilmington, DE and later at the Camille
Dreyfus Laboratories in North Carolina before coming to Case in the fall of
1963.

The technique as was taught to me involved pure platinum wire which was
evaporated from the end of a carbon rod using the "platinum bead" technique,
which was discussed previously on this listserver but which I will repeat if
anyone needs it again. This is what did the "shadowing" of the polymer
surface to be replicated. It worked both on etched and non-etched surfaces.
The replica was a combination of Pt and carbon and fairly small grain size
(well under 1 nm for the Pt) but which was quite adequate considering the
resolution of the TEMs in those days.

The PAA came out of a special "cache" that Prof. Geil had selected from a
variety of different candidate PAA samples. At the time he was at Case
Institute (now Case Western Reserve University) and as a going-away present
in 1967, I was given a small bottle to use in my own continuing work. I
have since tried other PAAs and have not gotten the same results. I
safeguard the small amount of the "special" PAA and use it sparingly. So
the first point is, PAA is not "generic" and clearly the MW must be
important. In those days, a 1% solution was used. When the technique is
practiced, what starts out as a "bead" ends up 24 hours later as a hard
brittle "pancake" since the water has evaporated leaving the polymer film
behind. I had always found that the edge of a scalpel or razor blade at the
edge of the pancake would cause it to "pop" right off (in later years I
found that to be true even on rough fracture surfaces). There are some
polymers, such as PTFE that easily fibrillate, so there are modifications of
the technique for such occasions.

At this point, after the "pancake" has been "popped off" of the surface to
be replicated, the black side which is the replica of platinum/carbon is
then "backed" with another layer of carbon. I preferred a 90° coating angle
(e.g. directly underneath the carbon rods) but others preferred some other
angle. But without this carbon "backing layer", when the PAA pancake was
dissolved in water, the replica would generally "break up" on the water
surface (from surface tension effects). But with the carbon backing layer,
this did not happen. This was part of Prof. Geil's original protocol, as
he saw the need for such a backing layer from the beginning.

In the case of polyolefins and non-PTFE surfaces, if material was being
pulled off (which would cloud the eventual examination of the replica), a
variation I have used successfully, is to use a backing layer of silicon
monoxide/dioxide which is evaporated from a tungsten basket and silicon
monoxide. Then that surface can be exposed to an oxygen plasma for
literally just a few seconds to get rid of the polymer debris. The plasma
would harm a carbon layer but not the SiO/SiO2 layer, which also serves as a
backing layer for the replica, just as the carbon would have otherwise done.
But this is generally not needed and the reason might be due to the
special characteristics of the magic PAA.

Well, if Bob had to apologize for length, I guess I should do that times two
. But this is the technique we would still use today in our own laboratory
when we have the need to do careful replication on a polymer surface for TEM

From daemon Sat Nov 16 12:57:56 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 16 Nov 2002 15:30:55 -0800
Subject: Re: unusual messages in microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barb,
We work routinely - as well as many conservation science labs - on
multilayered paint samples from paintings and other painted works of art.
We use mostly LM of embedded and polished minute flakes of painting layers
as we have to examine colours of subsequent layers - and pigmented layers
are recognised easily of course. The binding media, however are not
distinguishable. What we can do is to use a reactive dyes which may dye a
layer of certain composition (for instance Amido Black for proteins) or, now
more and more popular, UV microscopy which seems to be very promising.
SEM is of great importance in recognising the pigments' composition but, as
it is B&W, it do not always allow to recognise layers if they have, for
instance, the same chemical composition but different morphology. I have
to add that old paints are usually brittle and crush easily, thus the
microtoming is difficult and rarely successful.

Pawel

dr Pawel Karaszkiewicz
Conservation Science Lab
Academy of Fine Arts
Krakow, Poland


I hope that
----- Original Message -----
} From: Barbara Maloney {maloneyb-at-fiu.edu}
To: 'microscopy-at-msa.microscopy.com' {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, November 14, 2002 4:25 PM


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Richard -

"Keep care" is quite appropriate. No, they aren't microscopists (have you
ever heard of one with that kind of money?). It's a well-known (in the
U.S., anyway) fraud that actually captures an occasional gullible victim.
They've stolen MANY address lists.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sat Nov 16 19:20:19 2002



From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Sat, 16 Nov 2002 19:11:13 -0600
Subject: Fwd: Re: unusual messages in microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I get at least four of these a week. Often more.
Joiner Cartwright, Jr.
Houston, Texas



} X-Sender: schooley-at-mail.mcn.org
} Date: Sat, 16 Nov 2002 15:30:55 -0800
} To: "Vr. Richard Bejsak-Colloredo-Mansfeld" {ricardo-at-ans.com.au}
} From: Caroline Schooley {schooley-at-mcn.org}
} Subject: Re: unusual messages in microscopy
} Cc: Microscopy-at-sparc5.microscopy.com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Nov 17 05:10:13 2002



From: Tianli Zhang :      zhongshunchem-at-21cn.com
Date: Sun, 17 Nov 2002 18:59:56 +0800
Subject: supply FOOD ADDITIVES-sodium citrate etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Purchasing Manager,
Please allow me to introduce our company as a biggest manufacturer of food additives products
in China. Our main products are SODIUM CITRATE, CALCIUM CITRATE, POTASSIUM CITRATE,
SODIUM MALATE etc. All products have been certified by ISO9002 AND ISO14001. If some items
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More information will be sent to you as soon as receiving your inquiry.
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Looking forward to hearing from you soon with much interests.
Best regards!

Tianli Zhang / Sales Manager
Zhongshun Sci. & Tech. Devel. Co., Ltd.
Tel 86 533 5859603 Fax 86 533 5810955
Personal email:ztl-at-zhongshun.com


From daemon Sun Nov 17 05:42:35 2002



From: Tran Quang Huy :      tranquanghuy78-at-yahoo.com
Date: Sun, 17 Nov 2002 03:34:56 -0800 (PST)
Subject: How to get EM diff photos fo asbestos?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,
Recently, I have been using TEM and SEM to analyse and
determine some asbestos samples (by using EM
diffration method).But I have no experience in this
field. Could you show me to get standard EM
diffraction photos of asbestos (crocidolite,
anthophylitte, amosite, actinolite,
trimosite,chrysotile)
Thank you in advance

Tran Quang Huy

__________________________________________________
Do you Yahoo!?
Yahoo! Web Hosting - Let the expert host your site
http://webhosting.yahoo.com


From daemon Sun Nov 17 11:28:03 2002



From: Gordon Nord :      gnord-at-mindspring.com
Date: Sun, 17 Nov 2002 12:09:57 -0500
Subject: Re: How to get EM diff photos fo asbestos?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tran,

You are in luck. All the diffraction patterns you need are published in
J. L. Hutchison, M. C. Irusteta and E. J. W. Whittaker, 1975,
High-resolution electron microsccopy and diffraction studies of fibrous
amphiboles. Acta Crystallographica, vol. A31, part 6, November,
p.974-801. Available online at {http://www.iucr.org} .

Get the original out of your library - a photocopy doesn't show the
figures well.

Otherwise the best way to positively identify your particles is by
calculating the patterns.
Pictures of high index zone axes are nice but they don't help you get
there.

Start with known standards so you can familiarize yourself with the
monoclinic (C2/m) and orthorhombic (Pnma) patterns.
Start with anthophyllite.

Watch out for twinning, multiple-chain defects, exsolved phases and
alteration.
Have fun and book plenty of TEM time.

Dr. Gordon Nord
Senior Scientist
Environmental Sciences Laboratory
Brooklyn College
Brooklyn NY 11210



On Sunday, November 17, 2002, at 06:34 AM, Tran Quang Huy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Microscopists,
} Recently, I have been using TEM and SEM to analyse and
} determine some asbestos samples (by using EM
} diffration method).But I have no experience in this
} field. Could you show me to get standard EM
} diffraction photos of asbestos (crocidolite,
} anthophylitte, amosite, actinolite,
} trimosite,chrysotile)
} Thank you in advance
}
} Tran Quang Huy
}
} __________________________________________________
} Do you Yahoo!?
} Yahoo! Web Hosting - Let the expert host your site
} http://webhosting.yahoo.com
}
}



From daemon Sun Nov 17 13:24:41 2002



From: sstouden-at-thelinks.com
Date: Sun, 17 Nov 2002 13:11:43 -0600 (CST)
Subject: Re: unusual messages in microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



but I used to get thesse same messages or similiar from the place as long
as as 1998 and before that by mail.

The State Department has a long list of problems associated with the
certain quote deals unquote or "needs" or opportunities; but it would seem
possible to trace them, since the mail server probably has a log file?

and the log file could be used trace the message back to the IP and match
the ip with the user. a few minutes to do this, unless of course it is
one of those Micro whomever servers.. then it might take a few
blue screens longer.

either way, should be possibleto discover the lister if they came threw
the list, if external to the list, then you need to ask your isp to trace
those messages out,, keep the email header details.

believe the FBI and othersecurity agencies might also

On Sat, 16 Nov 2002, Caroline Schooley wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
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} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I will be delighted to know if are these messengers proposing the business
} } in Zimbabwe, Nigeria etc, are members of this group and microscopists?
} }
} } Keep care and be of good cheer
} }
} } Richard Bejsak-Colloredo-Mansfeld
}
} Richard -
}
} "Keep care" is quite appropriate. No, they aren't microscopists (have you
} ever heard of one with that kind of money?). It's a well-known (in the
} U.S., anyway) fraud that actually captures an occasional gullible victim.
} They've stolen MANY address lists.
}
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
} Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
}
}
}



From daemon Sun Nov 17 13:47:31 2002



From: Ralph Common :      Ralph.Common-at-ht.msu.edu
Date: Sun, 17 Nov 2002 16:42:35 -0500
Subject: Re: LM for collagen in ovarian tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I've just been to a lecture by a guy who claims that laser Raman microscopy is the
way to go, apparently has ALL of the required virtues: spacial resolution, specificity,
works for organics.

cheers

rtch




Date sent: Fri, 15 Nov 2002 07:03:55 -0800
To: Barbara Maloney {maloneyb-at-fiu.edu}
} From: Caroline Schooley {schooley-at-mcn.org}



Excellent staining of collagen can be achieved on simithin sections of
tissue embedded in Epon type resins. I use a formulation of
Araldite:Poly/Bed 812:DDSA in the proportion 4:5:12. Stain the sections in
a mixture of 9 parts 1% toluidine blue (in 1% sodium borate) and 1 part of
1% aqueous Basic Fuchsin. The T-blue and Basic Fuchsin should be combined
just before use. Stain on a hotplate at 100 degrees C. After rinsing, the
slides should be placed back on the hotplate, destained briefly in alcohol,
and mounted with a drop of the same resin used for embedding. Nuclei will
be dark blue or purple, cell contents will be pale blue to pink, and
collagen will be dark red. I have tried this with cow ovaries, and the
differentiation of collagen is excellent. I don't know if the various forms
of collagen stain equally well. The same blocks can of course later be used
for TEM.

Ralph Common
Division of Human Pathology
Michigan State University





} Hi
} I am looking at developmental stages of oogenesis in sharks and
} rays. For part of this work I want to document stages using light
} microscopy, particularly looking at distribution and amounts of
} collagen around the oocytes. Samples are fixed in ~ 4% para/2%glut.
} I have tried Mallorys triple (mordant HgCl), Pollocks (HgCl) and
} recently Gomori triple (Bouins). I have yet to get any other colours
} than pinks and reds for the Mallory and Gomori, the Pollocks
} dissolves my sections of the slides.
} I have used JB4 & Technovite, sections ~ 2-3 um dried on glass
} slides at 70C for 20-30 minutes.
} Any suggestions as to why the collagen is proving so elusive? It is
} there I have in TEM`s.
}
} Thanks Ian
}
}
} Ian R. Davenport
} Ph.D. candidate
} Clemson University
} Department of Biological Sciences
} 132 Long Hall
} Clemson, SC 29634-0326
} Phone no. 864-656-3598
} Fax no. 864-656-0435

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)




From daemon Sun Nov 17 16:16:13 2002



From: eld26-at-cornell.edu
Date: Sun, 17 Nov 2002 17:08:42 -0500 (EST)
Subject: paint samples & Raman

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barbara,

A combined Raman+confocal system might be best if you have access to one.
The confocal would save you from having to microtome your specimens to
look at each layer.

Eve


-----------------------------
Eve Donnelly
Experimental Biomechanics Lab
Cornell University
130 Upson Hall
Ithaca, NY 14853
607.255-3582
fax 607.255.1222



From daemon Mon Nov 18 05:18:33 2002



From: Francisco J. Hernandez Blazquez :      fjhblazq-at-usp.br
Date: Mon, 18 Nov 2002 09:15:29 -0200
Subject: Re: LM for collagen in ovarian tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Stains that work in collagen bundles usually have a large molecular weight.
It is ok if your material is embedded in paraffin, but you have a problem
if you have methacrylate-embedded tissue, because the mesh of the plastic
resin is not large enough to allow the stain molecules permeate into the
tissue.
I usually stains at 60 oC for 1 hour for methacrylate sections.
Picro-sirius with haematoxilin as counterstaining works at this
temperature, but you need to use a picric acid based-fixative because the
picric acid acts as a mordant to the sirius red stain.
You may try to make a post-fixation in saturated picric acid if your
material was formerly fixed by GA, but I never tried it.
Good luck!

Prof. Dr. Francisco Javier Hernandez Blazquez
Departament of Surgery - Sector of Anatomy
Faculty of Veterinary Medicine and Animal Sciences
University of São Paulo
Av. Prof. Dr. Orlando Marques de Paiva, 87
05508-000 - São Paulo (SP) - Brazil
Tel..55 (11) 3091 1374 Fax 55 (11) 3091 7805
email: fjhblazq-at-usp.br

At 13:42 14/11/02 -0500, ian davenport wrote:
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Nov 18 06:59:52 2002



From: Heike Gabrisch :      hgabrisc-at-uno.edu
Date: Mon, 18 Nov 2002 06:54:39 -0600
Subject: desktop microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi,

Does anybody know a vendor of Desktop Microscopist?
(Laguna Labs/ James Stanley are not responding)

Heike




From daemon Mon Nov 18 08:03:33 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 18 Nov 2002 05:54:55 -0800 (PST)
Subject: Re: paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barb,

I've looked at many paint samples in cross section
using metallographic methods. This was done for the
automotive industry. Primer, colorcoat, and clearcoat
were all distinguishable on the metallograph.

Stu Smalinskas
Senior Metallurgist
SKF USA
Plymouth, Michigan

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Society of America

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From daemon Mon Nov 18 08:10:47 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 18 Nov 2002 06:04:17 -0800 (PST)
Subject: Re: unusual messages in microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This "business" made front page news in the Detroit
Newspapers this past summer. They've have duped a
number of people, some here in the Detroit area. It
doesn't stop with e-mail. They've actually met up
with a number of gullible people, arranged meetings in
Europe, and continued with their charade until they
milked nearly $100,000 dollars from their prey.

Stu Smalinskas
SKF USA
Plymouth, Michigan

-------------------------------------------------------
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Society of America

Richard -

__________________________________________________
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From daemon Mon Nov 18 09:56:26 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 18 Nov 2002 08:40:36 -0700
Subject: Re: unusual messages in microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am getting several of these messages per day. These people probably
"harvest" email addresses and other addresses from list servers and such. If
it wasn't Spam and had the potential to hurt some people (although none from
this list, I hope), I would even consider them funny. Getting email from "
COL.EMMANUEL KOROMAH of the Democratic Republic of Congo I am one of close
aids to the former president of Congo Democratic LAURENT KABILA of blessed
memory, may his soul rest in peace" is certainly an honor!

For those of the listers who want to do something about these mailings,
there is a web site you can go to and read about the scam:
http://home.rica.net/alphae/419coal/. There is supposedly also a Task Force
in DC that will investigate. Their email is on the web site. However, I
don't think they will do anything unless someone lost money. I got one of
those emails from South Africa and send an email to the South African
police. They even responded that they would follow-up.

Mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "sstouden-at-thelinks.com"-at-sparc5.microscopy.com
[mailto:"sstouden-at-thelinks.com"-at-sparc5.microscopy.com]
Sent: Sunday, November 17, 2002 12:12 PM
To: Caroline Schooley
Cc: Vr. Richard Bejsak-Colloredo-Mansfeld;
Microscopy-at-sparc5.microscopy.com



but I used to get thesse same messages or similiar from the place as long
as as 1998 and before that by mail.

The State Department has a long list of problems associated with the
certain quote deals unquote or "needs" or opportunities; but it would seem
possible to trace them, since the mail server probably has a log file?

and the log file could be used trace the message back to the IP and match
the ip with the user. a few minutes to do this, unless of course it is
one of those Micro whomever servers.. then it might take a few
blue screens longer.

either way, should be possibleto discover the lister if they came threw
the list, if external to the list, then you need to ask your isp to trace
those messages out,, keep the email header details.

believe the FBI and othersecurity agencies might also

On Sat, 16 Nov 2002, Caroline Schooley wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I will be delighted to know if are these messengers proposing the
business
} } in Zimbabwe, Nigeria etc, are members of this group and microscopists?
} }
} } Keep care and be of good cheer
} }
} } Richard Bejsak-Colloredo-Mansfeld
}
} Richard -
}
} "Keep care" is quite appropriate. No, they aren't microscopists (have you
} ever heard of one with that kind of money?). It's a well-known (in the
} U.S., anyway) fraud that actually captures an occasional gullible victim.
} They've stolen MANY address lists.
}
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
} Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
}
}
}



From daemon Mon Nov 18 10:21:26 2002



From: ROSSCAC-at-aol.com
Date: Mon, 18 Nov 2002 11:14:07 EST
Subject: SEM fresh tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good day to all on the listserver,
I am looking for some information about doing SEM on fresh tissue. Is
someone out there doing this? Would confocal work better?
Thanks in advance,
Connie Cummings
email:rosscac-at-aol.com


From daemon Mon Nov 18 11:06:35 2002



From: Andrew Werner :      werner-at-rosharon.oilfield.slb.com
Date: Mon, 18 Nov 2002 10:42:45 -0600
Subject: request for opinions on EDS system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Apologies if this is a double post - got rejected first time.

Is anyone here familiar with a company called IXRF and their EDS / digital
image capture system? They are on the web at www.ixrfsystems.com - their
customer base seems to include a number of well-known companies.

The attraction is that they are based in the same city (Houston) as our lab
- but that is a very poor reason to choose an instrument. I am running a
PGT IMIX system on an ISI DS130 SEM and whenever the PGT system crashes, it
costs quite a lot to get it fixed - and much of the cost is travel
expense. So does anyone have an opinion of IXRF or any advice or
suggestions? Thanks in advance for your guidance.

Regards,
Andrew T. Werner
Chief Metallurgist
Shaped Charge Research - Metallurgy Laboratory
Schlumberger Reservoir Completions Technology Center
14910 Airline Road, Rosharon, TX 77583-1590
Voice (281) 285-5272 Fax (281) 285-5273



From daemon Mon Nov 18 11:24:58 2002



From: Mike Mizell :      mizell-at-emispec.com
Date: Mon, 18 Nov 2002 10:12:05 -0700
Subject: desktop microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Heike;
Jim Stanley is working with us here at Emispec.
You can contact him at JStanley-at-emispec.com

**********************************************************************
Michael K. Mizell Phone: 480-894-6443 ext 28
Manager of Sales and Marketing Fax: 480-894-6458
Emispec Systems, Inc. Cell: 602-743-2169
2050 S. Cottonwood Dr. Email: mizell-at-emispec.com
Tempe, AZ 85282
**********************************************************************
Please visit Emispec's NEW website www.emispec.com





From daemon Mon Nov 18 11:34:50 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 18 Nov 2002 09:29:24 -0800
Subject: Contact info for Mike Lamvik

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,
I seem to have lost track of Mike Lamvik, and the MSA Directory gave
no matches to his name. Does anyone have his contact info? TIA.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Mon Nov 18 12:31:43 2002



From: Mike Haugh :      mhaugh-at-resolve3d.com
Date: Mon, 18 Nov 2002 10:12:15 -0800
Subject: LM Using a Microtome to Cut Hard Materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Listers,

I am looking for advice about using a microtome to cut hard materials such
as brass, nickel, steel, stone, silicon, etc. I have had some successes,
many failures and nothing satisfactory. Up to now the embedding material
used is a modified Spurr. The microtome is part of a home made automated
system that uses stepper motors and motion control software. The sample is
embedded in a 2.5cm long block with an 8mm square cross section. This block
is held in a special clamp built into the microtome system. We image the
block face using a light microscope /camera system; the cut slice is
irrelevant. Imaging is done using either background fluorescence from the
Spur or reflection from the object with the Spurr as a dark background.
The cut/image sequence is done hundreds of times to obtain a 3-D image. I
have used diamond knives (45 degree), stainless steel and home made stellite
and tungsten carbide knives. Only the diamond has given reasonable results.
I generally cut .2 to .5 micrometers for these hard materials. Problems
include knife damage, excessive washboard, chipping the sample, distorting
the sample, etc. Up to now there has been no lubricant because that
requires significant modification to the system. I will modify and test
lubricants in the near term.

Any advice regarding embedding materials, knives, lubricants, etc. would be
greatly appreciated. Are there any references for cutting hard materials?
I would be glad to share my experiences.

Mike Haugh


Michael Haugh - Director of Operations
Resolution - http://www.resolve3d.com
530 Tamal Plaza, Corte Madera, CA 94925
Office:415/945-7360 x13 FAX:415/927-4495 Cell:415/987-9929
Email: mhaugh-at-resolve3d.com



From daemon Mon Nov 18 12:44:22 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 18 Nov 2002 13:37:21 -0500
Subject: How to get EM diff photos fo asbestos?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can obtain almost any standard text on electron microscopy to learn how to set up a TEM properly for selected area diffraction analysis. I recommend the Williams and Carter book from Plenum. You should have a diffraction standard like evaporated gold on a grid to calibrate you camera constant. You then use the camera constant that you find with the standard and using IDENTICAL conditions, record your unknowns, and find the d-spacings. For asbestos analysis, there are standard procedures that you can get from the US government (OSHA). I don't know whether they are on the internet or not.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Tran Quang Huy [mailto:tranquanghuy78-at-yahoo.com]
Sent: Sunday, November 17, 2002 6:35 AM
To: Microscopy-at-sparc5.microscopy.com


Dear Microscopists,
Recently, I have been using TEM and SEM to analyse and
determine some asbestos samples (by using EM
diffration method).But I have no experience in this
field. Could you show me to get standard EM
diffraction photos of asbestos (crocidolite,
anthophylitte, amosite, actinolite,
trimosite,chrysotile)
Thank you in advance

Tran Quang Huy

__________________________________________________
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Yahoo! Web Hosting - Let the expert host your site
http://webhosting.yahoo.com


From daemon Mon Nov 18 13:03:10 2002



From: saram-at-duke.edu
Date: Mon, 18 Nov 2002 13:52:23 -0500 (EST)
Subject: Re: SEM fresh tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The instrumentation will depend on the answer you need. Confocal is
certainly an option, but you might look into ESEM (the environmental SEM
now marketed by FEICO, or FEI-Philips, or whatever they're calling
themselves these days--they've just merged with somebody else). Confocal
will require some sort of staining, but can see layers under the surface.
ESEM is SEM at ambient pressure--and hence, can be done on wet tissue.

Sara Miller



On Mon, 18 Nov 2002 ROSSCAC-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Good day to all on the listserver,
} I am looking for some information about doing SEM on fresh tissue. Is
} someone out there doing this? Would confocal work better?
} Thanks in advance,
} Connie Cummings
} email:rosscac-at-aol.com
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Mon Nov 18 16:34:22 2002



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Mon, 18 Nov 2002 16:20:56 -0600
Subject: glycerol effect on fixed tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anybody know if 5-10% glycerol in buffer would have a negative
(morphological) effect on paraformaldehye or
paraformaldehyde/glutaraldehyde fixed tissues? Thanks

Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From daemon Mon Nov 18 17:15:00 2002



From: Norman C Miller :      Norman_C_Miller-at-raytheon.com (by way of
Date: Mon, 18 Nov 2002 17:08:55 -0600
Subject: Kevex 8000 EDS system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

We have a working Kevex 8000 EDS analyzer we no longer need. We also have a
Kevex silicon detector, horizontal mount, that is six years old, that we do
not need. Finally, we have an EDAX detector horizontal mount that we do not
need either. If you are interested, drop me an email.

N. Carl Miller


From daemon Mon Nov 18 17:36:45 2002



From: P. Geil :      geil-at-uiuc.edu
Date: Mon, 18 Nov 2002 17:27:51 -0600
Subject: One stage polymer surface replication

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With several messages discussing the replication technique we
use, I felt it time to participate and clarify several items. As
discussed in Polymer Single Crystals, the basic technique was
originally developed by Bob Scott in the Textile Fibers Dept. at
DuPont, sometime prior to 1957. At that time he was using Cr as the
shadowing material, stripping it from the surface with a dried drop
(of ca. EM grid diameter) of a 3% PAA water solution. (My
recollection is that we used Goodrite K714, a 30 % solution from
Goodrich that we diluted). This was then gotten onto a Formvar
substrate through a lengthy process in which the PAA-Cr sandwich was
backed on the Cr side with polystyrene, the PAA dissolved by floating
on water, the Cr-PS sandwich picked up on a Formvar coated grid and
the PS dissolved by rinsing. The PS was used to prevent swelling of
the PAA layer and breakup of the Cr during dissolution. An
improvement was based on the use of C substrates and Frank Anderson
(Chemstrand) informing me of a method of Pt/C shadowing. In Frank's
technique, which I don't know if he developed, several turns of
0.008" Pt wire are wrapped around a sharpened C rod tip, heated in
air while mounted in the evaporator to form a bead, with the rod then
rotated so the bead faces the samples, a vacuum drawn and the sample
shadowed. We found this gave as good a shadow as some of the prepared
Pt/C rods and was much simpler and cheaper. The sample could then be
coated , at 90°, with a layer of carbon or removed with only the Pt/C
shadowing. Drops of PAA were then applied to the areas of interest,
allowed to dry, picked off and either (Pt/C + C layers) floated as is
on water or backed on the Pt/C side (Pt/C only)with a C layer and
then floated. The C layer is strong enough to prevent breaking up of
the replica during PAA dissolution. NH4OH can be added to the water
to speed up the dissolution and methanol can be used instead of water
as the PAA solvent to speed up the drying. I don't think we have
tried methanol as the solvent for the dissolution; the surface
tension may be insufficient to float the sandwich. The PAA we are now
using is Carbopol 907, a dry powder also from Goodrich. Chuck is
correct that the MW is of concern, primarily to control the swelling
during dissolution; hopefully he can get a new supply, mine now dates
from 1988.
As indicated, in many cases it is possible to strip the
Pt/C-C layers from the polymer surface (in most cases with no
adhering polymer, but in others yielding extraction replicas).
Evidence for the lack of adhesion to the surface is that it was
possible to re-replicate surfaces at least one or two times to
observe, e.g, the effect of annealing or swelling; identical areas
could be compared. However the PAA appears to adhere less well to C
than to the PT/C, requiring the second method in some cases. Even
then, the Pt/C sometimes sticks so tight to some samples we can't pry
it off (note it has to be totally dry to prevent deformation during
removal). Sometimes, for reasons I don't understand (e.g., the vacuum
used, length of time in the evaporator, or the amount of radiation
heating during evaporation of the PT/C) just using a new sample
solves the problem. As Bob indicates one can sometimes use just the C
and get a reasonable replica. However, if all fails one can use the
PAA alone as a replicating medium, shadowing and coating it to
produce a two stage replica.
--


Phillip H. Geil; Ph. 217-333-0149 Fax 217-333-2736
Department of Materials Science and Engineering
University of Illinois
1304 W. Green St.
Urbana, IL 61801


From daemon Mon Nov 18 18:42:33 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 18 Nov 2002 20:07:46 -0500
Subject: Re: unusual messages in microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Andrew,
IXRF were the first of the companies to upgrade a working EDS detector to a
modern computer system at a reasonable cost. They have been doing this for
several years now and their product has been very popular and I've heard
nothing bad about their product. They were formed to exactly serve your kind
of system: where the detector is working but the old computer system is an
expensive pain to keep running. They are more sophisticated than that now
and offer a complete product line. There are other companies that also offer
this type of system, 4Pi and Quartz XOne are two others.
While the IXRF sales office is in Houston, I don't believe their whole
company is.
Disclaimer: I sometimes consult and do sales work with the Quartz Imaging
Corp.
----- Original Message -----
} From: "Andrew Werner" {werner-at-rosharon.oilfield.slb.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, November 18, 2002 8:42 AM


I would imagine there are a plethora of microscopists in The Congo and I
think Col. Emmanuel Koromah had a FESEM. It was bequeathed to him after the
passing of Laurent Kabila, may his soul rest in the mud.

-----Original Message-----
} From: Mike Bode [mailto:mb-at-Soft-Imaging.com]
Sent: Monday, November 18, 2002 10:41 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


I am getting several of these messages per day. These people probably
"harvest" email addresses and other addresses from list servers and such. If
it wasn't Spam and had the potential to hurt some people (although none from
this list, I hope), I would even consider them funny. Getting email from "
COL.EMMANUEL KOROMAH of the Democratic Republic of Congo I am one of close
aids to the former president of Congo Democratic LAURENT KABILA of blessed
memory, may his soul rest in peace" is certainly an honor!

For those of the listers who want to do something about these mailings,
there is a web site you can go to and read about the scam:
http://home.rica.net/alphae/419coal/. There is supposedly also a Task Force
in DC that will investigate. Their email is on the web site. However, I
don't think they will do anything unless someone lost money. I got one of
those emails from South Africa and send an email to the South African
police. They even responded that they would follow-up.

Mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "sstouden-at-thelinks.com"-at-sparc5.microscopy.com
[mailto:"sstouden-at-thelinks.com"-at-sparc5.microscopy.com]
Sent: Sunday, November 17, 2002 12:12 PM
To: Caroline Schooley
Cc: Vr. Richard Bejsak-Colloredo-Mansfeld;
Microscopy-at-sparc5.microscopy.com



but I used to get thesse same messages or similiar from the place as long
as as 1998 and before that by mail.

The State Department has a long list of problems associated with the
certain quote deals unquote or "needs" or opportunities; but it would seem
possible to trace them, since the mail server probably has a log file?

and the log file could be used trace the message back to the IP and match
the ip with the user. a few minutes to do this, unless of course it is
one of those Micro whomever servers.. then it might take a few
blue screens longer.

either way, should be possibleto discover the lister if they came threw
the list, if external to the list, then you need to ask your isp to trace
those messages out,, keep the email header details.

believe the FBI and othersecurity agencies might also

On Sat, 16 Nov 2002, Caroline Schooley wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I will be delighted to know if are these messengers proposing the
business
} } in Zimbabwe, Nigeria etc, are members of this group and microscopists?
} }
} } Keep care and be of good cheer
} }
} } Richard Bejsak-Colloredo-Mansfeld
}
} Richard -
}
} "Keep care" is quite appropriate. No, they aren't microscopists (have you
} ever heard of one with that kind of money?). It's a well-known (in the
} U.S., anyway) fraud that actually captures an occasional gullible victim.
} They've stolen MANY address lists.
}
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
} Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
}
}
}



From daemon Mon Nov 18 20:07:01 2002



From: alv-at-ukrbiz.net (by way of MicroscopyListserver)
Date: Mon, 18 Nov 2002 20:00:01 -0600
Subject: Ask-A-Microscopist: Genaplan microscope?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (alv-at-ukrbiz.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
November 18, 2002 at 16:04:34
---------------------------------------------------------------------------

Email: alv-at-ukrbiz.net
Name: Vadim Korsak

Organization: Institute for Nuclear Research

Education: Graduate College

Location: Kyiv, Ukraine

Question: I'm looking for any information about Genaplan (or
Jenaplan) microscope. Basic characteristic or manufacturers. Thanks.


---------------------------------------------------------------------------


From daemon Mon Nov 18 20:07:01 2002



From: tryin2succeednow-at-yahoo.com (by way of MicroscopyListserver)
Date: Mon, 18 Nov 2002 20:02:00 -0600
Subject: Ask-A-Microscopist: Connecting a Microscope to Computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tryin2succeednow-at-yahoo.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
November 18, 2002 at 19:04:24
---------------------------------------------------------------------------

Email: tryin2succeednow-at-yahoo.com
Name: Karen Parker

Organization: U of Phoenix

Education: Undergraduate College

Location: Eunice, La USA

Question: I'm rather new to the world of microscopes, and am
wondering if there is a microscope that can be connected to a home
computer. Intel used to make one, but it was more of a toy, and is
now discontinued. I would like to use it as I delve into anatomy. The
only ones I've run into thus far are extremely elaborate and
expensive.

Is there an inexpensive alternative for the hobbyist/future nurse
??? Thanks -Karen

---------------------------------------------------------------------------


From daemon Mon Nov 18 22:59:22 2002



From: Ephram Shizgal :      shizgal-at-delongamerica.com
Date: Mon, 18 Nov 2002 23:50:10 -0500
Subject: desktop microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When your request was pointed out to me I was sure it said Desktop
MicroSCOPES. I which case I might have been of help....

I did go to the Laguna Labs site and found many pages were
unavailable...

Ephram Shizgal


-----Original Message-----
} From: Heike Gabrisch [mailto:hgabrisc-at-uno.edu]
Sent: November 18, 2002 7:55 AM
To: Microscopy-at-sparc5.microscopy.com




Hi,

Does anybody know a vendor of Desktop Microscopist?
(Laguna Labs/ James Stanley are not responding)

Heike






From daemon Mon Nov 18 23:00:49 2002



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Tue, 19 Nov 2002 12:15:55 +0100
Subject: Hales Dialysed Iron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom:

Prolonged incubation of fixed tissues in Glycerol will cause severe swelling
of organelles . Glycerol used as a cryoprotectant for Freeze Fracture
exhibited this artifact when incubated for more than 30-60 minutes.

Good Luck,

Al Coritz,
Electron Microscopy Sciences

----- Original Message -----
} From: "Tom Phillips" {phillipst-at-missouri.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, November 18, 2002 5:20 PM


Hi

Is anyone out there using Hales Dialysed Iron method? If so on what and why
is it better than Alcian Blue etc.

If you have a good reliable 'in-house' method then I would be grateful for
a copy (attachments direct to me).

I have a lot of the standard histology texts Lillie, Carlton, Culling,
Cook, Bancroft and Stevens etc so it would really only be if you do
something different - or tips.

Thanks

Gareth

Obs/NB New postal/visiting address from July 2002!

Med vänliga hälsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 5858 1038
Fax +46 728 3747

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Huddinge Universitetssjukhus, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.



From daemon Tue Nov 19 07:40:12 2002



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Tue, 19 Nov 2002 07:27:50 -0600
Subject: RE: request for opinions on EDS system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello Andrew,

I have been running a fully eqquped IXRF EDS for about three years. In
short, I am quite satisfied.

The do not manufacture their own detectors, but can supply a third party
detector if needed.

IXRF is a small "virtual" company in that their personnel reside in various
parts of the country. To this point, this has not been a problem. In fact,
I have found them to be more flexable and responsive than many larger
companies in the EM & accessory business.

The typical system consists of a detector, a PC with a custom PCI interface
card, their "pulse processor" box (with interface if beam control is
desired), and the application software. Individual hardware components are
sufficiently inexpensive that hardware repairs (I have not needed any) are
easily done by swapping a card or "box". A new detector would likely be
warranted by the third party vendor.

Software support has been very responsive. As with any complex, limited
distribution software package, it is not perfect. Overall, however, I give
it high marks. Free revision releases are common, sometimes fixing a newly
discovered bug (so far mostly minor things), or adding features which have
been requested by their customer base.

I have no vested interest in IXRF - Just a satisfied customer.

Regards,
Woody

Woody White
McDermott Technology Inc.
McD: http://www.mtiresearch.com/
Mine: http://woody.white.home.att.net


-----------------------------------------------------------------------.


Hi,

Apologies if this is a double post - got rejected first time.

Is anyone here familiar with a company called IXRF and their EDS / digital
image capture system? They are on the web at www.ixrfsystems.com - their
customer base seems to include a number of well-known companies.

The attraction is that they are based in the same city (Houston) as our lab
- but that is a very poor reason to choose an instrument. I am running a
PGT IMIX system on an ISI DS130 SEM and whenever the PGT system crashes, it
costs quite a lot to get it fixed - and much of the cost is travel
expense. So does anyone have an opinion of IXRF or any advice or
suggestions? Thanks in advance for your guidance.

Regards,
Andrew T. Werner
Chief Metallurgist
Shaped Charge Research - Metallurgy Laboratory
Schlumberger Reservoir Completions Technology Center
14910 Airline Road, Rosharon, TX 77583-1590
Voice (281) 285-5272 Fax (281) 285-5273



From daemon Tue Nov 19 08:39:02 2002



From: Lois Anderson :      landers-at-jhmi.edu (by way of MicroscopyListserver)
Date: Tue, 19 Nov 2002 08:32:40 -0600
Subject: standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is anyone aware of any established standards for evaluation or
performance management for EM Technicians. Every institution I have
contacted seems to develop their own based on their needs.

I am however, trying to determine if there are documented standards
available anywhere. I know they exist for histology. Any suggestions
would be appreciated.


From daemon Tue Nov 19 09:15:05 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Tue, 19 Nov 2002 10:04:03 -0500
Subject: Re: SEM fresh tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Connie,
Consider trying cryo-SEM if you can find a microscope with a cryo unit.
This will allow you to quickly freeze hydrated tissue and then fracture it
to reveal a surface that has not been affected by surface contact with
liquid nitrogen, air, or cutting instruments. This may be a more efficient
and meaningful way to get tissue information in a timely manner.

ESEM can be very helpful for the right type of samples. It can be more
limiting for blocks or pieces of intact tissue such as kidney, liver, etc.
where you need to image areas not affected by surfaces mechanically cut
during
initial dissection.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907


On 11/18/02 1:52 PM, ""saram-at-duke.edu"-at-sparc5.microscopy.com"
{"saram-at-duke.edu"-at-sparc5.microscopy.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The instrumentation will depend on the answer you need. Confocal is
} certainly an option, but you might look into ESEM (the environmental SEM
} now marketed by FEICO, or FEI-Philips, or whatever they're calling
} themselves these days--they've just merged with somebody else). Confocal
} will require some sort of staining, but can see layers under the surface.
} ESEM is SEM at ambient pressure--and hence, can be done on wet tissue.
}
} Sara Miller
}
}
}
} On Mon, 18 Nov 2002 ROSSCAC-at-aol.com-at-sparc5.microscopy.com wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Good day to all on the listserver,
} } I am looking for some information about doing SEM on fresh tissue. Is
} } someone out there doing this? Would confocal work better?
} } Thanks in advance,
} } Connie Cummings
} } email:rosscac-at-aol.com
} }
} }
} }
}
} Sara E. Miller, Ph. D.
} P. O. Box 3712
} Duke University Medical Center
} Durham, NC 27710
} Ph: 919 684-3452
} FAX: 919 684-3265
}
}
}



From daemon Tue Nov 19 09:30:25 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Tue, 19 Nov 2002 09:23:03 -0600
Subject: RE: Ask-A-Microscopist: Connecting a Microscope to Computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,

I hope this link will be useful for you:
http://microscopeworld.com/video/vidflex.htm

Good luck,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

------------------------------
} ---------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (tryin2succeednow-at-yahoo.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} November 18, 2002 at 19:04:24
} --------------------------------------------------------------
} -------------
}
} Email: tryin2succeednow-at-yahoo.com
} Name: Karen Parker
}
} Organization: U of Phoenix
}
} Education: Undergraduate College
}
} Location: Eunice, La USA
}
} Question: I'm rather new to the world of microscopes, and am
} wondering if there is a microscope that can be connected to a home
} computer. Intel used to make one, but it was more of a toy, and is
} now discontinued. I would like to use it as I delve into anatomy. The
} only ones I've run into thus far are extremely elaborate and
} expensive.
}
} Is there an inexpensive alternative for the hobbyist/future nurse
} ??? Thanks -Karen
}
} --------------------------------------------------------------
} -------------
}
}


From daemon Tue Nov 19 09:56:10 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Tue, 19 Nov 2002 09:47:55 -0600
Subject: RE: SEM fresh tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I believe observation of a sectioned soft wet tissue is
impossible in a ESEM. The tissue will be covered with the layer
of "juices", and only this layer will be visible. Nevertheless,
some tissues can be observed in a ESEM (hard tissues,
epithelium and others).

Vladimir

} The instrumentation will depend on the answer you need. Confocal is
} certainly an option, but you might look into ESEM (the
} environmental SEM
} now marketed by FEICO, or FEI-Philips, or whatever they're calling
} themselves these days--they've just merged with somebody
} else). Confocal
} will require some sort of staining, but can see layers under
} the surface.
} ESEM is SEM at ambient pressure--and hence, can be done on wet tissue.
}
} Sara Miller
}
}
}
} On Mon, 18 Nov 2002 ROSSCAC-at-aol.com-at-sparc5.microscopy.com wrote:
}
} }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} ---------.
} }
} }
} } Good day to all on the listserver,
} } I am looking for some information about doing SEM on fresh
} tissue. Is
} } someone out there doing this? Would confocal work better?
} } Thanks in advance,
} } Connie Cummings
} } email:rosscac-at-aol.com
} }
} }
} }
}
} Sara E. Miller, Ph. D.
} P. O. Box 3712
} Duke University Medical Center
} Durham, NC 27710
} Ph: 919 684-3452
} FAX: 919 684-3265
}
}
}


From daemon Tue Nov 19 10:42:07 2002



From: saram-at-duke.edu
Date: Tue, 19 Nov 2002 11:33:08 -0500 (EST)
Subject: Re: standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


MSA has a certification program. Contact the business office for more
info. Their address is listed at

http://microscopy.com/


Click on Business Office/Meeting Manager.


Sara Miller



On Tue, 19 Nov 2002, Lois Anderson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is anyone aware of any established standards for evaluation or
} performance management for EM Technicians. Every institution I have
} contacted seems to develop their own based on their needs.
}
} I am however, trying to determine if there are documented standards
} available anywhere. I know they exist for histology. Any suggestions
} would be appreciated.
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Nov 19 10:42:42 2002



From: =?iso-8859-1?Q?max=5Fgra-at-libero.it?= :      max_gra-at-libero.it
Date: Tue, 19 Nov 2002 17:33:08 +0100
Subject: =?iso-8859-1?Q?fixation?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Torino 19 November 2002
(Italy)

Hi,
I´m an amateur naturalist and I like to study histological speciemen by
an optical microscope.
For fixation of tissues I´d like to use the Carnoy fixative.
Unlikely it´s quite difficult for me to buy the Chloroform. I wonder if
would be possible to use something else or to use it not at all.
Thank you.
Best Regards,
Massimo




From daemon Tue Nov 19 10:55:46 2002



From: A.G.Cullis :      A.G.Cullis-at-sheffield.ac.uk
Date: Tue, 19 Nov 2002 16:48:23 -0000
Subject: MSMXIII Conference: Final Call for Papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Institute of Physics
Royal Microscopical Society
Materials Research Society

13th International Conference on

MICROSCOPY OF SEMICONDUCTING MATERIALS

31 March – 3 April 2003, University of Cambridge, UK

************************************************
Final Call for Papers
************************************************

This international conference will focus on the state-of-the-art in the
study of the structural and electrical properties of semiconductors
by the application of transmission and scanning electron
microscopy, scanning probe microscopy, X-ray techniques and all
related assessment methods.

Special conference sessions will concentrate on recent
developments in high-resolution imaging and analytical (S)TEM,
advances in SPM and SEM applications, the important
characteristics of epitaxial layers (including III-nitrides), quantum
nanostructures (dots, wires and wells), the effects of advanced
device processing treatments (especially for silicon technology),
metal-semiconductor contacts and silicides, together with critical
device properties, FIB applications and failure analysis. Prominent
invited speakers will introduce each topic area: full details are given
at the conference web site:
http://physics.iop.org/IOP/Confs/MSM/

The Proceedings of the conference will be published and the final
call for papers has now been issued. Abstracts (deadline 2
DECEMBER 2002) should be submitted on-line at the above web
site.

Additional general conference information can be obtained from the
Secretariat at the IOP (claire.pantlin-at-iop.org). The conference
Chairmen are Prof Tony Cullis (a.g.cullis-at-sheffield.ac.uk) and Dr
Paul Midgley (pam33-at-cam.ac.uk), who may be contacted with
any scientific programme enquiries.

************************************************



Professor Tony Cullis
Dept of Electronic and Electrical Eng
University of Sheffield
Mappin Street
Sheffield
S1 3JD, UK

Tel: +44-114-222-5407
Fax: +44-114-272-6391
E-mail: a.g.cullis-at-sheffield.ac.uk

****************
Remember: The MSM XIII conference
31 March - 3 April, 2003
University of Cambridge
Secretariat: IOP, London
URL http://physics.iop.org/IOP/Confs/MSM/
****************


From daemon Tue Nov 19 11:56:01 2002



From: Dave Hall :      daveh-at-restechimage.com
Date: Tue, 19 Nov 2002 12:47:06 -0500
Subject: Ask-A-Microscopist: Connecting a Microscope to Computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen:

Depending on what you consider expensive, at least one option is
available.

Our company is admittedly a microscopy vendor and I hope this reply is
not outside the spirit of the user group. We probably have a solution
to your request. Unlike the intel product, our system would not be
considered a toy. However, to keep the cost down, we did build the
system around an "entry-level" upright microscope with a monocular
design (the Intel unit offered no eyepieces). The microscope offers a
traditional stage design with slide clips, uses built-in transmitted
light, and offers 4x, 10x, and 40x objectives. The computer connection
is through a USB interface and includes software for viewing the images
as well as capturing image files to the computer. These image files can
then be handled as any other file and e-mailed, printed, edited,
annotated, etc.

The system even includes a simple measurement software tool that allows
you to measure the distance between two points in the image and can be
calibrated to real-world units using a stage micrometer. It also
provides the angle from horizontal that is created by a line connecting
those two points.

(When not using the system in the USB-connected mode, the microscope can
be used with its 10x eyepiece (included) for normal, monocular viewing.)

Total system cost: $500. If you would like additional information,
including specifications or an image of the microscope the system is
built around, please contact me outside the group.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045 Fax (614) 921-0046
*Download our FREE image measurement utility at
http://www.imagecaliper.com.


-----Original Message-----
} From: by way of MicroscopyListserver [mailto:tryin2succeednow-at-yahoo.com]

Sent: Monday, November 18, 2002 9:02 PM
To: Microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tryin2succeednow-at-yahoo.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
November 18, 2002 at 19:04:24
------------------------------------------------------------------------
---

Email: tryin2succeednow-at-yahoo.com
Name: Karen Parker

Organization: U of Phoenix

Education: Undergraduate College

Location: Eunice, La USA

Question: I'm rather new to the world of microscopes, and am
wondering if there is a microscope that can be connected to a home
computer. Intel used to make one, but it was more of a toy, and is
now discontinued. I would like to use it as I delve into anatomy. The
only ones I've run into thus far are extremely elaborate and
expensive.

Is there an inexpensive alternative for the hobbyist/future nurse
??? Thanks -Karen

------------------------------------------------------------------------
---





From daemon Tue Nov 19 11:56:01 2002



From: Dave Hall :      daveh-at-restechimage.com
Date: Tue, 19 Nov 2002 12:47:34 -0500
Subject: Re: paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All:

I was not certain of the applicability of what I am posting until
Kestutis referenced work using cross sections for measurement.

The following link is to an article published in this month's issue of
Paint & Coatings Industry Magazine.

http://www.pcimag.com/CDA/ArticleInformation/features/BNP__Features__Ite
m/0,1846,86826,00.html


This article is about a software package designed for measuring coating
layer thickness using cross sections as suggested by Kestutis.

The upcoming release of this software is slated to be compliant with
ASTM B487 which is the ASTM Standard Test Method for Measurement of
Metal and Oxide Coating Thickness by Microscopical Examination of a
Cross Section.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045 Fax (614) 921-0046
*Download our FREE image measurement utility at
http://www.imagecaliper.com.


-----Original Message-----
} From: Kestutis Smalinskas [mailto:smalinskas-at-yahoo.com]
Sent: Monday, November 18, 2002 8:55 AM
To: Microscopy-at-sparc5.microscopy.com
Cc: maloneyb-at-fiu.edu


Barb,

I've looked at many paint samples in cross section
using metallographic methods. This was done for the
automotive industry. Primer, colorcoat, and clearcoat
were all distinguishable on the metallograph.

Stu Smalinskas
Senior Metallurgist
SKF USA
Plymouth, Michigan

------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy
Society of America

__________________________________________________
Do you Yahoo!?
Yahoo! Web Hosting - Let the expert host your site
http://webhosting.yahoo.com





From daemon Tue Nov 19 13:39:24 2002



From: saram-at-duke.edu
Date: Tue, 19 Nov 2002 14:25:41 -0500 (EST)
Subject: RE: SEM fresh tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is not impossible to observe wet tissue in an ESEM. As I said before,
the instrument chosen will depend on the information the researcher
needs--which wasn't specified in the email.

It is possible to visualize wet tissues, colonies of organisms, and other
biological specimens. You are correct in saying that the surface will
be wet; however, in certain cases, useful information is obtainable.

By the way, in fairness to a new product, I was just informed that LEO
demonstrated its first environmental SEM at the MSA meeting in August.

S Miller


On Tue, 19 Nov 2002, Dusevich, Vladimir wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I believe observation of a sectioned soft wet tissue is
} impossible in a ESEM. The tissue will be covered with the layer
} of "juices", and only this layer will be visible. Nevertheless,
} some tissues can be observed in a ESEM (hard tissues,
} epithelium and others).
}
} Vladimir
}
} } The instrumentation will depend on the answer you need. Confocal is
} } certainly an option, but you might look into ESEM (the
} } environmental SEM
} } now marketed by FEICO, or FEI-Philips, or whatever they're calling
} } themselves these days--they've just merged with somebody
} } else). Confocal
} } will require some sort of staining, but can see layers under
} } the surface.
} } ESEM is SEM at ambient pressure--and hence, can be done on wet tissue.
} }
} } Sara Miller
} }
} }
} }
} } On Mon, 18 Nov 2002 ROSSCAC-at-aol.com-at-sparc5.microscopy.com wrote:
} }
} } }
} } --------------------------------------------------------------
} } ----------
} } } The Microscopy ListServer -- Sponsor: The Microscopy
} } Society of America
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } --------------------------------------------------------------
} } ---------.
} } }
} } }
} } } Good day to all on the listserver,
} } } I am looking for some information about doing SEM on fresh
} } tissue. Is
} } } someone out there doing this? Would confocal work better?
} } } Thanks in advance,
} } } Connie Cummings
} } } email:rosscac-at-aol.com
} } }
} } }
} } }
} }
} } Sara E. Miller, Ph. D.
} } P. O. Box 3712
} } Duke University Medical Center
} } Durham, NC 27710
} } Ph: 919 684-3452
} } FAX: 919 684-3265
} }
} }
} }
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Nov 19 13:42:51 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 19 Nov 2002 12:29:17 -0700
Subject: RE: Ask-A-Microscopist: Connecting a Microscope to Computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,

Sorry, I missed the original posting. Olympus produces a digital microscope
called "Mic-D", which might do what you want to achieve. Check out their
site.

http://www.olympusamerica.com/seg_section/seg_product.asp?p=4&product=188


mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Dusevich, Vladimir [mailto:dusevichv-at-umkc.edu]
Sent: Tuesday, November 19, 2002 8:23 AM
To: Microscopy-at-sparc5.microscopy.com


Karen,

I hope this link will be useful for you:
http://microscopeworld.com/video/vidflex.htm

Good luck,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

------------------------------
} ---------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (tryin2succeednow-at-yahoo.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} November 18, 2002 at 19:04:24
} --------------------------------------------------------------
} -------------
}
} Email: tryin2succeednow-at-yahoo.com
} Name: Karen Parker
}
} Organization: U of Phoenix
}
} Education: Undergraduate College
}
} Location: Eunice, La USA
}
} Question: I'm rather new to the world of microscopes, and am
} wondering if there is a microscope that can be connected to a home
} computer. Intel used to make one, but it was more of a toy, and is
} now discontinued. I would like to use it as I delve into anatomy. The
} only ones I've run into thus far are extremely elaborate and
} expensive.
}
} Is there an inexpensive alternative for the hobbyist/future nurse
} ??? Thanks -Karen
}
} --------------------------------------------------------------
} -------------
}
}


From daemon Tue Nov 19 13:44:33 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Tue, 19 Nov 2002 13:37:37 -0600
Subject: RE: SEM fresh tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} It is not impossible to observe wet tissue in an ESEM. As I

I was talking about impossibility of observation not of
any tissue, but of sectioned tissue.

} said before,
} the instrument chosen will depend on the information the researcher
} needs--which wasn't specified in the email.
}
} It is possible to visualize wet tissues, colonies of
} organisms, and other
} biological specimens. You are correct in saying that the surface will
} be wet; however, in certain cases, useful information is obtainable.
}
} By the way, in fairness to a new product, I was just informed that LEO
} demonstrated its first environmental SEM at the MSA meeting in August.
}
} S Miller
}
}
} On Tue, 19 Nov 2002, Dusevich, Vladimir wrote:
}
} }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} ---------.
} }
} }
} } I believe observation of a sectioned soft wet tissue is
} } impossible in a ESEM. The tissue will be covered with the layer
} } of "juices", and only this layer will be visible. Nevertheless,
} } some tissues can be observed in a ESEM (hard tissues,
} } epithelium and others).
} }
} } Vladimir
} }
} } } The instrumentation will depend on the answer you need.
} Confocal is
} } } certainly an option, but you might look into ESEM (the
} } } environmental SEM
} } } now marketed by FEICO, or FEI-Philips, or whatever they're calling
} } } themselves these days--they've just merged with somebody
} } } else). Confocal
} } } will require some sort of staining, but can see layers under
} } } the surface.
} } } ESEM is SEM at ambient pressure--and hence, can be done
} on wet tissue.
} } }
} } } Sara Miller
} } }
} } }
} } }
} } } On Mon, 18 Nov 2002 ROSSCAC-at-aol.com-at-sparc5.microscopy.com wrote:
} } }
} } } }
} } } --------------------------------------------------------------
} } } ----------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy
} } } Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } } --------------------------------------------------------------
} } } ---------.
} } } }
} } } }
} } } } Good day to all on the listserver,
} } } } I am looking for some information about doing SEM on fresh
} } } tissue. Is
} } } } someone out there doing this? Would confocal work better?
} } } } Thanks in advance,
} } } } Connie Cummings
} } } } email:rosscac-at-aol.com
} } } }
} } } }
} } } }
} } }
} } } Sara E. Miller, Ph. D.
} } } P. O. Box 3712
} } } Duke University Medical Center
} } } Durham, NC 27710
} } } Ph: 919 684-3452
} } } FAX: 919 684-3265
} } }
} } }
} } }
} }
} }
} }
}
} Sara E. Miller, Ph. D.
} P. O. Box 3712
} Duke University Medical Center
} Durham, NC 27710
} Ph: 919 684-3452
} FAX: 919 684-3265
}
}


From daemon Tue Nov 19 16:15:57 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Tue, 19 Nov 2002 17:04:38 -0800
Subject: histo Diatome knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,
I use Diatome diamond knives for thin sectioning and have always been happy
with the quality. Now I need advice on the Diatome histo diamond knives. I
would like to know if people are happy with these knives (basically, are
they worth the money?) and what size do most people buy (they range from
3mm to 10mm)? We were thinking of trying the 5mm knife though mostly likely
none of our samples would be greater than 3mm.
Any advice would be appreciated. TIA, Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Tue Nov 19 17:06:46 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 19 Nov 2002 14:52:25 -0800
Subject: estimate volume?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI:

I tried this question over at the NIH Image list, but did not get much
back. Anyone here have any ideas?

} Here is the problem:
}
} We are looking at TEM whole mounts of ocean water samples. The samples
} contain all kinds of things like bacteria, diatoms, crud, etc. The pictures
} we get represent 2D shadows of the 3D objects on the grid.
}
} We would like to estimate the volume of the bacteria to do some
} calculations related to carbon budget in the system. The bacteria are rods,
} spheres, sometimes crescent shapes or spindles, and other variations from
} 'idealized' shapes.
}
} I can imagine how to separate out the individual cells and use the 'Analyze
} Particles' tool to get some data about perimeters and areas, but I get
} stuck when thinking about how to derive volumes using Image. The simplest
} thing to do is assume a symmetrical object and rotate the area to get a
} volume. Not sure how to do this, or how to handle the asymmetrical objects
} like the crescents.
}
} I am not a math brain, so this might be a simple problem. If you can help
} out it would be great.


Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Tue Nov 19 18:29:48 2002



From: saram-at-duke.edu
Date: Tue, 19 Nov 2002 19:19:45 -0500 (EST)
Subject: Re: histo Diatome knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Beth,

We love our Diatome histo knives as much as those for thins. The one I
use is 5.5 mm, and the nice thing is being able to cut really wide
semithins. We can cut tissue longitudinally oriented without having to
trim it down. I'd go for the biggest one you can afford, say at least 5
mm. And, yes, I feel they're worth the money as they save time in not
having to break glass, but the biggest advantage to us is the wide cutting
area that is smooth, sharp, and not bowed over the whole cutting area.

Sara

On Tue, 19 Nov 2002, Beth Richardson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi listers,
} I use Diatome diamond knives for thin sectioning and have always been happy
} with the quality. Now I need advice on the Diatome histo diamond knives. I
} would like to know if people are happy with these knives (basically, are
} they worth the money?) and what size do most people buy (they range from
} 3mm to 10mm)? We were thinking of trying the 5mm knife though mostly likely
} none of our samples would be greater than 3mm.
} Any advice would be appreciated. TIA, Beth
}
} **********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} **********************************************************************
}
} "And it's only the giving that makes you what you are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
} ***************************************************************************
}
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Nov 19 22:01:06 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 20 Nov 2002 16:51:34 +1300
Subject: WDS - choice of b/g points

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I'm having a bit of indecision choosing background points for the analysis of low levels
of Sr, particularly in calcite, but I like my setups to be as widely-applicable and robust
as possible.

I can't, on my JXA-840A system, easily adjust the collimator slit width, and the Sr La
peak (1.806keV) on the TAP crystal has the Si Ka (1.740) nearby, the Si Kb (1.832)
almost coincident, and a 2nd-order Ca Ka (1.8455) that manages to find its way
through the PHA also very close.

I guess the potential Si interference may not matter too much for mineralogical
applications, but one of my projects involves looking for low levels of Sr in calcites, so
the Ca interference matters very much.

The software that I use allows the setting of simple ratio-type overlap coefficients, and
does linear interpolation between two background points.

Where would those more experienced than I am place their background points?

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Nov 19 22:17:40 2002



From: j.bilde-at-risoe.dk
Date: Wed, 20 Nov 2002 10:29:06 +0100
Subject: Re: Using a Microtome to Cut Hard Materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Or should I just crank up the accelerating voltage to 25 kV, open or tune up the PHA
and do the darned Sr as the 2nd-order line on LIF?

I think I know the answer already ---- too insensitive, and I suppose it would burn up
those little old calcite grains.

Right?

rtch



} From: Ritchie Sims {rsims-at-glgnov2.auckland.ac.nz}
To: {Microscopy-at-sparc5.microscopy.com}


*If* the features are random shapes (*or* non-random shapes randomly
orientated) *and* are randomly distributed throughout your
sample volume, then you can assume that the volume fraction is the
same as the area fraction in a sample 2d section.
This is probably an application that is as easy to tackle with
stereological methods as with digital image analysis.
Chris

----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, November 19, 2002 10:52 PM


Mike Haugh asked:
} Are there any references for cutting hard materials?

Hi Mike,

Volume 31, No. 4 (July 1, 1995) of Microscopy Research and
Technique was dedicated to ultramicrotomy of hard materials.

Best regards,
Jorgen.

{:::} --- {:::} --- {:::--- {:::} --- {:::} --- {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm




From daemon Wed Nov 20 07:54:42 2002



From: Clarkson Donna R Contr USAMRD :      donna.clarkson-at-brooks.af.mil
Date: Wed, 20 Nov 2002 07:43:18 -0600
Subject: histo Diatome knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning, Beth!
I've used the Diatome histo knife for a few years now and am really
satisfied. It lasts a really long time and if you have large tissue samples,
or just want it to last even longer, they have large sizes to choose from.
At my previous job I cut a lot of large nerve specimens, yet the histo
knife still lasted an entire year! They're much better than glass and as
long as you take care of it and clean it it'll not let you down.

Of course, I have no affiliation with Diatome, I'm just a very satisfied
customer!

Best regards,

Donna R. Clarkson

Northrop Grumman Information Technology
for U S Army Medical Research Detachment
at Brooks Air Force Base
Phone (210) 536-1416
FAX (210) 536-1449
e-mail donna.clarkson-at-brooks.af.mil


-----Original Message-----
} From: Beth Richardson [mailto:beth-at-dogwood.botany.uga.edu]
Sent: Tuesday, November 19, 2002 7:05 PM
To: microscopy-at-sparc5.microscopy.com


Hi listers,
I use Diatome diamond knives for thin sectioning and have always been happy
with the quality. Now I need advice on the Diatome histo diamond knives. I
would like to know if people are happy with these knives (basically, are
they worth the money?) and what size do most people buy (they range from
3mm to 10mm)? We were thinking of trying the 5mm knife though mostly likely
none of our samples would be greater than 3mm.
Any advice would be appreciated. TIA, Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Wed Nov 20 07:58:26 2002



From: MicroRocks1-at-aol.com (by way of MicroscopyListserver)
Date: Wed, 20 Nov 2002 08:39:52 -0600
Subject: Ask-A-Microscopist:reference on confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Beth,
We have both Diatome ultrathins and histo knives and are very happy with them. We use the histos for thins when they are first
sharpened and then for thicks. If your blocks are 3 mm then you may want 5 so it gives you a little movement. We always start with
the left side and progressively go right as needed.
Good Luck,

Judy M.

Judy A. Murphy, PhD
San Joaquin Delta College
Microscopy Technology Center
5151 Pacific Ave.
Stockton, CA 95207

jmurphy-at-deltacollege.edu
209/954-5284
FAX 209/954-5600

http://www.deltacollege.org/dept/electmicro/


----- Original Message -----
} From: Beth Richardson {beth-at-dogwood.botany.uga.edu}


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (MicroRocks1-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
November 20, 2002 at 08:37:41
---------------------------------------------------------------------------

Email: MicroRocks1-at-aol.com
Name: Michael

Organization: SCSU

Education: Undergraduate College

Location: New Haven, CT. USA

Question: I'm starting to get into confocal microscopy, and I'm
looking for a one-stop reference. Exact definitions for "numerical
aperture" , "phase", "DIC"... Thank you in advance

---------------------------------------------------------------------------


From daemon Wed Nov 20 08:45:43 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 20 Nov 2002 09:36:50 -0500
Subject: Re: histo Diatome knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Beth, I haven't cut on glass in years, except to teach students how
to do it. I have 6mm Histo knives and would nver be without one
again.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Nov 20 09:39:19 2002



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 20 Nov 2002 10:30:50 -0500
Subject: RE: histo Diatome knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been using the Diatome 6mm Histo knife for -at- 5 years now, and swear
by it. It has held up with regular care and periodic resharpening and is
well worth the money (and time it saves). I would never go back to making
glass knives again (did it for too many years!).

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

} -----Original Message-----
} From: Beth Richardson [SMTP:beth-at-dogwood.botany.uga.edu]
} Sent: Tuesday, November 19, 2002 8:05 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: histo Diatome knife
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi listers,
} I use Diatome diamond knives for thin sectioning and have always been
} happy
} with the quality. Now I need advice on the Diatome histo diamond knives. I
} would like to know if people are happy with these knives (basically, are
} they worth the money?) and what size do most people buy (they range from
} 3mm to 10mm)? We were thinking of trying the 5mm knife though mostly
} likely
} none of our samples would be greater than 3mm.
} Any advice would be appreciated. TIA, Beth
}
} **********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} **********************************************************************
}
} "And it's only the giving that makes you what you are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
} **************************************************************************
} *
}
}


From daemon Wed Nov 20 11:09:36 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 20 Nov 2002 11:00:01 -0600
Subject: Cathodeluminescence/SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I'm trying to locate a lab that is equipped for color cathodeluminscent scanning EM. Does anyone out there have this capability or know of someone who does?

Thanks in advance.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Wed Nov 20 11:14:41 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Wed, 20 Nov 2002 09:08:27 -0800
Subject: Re: Using a Microtome to Cut Hard Materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike:

Several years ago, Tom Malis (CANMET) and Don Steele (Alcan) wrote an excellent
paper on Ultramicrotomy for Materials Science. I have a copy of the paper that
I received from Tom and would be pleased to send you a copy of it if you think
it would be useful. This is actually listed on our web site as well in our
Application Support Section under Technical Reports. We also have several
hundred other papers listed there that may be of interest for other
applications.

Please let me know if you would like a copy of the paper - or any of the others
on our list - and I will be happy to send it out to you.

Best regards-

David


Mike Haugh wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Listers,
}
} I am looking for advice about using a microtome to cut hard materials such
} as brass, nickel, steel, stone, silicon, etc. I have had some successes,
} many failures and nothing satisfactory. Up to now the embedding material
} used is a modified Spurr. The microtome is part of a home made automated
} system that uses stepper motors and motion control software. The sample is
} embedded in a 2.5cm long block with an 8mm square cross section. This block
} is held in a special clamp built into the microtome system. We image the
} block face using a light microscope /camera system; the cut slice is
} irrelevant. Imaging is done using either background fluorescence from the
} Spur or reflection from the object with the Spurr as a dark background.
} The cut/image sequence is done hundreds of times to obtain a 3-D image. I
} have used diamond knives (45 degree), stainless steel and home made stellite
} and tungsten carbide knives. Only the diamond has given reasonable results.
} I generally cut .2 to .5 micrometers for these hard materials. Problems
} include knife damage, excessive washboard, chipping the sample, distorting
} the sample, etc. Up to now there has been no lubricant because that
} requires significant modification to the system. I will modify and test
} lubricants in the near term.
}
} Any advice regarding embedding materials, knives, lubricants, etc. would be
} greatly appreciated. Are there any references for cutting hard materials?
} I would be glad to share my experiences.
}
} Mike Haugh
}
} Michael Haugh - Director of Operations
} Resolution - http://www.resolve3d.com
} 530 Tamal Plaza, Corte Madera, CA 94925
} Office:415/945-7360 x13 FAX:415/927-4495 Cell:415/987-9929
} Email: mhaugh-at-resolve3d.com

--
David Henriks
Vice President

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 38 years of providing Materials Processing Solutions for
Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and
confidential. This message is intended for the individual or entity addressed.
If you are not the intended recipient, please do not read, copy or disclose this
communication. Notify the sender of the mistake by calling +1-949-492-2600 and
delete this message from your system.




From daemon Wed Nov 20 11:27:59 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 20 Nov 2002 09:22:41 -0800
Subject: Re: estimate volume?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Tuesday, November 19, 2002, at 02:52 PM, Jon Krupp wrote:

} } Here is the problem:
} }
} } We are looking at TEM whole mounts of ocean water samples. The samples
} } contain all kinds of things like bacteria, diatoms, crud, etc. The
} } pictures
} } we get represent 2D shadows of the 3D objects on the grid.
} }
} } We would like to estimate the volume of the bacteria to do some
} } calculations related to carbon budget in the system. The bacteria are
} } rods,
} } spheres, sometimes crescent shapes or spindles, and other variations
} } from
} } 'idealized' shapes.
} }
} } I can imagine how to separate out the individual cells and use the
} } 'Analyze
} } Particles' tool to get some data about perimeters and areas, but I get
} } stuck when thinking about how to derive volumes using Image. The
} } simplest
} } thing to do is assume a symmetrical object and rotate the area to get
} } a
} } volume. Not sure how to do this, or how to handle the asymmetrical
} } objects
} } like the crescents.
} }
} } I am not a math brain, so this might be a simple problem. If you can
} } help
} } out it would be great.

Dear Jon,
The volume of a sphere is 4/3 pi r^3 (=1/6 pi d^3). A rod-shaped
bacterium can be approximated by two hemispheres (= 1 sphere) capping a
cylinder, so, if the diameter is d and the total length is l, the
volume is 1/6 pi d^3 + 1/4 pi d^2(l-d). The volume of a cone is 1/3 b
h, where h is the height and b is the area of the base (=1/4 pi d^2 for
a circular base), and a crescent is approximately a cylinder capped by
two cones--the length of the cylinder part is the length of a (curved)
line going along the axis of the cylinder. Spirochetes are like
crescents, but you have to use the length of the axis in three
dimensions, not the projection of the axis, which you see in the image.
I hope this gives you enough info.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Wed Nov 20 17:47:41 2002



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Thu, 21 Nov 2002 10:37:10 +1100
Subject: Re: histo Diatome knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ditto, we have 8 mm knives - regular histo and histo cryo - for really big
plant stuff, they're great, worth every cent.

}
} Hi Beth,
}
} We love our Diatome histo knives as much as those for thins. The one I
} use is 5.5 mm, and the nice thing is being able to cut really wide
} semithins. We can cut tissue longitudinally oriented without having to
} trim it down. I'd go for the biggest one you can afford, say at least 5
} mm. And, yes, I feel they're worth the money as they save time in not
} having to break glass, but the biggest advantage to us is the wide cutting
} area that is smooth, sharp, and not bowed over the whole cutting area.
}
} Sara
}
} On Tue, 19 Nov 2002, Beth Richardson wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi listers,
} } I use Diatome diamond knives for thin sectioning and have always been happy
} } with the quality. Now I need advice on the Diatome histo diamond knives. I
} } would like to know if people are happy with these knives (basically, are
} } they worth the money?) and what size do most people buy (they range from
} } 3mm to 10mm)? We were thinking of trying the 5mm knife though mostly likely
} } none of our samples would be greater than 3mm.
} } Any advice would be appreciated. TIA, Beth
} }
} } **********************************************************************
} } Beth Richardson
} } EM Lab Coordinator
} } Plant Biology Department
} } University of Georgia
} } Athens, GA 30602-7271
} }
} } Phone - (706) 542-1790 & FAX - (706) 542-1805
} }
} } "Between the two evils,
} } I always pick the one I never tried before". Mae West (1893-1980)
} } **********************************************************************
} }
} } "And it's only the giving that makes you what you are".
} } Wond'ring Aloud, Jethro Tull (Aqualung)
} }
} } ***************************************************************************
} }
} }
} }
} }
} }
}
} Sara E. Miller, Ph. D.
} P. O. Box 3712
} Duke University Medical Center
} Durham, NC 27710
} Ph: 919 684-3452
} FAX: 919 684-3265


Dr Rosemary White rosemary.white-at-csiro.au
Microscopy Centre fax 61- 2 6246 5000
CSIRO Plant Industry ph. 61- 2 6246 5475 or
GPO Box 1600 mob. 61- 0402 835 973
Canberra, ACT 2601, Australia




From daemon Thu Nov 21 02:25:25 2002



From: maryam abacha :      maryam7775-at-go.com
Date: Thu, 21 Nov 2002 04:17:24 -0800 (PST)
Subject: please help me and my Family

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill
The probability is that you cannot determine whether a circular
profile is a section of a sphere
or a cone or a cylinder. In the cases of sphere and cone the
equatorial diameter and the base, respectively,
are the least likely dimensions to be encountered in a section. In
practise you just don't have a
handle on r or d or l or b or h. What is readily accessible is the
area fraction of the section occupied by the profiles of a class of
features.
Provided the sample is homogeneous and particles are uniformly
distributed and randomly orientated (grains in granite,
for example) the area fraction in a section or polished face is
representative of the volume fraction. This will not
necessarily hold true if there is a preferred orientation, or if the
sample is layered.
Chris

} Dear Jon,
} The volume of a sphere is 4/3 pi r^3 (=1/6 pi d^3). A rod-shaped
} bacterium can be approximated by two hemispheres (= 1 sphere)
capping a
} cylinder, so, if the diameter is d and the total length is l, the
} volume is 1/6 pi d^3 + 1/4 pi d^2(l-d). The volume of a cone is 1/3
b
} h, where h is the height and b is the area of the base (=1/4 pi d^2
for
} a circular base), and a crescent is approximately a cylinder capped
by
} two cones--the length of the cylinder part is the length of a
(curved)
} line going along the axis of the cylinder. Spirochetes are like
} crescents, but you have to use the length of the axis in three
} dimensions, not the projection of the axis, which you see in the
image.
} I hope this gives you enough info.
} Yours,
} Bill Tivol
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
} ----- Original Message -----
} From: "Bill Tivol" {tivol-at-caltech.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, November 20, 2002 5:22 PM



ATTN;
PLS REPLY TO MY PRAVATE BOX maryam775-at-mailsurf.com
I am Hajia Maryam Abacha, widow of the Late Gen. Sani Abacha former Nigerian
Military Head of State who died as a result of cardiac arrest.

The name of you company appeared in one of our directories as one of the
companies my late husband wanted to do businesswith before he died.
I therefore decided to contact you in confidence so that I can be able to
move out the sum of US$35,760,000.00 ( Thirty Five Million Seven hundred and
Sixty Thousand U. S. Dollars ) which was secretly
defaced and seal in big metal box for security reasons in your account.

I personally therefore appeal to you for your urgent assistance to move this
money into your country where I believe it will be safe since I cannot leave
the country due to the restriction of movement imposed on
me and members of my family by the Nigerian government.

You can contact me through, or my family lawyer . Upon the receipt of your
acceptance to assist me, my lawyer shall arrange with you for a face to face
meeting outside Nigeria in order to liaise with him towards the effective
completion of this transaction.

However, arrangement has been put in place to move this money out of the
country in batches in a secret vault through a diplomatic security company
to any of the European country as soon as you indicate your
interest. I also want you to be assured that all necessary arrangement for
the hitch-free of thistransaction has been concluded.

Conclusively, I have decided to offer you 25% of the total sum 5% will be
for whatever expenses that will be incurred, while 70% is to be used in
buying share in your company subsequent to our free movement by the Nigerian
government.

Please reply urgently and treat with absolute confidentiality and sincerity.
PLS REPLY TO MY PRAVATE BOX maryam775-at-mailsurf.com
Best regards,

HAJIA M.


ABACHAc/o Ba







___________________________________________________
GO.com Mail
Get Your Free, Private E-mail at http://mail.go.com




From daemon Thu Nov 21 06:50:44 2002



From: michael shaffer :      michael-at-shaffer.net
Date: Thu, 21 Nov 2002 09:10:13 -0330
Subject: RE: WDS - choice of b/g points

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ritchie writes ...

} I'm having a bit of indecision choosing background points for
} the analysis of low levels of Sr, particularly in calcite,
} ...
}
} ...
}
} I guess the potential Si interference may not matter too much
} for mineralogical applications, but one of my projects
} involves looking for low levels of Sr in calcites, so
} the Ca interference matters very much.
}
} The software that I use allows the setting of simple ratio-type
} overlap coefficients, and does linear interpolation
} between two background points.
}
} ...

Correct me if I'm wrong, but your software's 2-point implimentation of
measuring background is the problem ... it's too limiting and doesn't allow
for the special cases. Your case is not the only example of a 2-point bg
determination not being practical. That is, there should be a single
location for measuring the background and applying a multiplication factor
(ratio-constant) if needed. Alternatively, measure the Sr in a "pure"
calcite for determining the bg to always use. Neglecting Si may not be
warrented either ... it would seem Ca Ka fluorescing Si Ka from near
silicates may be a problem, so any bg would be best determined away from
silicon's influence (except where justified ... i.e., silicon-free rocks).
The typical procedure would be to:

(1) Do a careful spectrometer scan(s) over the entire region of possible bg
for Sr in a variety of samples, including silicate. If 2 bg locations are
not possible, pick a single location away from silicon's interference.

(2) For determining the ratio-constant, on "pure" calcite, measure the Sr
^and^ measure the bg at the location as determined by (1). Count each for a
singnificant length of time for accurately determining the ratio-constant.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)





From daemon Thu Nov 21 08:26:46 2002



From: Wulp, Kees van der :      wulp-at-pml.tno.nl
Date: Thu, 21 Nov 2002 15:18:41 +0100
Subject: SEM: experience with XL30+Windows2000 combined with Noran Vantag

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

Has anyone experience with the combination of FEI XL30 SEM with OS=Windows
2000 and the Noran Vantage analyser OS=Windows NT4.0
I am in the process of upgrading our XL30 PC operating sytem from Windows
3.11 to Windows 2000.
This also means new software for our XL30 FEG SEM, delivered by FEI.
My main interest concerns the proper control of the microscope (XL30 with
Windows 2000) by the Vantage software (mapping, feature sizing, analysis
manager).
May be you found bugs or pitfalls in the control that I am not aware of.
Is it save to make this move ?

You may respond directly to:

C.J.M. van der Wulp
TNO-Prins Maurits Laboratorium
Division 3, dept. MB
Lange Kleiweg 137
2288 GJ Rijswijk
The Netherlands
email: wulp-at-pml.tno.nl

Thank you very much in advance

Kees van der Wulp


From daemon Thu Nov 21 09:11:55 2002



From: Lois Anderson :      landers-at-jhmi.edu (by way of MicroscopyListserver)
Date: Thu, 21 Nov 2002 09:05:56 -0600
Subject: FISH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Anyone doing InSitu Hybridization on EM? if so do you have a protocol
you can share. Specifically a P.I. is asking about FISH (
fluorescein In-Situ Hybridization). How would the Fluorescein work
in an EM scope.

Lois Anderson
Johns Hopkins University
Dept. of Pathology
Laboratory Manager
Electron Microscopy/Immunofluorescence
(410) 955-2861/(410) 614-7110
{mailto:landers-at-jhmi.edu} landers-at-jhmi.edu




From daemon Thu Nov 21 09:17:13 2002



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 21 Nov 2002 09:09:22 -0600
Subject: Re: histo Diatome knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,

I've been thinking of purchasing one of these too, but I was wondering
what type of embedding media people are using it on. We have alot of
JB-4 material to be cut at 4-6 microns and some Spurrs material we cut
at 0.5 microns. I would think I could use this type of knife for Spurrs
but what about JB-4? It's fairly sticky and I'm worried it wouldn't
clean off the knife.

Karen Pawlowski, Ph.D.
UT Dallas

Clarkson Donna R Contr USAMRD wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Good morning, Beth!
} I've used the Diatome histo knife for a few years now and am really
} satisfied. It lasts a really long time and if you have large tissue samples,
} or just want it to last even longer, they have large sizes to choose from.
} At my previous job I cut a lot of large nerve specimens, yet the histo
} knife still lasted an entire year! They're much better than glass and as
} long as you take care of it and clean it it'll not let you down.
}
} Of course, I have no affiliation with Diatome, I'm just a very satisfied
} customer!
}
} Best regards,
}
} Donna R. Clarkson
}
} Northrop Grumman Information Technology
} for U S Army Medical Research Detachment
} at Brooks Air Force Base
} Phone (210) 536-1416
} FAX (210) 536-1449
} e-mail donna.clarkson-at-brooks.af.mil
}
} -----Original Message-----
} } From: Beth Richardson [mailto:beth-at-dogwood.botany.uga.edu]
} Sent: Tuesday, November 19, 2002 7:05 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: histo Diatome knife
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi listers,
} I use Diatome diamond knives for thin sectioning and have always been happy
} with the quality. Now I need advice on the Diatome histo diamond knives. I
} would like to know if people are happy with these knives (basically, are
} they worth the money?) and what size do most people buy (they range from
} 3mm to 10mm)? We were thinking of trying the 5mm knife though mostly likely
} none of our samples would be greater than 3mm.
} Any advice would be appreciated. TIA, Beth
}
} **********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} **********************************************************************
}
} "And it's only the giving that makes you what you are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
} ***************************************************************************



From daemon Thu Nov 21 09:35:41 2002



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Thu, 21 Nov 2002 10:25:26 -0800
Subject: histo knives rock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I want to thank everyone who replied to my question about the Diatome Histo
knife...16 replies, all happy knife owners! It sounds like a good
investment to us. Thanks again for the information - I love this list. Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************




From daemon Thu Nov 21 11:22:14 2002



From: Zhaojie Zhang :      ZZhang-at-uwyo.edu
Date: Thu, 21 Nov 2002 10:10:42 -0700
Subject: Ask-A-Microscopist:reference on confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One stop shopping for references in Science may not be a good idea,
although it might be OK for Christmas. But here is something you can
start with:

Handbook of biological confocal microscopy (2nd ed) by James B. Pawley
It at least has all the terms you are looking for.


Zhaojie Zhang, Ph.D.
Director, Microscopy Facility
University of Wyoming
Laramie, WY 82071

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:MicroRocks1-at-aol.com]
Sent: Wednesday, November 20, 2002 7:40 AM
To: Microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (MicroRocks1-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
November 20, 2002 at 08:37:41
------------------------------------------------------------------------
---

Email: MicroRocks1-at-aol.com
Name: Michael

Organization: SCSU

Education: Undergraduate College

Location: New Haven, CT. USA

Question: I'm starting to get into confocal microscopy, and I'm
looking for a one-stop reference. Exact definitions for "numerical
aperture" , "phase", "DIC"... Thank you in advance

------------------------------------------------------------------------
---



From daemon Thu Nov 21 12:06:48 2002



From: Jim Haley :      haley-at-mvia.com
Date: Thu, 21 Nov 2002 12:58:37 -0500
Subject: Re: please help me and my Family

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hajia,

I wish I could help you move the $35,760,000.00 but I don't think my
back would hold out.

Could you please tell me what denomination the bills are in? I mean if
this is in $5.00 bills, I'm pretty sure I won't be able to carry it all
(plus the big metal box you mention).

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
125 Sherwood Drive
Monaca, PA 15061
voice: (724) 728-7493
fax: (412) 291-1709
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

maryam abacha wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} ATTN;
} PLS REPLY TO MY PRAVATE BOX maryam775-at-mailsurf.com
} I am Hajia Maryam Abacha, widow of the Late Gen. Sani Abacha former Nigerian
} Military Head of State who died as a result of cardiac arrest.
}
} The name of you company appeared in one of our directories as one of the
} companies my late husband wanted to do businesswith before he died.
} I therefore decided to contact you in confidence so that I can be able to
} move out the sum of US$35,760,000.00 ( Thirty Five Million Seven hundred and
} Sixty Thousand U. S. Dollars ) which was secretly
} defaced and seal in big metal box for security reasons in your account.
}
} I personally therefore appeal to you for your urgent assistance to move this
} money into your country where I believe it will be safe since I cannot leave
} the country due to the restriction of movement imposed on
} me and members of my family by the Nigerian government.
}
} You can contact me through, or my family lawyer . Upon the receipt of your
} acceptance to assist me, my lawyer shall arrange with you for a face to face
} meeting outside Nigeria in order to liaise with him towards the effective
} completion of this transaction.
}
} However, arrangement has been put in place to move this money out of the
} country in batches in a secret vault through a diplomatic security company
} to any of the European country as soon as you indicate your
} interest. I also want you to be assured that all necessary arrangement for
} the hitch-free of thistransaction has been concluded.
}
} Conclusively, I have decided to offer you 25% of the total sum 5% will be
} for whatever expenses that will be incurred, while 70% is to be used in
} buying share in your company subsequent to our free movement by the Nigerian
} government.
}
} Please reply urgently and treat with absolute confidentiality and sincerity.
} PLS REPLY TO MY PRAVATE BOX maryam775-at-mailsurf.com
} Best regards,
}
} HAJIA M.
}
} ABACHAc/o Ba
}
} ___________________________________________________
} GO.com Mail
} Get Your Free, Private E-mail at http://mail.go.com


From daemon Thu Nov 21 12:31:57 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Thu, 21 Nov 2002 12:22:10 -0600
Subject: RE: estimate volume?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,
This specimen is quite different from polished sections you
are talking about. If you extract particles from your specimen
(dissolving matrices) and put them on a TEM grid, then you will
have similar case, but stereological rule you have mentioned will
not work now. I think the easiest way is to use calculations
suggested by Bill. Of course, it is difficult to distinguish with TEM
if circular profile is a shade of sphere or cylinder, and
utilization of SEM, even stereo SEM, is preferable.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


} Bill
} The probability is that you cannot determine whether a circular
} profile is a section of a sphere
} or a cone or a cylinder. In the cases of sphere and cone the
} equatorial diameter and the base, respectively,
} are the least likely dimensions to be encountered in a section. In
} practise you just don't have a
} handle on r or d or l or b or h. What is readily accessible is the
} area fraction of the section occupied by the profiles of a class of
} features.
} Provided the sample is homogeneous and particles are uniformly
} distributed and randomly orientated (grains in granite,
} for example) the area fraction in a section or polished face is
} representative of the volume fraction. This will not
} necessarily hold true if there is a preferred orientation, or if the
} sample is layered.
} Chris
}
} } Dear Jon,
} } The volume of a sphere is 4/3 pi r^3 (=1/6 pi d^3). A rod-shaped
} } bacterium can be approximated by two hemispheres (= 1 sphere)
} capping a
} } cylinder, so, if the diameter is d and the total length is l, the
} } volume is 1/6 pi d^3 + 1/4 pi d^2(l-d). The volume of a cone is 1/3
} b
} } h, where h is the height and b is the area of the base (=1/4 pi d^2
} for
} } a circular base), and a crescent is approximately a cylinder capped
} by
} } two cones--the length of the cylinder part is the length of a
} (curved)
} } line going along the axis of the cylinder. Spirochetes are like
} } crescents, but you have to use the length of the axis in three
} } dimensions, not the projection of the axis, which you see in the
} image.
} } I hope this gives you enough info.
} } Yours,
} } Bill Tivol
} } EM Scientist and Manager
} } Cryo-Electron Microscopy Facility
} } Broad Center, Mail Code 114-96
} } California Institute of Technology
} } Pasadena CA 91125
} } (626) 395-8833
} } tivol-at-caltech.edu
} }
} } ----- Original Message -----
} } From: "Bill Tivol" {tivol-at-caltech.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, November 20, 2002 5:22 PM
} Subject: Re: estimate volume?
}
}
} } --------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------
} ---.
} }
} }
} }
} } On Tuesday, November 19, 2002, at 02:52 PM, Jon Krupp wrote:
} }
} } } } Here is the problem:
} } } }
} } } } We are looking at TEM whole mounts of ocean water samples. The
} samples
} } } } contain all kinds of things like bacteria, diatoms, crud, etc.
} The
} } } } pictures
} } } } we get represent 2D shadows of the 3D objects on the grid.
} } } }
} } } } We would like to estimate the volume of the bacteria to do some
} } } } calculations related to carbon budget in the system. The bacteria
} are
} } } } rods,
} } } } spheres, sometimes crescent shapes or spindles, and other
} variations
} } } } from
} } } } 'idealized' shapes.
} } } }
} } } } I can imagine how to separate out the individual cells and use
} the
} } } } 'Analyze
} } } } Particles' tool to get some data about perimeters and areas, but
} I get
} } } } stuck when thinking about how to derive volumes using Image. The
} } } } simplest
} } } } thing to do is assume a symmetrical object and rotate the area to
} get
} } } } a
} } } } volume. Not sure how to do this, or how to handle the
} asymmetrical
} } } } objects
} } } } like the crescents.
} } } }
} } } } I am not a math brain, so this might be a simple problem. If you
} can
} } } } help
} } } } out it would be great.
} } }


From daemon Thu Nov 21 12:53:25 2002



From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 21 Nov 2002 13:04:27 -0600
Subject: Administrivia: Don't post replys to SPAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings listers,

I just got this warning from our computer administrators. I trust them to
determine what is real and what is hoax as they are the ones who maintain
our departmental servers.

Hi All,

If you receive the following e-mail (or some variant of it), DO NOT OPEN
THE ATTACHMENT!

} From: MAILER-DAEMON-at-(recipient domain)


Colleagues...

I know it it tempting to get a laugh, but please don't post replies to the
occasional SPAM mail which gets through the server filters.

This just creates even more junk mail and we all get enough of it already.

Nestor
Your Friendly Neighborhood SysOp


From daemon Thu Nov 21 13:51:38 2002



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Thu, 21 Nov 2002 14:42:03 -0500
Subject: histo knives rock

Contents Retrieved from Microscopy Listserver Archives
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I must add another note to these testimonials regarding histoknives. In my
lab up here in the Great White North we borrowed a couple of histos from
Diatome and DDK and ran them through our 'hard materials' sectioning
routines. To our surprise the histos provided better sectioning than
covnetional 35 or 45/55 degree knives in some cases. The most outstanding
case was in sectioning of an ultrahard amorphous Fe-Nd-B magnet particulate.
The 45 and 55 degree knives simply didn't produce any sections at all. The
35 knife produced so-so sections but the knife edge was trashed. The histo
produced reproducible sections of uniform thickness and exhibited no signs
of 'dulling'. It continues to be the knife that we first use for materials
of unknown hardness, and has also impressed students at the UM materials
science workshops that are run in Tucson by the former RMC(now part of
Boeckler). My only caveat from our tests was that histos are fairly
variable in their response to hard material sectioning. Since they are
substantially cheaper, my theory is that the edge is facetted on a
microscale, which leads to what I call 'Continous Knife Marks', ie parallel
lines in the direction of sectioning that occur over the entire section. If
you can't tolerate knife marks, forget about the histo. If they are no big
deal, you might want to consider one.

Tom

Dr. Tom Malis
Science Advisor
Mineral Technology Branch
Natural Resources Canada
555 Booth St., Ottawa, Ontario
613-995-7358
malis-at-nrcan.gc.ca


-----Original Message-----
} From: Beth Richardson
To: microscopy-at-sparc5.microscopy.com
Sent: 11/21/2002 1:25 PM


I want to thank everyone who replied to my question about the Diatome
Histo
knife...16 replies, all happy knife owners! It sounds like a good
investment to us. Thanks again for the information - I love this list.
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**********************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***




From daemon Thu Nov 21 14:29:00 2002



From: sebrina nolan :      jeffjaw-at-12move.be
Date: Thu, 21 Nov 2002 15:15:58 -1700
Subject: FW: Remote Controlled Mini Hotwheels Cars

Contents Retrieved from Microscopy Listserver Archives
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=========================================================
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+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
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From daemon Thu Nov 21 15:56:16 2002



From: John Seakins :      j.seakins-at-auckland.ac.nz
Date: Fri, 22 Nov 2002 10:46:30 GMT+1200
Subject: paint identification by Raman spectroscopy

Contents Retrieved from Microscopy Listserver Archives
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Prof Robin Clark of Imperial College, London pioneered the routine
use of Raman microscopic spectroscopy to identify pigments and
oils in modern and ancient paints.

The technique illuminates the sample with monochromatic laser light
and detects the weakly scattered Raman spectrum due to molecular
vibrations in the sample. These vibrational frequencies are unique to
each pigment and can be used for identification.

Modern Raman instruments are fitted with good quality microscopes
(Leica) which permit the imaging and identification of individual
pigment particles in mixtures. Multiple layers of pigments are best
identified in cross-section.

I have used the Renishaw model 1000 spectrometer in our Chemistry
Department for the identification of pigments in modern paints used
in super yachts and roof coatings as well as ancient natural pigments
used in 16th century paintings and to decorate 2000 year old Egyptian
mummys.

John


Dr John Seakins
Chemistry Department
The University of Auckland
Private Bag 92019
Auckland, New Zealand

Room 3041/6039
23 Symonds Street
Auckland City
New Zealand
Telephone: 64-9-3737599 ext 5852
Facsimile: 64-9-3737422

email:j.seakins-at-auckland.ac.nz


From daemon Thu Nov 21 16:39:22 2002



From: John Seakins :      j.seakins-at-auckland.ac.nz
Date: Fri, 22 Nov 2002 11:32:16 GMT+1200
Subject: Identification of paint pigments by Raman spectroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Prof Robin Clark of Imperial College, London pioneered the
routine use of Raman microscopic spectroscopy to identify
pigments and oils in modern and ancient paints.

The technique illuminates the sample with monochromatic laser
light and detects the weakly scattered Raman spectrum due to
molecular vibrations in the sample. These vibrational frequencies
are unique to each pigment and can be used for identification.

Modern Raman instruments are fitted with good quality
microscopes (Leica) which permit the imaging and identification
of individual pigment particles in mixtures. Multiple layers of
pigments are best identified in cross-section.

I have used the Renishaw model 1000 spectrometer in our
Chemistry Department for the identification of pigments in
modern paints used in super yachts and roof coatings as well as
ancient natural pigments used in 16th century paintings and to
decorate 2000 year old Egyptian mummies.

John Seakins (PhD)

Dr John Seakins
Chemistry Department
The University of Auckland
Private Bag 92019
Auckland, New Zealand

Room 3041/6039
23 Symonds Street
Auckland City
New Zealand
Telephone: 64-9-3737599 ext 5852
Facsimile: 64-9-3737422

email:j.seakins-at-auckland.ac.nz


From daemon Fri Nov 22 00:55:35 2002



From: Dirk Kirch :      kirch-at-imm.rwth-aachen.de
Date: Fri, 22 Nov 2002 09:54:46 +0100
Subject: EBSD weak patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, I received a number of these undeliverable messages notifications in
the past approx. 2 weeks, whenever message size filter was turned off.
Attachment sign may or may not show up on the preview pane, but the message
size will be over 100 kb. Obviously too much for a quarter page message,
with no visible attachment. Some messages do show real postmaster e-mail
addresses. Message source/route under properties usually has several
addresses at okstate.edu. Several attachments were identified by filter as a
klez.exe virus. Message may say "reply to (postmaster e-mail) if you
received this message in error". Don't do that, it starts the virus.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: {"saram-at-duke.edu"-at-sparc5.microscopy.com}
To: msa list {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, November 21, 2002 1:45 PM


Hello!

I need help with my Jeol 6100! We are doing EBSD here at our Institut
and I have the problem that the electron flux is not high enough to get
proper Kikuchi Patterns. But I don´t really no wether this is because of
our tungsten filament ( I tried different filaments) or due to a failure of the
detecor system or any other misalignment. I have to go to the maximum
probe current to get a weak signal which produces very weak
patterns.Has anybody an idea what could be wrong.

Dirk Kirch
+++++++++++++++++++++++++++++++++++++++++

Dirk Kirch
Institut fuer Metallkunde und Metallphysik
RWTH Aachen
D-52056 Aachen
Germany

Phone : +49-241-8026861
Fax : +49-241-8022301
Internet: http://www.imm.rwth-aachen.de
E-Mail : kirch-at-imm.rwth-aachen.de

+++++++++++++++++++++++++++++++++++++++++


From daemon Fri Nov 22 08:31:55 2002



From: Clarkson Donna R Contr USAMRD :      donna.clarkson-at-brooks.af.mil
Date: Fri, 22 Nov 2002 08:21:13 -0600
Subject: Re: histo Diatome knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Karen!
I've used the histo knife for various types of resin, including Epon,
Araldite, Spurrs, LR Gold & LR White. The Araldite can be rather soft and
sticky itself, but try soaking the knife in Triton X diluted with warm
water. You could even add some ETOH or isopropanol. As for the thickness,
Dr. Malis in Ottawa says he cuts hard materials with the histo knife, so it
doesn't seem that would be a problem.

Good luck!

Donna

Northrop Grumman Information Technology
for U S Army Medical Research Detachment
at Brooks Air Force Base
Phone (210) 536-1416
FAX (210) 536-1449
e-mail donna.clarkson-at-brooks.af.mil


-----Original Message-----
} From: Karen Pawlowski [mailto:kpawlow-at-swbell.net]
Sent: Thursday, November 21, 2002 9:09 AM
To: microscopy-at-sparc5.microscopy.com


Hello everyone,

I've been thinking of purchasing one of these too, but I was wondering
what type of embedding media people are using it on. We have alot of
JB-4 material to be cut at 4-6 microns and some Spurrs material we cut
at 0.5 microns. I would think I could use this type of knife for Spurrs
but what about JB-4? It's fairly sticky and I'm worried it wouldn't
clean off the knife.

Karen Pawlowski, Ph.D.
UT Dallas

Clarkson Donna R Contr USAMRD wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Good morning, Beth!
} I've used the Diatome histo knife for a few years now and am really
} satisfied. It lasts a really long time and if you have large tissue
samples,
} or just want it to last even longer, they have large sizes to choose from.
} At my previous job I cut a lot of large nerve specimens, yet the histo
} knife still lasted an entire year! They're much better than glass and as
} long as you take care of it and clean it it'll not let you down.
}
} Of course, I have no affiliation with Diatome, I'm just a very
satisfied
} customer!
}
} Best regards,
}
} Donna R. Clarkson
}
} Northrop Grumman Information Technology
} for U S Army Medical Research Detachment
} at Brooks Air Force Base
} Phone (210) 536-1416
} FAX (210) 536-1449
} e-mail donna.clarkson-at-brooks.af.mil
}
} -----Original Message-----
} } From: Beth Richardson [mailto:beth-at-dogwood.botany.uga.edu]
} Sent: Tuesday, November 19, 2002 7:05 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: histo Diatome knife
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi listers,
} I use Diatome diamond knives for thin sectioning and have always been
happy
} with the quality. Now I need advice on the Diatome histo diamond knives. I
} would like to know if people are happy with these knives (basically, are
} they worth the money?) and what size do most people buy (they range from
} 3mm to 10mm)? We were thinking of trying the 5mm knife though mostly
likely
} none of our samples would be greater than 3mm.
} Any advice would be appreciated. TIA, Beth
}
} **********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} **********************************************************************
}
} "And it's only the giving that makes you what you are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
}
***************************************************************************



From daemon Fri Nov 22 08:53:55 2002



From: -at-fas.harvard.edu (by way of MicroscopyListserver)
Date: Fri, 22 Nov 2002 08:48:31 -0600
Subject: Ask-A-Microscopist: small handheld microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (-at-fas.harvard.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
November 21, 2002 at 10:48:22
---------------------------------------------------------------------------

Email: -at-fas.harvard.edu
Name: Michael Kelley

Organization: Harvard University

Education: Graduate College

Location: Cambridge, MA USA

Question: I'm trying to track down information re: a small handheld
microscope that has been referred to in incomplete collection records
as a "Nichols Microscope". It's 5.5m in length, 2.75cm in diameter,
has lenses at both ends, and pulls apart to reveal what appears to be
a receptacle for holding specimens (botanical?). Has anyone run
across/heard tell of such a beastie?

Thanks!

---------------------------------------------------------------------------


From daemon Fri Nov 22 08:53:55 2002



From: mkelley-at-fas.harvard.edu (by way of MicroscopyListserver)
Date: Fri, 22 Nov 2002 08:48:57 -0600
Subject: Ask-A-Microscopist: small handheld microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mkelley-at-fas.harvard.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
November 21, 2002 at 10:49:00
---------------------------------------------------------------------------

Email: mkelley-at-fas.harvard.edu
Name: Michael Kelley

Organization: Harvard University

Education: Graduate College

Location: Cambridge, MA USA

Question: I'm trying to track down information re: a small handheld
microscope that has been referred to in incomplete collection records
as a "Nichols Microscope". It's 5.5m in length, 2.75cm in diameter,
has lenses at both ends, and pulls apart to reveal what appears to be
a receptacle for holding specimens (botanical?). Has anyone run
across/heard tell of such a beastie?

Thanks!

---------------------------------------------------------------------------


From daemon Fri Nov 22 10:22:26 2002



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Fri, 22 Nov 2002 11:06:20 -0500
Subject: RE: FISH

Contents Retrieved from Microscopy Listserver Archives
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Please share protocols--I tried doing FISH in the past and had all kinds of
trouble!

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

} -----Original Message-----
} From: Lois Anderson [SMTP:landers-at-jhmi.edu]
} Sent: Thursday, November 21, 2002 10:06 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: FISH
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Anyone doing InSitu Hybridization on EM? if so do you have a protocol
} you can share. Specifically a P.I. is asking about FISH (
} fluorescein In-Situ Hybridization). How would the Fluorescein work
} in an EM scope.
}
} Lois Anderson
} Johns Hopkins University
} Dept. of Pathology
} Laboratory Manager
} Electron Microscopy/Immunofluorescence
} (410) 955-2861/(410) 614-7110
} {mailto:landers-at-jhmi.edu} landers-at-jhmi.edu
}
}


From daemon Fri Nov 22 10:32:45 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Fri, 22 Nov 2002 11:24:41 -0500
Subject: FISH

Contents Retrieved from Microscopy Listserver Archives
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Lois:

The fluorsecein FISH is a light microscopy procedure only, you can't see a
fluorescent tag by EM. However if you want to do in situ EM you can use a
gold tag for the EM version. These gold tags are used in conjunction with
biotinylated or digoxeninated probes.

You need to review a paper published in 1997(especially page 488) by Jacques
Chevalier, et al: Biotin and Digoxigenin as Labels for Light and Electron
Microscopy in Situ Hybridization Probes: Where Do We Stand? J. of
Histochemistry & Cytochemistry 45:481-491, 1997.

Also, look at one of the first published articles on in Situ EM published by
M. Binder, et al: In Situ Hybridization at the Electron Microscopic Level:
Localizatioon of Transcripts on Ultrathin Sections of Lowicryl K4M-embedded
Tissue Using Biotinylated Probes and Protein-A Gold Complexes. J. of Cell
Biology 102:1646-1653, 1986.

The most important thing to remember about probes used in in situ procedures
for EM is the 3 dimensional aspect of a probe. Usually a probe is tagged on
one end, which means the tagged end (which is what you'll be labeling with
either biotinylated or digoxigeninated gold) can be lying sideways to the
section's surface, completely upside down to the section's surface, etc....
Only when the tag is directly pointed perpendicular to the section's
horizontal surface AND is sitting just at the exposed surface of the thin
section will you be able to bind your gold tag to it.

Good Luck!

Karen Bentley

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954




-----Original Message-----
} From: Lois Anderson [mailto:landers-at-jhmi.edu]
Sent: Thursday, November 21, 2002 10:06 AM
To: Microscopy-at-sparc5.microscopy.com



Anyone doing InSitu Hybridization on EM? if so do you have a protocol
you can share. Specifically a P.I. is asking about FISH (
fluorescein In-Situ Hybridization). How would the Fluorescein work
in an EM scope.

Lois Anderson
Johns Hopkins University
Dept. of Pathology
Laboratory Manager
Electron Microscopy/Immunofluorescence
(410) 955-2861/(410) 614-7110
{mailto:landers-at-jhmi.edu} landers-at-jhmi.edu




From daemon Fri Nov 22 10:54:55 2002



From: Andrew Werner :      werner-at-rosharon.oilfield.slb.com
Date: Fri, 22 Nov 2002 10:31:55 -0600
Subject: Thanks and summary - Re: request for opinions on EDS system

Contents Retrieved from Microscopy Listserver Archives
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Thank you to everyone who took the time to respond, on and off list, to my
request. Y'all are an amazing resource. The general consensus was that
most people like the IXRF system, and some like it very much.

One comment was "...quite adequate for what we do. However, I don't feel
that we really push the limits of their capabilities..." and several others
said much the same.

On the negative side, one person wrote: "...must admit that the IXRF does
what we need for an imaging/EDS system. But here is where I need to
elaborate. IXRF seems to be a very responsive company, and they offer free
lifetime software upgrades available via the internet. But there are times
where that offer becomes essential. Many times, I felt as though the entire
package was a beta test version. Little quirks and small issues would pop
up. Nothing results threatening, and always with a work around solution,
but irritating nevertheless. So when you notice something, you must send
them an email. They will evaluate and comment. Most of the time, in the
next software release they try to incorporate the suggestions and fix
problems. Now, after a year, it is better than before, but some issues
remain..." - that is not terribly negative, more a caveat, but well worth
knowing.

The only truly negative thing anyone wrote was: "...evaluated it a few
years ago and I feel that it's inferior to other (i.e. Noran, PGT, EDAX and
Oxford). I also know a colleague that owns one and he said that if he had
another chance to buy an EDS system, he would not purchase an IXRF." - but
he did not explain the ways in which it was inferior, or why his colleague
would not purchase one.

Anyway, I've wasted enough of your time with this - but thank you all again
for your help. I intend to pursue funding but will probably wind up
patching up the PGT system, at least for the short term.

Regards,
Andrew Werner
Schlumberger SRC



From daemon Fri Nov 22 10:54:58 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 22 Nov 2002 11:46:50 -0500
Subject: Ask-A-Microscopist:reference on confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Michael,
I have added this last, because I think the length of my response
deserves some explanation. I believe there is NO "one stop" as you put it.
In this case, one should merely understand that volume is sometimes required
to straighten one's thoughts, and expose them to comment by those who really
know what they are doing. I am a beginner in CLSM. What follows is a
lengthy expression of what I have learned to date. In some matters, I would
expect to be corrected.
When I explain the operation of our confocal microscope to students
for the first time, I stop at our SEM first and explain how it generates an
image. This is because I have found the presence of oculars a distraction.
THEN, I take them to the confocal and tell them that for all intents and
purposes, the CLSM generates an image in the same way. Except for the
differences in illumination and scanning, the CLSM forms an image just as
does the SEM.
I have a recommendation for a book about imaging. As far as I am
concerned, there is no other like it for covering necessary ground. Inoue,S
and Spring, KR, "Video Microscopy, The Fundamentals", 2nd Ed., Plenum, ISBN:
0-306-45531-5. If you can find a school where it has been adopted for a
course, I understand the price for students is lower than reasonable.
My recommendation for a book for any CLSM beginner is a small one by
Sheppard, CJR and Shotton, DM, "Confocal Laser Scanning Microscopy",
Springer, ISBN: 0-387-91514-1. Price is less than $40.
You might also find some interesting and related electronic
processing instruction in a book by Givan, AL, "Flow Cytometry, First
Principles", Wiley, ISBN: 0-471-56095-2. Price is more than $40.
---------------------------------------
As a first aside, I would like to add a brief comment on the term
"image". As used today, the word "image" pertains to a 2-D data array which
can be used to project/draw a picture. A picture/photo is what we see with
our own 'digital' visual system. It is a projection of an image data set
that we collect in digital array. Image creation, NOT viewing, by a SEM or
by a confocal microscope (point-by-point) consists of the following steps.
In the first approximation there is a single horizontal scan
of the object by a beam of electrons or laser light respectfully.
The second step is to detect the effect of the beam on the
specimen by collecting the emitted secondary electrons or fluorescent
photons respectively using appropriately filtered detector - commonly,
photomultiplier tubes.
A common third step is to conduct the signal of the detected
energy(intensity), as it is collected in real time, to a video projection
system.
The fourth step is to set a limit on the duration of the
single scan, and to repeat the same duration scan over (or through)
different parallel lines of the specimen in regular stepwise fashion along
the 'Y'-axis. Thus, a horizontal raster line becomes a 1-D raster scan
consisting of a number of parallel horizontal, analog scans of an object.
The raster scan is digital in the 'Y'-axis and analog in the 'X'. That is,
until you wish to store the raster scan in a file, or even hold it in
computer memory. One can store a raster scan on tape as a timed sequence of
analog raster lines, but for display on modern, digital video displays, the
entire raster display is digitized and stored in memory. Thus, we have
digitizing of the raster line into 512, 640, 1024, 1280, etc. by processing
each raster line through an analog to digital (A/D) converter. In compuese,
this means that each subdivision of the line becomes a byte or bytes which
characterize position and intensity (for both SEM and CLSM). Once the
raster lines are digitized, the data collected from a single raster scan of
the object consists of a 2-D data array in memory that can be sent,
pixel-by-pixel to video output or storage.
------------------------------------------
As a second aside, good confocal imaging is all about resolution in
the 'Z' axis. Thus enters one of the more erudite additions to microscopic
considerations, the "Point Spread Function". When the microscopic world was
confined to the X-Y plane (my boyhood), we were primarily concerned with the
"Airy disk" and point-to-point, lateral resolution, green filters and what
not. Fortunately for we old-timers, these concepts were explained
algebraically, thus giving the impression that the science and engineering
of optics was relatively open to all. Now that we can collect "stacks" of
optical sections with our confocal systems, we are concerned with both
lateral and 3-D resolution. Out of this nuance comes the PSF! If you are a
normal biologist, the mention of the word 'function' in a context not bodily
is cause for tremors. With this in mind, it is probably a good idea to
approach this matter unobtrusively; for, if you are too overt, a physicist
or engineer will jump out of the bushes and confuse the issue with 'chicken
scratches' s/he will attest must be understood before the 'PSF' can be
grappled with. Your alimentary system ("Gulp!") will let you know which
path to take. I will allow that thinking about the problems can help to
make them at least visually/perceptually understandable.
---------------------------------------------
As to getting good definitions for microscopic terms, I would
strongly recommend the following site (http://micro.magnet.fsu.edu/primer/).
You can also get many of the things you need in PDF form simply by searching
with a string that ends with "PDF" (i.e. {microscopy PDF} ).
---------------------------------------------
Please note(1). Before he became a guru of optical microscopy,
Prof. Pawley was considered an expert in electron microscopy. Now he must be
considered an expert in both. I would strongly recommend a sneaky look at
the following:

http://www.cs.ubc.ca/spider/ladic/course/chptrpdf.htm . Prof. Pawley would
be happy to know how widely read he is - in the underground.
------------------------------------------------
Please note(2). One cannot search for {PSF} , one must USE the full
expression (including the "F" WORD!) to get any aid from search engines.

Hope this is useful,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone: 610-738-0437
eMail: fmonson-at-wcupa.edu




-----Original Message-----
} From: MicroRocks1-at-aol.com [mailto:MicroRocks1-at-aol.com]
Sent: Wednesday, November 20, 2002 9:40 AM
To: Microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (MicroRocks1-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
November 20, 2002 at 08:37:41
---------------------------------------------------------------------------

Email: MicroRocks1-at-aol.com
Name: Michael

Organization: SCSU

Education: Undergraduate College

Location: New Haven, CT. USA

Question: I'm starting to get into confocal microscopy, and I'm
looking for a one-stop reference. Exact definitions for "numerical
aperture" , "phase", "DIC"... Thank you in advance

---------------------------------------------------------------------------


From daemon Fri Nov 22 11:17:10 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 22 Nov 2002 09:11:29 -0800
Subject: Re: beware computer virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Thursday, November 21, 2002, at 10:45 AM,
"saram-at-duke.edu"-at-sparc5.microscopy.com wrote:
}
} As a general rule-of-thumb, you should NEVER open attachments unless
} you
} KNOW they are safe AND from a RELIABLE source!
}
}
Dear List,
To add to Sara's advice, I've gotten many emails recently purporting
to be from members of this list--and others have gotten email
purportedly from me--with no or little text, but with attachments.
Even if you know the "source", you do not know where the emails really
come from.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Fri Nov 22 15:58:16 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 22 Nov 2002 13:50:16 -0800
Subject: Re: beware computer virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 09:11 AM 11/22/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

There is a simple solution to this overall problem.
The first tool is to always use Norton AV or some other
good AV tool.

Then, since only the headers are faked, the rest of the
message body is either faked or filled with crap. If
all listers sign their postings, that will tell a reader
if the message is legit. My mailer (Eudora) puts my
signature at the end of the message. So I have to reply
in reverse order (original msg first, my reply after it).
I do this in this message.

See if this strategy works.


Gary Gaugler, Ph.D.
President
Microtechnics, Inc.
7970 Twin Rocks Rd
Granite Bay, CA 95746
916.791.8191
916.791.8186 fax

Digital imaging solutions.
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Version: PGP Personal Privacy 6.5.2
iQA/AwUBPdMPkjw97t+rBVN1EQKv6QCcCvv237aPXWf8nGFOzz/3RmYcnnwAn0F2
FVHijrgFbPacDGQaO8YqB++J
=HziP
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From daemon Fri Nov 22 16:57:38 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 22 Nov 2002 14:47:35 -0800
Subject: Re: beware computer virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As a matter of fact, a few successive virus attacks on my computer were
when I got messages from my friends and did not perform all
precautions. In the current situation, when nearly 10% of E.mails I've
received daily is infected, I am VERY careful with attachments: I do open
them only if DO KNOW that this particular file with this particular
filename was sent to me from particular person. Sergey

At 09:11 AM 11/22/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Sat Nov 23 09:57:19 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Sat, 23 Nov 2002 10:39:18 -0500
Subject: Re: EBSD weak patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dirk,
I haven't seen any replies to your question. Although I haven't done
any Kikuchi Patterns (selective area electron channeling patterns?), I
believe the contrast IS very low which means that you have to really
crank up the contrast. This is why most BSE detector systems have a
suppression control of some sort along with a brightness control. The
brightness usually acts on the signal near the output of the amplifier
while the suppression injects some DC signal into the front end of the
amplifier to counter-act the large DC component in a low contrast
sample. By subtracting out the DC component, the AC can be amplified
much more without saturating the amplifier.

Basically, you need to use a lot of beam current, turn the brightness
down, contrast up, and suppression up (I'm assuming that the 6100 has
this as the 840 does). This will have to be done in fairly small
increments so that you don't lose the signal all together.

Good luck.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Dirk Kirch wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Sun Nov 24 17:46:49 2002



From: jerry smith :      jsmit51-at-tampabay.rr.com
Date: Thu, 21 Nov 2002 06:32:56 -0500
Subject: parts for s570

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


does anyone have a spare anode for an s570 lab-6,
ours has just disappeared, probablly in a black hole in
some nebula.

ben ghaffari
rangets-at-aol.com



From daemon Mon Nov 25 05:59:55 2002



From: jerry smith :      jsmit51-at-tampabay.rr.com
Date: Thu, 21 Nov 2002 06:32:56 -0500
Subject: parts for s570

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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--====_ABC1234567890DEF_====

From daemon Mon Nov 25 06:05:50 2002



From: ANTIGEN_NIHEXCHANGE12 :      ANTIGEN_NIHEXCHANGE12-at-mail.nih.gov
Date: Mon, 25 Nov 2002 06:58:59 -0500
Subject: Virus file blocked!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

PLEASE DO NOT RESPOND TO THIS MESSAGE!!!

The NIH Central Email Service Virus Scanner found the README.EXE file which
matches the virus file filter of FILE FILTER= readme.exe. The file was
Removed.
The email containing this file with the subject line of
"GSC(Atlantic)", was sent from Frank Thomas
MicroscopyFilteredEmail2-at-sparc5.microscopy.com
to MicroscopyFilteredEmail2-at-sparc5.microscopy.com
MicroscopyFilteredEmail2-at-sparc5.microscopy.com.

From daemon Mon Nov 25 06:05:54 2002



From: ANTIGEN_NIDDKCH1 :      ANTIGEN_NIDDKCH1-at-intra.niddk.nih.gov
Date: Mon, 25 Nov 2002 06:56:55 -0500
Subject: Antigen found FILE FILTER= *.exe file

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Antigen for Exchange found README.EXE matching FILE FILTER= *.exe file
filter.
The file is currently Removed. The message, "GSC(Atlantic)", was
sent from Frank Thomas and was discovered in Realtime Scan Job\Marx, Richard
(NIH/NIDDK)\Inbox
located at NIH/NIDDKINTRA/NIDDKCH1.

From daemon Mon Nov 25 06:05:54 2002



From: ANTIGEN_NIDDKCH1 :      ANTIGEN_NIDDKCH1-at-intra.niddk.nih.gov
Date: Mon, 25 Nov 2002 06:56:55 -0500
Subject: Antigen found FILE FILTER= *.exe file

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Antigen for Exchange found README.EXE matching FILE FILTER= *.exe file
filter.
The file is currently Removed. The message, "GSC(Atlantic)", was
sent from Frank Thomas and was discovered in Realtime Scan Job\Marx, Richard
(NIH/NIDDK)\Inbox
located at NIH/NIDDKINTRA/NIDDKCH1.

From daemon Mon Nov 25 06:05:54 2002



From: ANTIGEN_NIHEXCHANGE4 :      ANTIGEN_NIHEXCHANGE4-at-mail.nih.gov
Date: Mon, 25 Nov 2002 06:58:59 -0500
Subject: Virus file blocked!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

PLEASE DO NOT RESPOND TO THIS MESSAGE!!!

The NIH Central Email Service Virus Scanner found the README.EXE file which
matches the virus file filter of FILE FILTER= readme.exe. The file was
Removed.
The email containing this file with the subject line of
"GSC(Atlantic)", was sent from Frank Thomas
MicroscopyFilteredEmail2-at-sparc5.microscopy.com
to MicroscopyFilteredEmail2-at-sparc5.microscopy.com
MicroscopyFilteredEmail2-at-sparc5.microscopy.com.

From daemon Mon Nov 25 06:05:54 2002



From: ANTIGEN_NIHEXCHANGE8 :      ANTIGEN_NIHEXCHANGE8-at-mail.nih.gov
Date: Mon, 25 Nov 2002 06:58:59 -0500
Subject: Virus file blocked!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

PLEASE DO NOT RESPOND TO THIS MESSAGE!!!

The NIH Central Email Service Virus Scanner found the README.EXE file which
matches the virus file filter of FILE FILTER= readme.exe. The file was
Removed.
The email containing this file with the subject line of
"GSC(Atlantic)", was sent from Frank Thomas
MicroscopyFilteredEmail2-at-sparc5.microscopy.com
to MicroscopyFilteredEmail2-at-sparc5.microscopy.com
MicroscopyFilteredEmail2-at-sparc5.microscopy.com.

From daemon Mon Nov 25 06:27:15 2002



From: System Attendant :      UCMAIL5-SA-at-UCMAIL.UC.EDU
Date: Mon, 25 Nov 2002 07:21:07 -0500
Subject: ScanMail Message: To Sender virus found or matched file blocking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ScanMail for Microsoft Exchange has taken action on the message, please
refer to the contents of this message for further details.

Sender = MicroscopyFilteredEmail2-at-sparc5.microscopy.com
Recipient(s) = MicroscopyFilteredEmail2-at-sparc5.microscopy.com;
Subject = GSC(Atlantic)
Scanning Time = 11/25/2002 07:21:07
Engine/Pattern = 6.150-1001/391

Action on message:
The attachment README.EXE matched file blocking settings. ScanMail has taken
the Deleted action.

Warning to sender. ScanMail has detected a virus in an email you sent.

From daemon Mon Nov 25 06:27:15 2002



From: System Attendant :      UCMAIL5-SA-at-UCMAIL.UC.EDU
Date: Mon, 25 Nov 2002 07:21:07 -0500
Subject: ScanMail Message: To Recipient virus found or matched file blocki

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ScanMail for Microsoft Exchange has taken action on the message, please
refer to the contents of this message for further details.

Sender = MicroscopyFilteredEmail2-at-sparc5.microscopy.com
Recipient(s) = MicroscopyFilteredEmail2-at-sparc5.microscopy.com;
Subject = GSC(Atlantic)
Scanning Time = 11/25/2002 07:21:07
Engine/Pattern = 6.150-1001/391

Action on message:
The attachment README.EXE matched file blocking settings. ScanMail has taken
the Deleted action.

Warning to recipient. ScanMail has detected a virus.

From daemon Mon Nov 25 06:43:47 2002



From: :      Administrator
Date: Mon, 25 Nov 2002 06:36:52 -0600
Subject: ScanMail Message: To Recipient virus found and action taken.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ScanMail for Microsoft Exchange has detected virus-infected attachment(s).

Sender = Frank Thomas
Recipient(s) = MicroscopyFilteredEmail2-at-sparc5.microscopy.com
Subject = GSC(Atlantic)
Scanning Time = 11/25/2002 06:36:52
Engine/Pattern = 6.150-1001/391

Action on virus found:
The attachment README.EXE contains WORM_BRAID.A virus. ScanMail has Moved it. The attachment was moved to C:\Program Files\Trend\Smex\Virus\README3de21964139.EXE_.

Warning to recipient. ScanMail has detected a virus.

If the disinfected virus is KLEZ or BUGBEAR then it is very likely that the identified SENDER address is incorrect. Please use links below for an explanation of how KLEZ and BUGBEAR spoofs the SENDER address:
http://www.uwsp.edu/it/news/modApr2902.htm
http://www.trendmicro.com/vinfo/virusencyclo/default5.asp?VName=WORM_BUGBEAR.A

Also this article does a good job of explaining how KLEZ works: {http://antivirus.about.com/library/weekly/aa042502a.htm}

If in the "Action on virus found:" section states that the attachment was moved to "C:\Program Files\Trend\Smex\Virus", you do not need to worry as the virus was quarantined to the C: drive on the server, not your personal hard disk. If there is still an attachment in the original message, it is safe to open.

For up-to-date virus information: {http://www.uwsp.edu/it/exchange/VirusInformation-TrendMicro.htm}

From daemon Mon Nov 25 06:48:30 2002



From: Antigen_EDUNIVSMTP01
Date: 25 Nov 2002 08:10:53 -0500
Subject: Antigen found VIRUS= Exploit.IFrame.FileDownload (Kaspersky) virus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Incident Information:-

Originator: Frank Thomas
{MicroscopyFilteredEmail2-at-sparc5.microscopy.com}
Recipients: MicroscopyFilteredEmail2-at-sparc5.microscopy.com

This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.

------_=_NextPart_000_01C29483.ACA52C2A
Content-Type: text/plain

Action Taken:
The attachment was deleted from the message and replaced with a text file
informing the recipient of the action taken.

To:
MicroscopyFilteredEmail2-at-sparc5.microscopy.com
{MicroscopyFilteredEmail2-at-sparc5.microscopy.com}


This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.

------_=_NextPart_001_01C29483.ACA52C2A
Content-Type: text/plain

Action Taken:
The attachment was deleted from the message and replaced with a text file
informing the recipient of the action taken.

To:
MicroscopyFilteredEmail2-at-sparc5.microscopy.com
{MicroscopyFilteredEmail2-at-sparc5.microscopy.com}


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Antigen for Exchange found Unknown infected with VIRUS= Exploit.IFrame.FileDownload (Kaspersky) virus.
The file is currently Removed. The message, "GSC", was
sent from Frank Thomas and was discovered in SMTP Messages\Inbound And Outbound
located at Exchange/UMassMed/EDUNIVSMTP01.



From daemon Mon Nov 25 08:07:09 2002



From: System Attendant :      EXCHANGE01-SA-at-exchange.bnl.gov
Date: Mon, 25 Nov 2002 08:58:21 -0500
Subject: ScanMail Message: To Sender, action taken by attachment blocking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id IAA03407
for dist-Microscopy; Mon, 25 Nov 2002 08:04:22 -0600 (CST)
Received: from hubmail.ou.edu (hubmail.oulan.ou.edu [129.15.87.252])
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with ESMTP id IAA03402
for {MicroscopyFilteredEmail2-at-sparc5.microscopy.com} ; Mon, 25 Nov 2002 08:04:20 -0600 (CST)
Received: by hubmail.oulan.ou.edu with Internet Mail Service (5.5.2656.59)
id {XH73GWFB} ; Mon, 25 Nov 2002 07:59:15 -0600
Message-ID: {F90A16A3B592D611A2C500065B39E8B202570C96-at-hubmail.oulan.ou.edu}
{NAIOULANHUBMAIL-at-msmailhub.oulan.ou.edu}
To: "'Frank Thomas'" {MicroscopyFilteredEmail2-at-sparc5.microscopy.com}
s generated

This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.

------_=_NextPart_000_01C2948A.D2175F60
Content-Type: text/plain

Action Taken:
The attachment was quarantined from the message and replaced with a text
file informing the recipient of the action taken.

To:
MicroscopyFilteredEmail2-at-sparc5.microscopy.com
{MicroscopyFilteredEmail2-at-sparc5.microscopy.com}


ScanMail for Microsoft Exchange has blocked a file attachment(s).

Place = MicroscopyFilteredEmail2-at-sparc5.microscopy.com
Sender = Frank Thomas
Subject = GSC(Atlantic)
Delivery Time = November 25, 2002 (Monday) 08:58:20

Action on file attachment(s):
Delete 'README.EXE' at Kim, Jae-Yong

Message from recipient's administrator:
Warning to sender. ScanMail has blocked a file during a real-time scan of
the email traffic.

From daemon Mon Nov 25 08:07:10 2002



From: zaluzec-at-microscopy.com
Date: Mon, 25 Nov 2002 08:27:00 -0600
Subject: Administrivia: Yes I saw the virus....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.

------_=_NextPart_000_01C2948A.D2368020
Content-Type: text/plain

Action Taken:
The attachment was quarantined from the message and replaced with a text
file informing the recipient of the action taken.

To:
MicroscopyFilteredEmail2-at-sparc5.microscopy.com
{MicroscopyFilteredEmail2-at-sparc5.microscopy.com}



Colleagues...

Yes I just saw the Email with the attached virus..
I believe I have plugged the "hole" in the Email filter
which allowed this one to get through.

Nestor
Your Friendly Neighborhood SysOp

PS. I've also blocked the Virus warning messages being sent by NIH.


From daemon Mon Nov 25 10:52:11 2002



From: Zhiyu Wang :      zhiyuw-at-attbi.com
Date: Mon, 25 Nov 2002 20:36:03 -0000
Subject: Fw: EBSD weak patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Hi, Dirk:

You need to try a Ge standard or a piece of silicon wafer (Si) to verify
your system status (setting) to see if you can get a clear Kikuchi pattern.
I run
my HITACHI S-3500 with Opal system (Oxford), and I can get pretty clear
patterns from these two materials. The SEM condition is: W-filament,
highest
emission, largest OL aperture and spot size. 20KV, high vacuum. However,
it
does not work on most of my samples from failure analysis due to
contamination from organic on the surface. To me, this technology is more
likely a demonstration, not the really useful tool in the real world. I
can
not find an idea sample (or useless in our application) witch has a tilted
70 deg. flat surface with contamination free in atomic level and truly
crystallized from my samples stream.

Thanks,

Z. Wang
Maxtor corp.
USA

} ----- Original Message -----
} From: "Dirk Kirch" {kirch-at-imm.rwth-aachen.de}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, November 22, 2002 8:54 AM
} Subject: EBSD weak patterns
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello!
}
} I need help with my Jeol 6100! We are doing EBSD here at our Institut
} and I have the problem that the electron flux is not high enough to get
} proper Kikuchi Patterns. But I don´t really no wether this is because of
} our tungsten filament ( I tried different filaments) or due to a failure
of
} the
} detecor system or any other misalignment. I have to go to the maximum
} probe current to get a weak signal which produces very weak
} patterns.Has anybody an idea what could be wrong.
}
} Dirk Kirch
} +++++++++++++++++++++++++++++++++++++++++
}
} Dirk Kirch
} Institut fuer Metallkunde und Metallphysik
} RWTH Aachen
} D-52056 Aachen
} Germany
}
} Phone : +49-241-8026861
} Fax : +49-241-8022301
} Internet: http://www.imm.rwth-aachen.de
} E-Mail : kirch-at-imm.rwth-aachen.de
}
} +++++++++++++++++++++++++++++++++++++++++
}
}



From daemon Mon Nov 25 10:52:12 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 25 Nov 2002 11:44:20 -0500
Subject: Ask-A-Microscopist: small handheld microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Mike,
Sorry to be late, but the thought just came through. If the
specimen goes between the lenses, then I have a suggestion born of a
suspicion. Hold the scope up to the light and see what happens when you
rotate the lenses, if that is possible, with respect to one another. If by
chance the brightness of the illumination changes, then it is likely that
you have a 'beastie' which has polarizing lenses on each end that can be
crossed (to extinguish the light)so that the polarizing capabilities of the
specimen can be determined. The designation would not be a microscope from
Nichols but rather a crossed nichols microscope (nichols being pronounced
"nykols" and referring to the polarizers).
If all that is false, then I have started Monday as I usually leave
Friday. Really confused and in need of rest.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone: 610-738-0437
eMail: fmonson-at-wcupa.edu


-----Original Message-----
} From: -at-fas.harvard.edu [mailto:-at-fas.harvard.edu]
Sent: Friday, November 22, 2002 9:49 AM
To: Microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (-at-fas.harvard.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
November 21, 2002 at 10:48:22
---------------------------------------------------------------------------

Email: -at-fas.harvard.edu
Name: Michael Kelley

Organization: Harvard University

Education: Graduate College

Location: Cambridge, MA USA

Question: I'm trying to track down information re: a small handheld
microscope that has been referred to in incomplete collection records
as a "Nichols Microscope". It's 5.5m in length, 2.75cm in diameter,
has lenses at both ends, and pulls apart to reveal what appears to be
a receptacle for holding specimens (botanical?). Has anyone run
across/heard tell of such a beastie?

Thanks!

---------------------------------------------------------------------------


From daemon Mon Nov 25 12:15:29 2002



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Mon, 25 Nov 2002 13:13:54 -0500
Subject: High purity C for coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

A number of years ago I purchased ultrapure C rods from a company called
Ultracarbon (now Carbone of America, I think). I am now told that they no
longer supply this product. Does anyone know where I can purchase a
similar product? I need this for carbon coating formvar support films to
be used for X-ray microanalysis of very low Ca concentrations in sections.
The "spectroscopically pure" C rods that I have tried from other sources
either have significant Ca contamination or appear to be graphite rather
than carbon. In the latter case we have to use such high heating that it
melts the formvar films we are evaporating onto.

Any suggestions would be appreciated.

Marie

Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
University of Connecticut Unit 2242
Storrs, CT 06269-2242
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Mon Nov 25 16:47:04 2002



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Tue, 26 Nov 2002 11:36:53 +1300
Subject: Mitochondria replies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

Replies to Marc Pypaert muscle / mitochondria question and my follow
up question re assessing mitochondria fixation. Thanks for the
discussion.

Some interesting reading also in Hayat, Fixation for Electron
Microscopy (Pages 277 to 282. Pg 265 to 269 and 311), and in the
Microscopy Research and Techniques Journal, Volume 59, No 2. October
15, 2002 (Page 131 through to 146).

Regards from the deep south

Allan


MY QUESTION

} Kia Ora
}
} Marcs question about mitochondria is a little timely for us.
}
} In our Unit recently we have been experiencing an increase in the
} number of investigators who are wanting to quantify mitochondria
} morphology, usually after some experimental change.
}
} eg 1, changes in muscle mitochondria in resistance trained athelete's
} (weight lifters) compared to endurance trained athelete's (runners)
} (Human)
}
} eg 2, changes in brain stem mitochondria after a particular
} anaesthetic and neuro-surgical procedure is used (Rat)
}
} eg 3, mitochondria changes in various tissues of hibernating hedgehog
} compared to non hibernating hedgehog
}
} eg 4, mitochondria changes in fish as they move from sea water
} 'lifestyle' to a fresh water 'lifestyle'.
}
} eg 5. mitchondria changes in cultured cells after biochemically
} induced. apoptosis.
}
} These projects are all looking at morphological changes of the
} mitochondria rather than chnages in the number of mitochondria.
}
} I have some niggly concerns about these projects but I can't really
} put a finger on it. Mitochondria are such sensitive souls that I am
} not entirely convinced that some of the changes being noted are not
} tissue dissection related or fixation related (ie some are perfused,
} some are immersion fixed etc) rather than actually experiment related.
}
} My niggles are not because of something really obvious like an
} obvious osmotic problem, or delays in fixative penetration, or using
} poor quality aldehyde. It is just a gut feeling. Perhaps it is
} simply different 'appearances' of mitochondria in the diferent
} tissues.
}
} So I don't have a specific question, more I would interested in
} peoples comments on assessing the quality of mitochondria fixation
} from a morphological point of view rather than a technique point of
} view.
}
} I haven't been able to find any good reference material, apart from
} that which covers technique problems, that has been able to dispel my
} niggle.
}
} Anyone got any thoughts on precautions to take in preparation when
} specifically focusing on mitochondria morphology ?

------------------------------------------------------------------------------

REPLIES


At 7:03 PM -0700 22/10/02, Sergey Ryazantsev wrote:

} Mitochondria is very sensitive to so many factors, which we
} practically could not count. Oxidative stress is one of the major
} problem here. "Well fixed" mitochondria is only "reflection" of the
} real structure, not real things itself (as any other chemically
} fixed structure). I could imagine the experiment, when we will
} take/process samples at the very identical conditions. In this case
} we will probably see very small mitochondria structure variation
} from sample to sample. In practice, there are so many factors we
} could not count/reproduce. Therefore we expect to have a diversity
} of the structure presentation from the identical organelle,
} mitochondria in our case. So, we have a "distribution" of the
} structure. If we are studying some "effect on mitochondria" then we
} have to compare two "distributions" from control and experiment. If
} the "distributions" are not cross overed, we could talk about some
} changes. This is simplification of the problem of coarse. In my
} point of view, it's relatively simply to compare such
} "distributions" on the level of morphological changes and much, much
} more difficult on quantitative level (if even possible). In
} general, the data analysis is not only scientific issue, it's also
} ethical issue. I did see people who was extremely smart
} manipulating the EM data in favor of their theories...
}
} As for direct question in original posting, just general
} recommendations: take smallest possible piece of tissue, immediately
} immerse them into the fixer and cut on smaller pieces in the fixer
} to 1x1x1 mm with new scalpel, then cut each piece to 0.5x0.5x0.5 mm,
} then move tissue in the fresh fixer and fix 1-2 hours on ice. I
} usually do everything on ice, but you may try to do first step
} (cutting tissue, 1st change of the fixer) at the room temp to reduce
} temperature shock (use FRESH fixer for the change!). Personally, I
} prefer 1x PBS instead cacodylate and 1.5% GA is enough for such
} small pieces. Then I would wash tissue with 1x PBS for 30 min and
} fix in 1% OSO4 (in PBS or just in water) for 1 h ON ICE. 30 min
} water wash thereafter. All solutions should be fresh and made from
} "EM grade" chemicals and 18 MOhm "cell culture quality" deionized
} water. I am using GA solutions no longer than one hour after
} preparation if stored on ice. OSO4 needs to be prepared just before
} use and stored on ice. Time between collecting the tissue/animal
} death and fixation should be 20-30 seconds or SHORTER if we are
} talking about "good" mitochondria fixation. The way you kill animal
} have dramatic effect on mitochondria! Good luck. Sergey.

------------------------------------------------------------------------------

At 6:45 PM -0500 24/10/02, "sstouden-at-thelinks.com"-at-sparc5.microscopy.com wrote:

} pardon me, but i would like to ask very simple question, which I am sure
} everyone else on this list but me is able to answer,
}
} what is the morphology of a standard mitochrondium:
}
} how many standard mitochrondia structures are there?
} do structures appear and disappear within the same mitochrondrium
} Does cell type of origin change the standard of the structure?
} Is there a mitochrondria database somewhere?
} what about pore type intra mitochondrial and intercompartmental?
} pore distribution function and density seems to be the big determinate of
} mitochrondiral differentiation and function.
} what are they.
} the big structure we all know, it the little ones, that I am talking
} about, some that are sometimes there and sometimes not.
}
}
}
} how are the different structures separately classified in each such
} mitochrondria?
} is there a measurement technique to determine exact location and volume of
} these intra mitochrondrial structures?
}
} is there a noticeable by experimental method difference between
} mitochrondria of one source or the other other.
}

------------------------------------------------------------------------------

At 2:39 PM +1100 28/10/02, Rosemary White wrote:

} Re. morphology of mitochondria, as noted by others below, it's quite
} variable at TEM level, and fixation will affect it substantially. Number,
} shape, size and ultrastructure of mitochondria in a particular cell type
} will vary depending on stage in cell cycle, cell physiology - whether
} quiescent or not, activated by stimulus or not, etc, etc. Also varies with
} cell type. For good ultrastructural preservation, I would use rapid
} freezing and freeze substitution in preference to chemical fixation.
} What do you mean by "pores" in mitochondria?

------------------------------------------------------------------------------

At 4:40 PM -0600 28/10/02, "sstouden-at-thelinks.com"-at-sparc5.microscopy.com wrote:

} pores = the intra compartimental openings that filter or respond to
} ligands . Most of the pores I am talking about are
} genetically constructed in response to metabolic change.
} Let me be clear, at this size level I am not sure of anything..



------------------------------------------------------------------------------

At 7:57 AM -0600 31/10/02, "sstouden-at-thelinks.com"-at-sparc5.microscopy.com wrote:

} Rs White , i notice cell cycle stage, and cell physiology. But what I do
} not notice is in which CC stage and which physiology states determine the
} morphological variants.
}
} let me start first with the question: thoughout all known variations of
} cell cycle stages and physiolgical variation: what would be the range of
} changes? that is what changes have been noted and which of the variables
} is responsible for that change.
}
} 1. DNA changes?
} 2. membrane changes?
} 3. metabolism changes?
} 4. membrane changes (both inter mitochrondrial and outer m. cytoplasmic
} chges)
} 5. organelle changes?
} 6. one for example, is receptor mediated glut 4 metabolism in
} mitochrondria: which interest me considerable.
} thanks.

------------------------------------------------------------------------------

At 10:23 AM +1000 23/10/02, Ellis, Sarah wrote:

} You are quite right about mitochondria being sensitive little
} beasts. I suggest you run a control sample through with each
} experiment. For instance, when comparing the changes in muscle
} mitochondria in resistance trained atheletes compared to endurance
} trained atheletes, you should also take through a sample of 'normal'
} muscle (non-athelete). All samples must be fixed at the same time,
} in the same manner and all should be handled identically. If you
} don't do this then it will be impossible to say for certain that any
} changes that you see are not due to some other factor.
}
} When analysing the samples in the TEM, examine the mitochondria from
} at least 30 cells and have some selection criteria such as "all
} cells examined must be healthy looking, and have a nucleus".

------------------------------------------------------------------------------
At 9:14 AM -0400 23/10/02, "ROSSCAC-at-aol.com"-at-sparc5.microscopy.com wrote:

} Good day listserver,
} Here's my opinion:
} I agree that mitochondria are very sensitive to what occurs during oxidative
} stress, death, fixation, processing, etc --- so, the only thing left is too
} COMPARE controls versus treated - IF the tissues are handled the same in all
} respects then someone with a "trained eye" can see thru the artifacts and
} process whether there really is a difference. I for one look at each group
} separately then compare the groups looking at the quality of the mitochondria
} and then the quantity. Size of mitochondria can often be so variable between
} animals (animal variation and tissue variation) that it is best to look at
} the total surface area in the cell that contains mitochondria - by doing
} this, you should be able to compare groups as well.

------------------------------------------------------------------------------
------------------------------------------------------------------------------
------------------------------------------------------------------------------
MARC'S QUESTION

At 4:48 PM -0600 21/10/02, Marc Pypaert wrote:
} I would appreciate recieveing any fixation protocol you know
} works well for mouse muscle (extensor digitorum longus (EDL)
} and epitrochlearus (EPI)). We use routinely immersion into
} 2.5% glutaraldehyde in 0.1M cacodylate pH 7.4, but have had
} often problems with mitochondrial swelling. Since this project is
} to quantify mitochondrial density, we do need perfect and
} reliable fixation of all mitos. Perfusion is not an option. Thanks
} for any advise on type of fixative, buffer and temperature.
}

------------------------------------------------------------------------------

At 12:50 PM -0400 22/10/02, Mike Delannoy wrote:
} Marc,
} Swelling would indicate a hypo-osmotic fixative solution, try adding
} some sucrose (3.5%). I prefer
} 0.1 M Hepes or Phosphate buffer to cacodylate-it's too extractive. Also I
} would add 3 mM Cacl2 or Mgcl2
} (for the phosphate) for membrane tonicity. If you can cut very small
} pieces (3mm) rapidly, that would help.
} You also may think of adding paraformaldehyde to the fix. Good Luck.
}


------------------------------------------------------------------------------

At 9:48 AM -0400 22/10/02, Leona Cohen-Gould wrote:
} Hi Marc,
} I've always had goo results in muscle (skeletal and cardiac) with
} the following fix:
} 4% pfa (methanol-free, diluted from 16% stock )
} 2.5% glutaraldehyde
} in 0.1M Na-cacodylate buffer
} My "recipe", making this up from 10ml stock vials of 16% pfa and 10%
} glut yields 40 ml of fix, to which I then add 2 ml of saturated
} picric acid (aqueous). (based on Ito & karnovsky, J Cell Bio,
} abstract 168a, 1968)
}
} Try to hold the muscles in an extended state, either by leaving them
} attached to the leg bone, or using ligature to tie them to small
} wooden sticks. This will help prevent overly contracted sarcomeres
} and give a more "textbook" appearance with M-lines, A & I bands, etc.

------------------------------------------------------------------------------

At 11:25 AM -0400 22/10/02, "saram-at-duke.edu"-at-sparc5.microscopy.com wrote:
} } There is at least one study showing that the important thing, as far a
} } shrinkage or swelling is concerned, is the osmolarity of the buffer. This
} } should be close to physiological (300 mOsm). Glutaraldehyde will add
} } about 100 mOsm for each percent, thus, a 1% glutaraldehyde solution in
} } buffer that would be 300 mOsm by itself would be ~400 mOsm. However, the
} } tonicity added by the fixaive apparently doesn't detract from the
} } morphology.
} }
} } The 0.1 M cacodylate + 4% sucrose mentioned below will be close to the
} } percent we used to use with good success. I think, however, our final
} } sucrose concentration was 3.8%. We used to make up double strength buffer
} } and double strength aqueous fix and add them together 1:1; e.g., 0.2 M
} } cacodylate containing 6.8% sucrose and 4 % aqueous glut. Our final
} } concentration after mixing them 1:1 was 0.1 M caco, 3.4% sucr, 2% glut.
} } I think 3.5% glut is slight overkill, but won't hurt anything. Some
} } folks use 5%. You could also make the buffer with an increased
} } concentration of cacodylate, but it is expensive. 1M is sufficient
} } buffering capacity, and the sucrose makes up the rest of the tonicity.
} }
} } Cacodylate will give a finer grained ultrastructure than phosphate, but
} } some folks don't want to use the arsenical. If you choose phosphate,
} } 0.135 M phosphate buffer is physiological; you can also use less phosphate
} } and make up the difference with cheap sucrose. If you use something that
} } is ionic, like NaCl, remember that it dissociates into 2 ions and you have
} } to use half as much. Also, the 3.4 % sucrose translates in molarity to
} } 0.1 M, thus if you use an ionic substitute, use molarity to calculate the
} } amount, not percent. A 1M solution of sucrose and a 0.5 M solution of
} } NaCl will give the same osmolarity.
} }
} } Additionally, a small amount (2.5 mM, i.e., 0.0025 M) of calcium is good
} } for membranes. This is not enough to alter the osmolarity very much.
} }
} } The other thing you need to do if you're interested in muscle bands (and
} } maybe mitochondrial size???) is to fasten the muscle bundle in a
} } muscle clamp before immersing in fix. Otherwise, it contracts.
} }
} } This may be more than you wanted to know, but hope some of it helps.
} }

------------------------------------------------------------------------------

At 8:07 AM -0400 22/10/02, Mary Gail Engle wrote:

} Marc,
} We use to routinely fix mouse muscle by immersion but used 3.5%
} glut. in .1M cacodylate at 4 degrees for 1.5 hours. The pieces need
} to be very small. Add 4% sucrose to the buffer washes. The pH should
} be 7.4. Osmium should be 1% in the same buffer at 4 degrees also.
} Good luck,
--
-------------------------------------------------
Allan Mitchell
Technical Manager
Otago Centre for Electron Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Tue Nov 26 01:39:11 2002



From: Witold Zielinski :      WIZIEL-at-INMAT.PW.EDU.PL
Date: Tue, 26 Nov 2002 08:20:30 +0000
Subject: Re: Fw: EBSD weak patterns

Contents Retrieved from Microscopy Listserver Archives
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*Send reply to: "Zhiyu Wang" {zhiyuw-at-attbi.com}
*From: "Zhiyu Wang" {zhiyuw-at-attbi.com}
*To: "'Microscopy-at-MSA.Microscopy.com'" {Microscopy-at-sparc5.microscopy.com}
*Subject: Fw: EBSD weak patterns
*Date sent: Mon, 25 Nov 2002 20:36:03 -0000

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Getting EBSD pattern contamination of the surface may play a role,
however the most important is deformation of the material in the
sense of dislocations prsence.

Investigating fracture surfaces one has to remember about
deformation plastic zone around the crack tip. It means that even in
well annealed materials (which is rear) there is always high density
of dislocations near the fracture surface.

In most cases the dislocations are responsible for not clear Kikuchi
patterns.

Best regards,

Witold Zielinski



* Hi, Dirk:
*
* You need to try a Ge standard or a piece of silicon wafer (Si) to verify
* your system status (setting) to see if you can get a clear Kikuchi pattern.
*I run
* my HITACHI S-3500 with Opal system (Oxford), and I can get pretty clear
* patterns from these two materials. The SEM condition is: W-filament,
*highest
* emission, largest OL aperture and spot size. 20KV, high vacuum. However,
*it
* does not work on most of my samples from failure analysis due to
* contamination from organic on the surface. To me, this technology is more
* likely a demonstration, not the really useful tool in the real world. I
*can
* not find an idea sample (or useless in our application) witch has a tilted
* 70 deg. flat surface with contamination free in atomic level and truly
* crystallized from my samples stream.
*
* Thanks,
*
* Z. Wang
* Maxtor corp.
* USA
*
*} ----- Original Message -----
*} From: "Dirk Kirch" {kirch-at-imm.rwth-aachen.de}
*} To: {Microscopy-at-sparc5.microscopy.com}
*} Sent: Friday, November 22, 2002 8:54 AM
*} Subject: EBSD weak patterns
*}
*}
*} ------------------------------------------------------------------------
*} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
*} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
*} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
*} -----------------------------------------------------------------------.
*}
*}
*} Hello!
*}
*} I need help with my Jeol 6100! We are doing EBSD here at our Institut
*} and I have the problem that the electron flux is not high enough to get
*} proper Kikuchi Patterns. But I don t really no wether this is because of
*} our tungsten filament ( I tried different filaments) or due to a failure
*of
*} the
*} detecor system or any other misalignment. I have to go to the maximum
*} probe current to get a weak signal which produces very weak
*} patterns.Has anybody an idea what could be wrong.
*}
*} Dirk Kirch
*} +++++++++++++++++++++++++++++++++++++++++
*}
*} Dirk Kirch
*} Institut fuer Metallkunde und Metallphysik
*} RWTH Aachen
*} D-52056 Aachen
*} Germany
*}
*} Phone : +49-241-8026861
*} Fax : +49-241-8022301
*} Internet: http://www.imm.rwth-aachen.de
*} E-Mail : kirch-at-imm.rwth-aachen.de
*}
*} +++++++++++++++++++++++++++++++++++++++++
*}
*}
*
*
*
:) :) :) :) :) :) :) :) :) :) :) :) :) :) :) :) ;)

Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 87 07
660 87 36
fax #: /48 22/ 848 48 75

email: wiziel-at-inmat.pw.edu.pl


From daemon Tue Nov 26 02:21:54 2002



From: vincent peters :      Rev.pastor_vincent2-at-domebox.com
Date: Tue, 26 Nov 2002 00:13:15 -0800 (PST)
Subject: Good Project / Please Call Me.

Contents Retrieved from Microscopy Listserver Archives
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PLEASE TAKE YOUR TIME AND READ THROUGH

Dear Sir,

This may come to you as a suprise,I am REV.PASTOR VINCENT PETERS the director in charge of auditing and accounting section of Union togolaise du banque Lome-Togo in west Africa with due respect and regard.

I have decided to contact you on a business transaction that will be very beneficial to both of us at the end of the transaction.

During our investigation and auditing in this bank, my department came across a very huge sum of money belonging to a deceased person(Arthur Gene Billings)who died on october 31st 1999 in a plane crash and the
fund has been dormant in his account with this Bank without any claim of the fund in our custody either from his family or relation before our discovery to this development.

Although personally, I keep this information secret within myself and partner to enable the whole plans and idea be Profitable and successful during the time of execution. The said amount is us$14M.

Meanwhile all the whole arrangement to put claim over this fund as the bonafide next of kin to the deceased,get the required approval and transfer this money to a foreign account has been put in place and directives and needed information will be relayed to you as soon as you indicate your interest and willingness to assist us and also benefit your self to this great business opportunity.

In fact I could have done this deal alone but because of my position in this country as a civil servant, we are not allowed to operate a foreign account and would eventually raise an eye brow on my side during the time of transfer because I work in this bank. This is the
actual reason why it will require a second party or fellow who will forward claims as the next of kin with affidavit of trust of oath to the Bank and also present a foreign account where he will need the money
to be re-transferred into on his request as it may be after due verification and clarification by the correspondent branch of the bank where the whole money will be remitted from to your own designation bank account.

May I at this point emphasize that this transaction is 100% risk free as I have made arrangements for a successful deal before contacting you. On smooth conclusion of this transaction, you will be entitled to 25% of the total sum as gratification, while 5% will be set aside to take care of expenses that may arise during the time of transfer and also telephone bills, while 75% will be for me and my partners.

Please, you have been adviced to keep top secret as we are still in service and intend to retire from service after we conclude this deal with you.

I will be monitoring the whole situation here in this bank until you confirm the money in your account and ask us to come down to your country for subsequent sharing of the fund according to percentages
previously indicated and further investment, either in your country or any country you advice us to invest in.

All other necessary information will be sent to you when I hear from you.

I suggest you get back to me as soon as possible stating your wish in this deal on my confidential number 228 - 912 97 63 or for security reasons on my alternative email;securityemail-at-safe-mail.net.

Yours faithfully,

REV.PASTOR VINCENT PETERS.

_____________________________________________________________
To meet someone ---} http://www.domeconnection.com
Get free email ---} http://www.domebox.com

_____________________________________________________________
Select your own custom email address for FREE! Get you-at-yourchoice.com w/No Ads, 6MB, POP & more! http://www.everyone.net/selectmail?campaign=tag


From daemon Tue Nov 26 08:55:31 2002



From: Hayes, Fred :      Fred.Hayes-at-colaik.com
Date: Tue, 26 Nov 2002 09:48:14 -0500
Subject: staining flexible PVC for TEM

Contents Retrieved from Microscopy Listserver Archives
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Any recommendations for staining fllexible PVC for TEM? References? Thanks

Fred A. Hayes
Analyst
Polymer Microscopy
Collins and Aikman
IntelliMold Systems
4651 Platt Lane
Ann Arbor, MI 48108
734-477-7029 direct
734-477-9214 fax
734-477-9212 office
www.IntelliMold.net
www.collinsaikman.com
fred.hayes-at-colaik.com



From daemon Tue Nov 26 09:37:56 2002



From: Qian-Chun Yu, MB, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Tue, 26 Nov 2002 10:30:01 -0500
Subject: RMC ultramicrotomes?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

Is the RMC series ultramicrotomes still available on the market? I am
specifically looking for a MT-7000 or MT-X? Any information is highly
appreciated!

QC Yu

Qian-Chun Yu, MB, Ph.D.
Director
Biomedical Imaging Core Laboratory
University of Pennsylvania
School of Medicine
110 Richards Building
Philadelphia, PA 19104

Phone: 215-573-7766 (Voicemail)
FAX: 215-573-2259
E-mail: qcyu-at-mail.med.upenn.edu



From daemon Tue Nov 26 10:00:59 2002



From: rcmoretz-at-att.net
Date: Tue, 26 Nov 2002 19:10:26 +0000
Subject: Re: RMC ultramicrotomes?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For years we have provided ultrapure carbon rods. A number of sources for
pure carbon rods have dried up. We should have new stock available in early
December.

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Marie E. Cantino" {cantino-at-uconnvm.uconn.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, November 25, 2002 1:13 PM


They are repped in the east by Hacker Instruments. Try them at
http://www.hackerinstruments.com

Roger Moretz, Ph.D.
Dept of Toxicology
BI Pharmaceuticals, Inc.
Ridgefield, CT
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
}
} Is the RMC series ultramicrotomes still available on the market? I am
} specifically looking for a MT-7000 or MT-X? Any information is highly
} appreciated!
}
} QC Yu
}
} Qian-Chun Yu, MB, Ph.D.
} Director
} Biomedical Imaging Core Laboratory
} University of Pennsylvania
} School of Medicine
} 110 Richards Building
} Philadelphia, PA 19104
}
} Phone: 215-573-7766 (Voicemail)
} FAX: 215-573-2259
} E-mail: qcyu-at-mail.med.upenn.edu
}
}


From daemon Tue Nov 26 14:10:37 2002



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Tue, 26 Nov 2002 15:43:48 -0500
Subject: Re: RMC ultramicrotomes?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Yu:

Yes, RMC still manufactures UMT's in Tucson Arizona.
Contact David Roberts 520-745-0001 or Dave-at-boeckeler.com


Best,

Al Coritz
Electron Microscopy Sciences / Diatome USA
----- Original Message -----
} From: "Qian-Chun Yu, MB, Ph.D." {qcyu-at-mail.med.upenn.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, November 26, 2002 10:30 AM


Marie (and others);

As we make carbon brushes, I thought I'd ask our materials guys about
this. They didn't have any specific ideas, but gave me the name of one
of their contacts. You might be able to get some help from:

Mike Phelps
708-301-5237

He supplies us with some of our rod material, and may have a line on
"pure" carbon.

Hope this helps.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Marie E. Cantino [mailto:cantino-at-uconnvm.uconn.edu]
Sent: Monday, November 25, 2002 12:14 PM
To: Microscopy-at-sparc5.microscopy.com


Dave Roberts is the Director of EM Products
dave-at-boeckeler.com
www.rmcproducts.com

At 10:30 AM 11/26/2002 -0500, Qian-Chun Yu, MB, Ph.D. wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

****************************************************************************
Frank Macaluso tel: 718-430-3547
Analytical Imaging Facility fax: 718-430-8996
Albert Einstein College of Medicine e-mail: macaluso-at-aecom.yu.edu
1300 Morris Park Avenue http://www.aecom.yu.edu/aif/
Bronx, NY 10461
****************************************************************************




From daemon Tue Nov 26 18:08:41 2002



From: BLACKFORD, Mark G :      mgb-at-ansto.gov.au
Date: Wed, 27 Nov 2002 10:56:44 +1100
Subject: spherical abberation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

a colleague wishes to know what the spherical abberation of our JEOL 2000fxII TEM. The polepiece is AHP-20L. He only needs an approximate figure.

If anyone can help I would appreciate it. Cheers,


Mark Blackford
Materials Division, ANSTO
PMB 1,
Menai, N.S.W., 2234
Australia

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the official views of ANSTO from which this message was conveyed.



From daemon Tue Nov 26 18:11:14 2002



From: Albert Coritz :      cactusgrower-at-earthlink.net
Date: Tue, 26 Nov 2002 16:03:15 -0800
Subject: Re: RMC ultramicrotomes?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Roger:

Hacker is no longer their east coast reps. It's best to contact them
directly.

Best,

Al Coritz
Electron Microscopy Sciences/ Diatome US

On Tue, 26 Nov 2002 19:10:26 +0000 "rcmoretz-at-att.net"-at-sparc5.microscopy.com
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} They are repped in the east by Hacker
} Instruments. Try them at
} http://www.hackerinstruments.com
}
} Roger Moretz, Ph.D.
} Dept of Toxicology
} BI Pharmaceuticals, Inc.
} Ridgefield, CT
} --
} Where the world is only slightly
} less weird than it actually is.
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} }
} } Dear Colleagues,
} }
} } Is the RMC series ultramicrotomes still
} available on the market? I am
} } specifically looking for a MT-7000 or MT-X?
} Any information is highly
} } appreciated!
} }
} } QC Yu
} }
} } Qian-Chun Yu, MB, Ph.D.
} } Director
} } Biomedical Imaging Core Laboratory
} } University of Pennsylvania
} } School of Medicine
} } 110 Richards Building
} } Philadelphia, PA 19104
} }
} } Phone: 215-573-7766 (Voicemail)
} } FAX: 215-573-2259
} } E-mail: qcyu-at-mail.med.upenn.edu
} }
} }
}
}



From daemon Tue Nov 26 18:57:23 2002



From: Colin.Veitch-at-csiro.au
Date: Wed, 27 Nov 2002 11:48:38 +1100
Subject: TEM/SPM - conducting embedding resins

Contents Retrieved from Microscopy Listserver Archives
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G'day All.
 
We are currently looking at fibres using both SPM and TEM which we routinely
embed in Spurr's.  For a number of reasons we would like to attempt to embed
in a conducting medium.  Has anyone doped Spurr's to form a conducting
medium and if so, would they please tell us how?
 
It is possible to purchase a conducting embedding medium, but at this stage
we just want a quick attempt and the quantity we would need to purchase
would be far too much for our initial needs.  If there is anyone in
Australia who would be willing to donate a small amount of conducting resin,
that too would be much appreciated.
 
Thank you very much. 
 
Colin Veitch
 
Instrumentation Scientist
Late Stage Innovation Group
CSIRO Textile and Fibre Technology
PO Box 21, BELMONT, Vic. 3216. Australia.
 
E-mail:  colin.veitch-at-csiro.au
Web:    http://www.tft.csiro.au
 
Tel:       +61 (0) 3 5246 4000
Fax:      +61 (0) 3 5246 4811
 
 
The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may not
copy, distribute or take any action in reliance on it. If you have received
this message in error, please telephone CSIRO Textile and Fibre Technology
on +61 3 5246 4000.
 
 
 


From daemon Tue Nov 26 19:08:56 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 26 Nov 2002 20:00:03 -0500
Subject: RMC ultramicrotomes?

Contents Retrieved from Microscopy Listserver Archives
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Evening QC,

Hacker Instruments is the local supplier for RMC, and there is a
link on their web page to RMC.

http://www.hackerinstruments.com/

Regards,

Fred Monson (not a company rep, but I liked their ultramicrotome).

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone: 610-738-0437
eMail: fmonson-at-wcupa.edu


-----Original Message-----
} From: Qian-Chun Yu, MB, Ph.D. [mailto:qcyu-at-mail.med.upenn.edu]
Sent: Tuesday, November 26, 2002 10:30 AM
To: Microscopy-at-sparc5.microscopy.com


Dear Colleagues,

Is the RMC series ultramicrotomes still available on the market? I am
specifically looking for a MT-7000 or MT-X? Any information is highly
appreciated!

QC Yu

Qian-Chun Yu, MB, Ph.D.
Director
Biomedical Imaging Core Laboratory
University of Pennsylvania
School of Medicine
110 Richards Building
Philadelphia, PA 19104

Phone: 215-573-7766 (Voicemail)
FAX: 215-573-2259
E-mail: qcyu-at-mail.med.upenn.edu



From daemon Tue Nov 26 21:12:50 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 26 Nov 2002 21:58:19 -0500
Subject: Availability of carbon rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Marie E. Cantino wrote:
==================================================
A number of years ago I purchased ultrapure C rods from a company called
Ultracarbon (now Carbone of America, I think). I am now told that they no
longer supply this product. Does anyone know where I can purchase a similar
product? I need this for carbon coating formvar support films to be used
for X-ray microanalysis of very low Ca concentrations in sections. The
"spectroscopically pure" C rods that I have tried from other sources either
have significant Ca contamination or appear to be graphite rather than
carbon. In the latter case we have to use such high heating that it melts
the formvar films we are evaporating onto.

Any suggestions would be appreciated.
===============================================================
Carbon rods are not the same as graphite rods. Some suppliers in our
industry offer graphite rods but describe them as carbon rods. You might
want to see our URL
http://www.2spi.com/catalog/spec_prep/carbon-rods.shtml
on the SPI Supplies website for more discussion on carbon vs. graphite rods.

"Real" carbon rods (and not graphite rods) have been offered by SPI Supplies
for some time. The diameters (and package sizes) listed on the website are
in stock and are available for immediate shipment. We guarantee that there
will be no calcium contamination. And we guarantee that these are not
graphite and are as described, namely carbon. We have not ever detected Ca
in these products nor are we aware of anyone else who has detected Ca either

From daemon Tue Nov 26 21:16:26 2002



From: Benjamin - Simkin :      simkin-at-egr.msu.edu
Date: Tue, 26 Nov 2002 22:08:55 -0500 (EST)
Subject: Re: EBSD weak patterns

Contents Retrieved from Microscopy Listserver Archives
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Sorry to get in so late on this, but another factor you might consider is your
sample-EBSD detector screen distance. I don't know if your system even includes
this as a operator-controled variable, but I have found on our system that the
differance between strong patterns and weak patterns has sometimes been just
a matter of reducing the screen-sample distance by 7mm or so, with all other
parameters identical. This does require a differant calibration for the shorter
detector distance (not to mention greater care in manuvering the sample), but
if you get in the habit of keeping several differant calibrations handy (our
system loads then from differant calibration files as needed), then this can be
a 30 second fix.
I agree with the other posters about dislocation density and surface
contamination degrading EBSD signal quality, but I have found that unless I'm
working in a truely grubby system (polymer mounted samples+carbon tape, with
roughing pump backstreaming, or even worse, samples handled by bare hands and
left uncleaned) I can get patterns from selected areas (rather than an OIM
scan) fairly easily. Some qualifiers to this are: extremely low Z samples
(carbon), and extremy high dislocation density.
A partial solution to high dislocation density might be to electropolish
(or even just etch) your surface to minimise polishing damage, and surface
contamination problems can often be minimised by 'fixing' your surface
contamination by scanning as large an area of your surface with an out-of-focus
, high current beam for several minutes prior to starting work. This tends to
fix the contamination in a thin, immobile layer, so it won't diffuse to your
area of interest and fix there. A qualifier to this is: DON'T hit anything like
carbon tape, etc. with your beam. That will spew a virtual blanket over your
surface, and ruin your chances of seeing EBSD patterns.

I hope this helps,

Ben Simkin
Department of Chemical Engineering and Materials Science,
Michigan State University
simkin-at-egr.msu.edu


From daemon Wed Nov 27 08:20:08 2002



From: Qian-Chun Yu, MB, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Wed, 27 Nov 2002 09:03:47 -0500
Subject: RMC Ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

My heartfelt thanks to you all for the remarkable help. It is truly a
wonderful feeling. With that information, I already contacted RMC and
resumed my long lost connection.

I know many of you, including several very close friends, love the
Ultracut; some of you even tried hard to get me into the Ultracut. But I
am still a die-hard MT user since their MT-2 and too late to change that
feeling. This, unfortunately, is a very personal choice of taste, just
like the wines or cigars.

Thanks again for giving me the timely response, and I wish all of you a
warm, safe, and very happy "National Turkey Day".

QC Yu

Qian-Chun Yu, MB, Ph.D.
Director
Biomedical Imaging Core Laboratory
University of Pennsylvania
School of Medicine
110 Richards Building
Philadelphia, PA 19104

Phone: 215-573-7766 (Voicemail)
FAX: 215-573-2259
E-mail: qcyu-at-mail.med.upenn.edu



From daemon Wed Nov 27 08:28:18 2002



From: John Shields :      jshields-at-cb.uga.edu
Date: Wed, 27 Nov 2002 09:20:55 -0500 (Eastern Standard Time)
Subject: x-ray of resins

Contents Retrieved from Microscopy Listserver Archives
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Hi out there,
I have several people that wish to look at possible metal uptake in bacteria
and would prefer to enbed them in either Spurr's or Epon, however there
might be a problem with trace metals in these resins.
Questions are:
1. Is this a real concern? and
2. Would another resin be more suitable?

I'm trying to avoid cryo here.
Thanks
john


From daemon Wed Nov 27 08:43:52 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 27 Nov 2002 09:33:49 -0500
Subject: Re: RMC ultramicrotomes?

Contents Retrieved from Microscopy Listserver Archives
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Yes, contact Boeckeler Instruments in Tucson AZ at: 520-745-0004
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Nov 27 13:28:43 2002



From: Paul.Nolan-at-alcan.com
Date: Wed, 27 Nov 2002 14:18:41 -0500
Subject: Ultra cut E

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there.
So who is/are the sales reps for Reichert in my neighborhood.
I would like to purchase a couple more chucks for the Ultracut E
Thanks

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



From daemon Wed Nov 27 15:01:03 2002



From: Margaret.HargerAllen-at-med.va.gov
Date: Wed, 27 Nov 2002 14:54:44 -0600
Subject: Platelets/Hermansky-Pudlak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for recent TEM images and articles on platelets in
Hermansky-Pudlak disease. Thank you in advance for any help, Peggy
Harger-Allen


From daemon Wed Nov 27 15:32:48 2002



From: Shu-You Li :      syli-at-northwestern.edu
Date: Wed, 27 Nov 2002 16:46:29 -0600
Subject: cutting very hard materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just some suggestions concerning your contamination and heating problems:

Contamination:
1. Use speactroscopically pure carbon or graphite which should eliminate
your Ca problem.

Heating:
1. Try moving the substrate further from the heat source.
2. Try graphite. Since it's more crystallized it should require the same
or less heat. make sure your blank is carbon.

Since carbon and graphite come in three basic levels of purity we can supply
whatever grade you need.

Thanks for your interest.

John Arnott



Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Marie E. Cantino" {cantino-at-uconnvm.uconn.edu}
To: "John Arnott" {ladres-at-worldnet.att.net}
Sent: Tuesday, November 26, 2002 11:45 AM


Hi,
I am trying to find an instrument that has medium precision but high
speed for cutting very hard materials, Sapphire, Zirconia etc. The only
product I found is diamond band saw by SBT. Is there any competition out
there? any comments and cons/pro are also appreciated.

Thank you. Happy Thanksgiving!
Shuyou.

_____________________________
Shu-You Li, Ph.D.
Electron Microscopist
Electron Probe Instrumentation Center(EPIC)
Northwestern University
2220 Campus Drive, 1141 Cook Hall
Evanston, IL 60208, USA
Ph: (847) 491-7798, Fax: (847) 491-7820
Email: syli-at-northwestern.edu; syli16-at-hotmail.com
http://pubweb.northwestern.edu/~sli974





From daemon Wed Nov 27 21:19:00 2002



From: rcmoretz-at-att.net
Date: Thu, 28 Nov 2002 03:09:13 +0000
Subject: Re: Ultra cut E

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul:
I don't know who the reps are in your area, but you should be calling Leica.
However...I have been told be Leica and some independent service people that
the Ultracut E is so old that even routine parts (bulbs, electronics, belts,
etc) are no longer being made, so be prepared to have to look beyond Leica.

Roger Moretz, Ph.D.
Dept of Toxicology
BI Pharmaceuticals
Ridgefield, CT
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi there.
} So who is/are the sales reps for Reichert in my neighborhood.
} I would like to purchase a couple more chucks for the Ultracut E
} Thanks
}
} Paul D. Nolan
} Electron Optics
}
} Alcan International Limited
} Kingston Research and Development Centre
} P.O.Box 8400, 945 Princess Street
} Kingston, Ontario K7L 5L9
}
} Tel: (613) 541-2066
} Fax: (613) 541-2134
} paul.nolan-at-alcan.com
}
}


From daemon Wed Nov 27 23:17:54 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 27 Nov 2002 21:11:36 -0800
Subject: Re: x-ray of resins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Wednesday, November 27, 2002, at 06:20 AM, John Shields wrote:

} I have several people that wish to look at possible metal uptake in
} bacteria
} and would prefer to enbed them in either Spurr's or Epon, however there
} might be a problem with trace metals in these resins.
} Questions are:
} 1. Is this a real concern? and
} 2. Would another resin be more suitable?
}
} I'm trying to avoid cryo here.
} Thanks

Dear John,
I have looked at metals in embedded tissues, but I have not examined
the same tissues before embedding. I don't think that the metals I
looked at moved around, but they were not very active chemically, so
my experience might not apply to your problem. I would caution you,
however, that EDX is not good for trace metals; the atom fraction has
to be ~0.1% (in the volume where the x-rays are produced), and, if you
are looking for Pb and the bacteria contain S (or other overlapping
lines) you won't see Pb unless you can examine the higher energy Pb
lines. In other words, the resin may be the least of your problems.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From daemon Thu Nov 28 01:38:39 2002



From: R. Cross :      r.cross-at-ru.ac.za
Date: Thu, 28 Nov 2002 09:29:05 +0200
Subject: Used SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear SEM users (or ex-SEM users)

We are looking for a used SEM up to about 8 years old. No special
requirements other than that it should be in working order. We will
arrange packing, shipping, etc.

Please reply off line (r.cross-at-ru.ac.za).



=====================================

Rob Cross
Director : EM Unit, Rhodes University


From daemon Thu Nov 28 05:11:46 2002



From: Michael Censlive :      m.censlive-at-mdx.ac.uk
Date: Thu, 28 Nov 2002 10:59:43 +0000
Subject: pure carbon

Contents Retrieved from Microscopy Listserver Archives
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many years ago i used to sputter pure carbon using as source
material " vitreous carbon "
i dont know if its still around but measurments indicated
it was very pure
rgds
M.CENSLIVE
MIDDLESEX UNIVERSITY
ENGINEERING SYSTEMS GROUP
BOUNDS GREEN ROAD
LONDON N11 2NQ
ENGLAND
phone
fax +44 (0) 20 8 362 5210

TEL. + 44 (0)20 8 362 5215
FAX. + 44 (0)20 8 361 1726
E-MAIL MICHAEL56-at-mdx.ac.uk


From daemon Thu Nov 28 07:40:09 2002



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Thu, 28 Nov 2002 08:28:28 -0500
Subject: Ultra cut E

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,
You have heard it partly correct. Yes, Leica is now your contact for the
Reichert UltraCut E. I would suggest you call Philip Hyam (Product and
Marketing Manager) 1-800-205-3422. As for spare parts, bulbs for example are
readily available, one source being Microlites, 2370 Midland Ave.
Scarborough, ON, 416-299-5301.

Regards,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com


-----Original Message-----
} From: "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com
[mailto:"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com]
Sent: Wednesday, November 27, 2002 2:19 PM
To: Microscopy-at-sparc5.microscopy.com


Hi there.
So who is/are the sales reps for Reichert in my neighborhood.
I would like to purchase a couple more chucks for the Ultracut E
Thanks

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



From daemon Fri Nov 29 00:42:05 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 29 Nov 2002 01:16:05 -0500
Subject: EDS of sectioned samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Bill Tivol wrote:
=================================================
On Wednesday, November 27, 2002, at 06:20 AM, John Shields wrote:

} I have several people that wish to look at possible metal uptake in
} bacteria
} and would prefer to enbed them in either Spurr's or Epon, however there
} might be a problem with trace metals in these resins.
} Questions are:
} 1. Is this a real concern? and
} 2. Would another resin be more suitable?
}
} I'm trying to avoid cryo here.
} Thanks

Dear John,
I have looked at metals in embedded tissues, but I have not examined
the same tissues before embedding. I don't think that the metals I
looked at moved around, but they were not very active chemically, so
my experience might not apply to your problem. I would caution you,
however, that EDX is not good for trace metals; the atom fraction has
to be ~0.1% (in the volume where the x-rays are produced), and, if you
are looking for Pb and the bacteria contain S (or other overlapping
lines) you won't see Pb unless you can examine the higher energy Pb
lines. In other words, the resin may be the least of your problems.
=====================================================
If one takes the sections, irrespective of the resin, but we would prefer
GMA because, unlike the epoxies, it does not contain any S or Cl, and puts
them down on a silicon dioxide/monoxide coated (some would say "filmed")
grid, and then subject the now supported (and unstained) section to
literally just a few seconds of oxygen plasma etching such as in the SPI
Plasma Prep II plasma etcher, the embedding resin can be completely removed,
thereby increasing greatly the sensitivity for analysis by EDS. The SiO2
support film, unlike carbon, won't be bothered by the oxygen plasma. Our
impression is that the GMA etches away more cleanly than the epoxies, but
more work should be done to be sure that is indeed the case.

This is sort of hard to explain, but since one picture is still worth 10,000
words, see URL
http://www.2spi.com/catalog/instruments/etchers1.shtml
and specifically, the section "Applications for TEM", specifically "Low
temperature oxygen plasma etched thin section of bacterium embedded in SPI
Chem Low Acid GMA for TEM". I have been told on more than a few occasions
that that approach does enable one to over come the sensitivity issues.

Disclaimer: SPI Supplies manufactures the Plasma Prep II plasma etcher for
this kind of application in the microscopy world. We also produce customer
coated grids that are "filmed" with SiO2, which won't etch away in the
oxygen plasma.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Fri Nov 29 10:25:52 2002



From: =?ISO-8859-2?Q?Old=F8ich_Benada?= :      benada-at-biomed.cas.cz
Date: Fri, 29 Nov 2002 17:12:12 +0100
Subject: Minolta scanner - TEM Negatives

Contents Retrieved from Microscopy Listserver Archives
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Hi,
Does anybody have experiences with Minolta DiMAGE
Scan Multi PRO scanner and TEM negatives (6.5 x 9 cm)
planfilms and glass plates?
Thanks in advance.
Oldrich Benada
+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-241062399
Fax: +420-241062347
WEB: http://www.biomed.cas.cz/mbu/lem113/lem.htm




From daemon Sat Nov 30 14:47:50 2002



From: zaluzec-at-microscopy.com
Date: Sat, 30 Nov 2002 14:12:24 -0600
Subject: Call for Papers: Microscopy & Microanalysis 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues..

The Call for Papers for Microscopy & Microanalysis 2003
in San Antonio Tex, Aug 3-7 2003 is now on-line at

http://www.msa.microscopy.com/MMHomePage.html

Printed copies are currently in the mail to all members of
the sponsoring Societies (MSA, MAS, IMS, CIASEM),
previous meeting attendee's, Exhibitors, etc...


Nestor
Your Friendly Neighborhood SysOp.



From daemon Mon Dec 2 10:09:08 2002



From: Larry Hanke :      hanke-at-mee-inc.com
Date: Mon, 02 Dec 2002 09:45:36 -0600
Subject: Re: cutting very hard materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Buehler has high speed (and slow speed) cutting machines
that can be equipped with diamond wheels for cutting very
hard materials. Look at
http://www.buehlerltd.com/productinfo/precision_saws/Isomet5000.pdf

Disclaimer: I have no commercial interest in Buehler
products.
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870




From daemon Mon Dec 2 13:17:47 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Mon, 02 Dec 2002 14:03:30 -0500
Subject: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
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I received the following email from a friend in DC and don't know how to
help her because I hopelessly burdened with expertise only in high end systems.
My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, but
any suggestions are appreciated.
-----------------
HELP!!!! All Katie wants for Christmas is a good microscope. She wants to
be able to really see things and does not want a "kid" microscope. I need
something with good optics but not expensive. Used is fine. Any ideas,
brand names, places to look? Thanks.
------------------



____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/



From daemon Mon Dec 2 18:05:54 2002



From: Thor Bostrom :      t.bostrom-at-qut.edu.au
Date: Tue, 03 Dec 2002 10:05:27 +1000
Subject: Re: EDS of sectioned samples

Contents Retrieved from Microscopy Listserver Archives
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=================================================
On Wednesday, November 27, 2002, at 06:20 AM, John Shields wrote:
} I have several people that wish to look at possible metal uptake in
} bacteria
} and would prefer to enbed them in either Spurr's or Epon, however there
} might be a problem with trace metals in these resins.
} Questions are:
} 1. Is this a real concern? and
} 2. Would another resin be more suitable?
} I'm trying to avoid cryo here.
} Thanks

Dear John & listers,

With EDX of sections the sensitivity is at best about 0.05--0.1wt% so trace
metals are unlikely to be a problem. Chlorine is detectable in Spurrs but
probably won't interfere with the elements of interest. I have analysed
metals in microalgae, and we did have difficulty seeing significant levels
in the cells in resin sections, and found we had to go to cryosections. The
implication is that a lot of the metals were lost during processing, so one
has to be careful with the procedure adopted. There were also insoluble
granules in the cells that appear to have fallen out from the thin resin
sections. If it's at all possible, it would be a good idea to do a chemical
analysis of a batch following metal uptake to check that the metals are
indeed incorporated into (or on) the cells, so that you have some idea of
what levels to expect and whether you might have lost some during processing.

With regards,
Thor



=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Dr Thor Bostrom
Acting Director,
Analytical EM Facility,
Faculty of Science,
Queensland University of Technology (QUT)
GPO Box 2434, Brisbane, QLD 4001, Australia
Ph: +61 7 3864-2351 FAX: +61 7 3864-5100
http://www.sci.qut.edu.au/aemf/
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=



From daemon Mon Dec 2 19:15:02 2002



From: vladig-at-tht.net (by way of MicroscopyListserver)
Date: Mon, 2 Dec 2002 19:06:21 -0600
Subject: Ask-A-Microscopist: etchant

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (vladig-at-tht.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
December 2, 2002 at 16:09:33
---------------------------------------------------------------------------

Email: vladig-at-tht.net
Name: Vladimir Igoshev

Organization: RIM

Education: Graduate College

Location: City, State, Country

Question: Dear All,

Does anybody know (can share) which etchant should be used for
Electroless Nickel plating?

Thank you in advance for your input.

Vladimir Igoshev, Ph.D.
Toronto, Canada



---------------------------------------------------------------------------


From daemon Mon Dec 2 22:40:15 2002



From: Gordon Nord :      gnord-at-mindspring.com
Date: Mon, 2 Dec 2002 23:26:21 -0500
Subject: Re: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael,

I had this dilemma a few years ago. The solution is not a microscope
(expensive and not portable) but a good wide field hand lense. She can
examine things and carry the hand lense around. It is perfect for the
beginner.

You can visit {http://www.edmundoptics.com} and look at the magnifiers.
For $50 she can get a 12x magnifier.

On Monday, December 2, 2002, at 02:03 PM, Michael Cammer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I received the following email from a friend in DC and don't know how
} to help her because I hopelessly burdened with expertise only in high
} end systems.
} My thoughts have been Edmunds Scientific, the Fisher catalog or eBay,
} but any suggestions are appreciated.
} -----------------
} HELP!!!! All Katie wants for Christmas is a good microscope. She wants
} to
} be able to really see things and does not want a "kid" microscope. I
} need
} something with good optics but not expensive. Used is fine. Any ideas,
} brand names, places to look? Thanks.
} ------------------
}
}
}
} ____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of
} Med.
} Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY
} 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
}
}
}
Dr. Gordon Nord
Senior Scientist
Environmental Sciences Laboratory
Brooklyn College
Brooklyn NY 11210



From daemon Tue Dec 3 03:33:24 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 03 Dec 2002 01:20:09 -0800
Subject: Re: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael

If you really looking for microscope, the Russian microscopes may be a
solution. I do know that LOMO is manufactured and distributed in US very
cheap decent optical microscopes from very basic to serious models. Those
microscopes are utilized old-fashion Carl-Zeiss German tradition, so they
are really good if you not looking for sophistication. You may try the
following link
http://www.lomoplc.com/index2.html but I am pretty sure you may find more.

Best wishes, Sergey.

At 08:26 PM 12/2/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Dec 3 08:55:26 2002



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Tue, 3 Dec 2002 09:55:10 -0500
Subject: Cleaning ATW Window on Oxford X-ray Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers,

Can anyone suggest a SAFE procedure for removing oil deposits from the
surface of the ATW2 (light element) window on my Oxford detector? While
we're at it, does anyone know of a process for cleaning/polishing the
bottom of the inside of the dewar on the same detector. I looked inside
and it appears to have a layer of deposits (dirt?) on the bottom. Might
this be contributing to high LN2 consumption? TIA and MC and HNY. Bill
Roberts



This communication is for use by the intended recipient and contains
information that may be privileged, confidential or copyrighted under
applicable law. If you are not the intended recipient, you are hereby
formally notified that any use, copying or distribution of this e-mail,
in whole or in part, is strictly prohibited. Please notify the sender
by return e-mail and delete this e-mail from your system. Unless
explicitly and conspicuously designated as "E-Contract Intended",
this e-mail does not constitute a contract offer, a contract amendment,
or an acceptance of a contract offer. This e-mail does not constitute
a consent to the use of sender's contact information for direct marketing
purposes or for transfers of data to third parties.

Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean

http://www.DuPont.com/corp/email_disclaimer.html




From daemon Tue Dec 3 08:55:27 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 03 Dec 2002 09:44:12 -0500
Subject: thanks: kid's microscope suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many of you sent excellent suggestions for kids' microscopes. Thanks!
We'll let you know what they pick, and some of the suggestions look good
for my kids too.
Thanks!

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/




From daemon Tue Dec 3 09:22:04 2002



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Tue, 3 Dec 2002 10:31:26 -0500
Subject: LM, Digital Imaging workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The New York Microscopical Society has available to members two microscopes
which are of very good quality and quite inexpensive.

We have a 20X dissecting microscope at $90 (members price $ 55) and a
Compound microscope with 4X, 10X & 40X Society thread objectives and a built
in light source at $225 (members price $155).

These are the same microscopes we use for our classes for children.

Don
----- Original Message -----
} From: "Gordon Nord" {gnord-at-mindspring.com}
To: "Michael Cammer" {cammer-at-aecom.yu.edu}
Cc: "Microscopy List" {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, December 02, 2002 11:26 PM


New York Microscopical Society

30 North Mountain Avenue

Montclair, NJ 07042








Bernard Friedman


Memorial Workshop






Digital Image Capture and Management in Microscopy

May 9, 2003


A course on Digital imaging in light microscopy which will cover the
following topics:


Optical Limitations in Light Microscopy...Photographic Imaging Strategies...
Digital Imaging Strategies...Selection of Digital Capture (Camera vs.
Scanner)...

Image Processing of Captured Images...Image File Formats...Printing
Images... Color Management Systems...Database Management
Software...Presentation Software for Oral Reports...Website
Performance...Integration of Image Data with Sample Information,
Calibration, Other Data & Reports...Acrobat and html Software for Written
Reports and Archives...Examples of Efficient, Low Cost Image Handling
Systems...

Examples of Electronic Microscopy Reports and Databases


The course instructors are Mary and John McCann of McCann Imaging.

WHEN: Friday, May 9, 2002, from 9 A.M. to 5 P.M.

WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043

COST: $350 for N.Y.M.S. members, $380 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

HOW: Register using the form below. Limited to the first 12 registrants.

Return form with a check to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ
07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 e-mail
donoleary-at-att.net

PLEASE POST

----------------------------------------------------------------------------
------------------------------------------------

Digital Image Capture Registration Form

N.Y.M.S. Member_________________ ($350) Non-Member__________($380)

Name____________________________________________________________________

Address__________________________________________________________________

Phone (W)_____________________(H)_____________________Fax_________________

e-mail________________________________________



From daemon Tue Dec 3 09:51:19 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 3 Dec 2002 07:37:06 -0800
Subject: Re: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I received the following email from a friend in DC and don't know how to
} help her because I hopelessly burdened with expertise only in high end
} systems.
} My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, but
} any suggestions are appreciated.
} -----------------
} HELP!!!! All Katie wants for Christmas is a good microscope. She wants to
} be able to really see things and does not want a "kid" microscope. I need
} something with good optics but not expensive. Used is fine. Any ideas,
} brand names, places to look? Thanks.
} ------------------
Michael Cammer

Michael -

First, your friend should look at the microscope-buying advice on the MICRO
website. The first decision is type; dissecting vs. compound - both have
advantages. Sources are suggested on the website, but there isn't much
shopping time; my personal favorite place for good onscreen advice and fair
prices is www.microscopeworld.com. I'd avoid eBay for a first scope; a
used one may have faults, and it's likely to be too complex as well. Both
will frustrate a youngster.

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Dec 3 10:19:45 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 3 Dec 2002 11:10:40 -0500
Subject: Ask-A-Microscopist: etchant

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Vladimir;

Try Acton Technologies, Pennsylvania, USA for a Ni etch,
http://www.actontech.com/elec1.htm.

I've used their materials and they generally work as advertised. I have no
interest in the company other than as a user. They send me no holiday
gifts.

Regards,

Peter Tomic
Anadigics,, Inc.
Warren, New Jersey
USA

-----Original Message-----
} From: vladig-at-tht.net [mailto:vladig-at-tht.net]
Sent: Monday, December 02, 2002 8:06 PM
To: Microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (vladig-at-tht.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
December 2, 2002 at 16:09:33
---------------------------------------------------------------------------

Email: vladig-at-tht.net
Name: Vladimir Igoshev

Organization: RIM

Education: Graduate College

Location: City, State, Country

Question: Dear All,

Does anybody know (can share) which etchant should be used for
Electroless Nickel plating?

Thank you in advance for your input.

Vladimir Igoshev, Ph.D.
Toronto, Canada



---------------------------------------------------------------------------


From daemon Tue Dec 3 10:40:03 2002



From: Larry Hanke :      hanke-at-mee-inc.com
Date: Tue, 03 Dec 2002 10:31:16 -0600
Subject: Re: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have found that the things middle schoolers like to look
at can be best observed with a good stereo microscope. A
hand lens is great, but a stereo microscope with a light
will be cooler to 5th grader.

In my town, we have several used scientific equipment
dealers that peddle pretty decent used scopes for a
reasonable price - better value for the money buying used
Bausch and Lomb, Olympus or Nikon than a new cheap brand. It
may take some hunting around to find these dealers. Ebay is
another alternative. Make sure that the unit is a stereo
microscope with two eyepieces.

Good luck.
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870




From daemon Tue Dec 3 11:21:47 2002



From: John W. Raffensperger, Jr. :      johnr-at-helwigcp.com
Date: Tue, 3 Dec 2002 11:45:07 -0600
Subject: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Vladimir,
My Vander Voort mentions two solutions for electroless nickel:
Formula 1:
45 g nickel chloride
11 g sodium hypophosphite
100 g sodium citrate
50 g ammonium chloride
1000 ml. water
pH 8.5-9, use a 194-212 °F. Plating rate: 0.015 mm/h

Formula 2:
37.3 g nickelous sulfate
26.4 g sodium hypophosphite
15.9 g sodium acetate
5-6 drops sulfuric acid
1000 ml. water
Use at 180-190 °F, plating rate 0.01 mm/h

I hope this helps.

----- Original Message -----
} From: "by way of MicroscopyListserver" {vladig-at-tht.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, December 02, 2002 5:06 PM


Two other sources similar to Edmund Scientific are:

Carolina Biological Supply http://www.carolina.com/
Nasco http://www.enasco.com/prod/Home

Both are primarily aimed at school supply, but also do retail sales.

They have a full range of instruments from simple hand lenses, portable
hand held "field microscopes" and beginning through "advanced" compound
microscopes. Some of the more advanced scopes include a photo tube.
They stop short of the real high end (Nikon, Olympus, etc.)

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Michael Cammer [mailto:cammer-at-aecom.yu.edu]
Sent: Monday, December 02, 2002 1:04 PM
To: Microscopy-at-sparc5.microscopy.com


I received the following email from a friend in DC and don't know how to

help her because I hopelessly burdened with expertise only in high end
systems.
My thoughts have been Edmunds Scientific, the Fisher catalog or eBay,
but
any suggestions are appreciated.
-----------------
HELP!!!! All Katie wants for Christmas is a good microscope. She wants
to
be able to really see things and does not want a "kid" microscope. I
need
something with good optics but not expensive. Used is fine. Any ideas,
brand names, places to look? Thanks.
------------------



________________________________________________________________________
____
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of
Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY
10461
(718) 430-2890 Fax: 430-8996 URL:
http://www.aecom.yu.edu/aif/



From daemon Tue Dec 3 12:45:43 2002



From: Judy Trogadis :      TrogadisJ-at-smh.toronto.on.ca
Date: Tue, 03 Dec 2002 13:32:21 -0500
Subject: image handling software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists :

I recently collected a lot of images at a single sitting, each image seemingly better than the previous one, definitely on a roll. However, the next day I laboriously had to review a lot of images of similar data and choose the best ones.

What software are people using to browse through a lot of images, as thumbnails, easily change their names, perhaps add annotations, move selected ones into new folders and create a catalogue. It should also be able to recognize and load confocal image stacks.

Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Queen
30 Bond St.
Toronto, ON M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
pager: 416-685-9219
trogadisj-at-smh.toronto.on.ca




From daemon Tue Dec 3 13:30:00 2002



From: Kai Lorcharoensery :      kai-at-lehigh.edu
Date: Tue, 03 Dec 2002 14:19:22 -0500
Subject: Re: Ask-A-Microscopist: etchant

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am afraid that the given formula are for preparing the plating
solution. The etchant that I tried consists of: 1 part of water, 1 part
of acetic acid, and 2 parts of nitric acid. All are in volume ratio.
This etchant works very fast. Dip a few seconds and see the result
before proceeding. It reveals grain boundaries and striations of
phosphorus-depleting layers. A word of caution, it can attacks the
substrate violently such that you get a good coating microstructure but
not the substrate.

In their book, Rostoker & Dvorak used 5 gram of CrO3 in 100 ml of water
as a solution for electrolytic etching. This one reveals both
P-striations and Ni3P (if present).

The most important thing is safety. Both HNO3 and CrO3 are strong
oxidizer. CrO3 will turn to acid upon dissolving, and it is carcinogen.
Please read MSDS before using them.

Kai Lorcharoensery
Materials Science & Engineering
Lehigh University
Bethlehem, PA

Mary Mager wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Vladimir,
} My Vander Voort mentions two solutions for electroless nickel:
} Formula 1:
} 45 g nickel chloride
} 11 g sodium hypophosphite
} 100 g sodium citrate
} 50 g ammonium chloride
} 1000 ml. water
} pH 8.5-9, use a 194-212 °F. Plating rate: 0.015 mm/h
}
} Formula 2:
} 37.3 g nickelous sulfate
} 26.4 g sodium hypophosphite
} 15.9 g sodium acetate
} 5-6 drops sulfuric acid
} 1000 ml. water
} Use at 180-190 °F, plating rate 0.01 mm/h
}
} I hope this helps.
}
} ----- Original Message -----
}
} } From: "by way of MicroscopyListserver" {vladig-at-tht.net}
}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Monday, December 02, 2002 5:06 PM
} Subject: Ask-A-Microscopist: etchant
}
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Tue Dec 3 13:51:05 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 03 Dec 2002 14:42:34 -0500
Subject: Re: Cleaning ATW Window on Oxford X-ray Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill-

I'm not sure there is a SAFE procedure for cleaning your window. Are you
sure it is contaminated? (If the answer is yes, then the rest of this
e-mail is irrelevant).

If you have high LN2 consumption on a detector that used to be good then
almost certainly you have poor vacuum (I can't see why the crud inside the
dewar would contribute to that - I have seen such deposits in the dewars of
my detectors when they have been working fine - I've no idea what it
is). With poor vacuum you would almost certainly have detector
icing. (Have you run your detector conditioner?) Icing would lead to loss
of sensitivity to low energy X-rays. A solution (that might only be
temporary, if you have a significant leak) would be to warm the detector
and pump it.

Having said all the above, we have 4 EDX detectors in use, the LN2
consumption varies widely, the most complex (a behemoth double gate-valve
windowless on a VG STEM) has the lowest consumption of the 4, the simplest
(on our JEOL 2010) has by far the highest consumption of any detector I've
ever encountered, but it has always been that way (it was so bad we had it
checked out by the manufacturer), and has always (and continues to) work
just fine. Black magic, I suppose!

Tony.

At 09:55 AM 12/3/2002 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Tue Dec 3 14:06:52 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Tue, 3 Dec 2002 13:58:28 -0600
Subject: RE: Cleaning ATW Window on Oxford X-ray Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I clean my detectors (PGT) with Vertrel XF solvent recommended by
PGT (see, for example, http://www.miller-stephenson.com/release_006.htm)
Procedure:
Remove collimator.
Place detector so that window is vertical (if necessary, adjust
its position later).
From a pipette put a few drops of Vertrel on a top metal ring
surrounding window. In no case should a single drop be placed
directly on the window. See if liquid is running along window surface.

Good luck,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


}
} Greetings Listers,
}
} Can anyone suggest a SAFE procedure for removing oil deposits from the
} surface of the ATW2 (light element) window on my Oxford
} detector? While
} we're at it, does anyone know of a process for cleaning/polishing the
} bottom of the inside of the dewar on the same detector. I
} looked inside
} and it appears to have a layer of deposits (dirt?) on the
} bottom. Might
} this be contributing to high LN2 consumption? TIA and MC and
} HNY. Bill
} Roberts
}
}
}
} This communication is for use by the intended recipient and contains
} information that may be privileged, confidential or copyrighted under
} applicable law. If you are not the intended recipient, you are hereby
} formally notified that any use, copying or distribution of
} this e-mail,
} in whole or in part, is strictly prohibited. Please notify the sender
} by return e-mail and delete this e-mail from your system. Unless
} explicitly and conspicuously designated as "E-Contract Intended",
} this e-mail does not constitute a contract offer, a contract
} amendment,
} or an acceptance of a contract offer. This e-mail does not constitute
} a consent to the use of sender's contact information for
} direct marketing
} purposes or for transfers of data to third parties.
}
} Francais Deutsch Italiano Espanol Portugues Japanese
} Chinese Korean
}
} http://www.DuPont.com/corp/email_disclaimer.html
}
}
}
}


From daemon Tue Dec 3 14:40:04 2002



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Tue, 3 Dec 2002 14:38:32 -0600 (CST)
Subject: Antivibration systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate comments from USERS about pro's and
con's of different antivibration manufacturers systems.

-----------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2225 N Campus Drive
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
-----------------------------------------------




From daemon Tue Dec 3 16:56:41 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 3 Dec 2002 22:27:49 -0500
Subject: Cleaning ATW Window on Oxford X-ray Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Judy,

I don't know about loading confocal image stacks, but Irfanview, a freeware
product for PC (www.irfanview.com) allows you to rapidly flip through a
large number of images, and recognizes all the usual formats.

If all the images are in a single directory you can view them sequentially
by hitting space bar. It's the best I've found yet if you want to get a
quick overview with minimum time and effort invested.

Wharton Sinkler
UOP LLC
Des Plaines, IL

-----Original Message-----
} From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca]
Sent: Tuesday, December 03, 2002 12:32 PM
To: Microscopy-at-sparc5.microscopy.com



I very gently dribble Freon (yes, I still have a very little left) over the window, but I'm
talking Be.

You should ask Oxford for their recommendations.

I have cleaned out my dewar by swirling methanol around in it, but of course you need
to take it off your SEM for that.
I understand that dirt in the dewar can increase noise, by providing nucleii for LN2
boiling, but my (limited) understanding is that high LN2 consumption is more likely to be
caused by a deteriorated vacuum in the dewar, which really needs a pump/bake to fix it.
I've just had that done to my PGT detector, improved the LN2 consumption remarkably
and also resulted in the detector crystal running a few degrees colder, which should
improve the noise performance but doesn't seem to have.

There's also the possibility of a slow leak in the window.

You should maybe ask Oxford also about recooling it, if you don't have a detector
reconditioner built in to your interface there's a scary procedure involving boiling water
which, I believe, drives adsorbed stuff off the crystal onto the getter.

cheers

rtch




Bill;

I have not tried this myself but did observe our service engineer clean the
window on a 6 uM thick Be SUTW window. What he did was remove the dewar,
detector housing and nosepiece from the electron column. With the housing
held firmly, he ran droplets of acetone from a squeeze bottle down the face
of the detector until it appeared clean. He DID NOT wipe the detector face,
and that's important since even the slight pressure of a cotton swab may
fracture it. Having told you all of this, I would still suggest asking the
manufacturer [Oxford] whether they have a recommended cleaning method or
would approve of what I just stated. Be certain that the column pressure is
at atmosphere prior to attempting to remove the detector. Sudden changes in
pressure can and do fracture these very thin EDX windows and that's an
expensive mistake. I assume you have a diffusion pumped SEM because of the
oil and not a turbomolecular pump OR extremely dirty specimens.

With regard to getting dirt out of the dewar, you may want to hold it upside
down and blow it out with nitrogen at a low pressure. Of course it needs to
be dry at the time. I doubt whether dirt would be affecting your LN2
consumption since it's principally evaporation that causes it do disappear
and that's through the fill hole. Whomever is filling the dewar may not
actually be topping it off and maybe it just appears to require filling more
often. Of course when it's being filled and it's at room temp. the LN2 will
boil and generate gas which will impede the filling process. This is
remedied by simply filling the dewar partially and allowing it to cool and
then topping it off. We have a 7 quart dewar and that generally is good for
7 days. If you are already doing all of this and still find yourself
filling the dewar frequently, you may want to inspect it for a breach in the
dewar wall itself. It should be evacuated between the inner and outer walls
with no leaks.

I hope this is of some assistance.

Peter Tomic
Anadigics, Inc.



-----Original Message-----
} From: William H Roberts [mailto:William.H.Roberts-at-usa.dupont.com]
Sent: Tuesday, December 03, 2002 9:55 AM
To: Microscopy-at-sparc5.microscopy.com


Greetings Listers,

Can anyone suggest a SAFE procedure for removing oil deposits from the
surface of the ATW2 (light element) window on my Oxford detector? While
we're at it, does anyone know of a process for cleaning/polishing the
bottom of the inside of the dewar on the same detector. I looked inside
and it appears to have a layer of deposits (dirt?) on the bottom. Might
this be contributing to high LN2 consumption? TIA and MC and HNY. Bill
Roberts



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From daemon Tue Dec 3 22:17:09 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Tue, 03 Dec 2002 23:21:57 -0500
Subject: Re: Ask-A-Microscopist: etchant

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've had pretty good results with 10% ammonium persulfate (swabbing). This has
the advantage that it isn't uncontrollably aggressive to base metals.

If health and safety considerations are met, dilute potassium cyanide /
hydrogen peroxide (typically 5% of each mixed immediately before use and
applied by swabbing ) performs well. This is particularly useful for gold over
electroless nickel, as it simultaneously develops the structure of both metals
and allows thickness measurements to be made that are not influenced by
smearing of the gold.

John Twilley
Conservation Scientist

by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (vladig-at-tht.net) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} December 2, 2002 at 16:09:33
} ---------------------------------------------------------------------------
}
} Email: vladig-at-tht.net
} Name: Vladimir Igoshev
}
} Organization: RIM
}
} Education: Graduate College
}
} Location: City, State, Country
}
} Question: Dear All,
}
} Does anybody know (can share) which etchant should be used for
} Electroless Nickel plating?
}
} Thank you in advance for your input.
}
} Vladimir Igoshev, Ph.D.
} Toronto, Canada
}
} ---------------------------------------------------------------------------





From daemon Tue Dec 3 23:52:09 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 4 Dec 2002 08:59:40 +0100
Subject: Re: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bill and Listers,



The traditional way of cleaning windows when I worked at Kevex 20 year
ago was to run IPA over the window. The IPA was poured onto the metal
cylindrical snout area above and away from the window and the IPA
would flow over the window and into a beaker below. One did not
direct IPA directly at the window. Most of our FS engineers would
remove the detector from the chamber before doing this. I did it
couple of times with it mounted. It was very tricky. Most FS
engineers broke one or two windows in their careers so it wasn't that
"Safe".



The reason for high LN consumption is a poor vacuum in the dewar. A
dewar with a UTW needs to be baked and repumped every 5 years or so.
The dirt at the bottom of the LN container does not make much
difference. The poor vacuum also causes the snout to be cold which
causes the window to collect oil. Send the dewar back to Oxford or a
qualified service organization for dewar pumping and baking if you are
not equipped to do this yourself.



Commercial Message: The Evactron anti-contaminator for removing oil
and contamination from electron microscopes will clean your X-ray
window safely and keep it from coming back. It combines RF plasma
cleaning with Nitrogen purging to clean and remove oil form SEM
chambers and EDS windows. More information is at www.SEMCLEAN.COM.



Notice: I invented the Evactron Anti-contaminator to solve oil
problems and have an interest in selling more. Evactron is my
registered trademark.



Ronald Vane

President

XEI Scientific

3124 Wessex Way

Redwood City, CA 94061

650-369-0133

650-363-1659



----- Original Message -----
} From: "William H Roberts" {William.H.Roberts-at-usa.dupont.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, December 03, 2002 6:55 AM



Larry Hanke wrote :

{ {I have found that the things middle schoolers like to look
{ {at can be best observed with a good stereo microscope. A
{ {hand lens is great, but a stereo microscope with a light
{ {will be cooler to 5th grader.

And if you can find a TEM focusing binocular, it's a very good tool. Long
working distance, x10 magnification, large objective diameter, that means
much light. The child can use it outdoor with sun light, or home with a
halogen desk light. And most of it are high quality optics. I have two
from EM 300 TEM, one on a stand, the other for use in hand, and my
children have enjoyed with it.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France






From daemon Wed Dec 4 02:24:50 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 4 Dec 2002 09:16:25 +0100
Subject: etchant for PtFeCo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does some know a solution to etch a serie of PtFeCo alloys. They
have 75% or 50% (weight) Pt and go from 0% Co - 100% Fe to the opposit. We
want to mesure the grain size. I have some receipt for Pt or for FeCo
alloys, but nothing for that situation, with a mixing of noble metal and
FeCo.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Wed Dec 4 04:12:55 2002



From: Qian-chun Yu, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Wed, 04 Dec 2002 05:04:10 -0500
Subject: Service fee structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

We are trying to establish a reasonable service fee structure for our
multi-user Core Facility, and would appreciate your input or information.

This is a basic fee structure for some of the common procedures performed
at a biomedical shared core facility such as TEM (regular thin section as
well as negative stain), SEM, immoEM, confocal, multiphoton microscopy, and
broadband fluorescence microscopy. We generally do not cover material
science projects.

The fee covers only the research-based activities at a private university
with no involvement of clinical service. It should cover the personnel time
and effort, materials required, as well as the facility use; but NO PROFIT
is permitted.

I believe many of us are, or will be, involved in this issue. Any input,
guidelines, suggestions, or special concerns are all welcome. Please feel
free to contact me off-line if you prefer. Thank you for any help, and
wish you a wonderful holiday season!

QC Yu

======================================
Qian-Chun Yu, MB, Ph.D.
Director
Biomedical Imaging Core Laboratory
University of Pennsylvania
School of Medicine
110 Richards Building
Philadelphia, PA 19104

Phone: 215-573-7766 (Voicemail)
FAX: (215)573-2259
E-mail: qcyu-at-mail.med.upenn.edu




From daemon Wed Dec 4 08:06:10 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Wed, 4 Dec 2002 05:57:21 -0800 (PST)
Subject: Re: image handling software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Judy:

There are a number of outfits that sell software (and
hardware) for applications such as you described.
It's becoming a growing and competitive product in the
microscopic imaging industry.

Vital Image Technology
Scott Taylor
Regional Manager
450 Portage Trail
Cuyahoga Falls, Ohio 44221
ph. 330-940-3200
fax. 330-940-3222
st-at-vitalimage.com

Mager Scientific, Inc.
Jim Uren
PO Box 160
Dexter, Michigan 48310-0160
ph. 734-426-3885
fax. 734-426-3987
voice. 734-426-1116
juren-at-magersci.com

Either of them would be more than happy to send you
literature describing the capabilities of their
products.

Stu Smalinskas
SKF USA
Plymouth, Michigan


{Judy wrote:
{
{Fellow microscopists:
{
{I recently collected a lot of images at a single
{sitting, each image seemingly better than the
{previous one, definitely on a roll. However, the next
{day I laboriously had to review a lot of images of
{similar data and choose the best ones.
{
{What software are people using to browse through a
{lot of images, as thumbnails, easily change their
{names, perhaps add annotations, move selected ones
{into new folders and create a catalogue. It should
{also be able to recognize and load confocal image
{stacks.
{
{Thank you
{Judy
{
{Judy Trogadis
{Bio-Imaging Coordinator
{St. Michael's Hospital, 8Queen
{30 Bond St.
{Toronto, ON M5B 1W8
{Canada
{
{ph: 416-864-6060 x6337
{fax: 416-864-6043
{pager: 416-685-9219
{trogadisj-at-smh.toronto.on.ca


__________________________________________________
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Yahoo! Mail Plus - Powerful. Affordable. Sign up now.
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From daemon Wed Dec 4 09:05:06 2002



From: Peggy Miller :      millermm-at-uthscsa.edu
Date: Wed, 04 Dec 2002 08:57:19 -0600
Subject: FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

An assossiate is looking for a facility with FESEM capabilities for nano
materials research. Please contact Erin at emclaughlin-at-tritonsystems.com

Peggy Miller
UTHSCSA
Department of Ophthalmology
Lions Sight Research Foundation
Ph: (210) 567-8460
Fax: (210) 567-8413



From daemon Wed Dec 4 09:11:38 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Wed, 4 Dec 2002 10:04:35 -0500
Subject: Looking for JEOL 100S viewing screen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone out there have a viewing screen for a JEOL 100S they no longer
need or are willing to part with? Quailty of the phosphor is not critical (we'll
get it re-coated) but the qulaity of the aluminum backing IS important.

Figured its worth a shot.

Thanks



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Wed Dec 4 10:38:29 2002



From: Qian-Chun Yu, MB, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Wed, 04 Dec 2002 11:21:53 -0500
Subject: Environmental SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

Does anyone know an environmental SEM facility located in the Baltimore-DC
area? A friend of mine wanted to analyze samples without dehydration. I
remember someone mentioned the environmental SEM, but did not take note.

Thanks for any information.

QC Yu



From daemon Wed Dec 4 10:52:40 2002



From: Peggy Miller :      millermm-at-uthscsa.edu
Date: Wed, 04 Dec 2002 10:45:08 -0600
Subject: FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

An assossiate is looking for a facility with FESEM capabilities for nano
materials research. Please contact Erin at emclaughlin-at-tritonsystems.com if
you can be of assistance.

Best Regards,
Peggy Miller
UTHSCSA
Department of Ophthalmology
Lions Sight Research Foundation
Ph: (210) 567-8460
Fax: (210) 567-8413



From daemon Wed Dec 4 11:34:46 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Wed, 04 Dec 2002 09:25:41 -0800
Subject: Re: etchant for PtFeCo - Metallographic Etch Database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Faerber:

You may want to check our Metallographic Etch Database which you can find a
link to on our website. Go to www.southbaytech.com and enter MED-1 in the
keyword box in the upper right corner. If you open the PDF data sheet it will
give you a link to a demo version of the database which will allow you to
search by material for an etchant.

I hope this helps.

DISCLAIMER: South Bay Technology produces equipment and supplies as described
above and, therefore, has a vested interest in promoting their use.

Best regards-

David

Faerber Jacques wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Does some know a solution to etch a serie of PtFeCo alloys. They
} have 75% or 50% (weight) Pt and go from 0% Co - 100% Fe to the opposit. We
} want to mesure the grain size. I have some receipt for Pt or for FeCo
} alloys, but nothing for that situation, with a mixing of noble metal and
} FeCo.
}
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces)
} 23, rue de Loess ; BP43
} 67034 Strasbourg CEDEX 2
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)3 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

--
David Henriks
Vice President

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 38 years of providing Materials Processing Solutions for
Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged
and confidential. This message is intended for the individual or entity
addressed.
If you are not the intended recipient, please do not read, copy or disclose
this communication. Notify the sender of the mistake by calling
+1-949-492-2600 and
delete this message from your system.





From daemon Wed Dec 4 12:42:05 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 4 Dec 2002 09:00:35 -0800
Subject: image handling software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ACDC!

John Mardinly
Desk: 408-765-2346
Pager: 877-277-1182





-----Original Message-----
} From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca]
Sent: Tuesday, December 03, 2002 10:32 AM
To: Microscopy-at-sparc5.microscopy.com


Fellow microscopists :

I recently collected a lot of images at a single sitting, each image seemingly better than the previous one, definitely on a roll. However, the next day I laboriously had to review a lot of images of similar data and choose the best ones.

What software are people using to browse through a lot of images, as thumbnails, easily change their names, perhaps add annotations, move selected ones into new folders and create a catalogue. It should also be able to recognize and load confocal image stacks.

Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Queen
30 Bond St.
Toronto, ON M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
pager: 416-685-9219
trogadisj-at-smh.toronto.on.ca




From daemon Wed Dec 4 13:25:18 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 05 Dec 2002 08:15:20 +1300
Subject: Re: image handling software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} What software are people using to browse through a lot of images, as
} thumbnails, easily change their names, perhaps add annotations, move
} selected ones into new folders and create a catalogue. It should also
} be able to recognize and load confocal image stacks.
}


IrfanView (free from Irfanview.com) has the great property that if you have one image
open, there are two toolbuttons which enable you, with one mouseclick, to open the
next or the previous image in the directory.

Thumbsplus, which I got free with a magazine, has a good thumbnail image viewing
and title-filing system.

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Dec 4 14:31:15 2002



From: tartenon-at-netscape.net
Date: Wed, 04 Dec 2002 15:21:47 -0500
Subject: RE: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A good idea is YS100 from Nikon, it cost around $ 1,400.00 website www.nikonusa.com


Michael Cammer {cammer-at-aecom.yu.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I received the following email from a friend in DC and don't know how to
} help her because I hopelessly burdened with expertise only in high end systems.
} My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, but
} any suggestions are appreciated.
} -----------------
} HELP!!!! All Katie wants for Christmas is a good microscope. She wants to
} be able to really see things and does not want a "kid" microscope. I need
} something with good optics but not expensive. Used is fine. Any ideas,
} brand names, places to look? Thanks.
} ------------------
}
}
}
} ____________________________________________________________________________
} Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus      1300 Morris Park Ave.     Bronx, NY  10461
} (718) 430-2890       Fax:  430-8996      URL:  http://www.aecom.yu.edu/aif/
}
}
}

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From daemon Wed Dec 4 14:51:20 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 04 Dec 2002 15:44:12 -0500
Subject: Re: Service fee structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


QC
We went through revising rates for a multi-user and service facility
just last year. First thing you have to know is actual cost based on time,
materials, salaries, hard costs (such as service contracts), etc. Based on
the number of procedures and hours each piece of equipment has been used,
you can figure past costs and make projections as to future costs. Then you
need to know how much support you will get to subsidize costs and
procedures. Once all this data is known, you can determine final costs for
each procedure or equipment by balancing anticipated actual income vs. funds
for subsidizing.

For example:
An SEM is used 400 hrs/year with a $14,000 service contract + $1000 supplies
(nitrogen, etc). Actual cost per hour is $37.50. But perhaps you have a
TEM that is only used 200 hr/year with the same cost so actual cost is
$75/hr. You may be able to subsidize (at least initially if you are
building a user base) the TEM rate to make it also $37.50/hr which may be
all your researchers can afford to pay. If you have to figure salaries in
than the cost will obviously go much higher.

In my facility we do not have to raise funds to cover salaries. We also
have an ample budget to cover service contracts and other costs at the
present rate. However we must bring in sufficient revenue to cover all
increased costs plus materials and new equipment not obtained on grants.
Therefore we can subsidize rates heavily for internal users (external pay
full cost) and so can keep rates for use and for service quite low. An
example: $11/hr for SEM and TEM use by trained users, $26/hr if technical
help is needed.

Costs for specimen preparation is based on actual anticipated costs of
consumables plus $15/hr technical charge. Consumables, such as film, are
priced at replacement cost as we also cannot make a profit. Multi-users
provide most of their own consumables (fixatives, embedding resins,
photographic paper, etc) so we don't have to keep track of all that
stuff...that would be a nightmare.

Hope this helps. Feel free to contact me if you need more information.
Debby


Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907


On 12/4/02 5:04 AM, "Qian-chun Yu, Ph.D." {qcyu-at-mail.med.upenn.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
}
} We are trying to establish a reasonable service fee structure for our
} multi-user Core Facility, and would appreciate your input or information.
}
} This is a basic fee structure for some of the common procedures performed
} at a biomedical shared core facility such as TEM (regular thin section as
} well as negative stain), SEM, immoEM, confocal, multiphoton microscopy, and
} broadband fluorescence microscopy. We generally do not cover material
} science projects.
}
} The fee covers only the research-based activities at a private university
} with no involvement of clinical service. It should cover the personnel time
} and effort, materials required, as well as the facility use; but NO PROFIT
} is permitted.
}
} I believe many of us are, or will be, involved in this issue. Any input,
} guidelines, suggestions, or special concerns are all welcome. Please feel
} free to contact me off-line if you prefer. Thank you for any help, and
} wish you a wonderful holiday season!
}
} QC Yu
}
} ======================================
} Qian-Chun Yu, MB, Ph.D.
} Director
} Biomedical Imaging Core Laboratory
} University of Pennsylvania
} School of Medicine
} 110 Richards Building
} Philadelphia, PA 19104
}
} Phone: 215-573-7766 (Voicemail)
} FAX: (215)573-2259
} E-mail: qcyu-at-mail.med.upenn.edu
}
}
}
}



From daemon Wed Dec 4 14:51:21 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 4 Dec 2002 12:42:41 -0800
Subject: Re: microscope for very bright 4-5th grader?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon;
I would think that a very bright 4-5th grader today would have seen everything that could be seen with a magnifier already. Splurge and get that kid a real microscope! I recall getting a microscope (cheap, but a real one) for my 8th birthday 45 years ago, and look what happened-I'm spending the rest of my life looking through microscopes! MSA is graying, and is in need of fresh blood, so we should all do our part to inspire the next generation.

John Mardinly
Desk: 408-765-2346
Pager: 877-277-1182





-----Original Message-----
} From: Gordon Nord [mailto:gnord-at-mindspring.com]
Sent: Monday, December 02, 2002 8:26 PM
To: Michael Cammer
Cc: Microscopy List


Michael,

I had this dilemma a few years ago. The solution is not a microscope
(expensive and not portable) but a good wide field hand lense. She can
examine things and carry the hand lense around. It is perfect for the
beginner.

You can visit {http://www.edmundoptics.com} and look at the magnifiers.
For $50 she can get a 12x magnifier.

On Monday, December 2, 2002, at 02:03 PM, Michael Cammer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I received the following email from a friend in DC and don't know how
} to help her because I hopelessly burdened with expertise only in high
} end systems.
} My thoughts have been Edmunds Scientific, the Fisher catalog or eBay,
} but any suggestions are appreciated.
} -----------------
} HELP!!!! All Katie wants for Christmas is a good microscope. She wants
} to
} be able to really see things and does not want a "kid" microscope. I
} need
} something with good optics but not expensive. Used is fine. Any ideas,
} brand names, places to look? Thanks.
} ------------------
}
}
}
} ____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of
} Med.
} Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY
} 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
}
}
}
Dr. Gordon Nord
Senior Scientist
Environmental Sciences Laboratory
Brooklyn College
Brooklyn NY 11210



From daemon Wed Dec 4 17:31:05 2002



From: Poirier, Glenn :      glpoirie-at-nrcan.gc.ca
Date: Wed, 4 Dec 2002 18:21:14 -0500
Subject: image handling software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Judy,
You may also want to check out XnView. It's open source software so
it's free. It does everything ACDSee does, including printing out contact
sheets. I also use ACDSee because its tightly integrated to Windows. The
only problem with XNView is that the installation requires a bunch of
libraries be installed first. As for viewing confocal stacks, you might want
to check out ImageJ which is a extremely nice rewrite of NIH Image using
java.


Glenn Poirier tel: (613) 947-9833
Microbeam Specialist FAX: (613) 996-9673
Mining and Mineral Sciences Laboratory 555 Booth St
CANMET Ottawa, ON K1A OG1



-----Original Message-----
} From: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: 04 December, 2002 12:01 PM
To: Judy Trogadis; Microscopy-at-sparc5.microscopy.com


ACDC!

John Mardinly
Desk: 408-765-2346
Pager: 877-277-1182





-----Original Message-----
} From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca]
Sent: Tuesday, December 03, 2002 10:32 AM
To: Microscopy-at-sparc5.microscopy.com


Fellow microscopists :

I recently collected a lot of images at a single sitting, each image
seemingly better than the previous one, definitely on a roll. However, the
next day I laboriously had to review a lot of images of similar data and
choose the best ones.

What software are people using to browse through a lot of images, as
thumbnails, easily change their names, perhaps add annotations, move
selected ones into new folders and create a catalogue. It should also be
able to recognize and load confocal image stacks.

Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Queen
30 Bond St.
Toronto, ON M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
pager: 416-685-9219
trogadisj-at-smh.toronto.on.ca




From daemon Wed Dec 4 19:04:44 2002



From: Martin Ramirez :      ramirez-at-amnh.org
Date: Wed, 04 Dec 2002 20:00:47 -0500
Subject: composite 2-D images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,

Does anyone knows of a free or accessible software to compose in-focus 2-D
images from several images in different focal planes? Martin



Martin J. Ramirez
Division of Invertebrate Zoology
American Museum of Natural History
Central Park West at 79th Street
New York 10024, N.Y.
USA
Tel: (212) 769 5609
Fax: (212) 769 5277
email: ramirez-at-amnh.org



From daemon Wed Dec 4 19:07:49 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Wed, 04 Dec 2002 19:59:56 -0500
Subject: Re: Cleaning ATW Window on Oxford X-ray Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter et al,
I've been given 2 very good reasons not to use acetone (Freon TF or
isopropyl alcohol are preferred).

1) A long time ago I was told that the 7.5u Be window was actually
porous and the micro-pores were actually filled with oil - which acetone
very effectively removes, leaving a deteriorating vacuum.

2) More recently on this listserver it was noted that acetone is very
likely to attack whatever adhesive/bonding agent is used to fix the
window in place. Also a vacuum problem.

I would definitely ask Oxford and if it's a light element window... I
had one break while gently removing the collimator. Neither I nor my
customer were very pleased about that! I was going to clean it for him
with IPA but there was this terrible sucking sound...

High lN2 consumption is due to poor dewar vacuum. If the dewar is
tight, it'll be good for 20 years or more. If the consumption has risen
in a newer dewar, there is a leak that needs to be fixed first.

Good luck, you may need it.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA 17314

Peter Tomic wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Wed Dec 4 19:25:44 2002



From: James, Ed :      Ed.James-at-kla-tencor.com
Date: Wed, 4 Dec 2002 17:16:20 -0800
Subject: JEOL 733 uProbe: Smallest spot size?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to do some X-ray analysis on the JEOL 733 with an electron
probe size of 10nm-100nm.

Does anyone know whether this is feasible and what probe current I can
expect?
What is the smallest probe size and corresponding current that can be
achieved on the 733?
Also, I'd be interested to know the Cs, Cc and typical beam convergence
semi-angle for the standard objective lens at 15mm working distance.

Thanks,
Ed James.


From daemon Thu Dec 5 07:23:28 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 05 Dec 2002 08:06:06 -0500
Subject: Beryllium windows

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ken Converse wrote:
=============================================================
Peter et al,
I've been given 2 very good reasons not to use acetone (Freon TF or
isopropyl alcohol are preferred).

1) A long time ago I was told that the 7.5u Be window was actually
porous and the micro-pores were actually filled with oil - which acetone
very effectively removes, leaving a deteriorating vacuum.

2) More recently on this listserver it was noted that acetone is very
likely to attack whatever adhesive/bonding agent is used to fix the window
in place. Also a vacuum problem.

I would definitely ask Oxford and if it's a light element window... I had
one break while gently removing the collimator. Neither I nor my customer
were very pleased about that! I was going to clean it for him with IPA but
there was this terrible sucking sound...
============================================================
If beryllium foil was porous to the extent indicated by Ken, it would be
virtually impossible to make it vacuum tight (and these foils are made by
intention to be vacuum tight).

Secondly, beryllium windows and foil should be UHV clean when they leave the
manufacturer of the foil. Having the surface impregnated with oil as
described just does not sound right (or possible).

Attempting to clean an ultra-thin beryllium window unsupported would be an
extremely delicate operation. If not done properly, the window could be
broken, especially if it were under differential pressure at the time. The
best way would be to equalize the pressure, preferably at atmosphere if
possible, and then carefully provide a solid support for the window during
cleaning. This would reduce risk of damage, but not eliminate it. Once the
window is mounted, it is always going to be difficult to clean, especially,
to clean safely. Of course, this is not the kind of thing that could be
done in the typical SEM/EDS laboratory.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Thu Dec 5 07:57:00 2002



From: Frank Thomas :      thomasf-at-gsca.NRCan.gc.ca
Date: Thu, 5 Dec 2002 09:48:40 -0400
Subject: Varian Pump Control Unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers -

Under the "faint hope", "last chance" and "final straw" category, would
anybody out there have an old Varian VacIon Pump Control Unit Model 921-0062
(circa 1989) out there? Ours (which controls the ion pump on our ESEM) is in
dire need of a new reset relay. This is a complex little device, a
Potter-Brumfield 1537, which is no longer made. P-B says it's obsolete, and
was only made in small numbers under special order (to Varian, I assume).
Varian, helpfully, says "Gee, that part's obsolete, we don't have any, try
Potter-Brumfield".
If I can obtain one from a retired Varian unit, that would be a nice
easy fix. Failing that, our in-house electronics technologists/engineers
will have to bypass it some way, and replace it with an off-the shelf unit,
though this would need some modifications. And the instrument's down until
that happens.
I've had luck in the past obtaining equally arcane items from the folks
on this List, so I'm giving it a shot.

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia



From daemon Thu Dec 5 09:32:10 2002



From: Julia Kuerner :      kuerner-at-biochem.mpg.de
Date: Thu, 05 Dec 2002 16:20:59 +0100
Subject: phospholipase protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a protocol for lysis of cells (bacteria) using
phospholipase? And which phospholipase would be most suitable?

I would be grateful for any suggestions and thank everyone in advance!

Julia Kuerner



From daemon Thu Dec 5 09:37:59 2002



From: Paul.Nolan-at-alcan.com
Date: Thu, 5 Dec 2002 10:31:00 -0500
Subject: molds et al

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone direct me to an online source where i can try to identify molds
and fungi?
We had some green stuff growing on a large wood spool.
Under the SEM the stuff looks like bunches of grapes with each "grape"
about 2 microns.
Any of you biological types out there want to hesitate a guess? Mold
spores? fungus?
Any help would be appreciated.

Cheers

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



From daemon Thu Dec 5 09:50:16 2002



From: Anjeanette Ormonde :      Anjeanette.Ormonde-at-unilever.com
Date: Thu, 5 Dec 2002 08:46:00 -0600 (Central Standard Time)
Subject: kid's microscope suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know there has been some discussion about microscopes for kids lately
but I'd like to ask about a specific scope. A co-worker has a 6 year old
who has asked for a microscope. He is looking at the "Quantum Big Screen
Microscope" through the Learning Resources catalog but is concerned about
the quality of the microscope. Does anyone know anything about the
quality of this instrument or of the manufacturer? I'm assuming
everything is plastic - does that mean it will scratch easily or are the
"optics" protected? How good are the images it produces? Any comments on
this particular microscope or suggestions for a better one would be
greatly appreciated.

Angie




From daemon Thu Dec 5 10:06:30 2002



From: Michael L. Boucher :      mboucher-at-tc.umn.edu
Date: Thu, 05 Dec 2002 10:07:20 -0600
Subject: EM Technologist position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The University of Minnesota Characterization Facility has a new position
opening up for an EM person with some experience in TEM sample prep. See the
link below for more details on how to apply, etc. All applications must go
through our HR department. You may e-mail me, but unless you formally apply,
you cannot be considered.

http://www1.umn.edu/ohr/employ.html

The link for the position description (partially copied below) is:
http://www1.umn.edu/ohr/jobs/R117717.html

Requisition#: 117717
Job Title: Junior Scientist
Working Title: Electron Microscopy Technologist
Job Code Pay Range: $ 12.36 -$ 19.99 /Hour
Qualifications: A two-year associate degree in electron microscopy and 2
years experience in electron microscopy with experience in standard TEM
techniques including sample preparation, embedding, thin sectioning and
positive and negative staining. SELECTION CRITERIA/PREFERRED: Ranking
primarily based on total years of experience in electron microscopy.
Preference will be given to persons having an associate degree and
experience in biological or medical science related area transmission
electron microscopy. Bachelor's degree, experience in sample preparation
techniques, TEM and digital imaging will be taken into consideration in
ranking.
Duties: The Institute of Technology's Characterization Facility is seeking
candidates for a position as an electron microscopy technologist in an
active core research laboratory. The facility houses 4 TEMs and 6 SEMs, as
well as appropriate preparation equipment. In addition there is an
integrated suite of specimen characterization equipment maintained within
the facility. The position requires an individual experienced in standard
TEM techniques including samples preparation, embedding, thin-sectioning,
positive and negative staining. Experience in cryo-TEM and facility in
digital imaging would be a benefit. The applicant will be expected to
perform routine use and maintenance of the microscopes and preparation
equipment, as well as basic training of new users of the facility. 40% EM
operation and maintenance. 40% Sample Preparation. 10% Training. 10% Lab
Administrative duties.

********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************








From daemon Thu Dec 5 10:54:49 2002



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Thu, 5 Dec 2002 11:41:06 -0500
Subject: LM, Digital Image workshop Corrected

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What does that relay do? What's on the input, what's on the output? Is it
just a plain relay, or a latching relay, or a time delay relay? Or anything
else?


Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: Frank Thomas {thomasf-at-gsca.NRCan.gc.ca}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, December 05, 2002 8:48 AM


} I apologize! I accidently sent the first draft which had the wrong dates.
} The correct announcement is below.
}
} New York Microscopical Society
}
} 30 North Mountain Avenue
}
} Montclair, NJ 07042
}
}
}
}
}
}
}
}
} Bernard Friedman
}
}
} Memorial Workshop
}
}
}
}
}
}
} Digital Image Capture and Management in Microscopy
}
} May 8, 2003
}
}
} A course on Digital imaging in light microscopy which will cover the
} following topics:
}
}
} Optical Limitations in Light Microscopy...Photographic Imaging
Strategies...
} Digital Imaging Strategies...Selection of Digital Capture (Camera vs.
} Scanner)...
}
} Image Processing of Captured Images...Image File Formats...Printing
} Images... Color Management Systems...Database Management
} Software...Presentation Software for Oral Reports...Website
} Performance...Integration of Image Data with Sample Information,
} Calibration, Other Data & Reports...Acrobat and html Software for Written
} Reports and Archives...Examples of Efficient, Low Cost Image Handling
} Systems...
}
} Examples of Electronic Microscopy Reports and Databases
}
}
} The course instructors are Mary and John McCann of McCann Imaging.
}
} WHEN: Thursday, May 8, 2003, from 9 A.M. to 5 P.M.
}
} WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043
}
} COST: $350 for N.Y.M.S. members, $380 for non-members (includes
membership)
} Lunch and course materials are included. Checks made out to N.Y.M.S.
}
} HOW: Register using the form below. Limited to the first 12 registrants.
}
} Return form with a check to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ
} 07410.
}
} FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 e-mail
} donoleary-at-att.net
}
} PLEASE POST
}
} --------------------------------------------------------------------------
--
} ------------------------------------------------
}
} Digital Image Capture Registration Form
}
} N.Y.M.S. Member_________________ ($350) Non-Member__________($380)
}
} Name____________________________________________________________________
}
} Address__________________________________________________________________
}
} Phone (W)_____________________(H)_____________________Fax_________________
}
} e-mail________________________________________
}
}



From daemon Thu Dec 5 11:35:44 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 5 Dec 2002 11:27:26 -0600
Subject: TEM: Short filament life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I'm looking for some feedback/opinions/help on a recurring problem we're having with short filament life in our TEM. The last one got 64 hours....way, way less than experience says we should be getting.

Filament tip to Wehnelt distance is per manufacturer's specs (1 mm), set according to manufacturer's procedures. The gun is meticulously cleaned upon replacement, with all visible tungsten being removed with a combination of sonication in ammonia and polishing with metal polish. The gun components are then cleaned in ultrapure water, ethanol, and acetone, carefully dusted with compressed air, and reinstalled while wearing clean gloves. The anode chamber is dusted and O-ring inspected and cleaned, if necessary. Gun vacuum is good. We run the filament at 10 uA and it's saturating where the service engineers say it should be saturating at, in terms of bias settings and filled-in halos, etc.

The burnt out filaments exhibit normal narrowing , but the wire below the failure shows a small bulb, indicative of oversaturation. The puzzling part to me is that we're getting sheets (literally) of tungsten around the Wehnelt aperture, so thick that they are peeling away. Picture a dry mud-flat with patches of mud curling back on themselves away from the ground, only made of tungsten and surrounding an aperture! I have been told that this tungsten can be deposited in an instant as the filament dies a violent death, but I have never seen it on any other instrument, ever. This is happening whether I install the new filament or the service engineers install them. Oh, yeah, the filaments are OEM standard issue, not rebuilds, but in the past it has happened to rebuilds, too.

Any ideas? Thanks!

Sick of installing filaments,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Dec 5 12:13:17 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Thu, 5 Dec 2002 12:04:10 -0600
Subject: RE: Beryllium windows

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is it more difficult to clean Be window than UTW?

} Attempting to clean an ultra-thin beryllium window
} unsupported would be an
} extremely delicate operation. If not done properly, the
} window could be
} broken, especially if it were under differential pressure at
} the time. The
} best way would be to equalize the pressure, preferably at
} atmosphere if
} possible, and then carefully provide a solid support for the
} window during
} cleaning. This would reduce risk of damage, but not
} eliminate it. Once the
} window is mounted, it is always going to be difficult to
} clean, especially,
} to clean safely. Of course, this is not the kind of thing
} that could be
} done in the typical SEM/EDS laboratory.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.2spi.com
} ########################
} ============================================
}
}
}
}


From daemon Thu Dec 5 12:25:14 2002



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Thu, 05 Dec 2002 14:13:36 -0300 (ADT)
Subject: ptinters-Minolta

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi dear Lisetservers;
I have to buy a new printer for my EM lab (our old printer refuses to
work properly). Searching the web site, I came across a printer
Minolta-Magicolor 2200 DeskLaser with 1200 dpi and up to 96 Mb
RAM. I would like to use it for office printing as well as for working
quality electronmicrographs (I expect a lot form working quality).
Has any of you used this kind of printer? I would like to know how
reliable it is and what is the quality of printing. Can it be used for
printing electronmicrographs?
Thanks
Dorota


From daemon Thu Dec 5 12:30:56 2002



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Thu, 05 Dec 2002 14:20:17 -0300 (ADT)
Subject: knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello;
Our EM lab received a grant to buy a new knifemaker (for histo and
thin knives). I have a few questions to you:
1.Is Leica the only supplier of knifemakers in North America?
2.Are there any other supplier?
3.If yes, what is the quality of their equipment and how can I reach
them?
Please send your replies.
Thanks
Dorota



From daemon Thu Dec 5 15:00:31 2002



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Thu, 05 Dec 2002 14:47:20 -0600
Subject: Re: molds et al

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Paul,
Can you send me, or the microscopy listserve, the SEM picture of
your mold?
Illustrated Genera of Imperfect Fungi by Barnet & Hunter (APS
Press, 1998) might help.
Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242-1324

At 10:31 AM 12/5/2002 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Dec 5 15:32:00 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 06 Dec 2002 12:53:07 +1300
Subject: the whoosh of a disappearing vacuum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ed,

The spacial resolution with which you can do EPMA analysis is ultimately
limited not by the probe diameter but by the finite interaction volume which is a
result of the incident electrons' not giving up all of their energy at the point of
impact, but by multiple interactions within a teardrop-shaped volume below the
surface. The volume's diameter at the surface depends on the accelerating
voltage and on the specimen's mean atomic number, but is typically a couple
of microns.

A picture is better, there are several good articles on EPMA on the www.

One, by James Wittke of Northern Arizona University, is at
http:jan.ucc.nau.edu/~wittke/Microprobe/ProbeNotes, another is the set of
class notes for course 12.141 by Nilanjan Chatterjee, on the MIT website.

So your hoped-for resolution of 10nm to 100nm is not attainable, unless you
go to a thin-film sample.

cheers

rtch






} From: "James, Ed" {Ed.James-at-kla-tencor.com}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}


} I
} had one break while gently removing the collimator. Neither I nor my
} customer were very pleased about that! I was going to clean it for
} him with IPA but there was this terrible sucking sound...
}

Yes, it's a dreadful sound, much more expensive than the clicking of a dying ZIP disk.

I, too, have heard it.

In my case it was an expansive hand-waving gesture by the detector's owner, whose
finger-nail just clipped the window.

The detector was dead anyway, so we could almost laugh.

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Dec 5 18:25:37 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Thu, 05 Dec 2002 19:25:45 -0500
Subject: Re: TEM: Short filament life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dorta,
The Magicolor 2200 is a pretty good printer - fast, reliable, never a
paper jam. It gives good color rendition/reproduction, has a built in fast
Ethernet port. However(there is always one), the actual resolution is
600dpi hardware, 1200dpi interpolated. But, by using a good quality coated
inkjet or laser paper, digital images come out very well indeed. Find a
paper with a brightness of at least 95, it really makes a difference.
I sometimes bundle this printer with the ORION SEM Digital Imaging
System my company sells. My customers really like it, just for the reasons
you inquired about.
DISCLAIMER - I do not work for the Minolta Corporation, just a satisfied
user/reseller of their products.

Gary M. Easton, Pres.
Scanners Corporation
410-857-7633

----- Original Message -----
} From: "Dorota Wadowska" {wadowska-at-upei.ca}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, December 05, 2002 12:13 PM


Randy,
It sounds like you may have some instability in your filament drive
circuit. At some point the drive increases and you don't notice it
because you're already saturated. I just had a similar problem with a
customer's SEM. As near as I can tell it was just some oxidation on the
low voltage connector for the filament drive on the HV tank. After
exercising the connector, it's been OK for over a week. I've also seen
flaky Op Amps and transistors intermittently do this sort of thing,
also. It's going to require some long-term monitoring of the filament
drive circuit. If you're lucky, it'll be on the low voltage side.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Tindall, Randy D. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Fri Dec 6 05:06:42 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Fri, 6 Dec 2002 10:56:02 -0000
Subject: Old x-ray equipment, anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,
we are having yet another clear out of old cupboards with unused
equipment. If anyone is interested in having two Nuclear Enterprises
scintillation counters (Be window) model DM1-1 before they go for landfill
(with Be removed, of course), let me know asap. You will have to pay for
collection.

Richard


_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com



=======================================================================
This e-mail is intended for the person it is addressed to only. The
information contained in it may be confidential and/or protected by
law. If you are not the intended recipient of this message, you must
not make any use of this information, or copy or show it to any
person. Please contact us immediately to tell us that you have
received this e-mail, and return the original to us. Any use,
forwarding, printing or copying of this message is strictly prohibited.
No part of this message can be considered a request for goods or
services.
=======================================================================
Any questions about Bookham's E-Mail service should be directed to postmaster-at-bookham.com.



From daemon Fri Dec 6 08:09:21 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 06 Dec 2002 07:59:46 -0600
Subject: Re: knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dorota,

http://www.ebsciences.com/

They sell the GKM knifemaker, which breaks rectangles instead of
strips, and can make knives up to about 1/2" wide. I found this
knifemaker to be much better than any other.
Personal experience only, mind, and I have no ties to the company.

Phil

} Hello;
} Our EM lab received a grant to buy a new knifemaker (for histo and
} thin knives). I have a few questions to you:
} 1.Is Leica the only supplier of knifemakers in North America?
} 2.Are there any other supplier?
} 3.If yes, what is the quality of their equipment and how can I reach
} them?
} Please send your replies.
} Thanks
} Dorota

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Dec 6 08:09:23 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 6 Dec 2002 08:58:50 -0500
Subject: Re: Cleaning ATW Window on Oxford X-ray Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ken;

You are correct on the acetone. It was IPA. My apologies. It's my fading
memory that needs to be addressed.

I should add that the cleaning was done while the detector was still in the
collumnator with the nose piece still in place. The reason this was done in
the first place was that the detector was on an old Hitachi S570 with diff.
pump and someone tried to pump the column down while the exchange chamber
door was wide open! The entire column had to be cleaned including detector.

Multi-user facilities are a nightmare! I often suggest coin operated
analytical equipment to pay for the service contracts.

Peter

-----Original Message-----
} From: qualityimages [mailto:qualityimages-at-netrax.net]
Sent: Wednesday, December 04, 2002 8:00 PM
To: Peter Tomic; Microscopy




From daemon Fri Dec 6 08:53:43 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 6 Dec 2002 08:45:37 -0600
Subject: Re: TEM:Short Filament Life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wow! Thanks for all the replies that I've received on this topic. They're still coming in! Lots of very interesting possibilities, ranging from chronic oversaturation, to potential problems with ammonia cleaning, to power supply glitches.

If nobody objects, I would like to compile a summary of these responses for the list.

Also, I was asked why I didn't name the equipment that the problem was on. It's because on several occasions when I have done so, manufacturers and/or their service people have gotten quite excited about implied public criticism of their equipment, when I was just looking for general information and ideas. Except for the filament life problem, we are very happy with this scope, so I didn't see a real need to be more specific in this case. If anyone is curious, I'll be happy to identify the scope off-list.

Thanks again. You folks are great!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Fri Dec 6 09:03:53 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Fri, 6 Dec 2002 14:56:18 -0000
Subject: Spare books as well

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I also have several spare IoP series conference proceedings which are
available if anybody wants them.

Ser. No. 67 Microscopy of Semiconducting Materials 1983
Ser. No. 76 Microscopy of Semiconducting Materials 1985
Ser. No. 67 Microscopy of Semiconducting Materials 1983
Ser. No. 100 Microscopy of Semiconducting Materials 1989
Ser. No. 117 Microscopy of Semiconducting Materials 1991
Ser. No. 134 Microscopy of Semiconducting Materials 1993
Ser. No. 146 Microscopy of Semiconducting Materials 1995

Ser. No. 68 Electron Microscopy and Analysis 1983
Ser. No. 90 Electron Microscopy and Analysis 1987
Ser. No. 93 Volumes 1 and 2 EUREM 1998

As before, first come, first served, & your collection method has to be as
cheap as me putting them in the bin!

Richard
_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com


=======================================================================
This e-mail is intended for the person it is addressed to only. The
information contained in it may be confidential and/or protected by
law. If you are not the intended recipient of this message, you must
not make any use of this information, or copy or show it to any
person. Please contact us immediately to tell us that you have
received this e-mail, and return the original to us. Any use,
forwarding, printing or copying of this message is strictly prohibited.
No part of this message can be considered a request for goods or
services.
=======================================================================
Any questions about Bookham's E-Mail service should be directed to postmaster-at-bookham.com.



From daemon Fri Dec 6 09:45:24 2002



From: Andrew Werner :      werner-at-rosharon.oilfield.slb.com
Date: Fri, 06 Dec 2002 09:13:35 -0600
Subject: Re: Varian Pump Control Unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frank,

Sorry, don't have one - but I get parts for our Varian ion pump from an
outfit called Duniway stockroom. At
http://www.duniway.com/html/ion_contro-surplus.htm they list a rebuilt
921-062 control unit for $1250. Maybe they can rebuild yours or do some
kind of swap. Hope this helps. Their number is 1-800-446-8811.

Regards,
Andrew

Disclaimer: I don't work for Duniway of have any financial interest in the
company; just a satisfied customer.



At 09:48 AM 12/5/2002 -0400, Frank Thomas wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Dec 6 11:30:25 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 6 Dec 2002 09:15:29 -0800
Subject: Re: TEM: Short filament life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Thursday, December 5, 2002, at 09:27 AM, Tindall, Randy D. wrote:

} I'm looking for some feedback/opinions/help on a recurring problem
} we're having with short filament life in our TEM. The last one got 64
} hours....way, way less than experience says we should be getting.
}
} We run the filament at 10 uA and it's saturating where the service
} engineers say it should be saturating at, in terms of bias settings
} and filled-in halos, etc.
}
} The burnt out filaments exhibit normal narrowing , but the wire below
} the failure shows a small bulb, indicative of oversaturation.

} Any ideas? Thanks!
}
Dear Randy,
I have two ideas: 1) Filament lifetime is longer if the current is
just at the saturation point; is 10 uA higher than this? If there is
evidence of oversaturation, the current may be too high. 2) Did you
heat the filaments in, say, a vacuum evaporator before you installed
them? Sometimes the first heating will cause the filament to warp due
to stresses built up during manufacture; this will cause the position
of the filament to change if the warping occurs in the Wehnelt and may
contribute to shorter life and lower beam current. Are all the
filaments from the same batch? Good luck.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Fri Dec 6 13:10:32 2002



From: John R Reffner :      rsrj2r-at-rohmhaas.com
Date: Fri, 6 Dec 2002 15:56:02 -0500
Subject: RE: Image Handling Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I got my neurologist a used single power stereo microscope that his 5 year
old loves. Some think that monocular work better for young kids because
there is less to adjust. When you find them monocular low powered scope that
have inverting prisms sell for a song.

http://www.couger.com/microscope/links/gcnewbuy.html Is a page that I keep
that cover a lot of issues on buying used microscopes form several peoples
points of veiw. I am convinced you get a great deal more for your money
buying a good used brand name scope if you can find one that fits you needs
and doesn't have too many gadgets for the kids. I have sitting on my bench
right now a good recent imported Asian Scope, a classic B&L Stereo Zoom 7
and a old AO Cycloptic. They are all satisfactory scopes but I reach for the
old AO because of the brighter image than the rest. I am cleaning the Asian
Scope and while difficult to align it is not much more so than any other.
The prisms aren't coated with aniti reflective coatings. The images aren't
as bright because the telescopes are smaller and there is plastic used in
many places.

I have no commercial interests is selling microscopes but I will always do
everything I can to help someone get a kid started in science so anything I
can do to help please fell free to ask.


Gordon
Gordon Couger gcouger-at-couger.com

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward any links or information you think might be useful to others.

----- Original Message -----
} From: "Anjeanette Ormonde" {Anjeanette.Ormonde-at-unilever.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, December 05, 2002 8:46 AM


Thumbs Plus from Cerious Software.

Similar to ACDC, but I happen to like it better. We use it for

- Image file management
- Contact Sheet Printing
- Batch image processing
- Web pages with thumbnails

We use it on several different computers.



http://www.cerious.com/



----------------------------------------------------------------------------
--------------------------------
John Reffner, ACTC Microscopy Group Leader, Rohm and Haas Co.
215-619-5283
JReffner-at-rohmhaas.com
------------------- Opinions expressed are mine and -----------------------
------------------- not those of Rohm and Haas
Company -----------------------




From daemon Fri Dec 6 16:05:13 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Fri, 6 Dec 2002 16:54:22 -0500
Subject: knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1) No.

2) Energy Beam Sciences manufactures both Triangular and Ralph (Long) Glass
Knifemakers. RMC Boeckeler also makes a Triangular Knifemaker. Those are
the only others I am aware of.

3) EBS has been manufacturing these Knifemakers for many years and has many
satisfied Customers around the world. You can reach us at
ebs-at-ebsciences.com or by phone on (413) 786-9322.

Sincerely,
Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
11 Bowles Road
Agawam, MA 01001-2925
Tel: (413) 786-9322
Fax: (413) 798-2786
"Adding Brilliance to Your Vision"

Disclaimer: Writer is an employee of and has a financial interest in Energy
Beam Sciences, Inc.




-----Original Message-----
} From: Dorota Wadowska [mailto:wadowska-at-upei.ca]
Sent: Thursday, December 05, 2002 12:20 PM
To: microscopy-at-sparc5.microscopy.com


Hello;
Our EM lab received a grant to buy a new knifemaker (for histo and
thin knives). I have a few questions to you:
1.Is Leica the only supplier of knifemakers in North America?
2.Are there any other supplier?
3.If yes, what is the quality of their equipment and how can I reach
them?
Please send your replies.
Thanks
Dorota





From daemon Fri Dec 6 16:28:10 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 6 Dec 2002 17:19:32 -0500
Subject: knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Afternoon Darota:
I bought a JB-4 Knifemaker years ago when the JB-4 was first
produced by Dupont. That entire system is now manufactured and sold by
Energy Beam Sciences.

http://www.ebsciences.com/microtome/knifemaker.htm

They have both a triangular knife maker and a Ralph knife maker. I only
have experience with the original triangular knife maker and was happy with
it.

I have no relationship to EBS other than as a customer of Dupont years ago.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone: 610-738-0437
eMail: fmonson-at-wcupa.edu


-----Original Message-----
} From: Dorota Wadowska [mailto:wadowska-at-upei.ca]
Sent: Thursday, December 05, 2002 12:20 PM
To: microscopy-at-sparc5.microscopy.com


Hello;
Our EM lab received a grant to buy a new knifemaker (for histo and
thin knives). I have a few questions to you:
1.Is Leica the only supplier of knifemakers in North America?
2.Are there any other supplier?
3.If yes, what is the quality of their equipment and how can I reach
them?
Please send your replies.
Thanks
Dorota



From daemon Fri Dec 6 16:35:53 2002



From: MicroscopyToday :      microtod-at-optonline.net
Date: Fri, 06 Dec 2002 17:25:55 -0500
Subject: Re: TEM:Short Filament Life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good for you, Randy! I cringe every time I see someone posting a
doubtful criticism of a named manufacturer on the public listserver
when, oftentimes, the problem is due to the poster's poor technique or
failure to understand the basics of microscopy.

Who can forget the criticism of a TEM manufacturer's so-called unstable
specimen stage when it turned out that the complainer did all of his
examination, focusing, and plate exposure of biological thin sections at
crossover with the brightest C1 setting because "It's easier to work
with a bright image."

Ron Anderson

-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Friday, December 06, 2002 9:46 AM
To: microscopy-at-sparc5.microscopy.com


Wow! Thanks for all the replies that I've received on this topic.
They're still coming in! Lots of very interesting possibilities,
ranging from chronic oversaturation, to potential problems with ammonia
cleaning, to power supply glitches.

If nobody objects, I would like to compile a summary of these responses
for the list.

Also, I was asked why I didn't name the equipment that the problem was
on. It's because on several occasions when I have done so,
manufacturers and/or their service people have gotten quite excited
about implied public criticism of their equipment, when I was just
looking for general information and ideas. Except for the filament life
problem, we are very happy with this scope, so I didn't see a real need
to be more specific in this case. If anyone is curious, I'll be happy
to identify the scope off-list.

Thanks again. You folks are great!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/







From daemon Fri Dec 6 16:41:31 2002



From: MicroscopyToday :      microtod-at-optonline.net
Date: Fri, 06 Dec 2002 17:26:23 -0500
Subject: Re: TEM:Short Filament Life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good for you, Randy! I cringe every time I see someone posting a
doubtful criticism of a named manufacturer on the public listserver
when, oftentimes, the problem is due to the poster's poor technique or
failure to understand the basics of microscopy.

Who can forget the criticism of a TEM manufacturer's so-called unstable
specimen stage when it turned out that the complainer did all of his
examination, focusing, and plate exposure of biological thin sections at
crossover with the brightest C1 setting because "It's easier to work
with a bright image."

Ron Anderson

-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Friday, December 06, 2002 9:46 AM
To: microscopy-at-sparc5.microscopy.com


Wow! Thanks for all the replies that I've received on this topic.
They're still coming in! Lots of very interesting possibilities,
ranging from chronic oversaturation, to potential problems with ammonia
cleaning, to power supply glitches.

If nobody objects, I would like to compile a summary of these responses
for the list.

Also, I was asked why I didn't name the equipment that the problem was
on. It's because on several occasions when I have done so,
manufacturers and/or their service people have gotten quite excited
about implied public criticism of their equipment, when I was just
looking for general information and ideas. Except for the filament life
problem, we are very happy with this scope, so I didn't see a real need
to be more specific in this case. If anyone is curious, I'll be happy
to identify the scope off-list.

Thanks again. You folks are great!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/







From daemon Fri Dec 6 16:44:47 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 06 Dec 2002 17:18:54 -0800
Subject: Re: knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Randy,
It sounds like you are doing all the right things, except checking the
saturation level after the filament has run for a while. I re-check the
saturation level every running hour for at least five hours and then once a
day after that, because the saturation point actually decreases slightly as
the filament ages. This is a curve, with the steepest drop in saturation
temp at the beginning. The other thing I do is run with the slightest dark
spot remaining in the condenser crossover image. This is a quick way to
check that you are still slightly undersaturated.
Good luck.
Mary
----- Original Message -----
} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, December 05, 2002 9:27 AM


Phil, I do agree that GKM knifemaker is good. Unfortunately I have
extremely bad experience with EM Sciences. When I am shopping for
equipment, my rule is that I have to see how equipment works before I buy
it (demo or exibition). Like car, you have to try it first. I was asking
EM Sciences for the demo. They refused. Finally after discussion on the
high level they did agree to sent to me instrument for demo and I'll buy it
if I like it... I DID sign a special 5 pages Contract (never did before),
they supposed to sent instrument after that. Now it's about a year as they
DO promise to send the instrument. When I called them a month later after
signing the Contract - it was a panic on the other end - they were urgently
assembling the instrument (they did not have any at hand). So, they were
lay when sign the Contract (there were exact dates on the Contract for
shipping). I told them, OK, I'll wait. So, I am waiting and waiting...
until now. I do have all paperwork from that case to verify that the story
is true. It looks to me that Leica is only a possibility for majority of
us. I really don't like old RMC knifemaker, but newer try the current
model. Personally I did not try 10 mm on Leica, but 8 mm knifes were really
good, no complains. I don't have Leica instrument, I sort of wait for
response from EB Sciences... meantime using 10 mm W-carbide knifes from
DDK. By the way, EB Sciences was trying to sell to me Leica instead GKM,
so they definitely have some problems with GKM. I think this is a purpose
of our ListServer: to share experience, bad and good. I wish everyone a
great Holiday Season and according Russian tradition, I wish, all your
wishes (at least a few of them) become true in the coming New Year. Sergey

At 05:59 AM 12/6/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Sat Dec 7 10:20:02 2002



From: amostaghimi-at-onyx-pharm.com (by way of MicroscopyListserver)
Date: Sat, 7 Dec 2002 10:10:47 -0600
Subject: Ask-A-Microscopist: images of human adenovirus particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (amostaghimi-at-onyx-pharm.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
December 6, 2002 at 19:38:16
---------------------------------------------------------------------------

Email: amostaghimi-at-onyx-pharm.com
Name: ALI MOSTAGHIMI

Organization: ONYX PHARMACEUTICALS

Education: Graduate College

Location: RICHMOND, CA 94806

Question: I am interested to find out about EM technology that can
show images of human adenovirus particles that my have extraneous
pieces of DNA stock to them. Do you think it is possible to produce
images with these two requirements? Your reply is appreciated.


Best Regards,

Ali Mostaghimi
QC manager, project services
Onyx Pharmaceuticals
3031 Research Drive
Richmond, CA 94806
Phone: 510-243-3697

---------------------------------------------------------------------------


From daemon Sat Dec 7 10:30:50 2002



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Sat, 7 Dec 2002 11:22:55 -0500
Subject: LM, Polarized Light Microscopy Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


New York Microscopical Society

30 North Mountain Avenue

Montclair, NJ 07042



Bernard Friedman

Memorial Workshop





Polarized Light Microscopy

April 26, May 3, 10 & 17, 2003


An advanced course on polarized light microscopy which will cover the
following topics:

The nature of polarized light

The origin and interpretation of interference colors

Birefringence and crystal orientation

The Indicatrix

Compensation and variable compensators

Interference figures and their interpretation


The workshop will consist of four Consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of polarized
light microscopy. The course instructors include Jan Hinsch of Leica, Inc.,
Mary McCann of McCann Imaging, John Reffner of SensIR and N.Y.M.S.
Instructor Don O'Leary.

WHEN: April 26, May 3, 10 &17, 2003, from 10 A.M. to 4 P.M.

WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043

COST: $340 for N.Y.M.S. members, $370 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: advanced course for those who have completed "The Use of the
Microscope" or are experienced in microscopy and familiar with the theory of
its use.

HOW: Register using the form below. Limited to the first 12 registrants.

Return form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 e-mail
donoleary-at-att.net

PLEASE POST

----------------------------------------------------------------------------
-------------------------------------

Registration Form

Polarized Light Microscopy

N.Y.M.S. Member_________________ ($340) Non-Member__________($370)

Name_____________________________________________________________

Address___________________________________________________________

Phone (W)_________________________(H)______________________________

e-mail________________________________________



From daemon Sat Dec 7 15:18:47 2002



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 7 Dec 2002 12:55:45 -0800
Subject: Re: kid's microscope suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I got my neurologist a used single power stereo microscope that his 5 year
} old loves. Some think that monocular work better for young kids because
} there is less to adjust. When you find them monocular low powered scope that
} have inverting prisms sell for a song.

You've got it slightly wrong, Gordon. One problem with a binocular
"dissecting" scope for young children is that the minimum interocular
spacing usually is too wide for their eyes. A second is that almost a
fifth of young kids have some problem with stereo vision that makes use of
a binoc frustrating. A third problem is that they occasionally drop
things, which throws out the ocular alignment. A fourth is that they cost
a lot more. 20x monocs are wonderful beginner scopes for children, and
good ones are available for $70 or less. Where? Look at the MICRO website
(URL below) and/or Email me.
}
Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun Dec 8 00:03:51 2002



From: Elliot Kyung-Ho Lee :      khlee-at-ybust.edu.cn
Date: Sun, 8 Dec 2002 13:50:20 +0800
Subject: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear members,

I am trying to build up an image analysis system with a metallographic microscope. I have a few microscopes except a CCD camera. Would you please recommend ccd cameras with low ~ medium price range. Thank you very much in advance.

Elliot Lee

*********************************************************
Professor Elliot Kyung-Ho Lee
Yanbian University of Science & Technology
School of Materials, Mechanical & Automation Engineering
Beishan Street, Yanji City, Jilin Province, 133000, CHINA
中國 å‰æž—çœ å»¶å‰å¸‚ 北山街 延邊科學技術大學 (郵)133000
æ料機械自動化工學部 敎授 李å¿è±ª
Office) +86-433-291-2978
Fax) +86-433-291-2510
*********************************************************



From daemon Sun Dec 8 08:11:15 2002



From: DrJohnRuss-at-aol.com
Date: Sun, 08 Dec 2002 09:00:16 -0500
Subject: Re: composite 2-D images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 12/4/2002 8:00:47 PM Eastern Standard Time, ramirez-at-amnh.org writes:

} Does anyone knows of a free or accessible software to compose in-focus 2-D
} images from several images in different focal planes?
} Martin

Not aware of any free, but the image processing tool kit does include that capability (plus many others) and is relatively inexpensive. see http://reindeergraphics.com




From daemon Sun Dec 8 10:22:07 2002



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sun, 8 Dec 2002 16:00:12 -0000
Subject: Re Filament Life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers

I know I am a bit late but have you seen this suggestion?

Randy what you are describing is exactly the symptom of poor quality
filaments, I
even have pictures to demonstrate what you describe within our maintenance
CD course.

} From time to time the wire used to manufacture filaments is not up to
standard, it contains a level of garbage; no criticism of the manufacturer
of the filaments more a criticism
of the wire maker! In these circumstances the filaments thin normally but a
good deal quicker and with far more of the evaporated "materials". Never
panic
with small blobs on the filament break as if an operator is unlucky this can
happen to the most experienced of us.

As you know I have worked with several electron microscope manufacturers and
every few years or
so this type of problem crops up.

My advice to EM operators is NEVER use a complete box of filaments that have
served you well; always save two. Then in the situation that you now have
you
can pop in one of a good box to prove you have a problem, or that the new
box
of filaments is of a poor quality.

So how should you proceed -
1. You need to run another filament from the new box with great care,
keep an eye on the operators to make sure you do not have an oversaturation
problem with one of them.
2. If the problem is repeated replace with a filament from a good box.
3. If the problem continues you probably have a leak in the gun area,
forget what the gauges say unless they are actually in the gun area.
4. If the problem went away you have proven the new filaments to be at
error, return them to the supplier with an explanation of your problem

Other areas of investigation if this does not solve your problem are-

1. The colour of the ceramic - yellow/brown suggests a vacuum leak
2. A smelly gun chamber - smells of oil/ozone - suggests a vacuum leak
3. Normal filament break but THE WHOLE OF THE FILAMENT HAS THINNED, not
just the tip - gas attack - suggests a vacuum leak

Hope this helps, maybe we could privately have a chat about saturation?

Regards

Steve Chapman
Senior Consultant Protrain
EM Training and Consultancy World Wide
Tel +44 (0)1280 816512 Fax +44 (0)1280 814007
www.emcourses.com






From daemon Sun Dec 8 14:16:57 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 09 Dec 2002 09:04:21 +1300
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I am trying to build up an image analysis system with a metallographic
} microscope. I have a few microscopes except a CCD camera. Would you
} please recommend ccd cameras with low ~ medium price range. Thank you
} very much in advance.

I've had quite a lot of success interfacing cheap (US$200) CCTV cameras to
microscopes. If the eyepiece is detachable from the scope, you can just offer
up the camera without lens. If you can't remove the eyepiece, you need to use
a lens on the camera, focussed to infinity, and as close as possible to the
eyepiece.

You can feed the composite video signal from the CCD camera to a TV (AV
input), or to a video capture card (about US$100) in a computer. That way you
can capture, store, and print the images.

Quality is OK for enlargement up to about postcard size.

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sun Dec 8 14:34:39 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Sun, 08 Dec 2002 14:23:24 -0600
Subject: Re: knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey,

Bummer. I've never dealt with Energy Beam Sciences (not "EM sciences"
-- a typo?). Just the GKM knifemaker, and to boot, this was before
the knifemaker's name was changed to "GKM". The ones I knew were
Sorvalls. Another company bought the product, I guess, and now a good
product is down the tubes?

I completely agree about testing equipment before buying. More
importantly, test the company selling the product.

Phil

} Phil, I do agree that GKM knifemaker is good. Unfortunately I have
} extremely bad experience with EM Sciences. When I am shopping for
} equipment, my rule is that I have to see how equipment works before
} I buy it (demo or exibition). Like car, you have to try it first. I
} was asking EM Sciences for the demo. They refused. Finally after
} discussion on the high level they did agree to sent to me instrument
} for demo and I'll buy it if I like it... I DID sign a special 5
} pages Contract (never did before), they supposed to sent instrument
} after that. Now it's about a year as they DO promise to send the
} instrument. When I called them a month later after signing the
} Contract - it was a panic on the other end - they were urgently
} assembling the instrument (they did not have any at hand). So, they
} were lay when sign the Contract (there were exact dates on the
} Contract for shipping). I told them, OK, I'll wait. So, I am
} waiting and waiting... until now. I do have all paperwork from that
} case to verify that the story is true. It looks to me that Leica is
} only a possibility for majority of us. I really don't like old RMC
} knifemaker, but newer try the current model. Personally I did not
} try 10 mm on Leica, but 8 mm knifes were really good, no complains.
} I don't have Leica instrument, I sort of wait for response from EB
} Sciences... meantime using 10 mm W-carbide knifes from DDK. By the
} way, EB Sciences was trying to sell to me Leica instead GKM, so they
} definitely have some problems with GKM. I think this is a purpose of
} our ListServer: to share experience, bad and good. I wish everyone
} a great Holiday Season and according Russian tradition, I wish, all
} your wishes (at least a few of them) become true in the coming New
} Year. Sergey
}
} At 05:59 AM 12/6/2002, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Dec 8 14:39:09 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sun, 8 Dec 2002 13:24:49 -0700
Subject: image handling software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello Judy,

I think, some of the features you listed are available from Win2000 or WinXP
(if you are using a PC, that is). You can set your folders to preview images
as thumbnails (XP does a number of formats), and you can certainly copy and
move them around. XP also has a "filmstrip" mode, where you see one image
large and the others as smaller thumbnails along the bottom.

However, I think you are more looking for an image archive that you can
search and where you can provide additional information that you can use
later for finding images. We have that as part of our analySIS software, but
there are other applications out there as well. A solution like this usually
requires a bit more up-front work (you have to define the keywords and key
fields), but it will be much more efficient in the long run. If you check
for solutions like this, make sure you don't have to pay too much for
"reader" software (ours is free), and perhaps options for internet access to
the archive. Finally, although you are in Canada and this may only apply to
US companies, make sure that your archive or data base software can be made
rule 11 compliant if you have to deal with the FDA.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca]
Sent: Tuesday, December 03, 2002 11:32 AM
To: Microscopy-at-sparc5.microscopy.com


Fellow microscopists :

I recently collected a lot of images at a single sitting, each image
seemingly better than the previous one, definitely on a roll. However, the
next day I laboriously had to review a lot of images of similar data and
choose the best ones.

What software are people using to browse through a lot of images, as
thumbnails, easily change their names, perhaps add annotations, move
selected ones into new folders and create a catalogue. It should also be
able to recognize and load confocal image stacks.

Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Queen
30 Bond St.
Toronto, ON M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
pager: 416-685-9219
trogadisj-at-smh.toronto.on.ca




From daemon Sun Dec 8 15:13:10 2002



From: wuff-at-swisswuff.ch (by way of MicroscopyListserver)
Date: Mon, 9 Dec 2002 08:50:41 -0600
Subject: Re: composite 2-D images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Elliot,

Probably the lowest price solution would be Kodak MDS 100 (I bought one for
around $100 a while ago). However, it doesn't work with all the microscopes
and you might have to buy a video relay lens (adapter) for the camera, which
would cost you up to $200, at least. However, the camera I bought didn't
"impress" me with the image quality, particularly color wise. Therefore,
sometimes I use a color CCD camera (1/3"), a video lens and a video capture
device (it may be a computer card). The quality is just perfect and the
price is about the same, since it's possible to buy a relatively good CCD
camera for around $100.

Vladimir Igoshev, Ph.D.

Toronto, Canada

----- Original Message -----
} From: "Elliot Kyung-Ho Lee" {khlee-at-ybust.edu.cn}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, December 08, 2002 12:50 AM


I wrote some IDL code (http://www.rsinc.com) that does that. It is
just code, and there is no graphical user interface (yet). I create
an array which classifies the pixel of each image with a score, and
then extract the 'best scoring pixel' from each of the input images
in order to create an output image. The way that score is obtained
must relate to your 'feature size'; technically, I use the difference
of the blurred image (or spot area) to the original image for each
one of the images in order to calculate that score.

Example image:
http://www.swisswuff.ch/pnphoenix721/html/modules.php?op=modload&name=News&file=article&sid=5&mode=thread&order=0&thold=0


I am not sure if that is what you are after.

Wolf Schweitzer



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} In a message dated 12/4/2002 8:00:47 PM Eastern Standard Time,
} ramirez-at-amnh.org writes:
}
} } Does anyone knows of a free or accessible software to compose in-focus 2-D
} } images from several images in different focal planes?
} } Martin
}
} Not aware of any free, but the image processing tool kit does
} include that capability (plus many others) and is relatively
} inexpensive. see http://reindeergraphics.com


From daemon Mon Dec 9 09:14:27 2002



From: Berry :      Lorraine.Berry-at-naturalsciences.be
Date: Mon, 09 Dec 2002 16:07:31 +0100
Subject: Alternative sectioning solutions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi there

I am trying to locate trace metals in tissues. I have been cutting dry
sections since I cannot use water. Sectioning on water could move or
dissolve the trace metals I am after.

Could you recommend any references or techniques about alternatives to
using water for sectioning ?

Thanks for any help

L. Berry

.....................................................
Lorraine Berry
RBINS
Department of Invertebrates
Rue Vautier 29
Bruxelles
Lorraine.berry-at-naturalsciences.be
.....................................................



From daemon Mon Dec 9 10:42:27 2002



From: jan factor :      jfactor-at-purvid.ns.purchase.edu
Date: Mon, 09 Dec 2002 11:41:47 -0500
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've heard that there is a microscope adaptor for the Nikon CoolPix line of
cameras. Some of the CoolPix model are upper-end consumer cameras, which run up
to about $1000. Sorry, I don't know the details on the adaptor, but try an
internet search.
---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Division of Natural Sciences
Purchase College
State University of New York
Purchase, NY 10577
---------------------------------------
Ofc. Tel: 914-251-6659
Ofc. Fax: 914-251-6635
E-mail: jfactor-at-purvid.ns.purchase.edu
---------------------------------------


Ritchie Sims wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } I am trying to build up an image analysis system with a metallographic
} } microscope. I have a few microscopes except a CCD camera. Would you
} } please recommend ccd cameras with low ~ medium price range. Thank you
} } very much in advance.
}
} I've had quite a lot of success interfacing cheap (US$200) CCTV cameras to
} microscopes. If the eyepiece is detachable from the scope, you can just offer
} up the camera without lens. If you can't remove the eyepiece, you need to use
} a lens on the camera, focussed to infinity, and as close as possible to the
} eyepiece.
}
} You can feed the composite video signal from the CCD camera to a TV (AV
} input), or to a video capture card (about US$100) in a computer. That way you
} can capture, store, and print the images.
}
} Quality is OK for enlargement up to about postcard size.
}
} cheers
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand

--




From daemon Mon Dec 9 11:44:20 2002



From: roxie hartman :      janine.knox-at-access123.net
Date: Mon, 09 Dec 2002 12:33:57 -1700
Subject: Next generation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Mon Dec 9 12:41:48 2002



From: Martin Ramirez :      ramirez-at-amnh.org
Date: Mon, 09 Dec 2002 13:44:10 -0500
Subject: RE: composite 2-D images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking into getting digital camera and came across Nikon Coolpix
microscope kit

http://www.ipsimaging.com/products/cameras/coolpixkit.htm
A little bit pricey!

Regards,
Pavel


----- Original Message -----
} From: "jan factor" {jfactor-at-purvid.ns.purchase.edu}
To: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
Cc: "Elliot Kyung-Ho Lee" {khlee-at-ybust.edu.cn} ; {}
Sent: Monday, December 09, 2002 11:41 AM


Thanks to all who answered my question. There is a free program in
http://www.hadleyweb.pwp.blueyonder.co.uk/CombineZ/3/CZ3.htm
martin

} Does anyone knows of a free or accessible software to compose in-focus 2-D
} images from several images in different focal planes?
} Martin



From daemon Mon Dec 9 16:03:54 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 09 Dec 2002 13:54:42 -0800
Subject: Re: knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, it was typo, the company's correct name is Energy Beam
Sciences. Sorry. Sergey

At 02:23 PM 12/8/02 -0600, you wrote:
} Sergey,
}
} Bummer. I've never dealt with Energy Beam Sciences (not "EM sciences" -- a
} typo?). Just the GKM knifemaker, and to boot, this was before the
} knifemaker's name was changed to "GKM". The ones I knew were Sorvalls.
} Another company bought the product, I guess, and now a good product is
} down the tubes?
}
} I completely agree about testing equipment before buying. More
} importantly, test the company selling the product.
}
} Phil
}
} } Phil, I do agree that GKM knifemaker is good. Unfortunately I have
} } extremely bad experience with EM Sciences. When I am shopping for
} } equipment, my rule is that I have to see how equipment works before I buy
} } it (demo or exibition). Like car, you have to try it first. I was asking
} } EM Sciences for the demo. They refused. Finally after discussion on the
} } high level they did agree to sent to me instrument for demo and I'll buy
} } it if I like it... I DID sign a special 5 pages Contract (never did
} } before), they supposed to sent instrument after that. Now it's about a
} } year as they DO promise to send the instrument. When I called them a
} } month later after signing the Contract - it was a panic on the other end
} } - they were urgently assembling the instrument (they did not have any at
} } hand). So, they were lay when sign the Contract (there were exact dates
} } on the Contract for shipping). I told them, OK, I'll wait. So, I am
} } waiting and waiting... until now. I do have all paperwork from that case
} } to verify that the story is true. It looks to me that Leica is only a
} } possibility for majority of us. I really don't like old RMC knifemaker,
} } but newer try the current model. Personally I did not try 10 mm on Leica,
} } but 8 mm knifes were really good, no complains. I don't have Leica
} } instrument, I sort of wait for response from EB Sciences... meantime
} } using 10 mm W-carbide knifes from DDK. By the way, EB Sciences was
} } trying to sell to me Leica instead GKM, so they definitely have some
} } problems with GKM. I think this is a purpose of our ListServer: to share
} } experience, bad and good. I wish everyone a great Holiday Season and
} } according Russian tradition, I wish, all your wishes (at least a few of
} } them) become true in the coming New Year. Sergey
} }
} } At 05:59 AM 12/6/2002, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Dec 9 17:06:15 2002



From: Jim Haley :      haley-at-mvia.com
Date: Mon, 09 Dec 2002 17:57:08 -0500
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jan/Pavel,

We have Coolpix adapters available for most microscopes for mounting in
lieu of the eyepieces or through a trinocular port. Price range is from
$300 - $500 depending on the clamps needed for your particular setup.

Some advantages of our Nikon Coolpix adapters:

******************Better Quality*********************************

1.) One nice thing about our Coolpix system is that the optics system
is universal as far as the microscope is concerned. We use the same
optics no matter which scope you are using - you would just need a
different clamp to mount on various models of microscopes.

2.) If you would rather mount through the eyepieces of the scope, our
adapters will drop INTO the eyepiece receivers on the microscope and NOT
grip around them as some of the digital camera adapters on the market.
This is a much more stable way of doing things so you don't wind up with
a $700 camera falling off your scope.

3.) Our adapters also have correct optics and magnification for the
Coolpix series cameras built in so that you get the full zoom range of
the camera with no vignetting. There is neither chromatic aberration
or spherical aberration with our adapters. AND we stand behind them
with a 30 day money back guarantee.

*******************Easier to use************************************

4.) You don't have to screw it onto the microscopes - just leave the
adapter attached to your camera and all you'll have to do is pull the
eyepiece on your scope and drop the camera/adapter assembly in - rather
than trying to get a bunch of screws tightened on the eyepieces. If you
are going through the eyepieces, this makes it a lot easier to take the
camera off and on the microscope.

5.) It's less bulky and much cleaner than competing adapters so it
won't get in the way if you want to look in the other eyepiece while the
camera is attached to the microscope.

6.) You don't have to worry about getting the camera the correct
distance from the ocular to have the camera parfocal with the microscope
because our adapter slides into the eyepiece tube rather than clamping
around the eyepiece and having the camera at the incorrect optical
distance from the eyepiece.

7.) You don't have to worry about the magnification of the eyepieces in
your microscopes - our adapter has the correct magnification for the
Coolpix series cameras ALL the time.

Please feel free to call me or email me if you would like to discuss
your application in greater detail.

Thanks & Happy Holidays!
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
125 Sherwood Drive
Monaca, PA 15061
voice: (724) 728-7493
fax: (412) 291-1709
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************


atcsem wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am looking into getting digital camera and came across Nikon Coolpix
} microscope kit
}
} http://www.ipsimaging.com/products/cameras/coolpixkit.htm
} A little bit pricey!
}
} Regards,
} Pavel
}
} ----- Original Message -----
} } From: "jan factor" {jfactor-at-purvid.ns.purchase.edu}
} To: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
} Cc: "Elliot Kyung-Ho Lee" {khlee-at-ybust.edu.cn} ; {}
} Sent: Monday, December 09, 2002 11:41 AM
} Subject: Re: LM: CCD camera for LM
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I've heard that there is a microscope adaptor for the Nikon CoolPix line
} of
} } cameras. Some of the CoolPix model are upper-end consumer cameras, which
} run up
} } to about $1000. Sorry, I don't know the details on the adaptor, but try an
} } internet search.
} } ---------------------------------------
} } Jan Robert Factor, Ph.D.
} } Professor of Biology
} } ---------------------------------------
} } Division of Natural Sciences
} } Purchase College
} } State University of New York
} } Purchase, NY 10577
} } ---------------------------------------
} } Ofc. Tel: 914-251-6659
} } Ofc. Fax: 914-251-6635
} } E-mail: jfactor-at-purvid.ns.purchase.edu
} } ---------------------------------------
} }
} }
} } Ritchie Sims wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } } I am trying to build up an image analysis system with a metallographic
} } } } microscope. I have a few microscopes except a CCD camera. Would you
} } } } please recommend ccd cameras with low ~ medium price range. Thank you
} } } } very much in advance.
} } }
} } } I've had quite a lot of success interfacing cheap (US$200) CCTV cameras
} to
} } } microscopes. If the eyepiece is detachable from the scope, you can just
} offer
} } } up the camera without lens. If you can't remove the eyepiece, you need
} to use
} } } a lens on the camera, focussed to infinity, and as close as possible to
} the
} } } eyepiece.
} } }
} } } You can feed the composite video signal from the CCD camera to a TV (AV
} } } input), or to a video capture card (about US$100) in a computer. That
} way you
} } } can capture, store, and print the images.
} } }
} } } Quality is OK for enlargement up to about postcard size.
} } }
} } } cheers
} } }
} } } rtch
} } }
} } } Ritchie Sims Phone : 64 9 3737599 ext 7713
} } } Department of Geology Fax : 64 9 3737435
} } } The University of Auckland email : r.sims-at-auckland.ac.nz
} } } Private Bag 92019
} } } Auckland
} } } New Zealand
} }
} } --
} }
} }
} }


From daemon Mon Dec 9 17:06:15 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 09 Dec 2002 15:06:21 -0800
Subject: Re: knifemaker: CLARIFICATION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my original message concerning GKM knifemaker from Energy Beam Sciences
I mistakenly mentioned "EM Sciences" instead "EB Sciences". I deeply
apologies for that mistake. Energy Beam Sciences is current manufacturer
(?) of the GKM knifemakers and all my message was about that particular
company. I never had any problems with "EM Sciences". I am really sorry
for mistake. Sergey

At 02:23 PM 12/8/02 -0600, you wrote:
} Sergey,
}
} Bummer. I've never dealt with Energy Beam Sciences (not "EM sciences" -- a
} typo?). Just the GKM knifemaker, and to boot, this was before the
} knifemaker's name was changed to "GKM". The ones I knew were Sorvalls.
} Another company bought the product, I guess, and now a good product is
} down the tubes?
}
} I completely agree about testing equipment before buying. More
} importantly, test the company selling the product.
}
} Phil
}
} } Phil, I do agree that GKM knifemaker is good. Unfortunately I have
} } extremely bad experience with EM Sciences. When I am shopping for
} } equipment, my rule is that I have to see how equipment works before I buy
} } it (demo or exibition). Like car, you have to try it first. I was asking
} } EM Sciences for the demo. They refused. Finally after discussion on the
} } high level they did agree to sent to me instrument for demo and I'll buy
} } it if I like it... I DID sign a special 5 pages Contract (never did
} } before), they supposed to sent instrument after that. Now it's about a
} } year as they DO promise to send the instrument. When I called them a
} } month later after signing the Contract - it was a panic on the other end
} } - they were urgently assembling the instrument (they did not have any at
} } hand). So, they were lay when sign the Contract (there were exact dates
} } on the Contract for shipping). I told them, OK, I'll wait. So, I am
} } waiting and waiting... until now. I do have all paperwork from that case
} } to verify that the story is true. It looks to me that Leica is only a
} } possibility for majority of us. I really don't like old RMC knifemaker,
} } but newer try the current model. Personally I did not try 10 mm on Leica,
} } but 8 mm knifes were really good, no complains. I don't have Leica
} } instrument, I sort of wait for response from EB Sciences... meantime
} } using 10 mm W-carbide knifes from DDK. By the way, EB Sciences was
} } trying to sell to me Leica instead GKM, so they definitely have some
} } problems with GKM. I think this is a purpose of our ListServer: to share
} } experience, bad and good. I wish everyone a great Holiday Season and
} } according Russian tradition, I wish, all your wishes (at least a few of
} } them) become true in the coming New Year. Sergey
} }
} } At 05:59 AM 12/6/2002, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Dec 9 19:17:42 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 09 Dec 2002 17:06:57 -0800
Subject: Fwd: Re: GKM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Date: Mon, 09 Dec 2002 17:05:09 -0800
} To: SGKCCK-at-aol.com
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: GKM
}
} I apologize
} I already post statement on ListServer that I mentioned your company
} wrongly. I really sorry. Sincerely yours, Dr. Sergey Ryazantsev
}
} At 10:12 AM 12/7/2002, you wrote:
} } This is Stacie kirsch the owner of EM Sciences (Electron Mi8croscopy
} } Sciences). You are using our name in place of EB Sciences and we have
} } nothing to do with Glass knife makers. I would appreciate you retract
} } and make the correction so everyone understands you meant EB Sciences and
} } not EM Sciences.
} } I would really appreciate it.
} } Thanks
} } Stacie Kirsch
} } President
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Dec 9 22:48:44 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 09 Dec 2002 23:38:17 -0500
Subject: EM Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

The website for EM Science is at URL
http://www.emscience.com/

The ownership is described somewhat differently. You can see for yourself.

I was under the impression that they were part of the EM Industries Group,
an Affiliate of Merck KGaA out of Darmstadt, Germany. That is also
according to the website.

Maybe Sergey did mean EM Science after all.

Now I am myself confused.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================









------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line
Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.



} Date: Mon, 09 Dec 2002 17:05:09 -0800
} To: SGKCCK-at-aol.com
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: GKM
}
} I apologize
} I already post statement on ListServer that I mentioned your company
} wrongly. I really sorry. Sincerely yours, Dr. Sergey Ryazantsev
}
} At 10:12 AM 12/7/2002, you wrote:
} } This is Stacie kirsch the owner of EM Sciences (Electron Mi8croscopy
} } Sciences). You are using our name in place of EB Sciences and we have
} } nothing to do with Glass knife makers. I would appreciate you retract
} } and make the correction so everyone understands you meant EB Sciences and
} } not EM Sciences.
} } I would really appreciate it.
} } Thanks
} } Stacie Kirsch
} } President
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu







From daemon Tue Dec 10 09:18:05 2002



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Tue, 10 Dec 2002 10:05:09 -0500
Subject: LKB Chuck Adapter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


** Reply Requested When Convenient **

Hi Listers,

Happy Holidays!

I have an old LKB pyramitome and some old Sorvall MT2 and MT7 chucks
(with the threaded bottom). There used to be adapters out there that
you screwed onto the threaded bit that allowed you to use the Sorval
chucks in things like my LKB.

Does any one know where I can get one?

Thanks oodles & poodles in advance!

Paula :-)



Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax


From daemon Tue Dec 10 09:41:05 2002



From: Larry Hanke :      hanke-at-mee-inc.com
Date: Tue, 10 Dec 2002 09:31:57 -0600
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nikon has an adapter for the Coolpix cameras. The price was
about $600 two years ago. This adapter works in the eyepiece
tube or will attach to a C-mount adapter.

You can also make your own adapter with a 10X eyepiece with
28 mm threads to match the threads on the lens of the
Coolpix. Some of the new cheaper Coolpix cameras do not have
the threaded lens, so check for that before buying a camera
for this application.

We have used this combination for a couple of years on
various microscope with pretty good results. One major
problem with microscopy is the zoom setting on the camera
must be set to a fixed setting to get accurate
magnification, and there is no way to see what the zoom
setting is when setting up the camera.
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870




From daemon Tue Dec 10 10:19:47 2002



From: Mortro-at-aol.com
Date: Tue, 10 Dec 2002 11:08:15 -0500
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'd recommend MVIA's Coolpix adapters. We have been using them in the lab for a few months now.

We had tried unsuccessfully to get good images using our Coolpix 995 camera with an adapter from a different vendor. This summer we decided to try the adapters from MVIA. The adapters have worked great for us and we can now get good images from the camera.

Sincerely,
John Mortro



From daemon Tue Dec 10 10:31:01 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Tue, 10 Dec 2002 17:22:21 +0100
Subject: TEM Particle size measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Are other people doing particle size measurement from TEM images, and if
so how? The particles are small metal catalyst particles on carbon
supports. One problem is that automatic image processing could fail
because of the background contrast ripples from the carbon. At the
moment the student is simply measuring everything with a ruler, and this
takes ages, of course.

Secondly, does anyone have a good reference on particle size
distribution? Specifically, we would like to know how distributions are
best represented statistically, particularly when they are non-normal
(short of just showing a diagram for each one).

Best wishes
--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Tue Dec 10 10:33:03 2002



From: Qian-Chun Yu, MB, Ph.D. :      qcyu-at-mail.med.upenn.edu
Date: Tue, 10 Dec 2002 12:23:39 -0500
Subject: Environmental SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


N.B.: EM Science is not EM Sciences, necessarily. That is, EM (E. Merck)
Science, an affiliate of Merck KGaA out of Darmstadt, Germany, located in
Gibbstown, NJ, is not EM (Electron Microscopy) Sciences, aka EMS, of Fort
Washington, PA. Since we often order from both companies, I ALWAYS specify
Electron Microscopy Sciences or EM Science instead of EMS, especially since
I'm also a registered EMT volunteering in our EMS department, which has
nothing to do with EM. I won't even mention the number of times our orders
are delivered to the EM (Emergency Medicine) Department instead of our EM
(Electron Microscopy) Department. Clear now?!
(sorry Nester)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, Supervisor
Cannon Electron Microscopy Lab
Carolinas Medical Center
P.O. Box 32861; Charlotte, NC 28232-2861
Ship to: 1000 Blythe Blvd; Charlotte, NC 28203
Ofc:704-355-1267 ; Lab:704-355-7220 ; Fax 704-355-0589
E-mail: Winston.Wiggins-at-CarolinasHealthCare.org
{mailto:WWiggins-at-Carolinas.org}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


-----Original Message-----
} From: Garber, Charles A. [SMTP:cgarber-at-2spi.com]
{mailto:[SMTP:cgarber-at-2spi.com]}
Sent: Monday, December 09, 2002 11:38 PM
To: MICROSCOPY BB


Dear Colleagues,

I wish to thank all of you for the timely information regarding the
facility available at the DC-Baltimore area. I passed the information to
the friend along with my own appreciation.

Have a warm and safe Holiday Season!

QC Yu



From daemon Tue Dec 10 11:34:06 2002



From: David Knecht :      knecht-at-uconn.edu
Date: Tue, 10 Dec 2002 12:52:19 -0500
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Do you have to adjust, crop images to get the correct magnification?

Pavel

----- Original Message -----
} From: {Mortro-at-aol.com}
To: {haley-at-mvia.com} ; {atcsem-at-earthlink.net} Microscopy-at-sparc5.microscopy.com
Cc: {jfactor-at-purvid.ns.purchase.edu} ; {} ; {atcsem-at-earthlink.net}
Sent: Tuesday, December 10, 2002 11:08 AM


We have debated a Coolpix type solution, but decided against it as too
much theft potential in a multiuser lab. Anyone come up with a
solution to this dilemma? Dave

On Tuesday, December 10, 2002, at 10:31 AM, Larry Hanke wrote:

} -----------------------------------------------------------------------
} -
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} .
}
}
} Nikon has an adapter for the Coolpix cameras. The price was
} about $600 two years ago. This adapter works in the eyepiece
} tube or will attach to a C-mount adapter.
}
} You can also make your own adapter with a 10X eyepiece with
} 28 mm threads to match the threads on the lens of the
} Coolpix. Some of the new cheaper Coolpix cameras do not have
} the threaded lens, so check for that before buying a camera
} for this application.
}
} We have used this combination for a couple of years on
} various microscope with pretty good results. One major
} problem with microscopy is the zoom setting on the camera
} must be set to a fixed setting to get accurate
} magnification, and there is no way to see what the zoom
} setting is when setting up the camera.
} --
} Larry D. Hanke, P.E.
} Materials Evaluation and Engineering, Inc.
} Practical Solutions Through Technology and Innovation
} http://www.mee-inc.com (763) 449-8870
}
}
}
}
Dr. David Knecht
Department of Molecular and Cell Biology
U-125
75 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)



From daemon Tue Dec 10 14:21:00 2002



From: Jim Haley :      haley-at-mvia.com
Date: Tue, 10 Dec 2002 15:08:14 -0500
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,

Good point. My description was inaccurate.

The '30 day warranty' I spoke of is not the actual warranty period. It
is a trial period and is certainly enough time to test the adapter out
and verify its quality and determine if the adapter is satisfactory. We
offer a full refund less freight for that period - up to 30 days after
receipt of the adapters.

The actual warranty period for the adapter is one year for any defects
in workmanship, or if it breaks. Up to one year, we will
exchange/repair any adapters that break (as long as its not due to
abuse).

I can tell you that in the 2 years we have been selling the adapters we
have shipped a couple hundred adapters. Out of all these, we have not
had even 1 inquiry by anyone who wanted to return the adapter for any
reason!

Thanks & Happy Holidays!
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
125 Sherwood Drive
Monaca, PA 15061
voice: (724) 728-7493
fax: (412) 291-1709
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

"Foran, David A" wrote:
}
} "AND we stand behind them with a 30 day money back guarantee."
}
} This is more like stooping behind your product. 30 days does not give me
} much confidence in the product.
}
} -----Original Message-----
} From: Jim Haley [mailto:haley-at-mvia.com]
} Sent: Monday, December 09, 2002 4:57 PM
} To: atcsem
} Cc: jan factor; Microscopy-at-sparc5.microscopy.com; atcsem-at-earthlink.net
} Subject: Re: LM: CCD camera for LM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America To


From daemon Tue Dec 10 14:29:14 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Tue, 10 Dec 2002 15:07:06 -0600
Subject: RE: TEM Particle size measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chain it!
Just a joke. I think there is a time when we need to start trusting
coworkers.


Pavel

----- Original Message -----
} From: "David Knecht" {knecht-at-uconn.edu}
To: "microscopy" {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, December 10, 2002 12:52 PM


1. Whether digitally or in dark room try to find right
contrast for your images. Carbon film, even folded, should
have lower darkness than metal particles.
2. If this fails, try semiautomatic analysis, where you
can eliminate unwonted features from each field of view.
3. If you have access to a SEM and your particles are big
enough, then the easiest way is to analyze images obtained
with a backscattered detector.

Regards,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
} Sent: Tuesday, December 10, 2002 10:22 AM
} To: Microscopy
} Subject: TEM Particle size measurement
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear all,
} Are other people doing particle size measurement from TEM
} images, and if
} so how? The particles are small metal catalyst particles on carbon
} supports. One problem is that automatic image processing could fail
} because of the background contrast ripples from the carbon. At the
} moment the student is simply measuring everything with a
} ruler, and this
} takes ages, of course.
}
} Secondly, does anyone have a good reference on particle size
} distribution? Specifically, we would like to know how
} distributions are
} best represented statistically, particularly when they are non-normal
} (short of just showing a diagram for each one).
}
} Best wishes
} --
} Ian MacLaren
} Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
} ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/
}
}
}


From daemon Tue Dec 10 16:48:24 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 10 Dec 2002 15:28:54 -0700
Subject: TEM Particle size measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can you remove the background through a shading correction? If your
background variation is very different from your particles (background low
frequency fluctuations on a larger scale than the particles), I would
recommend to try unsharp masking. Take the image, run an NxN filter on the
image, then subtract from original image. You can play with the filter size
for best results.

As for display: automatically detect the particles after the correction
above and measure the parameter you want to display. You will then have to
define a classification, and then a simple histogram of the results should
give you the diagram you want. Other than that you could evaluate higher
order statistical parameters from the results (such as kurtosis), and
specify those. Our software has those as standard statistical parameters,
and I would assume other software does as well.

If you send me an image, I can run that here and send you the results.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
Sent: Tuesday, December 10, 2002 9:22 AM
To: Microscopy


Dear all,
Are other people doing particle size measurement from TEM images, and if
so how? The particles are small metal catalyst particles on carbon
supports. One problem is that automatic image processing could fail
because of the background contrast ripples from the carbon. At the
moment the student is simply measuring everything with a ruler, and this
takes ages, of course.

Secondly, does anyone have a good reference on particle size
distribution? Specifically, we would like to know how distributions are
best represented statistically, particularly when they are non-normal
(short of just showing a diagram for each one).

Best wishes
--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Tue Dec 10 19:42:05 2002



From: hmeeks :      hmeeks-at-dc.rr.com
Date: Tue, 10 Dec 2002 17:29:22 -0800
Subject: Southern CA Employment/Mgr. Clinical and Scientific Product Support

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






Manager, Clinical and Scientific Product Support

Advanced Medical Optics, Inc.

www.amo-inc.com


The Company

Advanced Medical Optics, Inc. (AMO) located in Orange County California, is
the world's second largest surgical company in the markets in which it
competes, and the world's second largest contact lens care company. AMO is
currently the world's fastest growing company in the sale of foldable
intraocular lenses for cataracts. AMO possesses well-known products and
brands (such as Complete, Lens Plus, Array, Sensar and Sovereign), superb
technology, and experienced entrepreneurial management.

The Position

This position will be both the scientific and clinical spokesperson for AMO
and contact lens care products. It will develop and provide research for
marketing support of contact lens care products and maintain relationships
with key practitioners and educational and research institutions.

The Requirements

Candidates should have Doctoral degree (Ph.D., O.D., M.D.) and approximately
six years experience in Ophthalmic/Contact Lens research or a Masters degree
in biological or health-related science with nine years experience. Must
have successfully demonstrated public speaking skills to audiences of
healthcare professionals. Should have a working knowledge of experimental
design, laboratory environment, statistical data analysis and ophthalmic
clinical practice.

For consideration email resume to: hmeeks-at-dc.rr.com





From daemon Tue Dec 10 21:23:49 2002



From: Mortro-at-aol.com
Date: Tue, 10 Dec 2002 22:14:57 -0500
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No, we don't have to crop. On some of the scopes, we adjust the zoom properly on our camera first before taking pictures but it's always the same for each scope.


From daemon Tue Dec 10 21:24:23 2002



From: Ron L'Herault :      lherault-at-bu.edu
Date: Tue, 10 Dec 2002 22:16:19 -0500
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




We have been using the adaptor Jim sells along with two other microscope
adaptors he supplied for about a year now. We are very pleased with
them. We can now put the Coolpix on our hardness tester, tissue culture
microscope, metallurgical microscope, the dissecting microscope and our
compound microscope. Fantastic.

Ron L


-----Original Message-----
} From: Jim Haley [mailto:haley-at-mvia.com]
Sent: Tuesday, December 10, 2002 3:08 PM
To: Foran, David A; Microscopy-at-sparc5.microscopy.com


David,

Good point. My description was inaccurate.

The '30 day warranty' I spoke of is not the actual warranty period. It
is a trial period and is certainly enough time to test the adapter out
and verify its quality and determine if the adapter is satisfactory. We
offer a full refund less freight for that period - up to 30 days after
receipt of the adapters.

The actual warranty period for the adapter is one year for any defects
in workmanship, or if it breaks. Up to one year, we will
exchange/repair any adapters that break (as long as its not due to
abuse).

I can tell you that in the 2 years we have been selling the adapters we
have shipped a couple hundred adapters. Out of all these, we have not
had even 1 inquiry by anyone who wanted to return the adapter for any
reason!

Thanks & Happy Holidays!
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
125 Sherwood Drive
Monaca, PA 15061
voice: (724) 728-7493
fax: (412) 291-1709
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

"Foran, David A" wrote:
}
} "AND we stand behind them with a 30 day money back guarantee."
}
} This is more like stooping behind your product. 30 days does not give
me
} much confidence in the product.
}
} -----Original Message-----
} From: Jim Haley [mailto:haley-at-mvia.com]
} Sent: Monday, December 09, 2002 4:57 PM
} To: atcsem
} Cc: jan factor; Microscopy-at-sparc5.microscopy.com; atcsem-at-earthlink.net
} Subject: Re: LM: CCD camera for LM
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America To






From daemon Tue Dec 10 21:25:32 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Wed, 11 Dec 2002 08:15:10 -0000
Subject: Re: TEM Particle size measurement

Contents Retrieved from Microscopy Listserver Archives
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We keep ours in a locked drawer when not in use. Only the technicians
have the combination. Of course, locks only keep out honest people.

Ron L

-----Original Message-----
} From: atcsem [mailto:atcsem-at-earthlink.net]
Sent: Tuesday, December 10, 2002 2:30 PM
To: David Knecht
Cc: microscopy-at-sparc5.microscopy.com


Chain it!
Just a joke. I think there is a time when we need to start trusting
coworkers.


Pavel

----- Original Message -----
} From: "David Knecht" {knecht-at-uconn.edu}
To: "microscopy" {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, December 10, 2002 12:52 PM


Ian
The mean, standard deviation, standard error of the mean, skewness and
kurtosis would provide a good description
of the distribution, the latter two being measures of two kinds of
departure from normality.
All of these can be calculated using Microsoft Excel
(Do Insert _ Function_ Statistical _ AVERAGE, STDEV,
STDEV/SQRT(N),SKEW, KURT, )
If you want a diagram then a frequency distribution plot
can be calculated (Do Tools_ Data Analysis_ HISTOGRAM).
These functions are also fairly automatic in most statistical analysis
packages such as SigmaStat, SPSS, Minitab, some of which will plot a
normal distribution curve on top of the histogram.
Chris

} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de]
} Sent: Tuesday, December 10, 2002 9:22 AM
} To: Microscopy
} Subject: TEM Particle size measurement
}
}
} --------------------------------------------------------------------
----
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
---.
}
}
} Dear all,
} Are other people doing particle size measurement from TEM images,
and if
} so how? The particles are small metal catalyst particles on carbon
} supports. One problem is that automatic image processing could fail
} because of the background contrast ripples from the carbon. At the
} moment the student is simply measuring everything with a ruler, and
this
} takes ages, of course.
}
} Secondly, does anyone have a good reference on particle size
} distribution? Specifically, we would like to know how distributions
are
} best represented statistically, particularly when they are
non-normal
} (short of just showing a diagram for each one).
}
} Best wishes
} --
} Ian MacLaren
} Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
} ian.maclaren-at-physics.org /
http://homepages.tu-darmstadt.de/~maclaren/
}
}



From daemon Wed Dec 11 07:49:08 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 11 Dec 2002 08:39:04 -0500
Subject: Hummer sputter coaters

Contents Retrieved from Microscopy Listserver Archives
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List members,
We have an old Hummer coater that was marketed by the Technics Company.
I would like to find out if this company is still in business and, if so,
where they are now located.
Also, who is presently handling the Hummer coater line or who has been
in the recent past. I know there were a number of other models produced
since ours was purchased in the early 80's.
Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Wed Dec 11 07:49:09 2002



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Wed, 11 Dec 2002 08:39:05 -0500
Subject: Re: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List:
Does anyone have experience with Nikons new low end professional digital SLR
offering, model D100? It uses a standard Nikon SLR body and mount so most
of their lenses fit and auto features will work with many of the lenses (AF
& D type that we use). It is a 6 mega pixel camera listing for ~$2,000
(body only). Since we currently use Nikon SLR camera's on our OM systems
and have several Nikon lenses for copy stand work this seems like a very
good fit. Considering all of the costs associated with purchasing and
mounting fixed lens consumer camera's onto microscopes it appears to be a
viable option? True the D100 costs are slightly higher however the benefits
are significant, high resolution images, selectable lens ranges, improved
lens quality, versatile camera software, and I am assuming it uses standard
microscope mounting hardware found in many labs.

What have I missed? Are there pitfalls associated with this camera? Since
this is a newer camera model I can not confirm some of the microscope
mounting information. Please correct any errors or misinformation above.
Any additional input or insight would be appreciated.

Sincerely, jr



John Robson
Boehringer Ingelheim Pharmaceuticals, Inc.
PO Box 368
900 Ridgebury Rd
Ridgefield, CT 06877

Phone (203)798-5640
Fax (203)798-5698

e-mail jrobson-at-RDG.boehringer-ingelheim.com


-----Original Message-----
} From: "Mortro-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Mortro-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 10, 2002 11:08 AM
To: haley-at-mvia.com; atcsem-at-earthlink.net
Cc: jfactor-at-purvid.ns.purchase.edu; Microscopy-at-sparc5.microscopy.com;
atcsem-at-earthlink.net


I'd recommend MVIA's Coolpix adapters. We have been using them in the lab
for a few months now.

We had tried unsuccessfully to get good images using our Coolpix 995 camera
with an adapter from a different vendor. This summer we decided to try the
adapters from MVIA. The adapters have worked great for us and we can now
get good images from the camera.

Sincerely,
John Mortro





From daemon Wed Dec 11 07:54:26 2002



From: David Burton :      dburton-at-nwlink.com
Date: Wed, 11 Dec 2002 05:48:23 -0800
Subject: Re: Coolpix security

Contents Retrieved from Microscopy Listserver Archives
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} Chain it!
} Just a joke. I think there is a time when we need to start trusting
} coworkers.

Unfortunately it is not just our coworkers. Many labs have students,
janitorial workers, maintenance staff and casual passer-bys who need only a
moment of opportunity to steal something. As soon as the microscope
manufacturers started putting consumer cameras on microscopes we started
losing cameras to theft. This was never a problem with the dedicated
microscope cameras which could not be used for other purposes. A researcher
at our University just had a digital consumer camera stolen from his
microscope along with irreplaceable data stored on the memory card. Our
pathology department had an entire video microscope system stolen from the
conference room by a student. How it was found in his locker months later
by the police I don't know. I suspect he had been involved in other thefts.
This department now has anti-theft devices on all of their microscopes.
These are either glued on pads with a security cable and lock or an eyebolt
attached to the microscope body with a cable and lock.
I have mounted a small diameter cable on a Coolpix camera attached to
the neck strap attachment point. The tripod socket could also be used as an
attachment point. The cable is attached to the microscope with a lock and is
long enough that the camera can be moved between several scopes.

If someone is determined to steal something there is almost nothing we
can do to stop them, and if the security measures become too onerous people
will stop using them. It has been our experience though that it only takes
a minor deterrent to prevent most thefts.

David Burton
Optical Specialist
University of Washington



From daemon Wed Dec 11 09:53:45 2002



From: michael shaffer :      michael-at-shaffer.net
Date: Wed, 11 Dec 2002 12:11:25 -0330
Subject: RE: about coolpix adaptors

Contents Retrieved from Microscopy Listserver Archives
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Jim Haley writes ...

} ...
} The '30 day warranty' [...] is certainly enough time
} to test the adapter out and verify its quality and
} determine if the adapter is satisfactory. ...

Before we order, or request a trial, how do we best determine what type of
'C-mount' is required. When I looked into this more than a year ago, a 1X
would have been best for a 9xx Coolpix and its CCD. Is there a formula for
determining the mag factor of the C-mount adapter relative to the size of
the CCD in the camera???

Also ... is the adapter different with regard to older Nikon 9xx and newer
5xxx cameras??

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)




From daemon Wed Dec 11 10:16:38 2002



From: Anjeanette Ormonde :      Anjeanette.Ormonde-at-unilever.com
Date: Wed, 11 Dec 2002 10:08:12 -0600 (Central Standard Time)
Subject: Suggest a dye?

Contents Retrieved from Microscopy Listserver Archives
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Hello,
Does anyone know of a fluorescent dye that would specifically tag/stain
silicone oil droplets in complex mixtures or suspensions? Thanks in
advance for any options you can offer!

Angie


From daemon Wed Dec 11 10:24:05 2002



From: Leslie Eibest :      leibest-at-duke.edu
Date: Wed, 11 Dec 2002 11:19:54 -0500
Subject: Re: Hummer Sputter coaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Debbie,

The Hummer line is now carried by Anatech Ltd. Their address is 6621-F
Electronic Drive, Springfield, VA 22151-4303. Phone # is (800) PLASMA-9,
their home page is at http://www.anatechltd.com.


You wrote:


} We have an old Hummer coater that was marketed by the Technics Company.
} I would like to find out if this company is still in business and, if so,
} where they are now located.
} Also, who is presently handling the Hummer coater line or who has been
} in the recent past. I know there were a number of other models produced
} since ours was purchased in the early 80's.

Leslie Eibest
SEM lab manager
Dept. of Biology
Duke University



From daemon Wed Dec 11 10:43:33 2002



From: Franklin Bailey :      jfb-at-uidaho.edu
Date: Wed, 11 Dec 2002 08:37:43 -0800
Subject: AMRAY Service

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know of a service company in the Pacific Northwest area who
provides maintenance for AMRAY SEMs? I would be grateful for the names
and email/phone/snailmail addresses for them. Thank you.

Franklin Bailey
Electron Microscopy Center
University of Idaho
Moscow, ID 83844-2204
jfb-at-uidaho.edu



From daemon Wed Dec 11 11:15:23 2002



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 11 Dec 2002 18:03:45 +0100
Subject: RE: LM: CCD camera for LM

Contents Retrieved from Microscopy Listserver Archives
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Keep care at the size (in mm) of the CCD device. A collegue has bought a
Fuji ??? (I don't remember the reference, it changes so fast !) with
F-mount for Nikon optics two years ago, and the CCD is something like
18x25mm. All the optics are shifted in field angle. He uses now a 35mm as
"normal" focal and so on. It's not a problem in practice, but if you have
a "good" set of optics for 24x36mm, it is not automaticaly that you want
with an other device size, with some difficulty to find a wide field which
fits (typically a 15 mm).

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From daemon Wed Dec 11 11:18:01 2002



From: Chris Edwards :      fishon-at-umich.edu
Date: Wed, 11 Dec 2002 12:10:27 -0500
Subject: Leafscan-45 bulb

Contents Retrieved from Microscopy Listserver Archives
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Does anyone out there know where one might get a replacement bulb for the
Leafscan-45 film scanner? Thanks.

Chris A. Edwards, Mgr.
Microscopy and Image-analysis Laboratory
Dept. Cell & Developmental Biology
The University of Michigan, School of Medicine
4747 Medical Sciences II Bldg.
Ann Arbor, Michigan 48109-0616
Office: 734-936-4912
Lab: 734-763-1170
FAX: 734-763-1166




From daemon Wed Dec 11 14:50:28 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 11 Dec 2002 15:29:06 -0500
Subject: WILD illuminator Cords

Contents Retrieved from Microscopy Listserver Archives
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I have now 3 WILD dissecting (stereo compound ;) ) microscope
illuminators with cords that do not work. The problem is in the cord
from the bulb to the power supply.
This is a 6VAC System.

The two prong plug seems to be unique relative to all the other plugs
around the Department and here in my lab.

Question: Does anyone know of a supplier for these cords with the two
round prongs? This past summer when the last one died I attempted to
contact suppliers and came up empty handed but the situation wasn't
critical so I moved on. Now it has become critical and this is about
the last line of hope I have to get these working again.

The two prongs (both round) are 4mm in diameter and the outside to
outside distance is 20mm (inside distance:12mm, c-c:16mm)

Thank you in advance
Geoff Williams
Microscopy Facility Supervisor
 
Checkout the new Biology Department Microscopy Facility web page. 
Version 1 is now On-Line:
www.cst.cmich.edu/users/willi1gl/BDMF/BDMF.htm




From daemon Wed Dec 11 16:26:51 2002



From: Calvert, Dave - Voridian :      dcalvert-at-voridian.com
Date: Wed, 11 Dec 2002 17:17:46 -0500
Subject: RE: Suggest a dye?

Contents Retrieved from Microscopy Listserver Archives
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Angie - You might try Nile Red to "stain" the oil phase in an emulsion.
I have used Nile Red in lubrication studies, dissolving the dye (25ppm will
do) in the oil phase before making up the emulsion.
Omega recommends their X33 filter - "Wide-band TRITC set".
As I remember the lit, you needn't be concerned about solubility of Nile
Red in any aqueous phase because the dye will only fluoresce significantly
in a hydrophobic environment.

Dave Calvert
Voridian Division
Eastman Chemical Co.
P.O. Box 1972
Kingsport, Tennessee
Voice: 423-229-4943
Fax: 423-224-7550


Hello,
Does anyone know of a fluorescent dye that would specifically
tag/stain
silicone oil droplets in complex mixtures or suspensions? Thanks in
advance for any options you can offer!

Angie


From daemon Wed Dec 11 17:38:21 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Wed, 11 Dec 2002 18:26:40 -0500
Subject: digital image capture systems

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,
I have a friend who has about 10 customers looking for a digital image
capture system for their SEMs. I know several vendors are frequenters
of this list, but I'm not at all sure that it's inclusive. Does anyone
have a really complete list of digital image capture vendors, perhaps
even broken down by active vs. passive systems?

Thanks,
Ken Converse
owner
Quality Images
third party SEM service
Delta, PA




From daemon Wed Dec 11 21:14:59 2002



From: luciana delgado :      luciana.delgado-at-upct.es (by way of
Date: Wed, 11 Dec 2002 21:05:05 -0600
Subject: information on quantify cell area from a SEM image

Contents Retrieved from Microscopy Listserver Archives
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Dear Mrs/Mr,

I am looking for a program to quantify cell area from a SEM image.

I would like to know if you have some tool that could be useful for
my objective.

Thank you for everything, I will be waiting for your answer.

Lucian Delgado Benarroch

Universidad PolitŽcnica de Cartagena.

Murcia. Espa–a


From daemon Thu Dec 12 05:28:03 2002



From: sandy bond :      tinacdn-at-abanet.it
Date: Wed, 11 Dec 2002 15:50:30 -1700
Subject: Product of the year awaits you inside

Contents Retrieved from Microscopy Listserver Archives
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Here's the hottest thing in DVDs. Now you can make a personal backup
copy of a DVD right onto CD-R. Our "Hot" new software easily takes you through
the steps to make a copy of your own DVDs.

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or offers. If you do not wish to receive any further messages from AB Network.

http://aim.wesellforyou.net/pos/



From daemon Thu Dec 12 10:05:05 2002



From: Nick SCHRYVERS :      schryver-at-ruca.ua.ac.be
Date: Thu, 12 Dec 2002 16:54:32 +0100
Subject: conference announcement

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,

The next European Microscopy Congress, EMC 2004 (sequel of the EUREM
series, last meeting in Brno, 2000), will be held in Antwerp, Belgium,
from August 22 till 27, 2004. All members of European societies will
recieve a hardcopy of the first circular.

The website www.emc2004.be contains all available information and will
be regularly updated. If you are interested to recieve a hardcopy of the

second circular, due September 2003 and containing all information on
registration, abstract submission, accommodation, etc. you can fill in
the on-line Expression of Interest form for Scientific Delegates.

Commercial Exhibitors are invited to fill in the Expression of Interest
form for Exhibitors and Sponsors, after which you will recieve
additional information as of April 2003.

We look forward to see you in Antwerp,

Nick Schryvers
President EMC 2004


--

Visit http://www.emc2004.be , the official site of the next European
Microscopy Congress in 2004

******************************************************

Prof. Dr. Dominique Schryvers

Professor of Physics

Electron Microscopy for Materials Research (EMAT)

Department of Physics

University of Antwerp, RUCA

Groenenborgerlaan 171

B-2020 Antwerp, Belgium

Tel: 32-3-2180247

Fax: 32-3-2180257

E-mail: schryver-at-ruca.ua.ac.be, nick.schryvers-at-ua.ac.be
{



From daemon Thu Dec 12 11:04:06 2002



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Fri, 01 Jan 1904 00:30:45 -0600
Subject: Service for ElectroScan ESEMs

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


My lab has inherited an ElectroScan 2020 ESEM. Does anyone know of any
service companies in the Southern US that provide maintenance service
for ElectroScan SEMs. If so, please email me their contact
information. Thank you.
Ty

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Facility
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Thu Dec 12 15:38:08 2002



From: Jeannette Taylor :      jvtaylo-at-emory.edu
Date: Thu, 12 Dec 2002 16:25:50 -0500
Subject: technique

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Message-ID: {3DF8FEDD.79FF5B22-at-emory.edu}


Dear List , I have a question. How does on go about punching out a disc
of cultured cells on a plastic culture dish for the purpose of a TEM
study?
Without damaging the cells, of course. If possible please give me some
references so I can read about it. Please reply to my e-mail address,
below.
I have been surfing web references but have as yet not found any for
this specific technique.

thanks, Jeannette Taylor
jvtaylo-at-emory.edu



From daemon Thu Dec 12 18:19:32 2002



From: K.N. Bozhilov :      bozhilov-at-citrus.ucr.edu
Date: Thu, 12 Dec 2002 16:06:21 -0800
Subject: EMSCOPE

Contents Retrieved from Microscopy Listserver Archives
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We have an EMSCOPE SC500 sputter coater which needs repair. As far as I know
EMSCOPE is out of business and I am searching for possibilities to repair
the unit. Any leads or suggestions?

Thank you,

Krassimir.
______________________________
Dr. Krassimir Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel.: 909 787 2998
Fax: 909 787 4324
______________________________



From daemon Thu Dec 12 18:58:37 2002



From: Steve Barlow :      sbarlow-at-sciences.sdsu.edu
Date: Thu, 12 Dec 2002 16:48:56 -0800
Subject: attaching granulocytes

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A user wants to stick down granulocytes. Any suggestions on ways to
do this? The user sticks down monocytes with polylysine no problem.
Any reason why this polylysine procedure is not working for her
granulocytes?

Steve
--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/


From daemon Fri Dec 13 05:01:24 2002



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 13 Dec 2002 10:51:21 +0000 (GMT Standard Time)
Subject: Re: EMSCOPE

Contents Retrieved from Microscopy Listserver Archives
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I think Emscope staff went to Polaron and Emitech. I have
the Emitech UK address if you cannot find a local firm.

Dave

On Thu, 12 Dec 2002 16:06:21 -0800 "K.N. Bozhilov"
{bozhilov-at-citrus.ucr.edu} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} We have an EMSCOPE SC500 sputter coater which needs repair. As far as I know
} EMSCOPE is out of business and I am searching for possibilities to repair
} the unit. Any leads or suggestions?
}
} Thank you,
}
} Krassimir.
} ______________________________
} Dr. Krassimir Bozhilov
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} Tel.: 909 787 2998
} Fax: 909 787 4324
} ______________________________
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Dec 13 07:46:07 2002



From: Leslie Eibest :      leibest-at-duke.edu
Date: Fri, 13 Dec 2002 08:39:20 -0500
Subject: Re: service for Electroscan ESEM

Contents Retrieved from Microscopy Listserver Archives
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Hi Ty,

FEI (who purchased Philips Electron Optics, who purchased Electroscan) can
provide service. The head of SEM service is Scott Shawmeker
(sshawmeker-at-feico.com, I think), but he may be out of the country. If you
contact the FEI call center at (800) 432-1734, they can direct you to
someone who can help you.

You wrote:

} My lab has inherited an ElectroScan 2020 ESEM. Does anyone know of any
} service companies in the Southern US that provide maintenance service
} for ElectroScan SEMs. If so, please email me their contact
} information. Thank you.


Leslie Eibest
SEM lab manager
Dept. of Biology
Duke University



From daemon Fri Dec 13 07:51:20 2002



From: mike.wombwell-at-quorumtech.com (by way of MicroscopyListserver)
Date: Fri, 13 Dec 2002 07:46:03 -0600
Subject: EMSCOPE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr.Bozhilov,

We (Quorum Technologies) continue to support the original Emscope SC500. For
local support and service please contact Energy Beam Science (EBS) our
distributors in the US:

Energy Beam Sciences
11 Bowles Road
PO Box 468
Agawam
MA 01001
Tel: 413 786-9322
Fax: 413 789-2786
sales-at-ebsciences.com
http://www.ebsciences.com

If you need an operating manual this can be downloaded from the Quorum
Technologies website (www.quorumtech.com)

Just a little history. The original UK based Emscope company was sold to
Bio-rad Microscience (then the manufacturers of the Polaron range) in 1988.
The Polaron / Emscope range was then acquired by VG Microtech (now called
Thermo VG Scientific) in 1991, and finally, Quorum Technologies was founded
in 2001 following the purchase of the Polaron range from Thermo VG.

Best regards,

Mike Wombwell

****************************************************************************
**********************************************
Quorum Technologies
Unit 15A, Euro Business Park
New Road, Newhaven
East Sussex, BN9 0DQ, UK
Tel: +44(0)1273 510535 (main switch board)
Tel: +44(0)1273 510620 (direct line)
Mobile +44(0)7989 426 431
Fax: +44(0)1273 510536
mike.wombwell-at-quorumtech.com
http://www.quorumtech.com
http://www.sputtercoater.com
http://www.criticalpointdryer.com
E & O E


-----Original Message-----
} From: K.N. Bozhilov [mailto:bozhilov-at-citrus.ucr.edu]
Sent: 13 December 2002 00:06
To: microscopy-at-sparc5.microscopy.com


We have an EMSCOPE SC500 sputter coater which needs repair. As far as I know
EMSCOPE is out of business and I am searching for possibilities to repair
the unit. Any leads or suggestions?

Thank you,

Krassimir.
______________________________
Dr. Krassimir Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel.: 909 787 2998
Fax: 909 787 4324
______________________________


From daemon Fri Dec 13 08:25:09 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 13 Dec 2002 08:15:34 -0600
Subject: attaching granulocytes

Contents Retrieved from Microscopy Listserver Archives
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Steve,

We recently had a similar problem with oocytes. We finally salvaged some of them by using extended times for adhering them to polylysine cover slips, but our initial try with one hour adhesion times didn't work well.

Although we haven't yet tried it for this purpose, there is an adhesive called Mikrostik which we intend to try in the future. It is available from Ted Pella (Cat. no. 16033) and works well for adhering particulates without engulfing them in glue.

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---The Fun Core!!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Steve Barlow [mailto:sbarlow-at-sciences.sdsu.edu]
Sent: Thursday, December 12, 2002 6:49 PM
To: microscopy-at-sparc5.microscopy.com


A user wants to stick down granulocytes. Any suggestions on ways to
do this? The user sticks down monocytes with polylysine no problem.
Any reason why this polylysine procedure is not working for her
granulocytes?

Steve
--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/



From daemon Fri Dec 13 08:36:06 2002



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Fri, 13 Dec 2002 15:31:22 +0100
Subject: Re: attaching granulocytes

Contents Retrieved from Microscopy Listserver Archives
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Hi

I can't answer the question about why it works for one type of cell but not
the other - polylysine works on charge which should be the same for them.

Are the cells in the same sample? Have they been treated in the same way?
What are the conditions of temperature, fixation, buffer etc.

What is the sample?

Has she tried superfrost plus?

More questions than answers!

Have a good weekend!

Nadolig Llawen a Blwddyn Newydd Dda / God Jul och Gott Nytt År / Merry
Christmas and a Happy New Year
Obs/NB New postal/visiting address from July 2002!

Med vänliga hälsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 5858 1038
Fax +46 728 3747

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Huddinge Universitetssjukhus, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.



From daemon Fri Dec 13 08:50:55 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 13 Dec 2002 09:38:21 -0500
Subject: Re: technique

Contents Retrieved from Microscopy Listserver Archives
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Hi Jeanette,
I have processed cell cultures in dishes with great success with the
following protocol:
Wash culture with serum-free medium at 37 deg. C
Remove medium & fix cells with your primary fix, also at 37 deg. (I
use 2.5% glut + 4% pfa + 0.002% picric acid in 0.1M sodium
cacodylate, but you should use whatever you wish). The cells will fix
almost instantly, but I usually leave them for about 30 min.
wash with buffer of choice.
Post-fix with osmium 30-60 min.
Wash with buffer.
Dehydrate using a graded ethanol series.
DO NOT USE Propylene oxide...it eats the dishes!
Here's where things get slightly different:
I embed using a pretty standard "Epon" with the exception that I use
LX112 and DMP-30 from Ladd, and DDSA and NMA from EMS.
LX112 9.7 gm
DDSA 3.2 gm
NMA 5.9 gm
DMP-30 17 microliter/ml

To embed: Cut the tips off of BEEM capsules to make tubes. Cover the
bottom of the dish with a thin layer (about 1-2 mm thick) of the
resin and stand the tubes in the resin, over areas of interest if
there are any. Polymerize overnight. In the morning top off JUST
the BEEM tubes and return to the oven for another day. When you take
the dish out, you will be able to grab the BEEM tubes with a pair of
needle-nosed pliers and snap them out. Your cells will be on the
block face. I usually also get a small amount of the dish, but that
separates easily when I trim the blocks.
Of course, this will give you en face sections of the cells. If you
need vertical sections, cut a wafer off the front of the block &
reembed it.

Call if you have any questions.
good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Dec 13 09:04:53 2002



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Fri, 13 Dec 2002 09:53:58 -0500
Subject: Re: technique

Contents Retrieved from Microscopy Listserver Archives
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Jeannette,
We routinely process cultured cells for TEM per the following protocol:

Falcon and Corning dishes seem to work better than CoStar if you have a choice.
Fix, wash, osmicate, wash and dehydrate with ethanol as usual but with
shorter times since you have a monolayer.
Do not use propylene oxide as it will dissolve the dish.
After the 100% ethanol step, pour all the alcohol off the sample and put
resin on. Use an epoxy type resin such as LX 112, eponate 12 or epon 812,
but not Spurr's.
Do NOT use a 50/50 mixture of alcohol and resin. (This combination appears
to partially dissolve the dish while the components used separately do not.)
Change the resin 2 times over a period of 4 hours.
Pour off the resin.
Fill beam capsules with resin and invert them on the sample. If the dish is
small a hemostats are useful for inverting the capsules.
Polymerize for 36 hrs at 60 degrees. Let cool and pop blocks off the
dish. The cells will come off the dish cleanly with no damage.

Call or email me if you have questions and good luck.
Mary Gail Engle



PourAt 04:25 PM 12/12/02 -0500, Jeannette Taylor wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-9700


From daemon Fri Dec 13 09:07:31 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 13 Dec 2002 10:00:45 -0500
Subject: Emscope product support

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

David Patton wrote:
===========================================
I think Emscope staff went to Polaron and Emitech. I have
the Emitech UK address if you cannot find a local firm.
=========================================
At one time there was a free-standing company (founded by Dr. Gerald Kaye)
in Watford, England called Polaron Ltd. In around 1982 the company was
acquired by BioRad Laboratories, Inc. and it became part of the BioRad
Microscience Division. At about that time, either before or after the
acquisition (my memory now starts to fade) but under Dr. Kaye's tenure, the
firm acquired Emscope. So the entire Emscope operation was absorbed into
what the marketplace then knew as Polaron.

With the passing of years, the Polaron part (or what became known as the
Polaron EM Prep Lab business) was sold off (by BioRad) to Fisons Instruments
(VG MIcrotech Division), then that whole part of Fisons was acquired by
Thermo Instruments and about two years ago, what was left of the original
Polaron, was spun out and acquired by the senior management of the unit and
is now called

Quorum Technologies
Unit 15A
Euro Business Park
New Road
Newhaven
East Sussex
BN9 0DQ
England
Tel (+44) 01273 510535
Fax (+44) 01273 510536

My main contact there is Mr. Mike Wombwell (mike.wombwell-at-quorumtech.com).

They continue to manufacture the Polaron line of equipment and much to my
great pleasure, they are maintaining the tradition of supplying parts for
old instruments. We had a need for a replacement transformer for a vacuum
evaporator that was manufactured in about 1985 but getting it was no problem
. Some of the current Quorum products have progenies that I think can be
traced back to some of the old Emscope products.

So if you need a replacement part for an old Emscope unit, contact Quorum
Technologies.

Disclaimer: SPI Supplies has no vested interest in Quorum, however we do
purchase certain items from time to time from them (and they from us).
They are our competitor and a highly reputable one at that!

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Fri Dec 13 09:09:06 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 13 Dec 2002 10:01:48 -0500
Subject: Re: technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeanette,
Do you want to remove the cells before or after fixation & embedding?
If before, have them put some thermolux plastic cover slips into the culture
dishes and then you can easily remove one to fix it without damaging the
rest of the culture.

If you want to fix and embed in the culture dish then you can do one of a
number of things:

A) X-section of culture: Add enough embedding resin (must infiltrate with
resin + ETOH not Propylene oxide or acetone which will dissolve the
plastic...this will work with EPON generics) to make a thickness equivalent
to that of a flat embedded section. Later break away the petri dish and cut
out pieces of the embedded sample with a jewelers saw for microtomy.

B) Horiz. section: Polymerize with a slightly thinner amount of resin so
that you can still break the dish away, easily cut small pieces of the
culture and attach them to specimen blocks for microtoming.

C) Horiz section: Polymerize cells with a very thin coat of resin. While
this is polymerizing place beem or gelatin capsules, that have had their
ends cut off, over the cells of interest so that the capsules will be
secured to the dish during polymerization. Fill the capsules up with resin
and again polymerize. Break the specimen capsule away from the dish
bringing with it part of the culture.

If you need to check the cells prior to selecting ones to cut, you can
polymerize the culture with the very thin resin layer and then examine it
under a microscope to select your cell areas. Then place the capsules over
those areas, add a drop of resin and let polymerize. Once capsules are
sealed to the surface you can fill them up and again polymerize.

This is a very easy method to use to make multiple blocks without need
to do lots of sawing and cutting hard resins. You can do similar things with
cells grown on coverslips.

If orientation isn't important, just fix cells in culture dishes, scrap off
and pellet using a little agarose to hold the pellet together if necessary
so it can be cut into small "blocks", dehydrate, infiltrate, embed and you
are all set.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907


On 12/12/02 4:25 PM, "Jeannette Taylor" {jvtaylo-at-emory.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear List , I have a question. How does on go about punching out a disc
} of cultured cells on a plastic culture dish for the purpose of a TEM
} study?
} Without damaging the cells, of course. If possible please give me some
} references so I can read about it. Please reply to my e-mail address,
} below.
} I have been surfing web references but have as yet not found any for
} this specific technique.
}
} thanks, Jeannette Taylor
} jvtaylo-at-emory.edu
}
}
}



From daemon Fri Dec 13 09:13:02 2002



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Fri, 13 Dec 2002 10:05:35 -0500
Subject: technique

Contents Retrieved from Microscopy Listserver Archives
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Jeanette:

I wouldn't punch our a disc from plastic culture dishes. Different plastic
dishes can partially dissolve depending on the embedding media used.

Tell the researcher that the best way to look at cultured cells for TEM is
to use ACLAR Embedding Film from EM Sciences. It can be cut to size to fit
in a petri dish and the cells easily attach. Then you can process the
Aclar/cells as if normal its a normal tissue sample, embedding it the film
with the cells face down in a mold. It's a rare cell type that won't grow
on these sheets, so there should be no problem to plate cells onto the
ACLAR.

You can buy the ACLAR embedding film from Electron Microscopy Sciences, look
on page 140 in their catalog.

A second method would be to have the researchers grow cells on thick glass
coverslips and "pop-off" the cells after embedding and polymerization of the
epoxy resin. If you want that technique email me directly.

Good Luck!

Karen Bentley

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954


-----Original Message-----
} From: Jeannette Taylor [mailto:jvtaylo-at-emory.edu]
Sent: Thursday, December 12, 2002 4:26 PM
To: Microscopy


Dear List , I have a question. How does on go about punching out a disc
of cultured cells on a plastic culture dish for the purpose of a TEM
study?
Without damaging the cells, of course. If possible please give me some
references so I can read about it. Please reply to my e-mail address,
below.
I have been surfing web references but have as yet not found any for
this specific technique.

thanks, Jeannette Taylor
jvtaylo-at-emory.edu



From daemon Fri Dec 13 09:34:15 2002



From: saram-at-duke.edu
Date: Fri, 13 Dec 2002 10:22:52 -0500 (EST)
Subject: Re: technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Do you mean just taking a few and leaving the rest on the plate (why would
you want to "punch out" the cells?)?

There are several ways to embed adherent cells in tissue culture plates.
Tell us more about what you want to do, and we'll try to help.

Also, see
Miller SE. 1985. Electron microscopy of tissue culture. In Jones BR,
Electron Microscopy: 41 Exercises by 17 Scientists. Library Research
Associates Inc, Monroe NY. pp 293-315.

Sara Miller



On Thu, 12 Dec 2002, Jeannette Taylor wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear List , I have a question. How does on go about punching out a disc
} of cultured cells on a plastic culture dish for the purpose of a TEM
} study?
} Without damaging the cells, of course. If possible please give me some
} references so I can read about it. Please reply to my e-mail address,
} below.
} I have been surfing web references but have as yet not found any for
} this specific technique.
}
} thanks, Jeannette Taylor
} jvtaylo-at-emory.edu
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265


From daemon Fri Dec 13 10:16:26 2002



From: Chuck.Butterick-at-degussa.com
Date: Fri, 13 Dec 2002 10:04:31 -0600
Subject: Re: TEM Particle size measurement

Contents Retrieved from Microscopy Listserver Archives
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Ian,

The carbon black industry has developed a particle sizing procedure through
ASTM International (D24 Committee on Carbon Black). The D24 group has
recently rewritten and updated the standard, D3849. The procedure is
copyrighted so no one can/should just give you a copy. However, that
procedure may be of use to you and can be purchased...about $30 on-line I
think. Disclaimer: I am a member of ASTM International and represent my
company's interests at those meetings. I don't receive compensation but
peripherally benefit from the sale of D24 standards because that money does
support some research activities of the D24 group.

What I can tell you is that most of us doing particle sizing (down to 10
nm particles) buy our carbon coated grids from one of several EM supply
companies. That should solve most background problems. Further, if
particle size is what you want, drop your accelerating voltage to increase
contrast so that the particle is as close to black as possible. Then you
can use any number of image analysis programs to threshold out background
gray junk and then identify (e.g. circle in red) all the particulate. We
also deselect any particulate we know is not of interest. Your choice of
magnification is critical. Too low and your particle will have too few
pixels to be relevant. Too high a magnification and you will be taking too
many images and greatly extending analysis time. Dispersion of your
particulate on the grid will also greatly affect the speed of analysis. Too
disperse will cause you again to take lots of images and too concentrated
will cause overlaps.

You have already been given some good advice by others. Hope this helps
too.

Chuck Butterick
Degussa Engineered Carbons



Ian MacLaren
{maclaren-at-tu-dar To: Microscopy {Microscopy-at-sparc5.microscopy.com}
mstadt.de} cc:
Subject: TEM Particle size measurement
12/10/2002 10:22
AM
Please respond
to ian.maclaren






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,
Are other people doing particle size measurement from TEM images, and if
so how? The particles are small metal catalyst particles on carbon
supports. One problem is that automatic image processing could fail
because of the background contrast ripples from the carbon. At the
moment the student is simply measuring everything with a ruler, and this
takes ages, of course.

Secondly, does anyone have a good reference on particle size
distribution? Specifically, we would like to know how distributions are
best represented statistically, particularly when they are non-normal
(short of just showing a diagram for each one).

Best wishes
--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/








From daemon Fri Dec 13 10:57:29 2002



From: Jeannette Taylor :      jvtaylo-at-emory.edu
Date: Fri, 13 Dec 2002 11:47:09 -0500
Subject: technique

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers, thank you all very much for the very helpful suggestions
and leads. I have gotten a request to forward your responses to
another, Silvia Pasquetto, who is also interested in this subject. Does
anyone object?

Jeannette Taylor
jvtaylo-at-emory.edu



From daemon Fri Dec 13 11:24:51 2002



From: Jim Haley :      haley-at-mvia.com
Date: Fri, 13 Dec 2002 12:05:14 -0500
Subject: Re: about coolpix adaptors

Contents Retrieved from Microscopy Listserver Archives
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Michael,

michael shaffer wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} Jim Haley writes ...
}
} } ...
} } The '30 day warranty' [...] is certainly enough time
} } to test the adapter out and verify its quality and
} } determine if the adapter is satisfactory. ...
}
} Before we order, or request a trial, how do we best determine what type of
} 'C-mount' is required. When I looked into this more than a year ago, a 1X
} would have been best for a 9xx Coolpix and its CCD. Is there a formula for
} determining the mag factor of the C-mount adapter relative to the size of
} the CCD in the camera???

If you are going the C-mount route, 1X is always the best to use in
conjunction with our adapters at this point, regardless of camera
model. Bear in mind that in most cases, we can mount to the trinocular
port which eliminates any C-mount concerns and is less expensive. I
usually only recommend going through a C-mount if you have a number of
scopes from different manufacturers and already have 1X C-mount adapters
for them.

} Also ... is the adapter different with regard to older Nikon 9xx and newer
} 5xxx cameras??

The 950, 990, 995 and 4500 require no special adapter rings.
The 5000 does require an adapter ring, but is a straightforward and
works very well.
The 5700 requires both an extender and a thread adapter. I am
recommending at this point that users stay away from this model as it is
prone to EXTREME vignetting with most scopes, and the digital zoom mode
of the camera MUST be used to eliminate the vignetting, which can
degrade image quality.

We do carry all the adapters and spacers mentioned above.

In fact the adapter for the 5000 is being discounted by $20 until
December 31st.

Just in case it isn't obvious:
COMMERCIAL DISCLAIMER: MVIA, Inc. is the supplier of the items discussed
above

Thanks & Happy Holidays!
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
125 Sherwood Drive
Monaca, PA 15061
voice: (724) 728-7493
fax: (412) 291-1709
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

} cheerios ... shAf :o)
} Avalon Peninsula, Newfoundland
} www.micro-investigations.com (in progress)


From daemon Fri Dec 13 12:26:38 2002



From: akc-at-umich.edu
Date: Fri, 13 Dec 2002 13:18:08 -0500
Subject: Re: technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Another approach is to do your cultures in 96-well plates (flat bottom
wells). In this case the wells become acceptable "BEEM capsules," with the
cells in favorable position for sectioning at the tip of the capsule. For
further details of this approach, see Christensen AK and Bourne CM, 1999,
"Shape of large bound polysomes in cultured fibroblasts and thyroid
epithelial cells," Anatomical Record 255:116-129.

--Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Thursday, December 12, 2002 4:25 PM -0500 Jeannette Taylor
{jvtaylo-at-emory.edu} wrote:
}
} Dear List , I have a question. How does on go about punching out a disc
} of cultured cells on a plastic culture dish for the purpose of a TEM
} study?
} Without damaging the cells, of course. If possible please give me some
} references so I can read about it. Please reply to my e-mail address,
} below.
} I have been surfing web references but have as yet not found any for
} this specific technique.
}
} thanks, Jeannette Taylor
} jvtaylo-at-emory.edu
}







From daemon Fri Dec 13 15:25:57 2002



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Fri, 13 Dec 2002 13:13:23 -0800
Subject: Phaser IIsdx transfer rolls

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Does anyone have any 3-color or 4-color transfers for the Phaser IIsdx
dye sublimation printer that they would like to sell? Xerox no longer
has any stocks of color transfer rolls.

I've still a lot of paper to use up and some users who appreciate the
reliability of that printer.

Please no flames about the 'superiority' of ink jets. We recognize the
relative merits of all printer types. This Phaser has been reliable
and reproducible, we intend to run it into the ground as long as
supplies are available.

Thanks in advance,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

************************************************************************
******
The box said "Requires Windows 95 or better", so I bought a Macintosh.
************************************************************************
******



From daemon Fri Dec 13 16:14:30 2002



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 13 Dec 2002 14:04:39 -0800
Subject: Microradiographs?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Happy holidays to everyone, good luck in the new year.

Someone just asked me if I knew anything about getting microradiographs
done. I didn't so, if you do, could you help me out?

The project requires about 200 microradiographs of thin sections from human
femur with a resolution level that will show osteons. They didn't know
exactly what resolution, they guessed in the micrometer range.

We are in N. Calif. so if you know someone who can do this let me know so I
can pass on the contact. They are working on a budget proposal and would
like to get an idea of time and cost.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Dec 13 19:26:00 2002



From: Elaine Humphrey :      ech-at-interchange.ubc.ca
Date: Fri, 13 Dec 2002 16:59:37 -0800
Subject: RE: Cell culture technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
We use a technique for punching out discs of cultured cells for
introduction to the high pressure freezer. This technique works just
as well for conventional fixation.

Grow the cells on membrane inserts in well plates. Use a disposable
biopsy punch to cut out the discs.
We get our plates from Fisher Scientific: 3 micron, clear, transwell
inserts in 6 or 24 well plates. I have one catalogue number of
CS003472
The biopsy punches we get from Dormer Labs - 1.5 mm Acu Punch. They
just fit a high pressure freezer hat or give a good size for
conventional fixation.

The membranes get processed along with the sample and cut with a
diamond knife just fine.

We have also used Aclar film and it works fine too for conventional
fixation. The aclar peels off after polymerizing the resin, leaving
the the cells in the resin, or it can be cut with a diamond. Aclar is
really too thick for high pressure freezer hats, and the transwell
membranes are thin enough.
Elaine


} Jeanette:
}
} I wouldn't punch our a disc from plastic culture dishes. Different plastic
} dishes can partially dissolve depending on the embedding media used.
}
} Tell the researcher that the best way to look at cultured cells for TEM is
} to use ACLAR Embedding Film from EM Sciences. It can be cut to size to fit
} in a petri dish and the cells easily attach. Then you can process the
} Aclar/cells as if normal its a normal tissue sample, embedding it the film
} with the cells face down in a mold. It's a rare cell type that won't grow
} on these sheets, so there should be no problem to plate cells onto the
} ACLAR.
}
} You can buy the ACLAR embedding film from Electron Microscopy Sciences, look
} on page 140 in their catalog.
}
} A second method would be to have the researchers grow cells on thick glass
} coverslips and "pop-off" the cells after embedding and polymerization of the
} epoxy resin. If you want that technique email me directly.
}
} Good Luck!
}
} Karen Bentley
}
} Karen L. Bentley, M.S.(previously Jensen)
} Associate Scientist & Project Manager
} Electron Microscope Research Core
} University of Rochester Medical Center
} Rochester, NY 14642
} 585-275-1954
}
}
} -----Original Message-----
} } From: Jeannette Taylor [mailto:jvtaylo-at-emory.edu]
} Sent: Thursday, December 12, 2002 4:26 PM
} To: Microscopy
} Subject: technique
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Dr. Elaine Humphrey
Director, BioImaging Facility
First Vice President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


From daemon Sat Dec 14 08:18:21 2002



From: Lesley Weston :      lesley-at-vancouverbc.net (by way of
Date: Sat, 14 Dec 2002 08:09:34 -0600
Subject: Re: Cell culture technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I used to process cells in culture dishes for EM. I processed the whole dish
by adding each reagent to the dish, and then after polymerisation I cut out
the required bit with a hacksaw. The dish does start to dissolve, but if you
go straight from anhydrous EtOH to 50/50 EtOH/Epon, without propylene oxide
or any other intermediary, it doesn't cause too much of a problem. Because
you are dealing with a thin layer of cells, you don't need to leave each
reagent in the dish for very long in the resin stages, and that helps too.

Lesley Weston.



on 13/12/2002 7:05 AM, Jensen, Karen at Karen_Jensen-at-urmc.rochester.edu
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Jeanette:
}
} I wouldn't punch our a disc from plastic culture dishes. Different plastic
} dishes can partially dissolve depending on the embedding media used.
}
} Tell the researcher that the best way to look at cultured cells for TEM is
} to use ACLAR Embedding Film from EM Sciences. It can be cut to size to fit
} in a petri dish and the cells easily attach. Then you can process the
} Aclar/cells as if normal its a normal tissue sample, embedding it the film
} with the cells face down in a mold. It's a rare cell type that won't grow
} on these sheets, so there should be no problem to plate cells onto the
} ACLAR.
}
} You can buy the ACLAR embedding film from Electron Microscopy Sciences, look
} on page 140 in their catalog.
}
} A second method would be to have the researchers grow cells on thick glass
} coverslips and "pop-off" the cells after embedding and polymerization of the
} epoxy resin. If you want that technique email me directly.
}
} Good Luck!
}
} Karen Bentley
}
} Karen L. Bentley, M.S.(previously Jensen)
} Associate Scientist & Project Manager
} Electron Microscope Research Core
} University of Rochester Medical Center
} Rochester, NY 14642
} 585-275-1954
}
}
} -----Original Message-----
} } From: Jeannette Taylor [mailto:jvtaylo-at-emory.edu]
} Sent: Thursday, December 12, 2002 4:26 PM
} To: Microscopy
} Subject: technique
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear List , I have a question. How does on go about punching out a disc
} of cultured cells on a plastic culture dish for the purpose of a TEM
} study?
} Without damaging the cells, of course. If possible please give me some
} references so I can read about it. Please reply to my e-mail address,
} below.
} I have been surfing web references but have as yet not found any for
} this specific technique.
}
} thanks, Jeannette Taylor
} jvtaylo-at-emory.edu
}
}


From daemon Sun Dec 15 08:05:28 2002



From: barbara maloney :      maloneyb-at-fiu.edu
Date: Sun, 15 Dec 2002 08:45:00 -0500
Subject: fuchsite?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear group- someone brought me an unidentified artifact supposedly found
in some pyramid that dates to classic Maya, however the style of carving
and the kind of stone used they say is not Mayan. Outside of conducting
an EDS analysis with our SEM - do you have any other suggestions? The
elements that came up was Al, Si, Na, C , K and minimal Mg - does this
suggest any particular type of stone to you?
Thanks
Barb



From daemon Sun Dec 15 13:49:20 2002



From: Gordon Nord :      gnord-at-mindspring.com
Date: Sun, 15 Dec 2002 14:37:57 -0500
Subject: Re: fuchsite?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barbara,

This may be the wrong list for this question.
I suggest Ask-A-Mineralogist at {http://www.minsocam.org} the other and
older MSA, the Mineralogical Society of America.

When dealing with a "stone" - presumably so fine-grained that you can't
see any crystal shapes in a binocular microscope - the important
characteristics will be color, texture, specific gravity and hardness.
Let's assume it is fuchsite. Fuchsite is a green mica and as such is
softer than a pen knife. Jadeite on the other hand, also a Na, Al
silicate is harder than a pen knife. A simple scratch test will
determine the relative hardness.

What color is it?

On Sunday, December 15, 2002, at 08:45 AM, barbara maloney wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear group- someone brought me an unidentified artifact supposedly found
} in some pyramid that dates to classic Maya, however the style of carving
} and the kind of stone used they say is not Mayan. Outside of conducting
} an EDS analysis with our SEM - do you have any other suggestions? The
} elements that came up was Al, Si, Na, C , K and minimal Mg - does this
} suggest any particular type of stone to you?
} Thanks
} Barb
}
}
}


Dr. Gordon Nord
Senior Scientist
Environmental Sciences Laboratory
Brooklyn College
Brooklyn NY 11210



From daemon Mon Dec 16 05:52:32 2002



From: Ken Blight :      Ken.Blight-at-cancer.org.uk
Date: Mon, 16 Dec 2002 11:37:50 +0100
Subject: technique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--
Hi,
I have been reading, with great interest, the replies to
Jeannette Taylor's problem with embedding her cells. I encounter a
similar problem when I try and flat embed cultured cells for
cryo-ultramicrotomy. I have tried to grow Hela cells on both glass
and plastic and then embed in 12% gelatin and infiltrate in 2.3M
sucrose. The only time the cells have come away from the substrate,
successfully, was when one of the samples was fixed heavily for
morphological studies. If the samples are fixed lightly ie 2%
para-formaldehyde, the cells remain firmly adhered to the substrate
even after 2 weeks infiltration in sucrose!
Has anyone had similar problems? Is there a simple answer to
this? Answers on a Christmas card...


Ken Blight
Senior Scientific Officer
Cancer Research UK
London
England


From daemon Mon Dec 16 10:06:07 2002



From: bbauer-at-icc.edu (by way of Ask-A-Microscopist)
Date: Mon, 16 Dec 2002 18:09:56 -0600
Subject: Ask-A-Microscopist: polarized microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barbara -
If you have access to a geologist, he/she may be able to identify the
type of stone for you, and if there are geological maps of the region from
which the artifact came, he/she may be able to identify its provenance.
Probably over half the rocks on the planet contain the elements you list, so
that won't be much help. Not that many types of rock lend themselves to
manual shaping to make things out of them, though, so that could narrow it
down. Soapstone is a famous example from the Canadian Arctic, but there are
lots of others.
Even the various minerals that make up rocks can be tricky to ID with
EDS, since so many of them share typical element compositions. XRD of a bit
of ground-up artifact may identify constituent minerals, but lots of
archaeology types hate having people grind up their stuff, so that may not
be an option.
An interesting problem, though.

F.C. Thomas
FThomas-at-NRCan.gc.ca, (902) 426-4635, facsimile/telecopier (902) 426-6152
GSC Atlantic
Natural Resources Canada/Ressources naturelles Canada
Bedford Institute of Oceanography/ l'Institut Oceanographique du Bedford
P.O. Box 1006/B.P. 1006, Dartmouth, Nova Scotia/Nouvelle Ecosse B2Y 4A2
Government of Canada/Gouvernement du Canada
----- Original Message -----
} From: "barbara maloney" {maloneyb-at-fiu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, December 15, 2002 9:45 AM


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (bbauer-at-icc.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
December 16, 2002 at 10:24:38
---------------------------------------------------------------------------

Email: bbauer-at-icc.edu
Name: Bambi Bauer

Organization: Illinois Central College

Education: Undergraduate College

Location: East Peoria, Illinois, USA

Question: Our college is looking to purchase a comparative, polarized
microscope for our new Criminal Justice Program. Are there
alternatives to the Leica Microsystems, Inc - CFM2 microscope, with
the same quality and features? What does your organization recommend
as possible alternatives? What do you use?

---------------------------------------------------------------------------


From daemon Mon Dec 16 18:19:32 2002



From: rco2-at-ufl.edu (by way of Ask-A-Microscopist)
Date: Mon, 16 Dec 2002 18:11:24 -0600
Subject: Ask-A-Microscopist: GFP transformed bacterial strains &

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (rco2-at-ufl.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
December 16, 2002 at 14:22:18
---------------------------------------------------------------------------

Email: rco2-at-ufl.edu
Name: Robin Oliver

Organization: University of Florida

Education: Graduate College

Location: Gainesville, FL, USA

Question: I am currently trying to look at two GFP transformed
bacterial strains in the xylem of plant tissue with a confocal
microscope. I started out making think hand sections but the lamp (i
think) was so hot, it started pulling water out of the sample. This
not only made bubbles under my sample but also made it impossible to
increase the magnification.

Then, I tried to get rid of the water in my samples by drying them
out overnight. This made the autofluorescence (yellow) of the tissue
so intense, it was virtually impossible to see my two bacterial
strains (yellow and blue).

So, how can I either get rid of the water in my sample or quench the
autofluorescence of my plant tissue. There is some debate at this
point as to if using a freezing microtome to cut my samples would
decrease the water as well as the autofluorescence. Finally, should
I use a special mounting medium, would this help these problems?

Any help anyone could give would be greatly appreciated!!!

R. Oliver

---------------------------------------------------------------------------


From daemon Mon Dec 16 18:19:33 2002



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu (by way of
Date: Mon, 16 Dec 2002 18:09:07 -0600
Subject: Evaporator cold trap

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to put a LN2 cold trap
on it, in an attempt to clean up the vacuum a tad more. There is a 3.5 inch
by 4.5 inch plate right above the diffusion stack which looks to be a great
place to attach a cold trap. Rumor has it that this was an option. Are there
any old traps kicking around that one can acquire? I could have our machine
shop manufacture one, but was hoping for a cheaper route.
Thanks in advance,
Randy Nessler
319-335-8142


From daemon Mon Dec 16 18:20:31 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 16 Dec 2002 16:15:56 -0800
Subject: TMV for TEM standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,
Does anyone know where to obtain tobacco mosaic virus to use as an
internal calibration standard? TIA for your help.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Mon Dec 16 22:57:11 2002



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Tue, 17 Dec 2002 14:51:43 +1000
Subject: Zeiss Diffraction Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day Guys,
Some years ago Zeiss produced a kit for demonstrating diffraction and
aberrations in the optical microscope. I recently obtained one of these
kits, it seems almost complete, but I require a copy of the instructions
for its use. If I remember correctly it was a small booklet in German.
If anyone has a copy or even better an english translation or set of
instructions would it be possible to get a copy.
Thanks
Merry Christmas and Happy New Year to all

Regards

John V Nailon
Executive Officer and Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld



From daemon Tue Dec 17 09:15:59 2002



From: George_Munzing-at-engelhard.com
Date: Tue, 17 Dec 2002 09:57:56 -0500
Subject: RE: Gatan environmental holder availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings all.

I have come across a Gatan model 628/2 single tilt hot stage (1300C) and
heating control unit that was purchased for a Hitachi H600 100Kv TEM but
was never used. It was apparently tucked away in the lab by my predecessor
immediately after its purchase and was recently discovered after having
already replaced this microscope. I would like to know if anyone with a
similar microscope is interested in actually using it.

Please contact me off-line to discuss in more detail.

George R. Munzing Jr.
Engelhard Corporation
25 Middlesex-Essex Tpk.
Iselin, NJ 08830
TELE 732-205-7030
FAX 732-205-5300




From daemon Tue Dec 17 09:25:27 2002



From: michael shaffer :      michael-at-shaffer.net
Date: Tue, 17 Dec 2002 11:48:00 -0330
Subject: RE: Evaporator cold trap

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy writes ...

} I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to
} put a LN2 cold trap on it, in an attempt to clean up the
} vacuum a tad more. ...

Just to point out (... in case someone has one of these laying around
..), the trap doesn't necessarily have to be LN type. A cold water chevron
trap will also work very effectively.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)




From daemon Tue Dec 17 09:42:09 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 17 Dec 2002 08:27:27 -0700
Subject: Ask-A-Microscopist: polarized microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suggest you check out McCrone in Chicago. They do a lot of forensics and
polarized microscopy, and they sell those, too. Here is a URL to start:

http://www.mccrone.com/cgi-bin/SoftCart.exe/mac/microscopes/index.html?E+mcc
rone

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: bbauer-at-icc.edu [mailto:bbauer-at-icc.edu]
Sent: Monday, December 16, 2002 5:10 PM
To: Microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (bbauer-at-icc.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
December 16, 2002 at 10:24:38
---------------------------------------------------------------------------

Email: bbauer-at-icc.edu
Name: Bambi Bauer

Organization: Illinois Central College

Education: Undergraduate College

Location: East Peoria, Illinois, USA

Question: Our college is looking to purchase a comparative, polarized
microscope for our new Criminal Justice Program. Are there
alternatives to the Leica Microsystems, Inc - CFM2 microscope, with
the same quality and features? What does your organization recommend
as possible alternatives? What do you use?

---------------------------------------------------------------------------


From daemon Tue Dec 17 10:23:42 2002



From: saram-at-duke.edu
Date: Tue, 17 Dec 2002 11:05:49 -0500 (EST)
Subject: Re: Ask-A-Microscopist: GFP transformed bacterial strains & fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


See CAPS interspersed below.

On Mon, 16 Dec 2002, by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (rco2-at-ufl.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} December 16, 2002 at 14:22:18
} ---------------------------------------------------------------------------
}
} Email: rco2-at-ufl.edu
} Name: Robin Oliver
}
} Organization: University of Florida
}
} Education: Graduate College
}
} Location: Gainesville, FL, USA
}
} Question: I am currently trying to look at two GFP transformed
} bacterial strains in the xylem of plant tissue with a confocal
} microscope. I started out making think hand sections but the lamp (i
} think) was so hot, it started pulling water out of the sample. This
} not only made bubbles under my sample but also made it impossible to
} increase the magnification.
}
WHY DON'T YOU USE A COVERSLIP AND SEAL THE EDGES TO KEEP IN THE WATER?

} Then, I tried to get rid of the water in my samples by drying them
} out overnight. This made the autofluorescence (yellow) of the tissue
} so intense, it was virtually impossible to see my two bacterial
} strains (yellow and blue).
}
IS YOUR TISSUE FIXED IN GLUTARALDEHYDE? GLUT IS AUTOFLU0RESCENT.

YOU CAN TRY QUENCHING THE AUTOFLUORESCENCE WITH SOMETHING LIKE GLYCINE OR
AMMONIUM CHLORIDE. I'M NOT SURE THIS WILL WORK, BUT YOU CAN TRY IT.

} So, how can I either get rid of the water in my sample or quench the
} autofluorescence of my plant tissue. There is some debate at this
} point as to if using a freezing microtome to cut my samples would
} decrease the water as well as the autofluorescence. Finally, should
} I use a special mounting medium, would this help these problems?
}
SOME MOUNTING MEDIA CONTAIN GLYCEROL AND GLYCINE. IF VIEWING THE TISSUE
WET WORKS, START WITH JUST KEEPING IT WET BY SEALING IT (E.G., WITH
FINGERNAIL POLISH AROUND THE EDGES). THEN, IF THAT DOESN'T DO IT, TRY THE
GLYCEROL/GLYCINE.

IF YOU NEED FLATTER SECTIONS, TRY A TISSUE SLICER. SEVERAL EM SUPPLIERS
CARRY THEM.

} Any help anyone could give would be greatly appreciated!!!
}
} R. Oliver
}
GOOD LUCK,
SARA MILLER
} ---------------------------------------------------------------------------
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265


From daemon Tue Dec 17 10:35:01 2002



From: John Shields :      jshields-at-cb.uga.edu
Date: Tue, 17 Dec 2002 11:27:52 -0500
Subject: potassium dichromate treatment

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Hello,
I received cells that have been sitting in potassium dichromate
and the researchers requested that I do propane jet freezing.
I don't have any experience with cells that are treated in this
manner before processing for TEM.
I would appreciate hearing from anyone have comments on the
eventual quality of fixation (of any sort) or other concerns.
Thanks
John Shields
EM Lab
Univ. of Georgia



From daemon Tue Dec 17 11:32:40 2002



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Tue, 17 Dec 2002 09:19:04 -0800
Subject: Re: Ask-A-Microscopist: polarized microscope

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I'm a Firearm and Toolmark examiner so do not work with polarized light comparison scopes but do work with comparison scopes in a forensic lab. When I worked at ISP lab Morton I occasionally lectured at the Criminalistics overview course that ICC had at that time, one or two lectures a semester as a guest of the instructor. Here are some leads that might help.

Leeds http://www.leedsmicro.com/ offers basically customized comparison microscopes, their hair and fiber scopes are based upon Olympus Scope bodies, they would gladly use pol. scopes for the extra cost I'm sure. When I talked to them about a firearms scope they indicated they basically custom built each scope for a customer but for their firearms scope they use a zoom lens system and I do not like zoom lens systems. Our lab just bought one of their hair comparison scopes and our hair and fiber people like it quite a bit. They didn't get the pol. scope as they do their pol. light mic. on the single pol. scope then go to the comparison scope for side by side examination.

Leica http://www.leica.com/index.html offers the UFM-4 which is designed for firearms and Toolmark work, they offer a hair and fiber scope (I don't know the model #) as well but neither is a pol. scope as such but they may offer something that I haven't seen. They may well make accessories available that would let you convert their scope. I've added sub-stage polarizing filters for work comparing fingernails in the past to the UFM-4 and as indicated the UFM-4 is not designed for the type work you would be doing with a polarized light comparison scope.

Most forensic labs don't use a polarized comparison scopes except occasionally. It use to be very difficult to get good light balance and you didn't want to add polarizers to the problem. The newer scopes have good light balance that can be more easily dealt with. With the new microscopes and their easily balanced lighting systems many more labs may go that way. You are looking at adding expensive stages and strain free optics so quite a bit more money, a few thousand more, on an already expensive microscope.

You may want to get together with Ill. State Police Lab Morton (309-284-6500) which is very close to you and talk to them about comparison scope use. I don't know if they have anyone on staff there that is doing hair or fiber work right now (staff has changed a great deal in the 13 years I've been gone). But they could put you together by phone with one of the people that teach the work in their training lab if not. I'm sure that the lab staff would still be glad to help if asked. Before you spend around 40 or 50K I'd talk to as many people as you can.


Jim



James L. Roberts
Firearm & Toolmark Examiner
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 654-2308

James.Roberts-at-mail.co.ventura.ca.us


} } } {bbauer-at-icc.edu} 12/16/02 04:09PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (bbauer-at-icc.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
December 16, 2002 at 10:24:38
---------------------------------------------------------------------------

Email: bbauer-at-icc.edu
Name: Bambi Bauer

Organization: Illinois Central College

Education: Undergraduate College

Location: East Peoria, Illinois, USA

Question: Our college is looking to purchase a comparative, polarized
microscope for our new Criminal Justice Program. Are there
alternatives to the Leica Microsystems, Inc - CFM2 microscope, with
the same quality and features? What does your organization recommend
as possible alternatives? What do you use?

---------------------------------------------------------------------------





From daemon Tue Dec 17 11:54:20 2002



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Tue, 17 Dec 2002 12:58:21 -0500
Subject: RE: Cold traps & Baffles

Contents Retrieved from Microscopy Listserver Archives
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You should be aware that installing a cold trap or baffle between the
diffusion pump and vacuum chambew will decrease the pumping speed of
the system significantly - usually by about a factor of two. This
will, in turn, increase the time required to pump down to an
operating vacuum. You need to take this effect into consideration
before adding such a device to your evaporator. The pumnpdown
process is discussed in some detail in Chapter 2 of my book 'Vacuum
Methods in Electron Microscopy' (for a description see:
http://www.2spi.com/catalog/books/book48.html) and the
characteristics of traps and baffles are discussed in Chapter 5.
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Tue Dec 17 15:06:47 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 17 Dec 2002 15:56:11 -0500
Subject: TMV for TEM standard

Contents Retrieved from Microscopy Listserver Archives
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OK Bill here goes,
I wanted to know too.
http://www.asu.edu/clas/csss/SPM/IG_gifs/asutmv2.html
http://masspec.scripps.edu/publications/pdf/2001_AngewChem.pdf
and finally the source of sources,
http://www.atcc.org/SearchCatalogs/PlantVirology.cfm (in the event
that the next is broken)

http://www.atcc.org/SearchCatalogs/longview.cfm?view=plvrs,33422,PV-135P&tex
t=tmv&max=20

I thought I remembered that the ATTC had the stuff.

Regards to you both,

A Merry Xmas and Happy New Year to all,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone: 610-738-0437
eMail: fmonson-at-wcupa.edu
An FEI (Quanta 400 and Technai 12),
Oxford INCA Energy 400, and
Olympus FV-300 Shop.


-----Original Message-----
} From: Bill Tivol [mailto:tivol-at-caltech.edu]
Sent: Monday, December 16, 2002 7:16 PM
To: microscopy-at-sparc5.microscopy.com


Dear List,
Does anyone know where to obtain tobacco mosaic virus to use as an
internal calibration standard? TIA for your help.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Wed Dec 18 03:31:01 2002



From: Saif Amer :      saifa-at-squ.edu.om
Date: Wed, 18 Dec 2002 13:22:19 +0400
Subject: SEM Course

Contents Retrieved from Microscopy Listserver Archives
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Hi
I am a technician in earth science department in sultan Qaboos University i
am working in SEM lab, I have JOEL 840A JSM with link Isis 300, I am looking
for training in in this machine but unfortunately I did not find good one
for me yet, . No I am trying again hope get chance this time. If any one can
help me in this.
thanks

saif

Saif Amer Al Mammari
Sultant of Oman, Muscat
Sultan Qaboos University
College of Science
Earth Science Department



From daemon Wed Dec 18 09:26:29 2002



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Wed, 18 Dec 2002 10:13:53 -0500
Subject: RE: LM: CCD camera for LM

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John:

We don't have the D100, but we do have the Kodak 760. The Kodak 760,
along with the Nikon D1 and D1X all based on very similar professional level
true SLR bodies with the Nikkor AF / F-mount, D-Type lens mount and
autofocusing system. The 760, D1, DH, D1x are based on the professional
F5 body, the D100 is based on the F80 body (albeit modified). The D100 is
matched against the Canon EOS-D60 in general performance.

As far as mounting any of these cameras on a microscope goes its very
easy, if you have a scope with a trinocular head just treat these cameras like
a normal 35mm film camera. Use a standard F-mount or F-mount adapter
from the microscope vendor. You will need to adjust from the standard
photoeye piece lens. Since the (Sony) CCD sensor is 23.7 x 15.6 mm or
1.8" / diagonal 28.4 mm (APS sized) its a little smaller than the standard
35mm format. The APS C-type film format is 23.4 x 16.7mm, and 35mm film
format is 35.0 x 23.3 - therefore there is a 1.5x focal multiplier factor
difference between the D100 sensor and 35mm film so you will need a lower
mag photoeye piece if you want the whole field and not the "sweet spot".
However all of these cameras are designed to (1) work with standard film
format lenses, and (2) be SLR so you see what you get.

(Side note: "The EOS-1Ds is Canon's newest professional SLR. Based on the
EOS-1D body the EOS-1Ds raises resolution to 11 megapixels, uses a CMOS
sensor (just like the EOS-D30 and D60) and is the first Canon digital SLR with
a sensor which captures a full 35 mm frame. " --- Dec. 17, 2002)

A Draw back for microscopy work is a fixed viewfinder prism and
focusing screen. I know with the Kodak 760, the first thing I did was replace
the focusing screen (Nikon M and C Focusing Screens work well) and got the
Nikon DW-31 High Mag (6x) Finder (Right angle finder so you don't have to
climb up on top of the scope to look through the camera view finder.

For a solid photographic review of the camera go and see:
http://www.dpreview.com/ (they review digital cameras) They also included a
series of full resolution images from each camera reviewed as well as user
reviews.



} List:
} Does anyone have experience with Nikons new low end professional digital SLR
} offering, model D100? It uses a standard Nikon SLR body and mount so most of
} their lenses fit and auto features will work with many of the lenses (AF & D
} type that we use). It is a 6 mega pixel camera listing for ~$2,000 (body only).
} Since we currently use Nikon SLR camera's on our OM systems and have several
} Nikon lenses for copy stand work this seems like a very good fit. Considering
} all of the costs associated with purchasing and mounting fixed lens consumer
} camera's onto microscopes it appears to be a viable option? True the D100 costs
} are slightly higher however the benefits are significant, high resolution
} images, selectable lens ranges, improved lens quality, versatile camera
} software, and I am assuming it uses standard microscope mounting hardware found
} in many labs.
}
} What have I missed? Are there pitfalls associated with this camera? Since this
} is a newer camera model I can not confirm some of the microscope mounting
} information. Please correct any errors or misinformation above. Any additional
} input or insight would be appreciated.
}
} Sincerely, jr
}
}
}
} John Robson
} Boehringer Ingelheim Pharmaceuticals, Inc.
} PO Box 368
} 900 Ridgebury Rd
} Ridgefield, CT 06877
}
} Phone (203)798-5640
} Fax (203)798-5698
}
} e-mail jrobson-at-RDG.boehringer-ingelheim.com
}
}
} -----Original Message-----
} } From: "Mortro-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Mortro-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Tuesday, December 10, 2002 11:08 AM
} To: haley-at-mvia.com; atcsem-at-earthlink.net
} Cc: jfactor-at-purvid.ns.purchase.edu; Microscopy-at-sparc5.microscopy.com;
} atcsem-at-earthlink.net
} Subject: Re: LM: CCD camera for LM
}
} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'd recommend MVIA's Coolpix adapters. We have been using them in the lab
} for a few months now.
}
} We had tried unsuccessfully to get good images using our Coolpix 995 camera with
} an adapter from a different vendor. This summer we decided to try the adapters
} from MVIA. The adapters have worked great for us and we can now get good images
} from the camera.
}

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Wed Dec 18 13:54:35 2002



From: Tom Budd :      tbudd-at-stlawu.edu
Date: Wed, 18 Dec 2002 14:33:01 -0500
Subject: Re: sorval

Contents Retrieved from Microscopy Listserver Archives
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Tom Budd wrote:

} listers
}
} i have an old sorvall MT-1 ultramicrotome that i would like to put back into
} service. i am lacking the base stand that had the "boom" for holding the
} dissecting microscope as well as the fluorescent light fixture. if any of you
} have these in your storage closet, i would be glad to take them off your hands
} and free up some storage space for you. of course, i would also pay for
} shipping and/or a modest fee for the items.
}
} please respond off-list if you can help me.
}
} thanks and happy holidays
}
} tbudd
} --
} Dr. T. Budd
} Chair of Biology
} St. Lawrence University
} Canton, NY 13617
} Phone = 315-229-5640
} Fax = 315-229-7429
} E-mail = tbudd-at-stlawu.edu
}
} This message is made of 100% recycled electrons!

--
Dr. T. Budd
Chair of Biology
St. Lawrence University
Canton, NY 13617
Phone = 315-229-5640
Fax = 315-229-7429
E-mail = tbudd-at-stlawu.edu

This message is made of 100% recycled electrons!




From daemon Thu Dec 19 09:21:19 2002



From: Mucciolo Antonio :      antonio.mucciolo-at-eivd.ch (by way of
Date: Thu, 19 Dec 2002 09:09:23 -0600
Subject: SEM: removing paraffine around a cut from a biopsy

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir,

may I take a few moments of your precious time? I should like to have access
to two types of information you are likely to provide.

1) How to remove the paraffine around a cut from a biopsy in which the
presence of silicone is
expected? (our main concern is to avoid the migration of said silicone
during the removal of the paraffine)

2) How to best perform a biopsy of a tissue containing silicone destined for
study in a SEM? (we are especially looking for details regarding cutting
and holding in place the silicone)

Thank you very much in advance,
Yours respectfully,

---------------------------------------------------------------------------

Email: antonio.mucciolo-at-chuv.hospvd.ch
Name: Mucciolo

Organization: CHUV(hospital)

Education: Graduate College

Location: lausanne, suisse

Question: how to prepare fabrics human with implants silicone for the
analysis in electronic microscopy

---------------------------------------------------------------------------


From daemon Thu Dec 19 17:01:57 2002



From: Robert Kayton :      kayton-at-ohsu.edu
Date: Thu, 19 Dec 2002 14:20:30 -0800
Subject: Cleaning a Penning Gauge

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I need some advice on cleaning a penning gauge. I have an Edwards Model CP 25-K, on a Cambridge 360 Stereoscan. There are some very stubborn deposits, especially on the three inserts. Any suggestions are appreciated

thanks.

Bob Kayton, PhD
Histo/EM Core
503-494-2504-Lab
503-703-3938-Cell



From daemon Fri Dec 20 02:26:49 2002



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 20 Dec 2002 08:13:51 +0000 (GMT Standard Time)
Subject: Re: Cleaning a Penning Gauge

Contents Retrieved from Microscopy Listserver Archives
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Hi Robert,

Both the cups (Cathode cups, part number D145-33-007) and
post (Anode, part number D145-33-006) can be replaced. They
can also be cleaned by abrasion (SiC paper) and reused but
beware that the anode has been surface treated and will not
last very long after abrasion (about 6 months) so it is
better to replace it. Parts from your local Edwards agent.

I use SiC paper and wash with alcohol afterwards for the
cathodes and the body of the gauge, I only replace the
cathodes when they are really shot. Note the orientation of
the cathode cups when you take them out.

Good luck,
Ron

On Thu, 19 Dec 2002 14:20:30 -0800 Robert Kayton
{kayton-at-ohsu.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I need some advice on cleaning a penning gauge. I have an Edwards Model CP 25-K, on a Cambridge 360 Stereoscan. There are some very stubborn deposits, especially on the three inserts. Any suggestions are appreciated
}
} thanks.
}
} Bob Kayton, PhD
} Histo/EM Core
} 503-494-2504-Lab
} 503-703-3938-Cell
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk
http://www-em.materials.ox.ac.uk/
*********************************

We are pleased to host EMAG'03 in Oxford. 3rd to 5th September 2003.

********************************



From daemon Fri Dec 20 07:09:38 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Fri, 20 Dec 2002 07:58:14 -0500
Subject: Re: Cleaning a Penning Gauge

Contents Retrieved from Microscopy Listserver Archives
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Robert,
If you have access to a bead blaster (sand blaster that uses fine glass
beads), it's a lot less work than SiC. Any aluminum parts should be
done with a reduced pressure of 35-40 psi or you may find that the
aluminum disappears pretty quickly. If the stainless steel parts don't
clean up at that pressure, you can go back up to 100 psi or so. Be sure
to mask any sealing surfaces with masking tape before blasting. I don't
believe there are any screw threads inside your gauge, but some have
threaded parts. Male threads should be masked with masking tape and
female threads should have a properly sized machine screw inserted to
protect the threads.

Ken Converse
owner
Quality Images
Third party SEM service
Delta, PA

Robert Kayton wrote:

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From daemon Fri Dec 20 08:59:36 2002



From: bai xuedong :      xdbai-at-aphy.iphy.ac.cn
Date: Fri, 20 Dec 2002 22:33:54 +0800 (CST)
Subject: used specimen holder of JEOL 2010F

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Hello,
I need a used specimen holder for Jeol 2010F TEM. Do you have any
information? Thanks
*************************************************************
Xuedong Bai
School of Materials Science and Engineering
Georgia Institute of Technology
771 Ferst Drive, N. W.
Atlanta, GA 30332-0245
Phone: (404)385-0326 (O); (404)875-2099 (H)
Fax: (404)894-9140
Email: xb8-at-mail.gatech.edu
**************************************************************


From daemon Fri Dec 20 09:43:19 2002



From: John Skvarla :      jskvarla-at-ou.edu
Date: Fri, 20 Dec 2002 09:37:03 -0600
Subject: Carbon tape reference

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Does anyone have the original reference citation to the use of
double-stick carbon tape on SEM specimen holders?

Thanks in advance.

John Skvarla


From daemon Fri Dec 20 14:44:05 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 20 Dec 2002 12:41:49 -0800
Subject: Re: Cleaning a Penning Gauge

Contents Retrieved from Microscopy Listserver Archives
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I killed the anode on my Varian gauge by sanding it. Since that was
happening, I am very careful with anodes. I would suggest to replace it if
possible or try mild organic solvent to remove the build up. It looks like
any mechanical treatment is not good for anodes. Sergey

At 08:13 AM 12/20/02 +0000, you wrote:
} ------------------------------------------------------------------------
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------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Fri Dec 20 14:59:20 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 20 Dec 2002 13:00:32 -0800
Subject: Re: Evaporator cold trap

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Randy
I am sorry for late comments. I agree with Wil Bigelow that LN2 trap will
reduce the pump performance. If you need just to increase the system's
overall performance, I would suggest you have to do very good service for
it first (replace all suspicious O-rings, clean everything up). I highly
recommend to use Santovac-5 DP (I assume, it's DP based system, because TP
don't need LN2). When I come in UCLA, I had DV-502A vacuum system with
5*10-5 torr. I cleaned up DP (messy work and a lot of solvents), replaced
all O-rings (the system was 10 y.o. at the moment) and used
Santovac-5. Since that I do have 5*10-7 with no LN2 (and no further
service for more than 5 years). As an alternative, you may install some
"cold finger" with protective screens near your sample in the Bell
Jar. You really need LN2 trap over DP if you pump a lot of water in your
experiments. Sergey

At 06:09 PM 12/16/02 -0600, you wrote:
} ------------------------------------------------------------------------
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------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Fri Dec 20 15:11:04 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 20 Dec 2002 13:12:13 -0800
Subject: Re: technique

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Ken, hello

If the cells are already fixed, you could scrub them from the surface and
then concentrate by centrifugation. Surprisingly (to me) such terrible
procedure does not affect the structure. Another way to do it
"scientifically" - you may use tripsin to detach cells from the
surface. It's the standard procedure in the cell-biology. They do have
pre-made tripsin solution, you just substitute cultural media on tripsin
solution for couple of minutes, discard tripsin and then resuspend cells in
the fresh media. Sergey

At 11:37 AM 12/16/02 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Fri Dec 20 17:44:57 2002



From: charles j day :      wa5ekh-at-juno.com (by way of MicroscopyListserver)
Date: Fri, 20 Dec 2002 17:34:11 -0600
Subject: Fw: Voyager II Users?

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Holiday Greetings!!

I've inherited an old Voyager II (12 years old??) Sun System, I think.
Looking for any remaining active users, list servers, user groups, and
latent programers, extra parts, software, printers, accessories, etc. to
support my future use of this system.
Jeff/Texas
wa5ekh-at-juno.com


From daemon Fri Dec 20 18:06:29 2002



From: saram-at-duke.edu
Date: Fri, 20 Dec 2002 18:55:40 -0500 (EST)
Subject: Re: technique

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Trypsinized cells round up and change morphology. If you're interested in
morphology, you don't want to introduce a variable other than your
experimental.

Also, I wouldn't soak cells for long times in sucrose. Aldehyde fixatives
are somewhat reversible, especially formaldehyde. Lightly fixed cells
sitting in aqueous solutions for a long time will change shape too.
Infiltrate cells for ultracryotomy with 2.1M sucrose in PBS for 20-30 min
and then freeze.

Sergey is right, you can fix the cells for a few minutes (10), scrape them
up with a cell scraper, rubber policeman, or other scraper, pellet them,
further fix them as a pellet (which helps them stick together) and then
process them as a block. It might be necessary to encase the block in
molten agar cooled to about 40 degrees to keep them together. Just don't
fix the agar in glut or it will prevent further infiltration of other
solutions.

For conventional microtomy, cells can be grown on films and the films
sectioned or embedded in situ in dishes or culture slides and peeled up
as a slab, as described here recently.

Season's greetings,
Sara Miller


On Fri, 20 Dec 2002, Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Ken, hello
}
} If the cells are already fixed, you could scrub them from the surface and
} then concentrate by centrifugation. Surprisingly (to me) such terrible
} procedure does not affect the structure. Another way to do it
} "scientifically" - you may use tripsin to detach cells from the
} surface. It's the standard procedure in the cell-biology. They do have
} pre-made tripsin solution, you just substitute cultural media on tripsin
} solution for couple of minutes, discard tripsin and then resuspend cells in
} the fresh media. Sergey
}
} At 11:37 AM 12/16/02 +0100, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } -----------------------------------------------------------------------.
} }
} }
} }
} } --
} } Hi,
} } I have been reading, with great interest, the replies to
} } Jeannette Taylor's problem with embedding her cells. I encounter a
} } similar problem when I try and flat embed cultured cells for
} } cryo-ultramicrotomy. I have tried to grow Hela cells on both glass and
} } plastic and then embed in 12% gelatin and infiltrate in 2.3M sucrose. The
} } only time the cells have come away from the substrate, successfully, was
} } when one of the samples was fixed heavily for morphological studies. If
} } the samples are fixed lightly ie 2% para-formaldehyde, the cells remain
} } firmly adhered to the substrate even after 2 weeks infiltration in sucrose!
} } Has anyone had similar problems? Is there a simple answer to
} } this? Answers on a Christmas card...
} }
} }
} } Ken Blight
} } Senior Scientific Officer
} } Cancer Research UK
} } London
} } England
}
} ------------------------------------------------------
}
} Sergey Ryazantsev, Ph.D.
} Electron Microscopy
} Department of Biological Chemistry, School of Medicine
} University of California, Los Angeles
} Box 951737
} Los Angeles, CA 90095-1737
}
} (310) 825-1144 (office)
} Pager: (310) 845-0248
} FAX: (310) 206-5272 (departmental)
} mailto:sryazant-at-ucla.edu
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265


From daemon Fri Dec 20 20:23:27 2002



From: Allen Sampson :      ars-at-sem.com
Date: Fri, 20 Dec 2002 20:13:17 -0600
Subject: RE: Evaporator cold trap

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Sergey,

Have you verified that vacuum level with an independent, calibrated vacuum
gauge? Vacuum sounds way to high for that system. I agree with all of
your recommendations, but case in point, the vacuum you claim is on an
order of what we normally find in a valve isolated electron gun (much
smaller vacuum and seal volumes) pumped by a 20 L/sec ion pump. The vacuum
level you stated for the start of your work is far closer to the best I
have seen out of this particular evaporator after a complete rebuild.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Friday, December 20, 2002 3:01 PM, Sergey Ryazantsev
[SMTP:sryazant-at-ucla.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Randy
} I am sorry for late comments. I agree with Wil Bigelow that LN2 trap will
} reduce the pump performance. If you need just to increase the system's
} overall performance, I would suggest you have to do very good service for
} it first (replace all suspicious O-rings, clean everything up). I highly
} recommend to use Santovac-5 DP (I assume, it's DP based system, because
TP
} don't need LN2). When I come in UCLA, I had DV-502A vacuum system with
} 5*10-5 torr. I cleaned up DP (messy work and a lot of solvents),
replaced
} all O-rings (the system was 10 y.o. at the moment) and used
} Santovac-5. Since that I do have 5*10-7 with no LN2 (and no further
} service for more than 5 years). As an alternative, you may install some
} "cold finger" with protective screens near your sample in the Bell
} Jar. You really need LN2 trap over DP if you pump a lot of water in your
} experiments. Sergey
}
} At 06:09 PM 12/16/02 -0600, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi All,
} } I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to put a LN2
cold
} } trap
} } on it, in an attempt to clean up the vacuum a tad more. There is a 3.5
inch
} } by 4.5 inch plate right above the diffusion stack which looks to be a
great
} } place to attach a cold trap. Rumor has it that this was an option. Are
there
} } any old traps kicking around that one can acquire? I could have our
machine
} } shop manufacture one, but was hoping for a cheaper route.
} } Thanks in advance,
} } Randy Nessler
} } 319-335-8142
}
} ------------------------------------------------------
}
} Sergey Ryazantsev, Ph.D.
} Electron Microscopy
} Department of Biological Chemistry, School of Medicine
} University of California, Los Angeles
} Box 951737
} Los Angeles, CA 90095-1737
}
} (310) 825-1144 (office)
} Pager: (310) 845-0248
} FAX: (310) 206-5272 (departmental)
} mailto:sryazant-at-ucla.edu
}
}



From daemon Fri Dec 20 20:42:08 2002



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 20 Dec 2002 18:36:39 -0800
Subject: Re: Cleaning a Penning Gauge

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On Friday, December 20, 2002, at 04:58 AM, qualityimages wrote:

} If you have access to a bead blaster (sand blaster that uses fine
} glass beads), it's a lot less work than SiC. Any aluminum parts
} should be done with a reduced pressure of 35-40 psi or you may find
} that the aluminum disappears pretty quickly.

} Ken Converse
}
Dear Ken and Robert,
The instrument I used, called an "air eraser", offered a choice of
glass beads, corundum, or, I think, starch grains (in any case,
something pretty soft) to use as the abrading particles. The soft
abraders could be useful for Al; however, the dirt may be tougher than
the Al, making the process unsuitable. The air eraser is ~$100, and
it's useful for a number of tasks.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



From daemon Sat Dec 21 08:36:19 2002



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Sat, 21 Dec 2002 10:23:23 -0000
Subject: Cleaning Penning Gauge

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to: Bob Kayton;

I'm don't know what the electrodes of a Penning gauge are made from,
neither have I needed to try this on ours. But one reagent that is
good for removing carbon or similar deposits is a solution of, say,
10% potassium hydroxide in ethanol or methylated spirits. Simply soak
for half and hour, rinse with distilled water, and if necessary rub
off with a very soft cloth.

However, this reagent goes like mad for aluminium (and makes some
beautiful etch pits). But if all other liquid reagents fail, you
might try this.

+-----------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+-----------------------------------------+
Phone:
{direct line +44 (0) 118 9318572
{University internal extension 7867
Fax: +44 (0) 118 9750203
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+-----------------------------------------+


From daemon Sat Dec 21 09:17:21 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Sat, 21 Dec 2002 10:09:02 -0500
Subject: Re: Cleaning a Penning Gauge

Contents Retrieved from Microscopy Listserver Archives
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Sergy,
These are the ones I clean most often. Yes, the anodes must be treated
carefully, as they are aluminum, but they also are subject to ion
etching and will eventually "go away" just from use. I've found that
when the central spindle loses about 1/3 of its diameter, they don't
work so well any more and a new anode should be installed. If you have
access to a machine shop, they're not too complicated to make. Last
time I looked Varian wanted $180 for a cleaning kit that contained 1
spare anode and that was many years ago. Custom should be much cheaper.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I killed the anode on my Varian gauge by sanding it. Since that was
} happening, I am very careful with anodes. I would suggest to replace
} it if possible or try mild organic solvent to remove the build up. It
} looks like any mechanical treatment is not good for anodes. Sergey
}
} At 08:13 AM 12/20/02 +0000, you wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi Robert,
} }
} } Both the cups (Cathode cups, part number D145-33-007) and
} } post (Anode, part number D145-33-006) can be replaced. They
} } can also be cleaned by abrasion (SiC paper) and reused but
} } beware that the anode has been surface treated and will not
} } last very long after abrasion (about 6 months) so it is
} } better to replace it. Parts from your local Edwards agent.
} }
} } I use SiC paper and wash with alcohol afterwards for the
} } cathodes and the body of the gauge, I only replace the
} } cathodes when they are really shot. Note the orientation of
} } the cathode cups when you take them out.
} }
} } Good luck,
} } Ron
} }
} } On Thu, 19 Dec 2002 14:20:30 -0800 Robert Kayton
} } {kayton-at-ohsu.edu} wrote:
} }
} } }
} } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } -----------------------------------------------------------------------.
} } }
} } }
} } } I need some advice on cleaning a penning gauge. I have an Edwards
} } Model CP 25-K, on a Cambridge 360 Stereoscan. There are some very
} } stubborn deposits, especially on the three inserts. Any suggestions
} } are appreciated
} } }
} } } thanks.
} } }
} } } Bob Kayton, PhD
} } } Histo/EM Core
} } } 503-494-2504-Lab
} } } 503-703-3938-Cell
} } }
} } }
} } }
} }
} } ----------------------
} } Mr. R.C. Doole
} } Department of Materials,
} } University of Oxford.
} } Parks Road, Oxford. OX1 3PH. UK.
} } Phone +44 (0) 1865 273701
} } Fax +44 (0) 1865 283333
} } ron.doole-at-materials.ox.ac.uk
} } http://www-em.materials.ox.ac.uk/
} } *********************************
} }
} } We are pleased to host EMAG'03 in Oxford. 3rd to 5th September 2003.
} }
} } ********************************
}
}
} ------------------------------------------------------
}
} Sergey Ryazantsev, Ph.D.
} Electron Microscopy
} Department of Biological Chemistry, School of Medicine
} University of California, Los Angeles
} Box 951737
} Los Angeles, CA 90095-1737
}
} (310) 825-1144 (office)
} Pager: (310) 845-0248
} FAX: (310) 206-5272 (departmental)
} mailto:sryazant-at-ucla.edu
}
}





From daemon Sat Dec 21 21:16:55 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Sat, 21 Dec 2002 20:58:14 -0600
Subject: Glass Beading to Clean

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Hi guys,

I have used this technique to clean a penning guage and other parts (but
NEVER use it on gun parts in microscopes) and all the precautions listed are
to be taken. However, after using glass beading the first time, I think
that it gets dirty faster and the new texture of the metal surface makes it
harder to clean any other way. Watch out using higher pressures as the
metal can become deformed very easily.

Damian Neuberger, Ph.D.
Baxter Healthcare Corp.




From daemon Sun Dec 22 03:13:09 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 22 Dec 2002 01:12:12 -0800
Subject: RE: Evaporator cold trap

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Allen

DV502A has 800 l/sec DP!!! It enough to pump down a huge volume. If you
don't have any leaks in your system, the vacuum would be directly dependent
from the ultimate vacuum for the pump (which is somehow dependent from
pump's actual construction/quality and DP Oil). In most cases leaks are the
reason of vacuum degradation. Any normally serviced vacuum evaporator
should deliver at least very good 10-6 torr. 10-5 is very bad and actually
you may not use it for biological sample preparation. In my particular
case, I've replaced ALL O-rings on viton with touch of Apiezon L, cleaned
and polished DP and used Santavac-5. Santavac-5 is great DP oil. You have
to try. On my DV502A I have 2*10-6 after about 40 min pumping (no LN2) and
better than 5*10-7 overnight. I am using MKS cold cathode gauge with
sensitivity up to 10-9 torr (which I don't need). I think, the vacuum
quality is mostly a function of how clean your system and how many 10-year
old cracked "buna" O-rings is inside your system. The problem with "buna"
- it does not hold the shape and easily deformed even if it's not old. It's
also destroyed by vacuum grease (does not matter what manufacturers told
you). Viton is much better. Since I spent $500 on Santavac-5 8 years ago,
I never touch my DP again. By the way, I have another vacuum system, which
I build by myself. It has exact the same volume and similar amount of
O-rings but 400 l/s TP from SEIKO. That system is oil-free. I mean, it
has scroll pump as a backing device and TP itself does not have any oil
(it's magnetically levitated beauty). So, when I build the system, I was
expecting similar productivity for this system as for DV502A but
oil-free. I was completely wrong! This "baby" easily delivered to me
5*10-7 torr in 20 (yes, twenty) min! After a few hours, it's going into
10-8. So, my "theory" is that in the standard setup, oil from mechanical
pump contaminated the whole system (and your sample!) and adsorbs a lot of
air, which slowly released during the high vacuum pumping cycle. Because
my new system is oil-free, it's much faster. It has 12x12" Bell Jar with
6' collar. Another example: my old Polaron with 100 l/s DP (Santavac-5
again) and 12x12" Bell Jar. This system is very comfortable with 2*10-6
(no LN2) after I repaired manufacturers defect in DP. All tricks how to get
good vacuum perfectly described in the Wil Bigelow book. Have a great
Holidays (don't start cleaning DP- it's messy)! Sergey


At 08:13 PM 12/20/02 -0600, you wrote:
} Sergey,
}
} Have you verified that vacuum level with an independent, calibrated vacuum
} gauge? Vacuum sounds way to high for that system. I agree with all of
} your recommendations, but case in point, the vacuum you claim is on an
} order of what we normally find in a valve isolated electron gun (much
} smaller vacuum and seal volumes) pumped by a 20 L/sec ion pump. The vacuum
} level you stated for the start of your work is far closer to the best I
} have seen out of this particular evaporator after a complete rebuild.
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} On Friday, December 20, 2002 3:01 PM, Sergey Ryazantsev
} [SMTP:sryazant-at-ucla.edu] wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Randy
} } I am sorry for late comments. I agree with Wil Bigelow that LN2 trap will
} } reduce the pump performance. If you need just to increase the system's
} } overall performance, I would suggest you have to do very good service for
} } it first (replace all suspicious O-rings, clean everything up). I highly
} } recommend to use Santovac-5 DP (I assume, it's DP based system, because
} TP
} } don't need LN2). When I come in UCLA, I had DV-502A vacuum system with
} } 5*10-5 torr. I cleaned up DP (messy work and a lot of solvents),
} replaced
} } all O-rings (the system was 10 y.o. at the moment) and used
} } Santovac-5. Since that I do have 5*10-7 with no LN2 (and no further
} } service for more than 5 years). As an alternative, you may install some
} } "cold finger" with protective screens near your sample in the Bell
} } Jar. You really need LN2 trap over DP if you pump a lot of water in your
} } experiments. Sergey
} }
} } At 06:09 PM 12/16/02 -0600, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi All,
} } } I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to put a LN2
} cold
} } } trap
} } } on it, in an attempt to clean up the vacuum a tad more. There is a 3.5
} inch
} } } by 4.5 inch plate right above the diffusion stack which looks to be a
} great
} } } place to attach a cold trap. Rumor has it that this was an option. Are
} there
} } } any old traps kicking around that one can acquire? I could have our
} machine
} } } shop manufacture one, but was hoping for a cheaper route.
} } } Thanks in advance,
} } } Randy Nessler
} } } 319-335-8142
} }
} } ------------------------------------------------------
} }
} } Sergey Ryazantsev, Ph.D.
} } Electron Microscopy
} } Department of Biological Chemistry, School of Medicine
} } University of California, Los Angeles
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } (310) 825-1144 (office)
} } Pager: (310) 845-0248
} } FAX: (310) 206-5272 (departmental)
} } mailto:sryazant-at-ucla.edu
} }
} }

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Sun Dec 22 05:34:25 2002



From: Allen Sampson :      ars-at-sem.com
Date: Sun, 22 Dec 2002 05:26:03 -0600
Subject: RE: Evaporator cold trap

Contents Retrieved from Microscopy Listserver Archives
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Sergey,

That is a strong pump for this system - the normally configured system uses
a 250 L/sec pump. A diffusion pump, as any pump, is a differential system
- The ultimate pressure achieved is a balance between the leak rate in the
input, the back pressure in the output and the bypass rate in the pump (how
much gas can pass from the output to the input for a given pressure
differential, giving rise to the mechanical pump oil contamination in a
system). Although these pumps are rated up to 10-9 pressures, this is
never seen in operational systems. If you were to cap the diffusion pump
with a metal sealed, solid metal cap and had an enormous backing pump, you
may come close to the rated ultimate rating. In practical systems, these
levels are but a dream.

I'd be interested in knowing what roughing pump you are using.

No vacuum system has no leaks, particularly when elastomeric seals are
used. The standard Denton, with its manual valves and bell jar seal, has a
lot of elastomeric seals. And a cold cathode vacuum gauge accuracy is
debatable at 10-6 or less.

I've only recommended and used Santovac 5 for over twenty years. It is a
great diffusion pump oil. My main reason for recommending it is not the
ultimate vacuum attainable (debatable considering the greater temperature
apparently needed by Santovac, although I've never had a problem using it
in any diffusion pump), but rather it's relative insensitivity to sudden
air inrushes when hot. That means that it will 'crack' and polymerize less
than other oils. In other words - it will last longer and cause less hard
deposits on the pump.

As far as o-rings, I've found Buna to be quite acceptable, lightly coated
with Brayco, for static seals. Where dynamic seals are used (rotational or
translational forces are common), I use Buna with Apiezon (the waxier
Apiezon has more staying power, although it will also hold particulate
contaminants more). The reason for cracked o-rings is neglect, not the
suitability of vacuum greases. The reason for greasing o-rings is to
provide a light coating that preserves the qualities of the elastomeric
material, not to make up for insufficiencies in the vacuum sealing
surfaces. In this respect, the better vacuum greases do a good job and do
not compromise either Buna or Viton. I have many 25+ year old systems that
have original o-rings that are indistinguishable from new, in appearance,
shape or conformance.

The deformability of Buna is actually a plus, at least in systems that are
mostly held at vacuum. You may have noticed that a system that was just
rebuilt with new or rebuilt o-rings takes some time to come to an ultimate
vacuum equilibrium. That, of course, involves the outgassing of the system
components after being exposed to atmosphere for some time during the
rebuild. But it also includes the time required for the elastomeric vacuum
seals to be 'sucked' into place. A well designed o-ring seal will depend
on the mechanical pressures on the o-ring, but will ultimately depend on
the o-ring's conformance under the gas pressures it's subjected to.

In my experience, the Buna o-rings will deform to provide a good seal
faster and, properly maintained, will continue to conform to a shape that
best seals. I generally use Viton for it's improved resistance to high
temperatures. In either case, I use an acetone wipe for cleaning o-rings
every time I recondition them. It tends to swell the o-rings with two
effects - it restores them to their original shape and helps to provide a
quick seal when the system is pumped down again.

BTW, I've cleaned more DPs and refurbished more vacuum systems than I'd
like to elucidate. In my business, I tend to get the instruments that the
manufacturers don't service anymore, didn't service properly or have been
neglected for some time.

Just a few ruminations from a long career of servicing many vacuum
instruments.


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Sunday, December 22, 2002 3:12 AM, Sergey Ryazantsev
[SMTP:sryazant-at-ucla.edu] wrote:
} Allen
}
} DV502A has 800 l/sec DP!!! It enough to pump down a huge volume. If you
} don't have any leaks in your system, the vacuum would be directly
dependent
} from the ultimate vacuum for the pump (which is somehow dependent from
} pump's actual construction/quality and DP Oil). In most cases leaks are
the
} reason of vacuum degradation. Any normally serviced vacuum evaporator
} should deliver at least very good 10-6 torr. 10-5 is very bad and
actually
} you may not use it for biological sample preparation. In my particular
} case, I've replaced ALL O-rings on viton with touch of Apiezon L, cleaned
} and polished DP and used Santavac-5. Santavac-5 is great DP oil. You
have
} to try. On my DV502A I have 2*10-6 after about 40 min pumping (no LN2)
and
} better than 5*10-7 overnight. I am using MKS cold cathode gauge with
} sensitivity up to 10-9 torr (which I don't need). I think, the vacuum
} quality is mostly a function of how clean your system and how many
10-year
} old cracked "buna" O-rings is inside your system. The problem with
"buna"
} - it does not hold the shape and easily deformed even if it's not old.
It's
} also destroyed by vacuum grease (does not matter what manufacturers told
} you). Viton is much better. Since I spent $500 on Santavac-5 8 years
ago,
} I never touch my DP again. By the way, I have another vacuum system,
which
} I build by myself. It has exact the same volume and similar amount of
} O-rings but 400 l/s TP from SEIKO. That system is oil-free. I mean, it
} has scroll pump as a backing device and TP itself does not have any oil
} (it's magnetically levitated beauty). So, when I build the system, I was
} expecting similar productivity for this system as for DV502A but
} oil-free. I was completely wrong! This "baby" easily delivered to me
} 5*10-7 torr in 20 (yes, twenty) min! After a few hours, it's going into
} 10-8. So, my "theory" is that in the standard setup, oil from mechanical
} pump contaminated the whole system (and your sample!) and adsorbs a lot
of
} air, which slowly released during the high vacuum pumping cycle. Because
} my new system is oil-free, it's much faster. It has 12x12" Bell Jar with
} 6' collar. Another example: my old Polaron with 100 l/s DP (Santavac-5
} again) and 12x12" Bell Jar. This system is very comfortable with 2*10-6
} (no LN2) after I repaired manufacturers defect in DP. All tricks how to
get
} good vacuum perfectly described in the Wil Bigelow book. Have a great
} Holidays (don't start cleaning DP- it's messy)! Sergey
}
}
} At 08:13 PM 12/20/02 -0600, you wrote:
} } Sergey,
} }
} } Have you verified that vacuum level with an independent, calibrated
vacuum
} } gauge? Vacuum sounds way to high for that system. I agree with all of
} } your recommendations, but case in point, the vacuum you claim is on an
} } order of what we normally find in a valve isolated electron gun (much
} } smaller vacuum and seal volumes) pumped by a 20 L/sec ion pump. The
vacuum
} } level you stated for the start of your work is far closer to the best I
} } have seen out of this particular evaporator after a complete rebuild.
} }
} } Allen R. Sampson
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} }
} } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
} }
} }
} } On Friday, December 20, 2002 3:01 PM, Sergey Ryazantsev
} } [SMTP:sryazant-at-ucla.edu] wrote:
} } }
------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
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Q.html
} } }
-----------------------------------------------------------------------.
} } }
} } }
} } } Randy
} } } I am sorry for late comments. I agree with Wil Bigelow that LN2 trap
will
} } } reduce the pump performance. If you need just to increase the
system's
} } } overall performance, I would suggest you have to do very good service
for
} } } it first (replace all suspicious O-rings, clean everything up). I
highly
} } } recommend to use Santovac-5 DP (I assume, it's DP based system,
because
} } TP
} } } don't need LN2). When I come in UCLA, I had DV-502A vacuum system
with
} } } 5*10-5 torr. I cleaned up DP (messy work and a lot of solvents),
} } replaced
} } } all O-rings (the system was 10 y.o. at the moment) and used
} } } Santovac-5. Since that I do have 5*10-7 with no LN2 (and no further
} } } service for more than 5 years). As an alternative, you may install
some
} } } "cold finger" with protective screens near your sample in the Bell
} } } Jar. You really need LN2 trap over DP if you pump a lot of water in
your
} } } experiments. Sergey
} } }
} } } At 06:09 PM 12/16/02 -0600, you wrote:
} } }
} ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi All,
} } } } I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to put a
LN2
} } cold
} } } } trap
} } } } on it, in an attempt to clean up the vacuum a tad more. There is a
3.5
} } inch
} } } } by 4.5 inch plate right above the diffusion stack which looks to be
a
} } great
} } } } place to attach a cold trap. Rumor has it that this was an option.
Are
} } there
} } } } any old traps kicking around that one can acquire? I could have our
} } machine
} } } } shop manufacture one, but was hoping for a cheaper route.
} } } } Thanks in advance,
} } } } Randy Nessler
} } } } 319-335-8142
} } }
} } } ------------------------------------------------------
} } }
} } } Sergey Ryazantsev, Ph.D.
} } } Electron Microscopy
} } } Department of Biological Chemistry, School of Medicine
} } } University of California, Los Angeles
} } } Box 951737
} } } Los Angeles, CA 90095-1737
} } }
} } } (310) 825-1144 (office)
} } } Pager: (310) 845-0248
} } } FAX: (310) 206-5272 (departmental)
} } } mailto:sryazant-at-ucla.edu
} } }
} } }
}
} ------------------------------------------------------
}
} Sergey Ryazantsev, Ph.D.
} Electron Microscopy
} Department of Biological Chemistry, School of Medicine
} University of California, Los Angeles
} Box 951737
} Los Angeles, CA 90095-1737
}
} (310) 825-1144 (office)
} Pager: (310) 845-0248
} FAX: (310) 206-5272 (departmental)
} mailto:sryazant-at-ucla.edu
}
}
}



From daemon Sun Dec 22 21:02:45 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Sun, 22 Dec 2002 22:02:40 -0500
Subject: Re: Cleaning a Penning Gauge / abrasive blasting

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With the correct choice of abrasive size and hardness, and the right choice of
operating pressure and distance from the nozzle, abrasive cleaners can be
helpful tools. However, in using one you should be aware that this
microscopic "shot peening" of the surface has other effects on metals,
including work hardening and distortion.

For example, in microelectronics bright-plated gold is notoriously hard to
form a reliable polymer bond to. To overcome this problem I once worked with
a process that required micro-abrasive roughening of the plating surface in
the bottom of a flat Kovar package about the size of a matchbook. In the
course of 30 seconds of abrasive blasting, the thin bottom of this package
would acquire a visible bow due to its lateral expansion within the confining
sidewalls.

It may seem counter-intuitive, but the direction of the bow was toward the
abrasive jet rather than away from it, due to expansion of that surface
relative to the unabraded rear.

John Twilley
Conservation Scientist

Bill Tivol wrote:

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} -----------------------------------------------------------------------.
}
} On Friday, December 20, 2002, at 04:58 AM, qualityimages wrote:
}
} } If you have access to a bead blaster (sand blaster that uses fine
} } glass beads), it's a lot less work than SiC. Any aluminum parts
} } should be done with a reduced pressure of 35-40 psi or you may find
} } that the aluminum disappears pretty quickly.
}
} } Ken Converse
} }
} Dear Ken and Robert,
} The instrument I used, called an "air eraser", offered a choice of
} glass beads, corundum, or, I think, starch grains (in any case,
} something pretty soft) to use as the abrading particles. The soft
} abraders could be useful for Al; however, the dirt may be tougher than
} the Al, making the process unsuitable. The air eraser is ~$100, and
} it's useful for a number of tasks.
} Yours,
} Bill Tivol
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu





From daemon Mon Dec 23 08:40:23 2002



From: Lauren :      simmerman_2000-at-netzero.com
Date: Mon, 23 Dec 2002 14:19:21 GMT
Subject: TEM-Problem Nerve

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Hi Everyone,

We wanted to thank everyone who sent in suggestions for "problem nerves".
We ended up having very good luck with Karen Bentley's suggestion.

We cut at 1000nm, place the section on a drop of water, and then place a drop of 100% xylene on top. We then drain the slide and let it dry upright, & then put it on the hot plate to stain.

The doctors are very pleased with our new and improved results!

Thanks again----
The techs. at Nebraska Health System

Merry Christmas and A Happy New Year!





From daemon Tue Dec 24 07:29:42 2002



From: Sonja.Foubert-at-ua.ac.be
Date: Tue, 24 Dec 2002 14:15:55 +0100 (MET)
Subject: SEM Folds in CPD arabidopsis tissue

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} } Hello
} }
} } Can anyone give me information on how to prepare good CPD arabidopsis
} } roots and hypocotyls for FESEM.
} } After fixation in 4% formaldehyde in 0.5x PEM buffer (Pipes,EGTA,MgSO4),
} } samples were washed in 0.5x PEM buffer,cryoprotected in DMSO 25% and
} } 50%,cryosectioned by 120°C, thawed in DMSO 50%. Extraction was done
} } with 3%
} } sodium hypochlorite and 0.1 % pectolyase. Dehydration in 30% ....100%
} } ethanol and finally CPD (Balzers CPD 010) with liquid CO2 (10 changes
} } each 3 min and another 20 changes each 7 min.)
} } The cellulose fibrils are nice but the cell walls are not flat. A LOT of
} } FOLDS are visible. I guess this must be an artefact from CPD.
} }
} } Any suggestions for good CPD methods on plant tissue and especially on
} } extracted plant tissue are welcome
} }
} }
} } Thanks in advance
} }
} } S. Foubert





From daemon Wed Dec 25 19:29:20 2002



From: qualityimages :      qualityimages-at-netrax.net
Date: Wed, 11 Dec 2002 18:26:40 -0500
Subject: digital image capture systems

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-------- Original Message --------


Dear listers,
I have a friend who has about 10 customers looking for a digital image
capture system for their SEMs. I know several vendors are frequenters
of this list, but I'm not at all sure that it's inclusive. Does anyone
have a really complete list of digital image capture vendors, perhaps
even broken down by active vs. passive systems?

Thanks,
Ken Converse
owner
Quality Images
third party SEM service
Delta, PA









From daemon Thu Dec 26 10:50:52 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 26 Dec 2002 10:30:15 -0600
Subject: Re: SEM Folds in CPD arabidopsis tissue

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Hi,
Well the first thing I would do would be to get rid of the
EGTA in your fixation fixation buffer. Calcium generally strengthens
plant structure. Of course you are going to extract your pectins
later, so this might not make much difference. But it will simplify
things.

EGTA got put into fixation buffers for fixing plant cells in
the mistaken idea that it 'stabilizes' microtubules. But since
papers reporting tubulin biochemistry in vitro include EGTA in
buffers to show dilution-induced depolymerization, EGTA can hardly be
said to stabilize microtubules. Certainly not in the way in which
taxol stabilizes microtubules. Furthermore, Calcium is a component of
membranes and when you extract it at the time of fixation you damage
your membranes at the same time as fixation, which tends to be bad
news for good structural preservation. If you want to extact calcium
to take out pectins, the time to do that is after fixation.

Honestly, this probably won't help your folding problem. But
it might, and it will help other fixations if you do them in the
presence of EGTA.

Good luck,
Tobias Baskin


} ------------------------------------------------------------------------
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--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123
http://www.biosci.missouri.edu/Baskin/baskin_lab__home_page.htm


From daemon Mon Dec 30 09:22:25 2002



From: Beveridge, Mark J. :      bevermj-at-peds.ufl.edu
Date: Mon, 30 Dec 2002 09:56:02 -0500
Subject: paraformaldehyde fixation

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i would like to know if there is any way to unfix tissue fixed in
paraformaldehyde for immunoblotting?


From daemon Mon Dec 30 13:15:14 2002



From: saram-at-duke.edu
Date: Mon, 30 Dec 2002 14:04:02 -0500 (EST)
Subject: Re: paraformaldehyde fixation

Contents Retrieved from Microscopy Listserver Archives
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It depends on what you mean by "unfix" and what you want to do with the
tissue after you unfix it.

Aldehydes are somewhat reversible and a portion can can simply be washed
out. This is why you don't want to leave tissue in buffer after aldehyde
fixation, but can store it in glut for a while without damaging
ultrastructure.

Aldehydes can also be "quenched" by certain agents such as ammonium
chloride or glycine for immunostaining the tissue.

Some tissues actually work better in some immunostains if they are fixed
because some antibodies are made with denatured proteins.

If you mean "completely return the tissue to its unfixed state", I'm
afraid the answer is "no".


Sara Miller

On Mon, 30 Dec 2002, Beveridge, Mark J. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} i would like to know if there is any way to unfix tissue fixed in
} paraformaldehyde for immunoblotting?
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265


From daemon Tue Dec 31 04:35:51 2002



From: Kenneth Tiekotter :      tiekotte-at-up.edu
Date: Tue, 31 Dec 2002 02:20:06 -0800 (PST)
Subject: DMC2 to PowerBook

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Dear All,

Have any of you successfully connected a Polaroid DMC2 camera to an
Apple PowerBook via a Belkin firewire to scsi converter running on OS X
and OS 9 via Photoshop 6?

Thank you for your assistance. Bless wishes for a healthy and safe
2003!

Ken

---------------------------------------
Kenneth L, Tiekotter, Adjunct Professor
Dept. of Biology
The University of Portland
5000 N Willamette Blvd,
Portland, OR 97203 USA


From daemon Tue Dec 31 18:31:55 2002



From: Lou Ross :      RossLM-at-missouri.edu
Date: Tue, 31 Dec 2002 18:16:51 -0600
Subject: SEM examination of wood cells

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Hi,

We have a researcher on campus who would like to examine the cell
size of 3000 year old wood. Any ideas on the sample preparation
protocol to preserve the cell structure without shrinking?

Please direct your responss to Cheryl Jensen (jensenc-at-missouri.edu).

Thanks in advance.
Lou Ross
--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.biotech.missouri.edu/emc


From daemon Fri Aug 1 12:34:16 2003



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Fri, 1 Aug 2003 12:27:34 -0500
Subject: Administrivia: This is a component test

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Sorry Colleagues, but this is a test

Nestor
Your Friendly Neighborhood SysOp






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